LOCUS       DALAGF010000001        99795 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.001, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000001 DALAGF010000000
VERSION     DALAGF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 99795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99795
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.001"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(217..1386)
                     /locus_tag="O2692_000001"
     CDS             complement(217..1386)
                     /locus_tag="O2692_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol--glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HCZ4297653.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQNAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTIFK"
     gene            complement(1814..3508)
                     /locus_tag="O2692_000002"
     CDS             complement(1814..3508)
                     /locus_tag="O2692_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCZ4297654.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKENITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     FESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            complement(3530..4555)
                     /locus_tag="O2692_000003"
     CDS             complement(3530..4555)
                     /locus_tag="O2692_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCZ4297655.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKTYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            complement(4548..5264)
                     /locus_tag="O2692_000004"
     CDS             complement(4548..5264)
                     /locus_tag="O2692_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCZ4297656.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            complement(5792..6835)
                     /locus_tag="O2692_000005"
     CDS             complement(5792..6835)
                     /locus_tag="O2692_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCZ4297657.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIIKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            complement(6859..7005)
                     /locus_tag="O2692_000006"
     CDS             complement(6859..7005)
                     /locus_tag="O2692_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297658.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            complement(7007..8062)
                     /locus_tag="O2692_000007"
     CDS             complement(7007..8062)
                     /locus_tag="O2692_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCZ4297659.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAYHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKVLLKPLDLDENEGEN"
     gene            complement(8080..9339)
                     /locus_tag="O2692_000008"
     CDS             complement(8080..9339)
                     /locus_tag="O2692_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCZ4297660.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQIICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            complement(9566..9844)
                     /locus_tag="O2692_000009"
     CDS             complement(9566..9844)
                     /locus_tag="O2692_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCZ4297661.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            complement(9867..10334)
                     /locus_tag="O2692_000010"
     CDS             complement(9867..10334)
                     /locus_tag="O2692_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCZ4297662.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            complement(10319..12415)
                     /locus_tag="O2692_000011"
     CDS             complement(10319..12415)
                     /locus_tag="O2692_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCZ4297663.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLENHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDSISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRMHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            complement(12546..12644)
                     /locus_tag="O2692_000012"
     CDS             complement(12546..12644)
                     /locus_tag="O2692_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297664.1"
                     /translation="MYVKNASKKHKTQKYMQKFQLLYFKRLKSQKI"
     gene            complement(12754..13689)
                     /locus_tag="O2692_000013"
     CDS             complement(12754..13689)
                     /locus_tag="O2692_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCZ4297665.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            14051..15580
                     /locus_tag="O2692_000014"
     CDS             14051..15580
                     /locus_tag="O2692_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCZ4297666.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            complement(15900..16853)
                     /locus_tag="O2692_000015"
     CDS             complement(15900..16853)
                     /locus_tag="O2692_000015"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCZ4297667.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYEEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(17164..17271)
                     /locus_tag="O2692_000016"
     CDS             complement(17164..17271)
                     /locus_tag="O2692_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297668.1"
                     /translation="MSVCTPTIERIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            17426..18571
                     /locus_tag="O2692_000017"
     CDS             17426..18571
                     /locus_tag="O2692_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HCZ4297669.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGKNL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQV
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            18597..18770
                     /locus_tag="O2692_000018"
     CDS             18597..18770
                     /locus_tag="O2692_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297670.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            18927..19283
                     /locus_tag="O2692_000019"
     CDS             18927..19283
                     /locus_tag="O2692_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCZ4297671.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
     gene            19481..20956
                     /locus_tag="O2692_000020"
     CDS             19481..20956
                     /locus_tag="O2692_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ4297672.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSFENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(21270..22412)
                     /locus_tag="O2692_000021"
     CDS             complement(21270..22412)
                     /locus_tag="O2692_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HCZ4297673.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMLSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVVLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            22853..24430
                     /locus_tag="O2692_000022"
     CDS             22853..24430
                     /locus_tag="O2692_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCZ4297674.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEIYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
     gene            24456..25961
                     /locus_tag="O2692_000023"
     CDS             24456..25961
                     /locus_tag="O2692_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HCZ4297675.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDNDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILNQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            26073..27284
                     /locus_tag="O2692_000024"
     CDS             26073..27284
                     /locus_tag="O2692_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HCZ4297676.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            27470..29731
                     /locus_tag="O2692_000025"
     CDS             27470..29731
                     /locus_tag="O2692_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HCZ4297677.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKISIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDTIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPISHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            29761..30945
                     /locus_tag="O2692_000026"
     CDS             29761..30945
                     /locus_tag="O2692_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCZ4297678.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GFKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            31259..31366
                     /locus_tag="O2692_000027"
     CDS             31259..31366
                     /locus_tag="O2692_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297679.1"
                     /translation="MCVRTRLVSSSSARLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(31548..33377)
                     /gene="coa"
                     /locus_tag="O2692_000028"
     CDS             complement(31548..33377)
                     /gene="coa"
                     /locus_tag="O2692_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HCZ4297680.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKN
                     GTLIDSRYLNSALYYLEDYIIYAIGLTNKYEYGDNIYKEAKDRLLEKVLREDQYLLER
                     KKSQYEDYKQWYANYKKENPRTDLKMANFHKYNLEELSMKEYNELQDALKRALDDFHR
                     EVKDIKDKNSDLKTFNAAEEDKATKEVYDLVSEIDTLVVSYYGDKDYGEHAKELRAKL
                     DLILGDTDNPHKITNERIKKEMIDDLNSIIDDFFMETKQNRPKSITKYNPTTHNYKTN
                     SDNKPNFDKLVEETKKAVKEADDSWKKKTVKKYGETETKSPVVKEEKKVEEPQAPKVD
                     NQQEVKTTAGKAEETTQPVAQPLVKIPQGTITGEIVKGPEYPTMENKTVQGEIVQGPD
                     FLTMEQSGPSLSNNYTNPPLTNPILEGLEGSSSKLEIKPQGTESTLKGTQGESSDIEV
                     KPQATETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETN
                     AYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHGNGQVSYGARPTQNKPSKTNAYNV
                     TTHANGQVSYGARPTYKKPSKTNAYNVTTHADGTATYGPRVTK"
     gene            33567..33911
                     /locus_tag="O2692_000029"
     CDS             33567..33911
                     /locus_tag="O2692_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HCZ4297681.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKQRLESIY
                     NSISNPLHSQNN"
     gene            complement(34075..35838)
                     /locus_tag="O2692_000030"
     CDS             complement(34075..35838)
                     /locus_tag="O2692_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HCZ4297682.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWTEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDIVGLKMRQNGHEAKFVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(36174..36929)
                     /gene="pflA"
                     /locus_tag="O2692_000031"
     CDS             complement(36174..36929)
                     /gene="pflA"
                     /locus_tag="O2692_000031"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCZ4297683.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            complement(36952..39201)
                     /gene="pflB"
                     /locus_tag="O2692_000032"
     CDS             complement(36952..39201)
                     /gene="pflB"
                     /locus_tag="O2692_000032"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCZ4297684.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            39789..40757
                     /locus_tag="O2692_000033"
     CDS             39789..40757
                     /locus_tag="O2692_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCZ4297685.1"
                     /translation="MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            40754..42310
                     /locus_tag="O2692_000034"
     CDS             40754..42310
                     /locus_tag="O2692_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCZ4297686.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     NRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            42303..43061
                     /locus_tag="O2692_000035"
     CDS             42303..43061
                     /locus_tag="O2692_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4297687.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(43420..44799)
                     /gene="uhpT"
                     /locus_tag="O2692_000036"
     CDS             complement(43420..44799)
                     /gene="uhpT"
                     /locus_tag="O2692_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCZ4297688.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            45032..45526
                     /locus_tag="O2692_000037"
     CDS             45032..45526
                     /locus_tag="O2692_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HCZ4297689.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            complement(45888..46856)
                     /locus_tag="O2692_000038"
     CDS             complement(45888..46856)
                     /locus_tag="O2692_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCZ4297690.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVSESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(46911..47951)
                     /locus_tag="O2692_000039"
     CDS             complement(46911..47951)
                     /locus_tag="O2692_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCZ4297691.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            complement(47976..49052)
                     /locus_tag="O2692_000040"
     CDS             complement(47976..49052)
                     /locus_tag="O2692_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCZ4297692.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            complement(49126..49965)
                     /locus_tag="O2692_000041"
     CDS             complement(49126..49965)
                     /locus_tag="O2692_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCZ4297693.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            complement(49967..51235)
                     /locus_tag="O2692_000042"
     CDS             complement(49967..51235)
                     /locus_tag="O2692_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCZ4297694.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYIINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            complement(51238..52509)
                     /locus_tag="O2692_000043"
     CDS             complement(51238..52509)
                     /locus_tag="O2692_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCZ4297695.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            complement(52522..53619)
                     /gene="ugpC"
                     /locus_tag="O2692_000044"
     CDS             complement(52522..53619)
                     /gene="ugpC"
                     /locus_tag="O2692_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCZ4297696.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            complement(54002..54580)
                     /locus_tag="O2692_000045"
     CDS             complement(54002..54580)
                     /locus_tag="O2692_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCZ4297697.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGIQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            54789..55415
                     /locus_tag="O2692_000046"
     CDS             54789..55415
                     /locus_tag="O2692_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCZ4297698.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            55605..56378
                     /locus_tag="O2692_000047"
     CDS             55605..56378
                     /locus_tag="O2692_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ4297699.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGDMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDKKNATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQS
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPKNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKDQIIYKESLTYNKISEH"
     gene            complement(56676..58682)
                     /gene="ggt"
                     /locus_tag="O2692_000048"
     CDS             complement(56676..58682)
                     /gene="ggt"
                     /locus_tag="O2692_000048"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCZ4297700.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVLEPFASGIGGGGYLLYYEQSTGSITAFDARETAPEHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLAKYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIAHGHAME
                     HKPDAYIGDVHGLQVDPTTYEASGGSDDTREGTVMGGEVLVIRKQPLPYRQMYDSDGF
                     RLYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFPEVSAHIEDLRSYENAGENYIDIA
                     WLARKYAYQVTLKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(58720..60495)
                     /locus_tag="O2692_000049"
     CDS             complement(58720..60495)
                     /locus_tag="O2692_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCZ4297701.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSHDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            complement(60512..61675)
                     /locus_tag="O2692_000050"
     CDS             complement(60512..61675)
                     /locus_tag="O2692_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4297702.1"
                     /translation="MAQLNSKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            complement(61681..63159)
                     /locus_tag="O2692_000051"
     CDS             complement(61681..63159)
                     /locus_tag="O2692_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4297703.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     EQALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENVLFHKFI
                     MIKVILALPMFILLTFYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALMVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            63116..64708
                     /locus_tag="O2692_000052"
     CDS             63116..64708
                     /locus_tag="O2692_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4297704.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLNNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVIFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQTFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            complement(64973..66085)
                     /locus_tag="O2692_000053"
     CDS             complement(64973..66085)
                     /locus_tag="O2692_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HCZ4297705.1"
                     /translation="MKICEKCFNNTEIVEIIANDNSKFDNCDIDNNHLGVKIFDTTKD
                     IDKLELIRDYLRPALELYDISINLPDTFSPKEGKKIEIALKDDWSIFNVEEAQISCIL
                     NELFKDDENLDRRVLEDLVGAKIINDKKYTNKNLIVANNDWDGFCESLKYKNRFHNNM
                     INLENLAFFLDITTNYYSIEEFRERFEPLYRSRIVKFITEKSELMSPPKGFATAGRLN
                     SKWISVLYLSTKEQVSIEEVKPKHNDIIYIGKVKLQKNVTKEKLKIANLTNLTSNAIK
                     AGDDGFRKYFVNYQTLKKIHKGITNPSDEQGIDYLPFQYLADYIRSLKYDGIMYESIL
                     QDGTFNFVFFDQSLFECVNYERKRVSDVKYTLTKLS"
     gene            complement(66072..66995)
                     /locus_tag="O2692_000054"
     CDS             complement(66072..66995)
                     /locus_tag="O2692_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCZ4297706.1"
                     /translation="MYYPYLRGKQNELFAIRELLEKGLIGDCIQPIIEPIKYTTTFKN
                     TLQYCGEKAFSINLVVNSKLTEEEISNETVAHLTEIITKNKSVIQKAYLGPSDEGNDR
                     LKQQFSSNSLAILTSVDDWEMFGDKNKLEMVFVPDDRHIKRKLRNIPNKGIIMDPFNK
                     LSRNVDYLDNDDEFYSDDHLYYKEDGYVAFSDYSVIGGEYVDGGFSPLAIAIHIVYFD
                     EANELRVKHFVSDSNNDRSNPGKKFFEAVDKLVTWSKNLDIKNRSYALGQFEELNENN
                     KYPGLGLIKRLSIMHHLEIMNRYLESQNENM"
     gene            complement(66997..67839)
                     /locus_tag="O2692_000055"
     CDS             complement(66997..67839)
                     /locus_tag="O2692_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCZ4297707.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKSYRNEYFYKNTILNKLLLGRHSINTTTALSEMPIGKSIADFILLN
                     GKGVVYEIKTELDKLDRLDNQINDYYEVFNYVVVITNDKHLNKVMARYKDTTVGILVL
                     TTRNTLSEVQKPKENNSLLNTKAMYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFD
                     VFKEIPMTKLHNNMISELKKRGNMKEYKDEFLAAPAEIKFLLYFAKMTKKDKNKLYHF
                     LKED"
     gene            complement(68044..70833)
                     /locus_tag="O2692_000056"
     CDS             complement(68044..70833)
                     /locus_tag="O2692_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCZ4297708.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAPLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            71046..71327
                     /locus_tag="O2692_000057"
     CDS             71046..71327
                     /locus_tag="O2692_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297709.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
     gene            complement(71521..72396)
                     /locus_tag="O2692_000058"
     CDS             complement(71521..72396)
                     /locus_tag="O2692_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCZ4297710.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(72399..73853)
                     /locus_tag="O2692_000059"
     CDS             complement(72399..73853)
                     /locus_tag="O2692_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCZ4297711.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            complement(73865..74761)
                     /gene="murQ"
                     /locus_tag="O2692_000060"
     CDS             complement(73865..74761)
                     /gene="murQ"
                     /locus_tag="O2692_000060"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCZ4297712.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            complement(74761..75816)
                     /locus_tag="O2692_000061"
     CDS             complement(74761..75816)
                     /locus_tag="O2692_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCZ4297713.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYDHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSDLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            76402..78447
                     /locus_tag="O2692_000062"
     CDS             76402..78447
                     /locus_tag="O2692_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCZ4297714.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            complement(78426..78527)
                     /locus_tag="O2692_000063"
     CDS             complement(78426..78527)
                     /locus_tag="O2692_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297715.1"
                     /translation="MMLQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            78721..80361
                     /locus_tag="O2692_000064"
     CDS             78721..80361
                     /locus_tag="O2692_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HCZ4297716.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQGGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTATKYTDASTYIELLATKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNN
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMAVEDAPKKLIDIAKAFSQQNK"
     gene            80428..80985
                     /locus_tag="O2692_000065"
     CDS             80428..80985
                     /locus_tag="O2692_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCZ4297717.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPYTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
     gene            81261..82616
                     /gene="brnQ"
                     /locus_tag="O2692_000066"
     CDS             81261..82616
                     /gene="brnQ"
                     /locus_tag="O2692_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCZ4297718.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKLIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
     gene            82869..84053
                     /gene="rocD"
                     /locus_tag="O2692_000067"
     CDS             82869..84053
                     /gene="rocD"
                     /locus_tag="O2692_000067"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCZ4297719.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            84092..85123
                     /locus_tag="O2692_000068"
     CDS             84092..85123
                     /locus_tag="O2692_000068"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCZ4297720.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            85135..86376
                     /gene="argJ"
                     /locus_tag="O2692_000069"
     CDS             85135..86376
                     /gene="argJ"
                     /locus_tag="O2692_000069"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HCZ4297721.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            86392..87156
                     /gene="argB"
                     /locus_tag="O2692_000070"
     CDS             86392..87156
                     /gene="argB"
                     /locus_tag="O2692_000070"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCZ4297722.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            87426..87905
                     /locus_tag="O2692_000071"
     CDS             87426..87905
                     /locus_tag="O2692_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HCZ4297723.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(88233..88877)
                     /locus_tag="O2692_000072"
     CDS             complement(88233..88877)
                     /locus_tag="O2692_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCZ4297724.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(88890..96065)
                     /locus_tag="O2692_000073"
     CDS             complement(88890..96065)
                     /locus_tag="O2692_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HCZ4297725.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     VTPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPETIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSE
                     LNVAQLRQAVQRLIARHEILRTQYIVVDDEVRQRIVADVAVDFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHHCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQVEQDLKQYLNDQLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMYQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGNTLLTGATGFLGAYLIEVLQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEIMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTNPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(96512..97762)
                     /locus_tag="O2692_000074"
     CDS             complement(96512..97762)
                     /locus_tag="O2692_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4297726.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(98148..99173)
                     /locus_tag="O2692_000075"
     CDS             complement(98148..99173)
                     /locus_tag="O2692_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HCZ4297727.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(99346..99705)
                     /locus_tag="O2692_000076"
     CDS             complement(99346..99705)
                     /locus_tag="O2692_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCZ4297728.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
BASE COUNT        31945 a        18677 c        15586 g        33587 t
ORIGIN      
        1 ccaattgaaa actcatttta aaatcaaaac tctaaatgtc tgtgtattac ttaaaattat
       61 acatattttg cttatatttt agcatatttt gtttaaacct atattacatt atatcagacg
      121 ttttcataca caaataataa catacaagca aacatttcgt ttattattta tatcacttaa
      181 ctaattaatt tataattttt tattgttttt aagttatcac ttaaaaatcg tttggcaaat
      241 tcgttgtgac gcttgtccat cttctaatga acagaatttt tgataaaata ccgttcgtgc
      301 ttcaatatac tcatttgcag tctcatcgat ttgttttaat gcatcaatga gtgctgtttg
      361 attttcaaca attggacctg gcaactcttt tttataatcc atgtaaaaac ctctaagctc
      421 atcgccatat ttatctaagt catatgcata gaaaatttgc ggacgcttta atacaccgaa
      481 gtcgaacatg acagatgagt agtcggtaac taacgcatcg ctgattaagt ataaatccga
      541 aatgtcttca taatctgaaa cgtctttcac aaaatcatca tgttcatcaa tacgtgtcac
      601 aactaaataa tgcatgcgta ataaaataac ataatcatca tccagcgctt gacgcaaagc
      661 ttctatatca aagttaacat taaattgata tgaaccttct cgaatcgctt catcgtcacg
      721 ccaagttggc gcgtacataa tcactttttt atctaatgga atatttaatc ttgtcttaat
      781 accattaata tattcagtat cattgcgttt atgtgataat ttatcatttc ttggataacc
      841 tgtttccaaa atcttatctc gactaacatg aaatgcattt tgaaatatcg atgtcgaata
      901 tggattaggt gacactagat aatcccaccg ttggctttct tttttaaagc catcttggta
      961 attttgagta tttgttccta gcattttaac gttactaata tccaaaccaa tcttttttaa
     1021 tggcgtgcca tgccatgttt gtaagtacgt cgttcgcggt gatttatata accaatctgg
     1081 tgtacgtgtg ttaatcatcc acgctttcgc tcttggcatc gctaaaaacc atttcattga
     1141 aaactttgta acatatggta cattgtgctg ttggaatatg tgttcatatc cttttttcac
     1201 accccatatt aattgggcat cgctatgttc agttaagtat tcatataatg ctttggggtt
     1261 gtcgctgtat tgtttaccat gaaagctttc aaaataaatt agattcttgt ttggcaattt
     1321 tgatagtaat ttaaaagtcg tatatatact atgttctatc aattttttaa ttgtattttt
     1381 aatcatgtcg tacctccgac gtgtttttgt aattatatta atatgtatga gcaagctcat
     1441 tgtaaccatg cctattatag catttcatca taaaatacat ttaaccatta cacttgtcgt
     1501 taattatcat acgaaataca tgattaatgt accactttaa cataacaaaa aatcgttatc
     1561 cattcataac gtatgtgttt acacatttat gaattagata acgattggat cgattatttt
     1621 attttacaaa atgacaattc agttggaagg tgattgcttt tgattgaatc gccttatgca
     1681 tgaaaaatca aaaggttatt ctcattgtat agtcctgctt ctcatcatga catgttgctc
     1741 acttcattgt cagaaccctt cttgaaaact atgccttatg actcatttgc atggcaagta
     1801 atatatgcca acattagcgt ctaaacaaat ctttgactaa acgttcactt gagcgaccat
     1861 cttgatattt aaaatgttta tctaagaatg gcacaacttt ttcaacctca taatcttcat
     1921 tgtccaaagc atccattaat gcatcaaagg actgtacaat tttacctgga acaaatgatt
     1981 caaatggttc atagaaatca cgcgtcgtaa tgtaatcttc taagtcaaat gcatagaaaa
     2041 tcatcggctt tttaaatact gcatattcat atattaaaga tgaataatca ctaatcaaca
     2101 agtctgtaac aaagagaata tcgttaactt cacgatgatc tgacacatcg ataaagtatt
     2161 gtctatgttc acgtgaaata ttaagtctat tttttacgaa cggatgcatt ttgaataaca
     2221 caactgcatt atgcttctcg cagtatcttg ctaaacgttc aaaatcaatt ttaaaaaatg
     2281 gataatgtgc cgtaccgtga ccattacctc taaatgtcgg tgcgaataga ataactttct
     2341 taccttttat aattggcaat tcatcttcca tctcttgttt aatttgtgtt gcataagctt
     2401 catcaaatag tacatcagta cgtggtacac ctgttggtac aacattttct tctctaattc
     2461 caaatgcttc agcataaaat ggaatatcgg tttctgatga aacatatgct ttcgtgtaac
     2521 tacgatggtt taatgaatcg ataaacggac cacctttttt acctgtacga ctaaagccaa
     2581 ctgttttaaa ggcaccaaca gcatgccaca cttgaataat ttcttgtgat ggtctaaagc
     2641 gaaccgtata aattaatgga tggaaatcat caacaaaaat ataatcggcc ttaccaagta
     2701 aatatggcaa tctaaacttg tctctccatt tgcgtctatc cgtaatattc tccttaaata
     2761 ccgttttaat atcataatca aaatctactt tttggcgtag taattcatca tatacatact
     2821 tgaaattccc tgataaattc ggacgcgaat ctgatgtgaa taatattgtt ttgcttcttt
     2881 taatatgtag taactttgta atattaaaaa tagctttaaa taagaaactt ctactttcaa
     2941 atgaagcttt atgaccttgt ttatgaagcc agtgtgcact tgtcgcaatg acccctgatt
     3001 tctcttgagg taaggtgatt tcaatatcaa atacaaattc gttaacgtca cttgctattt
     3061 ccggagtaat ggtatagacc gtatgttgtg atacgccacc tttacggaaa actttagcat
     3121 catacgctaa taaatagttc acttttccat tttgtgtagt cagctcattg tattcttcaa
     3181 tttgctcttc atttaatgcc gcataagctt gatcaataat atttggatta agttgtccaa
     3241 tatactctaa ctggtcatga taaacaaata aatatttatc cattggtaaa taatacccat
     3301 cgtctaatat tgcgacgttg aatctacata taaatgtatt accgtctatt ttcatttcat
     3361 ttgcttctaa tgtttttgtt tcggttaaat tcctcagaac aaattgtcct gatgttaaat
     3421 cgacaccttc actatgtcct tcaatatata actgaacacg ttcccagtat atgttatcaa
     3481 tatgtattgc ttgtttcgtt tttgtcattg ttattccccc atcggtttat taaatcgtcc
     3541 atttcaatac tgtttttccc caagatgtcg ataaatccat ttcaaacgct tggacgatat
     3601 cttgcatcgt acatacatta atttcatgtc cttttaataa tgctaacttt tcaactatgt
     3661 ctgggtactt acgatataaa tcaacaactt gctcaaaatc tttagagccg cttcgactac
     3721 taccaatcaa cgttaatcct ttttcaagta ctaatcgtgt attcacttcc acgggtaatt
     3781 cacttacgcc taacaaagca atactgcctt ctggtgaaat atgttcaact atttgttgaa
     3841 gtgcaacttg acttccttta cctccaacac attcaaatgc atgatcaatt ttaagatcat
     3901 ctggtatttg atttactgta aagatgtcat ctacaaatga aaaatgactt aatttatagt
     3961 ctgtcttacc aaatacataa gttttagctt ctgggtacaa cttacgtagc aaaatagcag
     4021 taatataacc taagttacca tcaccccaaa taccaaagct ggttttcaaa ggtatagatt
     4081 tacgttcaaa tcgttgtata gcatgataac ttactgacac taactctgtg tatgaaatcg
     4141 tactcaaatc aatgtcatta ggcagcggaa cgatacgatc atgtgccatc acaacgtagt
     4201 cttgcataaa accatcataa ccactagatc taaaataact agaggctaag taattctccg
     4261 caataatatg atgttgctct gtaggtgtat tcggtaccat tactactttc gtaccttttt
     4321 caaatacccc tttactatca aatacaactt caccaacagc ttcatgaact aatgacattg
     4381 gtaatttttt gcgtagtaca ttttcatctc ttcgacctgt gtaatacctt tgatcagctg
     4441 cacaaataga caagtataaa ggtcttacga tgacatgatt accataaata tcaacattat
     4501 tatatgtgac gtcgaactgt ctcggtgcaa cgagttgata tacttgatta atcatcggca
     4561 atatcacctt gaataatggc atttgctact tttaaatcat acggtgttgt cactttaatg
     4621 ttgtatagtt ctccacgtac caatttaact gcatgtccag attcgacaat gattttacat
     4681 gcatctgata agatttcttt ttgttcacta cttaaggcgc gataactatc ttgtaataat
     4741 ttaatattaa atgattgtgg tgtttggcct tgatacattt cattccttac agggatactg
     4801 tgtatgttct gtttatcttt agacattaca atcgtatcaa ttgcttcaat gactgtatct
     4861 actgcaccat attttgctgc tacttcaatg ttctctttaa taatacgttg agttaaaaat
     4921 ggtcttacgg catcatgagt tacaatcaca tcatcattat taattccatt tacattgcga
     4981 atatggtcga taatgttcat aattgtttca tttcgatccg taccacctgc aactactttg
     5041 acacgttgat ctgtaatgtt atattttttt aaaatatcct gtgtatggga aatccactgt
     5101 gctggcgttg cgataataat ctcattaaat tcactcacta aaatgaactt ctcaattgta
     5161 tggattaaaa tcggtttatt atcaatatct aaaaattgtt ttggtaatgg cacgttcccc
     5221 attctcgaac caatacctcc tgctaaaata cctgcataaa tcatgttgtc ctccattctg
     5281 tcattacatc atttccattt atacattact gacctatgcc cgcacataag cctaacctat
     5341 tgctcacttg cctcttttat taatccaaag atagttgtca caatagtgtg ataatttttt
     5401 ataaaaatgt atttttgtaa ctgaccattc taagttgttt tgccatgcag ttaatcatta
     5461 actctgacga tattaaattg ttaaaggtat taatgtttac tctttttcaa attcattatt
     5521 actgccatca ttttaccata tattataata aatttatctt attaagtggc tgtacttgat
     5581 tttcacttta aaaattatca aatattgcca tctcatttta agtatacaaa atgcaaaaca
     5641 accgattcac aagcatattt cacacaagta aaccggctat ttatcaacgt atattcgaag
     5701 atgaattatt tcgatagtat ctatagacca gacggcattc gcactttcat agctataact
     5761 ataccagcgt tttcgtcctc aaaggtgcat actaataaat cgtaaacatg actttatcaa
     5821 atcgttcttt cttgttaact aatttatcaa atgtctccgg gcctttttct aacggtaaaa
     5881 aatgagaaat aataggcttt acattaatat ctttcgtctt catataatgt aaggttgccg
     5941 tccactcttt gcccggaaaa ttactggaca aacagttcca agagccacat actgtcaact
     6001 cgttacgcag aattttttca aaatgaacgc gatcaatctc aatatcatca tatggtattc
     6061 cgagtaatac cacctcgcca ccttttttag gtagcgtcaa tatttgacca atcgtaactt
     6121 tagcacctga tgattctata gctaaatcga tttgattggc gtaatgattt tcgatgaatt
     6181 tctcaagatt ttcttctttt gaattgattg tttgatgtgc gcccaatgat gttgcaatat
     6241 ctagtttatg cgcatctata tctatagcga tgatatgtgc agcaccaaat attcgtgccc
     6301 attgaatagc taacaaacct atactgccac accccattac tgcaacagtc ataccaggtt
     6361 gtatattcga tttataaaac ccatgcgcaa caacggctga tggctcaacc attgctgctt
     6421 caatgtaatc aacattgtct ggaaccttta aaacattttg cgctggcaat ttgacatatt
     6481 ccgcgaacga tccaggttca tatgagccaa tgacgaataa cttttcacat cgtgcatatt
     6541 caccttttaa acaatactcg cattgataac aaggtattgc tgggcaacct gtcactttgt
     6601 cgcccacatt aacatgcgta acatcacttc caatggcatc tactacacct gaaaattcat
     6661 gaccaaatgg cataccttta atgtatggcc ccattttttt gtatcgtgac gtgtctgaac
     6721 cacatatgcc agtcgctcgt actttaataa taacgtcatt cgcactttca atgactggct
     6781 tttcattatc ctcataccgt aaatcttcca cgccatataa tttcaatgct ttcacttgta
     6841 aatcacctca aatttgattt aattcacaac ttttttcttt ttaaaaatac ctgtcgcaaa
     6901 ataacctgca atgacaatgg aattacttac gagtaaatgt tccatataaa aatcagtgat
     6961 ttgtcttaat ggcccaagca taaaagttag cattgattct accatattaa ttctctcctt
     7021 cattttcatc taaatcgagc ggcttaagta atactttaat accttcgccg gattttaaat
     7081 gttgatacgc ctcgtcccat ttcgaaatat catatatttt tgtcaccaaa gcttcagcat
     7141 ttactaaacc atccgccata agttgcaatg aaggttccca atctgctggc ttttgacttc
     7201 tactaccaac aactgttatt tctttttgaa tcactttttc catatcaaat ggaatttcag
     7261 catccttaaa aatacctatt tgactgtaga aacctttttt gcgtaaaata tccaaacctt
     7321 gtcgtgctgc tggaactgca cctgaacatt caacaacaac atctgcaccg taaccgtctg
     7381 taattccatt gatatacgtt tttaagtctg tttgttgtaa attgactaca taatccatgt
     7441 gcaatgcttc tgctttatct aatctgactt tgtcattgtc caatccagtt accacaacag
     7501 ttgcgccttt actttttaac acttgtgcta caagtaatcc gattggccca ggtcccatta
     7561 caactgctac atcgcctgaa ttgacttgaa tcttagaaac gccatgatat gcacatgcta
     7621 atggttctgt catagctgca gactgatacg atacttcgtc tggaatatga tgcaaacttt
     7681 cttcacgtgc aatgacataa ttagtaaatg cgccatcaac ttgtgttcca ataccttttc
     7741 gatggttgca taaattatag tcttttgatt tacagtattc acactcatta caaacataga
     7801 atgtcgtttc agatgtgaca cggtcaccaa ctttaaaatc tttaacgtct gctccaactt
     7861 caacgatttc accagaaaat tcatgaccta atgtcactgg aaaattaact ttataatgac
     7921 cttcataagt atgaatatct gtgccacaaa ttcctgcata atgtacttta atctttactt
     7981 tatcatctag cggtgttgca acttctttat caagaagttc taagttgcca tgtccttctc
     8041 ttgtttttac taaagctttc accacaaaca cctcgatttt taattgaata gactaaatag
     8101 tttaaagata agatagttaa cgatattacc accttgatca atacttgaaa tttcagatga
     8161 accttttggc atttgtacat tcgtaccttt cgccatatct gtgaaaatgg gtgctacgtc
     8221 tgttgcaata tatagtgaaa ttgcaatcat aatcgtaccc acaatgacag aatgaataat
     8281 gtttcctctt gctgcaccaa caataaacgc gacaacaaat ggtatcgttg ctaagtcacc
     8341 aaaaggtagt acttggtttc ctggtaaaat aacggctaat aaaacagtga taggtactaa
     8401 aattaatgct gtcgaaataa ctgctggatg acctaatgct acagccgcat ccaatccaat
     8461 ataaatttca cgttcgccaa aacgtttatt tagccatgtt cttgcagact ctgaaactgg
     8521 cattaaacct tccattaaga tttttaccat tctaggcatt aataccatta ctgcagccat
     8581 tgacattcct aaattaatga tgtctccagg tttgtaacct gctaacacac caatacctaa
     8641 acctaaaatc aagccgacaa atatagactc tccaaatgcg ccaaaacgtt tttgaattgt
     8701 ttcaggatca gcatctaact tattcagacc gggtactttt tgtaacaatt taactaagta
     8761 aatacctggt gcataagaaa ttgtacttcc tgtagcaatc gatacacctg gtaaatcaaa
     8821 gaactcactc atcatcggtg ctgtccaatc tgctactttc aaacagataa tttggaaaat
     8881 aactgctgct aataacgctt gccaaatact gcctgatacg gcataaacca ttgctgctgt
     8941 aaacgtataa tgccaaaaat tccaaatatc tacattcatc gtctttgtca ctttagttac
     9001 tagcaataca acgttaacta tgattccgag tggaataata aatgctgcga cagatgatgc
     9061 ccaagcgata gatgatgttg ctggccaacc tacatcaatc acattcagac tgacgcctaa
     9121 atttttaacc atcgcttgtg ctgctggccc taaattttta actaataaat cgatgactaa
     9181 gaaaatccct acaaaagcca cacctattgt taaaccagac ctaaatgccg ctccaatttt
     9241 ctgcctaaag aataggccaa gcaagaatat gacaaccggt aaaataacag ttgcacctaa
     9301 atctaaaaat ccccttacaa aatcagtgaa gtaactcata tttaaaccct ccctgttata
     9361 tatgcattgt cacgatactt tccgattgtg attacatttg acgttacagt catttcaacg
     9421 acaacccttg ctaaattcga ctgcagtcct tttgaattac agtcactgcg tttctatgtc
     9481 atcaacaatc atttgtcgtg atagtcattt atatgcaatt tgcatatatt aatatgttat
     9541 cgacccacgt tacatatcaa ttccgttatt tttgtaactc tgttaagatt tgttgttttg
     9601 tttcttcaat accaatacca gttaagaaat tacgtgcgtt gataactggg aatttatatt
     9661 ctttttttgt cattgcagtt gtaactaata aatctgcagt gtcttcataa ggtccaactt
     9721 ctgtaatttt gatttgttta atatctactt taatattgtg ttcctttgcc atttcttcaa
     9781 ttgcattatt tactactgtt gacgttgcaa tacctgcacc acacgctact aatacttgtt
     9841 tcattttcaa ttcctccaat taatttttag ttatattcca aataatcatt gattagtgtt
     9901 gctaaaattg tttcatcttt cgttcgtaga atctgctcca atttttcttc actttgaaaa
     9961 atttgcatca actgttgtaa cagcttaagt tgatcatcta ctttatccat tgctaacata
    10021 aaaacgattt tcacttctgt ctgttgatca agtgttccca tttcaataaa cggcacttct
    10081 ttttctagaa cagccacacc tatcgttcta tggttaatat gttcgacatc tgtatgcggt
    10141 atagcgaccg aacatagatg cgttggtaaa ccagtagcaa attctttttc tctgtcgatg
    10201 actgcatctt taaacgttga cttcacgaac ccattttgaa ataacacatc tgacatttgt
    10261 gacaatacgg attctttatc agttgccgac aaattgagca ttatattttc tttatgcact
    10321 aattgctgtc ccatccattt tccctcgctt ctttatttga ataatttttt aaaatctcat
    10381 ttacatcaga atttttgcga ctttgtatga tgcgcttaat tgcgtcattg tcttgcgcca
    10441 catctctcaa ttgtagtaac gctcttaagt gtgtcacttt atcaacagca gcaataggta
    10501 caataatatg gattgctgtg ccatctgaca tgtatattgg ttcttgtaat atcaacatac
    10561 tcatcgctgt tttatgtaca tgcttttcag agtatgcatg tggaatagca atattttgca
    10621 tgataaccat atacgaatca tcaaatacct gttgcatctc atgaatatat ttaatatcaa
    10681 taaaatgatt agcaactaac acatcacttg ctttagcaat agcttcatca atattttcaa
    10741 catgatgcat tcttttcacg tgccttgccg gtatcaagtc agctaaatct aatgtcttat
    10801 tttgtgtgac aatcgatcca ttaatggttg aaattgaatt ataattggca ataaaatctt
    10861 ctaaaccatc acgtagttct gtaatgtcat taactgtcgt tgtgcgttca attaatgcca
    10921 ttaacttgtt tatttcctta tcaatgtcag ccgattcctt attaatgtac ttcatcactt
    10981 ctttacgtaa ctttcgttgc tcattttcag ataaagctac ttttgtgata aataattttt
    11041 tatgtgttag gacaaacatt ggtgaaaaga cgatgtcata atctaatgtg taattttcaa
    11101 atgttctaag tgaaatcgca tctaagaaaa taatttctgg aaataagttt cgcaactcgt
    11161 ataacatcat ttgtgatact gacgtgcctt gtgtacacac gataatagct tttatcttgc
    11221 catcgaagtt ttcatcttga cgtctcaaac tacctccgaa caacatggtt aaatatgcta
    11281 tttcattatc aggcaacgat tttccgaaat attcagttaa cgattgacat gattgtttca
    11341 ccatatgaaa taaggattga taatttcctt gtaaaggatt tattaattca tcacgatccg
    11401 ttaagttata tttaatccta taaaaagcag gcgttaaatg taacaagagt tgctgtgata
    11461 atttctcctt atcttcaatg ttaataaaag tgatttgttc aaaatggtga atcatttgag
    11521 cgatggccat cgttaaattc gatatgctat ctgattcttg caaatcagtc cattgcacac
    11581 ttgttgaaag taagtgtaat gtcaaatata acttttccgc ttctggcaaa tccggctcat
    11641 gttgcgtcat aatctccgtt gcttgatatt ctttcgtatc cctcaaatac tgataattaa
    11701 tatttaatgg attcatcaca tgaccacttt gaattcgtct acgaatcaca caaaggacat
    11761 aaggcaatga actaagtgat ttgtctataa agcgactctt caaaaattgt tctacctgtt
    11821 tgatcttgtc tttttgatat gcgatatctt cgaatgttaa gttgagcgcc tttaaaactt
    11881 cacttttagt aatatcatga ttcaaccttt gatcaatcaa cttaatgaag aaacggcgaa
    11941 cttcaaattc atcaccaaca atttcataac catgttttcg agaatactta agtgacaaac
    12001 catgattttc caattgctct ttcacatgat ttatatcgtg aatgacagta tttttactga
    12061 cttgtaaatc aattgaaaaa tggtttagag acattgcgtt ttccttacta aaaagcatga
    12121 gcattaaata ataacgacgt gtttctatgc taaaaatgac attgttgccg tttaacattt
    12181 gctgctccga tacatctcgc ttgaataacg tcatgatttc agaacttaca ataaaatttc
    12241 cttggcttgt tctttcaagt tttggataac cctcttgttc aagccacaaa ttgatttttt
    12301 gaatgcgata tcctagttgt ctacgagaca aaccaaatat cgattcaagt tctttaccat
    12361 gaatagtagg attcaataca atttctctga gtatttgctg attatccccg ttcatgttcg
    12421 attctccttt gagcatctga gctcaattta tcatcaatcg tcacaccgat gtacacactt
    12481 tgaacacata ttttcaaaat gagcatgtac atcattgtga tgttttaaca acatttcaat
    12541 tatatctata ttttttgtga ttttaatctt ttaaaataaa gcaattgaaa tttttgcata
    12601 tatttttgtg ttttgtgttt ttttgaagca tttttaacat acatatctca atcattatca
    12661 aattgtcatg accattgtaa cccaatacaa aaaccctaag gacgcttata tcaggcgcct
    12721 tagggttaac tgtatctatt taattaagta ttattattcg tatgtacgta acttatggtc
    12781 tatcaagttc cacacttctt caacatcaac tgctgtagca aaataagcat tggcaggctt
    12841 acctgtaaca tgatttaaat cgacagccat agtgccataa gttagtggac tttgatgttc
    12901 aatgtcgata ttaacgggta ccattgtaaa caattctggt tgtaacaaat acaaaattgt
    12961 acaagcatca tgtattggac caccatccat attaaagtga gtcttgtatg tcttcttaaa
    13021 gaattgcaat aattctacga cgaactgtgc aacaggatta ttgatacttt caaagcgttc
    13081 aatcacgtga tcgtcggcta aaacttgatg tgttacatct aaaccaaaca catttatagt
    13141 aatcccactt tcaaaaacac gcttcgctgc ttcagcatct acccaaatat tgaattctgc
    13201 tgtaggcgtc caatttccaa atgtaccacc acccatcaaa gtaatagatt caatatgctc
    13261 agcgattctt ggctcacgaa tcaatgccgt tgctacattc gtaagaggac ctgtcgctac
    13321 aattgttaca ggtgtatcac tcgtcatcac tttgtttata atcacatctg atgctggcat
    13381 tgcaactgct tgacgtgatg gtgtcgacgg tagtttcgga ccatctaatc cagattcccc
    13441 atgtatttca gaagcaaagg cagctggttt aattaacggc ctatccgcac ctttcgctac
    13501 tgctatatct tggcgtccca taatatccaa tacgttcaag gcgtttgtcg tattcttgtc
    13561 aactgattga ttacctgcga ctgttgttac agctaatatc tctagtggac tgtcaattgc
    13621 ccccgctaaa attaatgcta ttgcatcatc gtgtcctgga tcacaatcca taataatctt
    13681 tcttttcatt tatatatcca cctttcttaa gttgttatcg atagcttatg tatatttatt
    13741 tatgtggtga atcatgttta ttttgaaaaa tagttttaac tttctcatat ttttggatac
    13801 aaacactatt tatctatttt atggcttata aatttatccg atatgcctta tcaacctacc
    13861 tcgctaaaaa taggatgtct acatatctat accgactttt gtcaactcat tttcacaaca
    13921 atataaacag caatttatat gattgttaca tgattcaaac aatttttatg aaaaatattt
    13981 tcatacacag aatatatatt gatattaaat ttctcaaaag ctatattgag aataattagg
    14041 agggatgttg atgaaatctt tatttgaaaa agcacagcag ttcggcaagt cctttatgtt
    14101 acctatcgca atcttaccag ctgcaggtct attgttgggt atcggtggtg cattaagtaa
    14161 tccaaacacc gttaaagcat accctatttt agatattacc ttattacaaa atatttttac
    14221 attaatgtca gctgcaggta gtattgtttt ccaaaattta ccggtcatct ttgcaattgg
    14281 tgtcgcaatc ggattatcta gaagcgataa aggtactgca ggtttagctg cgctgctcgg
    14341 tttcttaatt atgaacgcaa ctatgaatgg cttattaact atcacgggca cattggcaaa
    14401 agatcagctt gcacaaaatg gacaaggcat ggtgctcggt atacaaacgg ttgaaaccgg
    14461 tgtttttggc gggattatca caggtattat gaccgcaata cttcacaaca aatatcacaa
    14521 agtggtatta ccaccgtatt taggtttctt tggtggctct agatttgtcc ctattgtcac
    14581 agcatttgcc gcaatctttt taggtgtatt gatgtttttc atttggccaa gcatacaagc
    14641 cggcatttat catgttggtg gatttgtaac gaaaacaggt gccatcggta cttttgttta
    14701 tggcttcatc ttaagattgt taggtccact cggtttacac catatttttt acttaccgtt
    14761 ttggcagacg gcacttggtg gtactttaga agtcaaaggg cacttagttc aaggtacgca
    14821 gaacatcttc tttgctcaac ttggtgatcc agatgtgacg aagtattatt caggtgtgtc
    14881 acgctttatg tcaggccgtt ttattacgat gatgttcggc ttatgtggtg ccgcacttgc
    14941 aatttatcac acagctaaac ctgaacataa aaaagttgtc ggcggtttaa tgttatccgc
    15001 tgcactcact tcatttttaa caggtattac cgaaccttta gagtttagtt tcttgtttgt
    15061 cgcacctatt ctttatgtaa tccatgcctt ctttgatgga ttagcattta tgatggcaga
    15121 cattttcaac attacaattg gtcaaacctt cagtggaggc tttatcgatt tcttactctt
    15181 tggtgtgcta caaggtaata gtaaaacaaa ctacctatac gtcataccta ttggaattgt
    15241 gtggttctgt ttgtattaca tcgttttcag attcttaatt acgaaattta atttcaaaac
    15301 acctggtcga gaagataaag ctgcagcaca acaagttgag gctactgaaa gagcacaaac
    15361 tattgttgct ggtttgggag gcaaagataa cattgaaatc gttgactgtt gtgcaacgag
    15421 actacgcgtc acacttcatc aaaatgacaa agtcgataaa gtattactcg aaagtactgg
    15481 tgccaaaggt gtaatccagc aaggcactgg tgtgcaagta atttatgggc ctcacgttac
    15541 agttatcaaa aatgaaattg aagaattgct cggggattaa gactaaccga aatatcaaca
    15601 gaactaatgg caacgatgta cgaagtaaga agtgacatcg ttgcttttat ttttaatgtt
    15661 acatttgaag cattaagttc atcatgcact gtagtgagcc cgcaaatcgc ctctgctaga
    15721 caatcatctt aatgctatga ttaaagctta agtgccagat ttgaatttaa tttcaacaac
    15781 gactttcact acattaaaaa tagggctact cgacacatat agttgtatca aatagccctt
    15841 tatacaattt tttgggtaag gttttacaat ttttgggatg gtatagattt tataaaaagt
    15901 tatttaagtt cttctgcttc agccataata tcttttaatg ttttagctga atgtgcgaac
    15961 ttgctttgtt cttcgtcgtt taatgggatt tctacgacgt tgcgaatacc gtttctattg
    16021 atgactgctg gaacaccaat ataaacatct tcttcctcat attcgccttc taataatgct
    16081 gatacagtca atacggcatc ttcatttctg aaaatcgctt cagtaattct agctaatccc
    16141 attgcaacac cataataagt ggcaccttta gcttgaataa tgtcatatgc tgcatcacgt
    16201 gtttgaacaa aaatttgttc aatttgcgct ttgccctcag gacgttgttc aagtaatgtc
    16261 ttcaaaggtt gacccgcaat attagcgtgt gaccatactg gtaattcagt gtcaccatgt
    16321 tcaccaataa tttgagcatc gacgctacgt ggcgcaacat cgaacgcttc gcttaacaat
    16381 aatctaaagc gtgcagagtc taaaattgta ccagaaccta taacacgttc tttaggtaaa
    16441 ccagagaatt tccatgttgc atacgctaaa atatcaacag gatttgtagc taccaagaaa
    16501 ataccatcaa attttgatgc cattacttca ccaacaattg atttgaatat tttcaagttt
    16561 ttagatacta aatctaaacg tgtttctcca ggtttttgtg cagcaccagc acagatgaca
    16621 actagatccg catcatgaca atcactgtat tcgccagctt tcacacgaac tgttgttgga
    16681 gaatatggtg tggcatgttt taaatccata acatctcctc gaactttttc agtgtctaaa
    16741 tcaatgatga ctaattcatc aacaatgctt tggttcacta atgaaaatgc gtagcttgaa
    16801 cctactgcac cattacctat taatacaact ttgttccctt taaatttgtt cattacaaaa
    16861 actcccttat gattaattca ctaacataca tgtagcttca aatatgttag tttaatgctg
    16921 cttattgacg atacaaaagc aaataaacat ctcttttatt ttcaacgcat aacttaaaag
    16981 gtcatgtgtc atccgctttt aagtttgtga tttatttcac atataaaatg taacatgcat
    17041 taagtactgg gtcaatatta aattgtgatt tatttcacat tttattttaa tttttacacc
    17101 tttttaattt gtatacgatt acatcttaga tgtctttagt cttcgtactt cgccagtgat
    17161 tatttacact ttcacatttt tattatcatg tttacttttt tctaggaaaa caacaatgtt
    17221 ttttgaatta gtcaaataaa tgcgctcaat cgtcggtgtg caaacagaca attgtacaca
    17281 atgcttattg ataagtattt aaaaaattaa aaatgtcata caattatcaa atttgccatt
    17341 ttatttatat tttctcaaac caattaattg aatatcgaaa tttttagtag aataatcaaa
    17401 atatacagat taaaggagga gtatcatgct tacagaacaa gagaaagaca ttatcaaaca
    17461 aacggtgcct ttacttaaag agaaagggac agaaattacg tcaatctttt atccaaaaat
    17521 gtttaaagcg catcctgaac ttttaaacat gtttaatcaa acgaaccaaa aacgaggcat
    17581 gcaatcttca gcattagcac aagctgtaat ggccgcagcg gttaatatcg ataacttaag
    17641 tgttattaaa ccagtcatta tgccagtcgc atataaacac tgcgcactac aagtttatgc
    17701 tgaacattat ccaattgtgg ggaaaaattt attaaaagcc attcaagacg tgacaggatt
    17761 agaagaaaat gaccctgtca ttcaagcttg ggcaaaagca tatggcgtta ttgcggatgt
    17821 gttcatccaa attgaaaaag aaatttatga tcaaatgatg tggattggtt ttaaaccatt
    17881 caaaattacc aatattaaac aagaatctga agacattaaa tcatttacag ttgaaactga
    17941 agaatatgac tttagtgaat ttacaccagg ccaatacatc acagttgatg tttctagtga
    18001 taaacttcca tatagagcta aacgtcacta ttctatcgta tcaggtgaaa aaaaccattt
    18061 aacttttggc gttaaacgtg atgtcacaac agaacatgaa ggcgaagttt caacaatttt
    18121 acacgatgag attaaagaag gcgatatgat taatttagct gcgcctgtag gtggattcgt
    18181 attagagaat acgactgaac cacaactttt cttaggttca ggtattggtg ttacaccttt
    18241 agtagctatg tatgaagctg cctctgccaa aggtttagat acacagatgg ttcaagttgc
    18301 tgaaaatgaa caacatttac ctttcaaaga caacttcaac agtatcgcaa gccatcatga
    18361 caacgctaaa ttatatacac acttaaaaga taaacaaggc tatattggtg ctgaagaatt
    18421 acaagtattt ttagcaaata aacctgaaat ttatatctgt ggtggtacaa aattcttaca
    18481 atctatgatc gaagcactta aatctttaaa ttacgatatg gatcgcgtac actacgaaac
    18541 atttattcca agactaagcg ttgcagtata actgagaaaa gaggtttagt gctcatatga
    18601 aacttaatca acgttacgta aaagtatttg cattatattt cgtaagtatt gttactgcaa
    18661 atattattgt taaaaataat aatttaatta aaacattgat acaaaccata gccgggtaca
    18721 cggtctttgc agttggtttg aagtatttaa ctaaacgtaa aaataaatga catctatacc
    18781 aaaacagcta tgactttact ttgtcatggc tgttttttaa ttgggagtag gacagagatg
    18841 atattttcgc aaaatttatt tggtcgtccc accacaacat gcattgatgt atgctcactg
    18901 aatttcataa gaaaggagtt cacaagatga ccgtagatat tggacggatt tatgacaata
    18961 aagataatac cgacgctatt cgtatcctag tcgatagagt ctggccgaga ggtatttcga
    19021 aaagaactgc taacctagat tattggttaa aagacattgc cccttctact gagttgcgac
    19081 aatggttcca acatgatcct aaactttttg gagcttttaa agaaaaatat gaaaaagaat
    19141 tacgtgatca ggatgcgcaa aaagatgctt ttgaaaaatt aaaggatatt gtaaatcagc
    19201 ataatcatgt tctattgtta tatgcagcaa aagatactaa acataaccaa gctgtagtac
    19261 tacagcagtt gctcaatact tagttatata tgtttatcgt gaatcactat aaatgtcgct
    19321 aacttcattt ctaatattta tttttaaaaa gcataattac tacaattaat tgaactttaa
    19381 taattactaa acttgaacaa cattttactt taacaaaata aagttttaaa atattaattg
    19441 ttgggtttta catagtaatt agaaagagag tgttaatgaa atgaagttta aaagactagc
    19501 aactatattt tcagcagtat tagtgttatc aggatgtggc tcaatgcatt catctggcaa
    19561 agacttaaat atttcgttac cgttaaaaac aaagtctatt gcaccttatg aaacagatgt
    19621 accagttaag attggtgctg cagaatcatt atttaaaaca aatgatcaag gaaaaataga
    19681 aaaagcatta gtgaaatcgt atcatcaacc aaatgataca acgttagata tagaattaaa
    19741 agataatatt aaatttcaaa acggtcaaaa attgactgca gaaaaagtga aatctagctt
    19801 tgaaaatagc atgaaaaaaa gcgacttggt caaatattca ttaccaatat catcaattac
    19861 cgctaaaggt caaaaactga caattaaaac caactccgct taccctgaac ttgtatctga
    19921 attagctaat ccttttatgg caatttatga tacagatgct aaatcagatg ttaatcaaac
    19981 tcctgttggt acaggccctt accaaataaa agattataag caatctcgaa aaatatcatt
    20041 gtcgaatttt aaggactatt ggcaaggtaa accgaaactt gatcatatta ctgtgacgta
    20101 ccaagaagac ggcaataatc gcgtcagaaa tttagaatct caaaaagatg atttaataac
    20161 tgatgtccca gttaataaag ttcaagacat agaaaataat caaaatttaa aagtgtcaaa
    20221 agaatctgga tttagaactt ctttacttat gtataatcat actaataaaa aaatgactaa
    20281 atccgttcgt gaagcattag atcatatcat tgatagacaa ggtattgcag atcatattta
    20341 tcaaggttat gcgaaacctg caacgagtcc atttaatgat aaaattccat atattaaaga
    20401 acctaagtta accaaacaaa atatcgaaca agcaaaaatg ttattagcta aagatggtta
    20461 tacaaaagaa caccctttaa aaattaagtt aattacgtat gacggtcgtc cagagctatc
    20521 gaaaattgcg caagtattac aatcagatgc taaaaaagca aatattgaaa tcgacattaa
    20581 aagtgttgat gacatagaag gttacttaaa agaccgttct gcatgggatg caacgatgta
    20641 tagtttcgga acaattcctc gtggagatac aggttatttc tttaatcaag catataaaaa
    20701 agatggtgct attaataaag gagactacaa taatagcaac gtcgatgatt taattaatca
    20761 attgaatcat actgtagatg ttaaggaacg tcacaatatt tctaatgata ttattaaatt
    20821 atcaagtcga gatgtaccaa acagttatat tgcttacaac gatcaaatag tcgctgctaa
    20881 ctctaaggtt aaaaattata aagtaacacc tgaaggtatc tatttaatcg attatcgaac
    20941 aacgattgaa cgataaggga gtgtgggaca ttgatttatg tctcagccct gcttcaacta
    21001 taaaaaccac gccgaagtca tccaatatga ttatggcgtg gtttaatttg cttatatgat
    21061 tgtattaatt tctgtttgat gaatttcaat aaaatgatta ttacatactt tcagtgaatc
    21121 atgttcattt tatataaatc taattgtcac tatttgttta acgtgaaaca cttttttaac
    21181 tccaaaaagt attcctattc cactctttta aagacaaatt ataaaaatag cttatgtcac
    21241 aaacatatga gcaacataag ctttaattat tattcgtcta cttttttctg ttgttcttta
    21301 aattctttct gcagtaaatt cacttgtttt aaacctgccc ccattaaaat gaatacaagt
    21361 atccacacaa taacgattaa aataaatatt ttcaacgtat cactgccaag tacagttaaa
    21421 gatgtatccc aaatgccatg taaaacaacg gctgataaaa agaatattaa aaagcgttta
    21481 tcaaaaatat ctttgaattc aaagccatgc tgttctttcg caataactat tgcagcacca
    21541 acaatcgctg accaaactaa atgaccacca atcgcagtcc acgcacgtgt gaagacaata
    21601 tctaataatg ggacattttc tcctaaagcg aaattcaaaa tataacctgc tgattcaaaa
    21661 actgcgaacc ctgcaccaat agcagcaccg attaataatc cattcaaaat cttatttgtt
    21721 ttcaatttat tgacgaaata aacaataatg agtgcttttc cagtttcttc aactaatcct
    21781 actaaaaatg catcgaaaaa tgttaaagaa ccaaaccttt cgaattgatc actaaaaacg
    21841 acaaatctat ataataccat cgtacttagt aatgaaaata cgccgccaat aaagaacatg
    21901 ataataactt caaaaatgct aatattttta aacgcatttg attcataaaa gaagaacaaa
    21961 cccgataatg gtactgttaa agcccctata aaaatgagac ccggaatcgc gttagtgtta
    22021 ttaaaaattg ctgccatgac ccataatcca ataaatgtta ctgtgaaagc gatgaatact
    22081 cgagaaaaga gccatggctt cccccattct tctgatatgt cacaaattgc tggcgtagtt
    22141 ttagcagtac ccgctataaa tatttcatct gcttcgtttt tagtatgcgg cttaaatact
    22201 tcagaaagca tatcttttaa attaagattt aagctttcat cattgcccgc tagtctaccg
    22261 attgatttgg ttgcattatc aaataaactt cttgcttcgt tttttaaatc tgtattttgc
    22321 gctgtttcat gcgctttagc ttccatttgt ttatcgatag attcattatg tatgtcggac
    22381 gtcttagttg attgtgtact tgctgatgac atcccctctt cacctgtatt aggtgcaact
    22441 gattgcgttt gtgttgtctc ttctttttct gtatttcttc gatgattaag accttccata
    22501 gacttaccta ctttcacttt tcataataaa actattttaa tttaatattg atggattttc
    22561 catattttaa tataaatact ttgttcgatt gggcactttg tatttcatat ttaaaaatca
    22621 aaacctattt aactttttat attccgaaca atatttttat ataagtattt taatgatatt
    22681 caattcatta caaacccttg atattacaat tgttatcgac cgtcaaaatg tgtctatact
    22741 gtgtctatta tcagaataaa aagatatttc taattgattt taaacgtcgt tatgttatat
    22801 ttcttgtaag ggtttacaca aagtgtaaaa acgctatatt ttatcaaatg tgatggaggt
    22861 gacttgcttg aaacaaatca catggcacga cttacaacat atcattaaag atggtgatgt
    22921 gattggttta ccagcattag ctgtagccaa cttacccgcc gaagttctac gtgctgtgtt
    22981 agcgcaacat gacacatatc atacgcccaa tgatttaacg tttatattag cgaatgatat
    23041 tcatagttta ggtgccgcac cggatttaga tgattttata gaacgtcgca tgattaaacg
    23101 tgtcattatg agtattttaa cggcttcttc caaaacggca caagcaatga aaaataatga
    23161 cattgaagct tattttttac cacaaggtat cattgcaact cattatcgtc agagtaatca
    23221 attattacct ggagttatta ctaaaatcgg attaaacaca gctgttgatc ctagatacgg
    23281 tggcggtaaa gtaaatacac gaacaacgga tgatttagtt tcattagtaa ccatcaacga
    23341 tgaaacatac ttacattaca cattccctag cgttgatgtg gcactactga gaggaacata
    23401 cgcagatcaa caaggtaaca tttatttaac tcaagaagcg tacttgagcg agtgttatca
    23461 tgtcgcatta aacgcgaaag ccaatcatgg gaaagttatt gtacaagtta aagctttagt
    23521 tgacgactat caactaaaac cgaatgaagt tgttatccca ggaaatcttg tcgattatgt
    23581 atacgtcaca gaagatgaaa agaatcaccg ccaagtaatt caaagtcatt atttaccagc
    23641 cttgtctgga gaagaacgaa ttgatggaat acctgaaccc gcattacctt ttaatagtcg
    23701 caaattgatt ctccgacgtg ctgctcagtt tttaacttat ggcgatacaa ttagcatcgg
    23761 ttatggcatc aataatgaac tctctaattt attgcatgaa gaatgtgttg aacatgatgt
    23821 gcaaccgatt ttagatgttg gcattttcgg tggattcgtt gggagtcgtg aacattttgg
    23881 tatgaattac aatgcagatg tgcgcatgcc tcatgatcga gcatgggatt ttatttataa
    23941 caatggtgta tcagttgcct atcttagctt tgctgaggtt gatcaatacg gcaatgtcaa
    24001 cgtgtcttac ttcaatgacc gactaaatgg atgtggtggc tttatagaca ttacgcaatc
    24061 tgtaaataaa attatctttt caggtacttt tgtagctggc agtcatgtct catgccataa
    24121 tcaacgatta aacattgaaa ctgaaggaca aaaccagaaa tttgtatcag atgtgagcca
    24181 tatcgacttt aatgcacaat attcacaatc actcgagcaa gaaatctatt ttgttactga
    24241 tcgtgcagta ttcgaactca ccaatcaagg cttgaaacta attgaaattg caccaggtct
    24301 tgatttgcat aaagatattt tgaatcaaat ggcttttaaa ccaattattg ctgatcattt
    24361 aaaattaatt gataccagca tttacaaaga aaaatgggga caacttaaac aatcaattca
    24421 taaagtatga aagtgaagct aaagggggta tactaatgaa tttcgattgg ataaaaacgc
    24481 gttcagactt cgatgatgac aagcctgccg ttattgatca cgcaaaacaa acatcttgga
    24541 cataccaaca actcaatgca cgcgctgata atatggcaca ttatttaaca tctcaaggtg
    24601 ttaaaaaagg cgatgttatc ggtatttttg cgccaaatga tattgcaata ttagatttat
    24661 tgtttgcttg ttttaaaaca ggtgcggttt ttttaccatt gaattggcgg cttaacccaa
    24721 aagaaattgc agccattgtg gaagatgcac aattaaaact gctcttctat gctgaaaaac
    24781 atttaagttc actcaccgat attgaccaaa acttattgca tatggatatt gatgtggcgc
    24841 aatatgatga aatcgtaaat ccagattatc accaaccttt tcaagcaaca cctgttgaac
    24901 cacaagatct tgcagcatta atttatacaa gtggtacaac cggatcacct aaaggtgtga
    24961 tgttctcata tgaatcattt gttcataacg gtgcaaactt agagctgacg tataagttca
    25021 attcaaacta tattacgatt gtatcaacac caatgttcca tgttttaggg tttaacgata
    25081 ctgtattacc agtattaatg tcaggtggca cacttattct tcaacgttac tttaacggcg
    25141 aagaattgaa tgacatgatt gcacaatatc accctacatt tattatcatg attccgacaa
    25201 tgtattatag tacgctacgt gctagtaatt ttaatcctga aaattttaga gctatggatt
    25261 atatcatcca aggtggttca caaccattac caagtatcca agcagctttt aaacaatatg
    25321 gcataaacat tattaacggc tatggtttaa ctgaagcacc tcttgtactt gttaatacac
    25381 cggaaaattc aaaacgtaag ccaatgagta ttggtaaagc agtcatgttc gttgatgcac
    25441 gtatccttga tgataacggt gaggaagtac ctactggtga gattggcgaa cttgcaatta
    25501 aggctaaaaa tgtcacgcca ggatattgga ataaaccagc agagactgcc aaagcatttc
    25561 atggtcgata tttattaact ggtgacttag cgaagatgga caacgatggc gatatattta
    25621 ttattgaccg caaaaaagaa ttaatcataa ctggtggcga aaatgtctta ccatccgaag
    25681 tcgaaaatgc tttagctgag catccactag tagaccggtg tgtggtcgtt ggctatgatc
    25741 atccaaaata tggtgaatca attgctgcag ccattatact tcgcgaagat gaacctcatt
    25801 acgctgaaat tttaaatcaa catatgcgaa gtcgtttagc aggttataaa gtcccaagaa
    25861 tgtatgtacc agtgacacat atgccgttaa acagtacgca gaaaccagat aaacttgcga
    25921 ttcgacaaat gatgaatgac aaagtctcgc aaacacttta aaggtgataa aaatttttga
    25981 catttagtgt aagcgtttac aaataaagcg tgttgttttt gaattaaatg catttcacat
    26041 tagtattcat attattttta ggaggaattt atatgacatt tgaaaaagaa acggtcttaa
    26101 aaacattatt tcctgaagat gtacttagta ttgctaaagg tttaacagac ggtgaagtcg
    26161 aatttttaca acaagtagat tcattgctag aaagtaagta ccgtgaaaat attaatcaac
    26221 attggataga cgctactgta cccgaggact attttaaaga tctgggagaa ttaaattatt
    26281 ttaacaatcc attactttac aaggatcgtc caaacgccaa aatgcctagt caactatttc
    26341 agtttttcat gtcttaccta ctcgcgcgat ttgatatttc cttagctacc ctactcggtg
    26401 ttcaccaagg tttagggcat aacactttct atttcggagg tagcaaagaa caaattgcga
    26461 aatatgtacc taaattacag tcacatgaac tgcgtacatg ctttgcttta actgaaccag
    26521 aacacggttc ggacgttgcg ggaggtcttg aaacagtcgc tgaacgccaa ggcgatactt
    26581 gggttatcaa tggtgaaaag aaatggattg gtggtgcaca tgtatctgat gtcattccag
    26641 tattcgcagt aaataaagaa actggcaaac cccattgctt tgtagtcaga ccagaacaag
    26701 atggcgtcga tattgaagtc attgataata aaatcgcact tcgcattgtt cctaacgccc
    26761 taattaaatt aactaatgtc aaagtagatg aagcggatcg cttacaaaac ataacaagct
    26821 ttaaagatat tgccaaaatt ctttattcaa cgagagcagg cgttgcttat atggctacag
    26881 gtggtatggc tggcgcttta cgtgccacat tagattatgt cactgagcgt aagcaattcg
    26941 gcaaaccaat tagtaaatat cagttaatac aagaaaagct agcaatgatg caaggtaatt
    27001 tagctcaagc aatggcaaca tgtgctcaat tagctaatat gcaagcacat ggtgaatatg
    27061 acgaggttgc aacttcaacg gcgaagatga tgaatgcctt acgtttgcgt gagacagtag
    27121 ctatgggccg cggtattaca ggtggtaatg gcatactagc tgacgattat gatattgcac
    27181 gtttcttctc tgatgcagaa gcgatttaca cgtacgaagg tacacatgaa attaatgcct
    27241 tagtaattgg acgcgctttg actggagatt ctgctttcgt ataaatagca aataattata
    27301 tgagatgcat taatttcact aaaaaagact tattttaagc ataaagcttt ttccttaaat
    27361 aagaggctaa gatgactgtc aaagatactt aattaatttt ataaaatagc aacgttattc
    27421 caattatctt aatggttatc ttatcctcaa ctaaattgga ggaatcacta tgacaattaa
    27481 taaagtaacc gttcttggcg caggcacaat gggcgctcaa ctggcagcac tttttgtgaa
    27541 tgctggactt aaagtaaaac tattagatat tgtagtggac aaaaacgatc caaatctcat
    27601 tgcgaaaaaa tcttacgata aaattacaga taagaaacgg ccgctactat tcgacttaaa
    27661 tctagcgagt catttaacat atggtaattt tgatgatgac ttggtaaatg atgatgctga
    27721 tttatatatc gaagcagtca aagaagatat tgaaattaag catgctgttt ggcaacaagt
    27781 tctacaacat gctaaagaag atgctttatt cgctacaaat acatcaggta ttccaattaa
    27841 tgcgattgct caagcattta acgagaagga tcaagaacga ttctttggtc tacatttctt
    27901 taacccacca cgtattatga aattagtgga gttaatacct acgtcacaca cgaaggaatc
    27961 tattatatta gatgtaaaaa atttcgcgca aaatgtgtta ggtaaaggtg tcattgtcgt
    28021 caatgatgtg cctggctttg tcgcaaatag agtcggcacg caaacaatga atgatattat
    28081 gtatcgcgcc gagcaacaca agataagcat tgtagatgtg gatgctttaa ctgggcaagc
    28141 gattggtcgt cctaaaacag gtacatatgc gctatctgac ctagtcggtt tagatattgc
    28201 agtgtctgta attaaaggca tgcaacaagt acctgaagaa acaccttatt ttcatgatgt
    28261 caaaattgta aatacgttgt ttgacaatgg cgcactcgga cgtaaaacga aacaaggatt
    28321 ttacaaaaag gataaagaaa ctaaagctcg acttgtttac gatgttgaaa aacaagatta
    28381 tgtacctgta tcgcaaccac aattaccaat tttaaatgaa tttaataaag acttagtgca
    28441 taaccttgat accatattca atgcgcaaga cgaagcggga ctatttttat gggagacatt
    28501 acgtaataat ttctattact ctgctatcaa tgtacctaaa gctaccgatg atttccgaga
    28561 catagaccgt gcgcttgtct gggggttcaa ctggaaactt ggtccattcc aattatggga
    28621 tgcaatggga tacgaacgtg ttaaaacacg tatggaagac gaacttggag acttaccaca
    28681 atggattagt gatttagatg gtggctttta taaacaagat gagaccattg aatatgcaac
    28741 acctatttct cacttcgtaa aagatgaact ttgggataaa ggtgatgcca aactttccgt
    28801 aactcatgat gatcaactgt tactgaaatt acaaagtaaa aataatgtca ttaccgatga
    28861 attcaacgat gcgttagttg atgcgattga tttactggaa aatgaccatt acacaagtat
    28921 ggttatttat gcagatggta acaatttcag tgtgggtgct aaccttttct taatgaaaaa
    28981 ggcgcatgaa gacggtcttg tagatgatgt cgttgcacaa tcaattgata aattacatta
    29041 tagctttaat cgtttgaagt atagtttgaa accagtagtc acagctgttc aaggtcgtgc
    29101 cttaggcggt ggctgtgagc ttgtacttta ctcacctatt gttgtcgctg caagtgaaac
    29161 atatatcggt cttgttgaag caggtgttgg cttattaccg agtggcggtg gccttgcaga
    29221 aatggctgat cgcatattac gcacatcgca taagtttgat gacaaacaag cttccatgac
    29281 aaaagtactg acgaatatcg catttgcgaa agtctctaca aatgcctttg aggcacgtcg
    29341 ttatggttat ttacgtgata cagatacgat tattttcaat acagcacaac gtgtcgaagt
    29401 tgcgctcaaa cgtgcgaaat atgaagcaga aacaaactat attccgaatc ctagacatca
    29461 atatatcgct ttaggtgaag acttcaaagc attgatccaa ggacaattag atgcgcaaag
    29521 acggggtcat tttattagcg accatgatta tcatattgcc ttaaatatcg ccacaatttt
    29581 agcgggtggt gatttaccaa gaaatacatt tatcaatcaa cgttacattc aatcgttgga
    29641 gaaaattggc tttattgact tactaaaatc taaaaaatca tatgaaagaa ttgcacatat
    29701 gttaaaaact ggtaagccat tacgtaatta aaagatagtc attaagagag gatgataacc
    29761 atgcaagaag catacattgt agcttatggg cgttcagccg cagcgaaagc aaagcaaggc
    29821 gcattattcc acgaaagacc tgatgatgtc gcagccaaag tattacaagg cgtattgaaa
    29881 cgtattgacg gaaaattcaa taagaatatg attgaagatg tcattgttgg tacggctttt
    29941 ccagaaggat tacaaggcca aaacattgca cgaacgattg cattgcgtgc gggattatct
    30001 gacacggtac cgggtcaaac agtgaatcgc tactgctcat caggattaca aaccatcgcg
    30061 attgcagcca atcaaattat ggctggtcaa ggagatatac ttgtagctgg tggcgttgaa
    30121 ttgatgagtg ccgtaccaat gggtggcaac gagcccacaa acaatccaac cttacaatat
    30181 gatgatatag gtgcgtcata tcctatgggt ttaactgctg aaaatgtagc atcccaattt
    30241 gacgtatcac gtgaagatca agatgcttat gctgtcagaa gtcatcaacg tgcctatgac
    30301 gcacaacgtg atggtcggtt caaagatgaa attattccaa tacaagtaaa ctcagttgaa
    30361 tatacaaacg caggaccaaa agtacacaca aatatctttg accaagatga atttatacgc
    30421 cctgacacca cgatggaggc attagccaaa ttacgtacag tatttaaagc tgacggcact
    30481 gtgactgcag gaacatctgc cccactttct gatggtgcag gatttgtagt tttaatgtct
    30541 ggagataaag tgaaagaact cggcgtgaca cctattgcac gattcgttgg ttttaaggca
    30601 gtaggcgttg acccgaaaat tatgggtatt gggcctgcat atgcgattcc tgaagtattg
    30661 tcactcagca atctatctgt tgaagacatt gatttgatcg aattgaacga agcatttgct
    30721 tctcaaacga ttgcatctat taaagaagta ggtctagata tatcacgtac gaatgtgaat
    30781 ggtggcgcta ttgctttagg tcatccatta ggtgctacag gcgcaatgtt aaccgcgcgt
    30841 ttacttaatg aaatgggtag acgtcccgat agccgttacg gcatggttac gatgtgtatt
    30901 ggtgtcggca tgggtgcagc tgctatattt gaatatgtgc gttagaatgg ttgattttgg
    30961 atgaagcgga ttcgttttgt tattgaatga agtaggctga agttgaagcc agttgaagtt
    31021 gaagcgggtt gaagcaattt cgttttatta atgaagctgt gtgaaatata gtgattgaac
    31081 aaaaaagtgg tttaatggga tggtggttat ttccgtttta gaatttaaca tttacacgtc
    31141 taattttaat cattgtttta aattttatga atcgaagccc tttgatttaa taatatttgc
    31201 taatgctagt aacttatctg attgttcatg tttaaaataa agaaaaccac tcacatcagt
    31261 gtgtgttcga actagacttg taagttccag ttcggcacga ctttctaaag caattattat
    31321 tgctgtgatt gtcgtatatc acttagatgt gcgtggttta ttttaatagg ttagtaatat
    31381 attaggtcat gttatgttta agactataat gaataaataa tttagaaata tgcttccgat
    31441 tgttcgatgc tttaattcag ttagaagcat catagaatgc atgattactg ttgtaaagat
    31501 acgtaatgtt ttgtattgac tgtatgtctt tggatagagt tacaaactta ttttgttact
    31561 ctaggcccat atgtcgcagt accatctgca tgtgttgtta cattgtatgc atttgtttta
    31621 cttggcttct tgtatgtcgg gcgagctccg tatgacactt gaccgtttgc atgtgttgtt
    31681 acgttgtatg catttgtttt gcttggcttg ttttgtgttg ggcgagcgcc atatgatact
    31741 tggccgtttc catgtgttgt tacgttatat gcgtttgttt tgcttggctt gttttgtgtc
    31801 ggacgagctc cgtatgatac ttgaccattt gcatgtgttg ttacgttata tgcatttgtt
    31861 tctgatggct tattgaatct tggtctcgct tcatatccaa atgttccatc gttgtattca
    31921 cggatacctg taccagcatc tctatattta acatatttag gtgttttgtt aaattgcggt
    31981 ctcggaccat attgagaagc ttctgttgtt tcagttgctt gaggtttaac ttcaatatca
    32041 cttgattctc cttgagtacc ttttaacgtt gattcagtac cttgtggttt tatttcaagt
    32101 ttagatgagc taccttcaag accttctaaa atagggttcg ttaacggtgg gtttgtataa
    32161 ttattgctta atgatgggcc gctttgttcc attgttagaa aatcgggacc ttgaacgatt
    32221 tcaccttgta ccgttttatt ttccatcgtt ggatattccg gaccttttac aatttcacct
    32281 gtaattgtgc cctgtggaat tttaactaat ggttgtgcaa ctggttgtgt tgtttcttca
    32341 gctttaccag ccgtagtttt aacctcttgt tggttatcaa ctttaggtgc ttgaggttct
    32401 tcaactttct tctcttcttt tactactggc gattttgttt cagtttctcc gtattttttg
    32461 acagttttct ttttccaaga atcatctgct tctttaactg cttttttcgt ttcttcaact
    32521 aatttatcaa aattaggttt attatcacta tttgttttat agttatgtgt tgtaggatta
    32581 tatttcgtta tagatttcgg tctattttgt ttagtttcca taaagaaatc atcaataatt
    32641 gaatttaagt catcaatcat ttctttttta atacgttcat ttgtaatttt atgtggattg
    32701 tctgtatctc caaggattaa gtccagtttt gctcgtaact ctttcgcgtg ctccccataa
    32761 tccttatcac cataatatga tacaactaat gtatcaattt cagatacgag atcgtatact
    32821 tccttagttg ctttatcttc ttctgctgca ttaaaagttt tcaagtctga attcttatcc
    32881 ttaatatctt taacttctct gtgaaaatca tccagtgctc tctttaatgc atcctgtagt
    32941 tcattgtatt ctttcatcga aagttcttct aaattatatt tatgaaaatt agccattttt
    33001 aaatctgtac gaggattttc ttttttataa tttgcatacc attgtttata atcttcatat
    33061 tgagatttct ttctctccaa aagatattga tcttccctta ataccttttc caacaaccta
    33121 tctttagctt ctttataaat attatctcca tattcatatt tattagttaa tcctatagca
    33181 taaattatat agtcttccaa ataatataga gctgaattta aatatctgct atctattaat
    33241 gtcccatttt tactcccagc attaacttgt gatttcccac tataatcctt tgttactatc
    33301 gcatctgctt tgttatccca tgtaaataag ctagatgcaa ctgctaatgc gcctagcgaa
    33361 attatttgct ttttcataat tttttaattc ctccaaaatg taattgccca atctacatta
    33421 aagaaacaaa atattaaaag acattaacta tatattaact agaataaaca aagcattaac
    33481 tatctttttg ttatagttaa ttagctttgc aatatacaat agtgtataat ttttttgatt
    33541 aatgtacatc aaaggagtaa caaagcatga caacacaaat gaaaatcaaa acatatttag
    33601 ttgctggtat taaagcggcg ctccttgata cgactggtat taaattagca agcaaatctg
    33661 aaactacatc acatacgtat caacatcaag cgcttgtaga tcaattacat gaattaatag
    33721 caaacactga cttaaataaa ttatcgtacc taaatttaga tgcgtttcaa aaacgcgata
    33781 ttttagctgc gcactatatt gcaaaatccg ctatacgcac taaaaatttg gatcaaatga
    33841 ctaaagcgaa acaaagatta gaaagtattt acaattcaat ttctaaccct ttgcattcac
    33901 aaaacaatta ataattcacc aataaatcat gtaagtgttt gtgacgccaa attgccatac
    33961 aaatacttgt catatgaata taaacgaatg aaacgattgc ctatcccata gatggcaaca
    34021 ttaaaaagac ctctgaaggt atcatcttga taactttaga ggtctttgtt tatattattc
    34081 aaacaaaatt cttatagaac gatcgaagta tgtttcgtct ttcttttctt ctttaatctg
    34141 atcagctaat gctgggtcat ctgtgataat accatcaaca ttggtttgta agtatttcgt
    34201 taaatcttct tcgccgttaa tagtccaagt atagacttct ttattttcca agtgcgcttg
    34261 attaacaagt cttggcgaat aagaaaaatc ttcgatgaca aagaaatcta atgatgtttc
    34321 tttaaaatga ccaaactgca acggaatgat ataaccacac ttgagatatg gcgcttcttt
    34381 tttcaactta gtcatcacat cataatccaa agacatcaca cgatattgat gttcaacacc
    34441 atgctttttc aaaatatcaa taacacgttg tgtataatct gctggttctt taccatgtgg
    34501 ctttaactct actagtagct tcacatttga ttgtttagcc gtttcaataa attcgtctaa
    34561 ggatacaaat tttgcttcat gtccattttg acgcattttc aaaccgacga tatctttgaa
    34621 attagattca gaaatatttt tattaacacc tgttaaacgt ttcaaattgt tatcatgact
    34681 aacaacaaat tgtttatctt tcgtcataat tgtatctaac tcaacgtatt cgacattcgc
    34741 ttttgcagca gctttcaatg acggaataga attttcaacg cctttatctt cgaaaccacg
    34801 atggccaata atggagatat ttgtattgat agtattattg taaagtaagt acatgttata
    34861 accgataaaa catgtcactg caagcaccat tgaaattata aagaacctag acttccgttt
    34921 cggttttgga tatttaaatt ctaagcccgg ttggtctaat acattctctt gttttaagtg
    34981 cagtactaac acactgatta atgataattt cgtaaataaa taatagaaga acaatgcgct
    35041 tttcaataca acaaataaaa ttgatgagac taaaaactta tctccttctt catctacaca
    35101 aatagcaaga tatgttgctc ctgaaataat taatgttaaa atcgcaccaa tgatgagttc
    35161 taatataact atttctataa caagccgaaa cttatttcgc ttcgtaattt gccaacttag
    35221 tctcatattt ttaaataacg actggcggtt taaaatcgtt aacggtagag taaatattaa
    35281 tttaaaattt aatataaata cagcaatcat aaaggtaccg taaatgatta tacctttcgt
    35341 cgttttcata agttcttccg ttaaaaattt aggtatgtaa atattttttg ttaatactga
    35401 acttagtcct aggttggcaa tgggtatcat taacattaaa taaatgacaa agaaaataac
    35461 tggtacacct atgagtttac gcacatttac aaaggcattt ttaaaaatgg atttaaatgt
    35521 aataatctgt cgatcaaagc cggcataaac catataaact aacaatgaaa actctacata
    35581 aatcagaaag gcaacactta atatgaatat aataagaagt atcacactgg cgggatgact
    35641 aacgatttcc gtccaattgt taatcgtaag ttggctttgc ccagctactt ttaacatcat
    35701 attaaatagt aaaattaagt atgtactact aataaaaatc atgattaact gcaatagtaa
    35761 ggcattaatg ctaaaacgcc ctttattttg atacagtaat ttaaatactg cccatatatc
    35821 tttactaatt ctcttcataa tcacgctccg cattgcttta atattaagtt tcatcttaat
    35881 atttttcatt actcagggtc aataaaaatt tgaaaagact catattcata tgcaagtagc
    35941 aaataataac ccattcaaca tcagctaaat gatgatattg gaaccccacc tttaacaaga
    36001 catcacattc tttatcagca tagctactta caaaaacgtc ttccttcaac cattgtagaa
    36061 gttgtctcat atacttacta cgtcttgcta atactcaata ctcaattgaa aaagaagcat
    36121 atgccccttc actcttgaag ttgcatatgc tttcttttcg gtctgaattg tatttataat
    36181 tcaacgggaa ttttcccttt gaagttaaca taacggtagg ctgctttaac agcttcatca
    36241 tcgggcgctt cgacatcttc taattcatat gcaatgccca atgttttcca cttatgaaca
    36301 cctaactgat gatatggcag aatttcaaac ttttcgacgt tatcaagaga attaataaat
    36361 tcccctagtt taattaaatc gtctttatca tcagaataac caggcacaag gacatgtcga
    36421 atccatacag gttgtttcat atctgacagt ttgcgcgcga agttaaggat gtgtgtatta
    36481 ggctttcctg tcaatctaat atgtttgtca ttatcaatat gttttatatc taataatatc
    36541 aagtctgtat gtttttgtaa ttcttcaaaa tgcctttgaa atgcttttgt atcattagca
    36601 catccagccg atgtgtctaa gcaagtgtgc acaccatttt cttttaattc tgcaaataat
    36661 ttttctaaga atggcatttg taacaatggt tcgccaccac tgactgttac accgccaccc
    36721 gatgcatcaa agtatggttt gtatggtaat atttcattca ccatttcatc aactgtgact
    36781 tctcttgatg gctcactaat tttccaagta tctggattgt ggcaatacaa gcatctaagt
    36841 aagcatcctt gtgtaaataa tatatatctt aatcccggtc catcgacagt acctaaactt
    36901 tcgacagaat gtaagtgtcc cttaagcata gtgctcccac cttaaatttt gttacatact
    36961 ttcatggaat gtacgagaaa ttacatctaa ttgttgttca cgtgttaatt taatgaagtt
    37021 aacagcgtaa ccagatacac ggattgttaa ctgtggatat tcttctggat gttccattgc
    37081 atctattaat gtttcacggt taaatacgtt aatatttaag tggtgaccac attgcattgc
    37141 gtaaccatct aacatactag ttaagttacg gttttgatct tctggttctt tacctaatga
    37201 ttttggtacg atactgaatg tatttgaaat accatcttta cagcaatcgt aagggatctt
    37261 agctacagaa cttaatgaag ataatgcacc tttttggtca cggccatgca ttgggtttgc
    37321 acctggagca aatggttcgc cagctttacg tccgtctggt gtgttaccag ttttcttacc
    37381 gtatacaacg tttgaagtaa ttgttaatac actcattgta tgttctgaat cacgatatgt
    37441 tttatgacta cgtaatttag tcatgaagcg ttctactaaa tcaactgcaa tatcatctac
    37501 acggtcgtca ttgttaccgt atttagggaa gtcgccttcg atttcaaagt ctactacaag
    37561 accttcttcg ttacgaattg gtttaacttg tgcatattta attgcagata atgagtcagc
    37621 tgctactgat aaaccagcga tacctgttgc cattgtacgt acaatttctg tatcatgtaa
    37681 tgccatttca atacgttcat agctgtattt atcgtgcatg tagtgaataa catttaatga
    37741 gttaatgtaa acacctgcta gccaatccat catttgatca aatttcttga atacttcgtc
    37801 atattctaat acttcgctgt taataccttc gaagtttgga ccaacttgtg caccagattt
    37861 ttcatcttta ccaccattga tagcgtaaag taatgtttta gctaagttcg cacgtgcacc
    37921 gaagaattgc atttgtttac caattgtcat cgctgataca caacatgcga taccatagtc
    37981 atcgccatag ctttcacgca taatgtcatc attttcatat tggatagaac ttgttttaat
    38041 actcattttt gcacagtatg ttttgaagtt gtcaggtaaa cgtactgacc ataatactgt
    38101 taagtttggt tctggagctg gacctaagtt atctaatgag tgtaagaaac ggaatgagtt
    38161 tttcgtaaca agtggacgtc cgtcaatacc tacaccaccg atagattcag ttacccaagt
    38221 tgggtctcca gagaataatt cattgtaatc aggtgtacga gcaaatttaa caatacgtaa
    38281 tttcatgatg aagtggtcaa taatttcttg aacttcgctt tcagtaataa cgcctgcttt
    38341 aaggtcacgt tcagcataga tatctaagaa tgttgatgta cgacctaaac tcattgctgc
    38401 accgttttgt tctttaattg cagcaaggta tgctaagtat aaccattgaa ctgcttcttt
    38461 gaagttttct gctggacggc ttaaatcgaa accatatttt tgtccaagtt cttttaattc
    38521 ttttaatgca cgatattgtt ctgataattc ttcacgtaaa cgaattacat cttctgacat
    38581 ttctgtagac atcgtgttga agtcgtgcat tttttcttcc attaagaaat ctacaccata
    38641 taaagctaca cgacgatagt caccgataat acgtccacgt ccgtatgcat caggtaaacc
    38701 agtgattaca cctgctttac ggcagttcaa catttctcta gaatatgcat cgaatacacc
    38761 ttggttatgt gttttacgat aatctgtaaa gattttttca gtttcttcgt ctaattcgta
    38821 accgtaagct tcacaagctg ctttcgccat acgaatacca ccgaatggtt gcattgaacg
    38881 tttgaatggc ttttcagttt gtacacctac aattgtttct aaatctttgt ctaaataacc
    38941 agcatcatga gatgtgattg ttgaagctac tttcgtgtcc atatcccaca tgccgccacg
    39001 ttcacgttct tctttcgata actgcattac ttgttcccaa agtttagaag ttgcttctgt
    39061 tggtcctgct aaaaatgaat cattaccttc ataaagagtg tagtttaatt ggataaactc
    39121 tcttacatct acgtgtctgt tccatcttcc atttttaaat ccttgccaag ctgttgcatg
    39181 atttttattt gtttctaaca tactaaacgc ctccatctgt ataatgttgt gaatttgaaa
    39241 tgctatgtta acgttttcat agtcattgta gcatgtttgt tgtgattttt ttcacataaa
    39301 ttatgtacaa ttttcatcaa atgtatgtca aaacatagaa actgtactaa tttgtgtaac
    39361 agtaatatgc taattgcatt ctgttacaca acagttataa tgttcactat tttcattgct
    39421 ttaaaagtta atttaggagt atatatttgt ttataaaaaa tgattgttga taagcgttaa
    39481 atcaacactt agaaagattt acttttatat atgaagagac tggattaaat acttttattg
    39541 acgtaaaaat tcacttttga accgttcaat atcttgccga tttttatata acagctacaa
    39601 ataaaatata acagtttgat tttacagcct cggtaaatcg tcttgacaaa caaaaatttt
    39661 gtgctatcac aacatttgca acgtcttaac aagtcatcta taaacatttc taaatattta
    39721 acattactta tgcgtcattt attgctaaaa ttattgtatt aaaatataca tagaattgat
    39781 gggatatcat gaaatcaaaa atttatatct tgctattatt tctcattttt ttatcagcat
    39841 gcgctaatac gcgtcactct gaatccgata aaaatgtatt aacagtttat tctccgtatc
    39901 aatcaaactt gattcgtcca attttaaatg aatttgaaaa acaagagcat gtcaaaattg
    39961 aaattaaaca cggatctact caagtactgc tttcaaactt gcataacgaa gatttttcgg
    40021 agcgtggtga tgtctttatg ggtggtgtgt tgtcagaaac aattgatcat ccagaagatt
    40081 ttgttcccta tcaagataca tctgtaacac agcaattaga ggattatcgc tcgaacaata
    40141 aatatgttac tagttttcta ttaatgccaa cagttatagt agtgaattca gatttacaag
    40201 gagatattaa gattcgaggt tatcaagatt tattacaacc tatacttaaa ggtaaaattg
    40261 cgtactcaaa tccaaataca acaacgacag gctatcaaca tatgcgtgct atttatagca
    40321 tgcatcatcg agtaagtgat gtgcatcaat tccaaaacca tgcgatgcaa ctgtcaaaga
    40381 cgtctaaagt cattgaagat gttgcaaaag gtaaatatta cgcaggtcta agctacgaac
    40441 aagatgcacg cacatggaaa aacaaaggtt atcctgtatc aatcgtttat ccaattgaag
    40501 gaacaatgtt aaatgttgat ggtattgctt tagttaaaaa tgcacaccct catcctaagc
    40561 gtaaaaagtt agtgcaatat ttaacaagcc gctcagtaca acaacgatta gttgcagagt
    40621 tcgatgccaa gtcaattcga aaggatgtta gtgaacaaag cgatcagtct atcgaaaatc
    40681 ttaagaacat acctttaata ccgaaatcta aattaccgga tattccacat cataaatttt
    40741 tggagatgat tcaatgacgg catacaaacc ttatagacat caattaagac gatcgttatt
    40801 tgcgtctact atttttccag tatttttagt cattattatt ggactagtaa gcttctatgc
    40861 catctatatt tggattgagc atcgtacgat tcatcaacat gttgatgaaa gtcagtcatc
    40921 tttacatcat actgaaaagc agattcagac atttattaca cagcataata attcgtttca
    40981 agagctcgat ttaacaaatc atcatgatgt gaccgctaca aaacgtgaat tattaaaatt
    41041 aatacaccaa cagcccgcaa cactttatta tgaattatct ggaccaaatc agtttataac
    41101 caataattat gaacatttaa acactaaaaa tatgtattta ttttcaacgc atcagcttaa
    41161 attcaaaaat agtacatata tgcttaaaat ttatatggct aacaccccac gtttaagtga
    41221 aattaagaaa gataaccgac aattcgcact tatcgttgat caatatgaca atattttata
    41281 cgccaacgat gaccgtttta ctataggtga aaagtataga ccacaacaat tcggatttat
    41341 gaatgaatct gtaaaattaa accatgctga ccatcgtctc attatttata aagatataca
    41401 tgaaaacatt gaagatggta tcacgttgtt aattgttatg gccgtagttc ttgttttact
    41461 agtaatattt ggtttcatta gcgcagataa tatggctaaa cgacaaacaa aagatatcga
    41521 aacgattatt caaaaaattt actatgccaa aaatcgtcat ctaggtacat atacgccttt
    41581 aaaaaacaac agtgaactag aagaaattaa taattatatt tacgatttat ttgaatcaaa
    41641 tgagcaactg atccattcta tcgagcatac agaacgtcgc ttgcgtgata ttcagttaaa
    41701 ggaaatagaa cgccaattcc agcctcattt cctattcaat acgatgcaaa caatacaata
    41761 tttaattacg ttatctccga aattagcaca aactgtggtc caacaattat cgcaaatgtt
    41821 acgttactca ttacgtacaa actcacatac agtggaatta aacgaagagt tgaactacat
    41881 tgaacagtat gttgcaatac aaaacatccg ttttgatgac atgattaagc tccatattga
    41941 aagttctgaa gaagcgcgtc accagacaat tggtaaaatg atgcttcagc cactcataga
    42001 aaatgctatt aaacatggta gagatactga gtcattggac attactatac gcctgacatt
    42061 ggcacgtcag aatttacatg ttttagtttg cgacaatggt atcgggatgt cgtcatctcg
    42121 cctgcaatat gtacgccaat ctcttaataa tgatgtattt gatactaagc atttgggatt
    42181 aaatcattta cacaataaag caatgattca atatggttca catgcacgac tccatatttt
    42241 ttcaaagcgt aatcaaggta cgttaatttg ttataaaata ccactttcaa gggggaatgt
    42301 agatgtttaa ggtagttatt tgtgatgatg aacgaattat tcgagaagga ttaaagcaaa
    42361 tcattccttg gggagactat catttcaata caatatacac tgctaaagat ggtgttgaag
    42421 cattatcact aattcaacaa catcagccag aattagtaat tactgatatt cgtatgccac
    42481 gtaaaaatgg cgtcgattta ctcaatgata ttgcgcatct agattgtaat gtcatcatat
    42541 tatcgagtta cgatgacttt gaatatatga aagccggcat tcaacatcat gttcttgatt
    42601 atttattaaa accagtagac catgcacaat tagaagttat tttaggaaga cttgtccgta
    42661 ccttattaga gcaacaatca cagaacggcc gtagcttagc atcttgtcat gatgccttcc
    42721 aaccactatt aaaagtagaa tacgatgatt attatgtaaa ccagattgtg gatcaaatta
    42781 agcaatctta tcaaacgaaa gtgactgttt cagatttaat tcaacatatc gatgttagtg
    42841 aatcgtatgc gatgcgaaca tttaaagatc atgttggtat tacaattgtc gattatttaa
    42901 atcgctatcg tattttacaa tctctgcaat tgttagatcg acattacaaa cactatgaaa
    42961 ttgcagacaa agtaggcttt tcagagtata aaatgtttag ctatcatttt aaaaaatatt
    43021 tacaaatgtc gccaagtgat tattgtaagc aagcaaaata gattagcaca taactaatga
    43081 ttatggcgcc tccattttgt tctatgaaaa agcggactga tagagaagtt ttcacttttc
    43141 tttcagtccg ctttttagag agtttaaata taaaacaata ctccctacaa tattgacacc
    43201 gctatatgtc tttattgtaa ggagtaaatt cattgagtca ccctaagtcg ggcaaataag
    43261 gatacttcat aacagggaat tatgatctgc cccgagacat agagatgata tatttgcaag
    43321 ggtagaagca gtatctttca tctcttcacg cacatgccgt cggggcagga ttgccaacta
    43381 tattgcaata gattggcgat acttttaatc cgatttatat taaattttta aacttctaat
    43441 tttcttttct tcatagaaag caacgattcc taatagaatc atgcctagga ataatgcaac
    43501 atagaagacg atgaaaacat ctgtccatcc acttaatgta tatccaaaga tgtttaaacc
    43561 gttacgtgtt ggatcagcaa tagccgccaa accaactttc gccattgagt caccgaatag
    43621 atacgcgaat gaacctgtca ttccgtttgc tacactgatg gcatttttag gaacaaaacc
    43681 agtcaatgat acaccaatta ataattgcgg accaaagatt aacgcaccta atgcaaacaa
    43741 tgaaatgtta accatcatga cacttgtagc atttgtgtag aataagacaa caaatgtaat
    43801 cataaacata cagccaatag ctacaattgc acgacgacct tttaataagt ctgatacgta
    43861 gccccataat aaacttgcaa ctaatgcacc aatttcaaag tagaatatcg tattaactgc
    43921 atcgccttta ctaaagtgta aatgctctga cacatataac ggtgcccagt tatcaatacc
    43981 gattcgtaca atgtatacaa agacgtttga aacacataga atccatataa caggatttcc
    44041 caggatatat tttttaaaga tctcccattt cgtcatacct tgagaatcaa tattttcttt
    44101 atcgaccggc tcttcccaaa tttcttcagc acgattccat cctaattctt ccggatcatc
    44161 ttttccgata aataatgttg cgataccaat aagtaatgca atcaccgatg ggaaaatgaa
    44221 catccctata acatttccat ggaagaatac attagcaccc caaagtgcaa caccacctgc
    44281 tatggcacca ccgatattat gtgatgtatt ccagaatcct aagtatcggc cacgtttcgt
    44341 tcttggcgcc catcttgaaa tcgttgaata acttgcaggt ccaccaactg attggaacac
    44401 cccgttaagt ccccaaagta caattaataa tcccattaca gaaccaaagt aacttaaaac
    44461 aaatcccata attaaaactg taatcgcaga taagataagt aagaacgaga taatacgttt
    44521 tgtgttacgt ccatcgacaa aatatccaag taatgttttt cctaaaccgt acgtgatact
    44581 aaatgctaat ccgatataac caagttctaa tgtagataat ccaatttcct cttttaaaaa
    44641 cggttgtgcc gccttaaagt tgtttcgaat taaatacata gccatataaa caaagaacac
    44701 tacgaagaaa gcttgcatga agtttctaag ccataattta cgttgtaccg ataatggaat
    44761 gcctttgttc ggaatcttat ggatatcaaa aaagttcatc tctgtcacct caatcatttt
    44821 cgttaagcca aatatatcac gtcggtgtaa gcgcttaata tccttaaaat tattaaatta
    44881 tccaaaatac tgaacataaa cacatactta gaaacatatt tatgtactaa aattaataaa
    44941 tagttaatag tatttgacaa gctttatcta taaaatactt tatgttgtca aaatcaacgc
    45001 ttatacatat gacataatga cggaggacat catgattcta tctatattac ttatcttttt
    45061 ctgtatcaga cttgtcagct taaagatatc tattaatcac tcaaaacaat taaaagcaga
    45121 cggtgcagtt gaatatggcg ttaaaaattc gaagttttta gcaataacac acgttttaat
    45181 ttatgtattg gctggtgtag aggcatttat taataaagat acatttagct ttgcaaatgg
    45241 tattggctta gttatattaa tctttgctta tatcatgtta tttatggtta ttaaaacttt
    45301 aggtggtatt tggacattaa aattattcat tttacctaac catcctatta ttaaatctgg
    45361 attatataaa attacaaaac acccaaatta cttcttaaac atcattcctg aattaatcgg
    45421 tgtattatta ttaacacacg caacatatac aactatttta ttagtaccgt atgcgtattt
    45481 cttatatgta cgtattaaac aagaagagaa attaatgaat atttaaaaag atgaaagcct
    45541 gtcatcactt tagtctacat cgacgtccat ctttaaagtg cattctgaaa tattatattc
    45601 agaaaccttt aaagtatgtc gtgtctatga ccattgtgac aacaggcttt tttttattat
    45661 catatacctc actattgaaa aggttatata ctcttttcaa tggtgaggat tgttactgtg
    45721 aatgggtatg accatcgcta tatacttata taaagtacac cttctttaca aacaatgact
    45781 tgatttaagg tgtgaaattt ttggcaatgg cttggcgaca tatgcattta ttcaacactg
    45841 ccaccagtct tacttcagac aaacttcaga ctaacctcga gttcgtatta tgcccaccac
    45901 atgtctgctg gcttgtcgta aatattaaca gatttcaaag tttgacaagc tttttggaaa
    45961 ccttcttcta ctgacataat aggatcttca tgttcaatac ttaatacata atcatatcca
    46021 ttaataataa gttgacttat gatatctgcc catacatatg gactatgtcc ataaccaact
    46081 gtacggaatg tccatgctct tgtcgcaacg ttaccatatg gttgcatatc agttagacca
    46141 tacatattta cattttcttg attaatatac gtatctttag catggaagtg atgaattgca
    46201 tttgcttggc ctaatatgcg aatcgcagca attgggtcaa taccttgcca ccatagatga
    46261 ctaggatcta agttagcacc gatatattca tttgtagcct cacgtaactt caacattgta
    46321 tatggtgtat gcactaaaaa tcctgcatgc aactctatgg caatttttac atcttgctct
    46381 tttgcaaact cagctaaatc ttgccaatat ggtatcaact tttcattcca ctgataatca
    46441 taaatttcag agtaggctgt tggccaaggt gtaacaggcc aattaggctt tttagcggta
    46501 tcatctgatc ctgcaatgcc agaaaatgta ttaacaactg gcacgtctaa tagatttgct
    46561 aaacggattg ttttacgtaa cgtttcatcg gcttctttcg cttctatcgg atctggagaa
    46621 attggattgt tatgacaact gaaaccactt atttgtaagc ctctgtctgt gattgacttc
    46681 ataaatgctt ggcgcttgtc ttcattttct aacaactcat ctaacttaca aaatttatct
    46741 cctgggttac cacctgttcc aacttcaatc atatccaatc cagattctga gacataatct
    46801 aacatatctt caaaattttt atcgtaaaat aatactgaaa atacacctat tttcattgaa
    46861 aagcactcct tctttttata ttgagaattt tgtatcatat atgataatca ttaaagttgt
    46921 atgctcttgt tatcaagatc gctacgataa atcgcttcta taagggcatt tacattgcgt
    46981 gcttcttccg gtttcaccac aatctcttct atccctaaac acgcattgac aaagttacgc
    47041 gcctgtctct caccagcaat gtcttcgtta tgctcaacat tagctttgct ttcaaaaata
    47101 gttccaaagc ggggctcata tatttcaaat ggaaataaat tgataccgcc atcttctcct
    47161 gataaactaa cgtgaacctt atcttctttg atatttgcag accacgaaca ttcaaactgc
    47221 atgcttgctc gattggcaaa tgtcatataa ctagtaacat gatcatcgac atcaaattta
    47281 gtatgatcaa atgttcccca atcattaatt tgattcggtt gtttgctcaa ttgattatat
    47341 gtttttccta gcacttcatg cggcaccata tctttaccta gtagccacaa agataagtct
    47401 aacaagtggc aaccataatc gattaaacta cctccacctt gcaacgcttt attggtaaaa
    47461 acaccccagc caggcacttt acgcctacgc atcgcttgta cacgtgctac taaaggttta
    47521 ccaaccacac ctgattcaat tgctttttta gcagtaattg ccacatctgt gtgacgataa
    47581 tgatatgcga cagttaataa tttgtgattt ttattagccg cttcaatcat gcgatcacac
    47641 tcttccgtcg tcatcgccat tggcttttca cacaatacat ggacaccatg gttcaatgct
    47701 tctatagaaa gatcagcatg gaatttatta ggtgtacaaa tgaccaccgc atcaacaagt
    47761 ttaaacagct cgctaggtgt ctcaactgca tgaggtatat taaagcgctt cgcaacatca
    47821 atcatctgca ctgtattaat atcttgtact gcaactaatg agactgtgtc tttgagtttc
    47881 agcaatgctg gaatatgacg gtcttgtgca ataccaccaa cacctatcac accaactttt
    47941 aattttgtca tgatgtgcct ccttaccgta tgatgttatt caaagtaaat tgctttgcct
    48001 gattttgcag actgataaat cgcttcaaga atttttgtaa ctaccattgc ttgttccggt
    48061 ttcacaactg gttcagtatc atttacaact gcatcaatcc aagcttttgc ttcttcttca
    48121 gcttcatcca cttcattacc ttcataaaag tcgacgcctt tgttttccaa ttcaacgtgt
    48181 ttggtataaa gtgtacccat gtcttcacca tgaatacgta gaccatcttt catatcagca
    48241 cctgctttag ttcctgataa tgaacatttt gcctcatcca cttctaaaga attaatcgcc
    48301 caagcggatt ctaaaatgat cgtcgctcca ttcttcattt taataaatcc aaacgcagaa
    48361 tcttcaactg taaattcatc tggattccat gaaccccaag cgtttgccgc atgatgctgt
    48421 ttatttaatt tatggaatgt tgaacccatc actgattctg gttcataatt atccatcatc
    48481 cataacgtta aatctaaagc gtgtgtaccg atatcgatta atggtcctcc accttgagct
    48541 tcttcgtcta gaaagacacc ccatgttggt actgctcgac gacgaatggc atgtgccttt
    48601 ccgaagtaaa tgtctcctaa gtcgccacgt tgcgctgctt gatgtaaaaa ttgactatct
    48661 gctcggaaac gattttgata acctattgtt aattttttac ctgttgattt agctgtatct
    48721 atcatttttt gagcttctgc tgtcgtttta gccattggtt tttcacacat cacatgttta
    48781 ccagcatgca accctgctac agtaatttca caatgcgagt catttggcgt acaaacatgg
    48841 ataacatcaa tcgtgtcatc ttttaacaat gctttgtaat catcataaac ctttgcattg
    48901 tcagttccgt atgcttctgc cgcactcgct gctttcgaaa tgtctacgtc acaaaatgcg
    48961 atcatttcaa cattttcaac tttttgtaaa cttggcatgt gcttgccatt cgcaatacca
    49021 ccacacccaa tgattccaac tttaatcgtc atcataacgt cctcctttta atctttatcg
    49081 tcattagtgg ttctttatca attctgcccc actcctaatt tcaaactaac cttttgtcgc
    49141 acctgttgtt aaacctgata ctaaatagcg ttgcaagaac aagaatacga ttgctatagg
    49201 tactgcaatc ataattgccc ctgctgcaaa cactgtgaaa ttatttgcat acttatcatt
    49261 aataaagttg aacaatccaa ctgctaatgt gaatttttca ggacttctta atagtatttt
    49321 aggtaatata aagtccataa atggccccat aaaattgaac aaagcaacaa ctgctaaaat
    49381 cggcttagct aatggaagca taatttgtaa gaaaatacgc atatgccctg caccatcaat
    49441 tttggcagat tcatcaagtt cttttggaat cgtatcgaag taacctttca ctaaaaaggc
    49501 attcatcggt attgatccac caatatatac cagtgttagt ccaaataaag aatctaataa
    49561 tccaattgta tttagcaaaa tatagattgc gaccattgcc attaatacag ggaacatttg
    49621 taaaatcaaa aatgtaatca gcccgtattt acgaccaaca aagcgatatc tagaaaaagc
    49681 atatgctgtt aacgtgacaa atatcacact aaacagtgca tttgcagatg ctacgataag
    49741 cgtattttta taccaagtca ggtattgact actgtcatcg aatagtaaga atgcataatt
    49801 tttaaatgtt gcattgtctg gtatcatttt ggcaccatac aagttcgtac ctggattaag
    49861 ggaaatgcca aatgtccaca gtagtggata taaaatgatg acaaacatca tcgctataaa
    49921 actgtaaata ccgattgctt ttaatatgtt tttcttcttt gtcatcttat aaacctccct
    49981 catctttaaa cgtacttgta cgtctgaatt gaataaatgc gacaatagca acaataaatc
    50041 caataattaa tgacacaact gcacccatgt tgaagttgtt aaactcaaat gtcagattat
    50101 acacccaaga tatcaagata tctgtactac cagcattctg ccctgacact ggtggaccgc
    50161 ctttattaaa tagataaata agattaaaat tattgaaatt acctgcatat tgcataatta
    50221 acaatggcgc tgtggcgtaa atgacatgcg ggaatgtgat gtttctaaac ttttgccaac
    50281 tagacgcacc atccatatct gctgcttcgt accaatctga tgaaatactt tgcagtactc
    50341 cagtgaacag tgcaaagaca aatgggaatc caagccatac ttgaatgccg attaatgcca
    50401 cttttgccca aaacggatca cttaaccatg ctggtgctac acctaataaa ggttgcaaaa
    50461 tatcattatt tatcgcacca aattcatcat taaataacgc tacaaatatt aaaattgtca
    50521 caaatgatgg tacagcccaa ggtaggatta acacagtacg gataaatttc ttacctttga
    50581 cgacagggtg atttacaata attgccaaaa acagccctaa tgcaatttga agtgtcgttg
    50641 caacaagcgt ccatactaat gtccaagtaa taacactgaa aaatgtttta cgccaaacgc
    50701 caattgtgaa taacgtttta aagttatcta aaccaaccca ttctaatgtg tgtctcggag
    50761 gcgcgttgta taaattgtaa tttgtaaatg ctactccaaa cataaatatt aatggaaata
    50821 caactacaaa tatcaataag aatgtacctg gtgagattag taagtatggg aacgtcttgt
    50881 cccatgttgc caccatacgc gccttcggat cctttatttc ctcattgcga ttaaatcgtt
    50941 cagcattacg atatgcatct aaaatattaa tgatatatag catgattgcg aaagcaacga
    51001 gtaagataga aataatacct tgtgcaagta agacacgaga atcgtctaac ttaggtactg
    51061 tccctaatgt gaacaatccc caaaaaccaa tatttaaaaa gctataaaaa acagaaataa
    51121 aactgatgaa aaagataaaa aatatcgtcc ctttaatggg tcttttatta taaaactgtc
    51181 ccaaaccagg tataacagat aataatgccg ctaatttagg gttacgtttc gtcataacta
    51241 atctcctttc ttatcatttt gtgatggatg aagaatctta atattttgcg ttatatcatt
    51301 cgtcgcctca tctaacgctt gtttaggatt cttaccattt gaaataaata tgcttgcatt
    51361 gcccatcggt tcccaaactt gtcgcatttc aggaatatta ggcatcggtt cagcatgacg
    51421 tgcttgcttt tcaaacactt ttaaatttgg attagatgat ttcacgtcaa cacgtccagt
    51481 aatttcgctc atttcatctg tatatttttg taatgtatct ttactagtga tatacagcat
    51541 taaatcttta gcccaatact tatgtttact atattcagat aaataccaac cacgtacacc
    51601 tagaaatggt ttcataggtt tgccaccatc tgtaggtaat gttgttactc ctaaatcttt
    51661 accaaacgtt tcttgatatt cattaatgtt ccacggtcca gtgacaaatt gtcctacttt
    51721 tccttcttta aaaagaccaa tcatgacatc atgtgttgct gccttaggaa gatacccttt
    51781 gtcgtaccat ttttgtaatc gttcagcatt cttgacgaca tgttttgaat ttagtcctag
    51841 ctgatgaata tcatattcac tgccattttt cttgaaaata taatcatcat tgccgaataa
    51901 aaacggataa ttaaaataga aatttttagc atcaaataac ataccgtatt gtttcttttt
    51961 actatcagtt agtttggcag cattagcttc aacttcttct aacgtttgcg gtgcattttt
    52021 cactaatttt ttattataaa aaagtgcggt tgtttcaacg atagctggca atgctagttg
    52081 cttattgtca taattcatcg ctttaagtgc ttgcttattg aaacctttca actcatcttt
    52141 tgataatttg atttcagcag ctaagccttg tagataggca cttccagtat tatcatgtgc
    52201 taagaaaaag atatctggac cttttcctgc aggagcgtct agcgaaatat tttctagttg
    52261 atcattttga ccaatattta caagctttac tttgatgcca gtttttttag tatattgatc
    52321 cgtaattttt ttataaaacg ccatttgctt gtcgccatcc acccacatcg taagttggtt
    52381 aggcttgtct ttagaattat ctttattcaa tgctttatca atatcttctt tcgaacgatt
    52441 agggccacat gcagttacga ttaataacat taccacggct aacgtgatac attttaaaat
    52501 tttagacatg aatactcccc cttagacgat acgatttcct gttttttcat caaaaaagtg
    52561 acacttattc atatcaaatg ctagtgtaat cttgtcgttc gccatcactt gagttcttga
    52621 atctaattta gaaatcaatt ccattccttg gaatgtgcta tgtaccataa tttctgaacc
    52681 taacagttcg gatacaacta cttcagattc aaattgtgtc tctggagaag tttgaataaa
    52741 tattggttct tcatgaatgt cttcagctcg aatacctaaa ataatttcct tgtctaagta
    52801 gccagcagct tttaactttt caaatttttt attatgtaat ttaaaatggt gtgttcctac
    52861 cttcaatccg tccatttcaa ctgtagcatt caacatattc attgctggtg agccgataaa
    52921 ttgcgccaca aatatgcaat taggggcatc atatatttct ctaggtgtgc cgacttgcat
    52981 aatgtcgcca tctttcaaaa caacaattcg actagccatc gtcaatgctt cagtttgatc
    53041 atgtgtaaca taaattgtcg tagtattaag tcgcttatgt aatttcaata tttctgtgcg
    53101 catttgtact cgaagcttcg catcaagatt cgataatggt tcatccatta aaaagacttt
    53161 cgcatcccta acaatagctc tgcccaaagc aacacgttga cgctgtccgc cagataacgc
    53221 ttttggttta cgaccaagat actcagttaa tcctaatatt tcagctgctt cattaacttt
    53281 ttgttcaatc tcttttttat ttactttacg tagctttagc ccaaatgcca tattttcaaa
    53341 aacagtcata tgtggatata atgcatagtt ttgaaatacc atcgcaatat ctctattctt
    53401 tggttcaaca tcgttcatgc gttccccatc aatataaaaa tctccagatg tgatagactc
    53461 tagtccagca accattcgta atgttgttga tttaccacat cccgatggtc caacaaatac
    53521 aatgaattct ttgtcagtaa tatgtagatt aaaatctttc actacagtat tgttgttatc
    53581 atacgtcttt ttaatatgct ctaactttag ttctgccatg ttatgcctcc catactttgt
    53641 ttacagtttg attgtgcaat acacttcgtg aaaacgtttg cacaaatact tacataaatc
    53701 atatattcag tcattattaa tgtcaatcat aaattataaa cgtttacatt actgtcagaa
    53761 ttaaactttt atacagaaaa ttgatatagt gcacaactat tttctagtgt gacaataatg
    53821 ttttttatgt gcgatatttc actgccaatg ctaacaacct tgtcttacat tttaagtaat
    53881 atctaaatca aacaagtatg acttagcatt acgattttca ttgaaaaaag caaccattat
    53941 gtaagtatca tactcacaca atgattgctt aattttttag actgcgggca cccttctata
    54001 actacaaatc atataggcta cgttccccat caggtaactg atcgataaac ggttttggat
    54061 cttctgcata tgcattacct acgccaccct tcattctttg aaaatgtaaa tgtgcgcctg
    54121 tcgtttgtat acctgtattg cctgaatatg caataatatc acctgctttg actcgatcac
    54181 ctactttgac attatatttg tctaagtgta gataccactg gtgatattct ccattgtctt
    54241 cggcaatctg taataccttg ccgcccaatt cattattaaa gatacgtgtt actttaccat
    54301 tcgtcggcgc tttaattggt gtaccttttg gcaatgcaaa atcaatgcca taatgcttac
    54361 catcaaatgg tgaatgttga tatttaccaa aagtctcagt aatccgactc cctctaaaaa
    54421 agtcttccca ttttgagtgt ccttgtcctt tatgattttg tgtcaattta tcggtatatt
    54481 ggttaaacca tttttgaatg tcatctatat aaataatcgt aaataatagt gaaagaaaaa
    54541 tgataattgt tgccaaaata cgttttggtc gctttgtcat agtggcatcc atccttcata
    54601 tattttgggt attaagtata tattctaaat caactatttc ataattaaat gcactcatac
    54661 tttagtagta ctgccaaata tagatgaaga aaatgatggt aatttgaata ttttgattga
    54721 tattaaattt tatatcgagt ataataatta tacttaagaa aataacagaa acagaggaga
    54781 attttcaaat ggcaaaagta ttatatatta cagcacaccc ttttaatgaa ctggtatcaa
    54841 attctatggc ggctggtaaa gcatttatcg aaacatatca acaacaacat cctgatgatg
    54901 aagtgaaaca tattgattta tttgaaactt atattccagt tattgacaaa gatgtattaa
    54961 ctggttgggg aaaaatgagt aatggcgaaa cgttaactga tgatgaacaa atgaaagttt
    55021 caagattgag tgacatttta gaagaatttt taagtgcaga taaatatgta tttgtaacgc
    55081 ctatgtggaa cctttctttc ccacctgtag ttaaagcata tattgatgct atttcaattg
    55141 caggtaaaac ttttaaatat tctgctgaag gaccacaagg tttactaact gacaaaaaag
    55201 tattacacat tcaatcacgt ggtggatatt atactgaagg acctgctgct gattttgaaa
    55261 tgggtgaccg ttatttaaga acaattatga cattcttagg tgtgccatca tatgaaacaa
    55321 tcattattga aggacacaat gctgaacctc ataagactga agaaattaaa gcaactagta
    55381 ttaataatgc tgaaaaatta gcaacaactt tttaaatttt tagttacact gtgtgacgtc
    55441 tcaaacgtca tgcagttttt tattgtatct cttttccgct ttttataaat atctattatt
    55501 ttcataaatt taaaatttga tattcacttt aatactgatg taaatatttt tcaaaatata
    55561 ttattatagt gttaatgaag tattttgaga aacggtggga atgtatgaaa gcacataaaa
    55621 ttttctggtt aaacttagca gcaattatta ttatttcaat tgtagtcagt ggcgatatgt
    55681 ttttggcaat gaagtgggaa caaattcatt tgaaagatgg tcttaaaaaa gtactaagta
    55741 cgtatcctat taaaaactta gaaacgcttt atgaaattga tggtcacgat aatccccatt
    55801 atgaaaataa tgatcaagac acatggtata tagaatcctc ttattcagtt gttggatcag
    55861 atgagctttt aaaagaagac cgtatgttgt taaaggtaga taagaacaca cataaaataa
    55921 ctggtgaata tgacacaact acaaacgata aaaaaaatgc tactgactcg acatacaaaa
    55981 gttatccagt aaaagtagtt aataataaaa tcgttttcac aaaagacgta aaagatcccg
    56041 cgctgaaaca aaaaatcgaa aacaatcaat ttttgattca aagtggtgat ttaacaagca
    56101 ttttaaatag caatgatttg aaagtcacac atgatcctac cactgattat tataatttat
    56161 ctggtaagtt gtcgaacgat aatccaaacg ttaaacaatt aaaacgtaga tataatattc
    56221 ctaaaaacgc atcaacaaag gtggaattaa agggaatgag tgatttaaaa ggcaataatc
    56281 atcaagatca gaaactttat ttttattttt caagtcctgg aaaagaccaa atcatttata
    56341 aagaaagcct tacttataat aaaataagtg aacattaata cttatgctgt aattatagaa
    56401 acatccaaat catctattaa aatcctatat tataaaaaca cctcacataa ctcgttcaac
    56461 tgtaccaaac cacattacat tagattttag gctaactatt gtgatgtaca tcaaaaacga
    56521 atttgtgagg cgttgtatat tttacaaagg tgactagcgt ttcgtatagc atttccaaca
    56581 ttactacact caagcgtcac gctaaagttc gaaatcgaat cctttcattc aacaaaagct
    56641 catatccact acaaacttca tatcaagcgt ataaactatc ttgtgatact atctcgatca
    56701 tatctatagt atgcatttgt gttccgtttc actgaagtat atgtatcatc agttaagtat
    56761 aaaccgtcat ccttcaatgt tacttgataa gcatatttcc gtgctaacca ggcaatatct
    56821 atataatttt ctcctgcgtt ttcataactt cttaaatctt caatatgtgc actaacttca
    56881 gggaaaatga ttctaacaac actttcatca acccaatatt tgtcatgcat ccatcgcact
    56941 tgatctgcca ataaaggtaa ctgcacatca ttgaaatata gacgaaagcc gtcactatca
    57001 tacatttgcc gatatggtaa tggctgtttt ctaatcacta acacctcgcc acccattacg
    57061 gtgccttctc tagtatcatc acttccaccc gaagcttcat acgttgttgg gtcaacctgt
    57121 agtccatgta catctccaat ataagcatct ggtttatgtt ccattgcatg tccatgtgca
    57181 atcaatgcta atattgtaga ttgtgaaaat tgaggctccc attcaatgcg attaggatgg
    57241 ctactataaa ttctaggttc atctatagcc tgctgaatat ccatgccaaa cactaataca
    57301 ttgattaatg tttgcgcaac actagcaatg atacttatgg caccaggtgc acctactgtt
    57361 aatattggct tcccgtgata catcacaatc gttggagcca tgttacttag tggtcgttta
    57421 tatggtgcaa tttcgttaat accaccatct actacatcaa agccatccat tgtcgtattc
    57481 aataacacac cgtagcctgg aatcgtgata cctgaaccat aaatcatacc aattgatgtc
    57541 gtaaatgaag caatattacc ttccttatca atcacacaaa aatgagttgt ttcggtatga
    57601 ttttcttcaa catctgtatg actaatgcaa tcagacacaa caccatgctc tatatcaata
    57661 tttgctttat tgctatcaat gagcgtactg cgtgctttta aataatcatc atcaattaat
    57721 gactgtacag gcacctcatg aaaattatca tccgccaagt attgcgcacg atcactatat
    57781 gctaaatgca tcgcttgtat caaatgatgc aagtaatcaa cagatcttgg acccatagat
    57841 ggtaaatcga catgttctaa taacttcaat atttgaatta ccgtgatacc gccagaacta
    57901 gatggtccca ttgaataaat gtcatagtct ttaaatgttg cactgattgg cgctttaatc
    57961 tgaatgtcat atttggctag atcctctaaa gtgattgtcc caccacatgc tttgacaaca
    58021 ttgactaatt gtttcgcaat gtcaccttta taaaatgcat taaacccttg ttctcttaat
    58081 atttgaaatg tcttacctaa ttcgggttgt acaatccaat caccttcacg ccaatattga
    58141 ttttcatgcg taaatacttg tgccgtttca tgatactttg tcaatcgtgc gtgttgctgg
    58201 cgcgaatatt tttcagtagc ccaattggct gcatgacctt caatggctag ttcaattgca
    58261 ggattaatta aatcttccaa tgacaattta gcataacgct tgtgaatata atcaaacagc
    58321 tttggaattg ctggcacagc gacagtttta ccatgtgtag tcatatcaaa aaatgattta
    58381 tattcgcctg aatcatctag ataaaattgt ttgtctacat gttcaggtgc tgtctcacgt
    58441 gcatcaaacg cagttatact gccagtactt tgctcataat atagcaaata cccgccacca
    58501 ccaatacctg atgcaaatgg ttctaacaca ttcaatgcca gttgaattgc aatcactgca
    58561 tccatggcgt tgccaccttg atctaataca tccttaccaa ttttagccgc aagaggatgt
    58621 gatacggaaa ttaacccttc tttagatgtt tttgtctgtt tgtcatttaa gttaatgacc
    58681 atactatatc ctcctacttt ctgttaaata tttaaaacat tattgattaa tggctttttc
    58741 tactttttct aaatcttgac gttgctcgtt accagtatcg acaagtggtg taatcggtga
    58801 tgcaatttta aatttatcgc cacgataaaa cttaataaat tgatcctgat ctatcgcatt
    58861 aactactgct tgtctcaagt ttggatgcgt cttaaatata ccttttttaa tatttagcat
    58921 taaaaagact gacttgcgtc catttttgcg aataatgctt aaatttttat ccgacttaat
    58981 taaatcaaaa tgtttttgat tcacatctgc caacatatca attgaatgat ttctaagttc
    59041 tgacaatgca ttattcgggt caccattaaa cttcaatgta atatttttaa ttttagctgg
    59101 tccataacta cctttttctg tttcgttgaa tcctggatta cgttgaaacg ttgcttgata
    59161 tgcatttttc tgtgtcataa tgtatgcgcc acttgcatac agcgcatttt tcccatctga
    59221 atttgcagga attgtactgc tatccccata tccttttgga tattcttgat ttacttgatt
    59281 aacaaatttt ttagataaaa tgcctgccga agagtgtgtt aagtaattta cctctcgagg
    59341 catcgattga tctgtcgtaa ttttaacaat ttgataaata ccgtctttat tatttacttt
    59401 ttgaccatct gtcgttaacg atttgacgtt ataagcttta atcaacttat catagattga
    59461 tttatcgtcc ttgtctttct ctttacgcaa ctgatcgatg tcctcatctt ttaatatctt
    59521 gatgtcattt atatgtttgt gcatattgta agtattattg ttaggcacag actttttatc
    59581 acgtgctcta tctaaagaaa acttaacatc ttcagccgat acacgctctc cagtattacg
    59641 tgcttgtcca ttgaccactt tcgcaaaata atcatcatct cttaacaaga aataaaatgc
    59701 tttattgtcc ttattcacag cataatcatg acttaacgaa cctttcgttg ttaaatgatc
    59761 attttcatct aataataata accttgtgta catattcata ttaattgaat atactgacgg
    59821 cgcaattgaa cgtattggat ccaatgtagg aatttcacca tcttgttgtg tcatcacaag
    59881 tggccgcgta tctcgttctc tactattgtt gtaatcaaat tgttgccata ttaatgcacg
    59941 tgaatttggc aatccaacac tatttttatc taacacttta ttgtcatata ctaaattctt
    60001 ttttgatcca tataaaggcg ccatataccc tttatcaaat acaacttcat cttcaatttg
    60061 cttatatgtt tgtttaacat ctgcttcatt ttgagtagaa gctttattta acaactggtc
    60121 tacatgttta tctttcaata aactatttga tcctgtagaa ctaaataatg ccgtcatagc
    60181 atagttcggg tcaccaaaca ctgtcatcca gtcatcaatt tggatatcat aattgccggc
    60241 ttgacgttgt gtacgatagc taccataatc tggttggata ttcatcttca cgttaaatcc
    60301 tgcattttcc aattgatctt taacgatatt catatcattt tcataacttg cttgtcctag
    60361 gaaatgtatt gttggtcgct cgcctttcac ttcaactttc gatgactttt gagccacttc
    60421 tgatttcgta gggacaccac aaccacttaa taccaacgct aaaactataa ttgcgatact
    60481 aatgattttc ttcacatcta tccctacctt tttaatgaat tcttggatct agtgcatcac
    60541 gcactgcatc acctataaaa ttaaatgcta aaacgacgaa cataatacaa acaccaggta
    60601 caatagctaa attactgtgc gtttccaagt agttactacc ggtacgtaaa atgttgcccc
    60661 attcagctac atcaggtgca acaccaagtc ctaggaaact taaactactt gttgttaata
    60721 caaccacacc tatatttaat gaaaaacgta caatcatagg cgcaatcgca ttcggtaaaa
    60781 tataacgcca tatgatattc caagtgtttt caccagtgat acgtgctgca tctacatatt
    60841 ccatgcgttt aatttctaaa acactggcac gcattgtccg tgcaaatgat ggtatattac
    60901 cgatacttaa agcaataatt aaatttggaa tacttgctcc aaatgatgca ataattgcca
    60961 ccgctaacaa taatgatgga attgcaaaca ctacatctaa aattcgcatt attaaattat
    61021 caatatgatt aaaataacct gcgatagtgc ctagtaacac accaaaaata actgcaataa
    61081 ctactgaaat aattgaaatt gaaaatgtca gcttcgttcc tacaactacg cgtgtaaata
    61141 agtctctacc gaaatcatca gtaccaaacg gataggctag actcggtcca tgtaacagtg
    61201 cattgaactg atttttagta gccaatgtcg tatcaaatgt aaattgtgac acaattgata
    61261 atgtcagcat gtagactaaa ataagtaacc cgataatcgc aatacgatgt ctagtagttt
    61321 ttcgtataaa cgattcccac ccgttataac tatgtatttg cgatgtacgt tggtaacgtc
    61381 taatacttac aaacattaat aatgtaaata cgttgcctgt taatgtcatc aacaataaca
    61441 acacttcgac gatacgtcgc cataggtcat gatgcttcca tgtttgttcc gttgttaaaa
    61501 taataattaa aatgatggtt aaaacgatta gcaatgtttc agcaatatag aacgtatcgg
    61561 ccacataacc tttaaaaaga tttaatgcac tcgttaatat aactaaaata taagttgcta
    61621 tggcgtaact tgcgaataat tttaaggaag ctatctttga attaagttgt gccatatgcc
    61681 tcacttcctt tcgttgattt cactacgtaa ttttggatcg attaaagcat aaaatatatc
    61741 aataattaag tttgctaaag atattacaat tgatatatat acgaccccac ccatgactgc
    61801 tggaatatca ggtattagtt gtttttggac gatataacgc ccgataccat taatgttaaa
    61861 tacttgttcc gtcactgctg aaccgcctag taactctgcc actagaagac caactaacgt
    61921 tacaattgga ataatggcat ttttcaaaat atgtttaata acaacttgtg tcgtcgataa
    61981 tccttttgca taagcagtta aaacataatc gctgcgcatt acttcaagta cagaagacct
    62041 tgtcatacgc gtgatagaag cagcaatact tgttccaatg acaagtacag gtaaaatcaa
    62101 cgatattgga tgttctggca tataagatgg tggcaaaata tccaatttca atgagaacgc
    62161 taaaatgaat aatagccctt gccagaaact tggaatagat aaaccaatta atgcaattat
    62221 cattaacgtg atatcaagcc aactatttcg cttcatcgca ctgataatac caattggtat
    62281 tgcaataatt aatgccacca ttagcgctaa tactgcgaca attattgtaa ttggaattct
    62341 ttcgccaact gctttagtca caacctcatt ccctttgtaa gtcgtaccta agtcaaaggt
    62401 aaaaacaccc ttgatggtat cccacaattg aataaaataa ggttcgttaa gatgatgtaa
    62461 tacattgaat tgatgtatct gtgcctttgt tgcattttgt cccagtatgc tataagccgc
    62521 atcaagcggt gaaaaataca gaatggtaaa cacactgaca ataacaccaa tgatgacaat
    62581 cacagccatg acaattcgtt caaaaatata tctaactaat ggctgtaaat aaaaagtcaa
    62641 taagatgaac atcggcaagg ccaatatcac tttgatcatg atgaacttat gaaataatac
    62701 attttcaaag tatgttgaaa aatgtgcttg ttcaatattc tttgaactcg tattagaact
    62761 ttgtgccttg aatattttta atgcttcttt atgtatttgt gtggatgact tttgctgcga
    62821 taaatattta tatttttgat gtaacgcctg ttcaatttct gaaatttcag aattattagc
    62881 gtaaaaattt ttcctcttag cagaaaagaa aaactttatc actgcatata aaaatattgg
    62941 caagcttaat accgataata caaacttgta aaatgccttg tgatacaacg acttaaaaga
    63001 ttctaaataa gtaattatga taatcactcc ttgtaaaata atcttgagta gattactatg
    63061 atatacaaaa gtatagaata aatttacaca tttgtgaata gggaggcaca acatcatgtc
    63121 aaatttatta gaagtcaaca gtctgaatgt acaattcaat tatgatgaaa ctacagttca
    63181 agcggtaaaa aacgtctctt tcgaattacg aaaaaaacat atcctaggta ttgttggtga
    63241 atcaggatca ggaaaaagta ttaccgctaa atctatttta gggctactac cagattatcc
    63301 agatcacaca ttaacaggag aaattatttt taatgggcaa tcgttaaata atttatcaac
    63361 ttcagcgtta caacaaattc gaggtaagga tatttcaatg atttttcaag atccactctc
    63421 ttcgttgaat ccaagattaa cgattggcaa acaaattaca gaagtaatat ttcaacacaa
    63481 acgtgtatct aaatctgaag caaagtcgat gacaatagac attttagaaa aagtaggtat
    63541 aaaacatgca actcgacaat ttgatgctta tccacatgaa ctttctggtg gtatgcgtca
    63601 acgtgtcatg atagcaatgg cattgatttt aaagccacaa attttaatcg cagatgaacc
    63661 aacaacggca ttagatgcca gtacacaaaa tcaattactg cagttaatga agtcccttta
    63721 tgagtacaca gaaacatcta ttatttttat cactcacgat ttaggcgctg tgtatcaatt
    63781 ttgcgacgat gtgattgtaa tgaaagatgg aagtgtcgtt gaaagtggca cggttgaaag
    63841 tatttttaaa tcgccacaac atacctatac aaaacgctta atagatgcga ttcctgatat
    63901 tcatcaaacg cgtccgccaa gaccgttaaa caatgatatt ttattaaaat tcgatcgcgt
    63961 gagcgtggat tacacatcac cgagtggcag cctataccga gcagttaatg atattaactt
    64021 ggctattaga aaaggcgaaa cattaggcat tgtcggtgaa tcagggtcag ggaaatcgac
    64081 attagctaag acggtcgtcg gtctaaagga agtgtcagaa ggctttattt ggtataacga
    64141 attaccatta agtttattta aagatgatga attgaaatct ttacgacaag agatacaaat
    64201 gatttttcaa gatccattcg catctattaa tccaagattt aaagtcattg atgtgattaa
    64261 acgaccacta atcattcatg ggaaagtcaa agataatgat gacattatta aaactgtcgt
    64321 atcgttgtta gaaaaggttg gcctagatca aactttctta tatcgctatc cacacgaatt
    64381 atctggtggg caacgtcagc gtgtaagtat cgcgagagca cttgctgttg aacctaaagt
    64441 gattgtttgc gacgaggcag tgtccgcttt agacgtttca attcaaaaag atatcatcga
    64501 gttattaaaa caattacagt tagacttcgg catcacttat ttattcatca cacatgacat
    64561 gggtgttatc aatgaaatat gtgatcgcgt tgcagttatg aaaaatggcg aaatcgttga
    64621 actgaataac acagaagata ttatcaaaca tccgcagtca gactatgcaa agcaacttat
    64681 ttcagaagta gcagttattg ctaaataaaa gtcatgcgtt gtgcaacttt atcactgtat
    64741 ggtctgaaat aaattgcgcg acttctgatg tgttatgtta gatgactttg tcatactaac
    64801 tatattgtgc taagttaggg ctcggcacca tatcaagttt taggtgcttt gccatgattt
    64861 aagagtcacc cccatacttt gggcatttta acgccagaat aaatcccccg ccactatgtg
    64921 aagtgcgggg gattatttat attttattag aatattcaga tttttgagtg tgtcaactta
    64981 gcttagtcaa tgtatattta acgtcactta ctctttttct ttcataatta acacattcaa
    65041 ataaactttg atcaaaaaac acaaagttaa aagtaccatc ttgtaatatg ctctcataca
    65101 ttatcccgtc atatttaagg cttcgaatat aatcagctaa atattgaaat ggcaaataat
    65161 ctattccttg ttcatcgctt ggatttgtta ttcctttatg aatctttttt aatgtttggt
    65221 aatttacaaa atactttcta aatccatcat cgccagcttt gattgcatta ctagttaaat
    65281 tagttaaatt cgcaattttc aatttctctt ttgtcacgtt tttttgtaac ttaaccttac
    65341 ctatataaat aatgtcatta tgcttaggtt taacttcttc tatactgacc tgttcttttg
    65401 tactaaggta taatacgctt atccatttag aattcaatct tcctgccgtt gcaaatccct
    65461 ttggtggtga cattagttca cttttctctg taatgaactt aactattcta gatctatata
    65521 atggttcaaa tctttctcta aattcctcaa tactatagta attagtagtg atatcgagaa
    65581 agaacgctaa attctctaaa ttgatcatat tattatgaaa tctattttta tacttcaagc
    65641 tctcacaaaa tccatcccag tcattatttg ctacaattag atttttattt gtatattttt
    65701 tatcgtttat gattttagcg cctactaaat cttccaacac tcgtctatct aaattttcat
    65761 catctttaaa aagttcattt aaaatacaac ttatttgagc ttcctcaaca ttaaatatac
    65821 tccagtcgtc ttttaatgct atttcaatct ttttaccttc ttttgggcta aaagtatctg
    65881 gtaaatttat actaatatca tataattcta atgctggtct taaataatct ctaataagtt
    65941 ctaatttatc tatgtcctta gtcgtatcaa atattttaac accaagatga ttgttatcaa
    66001 tatcacaatt gtcaaatttg ctattatcat ttgcaatgat ttctacgatt tcagtattat
    66061 taaaacattt ttcacatatt ttcattttga gactccaagt atctattcat aatttctagg
    66121 tgatgcatga tagataacct tttaattaaa cctaatcctg gatacttatt attttcattt
    66181 aattcttcaa attgtccaag cgcataagat ctatttttaa tatctaagtt ttttgaccat
    66241 gttactaatt tatctacagc ctcaaaaaac tttttacctg gatttgatct atcattatta
    66301 gaatcagaga caaaatgctt aactcttagc tcattagcct catcaaaata gacaatatgt
    66361 atcgcaatag ctaatggcga aaagccaccg tctacatatt ctccacctat aacagaatag
    66421 tctgaaaatg ctacgtatcc atcttcctta taataaaggt gatcgtcgct ataaaactcg
    66481 tcatcattat ctaaataatc aacattacga cttagtttat taaaaggatc cataatgatg
    66541 cctttgtttg gaatattacg caatttacgt ttaatatgtc tatcatctgg tacaaaaacc
    66601 atttcaagtt tatttttatc tccaaacatt tcccaatcat ctacacttgt taaaatagct
    66661 aaactattac ttgaaaattg ctgtttcaac ctatcattgc cttcatcaga aggacccaag
    66721 taagcttttt gaataacact tttgtttttt gttattattt cagttaaatg tgcaacagtt
    66781 tcgttactaa tctcttcttc agttaacttt gaatttacta ctaaatttat agagaatgct
    66841 ttttcaccac agtattgcaa agtattttta aatgtggttg tatatttaat tggttcaatt
    66901 ataggttgaa tacaatcacc aatcaaacct ttctctaaca attctctaat tgcaaatagt
    66961 tcattttgtt tcccacgcaa ataaggataa tacatattaa tcctccttaa gaaaatgata
    67021 tagtttattt ttatctttct ttgtcatttt tgcgaaatat aacaagaact taatttcagc
    67081 cggcgctgct aaaaattcat ctttgtattc tttcatgttt cctctttttt tcaactcaga
    67141 aatcatattg ttatgcagtt tcgtcattgg tatttcttta aacacgtcaa ataacacatc
    67201 atactctgtg aaatcattat aagttggcac atccatatga ttttgtgcaa taactctttt
    67261 tctttcttct tttcgtaaaa agttgtacat cgcttttgtg tttaagagac tattgttttc
    67321 tttaggtttt tgaacttcac tcagtgtatt tctagttgtt aacactaaaa ttccaactgt
    67381 tgtatctttg tatctagcca taactttatt cagatgtttg tcatttgtaa taactacgac
    67441 ataattaaac acttcataat aatcattaat ttgattatct aatctatcca gcttatctaa
    67501 ttctgtttta atctcataga caacgccttt gccgtttaac aatataaaat cagcaatact
    67561 tttccctatg ggcatctcag aaagtgcagt agttgtatta atagaatgtc gtcctagaag
    67621 gagtttatta agtattgtgt ttttgtaaaa atattcgtta cgataagact ttttaagata
    67681 gttatataaa gatttcatct tctgtattac attgatattt ttaggtccct gtatataaaa
    67741 atcatatgcc tcatcaacaa catcactttt atttttgatt gcaatttgat gtaatatctt
    67801 ttgactaaaa acacgattaa tcatatttct attatccata gctacgcacc tccttcaaca
    67861 tacatttaat acttatcctg ttaatattat aaactatttt aaatgcctat agacattatt
    67921 tctatacaca tacgattata aaattgtatt gaaaccaaat gatggttgcc aaacacatgg
    67981 taactaacaa aagatagaaa ataataatgc cccctacact aatctagcga gggggctgaa
    68041 tcattacaca ccgtattttt cagttgtttc tgccacgaaa gattcaatgg cttttcttgc
    68101 tttacgcttt tctttcaatg gcaaatcacc aatcattttt ttaaggtgat gtgggtttac
    68161 aacaccacta tactggtagt catttgaaat tgtttttagg gcttgttcat cgatagatct
    68221 ctctccagca aatcctttga actccgcttc ttttttaata ctttcgaaat taacatattc
    68281 ttgatcgata tcatcatcct tatttaaaga aggtacaaca ttgtcgatga attctctaat
    68341 tagatctcgt tttaacctca atgtcggatc atctgcatga tctaaaatgc gtctaatttg
    68401 ttcttggtta cgacgttgtt ccgctttgtc ttcaagatca atttgtctca atatattcat
    68461 aatataattc acattaatcg tatcattacg catcatttct atttcgaaat caatatcatt
    68521 taaaatggat actttatttt tctcagccgt cgctcttttt acttgatcgt acacagctaa
    68581 atatttactt ttatagtctt cattctcttg ttcatccatt ccaatttcat caattgtaaa
    68641 ctcaaactcg tcaaatgctt ttaaacgtaa tattatttta gctaataaac gataagcttc
    68701 aacaaagcgc tttagctctt cttcatcttg aatgtcatca accatgtgtg gtgtcggcac
    68761 aatcatttta agctcacgat aagcgtccat aaattctttt ttatactctt cataactgcg
    68821 cattaaaatt gtatccgtat catttgtttg tgagaatact ctcagtgcat cgtctgtctc
    68881 ttttttcaag tcacgatagt ttacaatttt accaaatggc tttgattctt tttcaaccct
    68941 atttgtacgt gaatacgctt gaattaaatc atgatacatt aaattcttat caacatataa
    69001 agtgttcagt actttactat caaaaccagt taagaacata ttaacaacga ttaagatatc
    69061 aattttacta tctttaacgc cctttttaac gttttttgaa atatgattaa aatactcatt
    69121 agttgtgtct gttgaaaaat tcgtctcgaa ctttttatta taatcactaa tcattatctc
    69181 taatttttca cgtgaatgat atggcacttc accatcacga tcatcttcat taggtttaaa
    69241 cgtaaatata ccagctatcg ttaacggttg ttccaacttt ttgttaagtc gcttaaatgt
    69301 ctcataatat ttaataagcg cgtgaatact ttggactgta aatatacttg aatattgacg
    69361 attacgtgta tatttatcat gattattgat gatatgtcgt gttactaatt ccacacgttt
    69421 atccgctaac catacttctt ccgtatcaat tgcttcaacc atgctgttat cttctgcttt
    69481 taaagcttta tttttaaaag tattaatata gtcaactgag aaaccaagta cattaccatc
    69541 atgaatggca tctctaatta aatacgtatg taagcatcta ccgaaaatat ctgcagttgt
    69601 tctaccatct tgactactat tttctggaaa acgtggcgta ccagtgaatc caaagtattg
    69661 ggcatttttg aaatgttgtt taactagacg atgcatgtca ccaaattgac tgcgatgaca
    69721 ctcatcaata ataaatacaa ctttattcgt tttatactgt tctaataaag gggcattccc
    69781 ttgaatcgct ttagccattt tttgaatcgt cgttacaata agtggcaaac ttttatcatt
    69841 tagttggcgt accagttgcg aggtattaaa agttttgtct acagcaccct tagcaaattt
    69901 attaaattcc tcttctgttt gactatccaa gtctttacgg tcaaccaaaa agataacttt
    69961 cttaatgtca tcttgctgtg ataaaatctg actcgcttta aaagaagtca acgtcttacc
    70021 acttccagtt gtatgccata catatccatt attccctgtc tcagtcgctt gttgaataag
    70081 tgcttctacc gcatacactt gatacggacg cattgccatc agtattctat ctgtttcatt
    70141 aataatcata tagcgcgata tcatcttagc taattgacaa ggtctcataa atgactcagc
    70201 aaacgattgc aatgtattga tacggttatt ctgtttatca ctccaataaa acatgtgact
    70261 cttcaatagt tcgctatcat tattagaaaa gtatcgcgtt tcaacaccat tactaatgat
    70321 aaacatttgt atgtagcgga ataagcctgt gtaattttgt ttgcggtaac gttttacttg
    70381 gttaaacgcc tcattaatat caatacctcg acgtttcaat tcaacttgga caaggggtag
    70441 tccgttgatt aatatcgtta catcataacg tgctttatat gtatcctcga cagatacttg
    70501 attcgtcact tgaaacttat ttttacacca acttttcgta tctaaaaacg acaaataaat
    70561 ctcagactca tcatcacgtc taagtggtaa tttatcacgt aaaatacggg cactctcgaa
    70621 aatacttttt ccatcaatca tcgttaacag acgttgaaat tctttatctg ttaagggatt
    70681 gccttctaat ttgtccgcat gacgctcatt taaaatcgtt ctaaaattat caagcaattg
    70741 cttattatca cgtatcgtta ctctttcgta acccaattgt tcaagttgat tcatcatttc
    70801 attttctaat gcgtattcac tttggtatgc cattcatatc cccttccata cactttctat
    70861 tgctctaaat atatcataaa ctttaatgaa aaatgtttgt tttttatctt caaacgtaaa
    70921 tttattctaa ttttattgtc ttatctttta atatttgtct ttgaggtaag tcgtatacta
    70981 aaatttgaat acaaataatc aaatcattga taaatttttt gtctacgatt aatggaggga
    71041 cttgaatggt gttaattacc tatcaaatca ttttattttt tattattagt ctaagttact
    71101 atttaacttt aaatcattac atggcagtca ctgtaggtaa cttcacttca atattcggca
    71161 tgttcgcagc catactcttt atgtactact acctactcta taaaagtccc gaatacaatc
    71221 aacgcaaacg atttaaacat ttcattcata tcactaattt gataataatt gcttttagca
    71281 ccttcgtatt agttcattta gcattaaaat tattcttcag catttaattt ccatctatga
    71341 aaaaagcaaa gctcaaatct gaactttgct ttaatttgtc acgcctttat cattttcaaa
    71401 atagcctcta tgccagtttt acaaacttgt agcaacaatt tttcatcaag caactgaatc
    71461 acatcaaaaa cttcaattgt ttacgttgca actaacttta tgttagaacg cacacaatat
    71521 ctaatgttta aaatctatcg tcgcaagatg cttcctgtaa ttatcaagtg ccattttcga
    71581 ttgggttata caatctagaa tcgcatgata atttaatgct acaaatcgat agtacaatat
    71641 atctaccgtg aataactgtg caaatagtga cgttgtagcc gccatacgca tttcattttc
    71701 atcagttctg ccataaatca atgcatagtc tgcaatttga gccactggat tattagctgt
    71761 actagatata gttatgatgg gaatactgta atgtgtggcc acctgtgcaa ttgactgcaa
    71821 ttcactatga ctaccttgat tcgtcacaaa aatcatgcaa tctctatcat catgcgtcgc
    71881 aaatgttgac acaagtaaat gcgtttcatg taataacctg acatttaagc caatacgaga
    71941 taacttttga aaaagatcac caatagtcaa actcgatgcg ccaaatccaa ataaaaatat
    72001 tgtcctggca tttttcaaca catcacaaat tgcatcaatt tgcgcatcca taatattagt
    72061 agctacaaat cgcatcgtat tcgttgctct agcaatcatt ttatttttca aagtttctac
    72121 agattcattt tcaatcaatt ctaaatgtgg attggttgca atatcttcgg gtaagtatcg
    72181 agatatcgca atctttagct cttgaaaacc ttgatgtgtc attttccgac taaatctaac
    72241 aattgatgct gtactaacat tcgtaacatc tgccaaatca ttcacagtca tatcaatgat
    72301 tttatgtgga ttctttaaaa tgtaatcagc gattatcttt tctgtcttcg taaaatcact
    72361 caactgctta tcaatgcgat ataaaatatt tgtcatcatt aatcacccaa caaatctgtc
    72421 tgtcgcatcg cctttgtcgt tccaaataaa tatgtacaaa cgaatccacc agcatacgca
    72481 gcaagtaatc ctgcaatata acctaaatac atattatctg agattaatgg taatagtgac
    72541 acaccacttg ggcctattgc tttggcacca atatgtccaa ttccacctat tacagcgcca
    72601 ccaataccac caccaataca agcagttaag aaaggtcgac ctaatggcaa agtcacacca
    72661 tagattaatg gttctccgat acctaggaaa ccaactggca atgcaccttt taaagtatta
    72721 cgtaatgttg tgttgcgttt acatcttacc caaagtgcta atgcggcacc tacttgtcca
    72781 gcaccagcca tcgctgcaat tggcaataag taagtagcac ctgattggtt aatcatttct
    72841 atatgaattg gcgtaaaaat atgatgaagc cctaacataa ctaacggtag gaagcttgca
    72901 ccaatgataa atccactaaa tacgccacca atactaataa ttccgttaac tactgaaact
    72961 aaactgtctg aaacaaaacc tgctaatggc ataaagataa agatagttaa tagtcctaca
    73021 atcaacaatg caatagtcgg cgttacaata atatcaatcg catttggcac aattttatgt
    73081 aatctctttt cgacaatact taaaatccaa acggcaaaaa taacgccaat aatcccacct
    73141 tgtccaggtt gcaatggttc tccagtgaag acattcatta aaatattttt accagcaata
    73201 cccgttaata acgttgtacc accaatcacg ccaccaagtc ctggtgtcgc accaaattct
    73261 ttagccgcat taataccagt gaaaatagct aagtatgcta acataccgtc tttaatgaca
    73321 ttaaatactg ttataagttg cgtaatccaa gcacctgaaa tatagcctgc caccattaag
    73381 ttactcagta ctgctgcaat accaccaatt aatccagctc caataaatgc aggaatcaac
    73441 ggtataaaga tattggcaat tgatttcaat actttattca acttaccatt cttttgtttt
    73501 gctttatgcg cttccttatt cgcctttgct ttatcagctg catatgattt atagtccatt
    73561 ttttcactat cattgtgatg gtgtggtatt gggtcaccta gtttaacacc acttaattcc
    73621 gccatatgat tagccacttt attgactgta ccaggtccaa ccacaacttg aatgcgttca
    73681 tcgtgtataa cacccatgac accatcaata tgccttagtt cttggtcatc tactttattc
    73741 tcatctaata ctttaatacg cacacgtgtc atacagttca tgacactatc tatattatcc
    73801 ataccaccta ctgcagcaat aattcgttct gcaagttgtt gttctttggt catttaaatc
    73861 cctcctaagg ttgtctatct ctgattgctc gtttaacaat gtcaccattg tttaataacc
    73921 gtcttgttgc ttcttcctta gaaatgccac acatacccat aactgtcgca actttcacat
    73981 catgctcaga tacctgatat aacgccattg cttcatcata tgtgatagca catatttctt
    74041 gaataatacg cactgaacgg tcgatcagtt tttgattggt tgctttaaca tcaatcatga
    74101 ggttatcgta aacttttccg acaccaacca ttgtgatggt tgaaatcata tttaaaatta
    74161 acttttgtgc tgtaccagac tttaaacgtg ttgaaccagt taatacttct ggaccaactt
    74221 taacttctac tggatactgc gcaatttcac ttataactgc atgttcattg catgaaatag
    74281 atactgttgt agcaccgatt gtgttagcaa atgttaaacc gcctataaca tatggcgttt
    74341 tgccactcgc ggcaattcct ataacgacat cttttgatgt taaatctata tttttcaaat
    74401 cttcttccgc taattttttg tgatcttccg caccttctac agccatcgtc atagcatgtt
    74461 gtccaccagc aataatacct ataatttcat gagggtcagt attgaatgta ggtacacact
    74521 ccgctgcatc taagacaccc aaccttccac ttgtacctgc accgatataa atcaatcgtc
    74581 cacccttttt atactgtgca attgtttttt taattacttt tgtcaattgt ggtattgcct
    74641 ttcgaactgc taacgggact tgctgatctt ctttattcat cgtaattaaa gcctcttcca
    74701 cagtcatttc atcaagatgc atcgtcgctt cattacgcgc ttcggtcgta ctattttcca
    74761 tcacttctta cactccctag ttttttgaaa atcaaatgta tcattcggct cgatacaact
    74821 taacagtggt aagtcttctt taataatttg tgcaacaaca ttcacattgt catgtgcact
    74881 aagcgtttgt ctcacaattt gcatttcgcc ttgataacgt ccgttattca aattatcaac
    74941 ggttactgaa ccaatgcgtc gttgcgtcgt aaactgtggt tgaatcgaat gtggacatat
    75001 ttgtcttgac gtttccgaac gaatgacatt ttccggatta tccgggcgta ctttatgaca
    75061 catatcgaaa aggtaagtca ctgtcgtatc aaacacttct tcaatacata acgtgaaatg
    75121 cctatgcttg caaaaatcta taagttgttt tgcctgcctc atttcaatca atgagtctcc
    75181 aactaacact tcagatacac cagtttcttg taataattta gctgcaacga caggatgact
    75241 atgtctcgtt gcttcaattg ttggcaagcc tttatgcaaa ggacctcgca aatcactccc
    75301 tacaataaaa ccatatattt gtgcctttgg attaaattga taaatgagtt catttttctt
    75361 attgaccaag tcaacagata atcccgtatc tggtcttgga taatagttat gacaaaatga
    75421 aagtaatgta aaatcattca attgttgatg taagcttgtt aacaattccc gggaaataat
    75481 acttgcattc aaacagcact ttaaaccctg tgccattatc gcttcgattg cctcaattga
    75541 tgtactatga tcgatacgaa tcataaattg tgcatcatat tgtcgaagat ggtcataaaa
    75601 agatggtgtt aaaatagatg gattagcatc tatgaggtaa gtcacttgtt catgttttaa
    75661 taaattgagt agttttgtga aataatgata ttttgtctcg tcatcttctt ctggtatttg
    75721 tacagatgta aaaatcattt ggtaaccttg tttaatcatt cgcttaatat acgcttcatc
    75781 taaaggttgt cctaaataca ctgaaaagcc tgtcaaagta gccctcctta acaatataat
    75841 tattaggaaa atatagttga gttgtgtaat cgcttacatt ttactataag agaaaacaca
    75901 ttacaatatt aatcagttaa agcctgttca ttgtaataat cttacatatt tctgtcacaa
    75961 gttaattatt acaccatcaa agattatcct ttcttttaag tgctgataat agctgctact
    76021 gctggattat tacaataact tttatacatt ttattcagga ttatcttata ttatgtttta
    76081 ataataatct gtgaacaatt aagagatttg aaattgaatt taataattgt attgaaaacg
    76141 catacttcac catgctaaaa taggagtcgc aaacaaataa gattcaataa gatgtgatgg
    76201 taagctagat aaactaacgt atcagtttta tatactactt agtcaattgc ttcttttaca
    76261 ttaattacat acaccaacgt gttactaagt aagattaggc atgagttaaa ttaagctgtg
    76321 atggttacca acacagtcta tttgctcgtg tcttttttta ttgaatctta aataataaat
    76381 acaactttgg aggttggaca agtgaggaag aaacttttcg gtcaattgca acgtattggt
    76441 aaagcgctaa tgttacctgt tgcgatttta ccagcagctg gtctgttatt agctatcggt
    76501 acagctatgc aaggtgaatc attacaacac tacttgccgt ttatacaaaa tggtggcgta
    76561 caaactgtcg ctaaattaat gacaggtgct ggtggtatca tttttgataa cttgcctatg
    76621 attttcgcat taggtgtcgc aatcggatta gctggcggtg atggcgtagc agctatcgca
    76681 gcattcgtcg gttacataat catgaacaaa acaatgggcg actttttaca agttacacct
    76741 aagaatattg gtgatccagc gagtggttac gctagcattt taggtatccc aacattacaa
    76801 acaggtgtgt tcggcggtat tataatcggg gccctggcag cttggtgtta taacaagttc
    76861 tataacatta acttaccatc ttatttaggt ttcttcgctg gtaagcgttt cgtacctatt
    76921 atgatggcta caacatcatt tattttagca ttcccaatgg cattaatttg gccaacgatt
    76981 caatcaggat taaatgcatt cagtacagga ttattagatt caaatactgg tgttgccgta
    77041 ttcttatttg gtttcatcaa gcgtttatta attccattcg gtctacatca cattttccac
    77101 gcaccgttct ggttcgagtt tggttcatgg aaaaatgcag ctggtgaaat tattcacggt
    77161 gaccaacgta tctttatcga acaaattcgt gaaggcgcac atttgacagc tggtaaattc
    77221 atgcaaggtg aattccctgt tatgatgttc ggtttacctg cagcagcttt agcaatttat
    77281 cacacagcta aacctgaaaa taagaaagta gtagcaggtt taatgggttc tgctgcttta
    77341 acatcattct taactggtat tacagaacca ttagaattct cattcttatt tgtagcacca
    77401 ttattattct ttattcacgc agtacttgat ggtttatcat tcttaacatt gtacttatta
    77461 gatcttcatc taggttatac attctcaggt ggtttcatcg actacttctt actcggtata
    77521 ctacctaata agacacaatg gtggttagtc attcctgtag gtcttgtata cgcagttatt
    77581 tactacttcg tattccgatt cttaattgta aaattaaaat acaaaacacc aggtcgtgaa
    77641 gataaacaat cacaagcggc tactgcttca gcaactgaat taccatatgc agtattagaa
    77701 gctatgggtg gcaaagcaaa cattaaacat ttagacgctt gtatcacacg tctacgtgtt
    77761 gaagttaacg acaaatctaa agttgatgtt cctggtttga aagatttagg cgcatctggt
    77821 gtattagaag tcggcaataa tatgcaagca atttttggtc ctaaatctga ccaaatcaaa
    77881 catgaaatgc aacagattat gaatggtcaa gtagtagaaa atcctactac tatggaagac
    77941 gataaagacg aaactgttgt tgttgcagaa gataaatctg caacaagcga attgagccat
    78001 atcgtgcatg caccattaac tggtgaagta acaccattat cagaagtgcc tgatcaagtg
    78061 ttcagcgaaa aaatgatggg tgacggtatc gctatcaaac cttcacaagg tgaagttcgt
    78121 gcaccattca acggtaaagt acaaatgatt ttcccaacaa aacatgcaat tggtcttgta
    78181 tcagatagtg gtttagaact attaatccac atcggtttag acactgttaa attaaacgga
    78241 gaaggcttta ctttacatgt tgaggaaggt caagaagtta aacaaggtga tttattaatc
    78301 aactttgatt tagactacat ccgcaatcat gcaaagagtg atattacgcc tattatcgtg
    78361 acacaaggaa acattacaaa ccttgatttt aaacaaggtg aacatggcaa catttcattt
    78421 ggcgatcaat tatttgaagc taaataatgc ttactataaa caggtgcgta taccttcata
    78481 aggtgacgcg cctgtttttt ctttgctatt gtattttgca gcatcattga tagttcgctc
    78541 tccccttaaa ttttgaattt taagatcatc aattaaagcc ccccttcata ctcatttcct
    78601 aaaaaatatt aattgttcac tattgttagc gttttcacaa caaagtcaac ttccttgacc
    78661 ttacactata ttcgaggcta tcattttaag tgtaaatata gagaaaaggt ggcttttttt
    78721 atgaaacaac gcattggagc ttacttaatt gacgctattc atcgagcagg cgtcgataaa
    78781 atttttggtg ttcctggtga ttttaatctc gcttttctag acgatattat cagcaatccc
    78841 aatgtagatt gggtaggaaa tacaaatgaa ttaaacgcaa gttacgcagc ggacggttat
    78901 gcccgtctta atggactcgc tgcattagtt actacatttg gtgttggcga attaagtgcc
    78961 gtcaacggta tcgcaggttc atatgctgaa cgcatacctg tcattgcgat tacaggtgcg
    79021 ccgacacgtg ctgttgaaca aggcggtaaa tatgtacatc actcacttgg tgaaggtaca
    79081 tttgacgact atcgaaaaat gtttgcacat ataaccgttg cacaaggtta tatcacacct
    79141 gaaaatgcaa caaccgaaat accacgttta attaatacag caatcgccga aagacgccca
    79201 gttcatttac atttaccaat cgatgtcgca atctctgaaa ttgagatacc gacaccattt
    79261 gaagtgacgg caactaaata tacggatgca tcaacatata tagagttatt agcaactaaa
    79321 ctgcatcaag cgaagcagcc tatcatcatt actggacatg aaattaacag ttttcacctc
    79381 catcaagaat tagaagattt tgtaaatcaa acacagatac cagtagcaca actttcatta
    79441 ggaaaaggtg cttttaatga ggaaaatcca tattatatgg gtatttacga tgggaaaatt
    79501 gccgaagata aaatacgaga ttatgtggac aacagcgatt taattttaaa tattggagcc
    79561 aaattaacag attcagcaac agcaggtttt tcataccaat tcaatatcga tgatgtcgtt
    79621 atgttaaatc atcacaatat caaaattgac gatgttacaa atgatgaaat atctctacca
    79681 tcattgttaa aacagttatc caatatttca tatacgaata acgcaacgtt ccctgcgtat
    79741 catcgtccaa catcacccga ttatactgtt ggcacagaac cattaacaca acaaacttat
    79801 tttaaaatga tgcaaaattt cttaaaacca aatgatgtca tcattgctga tcaaggtaca
    79861 tcattctttg gtgcttatga tttagcatta tacaaaaaca atacttttat agggcaaccg
    79921 ttatggggtt ctatcggcta tacattacct gcaacattag gttcacaatt agcagacaaa
    79981 gatcgtcgta acttattatt aattggtgat ggctcattgc aactaactgt tcaagctatt
    80041 tcaactatga ttagacagca tattaaaccg gtattatttg tgattaataa tgacggctat
    80101 acggtagaac gacttattca cggcatgtat gaaccttata atgaaattca catgtgggat
    80161 tataaagctt taccagctgt atttggtggt aaaaatgttg aaattcatga cgttgaatca
    80221 tcaaaagatt tacaagacac gtttaatgca attaatggtc atcccgatgt gatgcatttt
    80281 gtcgaagtca aaatggctgt cgaagacgca ccgaagaaac tcatcgatat cgctaaagct
    80341 ttttcacaac aaaataaata atttcatcgt atacagggta taagtttaag cgaatacttt
    80401 attaaacgaa taggactctg atataagatg attaatttta ataaaaccgc tttagtgtta
    80461 atcgacctgc aagaaggtat tcttaaaatg gattatgccc catatacagc tgaaaatgtc
    80521 gttcaaaacg ctaataaatt aatagatgtt tttagaaaaa acaatggctt tatcgctttt
    80581 gttcgcgtga atttctatga tggtaaagat gtattgcaac caaatgcaat gatctcatta
    80641 ccaccaaaag aaggcgacga ctacagtcgt ttccatcatt tattagacaa gagagatgac
    80701 gattttgtca tagacaaacg acattttagt gcatttgtag gaacagattt ggacttacaa
    80761 ttgcgacgtc gaggaattga tacgattgtt cttggtggtg tcgcaacgca tattggcgta
    80821 gatacgacag cgcgagatgc ctatcaatta aactacaatc agttttttgt tacagatatg
    80881 atgagtgcac aaaacgaaac gctacatcaa tttccaatag ataatgtatt cccattgatg
    80941 ggacaaacaa taactacaaa cgactttcta aatatattga actaaacata tacttccccc
    81001 cttcgatcat gttgaggggg atctttattt cacaaagtat taatacgtcg ggttgtctaa
    81061 ccttctatat ttaacatatt ctatatctgt taaatcgttc ttaacttacg cccctactac
    81121 ataaaaaaca gtatttattc cggaattttc aaaaaattta gtatttattg caaaattatg
    81181 tatcacttta tgtttaattt ttgatattat cttaattaag tagattttta taagttctaa
    81241 aaaggagaac aaatacatat atgaagaaga aactaacatt taaagaaaac atgtttatag
    81301 gttctatgtt atttggttta ttctttggtg ccggcaatct tatcttccca atacacttgg
    81361 gtcaagctgc tggttctaac gtttttatcg ctaacttagg atttttaatt acagcaattg
    81421 gcttaccatt tctaggtatc attgctattg gcatttcaaa gacatctggt ttatttgaaa
    81481 ttgcatcgcg tgttaataaa acatatgctt acattttcac gattgcctta tatctagtta
    81541 tcggaccatt tttcgcctta cctagactgg caacgacatc atttgaaatt gcattttcgc
    81601 catttttatc accaaagcaa atcactttat atttatttat ttttagcttc gtcttctttg
    81661 tgattgcatg gttttttgcg agaaagccat caagaatttt agaatatatc ggtaaatttt
    81721 taaatccggt attcttagta ttattagcaa ttattttatt atttgctttt atccatccat
    81781 taggtggcat atctgatgca cctattagta aacaatatca atcacatgcc ttatttaacg
    81841 gctttttaga tggatacaat accttagatg cgctagcgtc attggcattt ggtattatca
    81901 ttgttgcaac gattaaaaag ttaggtatcg aaaatccaac tgatatcgct aaagaaacaa
    81961 ttaagtctgg tactatcagt atcattatga tggggatcat ttatacccta ctagcaatca
    82021 tgggtacatt aagtattggt catttcaaac ttagtgaaaa tggtggtatt gccttagcgc
    82081 aaattactca atactactta ggtaactacg gtatcgtcct gttgtcactt atcgttatgg
    82141 ttgcttgttt aaaaacagcc atcggtttga ttacggcatt ttcagaaaca ttcgaacacc
    82201 ttttccctaa aatgaattac ctagcgattg caacagttgt aagctttatt tcgttcttat
    82261 tcgcgaatgt tggtttaact aagattatta tgtactcagt cccagtgtta atgttcttat
    82321 atccattagc aattgccttg attgtactaa cattatttag tagcaaattc catcattcaa
    82381 aacttattta tcaatgtacc attttcttta caatgattgc tgcattagta gatggattaa
    82441 aagctagtcc agagttcatt tcaagcacat cattctcaca aactttgatt aatttcagcc
    82501 aaaaatattt accattatca gacattggta tgggctgggt tgttctcagt ttgattggtt
    82561 tcattatcgg cttcattatt tataaaatta agcatcgtaa aattccacaa gcataatact
    82621 atgccacagt catatgttaa acatatgctt gtggcatttt ttattcatac tacattaaac
    82681 tgcaatcgta tacatacata tcaatgatta tccacaaaaa atattagtac tttcatttta
    82741 caaatcacat taatacaaac acaaccttat ctttatatta ttaaatttac atttgacact
    82801 tatattgaac aactgtaata tattaatatt aattctttaa aatgtataaa tataaaggag
    82861 ggagaccgat gaattcaatc attgaattaa ctgattatta tagctctaat aattatgcac
    82921 cacttaagct tgtcatttct aaaggtaaag gtgtcaaagt ttgggatact gatggcaaac
    82981 aatatataga ttgcatttcg ggtttttcag ttgcaaacca aggccattgt catccaacaa
    83041 ttgttaaagc gatgacagaa caagcttcaa agttgtctat catttcacgt gtcctttata
    83101 gtgacaatct cgggaaatgg gaagaaaaaa tttgtcatct tgctaagaaa gacaaagtac
    83161 tcccccttaa ctctggtact gaagctgttg aagcagccat taaaattgct agaaaatggg
    83221 gctctgaagt taaaggcatt actgacggac aagttgaaat catcgctatg aataacaatt
    83281 ttcacggtcg tacacttggc tcattatcac tatctaacca cgacgcatat aaagcaggat
    83341 ttcaccccct acttcaaggc actacaacag tagattttgg agacattgaa caattaacac
    83401 aagctatttc accgaataca gcagcaatta ttttggaacc aattcaaggt gaaggtggcg
    83461 ttaatatacc accgaaagga tatattcaag ctgtgcgtca actatgtgat aaacatcaaa
    83521 tattattgat tgcagatgaa attcaagttg gtcttggtag aactgggaaa tggtttgcta
    83581 tggaatggga gcaagtcgtt ccagacattt atattttagg taaggcattg ggtggcggct
    83641 tataccctgt atctgctgta cttgcaaata atgatgtcat gcgtgttcta acaccaggta
    83701 cacatggttc aacatttggt ggtaaccctt tagccattgc aatatcgacg gcagcgcttg
    83761 atgtacttaa agatgaacaa ctggttgaac gatcagaacg cttaggttca tttttattaa
    83821 aagcgttgct acaacttaaa catcctagta ttaaagaaat tagaggtcgt ggtttattta
    83881 taggcataga gcttaacaca gatgctgcac cttttgtgga tcaactgatt caacgtggaa
    83941 tcttatgcaa agacacgcat cgtactatca ttcgattgtc tccacctcta gtcattgata
    84001 aagaggaaat ccatcaaatt gttgcagctt ttcaagacgt ttttaaaaat taacaattaa
    84061 tcatttatat atgacatagg agggattcat gatgattaaa gtaggtatcg ttggcggtag
    84121 cggttatggc gcaattgaat taattcgatt gttacaaaca catcctcatg taacgattgc
    84181 acacatctac tcacattcaa aagtagatga accgttgaaa ttaacatttc cacatttaca
    84241 acatattatg caacatttcg aagcacttac agtggacaat aatgactgtg atgtaatttt
    84301 ctttgcgaca ccagcacccg taagtaaaac atgtatccct cccttagtag aaaaaggtat
    84361 tcatgttatc gatttatctg gcgcatttag aattaagaat cgtgaaatat atgaagcata
    84421 ttacaaagaa actgctgcag cacaagatga tttgaatcat gctatttaca gcatttcaga
    84481 atggcaatcg tttgataaca atggaacgaa gctcatttct aatcctggct gtttccctac
    84541 agcaacatta ttagcattac atccacttat tagcgaaaaa atagtagatt tgtcatctat
    84601 tattattgat gctaagaccg gcgtgtcagg tgctggtcgt tcattatcac aacgtgttca
    84661 tttttcagaa atgaatgaaa atctaagcgc ttatgcaatc ggaaaccata aacacaaacc
    84721 ggaaatcgag caatatttat ctatcattgc gggtcaagat gtatcagtca tatttacacc
    84781 acatctcgta ccaatgacac gaggtatttt atcaacaata tatgtcaaat tatcatctga
    84841 atatacgact gaatcattac ataaattaat gacctcttat tatgctaatc agccatttgt
    84901 cagaattaga gatattggga cttttccaac cacaaaagaa gtactcggta gtaactactg
    84961 cgatatcggc atctatgtag atgaaacaac gcaaacagca attttagtat cagtgattga
    85021 taaccttgtc aaaggcgcaa gtgggcaagc cattcaaaat ttaaatatat tatatgattt
    85081 tgaagtgacg actggcctaa atcaatcacc agtttatcca taaggggtgt tagaatgaaa
    85141 catcaagaaa cgacatcaca acaatataac ttttcaatta ttaaacatgg cgatatcagt
    85201 acacctcaag gcttcacggc tggtggtatg cacatcggtt tacgcgctaa caaaaaagac
    85261 tttggatgga tttactcatc gtctttggca agtgcagctg ccgtatatac tttaaatcag
    85321 tttaaagctg caccacttat tgtcactgaa gacactttac aaaagtctaa aggaaaatta
    85381 caagcacttg ttgttaattc agctaatgca aattcttgta ccggtcaaca aggcatagat
    85441 gatgcacgac aaacacaaac atgggttgct caacaacttc aaataccatc tgagcatgtt
    85501 gctgttgctt caactggggt cattggtgaa tatttgccta tggataaaat taagactggg
    85561 accgaacata ttaaggatgc taattttgca acgccaggtg cgtttaacga ggcaatttta
    85621 acaactgata cctgtacaaa acatatcgct gtatcactaa aaatcgatgg taaaaccgtt
    85681 acaattggtg gtagcaccaa aggttcaggt atgattcacc caaatatggc taccatgctt
    85741 gcttttataa caaccgatgc atcgattgaa tcgaatacac ttcatcaatt attaaaatct
    85801 tcgactgacc atacatttaa tatgattact gttgatggcg atacaagtac aaatgacatg
    85861 gtattagtca tggcaaatca ccaagttgaa caccaaatac ttagtcaaga ccatccacaa
    85921 tgggaaacat ttgttgatgc attcaatttt gtctgtacat ttttagctaa agctatagcc
    85981 agagatggcg aaggcgcaac aaagttaatc agcgtcaatg tgtcaggcgc taaatcaata
    86041 tcagatgcaa gaaaaatcgg taaaactatc gtaagttcaa atctagtaaa atcagctatt
    86101 tttggcgaag atgccaattt tggtcgaatc attacagcta ttggctacag cggatgtgaa
    86161 attgatccta actgcacata tgttcaactg aaccaaatac ctgtcgttga taaaggtatg
    86221 gctgtactat ttgatgagca agctatgtcg aatacattaa ctcatgaaaa tgtcacaatt
    86281 gacgttcagc ttggtttagg taacgctgca gcgactgcat acggttgtga tttatcctat
    86341 gattatgtgc gtatcaacgc atcatatcga acataaggtg gtgttggtta gatgaaattt
    86401 attgtcatta aaattggtgg cagtacactt agtgacatgc atccatcaat tattaacaac
    86461 attaagcatt tacgatcaaa caacatctac cccattatcg ttcatggcgg tggcccattt
    86521 attaatgaag cattatcaaa ccagcaaatc gagccacact ttgttaatgg cctaagagtg
    86581 actgataaag caaccatgac cattactaaa cacacgctca ttgcagacgt taacactgca
    86641 ttagtagctc aatttaacca gcaccaatgt tctgcaatag gcttatgtgg tttggatgca
    86701 cagctgtttg aaattacatc ttttgatcaa caatatggat atgtcggtgt tccgaccgct
    86761 ttaaataagg atgctttaca gtatttatgt actaaatttg tacctatcat caattcgatt
    86821 ggtttcaata accatgatgg agaattttac aatattaatg ctgacacgct tgcctatttt
    86881 attgcatcat cattaaaagc gcctatttat gtattaagta atattgcagg tgtactcatc
    86941 aatgatgttg ttatacctca attgccatta gtcgatattc atcaatatat tgaacatggt
    87001 gatatttatg gaggtatgat tcccaaagtg ctagatgcca aaaatgcgat tgaaaatggc
    87061 tgtcctaaag ttatcattgc atcaggaaac aagccaaata tcattgaatc tatttacaat
    87121 aatgattttg ttggcacaac aatccttaat tcataactat gaaattaagg cctaacaagt
    87181 tttgacacgc gagatgattc cagttcgatt atccattgcg ctaaaacatt tatttaccgt
    87241 tcatctcgtt aacaattttg aatacagtac gatacaatat gagatgtaaa aaactaataa
    87301 ccttttacaa atttgtttat caaaatattt taagttttgc aaagcttttt attgtgatta
    87361 ttttcacaaa atactataat gaggatagta aatagagagg agtccttaag ttgacgaaac
    87421 gacaaatggg tatattcatt tatgctggaa ttatcggtgg cttgttatct ggaattgtaa
    87481 aattaggttg ggaggtcatg tttccacctc gcacaccaga acgtaatgca acgaacccac
    87541 ctcaagagtt attgcaacaa ttaggattta gtagtgagtt tacgcatcaa acatatacat
    87601 tttcaaatat ggaattgcct tgggtaagct ttattgtcca ctttagtttt tctatcgtca
    87661 ttgcaattat ttactgcata ttagttaaaa aatacgctta cttagcaatg ggacaaggtg
    87721 ctgtttttgg tattgctatt tgggtattat tccaccttat cattatgcca atcatgcata
    87781 ctgtacctgc tgtgtgggat caaccattcc aagagcatct gtcagaattc tttggccata
    87841 tcgtctggat gatgacaatt gaattagtgc gacaacattt tgtctatcgc tataaattaa
    87901 attaatacac tgactaacat taacgtgagt tttaaatcat cgtttgagta tgatgattga
    87961 tgctcacgtt attttattaa ctgacatgat atgattccag ccaacttacg tgagcattaa
    88021 agtctcaaat gcgtcgtaac aaactattat tttcggtaat ttcaatattg ctcagtatat
    88081 ttttacctta tcacttactt taatctcgtc atgattttga atgatgccat cgtgtattca
    88141 cctttcattt ttccaataaa aaaacatcta acagtaaaca tttaggcagt atagtttaaa
    88201 atcactgcgc aatgatactg tcagacgtca tattaactac tcaataactg aaatacagac
    88261 acttttttat aaccccaggg tgcctgtcct aagaaacata cctgtaccat aaactgatca
    88321 aaaataaatt gtttgaactt cacttcacgt gattgataaa agtgtgattg tgtcatatca
    88381 taaatgtcca atcctttgat taaaccttca ccaatcaatt ttgtaaaact ttctttttgt
    88441 gtccatattt gataaaaatc atttaaacta catatttgat gtgcttcgtt tgtagagaaa
    88501 cacgtcacta acgtacgcca gtctaaacgt tgtgatatct tttcgatatc aataccaact
    88561 ggttctttat cgacaacaca cacgatataa ggataactat atgataagct cacatagatg
    88621 ggctgtccat cacgattgtg ttgaacaata tctgccttac ctcgtggcga aatgtgataa
    88681 tgccattcat gtggtaataa acctgtgtca tgttgaattc catattgcac taaaatatct
    88741 cccaatctgt gcatgagttt atcttgattg tatctatagt tgactgtacg cggtttttta
    88801 tatgaccaac gactttgtga tattaattct tcaatacttt tcaagttact ctgtaattgc
    88861 attacaaata ctgtcataac tttccctact tacttattga atattgtttt gatatattgt
    88921 gcccaatgat acagccaatt gttagttatc gttggccatt tttcactgat gtgattcatt
    88981 atttttaatg ttaatgttgt atctatcatt gctagttgtt gttcacggtc aacactagtt
    89041 aatccaatcg tttcgtacat gtcttgtttc tgtaaaattt cattaaatga ttcatcgctg
    89101 acgagttcaa tttctttgcg cttaacgcat tctaacaaag atttcaccgg cattttatta
    89161 ggtgatagca catggtaaat gatttgtggt gtgttgacct gtgctaatgc gacaatttgt
    89221 cttgcagtcg tatccacaaa agaaaaatct acaggcattt cagccatgct aaccccgata
    89281 caatccagtt gtaacaaatc attcattacc attgaaaaac ggttagtctt tatatttctc
    89341 atatgccatc ttccattgta aggattcgtc aaattaccaa cacgtacaat ccgaccatct
    89401 aagccattat ttacagcttc taatactttt aattcactat aaaatttgct ccgtgtatat
    89461 ggtgatgtta gtagttgccc tttatagaca tccgcttctg aaaatgtcac atcttctgtg
    89521 tctatatcaa aataagttcc cacacttatc gtagacacat atattaacct tgcatgatgt
    89581 tgttgtgcca aacgtatgac atcaacagta ccttgaacat ttactttttc aaattcatca
    89641 tcatcaccaa agtgatctgt acgagcacct gcatgaataa tcgtatccat gttttctggt
    89701 aaaacaacat catccataca ctcgaaatca ccaacaatga cttcaatgtt tgataacatt
    89761 atttcaaccg tctcttctga aaaataatca tttaaattcg tcatcaactt ataccatgct
    89821 atttcctcat tatcagcacg tatgaaacaa taaatgcgat gactgtatcc ttgtagtact
    89881 tcaatcagat aagcacctaa aaaacctgtc gcgccagtca atagtgtatt tcctagaggt
    89941 cgatgactta gactatcctc taaaataccc aagttataac gagacataac aatcttttgt
    90001 aattccgaaa gattatccgg taatgcaact aatgattgtt gattttggta catataatta
    90061 acaatctgtc gcacggtttt atattggtat aatgtctgca ttgaaatatg atggccaaat
    90121 cgttttaaat gcgagacaac taacatcgcc tctaatgagt taccaccaag ttcaaagaaa
    90181 tcatcgtcaa caccgacatc attttgtttc aatacctctc caaatacatc aacaaatgtc
    90241 tgctcaattt cattagaggg ttcgctatac actttattag actgttgtat aggtgatgga
    90301 tttggcaaac gcgtagtatc caccttgtca ttcgtggtta atggcataca atcgatatgc
    90361 gttatagtct taggaatcat atacttaggc agctgatcat ttaaatattg cttcaaatcc
    90421 tgttccactt gttgctctcc gacataataa gcattcaata tatcatgcgt atcaaagtga
    90481 cttactgtta caacacaatc agatatacca cgaatagcta atattgcatt ttcaatttca
    90541 tcaagttcaa tacggtaccc gttaactttc acttgtttat ctattcttcc taaaaattca
    90601 atttgaccat cagatgtata acgtgctaaa tcaccactat gatacaactt tcctttacca
    90661 aatggattat tttgccattt atcagccatt aattctggac gattaatata tcctatcgct
    90721 aaactatcac ctgcaataca caactcgcct ggcataccaa taccgcataa caaaccatct
    90781 gacataatat acacttggat gttagataag ggtttgccaa ttggaatcgt ctcaggtatc
    90841 aaatcaccac aatgatgtga ccaatacgat gtgatgactg ttgactcaga tggtccatag
    90901 gcattgaaat acgtgccaca atgcttctca atatatttaa caaaggatgc cgtactagtt
    90961 gccccgcctg taatcaactt ttcaatataa aagtcttcca taacactaca catctgtaac
    91021 ggaatcgacg caaccgtcac acgatgctta ttaatgagtt gttgtaactg ttctggatta
    91081 acacgttcct ctctatctgg aatcacaagc gtatgaccat ttaacaaaca acaataaatc
    91141 tccataactg atgcatcaaa aacaatattt gcatgttgca aaaatacttc attgtcgcct
    91201 aattgcaatt cagttgacca tgcatgcact aaattcaaca aatttcgttg tcgtatggca
    91261 acccctttag gcatcccggt cgtaccagat gtgtaaatag catacatctc attatctaac
    91321 atcgctgtgt tttcaagttg attgccatgt aaatcatcat attgttcatt ttcctttgat
    91381 tcaacaaagc ctttagcatt ttccaattga atgactggta atgtcgtttc aatttcaacg
    91441 ccgtaagaca tgactgcagt tactttagca tcctccaaaa ttgcaccttg tcgtttattc
    91501 ggaaaatcaa tatcgatagg tatataagat gcacctactt taactgtcgc caacatcgcc
    91561 gcaatcattt caaaactacg ttctgtaaac aaggcaaccc gttgaccatt gcccacacca
    91621 tttgatagga gcatgtgcgc aatggcatcc acatagttgc gtaatgtttc atacgtcatt
    91681 gtcaaatcat tcatgactag cgcaacatga ttaccttgtc gtgagacaac ttcattaaag
    91741 taacttatga tagatttatt tcccgggaca ttaagcattc gatcgttaac atgcgtattg
    91801 acccaattta gaagttcctc cgtgccgttt ggtatatcac aaatttgtag tgtatcttga
    91861 tgcttcaaaa tataatcaat cataatcata cattgattac ccatgtgacg aactgtttct
    91921 gagtgatata aatcggtatt atactcgata ttgattgtat agtcatcgcg atcttcttca
    91981 atgatgaaag ataaatcaaa tttcgccgtc actgatttgg gttgaatgtg tgttaattta
    92041 ctatgcccaa aatgagcatg attcgtttca ttgttttgta gtactaacat gacatcaaat
    92101 aatggattcc gtgaggcatc atgtgattga tctaagtcat ttactaaaca ttcgaatggg
    92161 tattcttgat gctcgtatgc ctccaaactc atttccttaa cctcttgtaa aaactgtgtc
    92221 cacattttat caggtgacgg ttgccctcta tataccaacg tattagcaaa catgcctagc
    92281 atttgctccg tgcctttatg catacgcgca ctcatcacac taccgacaac aacatcatct
    92341 tttcgagcat atctacttaa caacgtcatg accacactca taaagaacat aaaatcagta
    92401 atttgatgct tttctacata cttttgaagt agctgtctca tttgttgatt cattgtaaat
    92461 gacatcattg ctccatttgt cgttttaata tttggtctaa catagtctgt cggtaagctt
    92521 aaaataggta cttcatcttt gaattgagat aaccaatatt gtctatgttt cgtcatatca
    92581 cgatgcgaca tccactcact atagtcttta tattgcaatt taagtggtaa caataattta
    92641 tgttgataaa gtgcgttaag atcattcatt aattgtatat tactcatacc gtcattaatg
    92701 atatgatgcg tatctataaa gaggtatgca tgtaagggac ttctaatgta tctcactcta
    92761 atttgacttg gcttttccaa attaaaaggt gctacaaatt ggcgcatgat ttcttgttca
    92821 tccgtaaaat gcgtgttaac ttcttcaaag tcaactgcaa catctgccac aatacgttgt
    92881 cgaacctcat catctacaac aatatattgt gttcgtaaaa tctcatgtcg cgctatcaaa
    92941 cgctgcactg cttgtcgcaa ttgagctaca ttaagttctg atgataaccg ccataaaaaa
    93001 ggtacgttat acaccgtatc tttatggttt gatttccata ataaatacat acgcttttgt
    93061 gcagagctca gcacataatc atctttaact atagtttctg gaatcacttc atagttttgt
    93121 tcttgaacct tagcaatcgc ttgtgctagt tcaaatacag ttggcttttg taataaatca
    93181 ccaatttgta atcgtttccc agtagatgcc tctatccgat tcaccactaa cgttgctttt
    93241 aatgaatggc cacctaattc aaagaaatta tcatgaatac ctacttgatt cacatgtaaa
    93301 atatctgcaa aaatttggca tagcaagtgt tcggtatctg tactcggtgc tacataggca
    93361 tccgtatcga catagtccat gataggcaat gccttcttat ctaatttccc attaatagta
    93421 ataggaattt gctcaatatg catgaaatta actggtatca tgtactccgg taaggtcata
    93481 cgtaattgtg atttaatctt attatgtgat aatgtatgca tcgcttcata ataagcaacg
    93541 atatactgat cttgatcatg attttgaaca ataacaactg ctttattaat accttgtata
    93601 cgctcgagcg catgctcaac ctctgacaac tcaatcctaa accctcgaat cttaacttgt
    93661 ttgtcctttc gatataaata atctatgttg ccatcgggta acaaacgaac gatatcacca
    93721 cttctataca tcagctgatt tatatttgaa tctttgataa atttatctgc tgtcaattct
    93781 ggctgattta aataacctgc agctaaccca aagccacttg tacataattc tccaggaata
    93841 ccaacgccac accgacgctc gccttgcatg atataaacat gagtacccag aatcggttta
    93901 ccaataggaa tacgatttgg aactttgtta ggtatattat acgtcgttgt aaatgttgta
    93961 ttttcagttg gtccataacc attaataatt tgaggatgct tcggtttttg attaagcaaa
    94021 tccacccact tagcattcaa tacttctcca ccaattaata aatactttaa cggtaccaat
    94081 acttctattc gttcactagc aatctgatta aataatgagg aggttaacca catagtatta
    94141 acgtcatttt cattgattaa ttgttctacc gctattggat ttaataattg ttctttttta
    94201 gcaacaatca gctttccacc attgagcaat gcaccatata tttcaaatgt tgcagcatca
    94261 aaggctatag ttcctgataa caaaatcgtc gtctcttcat ttaatggtac ataatgattt
    94321 tgatggacca atcgaacaat acctcggtgc ggaattagtg tccctttagg gttaccagtt
    94381 gtccccgacg tgtaaataac ataagcatga tcttctaacg tgttacattt agaaagatta
    94441 tcaatatttt tccacgctat cttattcaaa tcaatgtgat taatattttg tttaccattt
    94501 tcatataaag cttggtacgt tattacaact ttaggcgtta catcttttaa aatgtactcc
    94561 tgacgatcac ttggatagtt cggatcaatt ggcacgtaag ccccaccagc tttcaacaca
    94621 cctatcatcg ctattatcat ctcaatactt ttttcagcta tgacagcgac acgatcatta
    94681 ggttcaacac catactggtt tctcaaacgg tgtgctaaat cattcgcgcg tgcattcaat
    94741 gtttgatatg ttataaacac tccgtcaaat tgcacagcga catgattcgg cgttgcttca
    94801 acttgttgct caaataaggt aacaactgtt tgcgcatcat ctatctcagg caaacttaaa
    94861 ttgatatcgt catataattg aatatcacgt tctgtcatca aattaagttc atctacagtt
    94921 gttcgtttat ttccattttc ttcagtaatt tgcaaataaa tatttcgaac taagtcactc
    94981 agcgtctcga ttgagagcaa atcatatgct gcactgttat atataatttt gaacccgtgt
    95041 tgatgtggaa aaatttcaat atccgctaaa gatgtatgtg catcatgtat ttgatgtaca
    95101 tcctcaataa catcattaca acaagacatc atatgatgat aacaatgaaa aatagtctct
    95161 agtgaaagcg aagacttcgc gcactgtaat tgcgacatat tttgcaacac acatttatta
    95221 aaatctgttg taaaacgttg acatacatct tttgcatcga ttgttaacgt taacggcaca
    95281 atatttccgt gtaaatcatt tggtaaatgt gatggtacat gtatacctaa tgtgacatca
    95341 tgttgttgac tcattatatg attagctaaa tacacactaa cagccaacga tgccatatct
    95401 attgatgtca tatcatcaat caaatacgtt tgatataaag cttgttcaaa tggatgctta
    95461 attggaaaat aactatcaat atggatgtca gagttattct ctaaccgaaa atagtttgag
    95521 tctaatgcta tatgcgatgc atcttgattg tctttatcat catttctatt tatatgtgca
    95581 tgctgtcgag tattgttaat aacagtattg ccacgatatg cattgcataa atcatcaaga
    95641 aaaatatcaa tttgactatc atcgaaaatg gacacatgaa aatctaatag tatatatgca
    95701 gcatcagcga actgaaacaa tttaactttg aataaaggtg aatcattaaa atggtaagta
    95761 cttaattctt gcttaaaaaa agcttctaaa tcatagtttg cggaagaaga tggaacttgt
    95821 tttatctcaa taaaaggcag aaattcatga agtatcattg ttaaattgtc atcggtagta
    95881 acatcaaaaa aatgtcttat agatgcatgt tgttgcacaa ttgtcgataa tgcatacatc
    95941 attttagtag cttcaacatt tttagcgagt ttaacccaat acgcattacg gtgtgtcgtt
    96001 gattctgtat tatttttgta tatacgaaaa tattcctgtt gaaatctcaa attacccata
    96061 atcataaaaa gtccttcttt catatcataa tactcattac ttactgaaat tgcatgatga
    96121 tatgataacc gacgaaatgt taattaactc gttatgtaat gattaatata aaacaccatt
    96181 cgcaacatat gagcgatata ttctacccta aaatacatct tgtatcatcg ttacaattgg
    96241 tatatttttc aatgtaaatt acatacatct tcgataaata gcacactaca actcgttaat
    96301 cactttctgt tgttcacatc tcattgcaaa ctcaatattg ttgttacaaa atatccatga
    96361 agcaagttta tattaaacaa acaactcgca taaaacaatt gttatcctta aattttaaca
    96421 aattcttaat aaatttatct ctattttaat tacgaccaaa ttaataggtt ttccatataa
    96481 aaagatgcat aaaataaata tttaaataaa ttcaatttgt attcacttgt ttttgtcccc
    96541 caaatacacc agcaacaagc atgctagcac caattgttaa aacgataaac atatacagtc
    96601 ccatttgtaa tgacgttaag aaaacaccca acacaatccc taacctagct agtgtttctg
    96661 aaaaatgaat acctaatgca ttaactgcac tatatgttcc tcttttagct ttaggaataa
    96721 ttttaaagcg ttgttctgaa actataggcg aataaataat ttcacctaca gtcgcaatta
    96781 tcataaaaac aactaataag ccaaactgat taagataggt tagaccacta tagccaacaa
    96841 tataaatcag caaaccagtg attaaagctt ttttaaaatc tattttcaat acaactttcg
    96901 agattgaata cgtgagtaaa atgacgacga ccgtattaat cattagcaag attgctaaca
    96961 tcttagcacc tgtaatatca tatgaaccta tacttattgt ttcaaactga tcctttagtc
    97021 taatagcaat atatgaggag attgaaaatt cacccatcat gatgatactg aaccccgaaa
    97081 tcaataacat ataattacgg tctttcaaaa ctaatttata actgcgaaat atattcatta
    97141 tttgtaattt ttgataacga cttgcatgcc tcttgtcatc actttgcttt acttgatttc
    97201 ggtcttgagg taaccaaata tataaaataa agagtacaat taaaaatata caagctgcta
    97261 ttaagaaaag tagtaacata ctgtagccat acatcaagcc acctaacaat gccccaatag
    97321 ctaccgataa gtttgtcatc caatagctaa tcttgtaaat ataatgttcc acgtcttcgg
    97381 taattgcatc cataattaat gtgtccataa ctggaaattg taatccccaa acgattgtaa
    97441 atatggcata tgcaacacaa aaaccaataa tttgccacaa ttgatgtgac ccaaatacgc
    97501 ccatgaacac aagcattatc accatcgtcg cttgataaat aagtactagc aactttttcg
    97561 gaaatatctc aataaggtaa ccagatataa tggacaatgg aaattttaga accactaaac
    97621 caacaagata tataccgaca attgattgac ttaacatatc tgttaaatat agtgctataa
    97681 acggtataaa tgctgtcgta ataattagct gtaaaaaatt gctaatcaat cgtactttca
    97741 aagtcgtact taagcgtttc attaataact acaccccttt ccacatatat acacactacc
    97801 atttatataa aatctattat tagattatac gttgaaacaa agaattttta gttatacaaa
    97861 gttatttaac aaacaaatgc atcgacttta caatgcatct tgcatataat gcctcaaaaa
    97921 aattagcact atatgactat atattattcc aaatgaatga aatttatcaa taattgcgta
    97981 agtgaacaca tatgtgcgta aatgttaaat cattgtccta acatagctgt gactatactt
    98041 agatgtaatc ataaaaaaac tactgccatc tttacaacag cagtagtgct tatatgattc
    98101 atttatataa aattgaaagc gttaatcgct agcccagcat ccttattcta tttagctgta
    98161 taacttttac tagcaatacg accacttgca acaataatat ctttatcttg gaaaggttca
    98221 tgattgaaga aacgctctaa aatatcttta actccatctt caatacgttt ttgtgcttct
    98281 aaagtcatac ctgaatagtg aaccgtcata gcatttctag gcattgttct ccatggatga
    98341 tcagcaggtg caggttgtgg ataccaaaca tcaccagcat atccttgtaa atgctcggat
    98401 gctaacgctt caactaacgc atcgcgattt acaattttac cacgtgcagt attcactaaa
    98461 taactgtgtt ttttcatacg acttaaaaca tctttatcaa ataagttatc agtttctggt
    98521 gttaatggtg catgaattgt aatcgcatca cttgttgaaa caagttcatc aaagcttaca
    98581 aatttagaca atttatggtc ttgttgattg attggatcat agtgttgtaa tgttacatta
    98641 aatggcgcta atctttcagc aacaagttgt ccaattcgac caaatccaaa aataccaatt
    98701 gttttgtgtt gtaattcatg cgcatgatta cctacttgag acaagttcca ttcaccttct
    98761 actgattgac gatgaccttc ttcatagttt ctaagaagta ttaataaatc cataaccgca
    98821 tgttctgcca cactaactgt attacttcct gtaacttcaa cgacaccaat attgtgttca
    98881 cttgctgccg ctaaatctac atggtcagat cctacacctg ctgtaattgc taatttcaag
    98941 ttcggtgctt tttcaatacg ttcacgagtc atatatgcag gataaaatgg cgcactaata
    99001 atcacatcca tatctggtaa atgtttatct aagtcttcac cattatctgc taatataatg
    99061 aactcatgtc ctctttcctc taaaaatgtt tttaatccta atgctttttt agtattaagt
    99121 aattgatttt cttgaccttc tactgcttct ggaaataatg ctacgatttt catatccatc
    99181 tctcctaaat gataaatttt gacttcctta aagtacgtgt tgcatgttgc ttgttttaaa
    99241 aatatgacaa aaaaacggca ccgtttgaca taccaccccc ttgaattatt gttaaattca
    99301 aagcgaagtt agcaaacagc accgtgtacc ttacaccagc aacacttatt ctattgactt
    99361 atcgaaaaca aatattgaaa tgacttcatc atcattcaaa tcaatatctg taaataattt
    99421 tacaaactta gcgcctacaa gactttccat ctcatttaca tctatttctt tatacatctg
    99481 tttaatcttc tctgtgcgtg tataatggag catcgattca ttgcgtttgt cgtttaggta
    99541 aaaactctca actttactca aaacacctgt catatgtgca atcgcccaat tatctttaaa
    99601 cgacactcga atactattgg gtcctttacc cacgtattct ttacgataag atcttactag
    99661 attcgagaat tgttgttctt tagtatgcgt atctatagta ttcaccttct ttaaaataag
    99721 aagttcatta attgttcaca ttttcattat acaccttatt aacacttttt caatgataac
    99781 gattattatt attac
//
LOCUS       DALAGF010000002        62416 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.002, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000002 DALAGF010000000
VERSION     DALAGF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 62416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62416
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.002"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            89..436
                     /gene="sarR"
                     /locus_tag="O2692_000077"
     CDS             89..436
                     /gene="sarR"
                     /locus_tag="O2692_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HCZ4297729.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
     gene            complement(625..1461)
                     /locus_tag="O2692_000078"
     CDS             complement(625..1461)
                     /locus_tag="O2692_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HCZ4297730.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(1461..2075)
                     /gene="ureG"
                     /locus_tag="O2692_000079"
     CDS             complement(1461..2075)
                     /gene="ureG"
                     /locus_tag="O2692_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HCZ4297731.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            complement(2088..2777)
                     /locus_tag="O2692_000080"
     CDS             complement(2088..2777)
                     /locus_tag="O2692_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HCZ4297732.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEGTRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            complement(2770..3222)
                     /gene="ureE"
                     /locus_tag="O2692_000081"
     CDS             complement(2770..3222)
                     /gene="ureE"
                     /locus_tag="O2692_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HCZ4297733.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            complement(3235..4950)
                     /gene="ureC"
                     /locus_tag="O2692_000082"
     CDS             complement(3235..4950)
                     /gene="ureC"
                     /locus_tag="O2692_000082"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HCZ4297734.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            complement(4947..5357)
                     /locus_tag="O2692_000083"
     CDS             complement(4947..5357)
                     /locus_tag="O2692_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HCZ4297735.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS"
     gene            complement(5371..5673)
                     /locus_tag="O2692_000084"
     CDS             complement(5371..5673)
                     /locus_tag="O2692_000084"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HCZ4297736.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            5933..6838
                     /gene="yut"
                     /locus_tag="O2692_000085"
     CDS             5933..6838
                     /gene="yut"
                     /locus_tag="O2692_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HCZ4297737.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYTFARFINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKATLLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            7055..8209
                     /locus_tag="O2692_000086"
     CDS             7055..8209
                     /locus_tag="O2692_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCZ4297738.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            8423..9331
                     /locus_tag="O2692_000087"
     CDS             8423..9331
                     /locus_tag="O2692_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ4297739.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            9730..10671
                     /locus_tag="O2692_000088"
     CDS             9730..10671
                     /locus_tag="O2692_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCZ4297740.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            10792..11346
                     /locus_tag="O2692_000089"
     CDS             10792..11346
                     /locus_tag="O2692_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCZ4297741.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGIK"
     gene            11418..12188
                     /locus_tag="O2692_000090"
     CDS             11418..12188
                     /locus_tag="O2692_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4297742.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNH
                     KPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(12360..13157)
                     /gene="fdhD"
                     /locus_tag="O2692_000091"
     CDS             complement(12360..13157)
                     /gene="fdhD"
                     /locus_tag="O2692_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCZ4297743.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPMQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            13447..14229
                     /gene="modA"
                     /locus_tag="O2692_000092"
     CDS             13447..14229
                     /gene="modA"
                     /locus_tag="O2692_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCZ4297744.1"
                     /translation="MKLKRLFAVVIAMLLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            14243..14914
                     /gene="modB"
                     /locus_tag="O2692_000093"
     CDS             14243..14914
                     /gene="modB"
                     /locus_tag="O2692_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCZ4297745.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKLLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            14915..15520
                     /locus_tag="O2692_000094"
     CDS             14915..15520
                     /locus_tag="O2692_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ4297746.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            15690..16694
                     /locus_tag="O2692_000095"
     CDS             15690..16694
                     /locus_tag="O2692_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HCZ4297747.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKYRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            16725..17231
                     /locus_tag="O2692_000096"
     CDS             16725..17231
                     /locus_tag="O2692_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HCZ4297748.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     DGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(17233..17727)
                     /gene="moaC"
                     /locus_tag="O2692_000097"
     CDS             complement(17233..17727)
                     /gene="moaC"
                     /locus_tag="O2692_000097"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCZ4297749.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTLIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSNQ"
     gene            17790..19049
                     /locus_tag="O2692_000098"
     CDS             17790..19049
                     /locus_tag="O2692_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCZ4297750.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETKTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFVDGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            19046..19531
                     /gene="mobB"
                     /locus_tag="O2692_000099"
     CDS             19046..19531
                     /gene="mobB"
                     /locus_tag="O2692_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCZ4297751.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            19545..19991
                     /locus_tag="O2692_000100"
     CDS             19545..19991
                     /locus_tag="O2692_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HCZ4297752.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            19997..20230
                     /gene="moaD"
                     /locus_tag="O2692_000101"
     CDS             19997..20230
                     /gene="moaD"
                     /locus_tag="O2692_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HCZ4297753.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            20237..20836
                     /gene="mobA"
                     /locus_tag="O2692_000102"
     CDS             20237..20836
                     /gene="mobA"
                     /locus_tag="O2692_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCZ4297754.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            20856..21878
                     /gene="moaA"
                     /locus_tag="O2692_000103"
     CDS             20856..21878
                     /gene="moaA"
                     /locus_tag="O2692_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCZ4297755.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(22273..22443)
                     /locus_tag="O2692_000104"
     CDS             complement(22273..22443)
                     /locus_tag="O2692_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297756.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNGMECDINVKEPLKIYYV
                     VILKSGIEMIFS"
     gene            22569..22919
                     /gene="sarV"
                     /locus_tag="O2692_000105"
     CDS             22569..22919
                     /gene="sarV"
                     /locus_tag="O2692_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HCZ4297757.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(23021..24232)
                     /locus_tag="O2692_000106"
     CDS             complement(23021..24232)
                     /locus_tag="O2692_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4297758.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFLVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(24225..24665)
                     /locus_tag="O2692_000107"
     CDS             complement(24225..24665)
                     /locus_tag="O2692_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HCZ4297759.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLREAHND"
     gene            24827..25591
                     /locus_tag="O2692_000108"
     CDS             24827..25591
                     /locus_tag="O2692_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4297760.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(25583..25834)
                     /locus_tag="O2692_000109"
     CDS             complement(25583..25834)
                     /locus_tag="O2692_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297761.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLVIIHMGILAHSTYVYLY"
     gene            26223..27488
                     /gene="femX"
                     /locus_tag="O2692_000110"
                     /gene_synonym="fmhB"
     CDS             26223..27488
                     /gene="femX"
                     /locus_tag="O2692_000110"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HCZ4297762.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            27605..30772
                     /locus_tag="O2692_000111"
     CDS             27605..30772
                     /locus_tag="O2692_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCZ4297763.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVATIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(30959..31276)
                     /gene="mspA"
                     /locus_tag="O2692_000112"
     CDS             complement(30959..31276)
                     /gene="mspA"
                     /locus_tag="O2692_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HCZ4297764.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(31521..31685)
                     /locus_tag="O2692_000113"
     CDS             complement(31521..31685)
                     /locus_tag="O2692_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297765.1"
                     /translation="MSLENQLAELKYDYVRLQGDIEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            31818..32726
                     /locus_tag="O2692_000114"
     CDS             31818..32726
                     /locus_tag="O2692_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCZ4297766.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFVMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVYVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     TFIGMTTNLTIIISIIILYVYQAVFV"
     gene            32897..33028
                     /locus_tag="O2692_000115"
     CDS             32897..33028
                     /locus_tag="O2692_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297767.1"
                     /translation="MYGAYLIYEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(33145..34008)
                     /locus_tag="O2692_000116"
     CDS             complement(33145..34008)
                     /locus_tag="O2692_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HCZ4297768.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(34189..35076)
                     /locus_tag="O2692_000117"
     CDS             complement(34189..35076)
                     /locus_tag="O2692_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4297769.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            35142..35237
                     /locus_tag="O2692_000118"
     CDS             35142..35237
                     /locus_tag="O2692_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297770.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            35263..37398
                     /locus_tag="O2692_000119"
     CDS             35263..37398
                     /locus_tag="O2692_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCZ4297771.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            37511..38845
                     /locus_tag="O2692_000120"
     CDS             37511..38845
                     /locus_tag="O2692_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCZ4297772.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(39037..39426)
                     /locus_tag="O2692_000121"
     CDS             complement(39037..39426)
                     /locus_tag="O2692_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297773.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            39783..40091
                     /gene="rpsJ"
                     /locus_tag="O2692_000122"
     CDS             39783..40091
                     /gene="rpsJ"
                     /locus_tag="O2692_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCZ4297774.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            40119..40781
                     /gene="rplC"
                     /locus_tag="O2692_000123"
     CDS             40119..40781
                     /gene="rplC"
                     /locus_tag="O2692_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCZ4297775.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            40808..41431
                     /gene="rplD"
                     /locus_tag="O2692_000124"
     CDS             40808..41431
                     /gene="rplD"
                     /locus_tag="O2692_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCZ4297776.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            41431..41706
                     /gene="rplW"
                     /locus_tag="O2692_000125"
     CDS             41431..41706
                     /gene="rplW"
                     /locus_tag="O2692_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCZ4297777.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            41739..42572
                     /gene="rplB"
                     /locus_tag="O2692_000126"
     CDS             41739..42572
                     /gene="rplB"
                     /locus_tag="O2692_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCZ4297778.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            42639..42917
                     /gene="rpsS"
                     /locus_tag="O2692_000127"
     CDS             42639..42917
                     /gene="rpsS"
                     /locus_tag="O2692_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCZ4297779.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            42946..43299
                     /gene="rplV"
                     /locus_tag="O2692_000128"
     CDS             42946..43299
                     /gene="rplV"
                     /locus_tag="O2692_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCZ4297780.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            43323..43976
                     /gene="rpsC"
                     /locus_tag="O2692_000129"
     CDS             43323..43976
                     /gene="rpsC"
                     /locus_tag="O2692_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCZ4297781.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            43979..44413
                     /gene="rplP"
                     /locus_tag="O2692_000130"
     CDS             43979..44413
                     /gene="rplP"
                     /locus_tag="O2692_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCZ4297782.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            44403..44612
                     /gene="rpmC"
                     /locus_tag="O2692_000131"
     CDS             44403..44612
                     /gene="rpmC"
                     /locus_tag="O2692_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCZ4297783.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            44636..44899
                     /gene="rpsQ"
                     /locus_tag="O2692_000132"
     CDS             44636..44899
                     /gene="rpsQ"
                     /locus_tag="O2692_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCZ4297784.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            44931..45299
                     /gene="rplN"
                     /locus_tag="O2692_000133"
     CDS             44931..45299
                     /gene="rplN"
                     /locus_tag="O2692_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCZ4297785.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            45335..45652
                     /gene="rplX"
                     /locus_tag="O2692_000134"
     CDS             45335..45652
                     /gene="rplX"
                     /locus_tag="O2692_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCZ4297786.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            45679..46218
                     /gene="rplE"
                     /locus_tag="O2692_000135"
     CDS             45679..46218
                     /gene="rplE"
                     /locus_tag="O2692_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCZ4297787.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            46241..46426
                     /locus_tag="O2692_000136"
     CDS             46241..46426
                     /locus_tag="O2692_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCZ4297788.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            46458..46856
                     /gene="rpsH"
                     /locus_tag="O2692_000137"
     CDS             46458..46856
                     /gene="rpsH"
                     /locus_tag="O2692_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCZ4297789.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            46881..47417
                     /gene="rplF"
                     /locus_tag="O2692_000138"
     CDS             46881..47417
                     /gene="rplF"
                     /locus_tag="O2692_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCZ4297790.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            47448..47807
                     /gene="rplR"
                     /locus_tag="O2692_000139"
     CDS             47448..47807
                     /gene="rplR"
                     /locus_tag="O2692_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCZ4297791.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            47828..48328
                     /gene="rpsE"
                     /locus_tag="O2692_000140"
     CDS             47828..48328
                     /gene="rpsE"
                     /locus_tag="O2692_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCZ4297792.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            48345..48524
                     /gene="rpmD"
                     /locus_tag="O2692_000141"
     CDS             48345..48524
                     /gene="rpmD"
                     /locus_tag="O2692_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCZ4297793.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            48541..48981
                     /gene="rplO"
                     /locus_tag="O2692_000142"
     CDS             48541..48981
                     /gene="rplO"
                     /locus_tag="O2692_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCZ4297794.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            48981..50273
                     /gene="secY"
                     /locus_tag="O2692_000143"
     CDS             48981..50273
                     /gene="secY"
                     /locus_tag="O2692_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCZ4297795.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            50290..50937
                     /locus_tag="O2692_000144"
     CDS             50290..50937
                     /locus_tag="O2692_000144"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCZ4297796.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            51130..51348
                     /gene="infA"
                     /locus_tag="O2692_000145"
     CDS             51130..51348
                     /gene="infA"
                     /locus_tag="O2692_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCZ4297797.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            51380..51493
                     /gene="rpmJ"
                     /locus_tag="O2692_000146"
     CDS             51380..51493
                     /gene="rpmJ"
                     /locus_tag="O2692_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCZ4297798.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            51516..51881
                     /gene="rpsM"
                     /locus_tag="O2692_000147"
     CDS             51516..51881
                     /gene="rpsM"
                     /locus_tag="O2692_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCZ4297799.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            51905..52294
                     /gene="rpsK"
                     /locus_tag="O2692_000148"
     CDS             51905..52294
                     /gene="rpsK"
                     /locus_tag="O2692_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCZ4297800.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            52369..53313
                     /locus_tag="O2692_000149"
     CDS             52369..53313
                     /locus_tag="O2692_000149"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCZ4297801.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVTPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            53330..53698
                     /gene="rplQ"
                     /locus_tag="O2692_000150"
     CDS             53330..53698
                     /gene="rplQ"
                     /locus_tag="O2692_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCZ4297802.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            54236..55045
                     /locus_tag="O2692_000151"
     CDS             54236..55045
                     /locus_tag="O2692_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCZ4297803.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPYDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDNAEELTRIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            55042..55902
                     /locus_tag="O2692_000152"
     CDS             55042..55902
                     /locus_tag="O2692_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCZ4297804.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            55892..56698
                     /locus_tag="O2692_000153"
     CDS             55892..56698
                     /locus_tag="O2692_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCZ4297805.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTISLTMIIPIAIILFVLKYSGV"
     gene            56703..57506
                     /gene="truA"
                     /locus_tag="O2692_000154"
     CDS             56703..57506
                     /gene="truA"
                     /locus_tag="O2692_000154"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCZ4297806.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLGKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            57746..58183
                     /gene="rplM"
                     /locus_tag="O2692_000155"
     CDS             57746..58183
                     /gene="rplM"
                     /locus_tag="O2692_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCZ4297807.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            58197..58595
                     /gene="rpsI"
                     /locus_tag="O2692_000156"
     CDS             58197..58595
                     /gene="rpsI"
                     /locus_tag="O2692_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCZ4297808.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            59140..60237
                     /locus_tag="O2692_000157"
     CDS             59140..60237
                     /locus_tag="O2692_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCZ4297809.1"
                     /translation="MRGDQRTQGEKSRKEGGKIFGSGSRAPIAISILVKDGSYNHDIY
                     YNDIGEYLTREQKLDTLMKHQSIVNLKSLNVLPDKNNDWINQRDINYENYLPMYDSKD
                     IENSIYLDQFNGVNSARDNWVTNFSNEKALVNAKLLVDNYNSEIDRLIDILDSRERIN
                     LVNKDETFISWTRGLTQKFSKGKNISINPERIVKFMHRPFTKKWIVYDKNIMEMPSRY
                     YNIMENTGQVIYIQGQGMNKEFSAMITDILPNFQFIGNGKGFATYKGKDSLRLVDNIS
                     NSFKKKINLNSEEIVYYIYAILHHKYYVNKYSSDLSKGFPRIPILKDVYGFVEIGREL
                     VELHLNYEKQLNWDGVEIIYNNMNPNYKVEK"
     gene            60234..60527
                     /locus_tag="O2692_000158"
     CDS             60234..60527
                     /locus_tag="O2692_000158"
                     /inference="COORDINATES: protein motif:HMM:NF037202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297810.1"
                     /translation="MKHPKKGVLDTVIYNTDITVTNIPNKVYNYIVNGKPAIEWIIDQ
                     YNVSIDKKSGILDDPNEFSEDPNYILNLLLSVITVSMKTLGLIDKLPDFEVLD"
     gene            60588..61331
                     /locus_tag="O2692_000159"
     CDS             60588..61331
                     /locus_tag="O2692_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297811.1"
                     /translation="MDLLQIEKKGNLTKTVQLMHDEKGSASGESKVNMEFGKQSAVKQ
                     IFDSLLGMSASASASAGINGNITGERVAKTILENTLIYDFLDTVEFRKKKTIIDISEG
                     FKLNIQQDTMTYFATITPIASMFEGSKHLDEEELSFDVSKMNEAIRSIKGYFELIGTR
                     GNENRVFRFNIESFKNNYRIQDLKFMNLTLYSVLVGEISKENLNYDKEFDLNQGAKPS
                     GIGKFKGIRSEATKEDKYQVYDVILAGVK"
     gene            61332..>62416
                     /locus_tag="O2692_000160"
     CDS             61332..>62416
                     /locus_tag="O2692_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCZ4297812.1"
                     /translation="MEIHVYTGPLCGFNEYINSEVKEYHRFMELIREYNIIVRASDTF
                     GTHDVDGIFDTEIENLVIFSNDFASVTTHVITNFINIVTLGRNIKKIYIQNPPKRVFQ
                     SLNSFHDSEIIEINYEYTTLKKDDVINFYKEQMAGSKIIGQTDAKKQMSIDLYKHTSL
                     ENNKPTVMLYYGPSGVGKTELAKEIAKKYKGNITRIQFSMMQNDESFNYIFGDEHAKV
                     SLSKDLVDRETNVVLIDEFDKVLPIYYNVFYQMFDEGIIEDANYKVDVSNCIFILTSN
                     FNSIEEAIVNIGEPVFSRIDSCIKFLYLTQEEKQKVIENKLNEILSSLNEKEKRIVTA
                     YNLLEKYKETEIDIDNIRYLKKIITND"
BASE COUNT        22377 a         9272 c        11679 g        19088 t
ORIGIN      
        1 ttataacata gttggataga gtttcgattt aataaattac atgtgaacct tgctacaaca
       61 agatgtgcat cagaggagtg gtttaataat gagtaaaatt aatgacatta atgatttagt
      121 caacgcaaca tttcaagtta agaagttttt cagagataca aaaaagaagt tcaatttgaa
      181 ctatgaagaa atttatattt taaatcatat tttaagaagt gagtctaacg aaatctcatc
      241 taaagagatt gctaagtgct cagagttcaa accttactat ttaactaaag ctttacaaaa
      301 gctaaaagat ttaaaattgt tatcaaagaa aagaagttta caagacgaaa gaacagttat
      361 tgtttatgtt acagatacac aaaaagcaaa tattcaaaaa ctgatttcag aattagaaga
      421 atacattaaa aattaaatca aggttaattg cgtttaataa cattgaacga taacaattta
      481 ttaaaacgaa gttatttatt cagcattggg acataaaatt aacttaaaat ttaaatattg
      541 aagatgcttt aattaaagtt aaagactagc cataccttat ttcagcttat taagcttgaa
      601 acaaggtaca ctagtctttt tattttaata ttttcttaga aaatcaagtt tacgatcata
      661 aatattttct gcgatatagc tttggatggt tccaagtatt ttctctataa tttgtgtgcg
      721 ataagcaaaa attctaactg caaaaccatg tgtaggcaat tgagaaatag caacacgaca
      781 atcgaatgta ttgctataag aactaatggt ttcataaact gaatcgatta gtttttggtt
      841 aacatcttcg tgtatgaagt aagcagagcc ataatgagag taatgttcca tataacctat
      901 ttcattgatc gattgtttat taggattcaa taataaatta tcatatgtga ctaactcatc
      961 ttcaatataa atttcattta ttaaatgcat atattgatac ttgaaggctt cgccagtttt
     1021 tgaataacca ggagttaaaa tatcagtata aaataatgaa ctagaattat tgagattgaa
     1081 cgtattgtgt tgataaaatt tagcattttc atatgcgatg attggatcag cgacatattc
     1141 taaatatgcg ttatctttca aattaaaagt ttgatactgc tcaacatgat tggaaggtgt
     1201 cttgtatatt tttgttgcac cttgagatgt caatgtcact ttagcgttat cttcgacatt
     1261 cacattcata cggtaacgat ctccatctaa atagccacca cctacattta ctatataaaa
     1321 tgtaggaatg gtagattgat ttagataaac tggacgtatc actttaagtg ctttttcaaa
     1381 gaaaatatct cttgatactg atcgattgcc atcgaaaaac actgttaaat caagttgccc
     1441 agtccattgt tgttcatcca ttatgataat cctttgagta aagtgtcgcg ttcaatccaa
     1501 tcgataactt catccaaacc ttcgtcggtt tttaagttag taaaagtaaa tggacgttta
     1561 ccacgaaata ctttagtatc ttcagccatt tgttctaatg atgcacctac atagggagct
     1621 aaatcagttt tgttaattac aaagaaatct gacttaatca taccttgacc acctttacgt
     1681 ggaatctttt caccttgagc aacatcgata atatatattg aaaagtcaac aagttctgga
     1741 ctaaatgttg ctgctaagtt atcgccacca gattctatga aaattagttc tatatcgtca
     1801 tgacgttcta ataattcgtc tattgctgca aagttcatag atgcatcttc acgaatcgca
     1861 gtatgaggac atccaccagt ttcaacacca atgatacgac tttcaggtag aactcctgaa
     1921 tttactaata tcttttcgtc ttcttttgta tatatatcat ttgtaataac gccgatactc
     1981 atttcttttg aaagacgttt tacaactttt tcaattaatt gtgttttacc tgcacctaca
     2041 ggaccaccaa taccaatttt aatcggattt gccacaatta taacctccta tgatatgaaa
     2101 attctaacat tgacgttctc atgcgccatt tgatttagtt ctaaaccagg cgctgtcatg
     2161 ccaaaatctg cttcttttaa ttcgaaaatc tgctttcttg ttccttctat ataaggaatc
     2221 atgtgagtaa ctatcttttg accagcagtt tgtccaagtg gaatagcacg aacagcattt
     2281 tgagttaaac ttgaaacatt ttgatataaa tagtaatcaa taatcgtttc aatatctaca
     2341 cctaaatgat ggcctagcat agtaaaacaa atagctggat ttaactttgc tttcttatct
     2401 tgcatttgtt gatgatacca agcaatccat gggctattat aaagttctaa agccaattta
     2461 accatgcgag tccccatttg ttttgcacca acacgtgttt ctttaggtaa gttttgaaca
     2521 aacatcagtt tatctatgtg taatactttt tgtgtatcat cattttccaa tgcatcataa
     2581 actaaacgca tggctaaacc atcagaatag gtaagttgct cttgtaaaaa catttttaac
     2641 caagcaataa aagtatgatc gtcatgaatt atatttcgtt gaatatatgt ttcaagacca
     2701 aatgaatgac tgaaagcacc tgttggaaac tgtgaatcac agaactgaaa taatcttaag
     2761 tgtgtatgat caatcatgag aatgccctat atgtctgaaa gccttattaa ctttacggtc
     2821 ttctcgaaca tatgggatgc ctaaactttt taataaatct tcaactaaat aatcatattg
     2881 tactagcatt tcagtctctg taaattgtgc tggcaaatga cgatttccta attgatgggc
     2941 tatatctccc atttcttgca atgttcttgg ttgaatcact aaaagatctt ctgaattaac
     3001 atccacaata atcatattat ggtcatctgc gtataaaata tctccatatt gtaagtcaat
     3061 aggttgtttt aaacgaatgc ctatttcagt gccatggtct gtaacgactc tttgaatacg
     3121 tttaacaaga tctgaatttt caaggtatac tttttcgacg tgcttttgtt tttctgaatt
     3181 tgacaaattg gcaatattgc cttggatttc ttcaacaatc attctatgtt cctcctagaa
     3241 taagaagtat ctttgagtta atggtaactc agttgctgca ttacttgtaa ttttttctcc
     3301 atctacatat acttcatatg tttgtggatc aacgtctaat ttaggtgttg cactgttatt
     3361 tttcatatct gctttagata attgtctaat atttttaact ggacgcacca tgcgttttaa
     3421 atttaatgca cgattgatac cattttcata agcagtttta gacacgaatg tcattgacgt
     3481 acttgtaagg tttccgccgt attgaccata cattttacgg tacttcatcg gttcagatgt
     3541 aggtatagaa ccatttgcat cgccatttac ggcagagtta attaatccgc cctttacaac
     3601 taattcaggt ttaaccccaa agaaaattgg gtcccataag acaatgtcag ctagtttgcc
     3661 cggctcgata gatcctacat attcagaaat accatgtgta attgctgggt taattgtata
     3721 tttagcgata taacgtttga tgcgattatt atcattatgt tcaaaatcac catctaaagg
     3781 accacgttgt tctttcatgc gatgtgctac ttgccatgtt cgtgtaatta cttcacctac
     3841 acggcccatt gcttgtgaat cggaactaat catactgaat acacccatat cttgcagaac
     3901 atcttctgct gcaatcgttt ctttacgaat acgtgaatct gcgaatgcga tatcttcagg
     3961 aatagccgca tttaaatggt gagtaatcat taccatatct aaatgttcat ctacagtatt
     4021 atgtgtataa ggcaaagttg gatttgtaga tgaaggtaaa atatttgaaa atgcagcgga
     4081 tttaattaaa tcaggcgcat gaccgccacc agcaccttca gtatggtaca tatgaagtac
     4141 acggtcttta acagcagcca ttgtgtcttc cataaatcct gcttcattta aagtatctgc
     4201 atgtaatgca atttgaacat caaattcatc agcaacatct aatgcatgac tcaaagcaga
     4261 tggtgttgca ccccagtctt catgtacttt taatccaatt gctccggcat tgatttgttc
     4321 aatgagtgca gttggatttg ttgcttgtcc tttacctgta aaaccgacat taatcggtaa
     4381 accttcggca gcttctaaca ttctatgaat atgccatgga cctggagtta cagttgttgc
     4441 tttagaacct tctgaagcac cagtaccacc accaatatga gtcgtaatac cactttctaa
     4501 tgcgacctct gcttgttcag gattaataaa atgaacatga gtatcaatac caccagcagt
     4561 gacgatttta ccttcagcgg caatgatatc tgttgttgaa cctataataa tgtcgacatt
     4621 atccattata tctgggttgc cggcattacc tatggcgaaa atataaccat ttttaatgcc
     4681 tatatcagct ttaaccactt tatcgtaatc gataataacg gcattagaaa tgacaaggtc
     4741 tgcaacgttc acgtcatcac gtgttacacg aggattttgc gccataccgt ctctaataga
     4801 tttaccacca ccaaaagtag cttcttcacc ataaaccgca tagtcttttt ctatttgagc
     4861 aaatagattc gtatcaccta aacgaatgga atctccaaca gttggaccgt ataagctcgt
     4921 atattgattt tgcgtcattt taaagctcat gatctttttc ctcctttttt attcacgttt
     4981 tcagcaccgt tatctccgaa tacacctgca tattcatcat tttcatcagt tgggcgatag
     5041 acacgtgact catcgatagg accattgacc ataccacgaa aaccaaaaat tttacgtttg
     5101 ccagcatatt caactaattg aacttctttt ttatccccag gttcaaatcg aacagctgct
     5161 ccagctggaa tatctaaatg ttttccatat gccatttcac gttcgaaatc taatgctgca
     5221 tttgcttcat aaaaatgaaa atgtgagccc acttgaattg gtcgatctcc tgtattttca
     5281 acttcgataa ctgtttcagg atgatggtta ttaatttcaa cctctgtact ttttgtaata
     5341 atttctcctg gtatcatttg actgcctcct ttaaacaata gggtgatgta ctgtgattaa
     5401 cttagtacca tcggggaacg tagcctcgat ttcgatatct gtaatcatgt gttcgacacc
     5461 atccatgaca tcttctttgt ttagaatttg tctaccataa ctcattaact ctgcaacggt
     5521 cttaccatcg cgtgcacctt ctaataattc atcgctgatt aaagctaatg cctcaggatg
     5581 atttagtttc aaaccacgtg ctttacgacg acgtgcaact tccgccgcca ctacaatcat
     5641 taatttgtct tgctctcgtt gtgtaaaatg caaattaaaa cccccaattt catattagat
     5701 acaatttaca aaatttatat taatcctaat tgttgtgata aacaagtaat atacaaagtt
     5761 caatgtgtaa ttagaaaatt atatttttag catatccgat attgaagcaa acaatctaat
     5821 cgaaaacaaa tagtggaata tatttatgta aaaaccaaaa tagtttttaa tataactttt
     5881 catagaatag tagtatatta atgagtaatg attcaaagga aaggtgaaag atttgaagat
     5941 aatagatgtg cttttgaaaa atatatctca ggttgtgtta attagtaata aatggacagg
     6001 attatttatc ttaataggat tatttgtagc cgattggaca attggattag cggctattgt
     6061 aggtagcatc atcgcctata cttttgcgcg ttttataaat tatagtgagg cagagattaa
     6121 tgatgggtta gctggattta atccagtgct aactgccatt gcgttaacaa tctttttaga
     6181 taagtcagga ttagatattg ttataacaat gatagcaact ttattaacgt taccagttgc
     6241 tgctgcagtg agagaagttt taagaccata taaagttccg atgctgacga tgccttttgt
     6301 cattgtgact tggtttacaa ttttactttc aggacaggtt aaatttgtag atacatcgtt
     6361 aaagttaatg cctcaaaaca ttgaaacggt taattttagc aacaatgata gaatacattt
     6421 cattcagtca ttatttgaag gattcagtca agtatttatc gaagcgagtg taattggtgg
     6481 cgtatgtatt ttaatcggca tattgatagc atcaagaaaa gcaacactct tagctgttat
     6541 agctagtttg ttaagcttta tcattgtagc tctattaggt ggtaattatg atgatattaa
     6601 tcagggatta ttcggttata actttgtatt aatggcaatc gcactaggat atacatttaa
     6661 aacagcgatt aacccttata tttcgacttt tttaggtgtg ttattaacag tagtggtgca
     6721 actaggtaca acaacattgc ttgaaccgtt tggcttacct gcattaacat tgccatttat
     6781 tatcgtgaca tggattttat tatttgctgg tattaaacat gacaaagtag atgcttgata
     6841 gttaaatcaa acctaatatt gtttgaatat caccttaaac tatacagcga attgtatagt
     6901 ttaaggtgta tttttatgga taaaattaag tgcatactta atcgaattat cagttttctt
     6961 ttaattgttc acagcacgtt cctttttatt tttaaagtcg agtgttaaac taggaataaa
     7021 taatttatat tgtgtgttgt gtggggtgac taatatgaat gatatggata attccttttt
     7081 aataacaacg gaaattcaaa gaaaatggat tgaaaaattc aaagtaatta gagatacatt
     7141 taaggctaaa gctgaatata atgatcaaca tagccaattt ccatataaaa atattgaatg
     7201 gttaattaaa gaaggttatg gaaaattaac gttaccaaaa gcatatggtg gtgaaggtgc
     7261 gaccatagaa gacatggtta ttttgcaatc atttttaggc gaacttgatg gtgccacagc
     7321 attatctatt ggttggcatg tgagtgtcgt aggacaaatt tatgaacaga aattatggtc
     7381 tcaagatatg ttggagcaat ttgctgttga aattaataat ggtgcattag ttaatagagc
     7441 agttagtgaa gctgaaatgg gtagtccaac aagaggggga agaccaagta cacatgctgt
     7501 taaagctgat gatgggtata ttttaaatgg tgtgaagaca tatacatcaa tgagtaaagc
     7561 actaacacat attattgttg ctgcttatat agaagaatta gaaagtgttg gttttttctt
     7621 agtagacagg aatttgcctg gtgttgaaat tgccgataat tgggacgtat taggtatgcg
     7681 agcgacagaa agtcatgatt taatattaaa tgatgtaaaa gtaccattaa aacatttagt
     7741 ggaaacagag aaaagtaaag cgccaaatgg ttggatattg catataccaa gctgttattt
     7801 aggtatagca caagctgcta gaaattatgc tgtagacttt gcaatccaac atagccctaa
     7861 tagtattgaa ggaacgattg caacattgcc aactgtgcaa caaaatttag ggaaaatgga
     7921 aacgctattg ttatgtgcga gacaattttt atggagtaca gcaaaagggt atcaacaata
     7981 taaagatgac agtcagataa gaaatccaac aagtgcgagt aaagtgatgg taatgaatca
     8041 agggcttgaa gtgattgatt tagcgatgag aatagttggt gctaaaagct tagaaatgaa
     8101 tcgaccatta caacgttact atcgtgacat gcgtgcagga ttacacaatc cacctatgga
     8161 agatgcagca tatacaaata ttgctaagag tattacagat acattttaaa ttttaaaagg
     8221 taaatcgatc gttgattggc ataaagagat gaaatttgac gttcggattt accctttttt
     8281 tgtggggatt tatagggatg ttaagatatt ttgttaaatt ttttgcgaaa ataaaaaata
     8341 aataacacta gctattgtaa atgtatatgc aatcttgtat aatttttgaa tgattgataa
     8401 tcattttcaa taggaggaaa ttatgaaaaa actattatta ccattaataa ttatgttatt
     8461 agtgttagct gcgtgtggga accaaggtga aaaaaataac aaagctgaaa ctaaatctta
     8521 taaaatggac gatggcaaaa cggtagatat tccgaaagac cctaaacgca ttgcagtagt
     8581 tgcgccaaca tatgctggtg gacttaaaaa attaggtgca aacattgtag ctgtaaatca
     8641 acaagtcgat caaagcaaag tattaaaaga taaatttaaa ggtgttacaa aaattggtga
     8701 tggcgatgta gaaaaagttg ctaaagaaaa gccagattta attattgtat actctactga
     8761 caaagatatt aaaaaatatc aaaaagtagc accaacagta gttgttgact ataataagca
     8821 taaatattta gaacaacaag aaatgttagg gaaaattgtt ggtaaagaag ataaagtaaa
     8881 agcttggaag aaagattggg aagaaacaac tgctaaagac ggtaaagaaa ttaaaaaagc
     8941 aattggacaa gatgcaacag tgtcattgtt tgatgaattt gataaaaaat tatacactta
     9001 cggcgataac tggggtcgtg gtggagaagt attatatcaa gcatttggtt tgaaaatgca
     9061 accagaacaa caaaagttaa ctgcaaaagc aggttgggct gaagtgaaac aagaagaaat
     9121 tgaaaaatat gctggtgatt acattgtgag tacaagtgaa ggtaaaccta caccaggata
     9181 cgaatcaaca aacatgtgga agaatttgaa agctactaaa gaaggacata ttgttaaagt
     9241 tgatgctggt acatactggt acaacgatcc ttatacatta gatttcatgc gtaaagattt
     9301 aaaagaaaaa ttaattaaag ctgcaaaata attcagctat ataagttagt gaaatgagag
     9361 tctgaaacat atcaatcttt tgatattgta ttaggctctt atttttatag ctagaaagtt
     9421 agatatttgt atttttttaa ataataagtg ccgttgttat cgttcaattt aattaatgat
     9481 agattagtat tattatagct aaagtagtat acctgagaaa atagctcaat gtatctcttt
     9541 attaataagt tatatcataa ttattttagt gcatacttta tggaagggat atcagggaat
     9601 ggctttcaat taaagaagag gtttaaaagg attacaacag aatgttatga ttttgtagaa
     9661 agatatataa caacgtttta taaaaacata atattgttaa tggaaaatga aatgtaaggg
     9721 ggatttcgag tgactaagaa agtttatttt aaccacgatg gtggtgtaga tgatttagta
     9781 tctctatttt tattattaca aatggaaaac gttcaattga taggggtcag tacaattggt
     9841 gctgattgtt atttagagcc atctttgagc gcatcagtaa aaattattaa tcgtttttca
     9901 aatgaagata ttcaagttgc gccatcatat gaacgaggaa aaaatccatt tcctaaagaa
     9961 tggcgtatgc atgccttttt tatggacgca ttgccaattt taaatgagcc agtcaaacat
    10021 gttgcttcaa atgtgagcga caaagaagcc tttgaagaca ttattcaaac tttaaagaga
    10081 caatcagaaa aagtaacatt attatttaca ggcccgctta cagatttagc aaaagcacta
    10141 caaaaagatt catctatcgt tcagtatata gaaaaattag tttggatggg tggcaccttt
    10201 ttaccaaaag gaaatgttga agaacctgag catgatggtt ctgcagaatg gaatgcatat
    10261 tgggatccag aagcggttaa aattgttttt gatagcgata tagagattga tatggttgct
    10321 ttagaaagta cgaatcaagt accgctaacg ttagatgtta gacaaagatg ggcgaatgaa
    10381 cgccaatata cgggcattga ttttttagga gtaagttatg cagcggtacc accattaaca
    10441 cactttataa caaattctac ttacttttta tgggatgttt taacgactgc ttatattggt
    10501 aacaaggact tggttcattc aattgagaaa aaagtcgatg taataagtta tggaccaagt
    10561 caaggtaaga catttgagtg taaagatggg cgcaaaatta atgtcataaa tcatgtagat
    10621 aacaacgcat tttttgatta tataactgca cttgctaaaa aagtaaatta acagctgtgt
    10681 agaataatta aggttttaat ttatatagaa caacttattg taaacttttc atttcttaaa
    10741 gtttacaatg gtgctataat aatggtcatg aaatacgaaa ggaagtaaaa aatgacaaca
    10801 aaacagttag tatatacagc tttaatgaca gcgattatcg ctattttagg attggtaccg
    10861 gtaattccac taccattttc ttcagtacca attgtacttc aaaacattgg tattttctta
    10921 gcaggtgtga ttttaggacg taaatatggc acattaagtg ttatcgtctt tttattatta
    10981 gtagttgctg gcttgccatt gttatcaggt ggtcgcggtg gcatcggtgt attcgcaggt
    11041 ccttcagcag ggtttttact attatatcca gttgtagcat tcatgattgg ggcgattcga
    11101 gatagattca tcaatgaaat taatttctgg attttattcg ttggtatttt agtttttggt
    11161 gttatagcat tagatgttat tggtacattg attatgggca tgattattaa cataccattt
    11221 acgaaagcta tttcaatttc attagcttat ttgcctggtg atatattaaa agcaattgta
    11281 gcaagtttga ttggtacagc tttacttaat cactcgcagt ttcgtcaaat tatgggaata
    11341 aaataatcat atttaagata gtaaagtaat tgaataagtt gctttgaaat ttataaaagt
    11401 gaaaggagta ggtgtcaatg gctagtataa gtatgtcaga tatatattgt aacggcacta
    11461 tatttgaaaa tgacgacgag cagttgattt atttaacgcc ttcttttcca caacgataca
    11521 caagtaacac atggatatat aaaaagacgc ctacccaaga gcgatggctg aaagacttag
    11581 aacgtcaaca tcaattacat acaaatcaag gttcaaatca ttatgcgttt agtttcccgg
    11641 aaaatgaaca acttgataat cattggatgg ctatgtttaa agatatgaat tttgaactag
    11701 gtattatgga attgtatgcc atagaaagtg atgcgcttgc caatttgccg cgtaactctg
    11761 acgttgaaat tgccatcgtt gacgagtcgc atatagatgc ctatttaaaa gttgcatatc
    11821 agtttagttt gccatttgga aaagactatg cagatgcaca tgaagaaatg gtaagggaac
    11881 attatcaaaa agatgtgatt aaacgcttag tagcttattt aaataatgaa cctattggcg
    11941 ttgtagatgt cattgaaagt gaaaattaca ttgaattaga tggatttggt gtattagaac
    12001 aatttcggca ccaaggaatt ggatctacaa ttcaatcgtt gataggtgaa tacgccatat
    12061 caaaaaatca caaaccaatc atattagttg cagatggtga agatacagca aaagatatgt
    12121 atgcaaagca aggttatgtc tatcaatcgt tttgttatca aatattaaaa gaagatattg
    12181 gaaattaacg attgatgata ggtttgtatt agaataaaaa agcagtaagc tggtttcgag
    12241 tagaaatcaa cttactgctt tttaaattgt tttgagctac ttatacttat aaaaatagtg
    12301 cgtttaaatt gttgattcat gtagaatatc gttcattatg acacactata atgaatatgt
    12361 tattgttcag aatcaatgat acgttctgga tgactgtata tattaaagcc accatttcga
    12421 ataaatccaa ctgccgtaat atttaggtca ttagctaagg ttacagcaag cgttgtcgga
    12481 gctgatttag ataaaatgac gccaacacca atttttgcgg ctttaattaa aatttctgat
    12541 gaaatacgtc cactaaaaat taatacttta tctcggacag taatatgtcg ctgaatacaa
    12601 aatccatata atttatctag agcgttatgt ctaccaatgt cttgtcgatg tacaaaaaat
    12661 gtcaaaccat cgcttatagc agcattatgt aagccacctg tttcttggta aatatgactt
    12721 gcactttgta atcgagtcat catgttaata atttgcattg gagttaaagt gattttagac
    12781 atagatgttt tagcgatagc agcatcattt tgaaaataaa actcacgact ctttccgcaa
    12841 caagatgcaa tcattcgttt tgtggaatat tgaaagcgat cgcctaaatc tttattaagt
    12901 tcaacatggg caaaaccttt actatcatca atcagtacag attttaattc atctcgcttt
    12961 aaaatggcac cttccgaagc cagaaatcca atgactaact cctcaaggtt tgttggactg
    13021 catataacag tcgcaaattc ttcaccattc accataattg taagtggaaa ttctgtcaca
    13081 tattgatctg ttgtattgaa taattttcca tcttcatatc taacaattgg ttgacctaaa
    13141 gatacatctt tgttcattat ctaacccctt taattagctt aaactttatt ttaaagcaat
    13201 ttgcttaaaa ttttaacata tttgcttaag tttgaaattt gattgataaa aattaatagc
    13261 gagcaatctg tttgatttaa attgaattcg agaatataca tactagggca tcaattaata
    13321 aatatcaatc ttatgcaaat ttgacaattg tttgaatcaa tatataaaca ggcaacggtt
    13381 cttttcaaat ataatagtaa gtgtataatg aaaatgtaaa tattattaaa aatgggggtt
    13441 cactcaatga aattgaaacg tttatttgct gttgtgattg caatgctttt agtattagct
    13501 ggttgctcta attctaacga taataatgaa agtaaaaaag atgacgcaga caatggtaag
    13561 aaacaagaga ttcaagttgc agcggcagca agtttaacag atgtaaccaa gaaattagct
    13621 tcagaattta aaaaagagca taaaaatgct gatattaaat ttaactatgg tggatcaggg
    13681 gcattaagaa aacaaattga atcaggcgca cctgttgacg tatttatgtc tgcaaatact
    13741 aaagatgtag atgcattaaa agacaagaat aaagcgcatg atacatataa atatgcgaaa
    13801 aatagtctag tattaattgg tgataaagat tcaaattaca cttcagtaaa agacttaaaa
    13861 gacaatgata aattagcatt aggtgaagtg aaaactgtac cagcaggaaa atatgcgaaa
    13921 cagtatttag ataacaataa cttatttaaa gaagtcgaaa gtaaaatcgt ttatgctaaa
    13981 gatgtaaaac aagtattaaa ttatgttgaa aagggtaatg cgaaacaagg ttttgtgtat
    14041 aaaactgact tatataaaca aaataaaaaa attgatactg taaaagtaat taaagaagta
    14101 gaacttaaga agccaatcac atacgaagct ggtgctacat cagatagtaa attagcaaaa
    14161 gagtggatgg aattcttaaa atcagataaa gctaaagaaa tactaaaaga ataccacttt
    14221 gcagcataag gagttgtaat ccatgcctga cttaacacct ttttggatat caatacgagt
    14281 tgctgtaatc agtacgatta ttgtaacggt tttaggtatt tttatatcta aatggttgta
    14341 tcgtcgtaag ggttcgtggg ttaaattatt ggaaagttta ttgatattac ctattgtttt
    14401 gccgccaacg gtattaggtt ttattctatt aatcatcttc tcgccaagag gaccaatcgg
    14461 tcaattcttt gcgaatgtac tacatttacc tgtagtgttc actttgacag gtgctgtgat
    14521 agcatctgtc attgttagtt ttccactaat gtatcaacat actgtgcaag gcttcagagg
    14581 tatagacacg aaaatgatta atacagctag aacgatggga gcaagtgaaa cgaaaatttt
    14641 cctcaaatta attttaccat tagctaaacg ctctatttta gcaggtataa tgatgagttt
    14701 tgctcgtgca ttaggtgagt ttggtgctac attaatggtt gcaggatata ttccaaataa
    14761 aacgaataca ctacctttag aaatatactt cttagtggaa caaggtagag aaaatgaagc
    14821 gtggttatgg gtattagtgc tagtcgcatt ctctattgtg gttatatcta caattaattt
    14881 attgaataaa gataaatata aggaggtcga ctagatgctt aaaatcaatg tgaaatatca
    14941 attaaagaac actttaattc gcatcaatat agatgatact gaaccaaaaa tttatgcagt
    15001 tcgtggtcca tctggcattg gtaaaactac tgttttaaat atgattgccg gattacgtaa
    15061 agcagatgaa gctattatcg aagtgaatgg gcaattactt actgatacgg caaaaaacgt
    15121 gaatgttaaa attcaacaac gacgtattgg atatctgttt caagactacc aattgtttcc
    15181 taatatgacg gtctataaaa atattacttt tatggctgaa ccatctgaac acatcgatca
    15241 attaattcaa actttaaaca ttgatcattt gatgaaacaa tatcctatga cattgtcagg
    15301 tggagaggca caacgtgtag cacttgcacg tgcacttagc acgaaaccag atttaatttt
    15361 attagatgaa cctttttcta gtttggatga tactacaaaa gatgagagta ttacattagt
    15421 taaacgtatt ttcaacgaat ggcaaatacc aatcatattt gtgacacatt caaactatga
    15481 agcagaacaa atggctcatg aaattattac aattgggtaa tcatttattt gccattaaag
    15541 agtttagaac gtatttaaaa ttgtagaagt gaatgcttct atcagcattt taatgatgtt
    15601 ttaaactctt ttttaggggc agtttttttg agagacattg acgcgcgtca tataatgaaa
    15661 gtaatgataa aaagaaagga taacttaatg tgagtcaaga acgttattca aggcaaattt
    15721 tatttaaaca aataggtgaa ataggtcaaa gcaaaataaa tcaaaaatgt gcgttgatta
    15781 ttggtatggg cgcattaggt acacatgtgg ccgaaggact tgttagagca ggcattgcca
    15841 aactaatcat tgttgataga gattatattg aatttagtaa tttacaaaga caaacattgt
    15901 ttactgaaga agatgctttg aaaatgatgc ctaaggtggt tgcagctaaa aagcatttgc
    15961 tagcgttacg tagtgatgtt gatattgatg attatattgc ccatgtggat tattattttt
    16021 tggaaacaca tggacaggac gttgacgtta ttattgatgc aaccgataac tttgaaacac
    16081 gacaactgat taatgatttt gcatataaat atcgtatacc ttggatttat ggtggtgttg
    16141 tacagagtac atatacagaa gctgcattta tacctggtaa aacaccttgc tttaactgtt
    16201 tggtaccaca attgccagca ttaaatttaa catgtgatac agtaggggtc attcaacctg
    16261 ccgtgacgat ggcaacaagt ttacaattaa gagatgcgat gaaagtatta acggaacaac
    16321 caattgacac aaaaataact tatggcgata tttgggaagg tagtcattat tcatttggtt
    16381 tcagtaaaat gcaacgttca gactgtacaa cttgtggaga tgtaccaagt tatccgtatt
    16441 taaacaagaa tgaacaacgt tatgcaacat tgtgtggtag agacactgta cagtatgaaa
    16501 atgcatcaat tacacacgac attcttgttc aatttttaaa acaacatcag ttaaattatc
    16561 gcagtaattc gtatatggtt atgtttgaat ttaaaggaca ccgcattgtt gcttttaaag
    16621 gtggaaggtt tttaatacat ggcatgacac gcacatcaga tgccacacat ctaatgaatt
    16681 tattgtttgg ataaaaaaag ataagacaaa aggagtgtaa tattatgggc gaacatcaaa
    16741 acgttaaatt gaatcgtaca gttaaagcag ccgtactaac ggtatcagat actagagact
    16801 ttgatacaga taaaggtggt caatgcgtgc gccaactatt acaagcagat gacgttgaag
    16861 tgagtgacgc acattataca attgtgaaag atgaaaaagt agccatcacg acgcaggtga
    16921 agaagtggtt agaagaagat attgatgtca tcattacgac tggtggaaca ggtattgcac
    16981 aacgtgatgt gacgattgaa gcagtaaaac cacttttaac taaagagata gatggctttg
    17041 gggaattgtt tagatatttg agttatgttg aagatgttgg cacgcgtgca ttattgtctc
    17101 gtgctgtagc aggtacagtt aataataaat tgatattttc gattccagga tcaacaggcg
    17161 cagttaaatt agcattagaa aagctcatta aaccagaatt aaatcatctg attcatgagc
    17221 ttacaaaata atttattgat ttgattggcg ttgaaaatct ccagatttac cgccagactt
    17281 gctttcaagg taggtttcgc caataatcat acctttatca actgctttcg tcatgtcgta
    17341 aatggttaaa gccgttgctg atgcagcggt taaagcttcc atttcaacac cggttttgcc
    17401 agttgtagag acagttgttt gaatgtttaa agtataaagg ggtgcatttg tttcatccca
    17461 gctgaagtga acatctatgc cagtcaatgg taatggatgg cacatcggaa taagtgttga
    17521 tgtatttttg gcagccataa taccagcgat ttgagcagtg ttcaatacat tgcctttggt
    17581 attggtgtta ttagaaatct gcttaaaaat agtttcattt acagtaatgc ttgaatgagc
    17641 gacagcagtt ctttttgtaa tttgtttgtc tgatacatcg accattttgg cgtggccttg
    17701 ttgattaata tgagtaaact cagtcatttt acccctccta gtgcatctag tatatcatga
    17761 aaaaataaaa gttttggaga tgatttttaa tggtagtaga aaaaagaaac ccaatcccag
    17821 ttaaagaagc aattcaacgt atcgttaatc agcagagttc aatgccggca attacggtag
    17881 cacttgaaaa aagtctaaat catatcttag cagaagatat tgtagctact tatgatatac
    17941 caaggtttga taaatcacct tatgatggtt ttgcaattcg cagtgttgat tcacaagggg
    18001 caagtggtca gaatcgcatt gagtttaaag tgattgatca tattggtgca ggttcagttt
    18061 ctgataaatt agttggggat cacgaagcgg tgcgtattat gactggagca caaataccta
    18121 atggcgcaga tgctgttgtt atgtttgaac aaacgattga actagaagat acatttacaa
    18181 ttcgtaaacc attttcaaaa aatgaaaata tatctttaaa aggtgaagaa acaaagacag
    18241 gcgatgttgt tctaaaaaaa ggacaagtaa ttaatccagg ggctatcgcg gtccttgcaa
    18301 catatggcta tgcagaggtt aaagttatta agcaaccgag tgtcgctgtt attgcaacag
    18361 gaagcgaatt attagatgtt aatgatgtat tagaagatgg aaaaattcgt aactctaatg
    18421 gcccaatgat tcgtgcctta gcagaaaaat taggtcttga agttggtatt tacaaaacac
    18481 aaaaagatga tttagatagt ggcatccaag tcgttaaaga agctatggaa aaacatgata
    18541 tcgttattac aacgggcgga gtttctgttg gagattttga ctatttacct gagatttata
    18601 aggctgtaaa ggcggaagtg ttatttaata aagtagcaat gcgtcctggt agcgtaacaa
    18661 cggttgcatt tgtagatgga aagtatttgt ttggattatc tggaaatcca tcagcttgtt
    18721 ttacaggatt tgaactattt gtgaaaccag ctgttaaaca tatgtgtggc gcactagaag
    18781 tcttcccgca aataattaaa gcaacattaa tggaagattt taccaaggca aacccattca
    18841 cacgatttat acgtgctaaa gcaacgttaa caagtgctgg agctactgta gtaccttcag
    18901 gattcaataa atcaggtgcg gttgtagcga ttgcacatgc taactgtatg gtcatgttac
    18961 caggagggtc acgtggtttt aaagcggggc atacagtaga tattatattg actgaatctg
    19021 acgctgctga agaggaactt cttttatgat tttacaaatt gtaggttaca aaaagtctgg
    19081 taagacaaca ttgatgaggc atattgtctc tttcttaaag tcacatggtt atacagttgc
    19141 tactattaaa catcatgggc atggtaagga agatattcaa ttacaggatt cagacgtcga
    19201 tcacatgaag cattttgaag cgggggcaga tcaaagtatt gtacaaggtt ttcaatatca
    19261 gcaaactgta acacgtgtag ataatcaaaa tcttactcaa attattgaaa aatctgttac
    19321 aattgacacc aatatcgtat tagttgaagg ctttaaaaat gctgattttg aaaaagtcgt
    19381 agtctatcga aatgaagaag agttgcaagt attacaacaa ttgtcgaatg tttgttatag
    19441 cattaatgta agggagcatg aagattttac agcatttgag caatggttat taaataaaat
    19501 taaaaatgat tgtgatacac aattaacata gaggattgaa atgaatgaaa caatttgaaa
    19561 tcgtgacaga accgatacaa acagaacaat atcgtgaatt cactataaat gaatatcaag
    19621 gtgcagtagt tgtttttacc ggtcatgttc gcgaatggac taaaggcgtc aaaacggaat
    19681 atttagaata tgaagcgtat attccaatgg ctgaaaagaa attggcacaa attggagatg
    19741 aaataaatga aaaatggcct ggaacgataa cgagtattgt tcatagaata gggccattac
    19801 aaatttcaga tatcgctgta ttaattgcgg tttcttcacc gcatcgtaaa gatgcctatc
    19861 gagcaaatga atatgcaatt gagcgtataa aagaaattgt tccgatttgg aaaaaagaaa
    19921 tttgggaaga tggttcaaaa tggcaagggc atcaaaaagg gaattatgaa gaagcgaaga
    19981 gggaggaata agagagatga aggtacttta cttcgcagaa attaaagata tattacaaaa
    20041 agcacaggaa gatattgtgc ttgaacaagc attgactgta caacaatttg aagatttatt
    20101 gtttgaacgt tatccgcaaa tcaataataa aaagtttcaa gttgctgtaa atgaggaatt
    20161 tgtacaaaaa tcggatttca ttcaacctaa tgatactgtt gcattaattc caccggttag
    20221 tggaggttaa gggagcatga aagcaataat tcttgcaggt ggtcattcag tgcgatttgg
    20281 taagcccaaa gcttttgcgg aagtgaacgg tgagaccttt tatagtagag taattaagac
    20341 attagaatca acaaatatgt tcaatgaaat tattattagt acaaatgcgc aattggcaac
    20401 gcaatttaaa tatccaaatg ttgttataga tgatgagaat cataatgata aaggtccatt
    20461 agcaggaatt tatacaatca tgaagcaaca tcctgaagaa gaattgtttt ttgtcgtttc
    20521 tgttgataca ccaatgatta ctggtaaagc tgtaagcacg ttgtatcagt ttttagtttc
    20581 tcatcttatt gaaaatcatt tagatgtcgc agcttttaaa gaagatggac gttttattcc
    20641 aacaattgca ttttatagtc cgaatgcatt aggcgctata actaaagcac tacattctga
    20701 taattacagt tttaaaaatg tatatcatga attatcaacg gattatttgg atgtaaggga
    20761 tgtagatgcg ccctcatatt ggtacaaaaa tataaattat cagcatgatt tggacgcttt
    20821 aattcaaaaa ttgtaagctg ttaggaggtc cacaaatggt agaacaaata aaagataaac
    20881 taggacgtcc catccgtgac ttacggttat ctgtgacaga tcggtgtaac tttaggtgtg
    20941 attattgcat gcctaaagag gtatttggag atgatttcgt atttttacct aaaaatgaac
    21001 ttttaacgtt tgatgaaatg gctagaatcg ctaaggtata tgcagaatta ggtgtaaaaa
    21061 aaatacgcat tacaggtgga gaaccattga tgcgacggga tttagatgta cttatagcta
    21121 aattaaatca aatcgatggt attgaagata ttggtttgac tacaaatggt ttgttattaa
    21181 aaaagcatgg acaaaagtta tatgatgctg ggctgcgcag aattaatgtc agtttggatg
    21241 ctattgatga tacgctattt caatcaatca ataatcgtaa tattaaagcg actacgattt
    21301 tagaacaaat tgattacgcg acgtctattg gtttgaatgt aaaagtaaat gttgttatac
    21361 aaaaaggtat taacgatgat caaatcatac caatgcttga atattttaaa gataaacata
    21421 tagagattcg atttatagaa tttatggatg ttggtaatga taatggatgg gatttcagta
    21481 aagttgtaac taaagatgaa atgcttacaa tgatagagca gcactttgaa atcgatcctg
    21541 tagaaccaaa atattttggg gaagtagcaa aatattatcg ccataaggat aatggtgttc
    21601 aatttggttt gattacaagt gtttcacaat cattttgttc tacatgtaca cgcgcaaggc
    21661 tgtcatcaga tgggaagttt tacggatgtt tatttgcaac tgtcgatgga tttaacgtta
    21721 aagcgtttat tcgttctggc gtgaccgacg aagaattaaa agaacaattt aaagctttat
    21781 ggcaaataag agatgatcga tattcagatg agagaactgc tcaaacagtt gccaatcgtc
    21841 aacgtaaaaa gataaacatg aattatattg gtggttaatg tgtagggacc actacatatt
    21901 aaatcattag agatgtttta atatttctgt cttactccct aaaatacaat attatttatt
    21961 aaagtaaaaa cggtcatatc tatgccagat ttaatagaaa tgatcgtttt taaagttttt
    22021 acaagttggc ggggccccaa cacagaagct gacagaaagt cagcttacaa taatgtgcaa
    22081 gttggcgggg ccccaacata gagaatttca aaaagaaatt ctacagacaa tgcaagttgg
    22141 ggaacggggc cccaacacag aaggtgacga aaagtcagca tacaataatg tgcaagttgg
    22201 cggggcccca acatagagaa tttcaaaaga aattctacag acaatgcaag ttggggatca
    22261 acgaaataaa ttttatgaga atatcatttc tatcccactc ttaagaatca ctacataata
    22321 aatctttagt ggttctttaa cattgatgtc acactccatg ccattgagtt gtaatatatc
    22381 ttttttaggt ataaatgttg tcgaataaac aacaagttgt ccaaaagata taaatctaaa
    22441 caagatatag ccagcaattt aatatttgta atagataaaa tgctaagttt gatatataat
    22501 aaatttaagt aattgtataa taatatgaat tacaaacatc taagaagaaa cataggaggc
    22561 atcatattat gagtaataaa gttcaacgtt ttatagaagc agaaagggag ttaagtcagt
    22621 taaagcactg gttaaaaaca acacataaga tttcaattga agaatttgta gtccttttta
    22681 aagtgtatga agctgaaaag attagcggta aagaattgag ggatacatta cattttgaaa
    22741 tgctatggga tacaagtaaa atcgatgtga ttatccgtaa aatctataaa aaagagctta
    22801 tttctaaatt gcgttctgaa acggatgaaa gacaagtatt ctatttctat agtacttctc
    22861 aaaagaaatt gttagataaa attactaaag aaatagaagt gttaagcgtt acaaactaaa
    22921 aacttaaaaa gcatgccaat cagtacatca taattgcgtc ttggggacag acaaatgatg
    22981 aatagagatt ggcatgcttt ttatttttga atataaatat ttagttcatg gcatttctag
    23041 ttacatgacg tccatgaatt aagaagtaaa caagcatagt aatgattgct aaagcggcca
    23101 taaagccgaa gatttcacta tatgaaaaca tatgagtaaa taacccaagg aatgatggac
    23161 cgaagccgac acctgcatct agaccaacgt aaaaagtaga tgtcgcgata ccatatttaa
    23221 tcgggggtga gacttttatc gcaatagatt gcattgcaga tgataaattt ccatacccta
    23281 aacctaggca agcaccagca agtaatatta accagctttg atagcttgaa attaagcata
    23341 caaatgaaag gaaaagcatg ataaatgctg ggtagacaat aatattttca tttttatcat
    23401 ccatcaatct accagcaata ggtctagtaa ttaacgatgc tatagcatag caaataaaga
    23461 aatagcttgc tgcagtgact aggtgtcgct ctaaagcaaa tgcttgtaaa taagttagga
    23521 tggacgcata ggtaacgcca attaaaagca taattacagc aacaggaatg gcctcttttg
    23581 caataaattg atgaatacta aatcttggtt tatcaatgac attagtttca gttttgttat
    23641 ttgttacttc gaaatcaact tttataaata atgagataat gagtccgagt atgcctaata
    23701 tgacacaaat aataaacagt aagtcaattg cgtattttgt aataagtaac atgcctagaa
    23761 atgggccaat cgctgtacct aatactaaac ttaaggaaaa taaactgatg ccttcacttt
    23821 ttctattaac aggggtaacg tatgccgcaa tagtacctgt tgcagttgtc acaactgcag
    23881 ttgcgatacc gtttatgaga cgtacaaaga ttaaaaaagc taaagatcca tcaataaaat
    23941 aaagtaattg cgtgataatt aaagcaatta aaccaataaa taataatcgt ttaggtccga
    24001 tttgatttac aaatttacct gtagcaaatc gaccaattaa agacccgaca ataaatagtc
    24061 ctacaactaa tccggctaat gaatcggaag cgtcgaaagc ttgcttacta tagccagcaa
    24121 taactacaag taacaaatac atgcataggt atacgataaa gttgactaaa aagttaattg
    24181 taaaactttt ggtgaaaata ggtgatttat ttaatgagcc ttgattagtc attgtgtgcc
    24241 tcccttaata atatttcatg aatttgattc atcacattta tggtttcttg catttgatct
    24301 tgcgaaatgt ttgttttggc aactactgct tgttggagct tttgtacatc aagacaaaca
    24361 atctcatata attcatgccc tttaggtgtt aaagttaaaa acttttgtcg tttatcctct
    24421 aaactgtttt ctactgttat aaggtcattt tctattagag cttttaaagt ttttcttgca
    24481 gtaggctttt caattgcacg tctatgtgaa atttcaatga gagtagtggg ctgatgttta
    24541 gcgatatcgc gtaagattaa ccattggcca taatatatat tgtgtttttc taaaatcggc
    24601 tcggctaatt ttaaataggg gcgatatatt gttataaaac tattgaaaaa ttcttgtgat
    24661 agcatagtga catctcctaa gacaaaatag ttagcttagc taaccttttt acaacaatag
    24721 taattataaa acgggagcaa ttagaaatca atatataatt attaagagca aaaataatta
    24781 tactttgtta aaataagcgt aattacatgt aaataggggg atactaatga tattgaaatt
    24841 tgatcacatc attcattata tagatcagtt agatcggttt agttttccag gagatgttat
    24901 aaaattacat tcaggtgggt atcatcataa atatggaaca ttcaataaat taggttatat
    24961 caatgaaaat tatattgagc tactagatgt agaaaataat gaaaagttga aaaagatggc
    25021 aaaaacgata gaaggcggag tcgcttttgc tactcaaatt gttcaagaga agtatgagca
    25081 aggctttaaa aatatttgtt tgcgtacaaa tgatatagag gcagttaaaa ataaactaca
    25141 aagtgagcag gttgaagtag tagggccgat tcaaatggaa agagatacac ataaagatgg
    25201 taaggtaaag tggcaattgc tttatataat gaatcaggat gatgatgaaa ttaagccacc
    25261 attttttatt caatgggaag aaagtgattc catgcgtact aaaaaattgc aaaaatattt
    25321 tcaaaaacaa ttttcaattg aaactgttat tgtgaaaagt aaaaaccgat cacaaacagt
    25381 atcgaattgg ttgaaatggt ttgatatgga cattgtagaa gagaatgacc attacacaga
    25441 tttgatttta aaaaatgatg atatttattt tagaattgaa gatggtaaag tttcaaaata
    25501 tcattcggtt atcataaaag acgcacaagc aacttcacca tattcaattt ttatcagagg
    25561 tgctatttat cgctttgaac cattagtata aatatacgta agtgctatga gcgagaatgc
    25621 ccatatgaat aatgacaagc acaatggaaa gaatcgttaa tatattattt aatcgtgatg
    25681 acttaattaa aatgaaaaag attgataata taaatgtgaa aaagataagt ataacccgta
    25741 aactaaagta attcacggtg agaggttgac tcaatgtcat aatgattgca acgatgttca
    25801 taattataaa tagacttaaa ataattgttc tcatatcaaa cacctcattg ttagattatt
    25861 gacattataa caggggtaat tgtatatgaa cattaatgtg gttgcttgag gaaaaattta
    25921 ttcattgaag tcaagttggt tcattttaga aatgaatatc gtgttagatg atgaaagtat
    25981 attgaagtat aggtaactag ttgaaaagta ttaattgtac gataacatta aatttaacac
    26041 gaaacataga tataaaatga ttcacaatta aaatgggtaa atttgaactt gctaaactat
    26101 taattggagc atggacattt caaaaataag agttcaaatc ttacacaagc tctgaatcga
    26161 cactataaga tacaaactgt ataattaaag gtattgttaa atagaaggag atatcataaa
    26221 tcatggaaaa gatgcatatc actaatcagg aacatgacgc atttgttaaa tcccacccaa
    26281 atggagattt attacaatta acgaaatggg cagaaacaaa gaaattaact ggatggtacg
    26341 cgcgaagaat cgctgtaggt cgtgacggtg aagttcaggg tgttgcgcag ttacttttta
    26401 aaaaagtacc taaattacct tatacgctat gttatatttc gcgtggtttt gttgttgatt
    26461 atagtaataa agaagcgtta aatgcattgt tagacagtgc aaaagaaatt gctaaagctg
    26521 agaaagcgta tgcaattaaa atcgatcctg atgttgaagt tgataaaggt acagatgctt
    26581 tgcaaaattt gaaagcgctt ggttttaaac ataaaggatt taaagaaggt ttatcaaaag
    26641 actacatcca accacgtatg actatgatta caccaattga taaaaatgat gatgagttat
    26701 taaatagttt tgaacgccga aatcgttcaa aagtgcgctt ggctttaaag cgaggtacga
    26761 cagtagaacg atctgataga gaaggtttaa aaacatttgc tgagttaatg aaaatcactg
    26821 gggaacgcga tggcttctta acgcgtgata ttagttactt tgaaaatatt tatgatgcgt
    26881 tgcatgaaga tggagatgct gaactatttt tagtaaagtt ggatccaaaa gaaaatatag
    26941 cgaaagtaaa tcaagaattg aatgaacttc atgccgaaat tgctaaatgg cagcagaaga
    27001 tgaaaacatc tgaaaagcaa gctaaaaaag cgcaaaatat gattaatgat gcgcaaaata
    27061 aaattgctaa aaatgaagat ttaaaacgag acctagaagc tttagaaaag gaacatcctg
    27121 aaggtattta tctttctggt gcactattaa tgtttgctgg ctcaaaatca tattacttat
    27181 atggtgcgtc ttctaatgaa tttagagatt ttttaccaaa tcatcatatg cagtatacga
    27241 tgatgaagta tgcacgtgaa catggtgcaa caacttacga tttcggtggt acagataatg
    27301 atccagataa agactcagaa cattatggat tatgggcatt taaaaaagtg tggggaacat
    27361 acttaagtga aaagattggt gaatttgatt atgtattgaa tcagccattg taccaattaa
    27421 ttgagcaagt taaaccgcgt ttaacaaaag ctaaaattaa aatatctcgt aaattaaaac
    27481 gaaaatagat taacgactga aatctgaacg ctcataagac tgtcatttgc gttcagattt
    27541 ttttacacaa tatagaatgg ttgagtaaaa tatttttgaa tatagtgaaa gagggggaag
    27601 tactgtgata aaaaagctat tacaattttc tttagggaat aagtttgcta tctttttaat
    27661 ggttgtttta gttgtcttgg gcggtgtata tgcgagtgct aaattgaaat tagaattact
    27721 accaaatgta caaaatccag ttatttcagt tacaacaaca atgccgggtg caacgccaca
    27781 aagtacccaa gatgaaataa gtagtaaaat tgacaatcaa gtaagatcat tggcatatgt
    27841 gaaaaatgtt aaaacgcaat ccatacaaaa tgcttcaatt gtaacagttg aatatgaaaa
    27901 taatacagat atggataaag cagaagaaca gcttaaaaaa gaaatcgata aaattaaatt
    27961 taaagatgaa gttggtcaac cagaattaag acgtaattcg atggatgctt ttccggtttt
    28021 agcatattca ttttcaaata aagagaatga cttgaaaaaa gtaacgaaag tactgaatga
    28081 acaattaata ccaaaattgc aaacggtaga tggtgtgcaa aatgcgcaat taaatgggca
    28141 gacgaaccgt gaaatcaccc ttaaatttaa gcaaaatgaa cttgaaaaat atgggttgac
    28201 tgctgatgat gtagaaaact atctaaaaac ggcaacaaga acaacgccac ttggattgtt
    28261 ccaatttggt gataaagata aatcaattgt tgttgatggt caatatcaat ctgttgatgc
    28321 ttttaaaaac ataaatattc cattaacgtt ggcaggagga caagggcaat ctcaatccca
    28381 aagtgacaat aaacaaaatt cagccatgtc agacgttaat caggcatcac cacagcaaaa
    28441 ttcaaaagcg tcagcaccaa ataatataag tggtatgccg acagcgaaac taggagattt
    28501 agctgatatt acagttggtg atgtgcgtac ttctatttct aaaacgaatg gaaaggatgc
    28561 ggttaatcta caaataacta aagctcaaga tgccaataca gttcaagtag ccaaagatgt
    28621 acaacgtaaa attgatacat tcgttgatga aaataaagat tttaatgtca caaaaacaat
    28681 ggatactgca aagcctgttg agaaatcact ttatacgatg gttgaaaaag catcattagg
    28741 tacaatcgtg gcaattatag ttattttgct gtttttaaga aacattcgta cgacggcaat
    28801 ttctattata tcgattccgt tatcacttct tatggcgctt attgctctga aattgagtga
    28861 tgtttcattg aatatactaa cgttaggtgc attaacagta gcgattggac gtgtgataga
    28921 cgattcgatt gtagttgttg aaaatattta tcgacgctta acagattcag aagaacaact
    28981 aaaaggtgaa aatttaatta tcagtgcgac aactgaagta tttaaaccaa taatgtcatc
    29041 gacactagtt actattatcg tcttcttacc acttgtgttt gtatcaggtt cagtaggcga
    29101 aatgtttaga ccttttgcat tggctattgc atttagttta ttagcatcgt tattagtgtc
    29161 aattacactc gttccagcgt tggcagctac actatttaaa aaaggcgtta aacgtcgtaa
    29221 taaacaacat caagaaggat taggtgttgt tagtacaact tataaaaaag tattacattg
    29281 gtcattaaat cataagtgga ttgtaattat attaagtaca ttaattttgg ttgcaactat
    29341 tgtatttgga ggaccgagac taggcactag ctttatttca gcaggtgacg ataaattttt
    29401 agctattact tatacaccga agcctggtga aacggagcaa gcagtgttga atcatgcgaa
    29461 agatgttgaa aaatatttaa aacagaaaaa gcatgtaaaa acaattcaat actcagttgg
    29521 cggtagtagt ccagtagatc caacgggtag tacaaatagt atggcaatca tggttgaata
    29581 tgataatgac acgcctaatt ttgatgtaga agcggataag gttattaaac atgcagatgg
    29641 ctttaaacat cctggagagt ggaaaaatca agatttagga acaggtgcag gtaataaatc
    29701 tgtagaggtt actgtaaaag gtccatcaat ggatgccata aaatcaactg taaaagatat
    29761 tgaacagaaa atgaaacagg ttaaaggact agccaatgtc aaatctgatt tatcgcaaac
    29821 atatgatcag tatgaaatta aagtcgatca aaataaagcg gcagaaaatg gtatttctgc
    29881 aagtcaactt gcaatgcact tgaatgaaaa cttaccagaa aaaacagtta cgactgttaa
    29941 agaaaatggt aaaactgttg atgttaaagt caaacaaaat aagcaaacag actggtcaga
    30001 agataagttg aataatataa ctttgaaaaa gccgactggt ggtacgatta aattgggaga
    30061 tatcgctacg ttagttaaaa caacgacacc aagtaaattg acgcaagaac aaggagatta
    30121 tgcaacgacg gtatctgcta aagtaacaaa taaagatgtg ggtggcacaa cacgacaagt
    30181 gatgtctaaa ataaataatt tggacaaacc gaataatgta aaggttaata tcggtggtgc
    30241 atcagatgat attaacaatg caatgacaca attagccttt gcaatgttag ctgcaatcat
    30301 tatcgtatat ttaatcctag ttattacatt taaaggtggc ctagcaccat ttacaatttt
    30361 attctcttta ccatttacag ttatcggtgt aattattgca ctattaatca caggagaaac
    30421 aatatcagta ccaagtttaa ttggtatgct aatgttaatt ggaatcgtag taacaaatgc
    30481 cattgtgtta atagaccgtg ttattaataa tgagcaacag ggcatggaga tgaaagaagc
    30541 attaatcgaa gcaggcggta ctagaattag accgatatta atgacggcga ttgcaacaat
    30601 tggtgcatta gttcctttgt tatttggtca agatagctcg attcttattt cgaaaggatt
    30661 agctgctaca gttattggtg gtttaatttc atcaacttta ttaacacttg tagttgtacc
    30721 agtaatatat gaaattctct ttactttaaa aaaacgattc actaaacgat agataatata
    30781 aaagccataa aagcggtcat gatattgact acttttatgg ctttttaaaa tttgaaattt
    30841 tagaccttat ttcacgtgcg atttaatcga tgtgttaata aaacaatact ggcattcaac
    30901 atgtaatccg ataataaaat aaggttgcaa ttatgttatt gcatattttg tttattactt
    30961 agaataatac gatggttaag atgaaatata tgacaaagat aaataatgac attaaattca
    31021 agatgtttac gccttttaga tcttttttaa gcattttaaa tcctgtcact tcgacattac
    31081 cgactaagag taataagacg ataaataccc accaattctc ttttggaaaa ctcatcgtcg
    31141 ttaatgctaa gacgaataat aacaacacac ccataataat tctcaaaatg cgagtaaata
    31201 caacttccat tttaaaaata ctaataaaac taacaattag ataaagtatt gctagtaaaa
    31261 tcagataaaa ttgcatatga taacttcctc tccgtttcaa agggttcatg tagtacagca
    31321 gcgtagaaaa cataaatagc agtgttggga tagatatgcg tgtatgttgt taatgaaaca
    31381 ctaaatcata acacgacaac atcttgtgat tctagatgta attgagcgaa aaatgaagca
    31441 attcatcaat ataacgtatt cagagcaatg ctagtcaaat ttatgtaaca aaaagcattt
    31501 attgcttgaa tgatagatta ttaatcttgc atttgagcac gaacgtttct aatttcattt
    31561 tcaatatctt taagttgacg aacaagtgcg gaagtatcta aattcaaaga ttcccgtttt
    31621 tctatgtcac cttgaagacg aacataatca tatttaagtt cggctagttg gttttctaaa
    31681 gacatactat aacactcctt tgaaatataa ttaagtgcat ttaatgtaga ttatagcatg
    31741 aaacagtaga cttctaaaat atctgtggag tatgttaaag ttataaatta tgaagattat
    31801 tgaaagaagg gataacagtg acagaacaat ttactatgat tattttatta attgcgcttg
    31861 gttacttttt gaagcgcatt aattttataa aagcaacaga tagtcaagtc attgctacat
    31921 tagttcttaa tgtcacactc ccatctttag ttattgtgaa tttaaacagt gcagaattaa
    31981 aattgtcatt ctccatttta ccaattttaa tgattatata tggtattgtg gcgaaaatga
    32041 ttgctgtctg gttttttaga aaatatgata atcacatgcg tggatcagta ggtatgatga
    32101 ctggtgctat gaatattgga ttgtttgcat atccactaat tgaagtgata tggcctaaaa
    32161 cgggacttat ttattttgtt atggctgata taggtggtgc ctttgtgatg tttggaatta
    32221 cttactttgt aggtagctac tttagtgaag gtagtgatca attcgatttt aaatatttag
    32281 gtaagaaatt attacaatca gtgccactcg ttacatacat tgtaatgttt atattgaata
    32341 taagtcacat ccatttacca catgtggcaa tcgatttctt tagcatactt tcccatgcaa
    32401 atatgccgtt atcaatgatt ttgctaggtg tcatgttgaa ttttactctg gaaagaaagt
    32461 atatacctgc tactattaaa tatttatgtt tgcattatgg attagcatta gttgcgggat
    32521 tgttagtata tgtgttgtta ccagtatcag atgatatgat taagacgaca ttgttggtca
    32581 cttggatgtt cccggtaggt gttgcaatta tttcttatgg tattcagttt aaatatcgaa
    32641 cactgacatt tataggtatg acgaccaatt tgacaatcat tattagtatc attattttgt
    32701 acgtgtatca ggcagtgttt gtctagttta cacatttgaa aataaaatcc attttagtat
    32761 tcgtagaaaa tgagagcccc ctttaccaca atatgtaaat gtatattgtg aaaagggggc
    32821 ttttatttat gacatagttg ttcaactcat acattcaagt tgataatgca gtgcgttatg
    32881 cagctatcca cttcatatgt atggcgcata cttgatatat gaatatcatc aagattttaa
    32941 aagtatcgct aattttaaag tcttaaaacg catgattaat gtcatgatgt gttattgcgt
    33001 tttgttttta gagccacgta tttcctagga catatagagg aacattcaac gtaaaattcc
    33061 cgaaaattaa ttttaatcat tctataagtg aatggttatt ttcagaagtg attacatttt
    33121 tacgttacat gagtatttaa ctttctattt caaattacct agaattatag cagctatcac
    33181 gataataata atacctgccc aaatacccgt catctgacga cgatcttttc tttctcctaa
    33241 aatgaaaata ccgcctaagg ttgaaacgat aacaagtaac tgtgataatg agaaacttgt
    33301 agctacacca acttttggtt gagaatagaa catgaacaag ttaccaattc cccaaatcac
    33361 acctggcaat agattaagtg ctgttgattt aagtgatgtt ttatgattca tggataggat
    33421 aaagccacca attgccatac cgacagattg gaagaacaat gcatcagttc caccaacacc
    33481 aaagatgtca ccaagtacaa cgaaacctac atatccaact gtagatacaa ttaaaatacc
    33541 cattgctttt ttaaattcag gattatctga ttgacgttca tttttagctt taagtgaagt
    33601 aagtgctaca ccagtaacta ataatatcat ggcgattaaa ccaaagataa tttgagtcat
    33661 tgaagaccat tcacctaaaa agataacgct gaataatgtt gtaccaacta attgcatacc
    33721 tgtagaaact ggcatagtat ttgatacacc gattaaacta atagatttaa gctgattcgc
    33781 ttgtccaaac gcccataatg caccagaaat aagaccgaca ataattacag taggattatt
    33841 gaattttgcg aaaccagtaa taagtaaacc taatccaaca atgagtgcgc ctagcgtcgt
    33901 accacgaatt tggttgtaag gtccaccgcc gacgaacaca ttaataagaa cgacacttcc
    33961 ccagaataaa gcaggtaaaa gtgcgattaa gaaatcaaga aattgcaaga ctactcactc
    34021 tcctatacta tttatgcaag tgctcccctt aattgacact tgcatgttat tgtaaagcat
    34081 tctgccctaa tagtaaaaaa caactactta taattttcaa tatttacaaa aagtagaaaa
    34141 acccacaata catgctaatg atgtactgta gggtatttgc ttggtgtttt attcagggat
    34201 tgtttgatct tttacgttac gtgattcgaa tttgaattta atgtactcat agttgatatg
    34261 gaaacctaga ccattaaatc gttcgatgag tttattattt ttattacgaa ttttaaaata
    34321 aacttttcta atatcaaaat tatcaaaagc atattgcaat gcatacgcaa gcaactcgaa
    34381 agcgatacct ttcaaacggt aatctacatg agaactgaag tatttaatct cggcgattga
    34441 ctgttgtgaa tcaatttcaa ggtataaata acctttaagt aaaccttcgc taacaaataa
    34501 aaacaaatga tgatggtcgt ctaaactatc aatgatatct tgtggtgact gtgcctgata
    34561 tttaaatgta gttgtgtgta atttgctgaa agcacgataa aaccctttgt gataaggaat
    34621 gatatttggt tggtgcatat cttcttctaa tcttgtacgg gcttctatgt aatagtcagt
    34681 gaagttataa cttgcttgaa taactttcat taatggtttg tattgttgaa tgccttcttc
    34741 aaaagaaaag ttaaagacgg catcgtcagg ttggttcgaa gtcattgctt taaatagcgt
    34801 ttcaaacgta tcgacagata atacataatt gtcagccacg aaagggccta tgactttgaa
    34861 tttattttta tcgtatgcaa aagacaatat gattgctttg attgaatcgt tttctttaat
    34921 cgaaaataca ccaggtgatt caatcgcatc ggttattaat acatctattt cattgtactg
    34981 ttgaggaagt ttatataagt atgatgctga ttcataattc gaatgttcaa tgaacgattt
    35041 tatttgtgta atatcatata actgaactat gctcatgtca ttacctccgt actttttgtt
    35101 acttttatta tatagtattt caactgaaat gaaagttaat agtgatatta acatgttaca
    35161 atacatttaa cacatcattt gcaatcattg aatttaaatc aaagattagt ggaatagatg
    35221 aagcacgttc gaaataaaag aacgtatgag aaaggataat ttatgaaatc tttaatatta
    35281 gctgaaaaac catcagtcgc aagagatatt gctgatgctt tacaaataaa tcagaagcgt
    35341 aatggttact ttgaaaataa ccaatatatt gtcacgtggg cgttaggtca tctagtgaca
    35401 aatgcgacac ctgaacaata cgataaaaat ttaaaggaat ggcgattaga agaccttcca
    35461 attataccta aatatatgaa aactgttgtt attggtaaaa caagcaaaca atttaaaaca
    35521 gtaaaagcgt taattttaga taataaagtg aaagatatta ttattgcaac agatgctgga
    35581 cgagaaggtg aactagttgc aagattgatt ttggataaag ttggtaacaa aaagccaatc
    35641 cgtcgattat ggattagctc agttactaaa aaagctattc aacaaggttt taaaaattta
    35701 aaagacggtc gtcaatataa cgatttgtat tatgcagcgt tagcgagaag tgaggcagat
    35761 tggattgttg ggattaatgc aacgcgtgca ctaacaacaa agtatgatgc acagctatcc
    35821 ctgggacgtg ttcagacacc aacgattcaa ttagtaaata cacgacaaca agagattaat
    35881 cagttcaaac cacaacaata ctttacatta tcattaacgg taaaagggtt tgattttcag
    35941 ctagaatcaa atcagcgata taccaataaa gaaactttag aacagatggt taataatttg
    36001 aaaaatgtcg atggtaagat taaatctgtt gctactaaac ataagaagtc gtatccgcaa
    36061 tcactgtaca atttaacaga tttacaacaa gatatgtata gacgttataa aattggacct
    36121 aaagaaacat tgaatacact tcaaagctta tatgagagac ataaagtcgt aacctatcca
    36181 agaacagatt caaactattt aacaactgat atggtagata ctatgaaaga acgtattcag
    36241 gcgacgatgg caacaacata taaagaccaa gcacgcccat taatgtctaa aacattttca
    36301 tcaaaaatgt cgatatttaa taatcaaaaa gtatctgatc accatgcaat tattcctaca
    36361 gaagtgagac ctgtcatgtc agacttaagt aatagagaat taaagttata cgatatgatt
    36421 gtcgagcgtt ttttagaagc tttaatgcct ccgcacgagt atgacgcgat aactgtaact
    36481 ttagaggttg cagggcacac atttgttttg aaagagaatg taacaactgt tttaggtttt
    36541 aaatctatta gacaaggtga atctattaca gagatgcaac agcctttttc agaaggcgat
    36601 gaagtgaaga tttcaaaaac aaatattaga gaacatgaaa caacacctcc agaatatttt
    36661 aatgaaggtt cgttattaaa agcgatggag aaccctcaga actttattca attgaaggat
    36721 aaaaaatatg cgcaaacttt aaaacaaaca ggtggtatcg gcacagttgc aacaagggcc
    36781 gacattatcg ataaattatt taatatgaat gccattgaat caagagacgg taaaattaaa
    36841 gtaacgtcaa aaggtaaaca aatattagaa ttagcaccag aagaattaac gtcgccactt
    36901 ttaactgcac aatgggaaga aaaattactt ttaattgaac gtggtaaata tcaggcgaaa
    36961 acatttatta atgaaatgaa agattttacg aaagatgttg taaatgggat taaaaatagt
    37021 gatcgtaaat ataaacacga taatttaaca accacagaat gcccaacgtg tggtaaattc
    37081 atgattaaag ttaaaactaa aaatggtcag atgcttgtgt gccaagatcc atcttgtaag
    37141 acgaaaaaga atgtacagcg caaaacaaat gcaagatgtc caaactgtaa aaagaaatta
    37201 acgttgtttg gtaaagggaa agaagcggta tatcgttgtg tttgtggaca ttctgaaacg
    37261 caagcacata tggatcagcg tatgaagtct aaatcctctg gtaaagtatc tcgtaaagaa
    37321 atgaaaaagt atatgaataa aaatgaaggt ttagacaata atccgtttaa agatgcatta
    37381 aagaacttga atttatagat aaaatcgaac aaagttgaat cagaaaaacg aaaagttcgc
    37441 ttttggtatt gttttttatt aagaatgata ttaaactatt aaggtatttt aaaaaaagga
    37501 gcatccattc gtgaaaaact atttccagtt cgataaatat ggaacaaact ttaaaagaga
    37561 aatcttaggc ggtatcacaa ctttcttatc tatggcctat attttagcag ttaacccgca
    37621 agttttaagt ttagcaggtg ttaaaggcgt atcagaagat atgaaaatgg accaaggtgc
    37681 catttttgta gcgactgcat tagcagcatt tgtaggctcg ctattcatgg ggctaatagc
    37741 taaatatcca attgcgttag caccaggtat gggattaaat gcattctttg catttactgt
    37801 tgtgttaacc atgggtattc cttggcaagt tggtttaaca ggcgttttat tctcaggtat
    37861 attctttgcg atattaaccg tcacagggtt tagggaagtt attattaatg caattcctta
    37921 tcaaatgaag atggctgttt cagcaggtat aggattattc attacatttg taggtttaca
    37981 aagttcgggt attatagtta aaaatgaatc tacattagta acattgggac atttaactga
    38041 tccaccagta cttttggcga tattcggtat cgttattaca gttattttat atgcaattaa
    38101 attgccgggt tctatcttta tcggtatgat catcacagca atcgttggta tgttcactgg
    38161 attgattcaa atgccatctg gtattgtggg taagattcca agtattgaac caacgttcgg
    38221 tgctgcattt gaagcgttta aagacccaag tcaattatta acgatacagt tcttaattgt
    38281 tattttaacg ttcttattta ttgatttctt tgatacagct ggtacattag tagctgttgc
    38341 tactcaagca ggtattatga aagataataa attaccaaga gctggtagag cattgttctc
    38401 agattcttta gctacgattg taggatcagt ttttggaaca actacaacaa cttcttatat
    38461 cgaatcaact tcaggtgttg ctgtaggtgc tagaacagga tttgctagta tcgtaacagg
    38521 ttgttgtttc ctattagcgc tgttctttag tccgttaatc gctgttgtaa caagtgcagt
    38581 tacgacacca gccttagtgg tggtaggtgt attaatggct gcaaactttg ctgagataaa
    38641 ctggaaaagt tttgaagttg cagtgccagc cttcattaca atcattatga tgcctttatc
    38701 atattcaatt gcaactggta ttgcgtgtgg atttattttc tatccaatta caatgcttat
    38761 ctctaaaaaa cataaagaag tacatccgat aatgtatgta ttaatggtac tattcattct
    38821 gtacttcata tttgttcatg gttaaatcca gacacaaaaa acgactgcca atgattggta
    38881 gtcgtttttt tctttgaagt aaggataaaa taagaagcga aataacataa agcgatatta
    38941 tgaatgtctt taaatatccg atataaatat acttctttta aaattgttgg acgtgtttct
    39001 tctatattat atacctaatt aaaaaggttt taagaattat tctctatgtc catctttgta
    39061 ttgtgtgtaa atgacacgag catttttatt aggtatatag gttggcgctg ataatacaac
    39121 taggacaaat agaatcatta ggaaaaataa aatccaatga atcaaaattc ctaagaaggg
    39181 gataatagct aaaaaactac ctactatacc aaatattgga ataatcgcta taggtctaat
    39241 cgcattttgt ctattcacta ataatataat agtaagaatt agataaagta atgcattaaa
    39301 gagtaaaggc tgccatccaa aacttaaaat aaacgttgca cctaaaaaag gaatgccata
    39361 gaaaaactca caaagtatta aaaagatacc tataagtgct aaagataatt ttggtccatt
    39421 tttcatattc attcaccacc gttcttatga ctaattatat agaataattt tcaatgataa
    39481 taataaaatg attcaaggta cgaatttaca ataaagaagg aaataaaaaa tatctcgaaa
    39541 atagttgaac tgactaagta tataaagtaa aatataaaag tatgtgttag acagatagaa
    39601 aaattatgtg aaaagccttg atttatcgat gtttttcgtg taatataact aacgttggac
    39661 tttaagaaaa gcgatgaagc gaaaggttac tgacacaccc ggccgctttg ccatggcgct
    39721 gtgtaagata gttttcgtgg agaagtctat cactaaatgt agacgaataa ggagggaaaa
    39781 ttatggcaaa acaaaaaatc agaatcagat taaaagctta tgatcaccgc gtaattgatc
    39841 aatcagcaga gaagattgta gaaacagcga aacgttctgg tgcagatgtt tctggaccaa
    39901 ttccgttacc aactgagaaa tcagtttaca caatcatccg tgccgtgcat aagtataaag
    39961 attcacgtga acaattcgaa caacgtacac acaaacgttt aatcgatatt gtaaacccaa
    40021 caccaaaaac agttgacgct ttaatgggct taaacttacc atctggtgta gacatcgaaa
    40081 tcaaattata atagacaatt ttaggaggtg gactttcgat gaccaaagga atcttaggaa
    40141 gaaaaattgg gatgacacaa gtattcggag aaaacggtga attaatccct gtaacagtag
    40201 tagaagctaa agaaaatgtt gtattacaaa agaaaactgt agaagttgat ggatacaacg
    40261 caatccaagt tggatttgaa gacaaaaaag catacaaaaa agatgcaaaa tctaataaat
    40321 atgctaataa accagctgaa ggtcacgcta aaaaagctga cgcagcacct aagcgcttca
    40381 ttcgtgaatt ccgcaatgta gacgtggatg cttacgaagt aggtcaagaa gtctcagtag
    40441 atacttttgt agctggcgac gttattgacg taacaggcgt atcaaaaggt aaaggtttcc
    40501 aaggtgcaat taaacgccac ggacaatctc gtggacctat gtcacacggt tctcatttcc
    40561 acagagcacc aggttctgta ggtatggctt cagatgcttc tagagtattt aaaggccaaa
    40621 aaatgccagg acgtatgggt ggaaacactg taactgttca aaacttagaa gtagttcaag
    40681 ttgacacaga aaacaaagtt atcttagtaa aaggtaacgt acctggacct aaaaaaggtt
    40741 tagtagaaat cagaacttca attaaaaaag gtaataaata ataagaagtg aaaggaggaa
    40801 atgcataatg gctaattatg atgttttaaa attagacgga actaaatcag gttcaatcga
    40861 attaagcgat gcagtattcg gtattgagcc aaataatagc gttttattcg aagctattaa
    40921 tttacaacgt gcttcattac gtcaaggtac gcatgctgtt aagaatcgtt cagcagtaag
    40981 cggtggcgga cgtaaaccat ggaagcaaaa aggaacaggt cgtgctcgtc aaggtacaat
    41041 ccgtgctcca caatggcgtg gcggtggtat cgtattcgga ccaactccaa gaagttatgc
    41101 atacaaaatg cctaagaaaa tgcgtcgttt agctttacgc tcagcattat ctttcaaagc
    41161 tcaagagaat ggcttaactg tagttgacgc attcaacttc gaagctccaa aaactaaaga
    41221 attcaaaaat gtattatcta cattagaaca acctaaaaaa gtattagtag ttactgaaaa
    41281 cgaagatgta aatgttgaat tatcagcacg caacatccct ggcgttcaag tgacaactgc
    41341 tcaaggttta aatgttttag atatcactaa tgctgacagc ttagtaatta ctgaagctgc
    41401 tgctaaaaaa gttgaggagg tgctcggata atggaagcaa gagatattct taagcgcccc
    41461 gtaatcactg agaaatcttc tgaagcaatg gctgaagaca aatacacttt cgacgttgat
    41521 actcgtgtta acaaaacaca agtaaaaatg gcagttgaag aaatcttcaa cgtaaaagtt
    41581 gcaagtgtta atatcatgaa ttacaaacct aagaaaaaac gtatgggccg ttaccaaggc
    41641 tatacaaaca aaagaagaaa agcgattgta actcttaaag aaggatcaat cgacttattt
    41701 aactaataaa acaattacca ttaaggaggt aagcgacaat ggctattaaa aagtataagc
    41761 caataacaaa tggtcgtcgt aatatgactt cgttagattt cgcagaaatc acgaaaacta
    41821 cacctgaaaa gtcattatta aaaccgctac cgaaaaaagc gggacgtaac aaccaaggta
    41881 aattgactgt aagacaccat ggtggtggac acaaacgtca ataccgtgtt atcgatttca
    41941 aacgtaacaa agatggtatc aatgcaaaag ttgattctat tcaatatgat ccaaaccgct
    42001 cagcaaacat cgctttagtt gtatatgcag acggtgaaaa acgatatatc attgctccta
    42061 aaggattaga agtaggtcaa atcgttgaaa gtggtgctga agctgacatc aaagttggta
    42121 acgcattacc attacaaaac attccagttg gtacagtagt acacaacatc gagcttaaac
    42181 ctggtaaagg tggacaaatc gctcgttcag ctggtgcaag tgctcaagta cttggtaaag
    42241 aaggtaaata cgtattaatc agattaagat ctggtgaagt tcgtatgatc ttatctactt
    42301 gccgtgctac aatcggtcaa gttggtaacc tacaacacga attagttaac gttggtaaag
    42361 ccggacgttc aagatggaaa ggtatccgtc caacagttcg tggttctgta atgaacccta
    42421 acgatcaccc acacggtggt ggtgaaggtc gtgctcctat cggtagacca tctccaatgt
    42481 caccatgggg taaacctacg cttggtaaga aaactcgtcg tggtaaaaaa tcatcagaca
    42541 aacttatcgt tcgtggacgt aagaaaaaat aatataatca acttatttgg gtgtgcggct
    42601 taaagctgca cgcacataat aagaagggag gcgcccaaat ggctcgtagt attaaaaaag
    42661 gacctttcgt cgatgagcat ttaatgaaaa aagttgaagc tcaagaagga agcgaaaaga
    42721 aacaagtaat caaaacatgg tcacgtcgtt ctacaatttt ccctaatttc atcggacata
    42781 cttttgcagt atacgacgga cgtaaacacg tacctgtata tgtaactgaa gatatggtag
    42841 gtcataaatt aggtgagttt gctcctactc gtacattcaa aggacacgtt gcagacgaca
    42901 agaaaacaag aagataatat ctattaagta gaggaggaca tcctaatgga agcaaaagcg
    42961 gttgctagaa caataagaat cgcacctcgt aaagtaagac tagttcttga cttaatcaga
    43021 ggtaaaaatg ctgctgaagc tattgcaatt ttaaaattaa caaacaaagc ttcatcacca
    43081 gtaattgaaa aagtattaat gtccgcttta gctaatgctg aacataacta tgacatgaac
    43141 acagatgaat tagtagttaa agaagcatat gctaacgaag gaccaacatt aaaacgtttc
    43201 cgtccacgtg cgcaaggtcg tgcaagtgcg attaacaaac gtacaagcca cattacaatc
    43261 gtcgtaagtg acggtaaaga agaagctaaa gaagcttaat taacttttaa ggagggaata
    43321 ctgtgggtca aaaaattaat ccaatcggac ttcgtgttgg tattatccgt gattgggaag
    43381 ctaaatggta tgctgaaaaa gacttcgctt cacttttaca cgaagattta aaaatccgta
    43441 aatttattga taatgaatta aaagaagcat cagtttctca cgtagagatt gaacgtgctg
    43501 caaaccgtat caacattgca attcatactg gtaaacctgg tatggtaatt ggtaaaggcg
    43561 gttcagaaat cgaaaaatta cgcaacaaat taaatgcgtt aactgataaa aaagtacaca
    43621 tcaacgtaat tgaaatcaaa aaagttgatc ttgacgctcg tttagtagct gaaaacatcg
    43681 cacgtcaatt agaaaaccgt gcttcattcc gtcgtgtaca aaaacaagca atcactagag
    43741 ctatgaaact tggtgctaaa ggtatcaaaa ctcaagtatc tggtcgttta ggcggagctg
    43801 acatcgctcg tgctgaacaa tattcagaag gaactgttcc acttcatacg ttacgtgctg
    43861 acatcgatta tgcacacgct gaagctgaca ctacttacgg taaattaggc gttaaagtat
    43921 ggatttatcg tggagaagtt cttcctacta agaacactag tggaggagga aaataataat
    43981 gttactacca aaacgtgtaa aatatcgtcg tcaacatcgt cctaaaacaa ctggtcgttc
    44041 taaaggcggt aactacgtaa catttggtga gtttggttta caagctacaa caacgtcttg
    44101 gatcacatct cgtcaaatcg aatctgctcg tatagcaatg acacgttaca tgaaacgtgg
    44161 cgggaaagtt tggattaaaa tcttcccaca tacaccatat actaaaaaac ctttagaagt
    44221 acgtatgggt gctggtaaag gtgcggttga aggctggatc gcagttgtta aaccaggtag
    44281 aattttattc gaagttgctg gcgtttctga agaagttgcg cgtgaagcac tacgtttagc
    44341 aagtcacaaa cttccagtaa aaactaagtt tgtaaaacgt gaggaattgg gtggtgaaac
    44401 aaatgaaagc taaggaaatt agagacttaa ccacttcaga aatcgaagaa caaatcaaat
    44461 cttcaaaaga agagcttttt aacctacgct ttcagttagc tacaggtcaa ttagaagaaa
    44521 ctgcacgtat tcgtacagta agaaaaacga ttgcacgtct aaaaactgtt gctcgtgaaa
    44581 gagaaattga acaaagtaag gctaatcaat aattaagtaa gaggaggtta caaaagtgag
    44641 cgaaagaaac gatcgtaaag tttatgtagg taaagttgtt tcagacaaaa tggacaagac
    44701 tattacagta cttgttgaaa cttacaaaac acacaaatta tacggtaaac gagtaaaata
    44761 ctctaaaaaa tacaaaactc atgatgaaaa caattcagct aaattaggag acattgttaa
    44821 aattcaagaa actcgtcctt tatcagcaac aaaacgtttt cgtttagtag agattgttga
    44881 agagtcagta attatttaat acaagtttag agataaggga ggtttaacta atgatccaac
    44941 aagaaacacg cttgaaagta gcagacaact ctggtgctcg tgaagttctt acaatcaaag
    45001 tattaggtgg atctggtcgt aaaacagcaa acatcggcga tgttatcgta tgtactgtta
    45061 aaaatgcaac accaggtggc gttgttaaaa aaggtgacgt tgtcaaagct gtaatcgtac
    45121 gtactaagtc aggtgttcgt cgtaatgacg gttcatacat caaatttgat gaaaatgcat
    45181 gtgttatcat ccgtgatgac aaaggcccac gtggtactcg tatcttcgga cctgttgctc
    45241 gtgaattacg tgaaggtaac ttcatgaaaa tcgtatcatt agcaccagaa gtactttaat
    45301 taataacaac caatacaaat aaggaggtgc ccacatgcat atcaaaaaag gtgacaacgt
    45361 taaagttatc gcaggtaaag acaaaggtaa agaaggtaaa gtaattgcta ctctacctaa
    45421 aaaagaccgt gtcgttgtgg aaggtgttaa cattatgaaa aaacaccaaa aaccaactca
    45481 attaaatcct gaaggtggaa tcttagaaac agaggcagca atccatgttt ctaatgtaca
    45541 attattggac cctaaaacaa acgaaccaac tcgtgtaggt tacaaatttg ttgatggtaa
    45601 aaaagttcgt atcgctaaaa aatctggcga agaaattaaa tctaataatt aataacaagt
    45661 tgaaaggagg atcccacttt gaaccgttta aaagaaaagt ttaacactga agttactgaa
    45721 aacttaatga aaaaattcaa ttatagttca gtaatggaag taccaaaaat agataaaatc
    45781 gttgtgaaca tgggtgtagg tgacgcagta caaaattcta aagtattaga caatgctgtt
    45841 gaagaattag aattgatcac tggtcaaaaa ccattagtaa ctaaagctaa aaaatcaatc
    45901 gcgactttcc gtttacgtga aggtatgcca atcggtgcga aagtaacact tcgcggtgaa
    45961 agaatgtatg aattcttaga caaattaatt tcagtatcat taccacgtgt acgtgacttc
    46021 caaggtgttt ctaaaaaagc atttgacgga cgcggtaact acactttagg tgttaaagaa
    46081 caattaattt tcccagaaat cgactatgat aaagtaagta aagttagagg aatggatatt
    46141 gttatcgtaa cgactgctaa cactgatgaa gaagctcgtg aattgttagc taacttcggt
    46201 atgccattcc gtaaataatt atttaaagga ggctaattaa gtggctaaaa cttcaatggt
    46261 tgctaagcaa caaaaaaaac aaaaatatgc agttcgtgaa tacactcgtt gtgaacgttg
    46321 tggccgtcca cattctgtat atcgtaaatt taaattatgc cgtatttgtt tccgtgaatt
    46381 agcttacaaa ggccaaatcc ctggcgttcg taaagctagc tggtaataaa aaagagtctg
    46441 aaaggaggca acaatcaatg acaatgacag atccaatcgc agatatgctt actcgtgtaa
    46501 gaaacgcaaa catggtgcgt cacgagaagt tagaattacc tgcatcaaat attaaaaaag
    46561 aaattgctga aatcttaaag agtgaaggtt tcattaaaaa tgttgaatac gtagaagatg
    46621 ataaacaagg tgtacttcgt ttattcttaa aatatggtca aaacgatgag cgtgttatca
    46681 caggattaaa acgtatttca aaaccaggtt tacgtgttta tgcaaaagct agcgaaatgc
    46741 ctaaagtatt aaatggttta ggtattgcat tagtatcaac ttctgaaggt gtaatcactg
    46801 acaaagaagc aagaaaacgt aatgttggtg gagaaattat cgcatacgtt tggtaataaa
    46861 aaataaggag gtgccataac atgagtcgtg ttggtaagaa aattattgac atccctagtg
    46921 acgtaacagt aacttttgat ggaaatcatg taactgttaa aggtcctaaa ggtgaattat
    46981 caagaacttt aaatgaaaga atgacattca aacaagaaga aaacacaatt gaagttgtaa
    47041 gaccatctga ttctaaagaa gatagaacaa accatggtac aactcgtgct ttattaaaca
    47101 atatggtaca aggtgtttct caaggatacg taaaagtact tgaacttgtt ggtgtaggtt
    47161 accgtgctca aatgcaaggt aaagacttaa tccttaacgt tggttattct cacccagtag
    47221 aaattaaagc tgaagaaaac attactttct cagttgagaa aaacacagtc gttaaagttg
    47281 aaggtatttc aaaagaacaa gttggagcat tagcatctaa catccgttca gtaagacctc
    47341 cagagcctta caaaggtaaa ggtattcgtt accaaggtga atacgttcgc cgtaaagaag
    47401 gtaaaactgg taaataatag ataaactcta aagaaaggag tattgaaatg atcagtaaaa
    47461 ttgataaaaa taaagtgcgt ttaaaaagac atgctcgtgt tcgtactaac ttatcaggta
    47521 cagctgaaaa gccacgttta aacgtatatc gttcaaacaa gcatatctac gctcaaatta
    47581 ttgatgataa taaaggcgta acattagctc aagcttcttc aaaagacagc gacattgcta
    47641 ctacagcaac taaagttgaa ttagcaacta aagtcggtga agcaattgct aaaaaagctg
    47701 ctgacaaagg cattaaagaa atcgtatttg accgtggagg atatttatat cacggacgtg
    47761 ttaaagcatt agctgaagca gcaagagaaa gcggattaga attttaattt aaaggaggga
    47821 caaatacatg gctcgtagag aagaagagac gaaagaattt gaagaacgcg ttgttacaat
    47881 caaccgtgta gcaaaagttg taaaaggtgg tcgtcgtttc cgtttcactg cattagttgt
    47941 agttggagac aaaaatggtc gtgtaggttt cggtactggt aaagctcaag aggtaccaga
    48001 agcaatcaaa aaagctgttg aagcagctaa aaaagattta gtagttgttc cacgtgttga
    48061 aggtacaact ccacacacaa ttactggccg ttacggttca ggaagcgtat ttatgaaacc
    48121 ggctgcacct ggtacaggag ttatcgctgg tggtcctgtt cgtgccgtac ttgaattagc
    48181 aggtatcact gatatcttaa gtaaatcatt aggatcaaac acaccaatca acatggttcg
    48241 tgctacaatc gatggtttac aaaaccttaa aaatgctgaa gatgttgcga aattacgtgg
    48301 caaaacagta gaagaattat acaattaagg agggaaaact agttatggct aaattacaaa
    48361 ttaccctcac tcgtagtgtt attggtcgtc ctgaaacaca acgtaaaact gttgaagctt
    48421 taggtcttaa aaagactaac agttcagtag ttgttgaaga taaccctgct attcgtgggc
    48481 aaatcaacaa agttaagcac ttagtaacag tagaagaaaa ataatcaagg aggtgccgaa
    48541 atgaaattac atgagttaaa accggcagaa ggttcacgta aagaacgcaa tcgtgttgga
    48601 cgtggtgttg cgacaggtaa cggtaaaaca agtggtcgcg gacacaaagg tcaaaaagct
    48661 cgttcaggcg gtggtgtaag accaggattt gaaggtggtc aattaccatt attccgtcgt
    48721 ttaccaaaac gtggttttac taacataaat cgtaaagaat atgctattgt taacttagac
    48781 caacttaata aatttgaaga tggtactgaa gtaactccag ctttattagt agaatctggt
    48841 gttgttaaga atgaaaaatc tggtatcaaa atactaggta atggttcact tgataagaaa
    48901 ttgacagtga aagctcataa attctcagct tcagcagcag aagctattga tgctaaaggt
    48961 ggagcacacg aggtgatcta atgattcaaa cccttgtgaa cttctttaga acaaaagagg
    49021 ttcgtaacaa gattttcttc acactagcaa tgttagtaat ttttaaaata gggacttata
    49081 taccagctcc aggagtaaat cctgcagctt ttgataatcc ccaaggttct caaggtgcca
    49141 ctgagttatt aaatactttt ggtggcggag ccttgaaacg attttctatt tttgcaatgg
    49201 gtattgtacc ctacatcact gcatcaatcg taatgcaatt attacaaatg gatattgtcc
    49261 ctaaattctc agaatgggca aaacaaggtg aagtaggtag aagaaagtta aataacgtta
    49321 ctcgttattt agcaatttct ttagcattta tccaatctat aggtatggca ttccaattta
    49381 ataattatct caaaggtgcg ctgattatca atcagtcaat tatgagttat ttattaatag
    49441 cactagtttt gacagcagga actgctttct taatatggct tggtgatcaa atcactcagt
    49501 tcggtgttgg taatggtatt tctattatca tatttgcggg tattttatca acgttgccag
    49561 ccagtctaat tcaatttgga caaactgcgt tcgttgggca agaagataca tcattagcat
    49621 ggttgaaagt attaggactt ttagtgtcac tgatactatt aactgtaggt gctatttatg
    49681 tacttgaagc cgttcgtaaa attccgattc aatatgctaa gaaacaaact gctcaaagat
    49741 taggttcaca ggcaacttat ctacctttaa aagtgaactc agccggtgtt attcctgtaa
    49801 tctttgcaat ggcgttcttc ttactgccta gaacattaac gttgttctat ccagataaag
    49861 aatgggcaca aaacattgcg aatgctgcaa atccttcaag taatgttggt atggttgtat
    49921 atattgtact aattatacta ttcacatact tctatgcatt tgtacaagtt aatccggaaa
    49981 aaatggctga taaccttaag aaacaaggta gctatgttcc aggtattaga cctggtgaac
    50041 aaaccaaaaa atatattact aaagttttat atcgtttaac ttttgtaggt tctatcttct
    50101 tagccgttat ttcaattcta ccgatattag caactaagtt tatgggatta ccacaatcaa
    50161 ttcagattgg tggtacaagt ttacttatcg ttattggtgt agcgattgaa acaatgaaat
    50221 ctttagaagc tcaagtatct caaaaagaat ataaaggctt tggtggtaga taattttagg
    50281 agggcactta tgaatatcat tttgatgggt ttacctggcg caggtaaagg aactcaagca
    50341 agtgaaattg tcaagaaatt cccaataccc cacatttcaa ctggtgacat gttcagaaaa
    50401 gctataaaag aagaaactga attaggtaaa gaagctaagt cttatatgga ccgtggcgaa
    50461 ttagttcctg atgaagtgac tgtaggtatc gttaaggaaa gaatttctga agacgatgca
    50521 aaaaaaggct ttttattaga tggcttccca agaacaatcg agcaagctga ggcattaaat
    50581 aatattatgt ctgagcttga cagaaacatt gatgctgtca tcaatatcga agttccggaa
    50641 gaagaattaa tgaaccgtct tacaggtcgt cgaatctgtg agtcatgtgg tacaacgtat
    50701 catcttgtat ttaatcctcc gaaggtcgaa ggtatttgtg atatcgatgg tggtaaattg
    50761 tatcaacgag aagatgataa tcctgaaacg gtagctaatc gtttgagtgt taatattaaa
    50821 caatctaagc ctattttaga tttctatgat caaaaaggtg tattgaaaaa tattgatggt
    50881 tcaaaagata ttagcgacgt taccaaagat gtcattgata ttttagatca tttgtaatag
    50941 atcaactttt agcggtctat tttaagtgag gcatgattta atcatattag atagatgaga
    51001 gtggcaatta acgataatta attcccgtaa tttttgttta acttataaaa ttaatcactg
    51061 atagattcgt aacaataaag ttgtctattg acgattacct tactattgca aaaaggggga
    51121 agttaatcaa tggctaaaca agatgtaatt gaattagaag gtactgtatt agatacttta
    51181 ccgaacgcaa tgtttaaagt agaattagaa aatggtcatg agattttagc tcacgtaagt
    51241 ggtaaaatca gaatgaatta cattcgtatt ctacctggcg acaaagtaac tgttgagatg
    51301 tctccgtacg atttaacacg cggaagaatt acttatcgtt ataaataatc gtcactccat
    51361 aatataggga ggtataaaaa tgaaagtaag accatcagta aaacctattt gcgaaaaatg
    51421 taaagtcatt aaacgtaaag gtaaagtaat ggtaatttgt gaaaatccaa aacacaaaca
    51481 aagacaaggt taataaaaga gaggtgtaaa ttaatatggc acgtattgca ggagtagata
    51541 ttccacgtga aaaacgcgta gttatctcat taacttatat atacggtatc ggtacgtcaa
    51601 ctgctcaaaa aattcttgaa gaagctaacg tatcagctga tactcgtgtg aaagatttaa
    51661 ctgatgacga attaggtcgc atccgtgaag ttgtagacgg ttataaagtc gaaggtgact
    51721 tacgtcgtga aactaactta aatatcaaac gtttaatgga aatttcatca taccgtggta
    51781 tccgtcaccg tcgtggttta ccagttcgtg gtcaaaaaac gaaaaacaac gcgcgtactc
    51841 gtaaaggacc agttaaaacg gtagctaaca agaaaaaata ataggtaaag gaggcaaatt
    51901 ttaaatggca cgtaaacaag tatctcgtaa acgtagagtg aaaaagaata ttgaaaatgg
    51961 tgtagcacac atccgttcaa cattcaacaa cactattgta actatcactg atgagttcgg
    52021 taatgcttta tcatggtcat cagctggtgc attaggattc aaaggatcta aaaaatcaac
    52081 accatttgca gcacaaatgg cttctgaaac tgcatctaaa tcagctatgg agcatggttt
    52141 aaaaacagtt gaagtaacag ttaaaggacc tggtccaggt cgtgaatcag ctattcgtgc
    52201 attacaatct gcaggtttag aagtaactgc gatcagagac gttactccag tacctcataa
    52261 cggttgtcgt ccaccaaaac gtcgtcgtgt ataatttatg atggtattgt tacaggtcac
    52321 tgagcaaaca ttttaaatta agtcgacgta tataaggagg atatttaaat gatagaaatc
    52381 gaaaaaccta gaattgagac aattgaaatt agtgaagatg ctaaattcgg taagttcgtt
    52441 gttgaaccac tagaacgtgg ctacggtact acactaggaa actccttacg tcgtatccta
    52501 ctatcttcat taccaggtgc agccgttaag tatattgaaa ttgagggagt tttacatgaa
    52561 ttctcagcag tagacaatgt agttgaagat gtttctacaa ttattatgaa cattaaacaa
    52621 ttagcattga aaatttactc tgaagaagat aaaactttag aaattgatgt acgtgatgaa
    52681 ggcgaagtaa cagcaagcga cattacacat gatagtgatg ttgaaatttt aaacccagag
    52741 cttaaaattg caacagtatc taaaggtggt cacttaaaaa ttcgtctagt tgctaacaag
    52801 ggtagaggtt acgcattagc agaacaaaat aatactagtg atttaccaat tggtgtaacc
    52861 cctgttgatt cattgtattc acctgttgaa cgtgtgaact atactgttga aaatacacgt
    52921 gtaggtcaaa gcagtgattt tgataaatta acattggatg tttggactaa tggttcaatc
    52981 acaccacaag aatcagtttc attagcagca aaaataatga ctgaacactt gaatatcttc
    53041 gttggtctta ctgatgaagc gcaaaacgct gaaatcatga ttgaaaaaga agaagatcaa
    53101 aaagaaaaag tattagaaat gtctattgaa gaattagact tatctgtacg ttcatataac
    53161 tgcttaaaac gcgcaggaat caattctgtt caagagttag ctgacaaatc tgaagctgac
    53221 atgatgaaag tgcgtaattt aggtcgtaaa tctttagaag aagttaaata caaattagaa
    53281 gatttaggat taggattaag aaaagaagat tgataaagga ggttaactca tgggttacag
    53341 aaaattaggt cgtacttctg atcaacgtaa agctatgtta cgtgacttag ctacatcact
    53401 tattattagt gaacgtattg aaactacaga agctcgtgca aaagaagttc gcagtgttgt
    53461 tgagaaatta atcactttag gtaaaaaagg agatttagct tctcgtcgta atgcagctaa
    53521 aactttacgt aatgttgaaa tcttaaacga agatgaaact acacaaactg cacttcaaaa
    53581 attatttggt gaaatcgcag agcgttacac agaacgtcaa ggtggttaca ctcgtatcct
    53641 taaacaaggc cctcgtcgcg gtgacggtgc tgaatcagta attatcgaat tagtataaga
    53701 atttatataa atacactaac tacttttata taaagcaaat gagtgcaacg ataatgtttg
    53761 ccacatacaa atattgtcta gctcagagta ccccatcaac taaataaaga tttaaagcgt
    53821 gaactcaaca aacttatgat ggggtgcgcg cttttttatt gaataaaata tcggacttat
    53881 gaacagaaat gtttttaagt ccgatttctt tatatttact tttttgagtg cgaatctaaa
    53941 aatatgatga agctagtttg atcaatttca ctagaactga atgaagtgaa agtattatcg
    54001 ttgaatcaaa cgcgaccacc cctatattag taaatcattt attttattaa cgtttataca
    54061 taattgttag agaggaataa aagtccttat cattagttgt taaacaacac tttaaagact
    54121 agaaatgagt cagaaaatac tagtaatata tagagaaccc agtcaatgtc atatacagcg
    54181 atttataaat attgtaaaat atgatagata cacgaatcgt aaaggagaga gacaagtgga
    54241 ggataagaat tcagttattg tatttaaaaa tgtttcattt caatatcaaa gtgatgcatc
    54301 cttcacattg aaagatgttt cttttaatat acctaaaggt cagtggacat ctattgttgg
    54361 tcataacggt tctggaaaat ctacaattgc caagttaatg attggcatag agaaagttaa
    54421 atctggagaa attttttata ataatcaagc tataactgat gataattttg aaaagttaag
    54481 aaaagacata ggaattgtat ttcagaatcc ggataatcaa tttgttggtt caattgtaaa
    54541 atacgatgtg gcatttggac tcgaaaatca tgcggttcca tatgacgaaa tgcatagaag
    54601 agtcagcgaa gcacttaaac aagttgatat gttagaacgt gcagattatg aacctaatgc
    54661 attatcgggg ggacagaagc agcgtgtggc tatagcaagt gtattagcac ttaacccctc
    54721 tgtcattata ttagatgagg cgacttctat gttagatcct gatgcacgtc aaaatttatt
    54781 ggatttagtg agaaaagtta aatcagaaca taatattaca atcatttcta ttacgcatga
    54841 tttatctgag gcgatggaag cagatcatgt tatcgttatg aataaaggga ctgtctataa
    54901 agaaggcaca gcgactgaaa ttttcgacaa tgcagaagag ttaacaagaa taggtctaga
    54961 tttgccattc ccaatcaaaa taaatcaaat gctgggacac caaacatcat tcttaactta
    55021 tgaagggctg gtggatcaac tatgactata cggtttgaca atgtaagtta tacctatcaa
    55081 aaagggacac catatcagca tcaagctatt catgatgtta atacagaatt tgaacaaggt
    55141 aaatattacg ccatcgttgg acaaacgggt agtggtaaat caacgttgat acaaaatatt
    55201 aatgcgctgt taaagccgac tactgggaca gttacagttg atgacattac tattacacat
    55261 aagaccaaag ataaatatat tagacctgta agaaaaagaa ttggaatggt atttcaattt
    55321 cccgaatctc aattatttga ggacacagta gagcgtgaaa tgatatttgg acctaaaaac
    55381 tttaaaatga atttagatga agccaaaaac tatgcccatc gtctgttgat ggatttaggc
    55441 ttttcaagag atgtaatgtc acaatcacca tttcaaatgt caggtggaca aatgcgtaaa
    55501 atagcgattg tatcgatatt ggcaatgaat cctgatatta tcgtggttga tgaacctaca
    55561 gcaggacttg atccacaaag taaacgacaa gtaatgagat tactaaagtc actacaaaca
    55621 gatgaaaata aggcaattat cctaatttca catgatatga atgaagtcgc gcgttatgca
    55681 gatgaagtca tcgttatgaa agaaggtagt atagtgtcgc aaacatcacc aaaagagctc
    55741 ttcaaagata aaaaaaaatt ggcggattgg catattggtt tgccagaaat tgttcagtta
    55801 caatatgact ttgaacaaaa atatcaaaca aaattaaaag atattgcctt aactgaagaa
    55861 gcatttgtaa gcttgtataa ggagtggcaa catgaaaaat aaattgatta tagggcgata
    55921 tttaccaatt aattcttttg ttcatcatct tgatccaaga gcaaagttaa tgtttgtttt
    55981 tctttttatt atattaatat ttttctgtca ttcaccatta acatatcttt gggtgtttgc
    56041 acttatctta ttcattatga gattagcaaa aattcaatta tggttcctaa tcaaaggttt
    56101 aacaccaata tttttctttt taatcttcac attaatgatg catatatttt taactaaagg
    56161 tggatatgta ttagttgaat ggcatggcat cacgattgaa actaatggta ttttagaagg
    56221 gctatatata tcactgcgtt taattgggat tgtaatgatt gcaacaatta tgacactatc
    56281 tacaagtcca attgatttaa cagatgcgtt tgaaagatta cttgcaccac taaagatgtt
    56341 taaattacca gttcatcaat taagtatgat aatgtccatt gcgttacgat tcatcccgac
    56401 gttaatggat gagttagata aaatcatttt ggcgcaaaag tcgcgtggtt ctgaaataag
    56461 ttcagggaac attgcaacac gtattaaatc atttattcca ttactagtgc cactattcat
    56521 ctcagctttc caacgcgccg aagaattagc ggtcgcaatg gaagttaggg gttatgatgc
    56581 caatgttaag agaacgagct acagacagct taaatggcaa ttgagagata cgatatcttt
    56641 aaccatgatt atcccaattg caattatttt attcgtatta aaatattcag gagtgtaaat
    56701 tcatgcgtat attagtagaa attgcgtatc aaggaaataa ttttctaggt tttcaaattc
    56761 aacaaaatgg tcgaacggta cagcaacaat ttgaaaagct attacaacgc atgcataaaa
    56821 gacatgtgag aatccatcct tcaagtagga ctgatagagg tgtacatgcg atacagcaat
    56881 actttcattt cgacacagag ctgaatatac ctatgtcaca atggcaatat gcaatgaacc
    56941 gaacgttacc tgatgatatt tatgtcaaca atgtagttac agtcgatgac gattttcatt
    57001 gtcgttatga ttgtgtaggt aaacgttatc gctacaaagt atatcaagca caacaccgcg
    57061 atccatttca aagtggtttg aaaacattta ttcctgaaac gttggattta ggtaaaatga
    57121 acagagcggc gcaacaattc ataggtacac atgattttac gggattttgt tcgcaaaaaa
    57181 ctgaagtaga aagcaaagtt agaacattat accaaagtga aatcgtaaag acagatgatg
    57241 gctttgatta tattgtcact ggatctggat ttttatataa catggtgcgt gtcttagttg
    57301 catttttaat agaagttgga aaagggcgac atgaagtatc tgatgtacca aaacttcttg
    57361 aatcaaagaa tcgtaagaat gtacctttca ctgcaccggc tgaaggatta tatttagaga
    57421 aaatttattt agatgaaaat gagttattaa aagactttgg taacgatatc aaaatacatc
    57481 gtaaaaaatc attacaaaat gactgattgc cattgacaaa aagcttgtga aattataaga
    57541 ttattaacgg tattgtttta tatccacccc acgataagcc ccggaaactt attgtgttac
    57601 aagatatata agcagaaacg aacaacagtt aacaaaataa atgaaattaa acgttttaaa
    57661 aatgaaacaa atgaaatcat ctattaggtt atgaaactgt ttatagcttg aatagaagca
    57721 tttatttttt aggaggacaa ttattatgcg tcaaacattt atggcaaatg aatcaaacat
    57781 tgagcgcaaa tggtatgtta tcgatgctga aggccaaaca ttaggtcgtt tatcatcaga
    57841 agtagcatct atcttacgcg gtaaaaataa agtaacttac acaccacacg ttgatactgg
    57901 tgattatgta atcgttatta atgcatcaaa aatcgaattt actggtaaca aagaaactga
    57961 caaagtttac taccgtcact caaatcaccc aggtggtatc aaatcaatca ctgctggtga
    58021 attaagaaga actaacccag aacgtttaat tgaaaactca attaaaggta tgttaccaag
    58081 cactcgttta ggcgaaaaac aaggtaaaaa attatttgta tatggtggcg ctgaacatcc
    58141 acacgctgca caacaaccag aaaactacga attacgtggt taattagaag gaggaaatga
    58201 ctttggcaca agttgaatat agaggcacag gccgtcgtaa aaactcagta gcacgtgtac
    58261 gtttagtacc aggtgaaggt aacatcacag ttaataaccg tgacgtacgc gaatacttac
    58321 cattcgaatc attaatttta gacttaaacc aaccatttga tgtaactgaa actaaaggta
    58381 actatgatgt tttagttaac gttcatggtg gtggtttcac tggacaagct caagctatcc
    58441 gtcacggaat cgctcgtgca ttattagaag cagatcctga atacagaggt tctttaaaac
    58501 gcgctggatt acttactcgt gacccacgta tgaaagaacg taaaaaacca ggtcttaaag
    58561 cagctcgtcg ttcacctcaa ttctcaaaac gttaattgtc ggacgatata tacaaaacac
    58621 ctcgatatta tgtcgaggtg tttttttgcg tttttgcggc gaatatggaa tgtgtagaat
    58681 ataaatgaat ttttaccttc ccaccataaa agatgaagaa ccatgaaatg tggagaacaa
    58741 taaatagttg atattctgtt atttttttgg aagtgaagtg atttgaatac tttactctaa
    58801 acgataaaag gttaaaaaaa caacaaaaag aaaatattac tgtcataatt ggaaatacac
    58861 cgtattctgc tggtcaatct agtgtaaata atgataataa aaaaatgaat atgaaaaatt
    58921 aaatgcaaaa atagcaaata cctatgcaaa tttttcaaca gcaaatttaa aaaaatcatt
    58981 atatgattcg tatatacagg cttttcgatg ggcaactgat agagttggaa aaaaggtatt
    59041 ataggatttg taacaaatgg atcatttatt gatagtcaaa gcactgatgg atttagaaaa
    59101 gttttatacg atgaatttaa ctatctgtac ataattaatt tgagaggcga ccaacgaaca
    59161 cagggtgaaa aatcaagaaa agaaggtgga aaaatatttg gatcaggaag tagagcaccg
    59221 atagctatat ctattttagt taaagatgga agttacaatc acgatatcta ttataatgac
    59281 attggtgaat atttaactag agagcaaaaa ttagatactt taatgaaaca tcaatcaatt
    59341 gtgaatttga aaagtttgaa tgttttaccc gataaaaaca atgattggat taatcaacga
    59401 gacataaact atgagaatta tttaccaatg tatgattcca aggatattga aaattctata
    59461 tatttagatc aatttaatgg agtaaattca gcaagagata attgggtaac aaatttttcg
    59521 aatgaaaaag cattagtaaa tgcaaaatta cttgtagata actataattc tgaaatagat
    59581 agattaattg atatcttaga ttcaagagaa cgtataaatt tagtgaacaa agatgaaaca
    59641 tttattagtt ggacacgagg acttacccaa aaattttcta aaggaaaaaa tatttcaatt
    59701 aatccagaga gaattgttaa atttatgcat agaccattta caaaaaaatg gatagtgtac
    59761 gacaaaaata ttatggaaat gccaagtaga tattataata taatggagaa tactggacag
    59821 gtgatttata tacaaggaca gggaatgaat aaagaattct cggctatgat cacggacata
    59881 ttacctaatt tccaatttat tggtaatggc aaaggctttg ctacatacaa aggtaaagat
    59941 agtttgaggc ttgtagataa tatttctaat tcttttaaaa agaaaataaa tcttaattca
    60001 gaagagatag tatattatat atatgcaatt ttgcatcata aatattatgt gaacaaatat
    60061 tcaagtgatt tgagtaaagg ttttccgcga ataccgatat taaaggatgt atatggattt
    60121 gttgaaattg gtagggagtt agtggaatta catttaaatt atgaaaaaca actcaattgg
    60181 gatggtgtag aaataattta taataatatg aatccgaatt ataaagttga aaaatgaagc
    60241 accctaaaaa gggcgtgttg gatacagtaa tatacaatac cgatattaca gttactaaca
    60301 ttccaaataa agtatataat tatattgtga atggcaagcc tgctattgaa tggattatag
    60361 atcagtacaa cgtaagcatc gacaagaaat ctggaatatt agatgatcct aatgaattta
    60421 gtgaagatcc gaattacatt ttaaatctgc ttttatcagt tattactgta agtatgaaaa
    60481 ctcttggatt aatagataaa ttaccagatt ttgaagtatt agattaagga gaggatatat
    60541 tgacaaatac tatcaaaaaa ataatttatt ttgatgaact atcagcaatg gatttattac
    60601 agatagagaa gaaaggtaac cttactaaaa cagttcagtt gatgcatgat gaaaaaggta
    60661 gtgcaagtgg tgaatctaaa gtgaacatgg aatttggtaa gcaaagcgct gtaaaacaaa
    60721 tttttgattc tttacttggg atgtcagcga gtgcttcggc tagtgcaggt ataaatggaa
    60781 atataactgg agagcgagta gccaaaacaa ttttagagaa tacattaata tatgattttt
    60841 tggatacagt agagtttaga aagaaaaaaa ctattattga tatttcagag gggtttaaac
    60901 taaatattca acaagatacg atgacatatt tcgcgactat tacccccata gcaagtatgt
    60961 ttgaaggatc taaacatcta gacgaggagg agctatcatt tgatgtttca aaaatgaatg
    61021 aagcgattag aagtattaaa ggctattttg aattaatagg aacaagaggg aatgaaaaca
    61081 gagtttttag atttaatata gaatctttta aaaataacta tagaatacaa gatttaaaat
    61141 ttatgaattt aacactctac tcagtacttg ttggtgaaat aagcaaagaa aatctaaatt
    61201 acgataaaga gtttgatcta aatcaaggtg ccaaacctag tggaattgga aagtttaagg
    61261 gaattagatc agaagcaact aaagaagata aatatcaagt gtatgatgtt attcttgcag
    61321 gagtgaaata aatggaaatt catgtttata cgggaccgct ttgtggattt aatgaatata
    61381 ttaattctga agttaaagaa tatcaccgct tcatggaatt aatcagagaa tacaatatca
    61441 tagtgcgagc aagtgataca tttggaactc atgatgttga tggtattttt gatactgaga
    61501 tagaaaatct tgtgatattt tcaaacgact ttgcttctgt aacaactcat gttattacta
    61561 attttataaa tatcgttact ttaggtagaa atattaagaa aatctatatt cagaatcctc
    61621 caaaaagagt atttcaatca ctaaatagtt ttcacgattc tgaaataata gaaataaact
    61681 atgaatatac aacattaaaa aaagatgatg taataaattt ttataaagag cagatggctg
    61741 gttctaaaat aataggtcaa acagacgcta aaaaacaaat gagcatagat ttatataaac
    61801 atacttcctt agaaaataat aaaccgaccg taatgcttta ctatggtcca tcaggtgttg
    61861 gaaaaacaga attagccaaa gaaatagcaa aaaaatataa aggtaatatc actagaatac
    61921 aattttctat gatgcagaat gatgaatctt ttaattatat ctttggagat gaacatgcca
    61981 aagtatcatt gtcaaaagat ttagtagatc gtgaaactaa tgttgtttta attgatgaat
    62041 ttgataaggt attaccaatc tattacaatg ttttctatca aatgtttgat gaaggtatta
    62101 ttgaagatgc caattataaa gtagacgttt caaattgtat tttcattcta acatctaatt
    62161 ttaatagtat tgaagaagca atagttaata ttggagaacc ggtattttct agaatagatt
    62221 cctgtattaa atttttatat ttaacacagg aagaaaagca gaaagtaata gaaaataaat
    62281 taaatgaaat actatcatct ctaaatgaaa aagaaaaaag aatagttacc gcatataact
    62341 tgttagagaa atataaagag acagaaatag atatcgataa cattaggtat ttaaagaaaa
    62401 taataacaaa tgatat
//
LOCUS       DALAGF010000003        61207 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.003, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000003 DALAGF010000000
VERSION     DALAGF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 61207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61207
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.003"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(552..653)
                     /locus_tag="O2692_000161"
     CDS             complement(552..653)
                     /locus_tag="O2692_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297813.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNSVVIGGKGK"
     gene            738..2126
                     /locus_tag="O2692_000162"
     CDS             738..2126
                     /locus_tag="O2692_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCZ4297814.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLIYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FDSNDNAALTSH"
     gene            complement(2207..2962)
                     /locus_tag="O2692_000163"
     CDS             complement(2207..2962)
                     /locus_tag="O2692_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HCZ4297815.1"
                     /translation="MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            3454..4023
                     /gene="ahpC"
                     /locus_tag="O2692_000164"
     CDS             3454..4023
                     /gene="ahpC"
                     /locus_tag="O2692_000164"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCZ4297816.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            4039..5562
                     /gene="ahpF"
                     /locus_tag="O2692_000165"
     CDS             4039..5562
                     /gene="ahpF"
                     /locus_tag="O2692_000165"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCZ4297817.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKDLLTEIT
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKKLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMNTGEEHLLNLDGIFVQIGLLPNTSWLNDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            5692..5934
                     /locus_tag="O2692_000166"
                     /pseudo
     CDS             5692..5934
                     /locus_tag="O2692_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6007..6633
                     /locus_tag="O2692_000167"
     CDS             6007..6633
                     /locus_tag="O2692_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HCZ4297818.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            6888..7271
                     /locus_tag="O2692_000168"
     CDS             6888..7271
                     /locus_tag="O2692_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297819.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFTSTIITLAIGGKSVEKNDSP"
     gene            complement(7337..7918)
                     /locus_tag="O2692_000169"
     CDS             complement(7337..7918)
                     /locus_tag="O2692_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCZ4297820.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYDKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSRAEAVDLKVENCKPYIIE"
     gene            8096..8305
                     /locus_tag="O2692_000170"
     CDS             8096..8305
                     /locus_tag="O2692_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297821.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILAFLFPKFPHINLLLF
                     NPILSTFSNIIIHIDHFHANKKDDN"
     gene            complement(8344..8595)
                     /locus_tag="O2692_000171"
     CDS             complement(8344..8595)
                     /locus_tag="O2692_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCZ4297822.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
     gene            8895..9158
                     /locus_tag="O2692_000172"
     CDS             8895..9158
                     /locus_tag="O2692_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4297823.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            complement(9296..9868)
                     /locus_tag="O2692_000173"
     CDS             complement(9296..9868)
                     /locus_tag="O2692_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCZ4297824.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTMNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(10049..10417)
                     /locus_tag="O2692_000174"
     CDS             complement(10049..10417)
                     /locus_tag="O2692_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCZ4297825.1"
                     /translation="MKTILKTITYLVLTIIGAYAALFILKTIDSHGITDQFNPLVKKD
                     DSYVKTTEVSTRMDDQLRSYSQSVFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            complement(10786..11397)
                     /locus_tag="O2692_000175"
     CDS             complement(10786..11397)
                     /locus_tag="O2692_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCZ4297826.1"
                     /translation="MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQ
                     DKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYTVAGRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
     gene            11597..12304
                     /locus_tag="O2692_000176"
                     /pseudo
     CDS             11597..12304
                     /locus_tag="O2692_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505297.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            12343..13281
                     /locus_tag="O2692_000177"
     CDS             12343..13281
                     /locus_tag="O2692_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCZ4297827.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKYLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRLPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(13547..13789)
                     /gene="rpsR"
                     /locus_tag="O2692_000178"
     CDS             complement(13547..13789)
                     /gene="rpsR"
                     /locus_tag="O2692_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCZ4297828.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(13841..14344)
                     /gene="ssb"
                     /locus_tag="O2692_000179"
     CDS             complement(13841..14344)
                     /gene="ssb"
                     /locus_tag="O2692_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCZ4297829.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            complement(14365..14661)
                     /locus_tag="O2692_000180"
     CDS             complement(14365..14661)
                     /locus_tag="O2692_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCZ4297830.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            14905..15096
                     /locus_tag="O2692_000181"
     CDS             14905..15096
                     /locus_tag="O2692_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297831.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            complement(15182..16279)
                     /gene="ychF"
                     /locus_tag="O2692_000182"
     CDS             complement(15182..16279)
                     /gene="ychF"
                     /locus_tag="O2692_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCZ4297832.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(16291..16494)
                     /locus_tag="O2692_000183"
     CDS             complement(16291..16494)
                     /locus_tag="O2692_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCZ4297833.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIESHDDTQR"
     gene            complement(16524..17405)
                     /locus_tag="O2692_000184"
     CDS             complement(16524..17405)
                     /locus_tag="O2692_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCZ4297834.1"
                     /translation="MNQVMNIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVEENIDNVDKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAIEDTRVILRISAETIPGEGFAGARIIRKEVQKMFLQEGI
                     KTPQPIMTAYNHSENGV"
     gene            complement(17562..18407)
                     /locus_tag="O2692_000185"
     CDS             complement(17562..18407)
                     /locus_tag="O2692_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCZ4297835.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFVRIIEQLN
                     RRYGK"
     misc_binding    18636..19005
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            19071..20174
                     /locus_tag="O2692_000186"
     CDS             19071..20174
                     /locus_tag="O2692_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCZ4297836.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     gene            20171..21331
                     /locus_tag="O2692_000187"
     CDS             20171..21331
                     /locus_tag="O2692_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HCZ4297837.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISD
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            21300..23141
                     /locus_tag="O2692_000188"
     CDS             21300..23141
                     /locus_tag="O2692_000188"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HCZ4297838.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAFLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLYGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            23138..25366
                     /gene="metE"
                     /locus_tag="O2692_000189"
     CDS             23138..25366
                     /gene="metE"
                     /locus_tag="O2692_000189"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCZ4297839.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            25409..26161
                     /locus_tag="O2692_000190"
     CDS             25409..26161
                     /locus_tag="O2692_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HCZ4297840.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(26244..27425)
                     /locus_tag="O2692_000191"
     CDS             complement(26244..27425)
                     /locus_tag="O2692_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCZ4297841.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            27964..29097
                     /locus_tag="O2692_000192"
     CDS             27964..29097
                     /locus_tag="O2692_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HCZ4297842.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLTSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISYSLGIL
                     VMSYIVSLLFKGHDHVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            complement(29464..30093)
                     /locus_tag="O2692_000193"
     CDS             complement(29464..30093)
                     /locus_tag="O2692_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HCZ4297843.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMICASILLLISFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIAYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(30093..30935)
                     /locus_tag="O2692_000194"
     CDS             complement(30093..30935)
                     /locus_tag="O2692_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4297844.1"
                     /translation="MTTLLNVDSVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSITFFGEEHTVDDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYMTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMEILEDFVAQGGGILISSHISEDIEHLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTEDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            complement(30960..31673)
                     /locus_tag="O2692_000195"
     CDS             complement(30960..31673)
                     /locus_tag="O2692_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HCZ4297845.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGFGIENLTFATYNNVV
                     VISIVATIIIILVEAIVLMNQRRALKYKQLVDKEVDIDATDQYELLANRYVLNGSILS
                     ILQTVIAFLVLLIFVVGHAAANAILFFLIPFFASAIFNTQFALFNRRFDDRIPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIVVIGLYSITTGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            complement(31670..31873)
                     /locus_tag="O2692_000196"
     CDS             complement(31670..31873)
                     /locus_tag="O2692_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4297846.1"
                     /translation="MRNRLKELRARDGLNQTQLAKQAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEA"
     gene            32114..32503
                     /locus_tag="O2692_000197"
     CDS             32114..32503
                     /locus_tag="O2692_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCZ4297847.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAQKSNKTIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLQDQAVISELIPQ"
     gene            32603..32827
                     /locus_tag="O2692_000198"
     CDS             32603..32827
                     /locus_tag="O2692_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HCZ4297848.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            32844..33545
                     /gene="tatC"
                     /locus_tag="O2692_000199"
     CDS             32844..33545
                     /gene="tatC"
                     /locus_tag="O2692_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HCZ4297849.1"
                     /translation="MVHFSELRHRLVKILLSFVITVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFS
                     VLLFCAGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGLLFQL
                     PILFMGLAKFGLIDTTSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFS
                     MFIVKFTCRGKPPTH"
     gene            complement(33637..35349)
                     /locus_tag="O2692_000200"
     CDS             complement(33637..35349)
                     /locus_tag="O2692_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCZ4297850.1"
                     /translation="MKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSESNAVKSDVRKLEDAKANDNQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSNTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIETWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVKLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQGAYIMVVAILIF
                     KFKK"
     gene            complement(35330..36559)
                     /gene="efeB"
                     /locus_tag="O2692_000201"
     CDS             complement(35330..36559)
                     /gene="efeB"
                     /locus_tag="O2692_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCZ4297851.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKNNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            complement(36556..37410)
                     /locus_tag="O2692_000202"
     CDS             complement(36556..37410)
                     /locus_tag="O2692_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297852.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDIIPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            complement(37678..38223)
                     /locus_tag="O2692_000203"
     CDS             complement(37678..38223)
                     /locus_tag="O2692_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4297853.1"
                     /translation="MFGMKVNEQITLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKE
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
     gene            38642..39637
                     /locus_tag="O2692_000204"
     CDS             38642..39637
                     /locus_tag="O2692_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCZ4297854.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSKLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            complement(39697..40263)
                     /locus_tag="O2692_000205"
     CDS             complement(39697..40263)
                     /locus_tag="O2692_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCZ4297855.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQQVFEEENNQYDF"
     gene            complement(40277..41338)
                     /locus_tag="O2692_000206"
     CDS             complement(40277..41338)
                     /locus_tag="O2692_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCZ4297856.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNILPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            complement(41352..42278)
                     /locus_tag="O2692_000207"
     CDS             complement(41352..42278)
                     /locus_tag="O2692_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4297857.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGI
                     KSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPQMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPVEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            42586..43944
                     /gene="glpT"
                     /locus_tag="O2692_000208"
     CDS             42586..43944
                     /gene="glpT"
                     /locus_tag="O2692_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCZ4297858.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            complement(44027..44467)
                     /locus_tag="O2692_000209"
     CDS             complement(44027..44467)
                     /locus_tag="O2692_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HCZ4297859.1"
                     /translation="MCKSKILLKNIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKSGYFVTCWTKDEDNYNRPYKIEEFADYLIVAVIDDELNGYFLFPRELLV
                     EKGILASSKYQGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(44571..45926)
                     /gene="mepA"
                     /locus_tag="O2692_000210"
     CDS             complement(44571..45926)
                     /gene="mepA"
                     /locus_tag="O2692_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HCZ4297860.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMVSMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMSAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            complement(46033..46452)
                     /gene="mepR"
                     /locus_tag="O2692_000211"
     CDS             complement(46033..46452)
                     /gene="mepR"
                     /locus_tag="O2692_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HCZ4297861.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            46664..48619
                     /locus_tag="O2692_000212"
     CDS             46664..48619
                     /locus_tag="O2692_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCZ4297862.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            48624..49067
                     /locus_tag="O2692_000213"
     CDS             48624..49067
                     /locus_tag="O2692_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCZ4297863.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            49069..49353
                     /locus_tag="O2692_000214"
     CDS             49069..49353
                     /locus_tag="O2692_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCZ4297864.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            49368..50714
                     /locus_tag="O2692_000215"
     CDS             49368..50714
                     /locus_tag="O2692_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCZ4297865.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAIAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATTILLKGRQKEQQ"
     gene            complement(50807..51832)
                     /locus_tag="O2692_000216"
     CDS             complement(50807..51832)
                     /locus_tag="O2692_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HCZ4297866.1"
                     /translation="MNNKVLATGGTGFVGMRIISRLLEQGYDVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAVDS
                     MAKYHLI"
     gene            complement(52147..53169)
                     /locus_tag="O2692_000217"
     CDS             complement(52147..53169)
                     /locus_tag="O2692_000217"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCZ4297867.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIYGDFFGQGDIKDVEEALQGTKMTREDLTHQLKQLDIVYYFSNVTVEAL
                     VDMILS"
     gene            complement(53147..54091)
                     /locus_tag="O2692_000218"
     CDS             complement(53147..54091)
                     /locus_tag="O2692_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HCZ4297868.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDVADYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLHRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITAALRNDSTTKNNNIGETEDVLNRTD"
     gene            complement(54081..54881)
                     /locus_tag="O2692_000219"
     CDS             complement(54081..54881)
                     /locus_tag="O2692_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HCZ4297869.1"
                     /translation="METLKSNKARLEYLINDMHRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LSAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            complement(54882..55214)
                     /locus_tag="O2692_000220"
     CDS             complement(54882..55214)
                     /locus_tag="O2692_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCZ4297870.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            complement(55248..56249)
                     /locus_tag="O2692_000221"
     CDS             complement(55248..56249)
                     /locus_tag="O2692_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCZ4297871.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEDFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEKVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            complement(56363..56455)
                     /locus_tag="O2692_000222"
     CDS             complement(56363..56455)
                     /locus_tag="O2692_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297872.1"
                     /translation="MESIFKIKLMNGICRSENMNMKKKNKGEKI"
     gene            56551..57750
                     /locus_tag="O2692_000223"
     CDS             56551..57750
                     /locus_tag="O2692_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCZ4297873.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            57823..58650
                     /locus_tag="O2692_000224"
     CDS             57823..58650
                     /locus_tag="O2692_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4297874.1"
                     /translation="MTEIKDKVITKDAFALPYTIIKAKNQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTNSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMM"
     gene            complement(58892..60964)
                     /gene="lip2"
                     /locus_tag="O2692_000225"
     CDS             complement(58892..60964)
                     /gene="lip2"
                     /locus_tag="O2692_000225"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HCZ4297875.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTSTN
                     AAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQSTQ
                     NSKTIRMQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQ
                     SNDVDKSQPSIPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTH
                     QPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAP
                     KTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKF
                     KVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKT
                     YKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGG
                     HNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFK
                     QLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSS
                     HTGPLGYENPDLGTFFLMATTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTN
                     DEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATES
                     KGTQLKAS"
BASE COUNT        19500 a        11615 c         9261 g        20831 t
ORIGIN      
        1 tagtatgtta cttttaaatg ggatggcttc tgtgtacttc taaatagatt catcttaata
       61 agtccattca atttaaatgt ttggctgtgt tagtaactga cgaatcgcac aagtgcacac
      121 tcatattcac atttactaca aacgcctttt tgctcaaaca aaaatatttg tttacggtag
      181 cttttgaact accacctgca cacacgaaca ctcaaaagat gctaattttg attcgacaga
      241 attttttaca acgtgtttgt tcgaaacgtc ttttcaaggt tgatcattta ttatcttttc
      301 attcaataaa tatgaggtta gcaattttta tcaaaccgcc atagccacat ctcgattcaa
      361 ggataaattt gttttctcat atcttatctc tcatgacagt gcacgatata tatcttttcc
      421 aaatattcat cacttttttc ctgttaaaat catttctatt attaatgctt taatgacaac
      481 ttatttaatt tattgcattt aatgccctgc ttcattttca aatattaact ttaatcgact
      541 agctcatcac actatttacc ctttccacct atcactactg aattcaaaac gatttgttta
      601 caaatcccct tatgccaacg gtcgaatttc atcatgttta catttaattg caaatgccct
      661 cttgaaatat tttaaaatta atattattct tagcatacta gtcggaatta tattcaaaaa
      721 tttgaggagt gacgttcatg aatgcttttc ttacactgat taatattatt gtattggtga
      781 ttttcatcgt aatattgcac atgatggctc gcaaacatat atcttttgcg aaacgtgtgt
      841 ttacagcttt agggattggt atagtattcg gtgtgttatt gcatcttatt tatggcacac
      901 attcgaatgt tattacatca acaagtgact ggttcaacat tgtcggacaa ggttacgttg
      961 cattgttgca aatgattgtt atgcctttaa ttttcatttc tattgtagct gcattcacta
     1021 aaattcaaat cggagaaaag tttgctaaaa ttggtagtct catttttatc tttttaattg
     1081 gcactgtcac cattgctgcg attgttggtg ttgtgtatgc cttggtgttt ggtttagatg
     1141 catcaacaat taaccttggt aacgctgaac aagcacgtgg aagcgaaatt gctaaacaag
     1201 cgaaagattt aacagcacat acgttaccac aacaaatttt ggaattattg cctaaaaatc
     1261 cattcttaga tttcactgga caacgtgcaa cttccacgat tgctgtcgtg atatttgctt
     1321 cgtttatagg atttgcttat ttacgcgtag cacgcaaaca acctgatcat ggtgaattgc
     1381 ttaagcgtgc gattgacgct atctattcac tagtaatggc gattgtgacg ttcgtcttac
     1441 gtttaacgcc atacggtgta ttagcaatta tggccaacac attatcgaca agtgactttg
     1501 gcgccatttg gactttagga aaattcttaa tcgcatcgta cgctgcatta attacgatgt
     1561 atatcattca cttaattatt ttaagcctgt taggtattag cccaattcgc tatgttaaaa
     1621 agactttgga agtattaatc tttgcattca catcacgctc aagcgcgggt gccttaccat
     1681 tgaatgtaca aacacaaaca agacgtctag gcgtgccaga aggtattgcc aatttcgctg
     1741 cgacatttgg attgtcgatt ggacaaaatg gttgtgcagg catttaccct gccatgcttg
     1801 caattatggt cgcacccgtc gctaatgtag aaattgattt acaatttatt gtaacgttaa
     1861 tcgctgtcgt gattattagt tcattcggcg ttgctggcgt cggtggcggg gctacatttg
     1921 catccatatt ggtgttgtca acgttaaact tgccagtcgc tttagccggc gtgctcatct
     1981 ccgttgaacc attgattgac atgggtagaa cagcattaaa cgtgaacgat tcaatgctag
     2041 ctggtacagg taccgccaag ttaactaaac attgggataa agatactttc gattcaaatg
     2101 ataatgctgc attaacttca cactaattca ttgcaagaaa tacccgcccc ctctagctat
     2161 acttatcatt ttaagctaga ggggggcggg ttattttggt atcttgctat cgctgtatta
     2221 agcctgattt ttgcaattgt tctaacatat ctaatcttgc tttgtttcct aggaacatct
     2281 caatttgctg cgaccatgtt tctttgcgat tcccgtttgt acgttgatca tagtactcgc
     2341 tgattgtctg gtcgtaatct gccatttgtg catactgtgt ttccttatca gcatgatact
     2401 tattatgatg gaagacatgg tcaaatggta agcgtggctt ggctgcacca ttttcgtcat
     2461 ctgcgggttc ccctactgcc ataccaaata ccgggaagac atagtcaggt aagtctaaaa
     2521 tttctcgaac gcgttcaaca tcatttctta atgatcctaa aaagacaatg ccatacccca
     2581 tatcttcagc agttaccgca atattttcgg caactaatgc tgcatcgatt gcacctacta
     2641 gcaaaccttc cgttgaacca tatgcatttt ccatatcagt ttcagcatgt tgatcaacta
     2701 aatgatgacg ataataatca ataacaaaga cgaataaata gccattttct acaacataag
     2761 gttgtccaga aacttctcgt aaattttctt taatcttctc atcgtcgata ccaataattg
     2821 agtatgcttg caggaaactt gacgtcgaag cgctttgtcc agcttctacc aatttcttaa
     2881 caacgtcttc acttaaaggt ttattcttaa attttctaac agaatgatgc tttttcacaa
     2941 gattatatac atgttctgac acgttaccca ctcctcgata ctttacaatt aatttaaaat
     3001 cttcatattg caattgtaca cgtccaaact tttttatagc aattatttgc ttataagtta
     3061 ataattgaac tttttaaaat attgtctcat actttaaaaa tccagtttct cactcattac
     3121 cttcatccat ctcgaacaaa tattctcaca ccattatcaa taaggttgac ttagttgaga
     3181 atacaaatct tcttttttta cattttttac attaaaatca agatattaaa tatagacaca
     3241 accttttaaa tcatcaacta caaaatccat tatcaaagca ctttatatta tctttcttta
     3301 ttttaaaagt aatcattcac tttacaaagt tatccagttg actattctgg atcaaaatgt
     3361 aataatgcta actaagagat tagaattatt ataattaagc gcaatcaatt gatagttcta
     3421 attcatactt aaattcttag gaggaagata tttatgtcat taattaacaa agaaatctta
     3481 ccatttacag cgcaagcttt cgatccaaaa aaagatcaat ttaaagaagt tacacaagaa
     3541 gatttaaaag gttcttggag cgtagtatgc ttctatcctg ctgacttctc attcgtttgt
     3601 ccaactgaat tagaagactt acaaaaccaa tatgaagaat tacaaaaatt aggcgtaaat
     3661 gtattctcag tatcaactga tactcacttc gtacacaaag catggcatga ccattcagat
     3721 gcaattagca aaatcactta cactatgatt ggtgacccat cacaaacaat cactcgtaat
     3781 tttgatgtat tagatgaagc tactggttta gctcaacgtg gtacattcat tatcgaccca
     3841 gacggtgttg tacaagcatc tgaaattaac gctgacggaa ttggccgtga cgctagtaca
     3901 ttagctcaca aaatcaaagc agctcaatat gttcgtaaaa accctggcga agtatgccca
     3961 gctaaatggg aagaaggcgc taaaacattg caacctggtt tagatttagt aggtaaaatc
     4021 taaggaggca ttacaaccat gcttaatgct gatttaaaac aacaacttaa acaactatta
     4081 gaactaatgg agggcaacgt tgaattcgtt gccagccttg gttcagatga taaatccaaa
     4141 gaacttaaag atttgttgac agaaattact gatatgtcac ctagactatc tctttctgaa
     4201 aaatctttaa aacgtacacc aagtttctca gtcaatcgtc ctggcgaaga aacaggtgta
     4261 acatttgcag gtattccatt aggtcacgag tttaactcac ttgttttagc aattttacag
     4321 gttagtggtc gtgcacctaa agaaaaacag tcaatcattg accaaattaa aaaattagaa
     4381 ggttcattcc attttgaaac attcattagt ttaacgtgtc aaaaatgtcc tgatgtcgtt
     4441 caagcactta acttaatgag tgtgatcaac cctaacatca cgcattctat gattgatggt
     4501 gcagtgttcc gtgaagaatc tgaaaacatc atggcagtcc ctgctgtctt tttaaatggc
     4561 gaagaatttg gcaatggtcg tatgacaatc caagatattc tttcgaaact aggcagtacg
     4621 gcagatgcat ctgagtttga aaataaagaa ccttatgatg tcttaatcgt tggtggtggt
     4681 cctgctagtg gtagtgcagc gatttacaca gcacgtaaag gtttacgtac tggtatagtt
     4741 gctgatcgta tcggtggcca agttaatgat actgctggta ttgagaactt cattactgtt
     4801 aaagaaacaa ctggttctga attttcttct aacttagcag cgcacattga tcaatatgac
     4861 attgatgcaa tgacaggtat acgtgctaca gatatcgaaa agactgacga agcaattaaa
     4921 gttacgttag aaaacggtgc tgtcttagaa agtaaaacag tcattattgc tactggtgca
     4981 ggttggcgta agctaaacat tccaggtgaa gagcaattga ttaataaagg tgttgcattc
     5041 tgccctcact gtgacggacc tctatttgaa aataaagacg tagcagttat cggtggcggt
     5101 aactctgggg ttgaagcagc aattgacctt gctggtatcg ttaatcatgt tacattattc
     5161 gaattcgcta gcgaattaaa agcagacaac gtgttacaag atcgtttacg ttctttatca
     5221 aatgttgata tcaaaacaaa tgccaaaact actgaagttg tcggagaaga ccatgttaca
     5281 ggtatacgtt acgaagacat gaacaccggc gaagaacatc tacttaactt agatggtatc
     5341 tttgttcaaa ttggtttact tccaaacaca tcatggttaa acgatgctgt tgaattaaac
     5401 gaacgtggtg aaattgtgat tgatcgtaac aataatacga atgttcctgg aatatttgct
     5461 gctggcgatg tcacagatca gaagaacaaa caaattatca tttcaatggg cgctggtgca
     5521 aatgcagcat taaatgcctt tgactatatt atcagaaact aaagtttcat tcatttcact
     5581 gacagtatct tacatttgtt aagatgctgt tattttttta tcccgacata aaatcatgtt
     5641 atgataaact taatatatac aacgagatgt gcgataatgc ttaaagtatg tacctctcgc
     5701 aaacaaatca atatgaaaaa tgatattgag aaagaaaagt atgtgaacga aattattatg
     5761 cttttacgta cgatacctac gtctaaatta gtacaaccaa ttatgcagtt atttatagat
     5821 aaagctatcc ttaacaacgt ccaaaatgat gcattattga tagaaaaagc aactcatgca
     5881 cgtaatcatt tacgtctatg gtcattgcaa ggtcatcgtg acattccaaa ctaattcaat
     5941 gttactctat taaacatgaa tacatatttt ttcaattaaa gataaactaa ggagttatat
     6001 tacattatga aaaagagatt actactaagt acatttttag catcgacatt aattcttaca
     6061 ggttgcgcgt ccgatcaatc tgataacgaa gatcatcata caagtactgg catccatgca
     6121 cctaaaagcg ctaaaaaatt agaaacaaaa gatatcttta actcagataa gaaaaatagt
     6181 gatattagtg atgcagagat gaagcaagct atagaaaagt atttgtctgt aaatagcgat
     6241 atacttgata acaaatatat tatgcaacat aaactagata aacaaattga cagtcaaaca
     6301 aaagtgactg aaaaacaagc agaaacactt agccacttat ctaacttagc tgttaaaaac
     6361 gatttgcatt tcaagaaatt tgtcactgaa aacaatattc ctaaagaata taagaaaccc
     6421 gttgaattaa tgatgaatta cttcaaagct ttaaatagta caatcgccaa tgttgatgaa
     6481 gatattgaaa agcttagcta tcaaccgcaa aataaaatca atgttgtcga cgtgccaaca
     6541 aaatatgccg gtgatgttaa taaaaagcaa caagataaaa ttaaagattt cttaaaatct
     6601 aaaggtatca agagtgatgt tatcgataaa taactgaaag ccatcattgc gaaatgtaac
     6661 acatcattac atgagtttta attcatgaca attctttatc tttaaaagtg actaatcaat
     6721 ttagctaaag tcatcatttg ggcatcaagc agcatattac tgtttgatgc ctttttaatt
     6781 gccttaaatt ctctatctat gccaaaattc ccattagttg acgcatttga ctatatacct
     6841 aacatattcc atacttatat tgactctata aacaaaggat gatcatgatg aaagttcgaa
     6901 ttacttttat tattttagca attttatcga cgattgtttg cttgttctta gcagcaatgc
     6961 atccaacagg accaaacaca gtaacttttg aacagccata tttattcacc ctcaacatca
     7021 ttataatggt attagtcgca ttaccttcac ttgttttagc tatatatgat tatatgagtt
     7081 ttagaatttc ttctgctatt ttacaatttc taggggctat ctcttggttc tttttatcat
     7141 tgatattatc gctcacacaa tttacacctt ttacattagc gtcatttata acttcaatta
     7201 ttttgttcac aagcacaatt atcacattag ccattggtgg taagtctgtt gaaaagaatg
     7261 attcccctta aattccaaat gaaaaaaagg ttctgaaggc cgctataaaa cacagttttt
     7321 cagaacctct atacttctat tcaatgatat atggtttgca attttctacc tttaaatcca
     7381 cagcttctgc ccttgaaact ttgttaaaat aaaccatcaa acaacgaatg acaacttgat
     7441 gtgcaacaat gacaatatca tctttttgtg tatcttcatt gacaacatga ttcataaaat
     7501 gttctacgcg ttgatataca tcttcataac tttctccttc aggcgctttt tgtgaaaaac
     7561 tatgacgaaa gtctttaaag tttggatcat tgaaatattt atcatatttc ggattcgcac
     7621 tgatttcatc tttatattca ccctcaaata cgccaagtga acgttctctt aatagagggg
     7681 tagtcgttga tgcaatgtca tatggaaaaa tatgttcaaa cgtttgctgt gttcttaata
     7741 agtctgaaac atatacatgt ttaatctgtt tctctttaaa ataatcacat aaatcgtctg
     7801 cactttttgt gccagtatcc gttaacggca catctaattg tccacaaaaa taagatcgaa
     7861 aatgtttatt atcataattc gattttgatt cgccatgtct aactaaataa atcgtcataa
     7921 tattactcct taccttatgt atttcatatc taccataaca ctttgactac taattcgata
     7981 tcaatcttaa tattctattc taaaaaaaga attaattcat attttaaatt aatgactcta
     8041 aaaatagtat tgtctttaat caagtgatgt gttactatat gggtgtagtt cttaaatgac
     8101 tacacagtct tttatattca tgaacataat actcctggat ggcgcaaccc aacgtcatcc
     8161 tttttttatt tgtcaaatac tcgctttttt atttccaaaa tttcctcata ttaatttatt
     8221 actttttaat cctatcttat ctacattttc aaatattata atacatattg accattttca
     8281 cgcaaataaa aaagatgaca actgaatgcc atctttattt caatttccaa ttgaattttt
     8341 ttattattta cgcatagctc ttaaaattaa cgttacgatt gcaattaaga taattgaacc
     8401 aattaatgct ggcaagatgt aaatacttcc taattcagga ccccattgtc cgaatagttt
     8461 gccacctacc catgatccaa taatacctgc gataatattg cctaaaatac cacctgggat
     8521 atctttaccc ataatagcac cagcagccca tccaattaag ccaccgacaa ttaacattcc
     8581 aataaatcca aacataattt tcagtctcct ttttctattt attttgcgtt attctaagta
     8641 gtacccctta tttacaattc taaaacaaat tcaaattatt tttatccaaa tatttttaaa
     8701 agtagtaatt gaatatcaat tttattcaat gtagctatcg ttatttaaag tctctgtacc
     8761 gataatatca tatacattta cattattttt tctgccgaat tcatagcttg attattttat
     8821 gttataggac tagaatatac acatattatt agagcatctt tgaattttaa atcaagaagc
     8881 gaggttaatg aacaatgaat atgcatattt tatataactt acgaactaaa cataatttag
     8941 aaattgacga attagcacag caattaaatg agaaatatgg tactaaatat gaagcacatc
     9001 aaatttggga atgggagaat catcaccatg aacctaaatt taaagatgcc atgcatttag
     9061 ctgacttctt tgatgcacca tatgaaatgt ttttagaaag taaggttaaa gaatatcaga
     9121 aacatttaga agaagtcgat attcgcatgg ataaatagat gcaaataaac cctcacaaca
     9181 cgtttggcat atatcctttc aaatctatac tggatatatt acattacgtt gtgagggtat
     9241 tttattaatt aatatgaatt aagacatttt acaagcgtta atgcaacgaa tctttttagt
     9301 gatcttgctc actctttaat actttaccgt tcttagcatc aacagtaact tcttgttttt
     9361 tattaccttt tttcaaatcg atattgtaaa caagtttgcc atcatctttt tcaagtgacc
     9421 attctttaat atcaccatca aattcttttt gtccttcttt aatggctttt ttgtaatcta
     9481 tagcatcgct atatttaaag ttatcatttt cattcattgt atcttctttt tcagtctttt
     9541 tgttaatcac ttttttattt ttatcagcaa caagtacttc tgactcttca ccagattttt
     9601 gttgcgtcac tttataagcc cattcaccat tagaattttc aaatgaaatt cctttcaact
     9661 tttggccttt gtaagtttct tcagcttttt tcacagcatc ttctgggctt gttttaacat
     9721 cttttaaagc aataacatct tttgttgtat tagtgtcttg attagtattt gactctgttg
     9781 attttgtttc atcttttgga gtatcattgc cacatgcagt aagcaccacc gctgacattg
     9841 ataacactgc taatgatttt aatttcataa tatcactctc tcttcctatt tttgaaactc
     9901 ataacaaaag cttatatgct atatagattg tattacccct tgtttttaat tttattcata
     9961 attattacaa atatttttaa attaatcgtc atgcgttact ttcgttcgta ctccttttat
    10021 aaatgaacca tgtaatataa gcatgctatt atcgactcag tttgtctaat gctttctttg
    10081 gtacttcttc cttttcaact tcttcaaaag tttctacatg atgaccttta tgtgtaattt
    10141 tcaagtatct atgcggttta acatcaaatg tagcagtata cattaattgc gtctctttcc
    10201 cttctttatt aaaaacactt tgactataac ttcggagttg atcatccatt ctagtagaca
    10261 cctctgtcgt tttaacataa gaatcatcct tctttactaa tgggttaaat tgatctgtta
    10321 taccatggga gtctattgtt tttaaaatga ataaagcagc ataagcgcca atgatagtaa
    10381 gtacaagata tgttattgtt tttaaaatcg ttttcaaaag atcacctctg acaaaatata
    10441 aaataggatg tatctgaaaa caacttacat aattgtaatt aaagtgtaag tattaggaga
    10501 aaatgatggt atttgaatct gaaagatacg aatcgttttt aaactaagtt attttataga
    10561 gactttatta tgaaattgaa agaagttaat tttaaggttg tcgaatgtaa aaacatattg
    10621 attaaccata aaagagaaat tatttttgta gatttcggca taaacgtcat taaagaattt
    10681 tctatatgat ggtgctaatc ataacaaaga aagctaggca cctttagata cctaactttc
    10741 aaagtattag cgataaaaca ctatatatac taatttagct taatttcaaa cttgttcaat
    10801 gtcattaaca cttttcacaa gtttgaaagt tacatttttt atatcggctg tattaatttt
    10861 tacattttcc ctatcttttt gtagctcttt atgcgactct aatgtatatt taccaccatc
    10921 ttttgtattt atgacgatgt tacctttagg caaatgttct cccatataca agccatagga
    10981 aatatgagca aatcttatga tatgatccaa ttcttttaaa gtgacaactt ctttattaag
    11041 agtaatattc ctcttaggtg tatacactct gccagcaact gtgtatgttc cttctaaatg
    11101 tatacgaact tggttttcat ttaaaggtct ctctgaataa acccaattcc tagatttatt
    11161 agattttcca tttattgtat ccttgctgta tctatcgtaa agttttttaa ccaaagcttt
    11221 ttctgaatta tttggtactt cttcaacttt ttgtaattgt ttatcttgta cacttgagga
    11281 attttgtgtt gacgcgtctg cttttggata tattccaaat gttcccccat agatgatacc
    11341 tagcgataga atagatttta atacgattga attttttgag tcatattttt tgaacatatt
    11401 taattacctc cttgatgtaa agctttattt gctacaatta taaaaataat agacgtgttc
    11461 atgaattaaa ttcatcttaa ctcttgatta actttaattt gctaccactc tgaatttaat
    11521 aactataaat cgtctacaca taattggaca aaatctaaga gaataaaatt tgttaattta
    11581 aaatagcaag caattcaaag ttatatgtgt aatagataaa atagatatcc ctatagtgat
    11641 gcgttactag ctaaacataa taacacatta gaagataatg aagttaagga gttactggat
    11701 tgtttcgact atgtaattaa gtataaaaat atccaacgac aaaacgtaat tataaaatgg
    11761 taaaagctat ggtacagttt caaattgcta atgacatgcg tatcggtgag ctacttgcaa
    11821 taaagagagt aaatataaac tatgaagata aaacgctaga tatcgacggt aaagttaatt
    11881 ggataactga aaaaagacgg gagcattcgg agtaaaggag acaactgaaa gaagtaatag
    11941 ctataaggcc acagggctca ctacccaaag catcgactta ctaagaacac ttatgcttgg
    12001 aatgataagt ttattgatag agagtacata ttcacaaata cgtctggtag ccctatcgac
    12061 tcgaacaaaa ttagccacat tattaaaggg gggcgctgat attagttcta ttaagaaacc
    12121 tataacgacg catacattac atcattcgca tatatctaca cttgctcaat taggaattaa
    12181 cttaaaagca atgcaagagc atgtaggtca ttcagattat aaaaaaaatc tagagatata
    12241 cacacatgtt actaatcaga tggcgaaaga tatgatgaat aaatttgaac gattggggag
    12301 ttaaaattgg aaaaagatga tacactagca gaaattaagc ctatgctcaa ttttgatgag
    12361 caaatagcga aattaaaaca gatgaatata ttttttaata ttattgacac cgaaaaagca
    12421 aatgaaattc ttagaaaaaa taattacttc ttcaaactag cttatttccg aaaaaatttc
    12481 gaaaaaaaga atggcggcta tttcatagaa tttgcttatt tatcagattt agcaactata
    12541 gatatgaaat taagatacac aatgttgcat ttaactttag atattgaaca tagtttaaag
    12601 tatctagtct taaaactaat aacagaaaat aaccaagaag atggttataa aataatagat
    12661 gagttcttat gtattgataa atcatatagc aattcaaatt ttgacacaaa ttcaagaaca
    12721 ccagaagaag ttatggaaac caaaatcaaa aataaaaacg aaatattcaa gcatatgaat
    12781 aaacgaggac aactacccga gaagttgaat aaatactatc aaaatccacc cgcatgggtt
    12841 tgcattgaat tcatgcaact aggtcaattc gtttcgtttc tcaacttcta ttacaagaag
    12901 tacaatgacg aagaattgag agttgctaat attttaatgc ctttagttaa aaatataaga
    12961 aacaaatcag ctcataacca acccatcata gcaaatctaa attatgacag tagattacct
    13021 caatatttat ttgaaaaagg gaataatata ggcatatcta gaaacatgtt cggaataaaa
    13081 aatttcatag atactttcgc tacgctagaa ttacataatc aagtttgtag taatgcaatt
    13141 atccaagcaa gatatcacga tttggaccaa cttcaaaagc gatataaaag aaacgaaagc
    13201 tattataata atgcattagc tatcaaaaga ttttttatag ctttagataa aattattgac
    13261 ttcaacagac caaaagtata aactatctag tgaggaaaga gacttatagg tctcgcgagt
    13321 tattttaatt cgtatgcaag aaaaagaaga gctatgcatt ttatttaaaa tgcgtagttc
    13381 tttttttatg catctaaatt catattattt ttgcaatata aacatatctt tgtgcaaatt
    13441 ccgaacacaa aacattcaca tcatcctttt ttgccctttt tctatacccc aaaacacaaa
    13501 aagccccgta agcctatgcc tacggggttt gacaataaat tatatattat tgttcttctt
    13561 taacatatgg taataatgcc atatgacgag aacgtttgat agctgtagtc aacatacgtt
    13621 gatatttagc tgaagtacca gttacacgac gtggtaaaat tttaccgcgt tctgagataa
    13681 aacgttttaa taattcagtg tctttgtagt cgatatgtgt aataccattt gctgtgaaat
    13741 agcatacttt tttacgacga cgtccgcctc ttcttggtcc acctgccatg attgtgtgcc
    13801 tcctttgata attttttcgc tttaatttcg ttaattttta ttagaatggt aagtcatcat
    13861 cacttatatc aatcggtccg tttgcatttg caaatggatt atcagattgt ttcgtgtttg
    13921 atgaattatt gtacgaattg ttttgtcctg attgttgacc accgaatcct tgaccgtaat
    13981 cttggaattc attttgttga cgttggccac cattttgttg cgcattttta ggttcaagga
    14041 attgaacgct atcacacaca acttcagtaa caaacacacg acgaccttct tgattttcat
    14101 aattacggga ttgtaagcga ccatctacac cagctaaact acctttagat aaatagttat
    14161 ttacattatc tgcttgtctt ctaaaaacaa cacagttaat aaaatctgct tcgcgctccc
    14221 cttgagcatt cgtgaacgta cgatttactg caagagtgaa tgtcgctaca ctcacacctg
    14281 agggagtggt tctgtattcc ggatctttcg ttaaacgacc tactaataca actctattta
    14341 gcatttaaac gccccctcta attattactt gtcttcgtct tcacgaataa ccatgtaacg
    14401 aatgatatcg tcactgattt tagctagacg ttggaattcg tcagtagctt tgttgttatc
    14461 agatttaaca cgtacgatgt tgtagaagcc atctttgaaa tcattgattt cataagctag
    14521 gcgacgttta ccccagtctt ttgcttctaa aacttctgca ccttcagtag ctaagatacc
    14581 gttgaaacgt tcaactaacg cttttttagc atcttcctca atgtttgggc gtacgatgta
    14641 cataacttca tatgttctca ttttatattt gcacctcctt gtggtctata cggcttatca
    14701 atcttaaaac agataagcaa ggaataattt tcattactca caataaagaa ttatatcatg
    14761 cgccattact ttttacaata ataattcaaa ctactcttca tatcattttt gatattaatt
    14821 catttgaaac tttctatgat atttttaaaa atacacttca caaaagcgaa catatgttct
    14881 ataatagttg tgaggtggta aggaatgaat ttaaatattg attggtctaa agattttcaa
    14941 gaattccaag agatacttaa tagtggtatt catcctgaat ggctttattg tgcaaaggct
    15001 aatcttgttt tagagcctgc ttatactggc gaaggcaaac aatttttcag cactcaagat
    15061 attattaacg cgagtaaaat tattccattc ttttaataaa ttatacgcat aattaaaaga
    15121 ggcgaacaat tgattccgcc tcttctacat atatactatt taattaactt cactctatcg
    15181 tttatacatt aaatctgaaa tgaacgatat cgccatcttg cataatatat tctttacctt
    15241 ctaatcgttg tctgcccgct tctttagcgc cactttcgcc accatattgt acatagtcgt
    15301 cataacttgt tacttcggca cggataaatc cacgttcaaa atcagtatga atgataccag
    15361 cacattgagg tgcagtcata ccttgtttaa atgtccaagc acgtacttct tgcacaccag
    15421 cagtaaaata tgttgataat cctaataatt cataagttgt tctaattaat cgatctaatc
    15481 ctggttcttc gatacctaaa tcttctaaga acatttcttt atcttcatca tctaatgtag
    15541 caatttcttc ttcaattttt gcactaataa caatcacttc agagtcttct tgcgctgcat
    15601 attcacgaat cgcttttact ttatcattat catcatcacc aatttcatct tcaccaacat
    15661 tagcgatata aagcattttt ttagaagtca gtaattgcgc ttgattcacc catttttgat
    15721 cttcttcatt aaagtcaata ctacgagcgg gtttaccatt ttctaaagct tctttaatag
    15781 ttgttaaaat acgtacttcc atttcagcag tcttatcttt ttgacgtgct aatttttcaa
    15841 ttctaggcaa acgtttctca acagattcta agtccgctag tactaattcc atattaataa
    15901 cttcaatatc atcaataggg tctactcgac cagcaacatg agttacgtta tcatcatcaa
    15961 atgcacgaac gacctgacaa atcgcatcta cttctctaat atgtgataag aatttattac
    16021 ctaacccttc tccctttgaa gcacctttca caataccagc gatatctgta aattcaaatg
    16081 tagtcggcaa tgtcttttta ggttgaacca tttcttctaa tttaagtaat ctagcatctg
    16141 gcacttctac tatccctaca ttaggatcaa tcgtagcgaa tggatagttc gctgctaaag
    16201 cacctgcttt tgttattgca ttaaataatg ttgatttacc aacgtttggc aatccaacga
    16261 tacctgctgt taaagccatt aatcattctc ctatctttgt gtatcatcat gagattcgat
    16321 gattttttta agttttttat caaacgtttg acgtggaatc ataatacttc tttgacaatt
    16381 ttcacattta attcttatgt ctgcacccat tctaataatc ttaaaacggt ttgttccaca
    16441 cgcatgttgt tttttcattt ctactatatc atttattcca tattttgacg ccattaaata
    16501 tgacctccat gtattataaa ctactaaaca ccgttttcac tatgattata agcagtcata
    16561 attggttgag gtgttttaat accttcttgt aaaaacattt tttgtacttc tttgcgaata
    16621 attcgagctc cagcaaatcc ttcacctgga attgtttctg cagatattct caatattact
    16681 cttgtatctt caatagcatc aataccaata acaaccggat cactaacaaa taagtaatat
    16741 ttactacgta aagaagtaaa tagtttgttt agttttttat caacattatc aatgttttct
    16801 tcgacagaca ctggaatttt aacgatagct gtaccatttg taattgagta gttcgttatt
    16861 tcacccatac tactatttgg taaaattgtt aattctcctg aaattgtatt gattcgtgtt
    16921 gaacgaagtc caattgattt aactgttccc tctgctacag ttgtaccacc gttatttatc
    16981 ttaacataat cacctacatc aaattggctt tcaaatataa taaagaatcc tgtaattacg
    17041 tctttaacta tagtttgagc accaaaacct actgctaagc ctacgacacc agcactggca
    17101 attacccctt caacactaat gccaaattta cttaaaatcg tcgtaataac tataaaccaa
    17161 acgatatact tcactacatt ttgaacaaga gatattaaag ttttagagcg ctttttgtta
    17221 ctctttttac ttttattttg aatcttaaat ccctgttcaa tcattttatt cagtatttta
    17281 ataactatga gggctacgat aatataaata acaatcatag cgattttagt tgcaatgttt
    17341 tcatatgttt ctatttttgt taatggctca aatagagatg aaataatatt catgacttga
    17401 ttcatgataa gcgttactcc cttctatatg atatatcaat attgtattca tatttttata
    17461 tacttccaac ctacgaaagt ctcaatatat ctagaatcaa atgttcactg attacaattt
    17521 atttacactt gcacaaagag ttattatata aaattgtgta actatttacc atacctacga
    17581 tttaattgtt caattattct cacaaaatct tcttgtgaat caaactcaaa tgagatttta
    17641 ccaaccgatt tttttattga tatatctact ttggtaccat actgttctcg caactgtcgt
    17701 tcttgctgct ttataaattt aggcttagtt atatctactt tgtctgtttc cgactttgac
    17761 gaaacatttt ttaattcatt aacatggttt tctaaatatc tgacactcca cttttcttta
    17821 acaacccgtt tcgctaacct aagcatttgt tgttcatctt taattgccaa taacgttcgt
    17881 ccatgtgcac ttgtcagtcg cccatctttt accatgtcag caatcttttt cggcaaatgt
    17941 aataacctca acatattcgc tatatacggg cgcgacttac tcaatcgttt cgctacttct
    18001 tgttgtgtaa ttttcaaatc tgtcatcaaa cgttgataac tttcagcttc ttcaatcgca
    18061 tttaagtctt ctcgttgtaa attttcgatg accgccagtt ccatcatatc ttcatctgtt
    18121 aaatctttga taatcgctga tacgtatttt agaccagcaa ttttcgaagc tctaaacctt
    18181 ctttcaccaa ctacaatgta ataaccttga actgtttttc taagcacaat tggttgcaaa
    18241 attccatatt gcttaattga atctgccaaa tcatttaaat gattttcatc gaaagttttt
    18301 cggggctgat acggattcgg tttgatatcc gatatatcta tttgcttaat ttgttcatct
    18361 tttgttttag taatacgttg atcttcactt tttgacaatt cactcacatc actttttatt
    18421 tgataacaac agttaaatat attttatgta ttctaaatga gtcagacaac cattaaccac
    18481 actttattta aaaaataatt catttttcaa cttcaagata ccaattacaa tttcacaaac
    18541 aaattaactt ttaaatcaaa attttctgaa taattgttga caaatctttt tttactctgt
    18601 aagctaatta atatcttata acagtttaat gaaacgtaaa cacaataaag aggaaagtaa
    18661 aacacaccct gcttatacag agagtcttta gtagctgaga gaagattttg aaagcgtgtt
    18721 tgaaaatggc cttggagtgt tgatgccaat atgaggtgtc tacgggttcg cccgttatag
    18781 cgatacagta ttaacattga tgttaaatgg cgtactggat tctttacgca cgattttttg
    18841 ttaataagta tgggatagca cattactata tccttactta ctgactttaa ttgtgataat
    18901 tgttcagtaa gcatatttac ttttaatgcg tactgaataa ggttatttca gcgatggaat
    18961 aacaaataaa ggtggtaccg cgaaacataa gctttcgtcc tttttatccg attcattcgg
    19021 gtacgaagga cggaagcttt ttttattttt tcttatagga gggtcttaat atgaaggata
    19081 cacagttagc ccaaatcaca ttaaccgatg attcaaccgg tgctatagcg aatccaatcc
    19141 atttatctac tgcctacaag catccaaaac taggacaatc gacaggtttt gattatacac
    19201 gtactaaaaa tcctacacgc tcaacatttg aaacctgttt tgccaaactt gagcatggta
    19261 ttgcatcatt cgctacatca agtggaatgt cagccattca attaatatgt aatctattta
    19321 aacctcatga tgaaatttta gtttcattcg atttatatgg tggcacattt agattatttg
    19381 aattttacga gcaacaatac gatatcaaat ttaagtacgt tgattttaca gattatgaac
    19441 aagttgaaaa agaaatcact gataaaacag ttgcattatt cattgaacca atatctaacc
    19501 cacaaatgat tgctattgat gtaaagccat actatcaact ttgtaaagct aaaggcttat
    19561 tgtcaattat cgacaatact tttttaacac cttatctttc aacaccacta gcagaaggtg
    19621 ctgatatagt cttacattca gccacgaaat atattggcgg acataacgat gtactagcag
    19681 gtgtcgtaac cgtcaaagat gaatcactcg cgcaacagtt gtttgatttt cacaacatga
    19741 ctggcgcaac actttcacca atagatagtt atttgttgtt acgtggactt aaaactttgc
    19801 atttacgcat tgagcgtgcg caatcaaacg ctagaaaact tgctaaaaaa tgtcagtcac
    19861 ttcaagcaat tgacgaagta ctatatagcg ggcaaactgg catgcttagt ttaagactta
    19921 acaaggccta tagcgtcgct aaattattag aaaatttaga catttgcatt tttgcagaaa
    19981 gtttaggagg tactgaaaca ttagtgacct tcccttacac ccaaacacat gttgatatgc
    20041 cagatgctga aaaagataaa cgtggcattg atgagtattt aatccgctta tcactaggtg
    20101 ttgaaaatta tgaagacatc gaacgcgata tcatccaagc attagataaa gctcagattg
    20161 gagagattgt atgacacttt caaaagagac agaagtgata ttcgattggc gtagaggtgt
    20221 ggaatatcat tcagctaacc caccactcta tgattcttca acattccatc aaacaagtct
    20281 tggtggcgat gttaaatatg attatgcacg aagtggcaac cctaaccgtg aacttttaga
    20341 agagaagtta gcacgattag aacagggaaa attcgctttt gcttttgcat caggtattgc
    20401 tgctatttca gcagtacttt tgactttcaa atctggtgat catgtcatct tacccgatga
    20461 tgtatatggc ggtacttttc gcctcactga gcaaattttg aatcgattta atattgaatt
    20521 tacaaccgta gatactacaa agctcgaaca aatcgagggt gccattcaat caaacacaaa
    20581 attaatttat atcgaaacac catcgaatcc ctgttttaaa attactgata tcaaagctgt
    20641 ttctaaaata gccgaaaagc atgaactact ggtagctgtg gacaatacat ttatgacacc
    20701 gttaggtcaa tcacctttat tacttggcgc tgatattgtc attcatagtg ctacaaaatt
    20761 tttaagtgga catagcgatt taattgctgg tgctgtcata actaataatg aggcaattag
    20821 tgacgctctt tatttaatac aaaatggtac aggcaatatg ttatctgctc aggatagctg
    20881 gacacttgct aaacatttaa aaacttttcc aatcagattt aaacaatctg tcgaaaacgc
    20941 tcaaaaaatc gtgtcatttt taataaagca agatgaaatt tcagaagttt attatccggg
    21001 actcactact gctcatttag aacaagctaa aaatggcggt gccgttattg gctttcgttt
    21061 agctgatgag tctaaagcac aacaatttgt cgatgcactg acattaccac tcgtttcagt
    21121 gagtctgggc ggtgttgaaa cgatcctttc acatccagca acaatgtctc acgctgcact
    21181 acctgaagaa gtgagacaag aacgtggtat cactttcggt ctattccgat taagtgttgg
    21241 tctcgaagat cctgatgaac tcattgcaga catcaaatac gcattaaagg aggcattcaa
    21301 tgagtcaatt cctcacacaa ttgaaagata atgttttagt agctgatggc gctataggaa
    21361 ccattttata ctctgaagga ttagacacct gtccagaagc atataatctt agccatccag
    21421 ataaagttga acgcatccat cgttcatata ttgaagccgg tgctgatgtc attcaaacca
    21481 atacttatgg tgcaaatttt gaaaagttaa aacgattcgg tcttgaagat aaagttaaag
    21541 caatacatca agccgccgtt cgcatcgcaa aaaaagcagc aaataaagat acgtatatat
    21601 taggcacagt tggtgggttt agaggtatca aacaagagga tatcagctta caaactattc
    21661 tttatcatac tgaaattcaa atagacacct taattgaaga aggcgttgac gcgctacttt
    21721 tcgaaacgta ttacgaccta gaagagttaa caaatgtcat ttcacgaacg agaaagaaat
    21781 acgacattcc aatcattgct caattaaccg cttcaaacac aaattactta gttaatggtc
    21841 aggcaatcaa tgaaggatta aaacaactcg ttcaatgtgg tgcaaacatc gtgggactca
    21901 attgtcatca tggcccgcac catatgcaag agtctttcac acatattgaa ttaccagagc
    21961 acgcattctt atcttgttat ccaaatgcca gcttattaga tattgaaaat agtgaattta
    22021 agtatagtga caatgcacaa tatttcggtc aagttgctca aaatctaatt cgcgaaggtg
    22081 ttcgtttaat tggtggttgc tgtggtacaa cgccagagca catcaaattt attaaagaat
    22141 ctattcagac acttaaacct gttaatgaca aaaaagtgat tccgatacca acgaaagcac
    22201 ttttcaatcc atctcaaaat aaagttagac aatcattaac atctaaggtt caagaacgtc
    22261 caaccgttat tatcgaattg gatacaccga aacatttaga cacggataga ttttttgaaa
    22321 atatcgctaa acttgataaa gctaatgtag atgcggtaac actcgcagat aattcattgg
    22381 caactgtcag aattagcaat attgctgctg ctagcttaat taagcaatat tacaatattg
    22441 aaccactcgt acatattaca tgtcgagacc gaaacttaat cggcttgcag tcccatttac
    22501 ttggattatc gctcattggc gttaacgaaa tattagccat aactggtgat ccttcaaaag
    22561 ttggtcactt accaggtgca accaatgtct atgatgttaa ttctaaagga ttaactgaac
    22621 tcgctctaag atttaatcaa ggtattaaca ctgacggtga tgcgctgaag aaacgtacac
    22681 acttcaacat cgctggcgcc tttaacccta atgttcgtaa attatatggt gccgtcaaaa
    22741 gattagagaa aaagatagaa agcggaatgt cttattttat aacacaaccc gtgtacagca
    22801 aagagaaaat cattgaaatt taccatgcca ctaagcactt gaacaaacca tttttcatag
    22861 gcattatgcc tatcgcaagt tacaaaaacg cactcttttt gcataatgaa gtgccaggaa
    22921 tcaagatgtc agatgaaatt ttacaacaat ttgaagcagt taaagatgat aaagccaaaa
    22981 cacgagaact aagtcttaag ctttcaaagg atttaatcga tactgttcat gaatatttta
    23041 atggtttata cattatcaca ccgtttcaaa atgtcgaaga ttcattagaa cttgcagcat
    23101 actcaaaatc tattactgct cacaaggagg caatattatg acaacaatta aaacatcaaa
    23161 cttaggattc ccaagattag gtagaaaaag agaatggaaa aaagccatcg aaagttattg
    23221 ggcaaagaag atttctaaag aagaattaga tcaaacatta actgatctac ataaagaaaa
    23281 tttattatta caaaagtact atcacttaga tagcatccca gttggtgatt tctctttata
    23341 tgaccatata ttagatacgt cattattatt caacatcatc cctgaacgtt tccaaggaag
    23401 aactattgat gatgatttat tatttgatat tgctcgcggt aataaagacc atgttgcaag
    23461 tgcacttata aaatggttta acactaacta tcattacatc gtacctgaat gggacaatgt
    23521 tgagcctaaa gtgagtcgca atgtattatt agatcgcttt aaatatgccc aatctttaaa
    23581 cgttaacgct caccctgtca ttgttggtcc gattactttt gttaaattat caaaaggcgg
    23641 tcaccaaact tttgaagaaa aagttaaaac gttattacca ctttataaag aagtgtttga
    23701 atcattaatt gatgcaggtg ctgagtacat tcaagttgat gagccaatct tagttacaga
    23761 cgacagcgaa agctatgaaa atattacacg tgaagcttat gactatttcg aaaaagctgg
    23821 tgttgctaaa aaattagtca ttcaaacata ctttgaacgt gcacatctta aattcttaag
    23881 ttctttacct gttggtggta taggtttaga ctttgtccat gataatggct ataaccttaa
    23941 acaaattgaa gctggagatt ttgataaatc aaaaacatta tacgctggaa ttattgatgg
    24001 tcgtaatgta tgggcaagtg acattgaagc taaaaaagtc ttaatcgata aattgttagc
    24061 acacactaat gaacttgtca ttcaaccatc atcttcatta ttacatgttc cagtatcttt
    24121 agatgatgaa acattagata caagtgttgg cgaaggctta agctttgcaa ctgaaaaatt
    24181 agacgaatta gatgcattgc gtcgcctatt taatcaaaac gatagtgtta aatatgataa
    24241 attaaaagca cgttatgagc gtttccaaaa tcaatcattc aaaaacttag attatgattt
    24301 cgaaagcgtg cgtacttcaa gacaatcacc attcgcgcaa cgtattgaac aacaacaaaa
    24361 acgtttgaac ttaccggatt taccaacaac aactattgga tcattcccac aaagccgaga
    24421 agttcgaaaa taccgtgcag attggaagaa caaacgcatt acagacgaag catatgaaac
    24481 attcttaaaa aatgaaattg ctcgatggat taaaattcaa gaagacattg gcttagatgt
    24541 attagttcac ggtgaatttg aacgtaatga catggttgaa ttcttcggag aaaaattaca
    24601 aggtttctta gtaactaaat tcggttgggt gcaatcatat ggttcacgtg ccgtaaaacc
    24661 accaatcatt tatggtgatg taaaatggac agcgccttta actgttgatg aaacagttta
    24721 tgcacaaagc ttaacagata aaccagttaa aggtatgtta actggacctg taacaattct
    24781 aaactggtca tttgaacgtg ttgatttacc acgtaaagtc gttcaagatc aaattgcttt
    24841 agcaatcaac gaagaagtat tagcacttga agctgcagga atcaaagtta tccaagttga
    24901 cgaacctgca ttacgtgaag gcttaccatt acgctctgaa tatcacgaac aatatcttaa
    24961 agatgctgtt ttatcattta aacttgcaac gtcttcagtt cgtgatgaaa ctcaaatcca
    25021 tacacatatg tgttattctc aattcggtca aatcattcat gctattcatg acttagatgc
    25081 tgatgttatt tcaattgaaa catctcgtag ccatggtgat ttaattaaag actttgaaga
    25141 tattaattat gatttaggta ttggtctagg tgtatatgat attcatagtc cacgtattcc
    25201 aacaaaagaa gaaattacta cagcaatcaa tcgttcacta caacaaatcg accgctcatt
    25261 attctgggta aaccctgact gtggtttaaa aacgcgaaaa gaagaagaag ttaaagatgc
    25321 attgactgtg cttgtgaatg ctgttaaagc taaacgccaa gaataatcgc agcatttatc
    25381 atataataaa ctaaaaacgg gtgattttat gacacaatat cctttatggc aacaattaaa
    25441 tactttaaaa caagcaactt gggttgattt aacgcataca tttgatccgg aaataccacg
    25501 ttttagtgag tttgaaaaag gcgaagtttc aactttattc acggttaaag atcatggttt
    25561 ctatgtgcaa cgttggaata ttgtaacgca atatggtact cacattgatg caccaattca
    25621 ctttgtcgag aataaacgtt atttagaaga cattgattta aaagaattgg tattgccgtt
    25681 aattgtatta gacttttcaa cagaagtagc gaacaacaat gatttcatcg taacacgtgc
    25741 gcatatagaa gcatgggaaa aagagcatgg tactattgaa cctggcactt ttgttgcact
    25801 tcgtaccgat tggtcaaaac gttggcctaa tattgaaaag tttgaaaaca aagatgcgaa
    25861 cggacaacaa catgcaccgg gttggggatt agatgctttg aagtacttaa ttgaagaacg
    25921 tcgtgttgaa gctgttggtc atgaaacatt cgataccgat gcatctgtgg acgttgtaaa
    25981 aaatggtgat ttagttggcg aacgttatat tcttggtcag gataaatacc aagtcgagtt
    26041 attaactaat ttagatcaat taccaactcg tggcgccatc atttacgcta tcagccctaa
    26101 accaaaagat gcacctgggt tccctgttag agcatttgca attaaaccat caaatgacta
    26161 atattaccca ataaatccat aaacaaataa aaacaggtta tgccaacatg taattaatac
    26221 tgttgtgata acctgttttc ttattattta tactttgata caactgcagc gatagcttga
    26281 ccgccaccaa tacacaatga tgttaaacca gtttcaactt catcattcag ttgatgcaat
    26341 aatgtcacta atactctagc accagatgca ccaataggat gtcctaatgc aatagcgcca
    26401 cctttaacat tcaccttttc aggaggtaat tttaattcac gatcaacagc taatgattgt
    26461 gctgcaaatg cttcatttaa ttcaaataca tcaatatcgc ttaattcttt tttactacgt
    26521 ttcaaagcct tttctacagc gccaactggt gcaataccca taatagaagg atctacacca
    26581 tgacttccaa agccatcaag cactgccaat ggttcgatat ttaattcttt agctttgtct
    26641 tctgacatga ctaacatcat cgcagcacca tcattgattc ctgatgcatt acctgctgta
    26701 actgtaccgt cttttttgaa agctggtctt aatcgactta atttttcgac tgatacattt
    26761 tcacgtacac cttcatcctt agtgactacg attggttcac ctttacgttg aggaatcgat
    26821 actggaacta tttcactatc aaattcacca ttttgctgtg cacgtactgc tttttgttgt
    26881 gagtttacag caaatgtatc ttgttcttct cttgaaatac catattgctc tactaaattt
    26941 tcagcagtaa tacccatatg atattgatta aatacatctg ttaaaccatc atataccatg
    27001 ctatcaacca ttgattgatg tcccatttta aaaccaaagc gactgttgtt gacaagcatt
    27061 ggtgattgag acatattctc cataccgcca gctagcacga tgtcattttc accagtcaca
    27121 atagattgat atgctaattg aatcgacttt aacccagaac cacatacttt attcaccgta
    27181 aatgcaggta ctgtttctgg caagccacct ttcatagcag caattcgtgc tggattttgt
    27241 ccttgtcctg cttgtagtac gttaccgatg ataacttcat caatctcact tggattcaaa
    27301 cccgtctctt taataatatg ttctattaaa gtcgcaccta aatcataggc tggcacgtct
    27361 ttaaacgcac ctccaaaaac gccaataggt gtcctgtatg ctgctgctaa tacgactctc
    27421 gtcatgtcat attcatctcc ttatgatata tacaatttta ttactaatac gtctaatgaa
    27481 ccaatacgcc actaacatcc aatttatgaa accaattgct acgtatgtta cattttcatt
    27541 cttgttatgc atttaacaaa tcatattata caatgatgta atttgtttgg gcaatcggaa
    27601 acgtaaacgg tttcacttta acatttcacc ctcttaaata tgctctatca tcctcatcta
    27661 tagtacaaaa ttatgctttg atttaccaca aattatcatt aatcatttcg cgtaatgact
    27721 ctaattaaat tctatctatc attgcatcgc tattcacagc tggtttcacc atcaatttct
    27781 catcaatttt ttattaccat gacgtctcca gtcaaatatt aaatcgctga tgattgttaa
    27841 ttccgcaatt accttgcagt cacctctcct tccacttcat cgccaaatct gcccctattc
    27901 actagaaaaa ataaatagta tacttagtaa attaattaca catcttaaag gagttaaaat
    27961 tttatggtta atgcattaac accaaaacat ttagctgaaa agcgtgcagg attccatgag
    28021 ttgttctttg atttaatttt cgtctatgcc attcaaaaaa ttgcacatgt gattttaaca
    28081 acacaaaacg gttccatttc agctgattta tttttcaaat acatcgtcat gagtttgttc
    28141 ttatggttaa tgtggtctca tcaaacattt tttacaaatc gatttggtca agttacattc
    28201 aaagacgtct cgtttatgat gtttaatatg ttcatcatgg tcttcctatc taacagccta
    28261 tatccggact ttgaaaagac attcttccca ttcttcttat gcgtggcaat tatgtattta
    28321 agtatcggat tacagtattt attacatata cgtacagggt tagattatgg cgataagcgt
    28381 acatgtcaag cttttgcgag cgtagccttt gtgatttcat ttttatcatt tctatcatta
    28441 gtactaccac aaagtatcca ttacatccct ggatttttag gcgtattcat tgcagcgaca
    28501 ggacttattc catttcagaa atatcttgta ttatcacctg ttaatatgat gcatttagta
    28561 gaacgttttt cgttactaac aattattatc tttggcgagg tactggttgg tttagcttcg
    28621 tcatcattta gcattgatca ttttagttac atttatatat tccaatttat gattttaatt
    28681 agtcttttcg gtgtatattg gatcattact gaaaattata ttaatcataa attaacatct
    28741 atcggtttcc gtttatcgta tacgcatcta ttaatcaata tcgctttagg tgtgattaat
    28801 gctgcaattg tttttagtaa taacaataaa ctaaatgact tgtttgaaat taatatgatg
    28861 tatatcagtg ttttaatatt ctatattgga ttatggttaa tcacgcctta tttccataat
    28921 gaactgacaa acgcgaagta tatttcatat tcactaggta tactcgttat gtcttatatt
    28981 gtcagccttt tatttaaagg tcacgaccat gtaatgatta tctcagtatc agttgcaacc
    29041 ttctgcatca tgctgattta ttttaaaaat caacgtctac gtaataaaca tgaataaata
    29101 gtatttacct cggtcaaagt attggaactg aatccaaaag tgtcgaactt cacattatac
    29161 ttttgtgaca aacttcagtt atgctacatg atcgaggttt ttaaatattt ccgaggaaat
    29221 cgttagccta tacaatcatc aaatgatact tataaataac atatatcctc taatccccac
    29281 tatctaaaag gaaataattc ccacacaaca taaccacctc aaaacaaata aatcaatata
    29341 gatatgtcac ctatacttat cccccacatt tcattccaca ccaaaaagaa cagatgacaa
    29401 tcgtaaagat ttatcacctg ctctaaatat tatttcaaat actatcatca ccaaaatcga
    29461 caattatagt tcttgtttgt taaaaatttt aatagacaat atataagaaa cgctcgttat
    29521 aactatacca atgaccgcat atgctatgac aactagccat ccttcagtgc taattttttc
    29581 aaattctaat gcagatgcac cactaaccat accatatata aagaaaaaga ccacgaaaga
    29641 aataagtagt agtatagatg cacatatcat aatgacattg gagttttcag cgccaaattt
    29701 aaatgtcaat gggaaaatga tagagtatgc ccctatgata ccaaaactca ttaaacctga
    29761 cataatacca atcatcacac tttgggtcac aatcgtagtc acaactaatc caatcattaa
    29821 acttgcaccg aataagatta aataaaaggc aaagtatgac ttaatataat cactacgttt
    29881 aaccggtaaa gtagatacat aatacatcca tcttgagtct ttttcatgtt taatattatc
    29941 agtaataggt gtgattaaca taaccccagc cattgccgaa ctcatcaacg gattaaatac
    30001 tgcaaagtat cctgcagcta taatagcgac tataaaataa atatatgttt gctttctcgt
    30061 tgcataaaaa ctacttagga acataccttt cattatactt cacctcgcat tatgattttt
    30121 gtagcgtcat caatgtgttt taaaggctct gcattttcaa tttctgcata atctttaact
    30181 aaaatttgat atttcccctt tgacaatcta gaagcaatga ttaaatgctt aggaatttca
    30241 acatctttat cttctgtcgt aacaattcca tattgtgcta aaagtatatc tttctgttca
    30301 gttaaaatca ttcgtccatc tttcataaac actaatttat cagctaaatg ttctatatct
    30361 tcagaaatat gcgatgatat taagatgcct ccaccttgtg cgacaaaatc ttctagtatt
    30421 tccattactt cttctcttcc agaaacatcc atacctgcag ttgcttcatc taagattaat
    30481 aacttcacat catgagaaag cgcaatcgtt aaagctatct tcattcgcat ccctcttgaa
    30541 aaagttttaa ttttagtttg tagtggtaac tcgaaatatt tgattaaatc aaagaatttt
    30601 tgactattcc aagtcatata aatagattga aatactttat caatatcttt aatagtcaat
    30661 ttattaggca ctctcaaatc atcaaacact acacctatgt gttctttata ttcgacatca
    30721 tccacggtat gttcttctcc aaaaaatgtg atactaccgt tatctttatg tcgattgcct
    30781 actagaatat taattaatgt cgatttacct gagccatttt tcccaattaa tccaactgtc
    30841 tcattagtag aaatcgttaa agatgcatct tgcaatttaa aatccgaatc tttgtattgt
    30901 ttgttcacac tatctacgtt taacaatgtt gtcatatcca tgctcctctt tgtttaattt
    30961 taataaaaac gtcttctctt caataagtaa ctaaaggcgt tatatatgaa aatagcaatg
    31021 ataagtagca aactaaagct ttgattaatt ccagtagtaa ttgaataaag cccaattact
    31081 acaatggcta gtatcaatat tgataagttg atcgcatatg ttttaaataa tgcaattaat
    31141 tctatatggc gttcaccttc atccaatatt tccaatcgct tttcagtgta attcttatct
    31201 gcaatttttg gtattctgtc atcaaacctt ctattaaaca gtgcaaattg tgtattgaaa
    31261 atagcactag caaaaaatgg tattaaaaag aatagtattg catttgctgc agcgtgccct
    31321 accacaaaaa taagcaatac taaaaaagca ataactgtct gcagaatact taatatactt
    31381 ccatttaaaa cataacgatt cgcaagcaac tcatattgat ctgttgcatc gatatctacc
    31441 tctttatcta caagttgctt atacttcaat gcacgtcttt gattcatcaa aacaatggct
    31501 tctaccaata tgataataat cgtcgcaaca atcgatatta caacgacatt gttatatgtc
    31561 gcaaacgtca agttctcaat accaaatcca tcaaaattac ctaaaattcc acctatgata
    31621 ccccctacaa gtccacccag taaaagatat ccgatatatc ttagtatttt catgcctcat
    31681 cctcctcaat aataaaaaca gtttccaccg tttcattgaa aatgcgagca atttttattg
    31741 ccgttaatac tgatggcata aaattgtttc gctcaattag cgatatggtt tgtcttgaaa
    31801 cgcccgcttg tttagcaagt tgcgtttggt ttaagccatc tcgtgctcgt aattctttca
    31861 atcgattacg cacatcgcat caactcctta attacactgt aaatgatatg cgtctttttg
    31921 acaactatat ttgtcaaatc tacaccaaaa aatatgatta tccacctatg tatgacattt
    31981 tgaaacaaac acctcaacgc ctacaagtca taattgttta ctttcgttac accttcctgc
    32041 ataattaaca gcattctaat tttagtatga tgcacgcatt ttcactaaat caaaccattc
    32101 aaaggagact attatggcat ttacattatc tgcaattcaa caagcacatc aacaatttac
    32161 tggtgttgac tttccaaaac tattcaaagc ttttaaagat atggggatga cttacaatat
    32221 cgtcaacatt caagatggca ctgcaacata cgtacatcaa tcagaagatg atatcgttac
    32281 gtcatctgta aaaagtaatc atcctgttgc tcaaaaatca aacaaaacaa tagttcaaga
    32341 cgtcttaact agacatcaac aagggcaaac agattttgaa acattttgtg atgaaatggc
    32401 tgaagctggc atttataaat ggcatatcga tattcaagcg ggcacttgta cttatatcga
    32461 cttgcaagac caagctgtta tttcagaatt aatccctcaa taaactatat ttatagcaac
    32521 attttaatta tttcataaaa ttttattgat aatcattatc gttcggtata aagtaaatac
    32581 tatatactac ttatgagtga ggttgattat catgataact aacactttta ttttaggcat
    32641 cacaggccca acaagtcttg tcgtcattag cattatcgct ttaattattt ttggtccgaa
    32701 aaaattacca caatttggcc gtgccatcgg ttctacttta aaagaattta aatctgcaac
    32761 agaagattta gataaagagt ctcacgatac acccagtaag gaatcgaaac aacagcgaga
    32821 gcaatagcac tgaccacacc ttactggttc actttagcga actacgccat cggttagtaa
    32881 aaattttatt gtcgttcgtc attacggtca tcgtcgtata tgtttcatca ttttggtgga
    32941 tgacaccatt cataacgtat attacccgtg cacatgtgtc cttacatgca ttttcattca
    33001 cagaaatgat acaaatatac gtgatgatta tatttttcat cgcgttttgt ttcatttcac
    33061 cagtcatgtt ttatcaattg tgggcattca ttgcacctgg attacataat aatgaacgtc
    33121 aatttattta taaatatagt tttttcagcg tgttattatt ctgtgctggc gtcgcttttg
    33181 cattttatgt tggctttcca atgatcattc aattcgcgtt aaaattatca ttaactttaa
    33241 acatttcgcc tgtcattggc tttaaagcat atttgattga actcatacgt tggttattca
    33301 catttggtct tctatttcaa ttaccaatat tatttatggg tcttgcaaaa ttcggtctta
    33361 tagataccac atcattgaaa cattatcgta aatatattta tttcgcttgc tttgtattag
    33421 caagtatcat tgcaccaccc gatttaacat taaatatatt actcacattg ccactcatat
    33481 tgctctttga gttcagtatg ttcatcgtta agttcacatg ccgaggcaaa ccgccaacac
    33541 attaacctta tacgaatcaa tgctgtgatt atcgttatac tgcaataccg aatatttcca
    33601 atatagcaac aaaaaaacag gcactcggcc tgttttttat tttttaaatt taaagattaa
    33661 gatagcaact accataatat aagcaccttg tgctatcaat ggttcataac ttggataaaa
    33721 gcccaaccag ttaatcgttg ggaatccttc aataacatgt cttggcatcg cacctaataa
    33781 ttgtaacttt tgaatactta cgccaagcat tttgaatccc ataataaaaa taaagatcga
    33841 caacactcta aatatataga aaataggtat taatttaact ataaatctaa ataataatgc
    33901 aaagatgatt aaaataacga tagctaaagc aataccaata ataaaatctt tggtcgctag
    33961 ctcacctatc atccccatat agaaaatgat aacctcgaca ccttcacgca acacagatat
    34021 taaaccaatc gtcgctaaca ataccaaatt accattacta atcgcattag catacatatt
    34081 tttaatcatg tcattccaac gttttgcatt tgaacgtttg tgcatccaaa caccaacgat
    34141 aaacattaat atgaccgcaa cgatacctaa tcccgcttcc atactttcac gaagaatgcc
    34201 actattccct aaagtttcta caaacgtaat tgctaagata atactcagta caagtccggc
    34261 aattgcacca ccaatcacac ttgcagtccc tttcttatct tttacattac gcgtcatggt
    34321 agtcaatgtc attacaatta acaacacttc tagcccttca cgtaaaaaga taatcatcac
    34381 atcgacgaag ctataactat ggccaacaac ctctttaatt tggttattta aatctactaa
    34441 accatctttc acatgtgctt tattatgttc gtctaataca ctttgataat atggtatttt
    34501 atcttcaatt ttcgtataca aagcaccgtc tttagtttga atttgacctt caacatacgg
    34561 ccaagtttct ataaaatgtg taagcgcagc atcagcatcc gacaattgat tgtcgtcgat
    34621 agctttaatc gccttctcta acgcatcatt taattgtgat acatggtatt gatcatttgc
    34681 agacgtatta ctttttttat cgacatgatc aatatttgat ttaaaagttg tccaagcatg
    34741 tgacactttt gccgtatcta atggtgactt atgaattgca attctaagtt gtaataatgc
    34801 gacttcaatt tgtccatatt gatttgcgtc ataattgcga atcactgttt cattacttgt
    34861 ccaaatctga ttcaaactat tgttcaaaga ttctaattcc gctttatttt tatctttaat
    34921 cgcttttgtc atcgcagcat ctttagcatc gacttgctgt tgcaatagtt taattttaga
    34981 acccgcatct ttactagcca atttctcttc ataagcaatt aatgacttcg ttaattgcga
    35041 aagtgtatct ttttgattat cattcgcttt tgcatcttca agctttctca catctgattt
    35101 gacagcatta ctttcactat tatcttcaag cgataatttc ttaactgcac ttaccacttg
    35161 ctcaattgct ttctgcttat tgtcattcga tatcgaatta ttagaaagtg cagatttcgc
    35221 atccgttatc acactatata catcactaat actttgttgt tctgctgcct gacttttcag
    35281 taacccaaag ctacacacca tagcagcagt tattagcatt gctacaaatt tagtcaaata
    35341 atgtttcacc aaggtatcct cccttactaa cacctggtaa tactaaaaat gaagcagaac
    35401 ctctatgtgt aatatattca tttaatttat cattactacc taaattattt tgtatatcga
    35461 taaattgttt tgtcgctttt tgaaaagcaa taaaaagtaa gcctgtttcg aagttacctg
    35521 tgcggtcatc cgtaccatcc acataattaa aggctctacg taaaattgac gtatttgctt
    35581 ctttcgctag cctcgtatgg gcatctttat caataatata ctcgccatga ctatctttcg
    35641 cttttaagtc aatttcatca aactctttcc cacctgttaa cggtgcacca ctatgtcgtt
    35701 tccgaccaaa tgtagcctct tgttcttcca gcgcagtacg atcccacgtt tcaatgtgta
    35761 tttgaatacg tctgacaacg caataagttc catgtttcgc ccatccgtca tcaataaaca
    35821 cataatcttt aagttgatta ttcttcctag gattaattgt tccatcttta aatgccatta
    35881 aatttctagg tgtttcctta cctttagcag agataaaacc agattgcgcc caacgtacct
    35941 taacaatatc tcgaaatgga cgaactaaat tatgaaccgc atgaaaggat acttgcgaat
    36001 catttgagca tgcttgaatc ataatatcac catcgctgta atcgtctatt aactgatcat
    36061 tcggaaaatg cggtaaatct ttaaaggcat cgggaatttt actagataac ccaattttct
    36121 tcatcaaaga cttactaatc ccaaaggtaa tcgttaactt gcttgcacct aatcctatcg
    36181 attcaccggt atctactggt ggcattaaag gattgttact cggcttgcca actgtatcac
    36241 catccatcat acgatcagcc ataaccgtcc actttttaaa cattgcctta attgcatctc
    36301 tatcttttga cttcaaatct aacgcaacga aattgcatgt tttttgcgtg ggtgtggtaa
    36361 tgcctggttg cactttacca taaaattcat acgcatcttt ttccggatct tctggtgtat
    36421 tgaacattga tttgaaagac cacatgctac caacaccact tgcgccaatt gcaacaccgg
    36481 caccgccaat acctaacatt ttcaaaaatg tacgtcttga aaattgcgta ctatcgttaa
    36541 cttgttcata atttgtcatt cagtaatcac agccatttta cttagtggct ctcctaatgc
    36601 attaacagca tccgcaaatg ctttacggtc tttcttcgat actttttcaa atgactcata
    36661 accgccgttg ttatctttat atttatccaa taattgattc actttatcga agttcatttg
    36721 gatatcatca cttaattttt tatctttttt ctctaaaata ggtttaaata agtcataaat
    36781 tttttgtgcg ccttcaacgt tcgctttaaa atcatataaa tctgtatgtg aataaatttc
    36841 ttcttcacct gtgattttag aagttgcaac ttcatttaat aggtcaacag aaccttgtaa
    36901 cattaatttt ggtatgatat ctaatgtatc agctttggca tgcaattctt ttgcatcttt
    36961 caataattgt tgtgcatctt ttttagtcac atcatcaatt ttcttatctt cgtataatgc
    37021 tttttcaatc ttatgatatc ctgaccattc tttttccttt ttctcttctt tcatatctgc
    37081 aagacgtgca tcaattttag gatctaaatc tccaaatgct tctgcaactg gttcagagcg
    37141 ttcataatac atacgaactt ttggatataa cgcttttgct tgggccatat cattattttc
    37201 aatcgcttta acaaattttt ctgtaccttt taaaaattca tttaactgtt catcagtata
    37261 tttatcatat gcttttgttg cttgttttaa ttctgcttta acaccatcat cttttttcga
    37321 tgtctttgaa tcatccttct tactatcgtc gttcccacat gcagcaatta ataacgttga
    37381 tgctaataat agcgttgtta actttttcat tttgacacct cattatagaa attctattat
    37441 ccccatagat gtgttcttat aaatattatt ttcgcattcc ctacacgcta aaataataca
    37501 ttacacttaa aacggctgtt ttaaagcatc ctcccataaa catcatctag ttgataataa
    37561 ttatcaaaat cattatattg ttataaaata atactgtcaa caacctttta tttttaaaaa
    37621 ttaaataaaa gagcgctgcc attttaaatg tcatcactct tttgtaagtc taatttgtca
    37681 tttttggtcg tattctgatt ttaataaact atagatgtaa ctcgatgaat aaataccatt
    37741 tagtaattca ttgtcacgta acataccttc tctagtaaat cccagccttt caggaatagc
    37801 ccggcttttt tcattattaa ctgcggcact aatctcaatt cggtttaagt caagctcttc
    37861 gaaacaatac tttatcaatg cctcaactgc ttgtgtcata atcccatgac cctcaaattc
    37921 tttatctaaa tagtacccta atgaagtttt tctgtgcatg tgattaattt catgtaaacc
    37981 gatgacacca actagcgttc cttcatacca aatgccacac tgaaatccat taccatcagc
    38041 aaattgcaaa agtcctcttt taataaatgc acgcgtatct gatggttgct cagttgcatc
    38101 tacccaaggt aaccattccc taagtgaatt tcttgaacga ttgactaaat tgaaaagcgc
    38161 ttctgtgtca tgagcttcta aaatttttaa tgttatttgt tcattcactt tcattccaaa
    38221 cataccatca catcctcatt catttttcat ataattctgt aaattatcat tataataaca
    38281 tattaatgtt acgcattgta tcttaaattt gttgtccttc cccaacttgc attgcttgta
    38341 gaatttcttt tcgaaattct ctatgttggg gccccgccaa cttgcattgt ctgtagaaat
    38401 tacggaccca atttctctat gttggggccc atccccaact tgcacattat tgtaagctga
    38461 cttttcgtca gcttctttgt tggggccccg ccaataagcg ttgtagtgcc tattactttg
    38521 attgatgtcc aagtttccta tcacacaact taatttaatc actactttat tattcattcc
    38581 aaatttatat ttaaaaatac gacaaaacag tgctaaaatg ctattaaagg aggcactgct
    38641 catggcatca ttgaaaaata agcattttat gattgggcta tcactaacat ttatagtcgc
    38701 attatttagc tttttagcag ctaaattgcc aatcctagat aaggttggcg cattaactat
    38761 cgcgatatta atcgctattc tatatcgtca ctttagagga tatcctgaac aatatagctc
    38821 aggtattacg ttttcatcta aatatttatt aagatttgca atcatcctat atggacttaa
    38881 actcaatata ttcgatatta tcggtcaagg tagtaaacta ttagccatcg atgtaggtgt
    38941 cgttattttc agtattgtaa tgatgctctt tgtcaacaaa ctgttgcatg gtgacaaaaa
    39001 catcgcatta ttactaggtg tcggcacagg cgtttgtggt gctgctgcta ttgccgcagt
    39061 cgctccaata ttcaaatcac gtgaaaagga tacagctatt agtatcggta tcatcgcatt
    39121 gattggtacg atattttcac ttatatatac agctatctac gctatctttt caatgacgac
    39181 aaatgtttat ggcgcttggt ctggtgttag tcttcatgaa attgcacacg ttgtcttagc
    39241 tggtggcttt ggtggtagtg atgcacttaa aattgcactt cttggtaaac ttggtagagt
    39301 attcttactg attccattaa ccatcgtact tattttaatt atgcgtttcc gttcatcaga
    39361 atcatctagc aagggtcgaa taagcattcc atacttttta attggatttg tcattatggc
    39421 cttagtgaat acatatgtaa ctattccgtc agcattgcta aatattttaa atactgtatc
    39481 aacaatttgc ttattaatgg ccatggttgc tttagggtta aatgttgcat ttaaagatct
    39541 taaaaatcgc gcacttaaac cgcttatgac aatcattatt acctcaatat gcttgtcatc
    39601 acttgccttt atcgtcgttc attggctata tagttaatat gaaaagacgc tcaaacactt
    39661 acaaatttag tgtgccgagc gtcttatttt tatactttaa aagtcgtatt ggttgttttc
    39721 ttcttcaaac acttgttgtt gtactttggc agttcgcatt gtggattgtg ccaacgcttt
    39781 taaccgaaat acaacatcat cattgacaat ttgttgattt gaaaaatctt tctcttcaat
    39841 aaatacatac gtttgcatcg tatgtgcttt catgtaactc aatattggtt ttaaatgcat
    39901 ttcaggaatt aaataatgtt tactagaacc tgctgtcgct acaagtccta ttaccttgtc
    39961 acgaaacgca ttgactggaa gtagatcaaa cacatttttc aaagcaccag ggatggaagc
    40021 ttgaaaaatt ggaaaaccaa taaaaatcac atcagcctgc attaacgacg tcgttaattt
    40081 atatacatct cctgtagtat ctagataatt ctttccaacg ctaaattcta attcaagttc
    40141 tcgtaaatcc atcaactcta tttgatgtcc ctcattgatg acttctagtt cattttttaa
    40201 atcatccata gcaattctcg ttttagaacc tactgtggaa cctgacaata atacaatatt
    40261 catgatgacg cccctcctat tttgataaat gctttttaat cgccggtata atgtcgttgc
    40321 caattaactc aatattcttc ataacatttt caaatggcac accgccaaaa tcaagctgtg
    40381 ccataaaacg ttgatgacca tacaactcgt gttgatacaa tatcttttca ataatttgtt
    40441 gcgggcttcc aaccattagc gcttctcgat aatctgacga attagcaaat tgctgtttcg
    40501 gataaccaac accacgaata aatgacatcc ctgtattcaa atgtggataa aattctctca
    40561 tagcatcctg agttgtttca gctgtataaa acagactcgc tgtacttact ggtaaagact
    40621 taggcgaagc atcgaaacct gcttcagttg ccgcttgacg ataagcatct atagaacctt
    40681 taaagttcat tgctgggcca ccaagggttg taatcatcat tggcacacct tgttttcccg
    40741 ctttaatagc acttgcaggt ggaccaccaa cagcacgcca tattggcaat atattatcga
    40801 ttggtcttgg gaatattttc atatttctaa gttctggacg atattttcca gaccaagtaa
    40861 tacgctcagt tttatttaac tctaaaagta aacctaattt ttcttcaaac aattcatcat
    40921 agtcttttaa atcatagcca aacaagtcaa aaatacctgt tcttgatgct ctgccagcta
    40981 caatttcggc tctaccatga gaaatcaaat ctaatgtcgc gaagtcttca aatactctta
    41041 caggatctgt tgcactaata atcgttgaag aacttgaaac tttaatatgc tttgtcgctt
    41101 gggcaattgc acctaacaca accgtatgtg cctgtgttgt aaaatgctcc tgatgacttt
    41161 caccaactgc aaaaacatca atacctgctt catctgctaa tttacttgct tcaataattt
    41221 cattaatacg ttgctcataa ctaacttttt cacctttcaa tggattcaat aaatgatcac
    41281 ctaaggaata caaaccaaac tcaagaggcg tatttttatt catttctaat ttggccatta
    41341 aacattctcc tttatatttc gtgtaactta ctttcaattt gttcacgttc tgcttctaaa
    41401 aagtcaggca agttcaattg acttcctaat tgttcaacag gtgtatcaat agtaaatcca
    41461 ggcgcttcag tcgcaaattc atacataatt gaattatggc gatagtataa tgatttaaag
    41521 aaatagcgat caattatacc tgaattactt tgtggttgtt gttgtaattt cttgtaaatt
    41581 gcatctaagt cactcatttg tggtgtatta actgcaatat gatggatata acctcgtcca
    41641 ggtctttcac gttgtccctg ttgctcaata actacaaaat ccgaatataa accttgtggt
    41701 gctaatgtca gcacatcgaa tgatttatta tctcttttgc gataacctaa gacattttcc
    41761 aaaaattcta ccgtacgcgc tgcatttcta actcttaatt cgactggccc tattcctaaa
    41821 atttgatatg cttgaggtat ttcactataa gcgttatgtt gccattgatg tggtacttca
    41881 aaactatcat taacaagcaa tatgatttct aagtcgtctt catctttaaa gacaacacct
    41941 tcttgtccaa gatagtacaa acgttcagac ttaataccat tattctcaag acgtttttca
    42001 aaataatgaa gtgccgcttg atttggtact aataatgaaa atcgataaat cgtttcagta
    42061 cctggctgct tatgacccgc attgggaatt tcaaaaaagc ttaaaattgt tccggctgta
    42121 cctacttcgt ccccataaaa caaatgatac attgaaggat tgtcttgatt aaccgacttt
    42181 tcaactaatc gtaatccaag gacatttgtg taaaaatcct tattacgttt tgcatctttt
    42241 gtatacatgg atatgtgatg atgccctact atattcatac tatccctcct attagtataa
    42301 attttatacc agcttacata aaaaagatag ttatatgcca tttacccctt gcaaataaaa
    42361 atatgtttgt actcattcgt agatacacag ctagtatgta taacgacata ataattgcat
    42421 aattataaat ttatgtgtag caatttattt tttctaaatg tttataaaat acttcaataa
    42481 ctaaatatgc aagatttcac aaaagcatat tgacaacgct ttcatacatg tctaatataa
    42541 gtttatcgca caaaatgcat ataaatacat attaaggagg attttatgaa ttttcttaaa
    42601 cctgcaaagc atattaagcc tttgccagaa aatcagatag atgataccta taaacgatta
    42661 cgtctccaag tatttcttgg tattttcatc ggttatgctg ggtactattt attacgtaaa
    42721 aacttttcgt tagcgatgcc ggcattgcaa gagcaaggtt ttacaaaagc ggaactaggt
    42781 tttgcacttt ctgctgtttc catcgcatat ggatttagta agttctttat gggtactgta
    42841 agtgatcgga gcaatgctcg gatattctta gttcttggat tagcactcac tgctatcgtc
    42901 aatttgttaa tgggatttgt accgttcttc acatcaggta tcggtattat gtttgtccta
    42961 ttattcttaa atggatggtt tcaaggtatg ggctggccac cttcaggccg tgttctcgtt
    43021 cactggttta gtgtaagtga acgcggaagt aagactgccc tttggaacgt tgcgcataat
    43081 gttggtggag gtattatggc acctattgct gcttggggta ttacaacaac tgcatttatc
    43141 aactttggtt atttaaaagg tttcgaaggt gtattcattt accctgcact cttagcactt
    43201 atcattgccg caatttcata cgtattgatt agagacacac ctcaatctca aggtttacct
    43261 ccaatcgaaa tttataaaaa tgactttgct acaagcgata agaaaacatt agaaacagaa
    43321 ttaactacaa aagaaatttt atttaaatat gtactgaaca ataaatgggt atgggcaatt
    43381 gcctttgcaa atatatttgt ttatttcgtg cgttatggtg tacttgattg ggcgccagtc
    43441 tacttaagtg aagaaaaaca tttcgactta aaagcatcag gttgggcata cttcttatac
    43501 gaatgggctg gaattcctgg tacattatta tgtggttaca tttctgataa attattcaaa
    43561 ggtcgtcgtg gacctgcagg tttcttcttt atgttaggtg tcacagtatt tgtattaatt
    43621 tattggttaa atcctccagg caatgcttgg ttagacaatg tctcattaat tgccattggt
    43681 ttcttaatat atggaccagt tatgttaatt ggtttacaag cattagatta tgtacctaaa
    43741 aaagcagctg gcacagcagc tggattaaca ggattatttg gttatctgtt tggtgctgta
    43801 atggccaaca tcgtcttagg tgctgtagtt gataaattcg gatgggatgt cggttttatt
    43861 ttattaacag caattagtgt gtttgcaatg ttgagcttta tcctcacttg gaataaagta
    43921 ggacaagaaa ccgttcatca ttaaatgata aaaaataaag tcatatggtt atcttatcga
    43981 aagatgatat attcatctct tataagttca accatatgac tttttattag tattcaaaaa
    44041 aatatttaca ttgccacttt tgtgtttgcc ctgctgtttt attcaattga ttacaccact
    44101 taggataaac tctaaaagcc attttccctt gatacttaga tgaagctaag ataccttttt
    44161 ctaccaataa ttccctagga aatagaaagt agccatttaa ttcatcatcg ataacagcaa
    44221 caatcaggta atcagcaaac tcttcaattt tgtatggccg attataatta tcttcgtctt
    44281 ttgtccaaca tgtcacgaaa tatcccgatt tagtcggtgt tttcttagct aacctacttt
    44341 gatatgtttc ctctttaaag ctaaatgtta atgcttcgta atcttgatta tatttttctt
    44401 cagttaaatc tttaacttct gattcttcac taaaaatatt tttcaacagt attttagatt
    44461 tacacatatg ccaatctcac tttatttttt cataatcgta tcatatattt atttttttcg
    44521 aaaaatacac ttccgaccga aatatactca gccagaagtg gacgaaatat ttaaccttct
    44581 attaattcag atgtatcaac tgtcaaacga tcacgtaata aatagacgat taacattgct
    44641 gctaaagcac aaagtgactc agcaattaat aatgaccaaa tgacacctgt tagtccaaac
    44701 aaagcattca taataaataa tactggaatg ataattgcac cttgtaaaat ggccataatt
    44761 gtagcaccac gaccttgccc agtcgcttga agcataccag taaacaagaa acctatacca
    44821 tttaataata atgatgccat tgttactttc aaaataaatg tcgccatctc aacaatggct
    44881 tgatcagtag taaatagtcc gaccatatga tgtccaattg taaatacagc actcatacat
    44941 acaacaaaga taacgccgat agacatgata actgctttga taacgtcttt catacggcct
    45001 ttatttgcca taaagttata tgcaattagt ggtacaacac cttcacataa tcccatgata
    45061 ataagttctg gaaattgcac aagtctaaat gagataccat aacttgcaat cgcgaagttt
    45121 ccataatgtg ctaaaaataa atttaaaact aatcctgtga atcccattaa gatactcatt
    45181 aaaaatgcag gaataccgat tttaaagatt tcagaaagca tttctttatt aggtttcgca
    45241 agtttaatat taactgacac aacgtcacta tttttcataa aataaatgat aaagaacaga
    45301 gcagcagcaa cattactgat tgcagtaccc aaagctgcac caacaacgtt taaatcaaaa
    45361 ccaaaaatta aaattggatc taaaataata tttaagccta cactagctaa cataccaatc
    45421 atagaaacca ttggtgcccc aattgcacgt gcaaattgtt ctaatatgaa gaacaaaatt
    45481 acaaaaggtg cacttaaaaa cattactttc aaataattac ttgttaaagc taacgtttca
    45541 cctctcgccc ctaaaattgc tgcgatttga tcactgaatg gtaaagtaac taaaatcacg
    45601 ataagtccta gtgcaatacc accataaata gagaaactac ttacaaattt actcttacta
    45661 tagtctttcg cacctaataa acgtgaaata taagttcctg caccaacgcc aaataaatta
    45721 cctaacccca ttaagatagc aaatactggc agtgttagag agatagcaga aatcatgtgg
    45781 ctatcttcta aaaatcctat aaagtaaata tttaatatgc cataaataac gcttaataaa
    45841 gtccctatca tcattggcaa tgagaaatgc atcatcgctt taaatactgg cgatttctca
    45901 aaataatata attgttcgtc tttcattgtt caatactcct tgtcttttcc aataattagc
    45961 ttactaacaa ttagatatct aactataata ttaaagacaa agtgactgat ttctaccagt
    46021 cacacttatc atttattgta aactagataa cattttagtt aagtttgctt tcatttgttc
    46081 attttcttct tcagataact gcgatacgag tgtttgttcc atttcatcaa atatcgaagt
    46141 gaatgcttct acgagtttaa tcccagaggt agtcagccct atattctttc ttctcgtatc
    46201 ttgtgcatcg acatagcgat agatcagctt tttacgttca aggttcctta ataaattact
    46261 gacagttgga cctgttcgtt gtaatgcttt agcaatatca ttttgtgtca gtccatcttg
    46321 ttgatgtgca taaagataac ctaacgtatg accttgctca tttgtaatat caaattgctc
    46381 taacttttga tcagcctttt gtttcatctc atgactaatc attctaaata aatacgaata
    46441 agtgaattcc atttattgca ccaccatttc gttagatgtc taactaaata tactgcgaca
    46501 tttttaaatt gacaacatga atatctaatt tattttaaaa gttcctataa aatcttgcgc
    46561 cataaacatt ttgatatgca atttttcgga ttaaaatatg aaagagattg catatctacg
    46621 tttttaagaa atcgcttaca attacaaata ggaggatatt acgttgttaa gttatagaca
    46681 aatcgaaatt ttgtatcttt taattcatga gcaaacgttt attccaatca ataccattgc
    46741 taatcaactt ggtgtatcac ctcgtaccat tcaatatgat atagcatata ttgagcaata
    46801 tgctgaaact tatcattacc aagtcagccg taataaagct gcaggcatca aagtaacaac
    46861 agctcacgcg acactattaa atgaattaga gcacaatcta acaaatcaaa ttcatttttc
    46921 aaaagatgaa cgactcacac atatcgcttt aaagttattc gaaacaaccg atcctgtttc
    46981 aacaaagcaa cttgcgcaag atgttaatgt ttcgcgtcgg acaattgcag atgatattaa
    47041 aatgattcaa gcccaactag atcaatacca tttaaagtta aattatgtcc acaataaagg
    47101 attcaatatt attggtgagg aagatcatta tcgtaaagcg tatgcacact ttatacatca
    47161 atatatgaaa caagctgcac cttttataga ggcggatatc tttaattcag aatcaatcgc
    47221 attggttcgc cgtgccatta ttaagacatt aaatagtgaa aattatcatt tagttcagtc
    47281 ggctatcgat ggcttaatct atcatatact cattgccatt cagcgtttaa atgaaaattt
    47341 ttcgttcgat atacctatca atgaaattga taaatggcga catactaatc agtatgctat
    47401 tgcttcaaaa atgatagaaa acttagaacg cagttgtaat gtcacatttc cagagtctga
    47461 aattatcttt atcacgttac atttactagg ctctaaaatg actgaacata cagcatcttc
    47521 aattaccttt gaataccatg atttatcgca aaatatacat gaattgatca cttgtgttag
    47581 ccaagaatta ggcattgata tgtcaaaaga caacaagtta cataccagtc tgatcacaca
    47641 tatcaaacca gctatacatc gtattaaata cgatatgcta caacctaatc ctttgaggca
    47701 agaagttatg cgtcgctatc ctcaaatcat tgaagccgtt agcaagcata ttagtccaat
    47761 tgaacaagat gctgctattc gcttcaacga agatgaatta acatacatta caattcactt
    47821 cgcatcaagt atagagcgtg ttgcaacaca taaacaatca atgattaagg ttgtcttact
    47881 atgtggttct ggtataggca cgtcacaact tttaaaatca aaactaaatc acctgtatcc
    47941 tgagtttcac atttgggatg cctattccat ttatcaattg gaagaaagtc gattattgca
    48001 agataacatt gattatgtca tttcaacagt accttgtgaa atatcagctg taccagttat
    48061 tcatgtcgat ccatttatca atcaacaatc tcgtcaaaaa ttgaatcaaa ttatcaatga
    48121 ctcaagagaa caacgagtca tgaaaatggc aactgatggc aagtcactcg cagatttatt
    48181 gcctgaacat cgcatcatta taaataaaca accattatca attgaatccg caattgcagt
    48241 ggctgtgcaa cctttaatca atgatggcat tgtctattca aattatacag ctgcaatttt
    48301 aaaacaattt gaacaattcg ggtcatatat ggtcattagt ccacatattg cacttattca
    48361 cgctggtact gattatgtac agaatggtgt aggtttcgca ctaacatatt tcactgaagg
    48421 gattatcttt ggtagtaaag ctaacgatcc cgttcacctt gtaattacat tagcaacgga
    48481 ccaccccaat gcacatttaa aggcattggg acagttaagc gaatgcttaa gcaacgactt
    48541 atatcgacaa gatttcttag atgggaatat ttttaaaatt aaacaacaca ttgctttaac
    48601 tatgacaaag gaggcttaat aacgtgtcat tagacatttt gtcaacaaca cgcatcattg
    48661 taaaagaaca agtaaatgat tggactgaag ctataactat agcttctcag ccattactac
    48721 aagaacaaat tattgaacaa ggctatgttc aagcaatgat tgatagcgtt aatgaacttg
    48781 gaccttatat cgttatcgca cctgaaattg caattgcaca tgcaagaccg aacaatgacg
    48841 tacatcaagt tggtttaagt ctattaaagt tgaatcaaca tgtggcattt tgtgatgaag
    48901 atcactacgc atctctcatt tttgtattga gtgccatcga caatcattca cacttatctg
    48961 tattacaaaa tttagcaacc gtactgggcg ataaccaaac agtccagcaa ctattaactg
    49021 caacaaatgc acaagacatt aaaaacattt taaaggagca tgattaatat gaaaatttta
    49081 gtagtatgtg gccacggttt aggaagtagt tttatggtag aaatgaacgc acaagaagca
    49141 cttaggcaac ttaatgcacc atctgatatc gaagttgaac atagtgacat tatgacagca
    49201 agtccagaga tggctgactt gtttatttgt ggtagagatt tagctgaaaa tgccgaacgt
    49261 ctaggggatg tcttagttct tgataatatt ttagataaag ctgaattaca acaaaagctc
    49321 tcagaaaaat tacaacaact taacatgatt taaaggaggt acgacctatg caagcaatcc
    49381 ttaattttat agtcgatatt ttaagtcaac cagccattct tgttgcactg attgccttta
    49441 taggtttaat cgttcagaaa aaacctgccg caacgatcac ttcaggaacc attaaaacga
    49501 tattaggctt cttaatttta agtgcaggtg ctgatgtcgt cgttcgatct cttgaaccat
    49561 tcggcaaaat attccaacac gcatttggtg tgcaaggtat cgtacctaac aacgaagcta
    49621 tcgtctcact agccttaaaa gattttggaa caacagctgc actcatcatg gtctgtggca
    49681 tgattgttaa tattttaatt gcccgcttca ctaatttaaa atatatcttt ttaacaggtc
    49741 atcatacatt ttacatggct gcgtttttag caatcatttt aacagtcagt catattaaag
    49801 gctggctaac gattgttatc ggcgcactcg tattaggatt aatcatggca gtattacctg
    49861 cattactcca acctacgatg cgaaaaatta cagggaatga ccaagtagct ttaggtcatt
    49921 ttggctcaat cagttacttt gccgcaggtg ctgtaggtca attattcaaa ggtaagtcta
    49981 aatcaacgga agagattaaa tttccaaaag gcttaagttt cttacgagaa agtacaatta
    50041 gtatctcgat tacgatggca ttactttact tcatcgcatg cttatttgcg ggcgttagtt
    50101 atgtacacga atctattagt gatggtcaaa actttattgt cttttcatta attcaaggtg
    50161 tgacatttgc tgctggtgta tttattattt taacgggcgt tcgtttaatc ttagctgaaa
    50221 tcgtcccagc atttaaagga atttctgaaa agcttgtacc aaattctaaa cctgcattag
    50281 actgccctat tgtgttccct tatgcacaaa atgcagtatt aattggattc tttgtcagct
    50341 ttattacagg tgtcatcggt atgtttatct tattcttatt tggtggcgtc gtcattttac
    50401 ctggcgtagt tgcacacttc ttcttaggtg caacggctgc tgtattcggt aatgcaagag
    50461 gcggtattaa aggtgctatt gctggcgccg ctctaaatgg tatcctaatc acgtttttac
    50521 cattattatt cttgccattt ttaggcgaat taggtggtgc tgcaacaaca ttctcagata
    50581 cagacttttt agctgtcggt atcgtgttcg gtaacgcagt aaaatatatg ggattatttg
    50641 gtgcgattct atttattatt atcgtaggtg cgacaacaat tttattaaaa ggccgtcaaa
    50701 aagaacagca atagtgttaa cgtagaaata taaaacaccg tcacatattg agtgaatgcc
    50761 ccttttatca agaggaaagc cacttactta tggacggtgt tttgtattat attaaatgat
    50821 acttagccat actatcgaca gctgctaaaa ttgcttcttc ttgtgtcgca atcggttccc
    50881 aaccaagtaa tgtttttgca cgttcgttac ttacattgcg gttcatatct aataaaagtt
    50941 taccttcttt agcttgatga ttaaatttag cacctagact caaaataaag tctggtaatt
    51001 ttttagtaga aactttttga gctatttcag gtctcttttc tttaattaat tttgcaattt
    51061 ccaacaaatt aatttgtcca tcagccgtcg caataaatcg cttgccatta gcttgttcat
    51121 ttgtcattgc caaaatgtgc agttcagcta cgtctctcac atcaacaaca tttaacggaa
    51181 tttgcggtac acgtttcatt gaaccattca ataaattttc taataaatga aagcttcctg
    51241 aaacgtgtgc atctaatgat ggcccaaaaa ttgcaactgg attgattgtg gcaaattcta
    51301 ctgttgtatt ttcattctca acaaaatccc acgctgcctt ttcagctaac aattttgatt
    51361 tttcatatac tgataagcct ggttcatctt catttgtcca atgactttca tttgtgattg
    51421 aatttttatc tttattacta aaaccaactg caccaaagtt tgcagtcatt accacacgtt
    51481 taacacccgc atgttctgca gctcttaaaa tacgttgtat accttcaatt gcaggcttcg
    51541 ccatcacttc tgcatcgtct gttttaccga aaaacaccgg agatgctaca ctcaagacat
    51601 acttgcaatc tttcattgct tcatcccaat gttcatcttg tgataaatcc gcttcgacaa
    51661 acattaatcg ctctgtggaa atgccattgt cttgcattgt tttaattact ttatcagctt
    51721 tacttaaatc acgtatcgtc gtttgtacgt cataaccttg ttctaataat cgtgaaataa
    51781 ttcgcatgcc aacaaaccct gtaccaccgg ttgctaatac tttattattc atcacataat
    51841 ctcctctttg tgtctattga ttactcatta tagcagtcat tatattttgt atatatcaac
    51901 agacttatat tgcttaatat tctatatgaa ttataaaagt aaacatcatt atattcctta
    51961 aaatattaaa ctcatctatt ttcaaatacc caatcattta gaaacaaaaa ccatatatat
    52021 atcatacttt gaatagttaa aggcaataat ttccactcat atgttcaacc ttcaccaaac
    52081 actattgtca ccttttcaag agcaagcaaa cacttccaat gattcagcat acataaaata
    52141 acaatattaa cttaaaatca tatccactaa tgcttctacc gtaacattgc taaaataata
    52201 aacgatgtct aattgcttta actgatgcgt taaatcttct cttgtcattt ttgttccttg
    52261 taatgcttct tccacatctt ttatatcacc ttgtccaaag aaatccccat aaatacgaca
    52321 atctgcgata cgattttgtt caacagaaat tgttatgtct accgtacctg aagataatct
    52381 ttcacttcga ttgtattcat atttgggtga cttgccataa ttccaatccc aatttttata
    52441 ctttttatca gctaattcat caatcgcttc ccaatctgca tccgttaatt catatcgttt
    52501 cgcctcttta atgtcatcga ttcccaaaat ctgcgtcacc attaaatttt taaattcttc
    52561 aatggttata tcacgatact tttcatccag tgcttctcta agatgaccaa cacgtgcgcg
    52621 cacagattta atccctttcg atgcaatctt tttgcgatta ggctttaaca ctttatccat
    52681 tgcttcataa ttaacatcaa gtaatagcga ataaccgcca taaatacgat tattcattaa
    52741 tgtcattgcg gcgcctgaca cttttttacc attcaatgtt aaatcatttc taccgctttg
    52801 taccacatct gttgcaccta atgtatgcaa cgcctttata gctggttgat aaaatcgttg
    52861 aaaatcacca taaattgaag tgtcttgttc taaaatacaa cacatattaa cggcaccttt
    52921 atcaacatac acagcgccac caccagtatc tcggcgaact acttgaatac tgtgtttatc
    52981 tatataatct tgattcactt ctatagcagt attttgaaaa cgtccaattt ccacttttgg
    53041 atcacaataa taagggaata aaatatcttc atctaaaaag atatgctggt taacataaac
    53101 ttgcatagcg agtgcaatcg caccatcagt aatatattct ccatttctaa tcggttctat
    53161 taagtacatc ttctgtctct ccaatgttgt tatttttcgt tgtgctgtcg ttccggagtg
    53221 ctgctgtaat caatgttgag atatcctcag ttaaatgtat ggtacgttct tgaattgaat
    53281 tcggaatgcg atatgccttt ttattcatcg tcatataaag ggcattttca tttttacgtg
    53341 tcatacgctg aaaaggatgc ttcacaaatt gtggtgtagt ataaccaatt ccaatttcca
    53401 aatacaacac tttatcatct tgatgttgct ctagaaaagc attataacga tgtagttgcg
    53461 catgaaattc agcatcttca accatcccaa cttccgcttt acgtttattc acttccattg
    53521 gggcatcaca ttttggacat cttggaatca tctcccaagg tataagcata tcttgttgcg
    53581 caacaaccat tttacgaatt aaatcatcat tgcgatacgt ttgagcatga caatgctgac
    53641 tacactgttg cagtatatac tccccttgta tatgaaatac atgagtcata tcataatcag
    53701 ctacatcgaa agcattatct gcattcgtag ttataatgtg gtactgttta ccttccacca
    53761 aggattttaa agcgaggtaa gactgaccta caggttgatc taaatagttt aatgtaataa
    53821 aacgactctc aaatgcccaa tactcttgcc aactgccata aggatgtaaa ctcgcttgca
    53881 acatatcaaa gaagcgatat ttttcaataa aatctgggaa attttccgta aaacgctctc
    53941 ctacatatgt aaatccgtca gatgcagaca tgcctgcacc aattccaatc actatcgcat
    54001 ctgcttcatc aatcgcagta cgcaatactt cagcctgctt tgtcttgtca tccattaaca
    54061 gagacattgc attccactta ctactctgca tcacggttaa atgcctcctt atacagttgt
    54121 aaatccttat ctgtaaatac attgaacacg actttcaatg ttgaatttgt ttctttgaga
    54181 tagctttcta ctgttcgaac agcaatttct gctgcttcat cttgaggaaa agcaaataca
    54241 cctgtagata tacagcaaaa agcgacatga tttaaactat gttgatcagc caatttaaga
    54301 cagctaagat aacatttagc taacaagtcc tgattcatct ttgaaacagg caatcgacgt
    54361 atttgcggac caaccgtatg aattatatac tttgctgaca aattatatcc acgtgttatt
    54421 ttggctttac ctacaccttc attgcgccct tgttgtcgaa tgatctctgc acaatcaagt
    54481 cgaacttgaa cacccgcttt tgtatgaata atattatcaa tgcagtcatg attagcttgc
    54541 atacatccta gaaaacgact atttgcagca ttaacaatag catcgatttt taacgtcgtg
    54601 atatcacctt gccaaacaaa gatattgtca ccttttatcg gcttcaaatc cttcaaatcc
    54661 gtaacatgtt gacgattcaa atcacttaac atagcatctt gtacagctaa atattcatca
    54721 cttacaggta atgccggtct gacatttgct aagcctcgat ataattccca caaatcttca
    54781 aatgaagatg gcattaccaa tacgtcattg tcatttctct ctcgatgcat atcattgatt
    54841 aaatattcaa gtctcgcttt atttgatttt aacgtttcca tttaagcctc cggtaatgct
    54901 aggaatgctt ctttatcgac atcatccaat ttgaacaacc aattttcttc tggtttttca
    54961 gagtttaaaa ttgtcggttc ttcttccgct tttgtatttc gctcaataat cgttcctgac
    55021 aatggcgttt gcacatcaat gaccgttttc gatgcttcga tactcacaat ttcatcatcc
    55081 actttaactt catctggact tacgaattca acataaccta ctgtcccaat atcatcttgc
    55141 aattcaggtg tcatactaaa cacatacaaa tctcctactt tttctaccca taaataattg
    55201 gctaactttt tcatcttatc ttcatccttt ctcttctatt taaaatgcta caaataaatt
    55261 tccgcgagta attttaatgt tttacaacgc tgttcaatac ctggaataag cggtgctact
    55321 aacacctcat caacttcaaa cgtagcaatg aaatcatcta attgtgcttt aaccttttct
    55381 tgtgtacctg caatgatgcg tgcttgatgt gcttgaatca tctctttgtc tcgatcatta
    55441 agcttatact tttgtgctgt gtctactgaa ggaaaatctt caaattctgc aaattgtaat
    55501 ttacctaata accaaacatc taaggcatgt tgtaatgctg ctacttccgc ttcgttatca
    55561 gctacaatga caaatacaga tgccatcacc tttgcgtcca ttttaatcgt tgatgcttgg
    55621 aaatgttttt tgtaaatatc aatgttatcc ttcgctgcat gtatcgcatt tatatctggt
    55681 agcaaaaatg ttccaacaga aagccctata cctagttcgg cagctatttt ggcagatgtt
    55741 gcgctactac ttaataacca catttctgga aaatgatcaa tgtgtggttg gacacctaac
    55801 gtatgcgcac ttggtttatc ctttattgta agataatcac gtaataacga aatcgattca
    55861 tcgtaactat catatgtagg atttattcca tctaaagctt gctttaccat agtagtacct
    55921 ggattattac caatacctaa atcaatacga tttggatata acgctgccat cattctaaaa
    55981 tgctcagcaa ttttataagg tcgatagtgc ggcagcatca caccaccaga gccaactcgt
    56041 atgtgatttg tctgcgccaa tgtatgcatc atcaaaagtt ctggactact acacgcaaac
    56101 gctggtacat tatgatgttc cgtaaaccaa attcgcttaa agccaagtcg atctgctaat
    56161 tttgcaagtg tcactgaatc ttgcaatgcc ttttgtgcat ccttaccttc atctattaag
    56221 gcatagtcta atacgcttaa tttaaccaat ccgtcatctc caaacttatc ctgtcatgtc
    56281 aaaccgacat aacattttag cgttcttaat accattccct cttcatatac ccacgtatat
    56341 gataacgttt ttcaattaac ttttatatct tttcgccttt atttttcttt ttcatattca
    56401 tattttcaga cctacaaatg ccattcatta gtttaatttt aaaaatactt tccatgtacc
    56461 aattctattc tatcaagact gttcatcatt gattattcat tctttccatg gtactttgaa
    56521 aacaatattg aatagaaaag gaatcatagt atgtaccgat acaaaccact tttacaatca
    56581 atccacttac ctaatggcat caaaatttcg aatcgctttg tattatcacc aatgaccgta
    56641 aacacatcaa caaaagaagg ctatattaca aaagaagact tggcttatgc tgcacgtcgc
    56701 tctaattcag ctggaatgca agtcaccggc gctgcttaca ttgaacctta tggtcaattg
    56761 tttgaatatg gctttaatat tgatcatgat gcttgtatcc ctggattaac taacatggca
    56821 tctacgatga aacaacatgg tagcctcgct attatccagc tagcgcatgc tggtcgtttt
    56881 tcaaatcaag ccattttaaa ttttggaaag gtgtatggac caagccctat gacattgcat
    56941 tcaccgattg aacacgatgt tatcgccatg tctcatgaga aaatcaatag catcattcag
    57001 cagtatcgcg atgctacatt acgcgcgatt aaagcaggtt ttgatggtgt ggaaatttcg
    57061 attgcacaac gtttgctgat tcaaacattt ttctcaactt tttcgaataa acgtacagac
    57121 cattacggtg ccgactcatt aaaaaatcgt gcaagattat gtttagaagt tatgcgtgcg
    57181 gtacaagaag tgattgataa agaagcacct gacaatttta ttttaggatt tcgagcaacg
    57241 ccagaagaaa ccagaggcag tgatttagga tataccattg acgagttcaa ccaattaatc
    57301 aattgggtca tggacgtttc aaacatccaa tacttagcaa tcgctagctg gggtcgacat
    57361 atttatcaaa atacgtctcg tacaccaggt gatcatttcg gtcgaccagt taaccaaatt
    57421 gtctatgaac atttagcagg tcgtatccct ttaattgcaa gtggcgggat taattctcca
    57481 gagtctgctt tagatgcttt acaacatgca gatatggtcg gcatgtcttc gccttttgtc
    57541 acagaacctg actttgtgca taaacttgct gaacagcgac cacatgatat caatcttgaa
    57601 ttttctatgg cagatttaga agatttagcc attccacatg ctgcttttaa agatattgtt
    57661 aaaatgatgg attatgggga aggtctaaag aagcataccc gtgacgcttt acgtcaattg
    57721 gaacagaatt atcatgactc atcgtcttaa gactattttt catggaaatt taagacagtg
    57781 ttataatgac atcaatatca aaaataagga gcctattaaa tcgtgactga aattaaagat
    57841 aaagtaatta ctaaagatgc atttgcatta ccttacacaa ttattaaagc aaaaaatcaa
    57901 ccaacaaaag gcgctattgt ctacatccac ggtggtggat taatgttcgg taaagctaac
    57961 gatttatccc cacagtatat cgatatcctt acggaacatt acgatttaat tcaactaagt
    58021 tatcgtttat taccagaagt aagtctcgat tgcattattg aggatgtcta cgcatcattc
    58081 gatgcgattc aatcacaata ttctaattgc cctattttta cctttggcag atcatcaggt
    58141 gcatatttaa gtctattaat tgcacgagac agagatattg atggtgttat tgatttttat
    58201 ggttatagtc gcatcaacac tgaacctttt aaaacaacta atagttatta cgcaaaaata
    58261 gctcaaagcg taaacgaaac aatgattgca caactcactt caccaacacc ggttgttcaa
    58321 gatcaaatcg cacaacgttt tttaatctac gtttatgcac gtggcacagg taaatggata
    58381 aatatgatta acattgctga ttatactaat tcaaaatata atattgcacc agatgaactt
    58441 aaaacacttc cacctgtttt cattgcgcat tgtaatggcg attatgatgt tcccgttgag
    58501 gaaagcgaac atatcatgaa tcatgttcca cattcaacat ttgaacgcgt gaataaaaac
    58561 gagcatgatt ttgatcgtag acctaatgac gaagcaatca ctatttatcg taaagttgtt
    58621 gatttcttaa acgctataac aatgatgtaa tatatcatta ttttaaaaaa ctcaatttat
    58681 catcatgata aacagttatt cgatcaacca tattattcat acaatttatt aagctcataa
    58741 acagatacgc ctgtcccttt agttttcgct aatgagacag gcgctttatt tcactttact
    58801 atttttaaat tcaacactgt gatgctactt atatctgatg tgccgtgata acagcacgat
    58861 ttacatagca tattaaattc agaagatgaa tttaacttgc tttcaattgt gttcctttac
    58921 tttcagtcgc ttcaacacgc aacaagtcat ttataatacc tgtatagaag ttggcaagtt
    58981 ctgcaccttt acgtttgaaa tccaggaagt ccacaccgat aaaatcgaca tgatcccatc
    59041 cttgtatgat tggtttaact tgccagatac ctctgcgtgt ggcaggttca tcattcgtaa
    59101 cattaacaaa tggttgattg gacggatgta atgacgaaat cactggtacg acaccatcat
    59161 ttttacgcca ttcttctctt gcatcatgac caataattct actcgttgta gccattaaga
    59221 aaaatgtacc taaatcagga ttttcataac ctaatggacc agtatgagat gatacacctg
    59281 tataagtcgt atacgtaata ttaggattca tacttgtcat gttgttcaat tttgcagagc
    59341 catctaacgt taaatcatag gcagcattgt cgtctgatgt ccaaattttg cttttactaa
    59401 cgcgttttat atagtcaatg taactctcat ttggtaattg tttaaagccc cattgcgtta
    59461 atcctaaatc gatattcgaa tacttgttac ccataaatcg atttaaagcg aacatgattt
    59521 ttctaacagc ttctgtattt ccaaacttat cagctgcttg tgaaccatta tgtggtgttg
    59581 ctaatgttgt gattgatgca accatattgt tatgaccacc agtgaataat ggtgatattt
    59641 ctccaccatg cgctttatga taggcaattt cttctttgtt accatttctt aaaaactctt
    59701 ccattaaacg aattgtttga ccacccatac tatgccctac aagatgtacc tttttaccag
    59761 gttcccaatt aggcatgatt cctttataag tcttaccata gcgctcatgt ccgtatttag
    59821 ctgcatgtgc tgcgccataa tctacgcgac cacctttaat gtaataataa agttctacag
    59881 cgcgatcata gttactacca aatgcactta cacttgcttg atgtacatta tagccttgct
    59941 ttctcaattc ttcgataact ttaaatttat ttccacccca ataatttgga tataaagcag
    60001 gtgcattatc gcctactaat cctaaaaatc catgtacaaa aacaactgga tatttgttaa
    60061 gtggttgtac ttgattcgct ttaagtggtc gaaccttttt agcatctgct gctgcatttg
    60121 tagtcgaacg tgttttaggc gcttgaagcg catctttgat tagttgttta ttatcagtat
    60181 ttttatctgt cggtttctct ggggaatttt gaccatcgat atgtttactt aaatcgccaa
    60241 cttgtggttt ctgttcactt tggcgaacag tatcatcttg ctttgcatct atgatttgat
    60301 gcgcaggttg atgtgtatct tgttgatttg gatgtttgtc cgtggttgca tcttgtgctt
    60361 ttgttgattt aggtgcagtt ttatcattag acgggggtgt agttgaagtt ggtgctgctt
    60421 tatcatgatt gggtattacc ttttgtgccg gaatggatgg ttgtgattta tcaacatcat
    60481 tcgattgtga cgttgtcgat tgtgatccat tacgttcagc atgatgggta ttttgaacct
    60541 gatcattttg acgttcagta ttattagtcg cattattttg gtgtgactgt ttagaacctt
    60601 gtcgttcaga atcttgcttt acttgattat cattttgcat tctgattgtt ttactatttt
    60661 gtgttgattg atgcttcgat gattgaagcg tatctttacc ttctgtcact gttccacttt
    60721 taccattctc tttatgcata tcgtctaatt gctttcctga ctgcatttga tgtgacgtta
    60781 tcgcattccc ttgttctccc ggttgattta gtgtttcttt ttgtgccgct gcattagttg
    60841 atgttttttc cgaggcttgt gcttcatgtg atgacacaac aaacattgta gccgctaaca
    60901 ctgacaccac gcctattgaa tactttctaa tactatactt tctttcttct tgtcctctta
    60961 acatataatc acctcaaaag tatttttttg accccctctt gagaatgaaa aacaactgca
    61021 ccttcaatat aacatgacat atattatttt ttgattaaga ctacaaaatt aataaaaatt
    61081 taataatacg atattaaatc gatactaatc atttacaaat atgtgtattt ccaaataatt
    61141 tataaacact tttagttgtg gtgttgaatc aacacaacaa catttaagat aaatatttta
    61201 ttaagta
//
LOCUS       DALAGF010000004        49662 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.004, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000004 DALAGF010000000
VERSION     DALAGF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 49662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49662
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.004"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            138..1127
                     /gene="ccpA"
                     /locus_tag="O2692_000226"
     CDS             138..1127
                     /gene="ccpA"
                     /locus_tag="O2692_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCZ4297876.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(1225..2394)
                     /locus_tag="O2692_000227"
     CDS             complement(1225..2394)
                     /locus_tag="O2692_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HCZ4297877.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(2419..3051)
                     /locus_tag="O2692_000228"
     CDS             complement(2419..3051)
                     /locus_tag="O2692_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ4297878.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTHEDLKALTFDAHLTAFRD
                     AEDQYKALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGKLPYLIELGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFENYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            3220..4926
                     /gene="acsA"
                     /locus_tag="O2692_000229"
     CDS             3220..4926
                     /gene="acsA"
                     /locus_tag="O2692_000229"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCZ4297879.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRIFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            5366..7033
                     /locus_tag="O2692_000230"
     CDS             5366..7033
                     /locus_tag="O2692_000230"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCZ4297880.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVEGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKVREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWKKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            7328..10015
                     /gene="harA"
                     /locus_tag="O2692_000231"
     CDS             7328..10015
                     /gene="harA"
                     /locus_tag="O2692_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HCZ4297881.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKNYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSVASNQTNTNTSNQNTSTINNANNQPQATTN
                     MSQPAQPKSSTNADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDKDADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNN
                     HTGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLS
                     QSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            10146..10238
                     /locus_tag="O2692_000232"
     CDS             10146..10238
                     /locus_tag="O2692_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297882.1"
                     /translation="MKIILLLFLIFGFIVVVTLKSEHQLTLFSI"
     gene            complement(10218..11123)
                     /locus_tag="O2692_000233"
     CDS             complement(10218..11123)
                     /locus_tag="O2692_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCZ4297883.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            11505..12767
                     /locus_tag="O2692_000234"
     CDS             11505..12767
                     /locus_tag="O2692_000234"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCZ4297884.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(12861..14135)
                     /locus_tag="O2692_000235"
     CDS             complement(12861..14135)
                     /locus_tag="O2692_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCZ4297885.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            14307..14924
                     /locus_tag="O2692_000236"
     CDS             14307..14924
                     /locus_tag="O2692_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCZ4297886.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            15052..16518
                     /locus_tag="O2692_000237"
     CDS             15052..16518
                     /locus_tag="O2692_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HCZ4297887.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGVVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDK
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            16625..17755
                     /locus_tag="O2692_000238"
     CDS             16625..17755
                     /locus_tag="O2692_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ4297888.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(17716..17811)
                     /locus_tag="O2692_000239"
     CDS             complement(17716..17811)
                     /locus_tag="O2692_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297889.1"
                     /translation="MKPLVQSFTTSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(17905..19509)
                     /locus_tag="O2692_000240"
     CDS             complement(17905..19509)
                     /locus_tag="O2692_000240"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCZ4297890.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAV
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(19496..20656)
                     /locus_tag="O2692_000241"
     CDS             complement(19496..20656)
                     /locus_tag="O2692_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HCZ4297891.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKEHFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(20770..21216)
                     /locus_tag="O2692_000242"
     CDS             complement(20770..21216)
                     /locus_tag="O2692_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCZ4297892.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPQI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            21296..22039
                     /locus_tag="O2692_000243"
     CDS             21296..22039
                     /locus_tag="O2692_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCZ4297893.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVTRKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            complement(22255..22857)
                     /gene="rpsD"
                     /locus_tag="O2692_000244"
     CDS             complement(22255..22857)
                     /gene="rpsD"
                     /locus_tag="O2692_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCZ4297894.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(23101..23565)
                     /locus_tag="O2692_000245"
     CDS             complement(23101..23565)
                     /locus_tag="O2692_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCZ4297895.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQIVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            23702..25396
                     /gene="ezrA"
                     /locus_tag="O2692_000246"
     CDS             23702..25396
                     /gene="ezrA"
                     /locus_tag="O2692_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCZ4297896.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(25470..25610)
                     /locus_tag="O2692_000247"
     CDS             complement(25470..25610)
                     /locus_tag="O2692_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297897.1"
                     /translation="MIMLFSLLILIKTTHLITFIFKQLLFIFIVPFIYPYKKEARQRI
                     VG"
     gene            25759..26907
                     /locus_tag="O2692_000248"
     CDS             25759..26907
                     /locus_tag="O2692_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCZ4297898.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKTQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            26907..28130
                     /gene="thiI"
                     /locus_tag="O2692_000249"
     CDS             26907..28130
                     /gene="thiI"
                     /locus_tag="O2692_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCZ4297899.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAISAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            28174..28944
                     /locus_tag="O2692_000250"
     CDS             28174..28944
                     /locus_tag="O2692_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HCZ4297900.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAIIFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMAISMIAGSY
                     AGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            29042..29536
                     /gene="tpx"
                     /locus_tag="O2692_000251"
     CDS             29042..29536
                     /gene="tpx"
                     /locus_tag="O2692_000251"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCZ4297901.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSDASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            29659..30606
                     /locus_tag="O2692_000252"
     CDS             29659..30606
                     /locus_tag="O2692_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ4297902.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKIPSQFQGFMTELNQWMDTNRPKK"
     gene            30694..31896
                     /locus_tag="O2692_000253"
     CDS             30694..31896
                     /locus_tag="O2692_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCZ4297903.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(32142..32642)
                     /locus_tag="O2692_000254"
     CDS             complement(32142..32642)
                     /locus_tag="O2692_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCZ4297904.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            32783..33901
                     /gene="ald"
                     /locus_tag="O2692_000255"
     CDS             32783..33901
                     /gene="ald"
                     /locus_tag="O2692_000255"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCZ4297905.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            34025..34123
                     /locus_tag="O2692_000256"
     CDS             34025..34123
                     /locus_tag="O2692_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297906.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(34217..35272)
                     /locus_tag="O2692_000257"
     CDS             complement(34217..35272)
                     /locus_tag="O2692_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCZ4297907.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTECEVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            35447..36136
                     /locus_tag="O2692_000258"
     CDS             35447..36136
                     /locus_tag="O2692_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCZ4297908.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(36441..36854)
                     /locus_tag="O2692_000259"
     CDS             complement(36441..36854)
                     /locus_tag="O2692_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCZ4297909.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            37059..38357
                     /locus_tag="O2692_000260"
     CDS             37059..38357
                     /locus_tag="O2692_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCZ4297910.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            38620..39561
                     /gene="pde2"
                     /locus_tag="O2692_000261"
     CDS             38620..39561
                     /gene="pde2"
                     /locus_tag="O2692_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HCZ4297911.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGNEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVDSWDEFEQLATALRTKLN"
     gene            39582..42779
                     /locus_tag="O2692_000262"
     CDS             39582..42779
                     /locus_tag="O2692_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCZ4297912.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKKVGDQHRDIVQVFETHNDTYMDHLSISIQGRKHVWIQN
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTEKEINQLDINQEYLTQVDV
                     IAQKCDAELKYHQSLLPQYETPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNYKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNPSDIRLI"
     gene            43229..44458
                     /locus_tag="O2692_000263"
     CDS             43229..44458
                     /locus_tag="O2692_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCZ4297913.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTLAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            44653..45510
                     /locus_tag="O2692_000264"
     CDS             44653..45510
                     /locus_tag="O2692_000264"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCZ4297914.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            45510..46454
                     /locus_tag="O2692_000265"
     CDS             45510..46454
                     /locus_tag="O2692_000265"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HCZ4297915.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            46723..47691
                     /gene="pfkA"
                     /locus_tag="O2692_000266"
     CDS             46723..47691
                     /gene="pfkA"
                     /locus_tag="O2692_000266"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCZ4297916.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            47713..49470
                     /gene="pyk"
                     /locus_tag="O2692_000267"
     CDS             47713..49470
                     /gene="pyk"
                     /locus_tag="O2692_000267"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCZ4297917.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
BASE COUNT        17247 a         7140 c         9222 g        16053 t
ORIGIN      
        1 cgtttgtttt tatttcaagc ttttcgctaa atttttccaa acaaaaatat gttactgtaa
       61 attaaaatat ggtaaactat gaaaatgaaa tgaaaacatg ttattataat gaataaaacg
      121 tttacaagga ggaaattatg acagttacta tatatgatgt agcaagagaa gcgcgtgtct
      181 ctatggccac agtgtcgcgt gttgttaatg ggaaccaaaa tgttaaagca gaaactaaaa
      241 ataaagttaa cgaagtcatt aagcgtttga attatcgtcc aaatgctgtt gctagaggtt
      301 tagctagtaa aaagacaaca acagtaggtg tgatcattcc agatatatct aatatctatt
      361 attcacaact tgctcgtgga cttgaagata ttgcaacaat gtataaatat cactcaatta
      421 tttcaaattc agataacgat cctgaaaagg aaaaagaaat ttttaataac ttattaagta
      481 aacaggttga tggtattatt ttccttggtg gtacaattac tgaagaaatg aaagaattga
      541 taaatcaatc atctgtacct gtagtagtat caggaacaaa tggtaaggat gcacatatag
      601 catcagttaa tattgatttt actgaagctg cgaaagaaat tacgggagaa ttaattgaaa
      661 aaggcgctaa atcatttgct ttagtaggtg gagaacattc taaaaaagct caagaagatg
      721 ttttagaagg tttaactgaa gtgttaaata aaaatggcct tcaattaggt gatacattga
      781 attgttctgg tgctgaaagt tataaagaag gcgtaaaagc ttttgccaaa atgaaaggca
      841 atttgccaga tgccatttta tgtatcagcg acgaagaagc aattggtatt atgcatagtg
      901 caatggatgc tggtattaaa gttccagagg aattacaaat tattagtttc aataatacac
      961 gattagttga gatggttaga ccacaacttt ctagtgttat tcaaccatta tatgatatcg
     1021 gtgcagtagg gatgcgctta ttaacaaaat atatgaacga tgaaaagata gaagaaccaa
     1081 atgtagtttt acctcacaga attgaatacc gaggaactac aaaataaatt cacaaaatta
     1141 ggcattcatc taacgaccca aattatatgg gtgttggaag aatgcctttt atttatcttt
     1201 taaaatcgtt gcagattagg ttacttattg acgagtagat tcgtaccaac tcgctatatg
     1261 taaagctaat tttttatttt tttcactaat ttcttttgtg cgggggacat aggtataatc
     1321 atttaaacga tcttcccatc ttttaggtaa taattcagat gaataatgtt tccatttatt
     1381 aatccattct aacggtaaat aaccactttg aattggttga tcaattaaac ttaagaatac
     1441 atgactccat gcacgtggta cgactctcca aatattgtag cctccgccac caaacataat
     1501 tacctttcca ttcgtataag aatcagctaa atattttaca aaatatggaa tttcatataa
     1561 tgaatgtaac gtacaattta gatgagttag tggatcacga taatgtatat cgacaccatt
     1621 tacgcttaga ataatatcag gtttaaaact ctttacgaca ggctcaactg ttaatttaaa
     1681 acactccaaa aatgatgcat cttctgtata cggttcaagt gggacattta cagtgtgtcc
     1741 atagccgata tcttcaccgc gctcagtata gtgaccagag cctgggaaaa gaaattttcc
     1801 ggtttcatgg atagaataag tagtaacatg gttatcggca tagaaactcc attgtgtacc
     1861 atctccatga tgtgcatcgg tatctatgat taaaacgcgt tgattgtatt ctttagctaa
     1921 gtattgtgcg gtaattgcaa tatcattgta tatacaaaaa ccacttgctc gaccaggttg
     1981 agcgtgatgc aaaccaccac ctaagtgaca accatttaat actttgcctg acataataag
     2041 atctgctaaa gttaaagcgc ctccaacaat tgtggcacta tggcggtgca tatgcttaaa
     2101 ttgaccattc tcttcatcat ttaatccata tttcttagcc tcatcttcac tgataatgcc
     2161 atgtgaagca tgcttaatag cttcgacgta atcatattta tgaattaaca ttaattcgtc
     2221 atctgttgca attctaggtt gtactatttg ttctggagac aataaatttg cattcaaaag
     2281 tagctctgtt gttaatttta aacgcatttg attgaaggga tgttggtcat gaaatcgata
     2341 ttgtaataac ttatctgaat aaacatatgc agtttttgat gaatgttgtt gcatataatc
     2401 cctccgatat tccaaaaatt aaaagaaaaa ccgattcata taacgaatat catcaaacgc
     2461 ttgttgctgt tctaatgtaa tgtttttgcc aattcttgcc attaaacaat tagctggatg
     2521 acttgttatt tctggatcat ctgtagcgaa tatttcaagt ccaccagttg ccattaaccg
     2581 ctgcattaat tttttatagt caaatacatc taactttgaa ttttttaaat cccaatgcca
     2641 gtaatattct gtagttataa cgatataatt ctcgaattct ggtgtagaaa ggctaagttg
     2701 tatcagcttt tctgcaagtt gtaattgcct aaaattgatg ctgacttcaa ttgcccccaa
     2761 ttcgattaaa tatggaagct tacctgtaga ccagcgctca atttcatcag gatagtggaa
     2821 agtgacataa cccacaatga gttgatcttg gcgagcaaca taaattctac cttctggtaa
     2881 tgttgtaatt tctaacaaag ctttatactg atcttcagca tctctaaatg cggttaaatg
     2941 cgcatcgaaa gtaagcgctt tcaaatcttc gtgtgttaaa ggaccttcaa taacaaattg
     3001 cttgtcatga atgtaataat cttcggattg atacgtcttt aaatgattca tattttcaac
     3061 tcctcaatcg acgtcgtgat tgtattaaat tcattatata gaaaatttac aataattaat
     3121 actagaaaaa ggataaaagt aaaaattttg aataattaga aatgttatgt ataatattga
     3181 gaaagaaagc gttttcacat aacaaagggg gagtttcaaa tgaaagtcga agtttataaa
     3241 ggagcgcaag gtaaacataa ccttaaagat tatgaagaaa catataatac ttttgattgg
     3301 aaagacgtag aacaagcatt ttcttggagt gaaactggaa aaatgaacat ggcatatgaa
     3361 tgcatagatc gccatgtaga tcaaggatta ggggataaaa tagcgttaaa ttacaaagat
     3421 gagcacagaa aagaatcgta tacttataaa gatatgcaac ggttatctaa taaagcagcg
     3481 aatgttttgt ctgaacatgc agaagttgac aaaggtgaca gagtatttat atttatgtcg
     3541 cgtacacctg aactatattt tgcgttgtta ggtgttttaa aaattggtgc aattgttggg
     3601 ccgttatttg aagcatttat ggaaaaggca gttgcggata gattagagaa cagtgaagct
     3661 aaagtgttaa ttactaataa ggcattgtta cctcgagtac ctgtagataa attaccaaac
     3721 ttgaaaaaaa ttgttgtcgt agatgaggat gtagaagaca attacataga cttcattagt
     3781 ttgatggaaa ctgctagcga tgaatttgac attgaatggt taaagtcgga tgatggtttg
     3841 attttacatt atacatcagg ttctactggg caacctaaag gtgtattgca tgttcaacaa
     3901 gcaatgttag tgcactatat ttctggaaaa tatgtattag atttacaaga agatgatgtt
     3961 tattggtgta cagcagatcc aggttgggtt acaggaacat cttatggtat ttttgcacca
     4021 tggttaaatg gcgctacaaa ttgtatagct ggtggtcgct tttcgccaga acagtggtat
     4081 agtatgattg aagattttaa agtgacgatt tggtatacgg caccaacagc tttaagaatg
     4141 ttaatgagtg ctggtgacga tattgttgag aaatatgact tgtcatcgtt acgttcgatt
     4201 ctatcagtag gtgagccttt aaatcctgaa gttataaaat gggcgaaaaa agtatacggt
     4261 ttaacggtgt tagatacttg gtggatgaca gaaacaggtg gacatatgat tgttaactat
     4321 ccaacgatgg acgtcaagct tggctcaatg ggcaaaccat tacctggtat tcaagctgca
     4381 attatcgatg atgcagggaa tgaattacca ccaaatcgaa tgggcaacct tgctataaaa
     4441 aaaggctggc catcaatgat gtatcgtatc tggaagaatc cagaaaaata taaatcatat
     4501 tttattggag actggtatgt atctggtgat tcggcatata aagatgaaga tggttacttc
     4561 tggttccaag gacgtgttga tgatgtaatt atgacagctg gtgaacgagt tggaccattt
     4621 gaggttgagt ctaaattggt tgaacacgaa gcagttgccg aagcaggaat tattggtaaa
     4681 cctgatccgg ttcgcggtga aataattaag gcgtttgttg cactgagaaa aggatatgaa
     4741 ccaacagacg aattaaaaga agaaattcgt atatttgtta aagaaggttt gtcggcacat
     4801 gcagcaccac gtgaaatcga atttaaagat aaattaccta aaacacggtc aggtaaaatt
     4861 atgagacgtg tattaaaagc ttgggaatta aatttagatg ctggggattt aagtacaatg
     4921 gaataatgac atgaatgtta ttgaagattt ttttcgaaga ataaagggtg acaacatatt
     4981 tcatgtcaat gtttaaataa tcgtttactt tacgataagc aatataaaga actgttaact
     5041 tgtgtcatat catttcgtag aaagcatttg aaaatgatga cataacaata atggcatatc
     5101 tttatattgc tttttatttt taatatgatc tttggaagat gattatttta aataatagaa
     5161 aaatatagtt atcaatagta tcaagcgcta aaagttgcat aatacaaaac tttaataagt
     5221 gaatttattg caaaaatgaa agcgctaacc cgatttagtc gacaagtttt taacagttcg
     5281 ttattatatg aatgtaagta aaaatttctt agctacaact tacatattat aaatgcataa
     5341 attaaacaaa aaggggcgaa aaaagttgac tcatttatca gatttagata ttgcgaatca
     5401 atcaacacta caaccaatta aggatattgc tgcatcagta ggtatttcag aggatgcatt
     5461 agaaccttat ggtcattaca aagctaaaat cgacattaat aaaattacgc caagagaaaa
     5521 caaagggaaa gttgttttag taactgcgat gagcccaaca ccagctggtg aaggtaaatc
     5581 aacggttaca gttggtttag ctgatgcatt ccatgagtta aataaaaacg ttatggttgc
     5641 attaagagag cctgctttag gaccaacatt tggtatcaaa ggtggtgcga ctggtggtgg
     5701 ttatgcgcaa gtcttaccta tggaagatat caacttacat ttcaacggag atttccatgc
     5761 gattacaact gcaaataatg cattgtctgc gtttatcgat aatcatattc accaaggtaa
     5821 cgaattagga atcgatcaaa gacgtattga gtggaaacgt gtattagata tgaatgatcg
     5881 tgcacttaga catgtaaacg ttgggttagg tggacctaca aatggtgtac cacgtgaaga
     5941 tggctttaat attacagtag cgtctgaaat tatggcgatt ttatgtttaa gtagaagtat
     6001 taaagactta aaagataaaa ttagtcgtat tactattggt tacactagag atcgcaagcc
     6061 agttacagtt gcagatttaa aagtggaagg tgcacttgca atgattttaa aagatgcaat
     6121 aaaaccaaac ttagtacaat caattgaagg gacacctgca ttagttcatg gtggaccatt
     6181 tgcgaatatc gcacacggtt gtaactcaat tttagcaact gaaacagcac gtgatttagc
     6241 tgatatcgtt gtaacggaag ctggatttgg ttcagactta ggcgctgaaa aattcatgga
     6301 cattaaagtg cgtgaagcag gatttgatcc ggcagctgtc gttgttgttg cgacaattcg
     6361 tgcgttaaaa atgcatggtg gtgtagcgaa agataattta aaagaagaaa atgtagaagc
     6421 agtaaaagca ggaattgtta atttagagcg tcatgttaat aatattaaaa aattcggtgt
     6481 agaaccggtt gttgcaatta atgcatttat acatgatacc gatgcagaag tagaatatgt
     6541 aaaatcttgg gctaaagaaa ataacgtacg aattgcctta actgaagttt ggaaaaaagg
     6601 tggtaaaggt ggcgttgact tagcaaatga agtattagaa gtcattgatc aacctaattc
     6661 atttaaacct ttatatgaat tagaattacc attagagcaa aagattgaaa agattgtgac
     6721 tgaaatctat ggcggttcaa aagtaacgtt tagcagtaaa gcgcaaaaac aattaaaaca
     6781 atttaaagaa aatggttggg ataattaccc agtatgtatg gcgaaaacac aatattcatt
     6841 ctcagatgat caaacgttgt taggtgcacc atcaggattt gaaattacaa ttcgtgaatt
     6901 agaagcgaaa acaggtgcag gatttatcgt agcgttgaca ggtgcaatca tgactatgcc
     6961 tggtttacct aaaaaaccag cagcattaaa catggatgtt actgatgatg gtcatgcaat
     7021 tgggttattc taataaatca tgtcaattgt ttaataaaga taagtaaata gtttaataga
     7081 ccggactgtt ggagatgcat tatttcagca gttcggtttt ttgctgtgct aaaaatagat
     7141 tcaatttggc gaatctaacg acaatgtttg aaggtggtta attaatgtat atgaagataa
     7201 aaagtgggct tgaagaatag gaaagcgatg caatgaatat tccatattaa aaaaaataat
     7261 aaaataggtt gcaatattta attgggatgc gctacaatta acactaataa taggagttgt
     7321 taacaacatg aacaaacatc acccaaaatt aaggtctttc tattctatta gaaaatcaac
     7381 tctaggcgtt gcatcggtca ttgtcagtac actattttta attacttctc aacatcaagc
     7441 acaagcagca gaaaatacaa atacttcaga taaaatctcg gaaaatcaaa ataataatgc
     7501 aactacaact cagccaccta aggatacaaa tcaaacacaa cctgctacgc aaccagcaaa
     7561 cactgcgaaa aactatcctg cagcggatga atcacttaaa gatgcaatta aagatcctgc
     7621 attagaaaat aaagaacatg atataggtcc aagagaacaa gtcaatttcc agttattaga
     7681 taaaaacaat gaaacgcagt actatcactt tttcagcatc aaagatccag cagatgtgta
     7741 ttacactaaa aagaaagcag aagttgaatt agacatcaat actgcttcaa catggaagaa
     7801 gtttgaagtc tatgaaaaca atcaaaaatt gccagtgaga cttgtatcat atagtcctgt
     7861 accagaagac catgcctata ttcgtttccc agtttcagat ggcacacaag aattgaaaat
     7921 tgtttcttcg actcaaattg atgatggaga agaaacaaat tatgattata ctaaattagt
     7981 atttgctaaa cctatttata acgatccttc acttgtaaaa tcagatacaa atgatgcagt
     8041 agtaacgaat gatcaatcaa gttcagtcgc aagtaatcaa acaaacacga atacatctaa
     8101 tcaaaataca tcaacgatca acaatgctaa taatcaaccg caggcaacga ccaatatgag
     8161 tcaacctgca caaccaaaat cgtcaacgaa tgcagatcaa gcgtcaagcc aaccagctca
     8221 tgaaacaaat tctaatggta atactaacga taaaacgaat gagtcaagta atcagtcgga
     8281 tgttaatcaa cagtatccac cagcagatga atcactacaa gatgcaatta aaaacccggc
     8341 tatcatcgat aaagaacata cagctgataa ttggcgacca attgattttc aaatgaaaaa
     8401 tgataaaggt gaaagacagt tctatcatta tgctagtact gttgaaccag caactgtcat
     8461 ttttacaaaa acaggaccaa taattgaatt aggtttaaag acagcttcaa catggaagaa
     8521 atttgaagtt tatgaaggtg acaaaaagtt accagtcgaa ttagtatcat atgattctga
     8581 taaagattat gcctatattc gtttcccagt atctaatggt acgagagaag ttaaaattgt
     8641 gtcatctatt gaatatggtg agaacatcca tgaagactat gattatacgc taatggtctt
     8701 tgcacagcct attactaata acccagacga ctatgtggat gaagaaacat acaatttaca
     8761 aaaattatta gctccgtatc acaaagctaa aacgttagaa agacaagttt atgaattaga
     8821 aaaattacaa gagaaattgc cagaaaaata taaggcggaa tataaaaaga aattagatca
     8881 aactagagta gagttagctg atcaagttaa atcagcagtg acggaatttg aaaatgttac
     8941 acctacaaat gatcaattaa cagatttaca agaagcgcat tttgttgttt ttgaaagtga
     9001 agaaaatagt gagtcagtta tggacggctt tgttgaacat ccattctata cagcaacttt
     9061 aaatggtcaa aaatatgtag tgatgaaaac aaaggatgac agttactgga aagatttaat
     9121 tgtagaaggt aaacgtgtca ctactgtttc taaagatcct aaaaataatt ctagaacgct
     9181 gattttccca tatatacctg acaaagcagt ttacaatgcg attgttaaag tcgttgtggc
     9241 aaacattggt tatgaaggtc aatatcatgt cagaattata aatcaggata tcaatacaaa
     9301 agatgatgat acatcacaaa ataacacgag tgaaccgcta aatgtacaaa caggacaaga
     9361 aggtaaggtt gctgatacag atgtagctga aaatagcagc actgcaacaa atcctaaaga
     9421 tgcgtctgat aaagcagatg tgatagaacc agagtctgac gtggttaaag atgctgataa
     9481 taatattgat aaagatgtgc aacatgatgt tgatcattta tccgatatgt cggataataa
     9541 tcacttcgat aaatatgatt taaaagaaat ggatactcaa attgccaaag atactgatag
     9601 aaatgtggat aaagatgccg ataatagcgt tggtatgtca tctaatgtcg atactgataa
     9661 agactctaat aaaaataaag acaaagtcat acagctgaat catattgccg ataaaaataa
     9721 tcatactgga aaagcagcaa agcttgacgt agtgaaacaa aattataata atacagacaa
     9781 agttactgac aaaaaaacaa ctgaacatct gccgagtgat attcataaaa ctgtagataa
     9841 aacagtgaaa acaaaagaaa aagccggcac accatcgaaa gaaaacaaac ttagtcaatc
     9901 taaaatgcta ccaaaaactg gagaaacaac ttcaagccaa tcatggtggg gcttatatgc
     9961 gttattaggt atgttagctt tattcattcc taaattcaga aaagaatcta aataattaac
    10021 taaatatagc atatgtatga ttaactttgt agacaatgtg aaagcaatta atttataaac
    10081 tattgattgg tttaatggct ttcctttaga gtaaataaaa agaacagcag tgagaaattt
    10141 tctaattgaa aataatctta ctgctgtttt taatatttgg attcattgtt gtggttactt
    10201 taaaaagtga gcatcaatta acgctttttt cgatttaaca aatgtgattt aatatcatat
    10261 tttaatgcgt cgttgtattc tttttcagtg atttgatctt cgattaacat acgctttaat
    10321 acataatgtt gtctttgaat actatatttc aaatctttat ccgattttaa cgttccatct
    10381 ttttcgtagg gtgtatagcc atatgggctt tgcaacaaac cgataaggta tgcagattgt
    10441 gcaattgata aatcttttgg tggaatacca aatagactat atgaagcgga tgcaattccg
    10501 gaaatattag cgccattata atctctaccg aagggaacta tatttaaata tgtatatata
    10561 atttcatctt ttgagagtag gtgttctaat ctaattgcta ggcgaagttc atttgctttt
    10621 ctactatatg ttttttcgtt ggtaagaact tgatttttaa caagttgttg tgtaattgtg
    10681 ctaccacctg aactttgatc agtattaaaa atatcttgta tcattgctct taaaatcgcc
    10741 tttggtaaga tgccatcatg tttataaaat aaagtgtctt cagatgacgt taatgcttta
    10801 atgacatttg gacttgatgt tttagggcct ataatgagtg agttttgaga atggttatac
    10861 tcatataata aatttttgtt attatgatct aataattcat cgccaggtat ttgtcgaact
    10921 ttttttatta aagcatcatc tgataatgag tcggacgttt tagttaaatg atgaaaatat
    10981 aaagacatcg caatcacagc gatagcaacg atggcaatga aaatcataaa tataaaaaca
    11041 atgacatgtt tgatagcttt atatatcttt tcaaaatgat atatacgatg attcaattga
    11101 tgattgttgt cttgattcgt catctttaac ctcctaaatt acgtatagta taccatattt
    11161 aagcaatttg acttttattt taacgttaat tataattagg gaaaatgcat aaagaatagg
    11221 ataaaaggac aagtaatatt atttgaaatc atcagagagc tagtggtcgc tgtgaactag
    11281 tgtttttaat aatatgaata cacctttata actaacatgt cctacgttta ttaatgaacg
    11341 tatcattaat agagtgaaca ttatcttatg attatgttta agtttggtgg taacgtgcaa
    11401 tcgcgccctt acatatttgt tgtaagggtt ttttttacgt tcaaaaaact ttaaagtatt
    11461 ttaacaagta ttcaataaat aattataaat aggaggaaat aataatgacg aatgtattaa
    11521 ttgaagattt aaaatggaga ggtcttattt atcaacaaac tgatgaacaa ggtattgaag
    11581 atttattaaa taaagaacaa gtgacgttat actgcggtgc cgatccaacg gcagatagtt
    11641 tacatattgg tcacttacta ccattcttaa cattaagacg ttttcaagaa catggacatc
    11701 gtcctatcgt tttaattggc ggtggtacag gtatgattgg tgatccatca ggtaaatcag
    11761 aagaacgtgt gctacaaaca gaagaacaag tagataaaaa tatcgaaggt attagtaagc
    11821 aaatgcacaa tatttttgaa tttggaacag accatggtgc agtgcttgtt aataatagag
    11881 actggttagg acaaatctca ttaattagtt ttttacgtga ctatggtaaa cacgtcggcg
    11941 ttaattacat gttaggtaaa gattcaatcc aaagtcgttt agaacatggt atttcatata
    12001 cagaattcac atacacgatt ttacaagcta ttgatttcgg tcatttgaat agagaattga
    12061 attgtaagat tcaagtaggt ggatcagatc aatggggtaa tatcacaagt ggtattgaat
    12121 taatgcgtcg tatgtatggt caaacagacg catacggttt aactattccg cttgtaacta
    12181 aatcagatgg taagaaattt ggtaagtctg agtcaggtgc tgtttggtta gatgctgaaa
    12241 aaacaagtcc ttatgaattt tatcaattct ggattaatca atcagacgaa gatgtaatta
    12301 aattcttaaa atactttact ttcttaggaa aagaagaaat tgatcgctta gaacaatcta
    12361 aaaatgaagc accgcattta cgtgaagctc aaaaaacatt agctgaagaa gtaactaaat
    12421 ttattcatgg tgaagatgca ttaaatgatg caatccgtat ttcacaagca ttatttagtg
    12481 gtgatttaaa atcattatca gcgaaagaat taaaagatgg atttaaagat gtgcctcaag
    12541 tgacattatc aaatgacaca acaaatatcg ttgaagtcct tattgaaaca ggcatttctc
    12601 cttctaaacg acaagcacgt gaagatgtta acaatggtgc gatttatatt aatggtgaga
    12661 gacaacaaga tgttaattat gctttagcac cagaagataa aattgatggc gaatttacga
    12721 ttattcgtcg cggtaagaaa aaatacttca tggttaacta tcaataaata taattgcata
    12781 gctaaataaa ttagagccta ctcatattca ttcctaagaa tgtaatgagt aggctcttaa
    12841 tgtacttttc tgtctgtaaa ttatctaaag aaatctctat cgccttggcc aggtgattga
    12901 cgttctgatc ggctttgacg ttttggttgt tctttttgtt gttttaattt cactttaact
    12961 tcttttgttt taccatcacg gataacggta acagtgactg attcaccagg ttttttattt
    13021 tcatataaat agcttcttaa atcaacatca tctttaattt tcttgccatc aatttctgta
    13081 ataatatcac cttttttaag atcaatatca ctatcagctt tggcgacata aataccgtct
    13141 tctctatcag tatgaagttg ctcgcgctct tcttcaggaa tatctttcaa attaattaaa
    13201 ccaataccaa tcgaagggcg gtcaatttta ccatgtttta caagttgttc aattgttact
    13261 ttaacttcat tacttggaat agcaaacccg ataccttcaa cttgtgtcgc agcaattttc
    13321 attgagttaa taccaactaa attaccatta atatctacta atgcgccacc tgagttacct
    13381 gggttaatag cagcatctgt ttgaagaacg ctaacttttg tattgccacc agttgtctca
    13441 gcgtcaatcg tacgttcgct tgctgaaatg ataccagatg ttacagagtt agcaaattgt
    13501 aatcctaatg ggttacccat tgcgaatacg ctatcgccag tttgtacttt tgaagagttg
    13561 gcaaattgaa tcgctttaat accttttgta ttttcaattt taagtacagc aatatcagtt
    13621 actgcatctt taccaactaa tttcgcttta acttgttttt tattatgtaa ttggactcta
    13681 atttcatttg cgccatcaat aacatgattg tttgtaacga tataagctga attgttgttt
    13741 atttgataga taacacctga acctactcca gcttcagatg gtttagatga tttgcctttt
    13801 aataagtcgt ctacacttga tgctttttgc atgttaataa ctccaacaat tgtaggggag
    13861 acagatttta tcatttcatg aacggtaccg aatttcttgc tttgaccgtc taattgattg
    13921 ccacctttat tatttgttgt ctgaacagtt gaaccatctt tatttaaaat tgtactattt
    13981 aatactttgc ctataccaag tactagaagt gcaccaataa ttccagcaat caatgcaacg
    14041 atgactgttt taaaccatgg aaatttaggt cttctgtatc taggtgtttg gctatggttt
    14101 gttgtagaat gatctgtatg attaaaatct gacatactta acctccatta tatgatttat
    14161 atatgcttta attatgtctt ttttttataa ttattgctag taatacacta tacaacttta
    14221 gatgtatttt cagactatct tgttctagta aaaatatagt gataaaatct agttgttata
    14281 aacaaataag ttaagtaggt ggcacaatgt attcagtgat tagtaagatt ttgaatttca
    14341 ttttagttaa aatgtcaaaa tcattatacg taatcggtaa agataatatt cctaaggata
    14401 gtaaatatgt cgtaacttgt acgcatgaaa gttataacga agtcattatg ctaggtatgg
    14461 cattatatcc taatcaaatt cattatatgg ctaaaaaaga attattcaaa aataaatgga
    14521 ttggtaaatt tttaacgtct ttaaatgcat ttcctgtaga tcgtgaaaat ccaggaccta
    14581 gtactttaaa acgcccaatc aatttgttga aagataataa gacagttggt atcttcccaa
    14641 caggttctcg cacatcgcaa gaaggtgcac ctttgaaacg tggtgcttca acaattgcaa
    14701 tgttaagtaa gtcaccaata ttacctgttg cttatgttgg tcctactaag attcacggtc
    14761 ttttaacagg acaagcatac ataaatattg gcaaaccaat cgatattaac gatttaccta
    14821 aagatttaaa acgaaacgag cgtatcgatt atattacaaa agagattgaa acacgaacag
    14881 caaagttaca acaagaattg catgaaattg taaaaagttt ataaaataac ttgaggtaga
    14941 aatatagctg gttagtaagc ctaggtgact ataacggagt atcgaagtag ttattctagt
    15001 tacattagta atctttatat ttctacatta ttttaagaag gatgacattc aatgtataaa
    15061 tttttccaaa atttagggcg ttcgttaatg ttaccagttg ccattttacc ggctgcagca
    15121 attattgccg gaattggtaa tacgttaaat gcacttcatg cgacgccgaa aatcgcaatg
    15181 ttctttacta cggtaggtac tacgatttta gagcaattag gtattttatt tgcaattggt
    15241 gtagctatag gaatggcaaa gaaaaatgat ggtgtagtcg cgcttgcagc tacgttagga
    15301 tattttttag ttacagttgt attatcacca atgaaattag cgcctttatt aggaatgaaa
    15361 gcttcggaaa taaattcagc ttttgaaaag atgaataatg gcaatgtctt tgttggtatt
    15421 gtaataggtt taatagcagc ttatgcatac aataagttta gtgaaacaga attaccatta
    15481 gcattatcat tttttagtgg taaacgtcta gttccaatta tgactgcatt ttactgtaca
    15541 tttttagttg tcatattgtt attcttatgg ccactacttt attcatggat tgtaaaattt
    15601 ggtgaatcta tagtcggttt aggatcgttt ggagctttca tatatggtgt tgccaataga
    15661 ttattaattc ctacaggatt acatcatgca ctgaatagtg tgttctggtt tgatacgatt
    15721 ggtattaatg atattggtaa attccaaagt ggcaaagatg ctataaaagg tattacagga
    15781 cgctatcaag cgggattctt cccaatcatg atgttcggta ttccagcggc tgcgttagca
    15841 atgtatcata cagctaaaac tactcagaaa aaacaagttt acggttggtt cctagcgagt
    15901 tcagttgcag catttttcgt tggtgttaca gagccgattg agtttgcatt tatgtttgtt
    15961 gcgccaatct tatatgttgt tcatgcgtta ttaacaggat tatctttatt tatcgcagct
    16021 acattccatt ggacagctgg attttcattt agtgcaggtt tgatagatta tgtgttatca
    16081 ctaattaatc cagtatcaaa tcatccattg atgttgcttg ttcaaggtgt tgtatttttc
    16141 atcttatact atgtgatttt ccgtgtagta atccaagtat ttaacttgaa tacgattggt
    16201 agaggtgaaa atgaattagt tgacccaaca gttgtaaaag ataatattgc tcctggtgaa
    16261 aatgatatta aacaaagtaa atatcatcaa catgctatac aaatattaga aggtttaggc
    16321 ggtcaagaga atattgttaa tttaaccaat tgtgcaacaa ggttgcgtct agagttaaaa
    16381 gacacatcta ttattgataa acagaagatt aaaaacgccg gtgcagttgg tgttactgtt
    16441 aatggcaaac attcaactca agttattgtc ggtacacacg tacagcaggt tgctgatgaa
    16501 attgaaaaac atttataaaa tttaaacagc taaagcgcaa atctaacttc aatagggttt
    16561 gcgctttttt tagtcaaata gtagaatgat ttatatctga attttcagaa atggagtggg
    16621 gattgtgaag aaaattttat ttgatgttga tggcgttttt ttaagtgaag aacgttgttt
    16681 cgatgtctct gcattaacgg tatatgaatt attaatggat aagtgttatt taggtcttca
    16741 ttcacatata gattgggaaa ctttgactga taacgatata caagacatta ggaatcgaat
    16801 ttttcaaaaa gataagattt taaacaaatt aaaatcgcta gggctgaatt caaattggga
    16861 tatgttattt atcgttttta gtattcattt aattgatatt ttgaaaaagt tatcacatga
    16921 tgaaattgag gcattcatgt atcaagatga gcctgtcgaa ttgaagttgc aaaatataag
    16981 cacaaactta gcagactgtt ttaatttaaa tgaacaacta cctttacaat ttttggataa
    17041 tgtaaaagtt ggtaaaaata atatttatgc tgctttggaa gagtttgcaa caacagagtt
    17101 acatgtttcg gatgctactt tatttagttt gaagggtgca ttatggacgt tagcgcaaga
    17161 agtttatcaa gaatggtatt taggatcgaa gttgtatgaa gatgttgaaa agaaaatagc
    17221 acgaactact tttaagacag gttatattta tcaagaaatt attttgagac cagtagatga
    17281 agttaaggta cttctgaatg atttaaaagg tgctggtttc gaattaggta ttgcaacagg
    17341 tcgtccttat actgagactg ttgtgccatt tgaaaattta ggattgttac catattttga
    17401 agctgatttt attgcaacag caagtgatgt tttagaagca gagaatatgt atccgcaagc
    17461 acgaccatta ggaaagccga atccttttag ttatatcgca gctttatatg gtaataatcg
    17521 cgataaatat gaatcttata tcaataagca agataacatt gtaaataaag atgacgtatt
    17581 tatagtaggc gattcgttag ctgacttatt aagtgctcaa aaaataggtg caacgtttat
    17641 tggaacatta acaggtttaa aaggtaagga tgctgcaggt gagttagaag cgcatcatgc
    17701 cgactatgtt attaatcatt taggtgaact tagaggtgta ctagataatt tgtaatttga
    17761 ttgttgtttg acagcataac ttgtagtgaa tgattgaacc aaaggtttca tattgagtta
    17821 caatgaaatt aataatgaaa aaatgccaag aagcaatgga agtaatccaa tgtcttcttg
    17881 gcattttgaa tttacataaa ttgtttatga ctgtaccgtc aattcagttg tgaaaatttg
    17941 attgtattca ccaacttgtt taagttcatc aattatattg tttgaaacag gttgatcaac
    18001 ggataaaatc attagcgcat ctccgcccgc ttcagttcta cctaaagtca tagatgcaat
    18061 gttgatattg tatttaccta acaatgcgcc agtttttcct accatacctg gagtatcatt
    18121 atgatatgac acaatttgat attgatttgg ctttaagtct acagaaaaat tattaattct
    18181 aacaattctt ggaccgaaac ctgtaaagac agaagcgcca actttaacgg aatcgctatc
    18241 gtttgatagt tctacctcta agtagttact aaaacctgtc tctgctttat tattttcaat
    18301 atttaatgtc acttgttgtt gatttaacaa cattagagca ttgataatat tgacttcagg
    18361 accaagatca tcttttaaaa tatgagtaat aattgtacgt gttaataaac tactatcaat
    18421 agaggctaag tcgccaccgt acgtaatttt aatagagctt ggtgcattgt acattaattg
    18481 aatagctaat tcaccaactg tttggcttaa attgatgaat gattttacag tatcatctat
    18541 attgcttaag tccattttag gtgcattcac tgcatgcgtt acagtaccat caattaaaat
    18601 ttcgatgatt tcatttgaaa cagaaattgc cactttttct tgagcttcga ctgttgaagc
    18661 acccaaatga ggtgtaacaa taattttatc atgtgcaaca agaggcgagt cagttgcagg
    18721 ttcatgttca aacacatcga tagctgcccg actaatttgt ccttcgtcta atgcttttat
    18781 tagcgccttt tcatcaataa taccaccacg tgccacattg attatttgca aactaggttt
    18841 tgctttggca aaaaagacag cattaattaa gccttttgtt ttaggtgtta gtggtgtatg
    18901 taatgtaacg aaatcagaat gttgggcaat ctcatcaact gttgccttcg taatgcttaa
    18961 agattttgct ttttcatccg ttaagtaagg gtcaaaagct agtattttca ttccgaaact
    19021 ttgcgcacgt ttagcaacac ctaaaccaat tctaccagca ccaatgacac ctaatgtttt
    19081 atgataaagc tcagtacctt taaatgcatt tcgattccat tctttatttg taagtgattg
    19141 gtgtgcttgc ggaatatttc gtgccattga taataacatt gccagtgtat gttcagtagc
    19201 tgaaatcgta ttaccatctg gggcattaat aactaaaata ccttttaatg ttgcagcatc
    19261 aatattaata ttatctacac caacaccggc gcgtgcgatt acttttaaag aatcagcagc
    19321 atttatgata ttttcagtaa ccgtagtttg actacgaacg attaaagcat ggtatgaagg
    19381 tataattttg attaatgctt cttcggacaa gccagtttga atatctacat tgaattgttc
    19441 gtgatctaat aatgctttga taccatcttt tgatatggga tctgcaacga gtacattaaa
    19501 ttgcttcatg aataacctcc atatattttg atataccttt accgatatag ttaactttac
    19561 ggtgttcagt taaaataatt tctaaagcag atactaccga taaaatatcg aaaggactaa
    19621 ttttccccat atgaccaatt cttaaaattt gacctttaag atggccttga ccaccagcaa
    19681 ttgttatttt aaagcgattt ttaagttcat ctttgattat tttcacttca tcatttgtat
    19741 taggtttgaa tgctgtaacc gttggagatg catctttatc attgactaat aaagttaaat
    19801 ctaatgcttt taaggcgctt cttaatgcat ttctaattgc atagtgtcgt gctattacgt
    19861 gattgaaacc ttctgctttt acggtttcaa cgtatgcatt tacacctcta aataaagaca
    19921 catttggtgt gaacggtgta gaattgtcag cttgtgacga aatgtattta tttaagtcta
    19981 gataaaattt tggcgtagtt acttctttga aatgttcttt tgcacggtgg ctataagcta
    20041 caaaagctaa tcctggaggt aacataattg ctttttgact accagaaaca agtacatcaa
    20101 ttttatcttt gttaatgtca acatcaacag caccaatgca acttacgccg tcaactacaa
    20161 aataaatatt actattaaat tgattaatgg catttcctaa ctcgtgaata gggtgtagca
    20221 ctgtcgtaga tgtttcgcaa tattgactaa atactgcttt aacttcaaca tttaaagttg
    20281 aaaggaaatt gatgaaatct ttgacatcta cagcttctcc ccatgttacg tcataaatat
    20341 gcacattttt gtaataagtt tgtgcaattt gtttaaatcg gttaccaaag gcacctgaaa
    20401 caatgacaac gaagtgatct tcagggttta caatgttcaa catactagcc tccaagacgc
    20461 ttgtaccgct agatgttaaa ataagtacat cattttgact cccaaatatt ggctttagac
    20521 cttgaaatgc ttgttgtgcg atgtcttcaa aatctttaga acgatgacca accataggtg
    20581 cttgaatttc tctcataatg gcatcaggta caggggttgg gccaggtgtt aataacaacg
    20641 gttgatgata atacatttat atgcctccta taaataagat ttcactattt tagcaaattt
    20701 tctgaaaatt taaaagccta aaaaagtgat aacgctatat tgtcgaacaa acgttatcac
    20761 ttaacgattt tatttggcct gaatgatggg atagattttt atgtccacat tatttcttac
    20821 agcatttgaa atcatgcaat tattatctgc aattgttatc aattttggta atcgcttttc
    20881 taattgtgct atttgatcac ttggaatttg aatttgagga tggtgcacaa tttttgacat
    20941 actgaatttt ccgttattta aacaagctgt tccaatcgat tgttgttcaa ttgaaatatc
    21001 tgtgaacttt gcacgttcaa gagtagctgc taatgagatg atataacatg atgaagcggc
    21061 tgatactaac atttcatcgg gatttgttcc tataccaaca ccacctaaag aagcaggtat
    21121 agaaatattc tctgaaagta tgtcgccttg aacgtttccg acattgttac gaccaccttg
    21181 ccaagaagtt tggactttaa agtcatgttg atgcaattgc tttaacctcc aatataattg
    21241 tgatagttta attttagaat agtttatcaa agtttaacag aaaggtgact tatcaatgac
    21301 tctgaataaa ctgaaagatg aattacaaat tgtttcgcac cgtggattgc cgagtgattt
    21361 tcctgaaaat acaatggtcg gttatcgaga ggtaatgggg ctcaatgttg ctatgttaga
    21421 aatagatgtt catttgacca aagaccaaca ttttgttgtg atacatgatg aaacaattga
    21481 tagaacatcg gatggtaagg ggcgtattgc tgattacaca ttatcgcaat taaaatcatt
    21541 tgattttggt agttataaag atgttgcttt taagggagaa cgaataccca cgttagatga
    21601 agtactttca ttatgcttaa agtatgataa aaagctattg attgaattga aaagtccgaa
    21661 tttatatcca gaaatagaat gtaaattgct ggcatttttg gaagaaaaga aagtcgatgc
    21721 gacacaagta gttatacaat cttttgatat cgaatgcatc gaaaagttaa acacattggg
    21781 cagtatatat gaattaggtg tgctttgtag taaacgtaat tattggtata aaaagccaaa
    21841 cttttcaaga attgctcaaa ttgctagtta tgttaatcct aattatgcat tagtgaccag
    21901 aaaatttgtt gataaagcac atcatcatca attacaggtt atgccataca cagttaataa
    21961 attgaaaact ggagaaaaat taagacaaat gggtgtagat ggattaatta cagataatcc
    22021 gaaattattt attaagtgag acgatgaatg gtaatttgat agagggatga ggattcgtat
    22081 atcgcaattg ataattttaa aaactaatga tgaaatacta aagataatta aacaaataag
    22141 acttgtaaat tctaagttga ttgtaaaata gggtagctcc atataatttt gaatctatca
    22201 tttttaaatt aataaaaaaa acacccacaa ttgtgggtgt ttgtattgaa cgtattatct
    22261 tgagtagtac tcaacgatta attgttcgtt aatttcagca ggtaattcgc tacgttctgg
    22321 taaacgtacg aaagtaccag ttaagctgtc agcatcaaag tttaagtact caggtacgaa
    22381 attgttgatt tcaactgatt caacgatgat gtttaatttt tgagattttt cacgaactga
    22441 aattgtttga ccaggtttaa cagaataaga tggaatatca acacgtttac catctactaa
    22501 gatatgaccg tggttaacta attgacgtgc ttgacgacga gtacgagcta aacctaatga
    22561 ataaacaaca gcgtctaaac gacttgctaa taagatcatg aagttttcac cgtgtacacc
    22621 gaatttttta ccagcgatgt caaatgtgtt acggaattgt ctttcagtca ttccatataa
    22681 gtaacgtaat ttttgttttt cacgtaattg taaaccatat tctgataatt ttttacgttg
    22741 gtttggacca tgttgtcctg gtgcgtaagg acgtttttct aattctttac cagtaccgct
    22801 taaagagata cctaaacgac gagatttttt ccagtttgaa cctctgaatc gagccataat
    22861 aagactcctc ctttaattct tttttgttgt tatgaataaa caaaaaagag tgttgcatgc
    22921 ttactaagat atattgtttt atgtgtcttc accttatagc gacagttacg tgacacgtcc
    22981 gcattgggaa caacatagcg ctcaataata tacaactgct actttcgtta attcacacaa
    23041 agattattgt aacattttcc tgctaactgt caacacttta ttgaaaatgc tgatgtcctt
    23101 ttatgcgagt tgcttttcaa taattttaac aattgcttca agatgttctt tgtcattgtc
    23161 atcaaatcga tccgttatag gggcatcgat atctaagacg ccgataattt tatcatcttt
    23221 gaaaattgga acgacaatct cagacttact attagcatca caagcgatat gtccttcgaa
    23281 ttgatgaaca tcagctacaa tttgtgtacg acgttctgaa acggctgtac cacatacacc
    23341 ttttccaatt ggaatgtgga cacaagcggg gtgtccttgg aaaggtccaa gtataagttc
    23401 attttgttcc aataagtaaa agccaaccca attaatttga tctagattat catttaataa
    23461 ggcagacatg ttacttaaaa tagcaatcat gtgatgttca tcttcaataa ggcttgctgc
    23521 ttgtttcttt aataatgtgt agtttgttgg gttaattgtt gtcatattta aaacctctta
    23581 ttttcataga ttttataata gtatagaatt tcgaaaatat tatggcaata aattcatttt
    23641 ataatttatc cttaagcaaa attacgttat aatagtaatg ataaattagg aggagaagca
    23701 tatggtgtta tatatcattt tggcaataat tgtgattata ttgattgctg taggtgtatt
    23761 attctattta cgttcaaata aacgacaaat tattgaaaaa gcaatcgaac gtaaaaatga
    23821 aattgagacg ttaccttttg atcaaaacct tgcacaatta tctaagttga atttaaaagg
    23881 tgaaacaaaa acgaaatacg atgcaatgaa aaaggacaac gtagaaagta caaataagta
    23941 tctagctcct gtggaagaaa aaatccataa tgctgaggct ttattagata aatttagttt
    24001 caacgcatct caaagtgaaa ttgatgatgc aaatgagttg atggatagtt acgaacaaag
    24061 ctatcagcaa caattagaag atgtaaatga aattattgcg ttatacaaag ataatgatga
    24121 attatatgac aaatgtaagg ttgattatcg tgaaatgaaa cgtgatgttt tagcaaatcg
    24181 tcatcaattt ggtgaggcag caagtctact tgaaactgaa attgaaaaat ttgagccaag
    24241 gttagagcaa tatgaagtac taaaagctga tggtaattat gtacaagcgc acaaccatat
    24301 agctgccttg aatgaacaaa tgaaacagct aagatcttat atggaagaaa taccagaatt
    24361 aattagagaa actcaaaaag aattacctgg tcaattccaa gatttaaaat atggttgccg
    24421 tgatcttaaa gttgaagggt atgatctgga tcacgtgaaa gtagacagta cattacaaag
    24481 cttaaaaaca gagcttagtt tcgttgaacc attaattagc cgcttagaat tagaagaagc
    24541 taatgataaa ctagctaata tcaatgataa gttagatgac atgtatgatt taattgaaca
    24601 tgaagttaaa gctaaaaatg atgtcgaaga aacaaaagat atcattacgg ataacttatt
    24661 caaagctaaa gacatgaatt atacattgca aacagaaatt gaatatgtac gtgaaaacta
    24721 ctatataaat gaatctgatg ctcagagtgt tcgtcaattt gaaaatgaaa ttcaaagttt
    24781 aatttctgta tatgatgata ttttaaaaga aatgtctaaa tctgctgtgc gatatagcga
    24841 ggttcaggat aatttacaat atttagaaga tcatgtcaca gttattaatg acaaacaaga
    24901 aaagctacaa aatcatctga ttcaattgcg tgaagatgaa gcagaagcag aagacaatct
    24961 gctacgagta caatcgaaga aagaagaagt gtatcgtcga ttacttgctt ctaacttaac
    25021 aagcgttcct gaaaggttta tcatcatgaa aaatgaaatt gatcatgaag ttcgtgatgt
    25081 taacgaacaa tttagtgaac gtccaataca cgttaaacag ttaaaagata aagtgtctaa
    25141 aattgtgatt caaatgaata catttgaaga tgaagcaaat gatgttcttg ttaatgctgt
    25201 ttatgcagag aaattaattc aatatggaaa tagatatcgt aaggactata gcaatgttga
    25261 taagagctta aatgaagctg aacgattatt taaaaataat cgctataagc gtgcgattga
    25321 aattgcagag caagctcttg aaagtgttga gccaggtgtt actaaacata ttgaagaaga
    25381 agttattaag caatagaaac tagtatgtag ttatacttaa ataatatgag cactctgtca
    25441 aattggactg atgagtttaa taattgaagt tagccaacga tacgttgtct agcttctttt
    25501 ttatatggat aaatgaaagg gacaataaat ataaatagca attgtttaaa gataaacgta
    25561 atcaaatgtg ttgttttaat taatataagt agtgaaaaaa gcataatcac acagctgttt
    25621 aaatagagtg aaatagtcta attcttattt aataagtaga aataagatta ttttgcgcta
    25681 taacctttat tttttgtcgg ataatagtat aatgcttgag taaaacaatt ttaacgattg
    25741 atgattaatg aaagagaagt gtttattttg atatatctag ataatgcggc aacgacgaaa
    25801 gcatttgaag aagtgttaga tacttattta aaagtaaatc aatcaatgta ttataatccg
    25861 aatagtccgc ataaagctgg tttgcaggca aatcaattac tacaacaagc aaaaacccaa
    25921 attaatgcaa tgattaattc aaaaacaaat tatgatgttg tattcactag tggtgcaact
    25981 gaatccaata atcttgcttt aaaaggtatt gcctatcgta aatttgatac agcgaaggaa
    26041 ataattacat ccgtgttaga gcatccgtcc gtattagagg ttgtaagata tttggaagca
    26101 cacgaaggat ttaaagttaa atatgttgat gtaaagaaag atggcagtat taacttagaa
    26161 cacttcaaag aattaatgtc agacaaagtc ggtttagtaa catgtatgta tgtaaataat
    26221 gtaactggac aaatacagcc tattccacaa atggctaaag ttataaaaaa ttatcctaag
    26281 gcacattttc atgtagatgc ggttcaagca ttcggcaaaa tttcaatgga tctcaataac
    26341 atagatagta ttagtttaag tggacacaag tttaatggtt taaaaggaca aggcgtctta
    26401 cttgtaaatc acattcaaaa tgttgaacca actgtccatg gtggtggtca agaatatggc
    26461 gttagaagtg gaacagttaa tttgccaaat gatattgcaa tggttaaagc gatgaagata
    26521 gctaatgaaa actttgaagc attgaatgca tttgttactg agttaaataa tgacgtccgt
    26581 caatttttaa ataaatatca tggagtttat attaattctt caacttcagg ttcaccattc
    26641 gttttaaata ttagttttcc tggcgtaaaa ggtgaagtat tagttaatgc tttttcaaaa
    26701 tatgacatta tgatatctac gacaagtgct tgttcatcta aacgtaataa attaaatgaa
    26761 gtattggctg caatgggatt atcagacaaa tctattgaag gtagtataag attatcattt
    26821 ggggctacta caactaaaga agatatagcg aggtttaaag aaatatttat catcatttat
    26881 gaggaaatta aggagttgct aaaataatga agtatgatca cttgcttgtt agatacgggg
    26941 agttaacatt aaagggttca aatagaaaga aatttgtaaa tcaattaaga aataatgtaa
    27001 ataagtcatt aaaaggactt gatgggtttg tcgttaaagg caaacgagat cgtatgtata
    27061 ttgaacttga agaccatgca gatataaatg aaataacata tcgattatca aaaattttcg
    27121 gtattaaatc tattagtcca gtattaaaag tagaaaaaac aatagaggca ataagtgcag
    27181 cggcaattaa atttgcgcag caatttgaag aaaacagcac atttaaaatt gatgtgaagc
    27241 gtgccgataa aaatttccca atggatacgt atgaattaca gcgtgaattg ggtggtgcag
    27301 tattgaagca cttcgacaat atttcagtga atgtcaaacg tccagatcat gaaattcgag
    27361 tggaagttag attagatgca atttatatgt atgaagaagt tgttccgggt tcaggtggat
    27421 taccagttgg tactggtggt aagacgttac taatgctttc aggcggtata gactcaccag
    27481 ttgctgggat ggaagtgatg agacgtggcg taacaattga agcgattcat ttccatagtc
    27541 caccatttac aagtgatcaa gcaaaagaaa aagttattga attgacacgt attttagctg
    27601 aacgtgttgg accaattaaa ttgcatattg taccatttac agaattgcaa aaacaggtaa
    27661 ataaagttgt acatccaaga tatacaatga cttcaacgag acgtatgatg atgcgtgttg
    27721 ctgataaatt agtacatcaa ataggggctt tagctattgt aaatggtgaa aacctagggc
    27781 aggtagccag tcaaacactt catagcatgt atgcaattaa taatgtaact tctactcctg
    27841 tattacgtcc tttattaact tacgataaag aagaaattat tattaaatcg aaagaaattg
    27901 gtacatttga aacatctatt caaccatttg aagattgttg tacaattttc acccctaaaa
    27961 atccagtaac cgaaccaaac tttgataagg tagtccaata tgaaagtgtc tttgattttg
    28021 aagagatgat taatcgtgct gttgaaaata ttgaaacact tgaaataact agtgattata
    28081 aaactattaa agaacagcaa acaaaccaat taataaacga ctttttataa ataaaatcct
    28141 agagtaaatt taaacataag gggatgttaa actatggatt tgaacttaac gatgattata
    28201 atcataattt tatttggttt tatcgcggcg tttatagatt cggttgtagg gggtggcggt
    28261 ttaatttcta cgccagcatt attagcaatc ggtctaccac catctgtggc tttaggtaca
    28321 aataaattgg caagttcgtt tggttcttta actagtacga taaagtttat aaggtccggt
    28381 aaagtggact tatatgttgt tgccaaatta tttggttttg tatttttggc atctgcatgt
    28441 ggcgcatata ttgcaacgat ggttccgtca caaatattga aacctttaat catcattgca
    28501 ctttcgtcgg tgtttatatt cacattactt aaaaaagatt ggggcaatac acgcacgttt
    28561 actcaattta catttaagaa agccataata tttgcagcac tttttatatt aatcggcttt
    28621 tatgatggat ttgtaggtgg gggaacaggt tcatttatgc tttttgtatt gttagtcttt
    28681 ggttttgatt ttttaagtgc agcaggaaat gctaaagttt tgaactttgc ttctaatata
    28741 ggtgcgcttg tattatttat ggtattagga caagtagatt atgtaatagg tttaattatg
    28801 gctataagta tgattgctgg gtcatatgct ggggctcatt ttgctattaa acaaggtgtt
    28861 ggttatgtaa aagtattatt tattattatt actgctattt taattttaaa aaatgctttt
    28921 gattatattc aacaatttgt ataaaattaa atttgctttt gattaaagca tgaaaattgt
    28981 aatcaaacca taaattgtcg tatgatgtag ttagaatttt aaaatgcagg aggtcaagta
    29041 tatgactgaa ataacattca aaggtggacc aatccactta aaaggtcaac aaattaatga
    29101 aggtgatttt gcacctgatt ttacagtgtt agataatgac ttaaatcaag taacattagc
    29161 agattatgct ggtaaaaaga aattaattag tgtggtacca tcaattgata caggtgtttg
    29221 tgatcagcag actcgcaaat tcaactctga tgcttctaaa gaagagggga ttgtgcttac
    29281 aatttcagca gacttaccat tcgcacaaaa aagatggtgc gcttcagcag gtttagacaa
    29341 tgtcattaca ttaagtgacc accgtgactt atcatttggt gaaaactatg gcgttgttat
    29401 ggaagaactt cgcttattag ctcgtgcagt atttgtatta gatgcagata ataaagttgt
    29461 ttataaagaa atcgttagtg aaggtactga tttcccagat tttgatgctg ctttagctgc
    29521 atacaaaaat atttaatcat taaagagata aatcttaaaa tgtatacatc gtgtccatcg
    29581 ttgtcaacag cattaaaata gaattgtttt ctatgattgc taagacctat gggcactttt
    29641 tattggagag ggacgaatat ggcagaacaa caaacaatta tggaacgctt gtttcataca
    29701 ttagatgaaa aagctaaaac attaaataat gaaaatggcc aaagttttat tgaaaatctt
    29761 gggctagcaa tggaacaagt atataccaat gaaagaggat tgttagaaca atcgacgtta
    29821 caagatcgac gtaaagcatt ccaatttgca tatttaagtt taatgcagga agaaaagata
    29881 caagcaaatc atcaaattac accagattca attggattga tactaggatt tttagttgag
    29941 cgttttatga acaaccaaga agaattacat attgttgata ttgcaagtgg tgccggtcat
    30001 ttaagtgcta ctgtaaaaga agtgttacct gaaattgcgg ttatgcatca tttaattgaa
    30061 gttgatccag ttttatcacg tgttagtgta catttagcaa acttcttaga aattcctttc
    30121 gatgtgtatc ctcaagatgc catcatgcca ctaccattag aagaagcaga tatcgttatt
    30181 ggtgattttc cagtaggcta ttatccaatt gatgaaagaa gtaaggagtt taagctaggt
    30241 tttgaagaag gacatagtta ttcacattat ttattaatag aacaagcaat aaatgcatta
    30301 aaagatgctg gatatgcctt tctagtggta ccaagtaata tttttacagg tgaacatgta
    30361 aaacagcttg aaaaatatat tgcaacagag acagagatgc aagcattttt aaatttacca
    30421 ccaactttat ttaaaaatga aaaagcgcga aaatctatat taattttaca aaagaaaaaa
    30481 tcgggtgaaa caaagccagt tgaagtatta ttggcaaata ttcctgattt caaaattcct
    30541 tcacaatttc aaggatttat gacagagtta aatcagtgga tggacacaaa tcgtcctaaa
    30601 aaataatctg taatagtata gtcataaaac tgtatgaatg ataaaatgaa aaatgaaata
    30661 gtataaaaag caaacgatgg aggatcatta cttatgtcaa aattaatctt ggctatcaat
    30721 gctggtagtt catcattaaa atttcaatta attagaatgc ctgaagagga attagtaaca
    30781 aaaggtttag tagaaagaat cggattgaag gattcaattt ttacaattga agtcaacggg
    30841 gaaaaagtta aaacagtaca agatatcaag gatcacgttg aggcagttga tatcatgttg
    30901 gatgctttta aagcgcacaa tattattaat gatattaacg atatcgatgg aacaggtcac
    30961 cgtgttgtac atggtggtga aaaattccct gaatcagtag ctattactga tgaggttgaa
    31021 aaggaaattg aagaattaag cgaattagca ccgcttcaca acccagctaa cttgatgggt
    31081 attcgtgctt tccgtaaatt attaccaaat atccctcatg tggcaatttt tgatacagca
    31141 ttccatcaaa caatgcctga aaaagcatat ctatatagct tgccatatca ttattataaa
    31201 gattatggca ttcgtaagta tggtttccat ggtacaagcc ataaatttgt atcacaaaga
    31261 gcggcagaaa tgttagataa gccaattgaa gatttacgta ttatttcatg tcacattggt
    31321 aatggagcat caattgcagc tatcgatggc ggtaaatcta ttgatacatc aatgggcttc
    31381 acaccacttg ctggtgtaac aatgggtact cgttctggta atattgaccc tgctttaatt
    31441 ccatttatta tggaaaaaac aggtaaaact gctgaacaag tattggaaat cttaaacaaa
    31501 gaatcaggtt tattaggatt atcaggtaca tctagtgatt tacgtgactt atcagaagaa
    31561 gctgaatcag gtaaagcacg ttcacaaatg gctttagatg tatttgcttc taaaattcac
    31621 aaatatattg gatcatatgc tgcgagaatg catggtgttg atgttatcgt atttacagca
    31681 ggtatcggtg aaaactcagt agaaattcgt gcgaaagttc ttgaaggctt agaatttatg
    31741 ggtgtttact gggatcctaa gaaaaatgaa aacttactac gtggtaaaga aggatttatt
    31801 aactatcctc attcaccagt taaagttgtc gttattccaa cagatgaaga aagtatgatt
    31861 gcccgtgatg ttatgacatt cggtggttta aaataatttt taaataaata ttaagcgtct
    31921 atctccgcta caattatatg taggaggtag acgctttttg tattgtttga aaattaacta
    31981 gtgtaatgtc tcttttcacg aatatcttaa agaattaagt agtcgaagaa gattatgaaa
    32041 acattattaa gtgagtttta tttataaaca ataaaaaacc aagtgcagaa agttgcactt
    32101 ggttcataat gatcaagtag ttatctaatg atcatctata tttaattttg atatttttca
    32161 cgtaattgag ttgttgcaac ttgtggttgg aagtctgctg gtaactcttc agtacgaaca
    32221 actaacacgt cacatggcgc atgacgaacg atagattctg atacagaacc aacaataaat
    32281 ctttccacgg catttaagcc tgatgtacca ctcataatca agtctgcatt aatttcatgt
    32341 gcaagctttt taggaatgat agatttagga gagccaaact ctagacgcgt ttctacatct
    32401 ttaacaccag cgttagtagc tacttcttta taaccattta ataattcttc tgcaaaatgc
    32461 ttagattttt cagtaaattg agcatcataa acttcataag aagaatacgt tcttgaatca
    32521 attacattca caattgttaa cttcgcatcg ttacgtttag caacaccaac tgctctgtta
    32581 aatgcccatt ccgcttcatg tgaaccgtca actgcgatta aaatattttt gtaagtaatc
    32641 atagtaagtg cctccttgtt ctttcttaat tatattttac cacaattttc ggaaaagtca
    32701 gtgtaataac ctttacaatt ttgtggattt ttgattgcgc tttcaaaatg ttcgcgttaa
    32761 gatgattatg gaggtggatg gtatgaaaat tggtatacca agggagatta aaaataatga
    32821 aaatcgtgtt ggtttatcac caagtggtgt gcacgcttta gtagaaagtg ggcatactgt
    32881 tttagtggaa acaaatgcgg gttcaggatc attctttgaa gatgtagatt acaaagaagc
    32941 aggtgctgag attgttgctg aacaagcaaa agtttgggat gtggatatgg ttattaaagt
    33001 taaagaacca cttgaatctg aatatccata ttttaaagaa gggcttgtat tattcactta
    33061 tcttcattta gcaaatgaag aaaaattaac acaagctttg atagatagaa aagtaattag
    33121 tattgcatat gagactgtgc agttaccaga ccgatcttta ccattgttat caccaatgag
    33181 tgaggtagca ggaagaatgt cagctcaagt tggcgcagag ttcctacaaa aacttaatgg
    33241 tggtatggga attctactag gtggtgtccc aggagtacct aagggtaaag taactattat
    33301 cggtggtggt caagcaggaa caaatgcagc taaaattgca ctaggactag gtgcagatgt
    33361 tacgatttta gatgttaatc caaagcgttt acaacaatta gatgatttat tcggtggacg
    33421 tgtacataca attatgtcaa atccgttgaa tattgagttg tatgttaaac aaagtgattt
    33481 agtaataggt gcagttttaa ttccaggtgc taaagcgcca agacttgtaa cagaagacat
    33541 gattaaacaa atgaaaaatg ggtcagttat tattgacatt gctattgatc aaggcggtat
    33601 ttttgaaaca actgataaaa ttacgacaca tgatgatcct acatatatta agcatggtgt
    33661 ggttcattat gcagttgcaa atatgccagg tgcagtaccg cgtacttcga cgttagcttt
    33721 aaataatgct acgctacctt atgcgctcat gctagctaat aaagggtata gagaagcatt
    33781 taaatcaaat caaccattat cattaggttt aaatacttac aaaggtcacg taaccaataa
    33841 aggcgttgca gaggcatttg aaatggaata taaatctgta gaagaagcat tacaattata
    33901 atttgaattt gatgaaaagg gaatggatta cagcgtacta aagaatgacg ttatcatctc
    33961 cttggttatt aaaattaaat aatgataaat taaagctaga aacaatgcga agaaatcatc
    34021 gaacatgaat gatattgatt tgattcatta catgttactg atgaacatac ctgcatatgt
    34081 gtttctagct tgtttgttac atattgcatt tagacttgga taaaaattta tgtcttaggc
    34141 gctacaatgt tatattggca gtgtttgact gaatgagaat gcgcttgtaa caagcttttc
    34201 atttttatcc cactccttat ttttcgtaat gtgttaatac ttcatatcct tcattagtga
    34261 caagtatgtc atcttcaatt cttacacctg caacaccagg tacataaata cctggttcga
    34321 ttgtaataac catgccagct tctaacaaat tagaattagt acttgaaaca tcttgatatt
    34381 catgttcttg taatcctagg ccatgaccta agcgatgagg gaaatattca ccataacctt
    34441 tttctgaaat aatatttcta gcgatatgat cgatatcttt taatggtatt ccaggtttaa
    34501 ttgcttggat tgcagatgtt tctgcttcta atacaatatt ataaatttct tgtgcttctt
    34561 tgctaggttc accaaattta atagtacgtg tcatatcgct acaataatgc tcataaatta
    34621 cacctaaatc aaatagtaca tattcattgc tttttaatct gcgatctcct ggtgtgccat
    34681 gaggtgatgc ggcatgatct ccaaataaaa ccatcgtatc aaaactcatt tcattgacgc
    34741 catattgttt gatagtttgc tcaatatggt tgactacttc acattcagtc acaccttctt
    34801 ttaaataaga aacacctatt tcgatacact tatctgctaa ctcagcagct ttacgtattt
    34861 tgctaatttc atcttcggat ttaatatttc tcaattgttt gattgttaaa tcaacatctc
    34921 cgaatgaatt gacattgaaa ccagagatta attgtttttg gcgtgctact gttaagtgct
    34981 cgctttcaat tagtaattta ttgattgttt gaggataaag tgaaaaaggg ttttcagtgt
    35041 ctaaatatcc aacgatttca cctgtgaaag gtgatgcttt gacttcttcg acttccattt
    35101 ttggacaaaa tagtacttgt ttaccatctt tcttaatcaa taatgcaaat aatctttcat
    35161 ggggttcgct acggtatcca gtaaaataat atacattcaa cggtgttgta atccatgctg
    35221 catcagcttg ttgattgttc aattcgtcta ttatttttga tatttttgtc attttataaa
    35281 cactcctcaa cataatatta cttccatttt atgccaatta ttattagatt tcaaaatcga
    35341 gctaatacat taactagatt ttcaattttt aaaatttgag aatttgttat aattaagtgg
    35401 caaaagggta ctaaatttat atagtaattg ataggaggat gctcaaatga agttatcatt
    35461 tcatggtcaa tcaacgattt atttagaagg gaacaacaaa aaggttatcg tagatccgtt
    35521 tatttcaaat aaccctaagt gtgatttgaa tattgaaaca gtgcaggtag actatatcgt
    35581 tttaacgcac ggtcattttg atcattttgg tgatgttgtg gaactagcaa agaaaacagg
    35641 agcaacagta attggaagtg cagaaatggc tgactatctt tcttcatatc acggtgttga
    35701 aaatgttcat ggtatgaata ttggtggtaa agcaaacttt gatttcggaa gcgttaaatt
    35761 tgttcaagca tttcatagtt caagttttac acatgaaaat ggtattccag tttatttagg
    35821 tatgccaatg ggtatcgtgt ttgaagttga aggaaaaaca atttatcata ctggtgatac
    35881 aggtctgttt agtgacatgt cacttattgc gaaacgtcat ccagttgatg tatgttttgt
    35941 accaattgga gataatttta cgatgggtat tgatgatgca agttatgcta ttaatgaatt
    36001 tattaaacca aaaatttctg taccgattca ttatgataca ttcccattaa tcgaacaaga
    36061 tccacaacaa tttaaagatg ctgttaatgt aggggacgtg caaattttaa aaccaggtga
    36121 atctgttcag ttttaaatag aatatagatt ttacactaaa aaacacctcg ctaatctatg
    36181 tggaagttat cgtctgcata tttttagtgg gtgtttttat attctactga ttcagttacg
    36241 acgtggacgg gggacatcag tggttcttta tcatttctga cctactctcg taattttagt
    36301 tttatacaat tagacattga ttataaattt actacgatat aaaaaatcca gaaccacagt
    36361 cataatcgtt tcttgtattt actcagtcgt ttgagactgc atcatgattg aaagattatt
    36421 tgtgattctg gataaaaatt ttatttaacg attaatacag ggatagtcgc acgttttgct
    36481 actttgtggc tgacacttcc aagtacaaat ttctttttgt cttctgcttt acggttactt
    36541 aaaacaacaa tttcgtattt accgctatta gcatgtttta atagttcttc ttttgcatta
    36601 ccacgaacaa ttatttggtc ataatcaata ccataatctt ctaaagcttt gcgtgttttt
    36661 tccaatcgct tgcttcgttc ttcagtaagt ttgtttaaat gaacacctgc tttaattgat
    36721 gcttgagcat cttgttcgct gattgcgttt aaaactgtta cgactgtacc ttcgccagct
    36781 aatttagaca cttcttttag tgctttttca ttttttaact gagtgtctac accaagtaat
    36841 atatttttat acattagtac atccctcctc acttacattc taataaataa taatggattt
    36901 ttccatttaa aattaaagat tttgtgatct ttgtgtttgt tgtatatttt caaaaaataa
    36961 aataggtgaa acgatcatta tttcatttca cttcaatgct ttttatccaa aataaagtta
    37021 taatgtagtt aatactgttc gaaaagaaga ggtaaagaat gacaaaacat gaacaaattt
    37081 tagattatat tgaatcgtta tctataggct ctaaaatttc ggtgcgtaaa atcgctaaat
    37141 ttttaaacgt atctgaaggg acagcatatc gtgcaattaa agatgctgat aaaatgggca
    37201 tggtcgcaac gatagatcgt gttggtacgg ttcgaatcga aaaacgtaat cgtaatgaaa
    37261 ttgaacattt aacttttaat gaaattgtca atattattga tggccaagtg ttaggtggca
    37321 acaaaggtat cactaagatg gtatctaaat ttgcaattgg agcaatggaa ctcaaagata
    37381 ttttaaagta tattggtcca aagacattat tgattgtcgg aaatcgtgaa gatgtgcaga
    37441 ttgaagcttt aaaaagaggt acggctatat taattactgg tggatttaag ccatctaata
    37501 aagttattaa ttttgcgaat gaacatgatt tacctgttct atcatcaagt tatgatacat
    37561 tcctagttgc aaatattatc aacaaagcac tttttaatca gaaaatacga aaagatatct
    37621 taatagttca agatataatg acgccattag atgatttatc tgtgctgttt gatacgatga
    37681 aaatagcaga ttataagcgt atggcgaatc gaactggcca tacaagattt ccggtagtca
    37741 atgagtcgta taaattagta ggtattgtaa caagtcgaga gatgattaat acaaaggacg
    37801 acgacgaaat agataaagtg atgacgcgaa atcctattta tgtaaatgct atgagtacag
    37861 tagcaagttg tgcgcatatg atgatttggg aaggtattga attgattcct gtcgtttcga
    37921 gtaataaaaa gacagtgggt gtgattaata gacaagatgt tttaaagtct atgcaacttt
    37981 taggaagaca gcctcagatg ggtgaaacaa ttaacgatca aatcgccaaa tatataacaa
    38041 tgaatcaaga tggaattaca gtagaagttt caccactttt aatcaatcat tacggaacag
    38101 taagtaaagc ggcatttgta agtattatag aagaaacaat acaatatgaa atgcgtaaat
    38161 ttaaaaaagg taatgtcatg atagagaact taaatatcgt gtatattaaa acagtaccta
    38221 ttgaatcaca tataacagtg cgctttggca tactagacgt cggaagaaac ttcgccaaaa
    38281 tagaagtgaa catgcatagt caaaacgaca aagtagcgag cgctctcgtg atatgccaaa
    38341 tgtttgacga agtataggaa cttagcagag aaaataggca taagatagat ttcgcacaaa
    38401 cgttggggcc ccaccccaac ttgcattgtt tgtagaaatt aggggtccaa tttctctgtg
    38461 ttggggcccc tgaactaaat agtatgaaaa tatatatata tctaaataat taaaacgggc
    38521 aaatagttta atatggtagt aacaattttt ataatttaga tattttgata tagactaatg
    38581 cttaaattaa gcaacttttc taaaaaaggg agtatgaaaa tgattagtac tttgaatgaa
    38641 attatgaaat gtatcgaaga caacgataca attattatac acagacatgt tagaccagat
    38701 cctgatgcat atggttcaca acttggttta aaatactata ttcagcagaa atttccgcaa
    38761 aagcaagtat ttgcagtggg tgaagcggaa tcatcattaa gttttattgg agagttagat
    38821 aacattgatg ataaaacata tcaagatgcg cttgtaattg tatgtgatac tgccaatgct
    38881 ccacgaattg acgatgaacg ttatagtaca ggtcgtaaac ttattaaaat agatcatcat
    38941 cctgcagttg atcagtatgg tgatattaat ttagttaata cgaacgcgtc atctacaagt
    39001 gaaatcattt acgatttaat ctcacatttt aatgatgaag caattgttaa taaagatatc
    39061 gcgagcgttt tatatcttgg tatcgtcggt gatactgggc gattcctttt taacaatacc
    39121 tcagaacata ctatggaaat tgctggaaag ttaattgggc atgatattga tcataatgcg
    39181 cttttaaata aaatgatgga gaaggaccca aaaatgttgc cgtttcaagg ttatgtttta
    39241 caacatttcg aacttatgga tgatggattc tgccaagtta aaataactga agatgtattg
    39301 gagcaattcg gtattcagcc aaatgaagca tctcagtttg ttaatacaat tgctgacatc
    39361 aaaggcttga aaatatgggt atttgcagtc gatgaaggta atgaaatcag atgtcgatta
    39421 cgttctaaag ggcaattgat tattaatgat attgcgcaag attttggtgg cggtggtcat
    39481 ccgaatgcgt caggagtttc agtggacagc tgggatgaat ttgagcaact tgctacagct
    39541 ttacgcacaa aacttaacta atagaaaggg gccattcaat catggtggca tatttaaata
    39601 ttcatacggc ttatgatttg ttaaattcaa gcttaaaaat agaagatgcc gtaagacttg
    39661 ctgtgtctga aaatgttgat gcacttgcca taactgacac caatgtattg tatggttttc
    39721 ctaaatttta tgatgcatgt atagcaaata acattaaacc gatttttggt atgacaatat
    39781 atgtgacaaa tggattaaat acagtcgaaa cagttgttct agctaaaaat aatgatggat
    39841 taaaagattt gtatcaacta tcatcggaaa taaaaatgaa tgcattagaa catgtgtcgt
    39901 ttgaattatt aaaacgattt tctaacaata tgattatcat ttttaaaaaa gtcggtgatc
    39961 aacatcgtga tattgtacaa gtgtttgaaa cccataatga cacatatatg gaccacctta
    40021 gtatttcgat tcaaggtaga aaacatgttt ggattcaaaa tgtttgttac caaacacgtc
    40081 aagatgccga tacgatttct gcattagcag ctattagaga caatacaaaa ttagacttaa
    40141 ttcatgatca agaagatttt ggtgcacatt ttttaactga aaaggaaatt aatcaattag
    40201 atattaacca agaatattta acgcaggttg atgttatagc tcaaaagtgt gatgcagaat
    40261 taaaatatca tcaatctcta cttcctcaat atgagacacc taatgatgaa tcagctaaaa
    40321 aatatttgtg gcgtgtctta gttacacaat tgaaaaaatt agaacttaat tatgacgtct
    40381 atttagagcg attgaaatat gagtataaag ttattactaa tatgggtttt gaagattatt
    40441 tcttaatagt aagtgattta atccattatg cgaaaacgaa tgatgtgatg gtaggtcctg
    40501 gtcgtggttc ttcagctggc tcactggtca gttatttatt gggaattaca acgattgatc
    40561 ctattaaatt caatctatta tttgaacgtt ttttaaaccc agaacgtgta acaatgcctg
    40621 atattgatat tgactttgaa gatacacgcc gagaaagggt cattcagtac gtccaagaaa
    40681 aatatggcga gctacatgta tctggaattg tgactttcgg tcatctgctt gcaagagcag
    40741 ttgctagaga tgttggaaga attatggggt ttgatgaagt tacattaaat gaaatttcaa
    40801 gtttaatccc acataaatta ggaattacac ttgatgaagc atatcaaatt gacgatttta
    40861 aaaagtttgt acatcgaaac catcgacatg aacgctggtt cagtatttgt aaaaagttag
    40921 aaggtttacc aagacataca tctacacatg cggcaggaat tattattaat gaccatccat
    40981 tatatgaata tgccccttta acgaaagggg atacaggatt attaacgcaa tggacaatga
    41041 ctgaagccga acgtattggg ttattaaaaa tagattttct agggttgaga aacttatcga
    41101 ttattcatca aatcttaaca caagtcaaaa aagatttagg tattaatatt gatatcgaaa
    41161 agattccgtt tgatgatcaa aaagtgtttg aattgttgtc gcaaggagat acgactggca
    41221 tattccaatt agagtctgac ggtgtaagaa gtgtattaaa aaaattaaag ccggaacact
    41281 ttgaagatat tgttgctgta acttctttgt atagaccagg tccaatggaa gaaattccaa
    41341 cttacattac aagaagacat gatccaagca aagttcaata tttacatccg catttagaac
    41401 ctatattaaa aaatacttac ggtgttatta tttatcaaga gcaaattatg caaatagcga
    41461 gcacatttgc aaacttcagt tatggtgaag cggatatttt aagaagagca atgagtaaaa
    41521 aaaatagagc tgttcttgaa agtgagcgtc aacattttat agaaggtgca aagcaaaatg
    41581 gttatcacga agacattagt aagcaaatat ttgatttgat tctgaaattt gctgattatg
    41641 gttttcctag agcacatgct gtcagctatt ctaaaattgc atacattatg agctttttaa
    41701 aagtccatta tccaaattat ttttacgcaa atattttaag taatgttatt ggaagtgaga
    41761 agaaaactgc tcaaatgata gaagaagcaa aaaaacaagg tatcactata ttgccaccga
    41821 acattaacga aagtcattgg ttttataaac cttcccaaga aggcatttat ttatcaattg
    41881 gtacaattaa aggtgttggt tatcaaagtg tgaaagtgat tgttgatgaa cgttatcaga
    41941 acggcaaatt taaagatttc tttgattttg ctagacgtat accgaagaga gtcaaaacga
    42001 gaaagttact tgaagcactg attttagtgg gagcgtttga tgcttttggt aaaacacgtt
    42061 caacgttgtt gcaagctatt gatcaagtgt tggatggcga tttaaacatt gaacaagatg
    42121 gttttttatt tgatatttta acgccaaaac agatgtatga agataaagaa gaattgcctg
    42181 atgcacttat tagtcagtac gaaaaagaat atttaggatt ttatgtttcg caacacccag
    42241 tagataaaaa gtttgttgcc aaacaatatt taacgatatt taaattgagt aacgcgcaga
    42301 attataaacc tatattagta cagtttgata aagttaaaca aattcgaact aaaaatggtc
    42361 aaaatatggc attcgtcaca ttaaatgatg gcattgaaac tttagatggt gtgattttcc
    42421 ctaatcagtt taaaaagtac gaagagttgt tatcacataa tgacttgttt atagttagcg
    42481 ggaaatttga ccatagaaag caacaacgtc aactaattat aaatgagatt cagacattag
    42541 ccacttttga agaacaaaaa ttagcatttg ccaaacaaat tataattaga aataaatcac
    42601 aaatagatat gtttgaagag atgattaaag ctacgaaaga gaatgctaat gatgttgtgt
    42661 tatcctttta tgatgaaacg attaaacaaa tgactacttt aggctatatt aatcaaaaag
    42721 atagtatgtt taataatttt atacaatcct ttaaccctag tgatattagg cttatataac
    42781 ttttatcaac taataaatta tgatatagta aactgatggt tagatatttt ttaaccatca
    42841 gtttgcgttt atattagttt tttatgctta ttatttttat gagtttcact ttacattatt
    42901 gattaatcca taagaataat tagcacaaaa agcagtatac ataaattgag taaagaattt
    42961 tgtcgatata tcgtgcggaa aaataataac tttttgatat attggttttt gctatccatt
    43021 ataataatta aaggtatatc tttagatagt atggtttaat gtatctttat aaatattatt
    43081 ttattatcgt ttatcgctat ttgtgttaca taatcaaaac cataaattct tactcattca
    43141 gatttaccca atatttttac ttttataatg taatgcgttt tatccaagtt attttttaaa
    43201 aataaatatt gaattagggg ctgatttcat gtcattaaga gatgaagcat tggaaatgca
    43261 caaacgtaat caaggtaaat tagaagttaa accaaatgta aaagttacta ataaagagga
    43321 attaagttta gcatactcac ctggcgttgc tgaaccgtgt aaagatattt atgaagataa
    43381 aagaaaagta tatgattaca caattaaagg aaatacagtt gcagttatta ctgatggaac
    43441 agcggtatta ggtttaggta acattggacc tgaagcaagt attcctgtaa tggaaggtaa
    43501 agcagtatta ttcaaaagct tcgctggtat caatggggtg cctattgcgt taaatacaac
    43561 tgataccgaa gaaatcatta aaacagttaa gttgttagaa cctaattatg gtggtattaa
    43621 tttagaggat atttcggcac cacgttgttt tgaaattgaa gaacgattga aaaaagaaac
    43681 taatattccg gtattccatg acgatcaaca tggtacagca attgtaacat tggcaggttt
    43741 ggtaaatgca ttgagagttg ttaacaaaga tattgctaaa ataaaagttg tactaaatgg
    43801 tgctggtgca gcaggaatag ccattgttaa attactatac gcgtatggtg taagaaatat
    43861 ggttatgtgt gactcaagag gcgcaatttt tgaaggacgt tcatatggta tgaatcctac
    43921 gaaagatgtt gtagcaaaat ggacaaataa agataagatt gaagggtctt tagaagaagt
    43981 cgtaaaagat gcagatgtat ttatcggggt ttctgtagct aatgcgctgt cacaagatat
    44041 ggttaagagt atggcagata atccaattat atttgcaatg gctaatccaa atcctgaaat
    44101 aatacctgat gatgccaaag cggcaggtgc acgagttgtt ggtacaggac gttcagacta
    44161 tcctaaccaa attaataatg tattagcttt ccctggtatt tttagaggtg cattagaggt
    44221 tgaagctaca catataaatg aagaaatgaa aaaggcagct gtagaagcga ttgctgattt
    44281 aatcgatagt tctgaattaa atgaagacta ctgtatccca ggaccgtttg ataaacgtgt
    44341 agcgccatca gttgctcgta atgttgctaa agcggcaatg gaatctggag tagctaggat
    44401 tgaagttgat ccgcaagatg tgtatgataa aacaatgaaa cttacagatt tacaataaaa
    44461 ataattaacg tttaattaaa ttgcttaaaa gttagcaata aagagtcatt aaaatgatga
    44521 aaatgaaagt ttatgataaa cattcaacag tcaaacgaat ataaatcaaa taaatttaaa
    44581 cccgttttta actggtcaag ttcagtttaa ggctctaaat ggttagaaca gaggttattt
    44641 ggaggttttc ctatgtttaa agattttttt aatcgaacaa agaaaaagaa atatcttaca
    44701 gtacaagact ctaaaaataa tgatgtgcct gcaggtatta tgactaagtg tccaaagtgt
    44761 aagaaaatta tgtacacaaa agaattagct gaaaatttaa atgtgtgctt taattgtgat
    44821 catcatattg ctttaactgc gtataaacgt atagaagcaa tttctgatga aggatcattt
    44881 acagaattcg ataagggaat gacctctgcg aatccattag attttccaag ttatttagaa
    44941 aaaattgaaa aggaccaaca aaagacaggt cttaaagaag cagttgtgac tggtacagca
    45001 caactagatg gtatgaaatt tggcgttgct gtcatggatt cacgttttag aatgggaagt
    45061 atgggatcgg ttatcggtga aaagatatgt cgcatcattg attactgcac tgagaaccgt
    45121 ttaccattta ttcttttctc tgcaagtggt ggtgcacgta tgcaagaagg tattatttcc
    45181 ttgatgcaaa tgggtaaaac cagtgtatct ttaaaacgtc attctgacgc tggactatta
    45241 tatatatcat atttaacaca tccaactact ggtggtgtat ctgcaagttt tgcatcagtt
    45301 ggtgatataa atttaagtga gccaaaagcg ttgataggtt ttgcaggtcg tcgagttatt
    45361 gaacagacaa taaacgaaaa attgccagat gatttccaaa ctgcagaatt tttattagag
    45421 catggacaat tggataaagt tgtacatcgt aatgatatgc gtcaaacatt gtctgaaatt
    45481 ctaaaaatcc atcaagaggt gactaaataa tgttagattt tgaaaaacca ctttttgaaa
    45541 ttcgaaataa aattgaatct ttaaaagaat ctcaagataa aaatgatgtg gatttacaag
    45601 aagaaattga catgcttgaa gcgtcattgg aacgagaaac taaaaaaata tatacaaatc
    45661 taaaaccatg ggatcgtgtg caaattgcgc gtttgcaaga aagacctacg accctagatt
    45721 atattccata tatctttgat tcgtttatgg aactacatgg tgatcgtaat tttagagatg
    45781 atccagcaat gattggtggt attggctttt taaatggtcg tgctgttaca gttattggac
    45841 aacaacgtgg aaaagataca aaagataata tttatcgaaa ttttggtatg gcgcatccag
    45901 aaggttatcg aaaagcatta cgtttaatga aacaagctga aaaattcaat cgtcctatct
    45961 ttacatttat agatacaaaa ggtgcatatc ctggtaaagc tgctgaagaa cgtggacaaa
    46021 gtgaatctat cgcaacaaat ttgattgaga tggcttcatt aaaagtacca gttattgcga
    46081 ttgtcattgg tgaaggtggc agtggaggtg ctctaggtat tggtattgcc aataaagtat
    46141 tgatgttaga gaatagtact tactctgtta tatctcctga aggtgcagcg gcattattat
    46201 ggaaagacag taatttggct aaaattgcag ctgaaacaat gaaaattact gcccatgata
    46261 ttaagcaatt aggtattata gatgatgtca tttctgaacc acttggcggt gcacataaag
    46321 atattgaaca gcaagcttta gctattaaat cagcgtttgt tgcacagtta gattcacttg
    46381 agtcattatc acgtgatgaa attgctaatg atcgctttga aaaattcaga aatatcggtt
    46441 cttatataga ataatcaact tgagcatttt tatgttaaat cgatactggg ttttaccata
    46501 aattgaagta cattaaaaca ataatttaat atttagatac tgaattttaa ctaagattag
    46561 tagtcaaaat tgtggctact aatctttttt taattaagtt aaaataaaat tcaatattta
    46621 aaacgtttac atcaattcaa tacattagtt ttgatggaat gacatatcaa tttgtggtaa
    46681 tttagagtta aagataaatc agttatagaa aggtatgtcg tcatgaagaa aattgcagtt
    46741 ttaactagtg gtggagattc acctggaatg aatgctgccg taagagcagt tgttcgtaca
    46801 gcaatttaca atgaaattga agtttatggt gtgtatcatg gttaccaagg attgttaaat
    46861 gatgatattc ataaacttga attaggatca gttggggata cgattcagcg tggaggtaca
    46921 ttcttgtatt cagcaagatg tccagagttt aaggagcaag aagtacgtaa agttgcaatc
    46981 gaaaacttac gtaaaagagg gattgagggc cttgtagtta ttggtggtga cggtagttat
    47041 cgcggtgcac aacgcatcag tgaggaatgt aaagaaattc aaactatcgg tattcctggt
    47101 acgattgaca atgatatcaa tggtactgat tttacaattg gatttgacac agcattaaat
    47161 acgattattg gcttagtcga caaaattaga gatactgcgt caagtcacgc acgaacattt
    47221 atcattgaag caatgggccg tgattgtgga gatctagcat tatgggctgg attatcagtt
    47281 ggtgctgaga caattgtagt tccagaagtg aaaacagata ttaaagaaat agctgataaa
    47341 attgaacaag gtattaaacg tggtaagaaa cactcaatcg ttcttgtagc agaaggttgt
    47401 atgactgcgc aagattgtca aaaagaatta tcacaataca tcaatgttga taatagagtg
    47461 tctgtgttag gtcacgttca acgtggtggt agcccaacag gtgcggatag agttttagca
    47521 tcacgtttag gtggatatgc ggtagactta ttaatgcaag gtgaaacagc taagggtgtt
    47581 ggaattaaga acaataaaat tgtagcaaca tcttttgatg aaatttttga tggtaaagat
    47641 cataaatttg attatagtct atatgaactt gctaacaagt tatctatata agatttcagg
    47701 aggaattata aaatgagaaa aactaaaatt gtatgtacaa ttggaccagc ttcagaatca
    47761 gaagaaatga ttgagaaatt aatcaatgct ggtatgaacg ttgcacgatt aaacttttca
    47821 catggtagtc atgaagagca taaaggtaga attgatacaa ttcgtaaagt agctaaaaga
    47881 ttagacaaaa ttgtagcaat tttattagat acaaaaggtc cagaaattcg tacgcataat
    47941 atgaaagacg gtatcattga acttgaacgt ggcaacgaag ttattgttag catgaatgaa
    48001 gttgaaggaa cacctgaaaa gttctcagta acatatgaaa acttaattaa cgatgttcaa
    48061 gtaggttcat acattttact tgatgatggc ttaattgaat tacaagttaa agatattgac
    48121 catgctaaaa aagaagttaa atgtgatatt ttaaactctg gtgagcttaa aaacaaaaaa
    48181 ggtgttaact tacctggcgt aagagtaagt ttacctggta ttacagaaaa agatgctgaa
    48241 gatatccgtt tcggtattaa agaaaatgtt gacttcattg cagcaagttt cgtacgtcgt
    48301 cctagtgatg ttttagaaat tcgtgaaatt ttagaagaac aaaaagctaa catttcagta
    48361 ttccctaaaa ttgaaaacca agaaggtatt gataatattg cggaaattct tgaagtgtct
    48421 gatggtttaa tggttgcacg tggtgacatg ggtgttgaaa ttccacctga aaaagtacca
    48481 atggttcaaa aagatttaat cagacaatgt aacaaattag gtaaaccagt tattacagct
    48541 acacaaatgt tagattctat gcaacgtaac ccacgtgcta cacgtgcaga agctagtgac
    48601 gttgccaacg caatctatga tggtacagat gcagtaatgt tatctggtga aactgctgct
    48661 ggtttatatc ctgaagaagc tgttaaaaca atgagaaata ttgctgtatc agctgaagca
    48721 gcccaagatt acaaaaagtt attgtcagat cgtactaaat tagttgaaac ttcattagtg
    48781 aatgctatcg gtatttcggt tgcacataca gctttaaact taaatgttaa agcaattgta
    48841 gctgctactg aaagtggttc aacggcacgt actatctcca aatatcgtcc acattcagac
    48901 attattgcgg tgactccaag tgaagaaact gcacgtcaat gttcaattgt ttggggagtt
    48961 caacctgtag ttaaaaaagg acgtaagagt acagatgcat tgttaaacaa tgcagttgca
    49021 acagctgttg aaactggtag agtatctaat ggtgatttaa tcattattac tgctggtgta
    49081 ccaactggtg aaactggaac tactaatatg atgaaaatcc acctagttgg tgacgaaatt
    49141 gctaatggtc aaggtattgg acgtggatca gttgttggta ctacgttagt tgctgaaact
    49201 gttaaagatt tagaaggtaa agatttatct gacaaagtta tcgttactaa ctcaatcgat
    49261 gaaacgtttg taccttatgt agaaaaagct ttaggcttaa ttacagaaga aaatggtatt
    49321 acatcaccaa gtgcaattgt tggtttagaa aaaggtattc caacagttgt aggtgtagaa
    49381 aaagctgtta aaaacataag caataacatg ttagttacga ttgatgctgc tcaaggtaaa
    49441 atctttgaag gatatgcaaa cgtactataa tttataaaaa aacgtctttc catttatcaa
    49501 caatggaaag gcgttttttg gttcatctgg tattttatga cgtaattaat aggttatttg
    49561 ataatgatag tgtatgaatg gcaatctata taaatgttta tatcttttat acatgtacat
    49621 tatcaccttc aaactttcac tcatattact ttggaaattt at
//
LOCUS       DALAGF010000005        48868 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.005, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000005 DALAGF010000000
VERSION     DALAGF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 48868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48868
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.005"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(189..755)
                     /locus_tag="O2692_000268"
     CDS             complement(189..755)
                     /locus_tag="O2692_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCZ4297918.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
     gene            complement(776..1645)
                     /locus_tag="O2692_000269"
     CDS             complement(776..1645)
                     /locus_tag="O2692_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCZ4297919.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKEVMSIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            complement(1792..2262)
                     /locus_tag="O2692_000270"
     CDS             complement(1792..2262)
                     /locus_tag="O2692_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HCZ4297920.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            2329..2946
                     /locus_tag="O2692_000271"
     CDS             2329..2946
                     /locus_tag="O2692_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCZ4297921.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            2939..3601
                     /locus_tag="O2692_000272"
     CDS             2939..3601
                     /locus_tag="O2692_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCZ4297922.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMINKIARIIQTYRQVD
                     TR"
     gene            complement(3717..4400)
                     /locus_tag="O2692_000273"
     CDS             complement(3717..4400)
                     /locus_tag="O2692_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ4297923.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(4650..5726)
                     /locus_tag="O2692_000274"
     CDS             complement(4650..5726)
                     /locus_tag="O2692_000274"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCZ4297924.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITRKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            complement(6061..7026)
                     /locus_tag="O2692_000275"
     CDS             complement(6061..7026)
                     /locus_tag="O2692_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCZ4297925.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            complement(7240..8877)
                     /locus_tag="O2692_000276"
     CDS             complement(7240..8877)
                     /locus_tag="O2692_000276"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCZ4297926.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAENRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            complement(9037..9915)
                     /gene="hchA"
                     /locus_tag="O2692_000277"
     CDS             complement(9037..9915)
                     /gene="hchA"
                     /locus_tag="O2692_000277"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCZ4297927.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            complement(10181..11368)
                     /locus_tag="O2692_000278"
     CDS             complement(10181..11368)
                     /locus_tag="O2692_000278"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCZ4297928.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWNNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            11539..12714
                     /locus_tag="O2692_000279"
     CDS             11539..12714
                     /locus_tag="O2692_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCZ4297929.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTFKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            complement(12996..14180)
                     /gene="tuf"
                     /locus_tag="O2692_000280"
     CDS             complement(12996..14180)
                     /gene="tuf"
                     /locus_tag="O2692_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCZ4297930.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(14397..16478)
                     /gene="fusA"
                     /locus_tag="O2692_000281"
     CDS             complement(14397..16478)
                     /gene="fusA"
                     /locus_tag="O2692_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCZ4297931.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            complement(16601..17071)
                     /gene="rpsG"
                     /locus_tag="O2692_000282"
     CDS             complement(16601..17071)
                     /gene="rpsG"
                     /locus_tag="O2692_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCZ4297932.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            complement(17137..17550)
                     /gene="rpsL"
                     /locus_tag="O2692_000283"
     CDS             complement(17137..17550)
                     /gene="rpsL"
                     /locus_tag="O2692_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCZ4297933.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            complement(17648..17902)
                     /locus_tag="O2692_000284"
     CDS             complement(17648..17902)
                     /locus_tag="O2692_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HCZ4297934.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            complement(18039..21635)
                     /gene="rpoC"
                     /locus_tag="O2692_000285"
     CDS             complement(18039..21635)
                     /gene="rpoC"
                     /locus_tag="O2692_000285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCZ4297935.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIHHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            complement(21799..25350)
                     /gene="rpoB"
                     /locus_tag="O2692_000286"
     CDS             complement(21799..25350)
                     /gene="rpoB"
                     /locus_tag="O2692_000286"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCZ4297936.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            complement(25565..26173)
                     /locus_tag="O2692_000287"
     CDS             complement(25565..26173)
                     /locus_tag="O2692_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ4297937.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            complement(26348..26716)
                     /gene="rplL"
                     /locus_tag="O2692_000288"
     CDS             complement(26348..26716)
                     /gene="rplL"
                     /locus_tag="O2692_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCZ4297938.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            complement(26758..27258)
                     /locus_tag="O2692_000289"
     CDS             complement(26758..27258)
                     /locus_tag="O2692_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCZ4297939.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     misc_feature    complement(27296..27442)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(27530..28222)
                     /gene="rplA"
                     /locus_tag="O2692_000290"
     CDS             complement(27530..28222)
                     /gene="rplA"
                     /locus_tag="O2692_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCZ4297940.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSVKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
     gene            complement(28430..28852)
                     /gene="rplK"
                     /locus_tag="O2692_000291"
     CDS             complement(28430..28852)
                     /gene="rplK"
                     /locus_tag="O2692_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCZ4297941.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            complement(29033..29581)
                     /gene="nusG"
                     /locus_tag="O2692_000292"
     CDS             complement(29033..29581)
                     /gene="nusG"
                     /locus_tag="O2692_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCZ4297942.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            complement(29594..29776)
                     /gene="secE"
                     /locus_tag="O2692_000293"
     CDS             complement(29594..29776)
                     /gene="secE"
                     /locus_tag="O2692_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCZ4297943.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFR"
     gene            complement(29832..29975)
                     /gene="rpmG"
                     /locus_tag="O2692_000294"
     CDS             complement(29832..29975)
                     /gene="rpmG"
                     /locus_tag="O2692_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCZ4297944.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            complement(30090..30659)
                     /locus_tag="O2692_000295"
     CDS             complement(30090..30659)
                     /locus_tag="O2692_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCZ4297945.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            complement(30740..31264)
                     /locus_tag="O2692_000296"
     CDS             complement(30740..31264)
                     /locus_tag="O2692_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCZ4297946.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            complement(31264..32010)
                     /gene="rlmB"
                     /locus_tag="O2692_000297"
     CDS             complement(31264..32010)
                     /gene="rlmB"
                     /locus_tag="O2692_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCZ4297947.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLLTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            complement(32018..32422)
                     /locus_tag="O2692_000298"
     CDS             complement(32018..32422)
                     /locus_tag="O2692_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCZ4297948.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            complement(32415..33815)
                     /locus_tag="O2692_000299"
     CDS             complement(32415..33815)
                     /locus_tag="O2692_000299"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCZ4297949.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQITTN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            complement(33799..34446)
                     /gene="cysE"
                     /locus_tag="O2692_000300"
     CDS             complement(33799..34446)
                     /gene="cysE"
                     /locus_tag="O2692_000300"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCZ4297950.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            complement(34869..36323)
                     /locus_tag="O2692_000301"
     CDS             complement(34869..36323)
                     /locus_tag="O2692_000301"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCZ4297951.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            complement(36880..37953)
                     /locus_tag="O2692_000302"
     CDS             complement(36880..37953)
                     /locus_tag="O2692_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCZ4297952.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            complement(37978..39342)
                     /gene="radA"
                     /locus_tag="O2692_000303"
     CDS             complement(37978..39342)
                     /gene="radA"
                     /locus_tag="O2692_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCZ4297953.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEVLSF
                     ALHS"
     gene            complement(39827..42283)
                     /locus_tag="O2692_000304"
     CDS             complement(39827..42283)
                     /locus_tag="O2692_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCZ4297954.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            complement(42297..43304)
                     /locus_tag="O2692_000305"
     CDS             complement(42297..43304)
                     /locus_tag="O2692_000305"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HCZ4297955.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            complement(43294..43860)
                     /locus_tag="O2692_000306"
     CDS             complement(43294..43860)
                     /locus_tag="O2692_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HCZ4297956.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            complement(43879..44340)
                     /locus_tag="O2692_000307"
     CDS             complement(43879..44340)
                     /locus_tag="O2692_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCZ4297957.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            44498..45712
                     /locus_tag="O2692_000308"
     CDS             44498..45712
                     /locus_tag="O2692_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCZ4297958.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            complement(45920..46480)
                     /gene="pdxT"
                     /locus_tag="O2692_000309"
     CDS             complement(45920..46480)
                     /gene="pdxT"
                     /locus_tag="O2692_000309"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HCZ4297959.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(46484..47371)
                     /gene="pdxS"
                     /locus_tag="O2692_000310"
     CDS             complement(46484..47371)
                     /gene="pdxS"
                     /locus_tag="O2692_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HCZ4297960.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            47475..48857
                     /locus_tag="O2692_000311"
     CDS             47475..48857
                     /locus_tag="O2692_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCZ4297961.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKSLIIK"
BASE COUNT        14827 a         9397 c         7104 g        17540 t
ORIGIN      
        1 aatctaagct tgttttcaat tcgctattac atcttatttt aattaataac attgctcaca
       61 acagtaatat caaaggctta ctattggtgc attagaacaa aaaataggca tgtgacttaa
      121 aagcattctc aatgaaatac cttaagctac atacctattt ttaatgtata tttacatatt
      181 ttttaatatt attttaaatg ttcatatgga ctattattca caaaagatac aatttgatcg
      241 acaaataatc ttgctcgtaa ttggaattca ctatcattta agtaatatga accatcttca
      301 tttttagcaa aatcagaatc atgtgttgct atttgagttg gtacagcaat gcctagtaaa
      361 cttctgacga ttactcttaa atgtgacaat ggctctgaac taacaatacc accactattt
      421 cctattaagc ctacaggttt cattttaaaa taatccatat ttagatgatc taatgcattt
      481 ttcaatattc cagaatatga accatgatag tttggcgttc ctaatattaa aaagtccgcc
      541 gccattgctt tctcttttaa atctttcata ttttgtttga tttcatcaat agacggtgtt
      601 gttcctgaaa aatctaattg atttaacggt ttttctgcta aatcgaatat ttccgcttca
      661 atatcatgtg ttttaaaatg ctcagttaag tatcttgcta gtgcacttgt atgtgaattc
      721 acttgtgcac tgccaataat aattaatcct ttcatatttg acccctcttt ttttattaaa
      781 cttgttgttc acgtaataat ttcataatag cttttccaac accactattt tcattcgtat
      841 ctgtgacata tttcgctatt tttttaactt cttctgcacc attttccatc gcaactggat
      901 agccaacttt ctctaacatt gataagtcat ttaaattgtc acctattgac atgacttctt
      961 tcatttcaat ccctaatctt tcggcaattg tttctagcgc aatacctttt tgtgcatctg
     1021 aatgcgttat ttctatattt cctctcgaag atgatgatat agctaaattc ggagattcag
     1081 ctaaaatttt actagctttg tcaatttttt ctaaatttcc atcaaatgct aatattttca
     1141 taattaattc accaggtatg ttttcaatag catcataatt atcaacaact ttcaacgtac
     1201 cattatctat gcgtctttga ataccatttt taatacgctc aacgtttgca tgttgacctg
     1261 cacgctcagc aatatctatg taaatgtcta aatctctttg tggatcttca gtataaatcg
     1321 cacgactcgt gtatacttga taataaatac ctgcatcttt taaaacattt gtaattttgt
     1381 gtactaacga tttattaagg tgtgaagtgc tcattacatt gaaagtttca tcacgtactt
     1441 cagcaccatt caaacaaata tatggtactg ttaaatctgt gtcagcaact ggtgcttgtg
     1501 cttcataaaa tgctcgacct gtcgcgataa caaccgttat cccttgttct tgagcgtatt
     1561 taatcgcatc aatattaggt tgagaaattt catgtgctgc attaagtagc gtgccatcca
     1621 tatcagtggc tattagtttt atcattatgt tacctcgttt cgtaaattta aaatcttgtt
     1681 cttaaataag aatatatact cagcgcacat acttttctat taacatttat attgtcatta
     1741 atttatcata taatgtaatt ctaacaaatt ttaattagta tgtactatcg tctaattggt
     1801 ggatttctta ttggctctta agtttttaaa aaatgttgtt aataatgtgc tacatgcttc
     1861 tttaagtaca cctttatcaa caattgcacg atgattaaaa ttagattgtt gcaataaatt
     1921 cattaaactg ccactacaac cacctttagg atcatctgcg ccatagacga ctcttggaat
     1981 gcgactcatt acaattgttc ctgcgcacat gacacatggt tctaaggtta catataatgt
     2041 gcaaccttct aaacgccaac tacctaacac tttggctgca cgttcaattg caatatgttc
     2101 agcatgcgcc gttggttgtt gtagtgtttc tcttaaatta tgtgctctag cgataacttc
     2161 atcatcttta gtgatgatag cacctatagg tacttcgcct agttgagctg cttttttagc
     2221 ttcttcaatc gctaatgtca taaaatatat atcatttgtc atttatgtcc agatacctca
     2281 cttatggtac aatactcaat gtctatttta acatttggag atgtgaatat gaacaaacct
     2341 tttatagcaa ttgaaggtcc tattggcgta ggtaaatctt cacttgcaca caaattaagt
     2401 caaactttag atttttatga agaaaaagaa atcatcacag aaaatccatt tttatcagac
     2461 ttttatgaag atatctctaa atggagtttt caaactgaaa tgttcttttt atgcaataga
     2521 tataagcaat ttcaagatgt aacacaacta aatcaaggtg tagttagtga ttatcatata
     2581 cataaaaata agatatttgc taaaaatact ttgagttctg ttgaatttca gaaattcagt
     2641 aaaatttatg atattttaac tgaagatatg attatgccga atatgattat ctttttagat
     2701 gcagaccttg atgtgttaaa atctagaatt gctaaacgta accgtagttt tgagcatcaa
     2761 atagaagatg aatatctgtt aaagttaaaa aaagattatc gtgagtatta tgagtcctta
     2821 caaagtaatg gttcaaatgt agttttaatc gatacaactt ctattgattt tcttaaaaat
     2881 gaacaagatt acgaagatat attacatatt atattaccta tgataggaga tattaccaat
     2941 gaataattac ggtattccac aaaatgccat tataaccatt gcaggtacag ttggtgttgg
     3001 aaaatcaaca ctaacgcaag cacttgcaga taaattaaac tttaaaacgt cttttgaaaa
     3061 tgtcgaacat aatccatatt tagataaatt ttacagcgat tttgaacgat ggagtttcca
     3121 tttgcaaatt tacttcttag ctgaacgttt taaagaacaa aagcgtatgt ttgaatatgg
     3181 tggtggcttt gtccaagatc gatcaattta tgaagatgtt gatatttttg caaaaatgca
     3241 tgaagaagaa ggcacaatga gtaaagaaga tttcaaaaca tattcagact tatttaatgc
     3301 catggtcatg acaccttatt ttcctaaacc tgatgtaatg atttatttag aatgtaacta
     3361 tgatgaggtc attgatcgta ttattgaacg tggtcgcgaa atggaaatta atacagaccc
     3421 tgaatactgg aaaaagctat ttaaacgcta tgacgattgg attaatagct ttaatgcatg
     3481 tccagttgta cgtatcaata ttaatgaata tgatatccat aaggaccccg aatctttaaa
     3541 tcctatgata aacaaaattg ctcgaattat tcaaacatat cgacaagtag atacacgata
     3601 aaagactaaa gacatagcgt atatgtttat attcaatgta tattccatag atattatcga
     3661 ttattttatc aattctatcg aatacattaa ttcacatata cactatgtct ttctttttaa
     3721 tttaaagctt ctaaaatatc tgccgcacta tttaaaataa tatcagcttc atgtaattct
     3781 tcttttgttg caatacctgt taatacacct attgccatac ctaaatttgc attacttgct
     3841 gtcttcatat cattagcagt gtctcctact atagctactt tctgaggatc tacattatat
     3901 tgctcaaata aaggcgataa tactttagga tttggcttct cataggcatc cgcttcggta
     3961 gaaatgatca aatcgaacaa cgaggtagca ttggtatgtg ctaaaaattg ttctacacct
     4021 tttttagtat cactcgtaac aataccaagt tgatagcctt ttgctttcaa atcgataagt
     4081 gcttctttaa caccttctac ccaattaatt tcaggaatac gttcatctac cagcttttga
     4141 cttgttgact tggaccagtc ggttgtatct tgtcccgtca catcattaaa tgcctggata
     4201 atttgttgta aagatcctga acccatcact gattttggat caatagattc tttaatgaca
     4261 ccgagttgtc ttaaagcagc ttctttatta tgtactggga aagtctcaag caatgattgt
     4321 acaaatcgta cccctatttt ttcccaactt ctatcaaatt caattaacgt accatcttta
     4381 tcaaataata tccattccat tgatatcaat actcctattt atttatttcg tattatgctg
     4441 attctatgat attcgttatc ccctgaaaat gaactcgtag tattgttcta tttaaatatt
     4501 gaattaaata taataataag tgaaatcccc ttcaatactt aacaataaac attgtaaact
     4561 taatttatta ccatgcttcg cttcattgaa agggatttta gtcatgatta acttttgcat
     4621 attgttttca tgattatatt caatttttat taatattttg gtacaacgac tctccaacca
     4681 tttttatctt ctaaagtacc attttgaata ccagtataga cgtcgtataa ttttcgagta
     4741 atttcaccag tctcattatt attaataacg atttcacgat cttcgtatct caatgtaccc
     4801 acaggtgaaa taactgctgc agtaccacta ccaaatactt ctgttaactc acctttatca
     4861 tatgattcga ataattcatc gattgaaacg cggcgctctt cgacttcata tcctaagttt
     4921 ttagctaatt cgataataga tttacgtgta ataccaggta aaatactgcc attcaactct
     4981 ggtgtaatta ctttgccatt ttcaacgaag aaaatgttca tgctaccaac ttcttcgata
     5041 tatttctgtt caacaccatc aagccataat acttggtcat aacctaattt atttgcatta
     5101 gtttgtgcta ataaacttgc cgcatagtta cctgcaactt ttgcaaagcc tacaccgcca
     5161 cgaacagcac gcacatattc atcttctaca tagattttag ttggttttaa agtttcacca
     5221 ccataatatg cacctgaagg agataaaata attaataatt tatactgatg tgatgcacca
     5281 acgccaagtg ccccttctgt tgcaaaaaca aatggacgaa tatataatga ttgaccttcc
     5341 ccttcaggaa tccaatctct ttcaatatca actaattgtt ttagcccctc taacaattct
     5401 gcttcgtcta cttgaggcat ttctaatcgt gctaacgagt tattaagacg cttaaaattt
     5461 tcttcaggac ggaaaagtgc aacttcccca tctcttttat atgcttttaa tccttcgaat
     5521 accgattgac cataatgaac accttgtgca gcaggtgaaa tttcaatagg accataaggt
     5581 actatcttca aatcatgcca tcctttatct gcatcataat cataactcaa catataatca
     5641 gtaaaatatt taccaaaacc tagttgagat gtatttggtt tttgttttaa tgtttctcgt
     5701 cgttcaactt taactgcttg tgacatggtg attgcctcct aataatattg tataagaatt
     5761 tgtttaactt aaattataac aatccatatt ttgctgttca acaaattttc taaaaattca
     5821 aaattaatta acagatttct agaaagacta tatcttttag tataaacgta ttaatttcac
     5881 agagacaagt aatctgtgtt ttactaatat actttacata caaaaaactc tttactttaa
     5941 aatgaactaa gctcgcgaat tcaataagta taatgaataa tattagaatt catgcactag
     6001 tttattaaaa taaagagtaa tttaaaatat cattccgtgt attaaagtga atggaaatga
     6061 ttagttatta tttttaacag tatctttttg ttcaatagct tctaacatta atttagtcat
     6121 gctcgctaaa tcatatttag gatcaaatcc ccattcgcca cgtgaacagc ttgtatcaat
     6181 agaatccggc caactattag cgataccttg tctaatagga tcaacatcgt aatctaatgt
     6241 aaaattggga tagtattctt gaattgcttc ttttaccatc tctggatcaa aactcattgc
     6301 gctcaaatta taaccatttc tagtttctaa tttagcgtcg tctgcttcca taagtttaat
     6361 aattgcttca attgcatcat ccatatacat catatccata tacgtgcctt tatctatgaa
     6421 gcttgtataa tgaccctctc ttactgcttt gaagtatatt tcaacagcat agtctgtagt
     6481 accgccacct ggctctttaa catgcgagat taaacctggg aatctaacac ttcttgtatc
     6541 tacaccaaaa cgtttgaaat agtattgaca caataattct ccagctactt tatttacacc
     6601 atacattgtc gtaggttgct gaatcgttac ttgtggcgta ttaactttag gagttgagtc
     6661 tccaaatgca ccaattgaac ttggtgtgaa aaagtgcaaa ttataagttc ttgcagcttc
     6721 taatgcattc attaatccac ccatatttaa atcccaagct agaattggat ttttctcagc
     6781 agttgctgat aataatgctg ccatatgcat tagactatcc gcttcaaagt ccctaactaa
     6841 ctcaaacata cggtcacgat ctgttacgtc taagatttca aatggtccat tttgtacagg
     6901 tgagtctgct tcaggttccc taatatctgt agcaagaaca ttatctgtcc cataaatttc
     6961 tctgcactta acaactaatt ctgtaccaat ttgtcctaat gcaccagtaa tcataatttt
     7021 tttcatagaa atatctcctt tgtctcttct atatagctat agtccatcac aagcggacat
     7081 aatattcatt ttcataataa ttataatata aaagcgcttt cttgtatata tgacatgtac
     7141 atgttgctga tatttctgta aatggaaatt ctagttgtat taattgattt tagtaattta
     7201 tagcgtttat tattgctaat tactgtcaaa ttaaattttt tatccctcaa ctcttaaact
     7261 ctggatatct ttcattatat tagctttttt ataaccatgg atatcatgta aagccttata
     7321 agctttaaat aatgtttcat acctttgtac tttttccgct tctggattaa tttcgtataa
     7381 aattggctct ttcatagcat caactgcgtc attaattgaa ttatgtgcgc caccactgac
     7441 tgcacctaat attgctgcgc ctattgctgg tgcatactca ctatccataa caattagttt
     7501 tttattcagt acattcgcat agatatccat cataacagca ttctttttag gtataccgcc
     7561 acatgcaaat accttttcta cttccatatg ccaatcttga tactgttgca taatcatctt
     7621 agtaccaaat gctgtagctt ctaaatatgc tctataaata tcctcatgct tagtttgtaa
     7681 agttaatcca aagatacaac ctgttaaatt gctatcactt aatacacttc gatttccatt
     7741 atgccaatca agagcaatga gcccactttc acctggcatt tgatgtttta tcttttcatt
     7801 cattaattca aatacagtca tatttctatt ttctgcttca tctacatatg actttggtgc
     7861 ttgcttagcg acatactcaa acaaatcacc tactgctgat tgccccgctt cataagcaaa
     7921 taattctgga ataattgctc cttttacaga acctgatata cctggcactt gatgttgctt
     7981 ttcatttaac ataagatggc atgtgcttgt tcccatcacc atagtcattt ctttatcttt
     8041 ttcagaccca atacctaata aactagcatg ggcatcaata ataaaaggac ttaccatagt
     8101 ttcttttgat aatcctaatt tctgtgccat tttatcatcc agtttcccta ctgcttcacc
     8161 aatattaaca accggtgcag atactttatc ttgaattact tttgataatt tggggtctat
     8221 tttatcaaat aaatcataat gaaaccctgt ttcttcttcc caaaatgctt tgaaacctaa
     8281 tccacaattc gagcgtacat ttttattagt taatttattt acaatccaat cgcccgcttc
     8341 cataatataa gccgtttttt ccataatttc tggtgctcga ttcatgacct ccattatttt
     8401 gggaatcatc cattcactac taacattata tccataatgg cctaaccact tattattatt
     8461 ttcaatagca gtttgatata atttctctgc ctctttatat gcaccatgat gtttccaaag
     8521 tttcacatac gcatgtggat tgtttttaaa ttgttttaag ttatgtaccg ggttaaggtt
     8581 ttcgtcagta aaaataatag tagatgaagt aaagtctata ccaataccta ctatattgtc
     8641 tggatctatt tttgattcac gtactatata tgatattcct tcttccataa tttctaaata
     8701 atcattacta ttttgaagtg catatgtatg tggtattttc aaaccattta attcactctc
     8761 aatgacacca tgtgtatatg gtttcacaaa ttttgatact acttgaccgt tagttgtatt
     8821 aattaaaaac acacggcctg aagcagttcc ataatcaatt ccaatgctat aagacatcat
     8881 gtttcctcca tatcgctagt tgcggttatt agttttgcaa gcgttttctt aaatcatact
     8941 cataataggt tctattttac aagtatcact taaattttgc caaaaaaaaa gagccctcat
     9001 acatttacat atgaggctcc tctaattaat atttaattat ttattttgta ttgcatttaa
     9061 catttcatta actgctaatt ttcctaactc atttgaagct aaaggactgt cacctgttaa
     9121 taatttacga tcttttaacg ttcttcctgt catatcgtcg ttaactactt ttaatccttg
     9181 tttagttaat aaatcagcaa ctaaccattt caagcgtcca ggtaaataac ctatttcaat
     9241 atttgcacct tcatctaatg agtcagggaa gacacaaaca gagtatcctt ctaatggaga
     9301 tttttctctg ttaagccctg ctgaaagtag tgctgctggt ccatgacata atgtaactat
     9361 aaagcggtca ttgtctaatg cccaatctaa tgtttgttga acgtcctcac tttcagaaat
     9421 accaacaact gcagcatgtc cgcctgggat aaagacagat aaatagtctg aatcaggtcc
     9481 taattcattt ttaatcacat ctgctaattt ttttggctgt tttaattttt ctttcaattt
     9541 attataagta cttataactg cctcgtcttc agttggcata gcccataatt ctaatttaac
     9601 tggataacca gataatgtcg caacatcaac gtcaaaacct gcttccatta aatgatgtaa
     9661 aggtaataac atttcaacag gatgattacc cgtagagaac atttttccat tttccaataa
     9721 tacatatcgc tcttcagcag caatcatcaa tactttccat ttaccatctt tataggcacc
     9781 tttgtgttca acaccatcaa aatctgtttt aggtgctgta tattgactaa gggaatatgg
     9841 tgatgggaaa aatgcgttat cttctgcttt atctggcgtt ggttgcttac ttaattcatt
     9901 tacatcttgt gacatagttt attcctactt tctctatata gttactacta gtatagacat
     9961 atactacaca ctataaacat gtattactat taaaatgctc attatcaaaa ataatcaaaa
    10021 tgaatttctt tgaaaaaaat acaactaaat tctacaagat gcttttaaaa ttcaaacttc
    10081 aattttgggg agcactaaac attgatatac gaacttactg ttaataaact aaacattaaa
    10141 aaaagcccac gacaatattt gccgtgggaa taaataaata ttaaatcaac ttcatttctt
    10201 ttcctacttt ttcataagcc gcaattgctt catctaacat gtcttttgta tgtgcagctg
    10261 taggcatatt tcttacacgt cctgtacctc ttggtactgt tgggaaaacg atagatttca
    10321 catagacacc ttcgtctttt aaacgcttac taaattcttg agttgttttt tcatcaccaa
    10381 taattactgg tgtaattgga gtttctgact cacctgtatc atatcctaat tttgacaatc
    10441 cattttttaa atattgtgca ttgttccata atttatcatg taattcagtt gaatccatta
    10501 actttttaac tgcttcagtt attgctttgg tatccccagg tgctaatgat gtagagaata
    10561 agaatggtcg tgattgtgct tttaaccaat ctattaactc ttttgtacct gctacataac
    10621 cgccaacgac accaattgct ttagaaagcg tacctatttg gaaatcgatt ttatcttgta
    10681 aaccaaaatg tttaaccgta ccagcgcctt tacccataac acctgaacca tgagcgtcgt
    10741 caacataagt taataaacca aattcttctg caatttctac aatttcaggt aatttagcca
    10801 catcaccatc catactaaaa acgccatcag tgatatacat cactttattg tattgacctg
    10861 attcaactgc ttcttttgct ttcgcacgta aatcatccat gtctgaatgg ttaactcgaa
    10921 taattttagc tttagataag cgacatccat caataattga tgcatgatta agctcatctg
    10981 ataaaatagc atcattttta ttcatgacag ctgaaatagc agccatatta caattaaatc
    11041 ctgattgata agctattgca gcttctgttc ctttaaattt tgctagtgtt tcttctaatt
    11101 cgtcgtgtaa atctaatgta ccattgattg tacgaacagc gcctgcacct acaccatgtg
    11161 tatcaatagc tgcttttgca gctgatttca aatcttcatt tgttgctagt cctaaataat
    11221 tatttgaaga taagttaatg tatgatttcc cattgatttt gatttctggt ccgtttgcac
    11281 cttcaattgt atctatttca ttatacaaac cattttcttt tagataattt atattttcct
    11341 ctaaaaactc atgtaatgat tgaaccacag tatataatcc cccttaagta ttgtttggta
    11401 atacctttat catacagtaa ttctacataa cttgaaagcc ttatcattca tagataaaat
    11461 atctcaaagc aatcatttag tacgataatt tcataaaatg ttatactata aacattattt
    11521 tagaaagtag tgataagggt gttagattgg ttccaactag caaataataa agaaaataaa
    11581 acaattcaac tcagacgtta tttacaccaa tatcctgagt tatcttttga agaatttcaa
    11641 acacatgatt atattgttaa ccaattaagc cagttatctt gtgatattga aactcctatc
    11701 ggccgcaatg gtatcaaagc cacatttaaa ggcttgggca caggacctac cattgcatta
    11761 agagctgact ttgatgcgtt acctgtcgag gaattaaacg atgtgcctta taagtctaaa
    11821 aatccaggat gtatgcatgc gtgtgggcat gatggtcata cagcaatttt acttactgta
    11881 gcagagattt tagatgaaca taaacattta ttagaaggta atgttgtatt aatattccag
    11941 tacggtgaag aaatcatgcc aggtggttct caagaaatga tagatgctgg atgtctggaa
    12001 aatgtagata gaatttacgg tacacattta tggagtggtt atcctactgg aactattcat
    12061 tcacgtgcgg gggcaatcat ggcctctcca gatgaattta gcgtcacatt taaaggtcgt
    12121 ggtggtcatg gtgccaaacc tcatgaaacc attgacccaa tagtaatcat ggcagagttc
    12181 attttaagtg cacaaaaaat aatttcacgt acgatcgatc cagttaaaca agctgtactt
    12241 tcatttggaa tgattcaagc aggcaccaca gatagcgtta ttcctgatca agcattttgt
    12301 aaaggtacag ttagaacttt cgattcagat atacagaatc atgtaatgga caaaatggat
    12361 aagcttctac aaggtttagc aatagcaaat gatattaact atgatttgaa ttatattaaa
    12421 gggtatttgc ctgtccataa taacgagaaa gcatatcaag ttattaaaga agcaacaaat
    12481 gacttacatg tacgctttaa tgaatcagat ttaatgatga ttggagaaga tttttcacat
    12541 tatttgaaag tacgaccagg tgcattcttt ctgactggtt gtggtaatga aagtaaaggt
    12601 attacagcgc cacaccataa tcccaaattt gatattgatg aaaaatcatt aaaatacgct
    12661 gtagctgtat ttttaaaaat catagaatta gagcaagtat ttaaaacaaa ttaagcattt
    12721 aaattaattg aaagatatga atctgagaca taaactaatg aaatgctccc ttcaaagtag
    12781 acattgaaaa aatgaaaact ttgaagggag cattttacaa tgtccaagac tcaacaattt
    12841 tatttaaaca gtattatcag ttataagttt tattagtcag tctttcatca attattgttc
    12901 ttctaaatcg aatagccatt acaaaattta cacattaaaa aaagacccct cgattgaggg
    12961 atcttctttt tatataaaat ctaagaaatt agaaattatt taatgatttc agtaacaacg
    13021 cctgatccta cagtacgtcc accttcacgg attgagaaac gagtaccgtc ttcaatcgcg
    13081 attggagcga ttaattctac tgtcatttca acgttatcac caggcattac catttcagta
    13141 ccttctggta agtgaacaac accagttacg tcagtagtac ggaaatagaa ttgtggacga
    13201 tagtttgaga agaatggagt gtgacgtcca ccttcgtctt ttgataatac gtatacttct
    13261 gctttgaatt cagtatgtgg tgtaattgaa ccaggagcag ctaatacttg accacgttgt
    13321 acgtcttcac gagcaacacc acgtaataat gcaccaatgt tgtcaccagc ttcagcgtag
    13381 tctaataatt tacggaacat ttcaacacct gtaacagttg ttttagatgt gtcatgtaaa
    13441 ccgatgattt caacttcttc accaactttg atttgaccac gttcaacacg gcctgtagca
    13501 acagtaccac gaccagtgat tgagaatacg tcctcaactg gcatcatgaa tggtttgtca
    13561 gaatcacgtt ctggagttgg aatgtaagta tctacagctt ccattaattc taagattttt
    13621 tcttcgtatt gagcatcgcc ttctaaagct tttaatgctg aaccagcgat tacaggtaca
    13681 tcgtcacctg ggaagtcata ttcgcttaat aagtcacgaa cttccatttc tactaattct
    13741 aataattctt catcgtcaac catgtcaact ttgtttaaga atactactaa tgctggtaca
    13801 ccaacgttac gtgataaaag aatgtgttca cgagtttgtg gcattggacc gtcagcagca
    13861 gatactacta agataccgcc gtccatttga gcagcaccag tgatcatgtt tttaacgtag
    13921 tcagcgtgtc ctgggcagtc aacgtgagcg tagtgacgtt tgtcagtttg gtactcaatg
    13981 tgagaagtat tgattgtgat accacgttct ttttcttctg gagcgttgtc aatcatgtca
    14041 tatgattgtg caactgagtc accatttttt gctaatacag tagcgattgc tgctgttaat
    14101 gttgttttac catggtcaac gtgaccgata gtaccgatat tggcatgttc tttagaacga
    14161 tcgaattttt cttttgccat tataaaatct ctcctattct aattaaaaat aatatttaaa
    14221 tttatctctc atgatagttt ctcaccatca tgagaaatta tttatagcga tgcaaaaaga
    14281 attttacaat tcttttataa tgcattcacc ccaaaaaatc aaactataaa gatgatagct
    14341 tacatttaag ctcaccttgt attttaacct aggctagcta gtcaaaacaa gttatattat
    14401 tcacctttat ttttcttgat aatatcttca gcgattgatt ttggaacttc agcatagtga
    14461 tcgaagtaca tagtgtaagt accgcgacct tgagtgtttg aacgtaatga tgttgcataa
    14521 ccgaacattt ctgaaagtgg tacataagca ttaacaactt gtgcattacc acgaggttcc
    14581 ataccatcaa cacgtccacg acgagatgtt acgtcaccca tgatatcacc catgtactct
    14641 tcaggcattt caatagttac tttcatcatt ggttctaaga ttacaggatc acatttttta
    14701 gcagcttctt taagtgctaa tgatgcagca attttgaagg ccatttcaga tgaatcgaca
    14761 tcatggtatg aaccatcata taatttagct ttaacatcaa ttaaaggata acctgctaaa
    14821 acaccatttt ccatagcatc tttaagacca gcttctactg atggaatgta ttcacgagga
    14881 actacaccac caacgatagc gttttcgaat tcgaaacctg cgcctgtttc gtttggtgtg
    14941 aattcaatgt gaacatcacc gtattgacca cgaccaccag attgacgaga gaatttacct
    15001 tgaacttgtg cagatgattt gaatgtttca cgatatgaaa ccattggagc acctacgtta
    15061 cattcaacgt tgaattcttt cttcatacgg tctactaaga tgtctaagtg aagctcaccc
    15121 ataccaccga tgataacttg tccagtttct tcgtcagtgt gtgcatggaa tgttgggtct
    15181 tcttcttgta atttaactaa agcttgagtc attttatctt ggtcagcttt agattttggc
    15241 tctactgata agtgaataac tggctctggg aattccattg attccaagat aatgtcattt
    15301 ttctcaccac ataaagtatc accagtacct gtatctttaa gacctaccgc agcagcgata
    15361 tctccagagt atacagtatc gatttcttga cgtgagttag cgtgcatttg taataaacga
    15421 cctacacgtt cacgtttacc tttagtagag ttcttaacgt atgaaccaga tgtcattgta
    15481 cctgaataca cacggaagaa tgttaattta ccaacataag ggtcagtcat aactttgaac
    15541 gctaatgcag cgaattcagc tgaatcgtct gctttcgcga ttacttcttc ttcagggttg
    15601 ctagcacggt gaccaataat tggtttaacg tctagtggtg aaggtaagta atcaattaca
    15661 gcgtcaagca ttaattgaac acctttgttt ttgaaagctg taccacaaag tactgggtag
    15721 aattctacgt tagtagtagc ttggcggata gcttctttta attcagaaac tgaaatttct
    15781 tcgtcaccaa gatatttttc cattaattcg tcgctagttt ctgcaactgc ttcgattaag
    15841 ctagcacgag cttcttcagc tctatctaag tggtcttcgg gaatttcaat ttcttcaatt
    15901 tcagtaccta aatcatttgt atatttgaaa catttcattt caactaagtc aatgattgct
    15961 tcgaattcgt cttccgcacc aattggtaat tggattggag cagcgttagc ttgtaaacga
    16021 tcatgtaatg tacttacaga gtattcgaag ttagcaccta atttgtccat tttgtttaca
    16081 aatacgatac gtggaacacc ataagttgta gcctgacgcc aaactgtttc agtttgaggt
    16141 tcaacacctg attgtgcatc aagtactgta actgctccgt caagtacacg taatgaacgt
    16201 tcaacttcta cagtgaagtc tacgtgtcca ggtgtatcga taatgtttac acggtgacct
    16261 tcccaagctg ctgttgttgc agcagatgtg atagtaatac cacggtcttg ttcttgctcc
    16321 atccagtcca tttgtgaagc accttcgtgt gtttcaccaa ttttgtggat acggccagtg
    16381 taataaagaa tacgttcagt cgtagtcgtt ttaccagcat caatgtgagc catgataccg
    16441 atattacgag ttttttctaa tgaaaattct ctagccatgt attttttctc cttccagttt
    16501 atattactga ataaatacga tagatatggc gatgccctaa gcatgaacgc ttaatgaaaa
    16561 ttcattaata tagaacacac tcagggtaaa agcttttatc ttaccaacgg tagtgagcaa
    16621 atgctttgtt tgcttcagcc attttgtgag tgtcctcacg tttcttaacg gcaccacctg
    16681 tattatttgc tgcatctaaa atttcgttag ctaaacgatc ttccatcgtt ttttcaccac
    16741 gaagacgtgc atagttaact aaccaacgta aacctaaagt agtacgacgc tctggacgaa
    16801 cttctactgg tacttgatag ttagaaccac ctacgcgacg agctttaact tctaatactg
    16861 gcataatgtt gttgattgct tcttcgaata cttctaatgc atcacgacca ctgcgttgtt
    16921 caactaggtc gaatgctgaa taaagaattc tttgtgctgt tccacgttta ccatctaaca
    16981 taattttgtt aattaattta gttactaact tagagttatg aattggatct ggtaatacgt
    17041 ctcttttagg tactgatcct ttacgaggca taatgtaaat cctcccttcc ttatattaaa
    17101 tattttaagt ttaagattta attaaaaact aaattcttag tttttaggtt tcttagttcc
    17161 gtataatgaa cgaccttgtc tacgtccgtc aacacctgaa gtatcaagtg ctccacgtac
    17221 aatatggtaa cgcacacctg gtaagtcttt tacacgtcca ccacgtacaa gtacaacact
    17281 gtgttcttgt aagttatgtc cgataccagg gatgtatgcg ttaatttcga tgttgtttga
    17341 taaacgcaca cgtgcatatt tacgtaacgc tgagttaggt tttttaggtg tcattgtacc
    17401 tacacgagta catacaccac gtttttgtgg tgagtttaag tcagtaaatt ttttcttttt
    17461 actgttgaaa cctttattta aagctggaga atctgatttt ttgattttgc tttgtcttgg
    17521 tttacgtact aattggttaa tagttggcat gtgatatgtc ctcctctctt cactttttaa
    17581 tcccacacat ccaggtggtt catttttagg ttaaataaaa tttagtaagt aaaacactta
    17641 ctaattctca tttaatcaat gctactattg tcgcattgac gttaatacct acatgtttac
    17701 ccaaagcatg tttgcttttg aaaaaagata caggtatatt tttctgattg atttggctta
    17761 acacgcgagt cattaaatat acttcaacgt cttcagcaat aatcaaagat gtaacttgat
    17821 ctttctttaa cgctttaagc gtttctttaa gaccaactac aaaatgttgt ttgttaaagc
    17881 gtgcaacttt ttccttagac aataatttat cctccaaagt tctgcttgca tcaaccttta
    17941 atatattaac attgaacaag agaattcgtc aacattaaat caaataattt acataaaaat
    18001 aacaagaggc taaagcatta gcctctgtta tatacttgtt attccgttac ttcagtttga
    18061 gattcaactt ctgcaactgg tttagctgtt ttttcgtatt ttacgtcgct ataacgtctc
    18121 ataccagtac cagctggaat taacttacca ataattacgt tttctttaag acctaataag
    18181 tcatcacgct tacctttaat tgctgcatct gtaagaactc ttgttgtttc ttggaatgat
    18241 gctgcagata agaaactttc tgtttcaagt gatgctttag taataccaag taatactggt
    18301 ttagctgttg caggacgctt acggtgttta aatgcttctc tatttgcatc tgtaaagtta
    18361 tgaatatcaa ctaatgaacc tggtaataac ttcgtatcac ctgcttcgat aattctaact
    18421 ttacgtaaca tttgtcgaac cataacctca acgtgtttat cgtcgatttc tacaccttgc
    18481 atacggtaaa ctttttgtac ttcttttaat aagtagcttt cagtcgcgtt taatccagca
    18541 acagataagt aattcttagg ttcaatagaa ccttcagtta atacttcacc acgttgaact
    18601 ggttgaccga tttctacaat aattcttgaa gtacctgaag caaggtatga tcttgtttca
    18661 ttagcacctt taacaacaat ttcttgttgt ctatcttttg ctaatttaat atcttctacg
    18721 acaccttcga tttccgtaat taccgcttga cctttagggt tacgtgcttc gaaaatctct
    18781 tgaatacgag gaagaccttg tgtgatatcg ctacctgcta ccccacctgt atggaatgta
    18841 cgcattgtaa gctgtgtacc tggttcaccg atagattggg ctgcaattgt accaactgct
    18901 tcaccaactt caactttttc accagtagca aggtttttac cgtaacattt ttcacaaaca
    18961 ccatgtcgtg cgttacaagt aaatgctgag cgaatataca tttgttcaat accagcatct
    19021 gtaattttct tagcaatttc aggtgtaatt aattcatcag gacgaatgat tatttcatca
    19081 gtttcaggat gatgaattgt ttctttagaa taacgacctt caatacgttc gataaatggt
    19141 tcaatcattt ctgtaccttc tttaatatca gaaactaata aacctctatc agtaccacag
    19201 tcttcttcac gaacaataac atcttgtgcc acgtcaacaa gacgacgagt aagatatcct
    19261 gagtcagctg ttttaagtgc tgtatcggca agacctttac gtgcaccgtg agttgagatg
    19321 aagtattcaa gtactgttaa accttcacgg aatgaagatg tgattggtaa ttcgataatc
    19381 ttaccagatg gtgcggccat caatccacgc atacctgcta actgtgtaaa gttagatgcg
    19441 ttaccacggg cacctgaatc actcatcatg aagattgggt tagttttatc aagtgattgc
    19501 atcaattcac cttgaatttg atcttttgca tctgtccaaa tttcaacaac tgcattatat
    19561 ctttcttctt cagtgattaa accacggttg aattgttttg taattctgtc gactaatttt
    19621 tcatgctcat caagtatttg ttgcttatca ggtaatacta cgatatcagc aacacctact
    19681 gtaataccag ctttagatga gaatttgaat cctaagtctt tcatacggtc taacatcatt
    19741 gatgtatcag tgatgctaaa tctgttgaat acttctgcaa taatattacc taagaatttt
    19801 ttgttgaaag gttcaattaa ttcttcattt tcaaagtatt cttttaatcc accttcacct
    19861 aaagttgtag gatcgatgaa atatctgttt ggtgtctttc tttctaagtt ttcttgcgta
    19921 ggttcattaa tataagcaaa tgaatctgga atgatttcat tgaatataat tttacctact
    19981 gacgtagcaa gaatcttttt gttttgttct tcagtaaatg ttggattatt gaacgaactt
    20041 gcatgtacac caattctagt gtgtaaatgt acaaagccat ttgcatatgc ttttaatact
    20101 tcatttgtat tattaaagat tgcgcctgta tttactgcat cttttctttc taaagtaagg
    20161 taatagttac caagtaccat atcttgtgat ggtgtaacta caggtttacc atctttaggg
    20221 ttcaagatgt tttgtgctgc taacatcaac attcttgctt cagcttgtgc ctcttttgat
    20281 aaaggaacgt gaaccgccat ttggtcaccg tcaaagtcag cgttataagc tgttgttaca
    20341 agtggatgta gacgaatcgc acgaccttca actaaagttg gttcaaatgc ttgaatacca
    20401 agtctatgaa gtgttggtgc acggttaagt aatacaggat gttctctaat tacttcttcc
    20461 aatacgtccc aaacttcatc atccatacgt tcgattttac tcttcgcatt tttaatgtta
    20521 gttgcaattt cacgttgaac taattctttc attacgaatg gtttaaatag ttcaagtgcc
    20581 atttcttttg gtaaaccaca ttggtacatt ttcaagcttg gacctactgc aataactgaa
    20641 cgtcctgaat agtcaacacg tttaccaagt aagttttgac ggaaacgacc ttgtttacct
    20701 tttaacatat gagataaaga ttttaatgga cggttacctg ggccagtaac tggacgacca
    20761 cgacgaccat tatcaattaa agcgtcaacg gcttcttgta acatacgttt ttcgttttga
    20821 acgatgatac caggtgcacc taaatctaat aaacgtttca aacgattatt tcgattaatt
    20881 acacgacggt ataagtcgtt taagtcactt gttgcaaatc gtccaccatc taattgaacc
    20941 attggacgaa tttctggtgg gatgattgga agtacatcta aaatcatcca tgaaggtttg
    21001 ttacctgaat tacggaatga ttcaacaact tctaaacgtt taattgcacg agtaagtctt
    21061 tgaccagtag ctgattccaa ctcatcgcgt aacaatttaa gttcttcgtc aagatcaatc
    21121 tcttcaagta aatctttaat accttctgca cccatttttg caacgaattg acctgggtat
    21181 ttatcataat aatctctgaa ttcagcttca gataataaag ttttcttttc taaaccagtt
    21241 ggacctggat ctacaacaac ataagaagca aagtaaataa cttcttctaa tgctcttggt
    21301 gacatgtcaa gtaataatcc catacgactt ggtatacctt tgaaatacca aatgtgagaa
    21361 actggagcag caagttcaat gtgacccatt ctttcacgac gtactttaga tttagttact
    21421 tcaactccac atctgtcaca gaccatgcct ttgtagcgaa cacgtttgta tttaccacaa
    21481 ctacattccc agtcttttgt aggtccgaaa attctttcac agaatagacc atctttttca
    21541 ggttttaatg tacggtagtt gattgtttca ggttttttaa cttcaccaaa agaccaagaa
    21601 cggatttttt caggtgaagc caatcctatt ttcatataat ggaaattatt tacatcaatc
    21661 aaggagccta cctccttcaa tttagattag ctgtgctatt tgattgattt gcatttattt
    21721 aaaatggctt aaagtactaa actgaatcat caataaaatt cagcttagta tctttttgcc
    21781 tgttttgtaa attgcgtatt aatcagtaac ttctttttgt gtttcaggag catcattttg
    21841 ttgtaaatct actttgcgtt ctacaacatc atcgtcatca acgtctgtca tttcgatttc
    21901 attatcttgc tcatccataa cttttacatc taaacctaaa ctttgtaatt ctttcatcaa
    21961 tactcggaat gattctggaa cacttggtct agagatgttt tcacctttaa caatagcctc
    22021 gtatgttttc acacgtccta ctgtatcatc ggatttgtaa gttaagattt cttgtaatgt
    22081 gtatgcagca ccatatgctt caagtgccca tacctccatc tcaccaaaac gttgtccacc
    22141 gaattgcgct ttaccgccaa gtggttgttg tgtaacaagt gaatatggtc ctgttgaacg
    22201 cgcatgtaat ttatcatcaa ccatgtgcgc aagtttcaac atgtacatta cacctactga
    22261 aatacggtta tcgaatggtt cacctgtacg tccatcataa agtacagttt taccatcacg
    22321 agccatacca gcttcttcaa ttgttgacca tacatcgtca tcgtttgcac cgtcaaatac
    22381 tggtgatgca acgtgaatac caagattttt agcagccata cctaagtgta gctctaatac
    22441 ttgtccgatg ttcatacgag atggtacacc aagaggattt aacatgatat cgatcggacg
    22501 tccatctggt aagtaaggca tatcttcttc aggaacaatc ttagaaatga cacctttgtt
    22561 accatgtcga ccacacatct tatcaccaac atgaatttta cgtttttgaa cgatatatac
    22621 acgtactaat tggtttacac caggtgataa tgtatcgtcg ccttcttcac gattgaatac
    22681 ttttacatca agaacgatac cgccagcgcc gtgaggtaca cgtaatgaag tatctctaac
    22741 ttcacgtgct ttttcaccaa agattgcatg taacaatctt tcttcggcag ttaactcagt
    22801 tacaccttta ggcgttactt taccaactaa aatatctcca tcttttactt ctgcaccaat
    22861 ataaacgata ccacgatcgt ctaagttctt aagtgcactt tcagaaacat taggaatatc
    22921 tcttgtgatt tcttcaggtc ctaacttagt atcacgtgct tctgattcat actcttcaat
    22981 atgaatagaa gtatacacgt catctttcac aagtctttca ctcatgataa cggcatcctc
    23041 atagttgtaa ccgtcccaag tcatgaaacc aactactacg tttctaccta atgccatttc
    23101 tcctaattcc atagatggtc catctgctaa aatctcgtta tactcaacaa catctccaac
    23161 tgcaacgatt ggacgttggt tgtaacatgt acctgagttt gaacgtttaa atttagctaa
    23221 tggatagcga tctaattcac cttcatgctc aacgccgttc tcttcaacta gacgacgaac
    23281 aagaatttca ttagattcaa catgttcaac acgacctctg tgcttagctg taatagccgc
    23341 accagaatca cgtgctgcaa cgtgttccat acctgtacca acaaatggtg cttctggatt
    23401 catcaaaggc actgcttgac gttgcatgtt cgcacccatc aatgcacggt ttgagtcatc
    23461 attttctaag aatggaatac atgctgtcgc tgctgaaaca acttgcttcg gcgatacatc
    23521 catataatcc attttttctt tagccataac tgtattgtta ccacggaaac gacatacaac
    23581 ttcatcatcc atgaaacgac cattttcatc taatttagag tttgcttgtg ctacaacata
    23641 gctatcttct tcgtcagctg ttaaatagtc aatttgatca gtgatagcat gtgtatctaa
    23701 atcaacttta cgatatggtg tttcaataaa gccgaattca tttacacgtg cataacttga
    23761 taatgagtta atcaatccaa tgtttggtcc ctcaggtgtt tcaattggac acatacggcc
    23821 atagtgagag tagtgaacgt cacgtacttc catttgagca cgttcacgtg ttaaaccacc
    23881 aggtcctaat gctgatagac gacgtttatg cgttaactca gctaatgggt ttgcttggtc
    23941 catgaattgt gataattgag agctaccaaa gaattcttta atagatgcaa taacaggtcg
    24001 aatattaatt aattgttgag gtgtgataga ctcagtatct tgaattgaca ttctttcacg
    24061 tacaactctt tccattcttg ataaaccgat acggaattgg ttttgtagta attcacctac
    24121 agaacgtaaa cgacggttac ctaaatggtc aatatcatct gtatatccaa taccgcttaa
    24181 taagttaaag aagtaactca ttgaagcaat gatatctgct ggtgtaatgc atttaacttc
    24241 tgagtcaggg aaagcattac caattacagt tgtcgtacga ccttcatcat cgttaggaac
    24301 atatacttta attgattgaa tttctactgg ctcgtctata acgctaccat gcaattcaaa
    24361 cacttcgctg tttgcatttg attcaagtac atccatgatt tcgtcgattt tacgacgatc
    24421 aagcactgta ccttcttcaa ctacaatttc accagtttca gtatttacaa ttggctcagc
    24481 taatttttga ttaaataaac gatgttttaa atgtaatttt ttgtttgttt tataacgacc
    24541 cacgcttgct aagtcatagc gttttggatc aaagaaacgt gaatacaata gacttttagc
    24601 attttcaaca gttggtggtt cacctggacg taaacgttca tagatttcta ataacgcttg
    24661 ttcagtgttt tcagtgccgt ctttctctaa agtattacgt aaatattcat tgtcacctaa
    24721 aaggtcaaca atttcttggt cgcttgagaa acctaatgca cgtaacaata ctgttaatgg
    24781 tagtttacgt gttctatcaa tacgtacgta tacaacatct ttagcatctg tttcatattc
    24841 taaccatgca ccacggtttg gaataattgt tgcatcatag ttttcacgac catttttgtc
    24901 gattttttca ttgaaataaa cggatggtga acgaactaat tgagatacga ttacacgttc
    24961 tgcaccattg ataacgaacg tacctgtatc agtcattaat gggaaatcac ccataaagac
    25021 ttcttgttct ttaacttctc ctgtttcttt aatgattaga cgcactttta cacgaagagg
    25081 tgcagcataa gtagcgtcac ggtttttaga ttcttctaaa tcatattttg gttctcctaa
    25141 acggtaatcc acaaactcta atgacaaatt accagtaaaa tcttcaattg gagaaatgtc
    25201 tctaaacatt tcgattaaac cttctcttag gaaccactcg taagatttag tttgaatttc
    25261 tattaagttt ggtaattcta atacttctga aattctcgcg tagtttctac gtttacgatg
    25321 tcttccatat tggacaactt gacctgccaa acagattcac ccctcaaaaa ttatgtgatc
    25381 cgcttttagc taaataccat aagcatatta ataaaaagac aaaaagaaaa cggcaacact
    25441 aatgatgaca ccattttcga ttctatattt attcttaatt aacttatcta tttaacgtaa
    25501 aagtacacac ttattgaaca taaattttta cattatataa ctataacaga atatccattt
    25561 catttcaagc ttttatactt ctcagaatgt aatatccttt atctttattt accacttcta
    25621 catttccaaa aagttcattc attcttttct ttgcagatgg cataccttgc ttcttctgaa
    25681 ttacaacgaa tagttcaccg ttcgagtcta atctatgtaa tgcttgctcg aatatacgat
    25741 gcacggtttc tttccctgct cttattggtg gattggttaa aataaaatca aaacttttgt
    25801 cttccacagc agacaaagca tcactttcct ttacgatcac attatcaata ccatttaatt
    25861 ttttgttttt ttcaactaag gctagcgctc tgtgattaac atctagcatt gtaattgaat
    25921 gatgtggtga tactttagca atcatcaaac caattggtcc gtaaccacaa ccaacatcgg
    25981 caattcgctt acttggacca ggtggatgcg cttttaaaaa agtttgaaca agaacatctg
    26041 aaccataatc tactttatct ttcgaaaaca ctccgttatc agttattaaa tcaattttat
    26101 gatggtttaa ttgatattga atacgttgtt cattgctaat tacacttgga tcttcatcgt
    26161 aataatgact cattattcca cctcatcttc actttgtaac tcgtcattat aattataaca
    26221 tttcactcag tctttaaatg aataaaaccc cgttatcaaa ataacggggt tttaacttct
    26281 caaagaatca ttatattata gcaatacttg ctataaaact ttgattatta agtttaagat
    26341 acttgaatta ttttaattct acagtagctc caacttcttc taattgttct ttaagttttt
    26401 cagcttcttc tttaggtaaa gcttctttga ttactttagg agctccgtct actaattctt
    26461 tagcatcttt taatcctaaa ccagttgctt ctttaacagc tttaacaact ttgattttag
    26521 atgaaccagc tgaagttaac tcaacgtcaa attcagtttt ttctgctgca gcgtcagcgc
    26581 caccagctgc acctgctact gctactggag cagctgcagt tacaccaaat tcttcttcaa
    26641 ttgcttttac taagtcgttt aattctaata ctgacatttc tttaatcgct tcaatgattt
    26701 gttcatgatt agccatttta taattcctcc attattttta attttttacg ctaaaaatta
    26761 ttcagcgttt tcttcttttt gttctccaat agctttaacc gcataagcga agttgcgtac
    26821 aggagcttgt aatactgata aaagcataga tacaagacca tcgtgtgaag gtaatgaacc
    26881 aacagtttta acttcttctg ctgtaataac attgccttcc ataacgcctg atttaatttc
    26941 taatgcttca tgatctttag caaatccaga aattactttc gctgcagcta cagcatcttc
    27001 acttgaagtt gcaatagcag taggacctgt taagaattca tctaagcctt cgataccagc
    27061 tttttcagct gcacgacgta ccatagtgtt tttgtatact ttatactcaa caccagcttc
    27121 acgtaattgt gaacgtaagt cagtaacttc agctactgtt aatccacggt agtcaacgat
    27181 tactgttgaa actgaatttg atagtacctc agcaatttca tcaactagtt gttttttagc
    27241 ttcaatgata gcagacattc agacacctcc atttaaattt ttggtgcttt ttttaaaatt
    27301 aataaaaaaa gcactttcta cccacggcaa aaagtgcttg aaagatcata gatcatcacg
    27361 ttcaagtcaa ttttagcctc ggcaggataa attttaagtt ataaataact cctactgtct
    27421 taggtaaaat aatcacaata agtaatataa cttggaaatt acttattgtc aatatatttt
    27481 tacagactga aagatatttc tttcagcctg taattgttta tatcatttat tatttgaaac
    27541 ttgcagtatc aattttaact ccaggaccca ttgttgtagt tacagcaaca gatttgaagt
    27601 atgtaccttt aacagatgat ggtttagctt tagctaatac atcttgtaaa gtattgaagt
    27661 tttcaattaa ttgttcatca gtaaatgaaa ctttaccaat tgatgcatgt acgataccag
    27721 ctttttcagc acgatattct actttaccag ctttgatttc ttcaacagct tttttaacat
    27781 ccattgttac agttccagtt ttagggtttg gcattaaacc ttttggtcct aatacacgac
    27841 ctaatttacc aacttcaccc atcatgtctg gtgtagcaac tactacatcg aagtcgaacc
    27901 aaccttgttg gattttttga acgtattctg cttcacctac atagtcagca cctgctgctt
    27961 cagcttcagc aattttgtca cctttagcga atactaatac actttgtgat ttaccagttc
    28021 cgtttggtaa tacaactgca ccacggattt gttggtcatt tttacgtgta tcaattccta
    28081 aacggaatgc aacttcaaca gaagcgtcaa agttagcaat gcttgtttct ttagctaatt
    28141 taattgcttc ttcaacactg tagtgctgag tacggtcaac tttactagct gcttcttgat
    28201 actttttacc ttttttagcc atttatagtt cctcctttag tggttttagc ggaatttcct
    28261 cccacattac ttgctatatg caagttaaga gcagacaaca gaagaattaa tcttatgaat
    28321 aaatcaatca ttaatctagt cattcagatt atatttataa ttcatcatta ttatctctgt
    28381 tatctgcttc atcgtttaaa tcatgtaatc aaatttaata ttcgtaaaat tattctacaa
    28441 cgatacccat actacgtgca gtaccttcga taatacgcat agctgcttct tcgtcagcag
    28501 cgtttaagtc ttgcattttg ctgttagcaa tttcgcgtac ttgatcttta gttactgtag
    28561 caactttagt tttgtttggt tcgcctgaac ctttttcaat accagctgct tttttaagta
    28621 atactggagc cggtggagtt tttgtaataa atgtaaatga acgatcttca taaacactga
    28681 tttctaccgg aataattaaa cctgcttgat cttgagtacg tgcattgaac tctttacaga
    28741 atcccatgat gttcacacct gcttgaccta atgctggacc aactggtggt gctggattcg
    28801 ctttacctgc aggaatttgt aatttaacaa ctttatctac ttttttagcc acgatgtgca
    28861 cctccttgat atcgtgatgt ggtcacaggg ctcagttttg ccctcccact cttaacattt
    28921 cgtgacgaaa tgggcgctct aaaagcgcga ccacataatt ttaacacttt tctaattaaa
    28981 aaatcaatac tcttttttat cttttggtta gtttaataac tttaattgtt aattaaagct
    29041 tttctatttg atcgaattca acttctactg gtgtttctcg gccaaacata tctactaata
    29101 ctgttagctt aaacttatct gtttcaattt cttgaacttc accaacttga ttcgcaaatg
    29161 gacctgattt aatacgaact tgctcgccaa cttcgagttc aacatcgata gtcttttctt
    29221 taagacccat ttgttttaag atgaagcgaa cttcttctgg taacaatgga tttggcttag
    29281 accctgcacc tgcagaacct acaaaaccag taacgcctgg tgtatttctt accacatacc
    29341 atgattcatc tgtcatgatt aattccacta aaacatatcc agggaatgtt tttttaacag
    29401 tcgttttagc tttgccatct tttacttgag tttcttcttc ttccggtatg actactctaa
    29461 agatttgttc agtcatattc atagattcta ctcttttttc taaattcttt ttaactttat
    29521 tttcatatcc agaatatgta tgcactgcat accaacgctt tgcgccaact tcttcagaca
    29581 tgtcttcact cctctaacga aataataaat ttttcaatgc tgtaattcct aaatctaagg
    29641 catagaaaaa gactaagaag aatataacag tagaaactac aattacagta tatttaaata
    29701 gctcttcttt cgtcggccaa cttgtttttt ccatttcaga cttaacgcct ttaaagaaac
    29761 tttctttttt agccatctgc tcaacctcca aatcctttga acatattatc aaagtattat
    29821 ttcgaatgta tttatttcga ttctttatga attgtgtgcg cgttacattt tggacaatat
    29881 ttctttaagg ttaatcttgt tgccgagcct tcttgcttag gaacattata atttctattg
    29941 ccacaagctt cacaatttaa aggtattttt ctcacaataa ttcaccttta ttcattataa
    30001 taccagtata ctatacataa aatttataac aaatgtcaaa ccatcagtaa gtaacacgtt
    30061 ataattcaat tcacgtccta aggcgctttt caaatcattt tgaaataacg ttttatttta
    30121 tttttacatc gttgtatggc attataaatc actttctctt ttacatgcat caattgagca
    30181 atttctctcg gcttatattg ttcacacatt aaatacatga catctttttc aaatgcactc
    30241 aaatacttaa actgacaaat caactcttta attgctaatt tattcaaata attgttttca
    30301 acatcaaaac gctttattgc acatggatac gtaactctat attcattaat caaattatcc
    30361 attcttttat ttgtagctaa atattttctc aaataatcat actttacaga cgtaattaaa
    30421 cattgaacat aatttgtgaa aggctgctct ccactaaaat caaatgtttg gaccgaccta
    30481 tacatccgta taagtatttc ttgatataag tcttctaaat cataatgatt aaatccaaat
    30541 gttttgatgc gtcgaataat taatggttgt agatccatta ctaacttttc gaagtcttta
    30601 tcatttattg cattgttacg tttgattgta ctgtcttgag ttgtcaaatc gtatttcaaa
    30661 tattaaacta accccttcta tctaaaattt aaggttagtt taatattgtt acattcaaaa
    30721 tttcaagatg acggaaatgt catttcttat gatgtcctct tcgtattttt tcaaattctg
    30781 caaggatttc agaagataac ggaattcgag ttcttggctt gttttcactt atatcatcta
    30841 atgatttact cacatcaatt tcattttctt ttaaatctct ccacatttcg cgagatgata
    30901 ttctatatgc acctgatcca aagatagcat gttgctcact catatcactt gttacaactg
    30961 taatatgctt agtatgcttg tcataaagtt cataaacata acgttctatg aaactatcag
    31021 ctgtttcttt ttccttggta aaaatcgttt taacgccatg atacatgtat tctctttcaa
    31081 cacccgattg gtcataagca tcgaaaacac aaataatttc atctgaaata actgcattat
    31141 aatttgcaat tgcatctatt aattgcattc tagcttcttc taaattctct tttgcaatgg
    31201 cgcttagcgt tggtgattgt cctatcatat tgtatccatc aatgattaag taacgttctt
    31261 tcattatatt tctccaacat catgtctttt tcgaaatact tcgtacatca ttaaacttgc
    31321 tgcaaccgaa gcattcaaac tgtttacatg tccaaccatt ggaatcttaa tataaaaatc
    31381 gcatttatca cttactaggc gactcatacc ctgtccttcg ctaccaatta caatagccaa
    31441 tgacatgtcc gcttctagat ttctataatc tgttgcatta ttagcttcag tgccagctac
    31501 ccaaaagcca ttatctttta gttcatcgat agttttagct aaatttgtca ctcgaataac
    31561 tggtacatgt tcaattgcac ctgttgaggc ttttgcaact gtttgcgtta gtgtaactga
    31621 acgacgttta ggaataataa caccatcaac tcccgttgca tcggctgttc ttaaaattga
    31681 tcccaagtta tgtgggtctt ctaagccgtc taatataagt actgtcaata aaccttcttt
    31741 ttctttttgc tgttttaaaa attgatcgaa gtcagcatat tcatatggtg caataagcgc
    31801 tgcaacaccc tgatgtggtg catttgctaa aaaatctaat ttagattttg gtacagtttg
    31861 aacaatgatt ttttgatctt ttgcattttt taaaatttca ttaatttgtt gctttttaat
    31921 accttcttga atcaatatct tatttatcgg atgcccagta ataatcgctt ctctaacagc
    31981 atgcctacca acaataaccg tatcttccac atcgtcacta cctttcgttt actattgtta
    32041 ttattttatt taataatgcc tctaatcgtt cttctctttt ttctaaataa agaaaaccta
    32101 tcactgcttc tatcgctgaa cttttacgat atgtttgaac atcagtgttt ttagctttag
    32161 tatgactttt cgcgttacgc cctcgcttca aaatatccat ttcttcgtct gtaaaccatt
    32221 cttgctccat taaatattct aacgtttgcg cctgactttt ggcagataca tattttttag
    32281 acatttgatg tagtttatta ggcttacttt taagctttaa aacgatatag gtacgtacat
    32341 attgatctaa gactgcgtct cccatatatg ctaaggtcaa tggattcaat aatttaatgt
    32401 gattatcttg ttgattatcc acgtttaaat ctaacccctt gaggtgtgtc ttctaatata
    32461 atgttttgtg atttcagcat gtctcgaatt tcatctgctc gtgcaaaatc tttgtttttc
    32521 cttgcttcat tacgctcttc gattaatttt tcaacatctt catccaataa ttcatctgca
    32581 tttttagatt ttaacggtac acctaaaaca tcgctgaaaa tttgataaac tgctttaaat
    32641 ttatcaatta cttctgttga tgttgtgttc tctagtacat atttattcgc aagttttgct
    32701 aaatcatacc aagctgtaat tgcattagct gtattaaaat catcattcat aactgtttca
    32761 aaacgattta aaatcgcatc aatttgatca atatatgtct gttgattttc aatattagta
    32821 gtaatttgtg cgcgctcttc aattaattga taactattgc gaatacgctc tagtccacta
    32881 cgtgctgatt ctaccaattc tagattatag ttaattgggc ttctataatg tacgctaatc
    32941 ataaagaatc ttagtacatc tggatcaact tctttaataa tatcgtgaac taaaataaag
    33001 ttgcctagtg atttactcat tttttcatta tcaatattaa tgaaaccatt atgcatccaa
    33061 taattagcaa atggcgcatg attatgtgct tctgattgtg ctatttcatt ttcatgatgt
    33121 ggaaattgta aatctgaacc acccgcatgt atatcaattg taggtcctag ctcatgaaat
    33181 gccattacag aacattctat atgccatcct ggtctacctt caccaaatgg gctatcccaa
    33241 ctaatctcgc caggcttcgc ttttttccac aatgtaaaat caagtgcatc ttctttatgc
    33301 tctcctgcat ctatacgagc acccactttt aagtcatcta tggattgatg acttaattta
    33361 ccataacctt caaatttacg tgttctaaag taaacatcgc caccactttc atatgcataa
    33421 ccttgatcca ccaaatcttt aataaattga ataatgtcat ccatatggtc cattaccctt
    33481 ggatttgaag tcgcttttct aacatttaac gcaccaacat cttcatgaaa agcagcgata
    33541 tatttttctg caatttcggg aacagactga tttaattctt gagaacgttt aattaattta
    33601 tcatctacgt ctgtaaaatt tgatacatat tctacattat atccttggta ttcaaagtaa
    33661 cgtctcacta cgtcataatt aattgctggt cttgcgttac caatatgaat gtagttatat
    33721 acagtaggac cacatacata catttttact ttccctggtt ctataggctt gaacacttct
    33781 ttttgacgtg taagcgtatt atataatgta atcatcttga atctctccat tcctagtctt
    33841 ttcaagttgt cgttctaaat gcttaatttg ttcataaatt ggatcaggta gatggcgatg
    33901 atcaaatgtt tttccaactc gaacaccatc ttgcttaaca atatgtcctg gtataccaac
    33961 aaccgttgaa taacttggaa ctgattgtaa aacaactgaa tttgcaccaa tatttacatt
    34021 tgaatttatt ttaatatttc ctaaaacttt cgcaccggct gctattaaaa cattgtctcc
    34081 tatatctggg tgtcttttcc ctctttcttt ccctgtccca ccaagtgtca cgccttgata
    34141 gattgtcaca ttatcaccaa ttgtacatgt ttctcctatt acaacgccca taccatgatc
    34201 tataaataga cgctttccaa ttttagcacc tggatggatt tctatacctg tgaaaaatct
    34261 tgaaatttga gatatcgcgc gtgctgcaac atattttttt tggttgtata acttatgtgc
    34321 aatcaaatga ctccaaactg catgtaaacc tgcatacgtt gtaatgactt ctaatgttga
    34381 acgtgccgct ggatcctgct caaataccat ttttatatcg tctctcattc tttttaacaa
    34441 gatcatttcc tcctcaatga ttgaactacg taaatacata attgaagtac ctgcgaaatt
    34501 aaatatcaaa aaagcaccac taacatacaa gttgtattgt tagaggcgct tccgcacggt
    34561 tccactctga atttagcgaa taacattaat aatattgcgg gcgcttccaa attatcaagg
    34621 aaactaagtc aacttaatgc tcatcactct cattatatat ttaattcatt ttacgaaggt
    34681 gcattcatta atttctacgt tgtactcaca gcaaccgtac actctctgca tcgtataaat
    34741 ttaattacta atccttcgtt ttatataaaa tactttattc atattgttgt aatttgttaa
    34801 tacaacttta tcatatgtgg ttacgttata aaattcaagt atatactacc ttgatcttgt
    34861 ctatttcatt acttatattg ttttaaacgg tttagcactt tttctttacc aagtacttca
    34921 attgtatttg gtaattcagg accatgcatt tggcctgtta cagcaacacg aataggcata
    34981 aataattgct tgccttttat tcctgtttct ttttgaactt ctttaattgt ctttttaatt
    35041 tcagccgctt caaatggttc aagtgcttct aatttactga ataagtgcgt cattaactct
    35101 ggtacttgct ctccattaat cacttgttgt tcttcttcac caagagctgg catttcttta
    35161 aagaacattt ctgataaagg tacaatttca ccggcataac tcatttcttt ttgataaagc
    35221 gcaattaatt tgcgtcccca agataaatcc tcttctgacg gcacctcagg aatcaaattt
    35281 gctttaatta aatgaggtaa tgctaattgg aatactgttt cagtatcttt ttgtttcata
    35341 tattggttat taacccatgc taatttttgc ttatcgaaaa atgctggtga ttttgacaaa
    35401 cgcttttcat caaagatttt gataaattct tctttagaaa agatttcttc ttcaccttca
    35461 ggagaccaac ctaataacgc aataaaatta aataacgctt caggtaaata acctaagtca
    35521 cgatattgct caataaattg taaaatttgc ccatcacgtt tacttaactt tttacgttct
    35581 tcattaacaa ttaatgacat atgaccaaaa cgaggtggct cccagccaaa tgcttcataa
    35641 atcataattt gtttaggcgt gtttgaaata tgatcatcac cacgaattac atctgaaatt
    35701 tgcatgtaat gatcatctat agctactgca aaattgtacg ttggaatgcc atcttttttt
    35761 acgataaccc agtcaccaat accatttgaa tcaaatgaaa tatttccttt taccatatca
    35821 tcaaatgaat acgtttggtt ttgaggtact cggaaacgaa ttgatggttg gcgtccttct
    35881 gcttcaaatt gttgacgttg ttcttcagtc aaatgcgcat gttgaccacc atagcgaggc
    35941 atttcaccac gagcgatttg cgcttcacgt tcagcttcta attcttcttc tgtcatatag
    36001 catttatatg ctttatcttc tgctagtaac tgatctatta atggttggta gatatgttga
    36061 cgttcagatt gacgatatgg tccgtagcca ttgtctttat ctacagactc atcccaatct
    36121 aatcctaacc atttaagatt atcaaattgt gatgtttctc catcttctaa attacgtttt
    36181 ttatcagtat cttcaattcg aatcacaaaa tctccgttgt aatgtttagc atacaagtaa
    36241 ttgaataatg ctgttcttgc attaccaata tgaagatacc cagttggact tggtgcatat
    36301 cttactctta tacgatcgct catttttttc actcctaaat taaatatcag attttcaagt
    36361 tagttcatat aaattgttca tttgctatct tcgaccgtca taacaaatgt ctaactcgtc
    36421 ttattgttaa aacgaaacaa tgctttttaa catgacctta aaataatttc attgtttaat
    36481 cataacataa ttccctgggt aatatgctta aattttaaat agaaagctgt tgttttttca
    36541 acactttaaa aaagctatcc ctaagaatca agactgcact acataaacat ttcaacgact
    36601 taaaaatgaa tattatgtac tgtgaaatta aacttcaaat taactattca aatacgttaa
    36661 aattgattct aattttgtat gtcttgattg ctataagaat aactttatta atatctaaaa
    36721 tttaacactt aatgaacttg tttcaatgat atattagcac tatttgtatt ttttgataac
    36781 taatatgttt tgcatttatt tatagttata cttcaaatta caaacttcgc catttcatat
    36841 accttttaat atctattttg ttttcgtcaa ctacagtttt tataatgata ctgtatcttc
    36901 gattttttta gcaaaaacaa ttcttcctga agatgtttgc aataagctga ctacttctaa
    36961 attgacatga ctgccaataa gatttttagc attatcaaca actaccatcg taccatcatc
    37021 tagatatcct actgcctgac caggctcctt acccattttt gtcagtaaaa tatgcagttg
    37081 atcaccttga tgtacattag gtttgattgc ttctgataaa tcattaacat ttaatgcttt
    37141 gataccatgt acatgacaaa ctttatttag gttgaaatct gtcgttataa tacttgcatg
    37201 atattgtttt gctaatttta ataacatcgt atcaatatca ctatgtgttt tagttggatg
    37261 tataaccttt gtaggatagt ctaaatcata caattcattt aaaatatcta agcctctttt
    37321 acccttttca cgtttaacac tgtcatttga atctgcaaca atttgtaatt cattaataac
    37381 accttgtgga attaaaatat tgccatcgat aaaaccgcaa cgaatgactt ctaaaatacg
    37441 accatcaata attgcgcttg tgtcgataat ttttggcgta gcacttttag tatgttgtga
    37501 catggaacgc gctatattct caggtaaaaa cattaacatt tcatctcgtt ttttaaggcc
    37561 aaattggaaa ccgaaataac atagtaatat cgtaattatg acaggaatga aatgattaaa
    37621 aatagagttg ccaattgatt ctaatataaa cgacaccata acagaaataa gtaatccgat
    37681 tattaaacct attgttgcga atagtatttc aacagcactt ctacgcataa taaaatgttc
    37741 taaacctttt atagcgttag taactcgtct aataaataca ccaaaaatta agaacataaa
    37801 aatactaccg ataatgccat ctacatagtg attttttaaa aagctggagt tttgtaatcc
    37861 aagatcattt gcaatttcag gaataataat tattcctaat gcgctcccaa taattaagta
    37921 aataataata accattagtt taacgatatt cacacaatgt cctcctttct tgatgtttta
    37981 tgaatgaaga gcaaatgaca atacttcatg tacagtagtt acacctatta cttgtatacc
    38041 ttcaggatat gtccatccgc ctatattatt tttaggaata attacacgtt tgaaacctag
    38101 ttttgcagcc tcttgcacgc gttgttctat ccgagataca cgacgtacct cacccgttaa
    38161 accaacttct ccaatatagc aatctaatcc gtcgacagct ttatctttaa agctagatgc
    38221 agttgctaca attacactta aatcaactgc tggctccgtt aactttacac cgccagctac
    38281 tttgatataa gcatcttgtt gttgtaatag ataattttct ttcttttcca aaacagccat
    38341 caacaaactt aatcgattat gatcaatccc tgttgccatt cgtctcggat tgttaaaagt
    38401 cgttggagtt accagcgctt gaacttctat taaaagtggt ctggttccct ccatggttgc
    38461 aacaattgtt gaacctggaa catttgttga acgttcttct aaaaacattt cagatggatt
    38521 atttacacct tttaatccac tttgcttcat ttcgaagatt cccatttcat tcgttgaacc
    38581 aaaacggttt ttaacagctc gcaaaattcg atatgcgtgg tgttcatcgc cttcaaaata
    38641 aagcacagta tcaaccatgt gttctagcaa tcttgggcca gcaatttgac cttctttcgt
    38701 tacatgaccc actataaaag ttgcaatgtt catttgttta gcaatattca ttaaactttg
    38761 tgtactttca cgaacttgtg aaacagaacc tggcgcagag ctgatttcag gatgatatat
    38821 tgtttgaatc gaatccacta ctaataaatc aggttgttct tcttttactg tttgataaat
    38881 aacttcaaga tctgtttcag ctaatacttg caattcactt gaatcttcat ctaatcgctc
    38941 tgcacgtaat ttagtctgac taagcgattc ttctccagta atatatagta cttttttctt
    39001 ttgagataac gatgcacaaa tttgtaaaag taacgttgac ttaccaatac ctggatcccc
    39061 accaataagt actaacgatc cgctcacaat acctccacct aatacacggt tgaattctgc
    39121 tgaatctgtt aacactctcg gcgttgtttc atgtttaata ctatttaatt tttgtacttt
    39181 acctgctaat tccttggttt taactccatg tttaggattg gctgcttttt caacaatttc
    39241 ctccatttga ttccaagcgc cacaattagg acatttcccc atccatttag gagattgata
    39301 accacaagcc atacattcaa aaatcacttt tttcttggcc aaaattgcac ctccactttc
    39361 ctttgaacaa atctatttta aacgcacatt cccattatga caaattaaat gtgagttaca
    39421 tttgtttgta ttttaacatg actactaacg caaactgttt taccagattt attttttatg
    39481 aatattataa aataaactta catcatgttg atagtcggta tctttcaaag ataaaatctt
    39541 aataatttct tagtaaactc ttttctctag atttatcaca atattatata gacctatttt
    39601 attttgacgt aagttgctag tatcttcaaa caaaaaacct ttataaaaat tcataccttt
    39661 atgctatcgc tgtaggctca ttaacttgtt acatataatt cttaactatc ctttgatgat
    39721 tgttttatta gattgtttcg ttgatggata ctttcacgaa tttctatagt tcaatgctac
    39781 taaaaaaaca gcccaaaact ttaatttgtt ttggactgtt ttataattat gcttgcgatg
    39841 gtgttttagt ttctgaagtt tgttcagcaa tgtcatattt aaactcttta ccatcatgat
    39901 ctactgtaac tttcttacct tcaatttgat taccatctaa tattaattca cttaaattat
    39961 cttcgatagt tttttgtatc gctctaatta atggtcttgc accatattct ggatcatatc
    40021 cttcttctgc gattttgtct ttcgctttat cagttacaat aatatttatg ttttgttcag
    40081 ataatcgatt tgttaattta ttaaccatca ttgttacaat ttcttttaat tcttcttttg
    40141 ttagtttatg gaatacaatg atatcatcta cacggtttaa aaattctgga cggaatgaat
    40201 tttttaattc ttttaacatc gtttttcgaa ttgtttcata atcttgtcca tcacttgaac
    40261 caccgaatcc agcaaatcgt tgatcttgta attcttgtgc cccaacgttt gatgtcatta
    40321 tgataattgt atttctgaaa tcaactgtac gtccttttgt atctgtcaaa tgtccatcat
    40381 ctaaaacttg taatagaata ttaaatacat ctggatgagc tttttcaatt tcatcaaata
    40441 aaattacaga atatggttta cgtctaactt tttcagttaa ttgtccacca tcatcatgac
    40501 caacatatcc tggaggagca ccaactaatc ggctcactgc gtgtttttcc ataaattcac
    40561 tcatgtctac acggatcatc gcatcatcat cgccaaacat tgattcagct aaagctctag
    40621 ctaattcagt tttaccaaca ccagttggtc caaggaagat aaagctacca attggtcgtt
    40681 taggatcttt taaccctgca cgggcacgtc taaccgcttt actgattgaa ttaacagcat
    40741 ctttttgccc aataactctc tcatgtaatg tatcttctag actaagaagt ttttcagatt
    40801 ctgtttcatt gattttagtt aatgggatac ctgtccatcc tgcaataact tcagcaatat
    40861 cttcttctga caatgaagtt gacatgccat tttgtgcatt cttccattca tttttagctt
    40921 cttcatattg cttttcaagt tttgtttgtt tatcacgcag gttagcagca ttttcaaact
    40981 cttgagcatg tactgcggca tctttttcat ttttaacttt ttcaatttct tgttcaattt
    41041 cttttaaatt attaggtgtc gtatgactct taagtcttac tttagaactt gcttcatcaa
    41101 ttaaatcaat tgctttatct ggtaagaaac gatctgaaac gtatctgtta cttaatttaa
    41161 cagctgcttc aatagcttcg tctgaaatat taatacgatg gtgtgcttcg taacgatctc
    41221 ttaatccttt taaaatagca actgtatcta ctactgaagg ttcatcaact tgtacaggtt
    41281 ggaaacgacg ttctaaagcc gcgtcttttt caatattttt gcgatattca tctaatgtag
    41341 tagcaccaat acattgtaat tcaccacgtg ctaatgccgg cttcaaaata ttcgaagcat
    41401 cgatagcacc ttcagcacca ccagcaccaa ctaaagtatg caactcatca ataaatagga
    41461 tgacattacc tgcttgttgg atttcttcca taaccttttt cagacgctct tcaaattcac
    41521 cacgatattt agtacctgca actactgttc ccatatctaa agacataaca cgcttatctt
    41581 ttaatgtctc tggtacctca ttattcacta tggcttgcgc taaaccttca gcaatagcag
    41641 ttttaccaac acctggctct ccaataagca caggattgtt tttcgtacgt ctacttaata
    41701 cttcaattac acgtgtaatt tctttatcac gtcctataac aggatctaat gtaccgtctt
    41761 tggcaatgac tgttaagtca cgagctaaac tatctaaagt tggagtatta tttgacttac
    41821 tagcttgtgc atttttatta ctcatttcag ggtttcctaa agctttcaca acttgtgcac
    41881 gtgctttagt aatatttaaa tctagatttg caaaaactct tgctgcaaca ccttcatttt
    41941 cacgaatcaa gcctaataaa atatgttccg ttccaacaaa attgtgatgt aattttctag
    42001 cttcatccat cgataattca atgacttttt tagctctagg tgtataatgc aatgtaccaa
    42061 catgatcttg accatgtccg attaattttt caacttcttc aattacttta tcttcagtga
    42121 tattaaaact ttctaatact tttgcagcaa ttccttcagg ttctttcatt aaccccaata
    42181 ataggtgttc tgttcctata tttgaatgat ttaaacgaat tgcttcttct tgggcatgtg
    42241 ctaatacgcg ctgtgcacgc tcagttaatc taccaaataa cataaataat gacctcctac
    42301 tttatatgtt ctcttagtat atctgctcgt ttttctttta cagatttgtc atcttcttca
    42361 tctaataaaa atggtgactg tatagctacc atcaattcat taaatttaaa gttttgtaat
    42421 tcaatgtaat ttaaatctat accaagttta acttcgctta atctataaga agcctcttcc
    42481 atagttatca ttctacagtt ttgtaaaata cctagcgagc gaaaaacacg gtcttgtgtt
    42541 tctaattgat tataagtgtc taacttttgt cgtatttgtt tttcttcatg aatgatttga
    42601 ttaacaactt ctgttaatgt ttctatgatt tctaactcag atttaccaag tgtaagttgg
    42661 ttggatactt gataagtatg tccataaact tgcgaacctt caccgtaaat acctctgatt
    42721 gtatatccaa aacgattaat ggtttgagca atccgtgtca ttcttttcat aatagataga
    42781 cctggcaaat gtagcatcac gcttgctctc ataccagtac ctatattggt aggacatgta
    42841 gttaaataac caagttgttc atcataactt atatcaaggc ttcgatctaa ttcatcatca
    42901 attgatgaag cttgattata taaagcctgt aatgtcgtgt cagttcccat agcttgaata
    42961 cgaatatggt cctcttcatt tatcatgaca cttaaagatt catcgtcatt cactaatact
    43021 gcggctgctg gttgttttat tagttcagga ctaatcaaat gctttgcaac cattttcatt
    43081 ttactttgtt gatccatttg atcaagacgc attaattcaa agtttggcaa ggcatcttgt
    43141 acctcattta taactctaaa tccatcattt tcagtagcat acattagtgg atgcacatga
    43201 ttttctaaat tacgcgctaa ccgaattcta gaagacataa caattggtgt ttcttcatta
    43261 cttttcatcc attggctgat attatcatga atattatgcg tcatcatgtt gcacctcact
    43321 ctcagctttt agtgctttaa tttcatctct aacaatggct gcttcctcaa aatcttggat
    43381 ttcaataagt tttttcaaat attcattctt ttcttcgatt tttcgcttta aagctatctt
    43441 tttatgtgaa gaatgtggtg tctttccaac gtgctcaaat tgtccacctt gaactctgcg
    43501 gacgatatca atgatgtcat ctttaaatgt tgcataacaa ttagcacacc caaatttacc
    43561 aacatgtgca atatctttca aagtcatatg acatgatggg caacgttttt cttctttaaa
    43621 cgctacttct tgaaaattaa ttccatgttt cgtagctaaa tgttgtaaaa tttgcttaac
    43681 aacaaatgct tcttcgaaat tatcttgatg ttcttgatat tcaggttgtt cattagcttg
    43741 attccacgga tggtgtccct cagcacaagt ttgacacacc attttttctt ctgttttatt
    43801 tttacttgta actttaactt ttaattccgc ttcattaagt tgacaatttt cacaaagcac
    43861 ttcaacacct catttcattt agtaataatt tataactggt aacaaacgtt ttaaaatatt
    43921 tgctctaata atatctctag aaaccatatc cattgatagc gtttctctat caataactgc
    43981 ttgaatcatt ttagcttcac gttcatttat taacatttta tctaaaagcc catcaataat
    44041 ataataagct tgttgttgag aaatagaagg tccaatcagc tgaagcaaat gattaatata
    44101 acctgttgca tctttatttt caattttagt gattcggatg taaccaccac caccacgttt
    44161 actttcgatt tcataaccat gttcattagt gaatcgtgtt ttgattacat aatttaattg
    44221 tgatggtacg caatcaaaac gctgtgcgat attcgctctc tgaatttcaa cgacatcttc
    44281 attcgactct tcaaataaac gtttgatgta ttgttctatg atgtcagaca tattgtgcat
    44341 gtatatcacc cctttttgac cttctttgac tatattatat gacctatttt gacctttttc
    44401 aattaatttg tttttatatt ttcaaaaatt tatgatgtta gcccttccat tttattgtat
    44461 gatagattgt agaaaaaact aaaggaatga gaaatatatg catattctaa ttgggattat
    44521 cggaattatc ttctttttag cacttgcgtt tctatttagc tcagacagga aaaatattcg
    44581 ctggaaatat gtaggaattt tacttgcaat tcagcttatc tttgcattta tcttgcttaa
    44641 aactacaatg ggaatcaagg ttattggtgg tatttcaaac ggttttaact acttattatt
    44701 aaaagcagca gatggtgtta actttgtatt cggtggtatt caatatatcg atcctaagca
    44761 accaccattc ttcttcagtg tattattacc tatcgtattt atttcagcga ttatcggtat
    44821 ccttcaatac acaaagattt tacctttaat cattaatgtt ttaggattct taatttctaa
    44881 aattaatggt atgggacgtt tagaatcata caatgcagtt gctgcagcaa ttttgggaca
    44941 atctgaagta tttatttcaa ttaaaaaaca attaccatac atacctaagc aacgtttata
    45001 cacattaaca gcttcagcaa tgtcgactgt ttctgcttca attatcggtg catactttac
    45061 cttaattgaa cctaaatatg ttgtaacggc tgttgtctta aacttatttg gtggtttcat
    45121 catcgcttca attattaacc cttataaagt taatgaagaa gatgacaaat tattagttga
    45181 agaaactgaa gaaagacaac aatcattctt cgaaatgcta ggagaatata ttttagatgg
    45241 atttaaagta gctgtcattg taggcgcaat gttaatcgga tacattgcca ttatcgctct
    45301 attaaatgga attgtaagca atatctttag tacagtttca ggtggcgcta tttcttggga
    45361 tttccaaaca ttaattggtt ttgtctttgc accatttgca ttcttagtcg gtgtgccatg
    45421 gcaagatgca gttcaagccg gttcagttat ggcgacaaaa ctattatcta acgaatttgt
    45481 agcaatgcaa gcacttggta aacttagtga tttatctgaa cacgcaaaag gtgttacatc
    45541 tgtattctta gtatcatttg ctaactttag ctctatcggt attatttcag gtgcgattaa
    45601 atcacttaat gacaaaaaag gagatacagt tgcccgcttt ggtttgaaat tattattcgg
    45661 tgcaacacta gtatctttca tttcagctgc aatcgctgga ttctttattt aacaaataca
    45721 tcaacatcga attaacccct tcaaaatttt gactattctc agtctctttg aagggttttt
    45781 ttcttatgta tagctttatt ttttatcatt tttaattttg acataaaaaa taactcctcc
    45841 aaagctaagt ttgaaggaga tattttcttc tcaacaaatc tataaatatc aataattcgt
    45901 tgatttagca tacattaagc tatgcttttt ttacaatatg attaataaag taatcagtta
    45961 ctctatagtc atctgttaat tcaggatgga atgatacgcc taaatattta ccttgctgaa
    46021 cagctacaat tttctcatta accttacata ggatatctac gccttgacct actttttcaa
    46081 tatgtggggc tcttataaag acaccttcaa tatctgtagc gataccttta atatctaatt
    46141 ctgtttcaaa gctgtcaact tgtctaccga atgagtttcg ttgtacagta atattcaact
    46201 tgttaaggta tccttcttca ccaactatat cttgcgctag aactattaat cctgcgcatg
    46261 taccaaacat aggtaaagtt gaattttgta aagcctcttt aaatccatat aaattcatta
    46321 atcgacgtaa cgttgtagac tcgccaccag gtaatattaa gccctcgatt tcttctaatt
    46381 gttcaacttt tttaactgca ataccttcat gaccacttaa ttcaatatgt ctaatatgtt
    46441 cacgtactgc accttgtaat gctaatacac ctattttcat atcttaccaa ccacgctctt
    46501 gcatacgttc ttctaatgat aattgattga tatctaaacc tttcatagca gtgccaagtt
    46561 cacttgctaa tcttccaatt agttcatagt cttggtaatg tgttgttgct tgaacaattg
    46621 ctttagcaaa tttttctgga tcttctgatt taaaaatacc tgatccaacg aatacaccgt
    46681 cagcacctaa ttccatcatt aaagcagcat cttgaggagt cgcaacgcca ccagctgcaa
    46741 agttaactac cggtaaacga ccattgtctt taatttgttt taaaatttca taaggcgcac
    46801 cgatatcttt cgcaaaagtc ataatctcat catcattcat tacagtcaat cgactaactt
    46861 ctgaattaac ttgtctcata tgtcttacag cttcaacaat attacctgta cctggttcac
    46921 ctttagtacg taacatagca gcaccttcac caattctacg cgcagcttca cctaaattac
    46981 gacatccaca tacaaatggt actgtaaatt gatcttttct taagtgatat tcctcatctg
    47041 ctggtgttaa cacttctgat tcatcaatat agtcaacacc catcgcctct aatactcttg
    47101 cttcagtgat atgaccaata cgtgctttag ccatgactgg aatagaaaca gcattcatta
    47161 cttcttctac aattttaggg tttgccatac gtgcaacacc accagcagct ctaatatcag
    47221 aaggtactcg ttctaatgcc ataactgcta ccgcgccagc ttcttctgca attcttgctt
    47281 gctcagcatt aacgacatcc ataataacgc cgcctttttg catttcagcc atacctcttt
    47341 tgactctgtc tgatccaata attttactca ttatacataa aacccctttt caagttgatt
    47401 aaatacattg taatgtataa actgatattt aaaaaggtac aaaataagat aaatttagga
    47461 ggtcagttat aaaattgaaa aacactttat accatcaact ttatgaaaaa ttaaaaaaac
    47521 aaataattga aggtcaattt aaagaaggcg ataaatttta ttctaaaaga caattaagca
    47581 aacatttatc tattagccaa acaaccgtag agcatgcata tcaacttcta ttagacgaag
    47641 gttacattta ttcaagacct cgctctggat attttgtgtc cgaaattgaa tctttaacaa
    47701 ttttaaacaa tcaacctatc ccctctcttt ttgatgatga ttcatataaa ccaaaagcga
    47761 gtgatgaagc ttatgattat gcttttaatt tggatgaaat agatacgaaa catttcccta
    47821 tagaactttt cagaaagtat tccaaagatc tttatgatac aaatcattta aatcaattac
    47881 gtagaggaca ttttcaagga gaattacatt tgcggtttca actggcattt tatttattta
    47941 ctaatcgtgg cgtaatttgt gatcctaatc aaattataat tggctcatct accgaacaat
    48001 tagtaaatca attagtcgat ttactttata cttcaacatt tattatcgaa aaacctagtt
    48061 atccaccaat caaaaatatt ttagataaaa aacaagttga atatgaacaa attgaagttg
    48121 aagataatgg aataaacgta gatgaagtta ttaaatcaca aaaaaatata gtatatataa
    48181 cgccttccca tcaatttcca actggatatg tgatggactt aaaaaaacga actcaattaa
    48241 tacaatgggc tcaagaaaaa gaagaacgat ttattataga agatgattat gattccgagt
    48301 ttagatattt cggcaaaccc attccagcaa ttcaaggtct atattcaaga ggagaaaaag
    48361 tgatttatat cagtacattt tcaaagtcta tttttcctag ctgtcgagtt gcatatatgg
    48421 tgttacccta ttctataatg aaaaaatatc attctcaaaa tcacattgaa ggcaacactg
    48481 tacctgttca tatgcaaaac cttattgcaa cgtttatatc ttccggtggt tttgaaaggc
    48541 atttaaataa aatgagacga atttatcgta gaaaactaac ctatatttta aaaagattga
    48601 aaccttacaa agagcagctt gatattcaag gtgctgaaac gggtatgcat tttacaatta
    48661 cagtaaaaaa tggattaaca ttacaagagt gtcttgatag agcaaacaaa gtaaagttaa
    48721 aattacaagt ttataatttt gatgatgatc aagactataa gaaaacccct aaatttatat
    48781 taggctttgg cggtatagac gatgatgctt taaaaccgca tgtagacgct ctaataaaat
    48841 cattaattat aaagtaacac ttctttga
//
LOCUS       DALAGF010000006        48756 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.006, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000006 DALAGF010000000
VERSION     DALAGF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 48756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48756
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.006"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            145..1200
                     /gene="lukH"
                     /locus_tag="O2692_000312"
     CDS             145..1200
                     /gene="lukH"
                     /locus_tag="O2692_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HCZ4297962.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKDKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKT
                     VKVVDKYSDDNKPYKEG"
     gene            1222..2238
                     /gene="lukG"
                     /locus_tag="O2692_000313"
     CDS             1222..2238
                     /gene="lukG"
                     /locus_tag="O2692_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HCZ4297963.1"
                     /translation="MIKQLCKNITICTLALSTTFTVLPATSFAKINSEIKQVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKDKMPVTVSEGFNPEFLAVMSHDKKDKGKSQF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLND
                     NEKK"
     gene            complement(2476..3306)
                     /gene="sph"
                     /locus_tag="O2692_000314"
                     /pseudo
     CDS             complement(2476..3306)
                     /gene="sph"
                     /locus_tag="O2692_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(3357..4394)
                     /locus_tag="O2692_000315"
     CDS             complement(3357..4394)
                     /locus_tag="O2692_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCZ4297964.1"
                     /translation="MKTRCYDGKKWQYEFKYEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLHAINNALKSAC
                     RVNNISIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
     gene            complement(4585..5298)
                     /locus_tag="O2692_000316"
     CDS             complement(4585..5298)
                     /locus_tag="O2692_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCZ4297965.1"
                     /translation="MTHNIEKRINKLKTSGNPKFKKLDSDIHYLLKRFEGEKNHKGFY
                     PKFKQGEIVFVDFGINVNKEFSNSHFAIVMNKNDSNTEDIVNVIPLSSKENKKYLKMN
                     FDLKWEYYLRLFLNLISAQNNSAILKEVFDKKYQKNNTEFITKDYFSEFISDSLEIEN
                     KLNKIDRNINNIVSAIDKVKKLKGNSYACINSFQPISKFRIRKVLPQKIKNPVIDSSD
                     IMLLINRINNNILQIPDIR"
     gene            complement(5376..5558)
                     /locus_tag="O2692_000317"
     CDS             complement(5376..5558)
                     /locus_tag="O2692_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297966.1"
                     /translation="MKITNCKIKRETVIYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKIDGDKLTKEDSL"
     gene            complement(5762..6103)
                     /locus_tag="O2692_000318"
     CDS             complement(5762..6103)
                     /locus_tag="O2692_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297967.1"
                     /translation="MIKKIFTKKHVFLVIEDENHNHSDAVFGKSILLSIYVGVNKKTN
                     SKSGKFIYLDRSKRIVRQSDITKIESANENDVDFYNLLKKEKEIVYSKNIVDKYNLAN
                     YIIYYEVSTKE"
     gene            complement(6109..7041)
                     /locus_tag="O2692_000319"
     CDS             complement(6109..7041)
                     /locus_tag="O2692_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCZ4297968.1"
                     /translation="MKKYDIAVLDFETMNEHMNSPCEVAVSLIKDLSIVKVYSSYINP
                     PNNRYNLKNAKIHKIPEDVILKAPKYPDIYQEILYLLKESHLIIAHNALFDISVLKNT
                     NNYYDLPVPNFMYVDSINIFRSFHAISSFKLENLCSLYDIDKEKLHSAKFDVLALSKM
                     LISLAKNNQHYSVLKLIHYMPKQYIRFSKYSNSPTKLFDSGFQKIHMKISEINKIEVE
                     SVIPILKDKNVVFTGNFDTEKQDLMILTRKKGAYIRSDVTAKTDILVEGVQDDKYKDV
                     NGLVSKQRKAREYVGNGAKIQFLNEEDLINLIKE"
     gene            complement(7057..7770)
                     /locus_tag="O2692_000320"
     CDS             complement(7057..7770)
                     /locus_tag="O2692_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4297969.1"
                     /translation="MNKERNIIIAKNIRKFLNDSNMSQKKLAELINIKPSTLSDYLNL
                     RSNPSHGVIQRIADVFEVGKSDIDTTYKDDNDITSIYNKLTPPRQENVLNYANEQLEE
                     QNSKGDNVVDINSYKQEKTPVNVNGCVSAGVGERLHDETLFTEMVKGPIPTHDLALKV
                     NGDSMEPMFKDGEIIFVEKTHNIKNGQIGIFIIEEEAYVKKVFVEDDRLTLVSLNKDY
                     DDLHFYRNESVRLIGKVIL"
     gene            7733..7906
                     /locus_tag="O2692_000321"
     CDS             7733..7906
                     /locus_tag="O2692_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ4297970.1"
                     /translation="MFLAIIIFLSLFILLTSFFLIIRNFHIIVKFFTKKTYLMLTIRK
                     FRIILGLRKAVTT"
     gene            7903..8166
                     /locus_tag="O2692_000322"
     CDS             7903..8166
                     /locus_tag="O2692_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4297971.1"
                     /translation="MKTLKELRTDYGLTQKELGDLFKVSSRTIQNMEKDSTNIKDSLL
                     SKYMSAFNVKYDDIFLGNEYENFVFTNDKKKSIILAFKEKQTS"
     gene            8182..8397
                     /locus_tag="O2692_000323"
     CDS             8182..8397
                     /locus_tag="O2692_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCZ4297972.1"
                     /translation="MNIQEATKLAMEKGISIRRENQDVYGILPTNLQRYQCLVVSRHY
                     KKKRQTAAGRWQPSADDLIADDWILDY"
     gene            complement(8386..8715)
                     /locus_tag="O2692_000324"
     CDS             complement(8386..8715)
                     /locus_tag="O2692_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297973.1"
                     /translation="MTNEAKFVLLQLYSIYLDRIDEGMSKRSASYFGSDESSFNAFFL
                     DFNFEDYIDAVLELKHRDFVIASAEDGGFLEMALSREGIAYSESESKKDYKTLMDLIR
                     DLKKLII"
     gene            8766..9518
                     /locus_tag="O2692_000325"
     CDS             8766..9518
                     /locus_tag="O2692_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCZ4297974.1"
                     /translation="MQALQTNSNIGEMFNIQEKENGEIAISGRELHQALEVKTAYKDW
                     FPRMLKYGFEENTDYTAIAQKRATAQGNMTHYIDHALTLDTAKEIAMIQRSEPGKRAR
                     QYFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVG
                     ELAKIIKQNGVNIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITH
                     SDGHTSISKTPKVTGKGQQYFVNKFLGEKQTS"
     gene            9534..9710
                     /locus_tag="O2692_000326"
     CDS             9534..9710
                     /locus_tag="O2692_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297975.1"
                     /translation="MNEEKLKMILLLLEDIPRDEWNRLVNEVNNQYSYQADKVGLASD
                     NCQQIANNYKHYGF"
     gene            complement(9707..9937)
                     /locus_tag="O2692_000327"
     CDS             complement(9707..9937)
                     /locus_tag="O2692_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297976.1"
                     /translation="MHKRLLTQYLDKEIVTSLDLHLINGEVIKVQEHMKDAESKTLHI
                     IYPKDRVVSLDHVLYFDINVKGEKNNDSPYPS"
     gene            9996..10316
                     /locus_tag="O2692_000328"
     CDS             9996..10316
                     /locus_tag="O2692_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCZ4297977.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDESFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            10313..10474
                     /locus_tag="O2692_000329"
     CDS             10313..10474
                     /locus_tag="O2692_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCZ4297978.1"
                     /translation="MSDTYKSYLLAVLCFTVLAIVLMPLLYFTTAWSIAGFASIGTFI
                     YYKEYFYEE"
     gene            10567..10884
                     /locus_tag="O2692_000330"
     CDS             10567..10884
                     /locus_tag="O2692_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297979.1"
                     /translation="MAENIKTEQHYYTKDFSGYRNEEDNFVANQELTVTITLNEYRKL
                     IEIKAVKDKEEDTYRGKYFAEERKNEKLEKENIKLKNKIYELQNEEDNEEDEENEEEV
                     NND"
     gene            10877..11179
                     /locus_tag="O2692_000331"
     CDS             10877..11179
                     /locus_tag="O2692_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCZ4297980.1"
                     /translation="MTKKYKDMTQEEVKDLLSEKSGELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLASLLDSTKSYKDIVNVLQMCKSQKFHGIDDNKEG"
     gene            11184..11444
                     /locus_tag="O2692_000332"
     CDS             11184..11444
                     /locus_tag="O2692_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCZ4297981.1"
                     /translation="MYYKFGEIKNKIISFNGFEFKVSAMKKHDGISIQIKDMNNIPLK
                     SFHVVDLSELYFATDAMRDVINEWIEENTDEQDRLINLVMKW"
     gene            11458..11820
                     /locus_tag="O2692_000333"
     CDS             11458..11820
                     /locus_tag="O2692_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297982.1"
                     /translation="MAILEDIFEELKLLNNNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     EETAQLESIEEVKETSADLTKDYVLSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEHYEKIVDFMNARINA"
     gene            11817..12983
                     /locus_tag="O2692_000334"
     CDS             11817..12983
                     /locus_tag="O2692_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HCZ4297983.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSTVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDNDVIALFETKLDLGKYVPESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYDIHTIRMTIIQPRIDNFSTEELPISRLLQWGADFVKPLARL
                     AYNGGGEFKAGSHCRFCKINHSCRTRAEYMQNVPQKPPHLLSDEEIAELLYKLPDIKK
                     WADEVEQYALNQAKENDKNYPGWKLVEGRSRRMITDTKAMLEKLVEAGYKPEDITETK
                     LLSITNLEKLIGKKAFSKITEGFIEKPQGKLTLATESDKRPAIKQSAEDDFDKL"
     gene            13009..13566
                     /locus_tag="O2692_000335"
     CDS             13009..13566
                     /locus_tag="O2692_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HCZ4297984.1"
                     /translation="MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQESKYSISLIIP
                     KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFIN
                     ASSKQAPGIIDQNKIRLTDSGTVVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEK
                     GEPLGGASAAEDDFDELDTDDEDFL"
     gene            13634..15586
                     /locus_tag="O2692_000336"
     CDS             13634..15586
                     /locus_tag="O2692_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HCZ4297985.1"
                     /translation="MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAI
                     DMTKVDNEPFHADFETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATG
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGVRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            15599..15784
                     /locus_tag="O2692_000337"
     CDS             15599..15784
                     /locus_tag="O2692_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HCZ4297986.1"
                     /translation="MQHQAYINASVDIRIPTEVESVNYNQIDKEKENLADYLFNNPGE
                     LLKYNVINIKVLDLEVE"
     gene            15784..16185
                     /locus_tag="O2692_000338"
     CDS             15784..16185
                     /locus_tag="O2692_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVL family protein"
                     /protein_id="HCZ4297987.1"
                     /translation="MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDE
                     LLCGRKDSKSKDEVEYMKSQIKDEEKEREKIRKKAILNLYQRNVRAEYEEERKRRLRP
                     WLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            16185..16442
                     /locus_tag="O2692_000339"
     CDS             16185..16442
                     /locus_tag="O2692_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HCZ4297988.1"
                     /translation="MSIISNRKVDMNETQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWEG"
     gene            16445..16645
                     /locus_tag="O2692_000340"
     CDS             16445..16645
                     /locus_tag="O2692_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297989.1"
                     /translation="MATQKQVDYVMSLQEQLELEDCEKYTDEQVKAMSHKEVSNVIEN
                     YKTSIRNEELYYECMSFGLPNC"
     gene            16660..16902
                     /locus_tag="O2692_000341"
     CDS             16660..16902
                     /locus_tag="O2692_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297990.1"
                     /translation="MTDSARKERLNQFFGSKRYLYQDNERVAHTHVVNGTYYFHGHIV
                     PGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            16917..17165
                     /locus_tag="O2692_000342"
     CDS             16917..17165
                     /locus_tag="O2692_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCZ4297991.1"
                     /translation="MNNREQIEQSIISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEDQEND"
     gene            17158..17694
                     /locus_tag="O2692_000343"
     CDS             17158..17694
                     /locus_tag="O2692_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HCZ4297992.1"
                     /translation="MTNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWF
                     NTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKNSEF
                     HNMFNFKDKEFAQDAVVSTPQIIFKEFYPDQLAIVIAIDIAFNLYSIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            17731..17937
                     /locus_tag="O2692_000344"
     CDS             17731..17937
                     /locus_tag="O2692_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCZ4297993.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAERKY
                     EAQVKRDAVIKVGQLFENIRECGK"
     gene            17934..18137
                     /locus_tag="O2692_000345"
     CDS             17934..18137
                     /locus_tag="O2692_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297994.1"
                     /translation="MTEVKIKTISDRVYYTTTDLASDDYINLVMNLVIEDFLPVKDVF
                     NNEVWVKRDEIESFTFIKEANDD"
     gene            18130..18366
                     /locus_tag="O2692_000346"
     CDS             18130..18366
                     /locus_tag="O2692_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297995.1"
                     /translation="MINIPKMKFPKKYTEIIKKYKNKAPEEKAKIEDDFIKEIKDKDS
                     EFYSPTMANMNEYELRAMLRMMPSLIDTGDDNDD"
     gene            18359..18745
                     /locus_tag="O2692_000347"
     CDS             18359..18745
                     /locus_tag="O2692_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4297996.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGIFALLLVITLPIYTVASYQHK
                     ELHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKVE
                     VKTIGYRIHFLNLYPVLYEVKKVDKQ"
     gene            18742..18894
                     /locus_tag="O2692_000348"
     CDS             18742..18894
                     /locus_tag="O2692_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ4297997.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYEKI
                     RAEVSW"
     gene            18962..19162
                     /locus_tag="O2692_000349"
     CDS             18962..19162
                     /locus_tag="O2692_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCZ4297998.1"
                     /translation="MWIVISIVLAIFLLILLSSISHKMKTIEALEYMNSYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKS"
     gene            19450..21897
                     /locus_tag="O2692_000350"
     CDS             19450..21897
                     /locus_tag="O2692_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HCZ4297999.1"
                     /translation="MLDKVTQIETIKYDRDVSYSYAASRLSTYWTNHNMAWSDFMQKL
                     AQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYA
                     AQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVG
                     MDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLPLLDPDKILNEYVDWTDTLEWPTSS
                     KEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAISTFIPDLYEKHSTNRYTYHEGS
                     TAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLVRIHLYGAQDEDAKTDTPVNRLPSY
                     KAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVNSDDAWSETLEITSKGTFKASIPNI
                     EIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDI
                     HHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTK
                     KALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEA
                     LQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKV
                     DFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPELE
                     EEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDDLTIFERRRFYQGDVDMLPTGNVDY
                     VERNKVCALEVFVECFGKDKGDSRGSMEIRKISNILRQLDNWSVYDGNKSGKIRFGKD
                     YGVQIAYVRDESLEDLI"
     gene            complement(22006..22104)
                     /locus_tag="O2692_000351"
     CDS             complement(22006..22104)
                     /locus_tag="O2692_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298000.1"
                     /translation="MNLMQHWKQTGLNLTARRERGVVSFVASMLHH"
     gene            22238..22528
                     /locus_tag="O2692_000352"
     CDS             22238..22528
                     /locus_tag="O2692_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCZ4298001.1"
                     /translation="MKESTLEKYLVKEISKLNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHKVYVLWNKEQVNTFIRMVGGTFGD"
     gene            22509..23876
                     /locus_tag="O2692_000353"
     CDS             22509..23876
                     /locus_tag="O2692_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCZ4298002.1"
                     /translation="MEHLAIDFKPHSYQKYAIDKVIDNEKYGLFLDMGLGKTVSTLTA
                     FSELQLLDTKKMLVIAPKQVAKDTWVDEVDKWNHLNHLKVSLVLGTPKERNDALNTEA
                     DIYVTNKENTKWLCDQYKKEWPFDMVVIDELSTFKSPKSQRFKSIKKKLPLINRFIGL
                     TGTPSPNSLQDLWAQVYLIDRGERLESSFSRYRERYFKPTHQVSEHIFKWELRDGSEE
                     KIYKQIEDICLSMKAKDYLDMPDRVDTKQTVVLSEKERKVYEELEKNYILESEEEGTV
                     VAQNGASLSQKLLQLSNGAVYTDEEDVRLIHDKKLDKLEEIIEESQGQPILLFYNFKH
                     DKERILQRFKEATTLDDSNYKERWNSGDIKLLIAHPASAGHGLNLQQGGHIIVWFGLT
                     WSLELYQQANARLYRQGQNHTTIIHHIMTDNTIDQRVYKALQNKELTQEELMKAIKAR
                     IAKHK"
     gene            23889..24326
                     /locus_tag="O2692_000354"
     CDS             23889..24326
                     /locus_tag="O2692_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ4298003.1"
                     /translation="MGNTIYDIKPGTFKYIESEIYNLNENKKEIKRLRLEILNPTKEQ
                     DSNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKEKKLKMEQIGHECHMHRNTVTTIRKNFVKAVAYHAGIK"
     gene            24483..24797
                     /locus_tag="O2692_000355"
     CDS             24483..24797
                     /locus_tag="O2692_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCZ4298004.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNHLCQMC
                     LREDIVTDANIVHHIIYVDEDFNKALELDNLMSVCYGCHNKIHANGNDKSNLKKIRVL
                     KI"
     gene            24924..25229
                     /locus_tag="O2692_000356"
     CDS             24924..25229
                     /locus_tag="O2692_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HCZ4298005.1"
                     /translation="MKLTKKQLKEYIEDYKKSDDILINLYIETYEFYCRLRDELKNSD
                     LMIEHTNKAGASNIVKNPLSIELTKTVQTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            25219..26910
                     /locus_tag="O2692_000357"
     CDS             25219..26910
                     /locus_tag="O2692_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCZ4298006.1"
                     /translation="MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVKKECERHLRYL
                     ENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLDKNFRTLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSINLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNEIVSLEELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLSHSWI
                     PKHKVEYSNEKIPYREWEEDGLLTVQDKPYIDYQDVLNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDKGEGNIEFISIKDIMR"
     gene            26915..28153
                     /locus_tag="O2692_000358"
     CDS             26915..28153
                     /locus_tag="O2692_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCZ4298007.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSASKLYDFSPWKNKSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
     gene            28137..28910
                     /locus_tag="O2692_000359"
     CDS             28137..28910
                     /locus_tag="O2692_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCZ4298008.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQ
                     MLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY"
     gene            28922..30085
                     /locus_tag="O2692_000360"
     CDS             28922..30085
                     /locus_tag="O2692_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCZ4298009.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGPLPS"
     gene            30154..30432
                     /locus_tag="O2692_000361"
     CDS             30154..30432
                     /locus_tag="O2692_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCZ4298010.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            30444..30776
                     /locus_tag="O2692_000362"
     CDS             30444..30776
                     /locus_tag="O2692_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298011.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIVRSSKTEYLPQTNHLVKIDSALYSDKLFNIVEIRIDTPDIGY
                     NTVVLSEK"
     gene            30773..31174
                     /locus_tag="O2692_000363"
     CDS             30773..31174
                     /locus_tag="O2692_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298012.1"
                     /translation="MSVEIKGIPEVLNKLESVYGKQAMQAKSDKALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAASERKYREIIKKELAR"
     gene            31175..31570
                     /locus_tag="O2692_000364"
     CDS             31175..31570
                     /locus_tag="O2692_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCZ4298013.1"
                     /translation="MNILNTIKGILLSDAELKTHINSRIYYYKVTENAETSKPFVVIT
                     PVYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            31605..32246
                     /locus_tag="O2692_000365"
     CDS             31605..32246
                     /locus_tag="O2692_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCZ4298014.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSDDLNTVIGRTKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            32338..32793
                     /locus_tag="O2692_000366"
     CDS             32338..32793
                     /locus_tag="O2692_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCZ4298015.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG"
     gene            32851..33201
                     /locus_tag="O2692_000367"
     CDS             32851..33201
                     /locus_tag="O2692_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298016.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALQDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            33243..33401
                     /locus_tag="O2692_000368"
     CDS             33243..33401
                     /locus_tag="O2692_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298017.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            33415..39613
                     /locus_tag="O2692_000369"
                     /pseudo
     CDS             33415..39613
                     /locus_tag="O2692_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            39613..40437
                     /locus_tag="O2692_000370"
     CDS             39613..40437
                     /locus_tag="O2692_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCZ4298018.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            40446..42029
                     /locus_tag="O2692_000371"
     CDS             40446..42029
                     /locus_tag="O2692_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCZ4298019.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFDCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            42029..42319
                     /locus_tag="O2692_000372"
     CDS             42029..42319
                     /locus_tag="O2692_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298020.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDKLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            42335..44245
                     /locus_tag="O2692_000373"
     CDS             42335..44245
                     /locus_tag="O2692_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298021.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTMQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            44245..45711
                     /locus_tag="O2692_000374"
     CDS             44245..45711
                     /locus_tag="O2692_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCZ4298022.1"
                     /translation="MSNLEKSVAINLENTAHYENISNLDITFRTGESDSSVLLFNIIK
                     NNQPLLLSEENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDGSYQAQV
                     SVAELGNSDVVVVERTITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRAKAMDEA
                     IKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSRISDLENKAQAYSRTFDEQ
                     KRYMDEKHEAFKQSVNSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGD
                     STQFIYVSQAINYPRDVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEAGSWSEW
                     SELAINDYNTPFETVQSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGVGSTLYL
                     NESLDQFILLIFYGTFPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYEFGLTKS
                     SRTSLTISNDVYFDLGSQRGSGANANRGTINKIIGVRK"
     gene            45711..46100
                     /locus_tag="O2692_000375"
     CDS             45711..46100
                     /locus_tag="O2692_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HCZ4298023.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            46093..46257
                     /locus_tag="O2692_000376"
     CDS             46093..46257
                     /locus_tag="O2692_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCZ4298024.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYSKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENLES"
     gene            46303..46602
                     /locus_tag="O2692_000377"
     CDS             46303..46602
                     /locus_tag="O2692_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCZ4298025.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRTQEKIYDK
                     LDRNFEELRRDKEEDEKNKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTIFGI"
     gene            46738..47040
                     /locus_tag="O2692_000378"
     CDS             46738..47040
                     /locus_tag="O2692_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCZ4298026.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDEESVSSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAESKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            47051..48505
                     /locus_tag="O2692_000379"
     CDS             47051..48505
                     /locus_tag="O2692_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCZ4298027.1"
                     /translation="MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMLATGE
                     RLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNK
                     AKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSE
                     LGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGVVASEVKAPVKNEKNPPVPAGYT
                     LDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDIAGNRISSFGKFSAV"
BASE COUNT        18336 a         6745 c         9335 g        14340 t
ORIGIN      
        1 ttatttttaa agaggataaa gtatttaata atattaacaa aatcatttaa taaatagtta
       61 aatatatatt ctctgttttt gtgatattat tcacatgtcg atacatatca acaaatatca
      121 atcatacgaa agaaggttat aacaatgaaa aataaaaaac gtgttttaat agcgtcatca
      181 ttatcatgtg caattttatt gttatcagca gcaacgactc aagcaaattc agctcataaa
      241 gactctcaag accaaaataa gaaagaacat gttgataagt ctcaacaaaa agacaaacgt
      301 aatgttacta ataaagataa aaattcaaca gcaccggatg atattgggaa aaacggtaaa
      361 atcacaaaac gaactgaaac agtatatgat gagaaaacaa atatactcca aaatttacaa
      421 ttcgacttta tcgatgatcc aacttatgac aagaatgtat tacttgttaa aaaacaaggc
      481 tcaattcatt caaatttaaa gtttgaatct cataaagaag aaaaaaattc aaattggtta
      541 aagtatccaa gtgagtacca tgtagatttt caagtaaaaa gaaatcgtaa aactgaaata
      601 ttagaccaat tgccgaaaaa taaaatttca actgcaaaag tagacagtac attttcatat
      661 agctcaggtg gtaaattcga ttcaacaaaa ggtattggac gaacttcatc aaatagctac
      721 tccaaaacga ttagttataa tcagcaaaat tatgacacaa ttgccagcgg taaaaataat
      781 aactggcatg tacactggtc agttattgcg aatgacttga agtatggtgg agaagtgaaa
      841 aatagaaatg atgaattatt attctataga aatacgagaa ttgctactgt agaaaaccct
      901 gaactaagct ttgcttcaaa atatagatac ccagcattag taagaagtgg ctttaatcca
      961 gaatttttaa cttatttatc taatgaaaag tcaaatgaga aaacgcaatt tgaagtaaca
     1021 tacacacgaa atcaagatat tttgaaaaac agacctggaa tacattatgc acctccaatt
     1081 ttagaaaaaa ataaagatgg tcaaagatta attgtcactt atgaagttga ttggaaaaat
     1141 aaaacagtta aagtcgttga taaatattct gatgacaata aaccttataa agaaggataa
     1201 tattgaaagg gcggattact aatgattaaa caactatgta aaaatatcac aatttgtacg
     1261 ttagcactat cgactacttt cactgtatta ccagctactt catttgcaaa gattaattct
     1321 gaaatcaaac aagtttctga gaagaatctt gatggtgata ctaaaatgta tacacgtaca
     1381 gctacaacaa gtgatagtca aaaaaatatt actcaaagct tacaatttaa tttcttaact
     1441 gaacctaatt atgataaaga aacagtattt attaaagcaa aaggtacaat tggtagtggt
     1501 ttgagaattt tagacccaaa tggttattgg aatagtacat taagatggcc tggatcttat
     1561 tcagtttcaa ttcaaaatgt tgatgacaac aacaatacaa atgtgactga ctttgcacca
     1621 aaaaatcagg atgaatcaag agaagttaaa tatacgtatg gttataaaac aggtggagat
     1681 ttttcgatta atcgtggagg cttaactgga aatattacaa aagagagtaa ttattcagag
     1741 acgattagtt atcaacaacc atcatatcgt acattacttg atcaatctac gtcacataaa
     1801 ggtgtaggtt ggaaagtaga agcacatttg ataaataata tgggacatga ccatacgaga
     1861 caattaacta atgatagtga taatagaact aaaagtgaaa ttttttcttt aacacgaaat
     1921 ggaaatttat gggcgaaaga taatttcaca cctaaagaca aaatgcctgt aactgtgtct
     1981 gaagggttta atccagaatt tttagctgtt atgtcacatg ataaaaaaga caaaggtaaa
     2041 tcacaatttg ttgttcatta taaaagatca atggatgagt ttaaaataga ttggaatcgc
     2101 catggtttct ggggctattg gtctggtgaa aaccatgtag ataaaaaaga agaaaaatta
     2161 tcagcattat atgaagttga ttggaagaca cataatgtga agtttgtaaa agtacttaat
     2221 gataatgaaa agaaataagt aataaaagtt gcctgctaca tagaatgtag taggtaactt
     2281 ttatttatat ttgaatagat agatttatta tgacgtgcag tgtatgaatc ttgtttgagt
     2341 gtagagtaaa gacttgtatt aatgaaaaat taaagttgtt aagatgattt tattaaaatg
     2401 taagtcaatt caaattttaa caatataact cgcttcgtcc ttttagaacg aagcaagtta
     2461 ttagttagtt gagcactatt tactataggc tttgattggg taatgatctg aaaaatcatt
     2521 gtaaacgtag taatatggga acgcatatac atcccatggc ttaggttttt cagtcacaac
     2581 ttcattgact aattgttttg gttgtttatg atctttatct gtaaatatat agtctaaatg
     2641 ttctggttta ccattagggt aattatattt cgcaattgaa tttgattgag ggtcccatgt
     2701 gctattatga cctgcatata gaacatcatt tacattcaag tttttaagca tatctttgaa
     2761 ctctggagtg cctttattga cattaaggtc gccacctata tataccgttt catctttagg
     2821 gatatttttc tttttaacaa agtcactgat ttctttcatt tgttcagctc taatttttcg
     2881 atcatgtcca gcaccacaac gtgaatcttc agattgtgta tgtgtaccga taacgtgaac
     2941 gttcttacca tttttctcta tttttgtata aacaaagcct ttgttgctat cattatcgaa
     3001 tccacaaccg cttttgaaaa catgctggat tttttcttta ataggatatt tacttacaat
     3061 cgctacgcca ccatcttctg caacagttga tgagtagcta ccttcagttt tgtcccaccc
     3121 tgattgagaa cggccgagta caggtgtttg ataaggatat tcttttttca cattacttaa
     3181 taatttgtct gatgcaccat tatcaaatgc ttcattgaat attacgacat cattattttt
     3241 aatataagaa gattgtccga ttaaatcagc gcgtttatat tgcccccagt ttggatacat
     3301 agaaaccttg taacaacagt atttattggg tttggagtcc ctaatgggtc cctaaattac
     3361 atactttcta aaattttagt tgtttttttg tcctcttcat taaatttttc ttctaacaaa
     3421 tgagaataca cggatgtagt tattgctata tttttatgac ctaatctttt agaaatgtaa
     3481 tgtatagata caccttttgc tagtaaataa gaacaatgag tgtgtcttaa tgcgtgcgat
     3541 gtaataattg atatattatt gactctacag gctgatttca aagcattatt gatagcatga
     3601 aggttaatta tagatccggc ttctttgaaa atgtaaccat catagctaat tgcaaatgta
     3661 cttatgacgt ccataatgtg tttcatatca gatttagcga tactgatata tctaggggaa
     3721 gtatcggttt ttcgctcgtc aataaatata gtgtttttca cttggttgat atgctcaatc
     3781 tttatatttc ttgcaccact gacacgacaa cccgtacaaa tcattatgaa tagcgctaat
     3841 gatgaacgag ttctcttctt tctgacgtga tcttttagta tttcatattc agttaccgag
     3901 atgaattttt cttgttctga cttcgtaggt tttccggctt tataattaac tttataagcg
     3961 ggatttttaa aaataagccc atcatataat gcgtcatcta aagctgaccg aatagcaccg
     4021 tttgtttttc ttatagtttc ttttgcgtgt tcttttgaat aatcgtttat gaatttctga
     4081 taaacttgtc tatttatctt tgataactcc attttaccta ttttatgttt ttgtatatgt
     4141 tgtaatgcat ttctataatg acggtaggta ttttctttaa caacaggttg tttatacgtt
     4201 ttaatccaat tttcgaagta ttcttcaaga gttatatagt tatctatatt aaaaccactt
     4261 cttaactcat ttaacttgtc tagtccagca gaattagctt cacgctttgt tctaaaacct
     4321 ttcttacggt atctttttcc ttcatactta aattcatatt gccatttttt accatcgtaa
     4381 caacgtgttt tcatgcgttc cctcctcaaa attggcaaaa aataataagg gtaggcgggc
     4441 tacccatgaa aattgtataa aaaaagacgc ctgtataaaa tacagacgcc acttatagtt
     4501 ataagattac atggttaatt accaaaaatg gtaacgaata tatacgtgtt ttaaaggata
     4561 aacctttaat atattaaaat tatatcatct tatatcagga atctgcaata tattattatt
     4621 aattctattt atcagtaaca taatatccga agaatctatt actggatttt taattttttg
     4681 gggtaaaact tttcttatgc gaaacttact aatcggctgg aaagaattta tgcaagcgta
     4741 actattacct tttaattttt ttaccttatc aattgctgat actatgttat taatgtttct
     4801 gtcaatttta tttaatttat tttcaatttc taaactatca gatataaatt cactaaaata
     4861 atctttagtg atgaattctg tgttgttttt ttggtatttt ttatcgaaaa cttcttttaa
     4921 tatagctgaa ttattttgcg cgctaattaa atttaaaaac aatcttaaat aatactccca
     4981 tttcaaatca aaattcatct ttaaatactt tttgttttct ttagaagata agggaataac
     5041 atttactata tcttccgtat tagaatcatt tttattcatc actattgcaa agtgtgaatt
     5101 agagaattct ttattaacgt ttataccgaa atctacaaaa actatttctc cttgtttaaa
     5161 ctttggataa aaacctttat ggtttttttc accttcaaat ctcttgagta aatagtgaat
     5221 atctgaatct aattttttaa attttggatt tccagaagtt tttaatttat taatgcgttt
     5281 ttctatatta tgcgtcatca tttctccttt attctcgctc acactctcac caccattcaa
     5341 cgtctacact tgtagacgtt ttttgattag taaaatcata atgaatcttc tttggttaac
     5401 ttatcgccat ctattttttg tgaaataaat tccaagtatt tacgcgcatt atgtgacgat
     5461 aaatctttag gtaactcata agtgaatggt tgattaccac tagttaaaac ttcgtatatt
     5521 acagtttctc tttttatttt gcaattagtt attttcatta taaacttcct ttcaaacact
     5581 gctgaaatag acgtcttttt taaataagca taattaatac ttcaattctt taatccacat
     5641 atatttaaaa gtgaggtagt aggtaataaa tataagactt aaagttaaga ttgctttttt
     5701 catgtttcat aattaaaacc tctgtaaatt taaggttagt attatgaaat aatggattgg
     5761 tttattcttt agtactaact tcgtagtaaa ttatatagtt cgctaaattg tatttatcta
     5821 ctatattttt ggaataaaca atttcctttt ctttcttcag taaattataa aaatctacat
     5881 cattttcgtt agctgattct attttggtga tatcagattg tctaacgatt cttttagatc
     5941 tgtcaaggta tataaatttc cctgatttag aattagtctt tttattcaca ccgacgtaaa
     6001 ttgaaagtaa tatacttttt ccaaaaacag catcactgtg attatggttt tcatcttcta
     6061 taactaagaa tacatgcttt tttgtaaaaa tttttttgat catcgttatt attcctttat
     6121 taaatttatt aagtcttctt catttaaaaa ttgaattttt gcaccatttc caacatattc
     6181 tcgagctttt cgttgttttg aaactagtcc gttcacatct ttatatttat catcttgaac
     6241 accttcgact aaaatatctg tttttgcagt tacgtcactt ctgatataag ctcctttctt
     6301 tctagttaat atcattaaat cttgtttttc agtgtcaaaa ttacctgtaa aaacaacatt
     6361 tttatctttt aaaataggga ttacactttc cacttctatt ttattaatct cagatatttt
     6421 catatgaatt ttttgaaatc ctgaatcgaa aagtttagtt ggagagttag aatatttgct
     6481 aaatctaatg tattgcttag gcatataatg tattaatttt aatacactat aatgctgatt
     6541 gtttttagcg agtgatatca acatcttcga taaagctagc acgtcaaatt tagcagaatg
     6601 taatttttct ttatcgatat catataagct acacaaattt tctaatttaa aactagagat
     6661 tgcgtggaag cttctaaaga tatttatact atcgacatac atgaagtttg gaacaggtaa
     6721 gtcataataa ttattagtat tttttaatac tgaaatatca aaaagtgcat tatgagcaat
     6781 aattaaatgt gattctttta aaagatagag aatttcttgg taaatatctg gatatttagg
     6841 tgcttttaat atgacatctt caggtatttt atgtatttta gcgtttttca agttatatct
     6901 attattagga ggattaatat aagatgaata aacttttact attgataaat ccttaattaa
     6961 agatacagca acttcgcaag ggctgttcat atgttcattc atagtttcaa agtctaagac
     7021 tgcaatatca tattttttca tttgcaagtg ctccttttat aaaataactt ttccaattaa
     7081 cctcacactt tcatttctat aaaagtgtag atcgtcgtaa tctttattta gtgaaactag
     7141 agtcaatcta tcatcttcaa caaagacttt cttaacgtac gcttcttctt caatgatgaa
     7201 tataccaatt tgtccattct ttatattgtg agttttctcc acaaatatga tttcgccatc
     7261 tttaaacata ggttccatag aatcaccatt tacttttaac gctaaatcgt gtgtggggat
     7321 aggtccttta accatttcag taaatagcgt ttcatcgtgt aaacgttctc ctacaccagc
     7381 agagacgcaa ccattgacgt taactggagt tttctcctgt ttatatgaat taatatctac
     7441 aacgttatct cctttagaat tctgttcttc caattgttca tttgcatagt taagtacgtt
     7501 ttcttggcgg ggaggtgtga gtttgttgta tatggaagtg atgtcgttat cgtctttgta
     7561 tgtagtatct atgtcgcttt taccaacctc gaaaacatca gctatccttt gtataacgcc
     7621 gtgagagggg ttggaacgta aatttaaata atcgcttaaa gtagatggtt ttatgttaat
     7681 gagttcagca agtttctttt gagacatatt tgaatcgttg agaaattttc taatgttttt
     7741 ggctataata atatttcttt ctttgttcat attacttacc tccttttttc ttattatacg
     7801 aaattttcat atcatagtaa agttttttac gaaaaaaacg tatttaatgt tgacaatacg
     7861 aaaatttcgt attatattag gtttacgaaa ggcggtgaca acatgaaaac attaaaagag
     7921 ttgaggactg attacggatt gactcaaaaa gagttaggag atttatttaa ggtctcatca
     7981 cgtacaattc aaaatatgga aaaagactct acaaacatta aagatagttt actttctaag
     8041 tatatgagtg cttttaatgt taaatatgat gatatttttt taggtaatga atacgaaaat
     8101 ttcgtattta cgaatgataa aaagaaatca attattttag catttaaaga aaaacaaaca
     8161 tcttaatagg aggaataaca aatgaacatt caagaagcaa cgaagctagc gatggagaaa
     8221 ggaataagta taaggagaga gaatcaagat gtgtatggga tattaccaac taatttgcag
     8281 cgttatcaat gcctagtcgt atctagacac tataagaaaa aaagacaaac cgccgccgga
     8341 aggtggcagc ctagcgcaga cgatttaata gcagatgatt ggattttaga ttattaattt
     8401 tttcaaatct ctaattaaat ccataagtgt tttgtaatct tttttggatt ctgattctga
     8461 gtaggcgata ccttctcgag aaagagccat ctcaagaaaa ccgccatctt cagcagaagc
     8521 aattacaaaa tctctatgct ttaattcaag aactgcatcg atatagtctt caaaattaaa
     8581 atctaaaaag aaagcgttaa atgaggattc atcactaccg aaataagatg cagaacgttt
     8641 agacatacct tcgtcaattc tatcaaggta aattgaataa agttgtaaaa ggacaaattt
     8701 agcttcatta gtcataagtc attcacctcc ttaataggag tatagcagaa aggagcataa
     8761 acaatatgca agcattacaa acaaattcga acatcggaga aatgttcaat attcaagaaa
     8821 aagaaaatgg agaaatcgca atcagcggtc gagaacttca tcaagcatta gaagttaaga
     8881 cagcatataa agattggttt ccaagaatgc ttaaatacgg atttgaagaa aatacagatt
     8941 acacagctat cgctcaaaaa agagcaacag ctcaaggcaa tatgacacac tatattgacc
     9001 acgcactcac actagacact gcaaaagaaa tcgcaatgat tcaacgtagt gaacctggta
     9061 aacgtgcaag acaatacttc atccaagttg aaaaagcatg gaacagccca gaaatgatta
     9121 tgcaacgtgc tttaaaaatt gctaacaaca caatcaatca attagaaaca aagattgaac
     9181 gtgataaacc aaaaattgta tttgcagatg cagtagctac tactaagaca tcaattttag
     9241 ttggagagtt agcaaagatc attaaacaaa acggtgtaaa catcgggcaa cgcagattgt
     9301 ttgagtggtt acgtcaaaac ggattcctta ttaaacgcaa gggtgtggat tataacatgc
     9361 ctacacagta ttcaatggaa cgtgagttat tcgaaattaa agaaacatca atcacacatt
     9421 cggacggtca cacatcaatt agtaagacgc caaaagtaac aggcaaagga caacaatact
     9481 ttgttaataa gtttttagga gaaaaacaaa catcttaaaa ggaggaataa caaatgaacg
     9541 aagaaaaatt aaagatgatt ctcttacttc ttgaagatat accaagagac gaatggaatc
     9601 gattagtaaa tgaagtaaat aatcaataca gttaccaagc tgacaaggta ggacttgcca
     9661 gtgataactg tcaacaaata gcaaataact ataagcacta tggattttaa gatggataag
     9721 gactatcgtt attcttttca cctttaacgt taatgtcgaa atacaaaaca tgatctaaac
     9781 tgacaactct atcttttgga taaatgatgt gtagagtttt gctttcagca tctttcatat
     9841 gttcttgtac tttaataact tcaccattaa ttaaatgtaa atctaaagaa gtaacgattt
     9901 ctttatctaa atattgagtg agcaatctct tatgcatact tatcacctcc tttcactagg
     9961 agataacaac attatacacg aaaggaaaga tagaaatgcc acacatttta aacgtaacag
    10021 ttccaatacc tgaaacacat gtacttatca caaaagatga atatgatgag ctaattggtt
    10081 attcattaga ccctgtatgg aacatgagtg acttaaagaa gaaattaaaa attgcatctg
    10141 atgagactat caaggacaga ttactatttc atcctagatt tgaaaaagaa ctaagagcgc
    10201 aaggaattgt gcattaccca gatgagagtt ttaatcgctg gagatttaac gcaagaaaga
    10261 tgaataaatt cgtcgatgag catttcaatg aaatatataa ggagagaata aaatgagcga
    10321 cacatataaa agctacctat tagcagtgct atgtttcaca gtcttagcga ttgtgcttat
    10381 gccattgctg tacttcacta cagcgtggtc aattgcagga ttcgcaagca ttggaacatt
    10441 catatactac aaagaatact tttatgaaga ataaaaaaac tgctacttgc gtcaacaagt
    10501 aacagtgaca aacatttatc aaaatataca acttaattaa atcaaaatat acggaggtag
    10561 tcaactatgg ctgaaaatat taaaactgaa caacattatt acactaaaga tttctcagga
    10621 tacagaaatg aagaagataa ctttgtagca aatcaagaat tgacagtaac aatcacattg
    10681 aacgagtaca gaaaacttat tgaaataaag gctgttaaag ataaagaaga agatacttac
    10741 agaggtaagt attttgcgga agaaagaaaa aacgaaaaat tggaaaaaga aaatataaaa
    10801 ctaaaaaaca aaatttatga attacaaaac gaagaagata acgaggagga cgaagaaaac
    10861 gaggaggaag tcaacaatga ctaaaaaata taaagacatg actcaggaag aagtaaaaga
    10921 cttattatct gaaaaaagcg gagaattgta tgaattagcg aaagaaatta agggagaaag
    10981 taaatttgat attttgcttt tctcatcaat aggagttatc gacggagatt atttagcagg
    11041 ttcaagttct gtgattggtc atactttcga tcttgcttcc ttattggata gcactaagag
    11101 ttataaagac attgtcaatg ttctccaaat gtgtaaatca caaaaatttc acggtattga
    11161 tgataacaag gagggctaaa acaatgtatt acaaatttgg tgagataaaa aataaaatta
    11221 tcagctttaa cgggtttgaa tttaaagtgt ctgcgatgaa aaaacatgac ggtatcagta
    11281 tacaaatcaa ggatatgaat aatattccac ttaaatcatt tcatgttgta gatttaagcg
    11341 aactatattt tgcgacggat gcaatgcgtg acgttataaa cgaatggatt gaagagaaca
    11401 cagatgaaca ggacagacta attaacttag tcatgaaatg gtaggaggat ttaatcaatg
    11461 gcaatattag aagatatttt tgaagaatta aaactattaa ataacaattt acgtgtgtta
    11521 aacactgaac tatcaacagt agattcatca attgtacaag agaaagttaa agaagcacca
    11581 atgccaaaag aagaaacagc tcaactggaa tcaattgaag aagttaagga aacttctgct
    11641 gatttaacta aagattatgt tttatcagta ggaaaagagt tccttaaaaa agcagatact
    11701 tctgataaga aagaatttag aaataaactt aacgaacttg gtgcggataa gctatctact
    11761 atcaaagaag agcattatga aaaaattgtt gattttatga atgcgagaat aaatgcatga
    11821 agctagatca ctcaaataga gctcatgcaa agcttagtgc aagtggagca aaacaatggc
    11881 taaactgccc accgagtatt aaggcaagtg aaggtattgc agataaaagt acagtttttg
    11941 ccgaagaagg tacattcgcc catgagttaa gtgagttata tttcagcctt aaatatgaag
    12001 gcctaacaca gtttgagttt aataaggctt ttcaaaatta taagagaaat caatattaca
    12061 gcgaagaatt gcgtgaatat gtggaagagt atgtagctaa tgtagaagaa aaatataacg
    12121 aagctttgag tagggataat gatgtaatag ctttatttga aacaaaatta gatttaggta
    12181 aatacgtccc tgaatctttt ggaactggtg atgtcattat attttcaggt ggtgtacttg
    12241 aaattattga ccttaaatat ggtaaaggca ttgaagtttc agctatagat aatcctcaac
    12301 ttagattata tggcttgggc gcatatgaac tgcttagttt aatgtatgac attcatacaa
    12361 ttcgcatgac tatcatacaa cctcgaatag ataacttttc tactgaagag ttaccaatat
    12421 caagattact tcaatgggga gccgattttg ttaaaccatt agccagactt gcttataacg
    12481 gtggaggaga gtttaaagca ggtagtcatt gtagattctg taagataaat cattcatgta
    12541 gaacacgtgc agaatacatg caaaatgtgc ctcaaaagcc accacatttg ttaagtgatg
    12601 aagagattgc agaactttta tataaactgc ctgatatcaa aaaatgggct gatgaagtag
    12661 aacaatatgc actaaatcaa gcgaaagaaa atgataaaaa ctatcctggt tggaagcttg
    12721 tagaaggtcg ttcacgaaga atgataactg atacaaaagc aatgcttgaa aagttagttg
    12781 aagcgggtta taaacctgaa gatattacag aaaccaagtt acttagcatt acgaatttag
    12841 aaaaattaat tggtaaaaaa gcattttcta aaattacaga gggttttata gaaaagccgc
    12901 aaggtaaatt aacacttgct accgagtcgg ataaacgacc agctataaag caatctgctg
    12961 aagatgattt tgacaaacta taaaaattta aaaggacggt atataaacat gaaagcaaaa
    13021 gtattaaata aaactaaagt gattacagga aaagtaagag catcatatgc acatattttt
    13081 gaacctcaca gtatgcaaga agggcaagaa tcaaagtatt caatcagttt aatcattcct
    13141 aaatcagata caagtacgat aaaagccatt gaacaagcta tagaagctgc taaagaagaa
    13201 ggaaaagtta gtaagtttgg aggcaaagtt cctgcaaatc tgaaacttcc attacgtgat
    13261 ggagatactg aaagagaaga tgatgtcaat tatcaagacg cttattttat taacgcatca
    13321 agcaaacaag cacctggtat tattgaccaa aacaaaatta gattaacgga ttctggaact
    13381 gttgtaagtg gtgattatat tagagcttca attaatctat ttcctttcaa cacaaatggt
    13441 aataagggta tcgcagttgg attgaacaac attcaacttg tagaaaaagg cgaacctctt
    13501 ggcggtgcaa gtgcagcaga agatgatttc gatgaattag acactgatga tgaggatttc
    13561 ttataagtca ataggtgggg ttttagcccc actttaattt taaagaaatt gaggtgtcaa
    13621 gaatttgaga tttatgaata tagatattga aacatatagc agtaatgata tttcgaaatg
    13681 tggtgcctat aaatacacag aagctgaaga tttcgaaatt ttaattatag cttattcaat
    13741 agatggtgga gcgattagtg cgattgacat gactaaagta gataatgagc ctttccacgc
    13801 tgattttgag acgtttaaaa ttgctctttt tgatcctgct gtaaaaaagt atgcattcaa
    13861 tgctaatttc gaaagaactt gtcttgctaa acattttaat aaacagatgc cacctgaaga
    13921 atggatttgc acaatggtta attcaatgcg tattggctta cctgcttcgc ttgataaagt
    13981 tggagaagtt ttaagactac aaaaccaaaa agataaagca ggtaaaaatt taattcgtta
    14041 tttctctata ccttgtaaac caacaaaagt taatggagga agaacaagaa acttgcctga
    14101 acatgatctt gaaaaatggc aacaatttat agattactgt attcgagatg tagaagtaga
    14161 aatgacgatt gctcataaaa ttaaagactt tccagtaact gcaattgaac aagcatattg
    14221 ggtttttgac caacatataa acgacagagg tattaagctt tctaaatcat tgatgttagg
    14281 agctaatgtg ctcgataagc agagtaaaga agaattgctt aatcaagcta aacatataac
    14341 aggtttagaa aatcctaata gtcctacaca attattggct tggttaaagg atgaccaagg
    14401 attagatata cctaatttac aaaagaaaac ggttcaggag tacttaaaag aagcaacagg
    14461 aaaagctaaa aaaatgctag aaattagatt gcaaatgtct aaaaccagtg tgaaaaaata
    14521 caacaaaatg catgacatga tgtgcagtga tgaacgggta agaggtctgt ttcaatttta
    14581 cggtgccggt actggaagat gggcaggtag aggtgtacaa cttcagaatt taacaaagca
    14641 ttatatttca gatactgaat tagaaatagc aagagatctt attaaagaac aacgttttga
    14701 cgatttagat ttattactca atgttcatcc tcaagactta ttaagtcaat tagttaggac
    14761 gacatttact gctgaagaag gtaatgaact agcagtaagt gatttttctg caatagaggc
    14821 aagagtcata gcatggtatg caaaagaaca atggcgttta gatgtgttca acacacacgg
    14881 aaagatatat gaagcatcgg cttctcaaat gtttaatgtc ccggtagaaa gcataactaa
    14941 aggcgaccct ctcagacaaa agggaaaagt gtccgaatta gctttaggtt atcaaggtgg
    15001 cgctggagct ttaaaagcga tgggcgcatt ggaaatgggc attgaagaaa atgaattaca
    15061 aggtttagtt gatagttggc gtaacgcaaa tcctaacata gttaattttt ggaaggcttg
    15121 ccaagaggct gcaattaata ctgtgaaatc ccgaaagacg catcatacgc atggagttag
    15181 attttatatg aaaaaaggtt ttctaatgat tgaactgcct agtggaagag ctttagctta
    15241 tccaaaagct tcagttggtg aaaatagttg gggtagtcaa gttgttgaat ttatgggctt
    15301 agatcttaac cgtaaatggt caaagttaaa aacgtatggt gggaagttag tcgagaatat
    15361 tgttcaagca actgcaaggg atttacttgc gatttctata gctaggcttg aagcatcagg
    15421 ttttaaaata gttggacatg tccatgatga agtaattgta gaaataccta gaggttcaaa
    15481 tggacttaag gaaatcgaaa ctatcatgaa taagcctgtc gattgggcaa aaggattgaa
    15541 tttgaatagt gacggattta cttctccgtt ttatatgaag gattaggagt gtgattgaat
    15601 gcaacatcaa gcttatatca atgcttctgt tgacattaga attcctacag aagtcgaaag
    15661 tgttaattac aatcagattg ataaagaaaa agagaatttg gcggactatt tatttaataa
    15721 tccaggtgaa ctattaaaat ataacgttat aaatatcaag gttttagatt tagaggtgga
    15781 atgatggcta gaagaaaagt tataagagtg cgtatcaaag gaaaactaat gacattgaga
    15841 gaagtttcag aaaaatatca tatatctcca gaacttctta gatacagata caaacataaa
    15901 atgcgcggcg atgaattatt gtgtggaaga aaagactcaa aatctaaaga tgaagttgaa
    15961 tatatgaaga gtcaaataaa agatgaagaa aaagagagag aaaaaatcag aaaaaaagcg
    16021 attttgaacc tataccaacg aaatgtgaga gcggaatatg aagaagaaag aaagagaaga
    16081 ttgagaccat ggctttatga tggaacgcct caaaaacatt cacgtgatcc gtactggttc
    16141 gatgtcactt ataaccaaat gttcaagaaa tggagtgaag cataatgagc ataatcagta
    16201 acagaaaagt agatatgaat gaaacacaag acaatgttaa acaacctgcg cattacacat
    16261 acggcgacat tgaaattata gatttcatcg aacaagttac ggcacagtat ccaccacaat
    16321 tagcattcgc aataggtaat gcaatcaaat acttgtctag agcaccgtta aagaatggtc
    16381 atgaggattt agcaaaggcg aagttttacg tcgatagagt gtttgacttg tgggaggggt
    16441 aacgatggca acgcaaaaac aagttgatta cgtaatgtca ttacaggagc aactggaatt
    16501 agaagactgc gaaaaatata cagacgaaca agttaaagca atgagtcata aagaagttag
    16561 caatgtgatt gagaactata agacaagcat aaggaatgaa gaactatatt acgaatgcat
    16621 gtcgtttgga ctgcctaatt gttaaaagga gtgacgacca tgacagatag cgcacgtaaa
    16681 gaacgcttaa accaattttt cggctctaaa agatatctgt atcaggataa cgaacgagtg
    16741 gcacatactc atgtagtaaa tggcacgtat tactttcatg ggcatatcgt gccaggttgg
    16801 caaagcgtta aaaagacatt tgatactgct gaagagctcg aaatatatat aaagcaacat
    16861 ggtttggaat acgaggaaca gaagcaacta actttatttt agaggagatg gaaatgatga
    16921 ataaccgcga acaaattgaa caatcaatta tcagtgctag tgcgtataac ggcaatgaca
    16981 cagagggatt actaaaagag attgaagacg tgtataagaa agcgcaagcg tttgatgaaa
    17041 tacttgaagg tttacctaat gctatgcaag atgcactcaa agaagatatt ggtcttgatg
    17101 aagcagtagg gattatgacg gggcaagtgg tctataaata tgaggaggat caggaaaatg
    17161 actaatacat taacaattga tcagttacaa gagttattac aaatacaaaa ggagttcgac
    17221 gatagaatac caacgctgaa cttacgagat agcaaaatag catatgtagt tgaattcttt
    17281 gaatggttta atacattgga aacgtttaag aactggaaga agaaaccagg taagccgtta
    17341 gatgttcagt tagatgaatt agcggacatg ttggcgtttg gattgagtat tgcgaatcaa
    17401 gtaggagtgt catcagaaga gataaaagaa gcgattgaat caagttttaa aaatagtgaa
    17461 tttcacaaca tgtttaattt taaagataaa gaatttgctc aagacgcagt tgttagcaca
    17521 ccacagataa tattcaaaga attttatcct gaccaattgg caattgtaat agcgatagac
    17581 atagctttca acttatattc tatcgaccag ctcattgacg catacaaaaa gaaaatgaaa
    17641 aggaatcatg aaagacaaga tggaacagca gacgcaggaa aaggatacgt gtaaagacat
    17701 cttagatcga gtcaaggagg ttttggggaa gtgacgcaat acttagtcac aacattcaaa
    17761 gattcaacag gacgcaagca tacacacata actaaagcta aaagcaatca aaggtttaca
    17821 gttgttgagg cagagagtaa agaagaagct gagcgcaaat acgaggcaca agttaaaaga
    17881 gatgcagtta ttaaagtggg tcagttattt gaaaatataa gggagtgtgg gaaatgacgg
    17941 aggttaaaat taaaactatt tcagatagag tttattacac aacaacagat ctagcttctg
    18001 acgattatat taatcttgtt atgaatctag tgattgagga ttttcttccg gtcaaagatg
    18061 tgttcaacaa tgaagtatgg gttaaaagag atgagattga atcatttaca tttattaagg
    18121 aggcaaacga tgattaacat acctaaaatg aaattcccga aaaagtacac tgaaataatc
    18181 aaaaaatata aaaataaagc acctgaagaa aaggctaaga ttgaagatga ttttattaaa
    18241 gaaattaaag ataaagacag tgaattttac agtcctacga tggctaatat gaatgaatat
    18301 gaattaaggg ctatgttaag aatgatgcct agtttaattg atactggaga tgacaatgat
    18361 gattaaaaaa cttaaaaata tggatgggtt cgacatcttt attgttggaa tactgtcatt
    18421 attcggtata ttcgcattgc tacttgttat cacattgcct atctatacag tggctagtta
    18481 ccaacacaaa gaattacatc aaggaactat tacagataaa tataacaaga gacaagataa
    18541 agaagacaag ttctatattg tattagacaa caaacaagtc attgaaaatt ccgacttatt
    18601 attcaaaaag aaatttgata gcgcagatat acaagctagg ttaaaagtag gcgataaggt
    18661 agaagttaaa acaatcggtt atagaataca ctttttaaat ttatatccgg tcttatacga
    18721 agtaaagaag gtagataaac aatgattaaa caaatactaa gactattatt cttactagca
    18781 atgtatgagt taggtaagta tgtaactgag caagtgtata ttatgatgac ggctaatgat
    18841 gatgtagagg cgccgagtga ttacgaaaaa atcagagctg aagtttcatg gtaatagcta
    18901 ttatcatttt tgaattaatt atattaatgt gtttagcaat agcactggag gtgttgtaaa
    18961 tatgtggatt gtcatttcaa tcgttttagc tatattttta ttgatcttgt taagtagcat
    19021 ttctcataag atgaaaacca tagaagcatt ggagtatatg aattcttatc ttttcaagca
    19081 gttagtaaaa aataatggtg ttgaaggttt agaagattat gaaaatgaag ttgaacgaat
    19141 tagaaaaaga ttcaaaagct aaagaggggg ctaaagccct ctccaggatt aaaagaagcg
    19201 gatatttttt atttcgtttt tgtaaattaa aactaattta tgtgaaattg tctttgaagt
    19261 cttgataatt aagaaggttg aattttctga gattatatta gttacgggaa aggcttttcc
    19321 tgagtgcaat aaaattaaag ttctcaagtt ttcattttcg tatttatcat aaatgatttc
    19381 gcgtaaagtt ttcaaaattt gtcacctctt taattttatt aaattaatta tacaagaaag
    19441 gagccgaata tgttagacaa agtcactcaa atagaaacaa ttaaatatga tcgtgatgtc
    19501 tcatattctt atgctgctag tcgtttatcc acatattgga ctaatcacaa tatggcttgg
    19561 tctgacttta tgcagaagct agcacaaaca gttagaacta aagaagattt aactgagtac
    19621 aataaaatgt ctaagtctga acaagcagat ataaaagatg ttggcggatt tgtcggtgga
    19681 tatttaaaag aaggcaaacg acgtgctggt caagtcatga atcgttcaat gctaacactt
    19741 gatatcgatt atgctgctca agatatgact gacatattat ctatgtttta tgattttgca
    19801 tattgtttat attcaacaca taagcataga gagataagtc caagactgcg tttagtgatt
    19861 cctttaaaac gaaatgtaaa tgcagatgag tatgaagcta ttgggcgtaa agtcgcagat
    19921 atcgttggca tggattactt cgatgataca acttatcaac cacataggtt aatgtattgg
    19981 ccttcaacta gcaatgatgc agaatttttc tttacctatg aagatttacc tttgttagat
    20041 ccagataaaa tattaaatga atatgttgat tggactgaca cattagaatg gccaacgtct
    20101 tcaaaggaag agagtaagac taaaagatta gcagataagc aaggtgaccc agaagaaaag
    20161 ccgggaattg ttggcgcatt ttgtagagcc tatacgatag aagaagctat atcaactttt
    20221 attcctgact tatacgaaaa acattctact aaccgttata cctatcatga aggttcaact
    20281 gcaggtggat tggtgttata cgaaaataac aagtttgcct attctcatca taatacggat
    20341 cccgttagcg gtatgcttgt gaacagtttt gatttagtac gcatacactt atatggtgct
    20401 caagatgaag acgctaaaac agatactccg gttaatcgac tacctagtta taaagcaatg
    20461 cagcaaagag cgcaaaatga tgaagttgtt aaaaagcaat taattaacga caaaatgtct
    20521 gatgcaatgc aggatttcga tgaaatagta aatagcgatg atgcatggtc tgagacgtta
    20581 gaaattactt cgaaaggtac tttcaaagct agtatcccaa atatagaaat tatattgcgt
    20641 aatgatccaa atttaaaagg aaaaatagca tttaatgaat ttacaaaaca aattgaatgc
    20701 ttagggaaag tgccatggaa tactaatttt aagacacgtc aatggcaaga cggtgatgat
    20761 agcagtttaa gaagttatat cgaaaagatt tatgacatac accattcagg taaaacaaaa
    20821 gatgccatta taagcgtagc aatgcaaaat gcttatcatc cagtaaggga ttatctaaat
    20881 aaaatatcgt gggatggaca taaacgtctt gaaaagttat ttatcaaata cttaggtgtt
    20941 gaagatactg aagtgaatag aacaactacc aaaaaagcat tgactgctgg aattgctcga
    21001 gtaatggagc ctggatgtaa atttgactat atgcttacac tttatggtcc tcaaggtgta
    21061 ggtaaatctg ctttgctaaa aaaattaggt ggtgcatggt tttctgacag tttagtttct
    21121 gttacaggta aagaagccta tgaggcctta caaggcgttt ggctaatgga aatggcagaa
    21181 cttgcagcta caagaaaagc tgaagttgaa gctattaagc atttcatatc taaacaagtt
    21241 gaccggtttc gtgttgctta tggacattat attgaagatt ttccaaggca atgtattttc
    21301 attggtacaa ctaataaagt tgatttctta agagatgaaa ctggtggaag acgtttttgg
    21361 ccaatgactg taaatccaga gagagttgaa gtgaactggt ctaaactaac caaagaagag
    21421 atcgaccaaa tttgggcaga agctaaatat tattatgaac aaggagaaga gttattcctc
    21481 aaccctgaac tagaagaaga aatgcgttca atacaaagca aacatactga ggaatctcca
    21541 tatacaggca ttattgatga atatcttaac acaccaattc ctagcaattg ggatgactta
    21601 actatctttg aacgaagacg attttatcaa ggtgatgttg atatgttacc aacaggaaat
    21661 gtagattacg ttgaaagaaa taaggtctgt gcgcttgaag tgtttgttga atgttttggt
    21721 aaagataagg gagatagtag aggatctatg gaaattagaa agatttcaaa catcttaaga
    21781 caattagaca attggtctgt atatgatggt aataaaagtg ggaaaattcg atttggaaaa
    21841 gattatggtg tacagatagc ttatgtaaga gatgaaagtt tagaagattt aatataataa
    21901 atattgaata aatatacatt ttagagtgtt gtatcagatg ttgcatcatt ttttgagtga
    21961 tgcaacacgt gagtgtaaaa agtaatcgta ggtgttgcat catttttagt gatgcaacat
    22021 tgatgcaaca aatgatacaa cacctctttc ccttctcgct gtaaggttca accctgtttg
    22081 tttccaatgt tgcatcaaat tcactataaa gtttaaaaag tagtgttagg gagtaaagag
    22141 gtataggggt aaccctctaa cagctatttt taaaagtttg gcaagaattg atgcaacatc
    22201 ggaacacaaa tataaatttt gtatacaagg tgaatatatg aaagaatcga cattagaaaa
    22261 atatttagtg aaagagatat caaagctaaa cggtttatgt ttaaaatggg ttgcacctgg
    22321 aacaagaggt gtgccagata gaattattat tatgccagaa ggaaaaacat attttgtaga
    22381 aatgaagcaa gaaaaaggaa agttgcatcc tttacaaaaa tatgtgcata gacaatttga
    22441 aaatagagat cataaagtat atgtgttatg gaataaagaa caagtaaata cttttatcag
    22501 aatggtaggt ggaacatttg gcgattgact tcaaaccaca tagctatcaa aagtatgcaa
    22561 tagataaagt gatagataat gagaaatacg gtctgttttt agatatgggt ctagggaaaa
    22621 cagtatcaac acttacagca tttagtgaat tgcagttgtt agacactaaa aaaatgttag
    22681 ttatagcacc taaacaagtt gctaaagata catgggttga tgaagttgat aagtggaacc
    22741 atttaaatca tctgaaagtg tctttagttt taggaacacc taaagaaaga aatgatgcat
    22801 taaacacaga ggctgatatc tatgtaacca ataaagaaaa tactaaatgg ttatgtgatc
    22861 aatataaaaa agaatggcca tttgacatgg ttgtgattga tgaactgtct acatttaaaa
    22921 gtcctaagag tcaaaggttt aaatctatta aaaagaaatt accactcatt aatagattta
    22981 taggattaac aggaacacct agtccaaata gtttacagga tttatgggct caagtttatt
    23041 tgatagacag aggtgaaaga cttgagtctt cattcagtcg ttatcgagaa aggtacttta
    23101 aaccaactca tcaagttagc gaacatattt ttaagtggga gctaagagac ggatctgaag
    23161 aaaagatata taaacaaata gaagatatat gtttaagcat gaaagcgaaa gattatctgg
    23221 atatgcctga cagagttgat actaaacaaa cagtagtctt atcagaaaaa gaaagaaaag
    23281 tatatgaaga attagaaaaa aactatattt tagaatcgga agaagaagga acagttgtag
    23341 ctcaaaatgg ggcatcatta agtcagaaac tacttcaact atctaacggc gcagtttata
    23401 cagatgagga agatgtaaga cttatacatg ataagaagtt agataagtta gaggaaatta
    23461 tagaggagtc tcaaggccaa ccaatactat tgttttataa cttcaaacac gataaagaaa
    23521 gaatacttca aaggtttaag gaagcaacca cattagacga ttcaaactat aaagaacgtt
    23581 ggaacagtgg agacattaag ttgcttatag cacatccagc aagtgcagga catggattaa
    23641 acttacaaca aggtgggcac attattgttt ggtttggact tacatggtcc ttggaattat
    23701 accaacaagc aaatgctaga ttatacagac aaggacaaaa tcatacgact attattcatc
    23761 atatcatgac cgataacaca atagatcaaa gagtatataa agctttacaa aataaagaac
    23821 taacgcaaga agaattaatg aaagctatta aagcaagaat agctaagcat aagtaatgga
    23881 ggtctaacat ggggaacaca atatatgata tcaagccagg cacatttaaa tatattgaat
    23941 cagaaatata taatttaaat gagaacaaga aagaaataaa aagattgagg ttggaaatac
    24001 ttaatccaac gaaggaacaa gattccaata ttgtatatgg accattacaa aaaggcgaac
    24061 cagttagaac aactgaacta atggcaacta gattattaac taataagatg ttacgaaacc
    24121 ttgaagaaat ggtcgaagca gttgagagtg aatacttaaa gttgcctgaa gatcataaga
    24181 aagtaataag gctcaagtat tggaataaag aaaagaagtt aaagatggaa cagataggac
    24241 atgaatgcca tatgcatcgt aatactgtta ctactatacg aaagaacttt gttaaagcgg
    24301 tagcgtatca tgcaggtatc aaataacatt gtgcaaagat tgtgcaaaag gcctacaaat
    24361 ctgtagtaat atgatagtat cggaaagatg tataaagtta tctaaaagtt atacgacaca
    24421 agtacacgag gcacatcgct atgcggtgtg tcttttgtta tgcaatcaaa gaggtgtaag
    24481 agatgaccaa gcacaataac atttataagc atggtcgtaa gtcatatcaa tacgattggt
    24541 tctatcattc aaaagcatgg aagaagttaa gggagatagc attagataga gataatcatc
    24601 tttgtcaaat gtgtttacgc gaagatattg taacagatgc aaacatagtg catcacatta
    24661 tttatgttga tgaagatttt aacaaagctt tagaattaga taatctaatg tcagtttgtt
    24721 atggctgtca taacaaaatt catgcaaatg gtaatgacaa aagtaatctt aagaaaatta
    24781 gagttctaaa aatttaaata aaaaaacatt taaataaaat tttatagccc cctgcccatt
    24841 ggcttaaaat gttttttcgc cgggtaccgg cgggggccct tcgcttgcaa cgcggataaa
    24901 cttttatgaa agggggtctt tatatgaaat taacaaaaaa acagctgaaa gaatatatag
    24961 aggattataa aaaatctgat gacatattaa ttaatttgta tatagaaacg tatgaatttt
    25021 attgtcggtt aagagatgaa cttaaaaata gtgatttgat gatagagcat acaaacaagg
    25081 ctggtgcgag caatattgtt aagaatccat taagcataga actgacaaaa acagttcaaa
    25141 cactaaataa cttactcaag tctatgggtt taactgcagc acaaagaaaa aagatagttc
    25201 aagaagaagg tggattcggt gactattaaa gttttaaatg aaccttcacc aaaactatta
    25261 acaacatggt atgcagagca agtcactcaa gggaaaataa aaacaagcaa atatgttaaa
    25321 aaagaatgtg agagacacct tagatatcta gaaaatggag gtaaatgggt atttgatgaa
    25381 gaattagcgc accgtcctat tcgattcata gaaaagtttt gtaaaccttc caaaggatct
    25441 aaacgtcaac ttgtattaca accatggcaa cattttatta ttggcagttt gtttggttgg
    25501 gttcataaag aaacaaaact gcgcaggttt aaagaagctt tgatatttat ggggcgaaaa
    25561 aatggtaaaa caactactat atctggtgtt gctaactatg ctgtttctca agatggagaa
    25621 aacggcgctg aaatccatct tttagcaaac gtaatgaaac aagctagaat attattcgat
    25681 gaatctaagg cgatgataaa agctagccca aagcttgata aaaatttcag aacattaaga
    25741 gatgaaatcc attatgacgc aacgatatca aaaattatgc cccaagcatc agatagcgat
    25801 aagttagatg gattgaatac acacatgggg atttttgatg aaattcatga atttaaagac
    25861 tataaattga tttcagttat aaaaaactca agagctgcaa ggttacaacc tcttctcatc
    25921 tacattacga cagcagggta tcaattagat ggtccacttg ttgatatggt agaagcggga
    25981 agagacacct tagatcaaat catagaagac gaaagaactt tttattattt agcatctttg
    26041 gatgatgacg atgatattaa tgattcgtcg aactggataa aagcaaatcc caacttaggt
    26101 gtctctataa atttagatga gatgaaagaa gagtgggaaa aagctaagag aacaccagct
    26161 gaacgtggag attttataac caaaaggttt aatatctttg ctaataatga cgagatgagt
    26221 tttattgatt acccaacact ccaaaaaaat aatgaaattg tttctttaga agagctggaa
    26281 ggcagaccat gcacgattgg ttatgattta tcagaaacag aggactttac agccgcgtgt
    26341 gctacttttg cgttagataa tggtaaagtt gcagttttat cgcattcatg gattcctaag
    26401 cacaaagttg aatattctaa cgaaaaaata ccctatagag aatgggaaga agatggctta
    26461 ttaacagtgc aagataagcc ttatattgac taccaagatg ttttaaattg gataattaag
    26521 atgaatgagc attatgtagt agaaaaaatt acttatgata gagcgaacgc attcaaacta
    26581 aatcaagagt taaaaaatta cgggtttgaa acggaagaaa caagacaagg agctttgacc
    26641 ttgagccctg cattgaagga tttaaaagaa atgtttttag atgggaaaat aatatttaat
    26701 aataatcctt taatgaaatg gtatatcaat aatgttcagt tgaaactaga cagaaacgga
    26761 aactggttgc cgtctaagca aagcagatat cgtaaaatag atggctttgc agcattttta
    26821 aacacatata cagatattat gaataaagtt gtttctgaca agggtgaagg aaacatagaa
    26881 tttattagta ttaaagatat aatgcgttaa ggaggtgaat gttatcgcaa aagagaatat
    26941 tgtcacacgc ataaagaaaa aattgataga caattggatt gatcagtcag cttctaagct
    27001 ttatgacttt agcccatgga aaaataaatc tttttggggt gtaatcaata atacgcttga
    27061 aactaatgaa acgatatttt cagctattac aaagttatct aattcgatgg ctagtttgcc
    27121 cttgaaaatg tatgaagatt ataaagtagt taatacagaa gtatctgatt tacttacagt
    27181 gtcaccgaat aattctctga gcagttttga ttttattaat caaattgaaa caatcagaaa
    27241 tgaaaaaggt aatgcatatg tgctaattga acgagacatc tatcatcaac catcaaagct
    27301 tttcttatta aatccagatg ttgttgaaat gttaattgaa aaccaatcac gtgaacttta
    27361 ttattccatt catgctgcaa ctggaaataa attgattgtt cataatatgg acatgttgca
    27421 ttttaaacac atcgtggcat ctaatatggt gcaaggcatt agtccgattg atgtgttgaa
    27481 gaatacaact gattttgata atgcagtaag aacctttaat cttacagaaa tgcaaaaacc
    27541 tgattctttc atgcttaaat atggttccaa tgtaggtaaa gaaaaaaggc agcaagtgtt
    27601 agaagatttc aaacagtact atgaagaaaa cggtggaata ttattccaag agcctggtgt
    27661 tgaaatcgaa ccgttaccta aaaaatatgt ctctgaagat atagtggcaa gcgagaattt
    27721 aacaagagaa agagtagcta acgtttttca attgccctca gtattcttaa atgcaagatc
    27781 aaatacaaat ttcgcgaaaa atgaagagtt aaacagattt tacttgcagc ataccttatt
    27841 gccaatcgtc aaacagtatg aagaagaatt taatcggaaa ctacttacta aaacagacag
    27901 agaaaaaaat aggtatttta aatttaacgt taaatcttat ttaagggctg atagtgcaac
    27961 acaagcagaa gtgtacttta aagcagttcg tagtggttac tacactataa atgacattag
    28021 agagtgggaa gatttaccac cagttgaagg tggagataag ccgctaataa gcggtgattt
    28081 atacccaatt gacacgccac ttgaattaag aaaatctttg aaaggtggtg ataaaaatgt
    28141 caatgaaagc taagtatttt caaatgaaaa gaaaatcaaa aagtaaaggt gaaatattta
    28201 tttatggtga tattgtaagt gataaatggt ttgaaagtga tgtaactgct acagatttca
    28261 aaaataaact agatgaacta ggagacatca gtgaaataga tgttcatata aattcatctg
    28321 gaggcagtgt atttgaaggg catgcaatat acaatatgct aaaaatgcat cctgcaaaaa
    28381 ttaatatcta tgtcgatgcc ttagcggcat caattgctag tgttatcgct atgagtggtg
    28441 acactatttt tatgcacaaa aatagttttt taatgattca taattcatgg gttatgactg
    28501 taggtaatgc agaagaatta agaaagacag cggatttact tgaaaaaaca gatgctgtta
    28561 gtaattcagc ttatttagat aaagcaaaag atttagatca agaacactta aaacagatgt
    28621 tagatgcaga aacttggctt actgcagaag aagccttgtc tttcggcttg atagatgaaa
    28681 ttttaggagc taatgaaata gctgctagta tctctaaaga gcaatataag cgtttcgaga
    28741 acgtcccaga agatttaaag aaagatgtag acaaaatcac taaaattgat gatgtagata
    28801 catctgaatt ggttgaaaca cctaaagaaa gtatgtcact agaagaaaaa gaaaaaagag
    28861 aaaaaattaa acgcgaatgc gaaattttaa aaatgacaat gaattattag gaggaaatga
    28921 aatgccgaca ttatatgaat taaaacaatc cttaggtatg attggacaac aattaaaaaa
    28981 taaaaatgat gaattgagtc agaaagcaac agatccaaat attgatatgg aagacatcaa
    29041 acaactagaa acagaaaaag caggtttaca acaaagattt aacattgttg aaagacaagt
    29101 gcaagacatt gaagagaaag aaaaagcgaa agttaaagat aaaggagaag cttatcaatc
    29161 tttaagtgat aatgagaaga tggttaaagc taaggcagag ttttatcgtc acgcgatttt
    29221 accaaatgaa tttgaaaaac cttcaatgga ggcacaacgt ttattacacg ctttaccaac
    29281 aggaaatgat tcaggtggag ataagctctt accaaaaaca ctttctaaag aaattgtttc
    29341 agaaccattt gctaaaaacc aattacgtga aaaagctcgt ctaactaaca ttaaaggttt
    29401 agagattcca agagtttcat acactttaga cgatgatgat ttcattacag acgtagaaac
    29461 agcaaaagaa ttaaaagcaa aaggtgatac agtcaagttc actactaata aattcaaagt
    29521 atttgctgca atttcagata ctgtaattca tggatcagat gtagatttag taaactgggt
    29581 tgaaaacgca ctacaatcag gattagcagc taaagagcgt aaagatgcct tagcagtaag
    29641 tcctaaatct ggattagaac acatgtcatt ttataatgga tctgttaaag aagttgaggg
    29701 agcagacatg tatgatgcta ttattaacgc tttagcagat ttacatgaag attatcgtga
    29761 taacgcaaca atttatatgc gatatgcaga ttatgtcaaa attattagtg ttctttcaaa
    29821 tggaacaaca aatttctttg acacaccagc agaaaaagta tttggcaaac cagtagtatt
    29881 tacagatgca gcagttaaac ctattgtggg agatttcaat tattttggaa ttaactatga
    29941 tggaacaact tatgacactg ataaagatgt taaaaaaggc gaatatttgt ttgtattaac
    30001 agcatggtat gatcagcaac gtacattaga cagtgcattc agaattgcaa aagcaaaaga
    30061 aaatacaggt ccattaccca gctaagcccc aaaaggttaa tgtaacagct aaggctaaat
    30121 cagctgtaat atcagccgaa taggggtgat gaaatgagtt tagaagaaat taaattgtgg
    30181 ttgagaattg actataattt cgaaaatgat ttaattgaag gtctcattca atcggctaag
    30241 tctgaattac tattaagtgg ggttccagat tatgacaaag atgacttgga atacccgctt
    30301 ttttgtacag cgattaaata tatcattgca agagattatg aaagtcgtgg atactcaaat
    30361 gaccaatcta gaagcaaggt gtttaatgaa aaaggattgc aaaaaatgat tttgaaatta
    30421 aaaaagtggt aggtgatttt taaatggaat ttaatgaatt taaagatcgc gcgtattttt
    30481 ttcaatatat aaacaaagga ccatatccag atgaagagga aaaaatgaaa ttgtatagtt
    30541 gcttttgtaa aatttataat ccttctatga aagatagaga aattttaaaa gcgactgaat
    30601 caaaatcagg attaaccata attgtcaggt cttctaaaac tgaatatcta ccacaaacaa
    30661 atcacttagt taaaattgac agtgcattat attccgataa attattcaac attgtagaaa
    30721 taagaattga tacaccagat attggctata atacagtggt tttatcagaa aaatgagtgt
    30781 agaaattaaa gggatacctg aagtgttgaa taaattagaa tcggtatacg gtaaacaagc
    30841 aatgcaggct aagagtgata aagctttaaa tgaagcatct gaatttttta taaaggcttt
    30901 aaagaaagag ttcgagagct ttaaagatac gggtgccagt atagaagaaa tgactaaatc
    30961 taagccttat acaaaagttg gcagtcaaga aagggctgtt ttaattgaat gggtaggccc
    31021 tatgaatcgc aaaaacatta ttcacttgaa tgaacatggt tatacaagag atggaaaaaa
    31081 atatacacca agaggttttg gagttattgc aaaaacatta gctgctagcg aacgtaagta
    31141 tagagaaatt ataaaaaagg agttggccag ataaatgaat atattaaaca ccataaaagg
    31201 aattttatta tctgatgcag agctcaaaac acatataaat tctagaatat actattacaa
    31261 agtcactgaa aacgctgaaa cttccaaacc ctttgttgtt attacacctg tttatgattt
    31321 gccttcagac tttatgtctg ataaatatct cagtgaagaa tacttaattc aaatagatgt
    31381 agaatcttca aataatcaga aaacaattga tataacaaaa cgaataagat acctgttata
    31441 tcaacaaaat ttaattcaag catctagtca gttagatgct tattttgaag aaactaaacg
    31501 ttatgtgatg tcgagacgat atcaaggcat acccaaaaat atatattata aaaatcagcg
    31561 catcgaatag gtgtgctttt taatttttaa ggaggaaata agcaatggca gaaggacaag
    31621 gttcttataa agtaggtttt aaaagattat acgttggagt ttttaaccca gaagcaacaa
    31681 aagtagttaa acgcatgaca tgggaagatg aaaaaggtgg tacagttgac ctaaatatca
    31741 caggtttagc accagattta gtagatatgt ttgcatctaa caaacgtgta tggatgaaaa
    31801 aacaaggtac taatgaagtt aagtctgaca tgagtatttt caatattcca agtgatgatt
    31861 taaacacagt tattggacgt actaaagata aaaatggtac atcttgggta ggagagaata
    31921 caagagcacc gtatgtaaca gtaattggcg aatcggaaga tggtttaaca ggtcagccgg
    31981 tatatgtagc cttacttaaa ggtactttta gtttagattc aattgaattt aaaacacgag
    32041 gtgaaaaagc agaagcccca gaacctacaa aattaacagg tgactggatg aatagaaaag
    32101 ttgatgttga tggaacgtca caaggtattg tatacggtta tcatgaaggt aaagaaggag
    32161 aagcagaatt cttcaaaaaa gtattcgttg gatacacgga cagtgaagat cattcagagg
    32221 attctgcagg ttcgttaccc agctaatccc caaaatgttg aagtagcagt taattcaaaa
    32281 tctgcaacag tttcagcaga ataggggctt tcaaaataaa tcaaaggaga ataatttatg
    32341 actaaaactt taaaggttta taaaggagac gacgtcgtag cttctgaaca aggtgaaggc
    32401 aaagtgtcag taactttatc taatttagaa gcggatacaa cttatccaaa aggtacttac
    32461 caagtggcat gggaagaaaa tggtaaagaa tctagtaaag ttgatgtacc tcaattcaaa
    32521 accaatccaa ttctagtctc aggcgtatca tttacaccag aaactaaatc aattatggta
    32581 aataccgatg acaatgttga gccaaacatt gcaccaagca cagcaacgaa taaaatattg
    32641 aaatatacaa gtgaacatcc agaatttgtt actgtagatg aaaatacagg agcaattcac
    32701 ggtgtagctg aaggtacttc agtaatcact gctatgtcta ctgatggaag cgataagtca
    32761 ggacaaattt cagtgacagt aacaaacgga tagggattta aggcgcagta tatctgcgtc
    32821 ttttttattt gaataaaagg agctaataca atgattaaat ttgaaattaa agatcgtaaa
    32881 acaggaaaaa cagagagcta tacaaaagaa gatgtaacaa tgggcgaagc agaaaaatgc
    32941 tatgagtatt tagaattagt aaatcaagag aataaaaaag aagcacctaa cgcaacaaaa
    33001 atgagacaaa aagagcgaca gttattagta gatttattta aagatgaagg attgactgaa
    33061 gaagatgttc tgaacaagat gagtactaaa acttatacaa aagccttaca agatatattt
    33121 cgagaaatca atggtgaaga tgaagaagat tcagaaactg aaccagaaga gatgggaaag
    33181 acagaagaac aatctcaata aaagacattt tatcgaacat taagaaaata caacgtttct
    33241 gtatggagca gtatgggtgg acattaactg aagtcagaaa acagccgtat gtaaaacttt
    33301 tagaaatact taatgaagag aataaagaag agactgaaga aaaacaaagt gaacaaaaag
    33361 tcattacagg tacggattta agaaaacttt ttggaagcta gaaaggaggt taatatgaat
    33421 gaaaaagtag aaggcatgac cttggagctg aaattagacc atttaggtgt ccaagaatgc
    33481 atgaaaggtt taaagcgaca attaggtgtt gttaatagtg aaatgaaagc taatctgtca
    33541 gcatttgata agtctgaaaa atcaatggaa aaatatcagg cgagaattaa ggggttaaat
    33601 gataggctta aagttcaaaa aaagatgtat tctcaagtag aagatgagct taaacaagtt
    33661 aacgctaatt accaaaaagc taaatccagt gtaaaagatg ttgagaaagc atatttaaag
    33721 ttagtagaag ccaataaaaa agaaaaatta gctcttgata aatctaaaga agccttaaaa
    33781 tcatcgaata cagaacttaa aaaagctgaa aatcaatata aacgtacaaa tcaacgtaaa
    33841 caagatgcgt atcaaaaact taaacagttg agagatgcag aacaaaagct taagaatagt
    33901 aaccaagcta ctactgcaca actaaaaaga gcaagtgacg cagtacagaa gcagtccgct
    33961 aagcataaag cacttgttga acaatataaa caagaaggca atcaagttca aaaactaaaa
    34021 gtgcaaaatg acaatctttc aaaatcaaat gataaaattg aaagttctta cgctaaaact
    34081 aatactaaat taaagcaaac agaaaaagaa tttaatgatt taaacaatac tattaagaat
    34141 catagcgcta atgtcgcaaa agctgaaaca gctgttaata aagaaaaagc tgctttaaat
    34201 aatttggagc gttcaataga taaagcttca tccgaaatga agacttttaa caaagaacaa
    34261 atgatagctc aaagtcattt cggtaaactt gcaagtcaag cggatgtcat gtcaaagaaa
    34321 tttagttcta ttggagacaa aatgacttcc ctgggacgta caatgacgat gggcgtatct
    34381 acaccaatta ctttagggtt aggtgcagca ttaaaaacaa gtgcagactt tgaaggccaa
    34441 atgtctcgag ttggagcgat tgcgcaagca agcagtaaag acttgaaaag catgtctaat
    34501 caagcagttg acttaggagc taaaaccagt aaaagtgcta acgaagttgc taaaggtatg
    34561 gaagaattgg cagctttagg ctttaatgcc aaacaaacaa tggaggctat gccaggtgtt
    34621 atcagtgcag cagaagcaag tggtgcagaa atggctacaa ctgcaactgt aatggcttca
    34681 gcgattaact ctttcggttt aaaagcatct gatgcaaatc atgttgctga tttacttgcg
    34741 agatcagcaa atgatagtgc tgcagatatt cagtacatgg gagatgcatt gaagtatgct
    34801 ggtactcctg caaaagcatt aggagtttca atagaggaca cttccgcagc aattgaagtt
    34861 ttatctaact caggtttaga gggttctcaa gcaggtactg ccctaagagc ttcatttatc
    34921 aggctagcta atccaagtaa aaatacagct aaggaaatga aaaaattagg tattcatttg
    34981 tctgatgcta aaggtcaatt tgttggcatg ggtgaattga ttagacagtt ccaagataat
    35041 atgaaaggca tgacgagaga acaaaaacta gctacagtgg ctacaatagt tggtactgaa
    35101 gcagcaagtg gatttttagc cttgattgaa gcgggaccag ataaaattaa tagctatagt
    35161 aaatccttaa agaattccaa tggcgaaagt aaaaaagcag cagatttgat gaaagataat
    35221 ctcaaaggcg ctctggaaca attaggtggc gcttttgaat cattagcaat cgaagtcggt
    35281 aaagatttaa cgcctatgat tagagcagga gcggaaggtt taacaaaatt agttgatgga
    35341 tttacacatc tccctggttg ggttagaaaa gcttcagtag gattagcact ttttggtgca
    35401 gcaattggac ctgcagttct tgctggaggg ttattaatac gtacagttgg aagtgctgct
    35461 aaaggatatg cgtcattaaa tagacgtatt gctgaaaata caatcctttc aaatactaat
    35521 tcaaaagcaa tgaaatcttt aggtcttcaa acattatttc ttggttctac aacaggaaaa
    35581 acgtcaaaag gctttaaagg gttagccgga gctatgatgt ttaatttaaa acctataaat
    35641 gttttgaaaa attctgcaaa gctagcaatt ttaccgttca aacttttgaa aaacggttta
    35701 ggattagctg caaaatcttt atttgcagta agtggaggcg caagatttgc gggtgtagcc
    35761 ttaaggtttt taacaggacc tataggtgct acaataactg ctattacaat tgcgtataaa
    35821 gtttttaaaa ccgcatatga tcgtgtggaa tggttcagaa acggtattaa cggtttagga
    35881 gaaactataa agttttttgg tggtaaaatt attggcggcg ctgttagaaa gctaggagag
    35941 tttaaaaact atcttggaag tatcggcaaa agcttcaaag aaaagttttc aaaagatatg
    36001 aaagatggtt ataaatcatt aagcgacgat gaccttctca aagtaggagt caacaagttt
    36061 aaaggattta tgcaaaccat gggcacagct tctaaaaaag cgtctgatac tgtaaaagtg
    36121 ttagggaaag gtgtttcaaa agaaacagaa aaagctttag aaaaatatgt gcattattct
    36181 gaagaaaata gcagaatcat ggaaaaagta cgtttaaact cgggtcagat atcagaagac
    36241 aaagcaaaaa aacttttgaa aattgaaacg gatttatcta ataaccttat agctgaaata
    36301 gaaaaaagaa ataaaaagga actcgaaaaa actcaagaac ttattgataa gtatagtgca
    36361 ttcgatgaac aagaaaagca aaacatttta actcgaacta aagaaaaaaa tgacttgcga
    36421 attaaaaaag agcaagaact caatcagaaa atcaaagaat tgaaagaaaa agctttgagt
    36481 gatggtcaga tttcagaaaa tgaaagaaaa gaaattgaaa agcttgaaaa tcaaagacgt
    36541 gatatcactg ttaaagaatt gagtaagact gaaaaagagc aagagcgtat tttagtaaga
    36601 atgcaaagaa acagaaatgc ttattcaata gacgaagcga gcaaagcaat taaagaagca
    36661 gaaaaagcaa gaaaagcaag aaaaaaagaa gtggacaagc aatatgaaga tgatgtcatt
    36721 gctataaaaa ataacgtcaa cctttctaag tctgaaaaag ataaattgtt agctattgct
    36781 gatcaaagac ataaagatga agtaagaaaa gcaaaatcta aaaaagatgc tgtagtagat
    36841 gttgttaaaa agcaaaataa agatattgat aaagaaatgg atttatccag tggacgtgta
    36901 tataaaaata ctgaaaagtg gtggaatggc cttaaaagtt ggtggtctaa ctttagagaa
    36961 gaccaaaaga aaaaaagcga taaatacgct aaagaacaag aagaaacagc tcgtagaaac
    37021 agagaaaata taaagaaatg gtttggaaat gcttgggacg gcgtaaaaag taaaactggc
    37081 gaagccttta gtaaaatggg cagaaatgct aatcattttg gcggcgaaat gaaaaaaatg
    37141 tggagcggaa tcaaagggat tccaagcaaa ttaagttcag gttggagctc agccaaaagt
    37201 tctgtaggat accacactaa ggctatagct aatagtactg gtaaatggtt tggaaaagct
    37261 tggcaatctg ttaaatcgac aacaggaagt atttacaatc aaactaagca aaagtattca
    37321 gatgcttcag ataaagcttg ggcgcattca aaatctattt ggagaggcac atcaaaatgg
    37381 tttagcaatg catataaaag tgcaaagggc tggctaacgg atatggctaa taaatctcgc
    37441 gcgaaatggg ataatatttc tagtacagct tggtcgaatg caaaatccgt ttggaaagga
    37501 acatcgaaat ggtttagtaa ctcatacaaa tctttaaaag attggactgg ggatatgtat
    37561 tcaagagccc acgatcgttt tgatgcaatt tcaagttcgg catggtctaa cgctaaatca
    37621 gtatttaatg gttttagaaa atggctatcc aaaacatatg attggattag agatattggt
    37681 aaagacatgg gaagagctgc ggctgattta ggtaaaaatg ttgctaataa agctattggc
    37741 ggtttgaata gcatgattgg cggtattaat aaaatatcta aagccattac tgataaaaat
    37801 ctcatcaagc caatacctac attgtctact ggtactttag caggaaaggg tgtagctacc
    37861 gataattcgg gagcattaac gcaaccgaca tttgctgtat taaatgatag aggttctgga
    37921 aacgccccag gtggtggagt tcaagaagta attcacaggg ctgacggaac attccatgca
    37981 ccccaaggac gagatgtggt tgttccacta ggagttgggg atagcgtaat aaatgctaat
    38041 gacactctga agttacagcg tatgggtgtt ttaccaaagt ttcatggagg tacgaaaaag
    38101 aaagattggc tagaccaact taaaggtaat ataggtaaaa aagcaggaga atttggagct
    38161 acagctaaaa acacagcgca taatatcaaa aaaggtgcag aagaaatggt tgaagcagca
    38221 ggcgataaaa tcaaagatgg tgcatcttgg ttaggcgata aaatcggcga tgtgtgggat
    38281 tacgtacaac atccagggaa actagtaaat aaagtaatgt caggtttaaa tattaatttt
    38341 ggaggcggag ctaacgctac agtaaaaata gctaaaggcg catactcatt gctcaaaaag
    38401 aaattagtag acaaagtaaa atcgtggttt gaagatttcg gtggtggagg cgatggaagc
    38461 tatctatttg aatatccaat ctggcaaaga tttggacgct acacaggtgg acttaacttt
    38521 aatggcggtc gtcactatgg tatagacttt ggtatgcctt ctggaacaaa cgtttatgcc
    38581 gttaaaggtg gtatagcaga taaggtatgg actgattacg gtggcggtaa ttctatacaa
    38641 attaagactg gtgctaatga atggaactgg tatatgcatt tatctaagca attagcaaga
    38701 caaggccaac gtattaaagc tggtcaactg atagggaaat caggtgctac aggtaatttc
    38761 gttagaggag cacacttaca tttccaattg atgcaagggt cacatccagg gaatgataca
    38821 gctaaagatc cagaaaaatg gttgaagtca cttaaaggta gtggcgttcg aagtggttca
    38881 ggtgttaata aggctgcatc tgcttgggca ggcgatatac gtcgtgcagc aaaacgaatg
    38941 ggtgttaatg ttacttcggg tgatgtagga aatattatta gcttgattca acacgaatca
    39001 ggaggaaatg caggtataac tcaatctagt gcgcttagag acatcaacgt tttacagggc
    39061 aatccagcaa aaggattgct tcaatatatc ccacaaacat ttagacatta tgctgttaga
    39121 ggtcacaaca atatatagtg gttacgatca gttattagcg ttctttaaca acagatattg
    39181 gcgctcacag tttaacccaa gaggtggttg gtctccaagt ggtccaagaa gatatgcgaa
    39241 tggtggtttg attacaaagc atcaacttgc tgaagtgggt gaaggagata aacaggagat
    39301 ggttatccct ttaactagac gtaaacgagc aattcaatta actgaacagg ttatgcgcat
    39361 catcggtatg gatggcaagc caaataacat cactgtaaat aatgatactt caacagttga
    39421 aaaattgttg aaacaaattg ttatgttaag tgataaagga aataaattaa cagatgcatt
    39481 gattcaaact gtttcttctc aggataataa cttaggttct aatgatgcaa ttagaggttt
    39541 agaaaaaata ttgtcaaaac aaagtgggca tagagcaaat gcaaataatt atatgggagg
    39601 tttgactaat taatgcaatc ttttgtaaaa atcatagatg gttacaagga agaagtaata
    39661 acagatttta atcagcttat atttttagat gcaagggctg aaagtccaaa caccaatgat
    39721 aacagtgtaa ctattaacgg agtagatggt attttaccgg gcgcaattag ttttgcgcct
    39781 ttttcattag tattaaggtt tggctatgat ggtatagatg ttatagattt aaatttattt
    39841 gagcattggt ttagatctgt gtttaatcgc agacatcctt attatgttat tacttctcaa
    39901 atgcctggtg ttaaatatgc agtgaataca gctaatgtta catctaattt aaaagatggt
    39961 tcttcaactg aaattgaagt aagtttaaat gtttataaag ggtattctga atcagttaat
    40021 tggaccgata gcgagttctt attcgactct aattggatgt ttgaaaatgg aattcctctt
    40081 gatttcacac ctaaatatac tcatacatca aatcaattta ctatttggaa cggttctact
    40141 gatacgataa atccacgatt caagcacgat ttgaaaatat taattaattt aaatgcgagt
    40201 ggaggatttg aactggttaa ctatacaaca ggtgatattt ttaagtacaa caaaagtata
    40261 gataaaaaca ctgattttgt tttagatggt gtgtatgcat atcgagatat aaatagagtg
    40321 ggaattgata caaatagagg cattataaca ttagcgccag gtaaaaatga atttaagatt
    40381 aaaggagacg tcagtgatat taaaactaca tttaagtttc cttttattta taggtaggtg
    40441 atttaatgga ttatcatgat catttatcag taatggattt taatgaattg atttgtgaaa
    40501 atttactaga tgtagattat ggttctttta aagaatatta tgaactgaat gaagctaggt
    40561 acatcacctt tacagtttat agaactactc ataatagttt tgtttttgat ttattgattt
    40621 gtgaaaactt cataatttat catggtgaaa aatatacaat taagcagaca gcgccaaagg
    40681 ttgaaggtga taaagttttt attgaagtta cggcatatca cataatgtat gaatttcaaa
    40741 atcactcagt ggaatcaaat aagcttgatg acgacagtag cgaaactggt aaaacgccag
    40801 aatactcttt agatgagtac ttaagatatg gatttgcaaa tcaaaaaact tcggtcaaaa
    40861 tgacctataa aataattgga gattttaagc gaaaagtacc gattgacgaa ttaggtaaca
    40921 aaaacggctt agaatactgt aaagaagcgg tagacctgtt tggctgtata atttacccaa
    40981 atgatacaga gattggtttt tattctcctg aaacatttta tcaaagaagc gagaaagtga
    41041 ttcgatatca atataatact gatactgtat ctgcaactgt cagtacattg gaattaagaa
    41101 cagctataaa agtttttgga aaaaagtata cagctgagga aaagaaaaat tataatccta
    41161 ttagaacaac tgacattaaa tattcaaatg gttttataaa agaaggtact tatcgtaccg
    41221 aaacaattgg gtctaaagct actattaact ttgattgcaa gtatggtaat gaaacagtta
    41281 gatttacaat aaaaaagggc tctcaaggtg gaatatataa gttgatttta gacggcaagc
    41341 aaattaagca aatttcttgt tttgctaagt cggttcagtc tgaaacaata gatttaataa
    41401 aaaatattga taaaggcaag cacgttttag aaatgatatt tttaggagaa gaccccaaaa
    41461 atagaattga tatatcttca aataaaaaag ctaagccttg tatgtatgtt ggaactgaaa
    41521 aatcaacagt cttaaattta attgctgata attcaggtcg caatcaatac aaagcaattg
    41581 tcgactacgt cgcagatagt gcaaagcagt ttgggattcg atatgctaat acgcaaacaa
    41641 atgaagatat cgaaacacag gataagctgt tagaatttgc aaaaaagcaa ataaatgata
    41701 ctcctaagac tgaattagat gttaattata taggttatga aaaaatagag ccaagagata
    41761 gcgtattttt tgttcatgaa ttaatgggat ataacactga attaaaggtt gttaaacttg
    41821 ataggtcaca tccatttgta aacgcaatag atgaagtgtc tttcagcaat gaaataaaag
    41881 atatggtaca aattcaacaa gcacttaaca gacgagttat tgcacaagat aatagatata
    41941 actatcaagc aaatcgtata aatcatttat acactagtac tttgaattct cctttcgaga
    42001 caatggatat agggagtgta ttaatataat ggcaacagaa gaagttaaaa tcaaagcgct
    42061 acttgaaaac gataaacagt actttccagc tacacactgg aaagctataa atgggatacc
    42121 ttatgcaggc agtagtgata ttgatggatt gcctcaagac ggtatcattt cggtagatga
    42181 taaaaataaa ttagataaat taaaaatagg cgaagcagga attattcaaa atagcattgt
    42241 acagaaatcc ccaaacggta aattgtggaa aataacagtt gacgatagtg ggaaacttgg
    42301 tacagtgcta ttttattaga aaggaaggtg cattatggaa aatttgtatt taataaagga
    42361 tttgggagct ttagcaggtc gagattatag agctaaagaa attcaaaacc tgcaaagaat
    42421 agagcaattt gcgcttggct tgacaacaga gtttaagttg catcagaaag ctaaaacaat
    42481 gcaacacttc gctgagcaaa tttattataa tggtagatcg caagcagcag taaacaaatc
    42541 tttacaaagt caaattaacg cacttgttgt ggcaccacgt aataacagtg ctaatgagat
    42601 tgttcaagct cgagttaatg taaacggcga aacctttgac acattaaaag aacatttaga
    42661 cgattgggaa accaaaactc aaattaataa agaggaaact ataagagaat taaataagac
    42721 caaacaagaa attcttgata tcgagtatcg ttttgaacct gataagcaag agtttttatt
    42781 tgtgacagaa cttgcacctc ttacaaatgc agtaatgcaa tccttctggt ttgataatag
    42841 aacaggcata gtatacatga cacaagctag aaataatggc tatatgctaa gtcgtttaag
    42901 acctaatggt caatttatag acagctcatt gattgtaggt gggggtcatg gtacacataa
    42961 cggttataga tatattgatg atgagttatg gatttatagt tttatcttaa atggtaataa
    43021 tgagaataca ttagttcgtt tcaagtatac gcctaatgtg gaaattagct atggcaagta
    43081 tggtatgcaa gatgtattta caggacaccc agaaaaaccc tacatcaccc ctgtcataaa
    43141 tgaaaaagaa aataaaattc tatacagaat tgagagacct agaagtcagt gggaacttga
    43201 aaactcaatg aattatatag agataagaag tttagacgat gttgataaaa atattgataa
    43261 agttttgcat aaaatcagta tccctatgag actaacaaac gaaacccaac caatgcaggg
    43321 tgtgactttt gatgaaaaat acttgtattg gtatacagga gacagtaatc caaataatag
    43381 aaactattta acggctttcg atttagaaac aggagaagaa gcgtatcagg ttaatgctga
    43441 ctatggtgga acactagatt catttcctgg cgaatttgcg gaagcagaag gtttgcaaat
    43501 atactatgac aaagatagtg gtaaaaaagc tttgatgcta ggtgttactg tcggtggtga
    43561 tggaaataga acacatcgta ttttcatgat tgggcaaaga ggtattttag aaatacttca
    43621 ctcaagaggc gttcctttta tcatgagtga cacaggtggt agagttaaac ctttaccaat
    43681 gaggcctgat aaacttaaga atcttgggat gttaacagag ccaggtcttt actatttata
    43741 cactgatcat acagttcaaa tcgatgattt cccattacca agagaatggc gtgatgcagg
    43801 ttggttcttg gaagttaagc caccacaaac tggcggtgat gtaattcaga tattgacgcg
    43861 taatagttat gcaaggaata tgatgacttt tgaaagggtg ctttctggaa gaactggaga
    43921 catttcggac tggaattatg tgcctaaaaa tagtggtaaa tgggagagag taccttcatt
    43981 catcacaaaa atgtcagata ttaacatagt aggcatgtcg ttttatttaa ctacggatga
    44041 tacaaaacgt tttacagatt ttccaactga acgtaaaggg gtagctggtt ggaacttata
    44101 tgtagaagct tcaaacacag gtggctttgt tcataggcta gttcgtaata gtgttacagc
    44161 atctgctgag atactattga aaaattatga tagtaaaaca agttcagggc catggacttt
    44221 acacgaaggg agaattataa gttaatgagt aatttagaga aatctgtagc tataaattta
    44281 gaaaacacag cgcattatga aaatatttca aatctagata taacttttag aacaggagag
    44341 agtgattctt ctgttcttct ttttaatatc attaaaaata atcaaccgtt attactgagt
    44401 gaagaaaata tcaaagcacg aatagcgatt cgaggtaaag gagtaatggt agttgctcca
    44461 ctagaaatat tagatccatt taaaggtatt ttaaaatttc aattacctaa tgatgtaatt
    44521 aaaagagatg gaagttatca agctcaagtt tcggttgcag aattaggtaa ttcagacgtg
    44581 gtagttgtag agagaactat cacatttaac gttgaaaaaa gtttgtttag caagattccc
    44641 tctgaaacaa aactacacta tattgttgag tttcaagaat tagaaaaaac tattatggat
    44701 cgcgcgaaag caatggacga ggctataaaa aatggtgagg attatgcgag tctgattgaa
    44761 aaagctaaag aaaaaggtct atcagatatt caaatagcaa aatcttcaag tattgatgaa
    44821 ttaaagcaac ttgctaatag ccgtatatct gatttggaaa ataaagcgca agcatattca
    44881 agaacattcg atgagcaaaa gcgatatatg gatgagaaac atgaagcctt caagcagtca
    44941 gtgaatagtg gtggtttagt cacaagtggt tctacttcaa attggcaaaa agctaagatt
    45001 actaaagatg atggtaagat aatgcagatt actggatttg attttaataa tccagaacaa
    45061 agaataggtg attcaaccca atttatttat gtttcgcaag ctataaatta tccaagagat
    45121 gttagtacta acggtactgt cgaatattta gtagtaactt cagattacaa gcgtatgact
    45181 tatcgaccga acggtacaaa taaagtgttt gttaaaagaa aagaagcggg ttcatggtct
    45241 gagtggtcag aattagctat taatgattac aatacacctt ttgaaactgt tcaaagtgcc
    45301 caatcaaaag ctaatatggc cgaaagtaac gctaaattat acgcagatga caagtttaat
    45361 aaaaggtatt cggttatttt tgatggaaca gcaaatggtg tgggctctac attgtactta
    45421 aatgagagtt tagaccaatt tattttatta attttttatg ggacttttcc aggtggtgac
    45481 tttacagagt ttggcagtcc ttttggagga ggaaagattt cattgaatcc ctcaaatctt
    45541 ccagatggtg atggaaatgg tggaggtgtt tatgagtttg gattaactaa atctagtcgt
    45601 acatctttaa ctatatcaaa cgatgtctat ttcgacttag gaagtcaaag aggctctggt
    45661 gcgaacgcaa atagagggac aattaacaaa attataggag tgagaaaata atgcaaatat
    45721 tagttaacaa gcgtaatgag ataatttcat acgctatcat tggcggcttt gaagaaggta
    45781 ttgatattga aaatttacca gaaaatttct ctcaagtttt tagacctaaa gcctttaaat
    45841 attcaaatgg ggaaatagtt tttaacgaag attattcaga agaaaaagat gacttgcatc
    45901 aacagattga cagtgaagaa caaaacacag tcgcttctga tgacatctta cgaaaaatgg
    45961 ttgctagtat gcagaaacaa gttgttcaaa gtacaaagtt atcgatgcaa gttaataagc
    46021 aaaatgcact aatggcaaaa caacttgtga cacttaataa aaaattagaa gaggttaaag
    46081 gagagactga aaatgcttaa attaatttca ccaacattcg aagatattaa aacatggtat
    46141 caattgaaag aatatagtaa agaagatata gcgtggtatg tagatatgga agttatagat
    46201 aaagaggaat atgcaattat tacaggagaa aagtatccag aaaatctaga gtcataggtt
    46261 ataatcttat ggctttttaa tttgaataaa gtgggtggtg taatgtttgg atttaccaaa
    46321 cgacacgaac aagattggcg tttaacgcga ttagaagaaa atgataagac tatgtttgaa
    46381 aaattcgaca gaatagaaga cagtctgaga acgcaagaaa aaatttatga caagttagat
    46441 agaaatttcg aagaactaag gcgtgacaaa gaagaagatg aaaaaaataa agagaaaaat
    46501 gctaaaaata ttagagacat caagatgtgg attctaggat taatagggac gattctaagt
    46561 acatttgtta tagccttgtt aaaaactatt tttggcattt aaaggaggtg atcaccatgc
    46621 ttaagggaat tttaggatat agcttttggt cgtgtttctg gtttggtaag tgtaagtaat
    46681 atttaagagt cagtgcttcg gcactggctt tttattttgg ataaaaggag caaacaaatg
    46741 gatgcaaaag taataacaag atacatcgta ttgatcttag cattagtaaa tcaattctta
    46801 gcgaataaag gtataagtcc gataccagta gatgaagaaa gtgtttcatc gattatctta
    46861 acagttgttg ctttatatac tacatataaa gataatccaa catctcaaga agggaaatgg
    46921 gcgaatcaaa aattaaagaa atataaagct gaaagtaaat atagaaaagc aacagggcaa
    46981 gcgccaatta aagaagtaat gacacctacg aatatgaacg acacaaatga tttagggtag
    47041 gtgttgacca atgttgataa caaaaaacca agcagaaaaa tggtttgata attcattagg
    47101 gaagcagttc aatcctgatt tgttttatgg atttcagtgt tatgattacg ccaatatgtt
    47161 ctttatgtta gcgacaggcg aaaggctgca aggtttatat gcttataata tcccgtttga
    47221 taataaagca aagattgaaa aatatggtca aataattaaa aactatgaca gctttttacc
    47281 gcaaaagttg gatattgtcg ttttcccgtc aaagtatggt ggcggagctg gacacgttga
    47341 aattgttgag agcgcaaatt taaacacttt cacatcattt ggtcaaaact ggaacggtaa
    47401 aggttggact aatggcgttg cgcaacctgg ttggggtcct gaaactgtga caagacatgt
    47461 tcattattat gacaatccaa tgtattttat taggttaaac ttccctaaca acttaagcgt
    47521 tggcaataaa gctaaaggta ttattaagca agcgactaca aaaaaagagg cagtaattaa
    47581 acctaaaaaa attatgcttg tagccggtca tggttataac gatcctggag cagtaggaaa
    47641 cggaacaaac gaacgcgatt ttatacgtaa atatataacg cctaatatcg ctaagtattt
    47701 aagacatgca ggacatgaag ttgcattata cggtggctca agtcaatcac aagatatgta
    47761 tcaagatact gcatacggtg ttaatgtagg caataaaaaa gattatggct tatattgggt
    47821 taaatcacag gggtatgaca ttgttctaga aatacattta gacgcagcag gagaaagcgc
    47881 aagtggtggg catgttatta tctcaagtca attcaatgca gatactattg ataaaagtat
    47941 acaagatgtt attaaaaata atttaggaca aataagaggt gtaacacctc gtaacgattt
    48001 actaaatgtt aatgtatcag cagaaataaa tatcaattat cgcttatctg aattaggttt
    48061 tattactaat aaaaatgata tggattggat taagaaaaat tacgacttgt actctaaact
    48121 aatagctggt gcgattcatg gtaagccaat tggtggagtg gtagctagtg aggtcaaagc
    48181 gccagttaaa aacgaaaaga atccgccagt gccagcaggt tatacactcg ataagaataa
    48241 tgtcccttat aaaaaagaac aaggcaatta cacagtagct aatgttaaag gtaataatgt
    48301 aagagacggt tattcaacta attcaagaat tacaggggta ttacccaaca acacaacaat
    48361 tacgtatgac ggtgcatatt gtattaatgg ttatagatgg attacttata ttgctaatag
    48421 tggacaacgt cgctatattg cgaccggaga ggtagacata gcaggcaacc gaataagcag
    48481 ttttggtaag tttagtgcag tttgataatt gtatatgatg aatcttaggc aggtacttcg
    48541 gtacttgcct attatttaaa attaataaac agttaatttt tacatgaata tattaaattt
    48601 taaaaaaaca aacgttttta gtatataaat tattttgtgt tcgtattgtg tgctatgatt
    48661 aaaaagttgt tatggtcaac tatatcgtgg ttttatgttt attatcaatc aaaatataaa
    48721 ttatttataa tttgtttggt aatgaacggg tttttt
//
LOCUS       DALAGF010000007        45984 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.007, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000007 DALAGF010000000
VERSION     DALAGF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 45984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45984
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.007"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(133..1101)
                     /locus_tag="O2692_000380"
     CDS             complement(133..1101)
                     /locus_tag="O2692_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCZ4298028.1"
                     /translation="MLLNLLLIFILIVICPLIFNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWSMFVTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFI
                     STIQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTE
                     FVTPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDI
                     LMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGS
                     HTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
     gene            1296..1751
                     /locus_tag="O2692_000381"
     CDS             1296..1751
                     /locus_tag="O2692_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ4298029.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            1991..3373
                     /locus_tag="O2692_000382"
     CDS             1991..3373
                     /locus_tag="O2692_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298030.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANISVTLKGDTKLNDSSK
                     KVTINDHEMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            3404..3532
                     /locus_tag="O2692_000383"
     CDS             3404..3532
                     /locus_tag="O2692_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298031.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCGKIYEVLRFISFYTFYKL"
     gene            3642..4850
                     /locus_tag="O2692_000384"
     CDS             3642..4850
                     /locus_tag="O2692_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCZ4298032.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNHSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
     gene            complement(5080..5631)
                     /locus_tag="O2692_000385"
     CDS             complement(5080..5631)
                     /locus_tag="O2692_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCZ4298033.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQN"
     gene            5752..6399
                     /locus_tag="O2692_000386"
     CDS             5752..6399
                     /locus_tag="O2692_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCZ4298034.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVAIVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            6412..8343
                     /locus_tag="O2692_000387"
     CDS             6412..8343
                     /locus_tag="O2692_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCZ4298035.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNK
                     DEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMHELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLDADNSINHE"
     gene            complement(8589..9227)
                     /locus_tag="O2692_000388"
     CDS             complement(8589..9227)
                     /locus_tag="O2692_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298036.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADVFKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            9435..10355
                     /locus_tag="O2692_000389"
     CDS             9435..10355
                     /locus_tag="O2692_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4298037.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            complement(10463..10882)
                     /locus_tag="O2692_000390"
     CDS             complement(10463..10882)
                     /locus_tag="O2692_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298038.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(10879..11370)
                     /locus_tag="O2692_000391"
     CDS             complement(10879..11370)
                     /locus_tag="O2692_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCZ4298039.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQSTITWPEQLGHKIAIPHKG
                     ALK"
     gene            11467..12414
                     /gene="corA"
                     /locus_tag="O2692_000392"
     CDS             11467..12414
                     /gene="corA"
                     /locus_tag="O2692_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCZ4298040.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            12445..13494
                     /locus_tag="O2692_000393"
     CDS             12445..13494
                     /locus_tag="O2692_000393"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCZ4298041.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRNLNIHRG"
     gene            complement(13542..14750)
                     /gene="gltS"
                     /locus_tag="O2692_000394"
     CDS             complement(13542..14750)
                     /gene="gltS"
                     /locus_tag="O2692_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCZ4298042.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(15087..15695)
                     /locus_tag="O2692_000395"
     CDS             complement(15087..15695)
                     /locus_tag="O2692_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCZ4298043.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            16094..16741
                     /locus_tag="O2692_000396"
     CDS             16094..16741
                     /locus_tag="O2692_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCZ4298044.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSTIKNQPVTSETPEKACTSETPKTPEETDNQL"
     gene            16972..17871
                     /locus_tag="O2692_000397"
     CDS             16972..17871
                     /locus_tag="O2692_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298045.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLKLESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            17864..19096
                     /locus_tag="O2692_000398"
     CDS             17864..19096
                     /locus_tag="O2692_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4298046.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGITAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            19080..19265
                     /locus_tag="O2692_000399"
     CDS             19080..19265
                     /locus_tag="O2692_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298047.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            19314..19640
                     /locus_tag="O2692_000400"
     CDS             19314..19640
                     /locus_tag="O2692_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCZ4298048.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            19670..20689
                     /locus_tag="O2692_000401"
     CDS             19670..20689
                     /locus_tag="O2692_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCZ4298049.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(20925..21578)
                     /locus_tag="O2692_000402"
     CDS             complement(20925..21578)
                     /locus_tag="O2692_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCZ4298050.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            21752..22438
                     /locus_tag="O2692_000403"
     CDS             21752..22438
                     /locus_tag="O2692_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HCZ4298051.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(22504..23763)
                     /gene="sdpC"
                     /locus_tag="O2692_000404"
     CDS             complement(22504..23763)
                     /gene="sdpC"
                     /locus_tag="O2692_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HCZ4298052.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            24053..24988
                     /locus_tag="O2692_000405"
     CDS             24053..24988
                     /locus_tag="O2692_000405"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCZ4298053.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            complement(25244..25663)
                     /locus_tag="O2692_000406"
     CDS             complement(25244..25663)
                     /locus_tag="O2692_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCZ4298054.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(25785..26669)
                     /locus_tag="O2692_000407"
     CDS             complement(25785..26669)
                     /locus_tag="O2692_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ4298055.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            26829..28490
                     /gene="hutU"
                     /locus_tag="O2692_000408"
     CDS             26829..28490
                     /gene="hutU"
                     /locus_tag="O2692_000408"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HCZ4298056.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKFFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            28490..29728
                     /locus_tag="O2692_000409"
     CDS             28490..29728
                     /locus_tag="O2692_000409"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HCZ4298057.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTVSFKA"
     gene            30005..31126
                     /locus_tag="O2692_000410"
     CDS             30005..31126
                     /locus_tag="O2692_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HCZ4298058.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQSAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            31237..32118
                     /locus_tag="O2692_000411"
     CDS             31237..32118
                     /locus_tag="O2692_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCZ4298059.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            32605..33174
                     /locus_tag="O2692_000412"
     CDS             32605..33174
                     /locus_tag="O2692_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298060.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAIV
                     SNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     regulatory      33227..33322
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            33399..34712
                     /locus_tag="O2692_000413"
     CDS             33399..34712
                     /locus_tag="O2692_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HCZ4298061.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     gene            34879..35385
                     /locus_tag="O2692_000414"
     CDS             34879..35385
                     /locus_tag="O2692_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCZ4298062.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(35443..36207)
                     /locus_tag="O2692_000415"
     CDS             complement(35443..36207)
                     /locus_tag="O2692_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCZ4298063.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTKKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            36537..38141
                     /locus_tag="O2692_000416"
     CDS             36537..38141
                     /locus_tag="O2692_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCZ4298064.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASSGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            complement(38207..38737)
                     /locus_tag="O2692_000417"
     CDS             complement(38207..38737)
                     /locus_tag="O2692_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298065.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            39005..39922
                     /locus_tag="O2692_000418"
     CDS             39005..39922
                     /locus_tag="O2692_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HCZ4298066.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFVSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            39997..40632
                     /locus_tag="O2692_000419"
     CDS             39997..40632
                     /locus_tag="O2692_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ4298067.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            40643..40834
                     /locus_tag="O2692_000420"
     CDS             40643..40834
                     /locus_tag="O2692_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298068.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(40930..41115)
                     /locus_tag="O2692_000421"
     CDS             complement(40930..41115)
                     /locus_tag="O2692_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298069.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(41112..41468)
                     /locus_tag="O2692_000422"
     CDS             complement(41112..41468)
                     /locus_tag="O2692_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298070.1"
                     /translation="MLKQILLRATKISVLFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            41748..43127
                     /locus_tag="O2692_000423"
     CDS             41748..43127
                     /locus_tag="O2692_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ4298071.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            complement(43514..44386)
                     /locus_tag="O2692_000424"
     CDS             complement(43514..44386)
                     /locus_tag="O2692_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCZ4298072.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
     gene            complement(44831..45160)
                     /locus_tag="O2692_000425"
     CDS             complement(44831..45160)
                     /locus_tag="O2692_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298073.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELNKFKGFETSYIINENQVSYYEIITLLNKRPLRQVDYGNKIQYLNFYHTELSNAL
                     FAIKFAH"
     gene            45209..45946
                     /gene="sdpB"
                     /locus_tag="O2692_000426"
     CDS             45209..45946
                     /gene="sdpB"
                     /locus_tag="O2692_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HCZ4298074.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
BASE COUNT        15752 a         6384 c         8516 g        15332 t
ORIGIN      
        1 agggattaat gtcccagact ctttagttta tttattttca atataacaat tgtctaatca
       61 aggattaacg aatatttaaa gatagtttga cgcaatatta gaaacaacct ataataatag
      121 tttgtttgtg gattaactat tataaataaa agcggcgtaa agacatataa accaactact
      181 tgaacaatat aacgttaata acaatctata ctgatacatt acgcctagat aatctttgat
      241 gagcacatgt aagaaaaagt gatatggtgt atgacttccg acaccatcga tagataaacc
      301 taatttttgg gctagtcgta aggcgcgcaa tacatgaaac tgacttgtta cacaaacaat
      361 tttaactgct tcatgataca aattgttgat gatttgttta gaatataaaa agtttgtgta
      421 tgtatttata gagtgagatt ccattagtat atctgtttta tcaacaccat gtgcaatcaa
      481 ataacgttgc atagctaaag cttcagaaat tggttcgtct ggtccttgtc cgccagatac
      541 aatgatcttt gttgctgatg cttgttgttg atagatatca agtgcacgat ctaaacgcgc
      601 tgcaagcatt ggtgtgacaa attcggtaaa aataccagca cctaacacaa ttatgatatc
      661 aacttctttg ttgtatgatc tatgtctata tgatactgtc caaacgagat aacaaataaa
      721 ggttagtaac agggaaagac ataatatagc taaccacata gacaaacctt tcacaatagg
      781 tgactgaatc gtacttataa atagaagtgc tgatgtgtag agtacaaatt tatatgaaaa
      841 agataataat tttttaataa ataagcgact agaagtatga gaaaataaat atctatgttt
      901 gaatagcatg ataatactga ttattataaa tgttacaaac atagaccaag ggaaagtata
      961 ggtcatgatg ctatagatga gtgacaaaaa tatcgatatg acaactaaga tgtagcatgt
     1021 taaatttaac gtcagagtat agttgaaaat taacggacaa ataacgataa gtataaatat
     1081 taataataaa ttcaataaca tactgacacc tcgcttataa taaatattaa atataaatgt
     1141 agatgattta atttattaaa gcaaggagaa agcagcaaca tgtaaatctt aatttgttat
     1201 attatatatg ggtcaatatt tttgtgtttt ttagatgtgc gatacaatga ttgctgagga
     1261 tactttataa aaatagtaat tagggagaaa taattatggt aaaacattta caagaccata
     1321 ttcaattttt agagcagttt ataaataacg ttaacgcatt aactgcaaaa atgttgaaag
     1381 atttacaaaa tgaatatgaa atttcattag agcagtctaa cgtattaggt atgttaaata
     1441 aagaaccttt gacaattagt gaaatcacgc aaagacaagg tgtaaataag gccgcagtaa
     1501 gccgacgaat taaaaagtta atcgatgcta aattagttaa gttagataaa ccaaatttaa
     1561 atattgatca acgtttgaaa ttcataacct taactgacaa aggtagagca tatttgaaag
     1621 aacgtaatgc gattatgaca gatattgcgc aagatattac taatgattta aattctgaag
     1681 atattgaaaa tgtaaggcaa gtattagaag tcatcaatca tcgcattaaa acatatagca
     1741 atcataaata ggtttttgaa ggtgttcaaa tataagacta aatatgtatg tattgaacac
     1801 cttcttttta gttataattg agagagtact ttcataaata atgtaataag gtacaattct
     1861 aaagtaaagt taatcatttc ttgaattgta ttcttgggat aatatttgta aaataataat
     1921 ggtaaaataa tgaaataatt tttgtgatat acatataaca attgaagagg aacagaattg
     1981 aggtgaaagt atgaaatctt gcccgaagtg cggacaacaa gcacaagatg atgtacagat
     2041 atgtacacaa tgtggacata aatttgatag tcgtcaagca ttatatagaa agtcaaccga
     2101 tgaagatata caaactaata acatcaagat gagaaaaatg gtgccttggg caattggttt
     2161 tttcatttta atcttaatta ttatattgtt ctttttacta agaaacttca attcacctga
     2221 agcgcaaact aaaatattag tgaatgcgat tgagaataat gataaacaaa aagtagcaac
     2281 attattaagt actaaagata ataaagtaga ttctgaagaa gcaaaagtat acattaacta
     2341 tatcaaagat gaagttgggc ttaagcaatt tgtcagcgac cttaaaaata cggtacataa
     2401 attgaataag agtaagacca gcgtagcttc ttatattcaa accagatctg gtcaaaatat
     2461 attacgtgta agtaaaaatg gcacacgtta tatctttttc gataatatga gctttactgc
     2521 acctaccaag caaccaattg ttaaaccgaa agaaaaaaca aaatatgagt ttaaatctgg
     2581 tggtaagaaa aagatggtta tagctgaagc aaataaagtg acgccaatag gtaattttat
     2641 accggggaca tatagaattc cagctatgaa atcaactgag aacggtgatt ttgcaggcca
     2701 tttaaaattt gattttagac aaagtaattc tgaaacggta gatgttactg aagattttga
     2761 agaagcaaat atatctgtaa ctttaaaagg cgatacaaaa ttaaatgata gttctaaaaa
     2821 agtaactata aatgaccatg aaatggcatt ttcaagttcc aaaacgtatg gtccatatcc
     2881 acaaaataaa gatattacca tttcagcttc aggtaaagcg aaagataaaa catttacaac
     2941 acagacgaag acgattaaag ctagcgattt aaaatacaat acagagataa ctttgaattt
     3001 tgacagtgaa gatatcgaag actatgttga aaagaaagaa aaagaagaaa acagcttgaa
     3061 gaacaaattg atagaattct ttgctggata ttctttagcg aataatgctg cgtttaatca
     3121 gtcggatttt gattttgtat catcatatat aaaaaaagga tcatcttttt atgatgatgt
     3181 aaagaagcgt gtatctaaag gaagtttaat gatgattagt tcaccacaaa ttatagatgc
     3241 tgaaaaacat ggtgataaga ttaccgcaac cgtaagatta ataaatgaaa acggtaagca
     3301 agtagataaa gaatatgagc ttgaacaagg ctcgcaagac cgcttgcaat taatcaagac
     3361 atcagaaaaa tagagtataa aacttaaata agatgcatgt gtcatggaat acaaacaaca
     3421 tcctttcaaa gtatttacaa ttagtgtgaa ttactttgaa gggattgttt tttgtggaaa
     3481 aatttatgaa gtgctgaggt ttatatcatt ttataccttt tataagttgt aatgatgttc
     3541 atgaaacttg tgactaatgc taatatataa aagagcatat ttaaatttag tttctgttcg
     3601 tgatagttat aaactaatca ttatataaag gagatagtca tatgaatgat ttaacgcatt
     3661 ttaacaagcg ttcaccgata tttattatta ttttaggatc tttgacggcc attggagcat
     3721 tatcgataga tatgtttttg cctggattac cagatattag acatgatttt caaacaacga
     3781 cgtctaacgc acaattaaca ttatcgatgt ttatgattgg tttagcgttt ggtaatttgt
     3841 ttgctggtcc aatttcagat tcaactgggc gcagaaagcc gcttattata gctatgatta
     3901 tttttacatt agcaagttta ggtattgttt ttgtacataa tatttggctg atggttgcat
     3961 taagatttct tcaaggagtg acaggtggcg cagcagcagt tatttcaaga gctatagcaa
     4021 gtgatatgta tagtggtaat gaactaacta agtttatggc attattgatg cttgttaatg
     4081 gtattgcacc agttgttgcg ccaacaatag ggggcataat tttaaatcat tctgtctggc
     4141 gtatggtctt tgtcattctg actatctttg gcttcgtcat ggtcataggt tctttattaa
     4201 aagtacctga atcattaaca gtgacaaatc gtgagtcaag tagtggatta aagacaatgt
     4261 ttaaaaactt caaaatatta ttgaagacgc cacgttttgt attaccaatg ctcattcaag
     4321 gtatgacatt tgtaatatta tttacttata tttctgcgtc tccgtttatt attcaaaaaa
     4381 tttatggcat gactgcaata caatttagtt ggatgtttgc aggaattggt attacgctca
     4441 ttatatcaag tcaacttact gggtaccttg ttgattttat agactcacaa aaattgatga
     4501 gaggcatgac gatgattcag attatcggtg taattttagt gacgattgta ttgcttaatc
     4561 attggaactt ttggatttta gcgataggat ttatcatttt aatagcacca gtgacaggtg
     4621 ttgcgacact tggttttaca atagctatgg atgaaagtag tagtggtaga gggagttcat
     4681 caagtttatt aggtttagta cagtttttat ttggtggtgt tgcatcgcca ttagtggggg
     4741 taaaaggtga agataatcct ataccttaca taattattat tatagctaca gcagttatat
     4801 taattatttt acagatttat aatatgaagg tatttaaaac aaatcgatga taaacatagt
     4861 ttcttctatt atattgaatt gaaaaattaa aaaacaaaac ttgagctatt catagaccta
     4921 tgaatttagc atcaaaaata aaaatgccga gattattatg tggaaatctc ggcattttta
     4981 tattgatagc accgttacat atgtactatt atctatccat ttggtttaac tttatctagt
     5041 tgttttatag catttcttta ttagaatgaa accgtattat tagttttgaa gtttaatcgt
     5101 attagccaat aaaatgtcag caacttcttc aggtgattca ttacaaccat tcgaaagcca
     5161 attaataaac accccagctt gaccgcctat gatatatgta acataaagct gtttattggt
     5221 tactgtagta ctatagtcat tcataatttg ttgatagtaa tctctcgcta aatttatata
     5281 gtctatgaat aatgttttca ttggataggt aactaaaatg tctttgaaaa actgctcatt
     5341 gtgcttgata tacttagcaa taataatgaa gaatttataa agtttaagct tatcctgttt
     5401 aatattttcg aaattatgat gtagcgcgct tagaagaaat tggaattttt ccttatgctt
     5461 tgagttgatg gtatccaata aatcaaattt atctttgtaa taatcataaa aagtagaacg
     5521 attaatatta caatacgcgc aaatcatttt agtggatatt tcttgaaaag gatattcagc
     5581 catcaaatca acaagtgcgt tttgaatttc tattttggct tgtcgtttca tattgttaca
     5641 cctttgtgaa ttttaaccaa cactcttata aaaattgttg gtgttttttt agttatcatg
     5701 tctctataat aaataaagtg aattttaaaa gcaaatagga ggaaatgttt catgaaaaag
     5761 atgatattaa ttaatgtcat tacggttgtt gtactgttag caattgggat tgctggcttt
     5821 tatttttgga ataaaacaac aagctatgta acaactgata atgcaaaagt aaatggcgat
     5881 caaattaaaa tagcaagtcc tgcatctgga caaattaaat cacttaatgt taaacaaggt
     5941 gacaaactcg ataaaggtga caaagtagca attgttactg tacaaggtca agatggcgaa
     6001 acgaaagata tggatcttaa aatgcctcaa aaaggtacaa ttgccaaatt agatggtatg
     6061 gaaggttcaa tggtgcaagc tggtaaccca atcgcttatg catacaattt agatgattta
     6121 tatgtaacag caaatattga cgaaaaagat attaaagatg ttgaagtagg taaagatgtt
     6181 gatgtgacta ttgacggtca aaaagcttct attaaaggta aagttgatag tattggaaaa
     6241 gcaactgctg caagcttttc attaatgcca tcatcaaata gtgatggtaa ctacactaaa
     6301 gtttctcaag taatacctgt taaaatcact ctagaatcag aaccgtctaa acaagttgtt
     6361 cctggaatga acgctgaagt aaaaatccat aaaaattaag ggggtcatta aatgactacg
     6421 accttcatta ttagctacat tattttagcg ctcattattg ttggggttat caatttattt
     6481 ttaataagat caagaaaaaa aggcaaacgc caacaaaagg aacaacaatt tacgacacgt
     6541 caatcaaatc aatctaaatt taaagctagt gatttagaca aaacaactga tcaatcgaca
     6601 caacgtatga cgcatgaaga gttgcgtgtt gacaatcaag atgatcatag ccaagttagt
     6661 ctaaatggtt acacaaaggg gtctgagaaa gatcaagaag cattcactaa taataaggat
     6721 gaggaagcag ttgctgctaa aaatcctgaa tcagaagaat ataaagtgaa tgaaaaaata
     6781 aaaaaagaac ataaaaactt tatttttggt gaaggtgttt cacgcggtaa aatattagcg
     6841 gcattattat tcggtatgtt tattgcgatt ttaaaccaaa cattattaaa tgttgcttta
     6901 ccaaaaatta atacggaatt taatatttct gcgtcaacag ggcaatggtt gatgacggga
     6961 ttcatgttag taaacggtat tttaatacca attacggcgt atctatttaa taaatattca
     7021 tatcgaaaat tatttttagt tgcgttagta ctctttacaa ttggttcatt aatctgtgcg
     7081 atttcaatga atttcccaat catgatggtt ggacgtgtat tacaagccat tggtgcaggt
     7141 gtactaatgc cattaggttc aattgttatt attacaattt atccacctga aaaacgtggt
     7201 gctgccatgg gtacaatggg tatcgcaatg attttagcac ctgcaattgg tccaacatta
     7261 tcaggttata ttgtacaaaa ttaccattgg aatgtgatgt tttacggtat gttcattatc
     7321 gggattatcg ctattttaat tggttttgta tggtttaagt tatatcaata tacaacaaac
     7381 cctaaagctg atatcccagg tatcattttt agtacgattg gttttggtgc tttgttatat
     7441 ggtttctcag aagctggcaa caaaggttgg ggttcagtag agatagaaac aatgtttgcg
     7501 attggtatta tctttattat tctattcgtt attagagaat taagaatgaa atcaccaatg
     7561 ttgaatttag aagtattgaa attcccaaca tttacattaa caacaattat taatatggtt
     7621 gtaatgttaa gtttatatgg tggtatgatt ttattaccga tttatttaca aaatttacgc
     7681 ggattctcag cattagattc cggattgtta ttattacctg gttctctaat tatgggtcta
     7741 ctaggaccat ttgcaggtaa attgttagat actattggtt taaaaccatt agctatcttt
     7801 ggtattgctg taatgactta tgcaacatgg gaattaacta aattaaatat ggatacacca
     7861 tatatgacaa tcatgggtat ctatgtactt cgttcatttg gtatggcatt tataatgatg
     7921 ccaatggtaa ctgcagctat taatgcgtta ccgggacgac ttgcctctca tggtaatgct
     7981 ttcttaaata cgatgcgtca attagcaggc tctataggta cagcaatctt agttactgta
     8041 atgacaacac aaactacaca acacttatca gcttttgggg aagagttaga taaaacgaat
     8101 cctgttgtac aagatcatat gcatgaatta gcatcacaat atggcggaca agaaggcgca
     8161 atgaaagtgt tactacaatt tgtaaataaa ctagcaacgg ttgaaggtat taatgatgca
     8221 tttatagttg caacgatatt tagcatcatc gccttaattt tatgtttatt tttacaaagt
     8281 aataaaaaag caaaagctac agctcaaaag ttagatgcag ataatagtat caatcatgaa
     8341 taaataaaat aaattaattg aagtgtgact aatcaaaaat tatgttgtgg ggacatgatt
     8401 tttaaagtat cggtgccaaa tatggttatc gatacttttt ttatttgttg atttatagaa
     8461 tgttagagga attatattaa aatttggcat tgacgtagta ggtcattaat aaagaaaaag
     8521 caggaagtgg gtcaacgaaa tgaattttgt gaaaataaca tttctgtccc aatccctact
     8581 atataacatt attttaaacg aggcatgcga ttacggaaga ataagcttat aacaagtaaa
     8641 ccgatgctac agccaagtaa aatgatgccg ttatgaatag cgtcagcttc tgtaatcact
     8701 tgatctggtg gtacatttaa ataatatttt ttgaaaacat ctgcaatttc ttgtgcgagt
     8761 gctttaataa tataactgaa atacaatgta atgactaaca taattgttaa cactaaaata
     8821 tttactactt ttagaactgt gcgattaaat acaattgtca gtattgttat gactaggcta
     8881 acgatacata aaataaagaa accgttaaag actaaaatta agcgatcgat tccttgttgt
     8941 aaatcattga tattacccaa attaatattt aattgatgca tttttccaat tgtctcgtta
     9001 aaatttttaa tgccatcaaa atctacagat tctttggtga agacaatatt aaaaatgggt
     9061 tgtttccaca tgctgtatcc agcgattgcg gttaatataa caacaatgat ttgaataatc
     9121 atgcttacca atgaacgttt tttcttacca tatcgcttac cacgatgaat attcggttgt
     9181 tgaaatgtct catcaaatgg ttctaatcgt tgatttggat ttgtcatgca aggtacctcc
     9241 gtataaagtt cttttcttta cttatcaata tgtctagttg tctgttataa tgatgaaaaa
     9301 tcactgttca attgtagtct taaatttgaa agtgaaaaag aaaatatctt ctacattata
     9361 tacttttttt ggcaaagaat gtaggggata gacaatcatt ggatacataa aatatataaa
     9421 ggtaggttaa gacattgatt agaaaccgtg ttatgaatag cgttgtcaat aaatatttgc
     9481 ttcacaatcg ctcgattatg tttaaaaatg atcaagatgt tgaaagattt ttttataaaa
     9541 gagaaattga aaatagaaaa aaacataagc agccttcaac attaaatgtt aaagcaaatt
     9601 tagaaaaatt gtcattagat gatatgcaag tctttcgctt taatttcaga catcaaattg
     9661 ataagaaaat tttatatatt cacggtggat tcaatgcact acaaccatca ccgttccatt
     9721 ggagattgtt ggataaaatc actttaagta cattatatga ggttgtactg cctatctatc
     9781 caaagacacc agagtttcat attgacgata ctttccaagc gatacaacgt gtttatgatc
     9841 aattagtatc tgaagtagga catcaaaatg tcgtagtcat gggtgatggt tcaggtggtg
     9901 cactggcatt atcctttgta caatctcttt tagataatca acagccatta ccgaataaat
     9961 tgtatttaat ctcaccaatt ttagatgcaa cactatctaa taaagatatt tcggacgcct
    10021 taatcgaaca agatgcggtt ctaagtcagt ttggtgtcaa tgagattatg aaaaaatggg
    10081 cgaatggcct accattaaca gataagcgca tatcgccaat taatggcaca atagaaggat
    10141 tgccaccagt ttatatgttt ggtggtggac gtgaaatgac acatcctgat atgaagctat
    10201 ttgaacaaat gatgttgcaa catcatcaat acattgagtt ttatgattat cctaagatgg
    10261 tacatgattt tccaatttat ccaattagac aatcacataa agctattaaa caaattgcca
    10321 aatcgataga tgaggatgta acacaaaata actaataaca tataaatgac ccagttacga
    10381 tttcatgcaa gatgatattt gacatgaaaa cgtaattggg ttttgttttt gaaaatatag
    10441 caagggttat atagactaat tgttaatgag cttctgagtg aaaatcacca ataagtgtat
    10501 tgatatgtgt gatgataaag tattgccctt taatttgata aaattgatct aaaaaattat
    10561 cgaattgacg tggaatatca ctaagttctg caaagttagc tgttttatca taattgcgat
    10621 gagcaacctc attttgaaat ttatcataat gttctaggaa atgttgatct tcaagctgtt
    10681 gttccacaat agtcataata ttatcgatgg cttgcttttg tccttgaaca taagccatat
    10741 gcacgccacg cttaagtagc tgatttgctg gtccgttaat gtataaccct ttttgtgcat
    10801 ttaattctgt taattcaaac ttaacatcat ctaataaatc tagtaattcg atatgaatct
    10861 tttgttgtaa tgcatctgtc attttaatgc tcctttgtgt ggaattgcta ttttatgtcc
    10921 taattgttca ggccaagtga tagttgattg atttttataa tagtgtgcta tttgtgttga
    10981 aaatgattca ggaaaagcat cagaacgacg tgtgtgctga ttaattccca tcatcattac
    11041 ttcatttgtt gatgctagtg taccatcttc tttagttaat gttaaaaata aatgcaaccg
    11101 cttataatcg taatcataaa tatataaagt aacagtaaat acatcgccaa gagacaattc
    11161 tgagaggtat gtcgtatgtt cttctagtgt aaatagcgta tatgctaaat tttcgcgttc
    11221 ttttaaagaa agaccgtggc tgtaattaaa acgattcacg acgtcactaa aaatgatatt
    11281 ataatttgca tcatgcatat gattgttatg gtcaatgaat tcagatgtta cggtttgagt
    11341 atgtgtaaaa agttgtttga taataaccat ctccttagtg actatcatac taaaataaat
    11401 taagacatac acgttattta attcatgata aaataataga gaattcgtta tttaggggga
    11461 gtatcgatgg ctttaaagat acggtatcaa actacatatg aaccgtttaa agtagttgat
    11521 gatatcaaag agataccaaa agatgcgact attgtttggt atgattttga tgaaccgaat
    11581 gaacaagaaa atgaatggtt taaagcacat tttaatttta atgatttaga agtggatgat
    11641 gcaattaacg gtatgccacg agctaaatat aaatcttata aagattatca atacttagta
    11701 ttccatagta ttatggggtc aaactattca ccaatagcac taaatatttt tatacaagac
    11761 aatgtattag taacatatca tcatcaaacg ttggaatcat taaataaagt agtctataag
    11821 tatatgaata cattagatgc agaattagat tgtgcagatg tcgtcatatt aattttagat
    11881 atgatggttg ataaatattt caactttgta tatgcacttg aagatagtgt ttatcatttt
    11941 gaagaccgcc atgtagatga tcgctttaat aaaatggtca tggatagtgt ctttaagtta
    12001 cgttcggatt tgattaaagt aaaacgtgta ctgtttccta tgcaagagct aattgataca
    12061 atgaaacaaa atggtgattt gattatagat aataaacatt cgctatacat tcaacatatc
    12121 gatgaccact tgattaagca acgtaatatc attcgtactg ctcaagaaat gacaaacgaa
    12181 atacgagaaa attttgagtc atacacgtca tttagaatga atagtattat gcaagtactg
    12241 acattagtat cagttatatt ttcaccgtta acattcatag ctgggattta tggtatgaac
    12301 tttgtaaata tgccggcatt acatttacat tatggatatt atatttgctt agctgtaatg
    12361 tttgttatag ctgtcgtatt aatcatcttt ttcagacgta aaaaatggtt ttaaattaga
    12421 aatgacgaaa aggtaggttt aatcatgagt gattttcaaa gagaacagag aaaaaatgaa
    12481 catgttgaaa tagcaatggc ccaatctgac gcaatgcatt cagattttga taagatgcgt
    12541 tttgtgcatc attctatccc atcaattaat gtaaatgata tcgatttgac atcacagacg
    12601 cctgacttaa cgatggcata tccggtttat attaatgcaa tgacgggtgg tagcgagtgg
    12661 acgaaaaaca tcaatgaaaa gctagctgta gttgcaagag aaactggctt agcgatggca
    12721 gttggatcaa cacatgcggc attgagaaat ccacgcatgg ctgagacgtt tacgattgcg
    12781 cgaaaaatga atcctgaagg catgattttt agcaatgttg gtgcggacgt accagtagaa
    12841 aaggctttgg aagcagttga attacttgag gcacaagcgt tacaaatcca tgttaattct
    12901 cctcaagaat tagttatgcc tgaagggaat cgtgaatttg tgacttggtt agataatata
    12961 gcgtcgattg tatcacgagt gtctgttcca gtcattataa aagaagttgg atttggtatg
    13021 agcaaagaat taatgcatga cttacaacaa ataggcgtca agtatgtcga tgttagtggc
    13081 aaaggtggta ctaactttgt agatattgaa aatgaacgtc gtgcaaataa agatatggat
    13141 tacttatcat catggggaca gtctacagtt gagtcattac ttgaaacaac ggcttatcaa
    13201 agcgaaattt cagttttcgc gagtggtggt ttacgtacac cactcgatgc aattaaaagt
    13261 ttagcacttg gtgcaaaggc aactggtatg tcacgtccgt ttttaaatca agttgaaaat
    13321 aatggcattg cacatacagt agcttatgta gaatcattta ttgaacacat gaaatcaata
    13381 atgacgatgt tagatgctaa aaatattgac gacttaacac aaaaacaaat cgtatttagt
    13441 ccggaaatat tgtcatggat agaacaacgt aacttgaata tacatcgagg ataaataaaa
    13501 gaggaggcga aggcctcctc ttttatttat gagtttggtg tttaactaaa ccattgtatg
    13561 aatcccataa tgactataac accaattaaa tcgattaaga atgcaccaac aataggtaca
    13621 actaaatatg ctttaggtga gtttccatat tttttagtaa taacatctaa atttgccatg
    13681 gcatttggcg ttgcaccaag cccatgaccg ataaaaccac ctaccattac tgcagcatca
    13741 taatcttttc ctaaacctct aaataaaatt aaaacagcaa ataaaatcat aacgacaact
    13801 tgaactaaaa cgataataat aagaggtata gcaagtttat aaatttcgat taattgaatg
    13861 ctcattagcg caagagataa gaaaatacct aatgcgacat cgccaatttg attagtaatt
    13921 tttaaatcta caatattaaa attaaaactt tcagagatat ttcggacaat aacagctaca
    13981 aataatgagc caacgtaaat tggaacatta atccctgttt gagctgtaaa caaatgactg
    14041 aaataacttc caactgccat acagaataca acgattgtaa attgaatgaa gaatacttca
    14101 gtggcattaa atttactatg taaatgttcg ttatatgcta cttggctata atctttaaat
    14161 gtatcgtcac tatgttgagg ctttaagtta taacgtttga ttaaaaattt tacaactgga
    14221 ccaccgataa gccctccaaa tacaagacct aaagttgcag ctgcaagagc cgctgtcagt
    14281 gccgaatcaa tacctaaatc ttgaattgtc ttaccataag cagcagcatt accatgaccg
    14341 ccttccatag acatggaacc tgctgttaat cctaacaaag gtttaatatt taatactttt
    14401 gctagtgata caccaactat gttttgaatg actgaaatga tagcacaaaa cataaagtat
    14461 aatagcaaga ctttgccacc taatttaaat aatttcaatg atgcaccaag accgattgtc
    14521 gtaaagaatg ctaacatgaa gaaatcttga atgaatgaag catctaattt aatcttaacc
    14581 atgccaaatg aatccaaagc cgcaactaaa atagcaaaga ttaagccgcc aatcactggt
    14641 gctggtatac aaatacgttt taaaaaatta acgtgattaa cgatagcctt accaagtaaa
    14701 taaaggatac aagctaaaca taatgttgta attgcattaa gttctatcat tttcaaatcc
    14761 cccttgttga atatccctct ccctaaataa gcttttatat gtaagcgtta taaaagggcg
    14821 cttttaacat tatacatgca acaaatcaat atttctagtt tttgaaaata atatcaataa
    14881 tattaatgca tctaaattta ataattataa ataattctat tgttatattt tataattaag
    14941 aacaagttca ataagaggag atgaatcaaa aaatatgatg gatacaaaga aaaacaatca
    15001 acccatccgc atgttgacgg cacgattgat tgacttataa ggagatagat ttcattttca
    15061 tgatagtaat caatgaaaga tggcatttat ttccaagtat cttcgggaaa catacaatct
    15121 gatttacgca tgcgagacac aaatggatta cctttcactg tgtaacgtaa agatttatgt
    15181 gtccaatcac ctttatttgg aataccgatt cgtggactag caataatatc tttaggatat
    15241 ttacgattct tagtatcaat agacaatcta cagtcattta acgtagcgcc atcgatagcc
    15301 cgtggaatgt taaatgcctt agtccatttt cctgggccat tcgttacctc gtagcctttc
    15361 ttacctctgt tacggaacat agcggataaa ccttcttctg gttcaattgc gcggataagt
    15421 acgccttcag gtataccttc agattttgtt acaaaattaa tgagtaaatg cgtatgcatg
    15481 acatgtgcat aaattgtacc accacgttta tataatgacg tgactttagg tgttatttta
    15541 ccgccatagc catgagccgc acgatcattc aaacctaagt aagcttccgt ttccacgatg
    15601 tagcctgtat acgtttgagt ggtatcctga taaatcactt tgacacctaa taagttttta
    15661 gcaatttgtc ttgtatcatt attaacgaaa tccataatat tctcctcgca attatatgat
    15721 taatttaaat aaaaatgaaa aagatatttt tattgtggta aatgtttgat aaaatagtat
    15781 tgttaagaat tataaaaatg taaagtatat actttgttat ggaatatttt atttgcatag
    15841 attgagactc ttttaaacgt gaacattgca gtatgacata tttttagtat agttaacgaa
    15901 gcgttgactt tcaaatgagt ctccatatag atgtgtactt gtagcgggtt ttcattttac
    15961 tagtgatgaa atataaagtt cattttccaa agtgattgac gattgaaatg aagtggcttg
    16021 ataatgaatc ttgtaaatta taacggtaac aataattaaa aatataaata tgattaacga
    16081 aagggagatt acaatggaac aattacctca agtaggtttt atgaaatcat tcgttttatt
    16141 ttggaaaaat tatgtaaatt ttaaggggag atcaagacgt agtgaatatt ggtatatggc
    16201 actatggcat ttgatactta tggttccagc tgtgtgtgtg ctaataataa acctatttct
    16261 attctttatt tctatagcgg cggataacga aacattaata gttatgagta tattgatgat
    16321 tatccttaca tctgtgtata cttttgtcta tagtttggca acgtttattc caaatttagc
    16381 gttgtgcgtt cgaagatttc acgatatatc aagaacaatg ttatttccaa tgattatgac
    16441 agtattcagt attatatttt atatagtatt acagattatc gatagttatt atgacagtga
    16501 ttttacatta atgccgatgg gtcttaatat tttgttagta cttctttatt ttgtttattt
    16561 aggcctaaca attgttatga tagtattcct atgctttgac agtaagccgg caaataaata
    16621 tggcgaaagc ccaaaatacc cgtctacaat taaaaatcaa ccagtgacat ctgaaacacc
    16681 ggaaaaagcg tgtacatctg aaacaccgaa aacaccggaa gaaacagata atcaattgta
    16741 atgatataaa tatctcggca caatgatgct gggatatttt tttacaaata aggcaataaa
    16801 ggcatggaaa aaacatatct agtgctaatg atacttaaca tgcaaaaatg gcactattta
    16861 aatttttcaa tttcagaaat tttaaaaata aattcatttt aaaaatattg catttataga
    16921 gaatggaaat gttatattaa gataaataca tattttttta ggaggcaaag tatgggactt
    16981 gtaattgaac atgttacgaa acgttttggc aagatgacgg cagtaaatga tatttcatta
    17041 aagttagaat caggtaaaat gttaggtttt ctaggaagaa atggtgcagg taaaacaacg
    17101 acatttcgta tgattttagg cttaagtgaa ccaactgaag gacacattac gtataacggt
    17161 aaaaaactag ataaaacaat gtacaatcgt attggttatt taccggaaga acgcggttta
    17221 catgggaaat tgacagttga agaagaattg aaatatttgg caactttaaa gggaatgtca
    17281 aaaacagaaa tacaacagca aatatcgtat tggcttgagc gttttgatat tactgaaaac
    17341 cgcaaaaaac gaattgatag tctatcaaaa gggaatcaac aaaaaattca gctgttagca
    17401 agtatgttac ataaaccaga attactaata ttagatgagc ctttcagtgg tctagaccca
    17461 gttaatgttg agttattaaa agaagcagtt aaagacttaa atgattgggg cagtacaatc
    17521 gtttatagtt ctcaccgtat ggagcatgtt gaagaattat gtgatgatgt ttgtatttta
    17581 gataaaggtc aacttgttgt ttctggtgat atcaatcatg tacgtgcatc taatggtaac
    17641 aagaaagttg tcattgaatc agaaacaaca ctaccagatt taacaaacat tagaggcatc
    17701 attcatagtg aaaacatgaa gcaaggtctt cagttgacga ttgagaatga agacgtagcc
    17761 aaagatattt atcaagttgt ggcacatcaa ggttatgtta aacgattcca agttgtagaa
    17821 ccatcattac aagatatatt cattgagaag gttggtggca aggatgcata agttttgggc
    17881 gacatttact ttgacatata tcaaaaaaat taaagctaaa tcatttgtaa tttttatgat
    17941 tattattgct gccttaatga ttggcctatc taacattgat aagattatta acatgtttga
    18001 tgatggtcca gataaaattg gtgtagcagc accgaatgaa caaatctata aggttttcaa
    18061 gcaacaggcg aatacatttc atagtgatgc taagtttact aaagtttcta tagaagatgc
    18121 tgaaaaagaa gtaaaaaaac ataaacttga taaagcttat attatcaagg tgaatcaaaa
    18181 tcgcacgtta caagggacaa tcattagtga gaagcgagta agtcatgaag attcacaaaa
    18241 agtacaagca ctgcttacag ctattcaaac aaatatggta gccggggaat taaatatcaa
    18301 caaagaagac ttacaaaaat tacaagcaca aagtaaagtt gataataagg tgatttctaa
    18361 tgatgaagtc gataaagtat cagaaggaca aaagattttc aactatgctt tagcttatgg
    18421 cattatcttt ctaatgttct tcattgtttt aaattatgca tctcaaatag ctatggaaat
    18481 tgctagtgag aaaacatcgc gtgtcattga aatgattatt acaagtattt caccaatcca
    18541 acatattttt gctaaaattt taggcgttat agcagttgcc gtgactcaga ttattttaat
    18601 tgtattgatg gcagttatat gcatttttgc ctttgactta aaagacttgc tacaaggttt
    18661 taacgtggaa atgaatcaat tgtcttggca aattattatt gtcggaatta taagtagtat
    18721 tgttggaatt acggcgtatg tattacttgc agccatcctc ggatcattaa catctagaat
    18781 agaagattta aatcaatctt tgatgccact gacattactt ggtatgattg cattttatat
    18841 cgcgatattc acgataaata atcctgatgg acaacttgct ttaatcacaa gttatattcc
    18901 gtttttagcg ccatttcaat tagttgtacg cgctcaaact tcagggcttc aaatatatga
    18961 ggtcatctta agttctttaa tctcaattgt aatggtagct gtcttgatat ggattgcaat
    19021 taaaacatat aaagattcag tacttacatt tgaacgtggc ttatttaatt caatgaaacg
    19081 tgtatttaaa aaataaaatt gatgagagtg acacacattt atcatataaa atgatgatga
    19141 aagcgtgtta ctcttatttt tttatattta gctatagaat aatgtctaaa aaagtgtaca
    19201 atatgaatgt gaaaacgaac tttttaaaat gggaatgtga agtaagaaga aatgtaaaca
    19261 aatgatatgt agccgataga ttattgatag caaatgtgtg atggagtgat ttcatgttat
    19321 atccgatttt tatatttata ttagctggct tatgtgaaat aggtggggga tacctgattt
    19381 ggctgtggct tagggaagga cagtcttcac ttgttgggct aataggcggt gcgatactca
    19441 tgttatatgg tgtcattgcg acatttcaat catttccatc attcggaaga gtatatgcag
    19501 catatggtgg cgtatttatc atcatgagtt tgatttttgc gatggtcgta gataaacaaa
    19561 tgccagataa atatgatgtt atcggtgcaa tcatttgtat tgtaggtgtt ttagtaatgt
    19621 tgctacccag cagagcttga acacttgtgg ttagaaggga gaagtaaaaa tgatagttga
    19681 agttgagcac caaagacaag gtgtagattt aatcaaaatt gataatgatg aaactaaaat
    19741 tatttttacg aattatggag caagagtagt gtcttggaaa tatcatgata ataatattgt
    19801 tcttggaaat gttgttgaag cggatgaatt ttatcatagt aatcctttta aatttggtgc
    19861 ttctatagga agatatagcg gtagaattga taatgctaaa tttaagttga aaggtaaaga
    19921 atatcagtta gaaaaaaaca acggtgaaca tcatttacat ggtggatgtc atggtttgga
    19981 caataagtta tttgattatg aaattagaaa tgaaattgct caaattaaag ttatatttaa
    20041 gacggtgttg aaatcagctg atgatcattt cccgggtgat atcgatgtga cgattacaca
    20101 tatttatgat gcggatcacc aatggtcgat tgaatacgaa gcagttgcat cagaagacac
    20161 attgtttagt cctacgaatc atgtgtactt caatttgaat cgagataata atgtcgttga
    20221 taatcatcgc atttcaagta atcaattaga tatgtatgta ttagatgagc gaaatattgt
    20281 tacaggtgat attctagatt tacatgaagt gtttgaagat aacaagataa aattgagtga
    20341 tatttttaca tcacaacatg cgcaattaag ccaacaaatg acacgttttg gtggtctcga
    20401 tcacccattt acagttggtg aacataaaat gtatgttgaa aatcatgaat ttatgttaga
    20461 ggtcgataca gatatgcctc atgttgtgtt ctttactttt aaccagcctg atgagtggga
    20521 tagtccattt aatatttata aaccacattc tggctttaca ttagaaacac aatttttacc
    20581 aaatgatatt aatttatatg gtgaaaaagc accgtcaatt ttaaaagctg gtgaagtgtt
    20641 caattcgaaa acaagtttcc gtatcgaaga aaaagtagaa aatgattaaa agtatcggca
    20701 tttatataat atttatgtta tttggcggaa ttttgaatag gttatcaaga atcgtatcaa
    20761 atgaagtgac atcattaata tacagtttga aataaagaaa atatataaca taaaaaagga
    20821 atcaatagca tcagtggtta tagataagac tattatacaa acgatgtgta agttgattcc
    20881 tttttgtttt gaaaagttca tttaacactt ataatgaaag gtaattattt caatgttctg
    20941 ttatacattt tttgaagttt atcgcgtctt gttggtgaca gaaatggatt gtttagtgca
    21001 tatcgtaaca tatcttgatt ttgtctatca ttataataaa ggtcattaag ctgttgtaca
    21061 gataaacgat gttcgattgg tgcttcttgt attaagtaaa gctgatcatt tggagataca
    21121 tagccttgtt ttaatacccg gaaatagaaa cctgttttac cagatgtaga catgcgcttc
    21181 actaaatcag gaatattata ttttgcttga attttccagc atggttctct aatttcagaa
    21241 acctcaatta acgcttcacc taaacgatat tgattaccaa agtaaacatc agattcgtct
    21301 aaataatcaa atgttaaatt ctctccaaac atcgcatgag taggtagtgt aggtaaatca
    21361 tcttgataca gtgcataatt atgcttacta aacccacaaa ctgctttatg tggtccacca
    21421 tgatctttat aagcctgttc atcaccagtg aaaccagttg aagataacca tgttgtttgt
    21481 gaaatcttag ttttatctaa agcagagcgc atcggtcttt tcgagctata aggcaaatct
    21541 tggatttttc ctgtcgaaat tgcgtgaatt gatatcatat cattctcctt aacgagtatt
    21601 atttaaaata tattgtacta taaaacattt ttagaattgt aaattctgat tgatatgttt
    21661 aaaaaaatgt tagaaataag ggtatgttaa tttatacgag tcatgtaaaa tatgaattga
    21721 atagattaca taaatatggg aggctacctt catgaaagat gtcaaagcac tcaagttaat
    21781 gacattaaat gatgttttaa gtcaaatcaa tggtgacatg acgctgggaa ttggtacagg
    21841 aagtacaatg gaattactct tacctcaaat ggcgcaacta attaaagaac gtggttacaa
    21901 tattacaggt gtatgcactt ctaataaaat tgcattttta gcaaaagaat taggtattaa
    21961 aatatgtgaa atcaatgatg ttgatcatat cgacttagca attgatggtg ctgatgaagt
    22021 agatccatct ttaaatataa ttaaaggtgg cggtggtgcg ctgttcagag agaaagttat
    22081 agatgaaatg gcatcgcgtt ttgttgtggt tgtcgatgaa acgaaaatag ttcaatactt
    22141 aggtgagacg tttaagttac cagtagaggt ggataaattt aactggtatc acatattacg
    22201 taaaattgaa tcatacgctg atataaaagt agaacgtcgt gtaaacgaag atgtcgcatt
    22261 tataactgat aatggcaatt atattttaga ttgtaagtta ccaaaaggaa ttgacccata
    22321 taagttccat gagtatttaa ttcatctgac aggtgtgttt gagacaggat acttcttaga
    22381 tatggcagat caagttattg taggcactca agagggtgtt aaaattttag aaaaataata
    22441 tatagtatag aaaagcgctt gctagcatca tctttttaag aggtactagc aagcgctttg
    22501 ttattatttg tttttatctg aagattgttc ttcagttttt tcgatatgtt tttctgcatc
    22561 aattacaact gatgaacgtc tgtcttcttt ataagtcgca ggttctttta atgattgctc
    22621 tatttcatct tcatttgaat cgtttgttaa atcgtcactt tgtggtgctt gtttaactga
    22681 ttctgctgat tctactgact ccgaagtgat gtcatgatct tcatcatgat tagattcagt
    22741 ttgattacct atgtgatgtt gaggttctgt tttttcagta ccttcatgaa tgcttggttc
    22801 gtcagccact gtattttcag tatcattttt agcaacacca accgttgatg cagtagctac
    22861 acctgttttt tcagcatctt taatatttga agaagatttt tcagcctcag tatgattagt
    22921 ttcttcatcg tcattgacat ggtctaaata attattaggc tcaagttctt caaggtttcg
    22981 tcttgttgtt aaatatgcaa tacctcggat aattaagctt aaaccaatgt atcctactgc
    23041 aacgattgct gttgaaatgg cgatgacttt gattgaaaat agatcgccga tttcttcgct
    23101 aaacaagaaa ataagtccga atgtcattga agcatggaat gtcgttgcaa tataaattgt
    23161 acgtccttta gtcgctctaa ttaattcacc aagaatcata gagaatgaga atgtataaag
    23221 gaagttataa gcagcaaatt ctgtaccata agttgtattt gctgagaaaa ctgaatacat
    23281 caaaccaaca acaatacttg caaagaacgt gttcattttc gtttcaacaa tattttgtaa
    23341 gtatgaacgg aatccgaatt ctactacgaa cgccatcaga atatgtccaa tcagaatgtg
    23401 tgtaataggt actgataagc ctgttgattg taataaaata aagctatctg caaatgtatt
    23461 aaagctgtac ataccaataa ttagaataat taaaggtaaa attaaagcta gcaatagacg
    23521 ttcaattact ttcaggctga ttgagaattt taaacctgca agttggacct ttttatattt
    23581 gaaaacgagt atacaaatga ttgcagcaat taatggtgct agatctgtaa cttcaaatat
    23641 aaagtgtttg acaccaatta tagactggaa atctctgagc ataatggata acgccattgt
    23701 aatgacaaag aagacgaaaa tcgtcattgc ccattgaaaa ccagaaattt tattgttctt
    23761 cattatatgt aacctccatt aggtaacaag caaaatatct tttagtaaca attatacata
    23821 ttacaagccg agagataaat tgtctatgtc aatattaatg aaaatgtaat agtcttgacg
    23881 tattttatta acataaatca gctaagtatt cgttattaca tagaaaatag gggtaaaagt
    23941 aatgtagata agtattttta agatacggat ttaactatga attgaattca aaatttccta
    24001 tacgttatac tttaattgtt aataagccac ataaataaga gggggaaatg ctgtgtacaa
    24061 gcaaggtgaa ccaaatttat ggactggaag gttagatagt gaaacagacc cgaaaaaatt
    24121 tagacatttt caaacagtaa catttgaaga tttgtctaag ctggaaaaga gtagtatgcc
    24181 atcaggggtc ggtatattag gctatgctgt tgacaaaggt gttgctttaa acaaggggcg
    24241 cattggtgca aaagaaggac cagatgcgat taaacaagca tttgcaggtt tgccggattt
    24301 gaatcaatgt gaaactttag tcgattacgg aaatgtttat catgatcatg aggaattaat
    24361 tgatactcaa aaagaatttg ctatgcttgc agcgaagtca attgctaatc atagacaaac
    24421 atttttatta ggtggtggac atgatattgc gtatgctcaa tatttagcaa cacgtaaagt
    24481 ctatccaaca caatctattg gtgtgataaa tattgatgcg cattttgata cacgtgctga
    24541 acaacaatct acatctggaa cgagctttag acaaatttta gaagaagatg aaaacacaga
    24601 ttatttagtg cttggtattg ctcaaggtgg taatacgcaa agtttatttg attatgctaa
    24661 agagaaaaag attgattatg tctttgcaga tgaattattg agtcacgtat caccaacaat
    24721 taaagatatg attgaacgtt ttgtacatga acatgatgtc attatgttta cgatttgtat
    24781 ggatgtcatt gatagtgcgt ttgcgcctgg ggtaagtgcg ccggcagtgt taggtttgta
    24841 tccacatact gttcttgaat tagcaaaacg tattattcca agtgataagg tgtcttcagt
    24901 tagtattgct gagatgaatc caacatatga cgctgacaat agaactgcta agctcgttgc
    24961 taatttagtg catcattttt taaaataatt aaaaatattt atatgtgacg agatttgcat
    25021 tgcagggcag aatgtgagta tttcttaata gaagttatat taacgttgat atatttgaaa
    25081 aataattgca gtaagaagac tcttaagttg tagtcatctt actgcttatt tatgtttgtt
    25141 gaaatagcaa aaataacaac tgtattgttt gaaaagttct cttattatat aataattaat
    25201 taaaatttta acggagttca agaggccttt ttataatgac accttatttg taaaatgtca
    25261 tatgtggttt agcctcttta taataattta atctgttctc aagtgtgcca gtatgtaatt
    25321 ctagcttatg accatcaggg tcggtaaagt aaattgattg tctatctcta atatctctaa
    25381 ctcttccttc taaaatattc acgttattat cttttaacct ctgatgccaa tatttaaatt
    25441 cgctgtcatc tatagtgaaa gctatatgtg tatatgaaaa gtgaatttca ttacgtggta
    25501 tatctttttc ttcatttaaa gcaatccata ggcctgcaag ctcaaaataa gcagtttttt
    25561 taccagtcaa tagcaatttc ccaagtaaaa tatctctata aaaatgtatt gaatcgttta
    25621 aatttctgac tgaaaagcat atatgattaa tagattttaa catctgcttc acttctttct
    25681 tagaattttt ataataaatt atttttgtat cgatttttga aatgacgtca ttaattttta
    25741 tacgatgatc tgcgcaactg cataagaggc tctaataact taagttaaag agcctctaaa
    25801 agtccatata aataatcttg gcattgttgt acgaatgtat gtacttgttc ggagtgtttg
    25861 cgttttttat ggtaattgat ataaattttt cggcctaagt ttggacgaat ttttttatat
    25921 tctaaattag acgtgtgaaa tgattggtaa taaaatctcg gaatgatagc gtaacctaag
    25981 ccgagatgaa caaatccaac tgctgattcg aatcgatctg tttctacaac gacatttgga
    26041 cgaatatttc ttctattaaa atagtcatct aagtgtttgc gcacttgaga atttttgttt
    26101 ggtaatatga gtggcaaatt ttcaacatct acccaatttt gatttttaaa tgtttccttg
    26161 ggtgctaata aaatgtaaga ttcctcatat aaaggaatgg atcttatatc ttcgtgggtt
    26221 attttttcat ttgttatagc taaatgaata ttaaaattca gtaattgctc tatagattga
    26281 tgtttatcat gtatttcata taaacgatat tgctgttcag ggtagtcgga atggtgcttt
    26341 cgaattaaat tcgcaatcca ttgattcgta gattcaagag tcccaatttt tatccttggt
    26401 tctgatgtaa cgcttaaatc atacattttt tccatcgtgg atcgatattg ttgaactaat
    26461 tcgctcgcat aacgataaaa ctgtattcct ttttcggtaa tcttgatgtc ttttgttgaa
    26521 cgtgtaaata agtcataacc taaatctgct tccatttttt taatcgtagc agttaaagat
    26581 ggctggctaa tatgtaaaaa ttgtgcagct ttagtaaaac tattatattt cacgatagcc
    26641 aagaagtatt ctaactgaat aattttcatg tgttttgctc cttttattta tagattcaag
    26701 ctatggataa ttagtttatt tatatttgtt ggctatattt taacatacta taatgaatat
    26761 tgaagataat aatgtaagcg ttttaaattt agtggaatta acttcttaaa agaacaggag
    26821 cgaattttat gagaaaaatt caagcaaaaa aaggtttgag tattgagtgt aaaggttggg
    26881 aacaagaagc agtacttaga atgttataca acaatttaga cccagaagtt gctgaaagac
    26941 cagaagattt agttgtttat ggtggtattg gaaaagcagc acgtaattgg gaagcttttg
    27001 aagcaataga gaaaacgtta cgagaattag aatcagatga gacaatgtta gtacaatcag
    27061 gaaagccagt tgctgtattt aaaacacatg aagaagcgcc acgtgtatta atttcaaatt
    27121 cagtattagt gcctgaatgg gcaaactggg atcattttaa tgaattagat aaaaaaggtt
    27181 taatcatgta tggacagatg acagccggta gttggatata tattggttct caaggtattg
    27241 tgcaaggtac ttatgagaca tttgccgagc taggtaatca acactttaat ggagatttag
    27301 caggcactgt tacactgaca gcaggtttag gtggtatggg tggtgcgcaa ccgttagcaa
    27361 ttactatgaa tcatggggta gcaatttgcg tggatgttga tgaaacacgt gttgataagc
    27421 gaattgatac gaaatactgt gatgttaaaa cagctgattt agatgaagca ttaaaattag
    27481 cagaagaggc gaaagaacgt ggagaaggat tatcaatcgg gttagttgga aatgctgtag
    27541 acatccatca agcgattcta gaaaaaggat ttaaaattga cattattact gaccaaacaa
    27601 gtgcacatga tccgctaaat ggatatgtgc cacaaggata ctctgtagaa gaagcgaaag
    27661 tattgcgtga aaaagatccg aaaaaatatg ttgaactgtc acaagcttca atggcaaagc
    27721 atgttgaatt aatgcttgaa ttccaaaaac gtggcgctgt agcatttgat tatggtaaca
    27781 atattcgtca agtagccttc aataacggag tgaaaaatgc ttttgacttc ccaggctttg
    27841 taccagctta cattagacca ttattctgtg aaggtaaagg gccattccgc tttgctgcgt
    27901 tgagtggtga tccaaaagat atcgagcgtg cggatgaaga aatgcgcaaa tttttcccag
    27961 aaaatgaaaa gttattaaga tggcttgatt tagctgaaga aaaaatttca tatcaaggac
    28021 taccatcacg tattgcttgg ttaggctatg gagaaagagc gaaaatgggc ttagctttaa
    28081 atcgtcttgt acgtgacggt gaaatttcag caccaattgt tattggacga gaccatttag
    28141 atgctggttc agttgctagt cctaaccgtg aaacagaaag tatgaaagat ggtagtgacg
    28201 cggttggtga ttgggctgta ttgaatgcac tcattaacac agctgcaggt ggttcatgga
    28261 tttcattcca tcacggtggc ggtgttggca tgggatattc acttcatgcg ggtatggttg
    28321 ttgtagcaga tggatcagag cgtgctgaaa gaagattgga acgtgtattg acgactgacc
    28381 caggtatggg tgttgcccga catgttgatg ctggctatga catcgctatt caaacagcta
    28441 aagaaaaagg tattcatatt ccaatgattg ataaagcagg tgataagtaa tgaatgattt
    28501 aataattaat catatagcag aattaatttt accgagatca acagataaac ctttgaaggg
    28561 taaggaatta gatgaattaa atgttgtgaa aaatggtaca gttgtcatta aagatggcaa
    28621 aattgtatat gctggaacac atacagatga ctacgatgcg actgagacga ttgatgctag
    28681 tgggaaggta gtgtccccag cattagtaga tgcacatacc catttaacat ttggtggatc
    28741 tcgagaacat gagatgtcat taaaacgtca aggtaagtct taccttgaaa tattagaaat
    28801 gggtggtggc atattatcta ccgttaatgc tactagagaa acatccgaag atgacttgtt
    28861 taaaaaagca gagcatgatt tgcttactat gattaaacac ggcgtgcttg cagttgaaag
    28921 taagagtggt tatggattag atagagaaaa tgaactgaag caattgaaag tgtctaatcg
    28981 tttagctgag aaatatgatt tagacatgaa acatactttc ctagggcctc atgctgtacc
    29041 taaagaggca agttcaaatg aggcattttt agaagaaatg attgcgttac ttccggaagt
    29101 aaaacaatat gcagactttg cggatatttt ctgtgaaaca ggtgtattta caatagaaca
    29161 atcgcaacat tatatgcaaa aagccaaaga agcaggtttt aaagtgaaaa tacatgcgga
    29221 tgaaattgat ccgttaggcg gactggaatt agcaattgat gaacaagcaa tatcagcaga
    29281 ccacttagta gcttctagtg ataaaggaaa agaaaagttg cgaaatagtg atactgtagc
    29341 tgtcctctta cctgcaacga cgttctattt aggtaaagaa gattatgcag atgcaagagg
    29401 catgcttgac aataacggtg cgattgcatt agcaactgat tataaccctg gtagtagtgt
    29461 cacaaacaac ttacaacttg ttatggcgat tgcagccttg aaattaaagc tatcacctaa
    29521 tgaagtttgg aatgctgtta cggtcaatgc tgctaaagca atagacatta acgcgggtac
    29581 aattaacaca ggtgataagg ctaatttagt aatttgggat gcaccaaatc atgaatatat
    29641 tccatatcat tttggtatca atcacgcaga aaaagttatt aaagacggta aggtcatcgt
    29701 agataacaca gtatctttta aagcataaaa taattagaaa gctctaagca ttggtttaaa
    29761 ctaatgttta gagctttttg tggtttgtaa aaacaagtat attcgagaac acgtttttgt
    29821 atagagtctc aattataaaa gttagaatag tttgacaaca gtgttacgat attattaaac
    29881 aactgtctat cattaacgaa agcaattaac aagagtacat tgttgtgaaa tcatcgttta
    29941 atagtcaaag atgatcatcg tagggtagac aacatatcat tgacttaagg aaggggaatg
    30001 accgatggga ttaaaagatc aagctgttac ctggagaagg tattttcatc aatttccaga
    30061 actatccgat aaagaattta agactacaca aaaaattaaa gatattttaa cagaacatca
    30121 tattagaata ttagacttac cacttgcgac tgggcttgtt gctgaagtgg gacaaggtct
    30181 gagttgtata gctgttcggg ctgacattga tgctttaccg attcaagagc ttgttgaaca
    30241 ggattttaaa tctgaaaatg aaggtgttat gcatgcgtgt ggtcatgata ttcacatggc
    30301 tagtattttg gctacagctg taaaattaaa agaaattgag ggcactctta ctgggcgtgt
    30361 taaattcatt tttcaatcag cagaagaact aggacatggc gcttttaaaa ttattgaaac
    30421 acatgcatta aaagatgtac aagcagtatt gggttttcat aatgacccat cgcgttcggt
    30481 aggtacattt gcaatcaaaa caggggcaat tacatcagca gtagatcgtt ttgagtttca
    30541 tattaaaggc gtgggtggtc atgctgcaaa gccagaacaa tgcaacgatc cagttattgt
    30601 gttggcgcaa ttgattaata gtattcaatc catagttagt agaaatctat ctgcgtttga
    30661 tgaagcggta gtaacaattg gacaaatatc atgtggtaat acttggaatg tcattgctga
    30721 tcatgcgtat gtacaaggca cagtgcgtag ttttgatcct gtggtacgaa agcttgtaga
    30781 aacacgatta caagatattg cggacggatt ggctcaagca tataatatga aaatcaattt
    30841 aaattatacg catttacctg gcgctgtgat gaatgatgag gcattgacgc ataaagcaat
    30901 tgcagtagcc caacatgttg gatataaggt ggaaatgatg gaacagccgc ttacgattgg
    30961 agaagatttt tcaggttata gtcaacattt tccaagtgta tttgcgttaa ttggttctca
    31021 tagtgaatat gatttacatc atcctcaata taaaccggat gaaaggattc tggaaaaagt
    31081 acctgagtat tttgttgaat ttgtaaagag actattgcat gaataattcg agcttgtttt
    31141 tataaagaag aaagtaattt ttagtgtaat catgattaag ttcctaggaa gattgggtaa
    31201 ataataacca ttaagcgaat aaggagatgt aaaaaaatgg cagctcaaga tcctagaaca
    31261 aaatttaaaa caacggatta tgaaaaacaa gaacaagagg taccgggttt acaatctgaa
    31321 atgacgccag cacccgattg tggcgaaacg tcttaccaag gtcatcaacg cttacaaggc
    31381 tataaaatat tagtcacagg tggtgactca gcaatagggc gtgcagcagc aattgcgtat
    31441 gctaaagaag gtgcagatgt agcgattaac tatcttccga gtgaagaaca agatgcacaa
    31501 gaagtacgcc aagtgattga agaaagtggt caaaaggcag tgttaattcc tggtgatata
    31561 agagatgaac agttcaacta tgaccttgtt gaacaagcat atcaacaatt aggtggctta
    31621 gataatgtta ctttagttgc tggacatcaa cagtatcatg atgatattca tggatttaca
    31681 actgaggcat ttacagaaac atttgaaacg aatgtctatc cgctattttg gacagttcaa
    31741 aaagcattgg aatatttaaa accgggtgcg tcaattacaa cgacatcatc tgtacaaggt
    31801 tataatccta gtcctattct tcatgattat gccgcttcaa aagcagcaat tatttcatta
    31861 actaaaagtt tttcagaaga attaggacca aaaggaatta gagtgaactg tgtagcgcct
    31921 ggtccattct ggtcaccatt acaaatttct ggtggacaac ctcaaagtaa aatcccaaca
    31981 tttggtcaaa aaacaccttt aggtcgtgca ggtcaacctg ttgagttatg tggaacttat
    32041 gtgctattag cgtcagaaga atcaagttat acaaccggac aagtgttcgg tgttagcggt
    32101 ggagtgcaaa tagattaggt ttgtatgaag cttttaattg aacaacataa tattaattta
    32161 gttacttcaa tccattaaat taaaatgaat tggtgaagtg tattttaaat agtcggtaaa
    32221 ggtgtgtatc tttatcggct tattttaata ctctgtcata aggagcgttt gctcagggtg
    32281 tggattataa gttttaagca gaataaactt tcttaatgag gatgtcagga caacaacgaa
    32341 aaagttaaaa actgttgcgg gattgggttt agggacggtt agtggaagaa agttaaattc
    32401 attaacaagt gaaatgccta aggctagata ttaaacgtca gaacggacaa agaataatag
    32461 gatatgtttt caattaaata gcgaagtatt tatatgttaa gatgatgtaa attacataga
    32521 aacgctaccc gtagaagtac tgatcataga aacgctgata aacgtaaatt tgcaaactcg
    32581 atattaaaca aaggggttgt tattatggca ttaatagtat ctagagctat attatttttg
    32641 attttagcat tagcttttgg aatagcgact gtttttcctg aacaaaaatt accattatat
    32701 atagtattca cattatttgt agcagttgcg atcgtttcga atgtatggtt tacacatcaa
    32761 aacgttaaaa agcatatcat taagtttaac aaatacgtcg catacttttt atcctttgtc
    32821 tcagggttac tttacattgg atttgtaatt tatacattaa ctatagggag ctatgatgtt
    32881 ttaccaatta ttatatttgc aataagtatc gtattggttc aaatattaga aaatatactg
    32941 ggaatcagta taaaaaatac gcgtctatca aacaaacagt tgaaagtcat ctccaagcaa
    33001 cgtgttcagc aatttaaagt catatcatta atattagtaa gtgtgtctat cgtgttcatt
    33061 tttattgcat taatgcttaa atggtggctt ttacttgtta ttagcattat tgttttgatt
    33121 attattacag tagcaatgat tgtttatgaa aaatattata cgactcctga ttaatttatg
    33181 attgacgtac taaggattat cttgtaacgt aaacgtaaat aagcatcact tatctagaga
    33241 ggtggaggga ctggccctat gaagcctcgg caacatctcg aatgtgccaa ttccagtaac
    33301 cgtaatggtt tgaagataag caggtaaagc acatgaaaac ctctttcttc atcgtttgtg
    33361 agaaagaggt atttttaatt ggaaagcagg taaaaaggat ggaagtacat aaaaagagca
    33421 atgcttgggc attattcccc ttgttattat ttgtggcgtt gtttttaggc gtaggtatta
    33481 tcacaggtga ttttacttca atgccattaa atgttgcaat tacgataacg gtaattgtgg
    33541 cgttattaat gaatcgaaaa gaatcatttg ctaaaaaagt tgaggtcttc acgaaaggtg
    33601 caggccattc aaacattgtt ttaatgatgt tgatttttat tttagcaggt gcattttcaa
    33661 atacagctga aaaaatgggt ggcgtaaagt cgacagttaa tttaggacta tcattaatcc
    33721 cagaaaattt aattattgta ggattgtttg tgatttgtat gtttgtttct atatctatgg
    33781 ggacgtctgt aggaactgtt gctgcaatag cacctgttgg ttacggattt gcacaagcga
    33841 cagatgtacc aactgcgcta gcaatggcta cagttgttgg aggtgcaatg ttcggcgata
    33901 atctatcaat gatttcagat acaacaattg ctgcggttag aacacaacat acgaaaatga
    33961 aagataaatt cagagtaaac tttaaaattg tattgccagg tgctatttta acgattatta
    34021 ttctgtattt cttaacgaat ggcatttctt taaatcatgc taaaaactat gattataatc
    34081 taattaaagt tgtaccgtat atcttagtat tggtattagc attattaggt gtaaatgtca
    34141 ttatcgtatt gattggcgga acattattag ccggaatcat tggtctcata gatggttcat
    34201 tcggttggat ggggctacta gatgcagtgt ccaaaggtat tataagcatg gaagatattg
    34261 ctatgattgc gttgctaatt ggtggtttag tcggtattat tcaacacaat ggtggtattg
    34321 aatggttgtt gcaatttgtt cgctctaaag tgaaatcaaa gcgtggcgca gaattaggaa
    34381 ttgctagttt ggtaagtgtc gcagatattg ccacggcaaa caacaccatc tctattatta
    34441 tgtcaggtcc tttagctaaa aatattgcag atgaatatga tgtagattca aggaaatcag
    34501 caagtattct tgatatattt ggaggctgct tccaaggttt attaccatat agcccgcaag
    34561 tcatttcagc ggctggtgta gctggtattt caccgttaat gttattccca tacagcattt
    34621 atcctatatt attaggtgta tgcggactta ttgcgatttt atttaatttt ccaaagctaa
    34681 aaaagaacac tagtcaagaa atgaaacatt aatgcttaac aatatatcga tgtagccaag
    34741 taaggtggct tgttatatta acagatcatg ttgtactcac cgtataacgc taatctttta
    34801 aaaatgatga tcatctatgt ttggaactga gcaaaaatac gttaaaataa aagtaagagc
    34861 gaaagtgggt ggacaacagt ggcaaaattt aatgtagaga atgaacatgt cgaagttgaa
    34921 attgaaaagc tttataaatt ttctccagag ttagtatacg aagcttggac aaagaaagat
    34981 ttgttgaaac aatggtttat gacatcagcc agaacgaata aagagattga agcggacgtt
    35041 aaagaaggcg gtaaatatcg tattgtagat caacaacgta atggcaaagt aaatgtgatt
    35101 gaaggcattt atgaatcttt agttatggat gaatacgtta agatgactat cggtatgcct
    35161 ggactaagtg aaacgcaaga tgtcattgag gtagaatttt ttgaacgtga aacaggtggc
    35221 acccaaatgc tattttatta tcgttcatta gttgaaaaag aaagacgatt tactaattta
    35281 gaatacaagc aaaagaaaaa agaatatcat gatgccatgg tgcatggttt cgaattgatg
    35341 tttgataaga tgtaccacgt tatcgaaaca tcaacacaac aataaaatga taaaaggaag
    35401 ttatgatagg ctcaaggcca ttaaatcata acttcctttt ttctaatttg aaatatcatt
    35461 atgtagtaaa tactcattat attttcttac aagtgtttca aagaatatat gataactgat
    35521 gaagctagaa tagtctgtac catcagttaa gtagaatgtg ttactggata catagacgtt
    35581 gtaatttgct ttttgagcaa gggtattatc tttcattgta gtaacagaaa taaagtattt
    35641 ttgtctaagt tgaagcaagt ttgttacttc taataattca tgtgtttcac ctgataatga
    35701 aataataaag aataaatcat taggttcact cttgttcaaa atcattttaa gttcatgttc
    35761 atcatgaagt acaatgacat gattatgtaa cgttaacaat atacgctgtg cctctgaagc
    35821 aacgtttaac tgtgcacgcc ctgtaccata caaatatatt gttgaagctg tatgcatttt
    35881 tttagtaaga agctggtaat caacgcgttc taaataactg aatgtactat cgatttcttg
    35941 tttgaaacta tcgatagagt tagaaggtaa ttggtgtgtc ttattacttt caaatttaag
    36001 gtacgattta aaatcactat aaccgtcaaa acctaatttg cgtgtaaatc tgtgaatggt
    36061 tgcattcgag gcatgtgtga attgcgctaa atcttgtatt ttcatatttt tacattcatc
    36121 tatatgtgta ttgataaagt gagcgatatg taaatcatta tcattaagtt tatcaaagtt
    36181 tcgattaatg tgttcatcta aaaacatatt caacctcctt gaaaataaat gtgtaataga
    36241 tcgttattac attaaaatct tgtatgtcat tatatatatt tgtgtattga tataggtttg
    36301 attatgcgtt taaaacgaat gtattacaaa tacaacgatg tcatatataa ggtcgtgaaa
    36361 aaattttcac tatatgaaaa gataatctga ttatacaaaa tactttcaaa atacgtcaat
    36421 gtcgttgtag gtgtgtaagc gtttactata tattatattt aaatagtaag acatctattt
    36481 ataaatgaat ctaattagat gtattgttag gaaagatgat atggagggaa tagaagatga
    36541 atgcgataaa gcgtttcggt agtgcaatga ttgtaccggt attaatgttc gctttctttg
    36601 ggatagtgct tggctttgcg acattattta aaaatcctac aatcatgggg agtttagcag
    36661 atcaacatac gttttggttt aaattttggt cagtaataga atctggtggc tgggttattt
    36721 ttacgcatat ggaagttgta tttgttgtag gtctaccact ttctttagct aaaaaagcac
    36781 caggtcatgc agcacttgca gcattgatgg gttacttaat gtttaataca tttataaatg
    36841 ccattttaac acaatggcca catacgtttg gcgctaattt agaaaaaggc gttgaaaatg
    36901 tacctggttt aaaatcaatt gcaggtattg ccacattgga tacgaatatt ctaggtggta
    36961 ttattatttc agctattatt acttggattc acaatagata ctatagtaaa cgattaccag
    37021 aaatggttgg tgtattccaa ggtttaacat ttgtagttac gatttcattt tttgtgatgt
    37081 taccattagc agcaattact tgtgttattt ggccaactgt tcagcacggt attggttcaa
    37141 tgcaacactt tatcattgct tcaggctata tcggtgtatg gttgtatcac ttcttagaac
    37201 gtgtattaat accaacagga ttacatcact ttatttatgc accaattgaa gttggaccag
    37261 tagtagtcaa tcacggcttg aaagctgaat ggttacaaca tttaaatgag tttgctaaga
    37321 gtactaagcc attaaaagag caattccctt atggctttat gcttcaagga aatgggaaag
    37381 tatttggatg tttaggtatt gctttagcaa tgtatgctac gacacctaaa gaaaatcgta
    37441 aaaaggtggc ggcactactt attcctgcca cattaacagc agtcgttgta ggtattactg
    37501 agccattaga gtttacattc ttattcatcg caccatactt atttgtttta cacgcagttt
    37561 tagcagcatc aatggatact ttaatgtacg catttggtgt tgtaggtaac atgggtggtg
    37621 gcttgttaga ctttatttca acaaactggt tgccattagg taaagaacat tggggtactt
    37681 atgttgcaca agttattatc ggtctgatat ttgtagcgat ttacttcttc ttattcagat
    37741 tcttaatttt gaaatttgat attccattac ctggacgtaa gaaaacagaa gaggaagtta
    37801 aattattttc taagcaagat tacaaaaata aaaaaggtga tagcgtagat tctaaacgtg
    37861 cttcatctgg taatgaatac gaagacaaag ctgcatatta cctcgacggt ttaggcggca
    37921 aagaaaatat caaagatgtt actaactgta caacaagatt acgcttaact gtttatgatg
    37981 aaagtaaagt tgcagatacg gaatatttca cgcatcaaca aatggcacac ggattagtaa
    38041 agagtggtaa gagtatccaa gttgtagtag gtatgacagt accacaagtt cgtgaagcat
    38101 ttgaacaaat ggttgaggat caatcgtccg aagataaata atacaaaaaa agacacatca
    38161 ctatacgcaa tcattgtaaa tgatcatcgt atagatgtgt cttttattat gatatacgag
    38221 ctttgctcat agatgacgat ctataattgg cagatagaat gtcgatattt ctagttgcaa
    38281 tcgtatgtaa caatttagca aatatttctg gcgtacatat aatatgtgca tgagacttat
    38341 tcaacgtata agtgatatca gtgtgatttt ggaacatatt cataatatcg atgactttgt
    38401 ctttaatgaa agcatcttgc aagccatcaa tattgatatc gaaacgcttt gcagattgat
    38461 ataaattatc agaatcaagt aattcattta ttttctttgg tgaaagtact tggaaaatac
    38521 tatactgatg aaaacttaaa aatgaaatga agtatggaag ttgttgcttt ggtaagctaa
    38581 tttctaatac atactgatta aactgtttgt ttattgagaa ttgcaacgca tcacaaaatt
    38641 gtacactttt gcataactta cttaattttt ttctagtagt acggtcattt aaaccggcga
    38701 tgttgatgat gtatgtttcg aatttatcaa ttaacatgtg aggacctccc gaggaataca
    38761 tggcattaaa tacacgttta atatttataa aggtgactta attttgttca agttgatttt
    38821 accacgcttt ttttctttat tcactaagac ttttgaatga agtttaaaat aattgtttat
    38881 cagtgataaa atatttgcaa taagaagaga atgactaaat aatcttaatt ttcagaaaag
    38941 taattgtaac cttactggtc ttatggtaat atttttcaat attatcgacg aggatgtgtt
    39001 aacaatgttt caaaaattga gtatatttgc cacgaagagt tttttggtat ggatgttagt
    39061 agcagctgtt attggattta ttttcccaca acatgttgcg acattaggta aatgggtacc
    39121 ttatttactt ggtattgtta tgttaggtat gggattaaca attacaccta atgatttcaa
    39181 aatggtcttt aaagcaccta gagcagtaat tattggtgtc tgtctacaat tcagtattat
    39241 gcccacatta gcatttataa ttgcaaagtc ttttcattta ccacctgata ttgctgttgg
    39301 cgtaatatta gtcggatgtt gtccgggtgg gacatcaagt aatgtaatga gttatttagc
    39361 caaagctaac gtagcacttt ctgtttctat tacgacggtc tctacgttgc tagcgccatt
    39421 cgttacacct gcgttaatat atctatttgc aaatgaatgg ttggaagtat ctttcgtgag
    39481 tatgttgtgg tcagttgttc aagttgtatt aattccaatt gctttaggta ttgttttgca
    39541 aattattaat cgtaaaattg ctgaaaaagc ttctacagct ttgccaatta tatcagttgt
    39601 tgctatttca ttaattttag caatagttgt aggtggcagt aagcaccaaa tcttaactac
    39661 aggattatta atatttttag tagttatttt acataacgta ttagggtata cgattggata
    39721 ttggttagct cgtcttttaa aattagatcg acaagatcaa aaagcagtca gtattgaagt
    39781 tggaatgcag aactctggtt tagctgtgtc attagcagca ttgcatttta atccaattgc
    39841 agcagtacca ggcgcagtgt ttagtttcat tcataatata acagggccta ttttagcaaa
    39901 gtattggtca aaaaagttat aattgcacta atagaatgaa gtggtcatcg gactatgtta
    39961 agctttgata aagagaaaaa atagaggagt aaatatatgt atagagcagt tatatttgat
    40021 ttcgatggaa caataataga tacggaacaa catttattta atgttattaa taaacattta
    40081 gagatgcata atgccgatcc tataagcatt gatttttatc gttcttctat tggaggagca
    40141 gctacagatt tgcatgacca tttaattaaa gcgattggtt cggaaaataa agataaactt
    40201 tatgaagaac atcatcttac tagtacaaca ttaccgatga ttgatacgat taaatcattg
    40261 atggcatttt taaagcaacg tcacattcct atggcaattg ccacaagtag tgtgaaagcg
    40321 gaaataatgc ccacctttaa agcattaggt ctagacgatt atatagaggt agttgttggt
    40381 agagaagatg ttgaacaagt taaacctgac cctgaattat atttatctgc agtacaacaa
    40441 ttaaattata tgccgacaca atgtttggct attgaagatt ctgtaaatgg tgcaacagcc
    40501 gcgattgcag ctggattaga tgttattgtt aatacgaata aaatgacaag cgcacaggac
    40561 ttttctaatg tagattatgt agcaaaagat attgattacg atcaaattgt agcgcgtttc
    40621 tttacgaaat aggaggcgta tcatgatggg ttacattata ttgttttttc tagctggtcc
    40681 agtaatttta ggcgttggaa atttggtgat tggtcctata tttaacaaac agacaccatt
    40741 tcgcgtgcaa gtaagatctt ttgttgttgg ttcaatgatt tacttaatac tcgcaacaat
    40801 tggctatttt ttactattac aaggtaaact ttaacgagaa aaccacctta cctcattaaa
    40861 tggacgacca tatgtatgtg aaatggtaga acgttcatgt ttatgtatga gatagggtgg
    40921 tttaaatagt tacatatatt ttaataataa cgtcacgatg ataagtacaa ttaagataat
    40981 atctatgcct accataattg tagctcttgt tgcattactt ccttgttctt ttgctgattt
    41041 catagcacgg tagtttggca caaagctaat aattagtaag attaatacaa ttacaccaat
    41101 taatgctgtt gtcatgatga acgacctcct ttattttttt caatcaattc ccaaataaac
    41161 gtagcaatca caccgatgat taacccagtg atgacaccga tttgtaccgt taaaaatatg
    41221 gcgccgaaaa tgataccaat aattaatgca atcggtaaag tcgtaccgag tttaatcttg
    41281 cgctctggag aattaataat agtaaatact gtaagacaaa tgagtatgaa agcaagtgtt
    41341 gcaataatag ttcttccaac taaatatagg atgtcaggtt tttccatacc gatataattt
    41401 atgatgaaaa atgctacagc aaagagtacc gatatttttg tagcacgtag cagtatttgt
    41461 tttaacattg atatactcct ttttaatatt attaaaatta tatcataatt accaagaata
    41521 gctgaagttg tatgtgactc aacggtactt gagcaacttt tttaattttt tagaaaaatc
    41581 acaaaataat tgtttgcaaa gttgcaaaag cctgctatag tagttctgta aacgattgca
    41641 tggtatgcaa atattaatgt accaaaatcg ataatttata gtataattac ggcaataagt
    41701 ttttttatgg atttatttag tatcaatcag agatggggta agaagttatg gagaacaatg
    41761 aactacaaag gggattgagt gcccgtcaaa ttcaaatgat tgcacttggt ggtacgattg
    41821 gcgtggggct tttcatgggt gcgacaagta caattaaatg gacaggccca tcagttatcc
    41881 ttgcatattt aattgcgggt atctttttat ttttaatcat gagagcaatg ggggaaatga
    41941 tttatttaaa ccctacaaca ggatcatttg caacatttgc aagtgattat atacatcctg
    42001 cagcaggtta tatgacagca tggagtaata tattccaatg gattgtagtt ggtatgagtg
    42061 aggtcatcgc agtaggagaa tatatgaagt tttggttccc ggaattgcca acttggattc
    42121 ctggtgttat tgctatttta ttattaatgg cagcgaattt attctcggta aaagcgtttg
    42181 gagaatttga attttggttt gctttaatta aagttgtaac aattatttta atgattattg
    42241 ctggttttgg tcttattttc tttggttttg gaaatggtgg ccatgcggta ggtatttcta
    42301 atctatggac aaatggcgga tttatgccaa atggtattgt tggtttcttc tttgcattat
    42361 caattgtaat tggttcatac caaggtgtgg aactgatagg tattacggca ggtgaaacta
    42421 aagatcctca gaaaaatatc gttaaagcag tgaatggtgt tatctggaga attttaattt
    42481 tctatttagg cgctattttt gttattgttt cagtgtatcc ttggaatcaa ttaggagaca
    42541 ttggaagtcc gtttgttgca acatttgcta aaatcggtat tacatttgca gctggattaa
    42601 ttaactttgt tgtattaact gcagcaatgt caggatgtaa ctcaggtatt ttcagtgcga
    42661 gtcgtatgat ttatacactt gctcataaag gtgaaatgcc gaaaatattt actaaaatca
    42721 tgagaaatgg tgtaccattg tacactgttg tagcagtatc tcttggtatt ttaattggtg
    42781 ctttattaaa cgtaatttta cctttatata tcgatggagc gaagagtatt ttcgtatacg
    42841 tttatagtgc gtctatttta ccaggtatga ttccttggtt tatgatttta ttcagtcatt
    42901 tacgttttag aaaattacac cctgaagaat tggaaggtca cccattcaaa atgcctggtg
    42961 gcgcagtaac taactattta acaatcttat tcttaatatt agtattggtt ggtatggtat
    43021 ttaatgttga aactagaata tcagtgctta ttggtgtcat cttcttaacg attgtaacga
    43081 tttattattt tattagatat aataaaaata atgtaaaagc aaaatagtat gattataatg
    43141 tgcgcttatc ttatgacaaa cttggcccta gcttattaaa gttaaatagc caaagaatgt
    43201 tatgagtagg cgcatttttt tataaatata gcttaaatcg aagttgtagt gtgaaaaact
    43261 tataagtggc aatattatcg ttagttttta ctgtgggctt taaaataaaa aatagcatta
    43321 ttaaaataac aaacgcaatt gatatatgga atgaatccgt taatcaactg cgtttgtttt
    43381 taatataaga aattgaatgg ccttatcaaa tcattggtca tatgcgtatg ttatacaaga
    43441 taaaagacat gtaagctttg aaaatgatgc ataaattatt atatgacatg attaaagaat
    43501 ttatataaga ctgttaatgt ttaaagttaa tatttgataa atgtttacga tagttatcta
    43561 aagccatttt agattgtgtt atgaaatcta atgatgcgtg ataatttaat gcaacataac
    43621 gataatataa aatatcaata gtgaacattt gagcaaataa tgaagttgtt gctcccatat
    43681 gcatctcatt ttcatcagtt ttcccataag ttaaaacaat atttgatgcc tgtgctacgg
    43741 gattatccct tgtactagta attgtaatta taggtatatg gtagtcatca ataactttaa
    43801 ccattgattg catttcactt tgcgtaccat tgttagtaat aagaataaca ctgtcgttcg
    43861 aattgtgagt tgctaataat gtagcaaaaa tatgtgtttc ttgaacaagt tgaatattaa
    43921 gacctattct tgataacttt tggtataagt cggtagcaac tacaaaagat gcgccaaaac
    43981 catatataaa aatcgtctca gaacgtttta aacaatgaca tatttgatca atagttttat
    44041 catttaattc gttatttgca tgattaagtg cgcgtgtagt acgtgtatgg agtttagttc
    44101 ttaaagattc tgtgctttcg ttattcatta attcaacatt gtaaattgat gatgctttag
    44161 gaacatattt agatatattt attttcaagt cgtgaaaacc gccatcagta attttcttgc
    44221 tgaatcgaat gactgatgat gtactaatat ctaataagct tgctaagtct tgagacctca
    44281 ttttaatcac cttttgaggt gaatttagta taaatttagc tatctttttt tcatttttag
    44341 tcatatatgg atattgacta tctatttctg ttagtacgtt tgacatatta atcactccac
    44401 tttaacgcaa tatattttat tagcgttggc tattgttgtt taacgcgatg gtatttacaa
    44461 cttgtaacgt aagagaatga aaaataagct gaatatgttg tttgtttaat gatattgaat
    44521 tgtaaaactg taaatttttt taagatgtat agataaaaaa tagcatactc ccatgtgcaa
    44581 ataatatatc aatatattga tatattaccg taagtcatac tgtatattcc caatttatac
    44641 attatgtact catctattta aatcttagta aatgtagtta tataagacta aatctaccat
    44701 cttaatcatt atgattataa ctcgattaat gcatctttac aatttgaaga aactttttaa
    44761 ttgtataatt tatgtaataa agtgaatttt aataaataac ttgtttaaat gacattttat
    44821 gaaaaatagg ttaatgggca aatttaattg caaataatgc gttagatagt tctgtatgat
    44881 aaaaattaag atattgaatt ttgttaccat agtcgacttg tcggagggga cgtttattaa
    44941 gtagtgttat aatttcataa taggaaactt gattttcgtt tattatatat gatgtttcaa
    45001 agcctttgaa cttattcaat tcattagtaa ctatttggtt aaatttataa atatgcgttt
    45061 gaatgttcca atctgtcatt aaagtattat atttaatcgt atcaatgatt tgaatcaaat
    45121 tagtaatttg ttgtctcata tacttagttt cgtttatcat gtagaagtac ctccaaaaat
    45181 caattattaa acaggaaagg atataacagt ggaaaatgaa aaaaagaaat acacgtttaa
    45241 agatatagca tggagagatt tgcttataat tccaattata ggtgttatat tttatgcact
    45301 cacaattgca gcttacatag gaccaatttt gtttggttat tatgaaatcg atagtaatct
    45361 gatatttgta gcgactatca cacaagcttt agcatatacg attgggattg gcgcatttta
    45421 tttatttcat ttaaaagtga tgccatcaag gcttcgagct ggttttgcgt atatcaaaaa
    45481 atattggctt agattgtcaa taacgtatat tattgctctc atcttaattt atacatatga
    45541 atatatgaca cagtttctac ctaaacattt gcaatatagt gaaacagcca atgaacttga
    45601 gctaaataaa atgtttgaag tgcctgcatt tttacctgta gcattcttat taattgtgat
    45661 tgtaggtcca atagttgaag aaatcgtatt tagacatatt ttaattggag aattaggtaa
    45721 gaagtttaat tttatagcta tgagtattgt ttcggtgttc ttatttgcat ttattcatgt
    45781 gactgatgca aagtcgccgt ttgagtttgg tccatatctc atactatcca tcatattagt
    45841 tttcacttat ttaaaatctg gaagaaacct aggttcaaca attgcattac atatagcaaa
    45901 taattttgtt tctttcgtta tatcagtaat aagtatttat gcgtagataa agaggtcggg
    45961 acattaagtt tttaggcaat gaaa
//
LOCUS       DALAGF010000008        45083 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.008, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000008 DALAGF010000000
VERSION     DALAGF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 45083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45083
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.008"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            21..743
                     /locus_tag="O2692_000427"
     CDS             21..743
                     /locus_tag="O2692_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCZ4298075.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            complement(929..1204)
                     /locus_tag="O2692_000428"
     CDS             complement(929..1204)
                     /locus_tag="O2692_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298076.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(1359..2423)
                     /locus_tag="O2692_000429"
     CDS             complement(1359..2423)
                     /locus_tag="O2692_000429"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCZ4298077.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTAFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            2641..3507
                     /locus_tag="O2692_000430"
     CDS             2641..3507
                     /locus_tag="O2692_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCZ4298078.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRLDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDKILQDTTS"
     gene            3849..4127
                     /locus_tag="O2692_000431"
     CDS             3849..4127
                     /locus_tag="O2692_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HCZ4298079.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLMSKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            4142..4510
                     /locus_tag="O2692_000432"
     CDS             4142..4510
                     /locus_tag="O2692_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298080.1"
                     /translation="MSNKLDEINKIITAKHEQMDDLYDEKREVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKVEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            4498..4794
                     /locus_tag="O2692_000433"
     CDS             4498..4794
                     /locus_tag="O2692_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HCZ4298081.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTQQ"
     gene            5128..6234
                     /locus_tag="O2692_000434"
     CDS             5128..6234
                     /locus_tag="O2692_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HCZ4298082.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTEIYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVLTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC"
     gene            6228..7568
                     /locus_tag="O2692_000435"
     CDS             6228..7568
                     /locus_tag="O2692_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HCZ4298083.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQVLPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            7666..8556
                     /locus_tag="O2692_000436"
     CDS             7666..8556
                     /locus_tag="O2692_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCZ4298084.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQ
                     SPVKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(8703..9533)
                     /locus_tag="O2692_000437"
     CDS             complement(8703..9533)
                     /locus_tag="O2692_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4298085.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWLEFEIRQYTHSNITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(9553..10563)
                     /locus_tag="O2692_000438"
     CDS             complement(9553..10563)
                     /locus_tag="O2692_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCZ4298086.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSP
                     NN"
     gene            complement(10661..11365)
                     /locus_tag="O2692_000439"
     CDS             complement(10661..11365)
                     /locus_tag="O2692_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCZ4298087.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            complement(11358..11915)
                     /locus_tag="O2692_000440"
     CDS             complement(11358..11915)
                     /locus_tag="O2692_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCZ4298088.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVGRND"
     gene            12046..12345
                     /locus_tag="O2692_000441"
     CDS             12046..12345
                     /locus_tag="O2692_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HCZ4298089.1"
                     /translation="MSLNKEQRRITAEELQAHFEASTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            12365..13186
                     /locus_tag="O2692_000442"
     CDS             12365..13186
                     /locus_tag="O2692_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HCZ4298090.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            13203..13580
                     /locus_tag="O2692_000443"
     CDS             13203..13580
                     /locus_tag="O2692_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4298091.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(13730..13960)
                     /locus_tag="O2692_000444"
     CDS             complement(13730..13960)
                     /locus_tag="O2692_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCZ4298092.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(14180..14680)
                     /locus_tag="O2692_000445"
     CDS             complement(14180..14680)
                     /locus_tag="O2692_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298093.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICACLLANVRHSSIVCLNNIC
                     CIVDMRCLNTKIGGIDVSETDEPQGFERTHNILNINQSSLGVVTYITNKLKSTLKQHI
                     IIARGKKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            complement(14878..15447)
                     /locus_tag="O2692_000446"
     CDS             complement(14878..15447)
                     /locus_tag="O2692_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCZ4298094.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            15602..15730
                     /locus_tag="O2692_000447"
     CDS             15602..15730
                     /locus_tag="O2692_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298095.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSENSEFCDILFFIVLSYR"
     gene            15857..16912
                     /locus_tag="O2692_000448"
     CDS             15857..16912
                     /locus_tag="O2692_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCZ4298096.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            17520..18221
                     /gene="isaA"
                     /locus_tag="O2692_000449"
     CDS             17520..18221
                     /gene="isaA"
                     /locus_tag="O2692_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HCZ4298097.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            18497..18986
                     /locus_tag="O2692_000450"
                     /pseudo
     CDS             18497..18986
                     /locus_tag="O2692_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            19497..21308
                     /locus_tag="O2692_000451"
     CDS             19497..21308
                     /locus_tag="O2692_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCZ4298098.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
     gene            21669..22436
                     /locus_tag="O2692_000452"
     CDS             21669..22436
                     /locus_tag="O2692_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ4298099.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            22611..23099
                     /gene="crtO"
                     /locus_tag="O2692_000453"
     CDS             22611..23099
                     /gene="crtO"
                     /locus_tag="O2692_000453"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HCZ4298100.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            23092..24585
                     /locus_tag="O2692_000454"
     CDS             23092..24585
                     /locus_tag="O2692_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ4298101.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            24591..25718
                     /gene="crtQ"
                     /locus_tag="O2692_000455"
     CDS             24591..25718
                     /gene="crtQ"
                     /locus_tag="O2692_000455"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HCZ4298102.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            25755..26618
                     /locus_tag="O2692_000456"
     CDS             25755..26618
                     /locus_tag="O2692_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HCZ4298103.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            26630..28138
                     /locus_tag="O2692_000457"
     CDS             26630..28138
                     /locus_tag="O2692_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ4298104.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIERGV"
     gene            28565..29719
                     /locus_tag="O2692_000458"
     CDS             28565..29719
                     /locus_tag="O2692_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCZ4298105.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            29742..30740
                     /locus_tag="O2692_000459"
     CDS             29742..30740
                     /locus_tag="O2692_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCZ4298106.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNFIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(30830..31036)
                     /gene="copZ"
                     /locus_tag="O2692_000460"
     CDS             complement(30830..31036)
                     /gene="copZ"
                     /locus_tag="O2692_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCZ4298107.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
     gene            complement(31189..33597)
                     /locus_tag="O2692_000461"
     CDS             complement(31189..33597)
                     /locus_tag="O2692_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCZ4298108.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLILTETTTFKAVPGHGIEATIDH
                     HYILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
     gene            complement(33837..34028)
                     /gene="cwrA"
                     /locus_tag="O2692_000462"
     CDS             complement(33837..34028)
                     /gene="cwrA"
                     /locus_tag="O2692_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HCZ4298109.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            34267..34866
                     /locus_tag="O2692_000463"
     CDS             34267..34866
                     /locus_tag="O2692_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCZ4298110.1"
                     /translation="MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            35056..36600
                     /gene="pruA"
                     /locus_tag="O2692_000464"
     CDS             35056..36600
                     /gene="pruA"
                     /locus_tag="O2692_000464"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HCZ4298111.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            36712..36879
                     /locus_tag="O2692_000465"
     CDS             36712..36879
                     /locus_tag="O2692_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298112.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFIFFFIVYVLCISCF
                     NSDKHDKNKKR"
     gene            complement(36956..37504)
                     /gene="farR"
                     /locus_tag="O2692_000466"
     CDS             complement(36956..37504)
                     /gene="farR"
                     /locus_tag="O2692_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HCZ4298113.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDE
                     KIDWPGEDIDNIFHRLINIKIK"
     gene            37649..40138
                     /gene="farE"
                     /locus_tag="O2692_000467"
     CDS             37649..40138
                     /gene="farE"
                     /locus_tag="O2692_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HCZ4298114.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVVFAVLSALTLLPALISIFHKRI
                     KVNKLQSKFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDAHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQL
                     NSKKQATNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            40354..40581
                     /locus_tag="O2692_000468"
     CDS             40354..40581
                     /locus_tag="O2692_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCZ4298115.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            40593..42587
                     /gene="feoB"
                     /locus_tag="O2692_000469"
     CDS             40593..42587
                     /gene="feoB"
                     /locus_tag="O2692_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCZ4298116.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            42600..42770
                     /locus_tag="O2692_000470"
     CDS             42600..42770
                     /locus_tag="O2692_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCZ4298117.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     TEQHTANHFPGK"
     gene            complement(42839..44944)
                     /gene="clpL"
                     /locus_tag="O2692_000471"
     CDS             complement(42839..44944)
                     /gene="clpL"
                     /locus_tag="O2692_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HCZ4298118.1"
                     /translation="MNNGFFNSDFDSIFRRMMKDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGGNHSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
BASE COUNT        15103 a         6909 c         8392 g        14679 t
ORIGIN      
        1 taaatgtggg aggttaaatc atgattaatg ctgaacctat tatcagtaaa atgaaaaatc
       61 aaaaaattaa ttatgacaag gtgttgaaga aattaatcgg gcagtgggaa agagaagcaa
      121 tcagacctaa aatcttattg catagttgtt gtgcaccttg tagtacatat acattagagt
      181 ttttaacaca atatgcagac attgcaattt atttcgcgaa ttcaaatatt catccgaaaa
      241 atgagtactt acgacgtgct aaagtgcaag aacaatttgt ggaagatttt aatcgcaaga
      301 ctggtgcgaa tgtgaagtat attgaagcac cttatgaacc gcataagttt gtgaaaatgg
      361 tgaaggataa agaattagct gatgaaaaag aaggcggctt acgttgtacc gcttgttttg
      421 aaatgcgttt ggatattgta gcgaaagcag ctgtagaaca tggctatgat tattttggca
      481 gtgcaatcac attatcacct aaaaagaacg cacaattaat caatgaactt ggtatggatg
      541 tccaaaaaat atacgatgtg aactatttgc caagtgattt taagaaaagt aaaggttatg
      601 agcgttccat agaaatgtgt aatgactata atatctttag gcagtgttat tgtggatgtg
      661 tctttgcagc gatgcaacaa ggtattgatt ttaaaacagt taataaagaa gcgaaagcat
      721 ttttagaaca atatccagat taatcattgg aaatgagata aaaggcaaga acgtatacat
      781 gccatatccc attaaaaatt atcaaataga aacccgagat tttagtgaaa tgaatcatac
      841 taaaatctcg ggttatttat ataatcgtaa atattgtaga tataatgaat aactggatcc
      901 tgagacataa aacaatgtct cactctaatt acatcatttt atgaacaaat cttataattg
      961 ctaaaatgac aatgacaagt gttgaaataa gtgaaaacac tttaaacttt ttatagtcat
     1021 ttttcgtttc attttctcct gttttcatca tttcacgtag ctcttgctcg atttctaaat
     1081 caagatctgt tttacgataa ccattcaaac cttgacgttc atcttcttca ggttgatttt
     1141 gttgctgtgt gtctttgttg tcagaagtcg ctactgtttt tttattatct gtttctttag
     1201 tcataacaaa cgcctccgtt ataaaacgct atatttaatg atatgtgata tttaagccat
     1261 tattttgaaa aatggtgata aatatatctt cttgagttat atcataattt taataagacg
     1321 attcagcaag ttttaaagta ttatttgact atgttggatt aggcatctag tcctataata
     1381 tcactgacat tgtcaaaatg atgatctttt aagtaacgtg cgatgccttt gttcattttc
     1441 ttagttaaac ctgggccttc aataacaagt gatgaataaa tttgaataag tgacgcaccg
     1501 tgacgcatca ttttgattgc atcttcagta ctgaatacgc cgcctgtacc tataattaaa
     1561 aattcaccat ttgtttgctg ataagcatac ttaatcaatt ttaaattacg ttcaaataat
     1621 ggacgaccac tcaaaccgcc ttcttcgact ttattagcag aagttaaacc atctcgttgt
     1681 cgcgttgtgt ttgctaagat gataccgtca aatgtctcag taatcgctgg taatagtgct
     1741 tttaagccat cgaaatccat atcagacgtt agttttaaat aaattggcac tgttacatca
     1801 tgttgttttt taaatgctgt taaagcttgg cataacattg aaaattcatc tttatcatgg
     1861 aagttttgaa gattttcagt atttggagaa ctgatgttga ctgtgaaaaa tgaaacgtcg
     1921 tgtttaaacg tatcaataac ctttatataa tcttgataac gcgcttcata aggtgtcatt
     1981 ttattcacac caacattgat accaacaggt acttgataag catttttacg caaatgactt
     2041 agtgctttgt tcataccaat attattgaag cccattcgat ttatcaaggc gtcatcttct
     2101 aataatctaa acatgcgtgg ttgagggtta cccggttgag gtttaggtgt gataccacct
     2161 aattctaaag caccgaatcc aaggtgttcc aatgcttttg gtacttcgca agatttgtcg
     2221 aaaccagctg ctaagccaat tggattgtcg tacgtattac cttgtatcgt ttgtgataac
     2281 gttggattct tataagtaaa tagtttatcg acgactggga ataaaaccgg aaacttttgt
     2341 aacgttttta atgcatcgat agttagtccg tgtgcttttt cgggttcgat tttgaataag
     2401 aaaggtttaa ttaatttgta catgagtatg ctcctatttc attatatttg aggcttacta
     2461 tcctcaactt aatatatgtg aaatatattc ttttaataga ctagcatttc catacataat
     2521 ttcctagtta aaactaaaaa gttttgaaaa ttgacgcaag tttgaataac gtttttaaga
     2581 ttaaatcatc ctaattaggc aatattatag tataaagtaa gtagattgga aggtgtttgt
     2641 atgaatgaac aatggttaga gcatttacct ttaaaagata ttaaagagat ttcaccagtg
     2701 agtggtggtg atgtaaacga agcatatcga gtcgaaacag atacggatac atttttctta
     2761 cttgtccaac gtggacgtaa agaatcattt tatgctgcag aaattgcagg tttaaatgaa
     2821 tttgaacgtg caggtatcac ggcacctaga gtaattgcaa gtggcgaggt taacggtgat
     2881 gcgtatttag tgatgacgta tttagaagaa ggggcttcag ggagtcaacg ccaattaggg
     2941 caactcgtag ctcaattaca cagtcagcaa caagaagaag gcaaatttgg cttctcatta
     3001 ccttatgaag gtggcgatat ttcttttgat aatcattggc aagacgattg gtgtaccatt
     3061 tttgtcgaca aacgcttgga ccatttgaaa gatgagttgc taaatagagg gctatgggat
     3121 gccaacgata tcaaagtata tgacaaagtg cgacgtcaaa ttgtggcgga attagaaaag
     3181 catcaaagta aaccgtcttt attacatggt gacctatggg gtggtaatta tatgttctta
     3241 caagatggtc gtccggcgtt atttgatcca gcgccattat atggtgacag agaattcgat
     3301 atcggtatta caacggtatt tggtggtttt acgagcgaat tttatgatgc gtataataaa
     3361 cattatccac tcgcaaaagg tgcatcctat agacttgaat tttatcgttt atatttattg
     3421 atggtccatt tattgaaatt tggtgagatg taccgtgata gtgttgcgca ttctatggat
     3481 aagattttac aagatacaac aagttagtta agacgttaga ttgagataaa tagataatat
     3541 gcacagatat ttttacaatg agaagcgata cagctgcctc aataaaaata tttgtgcgtt
     3601 tttattgttg gaaaataaaa ttttaatcgc tattgttaat ttctgtaatg taaaacaagg
     3661 ttgagttaca ataaaagtga ttttataact ttttgttcaa taaaattcta ggaatgatac
     3721 atatttattg atacaataat tttgaatata atcataaaac aatatttaag tataattgaa
     3781 tgtttgaata tcatatattg atacagtttc taataatttt aaaataattt aaatggagag
     3841 aggtgtaaat gatgagtaca gttcaaagtg atatttttaa gaccaatagt gcatcatcat
     3901 ctattaaaag cgctgttgaa acatgtaata atgtgtcgaa accggataaa gatgaaagta
     3961 caacagtaag tggaaataat aatgctcata gtgtgataga tgatttgatg agtaagaatc
     4021 aatctgttgc tgaagcaata cgaactgcga gcgataatat acaaaaagtt ggtgaggctt
     4081 ttgaccaaac tgacgtaatg attggtaatg aaattggtaa aaattaaaac gtggtgaaat
     4141 gatgtcgaat aaactggatg aaatcaataa aataatcaca gcgaaacatg agcaaatgga
     4201 tgacttatat gatgaaaagc gagaggttaa agcattgata gatgaaagtg atgcgcttaa
     4261 tcattcgata gatcaattat atcaacattt aggtgagcgt tattatagta gcaatatggc
     4321 tagtcgtatg gaacagttcc gcgatgaatt tcattttgcg aaacgacgtt caacggaagc
     4381 gttatacgag cagcaacagc aaattcaaca tggcattcgt aaagtggaag aagagatgat
     4441 tgacttggaa atgcgaagga atgttgaaat tgagacggtg acaaaggagg aaaataaatg
     4501 gaaacaatag gaagcattat ttatttaaaa gaaggttcgc aaaagttaat gattattaat
     4561 agaggaccaa ttgtagaaat tgaaaatcaa aagtatatgt ttgactattc tgcatgtaaa
     4621 tatccgattg gtgttgtaga agatgaaatt tattatttta acgaggaaaa tatagattca
     4681 gttattttta aaggttattc tgatcaagat gaggttagat ttcaagagtt gtttgaaaat
     4741 atgaaacaaa atttggatag tgaaatacaa cgtggagaag ttacacaaca ataaagaaat
     4801 actttttctt tattggggtg ggacgacgaa ataaattttg taaaaatatg atttctgtct
     4861 caccgccaaa atgttatctc caagtactga ggctggatga taccatgaaa tgtcattcat
     4921 aatcatttga actaaacgta gcagccttaa attttaaaaa aagacacata ccaacttccg
     4981 aaatgtagat gaattctcta caataacgga agtttttctt ttaatattga aatttctcaa
     5041 ggataggtct atactttata aatcgtaatt attacgattt ataatcaaaa acaataactt
     5101 gaaatagatc attgagggag tgttaatatg caacatcata aagtggctat tatcggtgcc
     5161 ggtgctgcag gtataggtat ggccattacc ttaaaagatt tcggtataac agatgtcatt
     5221 attttagaaa aaggaacagt aggacattca tttaaacatt ggccgaaatc gacccgtacg
     5281 atcacgccat catttacgtc taatggattt ggcatgcctg atatgaatgc aatttccatg
     5341 gatacttcac cagcatttac atttaatgaa gaacatattt ccggagaaac atatgctgaa
     5401 tatttacaag tggttgccaa ccattacgag ctgaatatct ttgaaaatac agttgtcaca
     5461 aatatatctg tagatgatgc atattatacg attgcaacga caacagagat atatcacgcg
     5521 gattatatct ttgtcgcaac aggtgattat aatttcccta aaaagccatt taaatatggt
     5581 attcattata gtgaaattga agactttgat aactttaata aggggcaata tgtggttatc
     5641 ggaggtaatg aaagtggctt tgatgctgca tatcaacttg caaaaaatgg ctctgacatc
     5701 gcactttata ctagcacaac cggtttaaat gatccggatg ctgatcctag tgttagattg
     5761 tcaccttata cacgtcagcg actaggtaat gtcattaagc aaggtgctcg catcgaaatg
     5821 aatgtacatt atacagttaa agatattgat tttaacaatg gacagtatca tatcagtttt
     5881 gatagcggac aaagtgtgct tacacctcat gaaccaatac tagcaactgg ctttgatgca
     5941 acaaaaaatc caatcgttca acaattattt gtgacaacaa atcaagatat taaattaaca
     6001 acacatgatg aatcgacacg ttatccgaat atttttatga ttggtgcaac agttgaaaat
     6061 gataatgcca aattatgcta tatctataaa tttagagcgc gatttgcagt acttgcacat
     6121 cttttaacac agcgggaagg cttaccagct aaacaagatg tcattgaaaa ttatcaaaaa
     6181 aatcaaatgt atttagatga ttattcatgt tgtgaagtgt catgcacatg ttagaagtga
     6241 aatatgatat gagaactggg cattatacgc ccatacctaa tgaacctcat tatttggtta
     6301 ttagtcatgc ggataaactt accgcaacag aaaaagcgaa attaagatta ttaatcataa
     6361 aacagaaatt agatatttca ttggcagaaa gtgtagtttc ttcgcctata gcgagtgaac
     6421 atgtgataga acaattgaca ctatttcaac atgagcgacg acatttaaga cctaaaataa
     6481 gtgcgacatt tttagcctgg ttgttgatat ttttaatgtt tgcattgcca atcggtatcg
     6541 cttatcaatt ttcagattgg tttcaaaatc agtatgtgtc agcatggata gaatatttaa
     6601 ctcaaacaac attgctcaat cacgatatat tacagcatat attatttggt gattatggtg
     6661 tgctatcact tggaacatat tcgctcgtat gggcattgcc ggttgtaata ttgattagtt
     6721 tatcaactgc tataattgat caaacaggac tcaaatcatg gatgatatgg gcaattgaac
     6781 cgtcaatgtt atggatagga ttacaaggta atgatatcgt gccactatta gaagggtttg
     6841 gatgtaatgc agcagctatt tcacaagcag cacaccaatg ccatacctgc acgaagacac
     6901 agtgtatgag tttaataagc tttggtagtt cttgtagtta tcaaataggt gcgacattat
     6961 ctatttttag tgtagctgga aagtcatggc tatttatgcc gtacttaata ttagtacttt
     7021 taggtggcat cttacataat aggatatggt tgaaaaagaa tgatcaacaa cttagcgttc
     7081 cgctacctta tgataggcaa ttacatatgc caaatatacg tcaaatgttg ctacaaatgt
     7141 ggcaaaatat acaaatgttt atcgttcaag tgctacctat ttttatcaca atctgtctta
     7201 ttgttagtat tttatcacta acgccaattt tgaatgtttt atcacaaata tttacaccta
     7261 tattatcgtt attaggcatc tcgtcagaat tgtcaccagg gattttattt tcaatgattc
     7321 gaaaagacgg catgctcttg tttaatttgc atcagggcgc cttattacaa ggaatgacag
     7381 caacacagtt actactactt gtgtttttta gttcaacatt tacagcgtgc tcggtcacaa
     7441 tgacgatgct tttgaaacat ttaggtggtc agtcagcact aaaattaatt ggaaagcaaa
     7501 tggtgacatc attgtcttta gttattggtg taggcatcat tgttaaaata gtaatgctga
     7561 ttatttaaaa aaaatgaact ataactgaat atagagtcat gtcagtcaat aggagatcta
     7621 tcttggaata tgctattcat atgaagtata agaggagagt cgcagatgaa aatagttatt
     7681 ataggtgggt ttttaggtgg cggtaaaacg actgtcttaa atcatttgct cgctgaatca
     7741 ttaaaggaat cgctgaaacc agcagtcatc atgaatgaat ttgggaaaat gagtgttgat
     7801 ggtgccttag tatctgaaga cataccttta agtgaactga cagaggggtg tatctgttgt
     7861 gcaatgaaag cagatgtatc agaacagtta catcaattat atttaaaaga gcaaccagac
     7921 attgtattta ttgaatgtag tgggattgca gaaccggtct ctgtcttaga tgcttgttta
     7981 acgcctattt tagctccgtt tacaacaatt acacatatga ttggtgtaat agacgcaagc
     8041 atgtataaac acattaaatc attccctaaa gacatccaag gcttatttta tgagcaatta
     8101 gcatattgtt ctgtcttatt tgttaataaa atagattcag cagatgttga aacaacgagc
     8161 aaactattga aagatttaga agttattaac ccagaggccg atatacaagt cggtatgcat
     8221 ggcagcgtca ctttgccaat atcagttaga caaatgacag caacttctga caataaacat
     8281 aagtctttac atcaaatgat taatcatcaa tttgtgcaat caccagtcaa atgtactaaa
     8341 gcagagttta taaaacgttt agcatgcctt ccgtctcata tttataggtt gaaagggttt
     8401 atgacatttg aagacaccgc acatacgtat ctcattcaat ttacacaagg acaatatgaa
     8461 ttaacacctg tagcattttc aaaaaaagtg ccagaatatt tggtgcttat cggaaagggt
     8521 atttcaaaag aagactatca atgtttggaa cagtagtgtt ttcagtggaa gagaatggtt
     8581 aacatgcctt catgtataat aacgagttga tttgaacgtt taagcgtaaa taaaaataag
     8641 cttggtcagc catcaaatat aatttgaaaa ctgtccaagc tgttttatta gagaacaatc
     8701 aattaacccc acatatttaa taatacatca gcaaagcctt caggtttttg aatataacct
     8761 aagtgaccgc ctggaatatc tacaataggt atgccagttt ctttatttat ataaaagtta
     8821 acatcttgtg ggaaggagcc tctagaatct gtcccattta gtagggtgat tttatcgctg
     8881 tattttgtga aatcatccaa agtaatattt gaatgcgtat attgtctaat ttcaaattct
     8941 aaccagaaca tcgtacgttt gtactgttct atacgtcctt cttcagtatc agcaggttga
     9001 gacatcattt ttgcatcaat tggtgcgata tttaatgttt cgccaaatgt tttcatgcct
     9061 ttttctaagc cttctgttaa aatttgatgc acaatgtcat catttttatc tttccaataa
     9121 gtactgtctg gtaaaaatgt attaattggt ggttcgtgaa atgcaatctt tttaacgact
     9181 tcagggtaat cttttaacac atgcatcgca acgattgaac ctgaacttga acctaatata
     9241 tagacaggtt catcacttaa tgactttgca agttcggcaa tgtcctgtgc gtcgcgtttg
     9301 acacgataat cactgtcagg gtttgaagcg gaatcaggga gtggttcagt taactcgctt
     9361 tctccataat cacgacgatc aacggctaca acagtaaaat ggtcttttaa ctgttctgca
     9421 agaggcagaa aaatgtctcc ggtaccgttt gcaccaggaa taaagatgag cacgggtcct
     9481 tgtccgactt ggtggtatcg taatttagcg ccttgtaatt ctaaagtttc catattcaat
     9541 gacctccatt tgttaattgt taggtgataa acctaataat ttagcaccat ttgtataact
     9601 tattttctct ttttcttcat ctgttaaacc cagttcatct aaaaatacac ctaatttttc
     9661 aggctcaata tatggataat cagcagcata aagaattcta tcaataccta cttctttctt
     9721 gactaaatca aactgtggct tcgttaacat gccactcggt gtgatataaa aattattttt
     9781 aaagtaatag cttacagggt ggttcaaatg ttcagcgaat aaagcttcat ccatacgttc
     9841 taagaagaat gggataaact caccccaatg tccaataatc atatttaact ttggataacg
     9901 atcaaaaata ccagataata ctagatgtat tgcatgaatg ccgacatcaa tgtgccaacc
     9961 ataaccaaaa caagcaaatg ttgccgcagt tacttcagga taatttcctt tatagtatga
    10021 ttgataaatg tcactgttaa ctggcgcggg atgtagataa atcggtacgt ctaaattttc
    10081 agctgttttg aaaataatgt catatttgtc ttgatcaaga aaaccatctt gtgcacgtcc
    10141 cataatgagc gcacctttga atcctaaatc attgatgcaa cgttcgaatt ctcgcgctgc
    10201 ggcttcaggc tcattgatag gtaaagttgc aaagcctaca aagcgattgg gatattgtgc
    10261 aatatagttt gccaattgat catttgcttt ttgacataat tcaatggctt tctgaccaac
    10321 caaatttgaa ggagaaccat ttccataaga taagacttga atttgaacgt cttgattatt
    10381 cataaattgg atacgttcat catgatgtga taattcgtcg gcatttgtaa aacctgtctt
    10441 tttttcaagg ccttctaaca ttactttcat cggtacacct ttaggatctg ctgatatcgc
    10501 attcatcgtt tctttttgaa tatcttcaat gacataatgt tcttcaaacg taatactttt
    10561 catttacttc gcctccatat tgtattgcat gtttattgca tctattgcag aagcattttt
    10621 tatatacctc taatttcaat gtttgtaaca taaaattgat ctaccaaggc atctctccat
    10681 cgccattaat aaatgtacct gttgggccat ctgcaccaat cgttgctaat tgaatgattg
    10741 gcttgattcc ttcagaaacg tgtttggaat tattactaaa atcaccaact aaatcagtat
    10801 ttgtagcgcc tggatcagca gcattgattt gcatgttagg taatccttta gcgtattgta
    10861 gcgttagcat tgttactgcc gatttagacg aacaataagc taatgaattc actttagatt
    10921 cagctgtttc ggggtttgta accattccaa atgaacctaa accacttgat acgttgacga
    10981 caacaggttg ttcagatttt tctaagagag ggacgaatgt attcatcatt cgtacgatac
    11041 cgaatacatt cgtttgatat acttcttcaa cgtcacgagg tgtcaatttg gaaggtgctg
    11101 aaaattgacc agatatacct gcattgttaa tgaggatatc aagacggcct tctttttcag
    11161 caatcatgtt ataagcattt ttgactgagt agtcacttgt aacatctaat tgtacataat
    11221 gaacacctaa tttttgtgat gcttgttgtc ctcttacatc attccgagaa cctatataaa
    11281 ctttgtaacc caatgcttta agtgcctctg cacttgcata gcctaaccct ttattgcctc
    11341 ctgtgattaa cacaatttta gtcattacgt cccacctcat ctaaataaat gtttaataaa
    11401 taatttctgt acgcttcaat tgaaatatgg cgatgctcta tttggaaggc aaatacacta
    11461 gttgataatg attgcaacag catatctgtt ttgaacttcg tgtaagtgtc gtcatcgctt
    11521 ttaaataagt cataataaaa atcaaataat tcttgataaa atgcgctttg gtaaaaacgt
    11581 aatttattgt tgcctgcttc aatacattgc agtagtgcct tattatcgat tttaaattgt
    11641 aaaagataat ctaacgacac ttgcataacc tcataattag aatgatagtc atctttaatt
    11701 tgcttaaaat gagtgataaa aatatcaagg tctctttgta tgacgtagta gcataaatcg
    11761 cttttatctt tgaaatgtcg atacaatgtc cccataccga tacctagttc tttagcaata
    11821 cgattcatac taatgttttc aacgccttct tcatcaaaaa gtttgtgcgc tatttcttca
    11881 attcgttgcc tattctcttt tgcatctttt cgcatgatta cacctactta aaattctcta
    11941 aaattgacaa acggataact ctccgtttat tataaaacgt gttaagaaag ttagcaatga
    12001 atttgcaata actattaaat atcataaaag aaaagagtgt tgataatgtc tttaaataaa
    12061 gagcaaagac gcatcacagc tgaagagttg caagcacatt tcgaagcatc taccttatcg
    12121 gttcaaatga ttgctgaaaa actgaatgtc actacagaag atgtggaaaa agtattagct
    12181 atgacagcgc cactaggcat ttttagtcat caattacaac gatttattca tttagtatgg
    12241 gatgtcagag atgtaataaa cgacaatatt aaaggaaatg gacaaacacc agaaccatat
    12301 acgtatttaa aaggtgaaaa agaggactat tggtttttaa gataaaaaga ggagtggatc
    12361 taaaatgaaa gatattttag taataggcgc aacagggaag caaggtaatg cagttgtaaa
    12421 acagttgtta gaggatggat ggtatgtaag tgcattaact agaaataaga ataatcgtaa
    12481 actttcagac ataggacatc cgcatttatc aatcgttgaa ggtgatctga gtgataatgt
    12541 tagtttgcaa tcagcgatga aaggtaagta tggcttatac agtattcagc caatcgttaa
    12601 agatgatgtt agcgaagaat taagacaagg tatgaagata attgaaatag ctgagcaaga
    12661 aaatattcaa catattgtgt atagtactgc gggaggggtt aatcgaaatc gaactggccc
    12721 acactttgaa gtactagcaa aaatagaaaa tagattaatg gaatctaata taaatgcaac
    12781 agttatcaaa ccatcattct ttatggataa ttttttacgc attgctaaag tcgaagatga
    12841 acgtattaca ttaccagaat ttattaatcc gaatataaaa ttcacaatga tttcttctat
    12901 tgatatagct aaaattgcat cgtatatttt tgcacatcca caaagcttta cgcatcaatc
    12961 aatagagatt ggttccgatg aagttacatt aagtgaagca gcaacaattt ttagtgaagt
    13021 gacaggtaaa tctactgtta tagaaggaga atttgttagt ggtgttgcag aaaagcaatg
    13081 gttggaagaa aaaggttatg aagtagactt tgaattaatg gctgaaataa atccaacaag
    13141 attatcatta agtgattggc ttaaagtcca aaactataat aagtaatgaa tggggtgcaa
    13201 tcatgattca atcaatgtgg tttaatttgc atgtgcaaga tttagaaaag agcgcacagt
    13261 tttataaagc gttaggattt aaaataaata gaaacccaca aatgttagat aaaatggtcg
    13321 gcattcaaat aggtcaaaca accgtaattt taatagaaaa caagcatttt caaaatgtaa
    13381 gtcagcaaag ccttaatact gaaccaaatg aagtgatgat ttctctaggt gtgaacacaa
    13441 atgaagaagt tgaccagttg gtgaataaag tgaaagaagc gggtggcaca gtcgttcaag
    13501 aaccaacagt aagccaaggg ttttatggag caatgttcaa agatcttgat ggacaccatt
    13561 ttaatttttt ggtctgctaa aatgaaattc tcaaaagaat aaataaaaag cccatacaga
    13621 gatgaagcgt aatgtgcacc atggtgcgac acattagtgt cttcaatcag tatgggcttt
    13681 gtttatgagg tcagttggca ccttaagtgc aatacctcgt gagcaaatat taattttgaa
    13741 tataaccgta ttgttggcga agatcataaa ctttatctgg tgtgacatcc tgaccgattg
    13801 gatcaaccac ttttattgtg gaaaatgttg ttaatacttg tcctcggatc atgcttaagt
    13861 agtcttgacg caatgcatgt tgttcttgtt tttcagctac agtaagtggt tgtacttttt
    13921 ctttatttgc aagttcatta attctatcta aaattttcat attgtcatat cctccttaga
    13981 atcaagtgtt gatatatttt gagattattt ttatatggta atttatcaac acattaagta
    14041 atgtttttag tttctcggtc ttttgaccag cagatttttg gaattaaaat aagggatttg
    14101 agacacgatt ttactagtag tacgatggca aagtgatgtt attgaagtgt cgtttgatat
    14161 acatgtctgc aatgttgttt tataagacag gatgacgtaa ctgagcaaag aaatgagcat
    14221 tgtcgaatga catcgtgtgt cttattaaaa ttgcaatggg catagcatag gtatattcac
    14281 agtcgatttt cttgatttct acattatcat aatacgtagt gtaaaagtta tacgacagtc
    14341 gatagtcgat tcgcttttta ccacgagcaa ttattatgtg ttgcttcaac gtcgacttta
    14401 atttatttgt aatgtatgtc actacaccca gactactctg attaatattt aatatattat
    14461 gcgtgcgttc aaacccctga ggttcatctg tctcgctcac gtcaatccct cctatcttag
    14521 tatttaaaca acgcatatca actatgcaac atatgttgtt tagacatact atactactat
    14581 gtcgtacgtt tgccaataaa cacgcacata tcatttgttg cctaaacaac atattgcctg
    14641 aagtgttgtt taagtacgca aaaacaaaaa tgcttgtcac taaaatctat atgttttaat
    14701 gacaagctta ttttcattca gttaacgact gctaatatga cattgtagag tataggacgt
    14761 tgatttatgt cctagacacg gctaatcaat cgtaatacgt taaattttat tgtgtgtaca
    14821 agtatgaacc taagtcacat agtgatttca agatgcatga ctaaatacca agttttatta
    14881 aaaatgtgct atatgatgaa ttaaaaatgg aaggttgcga tcgataaaca catcggctgt
    14941 ctcttgactc gtgttaaacc aatgtaatgc taagcctgag ataccagctg ctaaaaaact
    15001 cgcactattt acaatgatat ctcgttgatg ctccggatag ctgttttcta acataatcgc
    15061 aaaaatatct tctaattcat tattaatacg ttgatgtgct ttgttgcaaa atgcttcact
    15121 atttaattcg caagactttg cagcatcttt gatataactc gcaattgaaa tgaaaatatt
    15181 acgcagaacc ttttcattaa tgacattaga aatgctcaaa ttatcattca agtcttttaa
    15241 aatggttaca gataatgtgt agtctaggag gtcttcttta tcagtgaaat gagcgtaaaa
    15301 tgttgcacga tttagcgtag caatatcagt gatatcttta actgtaattt gagataattt
    15361 cttttctcta gaaattttaa gaaaagcatc gacaagcaat tgtttggttc taacgattct
    15421 tggatctatg gatttacttt ccatcatttg ttcactacct aacatctgtt gttttaacac
    15481 ttattatcag tatcttctat tatattgtca attgtgttga tgatatgtaa gtgctgatac
    15541 gctcctaaca aataatgtac ctttttggta agcatttgaa tgtctttctt ctattgtatt
    15601 aatggatttt aaaaatgaag ataaaataaa agtgtattgt aacaatatgt ttgaaaaatt
    15661 gccaatttct ttatctgaaa attcagaatt ttgtgatata ctatttttca tagtcttgag
    15721 ttatagatga tactgctagc ctaaatataa caacatcggc atgcacatct tagaatttaa
    15781 gctagtggca gcttataact taaggctctt tattatttgc atacagatgt atatacataa
    15841 atggaaggat gatattatga catcaattaa aacaattaca cccaaagatt ttatatttag
    15901 agttttatca ggtgtcgcca taggtattgt tgcgggttta gttcctaatg cgatacttgg
    15961 agaaattttt aaatacttta tggattatca tccgattttt aaaacgttat taggcgttgt
    16021 cgttgcaatt caatttacag tacctgcttt gattggtgca ttggttgcca tgaaatttga
    16081 cctatcgcca ttagcaattg ctgttgtggc aagtgcagca tatgtaggaa gcggtgcagc
    16141 gcagtttaaa aatggtgctt ggatgattac gggtattggt gatttaatta atacaatgat
    16201 tacagcagcg attgcagtgt tattcatttt acttattcaa catcgcgtag gtagtatggc
    16261 attgattgta ttcccaacag ttgttggcgg tatttcaggt gcaattggtg ttttaatatt
    16321 gccgtatacg aaaatgatta caacagccat cggtaatatg gttaatggtt ttacagaatt
    16381 acaaccgatt gtcatgagta ttttgatttc aatggtattt agtcttatta ttatttcgcc
    16441 actatcaact gtggccattg cctttgcgat aggtattaca ggtttagcag ctggatcagc
    16501 gtcaattggt atctccgcga ctgaagcagt attgataatt ggtacaagta aagtgaatcg
    16561 tttaggggtg ccgttatccg tcttctttgg aggcgttaaa atgatgatac ctaatatggt
    16621 gaaatatcca attcttatgt taccaatatt aacgacagcc attgtatcag gtttggtatc
    16681 agcattagta ggtattcatg gaacgaaaga gtcagctgga tttggtttta taggaatggt
    16741 aggaccgatt aatgcattta aatttatgga ggttgattct gcttggttga gtgtcttact
    16801 aattgtagta gcgttctttg ttgtaccatt tgtaacagct tggcttgcag atatcattta
    16861 tcgtaaagtg tttcgtttat atactaatga tatttttaaa ttcatgggat aataacagta
    16921 tgttttttga aaatatgaga ccaaaaatta tttaatgtta ttgtgaattg cgagaatgaa
    16981 gttgaacaat ataattgagc tgcagtttat ttgcgctaat aaaagataag acaattcaca
    17041 aaagatgtgt gttttaaaac tttgaatata caacaataat agcaatcaat acatgtgtag
    17101 aaacatattt gttgattgct ttttaattgc gtttaatgta cgaaaataag agaagttaca
    17161 tgtcagatat attacagcta tgtaacaaaa atacaatctg taatattacg aaagctgtaa
    17221 tagttgagct aaatatagta aaaatcttat gtgttaaaga ctatttcaag ggttgattgg
    17281 attctaaagg gcacatattt cacattacag tataaaacag ccatatttca acattacaaa
    17341 taacacttga tattgtaatg ttttgtaaag aaagtgtaat ttactggctg gttttttgtg
    17401 atatagtgag taccgttgaa agaaataaaa gacaatttaa acttgcacta cgacattcaa
    17461 attcaaaata taattactaa aaacataaac ttacaattat tactggagga ttttttacta
    17521 tgaaaaagac aattatggca tcatcattag cagtggcatt aggtgtaaca ggttacgcag
    17581 caggtacagg acatcaagca cacgctgctg aagtaaacgt tgatcaagca cacttagttg
    17641 acttagcgca taatcaccaa gatcaattaa atgcagctcc aatcaaagat ggtgcatatg
    17701 acatccactt tgtaaaagat ggtttccaat ataacttcac ttcaaatggt actacatggt
    17761 catggagcta tgaagcagct aatggtcaaa ctgctggttt ctcaaacgtt gcaggtgcag
    17821 actacactac ttcatacaac caaggttcaa atgtacaatc agtaagctac aatgcacaat
    17881 caagtaactc aaacgttgaa gctgtttcag ctccaactta ccataactac agcacttcaa
    17941 ctacttcaag ttcagtgaga ttaagcaatg gtaatactgc aggtgctact ggttcatcag
    18001 cagctcaaat catggctcaa cgtactggtg tttcagcttc tacatgggct gcaatcatcg
    18061 ctcgtgaatc aaatggtcaa gtaaatgctt acaacccatc aggtgcttca ggtttattcc
    18121 aaactatgcc aggttggggt ccaacaaaca ctgttgacca acaaatcaac gcagctgtta
    18181 aagcatacaa agcacaaggt ttaggtgctt ggggattcta attccttcat aataaataaa
    18241 agtaatgttt agacattaaa tatatagctc ttcccataga aattggggag agttttttta
    18301 ttaattaaaa atacattttt taaaaagtgg atattgaagt tagggaaatt tattggactt
    18361 tattgtatta ctgctaaaat acttgtcttt attaagtgcc aagtggaatg aaacttcatt
    18421 tgaggctgta ctgagtacac tagatacaaa ctaaacgcac attttataat taaattaaat
    18481 gaaaaaggag ataaaaatgg ctataaacat aattgaatat aataggtctt ataaggaaga
    18541 gctaatcgag tttattttat cgattcaaaa aaaatgaatt taatataaaa attgatagag
    18601 atgatcaacc tgatttggag aacatcgaac ataactattt gaataatgga gggcaatttt
    18661 ggcttgcaat aaacaatcat caaaatattg ttggtactat aggtttgatt agactagata
    18721 ataatatgtc tgcacttaaa aaaatgtttg ttgataaagg ttataggaat ttaaaaatag
    18781 gtaaaaaact tttagataaa gttataatga catgtaaaga acaaaatatc gatggtattt
    18841 atttaggaac aattgataaa tttattagtg cacagtattt ttattctaat aatggcttcc
    18901 gtgaaattaa gcgaggagat ttaccaagta gttttcctaa attagatgta gataataggt
    18961 tttattacag aaatttaaaa gattaattcg atgcaataat aattgatatg acaatcttag
    19021 taatattcat tttaaaagtg acacatagcg tttaaatata aagtctgacg agatatatcg
    19081 tttggtttac attagaggat aggctgcatt tcgctttttt ttcaatacat gttaatttat
    19141 tcattatata aattttgaaa aagtatattc gaatagcata tcttaatgat tctgttacag
    19201 taatatgcat aaaatataaa acgaaccttt tttgtaaaaa tataatgaaa ataacagtta
    19261 ttttgctaaa aattcaagaa tctatgataa tctatgttat gtagtaaaat cgacaaattt
    19321 atatgtggtt tgcagtaatg aaatcaactg taataattgt tttctactta ataaaaaatt
    19381 taaaactatc ttaattaagg gttaaatcaa ttaatgaaaa tcagtgcaat attttatttt
    19441 caaaaatatt gtgatatgct acaaaataga gaaaaataaa tggggcgttt attaacatgg
    19501 atacaaaaga ctttaaacgt ttagaaaaaa tgtattcccc gcgatactta cctggattag
    19561 atggattgag ggcattcgca gttataggaa tcattattta tcacttgaat gcacaatggt
    19621 taagtggggg ctttttagga gtagatacat tcttcgttat ttcaggttat ttaataacaa
    19681 gtttgttgat aagtgagtat tatcggacgc aaaaaatcga tttgctagag ttttggaagc
    19741 gacgattgaa acgactcatt ccggcagtgt tatttttaat ctgtgtcgtg cttacgttca
    19801 cactgatatt taaaccggaa ttgattatac aaatgaaacg agatgctatt gcagctatat
    19861 tctatgtttc aaactggtgg tacatttcac agaatgtaga ttattttaac caatttgcta
    19921 ttgaaccact caaacattta tggtctttag ccattgaaga acaattttac ttgcttttcc
    19981 cattggttat cacgttctta ttacatagat ttaaaccgag aaatattatt caaacgctat
    20041 ttattgtatc gttgatttct ttaggactta tgatagtgat tcatttcatc actggagata
    20101 attcacgtgt gtattttggg acagatacac gactgcaaac tttattgctt ggttgtatat
    20161 tagcatttat ttggcctccg tttgctttga aaaaagatat ttctaaaaag attgtcgtat
    20221 cattagatat tatagggata tctggttttg cggttctaat gactttgttc tttatagttg
    20281 gagaccaaga tcaatggatc tataatggag gattttacat tatatcattt gcaactttat
    20341 tcattattgc aattgcggta catccttcta gtttatttgc taaattttta agtatgaaac
    20401 ctttactaat tataggtaaa cgatcatata gcttatactt atggcattat cctatcattg
    20461 tttttgtgaa cagttattac gtacaaggac aaataccggt atacgtttat attatagaaa
    20521 ttttgttaac agcgttaatg gctgaaattt cgtatcgctt tattgaaaca cctatacgta
    20581 aaaaaggatt taaagctttt gcatttttac ctaaaaagaa ggggcaattt gctagaacag
    20641 tgttagttat cctattattg gttccgtcta tcgttgtgct cagtggacag tttgatgcac
    20701 ttggcaaaca acatgaagcc gagaagaaag agaagaagac ggaatttaaa acaacgaaga
    20761 aaaaagtcgt taaaaaagat aagcaagagg ataagcagac agcgaatagc aaagaggata
    20821 ttaaaaagtc atcaccacta ttaattggtg actcggtcat ggtggatatt ggtaatgtct
    20881 ttactaagaa aataccaaat gcacaaattg atggtaaagt tggacggcaa ctcgttgatg
    20941 ctacaccaat tgtgaaatcg caatataaag actatgctaa aaaaggtcaa aaagttgtag
    21001 tagagcttgg tacaaatggg gcatttacga aagatcaatt aaatgaacta ttggatagtt
    21061 ttggaaaagc agacatatat ttagtttcta ttagagtacc tagagattat gaaggtagaa
    21121 taaataaatt aatttatgag gcagctgaaa agcgctctaa tgtacatcta gtcgattggt
    21181 ataaagcttc tgcaggtcat ccggaatact ttgcatatga cggtattcac ttagaatatg
    21241 caggtagtaa agcgctgact gatttgattg taaaaacgat ggaaacacat gctacaaata
    21301 agaaataatt tgatgcacta aacttttgaa atattacatt acttctgata tttattatca
    21361 aaaatgatgt atttcattaa aagtttagtg cttttttatt ttcaaatccc atagtaacgg
    21421 tgcagaaaaa gtgttgtaaa cattctaatt ggtatattac attcaatgaa gctttattag
    21481 gaacagatta cattatgata acaaagcccg caagacacct aatctctgtt atagtttgtt
    21541 ttgtcgcaaa actataaaag ttataattgt ttgcatacta aaaaaataaa aaatataaaa
    21601 tttaaaataa ttgagtcgct aatgactata tgtatagagt gttttgatta ttgggaggat
    21661 atttaattat gaaaaaaatc gttacagcta caatcgctac agcaggactt gccactatcg
    21721 catttgcagg acatgatgca caagccgcag aacaaaataa caatggatat aattctaatg
    21781 acgctcaatc atacagctat acgtatacaa ttgatgcaca aggtaattat cattacactt
    21841 ggacaggaaa ttggaatcca agtcaattaa cgcaaaacaa cacatactac tacaacaact
    21901 acaatactta tagttataac aatgcatctt acaataacta ctataatcat tcatatcaat
    21961 acaataacta tacaaacaat agtcaaacag caacaaataa ctattatact ggtggttcag
    22021 gtgcaagtta tagcacaaca agtaataatg ttcatgtgac tacaactgca gcgccatctt
    22081 caaatggtcg ttcaatttct aatggttatg catcaggaag taacttatat acttcaggac
    22141 aatgtactta ttatgtattt gatcgtgttg gtgggaaaat tggttcaaca tggggtaacg
    22201 caagtaattg ggctaacgca gctgcatcat ctggctatac agtgaacaat acaccaaaag
    22261 ttggtgctat catgcaaaca acacaaggct attacggtca tgttgcttac gttgaaggcg
    22321 ttaacagcaa cggttctgtt cgtgtttcag aaatgaacta tggacatggt gctggtgtgg
    22381 ttacgtctcg tacaatttca gcaaaccaag caggttcata taatttcatt cattaatcaa
    22441 atgtaaatca aatgacgtca atattctcta acatgagagt attggcgttt ttgttttata
    22501 taaatataaa tgagagcggt ttattcactg atctttaggg aactaagtaa taaagtgata
    22561 atttatacta tgtcagtatg attgagagtg attcaattta gatgaaaacc atgaaaaaat
    22621 atattaaaac agcatttttt tgtagtatgt attggttaat tgttcaacta aatatagcaa
    22681 atttaggtac aagaattcct gataagtatt ttcgtcagaa gtacataata tttaaatcat
    22741 ttaactttga gaagcatgga aaattttgga acaaatggtt ttacgtaaga aaatggaaac
    22801 ataagatttt agatggtcat cagcttaatc aaaatatata tgatcagcgt catttaatga
    22861 caatcaatac tgatgaaatt gaaaaaatga ttatagagac aaagagggca gagttgattc
    22921 attggatatc gatacttcca gtcatcatat tcaataaagg ccctcgttta gtaaagtata
    22981 taaatatttt ctatgcaatg atagctaatg ttccaatcat tattgtgcaa cgctataatc
    23041 gaccgagatt aacgcagtta ctacgcatat taaaacgaag aggtgaacgt catgactaaa
    23101 catatcatcg ttattggtgg tggcttaggt gggatttctg cagcaattcg aatggcacaa
    23161 agtggctatt cggtctcatt atatgaacaa aataatcata taggaggcaa agtgaatcgt
    23221 catgaatcag atggctttgg ctttgattta ggtccatcta ttttaacgat gccttatatt
    23281 tttgaaaaat tattcgaata tagcaagaag caaatgtcag actacgttac aatcaagcga
    23341 ttgccacatc aatggcgtag cttttttcca gatggaacga ctatcgattt gtatgaaggt
    23401 attaaagaaa caggtcagca taatgcgata ttgtcgaaac aggatataga ggaactgcaa
    23461 aattatttga attatacaag acgaatcgat cgtattactg aaaaagggta tttcaactat
    23521 ggtttagata cactatctca aattattaaa tttcatgggc cattaaatgc tcttattaat
    23581 tatgattatg tacatactat gcaacaggcc atagacaagc gtatctcgaa tccatacttg
    23641 cgacaaatgt taggctattt tatcaaatat gtaggttctt catcatacga tgcgccagct
    23701 gtattatcta tgttattcca tatgcaacaa gagcaaggcc tttggtatgt agaaggtgga
    23761 atccatcatt tagccaatgc cttggaaaag ctagcgcgtg aagaaggtgt cacaattcat
    23821 acaggtgcac gtgtggacaa tattaaaaca tatcaaagac gtgtgacggg tgtcagatta
    23881 gatacaggtg agtttgtaaa ggcagattat attatttcaa atatggaagt catacctact
    23941 tataaatatt taattcacct tgatactcaa cgattaaaca aattagagag ggaatttgag
    24001 ccggcaagct caggatatgt gatgcattta ggtgttgctt gccaataccc gcaattagca
    24061 catcataatt tcttttttac ggaaaatgct tatctcaatt atcaacaagt ttttcatgaa
    24121 aaggtattgc cagatgatcc gaccatttat ctagtaaata cgaataaaac tgatcacaca
    24181 caagcgccag taggttatga aaatatcaaa gtcttaccac atattccata tattcaagat
    24241 cagcctttta ccactgaaga ttatgcgaag tttagggata aaattttgga taaattagaa
    24301 aaaatgggac ttactgattt aagaaaacac attatttatg aagatgtttg gacaccggag
    24361 gatattgaaa aaaattatcg ttctaatcgt ggtgcaatat atggtgttgt agcagataaa
    24421 aagaaaaaca aaggatttaa atttcctaaa gaaagtcagt attttgaaaa cttgtacttt
    24481 gtaggtggat cagtaaatcc tggtggtggc atgccaatgg ttacattaag tgggcaacaa
    24541 gtcgcagaca aaataaacgc gcgagaagcg aagaatagga agtgatatct atgaaatggt
    24601 tatcacgaat attaacagta atagtgacca tgtctatggc gtgtggtgca ttgatattta
    24661 atcgtagaca tcagctaaag gcgaaaacgc tgaacttcaa tcataaagca ttaacaatta
    24721 ttattccggc tagaaacgaa gaaaaaagaa taggtcattt actacattcg ataatacaac
    24781 agcaagttcc agtagatgtc attgttatga atgacggatc gacagatgaa acagcacgtg
    24841 tagcacgttc atatggtgcg actgtagtag atgttgttga tgatactgac ggcaaatggt
    24901 atgggaaatc acatgcttgt tatcaaggtg tgacgcatgc atgtacgaat cgcattgcct
    24961 ttgtagatgc tgatgtaact ttcttaagga aagatgctgt tgaaacgttg attaatcagt
    25021 atcaattaca aggtgaaaaa ggattgttaa gcgtacagcc ttatcatata acaaagcgtt
    25081 tctacgaagg gttttcagcg atatttaatt taatgacagt cgttggtatg aatgtatttt
    25141 ctaccttaga cgacggtcgg actaaccagc atgcatttgg accggtgaca ttaacaaata
    25201 aagaagatta ttatgcaact ggaggtcata aaagtgcaaa ccgtcatatt attgaaggat
    25261 ttgctttagg aagtgcatat acttcacaat cattgcccgt aacagtttat gaagggtttc
    25321 catttgttgc atttcgcatg tatcaagaag gatttcagtc attacaagaa ggatggacaa
    25381 agcatttgtc aactggggca ggtggcacaa agcctaagat catgacagca attgtgttgt
    25441 ggttgtttgg ttctatagcg agtattttag ggctatgtct tagtttaaaa tatcgccaaa
    25501 tgtctgtaag aaaaatggta gcactttact tgagctatac tacacaattt atttatctgc
    25561 atcgaagggt cggccaattt tctaatttat taatggtatg tcatccattg ttatttatgt
    25621 tttttactaa aattttcatc caatcttgga aacaaacgca tcgttatggt gtagttgaat
    25681 ggaaaggtcg tcaatattct atatctaaag aacaataaat caaggtaatg gcatttcaat
    25741 ataggaggac tagtatgaca atgatggata tgaattttaa atattgtcat aaaatcatga
    25801 agaaacattc aaaaagcttt tcttacgctt ttgacttgtt accagaagat caaagaaaag
    25861 cggtttgggc aatttatgct gtgtgtcgta aaattgatga cagtatagat gtttatggcg
    25921 atattcaatt tttaaatcaa ataaaagaag atatacaatc tattgaaaaa tacccatatg
    25981 aacatcatca ctttcaaagt gatcgtagaa tcatgatggc gcttcagcat gttgcacaac
    26041 ataaaaatat cgcctttcaa tctttttata atctcattga tactgtatat aaagatcaac
    26101 attttacaat gtttgaaacg gacgctgaat tattcggata ttgttatggt gttgctggta
    26161 cagtaggtga agtattgacg ccgattttaa gtgatcatga aacacatcag acatacgatg
    26221 tcgcaagaag acttggtgaa tcgttgcaat tgattaatat attaagagat gtcggtgaag
    26281 attttgacaa tgaacggata tattttagta agcaacgatt aaagcaatat gaagttgata
    26341 ttgctgaagt gtaccaaaat ggtgttaata atcattatat tgacttatgg gaatattatg
    26401 cagctatcgc agaaaaagat tttcaagatg ttatggatca aatcaaagta tttagtattg
    26461 aagcacaacc aatcatagaa ttagcagcac gtatatatat tgaaatactg gacgaagtga
    26521 gacaggctaa ctatacatta catgaacgtg tttttgtgga taagaggaaa aaggcaaagt
    26581 tgtttcatga aataaatagt aaatatcata gaatataggt ggttgaataa tgaagattgc
    26641 agtaattggt gcaggtgtca caggattagc agcggcagcc cgtattgctt ctcaaggtca
    26701 tgaagtgacg atatttgaaa aaaataataa tgtaggcggg cgtatgaatc aattaaagaa
    26761 agacggcttt acatttgata tgggtcccac aattgtcatg atgccagatg tttataaaga
    26821 tgtttttaca gcgtgtggta aaaattatga agattatatt gaattgagac aattacgtta
    26881 tatttacgat gtgtattttg accacgatga tcgtataacg gtgcctacag atttagctga
    26941 attacagcaa atgctagaaa gtatagaacc tggttcaacg catggtttta tgtccttttt
    27001 aacggatgtt tataaaaaat atgaaattgc acgtcgctat ttcttagaaa gaacgtatcg
    27061 caaaccgagt gacttttata atatgacgtc acttgtgcaa ggtgctaagt taaaaacgtt
    27121 aaatcatgca gatcagctaa ttgaacatta tattgataac gaaaagatac aaaagctttt
    27181 agcgtttcaa acgttataca taggaattga tccaaaacga ggcccgtcac tatattcaat
    27241 tattcctatg attgaaatga tgtttggtgt gcattttatt aaaggcggta tgtatggcat
    27301 ggctcaaggg ctagcgcaat taaataaaga cttaggcgtt aatattgaac taaatgctga
    27361 aattgagcaa attattattg atcctaaatt caaacgggcc gatgcgataa aagtgaatgg
    27421 tgacataaga aaatttgata aaattttatg tacggctgat ttccctagtg ttgcggaatc
    27481 attaatgcca gattttgcac ctattaaaaa gtatccacca cataaaattg cagacttaga
    27541 ttactcttgt tcagcatttt taatgtatat cggtatagat attgatgtga cagatcaagt
    27601 gagacttcat aatgttattt tttcagatga ctttagaggc aatattgaag aaatatttga
    27661 gggacgttta tcatatgatc cttctattta tgtgtatgta ccagcggtcg ctgataaatc
    27721 acttgcgcca gaaggcaaaa ctggtattta tgtgctaatg ccgacgccgg aacttaaaac
    27781 aggtagcgga atcgattggt cagatgaagc tttgacgcaa caaataaagg aaattattta
    27841 tcgtaaatta gcaacgattg aagtatttga agatataaaa tcgcatattg tttcagaaac
    27901 aatctttacg ccaaatgatt ttgagcaaac gtatcatgcg aaatttggtt cggcattcgg
    27961 tttaatgcca actttagcgc aaagtaatta ttatcgtcca caaaatgtat cgcgagatta
    28021 taaagattta tattttgcag gtgcaagtac gcatccaggt gcaggcgttc ctattgtctt
    28081 aacgagtgcg aaaataactg tagatgaaat gattaaagat attgagcggg gcgtataagg
    28141 gagtagtcta agagaaagat gtgagaaaag tataagggga acgtaaaagg catagtgaaa
    28201 taagtatgtg cttaagaagt tttatttaac aatgaatgac gcctgttaag gttcacgttt
    28261 gaatacgtac cttgatgggc gttatacttt atataagcat atctaactgg ttgcgcatgc
    28321 ggatatgcat gacagttaaa tcataacgat tgagtgcata ttaatctgaa aattcctaat
    28381 attgacaata atattatacc tacctaaaat gaaaataaat tcattcaata caacgtgtgt
    28441 cataagttct gttgaacata acggtgaatg gcgatgtcgt tgtaaccatc taaatgagag
    28501 tgatacaagt tgttaggatt atgatagtcg cccaggctat gtttaatgat agaggaggaa
    28561 tttgatgatc tctaacaaat tagcaaatat ccctgatagt tatttcggga aaacgatggg
    28621 acgtaaaatc gaacatggac cattgccatt aattaatatg gcagttggaa tccctgatgg
    28681 gcccacgcca caaggtatta ttgatcattt tcaaaaagca ctaactattc ctgaaaatca
    28741 aaaatatggt gcttttcatg gtaaagaagc gttcaagcaa gctattgtag atttttatca
    28801 aagacaatat aatgtgacgt tagataaaga agatgaagta tgcattttat atggtacaaa
    28861 aaatggatta gtggcagtac caacatgtgt tatcaatcca ggagactatg tattactacc
    28921 tgatccaggc tacacggatt atttagcagg tgtactttta gctgatggca agccagtccc
    28981 gcttaattta gaaccgccac attatttgcc agattggtcc aaagttgatt cacaaataat
    29041 agataaaaca aaactcattt atttaacgta tccaaataat ccaactggat cgacagctac
    29101 gaaagaagtt tttgatgaag cgatagctaa atttaaaggt acagacacta aaatcgtgca
    29161 tgattttgct tatggtgctt tcggtttcga cgctaaaaat cctagtatac ttgcttcgga
    29221 aaatgggaaa gatgttgcga ttgaaattta ttccttgtct aaaggttata acatgtcagg
    29281 ttttagagtc ggttttgcag ttggtaataa agatatgatt caagctttga aaaagtacca
    29341 aacgcacaca aatgctggga tgtttggtgc attacaagat gccgcaatat atgctttgaa
    29401 tcattatgat gattttttag aagaacaaag caatgtattt aaaacacgtc gtgaccgatt
    29461 cgaagccatg cttgcaaaag ctgatttgcc atttgttcat gctaaaggtg gcatatatgt
    29521 atggttggaa acaccgcctg gttatgacag cgagcaattt gagcaatttt tagtgcaaga
    29581 gaagtcaata cttgttgctc cagggaaacc atttggagag aatggtaatc gttatgtgag
    29641 aatttcattg gcgttagatg accagaaatt agacgaagct gcaataagat taacagaact
    29701 agcatattta tatgaataat aaagttaagg gtgatgttaa aatgacaaaa attaaaatca
    29761 tgagtgtacg tgatgaagat atgccttata tcaaggcgtg ggcagagaaa catcatgttg
    29821 aggtagatat tactaaagaa gcattaactg atgacaatgt tgaaggtgtc gctggttatg
    29881 atggcttatc gctatcacaa caaataccat tatcagagca tgtttataaa cgattaaatg
    29941 aactaggtat taaacaaatc gcacaacgta gtgctggatt tgatacatat gatttggagt
    30001 tagcaaacaa gtataacttt attgtgtcaa atgtgccgtc atactctccg aattcaattg
    30061 cggagtttgc tgtgaaccag gctattaatg tggttcgtca ttttaatcaa attcaaacga
    30121 aagtgcggga gcatgatttt agatgggaac ctactatttt atcgaaatcc attaaagatt
    30181 taaaagtagc tgttattggt acaggtcgaa ttggccgtgt agtagccgat atatttgcca
    30241 atggttatca aagtgatgtg gtcgcatacg acccgtttcc taatgctaaa attgcaacgt
    30301 atgtcgatta caaagatacg attgaggaag cggttgaagg tgctgatatc gtgacattac
    30361 atgtacctgc aacgaaatat aatcattatc tatttaatgc tgaattattt aaacatttta
    30421 aaaagggcgc agtatttgtc aattgtgcga gaggttcttt agtagatact aaggcgttat
    30481 tagacgcatt agacaatggt gtgattaaag gtgcagcact tgatacgtat gaatttgaac
    30541 gcaaactttt cccaagtgat caaaggggca aaacactgaa cgatccattg ttagaatcgt
    30601 tgattgacag ggaagatgtc atattaacac cacatattgc gttttatact gaagctgcag
    30661 ttaaaaatct aattgtcgat gcattagatg caacattaga tgtattgcag actggagata
    30721 ctaggttacg agtaaattaa aaatcgaact gatgagataa tttggattgt tgggattctc
    30781 atcagttcga ttttttaatt tggtgttgat gacgttgaat attgcctaat taaacgacat
    30841 cgtaaccttg atcttcaatt gcgtctttca tttgagatac agcaacttta ctgtcatcat
    30901 attgaacact tacttgacca ttttcaaggt taacgtcagc tgaagtgaca ccgtcaatat
    30961 tatttaatgc agattcaaca gcacttttgc agtgaccaca gctcatacct tcaacattta
    31021 aaatttcttg tgacatagca ttcacctcct tttagagcca cttattattt ataataatta
    31081 gtggctctta tgcagttgga gcgaagatcc aactgtaaac cgcgacatta tagagccaat
    31141 cactagccta tctcagaatc accaacgaat ccttcattat taaggaatct aggcatcttt
    31201 acggcgtggt tctaatcgca tctttttcaa tctaagtgcg tttgtgacaa cacttactga
    31261 acttagtgcc attgcagcac cagcaaccca tggcgcaagt aagcccaatg cagctatagg
    31321 gataccggca atattatagc cgaatgccca aaatagattt tgacgaatat tacgaatggt
    31381 tgctttactt gcataaatgg ctttaggaat aagcatcaag tcgccaccaa gaatagtaat
    31441 atcagctgct tcaatggcaa cttctgtacc tgtaccaatg gcgataccga tatcagcttt
    31501 aactaatgca ggtgcatcat ttacaccgtc accaaccatc gcaaccttct taccttgttg
    31561 ctgtagtttc gcaatttgtg cagctttttc ttctggtaaa atatctgcaa taacagtatc
    31621 tatgcctact tgttttgcaa tggcttgagc agtgttttta ttatcgccag ttaacatggc
    31681 aacttcaatg cccatatcat gcaattgttt tatagcatct ttggcatgat ctttgacagt
    31741 atctgccact gcgatgatac cagttaatga ataattaaca gcaatgagca tagcagtttt
    31801 accatctcgt tcataatgtg ttaaatcatc agaaatatgc ttaggcaagc taatatcatt
    31861 gtcagccatt aatttacggt taccaaccaa tatatagtga tgatcaatcg ttgcttcaat
    31921 accatggcca ggtactgctt taaatgttgt tgtctcagtt aatattaatt gcttttcttt
    31981 tgcataattg acaatggctt ctgccaatgg gtgttcagaa tctttttcag cagtagcaag
    32041 tagttgtagc gtttgattgt caccatgata atctgtcacg actggacgac catttgtaat
    32101 ggtacccgtc ttatctaaaa cgatggtatc aatttgatgt gtgcgttcaa caaactcgcc
    32161 acctttaaat aaaataccat tttcagcagc gcgaccagta cctaccataa tagaagttgg
    32221 tgtagcaagt cccaatgcgc atggacaagc aatgacgaga acggaaatac tcgcaacaag
    32281 tgcaggttca aatgtacctg gtgtaactaa agtaatccac acgataaatg ttaatagtgc
    32341 gataccaaca acgataggaa cgaaataacc agaaataata tctgccaatc gttgaatcgg
    32401 cgctttagaa ctttgagctt cttcgacaac tttaataata tttgccaacg cagtgtcccc
    32461 gccaactttt gttgctgtca tagtaatagt accgtttttg ttcatcgttg aaccaattac
    32521 agtatcatca acattcttct caacagggat agattcacct gttaacatag attcgtcgat
    32581 ggcagtcata cctttaataa ttttgccatc aacaggtatc ttttcacctg gtttaacgat
    32641 aagtgtatct ccaacatgta cttcatttag aggaatcatc acttcattac catcttttaa
    32701 aatgcgtgct tctttagctt gtaaacttaa taattcgcca agcgcattgg ttgtttgaga
    32761 cttcgctcta gcttctaaat acttaccgaa taagattaag gtaattagta cggcgcttgt
    32821 ttcaaagtat aaatgcggtt gcgttgttga gccatttagc caacgaacca tttcataaat
    32881 actgtaaaaa tatgctgcac ttgtaccaac agcaacaagt acatccatat tggcgccacc
    32941 atttcttaag tttttataag cacctacata aaattgccat ccaataataa attgtacagg
    33001 tgtagctaaa ataaattgga accatggatt cgtaaatagt gctggtatat gcatattgaa
    33061 aagatgtaca aacattaaca ttaatagtgg taaagataat actgctgata tgataagctt
    33121 aatcaattta tgttgtagcg cttcagcttt gcgtgacgtt tgatctttat tgttatcttt
    33181 aatagacgcg tcataaccta atttttgaat gcgagtgaca agtttatcag catctgtttc
    33241 ttcaggataa tagtcaactt tagcttgctc tgttgttaaa ttgaccgttg cattttgaac
    33301 gccgtccatt ttatttaaca ctttttcaat acggcttgag catgcagcac aagtcatacc
    33361 tgtaatgtct aattcgacag tttctacagc gacaccgtaa cctaaatgtt gaatcgtatt
    33421 aataaattct tggacatcat gttgatcagg gttatactca acagttgctt tctctgtagt
    33481 taaattcact tgggcattaa cgtcatcaag tttattcagt ttcttttcga tacgatttga
    33541 acatgcggca caagtcatac cagtgatatc taatgttgtt tttttcgtat tagccacatt
    33601 attgcctccc tgtgattcat tgttacacgt ctaatgtacc ccctataggt atataagtca
    33661 atcgttgcgt tttactttta ccaattttaa aaagaaaaag agagattgcc gtgcaaacat
    33721 tgcacaacaa tctctctact tagcatctat ataaaagaag tcgtaattaa tattaaagga
    33781 tgtatgaatg aaaggtaaaa cttaaatgac ttctggattt gcaattctta ggtgacttaa
    33841 aagaaatcag atgggttaaa ttctttcgca tgtaaatttt taaaattatt aagatggatg
    33901 actttatcat catctttgtt gtctgacaac tgacgactcg tgtcgtttgt catttcgtaa
    33961 tcttgtatcg ttttgaccaa acctagaatg attaagatag caactgtgcc tgtaattaat
    34021 attctcatcg ctatcatact cctttatatt tctcttactc acattgtacg ttaatagatt
    34081 aaaaaatgaa tgcgtctgta gtattgaatt tatctactac tttagcagta tttgatgcga
    34141 gaattgtata gataagggtg ataaggtcat gatttcataa tgttatagcg tattagttgc
    34201 ataggcgaag gtttatttga ttaactttta aacaagtaaa taagttatta ggagttgaaa
    34261 ggtgtaatga ctgaaaaaga aaaaatgtta gcagaaaaat ggtacgatgc aaactttgat
    34321 caatacttaa tcaatgaacg tgcacgagcg aaagatattt gctttgaatt aaatcataca
    34381 agaccgagtg ctacaaataa aagaaaggaa ttaattgatc aattatttca aacaacaaca
    34441 gacaatgtaa gtatttcgat tccttttgat acagattatg gttggaacgt taaactagga
    34501 aaaaatgtct atgtaaacac caattgttac tttatggatg gtggacagat tacaattggc
    34561 gataatgttt ttataggacc taattgtgga ttctacacag cgacacatcc acttaatttc
    34621 catcatagaa atgaaggttt tgaaaaagcc ggacccattc atattggcag taatacttgg
    34681 tttggcggtc atgtagccgt gttaccaggg gtgacgattg gagaaggcag tgtgattggt
    34741 gctggtagtg ttgtcactaa agatattccg ccacacagct tagcggttgg aaacccttgt
    34801 aaagtcgttc gtaaaattga taatgaccta ccatccgaaa cattaaatga cgaaacgata
    34861 aagtagcagt tgcgacccat ataaatttta aaaagtaacc aatcaatttc aaaatacttg
    34921 agaataagtg ataaaacata aaaatgaaag cgattgcaaa actaacctta atcatagtaa
    34981 aatgagttta aatgatgtta tatcattaaa tcaaagggtt aaaccacttt caaaatttat
    35041 agggggtaag tgtacatggt agtagaattt aaaaatgaac ctggttacga tttttcagtc
    35101 caagaaaatg tggatatgtt taagaaagca ttgaaagacg tggaaaaaga attaggtcaa
    35161 gacataccat tagtgattaa tggagagaag attttcaaag atgacaaaat taaatcaatc
    35221 aatcctgcag acacatcaca agtgattgca aatgcatcaa aggcaacaaa acaagatgtt
    35281 gaagatgcgt ttaaagcagc aaacgaagct tataaatctt ggaaaacatg gtcagcaaat
    35341 gatcgtgcag agcttatgtt acgtgtttcg gcaattattc gtcgtcgaaa agcggaaatt
    35401 gcagcaatta tggtttatga agcaggtaag ccatgggatg aagcagttgg tgatgcagct
    35461 gaaggtattg attttattga atactatgca cgttcaatga tggacttggc tcaaggtaag
    35521 ccagtacttg atcgtgaagg tgaacacaat aaatatttct ataaatcaat tggtacaggt
    35581 gttacaattc cgccgtggaa cttcccgttt gcgattatgg ctggtacaac tttagcacca
    35641 gttgtggcag gtaatacagt gttattgaaa cctgctgagg atacacctta tatcgcttat
    35701 aaattaatgg aaattttaga agaagcagga ttaccaaaag gcgtagttaa ctttgttcct
    35761 ggtgatccga aagaaattgg tgattactta gttgatcata aagatacgca tttcgttaca
    35821 tttacaggat cacgtgcgac aggtactaga atttatgaac gtagtgccgt tgttcaagaa
    35881 ggtcaaaact tcttgaaacg tgttatcgct gaaatgggtg gtaaagatgc cattgtagtt
    35941 gatgaaaata ttgatacaga tatggcagca gaagctattg taacgtcagc atttggtttt
    36001 tctggtcaaa aatgttcagc atgttcacgt gcaattgtgc ataaagacgt ttatgacgaa
    36061 gtattagaga aatcaatcaa attaacaaaa gaattaacgt tgggcaatac tgtagacaat
    36121 acgtatatgg gcccagttat taataaaaaa caatttgata agattaaaaa ttatattgag
    36181 attggtaaag aagaaggtaa attagaacaa ggtggcggca ctgatgattc aaaaggttac
    36241 tttgtagaac caacaatcat ttcaggactt aaatctaaag atagaatcat gcaagaagaa
    36301 atttttggtc cagttgttgg ctttgtgaaa gttaacgatt ttgatgaagc aattgaggtt
    36361 gcaaatgata cagattacgg tttaactggt gcagtgatta caaataatcg cgagcattgg
    36421 attaaagcgg taaacgaatt tgatgtaggc aatttatacc ttaacagagg ctgtacttca
    36481 gcagttgttg gataccatcc atttggtggt ttcaaaatgt caggtacgga cgctaaaaca
    36541 ggtagtccag actacttatt acacttctta gaacaaaagg ttgtttcaga aatgttctaa
    36601 tgcttcataa tgaacaaggg ccatccattg cggttggctc tttttattta taactttaaa
    36661 aattaagtaa tagcaaccat atttttagaa aaggattata ggtataagaa tgtgagatac
    36721 gttatttcaa taataatggg aattgtatta gcaatttggt catttaaaca actgagtcaa
    36781 agtcatttag atagcggatt tatttttttc ttcatagttt atgtgttgtg tatcagttgt
    36841 tttaatagcg ataagcacga taaaaataaa aaacgatagt agtacacgaa ttcattaacg
    36901 tgtacactat cgtttttatt atgaatgttg gtaacgctca tgagtttctc atctactatt
    36961 taatcttaat attgattaat ctatggaaaa tgttatcaat atcttcgcca ggccaatcaa
    37021 ttttttcatc ttttatccaa tgtataaaac cttcaatcaa cgagtcataa atataaaata
    37081 tgagattatc tgggatgtcg ctgtcaatat cgatacggtc tctattattt ttgatatcgt
    37141 tatgcataat tttaatgcag acatttttta atactttatt aaattcagcg tcttcttctt
    37201 ggaattctgc gatttctctc aaatcctctt tattattaat cgtatcactc atcgtttgga
    37261 atggatgatt aagacggtca ctgatatctc tagcaaagta gtctttagtc aattgattga
    37321 acaagtactc tagaagatca tatttatcat aaaaatgttt ataaaatgtt gtacggtgta
    37381 cgagtgcgtt gtcgcaaatt tgattgacag taatcgtttg gaataattgc tgttctaaca
    37441 attgtagcaa gctactcgac aatgcttttt ttgtctttat aactcgtaaa tcagtctctt
    37501 tcatctacat ttctcctttg tgtgtagttt aatatacaaa attttccaat tgattcttta
    37561 agaggtcggg gatagatact ataatcgaac aataatctac actgtatatt taaactataa
    37621 cgttgatttt aaaaggaggc aatatacttt ggcaaaattt ttatataaga tggggacatt
    37681 catcgcaaaa cataaatggt ccgcggtcat tgcatggata gtgattgtag ctgcaatatt
    37741 gataccactt gctacaaatg caccgaaatt tgacaatgat attaaaatga caggattaga
    37801 gtcgttagat acgaataaaa agattgaaaa acattttaat caagatagtg aaaaggcaca
    37861 aatacgtgtt gtctttaaga cgactaaaga tgatggtatt gtgcaaccga acattactaa
    37921 agatattaag aaaacgttag atgatattaa aaaagatgac aagcacattg ataaaatatc
    37981 agatccttat gaaaataagc aaattagtaa agataaaaca actgcatttg cagatatcac
    38041 atatgacgtt agtcaaacat ctttaaaaga tggctctaga gataatgtta aaagtcattt
    38101 gaaagactta cgtgacaatc ataatgtaca aacagagtta actggtacag gaatgacatc
    38161 tacagaagta ggcggtaatt ctgaactagt tggtattatt gttgcatttg ttgtactgtt
    38221 aatcacattc ggttcagtca ttgctgcagg attaccaatt ataagtgcat tgattggtct
    38281 agcctctggt gtaggtatta tcagtttgtt aacttatgca ttcgatatac caaatgtcac
    38341 actgacatta gccgtaatga ttggactagc agttggtatc gattatgcat tatttatttt
    38401 attcagatat cgacaagtta tgaaaactga aacagattac attaaaggca ttggtcttgc
    38461 aataggaaca gcaggtagtg cagttgtttt tgctggtgta acagttgtaa ttgcagtatg
    38521 tggtttatca ttagtaggca ttgacttctt agcggtaatg ggatttgctt cggcaattag
    38581 tgttgttttt gcagtactta gcgctttaac tttattacct gcattaataa gcattttcca
    38641 taaacgcatt aaagtgaata aattacagtc taaattcaaa aaagatattg atacgccatg
    38701 gtctaaattt attacaggaa atgcacttgc ggcagtatta ctaggtttaa tcatattagt
    38761 agcagctgca ataccagtta gtcatatgcg acttggtatt ccagatgacg gtgttaaacc
    38821 agcagattct acacagaaaa aagcttacga tattatctcg gataaatttg gtgaaggatt
    38881 taatggccaa ataccgatgt tgataaatgt gaaagataaa aaggatgatc cacaaggatt
    38941 acaacaagat ttacagtctg tatataaaga tattaaagat aagaaaaatg tagatatcgt
    39001 tacgccacca caaatgagta aagataatga ttacgctttg atggtcgtga tacctaagca
    39061 agggcctaat gcagagagta caaatgattt agtgcatgat ttacgagatt atcataaaga
    39121 tgcacaagat aaatatgggt tcaaaacgga gatttcggga caaagtgtca ttaatattga
    39181 tatgtctaaa aagttaaatg aggcgatacc tttatttgcc tcagtcattg ttgtattagc
    39241 atttttctta ttaatgattg tcttccgttc aattctaata ccattaaaag cagttttagg
    39301 attcgtcttg tcattaatgg caacattagg atttacaaca ttagtaatgc aagatggttt
    39361 tatgaaaggt ctgtttggta ttgaaacgac aggaccaatg ctagcatttt taccggttat
    39421 tactataggt atcttatttg gattagcgat ggattatgaa gtcttcctta tgtcgcgaat
    39481 tcatgaagaa tatagtaaaa caggggataa tgattattct atcaaagtag gattgaaaga
    39541 aagcggtcct gttattgtag cagctgcatt aattatgttt agtgtcttct ttgcgtttgt
    39601 attccaagaa gatgtcatga ttaaatcaat gggtatggca ttagcgtttg gtgtactctt
    39661 tgatgccttc gtagtcagaa tgatgttaat tcctgcatta acgaaattgt ttggaaaagg
    39721 ttcgtggtat ttaccagcat ggttgaatcg cattattcca cgagttgata ttgaaggaca
    39781 tgcacttgaa aaatataaaa cggttgaatc acaggaatct gaagctaaag atagtaagga
    39841 aacatacgac acaaccttta aagtatatcc acaaggtgca acaaatgtat cgaaacatca
    39901 agatgcacat gggcaagatg atgcacattc aatagtacta gatgataaaa cgatggcatt
    39961 gtaccaagaa gtgaaacagc aatcagcaag ttcattgttc ttatatgatg ctttaataga
    40021 ttatcaaaac aagcatcaac taaattcaaa aaaacaagca acgaatatag aacagttgaa
    40081 taaaaatatt gaaaagctca atcaattgtt agaaaaaaat ctacgaaata aatcataaaa
    40141 taacatgtac atgcctccgc tttaaatgtc tatcaatgac atttgaagcg gaggtttttt
    40201 gcatggaagt gattatacaa aacatttttg caatgtcaca aattttacat gaaaaatgaa
    40261 aattatttaa ataaagtatt gcgagctaca attttaaggg ttatgatttt taagtgataa
    40321 tgattattat ttctaatagg agagcgaaat agtatgttaa acattaaaaa tggtgaaatg
    40381 aataaggctt ataaaataaa gcgaatggat attgctaatg agaatatgtt gtatcgtcta
    40441 agtgcctttg ggttaacaga tgacgctatc ataacgatta aacaaaaatg tttatttaaa
    40501 gggccatgta ttattgaagt aaacggacaa cagttaagta ttagacattg cgatgcttgt
    40561 tctattgcat tagaagaata ggagccggac gaatggaaaa ttattgtatt ttaggaaatc
    40621 ctaatgttgg taaaacatct ttgtttaacg ctttaactgg ttcgtatgaa tatataggca
    40681 actggagtgg cgtaacggta gagaaaaaag tagggaaact taaagaaaat gtaggacaat
    40741 taattgattt accaggtaca tatgatttat cgccgatttc taaagatgaa actgtagtga
    40801 cagactattt attaaacgat tcattttcag gaatcattaa cattgttgat gcgagtcaac
    40861 taaaaagaaa tatgcaatta acagtacaat tgttagaact taatcaacca atatacatcg
    40921 gattaaatat gattgatgta gctacaaagc gcgggataaa aatcgattat cataaattga
    40981 tgaaaaagtt aaaaacacct atttttccag ttgtggcacg tacaggcaaa ggaacaaaat
    41041 atttacttgg cgaaataaag catctagggg aagggtatca accgcatttt aaaatcaatt
    41101 atggtgagaa gattgaagag acaattaaaa atatgtgtca aataataatg acagagacat
    41161 ctcatgataa gtatcaagct aggtttattg cgattcaatt cttattaaat aacatgcaaa
    41221 ttgcaaacga acttaattca gaggttgtaa ataaattgtc gagtttacgt gatcaagttg
    41281 ctgaacaagt aggggctgta tctgttcgtc gagagatgga gcgtatacgc aatcattata
    41341 ttgagacgtt attgcaagac gtggtaacgt atccagatga agataagcaa tatttcagct
    41401 ctagaataga caagatatta acacataaat atatagggat gcctatcttt ctagcaatta
    41461 tgtggttgat ctttcaaact acatttacat ggattggcac acctttgtcg gatcaactcg
    41521 atgcatttat aggtggtaca tttacagata gtgtgaagac gattatgaat tatcttggtg
    41581 ttataccatt tttacaagat ttaattacag atggcattat tgctggtgta ggatcagtat
    41641 tagtctttgt accacaaatt gttgtgctct ttttctttat atctttatta gaagattcgg
    41701 gttatatggc acgtattgcg gttttaatgg atcgtattat ggaatcgttc ggtttaagcg
    41761 ggaagtcttt tataccaatg attatcggat ttggttgtaa tgtaccgagt attatggctg
    41821 cgcgcagtat tgaaaatgaa aaagagcgtt taacaacgat attaattgca ccattcatgt
    41881 catgctctgc tagactgcca gtgtatgcgt tgttcgtagg tatctttttc aaagagaatc
    41941 aatctttagt agtattaagc ctgtatgttt taggtattat tatggcattt ttagtcagta
    42001 cagtacttac taagacgatt ttaaaaaatg ataatgcaat ctttattgtg gaattgccaa
    42061 cataccgtgt gccttccatt aaaacgttgt ggcgtagtac ttgggaaaag gctaagggct
    42121 ttgttcgtaa agcaggtaca tttatttttg gcggctcagt agtcatttgg ctattaagtt
    42181 atgtaggacc acatggtatc aatgtgaata tcaatcaaag ttttttacat atggttggta
    42241 gtttctttgg tatgttagtg caaccgctcg gatttggaac atggcaagca ggtgctacgt
    42301 tagtgccagg atttttagca aaagaagtta tcgtaagttc gatggcgatt atctattcat
    42361 caggtgatgc aggccttgtt aatgtgattc aaaatcaatt cacaccgcta tctgcgtatg
    42421 catttatgat atttatacta ttgtatattc cttgtgtttc tacagtagca gcaatccgta
    42481 aagagactta ttcatggaaa tggacggcac ttgcagttgc ctatcctcta gtcactgcat
    42541 atgtcttaac attcattttt tatcaagtcg gacatttatt tgtttaagga gtggttaaag
    42601 tgtcagtcat tattaacatt ttaatttttt tagcaatttt cggatatgcc ttatatacac
    42661 tagtaaaatt tttcaagcgt tcaaaacaag gaaaatgtgg tacatgtgac attaatcgtg
    42721 attgttgtgg aacagaacag cacacagcga atcattttcc agggaaataa ttaaaaaaat
    42781 gagcacaggc aagggcgacc tgatgctcat ttttgatgcg cgatatgtta aagtgtcgtt
    42841 atttaccttt tacgactaat tcgttatcct caacatctat atccacatgt ttaacgtctg
    42901 tatgatctaa atagtaatct gtaattttgt cacgtacttg ctgttcaaca atacgtctta
    42961 atggacgtgc acctaattct tcatcatagc cttcttcaat taaccaatct ttcgcatctt
    43021 gagaaacgtc catcgtaata ccttttttgt ctaatgtaac ttgtacatcg tctaataata
    43081 agttgacgat atcttgcaat gcatctttat ctaaatgtaa gaattcaacg atgccgttga
    43141 agcggttaag gaattcaggg cggaagaatt ttttcatttc gtgcataata tctttttctt
    43201 cagcgtcatt gccattgcca aagccagcat ttgatgtaca aataataatt gtatttttaa
    43261 agttgatgac attaccttga ccatcagtca aattaccatc atccattact tgtaataaca
    43321 atgttaaaat ttgtggattt gctttttcga tttcatcaaa tagaatgact gagtatggat
    43381 tacggcgtac tttttcagtt aacgtatttg aattgtcatc ataaccaaca taaccagcag
    43441 ttgtaccaat catttttgaa acagctgttg tgtcactata ttcactcata tcaagtcgaa
    43501 tcagtgcatc tttattacca aataaatcaa ttgctaattg tttagcaagc tctgttttac
    43561 caacaccagt agggccaaca aataggaaac tgccaattgg acggttgccg tcatcaaacc
    43621 cagcacgatt acggcgaatt gcacgtgaaa ccatttctac agcttgatct tgacctatga
    43681 ttttacttct taaacgatta gaaatatttt ttaaacgttc aatatcgtta tcatccattt
    43741 gagaaactgg aataccagtt aatcgttgaa tagtatctga aatatcatga actgtagcaa
    43801 cagcagtatg ttcaccatta ctattttcta atttatcttg taatgatttg atttcatttt
    43861 gaatgtcgtc agctttttta tattcttcag cacttactgc tttacgttta tcattttcta
    43921 attcagaaat tcgtttttca gtttcaactt tatcgacagc tggactttgc gcagataaat
    43981 gtgctgctgt aatatctaac acatcgattg ctttatctgg taataatcgt tgtggaatat
    44041 attgaattga taagtcaaca catgctttta atacgtcatc tggtaatttt acttgatggt
    44101 gttcttcgaa tttttcgcga atacctttta aaatttcaac agtatcttta gcgcttggtt
    44161 cattaacaag cacttcatta aatctgcgcg ttaatgcagc atctttaaga atattgtttc
    44221 gatattcatc ttgtgttgtt gcaccaataa tagaaatctc accacgactt aatgcaggtt
    44281 tcaaaatatc agataaccct ttgctacctg aatcacttcc tgtggcacct gaaccgataa
    44341 tttgatggat ttcatcaaag aatagtacgg cattttgtga agatttaaca ccttcgatta
    44401 atttttgaat attttcttca aaagcaccac gatattgcgt tccagcttct aatgatgaaa
    44461 tgtctacaga aataatttct ttgtctttga ttgctgctgg tacatttcct tcaacgattg
    44521 cctgtgctaa accttcaaca atcgcagttt taccaacacc agcttctcca actaatatag
    44581 gattgttttt agttcgtcta cttaaaactt cagcagtttc ttgaatttct ttatcacgac
    44641 caatgactgg atctaataaa ccgtcacgtg cttcttgcgt aaggttacga ccaatttgtt
    44701 ctaaataatt gccgttgcca ccttgttgcc cttgttgtct ttgtgctgct tgttgaaaag
    44761 cttgcgcact ttgttcagca gagtgattgc caccttgttg tgtgagttgc gctagttctt
    44821 caggtgaaac ttctttacca ttaatatagt actttttgtt tccgacttga tttgaacctt
    44881 gcatatcttt catcattctt cgaaaaattg aatcaaagtc gctattgaaa aaaccgttat
    44941 tcatataaca tcacctctaa atttttttga actaaacaaa aaagaaatta cttttgaagc
    45001 gttatgcaaa ctatatacaa ttgcaattgt acttttccac taatagtagg tgataaaaca
    45061 gaatattttt aaaactttat gaa
//
LOCUS       DALAGF010000009        44622 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.009, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000009 DALAGF010000000
VERSION     DALAGF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 44622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44622
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.009"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..849
                     /locus_tag="O2692_000472"
     CDS             <1..849
                     /locus_tag="O2692_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4298119.1"
                     /translation="MKLMPGSPFNDAKLNAEQKEILNEKYGLNDPVATQYLHYLKNVV
                     TGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLTAMFIGVILGLILGVAAATKQNSWVDY
                     TTTVISVIAVSVPSFVLAVLLQYVFAVKLRWFPVAGWEGFSTAVLPSLALSAAVLATV
                     ARYIRAEMIEVLSSDYILLARAKGNSTMRVLFGHALRNALIPIITIIVPMLASILTGT
                     LTIENIFGVPGLGDQFVRSITTNDFSVIMAITLLFSTLFIVSIFIVDILYGVIDPRIR
                     VQGGKK"
     gene            849..1919
                     /locus_tag="O2692_000473"
     CDS             849..1919
                     /locus_tag="O2692_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4298120.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            1936..3018
                     /locus_tag="O2692_000474"
     CDS             1936..3018
                     /locus_tag="O2692_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298121.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            3008..3949
                     /locus_tag="O2692_000475"
     CDS             3008..3949
                     /locus_tag="O2692_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ4298122.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPNHFVFSTEEEAAQLRENKLVTQN"
     gene            3968..5623
                     /locus_tag="O2692_000476"
     CDS             3968..5623
                     /locus_tag="O2692_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCZ4298123.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            5835..7550
                     /locus_tag="O2692_000477"
     CDS             5835..7550
                     /locus_tag="O2692_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ4298124.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVAN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     EKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
     gene            7601..8587
                     /locus_tag="O2692_000478"
     CDS             7601..8587
                     /locus_tag="O2692_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298125.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLPDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANKLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            8590..9570
                     /locus_tag="O2692_000479"
     CDS             8590..9570
                     /locus_tag="O2692_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298126.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAQDMCKKSMPELKDIGNEHQVACFYVDKVGDLND"
     gene            9563..10525
                     /locus_tag="O2692_000480"
     CDS             9563..10525
                     /locus_tag="O2692_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4298127.1"
                     /translation="MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGLLSTAGYIQYLRNDIIENSKKDY
                     VLTARSKGLSMNKIYNKHILRNSLIPIITFLGADIVSILGGAVITETIFSYNGIGKLF
                     LESVIGQDYPLMMALTLFFSFLGLLGNLISDITYGFIDPRIRSN"
     gene            10537..11418
                     /locus_tag="O2692_000481"
     CDS             10537..11418
                     /locus_tag="O2692_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4298128.1"
                     /translation="MQNKSKSPFKIAFSKFIHNKIAMLSVIFLLIITIVSIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGIKDAFNPRGRR"
     gene            complement(11460..12449)
                     /gene="trpS"
                     /locus_tag="O2692_000482"
     CDS             complement(11460..12449)
                     /gene="trpS"
                     /locus_tag="O2692_000482"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCZ4298129.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            12744..13139
                     /gene="spxA"
                     /locus_tag="O2692_000483"
     CDS             12744..13139
                     /gene="spxA"
                     /locus_tag="O2692_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCZ4298130.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
     gene            13510..14229
                     /gene="mecA"
                     /locus_tag="O2692_000484"
     CDS             13510..14229
                     /gene="mecA"
                     /locus_tag="O2692_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCZ4298131.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVYFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
     gene            14350..15335
                     /locus_tag="O2692_000485"
                     /pseudo
     CDS             14350..15335
                     /locus_tag="O2692_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
     gene            15383..17191
                     /gene="pepF"
                     /locus_tag="O2692_000486"
     CDS             15383..17191
                     /gene="pepF"
                     /locus_tag="O2692_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCZ4298132.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLETDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(17651..18457)
                     /gene="yjbH"
                     /locus_tag="O2692_000487"
     CDS             complement(17651..18457)
                     /gene="yjbH"
                     /locus_tag="O2692_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HCZ4298133.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(18480..18845)
                     /locus_tag="O2692_000488"
     CDS             complement(18480..18845)
                     /locus_tag="O2692_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HCZ4298134.1"
                     /translation="MTTTPYDIIGKEALYDMIHYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHIDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            complement(18949..19542)
                     /locus_tag="O2692_000489"
     CDS             complement(18949..19542)
                     /locus_tag="O2692_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCZ4298135.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            19728..20075
                     /locus_tag="O2692_000490"
     CDS             19728..20075
                     /locus_tag="O2692_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298136.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            20092..20727
                     /locus_tag="O2692_000491"
     CDS             20092..20727
                     /locus_tag="O2692_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCZ4298137.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            20744..21553
                     /locus_tag="O2692_000492"
     CDS             20744..21553
                     /locus_tag="O2692_000492"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCZ4298138.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSNEER"
     gene            21550..22404
                     /locus_tag="O2692_000493"
     CDS             21550..22404
                     /locus_tag="O2692_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCZ4298139.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMTNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDRIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            22425..23810
                     /gene="mgtE"
                     /locus_tag="O2692_000494"
     CDS             22425..23810
                     /gene="mgtE"
                     /locus_tag="O2692_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCZ4298140.1"
                     /translation="MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            23820..25664
                     /locus_tag="O2692_000495"
     CDS             23820..25664
                     /locus_tag="O2692_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HCZ4298141.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            25942..26712
                     /gene="fabI"
                     /locus_tag="O2692_000496"
     CDS             25942..26712
                     /gene="fabI"
                     /locus_tag="O2692_000496"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCZ4298142.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(26907..27992)
                     /locus_tag="O2692_000497"
     CDS             complement(26907..27992)
                     /locus_tag="O2692_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCZ4298143.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
     gene            28334..29902
                     /locus_tag="O2692_000498"
     CDS             28334..29902
                     /locus_tag="O2692_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCZ4298144.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            30044..30802
                     /locus_tag="O2692_000499"
     CDS             30044..30802
                     /locus_tag="O2692_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCZ4298145.1"
                     /translation="MSEFKTGKINKHVLYSNILNRDVTLSIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDEK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFDGGHQWKDWKPLLSDILLYFLSKNTDDQLYE"
     gene            30996..31505
                     /locus_tag="O2692_000500"
     CDS             30996..31505
                     /locus_tag="O2692_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HCZ4298146.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIK
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(31618..32808)
                     /locus_tag="O2692_000501"
     CDS             complement(31618..32808)
                     /locus_tag="O2692_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4298147.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHILWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIVNR
                     EKAK"
     gene            complement(32786..33961)
                     /locus_tag="O2692_000502"
     CDS             complement(32786..33961)
                     /locus_tag="O2692_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HCZ4298148.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            34394..35875
                     /locus_tag="O2692_000503"
     CDS             34394..35875
                     /locus_tag="O2692_000503"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCZ4298149.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVD"
     gene            35859..36119
                     /locus_tag="O2692_000504"
     CDS             35859..36119
                     /locus_tag="O2692_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HCZ4298150.1"
                     /translation="MALLINKRFIDEGKTIDVYLFEALNNQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            36119..37681
                     /locus_tag="O2692_000505"
     CDS             36119..37681
                     /locus_tag="O2692_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCZ4298151.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     gene            37983..38786
                     /locus_tag="O2692_000506"
     CDS             37983..38786
                     /locus_tag="O2692_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCZ4298152.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            39005..41329
                     /locus_tag="O2692_000507"
     CDS             39005..41329
                     /locus_tag="O2692_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCZ4298153.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTVKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHIPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            41346..42704
                     /locus_tag="O2692_000508"
     CDS             41346..42704
                     /locus_tag="O2692_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCZ4298154.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMILMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            42843..44354
                     /locus_tag="O2692_000509"
     CDS             42843..44354
                     /locus_tag="O2692_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCZ4298155.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFIVKNR"
     gene            complement(44534..44622)
                     /locus_tag="O2692_000510"
     tRNA            complement(44534..44622)
                     /locus_tag="O2692_000510"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44585..44587),aa:Ser,seq:gct)
BASE COUNT        15841 a         6179 c         8093 g        14509 t
ORIGIN      
        1 atgaaattaa tgccaggttc gccatttaac gatgctaaat taaatgctga acaaaaagaa
       61 attttaaatg aaaaatatgg attaaatgat cctgtagcta cgcagtattt acattattta
      121 aaaaatgttg ttacaggcga ttttggtaat tcattccagt atcataatca acctgtgtgg
      181 gatttgatta aaccgagact actaccttct tttgaaatgg gtcttacagc aatgttcatc
      241 ggtgtgatac tgggacttat tttaggtgtt gcagcagcta ctaaacaaaa ttcttgggtt
      301 gactatacaa ctacagttat ttcagttatt gcagtatctg taccatcttt tgtacttgct
      361 gtacttttac aatatgtatt tgcagttaaa ttaagatggt tcccagtagc tggatgggaa
      421 ggtttttcga ccgcggtatt accgtcactt gcattatctg cagctgtttt agcaactgtc
      481 gccagataca taagagcaga gatgatagag gtattaagtt cagactatat tttattagcg
      541 agagctaaag gtaattcgac aatgcgtgta ctttttggac atgcacttag aaatgcttta
      601 attccaatta ttacaattat cgttcccatg ttagcaagta ttttaacagg cactttaaca
      661 attgaaaata tttttggagt tcctggatta ggggatcaat tcgtacgttc aattacaaca
      721 aatgatttct cagtaatcat ggcaatcaca ctattattta gcacactgtt tatcgtttct
      781 atttttattg tagatatttt gtacggtgtg atagatccac gaattcgtgt tcaaggaggt
      841 aaaaaataat ggctgaaaat aaaaacaatt tgtcgattaa cgacgatcat tctaatgcag
      901 ctatgacgca tacctctgac gctatcgcat catctgattt tattattaga gaattagatt
      961 tgaatcagga acctgaaatg caacgagaaa gcaaaaactt ttggcaagat gcttgggctc
     1021 agttaaaacg aaataagtta gctgttgtcg gtatgatagg tttaattatc attgtaatat
     1081 ttgcttttat cggtccagtt ataaataaac atgattatgc tgaacaaaat gtagaacata
     1141 gaaatcttcc ggcaaaaata cctgtattag acaaagttcc atttttacct tttgatggta
     1201 aagatgcaga tggcaaggat gcttataaag cagcaaatgc taaagaaaat tattggtttg
     1261 gtactgatca gttgggtcga gatttatgga caagaacatg gaaaggtgct caaatttcat
     1321 tgtttatcgg tgttgttgca gcgatgttag atatttttat tggtgttgta tatggtgcga
     1381 tttctggatt cttcggtgga cgtgtcgata cgattatgca acgtatactt gaagtcatag
     1441 catctattcc gaatttaatt gtcgtaattt tatttgtatt aatttttgaa ccatccattt
     1501 ggacaattat attggctatg tctatcacag gctggttagg catgagcaga gttgtacgtg
     1561 gagaattttt aaaattaaaa aatcaagagt ttgtcatggc ttcgaaaaca ttgggggctt
     1621 caaaattcaa attgatattt aagcatattt tacctaatac attaggtgct atcgtggtta
     1681 catcaatgtt tacagtacct agtgctattt tcttcgaagc atttttaagt ttcattggta
     1741 taggtgtacc cgcacctcaa acatcgttag ggtcattagt aaatgatggg cgcgcaatgt
     1801 tattaattta tccacatgaa ttatttatac cagcaatgat tttaagttta ttaattctat
     1861 tcttttactt atttagtgat ggattacgtg atgcatttga tccgaaaatg cgtaaataaa
     1921 aagggggcat agcatatgac tgaaagaata ttagaagtaa atgatttgca tgtttccttt
     1981 gatattacag caggggaagt gcaggcagtg agaggcgtag atttttattt gaacaaaggg
     2041 gaaacattgg caattgttgg tgaatcaggt tcaggtaaat ctgtaacaac aaaagcaatt
     2101 acaaaattat tccaagggga cacaggaaga attaaaaagg gagaaatttt atttttaggg
     2161 gaagatttag caaaaaaacc tgaaaatgag ttgattaaat tacgtggcaa agatatttca
     2221 atgatctttc aagatccaat gacatcttta aacccaacga tgcaaattgg taaacaagtc
     2281 atggaaccat taattaagca caaaaattat agtaaagcac aagctaaaaa gcgcgcattg
     2341 gaaatactaa atcttgtagg tttaccaaat gcagaaaaaa gatttaaagc atatcctcat
     2401 caattttcag gtggacaaag gcaaagaatt gttattgcaa ccgcattagc ttgtgaacct
     2461 aaagtgctca ttgctgatga accaacgact gcattagacg taacgatgca ggcacaaatt
     2521 ttagatttaa tgaaagaact acaacaaaaa atcgatacag caattatttt tataacgcat
     2581 gatttagggg ttgttgcgaa tattgctgat agagtggcag ttatgtatgg tggtcaaatg
     2641 gttgaaacag gagatgttaa cgaaatattt tatgatccaa agcatccata tacatgggga
     2701 ttattatcgt caatgcctga tttatcaaca acaaatgaca caccattact agcgattcct
     2761 ggagcgccac ctgatttatt acacccacct aaaggtgatg catttgcgag acgtagtcaa
     2821 tatgcattag atattgattt taaagtagaa ccaccgtggt ttaaagtttc accgacacat
     2881 tttgtgaaat cttggttatt agacgcacgt gcaccaaaag ttgaactacc cgagctggta
     2941 aaacaacgta tgaaaccgat gcctaataat tatgaaaaac cactcaaggt agaaagggtg
     3001 tcgttcaatg aaaaatgatg aagtgctatt atctattaaa aatttaaagc aatattttaa
     3061 cgcaggaaag aaaaacgaag tgagagcgat tgaaaatatt tcgtttgata tatacaaagg
     3121 ggaaacatta ggtttagtag gagaatcggg gtgtggtaaa tctacaactg gtaaatcaat
     3181 tattaaactt aatgatatta caagtggaga aattttgtat gagggtattg atatacaaaa
     3241 gattcgtaaa cgtaaagatt tgcttaaatt taataaaaag atacagatga tttttcaaga
     3301 cccatatgcg tctttaaatc ctaggttaaa agtaatggat atagtagctg aaggtattga
     3361 tatccatcat ttagcaactg ataagcgtga ccgaaaaaaa cgtgtctatg atttacttga
     3421 aactgttgga ttaagtaaag aacatgccaa tcgctatcct catgaatttt caggtggaca
     3481 acgccaacgt attggaattg cccgtgcatt agccgttgaa ccagaattca ttatcgcgga
     3541 cgagccaata tcggcattgg atgtttcaat ccaagctcaa gtagttaatt tattattaaa
     3601 attacaacgt gaaagaggga ttacgttcct atttatagct catgatctat caatggtgaa
     3661 gtatatttca gatcgtattg cagtcatgca ttttgggaaa atagttgaaa ttggaccggc
     3721 agaagaaatt tatcaaaatc cattacacga ttatactaag tctttattat cagccattcc
     3781 acaacctgat cctgaatcag aacgcagtcg caaacgattt agttatattg atgatgaagc
     3841 aaataatcat ttaagacaat tacatgaaat tagaccgaat cactttgtct ttagtactga
     3901 agaagaagcg gcacaactac gagaaaataa attggtgaca caaaattaag gggaaggggg
     3961 aaatgcaatg acgagaaaat ttagaacact tattttaatt ttgattgcta caattgcatt
     4021 aagtggttgt gctaatgacg atggtattta ttcagataaa ggtcaagtat tcagaaaaat
     4081 tttgtcatca gacttaacat cccttgatac atcattaata acggatgaaa tatcttctga
     4141 agtgactgcg caaacattcg aaggtttata cacattagga aaaggtgaca aaccggtgtt
     4201 aggtgttgcg aaagcttttc ctgaaaagag taaagatggt aaaactttaa aggttaaatt
     4261 aagaagcgat gctaaatgga gcaatggtga caaagtgact gcacaagact ttgtttatgc
     4321 ttggagaaaa acagttgacc ctaaaacagg ttctgaattt gcatacatta tgggggacat
     4381 taaaaatgcg agtgatatta gtactggtaa gaaacctgta gagcaattag gtatcaaagc
     4441 attaaatgat gaaacattac aaattgaatt agaaaagccg gttccatata ttaatcaatt
     4501 attagcactt aatacctttg cacctcaaaa tgaaaaagtt gccaaaaaat atggtaaaaa
     4561 ttacggtacg gcagctgata gagcggtata caatggtcca tttaaagttg atgattggaa
     4621 acaagaagat aaaaccttac tatctaaaaa tcagtattat tgggataaaa agaatgtaaa
     4681 attagataaa gtgaattata aagttattaa agacttacaa gccggtgcat cattgtatga
     4741 tactgaatca gtagatgacg cagttattac tgcagatcaa gtaaataaat ataaagacaa
     4801 caaaggatta aactttgtgt taacgactgg gacatttttt gtaaaaatga atgaaaaaca
     4861 atatcctgat tttaaaaaca aaaatttaag attggctatc gcacaagcaa tagataaaaa
     4921 aggatacgtt gattcagtga aaaacaatgg ctcaattcct tccgatacac taacagccaa
     4981 aggaattgcg aaagcgccta atggcaaaga ttatgcgagt accatgaatt cgcctttaaa
     5041 atataatcct aaagaagcaa gagcgcactg ggacaaagct aaaaaagagt taggtaaaaa
     5101 tgaagtgaca ttttcaatga acacagaaga tacaccagat gcaaaaatat ctgctgaata
     5161 tatcaaatcg caagttgaga aaaatttacc aggagttact ttgaaaatta agcaattacc
     5221 gtttaaacaa agagtatcac tagaactgag taacaatttt gaagcatcac ttagtggttg
     5281 gtctgcagat taccctgatc ctatggctta tttagaaaca atgaccacag gtagcgcaca
     5341 aaataataca gactggggta ataaagaata tgatcaatta cttaaagtag caagaaccaa
     5401 attggcactt caaccgaacg aacgatatga aaacttgaaa aaagcagaag aaatgttcct
     5461 aggagatgca ccggtagcac caatttatca aaaaggtgtt gcacatttaa caaatcctca
     5521 agtaaaagga ttaatttacc ataaatttgg tccaaataac tcacttaaac atgtatatat
     5581 tgataaatcg atagataaag aaacaggtaa gaagaaaaaa taatatgctt tgtaaattag
     5641 gctggagaca tatctccagt ctttttgtgt tggataaaaa ctttgggaat aaaaatttaa
     5701 aataagtcgt tttttaaatt actgaaattg attaaatgca taaataactg aatattctaa
     5761 aaataaactt gtaataattt tttctatgag taaactaaaa agaaaaaatt agattgaaag
     5821 taggaggcat atgtatgggg aagctaatta aatatatttc aatacttctt attgtcgttt
     5881 tagtgttgag tgcttgcgga aaaagcagta ataaagatga aggagtaaaa gatgctacta
     5941 aaacggaaac ctcaaaacat aaaggtggta ccttaaatgt agcattaaca gcaccgccaa
     6001 gtggtgttta ttcttcgtta ttaaatagta cacatgcaga ttctgtagtt gagggatatt
     6061 ttaacgaaag cttattagca actgataaaa aaatacgtcc taaggcatat attgcttcat
     6121 ggaaggacat cgagccggct aagaaaatag aatttaaaat taaaaaaggt attaaatggc
     6181 atgatggtaa tgaattgaaa attgatgatt ggatttattc aattgaagtc ttagctaaca
     6241 aggactacga aggtgcttat tatccaagtg tagaaaatat ccaaggtgcg aaagattatc
     6301 atgaaggaaa aactgatcat attagcggat tgaagaaaat agatgactac actatgcagg
     6361 ttacatttga taaaaaacaa gaaaattact taacaggatt tattactgga cctttattaa
     6421 gtaaaaaata tttatcagat gtaccaatta aagatttagc gaaatcagat aaaatccgaa
     6481 aatatcctat tggtattgga ccgtataaag ttaagaaaat cgttccaggt gaggctgttc
     6541 aactcgttaa atttgatgat tattggcaag gtaagcctgc actagacaaa atcaatttaa
     6601 aagttattga tcaagcgcaa attattaagg caatggaaaa aggcgatatt gatgttgcga
     6661 atgatgctac cggtgcaatg gcaaaagatg ctaagtcatc taatgctggt ctcaaggtat
     6721 tatctgcgcc aagcttagac tacggtttaa taggattcgt atctcatgat tacgataaaa
     6781 aagctaataa aactggtaaa gtgagaccaa aatatgaaga caaagaatta cgtaaagcaa
     6841 tgctttatgc aattgataga gaaaaatgga tcaaagcgtt tttcaatggt tacgctagtg
     6901 aaatcaatag ttttgtacca tctatgcatt ggatagcagc caatcctaag gacctaaatg
     6961 attacaaata tgatcctgaa aaagctaaaa aaatcttaga taagttaggt tataaagata
     7021 gagatggtga cggatttaga gaagatccta aaggtaataa atttgagatt aactttaaac
     7081 attattcagg ttctaatcct acttttgaac caagaactgc tgcgataaaa gatttctggg
     7141 aaaaagttgg cttgaaaaca aatgtgaagt tagtagaatt cggtaaatat aatgaagact
     7201 tagcaaatgc atctaaagat atggaagtgt acttcagatc atgggcagga ggtacagatc
     7261 cagatccatc agatttatac cacactgata gacctcaaaa tgaaatgaga acagttttac
     7321 caaaatcaga tcaatattta gatgatgcat tagacttcga aaaagtaggc attgatgaaa
     7381 agaaacgtaa agatatttat gttaaatggc aaaaatatat gaatgatgag ttacctggat
     7441 taccaatgtt ccaaggtaaa tcgataacta ttgttaacga taaagtacga aacttagaca
     7501 ttgaaattgg aactgatcaa agtttatata atttaactaa agaagcttag taatgtaacc
     7561 tcgacgattt tgtcgaggtt tacttatatg gggggatgtt atgaataatg tattgttaga
     7621 ggttaaagat ttagaaacat cattaaaaat aaataatgaa tggttagcaa ctgttgaaaa
     7681 tatttctttt gaattatcta aaggagaagt tttgggtata gtaggggaat ctggttgcgg
     7741 taagtccata ttaagtaagt caattattaa attattacca gaaaagatat ctaaactaag
     7801 taatggagaa gttatatttg atggtaaacg aatcgatacg ctcaatgaga agcaattgcc
     7861 agatattcga ggaaatgata ttgctatgat ttttcaagaa cctatgactg ctttaaatcc
     7921 tgtatttacc ataaaaaatc aacttgtgga atctataaaa tcacataaaa aaatttctaa
     7981 aaaagaagca aataaattag caaaagattt actaaaaaaa gttggaattg ctagacaaga
     8041 tgaaatatta aatagctatc ctcatcaatt atctggtggt atgagacaaa gagtaatgat
     8101 tgcaatggcc atttcatgtt ctcctaaatt attaattgct gatgaaccta caacagcatt
     8161 ggatgtcacg attcaagcgc aaatattaga cttattaaaa gaattgcaaa aggaaacgca
     8221 aatggcaatt atgatgatta cacatgattt gagtgtagtt gctgagtttt gcgataaagt
     8281 cttagttatg tatgcaggtc aaattgtaga atttggaggc ataaaagaaa tactacacaa
     8341 tccgaaacat ccttataccc aaaaattatt atcaacaatt ccaaaactta aagaagagca
     8401 gaaacgactt gaaacgatag aaggaattgt gccatcaatc caagcatttc acgttaataa
     8461 gtgcagattt gcaaatagat gtaacaaaaa actggatatt tgtaataatc aatctcctaa
     8521 aatgcatgtt tgtgaagatg tcattgtacg ttgtcatttg tacaaaaatg aatataagga
     8581 gatataagaa tggaaaatat tttagaagtc aaccaaataa aaaaatacta caaaattaaa
     8641 actggattat tacaaaaaac tcagtacgtt aaagctgttg atgacgtatc gttttcaata
     8701 aaaaaaggac aaacttttgg attagtagga gaatcgggtt gtggtaagtc aacgttaggt
     8761 aaagtgatta tcaggcttga agatgcaact tcaggctcaa taattgttaa tggtgaagat
     8821 ataacaagat tacaaggtaa aaaactcaga aaatcacgac aacaatatca gatgatattt
     8881 caagatccgt atgcatcatt gaatccgatg caaatggttg gagatatcat ttcagaacct
     8941 attttaaatt ataaaaaatt gccaaaagaa gaaataaaaa aagaagtact atatttatta
     9001 aaatgtgttg gcctaagtga agatgcatat tataaatatg cacatgaatt ttcaggtgga
     9061 cagagacaaa gagtgggaat tgcaagagca ttggctttgc gtccgagttt aattgttgct
     9121 gatgagcctg taagtgcatt agatgtatct gttcaatctc aagtactgaa tttattaaaa
     9181 gatttacaag aacaatttaa cttaagctat ttatttatcg cacatgattt aagtgtagta
     9241 aaacatataa gtgatgtcat tggagttatg tatttaggtc atatagttga aatcgcatct
     9301 gataaagaaa tttatgaaaa tcccaaacat ccatatacaa aagcgttgat ttcatcaata
     9361 ccacaaattg ataaacataa taacaataga attatattaa aaggagaatt accttcgcca
     9421 agtaatccgc cgcaaggttg tccttttcat acaagatgtc caattgctca agatatgtgc
     9481 aaaaaaagta tgccagaatt aaaggacatt ggtaatgaac atcaagttgc ttgtttctac
     9541 gtagataaag taggtgattt aaatgattaa attaatactt aaaagattag ggttaatgat
     9601 cccgttacta attttaatct caatagttgt attttcatta gcaatcatac aaccaggaga
     9661 tccattttca gatctacaaa acggtaaaat aaaacaagaa gcaataaatg cacaaaggga
     9721 aaaattaggt ctcaacgact caataccaca tcaatacatt agatgggtca atcatgttat
     9781 acacggtgat ttaggtgagt caatcaaata taaaaggccg gtaattgatg ttattgagga
     9841 aagaattcca aatacaatat tactcggtgc tatgtcatta attattactt atattatctc
     9901 atttgcttta ggaataacgt caggtagata ttcttacagt ttgacggatt atactgtgca
     9961 aatatttaat tatttgatgt tagccattcc atcttttatt gcgggagtat ttgcaatttt
    10021 tattttttct tttgaattac aatggttccc gtttcaaggt tctgttgata ttaaccttaa
    10081 agaaggtact tttgaatatt atatgagtaa aatatatcac acatttttac ctgcattcac
    10141 tttaggttta ttatctactg ctggttatat tcaatattta cgtaatgata ttattgaaaa
    10201 ttctaaaaaa gattatgtat tgacggcaag gtcaaaagga ttatctatga ataaaattta
    10261 taataaacat atattgagaa attctctaat acctattatt acatttttag gtgctgatat
    10321 tgtaagtatt ttaggtggag ccgtgattac ggagactatc ttttcatata acggtatcgg
    10381 taaattattt ttagaatcgg taataggcca agactatcca ttaatgatgg cattaacgtt
    10441 atttttctca tttttaggtt tactgggtaa tttgatttct gatattactt atggatttat
    10501 agatccaaga attagaagta actagggggg attagaatgc aaaataagtc aaaatcgcct
    10561 tttaaaattg cattttctaa atttattcat aataaaattg caatgttatc ggttattttt
    10621 ttattaatca taactattgt atcaattata gcgccattaa tagctccttt tccagtgaac
    10681 caacaagatt tattaaatat aaaaggtgaa atgacagcac aaaacattct tggtacagac
    10741 tctggtggta gggataactt tagtcgtttg ttatatgcag gtcgtatttc attatccatt
    10801 ggaattacat ctacaatagg aatgcttttg attggaatta cagttggagt gatttctggt
    10861 tattttggag gtattgttga tacattatta atgagaataa ccgaatttgt tatgttattt
    10921 ccatttttaa tatttgcaat tgtattaaat gctgcacttg gagataaaat taaaaatcct
    10981 tatggatctg ccataattct tgttctagtt attatcgtat taagttgggg aggtattgca
    11041 agacttgttc gtggtaaagt acttcaagaa aaagaaaatg aatacttttt ggcagcaaaa
    11101 tcaattggta cacccacata taaaattatt ttgaaacatc ttttgccgaa tatattaagt
    11161 gtagttatcg tacaagcaac attgttattt gccggtatga ttgtagtgga atcaggattg
    11221 agctttttag gattcggaat tagtaaagca ataccatctt ggggtaatat gttgagtgat
    11281 gctcaagaag gggatgttat aagtggtaaa ccgtggatat ggatgccacc tgctataatg
    11341 attacattaa ctatattaag tataaacttt gtaggggaag ggattaaaga tgcttttaat
    11401 cctagaggta gacgttaaat aataaaagag gcactagtta attctagtac ctctttattt
    11461 tatctcttac gtcctaaacc catcgctttt tccatttttt tgacagtttt aaatgaaact
    11521 ttgtgtgctt tatctctacc ttgatctaaa atatcatcaa gtttatctga gttatagaaa
    11581 ctttcgtatt tttcttggaa ttctactaaa aatgctttaa ctatttcagc aaggtcacct
    11641 ttaaatttac cataaccttc gccctcatat tttgcctcaa tatctttaat tggcatgtct
    11701 gttaatccag cgtatattga aattaaattt gttatacctg gcttgttgtc acgatcaaat
    11761 ttaataatac catctgaatc agttactgcg cttttaattt ttttagctgc aacattcggc
    11821 tcgtctaata atgaaatgaa gtttttagca ttatcatcac tcttactcat ttttcttgtt
    11881 gggtcttgta aactcatgac acgtccacca actttaggca tacgaatttc aggtttcaca
    11941 agcacatcat tatagcgact attaaatcta tctacaaggt tacgagtcaa ttcgatatgc
    12001 tgcttttggt catctccaac tggaacgata ttagtattgt aaagaacaat atcagctgcc
    12061 attaaaggtg gatatgttaa tagaccagca ggtatacctt caactgcttt ctgagcttta
    12121 tctttgtatt gcgtcatacg ctctaattct ccaacagaag caatcgtagt taacatccat
    12181 cctgcttgta cgtgtgcagg gacttcagat tgtatgaaca atgttgcttt gtctggatct
    12241 ataccagaag ctaaataaat cgctgctaat tgtctggtct gtttacgtaa ttttaaacga
    12301 tcttgtggca ttgtaattgc atgttgatct acgatacaga aataacaatc atagtcattt
    12361 tgcacatcaa caaattgttt tagtgcgcca atataatttc caatagtagg aattccacta
    12421 ggttggatgc ctgaaaataa tgtctccatt taaatgccta ctttcttagt aatattttaa
    12481 taattatcat tataacagta tttcttaaaa atgtaagatg tgttagaata tacgtataac
    12541 ttactactaa caaagggatt aataatcttt tttataagtg tagaaactta tctgaaaaat
    12601 taggtttttc tcatttaatt ttaatgttag atagttataa taaatagtaa cgattaagaa
    12661 ttttagaaaa aaatgttttt tcttgatcta taactaaatt ttaagcaatg aattttattc
    12721 attcaaatag gagagtgaga tgtatggtaa cattatttac ttcaccaagt tgcacatctt
    12781 gccgtaaagc gaaagcatgg ttacaagaac atgacattcc gtatacggag cgtaatattt
    12841 tttctgaaca tttaacaatt gatgaaatta agcaaatatt aaaaatgact gaagacggta
    12901 ctgatgaaat catttctaca cgttctaaaa cataccaaaa attaaatgtt gatattgatt
    12961 cactaccatt acaagactta tattcaatca ttcaagataa tcctggctta ttacgtcgtc
    13021 caattatttt agataataaa cgactacaag ttggttataa tgaggacgag attcgacgtt
    13081 tcttacctag aaaagttcgt acgttccaat tacaagaagc acaacgtatg gttgactaat
    13141 aaccatttaa ctatgtgtca tactttacta aaagatatgt caagcaacac tatttctata
    13201 gtgttgcttt tttggtttta attttacaat aattgcttag tatcttttaa atcaaatgtg
    13261 caattgttaa tatagctcta tattagagtg ttttggatga aacatttgct atgataaggt
    13321 aaaattaaat taagaaagtg ctttaatgct ttaactgtat aaatcattta aatagttttg
    13381 ctacaacgaa tatgttatta taagttacca aaatatttta gagaaagaaa tcaaacaaat
    13441 aaattgtatt ttgtttgatg ctttaataca atgtggatac tattcatcga ctgtaaggag
    13501 tgagatgata tgagaataga acgagtagat gatacaactg taaaattgtt tataacatat
    13561 agcgatatcg aggcccgtgg atttagtcgt gaagatttat ggacaaatcg caaacgtggc
    13621 gaagaattct tttggtcaat gatggatgaa attaacgaag aagaagattt tgttgtagaa
    13681 ggtccattat ggattcaagt acatgccttt gaaaaaggtg tcgaagtcac aatttctaaa
    13741 tctaaaaatg aagatatgat gaatatgtct gatgatgatg caactgatca atttgatgaa
    13801 caagttcaag aattgttagc tcaaacatta gaaggtgaag atcaattaga agaattattc
    13861 gagcaacgaa caaaagaaaa agaagctcaa ggttctaaac gtcaaaagtc ttcagcacgt
    13921 aaaaatacaa gaacaatcat tgtgaaattt aacgatttag aagatgttat taattatgca
    13981 tatcatagca atccaataac tacagagttt gaagatttgt tatatatggt tgatggtact
    14041 tattattatg ctgtatattt tgatagtcat gttgatcaag aagtcattaa tgatagttac
    14101 agtcaattgc ttgaatttgc ttatccaaca gacagaacag aagtttattt aaatgactat
    14161 gctaaaataa ttatgagtca taacgtaaca gctcaagttc gacgttattt tccagagaca
    14221 actgaataac gatattaatt aagcaacgca ctatgaaagg agactctttg gagtctcttt
    14281 ttttattgat ttcaaaagct gaaaaagtaa tagtagttaa acttacgtgt aatattttga
    14341 ggtgaagata tgttagtagc tttaaatgaa gaaaaggaac gcgtattagc aactactgca
    14401 ttgagaaaga cacaatattt ttgtccggtg tgtggcaagc aagttatttt aaagcgtggg
    14461 ctcaaagtaa ttagtcattt tgcacataaa catttagcgg aacaaaaatg ttttaataat
    14521 gaaacgatta aacattataa aagtaaattg attttagcac agatgataca gcaacaagga
    14581 tgtaaagtag agatagagcc attttaaaag aaataaaaca aattccggat attttgatta
    14641 ataataaata tgttattgag ctacagtatt cgccaattcc ttataaacag attcttcaac
    14701 gaacggaagg tttaaagaaa atgggatata aagtaagttg gttattaaat gatgttgatt
    14761 attgtcataa taaagtgaag ttcaatcatt ttcaaagtat gtttattaat ccattcactc
    14821 gaaaacttca tacgttcaat ttagagaaaa aacaaataat gatgtttcaa caaatacaat
    14881 atttaggcgg gcacaaatat gtcgctgaaa aaagaaatgc caaaattagt gagttgttta
    14941 atgaggcgcc ttgtgattat catgctgttt ataaattatc aaagttcgca attaatcaat
    15001 atatcaaata ttgtcgctgg caaaattctg ttttagaacc cactttaagt gcaatgtatc
    15061 aattacagtt aactgatcaa gaagtagtgc acaattatgg ttatattttt ccagagcaaa
    15121 tttatattaa aaatcatcct attgagtggc aattacaagt tgatttatgg ttaaagaatg
    15181 gaaaaagcaa attagtaaat gacaatctta attattttaa actgaaaaaa tttattgttg
    15241 ctctagaaag taaaacagca attatagaaa aacttattaa caattattta aatatttgtt
    15301 cagataaagg taatgacgtg caaattttgt tctaaaatga gaaaattata tcaattatat
    15361 tttattagga ggtttactac atatgagtca acaattatcg agagaagaac aggaacgtaa
    15421 atatcctgaa tatacatggg acttaacaac aattttcaaa gatgatgaag cttttgaggc
    15481 tgcatttaaa gaagttgaaa atgagttagg caaagaagaa caatttaaag gacacattgg
    15541 tgatagtgct gagacattat acaatgcgtt agaattagaa gatacattag gtactaaatt
    15601 agaaaaagta tatgtatacg cgcacctaaa acaagaccaa gatacaacga acgacaagta
    15661 tactggtatg gagtcaagag cacatcaatt aattattaaa tttagctcgg catggagttt
    15721 cttagtgcca gagattttac aaattgatga agataaaatt caatcatttg taaattcata
    15781 tgataaatta caaaaattcg catttgattt gaagttgatt aatgaaaaac gtcctcatat
    15841 tttagatgct gaaactgaaa agttattaac agaagcgcag gacgcgttat caacgccatc
    15901 aaatgtatac ggtatgttta gcaacgctga tttagtattt gaagatgcga tagataaaga
    15961 tggaaatgca cacccgttaa cacaaggtac atttattaag tatttagaaa cagatgatcg
    16021 caaactaaga gaaagtgctt ttagaaatgt atataaagca tatggtgctc ataataatac
    16081 gcttggcgct acgctagcag gtgaagtgaa gaaaaatgta tttaatgctc gtacacacaa
    16141 ttacaaaact gcaagagaaa aagcattgag taataatcat attccagaaa atgtatatga
    16201 caatctagta aaaactgtac ataaatattt accattgcta catagatata ctgaattgcg
    16261 caaagaattg ctaggtttag atgacttgaa aatgtatgat ttatatacac cattaattaa
    16321 agatattaag tttgaaatgc cttatgaaga agctaaagag tggatgttaa aagcattaga
    16381 accaatgggt gaagaatatt taaatgtagt taaagaaggc ttaaacaatc gttgggtcga
    16441 tgtctatgag aataaaggta aacgttcagg tggctattca tcaggtgcac atttaactaa
    16501 tccatttatt ctacttaact ggtctaatac tatttcagac ttatacacat tagttcatga
    16561 atttgggcat tcagcacata gttacttcag tagaaaattc caaccgtcaa attctagtga
    16621 ctacactatt tttgtcgctg aagttgcatc aacttgtaac gaagcacttt taagtgatta
    16681 tatggataaa catcttgatg atgaaaaacg cttattatta ttaaaccaag aattagaacg
    16741 tttcagagct acattattcc gacaaacaat gttcgcagaa tttgagcata aaattcatgc
    16801 aattgaagaa gcaggtgaac cattaacgcc aactagaatg aatgaagaat atgccaaatt
    16861 aaataaatta tacttcggtg attctgtaga aactgatgaa gatattagta aggaatggtc
    16921 acgtattcca cacttctata tgaattatta tgtatatcaa tacgcaactg gttacagtgc
    16981 agctcaaagc ttaagtcatc aaattttaac agaaggtaag ccagcagtag atagatatat
    17041 taatgaattc ttgaaaaaag gtagctcaaa ttatccaatt gagatattaa aaaatgctgg
    17101 tgtagatatg acaacacctg aaccaattga acaagcttgt gaagtttttg aacaaaaatt
    17161 gaacgctttt gaaaaattaa tgaaagctta gtactattgt attcgcttac attatgaaca
    17221 gattgtgaca atttaaatta agtactatta aaaggttgaa atggtagaga tagcatgtta
    17281 tattatgaac atgaaattaa tcacataaca aacatacccc tttgtttgaa gtgaaaaatt
    17341 tctcccatcc cctttgttta gcgtcgtgta ttcagacacg acgttttttt atgttctttt
    17401 ttaataagag ggaagtgaag ttgtataaaa atgtattaaa tatgcacttt ttatacatta
    17461 acgattcata tatgtctatt atgtaccaaa tttataattt gtataatttt cgaactggtt
    17521 aaattcgaaa aatatcacta atatatttag atcgatgtgt aacattacgt tctaataatt
    17581 taatgttgca aaattaagag tcagtaaaaa gacctcgtta catttatggt gtacgaggtc
    17641 tttaatttat ttattttgat ttgattttag gcattttaga tttccagaaa tcgccatctg
    17701 gatatttaag tttttcaatt ttttgttgaa tggctaactt ctttaactct ttgtttaaaa
    17761 gtttttctgg ccattcataa atagtaagta attcttccat cgttacaagt tgttgttgct
    17821 gtatataagt ttctaattta ggaggaagat tcttttcgat aggtttaccc atcaattcat
    17881 taattatata agtatagatg tgatatgggt ataatccttc gacttttaaa ccttcttcat
    17941 gaacatcttc actaaagaaa acgagagaag gggcttgttc gatttccatt tctcttgcaa
    18001 tatgcaaatc aattttcaag ctttcggtta gtttactttt ttgtaagtcg tctttaaata
    18061 cttctaaatc aatacctgca ttttgaatac agtcacaaat cattgattct gtaataatat
    18121 ctcttttagg tatgatttca ttttgcatta aatgtataaa tcgttcggca cgtacacgac
    18181 cttgtaactc agctgcttta taagctaggg cgatgttatc aaagttggat gtactttgag
    18241 cttggcattt cgttaatact tttaacgaag gatttaatat atgtctgata cgtatatatt
    18301 gattatattc aattcttaat ttggataaga ttgctgataa tttgaagcaa tcggagctaa
    18361 atggatcgaa aaatgaataa atttcgattt tacttacagg tgatagattg atatcttcac
    18421 gactcttatt ttccattatt cgtaattctc cagccatgtt tattcaccta caattaattt
    18481 taggaattca ccatatgatt agcagttaat cttaagcgct caaataaata atctccaaca
    18541 ccttgtggaa acgcagcgcg attaattgct gtctgcatat tttctaacca tgcatctctt
    18601 tcaaattcag tgattgtaaa atctatatgt ctttttctta gcataggatg tccgtgttct
    18661 tcggtataaa tgtttggacc gcccaaaaac tgtgttaaaa attgtttttg tttacgactt
    18721 gtttctgcaa aatctcctgg aaacaggtga ttaagtcgtt catctttttc tacaagggtg
    18781 taaaaataat gaatcatatc gtataacgct tctttaccaa tgatgtcata tggtgttgtt
    18841 gtcatttaat cacccatttt caaaaattta ctgttacgaa cttaagttaa tatataacta
    18901 atataacatg attttaaaca tttgaaagaa atatgcatat ttgccaattt aatttatatt
    18961 gtttgaaagt gtttcttttt tcttgaaaaa acgttgaact ttatttaaag gttgatgatg
    19021 ttcgaggttt agttcgttta ataaagattg gaacttttgt aaaccttgat tatagtcttt
    19081 aacttcgaac tctaactcat aatccgtagt atcgaaatac tcacttttat ctaaaaccag
    19141 taaatcacct ttatatttag tttcttggcg atatgtcgtt aatgcaccaa gtattgataa
    19201 agttgtatct tttacaccaa actgttcaac tataatttga cgaatgtcat ctggaagatt
    19261 gtcgtttgaa ataatcaagt tcatctctgg tttaatgtcg acgatatagt tgtattctaa
    19321 tagaccaacc tttgctggtg tttttaaagt catttcatat tgattgtctt taactcttat
    19381 gcgtagtgca gagcgatgtt cctttaattt gaaatcgggt gtatcaatat agtaattgac
    19441 ttgcttaaaa agcacactgt ctttaaaata tttctcttgc aatttattat agattgatgc
    19501 agttatcatt tgtttaaatt ctatttcatg atttgttgcc atgatatgta tacacctcgt
    19561 atcaaattca atttatctta actatattat gaatgacaaa gttgaatttt aaaagtaatt
    19621 tcctttatct attatcaatg ttaatttgac cattaaaaat agtgttcgta agtgttttgt
    19681 attattgaat tgtgttaaaa tgttatggaa taagaggagg attaagcatg cgtttatata
    19741 ttaatgaaat taaaattaaa gatgacatac tttattgtta tacagaagat tctattaaag
    19801 gattatctga agtaggacaa atgctcgttg atagtgataa ttatgccttt gcgtatacat
    19861 tagatgatgg taaagcgtat gcttatctca ttttcgtaca agaaacatgg acgatgttgc
    19921 atgaaaacat gactaaaaaa attattatca atgatgaact agaattgact gaattccacc
    19981 aagaacttac ttatatttta gacaacataa aagggaataa taattatggt aaggaatttg
    20041 ttgcaaccgt tgaagaaaca ttcgacattg aataaagcgg ggtgaagcac tatgaatcaa
    20101 tgggatcagt tcttaacacc ttataagcaa gcggttgatg agttgaaagt gaaacttaaa
    20161 ggcatgcgca aacaatatga agttggtgaa caagcgtcgc caatagaatt tgttactggt
    20221 cgtgttaaac caatcgctag tattatagat aaggcaaaca aacgacaaat accatttgat
    20281 aggttaagag aagaaatgta cgatatcgct ggtttaagaa tgatgtgcca atttgttgaa
    20341 gatattgatg ttgtcgtcaa tattttaaga caaagaaaag attttaaagt aattgaagaa
    20401 cgagattata ttcgtaacac taaagaaagt ggttaccgct cgtatcatgt cattattgaa
    20461 tatccaattg aaacattaca aggccaaaaa tttatattgg ctgagattca gattcgtaca
    20521 ttagcaatga atttctgggc aacgattgaa catactttac gatataaata tgatggtgct
    20581 tatccggatg aaattcaaca tcgtttggaa agagcggcag aagcagcgta tttacttgat
    20641 gaagagatgt ctgaaattaa agatgaaatt caggaagctc aaaaatatta cacgcaaaaa
    20701 cgttctaaaa aacatgaaaa tgattaacga ggtgttataa atcatgcgtt atacaatttt
    20761 aactaaaggt gactccaagt ctaatgcctt aaagcataaa atgatgaact atatgaaaga
    20821 ttttcgcatg attgaggata gtgaaaatcc tgaaattgtt atttcagttg gtggtgacgg
    20881 tacattacta caagcattcc atcagtatag ccacatgtta tcaaaagtgg catttgttgg
    20941 agttcataca ggtcatttag gattttatgc ggattggtta cctcatgaag ttgaaaaatt
    21001 aatcatcgaa attaataatt cagagtttca ggtcattgaa tatccattgc ttgaaattat
    21061 tatgagatac aacgacaacg gctatgaaac aaggtattta gcattaaatg aagcaacgat
    21121 gaaaactgaa aatggctcaa cacttgttgt ggatgttaac ttaagaggga aacactttga
    21181 gcgatttaga ggcgatggat tatgtgtatc aacaccttcg ggttcaacgg cttataacaa
    21241 agcgctaggt ggcgcactga tacatccttc acttgaagca atgcaaatta cagaaattgc
    21301 ctcgataaat aatcgtgtgt ttagaacggt aggatcacca cttgtattac caaagcatca
    21361 tacatgttta atatcaccag ttaatcatga taccattaga atgacgatag atcatgttag
    21421 tatcaaacat aaaaatgtta attcaataca ataccgtgta gcaaatgaaa aagtgaggtt
    21481 tgcacgtttt agaccattcc cattctggaa acgtgtgcac gattctttca tatcaagtaa
    21541 tgaagaacga tgaaatttaa gtatcatata tcacaacaag aaactgttaa aactttttta
    21601 gcacgacatg atttttctaa gaagacagtg agcgccatta aaaataatgg cgctttaatt
    21661 gttaatgatg aaccagtcac agtgcgtaag caattaatga caaatgatat attagaaatt
    21721 catttaccgc gagaaatacc gagtgttaat ttaatacctt atgctcgtaa gctagaagta
    21781 ttgtatgaag atgcttttat catcatagtt actaaaccaa acaatcaaaa ttgtacgcct
    21841 tcgagagaac atcctcatga aagtttaatc gaacaagtac tatatcattg tcaggaacat
    21901 ggtgaaaata ttaacccaca tattgttacg cgtctagatc gtaatacaac tggtattgtg
    21961 atattcgcta aatatggaca tatccatcat ttattttcta aagtaaactt gaaaaaaata
    22021 tatacttgcc ttgtatatgg taaaacccat acatctggta ttattgaagc taatattaga
    22081 cggtcaaagg ataggattat aactagagaa gttgcctcgg atggtaaata cgctaaaaca
    22141 tcttatgaag taataaatca gaatgataaa tacagtttat gcaaagttca tttgcatacg
    22201 ggacgtacac atcaaattcg tgtacatttt caacatattg ggcatccaat tgtgggagat
    22261 tctttgtatg atggttttca tgacaaaatt catggtcaag tactgcaatg tacgcaaata
    22321 tattttgttc atccaatcaa taagaacaat atttatatta caattgatta taagcaatta
    22381 cttaaattat tcaatcaact ctaattcaca cagggggtgt aagtatgtca atgaacacag
    22441 atgaaaaaga gcgtgttcaa gaggaattat acgaccaaac tttattagat caatatttag
    22501 aaaatgatga tattgatcaa tttagagatg aatttctagc attacacaca tatgaacaaa
    22561 gtgagtattt tgaagatact accgatgaaa atagacaaaa gatttttcaa tatttatcac
    22621 ctgaagaagt tgcaaatttc tttgatcaat tagatattga tgacgatgaa tatgagttgc
    22681 tatttgataa gatgaatgcg acatacgcaa gtcacatatt agaagaaatg tcatacgaca
    22741 atgcagtaga tattttaaat gagttgacta aaccaaaagt tgctagtctt ttaacattga
    22801 tgaataaaga tgacgcgaat gaaatcaaag cattacttca ctatgatgag gatacggccg
    22861 gcggtattat gacgacggag tatttatcac ttaaagcgca tacgcctgtt aaagaagcat
    22921 tattattggt caaagcgcaa gcaccagacg cagaaacaat atatgttata tttgtcgttg
    22981 atgatgatgg taaattagta ggtgttttat cgctaagaga tttaattgta gctgaaaatg
    23041 atgcttatat tgaagatatt atgaatgaac gtgtcattag tgtgaatgta gcagacgacc
    23101 aagaagatgt tgctcaagtt atgagagact atgatttcat ggctgtacct gttatagatt
    23161 accaagaaca tttgcttggt atcatcacga ttgatgatat tttagacgtt atggatgaag
    23221 aggctagtga agactactct cgtttagccg gggtatcaga tatcgattcg actaatgatt
    23281 caatcattaa aacagcatta aaacgtttac catggttgat tattttaaca tttttaggaa
    23341 tgattactgc gacaatttta gggagattcg aaaaaacatt agaaaatgta gcgctactcg
    23401 cagcgtttat tcctattatt agtggtatgt caggaaattc aggtacacaa tctttagccg
    23461 tttcagttcg taacattacg acaggggaaa ttaatgagca aagtaaattt agaattgcat
    23521 taagagaagc aggaagtggt gtattatcgg gtgttgtatg ttcaacaata ttatttacaa
    23581 ttattgttgc aatatatcat cagccacttt tagcattaat cgttgcagga agtttaactt
    23641 gtgcgatgac ggtggggacg tttgtaggtt cgatgattcc attattgatg aataaattaa
    23701 atatcgatcc agcagtggct agtggaccat ttattacaac aattaatgat attattagta
    23761 tgttgattta ttttggttta gctacatcat ttatggctta cttaatttaa ggaggagtta
    23821 tggagttttt atctttagtt attgttgttt tagcagcgtt tttaactcca ataattgtca
    23881 atcgattaaa tattaatttc ttgccagttg ttgttgcaga aattttgatg gggattgtga
    23941 ttggaaattc atttctaaat atagtagaaa gggattcaat tctaaatatt ttatcaacgt
    24001 taggctttat ctttttaatg tttttaagtg gtttagaaat tgattttaaa gcttttaaaa
    24061 aagataaacg cgcacgtcaa ggacaaaatg atgatgaatc ctcaattcca gggcatctta
    24121 atctagcgtt aactgtattt gcatttatta tgattatttc gattctttta gcgtatgtat
    24181 ttaaatggct tggattagtg gatgatgtgt tattaatggt cattatcatt tcaactattt
    24241 ccttaggcgt agttgttcca actttaaaag aaatgaatat tatgagaaca actatagggc
    24301 aatttatcct attagtagca gtacttgcgg acttagtaac tatgatttta ttaacggtct
    24361 atggcgcaat caatggtcaa ggcggcagta caatatggtt aataggtata ttagttgttt
    24421 tcacagcaat ttcatatatt ttaggtgttc aatttaaaag aatgtcattt ttacaaaaat
    24481 tgatggatgg tacgacgcaa atcggtattc gtgcggtatt tgcattaata atattattag
    24541 tagccctagc agagggagtt ggcgcagaaa atatattagg tgcattctta gcaggtgtcg
    24601 ttgtttcatt attaaatcca gatgaagaaa tggttgaaaa gttagactca tttggttatg
    24661 ggttctttat tcctattttc tttataatgg ttggtgtaga tttaaacata ccttcattaa
    24721 ttaaagaacc gaaattacta attatcatac cgattttaat cgttgcattt atcatttcaa
    24781 aattaattcc agtcatgttt attcgacgtt ggtttgatat gaaaacaacg attgcatcag
    24841 catttttatt aacatcaaca ttatcgctcg tgatagctgc agccaaaatt tcagaaagat
    24901 taaatgctat ttcagctgaa acgtcaggta tattaatttt aagcgcagtc attacatgtg
    24961 tattcgttcc gattattttc aaaaaactgt ttccagttcc agatgagttt aaccgtaaaa
    25021 ttgaagttag tttaattggt aaaaatcaat taacgattcc tatagcgcaa aatttaacat
    25081 ctcagttata tgacgtgaca ttatattatc gcaaagactt gagtgatcgt cgtcaattgt
    25141 cagatgatat cacgatgata gaaattgctg attatgaaca agatgtttta gaacgactag
    25201 gtctgtttga ccgagacata gttgtttgtg ctacgaatga cgatgatatt aaccgaaaag
    25261 ttgctaaatt agccaaagca catcaagttg agcgtgtcat ttgcagactt gaaagcacaa
    25321 cggacgatac agagttagtt gattcaggta ttgaaatttt cagtagctac ttaagtaata
    25381 aaatcttatt aaaaggttta attgaaacac ctaacatgtt gaatttatta agtaatgttg
    25441 aaacgtcact atatgaaatt caaatgttaa attataaata tgaaaatatt caattacgta
    25501 atttcccatt cggaggagac atcatcttcg tgcgtattat ccgtaataat gagtcgattg
    25561 ttccgcatgg agatacacaa ttgcgatatg gagatcgctt aattgttacc ggtgctaaag
    25621 aatacgttga tgaattgaag caagagttag aattttattt ttaacaataa tgatattaaa
    25681 atgcagacgt attttagtac gacgtaaaat taatgatttt aaaatgctag tatgtatatg
    25741 attttgataa ataaatgctt tttacgtaaa tcaagtttga tacagaaagg actaaatcaa
    25801 acatttatcg ttgtaatacg tttaaataac tttattaaaa agtcataata gtgttaaaat
    25861 gtattgacga ataaaaagtt agttaaaact gggattagat attctatccg ttaaattaat
    25921 tattataagg agttatctta catgttaaat cttgaaaaca aaacatatgt catcatggga
    25981 atcgctaata agcgtagtat tgcttttggt gtcgctaaag ttttagatca attaggtgct
    26041 aaattagtat ttacttaccg taaagaacgt agccgtaaag agcttgaaaa attattagaa
    26101 caattaaatc aaccagaagc gcacttatat caaattgatg ttcaaagcga tgaagaggtt
    26161 attaatggtt ttgagcaaat tggtaaagat gttggcaata ttgatggtgt atatcattca
    26221 atcgcatttg ctaatatgga agacttacgc ggacgctttt ctgaaacttc acgtgaaggc
    26281 ttcttgttag ctcaagacat tagttcttac tcattaacaa ttgtggctca tgaagctaaa
    26341 aaattaatgc cagaaggtgg tagcattgtt gcaacaacat atttaggtgg cgaattcgca
    26401 gttcaaaact ataatgtgat gggtgttgct aaagcgagct tagaagcaaa tgttaaatat
    26461 ttagcattag acttaggtcc agataatatt cgcgttaatg caatttcagc tggtccaatc
    26521 cgtacattaa gtgcaaaagg tgtgggtggt ttcaatacaa ttcttaaaga aatcgaagag
    26581 cgtgcacctt taaaacgtaa tgttgatcaa gtagaagtag gtaaaactgc ggcttactta
    26641 ttaagtgatt tatcaagtgg cgttacaggt gaaaatattc atgtagatag cggattccac
    26701 gcaattaaat aatatcattc aacagctttg ttcacgttat tatatatgtg agcaaagctt
    26761 ttttgctttt ataataatcg gactgatgga aaattatttg atatttcatt tgactgattt
    26821 tttatgcaca aaaaaatcgc ctcaaaagct gtagtttcaa cttttgatgc gatagtatct
    26881 tatattctta aaagacatcc acagaattag tccttaacat tactgtttgc tttatcaata
    26941 atgcgttggc ggtatttgaa aatattacta acaaccgttt taagtacagc atataatggc
    27001 actgcaatca gaactaatgt aaagccacct aaatctcctg ctgctaaaat aacaacgata
    27061 attgttaaag gatggatact taaagattta cccattacat ttggtgtaat gatattacct
    27121 tcaagttgat gtgcaattaa tgtaatgata caaacccata taaatgtagt aggactatct
    27181 ataataccga gtattgctgc aggtgcaaat gataaccatg aacctaagaa aggaattaag
    27241 tttgcgacac cagcaaatag tactaataaa ggaatatatg gtaagtcaat aattgaataa
    27301 ccgatatata agaatatacc taaaataaca ctgacagtta cttgaccttg aatgtaagat
    27361 tttaatgtaa agtttaaatc agttaataaa tctacgaaaa atactttacg ttcacctttg
    27421 aaaaatttag caacagctgg gataaatttt tcatggtctt ttaacatata aattaagaag
    27481 aatggaacca taatcaataa gaagatggtt gaaattaatg atgtaatgta ctgtaatgaa
    27541 ttagataaaa tattagtaac gccatcaccc attgatttaa ccatatttgt aattctactt
    27601 gttacatctt caggtaattt atccatttga gctagtgcga atttaataat ttgctctgct
    27661 tctttttgta gggcaggtgt ctgactaatt aaattgttga tatttgaaat gatgattggt
    27721 gcaacaaacg caacaattaa gccgataata gcaaacaaag ctaacatgat tgttgttata
    27781 ctagcccatc ttggaaaacc gactttttca agtaagtttt gaaaaggtag acagatataa
    27841 aataaaaaac cactaattaa aaatggaaga aatacagaac cgatgattgt agctattgga
    27901 gtaaatactt cgtgcacttc cataaatagt ttgatgagaa tgaacagcat aatcagagcg
    27961 atgccagttc ggaaccaaac cttgtttaac ataaacgatg ttcctcctat ttttctaaac
    28021 ttagttatta tttagtatat cctaaatagc agtgcttaaa aaggttaata ttttttaaat
    28081 atacttaaat taaccattca ctttctattt taagatattt actttcaata gtttgtgaga
    28141 cttttcaatg actcgtaagt ctgcaattgg atcaaacagc accagtttat atgaataact
    28201 tattttaata ttaaacgtgt ttaaatagtg aaatgatcat attaatattt ataagaatat
    28261 tctgaaaaat atattgaagt gtctcgtgct taatcgtata atatttttat gtttaaacaa
    28321 tagggggaat cttatgattg aaaaattagt aaccttttta aatgaggttg tttggagtaa
    28381 gccattagtt tatggtttgc taattactgg tgtgctattt acattgcgta tgcgattttt
    28441 tcaagttaga cattttaaag aaatgattcg attaatgttt caaggagaga agtctcctaa
    28501 tggtatttca agttttcaag cgatagccat gtctttagca ggcagggttg gtacaggtaa
    28561 tattgtcggt gtatctactg caatatttat aggaggacct ggtgcagtat tttggatgtg
    28621 gattactgcg tttttaggtg caagtagtgc ttttattgaa tctacacttg gtcaaatatt
    28681 caagagagtt gaaaataatg aataccgtgg tggaccagcg tattatattg aatatggtat
    28741 tggtggtaaa tttggtaaaa tttacggaat tatctttgct attgttacga ttatctcagt
    28801 aggtctattg cttcctggtg tgcaatctaa cgctatagca agttctatgc ataatgcgat
    28861 tcatgttcca caatggttaa tgggtggtat tgttgtagtt attttgggat taattatttt
    28921 tggtggtgta cgtagtattg ccaatgttgc aacagccgtt gtaccattta tggcaattat
    28981 ttacatactg atggctgtca ttatcatttg tatcaatata caagaagtgc cagcgttatt
    29041 tgcattaatt ttcaaatcag catttggatt acaatctgct tttggtggta tcgttggcgc
    29101 aatgatagag attggtgtta aacgtggatt atattcaaat gaggctggtc aaggtacagg
    29161 tccacacgca gcagcggcag cagaagtatc acatccaagt aaacaaggtc tagtacaagc
    29221 attttcagtt tatattgata cattatttgt atgtactgca actgctctga ttatacttat
    29281 ttctggtaca tataatgtga ctgatggtac ggttaatgcg aatggcacac cgcatttaat
    29341 taaagatggc ggtatttatg ttgaaaatgc aacaggtaaa gattattcag gtactgcgat
    29401 gtatgcacaa gccggcattg ataaagcgtt ccatggcagt ggttatcaat ttgatcctac
    29461 tttctctggc gtaggttcgt actttattgc atttgcttta ttcttctttg catttactac
    29521 aattttgtcg tactactaca ttacagaaac aaatgttgct tatttaacgc gtaatcaaaa
    29581 taatcaagtt tcatcgatat ttattaatat tgctcgtgtg attattttgt tcgctacatt
    29641 ttacggtgca gttaaaacag ctgatgtagc atgggcattc ggtgatttag gtgtaggtct
    29701 aatggcttgg ttaaatatca ttgcgatttg gattttacat aagcctgccg taaatgcttt
    29761 aaaagattat gaaattcaaa agaaacgttt aggcaacggt tataatgcag tttatcaacc
    29821 tgatccgaat aaattaccta atgctgtctt ttggttgaag acgtatccag aacgtttaaa
    29881 acaagcacgt gccaaaaagt aatctacttt tgtttatagt atatgtagtg atcatttgat
    29941 aaaaaagaaa agtattgaga attttaggtg ctcagaaatt tgaattttaa aaatatagtg
    30001 tctcttggta caataacaat acaactacta ggggcacttt tttatgtcag aatttaaaac
    30061 tggtaagatt aataaacatg ttttatatag taatatttta aatagagatg tcacgttaag
    30121 tatttattta ccagaatctt ataatcaact tgttaaatat aatgtcattc tttgctttga
    30181 cggattagat tttttacgtt tcgggagaat acaacgtaca tatgaatcgt taatcaaaga
    30241 agcgcgtatt gatgatgcga tcattgttgg attccattat gaagacgttg ataagcgtag
    30301 agaggaattt catccacaag gaagtcgttc tcatttaact attcaatcag tcggtaaaga
    30361 aatattgcca tttattgact cgacgttttc tacactgaaa gtaggtaatg caaggttatt
    30421 agtaggggat agtttagcgg gtagtattgc cttattaacg gcgttgacct atccaacgat
    30481 ttttagtcgt gtagcaatgt taagtccaca ttcagatgaa aaagtattag ataagctaaa
    30541 tcaatgtgca aataaagaac aattgacaat ttggcatgtc attggtctag atgaaaaaga
    30601 ttttacttta ccaacaaatg gtaagcgtgc cgatttctta acaccgaata gagaattagc
    30661 tgaacaaatt aagaaatata atataactta ttattacgat gaatttgatg gtggtcacca
    30721 atggaaagat tggaaaccat tgctgtcaga tatattattg tattttttaa gtaaaaacac
    30781 agatgatcaa ctttatgaat aatttacatt agtagattta gtatgaattg tcttcatata
    30841 gtctggtcta taatataatt tataaaagat tttactgttt aatttaattt aaatttgacg
    30901 aaattgcaaa agatgtataa tgaattattt ttaatgtaac ggttttcaaa gaaatttgat
    30961 ataatagcaa taggttaaac aaaggaggaa ttcagatgat tttaggatta gcattaattc
    31021 catcaaagtc atttcaagaa gcggtggatt cttaccgtaa aagatatgat aaacagtatt
    31081 cacgaattaa accacatgtg acaattaaag cgccatttga aattaaagat ggtgatttag
    31141 attctgtcat tgaacaggtt agagctcgta ttaatggtat accagcagta gaagttcatg
    31201 ctacaaaagc ttctagcttc aaaccaacga acaatgtgat ttactttaaa gttgcgaaga
    31261 cggacgactt agaagaattg tttaatcgct ttaatggaga agatttctat ggagaagctg
    31321 aacatgtttt tgtgccacac tttacaatag cacaaggact atctagccaa gaattcgaag
    31381 atatttttgg tcaagtagca ttagctgggg tagaccataa agaaattatc gatgaattaa
    31441 ctttgttacg ttttgacgat gacgaagata aatggaaagt tattgaaacg tttaaattag
    31501 cttaagtaac ataatagtat tgttaatcgt agtatgtttg aattaataag aaaatggtca
    31561 tttttattga atgtaataaa aatgaccatt ttctttattt taaaatacgt tttaacctta
    31621 cttagctttt tctctattta ctataaagta gcttccataa aatacagcta agactaaaaa
    31681 gattaatgcc gagaaataaa atgtattgtt taaattgttg gtaaattgtg taattaatcc
    31741 gccaaataat ggccctatca ttgagccgaa tccttggata ctattaaaaa caccccaagt
    31801 ttcttcttgt tcatctgatt tgataaatcg tgccataaag gtattccatg ctggtaataa
    31861 gatgccatac attagaccga tagctaaagc gataatccac aagatgtgaa tattaacaat
    31921 catagataga gtaaaaatta atatcatgta taaaataaat ccgcttagaa taacaccata
    31981 cataaagttt ctgctgcggt tatctattag tttcgataaa aatagcatcg aaactgcaca
    32041 gccgatacca ccaataatga ttgcaacagt atattcaatt gtgcttacgt taataacctt
    32101 agtagcatat gttggtaata taggaactag ggcagcaatt gcggcacctt gtaaaagaat
    32161 accagggaac aacaataaat ggcgctttgt cacatcaaca atttgtctca attgagcttt
    32221 aactggacga gtattataat ttgttaactt tacatcgaca aaataatata atatccatgc
    32281 aattaaaacg actaaagaca tcatgaaggc aaagcgtgtt gggtgcactt tgataagtag
    32341 attcataaaa accataccta ccaataggcc taacaaccat gaaaaataaa catagcccat
    32401 ttgtttgcca cgtttatctt cttcaacact ggataacata atgacccaaa taggactaac
    32461 tgcaataccg agcatcatag cactaaatat gattacaaaa ggtgatgctg gaaaccaaat
    32521 aactaaaaat aaacttgtaa atgctaaaat aaatccagtc gttaaaacga tttttgtgcc
    32581 gaattttttc agtaaaaatc ctataacaaa gtttgtagat gcatcagcaa taaaatgtat
    32641 tgaaaatgct agagacgtta ttgctacagc aatggatgta actgttggca agaaattaat
    32701 atagcttagg atatacatgc ctctcgcaaa ttccattaaa aataagataa taagcattaa
    32761 aatgaaattt ttatgattag cgtaattatt taacgaagaa tcttgcatat aaaggaacct
    32821 ttccataaat ctcttgtggt tgtgatgaat gaccgattaa atcaagtaag tctcgacata
    32881 ttgtctgtgt agcatactta attttatctt gttccattgt actaatcatg ttagttaatt
    32941 gctcattacc gttagttaaa cttgctacaa tttttattgc ttcttctgga gtatcagcga
    33001 ttttaccaaa acctttttct tcaaagtaaa gggcattttc aagctcttga ccaggtgcag
    33061 gatttaggaa aatcattgga atacaacggg cgaaaccttc agttattgtg ataccaccag
    33121 gtttcgtaat cataagttga cttgatgcca tccattcatt catgtgtttg gtataaccta
    33181 gaatcaatac attctcgtta gatttaaact tagctgttaa agaacgcttt agctctttgc
    33241 tcttaccaca aatcataact acttgtgcat ttgcactttt cgctaatata tcagtaatca
    33301 tcgtgtcaaa acctttagat acaccaaatg caccagctga cattaaaata gtttgcttat
    33361 ctggatctaa gttgttgtct attaaccact gcttttgatt aataggcgtt tcaaatttgt
    33421 tatcaatagg aatacctgtc actttaactg ttgaaggatc aatacctacg tctatgaagt
    33481 cttgtttcgt ttcttttgtt gccacataat atcttgttga atacggcgta atccagtttt
    33541 tatgtaagcg atagtctgtc atcactgtag caactggaat attaatgtta aattgctcag
    33601 ttagtaccga cataactggt gtaggaaacg ttaataatat taaatctggc ttttctttta
    33661 tcaataaatt aattaactta ttaagtccat agtatttgta aaaacatttg tctagtttat
    33721 ctgggcggct gtaataaaac cctttgtaca tatttctaaa atatttaaag ctattgatat
    33781 accatttttt acaaatagaa gtcaaaattg gatgagcttc cataaataaa tcgtgctcaa
    33841 tgacgcttaa atggtctaga ttcatatcat taagttgatt aacgatactc tgtgtaactt
    33901 gcatatgacc gttaccgaat gagccagtaa taatcaatat ctttttattt tgagtaacca
    33961 ttaatagcca ccctccgtta gtttgaaaat tttatttaag tgtaacttat tttacggcat
    34021 tataaaagaa ataaagacgc aaagtcgtta catttatagc aattttaatc tatagatgaa
    34081 ttgatacaaa ataaaacgtt attttataaa gcaatttatt gttctatgtt ttatttgtat
    34141 atttaaaatt atccagtata caattatagc atatttttgg aaacaattat gatattatac
    34201 catgttacaa gatggtttta ataatttaag atgagccata attgtaaaac taattcataa
    34261 taccgtatgt tttattttta atagtagaaa ttagaaaatg ctgattagta ggatataaca
    34321 gtgaaattat aaatttatta acatcaacaa aacgtgtata ataaacatat tgtagaaaaa
    34381 ggagcggttc agtttggatg caagtacgtt gtttaagaaa gtaaaagtaa agcgtgtatt
    34441 gggttcttta gaacaacaaa tagatgatat cactactgat tcacgtacag cgagagaagg
    34501 tagcattttt gtcgcttcag ttggatatac tgtagacagt cataagttct gtcaaaatgt
    34561 agctgatcaa gggtgtaagt tggtagtggt caataaagaa caatcattac cagctaacgt
    34621 aacacaagtg gttgtgccgg acacattaag agtagctagt attctagcac acacattata
    34681 tgattatccg agtcatcagt tagtgacatt tggtgtaacg ggtacaaatg gtaaaacttc
    34741 tattgcgacg atgattcatt taattcaaag aaagttacaa aaaaatagtg catatttagg
    34801 aactaatggt ttccaaatta atgaaacaaa gacaaaaggt gcaaatacga caccagaaac
    34861 agtttcttta actaagaaaa ttaaagaagc agttgatgca ggcgctgaat ctatgacatt
    34921 agaagtatca agccatggct tagtattagg acgactgcga ggcgttgaat ttgacgttgc
    34981 aatattttca aatttaacac aagaccattt agattttcat ggcacaatgg aagcatacgg
    35041 acacgcgaag tctttattgt ttagtcaatt aggtgaagat ttgtcgaaag aaaagtatgt
    35101 cgtgttaaac aatgacgatt cattttctga gtatttaaga acagtgacgc cttatgaagt
    35161 atttagttat ggaattgatg aggaagccca atttatggct aaaaatattc aagaatcttt
    35221 acaaggtgtc agctttgatt ttgtaacgcc ttttggaact tacccagtaa aatcgcctta
    35281 tgttggtaag tttaatattt ctaatattat ggcggcaatg attgcggtgt ggagtaaagg
    35341 tacatcttta gaaacgatta ttaaagctgt tgaaaattta gaacctgttg aagggcgatt
    35401 agaagtttta gatccttcgt tacctattga tttaattatc gattatgcac atacagctga
    35461 tggtatgaac aaattaatcg atgcagtaca gccttttgta aagcaaaagt tgatattttt
    35521 agttggtatg gcaggcgaac gtgatttaac taaaacgcct gaaatggggc gagttgcctg
    35581 tcgtgcagat tatgtcattt tcacaccgga taatccggca aatgatgacc cgaaaatgtt
    35641 aacggcagaa ttagccaaag gtgcaacaca tcaaaactat attgaatttg atgatcgtgc
    35701 agaagggata aaacatgcaa ttgacatagc tgagcctggg gatactgtcg ttttagcatc
    35761 aaaaggaaga gaaccatatc aaatcatgcc agggcatatt aaggtgccac atcgagatga
    35821 tttaattggc cttgaagcag cttacaaaaa gttcggtggt ggccctgttg attaataaaa
    35881 gatttattga tgaaggtaaa actattgatg tttatttatt cgaagcatta aataaccaga
    35941 taatcattgc tataccagat tggttttggt catatcagat ggcaatgaca ttagatgaag
    36001 aaacttgttt tgaagcaata ctcatgcaat tgtttgtttt taaagaagag gaagaggcag
    36061 aatcgattgc atcacaacta acagattgga tagaaacata taaaaaggag aaagactaat
    36121 gaacttaaag caagaagttg agtctagaaa gacttttgcg attatttcac atcccgatgc
    36181 agggaaaaca acgttaactg aaaaactatt gtacttcagt ggtgctattc gtgaagcggg
    36241 tacagttaaa gggaagaaga ctggtaaatt tgcgacaagt gactggatga aagttgaaca
    36301 agagcgtggt atttctgtaa ctagttcagt aatgcaattt gattacgatg attataaaat
    36361 caatatctta gatacaccag gacatgaaga cttttcagaa gatacgtata gaacattaat
    36421 ggcagttgac agtgctgtca tggtcataga ctgtgcaaaa ggtattgaac cacaaacatt
    36481 gaagttattt aaagtttgta aaatgcgtgg tattccaatc tttacattca ttaataaatt
    36541 agaccgagta ggtaaagaac catttgaatt attagatgaa atcgaagaga cattaaatat
    36601 tgaaacatac cctatgaatt ggccaattgg tatgggacaa agtttctttg gcatcattga
    36661 tagaaagtct aaaacaattg aaccatttag agatgaagaa aatatattac atttgaatga
    36721 tgattttgag ttggaagaag atcatgcaat tacaaatgat agtgattttg aacaagcgat
    36781 tgaagaatta atgttggttg aagaagcggg tgaagccttt gataatgacg cgctgttgag
    36841 tggagactta acacctgtat ttttcggttc agctttagct aactttggtg tacaaaattt
    36901 cttaaatgca tatgttgatt ttgcgccaat gccaaatgcg agacaaacaa aagaagacgt
    36961 tgaagtaagc ccgtttgatg attcattttc aggatttatc tttaaaattc aagccaacat
    37021 ggaccctaaa caccgtgata gaattgcctt tatgcgtgtc gttagtggtg catttgaacg
    37081 tggtatggat gttactttgc aacgtactaa taaaaagcaa aagatcacac gttcaacgtc
    37141 atttatggca gacgataaag aaactgtgaa tcatgctgta gcaggcgata tcattggact
    37201 atatgatact ggtaattatc aaattggaga tactttagtt ggtggaaaac aaacctacag
    37261 tttccaagat ttaccacaat ttacgccaga aatttttatg aaagtttctg ctaaaaacgt
    37321 catgaaacag aagcatttcc ataaaggtat tgaacaatta gtacaagaag gtgcgattca
    37381 atactataaa acattacaca caaaccaaat tattttaggt gctgttggtc agttacaatt
    37441 tgaagttttc gaacatagaa tgaaaaacga atataatgtt gatgttgtta tggagccagt
    37501 aggccgtaaa attgcacgtt ggattgaaaa tgaagaccaa attacagata agatgaacac
    37561 atcaagatcg attttagtga aagatagata tgacgattta gtattcttat ttgaaaatga
    37621 atttgcaaca agatggtttg aagagaaatt ccctgaaatt aaattgtata gtttacttta
    37681 acagctcaat tgtataatcg aatttgttac attaaaaata attgtttcgt tgaagaaaaa
    37741 taaattgtat attttaaaag aaaaaggtat actatgatgt atcaaatgaa taacctatgg
    37801 cattttgtca gaggggagta acttaagaat catgaccgta taaatgattc gacactttat
    37861 cgtcattacg aagatatctt ccggtaaagt gggcaattta aattgcttag tgagaccttt
    37921 gctatttatt tagcataggt ctttttgttt gtacttaact tatttattta aaggagttgt
    37981 acatgttaat ggatccaagt ttgatcttac cttatttatg ggtacttgtc gttttagtat
    38041 ttttagaagg cttattagca gcagataacg cgattgttat ggctgtaatg gttaagcact
    38101 taccacccga acaacgtaaa aaagctttgt tttacggttt gttaggtgca tttgtattta
    38161 gatttttagc attattctta attagtatta tcgcgaactt ttggtttatt caagctgcag
    38221 gagcggttta cttaatttat atgtcaatca aaaatctgtg gcagttcttt aaacacccag
    38281 aaattgaaag tcctgaagct ggagatgatc atcattatga tgaatctggt gaagagatta
    38341 aagcaagtaa caaatcattc tggggaactg tgttgaaaat agaatttgca gatatcgcat
    38401 ttgccattga ttctatgctt gctgctttag ctattgctgt aacacttcct aaagttggta
    38461 ttcactttgg tggtatggac ttaggtcagt tcgtagtcat gttcctaggc ggaatgattg
    38521 gtgttattct aatgcgttat gcagcaacat ggtttgtaga gctattaaac aaatatccag
    38581 gacttgaagg tgcagccttc gcgatcgttg gttgggtagg tgttaaatta gttgtcatgg
    38641 tattagcgca cccagacatc gctgtattgc ctgagcactt cccacatggc gtattatggc
    38701 aatctatttt ctggacagta ctaattggat tagtaattat cggttggtta ggttcagttg
    38761 ttaaaaataa aaaatcgcat aaataattga tgtgaagcgg acaatcttaa tttagtttaa
    38821 ggttgtcctt tttcatttaa ttgagtgatt tatgaaaaat ggattttgaa gaatgtgaat
    38881 caaaagatgc gatatagtat taagaaaatg tgccttttat atttagcatt ttttcaatag
    38941 aaattatata gattttaaag caaattaggt gttaatgtgt cataatgata agtgatttta
    39001 ttgaatggag tggacattag tggatattgg taaaaaacat gtaattccta aaagtcagta
    39061 ccgacgtaag cgtcgtgaat tcttccacaa cgaagacaga gaagaaaatt taaatcaaca
    39121 tcaagataaa caaaatatag ataatacaac atcaaaaaaa gcagataagc aaatacataa
    39181 agattcaatt gataagcacg aacgttttaa aaatagttta tcatcgcatt tagaacagag
    39241 aaaccgtgat gttaatgaga ataaagctga agaaagtaaa agtaatcagg atagtaagtc
    39301 agcatataac agagatcatt atttaacaga cgatgtatct aaaaaacaaa attcattaga
    39361 ttcagtggac caagatacag tgaaatcaaa atattatgag caaaattctg aagcgacttt
    39421 atcaactaaa tcaaccgata aagtagaatc aactgaaatg agaaagctaa gttcagataa
    39481 aaacaaagtt ggtcatgaag agcaacatgt actttctaaa ccttcagaac atgataaaga
    39541 gactagaatt gattctgagt cttcaagaac tgattcagac agctcgatgc agacagagaa
    39601 aataaaaaaa gacagttcag atggaaataa aagtagtaat ctgaaatctg aagtaatatc
    39661 agacaaatca aatacagtac caaaattgtc ggaatctgat gatgaagtaa ataatcagaa
    39721 gccattaact ttaccggaag aacagaaatt gaaaagacag caaagtcaaa atgagcaaac
    39781 aaaaacctat acatatggtg atagcgaaca aaatgacaag tctaatcatg aaaatgattt
    39841 aagtcatcat ataccatcga taagtgatga taaagataac gtcatgagag aaaatcatat
    39901 tgttgacgat aatcctgata atgatatcaa tacaccatca ttatcaaaaa cagatgacga
    39961 tcgaaaactt gatgaaaaaa ttcatgttga agataaacat aaacaaaatg cagactcgtc
    40021 tgaaacggtg ggatatcaaa gtcagtcaac tgcatctcat cgtagcactg aaaaaagaaa
    40081 tatttctatt aatgaccatg ataaattaaa cggtcaaaaa acaaatacaa agacatcggc
    40141 aaataataat caaaaaaagg ctacatcaaa attgaacaaa gggcgcgcta cgaataataa
    40201 ttatagtgac attttgaaaa agttttggat gatgtattgg cctaaattag ttattctaat
    40261 gggtattatt attctaattg ttattttgaa tgccattttt aataatgtga acaaaaatga
    40321 tcgcatgaat gataataatg atgcagatgc tcaaaaatat acgacaacga tgaaaaatgc
    40381 caataacaca gttaaatcgg tcgttacagt tgaaaatgaa acatcaaaag attcatcatt
    40441 acctaaagat aaagcatctc aagacgaagt gggatcaggt gttgtatata aaaaatctgg
    40501 agatacgtta tatattgtta cgaatgcaca cgttgtcggt gataaagaaa atcaaaaaat
    40561 aactttctcg aataataaaa gtgttgttgg gaaagtgctt ggtaaagata aatggtcaga
    40621 tttagctgtt gttaaagcaa cttcttcaga cagttcagtg aaagagatag ctattggaga
    40681 ttcaaataat ttagtgttag gagagccaat attagtcgta ggtaatccac ttggtgtaga
    40741 ctttaaaggc actgtgacag aaggtattat ttcaggtctg aacagaaatg ttcctattga
    40801 tttcgataaa gataataaat atgatatgtt gatgaaagct ttccaaattg atgcatcagt
    40861 aaatccaggt aactcgggtg gtgctgtcgt caatagagaa ggaaaattaa taggtgtagt
    40921 tgcagctaaa attagtatgc caaacgttga aaatatgtca tttgcaatac ctgttaatga
    40981 agtacaaaag attgtaaaag atttagaaac aaaaggtaaa attgactatc ccgatgtagg
    41041 tgttaaaatg aagaatattg ccagtctaaa tagttttgaa agacaagcag ttaaattgcc
    41101 aggaaaagtt aagaacggtg ttgttgtaga tcaagttgac aacaatggtt tagcagatca
    41161 atctggtctg aaaaaaggtg atgtaattac tgaattagat ggcaaacttt tagaagatga
    41221 tttacgcttt aggcagatta tatttagtca taaagatgac ttgaaatcaa ttacagcgaa
    41281 gatttataga gatggtaaag agaaagaaat taatattaaa ctaaaataat gttgaggtga
    41341 acaacgtgtc aatttttagc cagtttttaa aaagatcaag ccctcaacaa ggtattgtat
    41401 tgtactatat cgtcgcaatt gtcattgcat ttttattatt aaacttaccg tatgttcata
    41461 aaccaggtgt agaagtaaat ccaattgaca cattatttgt tgccgtatcc ggaattagtg
    41521 ttactggatt gtctccgata agtattgtcg atacctattc tacatttgga caattaatta
    41581 tcctcgtgat attaaatatt ggtggaattg gcgtcatggc aattggtacg atgttatggg
    41641 tggtactagg taaacatatt ggaattagag aacgtcagtt aattatgtta gataataaca
    41701 aaaacacaat gagtggtacc gtcaaattga ttattgatat tgtaaaatca atatttgtaa
    41761 tcgaactcgt aggagccatg ttattagcat tttactttta tcgagataat ccagatttaa
    41821 aatatgcaat catgcaaggt gtttttgttt ctatttctgc cactaccaat ggtggattag
    41881 atattacagg taagtcatta attccttatg cacatgatta ttttgtacaa gcgatagtta
    41941 tatttttaat aattttagga tcaatcggct tcccagtatt attagaagtt aaagcttata
    42001 ttcaaaatag ggttactaat tttagatttt cattatttac taaaattacg acatcaacat
    42061 atttattcct atttattgtt ggggtattag ccattctatt atttgaacat aaccatgcgt
    42121 tcaaaggttt aagttggcat caatcgttat tctattcgct gtttcaatca gcgactacaa
    42181 gaagtgcggg tcttcaaaca attgatgtga caacactaag tgaccccact aatattatca
    42241 tgggtatttt aatgtttata ggatcttcgc caagttcggt tggtggcggt attcgtacaa
    42301 caactttcgc tattttaatt ttgtttttaa ttaactttag taataatgcc gataaaacat
    42361 ccattaaagt ttacaataga gaagtacaca ttatggatat tcaacgttca tttgcagtat
    42421 ttacaatggc gacaatttta acatttttag gaatgctaat tatatcagct actgaaaatg
    42481 gtaagcttac atttttacaa gtattttttg aagtcatgtc tgcatttgga acttgtggac
    42541 tatcgcttgg tgtcacaagt gatattagtg atatttctaa ggtcgtacta atgatactca
    42601 tgtttatagg acgtgttggc ttaatatcat ttatcattat gatagcagga cgtcgagaac
    42661 cagataaatt ccattatcca aaagaacgta ttcaaatagg ataatataat agcaatctaa
    42721 gtttagttaa tgtagatttt aactggaact tagattgctt ttttagtttg tatttttaac
    42781 ttattttata agacgattgg tttcgaaaat ggtaaactag taacaatgag aggtgtaaca
    42841 tgatggaaaa aaatgaaaac attaatgtag agattttaac tacgtcagat atgcatagtc
    42901 atttcttaaa tggtgattat ggttcaaata tttatagagc tggtacttat gttaaccaag
    42961 taagagcaca aaatcatcgc gtcattttat tagatagtgg cggaagttta gctggctcgt
    43021 tagcggccta ttattatgct attgttgcac cttataaacg acatccaatg ataaagttaa
    43081 tgaacagaat gcattatgat gctagcggtg tgagtccaag tgaattcaag tttggtttat
    43141 catttttaac tcgttcaatt gctttggcac gttttccatg gttatcagca aatattgaat
    43201 acaatgttac taaggagcct tatttttcaa ctccatattg tattaaacat tttggtgact
    43261 taaaaattgc tatcgtaggc gtcacagcag atggtttaat ggaaaatgag tattctgaaa
    43321 tggagcaaga tgtatctatt gaaaagacat tagtggcatc aaaacgttgg attagatata
    43381 tccatgaagt tgaagagcca gattttttga ttgtaattta tcatggtgga ttgaataaaa
    43441 ttagtaatag tacgaaaaat aaaaaggcaa gttcgaatga agctgaaaaa ttaatggaag
    43501 aactcggtgt tatagattta atgattacag ctcatcagca tcaaacaata gtaggtcaag
    43561 atcatgaaac gtattatgtt caggctggtc aagatgccaa agagcttgta catctttcga
    43621 ttaattttaa aaagagaaca acaacttatg atgttgaaag cattgattct aaagtgattg
    43681 acttaaatga gtatgaagag gatcaagaat tattagattt aacattctat gatagaaaag
    43741 cagtggctta ttggtcacag gaaatcataa gtgataaagg tttgatgtta tcagtaaatg
    43801 ggttacaaga tttagtctgt caaacacatc cattttcgca attattacat gatgcaattc
    43861 accttgcatt tgataatgat ataacatgtg tccacgtgcc tatgaacgga gagaaggggt
    43921 tgagtggaca gattcgaaat gaagatttgt atcatgcata cccatatcca gataagccaa
    43981 tggatatgac aattagtggt caaaatatca aagatatatt ggagtatagt tattcacatt
    44041 tagattttgt taacgagcaa ttaagcttaa caattattga tgaaacgtta tgtacaatgt
    44101 ggcaaggatt caattatgag attgatatga atcaagaacc tgggcaacga gtaatgttag
    44161 atcaaattga tttgactaag agttatagag ttacaatgac tgactattgt tatcgtaact
    44221 acaagaatta tttaaaaaat gctattatac atgaatcata cgatgaaaca atgagtacat
    44281 taattgcaga gaagttaaga gatccgaatt atcgtatttc atgtagtgat aattttatag
    44341 ttaaaaacag gtaaatattg ctatgtaata aattaattat aagttcaaca agtataaaaa
    44401 cctttcttag agaagattat ctaggaaagg tttttagaat tttaaaattg atataaaaga
    44461 acagtgaaat tctcattcat tatatataag taaacaaaaa agctaaaatg gtatatacca
    44521 ttttagcaag taacggaaac ggagggattc gaaccctcgc gccgctttcg cgacctacac
    44581 ccttagcagg ggcgcctctt cagccaactt gagtacgttt cc
//
LOCUS       DALAGF010000010        44034 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.010, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000010 DALAGF010000000
VERSION     DALAGF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 44034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44034
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.010"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            99..2372
                     /gene="recJ"
                     /locus_tag="O2692_000511"
     CDS             99..2372
                     /gene="recJ"
                     /locus_tag="O2692_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCZ4298156.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTATTILVIT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDVEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     QVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            2394..2912
                     /locus_tag="O2692_000512"
     CDS             2394..2912
                     /locus_tag="O2692_000512"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCZ4298157.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLISYDE"
     gene            3340..5529
                     /locus_tag="O2692_000513"
     CDS             3340..5529
                     /locus_tag="O2692_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCZ4298158.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            5541..5993
                     /locus_tag="O2692_000514"
     CDS             5541..5993
                     /locus_tag="O2692_000514"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCZ4298159.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            5990..6865
                     /locus_tag="O2692_000515"
     CDS             5990..6865
                     /locus_tag="O2692_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCZ4298160.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            7326..8588
                     /locus_tag="O2692_000516"
     CDS             7326..8588
                     /locus_tag="O2692_000516"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCZ4298161.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            8604..10370
                     /gene="aspS"
                     /locus_tag="O2692_000517"
     CDS             8604..10370
                     /gene="aspS"
                     /locus_tag="O2692_000517"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCZ4298162.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            10448..10638
                     /gene="ssrS"
                     /locus_tag="O2692_000518"
     ncRNA           10448..10638
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="O2692_000518"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            10703..10831
                     /locus_tag="O2692_000519"
     CDS             10703..10831
                     /locus_tag="O2692_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298163.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            10831..11604
                     /locus_tag="O2692_000520"
     CDS             10831..11604
                     /locus_tag="O2692_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HCZ4298164.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(11765..13039)
                     /locus_tag="O2692_000521"
     CDS             complement(11765..13039)
                     /locus_tag="O2692_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCZ4298165.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            13124..13546
                     /locus_tag="O2692_000522"
     CDS             13124..13546
                     /locus_tag="O2692_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCZ4298166.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            13646..13828
                     /locus_tag="O2692_000523"
     CDS             13646..13828
                     /locus_tag="O2692_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCZ4298167.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKVIGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            13868..14014
                     /locus_tag="O2692_000524"
     CDS             13868..14014
                     /locus_tag="O2692_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298168.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
     gene            complement(14251..15264)
                     /locus_tag="O2692_000525"
     CDS             complement(14251..15264)
                     /locus_tag="O2692_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCZ4298169.1"
                     /translation="MAIKYSALNLVPIREGEDEQTAINDMVNLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
     gene            15576..16718
                     /locus_tag="O2692_000526"
     CDS             15576..16718
                     /locus_tag="O2692_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCZ4298170.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            16719..17837
                     /gene="mnmA"
                     /locus_tag="O2692_000527"
     CDS             16719..17837
                     /gene="mnmA"
                     /locus_tag="O2692_000527"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCZ4298171.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            18299..18967
                     /locus_tag="O2692_000528"
     CDS             18299..18967
                     /locus_tag="O2692_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCZ4298172.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFFNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            18969..21446
                     /locus_tag="O2692_000529"
     CDS             18969..21446
                     /locus_tag="O2692_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCZ4298173.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSEEVMSDSTKTDTN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            21789..24419
                     /gene="alaS"
                     /locus_tag="O2692_000530"
     CDS             21789..24419
                     /gene="alaS"
                     /locus_tag="O2692_000530"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCZ4298174.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAISGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            24482..24742
                     /locus_tag="O2692_000531"
     CDS             24482..24742
                     /locus_tag="O2692_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCZ4298175.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            24746..25174
                     /gene="ruvX"
                     /locus_tag="O2692_000532"
     CDS             24746..25174
                     /gene="ruvX"
                     /locus_tag="O2692_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCZ4298176.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            25189..25497
                     /locus_tag="O2692_000533"
     CDS             25189..25497
                     /locus_tag="O2692_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCZ4298177.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            25782..26420
                     /locus_tag="O2692_000534"
     CDS             25782..26420
                     /locus_tag="O2692_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCZ4298178.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            26423..27346
                     /locus_tag="O2692_000535"
     CDS             26423..27346
                     /locus_tag="O2692_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCZ4298179.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            27358..28626
                     /locus_tag="O2692_000536"
     CDS             27358..28626
                     /locus_tag="O2692_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCZ4298180.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            28626..29249
                     /gene="udk"
                     /locus_tag="O2692_000537"
     CDS             28626..29249
                     /gene="udk"
                     /locus_tag="O2692_000537"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCZ4298181.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            29277..29753
                     /gene="greA"
                     /locus_tag="O2692_000538"
     CDS             29277..29753
                     /gene="greA"
                     /locus_tag="O2692_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCZ4298182.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            30079..30813
                     /gene="pxpB"
                     /locus_tag="O2692_000539"
     CDS             30079..30813
                     /gene="pxpB"
                     /locus_tag="O2692_000539"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCZ4298183.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     KFIQIERDISDGNFNVDDWVVIENVN"
     gene            30803..31813
                     /locus_tag="O2692_000540"
     CDS             30803..31813
                     /locus_tag="O2692_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCZ4298184.1"
                     /translation="MSIKILQPGLFSTVQDLGRIGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSINASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLADDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            31815..32264
                     /locus_tag="O2692_000541"
     CDS             31815..32264
                     /locus_tag="O2692_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HCZ4298185.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMIGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            32278..33639
                     /locus_tag="O2692_000542"
     CDS             32278..33639
                     /locus_tag="O2692_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCZ4298186.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIENLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKVTLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            33639..34391
                     /gene="pxpA"
                     /locus_tag="O2692_000543"
     CDS             33639..34391
                     /gene="pxpA"
                     /locus_tag="O2692_000543"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCZ4298187.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIANVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            34404..35633
                     /locus_tag="O2692_000544"
     CDS             34404..35633
                     /locus_tag="O2692_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HCZ4298188.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
     gene            36079..36831
                     /locus_tag="O2692_000545"
     CDS             36079..36831
                     /locus_tag="O2692_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCZ4298189.1"
                     /translation="MGEFEVKYLTGFILILLLEGIFTNSASAIEYSDLHHKSKFDSKR
                     LSNAKMSFINPTQLENKNTNDRLLKHDLLFHDMFVNDDWKKDFKVEFENEALSKKFIN
                     KDIDIFAGNYGYGCHGGATNKTQCSYGGVTLSDNNKYDDYKNIPCNLWIDGHQTEIEL
                     TAVKTKKKIVTIQELEVQLRNYLNEKYKLYEQGGDIVKGYVKYYNDDEQNVEYDFYNL
                     NGEYGREVLKMYADNKTINSDKLHLDIYLFKS"
     gene            complement(37033..37302)
                     /locus_tag="O2692_000546"
     CDS             complement(37033..37302)
                     /locus_tag="O2692_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298190.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
     gene            37616..38302
                     /gene="mtnN"
                     /locus_tag="O2692_000547"
     CDS             37616..38302
                     /gene="mtnN"
                     /locus_tag="O2692_000547"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCZ4298191.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            38322..38849
                     /locus_tag="O2692_000548"
     CDS             38322..38849
                     /locus_tag="O2692_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCZ4298192.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            38850..39950
                     /gene="yqeH"
                     /locus_tag="O2692_000549"
     CDS             38850..39950
                     /gene="yqeH"
                     /locus_tag="O2692_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCZ4298193.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            39964..40770
                     /gene="aroE"
                     /locus_tag="O2692_000550"
     CDS             39964..40770
                     /gene="aroE"
                     /locus_tag="O2692_000550"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCZ4298194.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELEGFNITIPHKERIIPYLDYVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            40774..41064
                     /gene="yhbY"
                     /locus_tag="O2692_000551"
     CDS             40774..41064
                     /gene="yhbY"
                     /locus_tag="O2692_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCZ4298195.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATHSELVQVIGSMIVIYRESKDNKEIELP"
     gene            41067..41636
                     /gene="nadD"
                     /locus_tag="O2692_000552"
     CDS             41067..41636
                     /gene="nadD"
                     /locus_tag="O2692_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HCZ4298196.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            41626..42210
                     /gene="yqeK"
                     /locus_tag="O2692_000553"
     CDS             41626..42210
                     /gene="yqeK"
                     /locus_tag="O2692_000553"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCZ4298197.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            42211..42564
                     /gene="rsfS"
                     /locus_tag="O2692_000554"
     CDS             42211..42564
                     /gene="rsfS"
                     /locus_tag="O2692_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCZ4298198.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYSQVAY"
     gene            42567..43283
                     /locus_tag="O2692_000555"
     CDS             42567..43283
                     /locus_tag="O2692_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ4298199.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            43323..44009
                     /locus_tag="O2692_000556"
     CDS             43323..44009
                     /locus_tag="O2692_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HCZ4298200.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTT"
BASE COUNT        16014 a         5944 c         8519 g        13557 t
ORIGIN      
        1 aaaaatggta taattaaagt cttgattatt tagataagtg ctttttattt aaagtaattt
       61 tagatataat gtcttttgga aatgaggcgt aaatatatat gattaaaccg aagtataagt
      121 ggaaattaac taaaccagct gaatacataa gtgatgaatt aacatcaaaa ttaaagttaa
      181 cgccaatcgt aaaaaaaata ttagaaagta aatcgattat tgatgaacaa gcaatagaat
      241 ccatcatttc agatacagat atcaatcatg atgcactgca attgagcgat atgactaaga
      301 ctattgaaag aattaaaaga gcgatagcga atgatgagaa aatcctagtt tatggtgatt
      361 acgatgccga cggtgttacc gcaactacaa ttttagtgat aacattacaa cttttaggtg
      421 cacaagtagg atggcatatt cctaaccgct ttaccgaagg atatggacca aacgaactag
      481 catttagaaa tgctcatgat gaaggcataa cattaatcat aactgttgat aatggcatac
      541 aaggtcataa tgaaattaaa atggttcaag atttaggagt tgatgtcatt gtaactgatc
      601 atcatgaaat tggtagtaca ttaccagaag catatgcaat cgtacatcca atgcatccat
      661 cgtttaatta tccgttccaa caattgtgtg gtgcaggtgt tgcatacaaa ttagcacaag
      721 cattgattga aaatgtacct gattatttta aagcattagt tgcgataggt accattgcgg
      781 atttagtatc cttaactgat gaaaatcgat cattagtgaa acaaggatta aaagtcttaa
      841 atgatcaatg tccgacctct gttaaagcat tgctaaaaga agcgggatac aatgacaata
      901 ttgatgaaga aacaattggt tttattattg gacctagact taatgctgta ggtcgtttag
      961 atgacgcatc attagcttgc gaattgttga tgactgacgt cgaagaagaa gctgcatttt
     1021 tagcagaaca agttgaacat tttaaccgtg aaagaaaaga tattgtagca actatcacag
     1081 aagaagcaat ggctatggca gaaacgaaag ttaaaaaggg agatttattt ttacttttag
     1141 caaaagaaaa ttggcatgaa ggtgtcttag gtattgttgc ttcgaaaatt gtcgaaacat
     1201 ttgctttgcc aacattaatt ttaaatattg atagggagca aaatcatgca aaaggctccg
     1261 ccagatcaat tgatcaagtg tcgatgtttg aaatactatc agcacatcaa gagttaatcg
     1321 ctaaatttgg cggccatcat atggctgcag gtatgacgat ggatattgaa aatatagaat
     1381 cgttagctga aggtttaaat aaatggatga aagaattatc taaaacaact tcgttagatc
     1441 ccgtaaagca agtagatgta ttattaacag aaaatgatat aacgattaaa aatattcgtg
     1501 atatgaatcg tttaagaccc ttcggaacag atttttctag acctattttt gaaatggatg
     1561 atttatcagt ttcttcagtt aaggcgatag gtcaacaaaa aaatcatcta aaattgacac
     1621 ttggtgaaag taatatcgcc gcgctatttt ggcaaaatgg acacttagaa cctgagttac
     1681 aagatgaaca gccaattaat atattaggat ctgttcaaat caacgaatgg aatggtaatc
     1741 aatcaccgca aataattatt caagatattg cgatgaatga acagcaaata ttagattata
     1801 gaagtaagcg aaaaagttta ccttttacag aaaatgatga aaatattgtc gtgcttattc
     1861 atcctaaaag tgataaagta aatgcgaatg aatattatta tggtgaagaa attaaacaac
     1921 aaactgataa agtagtatta agagatttac caacgtcaat ggaagacttg tctaattcct
     1981 tgcaacaact gcaattttct caactttata tagttttgca acataatcat tcgatttact
     2041 tcgatggtat acctaatatg gatattttta aaaagtgtta taaagcatta ataactaaac
     2101 aagaaacaaa tatccagaaa gagggtatgt tattgtgtca acatttaagt gtgaaaccag
     2161 atacacttaa attcatgttg aaagttttct tagacttaaa atttgtaaca caagaagatg
     2221 gtttaattcg aatcaatcaa caacctgata aaagatcgat tgattccagc aaagtatatc
     2281 aattaagaca acaacgtatg gatgttgaaa agcaattatt atatcaagat ttttcagaaa
     2341 taaaaaattg gataaagtca caattgtcgt gagcaattta ggaggaaata ttaatggatt
     2401 taaagcaata cgtatcagaa gttcaagatt ggccgaaacc aggtgttagt ttcaaggata
     2461 ttactacaat tatggataat ggtgaagcat atggctatgc aacagataaa attgtagaat
     2521 acgcaaaaga cagagatgtt gatatcgttg taggacctga agcgcgtggc tttatcattg
     2581 gctgtcctgt agcttattca atggggattg gctttgcacc tgttagaaaa gaagggaaat
     2641 tacctcgtga agtcattcgt tatgagtatg acctagaata tggtacaaat gttttaacaa
     2701 tgcacaaaga tgcaattaaa ccaggtcaac gtgtgttaat tacagatgat ttattagcta
     2761 ctggtggtac gattgaagca gcaataaaat tagttgaaaa attaggcggt atcgtagtag
     2821 gtattgcatt tataattgaa ttgaaatatt taaatggtat tgaaaaaatt aaagattacg
     2881 atgttatgag tttaatctca tacgacgaat aataaataat ataattttat caaatgaaat
     2941 ccttcatcaa atgtataaga accaatgact taattaaaaa agttgtttaa gttttcttaa
     3001 catgagatgt taggattttt tatttactga aaatgttaga tgattgagca ttatacctta
     3061 ataacatcgt ttatttattt cataaattgt agtatcatag aactaatatt taaaaaatga
     3121 aacagtagat ttaggtcgaa tttttgtaaa agttttaaaa gtaggaatag tatacaaatt
     3181 aaactcgctc aagtaaaatt aatattacga ttaatgacga caggataaat atttatcgtc
     3241 gacggacgta tgattggtgt gggacaaata ctattcaaca agagtaccta aatcattgtt
     3301 taaggcgaag taataaatat gaatggggtg tatcatataa tgaacaacga atatccatat
     3361 agtgcagacg aagttcttca caaagcaaaa tcatatttgt cagcagatga atatgagtat
     3421 gttttaaaaa gctatcatat tgcttatgaa gcacataaag gtcagttccg aaaaaacgga
     3481 ttaccataca ttatgcatcc tatacaagtt gcaggtattt taacagaaat gcgattagac
     3541 ggaccgacga ttgtcgcagg ttttttgcat gatgtaattg aagatacacc gtatacattt
     3601 gaagatgtaa aagaaatgtt caatgaagaa gttgctcgaa ttgttgatgg tgtgacgaag
     3661 cttaaaaaag taaaataccg ctcaaaagaa gaacaacaag ctgaaaatca tcgcaagtta
     3721 tttattgcga ttgccaaaga tgtacgcgta attttggtga aattagcaga cagattacat
     3781 aatatgcgta ccttgaaagc catgccgcgc gaaaaacaaa ttagaatttc tcgagaaaca
     3841 ttagaaattt atgcaccatt agcacatcgt cttggtatta atacaatcaa atgggaacta
     3901 gaagatacgg ctcttcgtta tattgataat gtgcaatatt ttagaatagt caatttaatg
     3961 aagaagaaac gtagtgaacg tgaagcgtat atcgaaacgg ctattgatag aatacgtact
     4021 gaaatggacc gaatgaatat cgaaggcgat ataaatggta gacctaaaca tatttacagt
     4081 atttatcgga aaatgatgaa gcagaaaaaa caatttgatc aaatttttga tttgttggcg
     4141 atacgtgtta ttgtcaattc tattaatgat tgttatgcga tacttgggtt ggtgcatacg
     4201 ttatggaaac cgatgccagg acgttttaaa gattatattg caatgcctaa acaaaatttg
     4261 tatcagtcat tgcatactac agtagtaggc ccaaatggag acccgctcga aatccaaata
     4321 cgaacgtttg atatgcacga aattgctgag catggtgttg cagcacactg ggcttacaaa
     4381 gaaggtaaaa aagtaagtga aaaagatcaa acttatcaaa ataagttaaa ttggttaaaa
     4441 gaattagctg aagcggatca tacatcgtct gacgctcaag aatttatgga aaccttaaaa
     4501 tatgacttac agagtgacaa agtatacgca tttaccccag cgagtgatgt tattgagttg
     4561 ccatatggtg ctgtgccgat tgattttgct tatgcgattc acagtgaagt aggtaataag
     4621 atgattggtg ccaaggtgaa tggcaaaatt gtaccaattg actatatttt acaaacaggc
     4681 gatattgttg aaatacgtac tagtaaacat tcatatggac caagtcgtga ttggttgaaa
     4741 attgttaaat cgtctagtgc caaaggtaaa attaaaagtt tcttcaaaaa acaagatcgt
     4801 tcatctaata ttgaaaaagg ccgaatgatg gttgaagctg aaataaaaga gcaaggattt
     4861 agagtcgaag atattttgac agagaaaaat attcaggttg ttaatgaaaa atataacttt
     4921 gcaaatgaag atgatttatt cgcagctgta ggatttggcg gcgtgacatc cttacagatt
     4981 gttaataaat taactgaaag acaacgtatt ttagataaac aacgtgcttt aaatgaagca
     5041 caagaagtta cgaaatcatt gcctattaaa gacaacatca ttactgatag tggtgtctat
     5101 gtagaaggtt tagaaaatgt acttatcaag ttgtcaaaat gttgtaatcc tataccaggt
     5161 gatgatattg taggttatat caccaaaggt cacggtatta aagtacatcg cactgattgc
     5221 ccaaatatta agaacgaaac tgaacgacta attaatgttg aatgggtaaa atcaaaagac
     5281 gcaactcaaa aatatcaggt tgatttagag gtaactgcgt atgaccgaaa tggcttgttg
     5341 aatgaagtac tacaagctgt tagctcgaca gccggcaatt taattaaagt ttcaggacgt
     5401 tcagatattg ataaaaatgc aataataaat attagtgtca tggtgaaaaa cgtgaatgat
     5461 gtttatcgtg tggtagaaaa gatcaaacaa cttggtgatg tttatacagt aacaagagtt
     5521 tggaactaga ggtgcaaaat atgaaagtag ttgtacaaag agttaaagaa gcatcggtga
     5581 cgaatgatac attaaataat caaatcaaaa aaggatattg tttattagtc ggtatcggtc
     5641 agaactctac agagcaagat gcagatgtaa ttgcaaagaa aattgctaat gcaagattat
     5701 ttgaagatga caataataaa ttaaacttta atatccaaca aatgaatggt gaaatactat
     5761 cagtttcaca atttactctc tatgcagatg taaaaaaagg taaccgtcca ggtttctcaa
     5821 attctaaaaa tcctgatcaa gcggtaaaaa tttatgagta ttttaatgat gcgctacgag
     5881 cgtatggtct tactgtgaaa acaggtgaat ttggaacaca catgaatgtt agcataaata
     5941 atgatggtcc agtcactatt atttatgaaa gtcaggacgg caaaattcaa tgaaaaaaat
     6001 agaggcatgg ttatctaaaa agggtcttaa aaataaacgt actctaatag tagtgattgc
     6061 ctttgtctta tttatcatct ttttattttt attgctgaat agcaatagtg aagatagtgg
     6121 gaacatcacg ataactgaaa atgctgaatt acgtacaggt ccaaacgctg cgtatccagt
     6181 catatataaa gttgaaaaag gtgaccattt taaaaagatt ggtaaagtag gtaaatggat
     6241 tgaagttgaa gatacatcca gtaatgaaaa aggttggata gctggatggc acacaaattt
     6301 agatattgtc gcggataata cgaaggagaa aaatcctttg caaggtaaaa caatagtgct
     6361 tgatcctggt catggaggta gtgaccaggg tgcttcaagc aatactaaat ataaaagttt
     6421 agaaaaagat tatacgttga aaacagcaaa agaattgcag cgtactttag aaaaagaagg
     6481 cgcaactgtt aagatgacaa gaacagacga tacatatgtt tcactagaaa atcgtgatat
     6541 caaaggcgat gcctatttga gtatacataa tgatgcgtta gaatcatcta atgcaaatgg
     6601 aatgacagtt tattggtatc atgataatca aagagcttta gcagatacgt tagacgctac
     6661 gattcagaag aaaggtctac tttctaatcg cggttcaaga caagaaaatt atcaagtgtt
     6721 aagacaaaca aaagttcctg ctgttttatt agaattaggt tatattagta acccaactga
     6781 tgaaacgatg attaaagatc aattacatag acaaatttta gaacaagcaa ttgttgatgg
     6841 ccttaaaatt tatttttctg cgtagggctt gcaaaaatat gtgaaagtag ttatcattga
     6901 tattgaattt tataactaaa accgttagta ttcttgaaat ggtaaatgaa ataggtagca
     6961 atctaactaa gattgtgtag gaatataatc catagactga aagattatgc tgagtagttt
     7021 atatacattg aacacaagaa gaggtgcttt atgaaaagta aagccgttaa acgtacgtta
     7081 aacgttttga gtgggtttat taaatgcacg cttataaaaa gtaatgatga ttacaattag
     7141 gcatgttttt taaaccaatg agggtggcaa cacggatatt cgtcccttgt gcagtttcag
     7201 gctgtgcaag ggctttttct tttgctttta agattatgat ttatcgtgca aagttaagtg
     7261 gtcgtatata gttttagttt taaaaaggta attaaataaa atagtttgcc gagggagatg
     7321 tcaaaatgat taaaatacct agagggacgc aggatatttt acctgaagat tcaaagaaat
     7381 ggcgttacat tgaaaatcaa ttagatgaat taatgacatt ttataattat aaagaaataa
     7441 gaacaccaat ttttgaaagt acagatcttt ttgcaagagg tgttggtgat tcaaccgatg
     7501 tcgtacaaaa agaaatgtat acatttaaag ataaaggcga tagaagtatt acattaagac
     7561 ctgagggaac agctgcagtt gtgcgttcat atattgaaca taaaatgcaa ggtaatccaa
     7621 accaaccaat taaactttat tacaatggac cgatgtttag atatgaacgt aagcaaaaag
     7681 gacgctatcg tcaatttaat caatttggtg tagaagctat tggtgctgaa aatcctagcg
     7741 tagatgcaga agtattagct atggttatgc atatttatca atcatttgga ttaaaacatt
     7801 taaagcttgt tattaatagt gtaggggata tggcgtctcg aaaagaatat aacgaagcgt
     7861 tagtgaaaca ctttgaacca gtaattcatg aattttgttc agattgtcaa tcacgtttgc
     7921 atacaaatcc gatgcgaatt ttggattgta aagtagaccg tgataaagaa gcgattaaga
     7981 ctgcacctag aatcactgat ttcttaaatg aggaatctaa ggcatattat gaacaagtaa
     8041 aagcttattt agatgattta ggtattccat atattgaaga tcctaactta gttcgtggat
     8101 tggattatta tacacataca gcatttgaat taatgatgga taaccctaac tatgatggtg
     8161 ccattacaac gctttgtggt ggtggccgtt ataatggttt attagaattg ctagatggtc
     8221 caagtgaaac aggtattggt tttgcgctaa gtatagaacg attattgctt gcacttgaag
     8281 aagaaggtat cgaattagat attgaagaaa acttagattt attcattgtt acaatgggtg
     8341 atcaagcaga tcgatatgct gtgaagctat taaatcattt gagacataat ggtattaaag
     8401 cagataaaga ctatttacag cgtaaaatta aaggacaaat gaaacaagca gaccgtttag
     8461 gtgccaagtt tacaatcgtt attggtgatc aagaattaga aaataataaa atcgatgtta
     8521 aaaatatgac aactggtgaa tctgaaacaa ttgaattaga cgcattagtc gaatatttta
     8581 agaagtagag agggcgttaa aatatgagta agagaacaac ttattgtgga ttagttactg
     8641 aggcattttt aggacaagaa attacattaa aaggatgggt taacaatcgt cgtgaccttg
     8701 gtggattgat tttcgttgat ttaagagata gagaaggaat tgtacaagtc gtgtttaatc
     8761 ctgcattttc agaagaggca ttgaaaattg ctgaaacagt acgttctgaa tatgttgtag
     8821 aagttcaagg tacagttacg aagcgtgacc ctgaaacagt taatcctaaa attaaaactg
     8881 gccaagttga agtacaagtt acaaatatta aagtgattaa taaatctgag acaccaccat
     8941 tttctataaa tgaagaaaat gttaacgttg atgaaaatat tcgattaaaa taccgttatt
     9001 tagatttacg tcgtcaagag ttagcgcaaa catttaaaat gagacatcaa attacacgtt
     9061 ctattcgtca atatttggat gatgaagggt tctttgacat cgaaacacca gtactaacga
     9121 agtcaacacc tgagggtgca cgtgactatt tagtaccatc tcgtgttcat gatggtgaat
     9181 tttatgcatt accacaatca ccacaattat ttaagcaatt attgatgatt agtggatttg
     9241 acaaatacta ccaaatcgta aaatgcttcc gtgacgaaga tttacgtgca gatcgtcaac
     9301 ctgaatttac acaagtcgat attgaaatga gttttgtaga ccaagaagat gtgatgcaaa
     9361 tgggtgaaga aatgcttaaa aaagttgtta aagaagttaa aggcgttgaa attaatggcg
     9421 ctttcccacg catgacatat aaagaagcga tgcgtcgcta tggttctgat aaaccagata
     9481 cacgttttga aatggaatta attgacgttt ctcaattagg acgtgatatg gactttaaag
     9541 tatttaaaga tactgttgaa aatgatggtg aaattaaagc aattgtcgct aaaggtgcag
     9601 ctgaacaata tactcgtaaa gatatggatg ctttaacaga atttgtaaac atctatggtg
     9661 ctaaaggatt agcgtgggtt aaagttgtgg aagatggttt gacaggtcca attggacgtt
     9721 tctttgaaac agaaaatgtt gaaacattac ttacattaac tggtgctgaa gctggtgact
     9781 tagtaatgtt tgttgcagac aaaccaaatg tcgttgcaca aagtttaggt gcattacgtg
     9841 tcaaattagc taaagaatta ggtttaatcg atgaaacaaa attaaacttc ttatgggtga
     9901 cagattggcc attattagaa tatgatgaag atgcgaaacg ttacgttgca gcacatcatc
     9961 catttacatc tccaaaagaa gctgatattg ctaagcttgg cactgcgcca gaagaagctg
    10021 aggcaaatgc ttatgacata gtattaaatg gttatgaatt aggtggcggt tcaatcagaa
    10081 ttcatgatgg tgagttacaa gaaaaaatgt tcgaagttct tggatttact aaagaacaag
    10141 cacaagaaca gttcggcttc ttactagatg catttaaata tggtgcacca ccacatggcg
    10201 gtattgcatt aggattagac cgattagtca tgttattgac taatagaaca aacttgagag
    10261 atacaatcgc attccctaaa acagcatctg ctacatgttt attaacgaat gcgcctggtg
    10321 aagtttctga taaacaatta gaagaacttt ctttgcgaat tcgtcactaa attttaaaaa
    10381 atcaataaac tttggtttgt tataatttca ttaattttaa ttatgtggta atataacatc
    10441 ataagatagt catctgagtt gttcgtaagt ttgcttttta atttgggcct aacactcttt
    10501 gatcaaggga gcccaatggg ttttcttgca gcgtacacgc cttatatgaa ggacgtacaa
    10561 aacaagaaac aggacaccca cctgtatata acaggccgaa tgatcaagct attataacta
    10621 cggcaatacg gactctatcg atacgcaaga ctttgtcttg cgtattttta tgcgtttctt
    10681 taagttttta ataaagaatt agttgggttt tatagcgttt aataaatgtt tcctcgttga
    10741 attttgggaa aataatcaaa gtgataatgc taaatataaa attttaagtt actatgtact
    10801 tatgtataaa tcagtgaaag aggtaaaata atgaaacatc aattttcaag aaatgaacta
    10861 gcgattggac aacaaggttt agatttattg aagcaacaaa ctgttgtcat acttggcgtt
    10921 ggaggtgttg gttcatttgc tgctgaagca ttagcaagaa cgaatattgg tcatattata
    10981 ttaatagata aagatgatgt tgatattaca aatgtaaaca gacaattaca cgctttaact
    11041 acaacggtag gacaaagtaa agtttctttg atggaagaac gtattaaatt aattaatcca
    11101 gattgtaaag tcacgccctt acatatgttt tatacagaag agacatatga agaaattttc
    11161 gataactacg atatagatta ttttgtagat gcaagtgata caataatgta caaagtacac
    11221 ttaatgaaag agtgtcttga ccgagggatt aaagtgattt ccagtatggg tgctgcaaat
    11281 aaaacagatc caacacgttt tacaatttca gatatctcta aaacacatac cgatccaatg
    11341 gccaaaatta ttcgtcaaaa attgaaaaaa ctaggaattc gtaaagggat tccagttgta
    11401 ttttcagatg aaagtccaat tgtcataaga gaagatgtaa aagatatagt tggagataaa
    11461 aatgctatca atcgaaaagg acaaatgcca ccttcttcaa atgcctttgt gccaagtgtt
    11521 gttggattaa tttgtgcaag ttatgtggtg aatgacgtat taaaagatat tccagttcgt
    11581 cgcattaaag acaaagggca ataattcatt ttgaaaggga taattttcaa cgtaaggcaa
    11641 gtgtaaccac accataaaac taaaactgac tagttcgcaa gcatagtaga atatgcctcg
    11701 tgtactagtc agttttgatt tgattatagc taaaatatca aaggtaatcg atttaatctt
    11761 atttttacgg cctttgttta agtaagttgt tatatatttc tttgagttgt tgttcacttt
    11821 tagatgtcgt ttttggttcg taataaattt tgttttttag tttatcaggt aaatattgct
    11881 gtgaaacata gccattaaca tattggtgtg gatatttgta accaatagat cggcctagat
    11941 ctttagcacc ttgataatgt ccatctttta aatggtttgg aatttggccc acatgaccgt
    12001 ttctaatatc ggacaatgca ctatcaatgg cactcattgc tgaatttgac ttaggtgata
    12061 agcatagttc gattactgct tggcttagtg gaattctagc ttctggtaaa cctagacgtt
    12121 ctgctgattc aatagcagca agtgttctct gaccagcatt aggtgaggct aagcctatat
    12181 cctcatagct aattacaagt aatcgtcgaa ctattgtagg taaatctcca gcttcaatta
    12241 atcgtgctaa ataatgtaaa gcggcattga cgtcgctacc acggatagat ttttggaaag
    12301 cgctcataac atcgtaatgc atgtcaccat ccttgtcact tacaaatgca cctttttgta
    12361 aacagtcttt agcatcttgc aatgtaacat gtcgataacc gtctttgtca ttatctgcgc
    12421 ttaatacagc taattccaat gcatttaacg cactacgaac atcaccttgg ctttgtgtag
    12481 aaaagtaggt catggcatct tcatcaattt tgggttgata tgttttcaaa ccattctcat
    12541 catcttctat tgcacgagtt aacgcttggc gcacatcttc gtcatttaaa ggatataact
    12601 cgaaaatttg cgctcttgaa cgaatcgctg gattgatagc atgataagga tttgaagttg
    12661 tagcaccgat caagacgatt ttgccatttt ctaaatgagg taataaaaag tcttgtttag
    12721 ctttatctag tcgatgtatt tcatctaata acaagataac ttgaccagac attttagctt
    12781 cttcaacaac aagttgcata tcttttttag tgttagttac agcattcaat tgtctgaatt
    12841 tatattgcgt actgcccgaa attgctttgg caatacttgt tttgcctata ccaggtggac
    12901 cataaaaaat cattgaagtt aatttttttg tatcaaccat tcttctgata atgcctcttg
    12961 gtccaactaa atgttgttgg gaaatgattt catctatatt ttttgggcgc attctcgatg
    13021 ctaatggttc tgtactcaca tttgtcacac ctttcttata ctatcttaac tcaaaaaatg
    13081 ctaaaatggg aatatcaata ttcgaattaa gtgaggttat acaatgaaaa tttctactaa
    13141 agggagatat ggacttacat tgatgatttc tcttgctaaa aaagaggggc aaggatgtat
    13201 atcattaaag tcaattgctg aagaaaataa tttgagtgat ttatatttag aacagcttgt
    13261 aggtccttta agaaatgcgg ggttaattcg aagtgtacgc ggtgctaaag gtggatacca
    13321 attaagagtg ccagcggaag aaatctcagc aggggatatt ataagactgt tagaaggtcc
    13381 aattacattt gttgaaagta ttgaatcaga accacctgcg caaaaacaac tatggattcg
    13441 catgagagat gcagtgagag atgttttaga taatacaaca ttgaaatatt tagcggaata
    13501 tgtagataca agtgaagatt tagacggata catgttttat atttaatcgt ataaatacta
    13561 tactataaca taaaaacttc atattataat gtttagcgaa cctccttagt ggtatataaa
    13621 tatatacatc caaggaggtt tcactatggc agacgaaagt aagttcgatc aatttaaagg
    13681 aaatgttaaa gaaacagtag gtaacgttac tgataacaaa gaattagaaa aagaaggtca
    13741 gcaagataaa gtgattggta aagcaaaaga agttgttgaa aatgctaaaa acaaaataac
    13801 tgatgcaatt gataaactta aaaaataagt tcaattaaac taatcaaata taagaggagc
    13861 gattaatatg agttttatgg ataaagctaa agacgcagta gaaaaattta aaaatagcga
    13921 taatgaacaa gttaaaaatg taaaagataa gataaatgaa tatacaggat cgaataacga
    13981 agagaaaaaa gaaaatgaag ataaagagaa ataaatttct ttaaattttg gatatgcttt
    14041 tcaatatttt gaaagcgccc catcaaataa acacttacac agtgtaaagt tgaaggggcg
    14101 ctttcatatt tgcattagaa ttttaagtga atttgaatca ttactaaaat aaatgattga
    14161 acaataaaga catctttcat tcaaaatgct gttactgtat atttttatta atattatgga
    14221 tacatacttt cacttagttt atttatcaca ttataaagtg tttttttcat ttatctgatt
    14281 tataacattc ttgaaccgac gataagagtc taattgcata tctttatcat aaatataact
    14341 tattgccata atttcgttga cttcaccata agtttccata aaatcttgaa ttttttgttg
    14401 aacagtattt tctgaaccta ttaatgatat tttctgtctt tccatagcca tcgcgtattc
    14461 tctcggcgtt aatatatttt gtagttcatg tgttggcggt tgaacgggct gcattctgcc
    14521 acgagtgata ctaaccatta cttgtgccat agatgaagct aaatattgtg cttcgtcatc
    14581 attttcagca acgattgtat ttaaacatac aataacataa ggttcgtcta atacatcaga
    14641 aggttcaaat aacgttttgt aaatttcgat agcttctttc atttgttgag gtgcaaaatg
    14701 tccagcgaac acatatggaa gccctttgcg agcagctaaa tgtgcagaat ctgttgaaga
    14761 accaagaatg tataaaggca catttttacc tactgctgga taagcacgaa catatgcttg
    14821 ttggtgagca gggccgaaat attgttgtaa taatgaaacc tcttctggaa atttataaac
    14881 accatcatgt tgatctcgtc ttaatgcact tgcggtcatc atatctgttc caggtgcacg
    14941 tcctaatcct aaatcgacac gatttggaaa taatgttgcc atcgtgccaa attgttccgc
    15001 aacgattaat ggagcatgat taggtaacat gatgcctcca gaacctacac gtatgtgttt
    15061 cgtatgttct aacgtatgtt gaattaataa agcagttgct gaacttacta ggttgggagc
    15121 gttatggtgt tcagcaatcc aatatctttc atatgataat tcgtctaaat gttgtgcgag
    15181 attaaccata tcattaattg ctgtttgttc atcttcacct tctcgaatag ggacaaggtt
    15241 taatgctgaa tatttaatcg ccatttttat atcgtccttc cttttttatg atataagaag
    15301 tataccaatc gaataaccag ataaatatta aattgctcat aaccaaaata atagtacgtt
    15361 acattttaca aataaaaaaa tatctgcaaa tactaaaatt tataaaaaag acaagaataa
    15421 agcgataaac ttaaaaaagc ctatcgaaaa gcatgtgtag aaaatggtaa aatataatta
    15481 aaaacattat aaataaagtt aaacagaaaa gtagaaaaat agtatgtaag gtgctattat
    15541 tggaacattg ataaaattgt gaggagtgtc caaacatgga aatttatgca gattatgctg
    15601 cgaccacacc agtaaaacct gaagtagtag atgcgatgat gacgatttac aattcgcatt
    15661 atggtaatcc atcatcaata catgctaagg gaagagatgc gcgtaaatat ttagatgagt
    15721 caagacgtca aattgcacaa ttacttggtg cagatacaca tgaaattata tttacgagtg
    15781 gcgcaacaga atcgaataat acggcaatta aaggcatagt aaaagcaaat gaacaattgg
    15841 gtaatcacat tatcacatcg aagattgaac atcattctgt gttacatgtt tttgaacaat
    15901 tagagcgaga aggttttgat gtaacatatc tagatgttga tgatacaggt gctattgatt
    15961 tagatcaact tgaagaaaca attacagata agacgatttt agtatctatt atgtttgtaa
    16021 ataatgaagt tggcacagtg caacaaattt atgatattca agatattatt gctgagacaa
    16081 atgcatattt ccatgttgat gcggttcaag caattggaca tcttgatgtt aagtttgacg
    16141 aatttgagat cgacgcaatg agtattactg ctcataagtt tggcggacca aaaggtgtcg
    16201 gtgcattact agttaaagac catgttacac tagattatcc tcaacttggc ggtgaacaag
    16261 aattgaaacg ccgtgcaggc actgaaaatc ttgcgcaaat tgttgggatg gctaaagcat
    16321 tacaattagc agaaaagaat cgagacgata acaatattca tttaatgaat ttaaaagaac
    16381 aatttttagt taaattacaa gaaagagcga ttccatttga attaaatggt tcaatgacgg
    16441 atgctacagg ccatattgta aatctatatt ttccgtttgt tgaagttgaa acgatgttaa
    16501 cattgttgga tatggcacaa atctatgtgt cgtctggatc tgcatgtaca gcaggctcta
    16561 cacaaccgtc gcatgtttta gatgcaatgt tcgaagatga ggagcgatca aatcattcga
    16621 ttcgatttag ttttaacgaa ttgactactg aaaatgaaat taatgcaatt gtagctgaaa
    16681 ttcataaaat atattttaaa tttaaggagg agtcataatt gtcaaataaa gatatacgtg
    16741 ttgtcgttgg tatgtcaggc ggtgtagata gttctgtaac agcccacgtc ttaaaagaac
    16801 aaggttatga tgtcattggc atatttatga aaaactggga tgacactgac gaaaatggcg
    16861 tatgtactgc aactgaagat tacaacgatg ttattgaagt gtgtaatcaa attggcattc
    16921 cgtattacgc tgttaatttt gaaaaagaat attgggataa agtctttacg tatttcttag
    16981 atgaatacaa aaaaggtcgt actccaaatc cagacgttat gtgtaataaa gaaattaagt
    17041 ttaaagcctt tttagatcat gcgatgaatt taggtgcaga ttatgtagca acaggacatt
    17101 acgcacgcat acatcgtcat gaagatggtc atgttgaaat gttacgtggt gtagataata
    17161 ataaagatca aacatacttc ttgaatcaat tatctcaaca acaactttca aaagtgatgt
    17221 tcccaattgg cgacatcgaa aagagtgaag tgcgtcgaat tgctgaagaa caaggacttg
    17281 ttactgctaa gaaaaaagat tctacaggca tttgttttat cggcgaaaaa aactttaaaa
    17341 catttttatc acaatattta cctgcacaac cgggtgatat gataacactt gatggtaaga
    17401 aaatgggtaa acatagtggt ttgatgtatt acacaatagg acaaagacat ggattaggta
    17461 taggtggaga tggcgatcct tggtttgttg tcggtaaaaa cctaaaagat aatgttttat
    17521 atgtagaaca aggattccat cacgatgcat tatacagtga ttacttaatt gcttcagact
    17581 attcatttgt aaatccagaa gataatgact tagatcaagg ttttgaatgt acagctaaat
    17641 ttagatatcg ccaaaaagat acgaaagttt ttgtgaaacg tgaaaacgac catgcactac
    17701 gtgttacttt tgctgagcca gtaagagcaa tcacacctgg acaagcagtt gttttttatc
    17761 aaggtgatgt gtgtcttggt ggtgcaacaa ttgacgatgt gttcaaaaat gaaggtcaat
    17821 taaattatgt tgtataaaca atggcaacaa taaattactt atttgaagtt tcaacgttga
    17881 aaatgacgaa agacagtttt tgatgagaat aattcatgag gatagagtct gggacatcac
    17941 aatgtcctag gctctacaat gttatattgg cgggaccaca acatagagaa tttcgtaaag
    18001 aaattctaca ggcaatgcca gttggggata acgaatttaa ttttgttaaa atatcatttc
    18061 tgtcccactc cctatgcatg aatctaatta tgtattctta tttttaagta cataatagtg
    18121 gtggctaatg tggaagaacc attacataat aaaccgttaa tggttcttaa gcatttctat
    18181 tccattcccg ctttttcatg aatgaagatg atattagatt atattttatt cgttgttaag
    18241 tgattcgaga catacaattt atcaagatgt tataattgat gagaaatgag gttcgtaaat
    18301 gatagatcaa caaacaattt atcaatacat acaaaatgga aaaatagaag aagcgttaca
    18361 agcattgttc ggaaatatcg aagaaaatcc tacaattatt gaaaattata ttaatgctgg
    18421 tatcgtactt gctgatgcga atgagattga aaaggcagag cgtttttttc aaaaagcttt
    18481 aacaatagat ccgaagaatg gcgtcgtatt ttttaatcta gcaaatgtat attataatca
    18541 gcaacgttat caagaagcta ttaaattata tcaacaagca ttacaaacag agattgaaca
    18601 agttgattgt aattatatga tcggtatggc gtttaatcag ttagaatcat ttaagctggc
    18661 attgccgtat ttaatgactg ctgcggaact agataaagac aaagatgcag aagttcaatt
    18721 tcaatatggt cttgtattat gtcaattaga aatgtttaat gaagccataa ctcaacttaa
    18781 acatgtatta acgattgata aaaatcatgt tgatgcaaga tacaatttgg gcttagcgtt
    18841 atttatgaaa aatgaagata ttgatgaagc aataactcat tttaaagaag ctgtgactat
    18901 cgaccctaaa cacttattaa gtcagcatgc gctgaaaaca ttcactaaaa tgaaagagga
    18961 ggagtaacat gtcagaccct acactatttg attattcaat gatcaaaggt actgttgaag
    19021 cgatattgtt tcaaaacagt gataattttt atacagtgct taaagtagat actattgaaa
    19081 caaatgaaga ctttgataca atgccaactg ttgtagggtt tcttccgaat attgttgaag
    19141 gcgatgtcta cacatttaaa gggcaagttg ttgatcatcc acgttatggt aaacaattaa
    19201 aagcagagac ttttgaaaaa gaaatgccac aaacgaaaga agcaattata agttatttat
    19261 ctagtgattt gtttaaaggt gtaggtaaaa agacagctca aaatattgtt aatacattag
    19321 gtgacaatgc gataaatgat atattagatg accattcagt gctagaaaaa gtttcgggat
    19381 tatctaaaaa gaaacagaaa caaattgcag aacaaatttc agcaaatcaa gaatctgaaa
    19441 aaataatgat tcgtttacat gatttagggt ttggtcctaa actttcaatg gcaatttatc
    19501 agttttatct cggcgataca ctgactattt tagatcgaaa tccatatcaa ttgatttatg
    19561 atattaaagg tattggtttt aataaagcag atcaacttgc tagaaatatt ggtatcgctt
    19621 ataacgataa tgaacgatta aaagctgcgt tgctctatac gttagaagaa gaatgtatta
    19681 aacagggaca tacatatttg cctatcaatg ttgttataga tttaacggtt gatgtattaa
    19741 attatcaaga tgaagaggtc atcgaacctg aaaaattaga cgaaatgttg caatacttaa
    19801 atgaggaaaa acgactaatt attgacaatg aacaagttgc aattccaagt ttgtattatt
    19861 ctgaaatcaa aagtgttcaa aacttattca gaataaagac gcatacaaat aaacttacag
    19921 aaattgaaca gtcagattta caaatgcata ttggtgaaat agaagatgcg aatcaagtga
    19981 actatgcagc ttcacaaaaa gaagctttgc aaacggctat caattctaag gtgatgctat
    20041 taaccggtgg accgggaaca ggaaaaacga cagtaattaa aggtattgtt gaattatatg
    20101 ctgagattca tggcttatca ttagattatg atgattatgt caatgatgat tatccagttg
    20161 tattagcagc gccaacagga agagcgtcta agagactaca agagtcaact ggattagaag
    20221 caatgacgat tcatcgttta ataggttgga atcaagacac aaagccagag gatatattag
    20281 aaaatgaaat aaacgcacga ctcataatca ttgatgagat gtctatggta gatacttggt
    20341 tgttccacca atttttaagt gcagttcctt tagatgcaca attgatattt gtaggtgatg
    20401 aggaccaatt gccttctgta ggtcctggtc aagtatttaa agatttaatt gaatcgaaag
    20461 ctataccacg agtaaatctg actgaagtat atcgtcaaca agatggttca agcattatag
    20521 aattagcaca tcgaatgaag ttgggtcaga aaattgatat tacacagcgt tttcatgata
    20581 gaagttttat taactgtcag gcaaaccaaa taccaacagt tgttgagaag gtagttacta
    20641 gtgctgttaa taaagggtat actatggctg atattcaagt gcttgcacca atgtataaag
    20701 gaaatgccgg tattaaacgt ttaaatcaag ttttacaaga tattttaaac cctaaaaaga
    20761 aagacacacg agaaattgag tttggtgatg tagtctttag aaaaggtgac aaagtattac
    20821 aacttgttaa taggccaaat gataacatat tcaatggtga cataggagtt attgttggta
    20881 ttttttgggc gaaagaaaat gcccttaata aagatgtact tgttgtggat tttgaaggta
    20941 atgaaattac atttacgaaa caagatatga tggaattaac acatgcttat tgtacatcaa
    21001 tccataaatc acaaggttca gaatttccta ttgttatcat gcctattgtt aaacagtatt
    21061 ttagaatgtt acaaagaccg atattatata ctggtttaac tagagcgaaa acgagtttag
    21121 ttttacttgg tgatccggaa gcatttgata ttggattgaa aacaaatggt caagcgcgtt
    21181 taacacaatt atgtacattg ttgaaaaatt attttaattc agaagatgaa gaaatgctgg
    21241 aaaacacagc aactaatgat acaggagctt cacaaacgac aatagatgat caagtagaag
    21301 cgccaatgtc atcgaatgat agtgaagaag ttatgtctga ttcaacaaaa acagatacaa
    21361 atgtattaac ggaagctaca atgtttaaaa ttgacccaat gattaatatg ggagaaatca
    21421 cgccgtatga ctttatagaa cgttgacaac aagcgtaaat gtaactaaaa tagaattaaa
    21481 ttcgtataaa gatgagtagt taatgatttg tttatcagag atgtactggt tggtgaaaag
    21541 tacaacaaac attagtgaac gctactttat gcacaactgt atagtcgaat taaagtgata
    21601 aatgtgttga attgcattta taactagggt ggtaccgcga taacgttcgt cccttttgag
    21661 gatgggcgtt ttttattttt taataataaa aaatgatatt gatttatact agttttgcaa
    21721 atcgactatc tgtgtcatca atttttatgg aatggtaatt taaaaacagt aaataggatg
    21781 tgtaaatgat gaaaaagtta aaagcgagtg aaattagaca aaaatatcta gatttctttg
    21841 ttgaaaaagg acatatggtt gaaccttctg caccattagt gccaattgat gatgatacat
    21901 tattatggat taattcaggt gtagcaacat taaagaaata ttttgatgga cgtgaaacac
    21961 ctaaaaagcc aagaattgta aactctcaaa aagctattcg tacaaatgat attgaaaatg
    22021 ttggtttcac agcgcgtcac catacattct ttgaaatgtt aggtaacttc tcaattggtg
    22081 attattttaa acaagaagcg attgaatttg cttgggaatt tttaacgagt gataaatgga
    22141 tgggtatgga gccagataaa ttgtacgtta cgattcatcc ggaagatatg gaagcataca
    22201 acatttggca taaagatatt gggcttgaag aaagtcgtat tattcgcatt gaaggtaact
    22261 tctgggatat tggtgaaggg ccttcaggac cgaacactga gattttctat gatcgcggag
    22321 aagcatatgg acaagacgat ccggcagaag aaatgtatcc aggtggagaa aatgaacgct
    22381 atcttgaagt atggaactta gtatttagtg aattcaatca taataaagat catagttaca
    22441 caccattacc taataaaaat attgatactg gcatggggct tgagcgtatg gcctcagttt
    22501 ctcaaaatgt acgtactaac tatgaaacag atttatttat gcctataatg aatgaaatcg
    22561 aaaaagtatc aggtaaacaa tatttagtaa acaacgaaca agatgtggca tttaaagtaa
    22621 ttgctgacca cattcgtacg attgcatttg caatttctga tggtgcatta cctgccaatg
    22681 aaggtagagg gtatgtatta cgtcgattgt tacgtcgtgc cgttcgtttt agtcaaacgt
    22741 taggaatcaa tgagccattt atgtacaaac ttgttgatat tgttgcagac attatggaac
    22801 catattatcc aaatgttaag gaaaaagcag atttcattaa gcgtgttata aagtctgaag
    22861 aagaacgatt ccatgaaaca ttagaagatg gtttagcgat tttaaatgaa ttaattaaaa
    22921 aagctaaagc gacaacaaat gaaattaatg ggaaagatgc atttaaattg tatgatacgt
    22981 atgggttccc aattgaatta actgaagaaa tagcagtgca agcaggattg aaagttgata
    23041 tgacaacatt cgagtcagaa atgcaacaac aacgtgatcg tgcacgtcaa gcacgtcaaa
    23101 attctcaatc aatgcaagtt caaagtgaag tattgaaaaa tattacatct gcaagtactt
    23161 ttgttggtta tgatactgcg acagctcaaa caacactaac acacttgata tacaatggtg
    23221 aagaagtttc acaagttgaa gcgggtgaaa cagtatactt catgttaacg gaaacaccat
    23281 tttatgcaat cagtggtgga caagttgcgg atacaggtat tgtttataat gacaattttg
    23341 aaattgctgt tagtgaagta accaaagcac caaatggtca aaacttgcat aaaggagtag
    23401 tacaatttgg ccaagtaaat gttggcgcta cagtgtctgc tgaagtgaac caaaatgatc
    23461 gacgtgacat tcaaaagaac catagtgcaa cacatttatt acatgcagcg ttgaaatcag
    23521 tactgggtga tcatgttaac caagctggtt cactagtaga agcagatcgt ttacgttttg
    23581 atttctctca ttttggtcca atgactaatg atgaaattga tcaagttgaa cgcttagtaa
    23641 atgaagaaat ttggaaaggt attgacgtta acattcaaga aatggatatt gcttcagcta
    23701 aagaaatggg cgcaatggca ttattcggtg aaaaatatgg tgatgttgtg cgtgtagtaa
    23761 atatggcacc attttcaatt gaattatgtg gtggtattca tgtccgcaat acttctgaaa
    23821 ttggcttatt caaaatagta agtgagtcag gtacaggagc tggtgtgcgt cgtattgaag
    23881 cattaacagg taaagcagct ttcttatatt tagaagatat tcaagagaaa tttaatacga
    23941 tgaaatcaca gctgaaagtg aaatctgatg atcaagtagt cgataagtta acacaattac
    24001 aagatgaaga aaaagcatta ttaaaacaat tagagcaacg tgacaaagaa atcacatcac
    24061 ttaaaatggg taatattgaa gatcaagttg aagaaatcaa tggctataaa gtattggtta
    24121 ctgaagtgga tgtaccaaat gcgaaagcaa ttcgctcgac aatggacgat tttaaatcta
    24181 aactacaaga tacaattatc attcttgcaa gtaatgttga tgataaagta tcgatggttg
    24241 caactgtccc taaatcttta acaaataacg ttaaagccgg tgatcttatc aaacaaatgg
    24301 caccaatcgt tggtggtaaa ggtggcggtc gtccagatat ggctcaaggt ggcggtacac
    24361 aacctgaaaa tatctcaaaa tcattaagct ttattaaaga ttacattaaa aatctataat
    24421 ttaaagccat aatattgtat gatgatagtg agttatattt gtaattaaag gagtgtcgca
    24481 aatggaaaac tttgataaaa caatgaaatt cgactatgaa gaacttccaa cgcaagatgt
    24541 aagagatgtt ttaaataatg tttatcgaac attagatgaa cgaggatata atgccgtaaa
    24601 ccaaattgta ggttatttat tatcaggtga ccctgcgtat attccacgcc aaaatgaagc
    24661 aagaaatcaa attcgtcata ttgatagaga tgttattatg gaagagcttg tttcttacta
    24721 tttaaaagag caaaataaat aatttatgtt acaacataaa attttaggac tcgatgtcgg
    24781 tagtagaacg gtaggaattg caattagtga tataatgggt tggacggcac aaggattaga
    24841 cacactccga atcaatgaag aaaataatga attaggtatt gaccaattag tagacattat
    24901 taaaaaacat aatgtgggta cagtcgtaat aggactacct aaaaacatga ataattcaat
    24961 aggatttcga ggcgaagctt cgttaacata taaagaaaaa ttattagaag cttatccttc
    25021 tattgagatt gtgatgtggg atgaaagatt aagcacaatg gctgctgaac gatcattatt
    25081 agaagcagat gtttcaagac aaaaaagaaa acaagtgatt gataaaatgg cagcggtatt
    25141 tattttacaa ggctatttag attcactaca ttaaaaggag attttactat gactgaacat
    25201 aatcatgatt cacaactaga aattaataac gaagaagaat tattaacttt attcgatgaa
    25261 gagggaaatg aagttttata ccgaaaagtt ttagaatttt atcatcctga attcaaaaaa
    25321 gagtatgtta tcttagctga agaaggtgct caatcagatg aagacgatat gattgagctt
    25381 gtaccaatga tcaatgaacc agatgagtca ggtgacggtg gtaagttagt accaatcgaa
    25441 actgatgaag aatgggacat gattgaagaa gttgtaaata ctgaaatgga agaataatta
    25501 aaaagatttt actcttgcta accttaaaga tggggaggca agagtatttt ttaagtcaag
    25561 caatgtataa ataattaacg cttataattt tattgaaaat atggtaaaat atctaaagtt
    25621 aaattttata tgaatgattc gcttctataa ataaaatagc tttgcgtcta acacgaaatc
    25681 agtcatttaa ttggttggca aaattataat caattgtgaa ggctgattta ttttattgtc
    25741 ataatcatat tgacttattt tacataaagg aagtttaaaa catggatgac ctaaataaaa
    25801 aatatttaat agatttacat caacatcaaa atagttcaat cgaagttttg cgtgaatttg
    25861 ccgaggtaaa tgaagtgcca attgtagatc gtttaacatt agatttaatt aagcaattaa
    25921 ttcgtatgaa taatgttaaa aatattttag aaattggtac agcaatcggc tatagttcta
    25981 tgcaattcgc ttctatatct gatgacattc atgtcacaac gatagagcgt aatgaaacga
    26041 tgattcaata tgctaaacaa aatttagcta cttatcattt tgaaaaccag gttcgaatta
    26101 ttgaaggtaa tgctttagag caatttgaaa atgtaaatga caaagtttat gatatgatat
    26161 ttattgatgc agcaaaagcg caatcaaaga aattttttga aatatataca ccacttttaa
    26221 agcaccaagg tctcgtaatt acagataatg ttttatatca cggttttgta tcggatattg
    26281 ggattgttag atcgagaaat gtaagacaaa tggttaaaaa ggttcaagat tataatgagt
    26341 ggttaataaa gcaaccagga tatacaacga attttttaaa tatagacgat ggattagcga
    26401 tttcaattaa aggagaatga ctatgacaga attacttgtc acacccaagt cattaagcca
    26461 tatggaaaca cttattgatt taggtgcaga tgcttttgta attggcgagc aaaagtttgg
    26521 tttaagactt ccaggggaat tcaatagaca acaaatgaca gaggctgtcg cattagctca
    26581 caaaaacgat aaaaaagtat atgcggcagt gaatggatta tttcataatt atcatttaga
    26641 tgctgtagaa gattatatta attttttaca tgaaatacga gttgacagaa ttattttcgg
    26701 ggatccagca gtagtaatgt atgtgaaggc gcaagaaaat ccaataccac ttcattggaa
    26761 tgcagagacg ttagtaacta atcattttca atgtaattac tggggtaaaa gaggcgcatc
    26821 aagagctgtc ttagcacgtg agctgaattt ggatgaaatt attaatataa aagaaaattc
    26881 aaatgtggaa attgaggtgc aagttcaagg aatgacttgt atgtttcaat caaagcgtat
    26941 gctactaggc aattattata cgttccaaga tcgccaaatg aaaattgaac gtcgcaatga
    27001 tgaacaatct ttattacttt atgatgaaga aagacaaaat aattacccag tttacgaaga
    27061 ttacaatggt acgcacatta tgtcaccaaa cgacatttgt ttaattgaag aattagcacc
    27121 attttttgag gcgggtatag attcatttaa aatcgatggt attctacaaa cggaagaata
    27181 tattaatgtg gtaacggaac agtatcgtca agcgatagat ttgtacaatg aagatcctga
    27241 aatctatgaa gatgagaaat ttatgttgat ggatccaatt gaagaaattc aacctgatca
    27301 tcgaccattt gacgaaggtt tcttatataa acaaacagta tattaaggag gttaatcatg
    27361 aagacaatag aagagattaa atcaactcct aaaacagtta tgaagaaacc agaattatta
    27421 gcacctgctg gaaacttaga aaagttaaaa atagcagtac attatggcgc tgatgccgta
    27481 tttttaggtg gtcaagaata tggattacgt tcaaatgctg ataatttcac gatggaagaa
    27541 atagctgaag gtgttgaatt tgcgaaccgt tacggtgcca aaatttatgt tacgacaaat
    27601 attattgcac atgatgagaa tattgaaggt ctagaatcat atttgcgtaa tttggaaaag
    27661 actggtgcga caggtatcat tgttgcagat cctttaatta tcgaaacatg taaagaagtt
    27721 gcgccaaaac ttgaaattca tttatctact caacaatcac tttctaatta caaagctgta
    27781 gaatattgga aagaagaagg attggatcgt gttgtattag cacgtgagac cggcgcgatg
    27841 gaaatgcgtg aaatgaagga aaaagtagat attgaaatcg aagcatttat tcatggtgct
    27901 atgtgtatcg cctattcagg tagatgtaca ttaagtaatc atatgactgc aagggattcc
    27961 aacagaggcg gttgctgtca aagttgccgt tgggattatg aattattaga agttgatgat
    28021 aatggtgaac ttgatgtttt ttataatcaa ggtgaagtta caccgtttgc gatgagtcct
    28081 aaagatttaa aattaatcga atcaattcct caaatgatgg atattggtgt ggactcatta
    28141 aaaattgaag gacgtatgaa gtcaattcat tatattgcaa cagttgtctc agtatatcgt
    28201 aaagtcattg atgcgtatgc ggcagatcct gacaacttta agattaatcc ggaatggtta
    28261 atagagttag ataaatgtgc aaatagagac actgcaccag cattctttga gggaacacct
    28321 ggttatgaag aacagatgtt tggtcaacaa caatctaaaa aatcaccatt tgatttttgt
    28381 ggtttggtat tagactataa tgaagataca aaaattgcga ctattcaaca acgaaataac
    28441 tttaaaccag gtcaagaaat agaattcttt ggtccagaaa ttgaaacatt tacacaagta
    28501 gtagaagcaa tttatgatga agaaggtaac agcttagacg cggcgcgcca tccattacaa
    28561 atcgttcaaa ttaaagtgga tcgcccgata tatccaaaca acatgatgag aaaggaaatt
    28621 ggctaatgaa agctactaca atcattggca tagctggtgg atctggctca ggaaaaacaa
    28681 ctgtaactaa cgaaattatg aaaaacttag aaggtcatag tgtcgcttta cttgctcaag
    28741 attactatta taaagatcaa aagcacttga ctttcgacga gcgcctagaa accaattatg
    28801 accatccatt tgcattcgat aatgatttat taattgaaaa tcttaaagac ttgaaaaatg
    28861 gtaaagcagt agaagtaccg acatatgatt atgctagtca tacaagaagt gacattacca
    28921 ttgattttaa acctaaagat gttattatcg tagaaggcat tttcgcttta gaaaataagg
    28981 tattacgtga tatgatggat gttaaaatat atgttgatac agatgcagac ttgagaatat
    29041 tacgccgttt aacacgagat actaaagagc gtgggcgttc aatggactct gttatcaatc
    29101 aatatttaag tgttgttaga cctatgcatg accaatttat tgaaccgact aagaaatatg
    29161 ctgatataat tattcctgaa ggtgggagca ataaagttgc aatagatatt atgacaacaa
    29221 aaattcagtc tttagttagc aagcaatagt ataattcagt aaaggaagat gacaatatgg
    29281 aaaatcaaaa gcaatatcca atgactcaag aaggttttga aaaattagag cgtgaacttg
    29341 aagaattaaa aacagttaag cgtcctgaag ttgtagagaa aattaaagtt gcacgttcat
    29401 ttggtgactt atcagagaac tctgagtatg atgcagcaaa agatgaacaa ggattcatcg
    29461 aacaagatat tcaaagaatt gagcatatgt taagaaatgc attaatcatt gaagatactg
    29521 gagataacaa cgttgttaaa attggtaaaa cagtaacgtt tgtagaatta ccaggtgatg
    29581 aagaggaaag ttatcaaatc gttggttcag ctgaatcaga tgcatttaat ggtaagattt
    29641 caaatgaatc accaatggct aaagcgttaa ttggtaaagg tttagatgat gaagttcgtg
    29701 ttccactacc taatggtggc gaaatgaacg taaaaattgt taatatccaa taaatgatca
    29761 aatagattat ttaattgcgc gattgaagtg tgttaatgat acttcggtcg tgctttttta
    29821 tattcaaaat caaaaaatcg gacagatgaa aagtaaaaat tttccatcag accgaatgat
    29881 agcagaacct aaattagtgt aagagttaca atattaacta ctccatttta aaaaagcaaa
    29941 atatttccga gaaaacaaat tgattatcat ttgttattgt atgcgatttg aattacttat
    30001 taaacaactt gtgcttctca atgatacaat gaaaaggtat tcaatactgg tttattgcaa
    30061 tacaaagggg gatttaaagt ggatgtaaga tttattaacg agcagacaat tatgatttat
    30121 tttgaaaata aaatatccga agagacatat cgtaatgtca cagcaatggt acggtggata
    30181 cgagaaaaag aaatattaga aattcaagat attgtccctt cgtatcgagc agttctaata
    30241 tattttgatg aacaagctat aacatcttct aaattaattg aaaatttaga acttaacaaa
    30301 tttaatgaaa aaaatgttca tgctgtgaat caaaccaatc gtattattaa aatacctgtt
    30361 caatatggtg gtacatatgg accagatatt gaagaagtag caaagcataa tcgaataact
    30421 gttgagcaag ttattgaaaa acatacaagt aaaccttatt taatatatat gctaggattt
    30481 atgccaggat ttccatactt aggcggactt gatgaacaac tacatacacc tagacgtaat
    30541 cagccaaggt taaaaattca tgcaggttct gtaggtattg cgaataatca aacaggacta
    30601 tatccatcag attctcctgg cggatggcaa attattggac gcacaccgtt aaaagtattt
    30661 agcagcgagc gtgaaccaat gtcaatgtac gaagcaggag aatggataca attttatgca
    30721 atagatgaac agaaatttat acaaatcgaa agagatatat cagatggcaa ctttaatgtt
    30781 gatgactggg tggtgattga aaatgtcaat taagatttta caaccaggtc tcttttcaac
    30841 ggtacaagat ctaggaagaa taggttatga acatattgga ttttcaggcg caggtgccat
    30901 ggatcaattt agttttaagg ttgcgcagtc attaattaac aatgatggtc cagcgattga
    30961 atatactttg attggtccta ccattcaatt taatacgcaa aatacatttg ttataaccgg
    31021 tggtagtatt aatgcttcgc taaataataa aactatatca atgaattctg tcatattagc
    31081 tgagaaaggt gacattttaa aaataggtgc tataaccaaa ggtgcacgcg gttatcttac
    31141 ttttggtcat tctatcaacg taccttctat tgctgaaagt tatgcaacac atacccgaag
    31201 cagtatagga ggattcaaag gtagaaagtt attagctgat gatgtaataa cagtgaaaat
    31261 aaataatgac tttaaagaaa acattggaaa gacgattcat ttacaggacg atttattgcc
    31321 agaaaataat attatacaca ttcttcaagg acctcaattc gaggcatttt ctgaagaggc
    31381 tagagcgaaa attgtaaatc atccatattt aattactgaa caatcagacc gtatgggcta
    31441 tcgtttggaa ggtgacagcg ttgcaccaat taatcaagca gatatcattt ctgaaccggt
    31501 tgctcttggt agtgttcagg taccaaatga tggtcaacct attattctgc ttaatgataa
    31561 acaaacgata ggtggttata ccaaaattgc aactgtatgc aaatttgatc tgccaaagtt
    31621 agcacagatg aaaccacaag atacaattca atttaaatgg atatcattcc aagaagctgt
    31681 agataagaat cgggaacaaa tgtctctgtt taatgaaatt cttaagagtc atcaaaagac
    31741 tccaattttt gatacatcta gcttgcgaca tacttcgaaa aaattagcaa caattttaaa
    31801 aggggatttg taaaatgaat attgaaaaaa tcgaacaaat aatcaaatta gtgaaggaaa
    31861 atgatgttaa gaaatttaaa tataaaaatt ttgaagatga aatagaaatt gacttcactg
    31921 actcgaatca tttggctgca cacagtaatc aatcaaatca aagtatgaac aataatgatt
    31981 tgacagcttc aaaagcgaat gataactccg atgtttcgac aaatgattat catgacatta
    32041 aatcaccaat gataggtaca ttctttttac aagatagtaa agaattaact gaaccaattg
    32101 tgaatgtcgg tgacaaagtt aacaagggag atattatagg atatgttgaa gcgatgaaag
    32161 tattaaacga ggtaacaaca gatgttgctg gagaaattac tgaaatagta gctgatcatg
    32221 gcacaaatgt tgaatacgac caagttttgg tacgtattaa gtaaaggggg taagtaaatg
    32281 cttcgttgtt taattgcgaa cagaggtgaa attgctgtaa ggattataag agcttgcaga
    32341 gaatatggga ttgaaactgt agcagtttat gcaaaagggg acgagcaaag cttgcatgta
    32401 catttagcag atcaagctat atgtattggg gaagctaatg ctttagatag ttatttaaat
    32461 attgaccgca tcatatctgc tgcacaaatc actggtgcta atgcaattca cccaggatat
    32521 ggctttttat cagaatcaac aaaatttgct caaaccgttg aggaacaagg catagcgttt
    32581 atagggccta ctaaaaaaac gatggaaatg atgggggata aaataacagc aagacaaaca
    32641 gttcatcatg caggtgttcc tgtgattcca ggatcaaatg gtgctgtgaa tcatgtgtct
    32701 gaaattgaga atcttgccaa agacatcggt tatccagttg ttatcaaagc tgccagtggc
    32761 ggtggtggta aaggtatacg tattgtaaag aaagctgaag atttagaaaa ggcatttaaa
    32821 gaagctaaaa gtgaaggaaa aaaatacttt gatgatgatc gtgtttatgt tgaggctttt
    32881 atacctgttg caaaacatgt tgaagttcaa gttatgggag atgggcagga taattatgta
    32941 catttagggg aacgtgattg ttctgtacaa cgtaaaaatc aaaaattaat cgaagaatct
    33001 ccttgtgcag cattaactga agaaagacga caacaaatat gtaacgatgc tgttaaagtt
    33061 gcacgtgcag caaattatag aagtgcaggt acgattgaat ttttagtaac tgataccgca
    33121 cattacttta ttgaaatgaa tgctcgaata caagttgaac atactgttac agagatgcga
    33181 gcagaacgtg atttggttgc agcacaactg tatttattgg aacataatca tttacctttt
    33241 tcacaaagcg atattcaatt taatggtcat gtgatagaag cgagaataaa tgccgaaaat
    33301 cctgaaaaga aatttcaacc aacaccaggt aaagtgacag cgttacattt acctcaaggc
    33361 tttaatgtgc gagtcgattc tttactatat cacggttatc aagtttcacc atattatgat
    33421 tctttagtag ctaaagtaat tgtaaaatct catgatagag cttcagcaat tgataagttg
    33481 aaagtcacct tagatgaaat ggtgatagat ggattttcta caactgccga ttttttatat
    33541 gcagttttaa attatccact atatcgtgat ggagatgcaa aagatgttga tattaaattt
    33601 ttagaaaaac atcaaattgt taaaggggtg gaattataat gcgagttgat ttgaattgtg
    33661 atttaggcga agcatttgga aattattcct ttggtggtga tcatcaaatt attccgttaa
    33721 ttacaagtgc gaatgttgct tgtggttttc acgctggtga tgaaaatgta atgaatgaaa
    33781 cggtaaaact tgccaaagca cataatgttg cagtaggtgc acatcctggt ttacctgatt
    33841 tgaaaggctt tggcagacga aatatagata tctctaacga cgagatttat aatttgatga
    33901 tttatcaatt aggtgcatta caagggtttt gtcgcattca tcaagttaaa attaatcatg
    33961 ttaaaccgca tggtgcattg tatcagatgg gtgcaaaaga cagagaaata gcaaacgtta
    34021 tagcacaagc tgtttatgac tttgatccat cactagtgtt agtaggatta gcaaattcat
    34081 atctaatttc agaagcaaag aatgtcggat taattacagc ttctgaagtg tttgctgata
    34141 gacgatacga agatgatggg cagctcgtta gtagaaaaga aagtgatgct gtgattactg
    34201 atactgacga agcacttaag caggttttaa agatggtgaa ggaaaataaa gttatttcaa
    34261 aaaacaataa ggaagtaacg ttacaagcag atacaatttg tgtgcatggt gatggagaac
    34321 atgcattatt atttgtttcg aaaattagag aaattttaat gaaagaaggc attgatattc
    34381 aatccttata gggggctaaa aaaatgggga gaaatttaaa gcttaaaaaa gaaagtgact
    34441 ttgaattcac aaaaaatcat aaaaggttat tattaggttc tgtatttttg atggcaactt
    34501 ctgcaattgg gccagcattt ttaacgcaaa cagcagtatt tacatcacaa ttttttgcaa
    34561 gtttcgcatt tgccatatta ctgtctatca tcattgacat tggtgcacaa attaatatat
    34621 ggcgcatatt agttgtaact ggtttaagag gtcaagaaat atcaaataaa gttgttcctg
    34681 ggcttggtac tgttatctca atattgattg catttggtgg tctagctttt aatattggta
    34741 atattgctgg tgccggttta ggtttaaatg caatttttgg attagatgta aaatggggcg
    34801 cagctattac tgcaatcttt gcaatattaa tctttgtaag taaaagtggc caaaaaatta
    34861 tggacgttgt ttcaatgatt cttggtattg tgatgatttt agttgtggca tatgtgatgt
    34921 ttgtttctaa tccaccttat ggtgatgctt ttgtgcatac atttgcgcca gaacatccaa
    34981 tgaaattagt cttgcccatc attacgttag ttggtggaac tgtaggtggt tatattacct
    35041 ttgcaggtgc acatcgtata ttagactctg gcattaaagg taagcaatat ttaccatttg
    35101 taaatcaatc agcaattgct ggtattttaa ctacaggtat tatgagaacg ttactattcc
    35161 tagcggtatt aggagttgtt gtaacaggtg tgacactaag ttctgaaaat ccaccagcgt
    35221 cagtttttga acacgcaatt ggaccaattg gaaagaatat ttttggtatt gtgttatttg
    35281 ctgcagctat gtcatcagta attggctcag catacacaag cgcaacattt ttaaaaacac
    35341 ttcataaatc acttaacgaa agaagtaatt taattgtgat tgtgtttatc gttatttcaa
    35401 caatgatttt cttatttatt ggaaaaccaa tcagcctttt aattatagca ggcgcgataa
    35461 atggatggat tttacctatt acgttaggtg caattttgat tgcaagtaaa aagaaatcaa
    35521 ttgttggtga ttataaacac ccaaattgga tgttcatctt tggtatcgtt gcagtacttg
    35581 ttacaatatt gacaggtata ttctcattca aagaagtact tcagttattt taaaagacat
    35641 ttcgactaat agcatcactg taaatgttgt tagtattagg caagatatat tttgatttcc
    35701 ggtgattcat ttataaagca taattagata tattttaagg gagagtggtg catgacaaat
    35761 gtactgctct ttttgttgtg tatcagtatt gtgatgtttt aatgagaata ttatatgaat
    35821 cattatgaaa tttaataaaa ataaaagaaa tgattatcat tttttcttat atactgttaa
    35881 acggtttgga atttttaggt atacactgta ttggttgata taactcaact aataattgcg
    35941 aacagagtat ttcaaattga aaagtattat gagcgtgata cataatcaaa attgtaggct
    36001 caagaaccac tacataataa accataagcg gttctttatc atttatgtct cgctctcaaa
    36061 tgtaaattaa taattgtttt gggggagttt gaagttaaat atttaacagg atttatttta
    36121 atattattgt tagaaggaat ttttacaaat tcagcgagtg caatcgaata ttcagactta
    36181 catcataaaa gtaagtttga ttcaaagcgt ctaagtaatg ctaagatgtc attcatcaat
    36241 ccaactcagc ttgaaaataa aaacacaaac gatagactgt tgaagcatga tttgttattt
    36301 catgacatgt tcgtaaatga tgattggaaa aaggatttta aagttgaatt tgaaaatgag
    36361 gcactttcaa agaaatttat aaataaggat atcgatatat ttgctggaaa ttatggatac
    36421 ggatgtcatg ggggagcaac caataaaacg caatgtagtt atggtggtgt tactttaagt
    36481 gacaataata aatacgatga ttataagaat ataccttgta atttatggat tgacggacat
    36541 caaacagaaa tagaactaac tgcagtaaaa acgaaaaaga aaattgttac tattcaagaa
    36601 ttagaggttc aattaagaaa ttatttgaat gagaagtata agttgtacga acaaggtggc
    36661 gacattgtta aagggtatgt taaatattat aatgatgatg aacaaaatgt agaatatgat
    36721 ttttataatt taaatggtga gtatggtcgt gaggtattaa aaatgtatgc tgataataaa
    36781 actatcaata gtgacaaatt gcatttagat atctatttat tcaaatcata atttaattaa
    36841 gttaatcatt aataatcttt gacagtgaaa tataaagtac ctgtcattat gttgttacaa
    36901 ataaaaacag cagtaaggat gctaacactt agaaaagtct tactgctgtt ttctattaga
    36961 attatcttta atgcacaaag gaattcgata cgaaacaaat atgattaact gtgtaattac
    37021 aaaataaaac tactatagta aaaagaagat aaagacgatg ataataaatg catcgaatat
    37081 aattttcttg atgtttttat caacaacatc aggtaaggac ataagtataa atcctagaat
    37141 tagagtgtag cgacatatgt tcataattgt tgctgtaaac tcgatattaa atgaaaactt
    37201 taaaaatata cttataaata aaatgacaaa cgacgcaatt gcaataaaac tgccgtatgt
    37261 agctatcttt gttacgagac tattttgatt attttcagcc attgtaaaat cctcttattt
    37321 catcattatt attttaaaca tatcattttc ctgttattta ataaagtttc ttgacacgat
    37381 tggttgatgt aattgatatg attagttttt gaaaaataga caaattagaa gcatcgaatt
    37441 cactgttatg agttcaaaaa taattattta tgataccaag ttattgaaag agctttttat
    37501 aaaaataaag caaaagtttt atacagtaat gaaaatcatg atacttgtcg ttgtagtgat
    37561 taaaatgtga taaaataaca aatgctagga ttttttaaaa gaggtgaatc aaaagatgat
    37621 tggtataatt ggtgccatgg aagaagaagt aacaatatta aaaaataaat taacacaatt
    37681 aagcgaaatt tcagttgcac atgttaaatt ttatactggc attttaaaag atagagaagt
    37741 agtgattacc caaagtggca ttggaaaagt taatgctgca atttctacga cattattaat
    37801 taataagttt aaaccggacg tcattattaa tacaggttct gctggagctt tagatgaaag
    37861 tttaaatgta ggtgacgttc ttataagtga tgatgtaaaa tatcatgatg cagacgcaac
    37921 agcatttggt tatgaatatg gacaaatacc acagatgccg gtagcatttc aatcaagtaa
    37981 acctttaata gaaaaagtat ctcaagttgt acaacaacaa caattaacag ctaaagtagg
    38041 cttaattgta agtggtgata gctttatcgg tagtgttgaa caacgccaaa aaattaaaaa
    38101 agcatttcca aatgcgatgg cggttgaaat ggaagcaact gcaattgcac aaacatgtta
    38161 tcaatttaat gtaccatttg ttgtagttcg tgcagtttca gacttagcaa atggagaagc
    38221 ggaaatgagc ttcgaagcat ttttagaaaa agcagctgta tcatcaagtc aaactgttga
    38281 agcattagtg tctcaattat aaaaaggtaa aggtgagtta aatgggttta gttcgcaagt
    38341 tttttatgcc gaattcatat gttcaatcaa tatttcaaat tgatttagac aagttagtgg
    38401 acaaaggcgt taaaggtatt attacagatt tagataatac gctagtaggt tgggatgtta
    38461 aagaacctac agaacgtgtt aaagcatggt ttaaggaagc taatgaaaaa ggaatcacta
    38521 ttacaatcgt gtctaataat aatgagtctc gtgttgctag ttttagtcag catttagaca
    38581 tcgattttat ttttaaagcg agaaagccaa tggggaaagc gtttgataaa gcaataacta
    38641 agatgaatat cagaccagat caaactgttg ttataggtga ccaaatgctt actgatgtat
    38701 ttggtggtaa tcgtcgaggt ctatatacaa ttatggttgt tccagttaaa cgaactgatg
    38761 gctttattac taagtttaat agattaattg aaaggcgatt attacgtcat ttcagtaaaa
    38821 aaggttatat cacatgggag gaaaattgat tgtctgacat tttaaaatgt atcggttgtg
    38881 gtgcgccact tcaatctgaa gataaaaata aacctggttt tgtaccagag cataatatgt
    38941 ttcgtgatga cgtgatttgc agacgttgtt tccgcttgaa aaattataac gaagttcaag
    39001 atgtaggatt agaaagtgaa gactttttaa aattattatc aggacttgcg gataaaaagg
    39061 gtattgtcgt caatgtcgtg gatgtatttg actttgaagg atcatttatt aatgcagtta
    39121 aacgtattgt cggaaacaaa aaaatcattt tagcagcaaa taaattggat ttattgccga
    39181 aacaaattaa taaacgtcga gttaaagaat ggttaaaacg aacagcaaga aaatatggtt
    39241 tggaagctga cgatgtcgta ttaatttcag ctgaaaaagg ctggggcata gacgacttat
    39301 tatcatcaat tgcgaatatt cgagaaaatg aagatgtgta tattgtaggg acaacgaatg
    39361 ttgggaaatc tacattgatt aataaactga ttgaagctag tgttggtgaa aaagatgtag
    39421 taacaacttc aagattccct ggaacaactt tagatatgat agatattcct ttagatgaaa
    39481 catcatttat gtatgataca ccaggtatta ttcaagatca ccaaatgacg catttagtta
    39541 gtgaaaaaga attgaaaatt attatgccta agaaagaaat aaaacaacgc gtatatcaat
    39601 taaatgaggc gcagacatta ttcttcggcg gtctagcgcg catagattat gtatcaggtg
    39661 gtaaacgtcc gttagtttgt ttcttttcta atgacttgaa tatacatcgt actaaaacgg
    39721 agaaggctaa tgatttatgg cgtaatcaac ttggcgattt attaacgcca cctggaaatc
    39781 cacaaaattt tgatcttaat gaggtaaagg ctgttagact tgaaacaggc aaagagaaac
    39841 gcgatgttat gatctctggt ctaggcttta taactatagg accaggggct aaagtaatcg
    39901 ttcgtgttcc taaaaatgtt gatgttgtat taagaaattc tattttataa ggtgattaaa
    39961 aaaatgaaat ttgcagttat aggaaatcct atttcacatt ccttgtcgcc cgttatgcat
    40021 agagcaaatt ttaattcttt aggattagat gatacttatg aagctttaaa tattccaatt
    40081 gaagattttc atttaattaa agaaattatt tcgaaaaaag aattagaagg ctttaatatc
    40141 acaattcctc ataaagaacg tatcataccg tatttagatt atgttgatga acaagcgatt
    40201 aatgcaggtg cagttaacac tgttttgata aaagatggca agtggatagg gtataataca
    40261 gatggtattg gttatgttaa aggattgcac agcgtttatc cagatttaga aaatgcatac
    40321 attttaattt tgggcgcagg tggtgcaagt aaaggtattg cttatgaatt agcaaaattt
    40381 gtaaagccca aattaactgt tgcgaataga acgatggctc gttttgaatc ttggaattta
    40441 aatataaacc aaatttcatt agcagatgct gaaaagtatt tagctgaatt cgatatcgtt
    40501 attaatacaa caccagcggg tatggctgga aataacgaaa gtattattaa tttaaagcat
    40561 ctttctccca atactttaat gagtgatatt gtttatatac cgtataaaac acctatttta
    40621 gaggaagcag agcgcaaggg aaaccatatt tataatggct tagatatgtt tgtttaccag
    40681 ggtgcggaaa gctttaaaat ttggactaat aaagatgctg atattaattc tatgaaaaca
    40741 gcagttttac aacaattaaa aggagaataa catatgctta ctggcaaaca aaaaagatac
    40801 ttaagaagtt tagcacacaa tattgatccg atttttcaaa ttggaaaagg cggtatcaac
    40861 gaaaatatga ttaaacaaat agatgatacg ttagaaaaca gagaattgat taaagtacat
    40921 gtactacaaa ataactttga tgataaaaaa gaattagctg aaacattaag cgaagctact
    40981 catagtgaat tagtgcaagt gattggatct atgatagtga tttatagaga atctaaagat
    41041 aataaagaaa ttgaattgcc ataataatga aaaagatagt actttacggc ggtcagttta
    41101 accctatcca tactgcacat atgatagtag ctagcgaagt atttcatgaa ttacagccag
    41161 atgaatttta ttttttacct agttttatgt ctccattgaa aaagcaccat gattttatag
    41221 acgttcagca cagattaaca atgatacaga tgattatcga cgagcttggt tttggagata
    41281 tttgtgacga tgaaattaaa cgtggtggtc aaagttatac ctatgacacg atcaaggcat
    41341 tcaaggagca acacaaagac agtgagttgt actttgttat tgggacggat cagtataacc
    41401 aactagagaa atggtatcaa attgaatact taaaagaaat ggttactttt gtagttgtaa
    41461 atcgagacaa aaatagtcaa aatgttgaaa atgctatgat tgcaattcag atacctaggg
    41521 tagatataag ttcgacaatg attcgacaaa gagttagtga agggaaatct atccaagttc
    41581 ttgttcctaa atccgttgaa aactatatta agggggaagg attatatgaa cattgaaaaa
    41641 gcaaaacggc ttgcaaaaga gaaactgcca gagaaacgtt ataatcattc tttaagagtt
    41701 gcagaaacag cgattaaatt ggcagaaatt tatgatggag acactagtaa agtagaatta
    41761 gcaggtgtat tacatgattt ctgtaaatat gatgatttag gtaaaatgta tcaaattgtt
    41821 cgacaatacg aattaggtaa tgatctactg agttatggta gtgaaatatt gcatggccct
    41881 gtgtgtgcag caatcatgga acatgaatat ggtatcaatg atgaggaagt attaatggct
    41941 atcaaatacc atactactgg acgtcaacaa atgacaaaaa ctgaaaaact gatttttatt
    42001 gcagattaca tcgaacctgg aagaacaatc ccaggagttg atgatattcg agatatggca
    42061 tacaatcaag gtagtttaga taagacaatt tatgaaattt ctaaacgcac agtactattt
    42121 ttaatacaga aagatattac ggtatataat aagacgattg actgtttaaa ttattataac
    42181 tatagtgacg aaagaataaa ggatgattaa atgaattcac aagaattatt agcaattgct
    42241 gtggatgcaa ttgacaataa aaaaggcgaa gatacgattt ctttagaaat gaaaggtatc
    42301 agcgatatga cagattattt tgttgtaacg cacggaaata atgaacgaca agttcaagcg
    42361 attgctagag cggtgaaaga agtagccaat gaacaaaata tagaagtaaa acgtatggaa
    42421 ggatacaatg aagcgcgttg gatattaatt gacttagctg atgttgtggt acatgttttc
    42481 cataaagacg aaagaaatta ttataatatt gaaaagttat atcaagatgc accattagaa
    42541 tcatatagtc aggttgcgta ttaattatgt cgcaatatgc agaaatgagc ctagtgtacg
    42601 atcaattgac tcaagatcaa ccatatgaaa aatggtttga aattgtaaaa aatcactgca
    42661 aagatgaatc aaatatttta gatattggat gcggtactgg tagtttaaca gttcaattag
    42721 aagctttagg taatgttact ggtatggatt taagtgttga tatgttgact gttgcagctc
    42781 aaaaatcagc aaatgtaaat tggcttgaag gagatatgac atcttttgat ttgcaacaac
    42841 aatttgatat catcactatt ttttgtgact ctctaaatta tttgcaagat gaaactgcag
    42901 tgattgaaac attcatcaat gtttatcatc atctgactga ttcaggtgta tttatttttg
    42961 atgtacatac tgtttataaa atgatgactt tgtttaataa tcaaagttat attgatgata
    43021 aaggggacat ttttttagca tgggatgctg tgcaaggaga tttaccttta agtgtttatc
    43081 atgatatgac atttttcatt cgacatgaag atgaaacata ttcaagattt gatgagtctc
    43141 attttcagag aacatttgat gaaaagacat atttgtcttg gttagctcaa gttggtttta
    43201 aacatgttga aacatttact gattttaata tagatgaaca taatgaagat gcagaaagat
    43261 tgtttttcat tgcgaaaaaa taaaagtaaa accactcgct cagacgtgta taaagttgag
    43321 gagtggtttt attgtatcaa tttttattac gctataaaga ttttttaact caatggaagt
    43381 tatatattct aagtgctgtt gttttaatta tggtattaat tggttttata ttctggagac
    43441 aagatgatta tacttcaaga aattttgaaa ataaagatac tgctctgaaa caaagcacta
    43501 gtgaaaatag tagtttgtcc aaagtagaag atgtccaggt caaagatgga gataattcca
    43561 aaaataaggg tcctgtatat gtcgatgtaa aaggtgctgt taaacatcct aatgtttata
    43621 aaatgacatc taaggataga gtagttgatt tacttgataa agcacaatta ttggaggatg
    43681 cagatgtaag tcaaattaat ttgtctgaaa aattaacaga tcaaaaaatg attttcatac
    43741 ctcataaagg acaaaagaat gttgaaccac aaattgaagt aaacagtgtg cacgaaaaaa
    43801 atgggaacac aaataatact aaagtaaatt taaatacggc atctgtatca gaattgatgt
    43861 ctgttcctgg agttgggcaa gctaaagcta atgcaattgt tgaatatcgc aaccaacaag
    43921 gtgcatttca agaaattgac gatttgaaaa aagtaaaagg ttttggaagt aaaacttttg
    43981 ataaactgaa atcttatttc acgacataat ggattatttt aattgcaaat ttta
//
LOCUS       DALAGF010000011        43540 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.011, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000011 DALAGF010000000
VERSION     DALAGF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 43540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43540
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.011"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(93..476)
                     /locus_tag="O2692_000557"
                     /pseudo
     CDS             complement(93..476)
                     /locus_tag="O2692_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(671..895)
                     /locus_tag="O2692_000558"
     CDS             complement(671..895)
                     /locus_tag="O2692_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HCZ4298201.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(912..1115)
                     /locus_tag="O2692_000559"
     CDS             complement(912..1115)
                     /locus_tag="O2692_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HCZ4298202.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYSNIELIKSLSI"
     gene            1274..1834
                     /locus_tag="O2692_000560"
     CDS             1274..1834
                     /locus_tag="O2692_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCZ4298203.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            1853..4255
                     /gene="mnhA2"
                     /locus_tag="O2692_000561"
     CDS             1853..4255
                     /gene="mnhA2"
                     /locus_tag="O2692_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HCZ4298204.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAV
                     SGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            4242..4667
                     /gene="mnhB2"
                     /locus_tag="O2692_000562"
     CDS             4242..4667
                     /gene="mnhB2"
                     /locus_tag="O2692_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HCZ4298205.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            4664..5008
                     /gene="mnhC2"
                     /locus_tag="O2692_000563"
     CDS             4664..5008
                     /gene="mnhC2"
                     /locus_tag="O2692_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HCZ4298206.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            4998..6494
                     /gene="mnhD2"
                     /locus_tag="O2692_000564"
     CDS             4998..6494
                     /gene="mnhD2"
                     /locus_tag="O2692_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HCZ4298207.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            6495..6977
                     /gene="mnhE2"
                     /locus_tag="O2692_000565"
     CDS             6495..6977
                     /gene="mnhE2"
                     /locus_tag="O2692_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HCZ4298208.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDW
                     SITFLTILIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR
                     "
     gene            6974..7276
                     /gene="mnhF2"
                     /locus_tag="O2692_000566"
     CDS             6974..7276
                     /gene="mnhF2"
                     /locus_tag="O2692_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HCZ4298209.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            7251..7688
                     /gene="mnhG2"
                     /locus_tag="O2692_000567"
     CDS             7251..7688
                     /gene="mnhG2"
                     /locus_tag="O2692_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HCZ4298210.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAEKREEHRKKWYQND"
     gene            8028..10070
                     /locus_tag="O2692_000568"
     CDS             8028..10070
                     /locus_tag="O2692_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCZ4298211.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(11097..12026)
                     /locus_tag="O2692_000569"
     CDS             complement(11097..12026)
                     /locus_tag="O2692_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCZ4298212.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(12023..12859)
                     /locus_tag="O2692_000570"
     CDS             complement(12023..12859)
                     /locus_tag="O2692_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCZ4298213.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            complement(12853..13596)
                     /locus_tag="O2692_000571"
     CDS             complement(12853..13596)
                     /locus_tag="O2692_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298214.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            13718..14362
                     /locus_tag="O2692_000572"
     CDS             13718..14362
                     /locus_tag="O2692_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCZ4298215.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            complement(14442..15194)
                     /locus_tag="O2692_000573"
     CDS             complement(14442..15194)
                     /locus_tag="O2692_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HCZ4298216.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            15430..16194
                     /locus_tag="O2692_000574"
     CDS             15430..16194
                     /locus_tag="O2692_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HCZ4298217.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            complement(16255..17049)
                     /gene="tagH"
                     /locus_tag="O2692_000575"
     CDS             complement(16255..17049)
                     /gene="tagH"
                     /locus_tag="O2692_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HCZ4298218.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            17396..18208
                     /gene="tagG"
                     /locus_tag="O2692_000576"
     CDS             17396..18208
                     /gene="tagG"
                     /locus_tag="O2692_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HCZ4298219.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            18307..19410
                     /locus_tag="O2692_000577"
     CDS             18307..19410
                     /locus_tag="O2692_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCZ4298220.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDQVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            19407..20468
                     /locus_tag="O2692_000578"
     CDS             19407..20468
                     /locus_tag="O2692_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCZ4298221.1"
                     /translation="MRFTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVIKPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNEE
                     DFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            20531..20929
                     /gene="tagD"
                     /locus_tag="O2692_000579"
     CDS             20531..20929
                     /gene="tagD"
                     /locus_tag="O2692_000579"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HCZ4298222.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(21046..22341)
                     /gene="pbp4"
                     /locus_tag="O2692_000580"
     CDS             complement(21046..22341)
                     /gene="pbp4"
                     /locus_tag="O2692_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HCZ4298223.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQATNSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGTCLVAGLALIVHMIINRLFRKRK"
     gene            22762..24489
                     /locus_tag="O2692_000581"
     CDS             22762..24489
                     /locus_tag="O2692_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298224.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            24848..26077
                     /locus_tag="O2692_000582"
     CDS             24848..26077
                     /locus_tag="O2692_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCZ4298225.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            26332..26424
                     /locus_tag="O2692_000583"
     CDS             26332..26424
                     /locus_tag="O2692_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298226.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            26602..27435
                     /locus_tag="O2692_000584"
     CDS             26602..27435
                     /locus_tag="O2692_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCZ4298227.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            27717..28514
                     /locus_tag="O2692_000585"
     CDS             27717..28514
                     /locus_tag="O2692_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298228.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            28550..29554
                     /locus_tag="O2692_000586"
     CDS             28550..29554
                     /locus_tag="O2692_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCZ4298229.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            29551..30567
                     /locus_tag="O2692_000587"
     CDS             29551..30567
                     /locus_tag="O2692_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCZ4298230.1"
                     /translation="MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDKLILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            30801..31769
                     /gene="dhaK"
                     /locus_tag="O2692_000588"
     CDS             30801..31769
                     /gene="dhaK"
                     /locus_tag="O2692_000588"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCZ4298231.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            31811..32395
                     /gene="dhaL"
                     /locus_tag="O2692_000589"
     CDS             31811..32395
                     /gene="dhaL"
                     /locus_tag="O2692_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCZ4298232.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            32388..32750
                     /locus_tag="O2692_000590"
     CDS             32388..32750
                     /locus_tag="O2692_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCZ4298233.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            32866..33363
                     /locus_tag="O2692_000591"
     CDS             32866..33363
                     /locus_tag="O2692_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298234.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            33819..34886
                     /locus_tag="O2692_000592"
     CDS             33819..34886
                     /locus_tag="O2692_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298235.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            35037..36080
                     /locus_tag="O2692_000593"
     CDS             35037..36080
                     /locus_tag="O2692_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4298236.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            36409..36837
                     /locus_tag="O2692_000594"
     CDS             36409..36837
                     /locus_tag="O2692_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298237.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(37046..37552)
                     /locus_tag="O2692_000595"
     CDS             complement(37046..37552)
                     /locus_tag="O2692_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4298238.1"
                     /translation="MAHIIRRVSIKDVENFISMLANIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            37665..38588
                     /gene="graX"
                     /locus_tag="O2692_000596"
                     /gene_synonym="apsX"
     CDS             37665..38588
                     /gene="graX"
                     /locus_tag="O2692_000596"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HCZ4298239.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            38604..39278
                     /gene="graR"
                     /locus_tag="O2692_000597"
                     /gene_synonym="apsR"
     CDS             38604..39278
                     /gene="graR"
                     /locus_tag="O2692_000597"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HCZ4298240.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDQTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            39271..40311
                     /gene="graS"
                     /locus_tag="O2692_000598"
                     /gene_synonym="apsS"
     CDS             39271..40311
                     /gene="graS"
                     /locus_tag="O2692_000598"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HCZ4298241.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNFLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLLLTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            40455..41216
                     /gene="vraF"
                     /locus_tag="O2692_000599"
     CDS             40455..41216
                     /gene="vraF"
                     /locus_tag="O2692_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HCZ4298242.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            41206..43095
                     /gene="vraG"
                     /locus_tag="O2692_000600"
     CDS             41206..43095
                     /gene="vraG"
                     /locus_tag="O2692_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HCZ4298243.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIY
                     ILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLI
                     GVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYL
                     SLNIVQYYDSIGTLMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSS
                     IMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQ
                     LASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLND
                     KDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKAKNKVDKSIETRSEAISSISSLTG
                     ILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIM
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRT
                     IRHSI"
BASE COUNT        14925 a         6066 c         7716 g        14833 t
ORIGIN      
        1 ttgatttgaa ccatcaaaaa aaggtgtaaa ctcagtcata attatcaatt cctttcataa
       61 gaattaactg ttaactaaag tttacaccac caattaaaat catgaattgt agcgccaaat
      121 aatccaaatg tatcaataat gacacccata ataatttgac ccgcaactgt tgcaattaca
      181 gttaatgttg cacctaattt tggcaataac aataaattgc cagttaaaaa gctaacccca
      241 agcaaaccac cgactaccca tgtgtagtta aatgattgat tattgtaaaa gtgaatagta
      301 aatacttctg gattgataat gatatttaaa ataattaaac aaattgttcc aactgaaaat
      361 gaaatgaatg aagtatagaa aggcgattta gtatacagtg atagtcttga attgacagat
      421 gtttggatag gaataagcat tccaactagg acaccaatga tatagaaaag aagcatatga
      481 ataaagtctc actttccaaa gttaataata aaatcatata tttacattat cattatagag
      541 aaaatataca actcatttac aataatgttg aaaaaaacag gacattatta caacaaaatc
      601 accctagaca atcatgatga aagtctaggg tgttagtatt tatactaaat taattgcgct
      661 ttctttatat ctatttctta tcttcaaata aaacagttgt tgttttatca ataatttgag
      721 cagaatgtac tttggtgata gtaccactat ttgactttat aatatttaaa tctactaatg
      781 cattcatact atcacgtgca acttgctcgg tcactacgtt gtttaattgt ggtaattgca
      841 gttttacagg cttgtctagt gaagatttaa aacttaattc aagtgtttta gtcatgaata
      901 tgtcctcctg attaaattga taaagatttg atgagttcga tattgctata tgtttctcca
      961 gtaagtcgct caataagttt gctgaatgtt ttaatttggt cgtttgatgc atcagggtta
     1021 atgttagcga atcgacgctt aaattctgtt tgtttgccgt tagcgtctac tttagtaaat
     1081 gataatacaa tagtgatgtg gtttatttta ctcatatttt aaaacctcct ttcacactat
     1141 atatcgaaac aaaataataa aatggctaat tttattttct atgtttaaaa tctataaaaa
     1201 aggcaataga tatatgtaac taaaatataa tatagctatg attaagacac ttaaagcaag
     1261 gggaggttga gtaatgaata aagtagaagc gattaaattt aatgatgata ttgttaaaat
     1321 gtatgaagcg ctcaagataa aatctgaacg tgactattta ttctttaagt tagctataca
     1381 tagtggattg aaagtatcag aattattaac aattacagtc tctcaagtta agagactaat
     1441 tgaaaagtgt acgttatcag aaatgtgtaa agcacatttt cattcgttga ttaaaattag
     1501 gttaccagaa acattatcga aagaactact tcaatatata gaggacagga gtctttcgaa
     1561 tgaagacgtt ctttttcaat cactacgaac aaatcaagta ttatctagac agcaagcata
     1621 tcgaataatt caccaagcat caattgaagc tggtatagat aatgtaggac taacgacatt
     1681 gcgtaagaca tttgcatatc atgcttatca aaaaggtata cctataccag tcattcaaaa
     1741 gtatttaggg catcaatctg ctattgaaac actaaatttt atcggtttag aaaatgagtg
     1801 tgaacatagt atttatattt cattacaatt atagaaacaa aggaggctaa taatgagttt
     1861 ggtttattta ttaattgcta tacttgtgat tatggcgatg atacttctaa tgtctaaacg
     1921 tagagcattg gctaaatatg ccgggtacat agcgttggtt gcacctgtaa tttcatctat
     1981 ctattttttg attcaaatac catcagtagc taaactgcaa tatctttcta cctctattcc
     2041 atggattaag acattagata ttaatttaga tttacgttta gatggtttaa gtttaatgtt
     2101 ttctcttatt atttcactta ttggaattgc agtattcttc tatgcaactc aatatttatc
     2161 ctctcgaaaa gacaatttac caaggtttta tttttattta acgttattta tgttcagtat
     2221 gattggtatt gtattatcag acaatacgat attgatgtac attttttggg aattaacgag
     2281 tgtatcatca tttttattga tttcatattg gtataacaat ggtgacagtc aatttggtgc
     2341 gattcaatca tttatgatta cagtatttgg tggattggcg ttattagttg gttttattat
     2401 gctgtatatc atgacaggaa cgaataacat cacagagata ttaggacaag cagatcatat
     2461 taagaatcat ggattgttta tccctatgat ttttatgttt ttattaggtg catttacaaa
     2521 atcagcacaa tttccatttc atatttggct acctagagca atggctgcac ctacacctgt
     2581 aagtgcttat ttacattcag ccacgatggt aaaagctggt atctttttat tacttcgatt
     2641 tacaccatta ttaggtctta gcaatatgta cgtatatatc gttacgtttg ttggtttaat
     2701 aacaatgtta tttggttcaa ttacagcttt aaaacaatgg gatttaaaag gtatcctagc
     2761 gtactctaca atcagtcaac ttgggatgat tatggctatg gtgggtatag gtggcggata
     2821 tgctcaacac caacaagacg caatagcatc tatttatgta tttgtattat ttggtgcgct
     2881 atttcatcta atgaatcatg ccatctttaa atgtgcgctt ttcatgggag taggtatttt
     2941 agatcatgaa gcaggttcaa gggatatacg aattttaagt ggaatgcgtc aactatttcc
     3001 taaaatgaat ctagtcatga cgatagcggc tctatctatg gctggagtac catttttaaa
     3061 tggattttta agtaaagaaa tgtttttaga tgcattaaca caaactggac aattatccca
     3121 atttagtttg atttcaatga tagctatcgt gtttgttggt gttattgcga gtgtttttac
     3181 attcacatat gcactataca tggtaaaaga agtattttgg acaaaatatg attctaaggt
     3241 ttttactaaa aaaaatatcc acgaaccatg gttgtttagt ttaccatctc ttatattaat
     3301 ggtgctagta cctgtaatct tttttgtacc aaatatattt gggaagggga ttatcgttct
     3361 agcattaaga gctgtatcag gtggtaatca tcaaattgat caattggcac cacatgtttc
     3421 gcaatggcat ggatttaaca taccgcttct tttaaccatc atcattattt tattgggtag
     3481 tgtactagca atcaaagtag attggaaaaa agtgttcaca ggtaaaatta gacagatttc
     3541 agtttcaaaa agctatgaga tggtatatcg acattttgaa aagtttgcta cgaagcgatt
     3601 taaacgtgtt atgcaagatc gtttaaacca atacattatt atgaccttag gcatatttat
     3661 gattatcatt ggatatggtt atattcgaat tggacttcct aaagtacatc agttacatgt
     3721 ttctgaattt ggggcattag aaattatatt agcaatcgta actgtcacaa ttggtatttc
     3781 tttaattttt atacgtcaac gactgacaat ggtcatttta aatggagtca tcggatttgt
     3841 tgtgacctta ttctttatag caatgaaagc ccctgatcta gcattgactc agctagtagt
     3901 tgaaacaata acgacgatac tatttattgt cagtttttca agattaccaa acgtgccaag
     3961 atctaacgct aacaaaaaaa gagaaataat taaaatttct gtatcactct tgatggcact
     4021 tattgttgta tcattaattt ttattacaca acaaacagat ggtttatcat caatatcaga
     4081 cttttattta aaagctgaca aactaacagg tggtaaaaat attgtaaatg cgatacttgg
     4141 tgactttaga gcattagata cattatttga aggattagtg ttaattatta ctgggctagg
     4201 tatttacaca ttattaaatt atcaagatcg gaggggacaa gatgaaagag aatgatgtcg
     4261 tgttaagaac ggtcacgaaa cttgttgtat ttattttatt gactttcgga ttctatgtct
     4321 tcttcgcagg tcataataat cctggtggtg ggtttattgg tggtttaata tttagttcag
     4381 cgtttatttt aatgtttctg gcttttaatg ttgaagaggt tttagaaagt ttaccgattg
     4441 attttagaat tttaatgatt attggagcat tggtatcatc tattactgcg ataataccta
     4501 tgttttttgg aaaaccattt ttgtctcaat atgaaacaac ttggatactt ccaattttag
     4561 gacaaattca tgtaagtaca ataacacttt ttgaattagg tattttattc tcagttgttg
     4621 gtgttattgt cacagtgatg ttgtcgctta gcggaggtcg atcatgaatt taatattatt
     4681 actagttata ggatttttag tgtttatagg aacatatatg attttatcaa tcaatttaat
     4741 tcgtattgta atcggaattt caatatatac tcatgctggt aatctcatta ttatgagtat
     4801 gggaacgtat ggttctagta gatcagaacc actaataact ggtggaaacc aattgtttgt
     4861 tgatcccttg ttacaagcta ttgtactaac tgcaatagtt atagggtttg ggatgactgc
     4921 gtttttactt gtacttgttt atagaactta taaagtaaca aaagaagatg aaattgaagg
     4981 cctaaggggg gaagatgatg ctaagtaact tattgatttt accaatgtta ttaccattcc
     5041 tttgtgcctt aatccttgta tttttaaaaa ataatgatcg tatttctaaa tatttatact
     5101 taggtacaat gactatcacc acaattattt cattaatgct attaatttat gttcagcgtc
     5161 accgtccaat tacgctagac tttggaggat ggtcagcgcc ctttggtata cagtttttag
     5221 gagattcttt aagtttaatt atggttacaa ccgcttcgtt tgtgattact ttaattatgg
     5281 catacggatt tgggcgtggc gaacataaag caaatcgtta tcacttgcca tcgttcatat
     5341 tatttttaag tgttggcgtg ataggctctt ttctaacatc agatttattt aatttatacg
     5401 tcatgtttga aattatgtta ctagcgtcat ttgtactcat tacacttgga caatctgtag
     5461 aacaattacg tgctgcaatt atttatgttg tcttgaatat tattggttca tggctattct
     5521 tattaggtat aggtttactt tataaaacag taggtacatt aaacttttca catattgcaa
     5581 tgcgtttgaa tgacatggga gataatcgca ctgttacaat gatttcatta atcttcttag
     5641 tcgcatttag tgcgaaagca gcgctggtcc tttttatgtg gctacccaaa gcctacgctg
     5701 tgttaaatac tgagcttgca gcattatttg cagcgttaat gaccaaagta ggggcctatg
     5761 cattaattcg attcttcact ttactatttg atcaacataa tgatctcata catccattgc
     5821 tagcaactat ggctgctata actatggtca tcggcgctat aggtgtcatt gcttataaag
     5881 atattaaaaa gattgcagct taccaagtca taatctcaat aggatttatc attttaggtt
     5941 taggaacaaa cacgtttgca ggtattaatg gtgcaatatt ttatttggta aatgacattg
     6001 ttgtaaaaac attgctattt tttattattg gtagtttagt ttacattaca ggctatcgac
     6061 aatatcaata tttgaatggc ttagctaaaa aagaaccttt atttggagtt gcgtttatta
     6121 taatgatttt tgctattggc ggcgtgcctc catttagtgg ctttccgggg aaagtactta
     6181 ttttccaagg tgcattgcaa aatggcaatt atattggact agcgttaatg attattacta
     6241 gtctaattgc aatgtacagt ttatttagga tactttttta tatgtatttt ggagataaag
     6301 atggggagga agttaatttt aagaaaatcc cgctatatcg aaaaagaatt ttaagtattt
     6361 tagtagttgt ggttatcgca atcggaattg ctgcacctgt tgtgttaaat gttacaagtg
     6421 atgcaactga gttgaacacg agtgatcaat tatatcaaaa acttgtaaat ccgcatttga
     6481 aaggagagga ctaaatgaat caaatagttt taaatattat cattgcattc ttatgggtat
     6541 tatttcaaga tgaagatcat tttaaattct cgactttctt ttctggatat ctaattggtt
     6601 taattgtcat ttatatatta cacaggtttt tcagcgatga tttttatgtt agaaaaatat
     6661 gggtagctat taaattttta ggtgtttatt tatatcaatt aataacatct agcattagca
     6721 cgattaatta tattcttttt aaaacaaaag atatgaaccc tggattactt tcatatgaaa
     6781 caagactaac aagtgattgg tcaataacat ttttaacaat tttaattatt ataactccag
     6841 ggtctacagt aatacgaatt tctcaagact ctaaaaagtt ttttattcat agtatcgacg
     6901 tgtcagaaaa agaaaaagat agtttgttaa gaagtattaa gcattatgaa gacttaatat
     6961 tggaggtgtc gcgatgatac aaacaataac acatattatg attattagtt cactcattat
     7021 ttttggaatt gcattaatca tctgtttatt tagattaatc aagggaccta caacagcaga
     7081 tcgtgtcgtt acatttgata caacaagtgc tgtcgtaatg tcaattgtgg gtgtgttaag
     7141 tgtacttatg ggcaccgttt ctttcttaga ttcaatcatg ctcattgcca ttatatcttt
     7201 tgtaagttct gtttcaatat cacgctttat tggtgggggg catgtgttta atggaaataa
     7261 caaaagaaat ctttagtctt attgctgctg tgatgttgtt gttaggtagt tttattgctc
     7321 ttattagtgc aataggtatc gtgaaattcc aagatgtttt cttaagaagt cacgctgcga
     7381 caaaaagttc aactttatcc gtgttattaa ctttaatcgg tgtattaatt tattttattg
     7441 tgaatacagg atttttcagt gtgcgtttat tactgtcact tgtttttatt aatttaactt
     7501 caccagtcgg catgcactta gtcgctcgcg ctgcttatcg caacggcgct tatatgtatc
     7561 gaaaaaatga tgctcacaca catgcatcaa tattattaag ttcaaatgaa caaaactcta
     7621 cagaagcatt acaattacgt gctgaaaaac gagaagagca tcgtaagaaa tggtatcaaa
     7681 acgattgaag tgttggctaa attgttaagc ataacaatgc ttttgaaaat ctgttttcaa
     7741 aattaatcat taaaagagta ctttgaaatt ggttagtcga ttttaaggtg ctcatttttt
     7801 gtattataag ttaatgttgt tatgtgaaag caagttgttt atttatacaa acaattagtg
     7861 ataaaataaa attaaataat tgcaaattta agtactgcaa tcgtagacat atatctatat
     7921 taaagtgctt ttgtggtcgc taactcaatt tatcctctaa agtataatta caacgagatg
     7981 tgatacttaa ttttcaatta aacaatactt tttagagagg tgaaagtttg gaaatatttg
     8041 aaacaattct tatatttata gctgttgtga tactaagttc gtttgtccat actttcatac
     8101 ctaaagtacc cctagcattt atacaaattt tcttgggcat gttactattt attaccccaa
     8161 tccctgttca atttaatttt gattctgaat tgtttatggt aacaatgatt gcgcctttgt
     8221 tatttgtaga aggtgttaat gtttctagag tccatttaag gaaatatatt aagccagtga
     8281 tgatgatggc attaggatta gtcattacta ctgtgatagg tgtaggttta tttattcatt
     8341 ggatttggcc agatttacct attggagcag catttgcaat tgctgccatt ctttgtccta
     8401 ctgatgcagt agcagtgcaa gcaatcacta aaggaaaggt cttgccaaaa ggagcaatga
     8461 caattcttga aggtgagtca ttattgaatg atgctgctgg tattatttca tttaaaatag
     8521 ctgttggagt attagttaca ggtgcttttt cacttgttga tgctgttcag ttgtttttaa
     8581 ttgcatcaat tggtggcgca gtggttggtt tacttatagg tatggcatta gtaaggttcc
     8641 gattaacatt gatgcgtcga ggatatgaaa acattaatat gtttacaatt attcaattgt
     8701 taacaccatt tgttacgtat ttaattgctg aattgtttca cgcatcagga atcattgcag
     8761 cagtagttgc aggacttgta catggtttcg aacgtgacag aattatgcaa gtacgtacac
     8821 aactgcaaat gagttacaat catacatgga atatactagg ttatgtttta aatggctttg
     8881 ttttttcaat attaggattt ttagtacctg aagttattat taaaattatc aaaacagaac
     8941 cgcacaattt aatcttttta ataggcatca ctattgttgt tgctttagct gtctatctat
     9001 ttagatttgt ttgggtttat gtcttatatc cttattttta tttagccatc agtccattcc
     9061 aaaaaatgat gactaaaaat gatgatgata atccaacgac tgagaaacca ccaaagcgaa
     9121 gtttatacgc tttaattatg acgttatgtg gtgtgcatgg aacaatttct ttagcaatcg
     9181 cattaacgtt accgtatttt ttagcagggc atcatgcttt tacgtataga aacgacttat
     9241 tatttattgc atctggtatg gttattatta gtttggtagt tgcgcaagta ttattgccat
     9301 tattaacgaa acctgcacct aaaacagtaa ttggcaatat gtcgtttaaa gttgctagaa
     9361 tttatatatt agaacaagtt attgattatc taaatcaaaa atctactttc gaaacaagtt
     9421 ttaaatatgg taacgtgatt aaagaatatc atgataaatt agcattttta aaaactgtag
     9481 agaaagatga tgaaaactct aaagaattag aacgtctaca aaaaattgct tttaatgtag
     9541 aaacaaaaac attagagtct ttagtagatg aaggacaaat aacgaatagt gtacttgaaa
     9601 actatatgcg ttatgctgaa agaacacagg tatatagaca agcatcatta ataagaagaa
     9661 tgattgtatt attacgaggt gctttattaa aacgaagagt acaaacgaga gtgaactccg
     9721 catcttcact tagtgttacg gataacttaa tggaattaaa taaaattaat aaattagtcc
     9781 attataatgt ggttagtcgt ttgtctaagg aaacaacaaa agataataca cttgaagttg
     9841 gaatggtttg tgacggttat ttaatgcgaa ttgaaaactt aacaccatca aatttcttca
     9901 actcagcaag tgaagatacg attactaaaa ttaaattaaa tgcattgaga gaacaacgtc
     9961 gcattttacg tgagttgatt gatacagatg aagtatcaga aggtacagcg ttaaaactaa
    10021 gagaagccat caattatgat gaaatggtta ttgtagatag tatgacgtag ttcctaatta
    10081 tgctaaaagg gattgatgaa aaactgaagg gcttttcatc aatccctttt attttagggg
    10141 aattgaatag atagttttaa actatacgaa ttattaatat ttgagattta attgaaataa
    10201 gttttaaaaa ttggaggaga tagattaagc gaagtcattt aaaggtgaag ttaagtgtat
    10261 tcacaaaaaa tagccacact catatgacat cggatgagtg tggcttaagg atctatgggg
    10321 ggaggaaacc atagatgttt actttgatag gccagattaa atatcaaagt atgcgattat
    10381 ttatagcttg atgcaaaagt ggtatgccta ttaaaagtta ctgcacatag cttttaatat
    10441 tccgttcaaa ggaaaggggc atacaattga acaatctgta atagtacttt taaccagcta
    10501 tgctaaaagt ctagtaggga gaacagttgt ccaatcacat aagaacctct aacttcgtta
    10561 gtacgattaa gaaaagcttt ttagttagta tgtaatacaa tttattgacg cgcgtgaatc
    10621 tcttttataa gagtgtgtag ggaatggcgt tgtataaatt gtattagaag aacttctaac
    10681 gcatctctgt ggttaaaaga gatgaaggga acgacagttt aattaaaact gcataagaac
    10741 ttctagcttt tctctctcgt tcaaagagaa gcagctgttc gcagtttaat caaaaccaca
    10801 taaagctttt aactttactc tttgatttaa agagtgacaa atgtttacag tttaattaaa
    10861 actgcataag aacttctagc ttttctcttt cgttcaaaga gaagttctaa taccaccata
    10921 tcgtgcgatc gggaacggta tatatattaa taggagggta atatatattt aacgcacgat
    10981 atgggactat tagccttcga ctttgttatg ttgatgtgtg gcctaaaata ttggagatac
    11041 caatatttta ggttgcatca acatcaattc atcttacttc attaaaacac agcgtgttat
    11101 ttcatgcttc cgtgtacagt ttcaatattt gatttcatca ttttgtagta agagtcacct
    11161 ttagtgcctt ctttaccgat tgaatctgtg tacacttcac caaagatatc tttcttcgtt
    11221 tcttcagata aactttccat tgctttctta tcaacacttg tttctactaa taagtgtttt
    11281 aatttgtgct ttttaacaaa ctcaatagct tgtctcattt gttcaggtgt accttgtttt
    11341 tcagtgttaa tttcccaaat ataacctggt gtaataccgt attgttttga gaagtacttg
    11401 aaggcacctt cacttgtaat catggcacgt tgttcttttg gaatgtcatt aaatttgtct
    11461 ttactgtcat tatttaattt ttccaattga gcaatgtatt tgttaccttg cttttcataa
    11521 tctgctttat gttttttgtc gttatcgata aatgtttgtt gaattgtttt tacgtattta
    11581 ataccgttat ctaaacttaa ccatgcgtgt ggatcttgtt tatctttgtt gccttcttca
    11641 ccgtttaaat agataggttt aacatctttt gatactgcga taactttttt atcttttaat
    11701 gatttaccag cctgttctaa ggctttttca aaccaaccgt taccagtctc taaatttaat
    11761 ccgttgtata aaataacgtc agcgtcagtt aactttttaa tatctttagg tttaacttca
    11821 tattcatgag gatcttgacc aacaggtaca atactatgaa tatcgacgtt gtctccacca
    11881 acatttttag ccatatcata taaaattgaa ttcgtcgtta ctacttttaa tttgccattt
    11941 gacttatcac tgctttgttt accaccagta ccacatgcag caactagaag taataaggct
    12001 aataataaag gtactaattt tttcatgtta aacttcctcg tttctttcta ttcgtaaatt
    12061 ttgtgaaaaa taatgtgatg atataaatta caaacgtaca aagtacgatt gtcgcaccac
    12121 taggaatgtt gtaaatatag ctgtaataaa gtccgacaat tgaacttatg acacttatta
    12181 aacttgctat aatcatcatt gagtatagtt ttttactaat taaaaatgct gtagatgcag
    12241 gtgtaattaa taatgcaact acaagaataa tacctaccgt ttgaatactt gctactgtta
    12301 ctaatgagag taacaacatc acaaagtaat gtaataacgt cgtatttaga ccactcattc
    12361 tactaaacgt tggatcgaat gtagaaatca ttaatggacg atagaaaata atgattagaa
    12421 taaggacgat tgaaccaatc acaatagttg ttaaaaatgc actatttgtg attgccagta
    12481 aattaccaaa cagaatatgg tacaaatctg tcgtagtgtt tattaagcta ataataataa
    12541 tccccgaagc taagaaagcg gtaaaactaa ttccaatagc ggcgtcaggt ttcgttttac
    12601 tactagatgt gatataaccg ataaaaatac ttgcgatcat accagttata agtgcgccta
    12661 caaacattgg aataccaaat aagaatgata gggcaacacc aggtaatact gcgtgactca
    12721 ttgcatctcc cattaatgaa agaccacgta atacaattaa actaccaact gtaccacaaa
    12781 ctatccctac aataattgaa gttatcaatg ctcgattcaa gaattgatat gtaaataaat
    12841 gttcgacaaa ctctaacatg ttatattgct cctttgacta gggtcactac agtcagtgct
    12901 actcataaat gtttcgttta agcgagtgac actcatagcc tcttcactat caccaaagta
    12961 tcgtaatgtt tgatttaata gaataatgcg atcaaagtat tgctttgctt ttgatagatc
    13021 atggtggatg ataagaataa gttttccttg ttgttttaag ttctcgattt ttgtcatgat
    13081 taatttttcg ctactaaaat caattccgac aaacggctca tctagaaaat aaacttcact
    13141 ttcggacatc aatgctcttg ctactagcac acgttgtaat tgtccaccac ttaattctga
    13201 aatttgtcga tgacgtaaag attctaattc taaatcgctt aataactgtt tgagtttatc
    13261 ccttgctgat ttattaggtc gtctaaacca tccaatttct ttgtagcaac ctgataaaat
    13321 cacttgttcc acacttatag gaaaatctaa atcaatatgt gctttttgtg gaatatatgt
    13381 aatatgttgc agttgttgtt gtataggttt gttatataac aatttagtac cggtagcatt
    13441 aaattcacca attaaagact tgataaggga agatttacca gcaccattcg ggcccatgat
    13501 accaattatt tcgccgcgta ctggtatcga taaggaaatg tttttaagta catgcttatt
    13561 acctaaaaac agatttaaat cttttgtttc taacaaacgt ttatacctcc taattaaaag
    13621 tttaggctaa cctaattaat tgtataataa actgagaata tttatcatgt caagtaaatt
    13681 cgtgatataa tatagacaat gtatgtgagg tgaaagtatg ttaactgaag aaaaagagga
    13741 ctatttaaag gcaatcctta cgaataatgg cgataaaaac tttgtgacaa ataaaatctt
    13801 atctcaattt ttaaatatta agcctccatc tgtaagtgaa atggtaggac gtcttgaaaa
    13861 agcaggctat gttgaaacaa aaccatacaa aggtgttaga ttaacagagg atggtttaac
    13921 gcatacgctt gatatcatta agagacatcg actattagaa ttatttttaa tagaaatatt
    13981 gaaatataat tgggaagaag tacatcaaga agcagaaatt ttagaacatc gaatttcaga
    14041 tttatttgtt gaaaggctgg atagcctgtt aaatttccca gaaacttgcc cgcacggcgg
    14101 tgtgattcct agaaataatg aatataaaga gaaatatata acaacgattt tgaattatga
    14161 acctggtgat atcgttacaa tcaaacgtgt gagagataag accgatttgc taatatattt
    14221 gtctagtaaa gatatttcta ttggtaatga agtggaaatt gtatcgaaag atgaaatgaa
    14281 taaagtaatt atcattaaac gtaatgataa tgtaattatt gtcagttacg aaaatgcaat
    14341 gaacatgttt gctgaaaaat aaaataaaga agccataaag atatccatga ttgaactgat
    14401 aaagacatat ggataattgc tttaggcttc ttttttatta gttaatttat caagtgagta
    14461 tatttgagta aaatattcac tgcataaaga ttgaagataa tccagattgt actataaatg
    14521 aagataggta catgactgag ttctttaagt gcactaccat cccactgtgg actcggacgc
    14581 tggaaagtca atttagcaat cgtccaacta gattgtagaa cttcgcctaa taatacacct
    14641 aaaatatatt gataactcat tgtgacaagt agttgaattt ctactatatt ttcatctttt
    14701 aatataaaat acaacatgat agaaattaaa gttataacaa caatgggtga gccttttcta
    14761 gatgttaaaa ttaaaaaata aataaatatc aataaatagg taaatataaa gaaactaggt
    14821 atctgataat ggctcgacgc taaacctatc aataacataa taggtggcat aaaataacca
    14881 ccaatcgttg taagccattg gcctgctaga tgtctagatt gtgtaattgc gaatccttgt
    14941 tgtaatgtct gttgtcgctc tcgtggactt gttacaatga ctaaatcttt tgcacggcca
    15001 ccagcgagtt tattaaacag tacatgacca aattcatgtg ttaaaacagg gatatagttt
    15061 aaaatgacat ctaaatagtt caaaacaggc ttatgtctat attgatgaat agcaatataa
    15121 caagctgcaa caataacgat aatgtatata ttaagttgaa ttgtcgtatt aaaaaagttt
    15181 gataaataat tcattgttaa cctcatataa gatattaatt taaagtttgc ttatcactta
    15241 ttataaatga tattggcatc aatagcgtta gactttagac ttaccttagt taaactaatt
    15301 ttaatttttg aaaaggtgaa tatgtgttaa aataaagcaa aatcatttcg atataaatag
    15361 gatgaatata aatactgtta atattgatta cactaacata ataatgaaat aagataggag
    15421 attcctgtta tgactgttga agaaagatcc aatacagcca aagttgacat tttaggggtc
    15481 gattttgata atacaacaat gttgcaaatg gttgaaaata ttaaaacctt ttttgcaaat
    15541 caatcaacga ataatctttt tatagtaaca gccaaccctg aaatagtgaa ttacgcgacg
    15601 acacatcaag cgtatttaga gttaataaat caagcgagct atattgttgc tgatgggaca
    15661 ggagtagtca aagcttcgca tcgtttaaag caacctctag cgcatcgtat acctggtatt
    15721 gagttgatgg atgaatgttt gaaaattgct catgtaaatc atcaaaaagt atttttgcta
    15781 ggggcaacta atgaagttgt agaagcggca caatatgcat tgcaacaaag atatccaaac
    15841 atatcgtttg cacatcatca cggttatatt gatttagaag atgagacagt agtgaaacga
    15901 attaaactgt ttaaacctga ttacatattt gtaggtatgg gattccctaa acaagaagaa
    15961 tggattatga cacatgaaaa ccaatttgaa tctacagtga tgatgggcgt aggtggttct
    16021 cttgaagtat ttgctggggc taaaaagaga gcgccttata tctttagaaa attaaacatt
    16081 gaatggatat atagagcatt aatagattgg aaacgtattg gtagattaaa gagtattcca
    16141 atatttatgt ataaaatagc caaagcaaaa agaaaaataa aaaaggcgaa ataatcatga
    16201 tgacaaaaat aaaaccgagg aaatccttaa atggagattc tcggtttttt cggtttattt
    16261 aataacgaag cgggactcat cgagtttgtt tctaaattct ttttgttcgg ctttggattt
    16321 ctttttaaaa tcgttaagga aagcttcata tttaggtaat acatcatcaa gttcaccgta
    16381 atcttttaac tttccgcctt caatccaagc aatcttagta caaaattgtc tcacttgtcc
    16441 taagttatga ctaacgaaaa agatggtttt gttttgctct ttaaactcgt aaattttatc
    16501 taaacatttt tgtgcaaaag tttggtcacc tacagataaa gcttcgtcaa tgactaagat
    16561 atctggatta actgtgatat taattgaaaa accaagtttt gcacgcatac cacttgaata
    16621 ctttttaact ggttgataaa taaactcacc aagttcacta aattcaataa tcttaggtgt
    16681 catcgcttta atttcttttc gcttaaagcc catacataac attttaaatt cgatattttc
    16741 aatccctgta agttgtccac tcaagccagc actaattgcg ataacgctga cttcaccatt
    16801 acgatccact ttgccaacag taggcgacaa agaaccgcca atgatattgc tcaacgttga
    16861 tttgccggaa ccattgatgc caacaagccc tatgacgtca ccttcatatg cttttaaact
    16921 aatgtcatct aaagcgaaaa atgttttgtt tttatgtttg ggaatgagcg catctttcat
    16981 acgttcttta tttgtacgat aaatacgata ttcttttgtt acatttttaa tgtttaccga
    17041 aacgttcatt tgtagacctt ccttattcac atttatctag attataatat actactcaac
    17101 agttgttaaa ttttaaaacc tgttgtaaag tgtatagaag attttgttat tatcagagtg
    17161 ggtgttttga cacaaaatgt taatcatcaa tgataacaat gatatttaaa aactaaactt
    17221 atttcaactt acatgattgt atactataat gtatttgtaa taaactaata ttttaaagaa
    17281 ctagacaata attttgatag catccatgta tagtgatagt atttacaaca attattataa
    17341 tactatttag ttaagtagag aaatagttaa acatttgaaa gtgtggttta atggaatgtc
    17401 agcaatagga acagttttta aagaacatgt aaagaacttt tatttaattc aaagactggc
    17461 tcagtttcaa gttaaaatta tcaatcatag taactattta ggtgtggctt gggaattaat
    17521 taaccctgtt atgcaaatta tggtttactg gatggttttt ggattaggaa taagaagtaa
    17581 tgcaccaatt catggtgtac cttttgttta ttggttattg gttggtatca gtatgtggtt
    17641 cttcatcaac caaggtattt tagaaggtac taaagcaatt acacaaaagt ttaatcaagt
    17701 atcgaaaatg aacttcccgt tatcgataat accgacatat attgtgacaa gtagatttta
    17761 tggacattta ggcttacttt tacttgtgat aattgcatgt atgtttactg gtatttatcc
    17821 atcaatacat atcattcaat tattgatata tgtaccgttt tgttttttct taactgcctc
    17881 ggtgacgtta ttaacatcaa cactcggtgt gttagttaga gatacacaaa tgttaatgca
    17941 agcaatatta agaatattat tttacttttc accaattttg tggctaccaa agaaccatgg
    18001 tatcagtggt ttaattcatg aaatgatgaa atataatcca gtttacttta ttgctgaatc
    18061 ataccgtgca gcaattttat atcacgaatg gtatttcatg gatcattgga aattaatgtt
    18121 atacaatttc ggtattgttg ccattttctt tgcaattggt gcgtacttac acatgaaata
    18181 tagagatcaa tttgcagact tcttgtaata tatttatatg acgaaacccc gctaaccatt
    18241 aataaatgga agtggggttc atttttgttt ataatttaag taaataacat attaagttgg
    18301 tgtattatga acgttttaat aaagaaattt tatcatttgg tagttcgaat actttctaaa
    18361 atgattacgc ctcaagtgat tgataaaccg catatcgtat ttatgatgac ttttccagaa
    18421 gatattaagc ctatcatcaa agcattaaat aattcgtcgt atcagaaaac tgttttaaca
    18481 acaccaaaac aagcgcctta tttatctgaa cttagcgacg atgttgatgt gatagaaatg
    18541 actaatcgaa cattggtaaa acaaattaag gctttgaaaa gcgcgcagat gattattatc
    18601 gataattatt acctattact aggtggatat aataagactt ctaatcaaca cattgttcaa
    18661 acgtggcatg caagtggtgc attaaaaaac tttggcttaa cagatcatca agtcgatgtg
    18721 tctgacaagg caatggttca gcagtaccgt aaagtttatc aagcgacgga tttttactta
    18781 gtgggttgtg aacaaatgtc acaatgtttt aaacagtctt taggtgcaac agaagagcaa
    18841 atgctgtatt ttgggcttcc gagaattaat aaatattaca cagctgatag agcaacggtt
    18901 aaggcagagt taaaggataa atatggaatt acaaataagt tggtattata tgtaccaaca
    18961 tatagagaag ataaagcaga taatagggct attgataaag cttattttga aaaatgttta
    19021 ccaggatata cactgattaa taaattacat ccatcaattg aagattcaga cattgatgac
    19081 gtatcttcaa tcgacacgtc tacattaatg ctaatgtcag atataattat tagcgactat
    19141 agttcgctgc caatagaagc tagcttgtta gatattccaa ctatatttta tgtgtatgat
    19201 gaaggaacat atgatcaggt gagaggcctg aatcaatttt acaaagcaat accggatagc
    19261 tacaaagtgt atactgaaga agatttaata atgacgatac aagaaaaaga acatctatta
    19321 agtccgttat ttaaagattg gcataagtat aatactgata aaagtttaca tcagctcaca
    19381 gaatatatag ataagatggt gacaaaatga ggtttacgat aatcatacct acatgtaata
    19441 atgaggcaac aattcgacaa ttgttaatat ctattgagag taaagaacac tatagaatcc
    19501 tttgtattga tggtggttct actgatcaaa caattcctat gattgaacgg ttacaaagag
    19561 aactcaagca tatttcatta atacaattac aaaatgcttc gatagctacg tgtattaata
    19621 aaggtttgat ggatatcaaa atgacagatc cacatgatag tgacgcattt atggtcataa
    19681 aaccaacatc aatcgtattg ccaggtaaat tagataggtt aactgctgct ttcaaaaata
    19741 atgataatat tgatatggta atagggcagc gagcttacaa ttaccatggt gaatggaaat
    19801 tgaaaagtgc tgatgagttt attaaagaca atcgaatcgt tacattaacg gaacaaccag
    19861 atttgttatc aatgatgtct tttgacggaa agttattcag tgctaaattt gctgaattac
    19921 agtgtgacga aactttagct aacacataca atcacgcaat acttgtcaag gcgatgcaaa
    19981 aagctacgga tatacattta gtttcacaga tgattgtcgg agataacgat atagatacac
    20041 atgctacaag taacgaagaa gattttaata gatatatcac agaaattatg aaaataagac
    20101 aacgagtcat ggaaatgtta ctattacctg aacaaaggct attatatagt gatatggttg
    20161 atcgtatttt attcaataat tcattaaaat attatatgaa cgaacaccca gcagtaacgc
    20221 acacgacaat tcaactcgta aaagactata ttatgtctat gcagcattct gattatgtat
    20281 cgcaaaacat gtttgacatt ataaatacag ttgaatttat tggtgagaat tgggatagag
    20341 aaatatacga attgtggcga caaacattaa ttcaagtggg cattaatagg ccgacttata
    20401 aaaaattctt gatacaactt aaagggagaa agtttgcaca tcgaacaaaa tcaatgttaa
    20461 aacgataacg tgtacattga tgaccataaa ctgcaatcct atgatgtgac aatatgagga
    20521 ggataactta atgaaacgtg taataacata tggcacatat gacttacttc actatggtca
    20581 tatcgaattg cttcgtcgtg caagagagat gggcgattat ttaatagtag cattatcaac
    20641 agatgaattt aatcaaatta aacataaaaa atcttattat gattatgaac aacgaaaaat
    20701 gatgcttgaa tcaatacgct atgtcgattt agtcattcca gaaaagggct ggggacaaaa
    20761 agaagacgat gtcgaaaaat ttgatgtaga tgtttttgtt atgggacatg actgggaagg
    20821 tgaattcgac ttcttaaagg ataaatgtga agtcatttat ttaaaacgta cagaaggcat
    20881 ttcgacgact aaaatcaaac aagaattata tggtaaagat gctaaataaa ttatatagaa
    20941 ctatcgatac taaacgataa attaacttag gttattataa aataaatata aaacggacaa
    21001 gtttcgcagc tttataatgt gcaacttgtc cgtttttagt atgttttatt ttctttttct
    21061 aaataaacga ttgattatca tatgaacaat aagtgctaat ccagcgacaa ggcatgtacc
    21121 accaatgata gtgaataatg gatgttcttc ccacatactt ttagcaacag tatttgcctt
    21181 ttgaataatt ggctgatgaa cttctacagt tggaggtcca taatctttat taataaattc
    21241 tcttggatag tccgcgtgta ctttaccatc ttcgactaca agtttataat cttttttact
    21301 aaaatcactt ggtaaaacat cgtaaagatc attttcaaca taatatttct taccatttat
    21361 cctttgctca cctttagaca atatttttac atatttatac tgatcaaatg agcgttccat
    21421 taatgcattc cccatcatat tacgttgctt ctcgccacca aggtttttat agtctcctgc
    21481 acccatgata acttgattaa ttctaaattt acctcgtttg gtagtaatcg tatggttgta
    21541 atttgctgta tcacttgatc cagtttttaa accatctgta cccggcaaac tcatttttgc
    21601 accttccaat gaaaagttga atgtgtaata cgtaactgca tgcgttgttg gtgctaactg
    21661 ctttgtaaag tctaatattt taggtgtctc tttaatcacg tgtaaatcta aaatggcata
    21721 gtctctagca gtcgttacag tacgttcttg gtctttatac tttgttggtg caaatgtacg
    21781 taatcttgaa ttttcagcac ccgttggatt gacgaaatgt gtatttttca ttccgatagc
    21841 tttagctttg ttattcatta aatcaacgaa atcgctggtg ttttttgaaa ccttcttagc
    21901 taaaattaat gccgcggcat tactagaatt agatactgta atttgtaata ggtctgcgat
    21961 tgtccatact tgtccaggat atagtttcgt attactcaac tcaggtagtg tagacataat
    22021 atattctttg ttcgtcattg tgactgtgtc atcaagtgaa agctgcccct tatttacagc
    22081 ttccaatgtt aagtacattg tcattaattt agtcatagac gctggattcc acttagtatc
    22141 gatattgtat tgatacagta attgtccagt ttgacttaca ttaacagcac tcgtcggttc
    22201 gtatgcagcc gacaaacctg cataaccata ttgatttgct gcttgtacag gggttacgtc
    22261 actgttagta gcttgtgcat atggtgtcat aatacttaat gttaaacata aaatgatgat
    22321 aatagatatt aaatttttca taaagcgtta atcttccctt ttccaattct taaatattcc
    22381 ctaaaagcaa tggttattcc tacttacgga aatcattgct aattcacttc accttaatta
    22441 aattgttgaa aataaagttt tctgcagtta atttgaaaaa taatgcaaat atattacgtg
    22501 tgtagctaaa ggtgttataa tgtttgtacg aagagcaaac ttactcaaaa gcgattaatt
    22561 ttcatgtttt aatataaaga ctttgagaag ttattacaaa aaatgcaata gaaatattct
    22621 atcatataaa tgttatgagc ggtattttgg ggcaacactt tatttgattt ttaaagtttt
    22681 gttgggagaa agtatatgat agaaatgcat gtatctatct aaatgaatta actataaatt
    22741 tcaaacagaa gaggtaaaac tatgaaacga gaaaatccat tgtttttctt atttaaaaaa
    22801 ctatcatggc cagtgggtct tatcgttgca gctatcacta tttcatcact agggagctta
    22861 agtggactat tagtgccact gtttactgga cgaattgtag ataaattttc cgtgagccat
    22921 atcaattgga atctaatcgc attatttggt ggtatctttg tcatcaatgc tttattaagc
    22981 ggattaggtt tatatttatt aagtaaaatt ggtgaaaaga ttatttatgc gatacgctca
    23041 gttttatggg agcatatcat acaattaaaa atgccattct ttgacaaaaa tgaaagtggt
    23101 caattaatga gtcgattaac tgacgatacg aaagtgataa atgaatttat ttcacaaaag
    23161 ctacctaact tattaccatc aatcgttaca ttagttgggt cactaatcat gttatttatt
    23221 ttagattgga aaatgacatt attaacattt ataacgatac cgatattcgt tttaattatg
    23281 attcctctag gtcgtattat gcaaaagata tcgacaagta cacaatctga aattgcaaac
    23341 ttcagtggtt tgttagggcg tgtcctaact gaaatgcgtc ttgttaaaat atcaaataca
    23401 gagcgtcttg aattagataa tgcacataaa aatttgaatg aaatatataa attaggttta
    23461 aaacaggcta aaattgcggc agttgtacaa ccaatttcag gtatagttat gttgctaaca
    23521 attgcaatta ttttaggttt tggtgcatta gaaattgcga ctggtgcaat cactgcaggt
    23581 acattaattg caatgatatt ttatgttatt cagttatcta tgcctttaat caatctttcc
    23641 acgttagtta cagattataa aaaggcagtc ggtgcaagta gtagaatata cgaaatcatg
    23701 caagaaccta ttgaaccgac agaagctctt gaagattctg aaaatgtatt aattgatgac
    23761 ggtgtattgt catttgaaca tgtagacttt aaatatgatg tgaagaaaat attagatgat
    23821 gtgtcgttcc aaatcccaca aggtcaagtg agtgcttttg taggcccttc tgggtctggt
    23881 aaaagtacga tatttaatct gatagaacgt atgtatgaaa ttgagtcagg tgatattaaa
    23941 tatggccttg aaagtgtcta tgatatcccg ttatctaagt ggcgacgcaa aattggatat
    24001 gttatgcaat caaattcgat gatgagtggt acaattagag acaatatttt atacggaatt
    24061 aatcgtcatg tttcagatga agaacttatt aattatgcta aattagcgaa ctgtcatgat
    24121 tttatcatgc aatttgatga aggatatgac acgcttgtag gtgaacgagg attgaaactg
    24181 tctggcggac aacgtcaacg tattgatatt gctagaagtt ttgttaaaaa tcctgatatt
    24241 ttgttacttg atgaagcaac agctaatctc gatagtgaaa gtgaattgaa aattcaagaa
    24301 gctttagaaa cattgatgga aggtagaaca acgattgtca ttgcgcatcg tttgtctaca
    24361 attaaaaaag ccggtcaaat tatattctta gacaaaggac aggtaacagg taaaggtacg
    24421 cattcagaac tgatggcatc acatgcgaag tataaaaact ttgtagtgtc tcaaaaatta
    24481 acagattaat tttatatata taagtaagct tggagcaaat acacatatac catcgaggaa
    24541 attaaagtgt ggcacattga tggatataga tgttaataaa ttgcttcaag cttttgtcta
    24601 ttttaaatca tttgagaagt tacgacataa taattcttaa attaatgaaa tcgatatttt
    24661 aagaaaaaaa tgctcatggt ataatacaag ttataagcaa acatacatat attaaatact
    24721 gtagccacga gtcataattc ttcgtatttt acatagcaat ttaactgatt ttagagtcca
    24781 cggtacagaa gtttgatatt tcaatgtttc taaattttta aaaaattaaa tcataggtgg
    24841 gtgccaaatg tttttattaa tcaacattat tggtctaatt gtatttcttg gtattgcggt
    24901 attattttca agagatcgca aaaatatcca atggcaatca attgggatct tagttgtttt
    24961 aaacctgttt ttagcatggt tctttattta ttttgattgg ggtcaaaaag cagtaagagg
    25021 agcagccaat ggtatcgctt gggtagttca gtcagcgcat gctggtacag gttttgcatt
    25081 tgcaagtttg acaaatgtta aaatgatgga tatggctgtt gcagccttat tcccaatatt
    25141 attaatagtg ccattatttg atatcttaat gtactttaat attttaccga aaattattgg
    25201 aggtattggt tggttactag ctaaagtaac aagacaacct aaattcgagt cattctttgg
    25261 gatagaaatg atgttcttag gaaatactga agcattagcc gtatcaagtg agcaactaaa
    25321 acgtatgaat gaaatgcgtg tattaacaat cgcaatgatg tcaatgagct ctgtatcggg
    25381 agctattgta ggtgcgtatg tacaaatggt accaggagaa ctggtactaa cggcaattcc
    25441 actaaatatc gttaacgcga ttattgtgtc atgcttgttg aatccagtaa gtgttgaaga
    25501 gaaagaagat attatttaca gtcttaaaaa caatgaagtt gaacgtcaac cattcttctc
    25561 attccttgga gattctgtat tagcagcagg taaattagta ttaatcatca tcgcatttgt
    25621 tattagtttt gtagcgttag ctgatctatt tgatcgtttt atcaatttga ttacaggatt
    25681 gatagcagga tggataggca taaaaggtag tttcggttta aaccaaattt taggtgtgtt
    25741 tatgtatcca tttgcgctat tactcggttt accttatgat gaagcgtggt tggtagcaca
    25801 acaaatggct aagaaaattg ttacaaatga atttgttgtt atgggtgaaa tttctaaaga
    25861 tattgcatct tatacaccac accatcgtgc ggttattaca acattcttaa tttcatttgc
    25921 aaacttctca acgattggta tgattatcgg tacattgaaa ggcattgttg ataaaaagac
    25981 atcagacttt gtatctaaat atgtacctat gatgctatta tcaggtatcc tagtttcatt
    26041 attaacagca gctttcgttg gtttatttgc atggtaatat gtcgaagagt gactatgata
    26101 atacatttta actaataaat atgtccaggc atgtcgtcta ttgatatagg tgagatgctt
    26161 ggactttttt attattgata taaaggtatt taaatatttt taaagttacc gaaattgaag
    26221 cattataaaa accaagtgca catggtaata cacttggctt ttatgggaaa tgaatattat
    26281 tgtacatatg acagtaagga ctaggtacag tcatagtact tcgagcaaaa tttgttttgt
    26341 tattataaac aacacaaagg agataacttc tctattgaag aagttaaaaa cattatagca
    26401 gacaatgaaa tgaaagtaaa ttaaaaattc agaatatttt taattattat attgtgagtg
    26461 atatttatta gggaaagcta ttcttcatat aaattagtta aatagtaaat ctttgttaga
    26521 actctttcca tagagttgtg acagcatttt tgttgtgcta ctatttttat agtctaataa
    26581 atatataaag gggatggttt cgtgaataaa acggttaaag atttaatact agttgtctta
    26641 ggttcattta tctttgctgc aggtgtaaat gcatttatta tttctggtaa cttaggtgaa
    26701 ggcggggtta caggtttagc aattatttta tattatgcgt ttcatatttc accagccatc
    26761 actaacttct tggtcaacgc agtattgatt gccataggtt ataaattttt gagtaagaga
    26821 agtatgtact taactattct tgtaacaatt cttatttcaa tatttttgag tttaacagaa
    26881 tcatggcaag tagaaactgg aaacagcatt gtgaatgcca tttttggtgg tgtaagcgtt
    26941 ggactaggaa tcggagtaat tatccttgca ggcggtacaa cagcaggtac aacaattttg
    27001 gcgagaattg caacgaaata cctcgatgta agcacgccat atgctttgct tttcttcgat
    27061 atgatcgttg ttgcaatttc acttacagtt attccacttg ataaagtatt agtaacagta
    27121 atatcacttt atataggaac aaaagtgatg gaatatgtca tagaaggttt aaacactaaa
    27181 aaagctatga cgattatttc aactaatccc gacaaacttg ccaaagcaat agacgagcaa
    27241 attggaagag gtttaaccat tttaaacgga catggctatt atacgcgtga agaaaaagat
    27301 gtcttatacg ttgttatttc taaaacacaa gtttcaaaag caaagcgatt aattaaacaa
    27361 atcgataaag atgcattcct cgtaattcat gatgtaagag atgtctatgg taatggcttt
    27421 cttgcagatg aataaataaa tggtatgagc acacatactt aaatagaagt ccacggacaa
    27481 gtttttgaac tatgaagact tatctgtggg cgttttttat tttataaaag taatatacaa
    27541 gacatgacaa atcgagctat ccaatttaaa aagtaatgtt agtcaataag attgaaaaat
    27601 gttataatga tgttcatgat aatcattatc aattgggatg tctttgaaaa ttgataattt
    27661 aaaaatagaa attatttttt ataaacagaa agaattttat tgaaagtagg gaaattatga
    27721 atcgtttgca tggacaacaa gttaaaattg gttacgggga taacacgatt ataaataaat
    27781 tagatgttga aataccagat ggcaaagtga cgtcaatcat tggtcctaac ggctgcggga
    27841 aatctacttt gctaaaggca ttgtcacgtt tattggcagt taaagaaggc gaagtatttt
    27901 tagatggtga aaatattcat acacaatcta cgaaagagat tgcaaaaaaa atagccattt
    27961 tacctcaatc acctgaagta gcagatggct taactgttgg ggaattagtt tcatatggtc
    28021 gttttccaca tcaaaaagga tttggtagat taactgctga ggataagaaa gaaattgatt
    28081 gggcaatgga agttacagga actgatacat tccgacaccg ttcaatcaat gatttaagtg
    28141 gtggtcaaag acaacgtgtt tggattgcaa tggcattagc acaaagaact gatattatct
    28201 ttttagacga accaacaaca tatttagata tctgtcatca attagaaata ctagaattag
    28261 ttcagaagct aaatcaggaa caaggttgta caattgtcat ggttcttcat gatatcaacc
    28321 aagcgattcg tttctcagat catcttattg cgatgaaaga aggggatatc atcgctacag
    28381 gttcaacaga agacgtatta acacaggaaa tattagaaaa agtttttaat attgatgttg
    28441 ttttaagtaa agatcctaaa actggaaaac ctttactggt aacttatgac ttatgtcgca
    28501 gagcttattc ttaattaagt aagttaatat gataaaaagg acaattaaca tgacaaatag
    28561 agagaaccca acgccattga agtttttatc ctatattata ggtttaagta tgatactact
    28621 aatcacacta tttatttcta cattaatagg tgacgccaaa attcaagcct ctacaattat
    28681 agaggctatt tttaattata atcctagcaa tcaacagcaa aacatcatca atgagattag
    28741 gattcccaga aatatagcag cagtaattgt aggtatggcg cttgcagttt ctggtgcgat
    28801 tatacaaggt gttactcgta atggtcttgc tgatccggcg ctcataggtt taaattcagg
    28861 tgcttcattt gctttagcat taacatatgc agttttacca aacacttcat ttttaatatt
    28921 gatgtttgct ggatttttag gtgctattct aggaggtgct attgtattaa tgataggccg
    28981 atctagacgt gatggattta atccgatgcg tattatttta gcgggtgcag cagtaagtgc
    29041 tatgttaaca gcgctaagtc aaggtattgc attagctttt agactaaatc aaacagtaac
    29101 attttggact gctggaggcg tttcaggcac aacatggtca caccttaagt gggcaattcc
    29161 attaattggt attgcgttat tcattatatt aacaattagt aaacaactta ccattttaaa
    29221 tcttggtgaa tcattagcta aaggtttagg tcaaaatgta acaatgatca gaggcatatg
    29281 tttaattatt gctatgattc tagcaggtat tgcagttgct atcgctggac aagttgcatt
    29341 tgtaggtttg atggtacctc atatagcaag atttttaatt ggaactgatt atgctaaaat
    29401 tctaccatta acagccttgt taggtgggat actcgtgctt gttgccgatg tgatagcacg
    29461 atatttagga gaagcgcctg ttggtgcaat catttcattt atcggtgttc cttacttttt
    29521 atatttagtt aaaaaaggag gacgctcaat atgattagtt caaataataa acgcagacaa
    29581 ttgatagcac tggctgtttt tagcattcta ctatttctag gttgtacttg gagtattacc
    29641 tcaggtgaat acaacatacc tgttgaaaga tttttcaaaa ctttaattgg acaaggtgat
    29701 gccattgata agttaatctt attagatttc aggttacctc ggatgatgat tactattttg
    29761 gctggcgcag cgcttagtat tagtggtgca atagtgcaaa gtgtcacaaa aaatccaata
    29821 gctgaaccag gtatattagg tattaacgca ggtggcggat ttgcaatcgc attatttatt
    29881 gcaattggta aaattaatgc tgacaacttt gtttatgtac tgccgttaat aagtatacta
    29941 ggtggtatca ccactgcatt gattattttt attttcagtt ttaataaaaa tgaaggtgtt
    30001 acacctgcga gtatggtatt aataggtgta ggtttacaaa cagcattata tggtggctca
    30061 attacaatta tgtcaaaatt tgatgataag caatctgatt tcatcgctgc ttggtttgca
    30121 ggtaatattt ggggtgacga atggccattt gtcattgcat ttttaccgtg ggtgttgatt
    30181 attattcctt acttactatt taaatcgaat acactaaata ttattcatac gggtgataat
    30241 attgcacgag gtctaggtgt aaggttaagc agagaacgtt taatattatt ctttatcgca
    30301 gtgatgttat catctgctgc tgtagcagta gcaggttcaa tttcgtttat cggattaatg
    30361 ggtccgcata ttgccaaacg tatcgttgga ccacgtcacc agttgttttt accaattgcc
    30421 attttagtag gggcatgttt acttgttata gctgatacaa ttggcaaaat tgtattacaa
    30481 ccaggtgggg ttccagcagg tattgtcgta gcaattattg gtgcaccgta tttcttatat
    30541 ttaatgtaca aaacgaaaaa tgtatagtgt caatggacac aacttattgc tatgaaaggc
    30601 actttattat aaggcttttc atagcatttt ttatttaatg agccactcaa gactatttat
    30661 tttttcaata atgaaccatt aagttatcaa gaggatctta tcaaaaatat atttgataac
    30721 ggtatcaggt taattcttta tgatagcgca ttcatttatt ctgttttata ctatgactga
    30781 taataccaag gaggtacaac atgatgaaaa agttaatcaa taaaaaagaa acatttttaa
    30841 ctgatatgct tgaaggattg ttaattgcgc acccagagtt agatctgatt gctaatacag
    30901 ttattgtaaa aaaagctaag aaagaacatg gtgtagcaat agtctctgga ggtggaagcg
    30961 gacatgaacc tgcgcatgcc ggttttgttg cagaaggtat gctagatgca gcggtttgtg
    31021 gcgaagtatt tacatcacct acacctgata aaatattaga agctattaaa gcagtagata
    31081 ctggtgatgg tgtattacta gttgtaaaaa actatgcagg tgacgtgatg aatttcgaaa
    31141 tggcacaaga gcttgcagaa atggaaggta taaatgttca aactgttatt gttcgtgacg
    31201 acattgctgt gacaaacgaa gtacaacgtc gtggtgttgc aggaacagtg tttgttcata
    31261 agcttgccgg ttatcttgct gaaaaaggtt attcattaac agagataaaa tcgcgtgtag
    31321 aagcgttgtt acctgaaatt aaaagtattg gtatggcaat tgagccaccg cttgttccaa
    31381 ctactggaaa atatggcttt gatattgaag acgacaaaat ggaaatcggt attggtatac
    31441 atggtgaaaa aggtattcat agggaagaag taaaggatat tgatcatatt gttggaacat
    31501 tgttagacga attgtataaa gaagttactg ccaatgatgt catattaatg gtaaatggta
    31561 tgggtggtac gccgttatct gaattaaata tcgtaactaa atatattcaa caaaatttag
    31621 ctgcaagaac ggttaatgtt gctaaatggt ttgttggtga ttatatgaca tctttagaca
    31681 tgcaaggttt ttctataact atcgtgccta ataaaccaga atatttggaa gcatttttag
    31741 caccaacaac aagtcaatac tttaaataag aagtgaatat gaataaatat acatttatga
    31801 ggtggcacaa atgaaagtga atgatatgaa agcacgttta ttaaatttag aagaaacgtt
    31861 taaaaaacat gaatctgaat taactgaatt agatcgagca attggtgatg gtgaccacgg
    31921 ggttaacatg gttcgtgggt ttagtagtct taaagacaaa cttgatgata gctcaatgca
    31981 atcattgttc aaatcaactg gtatggcatt gatgtcaaat gttgggggtg catcaggacc
    32041 actgtatggc tttagctttg ttaaaatgtc tgcagtcacc aaagatgata tggataatca
    32101 agatttcatt acactaattc aggcatttgc cgaagcggtt gaatcacgtg gtaaagttac
    32161 tttaaatgaa aagacaatgt atgatgtagt agcgcgagca gcagagaagc ttaaaaatgg
    32221 tgaaacttta acattcaatg atttacagca attagcagat aatacaaaag atatggtagc
    32281 aacgaaaggt agagctgcat attttggaga agaatcaaaa ggttatattg atccaggtgc
    32341 tcaaagtatg gtttatattt taaacgcttt gattggagat gaagataatg cctaaaatta
    32401 tacttgttag ccacagtaaa gaaattgcaa gtggtacaaa atctttgtta aagcaaatgg
    32461 caggtgacgt tgatattata ccaatcgggg gattaccaga tggttcaatt ggaacttcat
    32521 ttgatatcat ccaagaagtt ttgactaaat tagaggatga tgcattgtgt ttttacgata
    32581 ttggatcttc agaaatgaat gtagatatgg caattgaaat gtatgatggt aatcatcgtg
    32641 tgttaaaagt tgatgcacca attgttgaag gcagttttat cgcagcagta aagctatcaa
    32701 tcggcggttc aattgatgat gcattagcag aaatcaaaca atcattttag ttaaaattta
    32761 ctaataatga aaaatgtaaa cctttttcaa atgaaacttt ataaaaaata tgatagtata
    32821 tatgtaaatg tttaataaaa tctggagaaa taggaggaca ttgccatgca acaccttata
    32881 aaaaaacatg tattgaatgg cgagtttgat ttagtacgac aattgatgtc cgaaacagat
    32941 tttatggaat ttgaagaagc atatatttca agtgcgcatg aagtagaaag tatgatgttt
    33001 tatacatgta ttttagatat gattaagtac gaagaatcat ctgaaatgca tgacttagca
    33061 tttttattgc ttgtgtatcc actaagtgaa tatgaaggtg ctttggattc tgcttattat
    33121 catgcagacg cttccataaa acttactgac ggcaaagaag ttaaaagttt gttacaaatg
    33181 ttattattgc atgcgatacc aacacctgtt atttcagata agaaggcttt tgatatcgcc
    33241 aagcaaattt taaaattaga tcctaataat aatgttgctc gtaacgtctt aaaagacact
    33301 gccaaacgta tggacaacgt tgttgttgat ataaatgaat tacaccaacg taatgcacgt
    33361 taattacatt tcaattatat tagcttaata atagttttaa catttggttg ggttgggcat
    33421 atgttccagc cttttttaat acttaaaaac taacgaagta tacttgtgtg cacaaatggt
    33481 ttttatacaa cattttataa atttatacat tttaataaag aacatacgat agatggttta
    33541 aaccttgtta actgagaaat tttgatatgt attcttcgaa atttaactaa atatacgaaa
    33601 ttcaagaagc acaataatta atcatttttc ctatacaaaa gttcgtatga ctgcattata
    33661 aaagcataaa tttataattt ttttaaatgt cattgaacgt gataatgtga atggattgag
    33721 caattttgaa aaagtgaaaa ataacctatg cggcttgcaa ttaattttca gtacgttata
    33781 atgcacactg tgcaaaatta aggaggtcta ttattcacat gatgatgaat aaagaagcaa
    33841 caaaaattgg atttgcctac gtcggcattg tagtgggcgc aggattttca actggacaag
    33901 aagttatgca atttttcact aaatatggct tgtgggctta tttaggtgtt attatatctg
    33961 gttttatttt agcttttatt gggcgccaag tagcaaaaat tggtactgcc tttgaagcga
    34021 caaatcatga atcaacatta caatacgtat tcggtgaaaa gtttagtaaa gtctttgatt
    34081 atattttaat cttcttctta tttggtatag ctgtaaccat gatagctggt gcaggcgcaa
    34141 catttgaaga aagttataac atacctacat ggctaggtgc tttaattatg acattagcga
    34201 tttatattac gttgctatta gactttaata aaatagtacg tgcactaggt atcgttacac
    34261 catttttaat tgttttagtt gtattaatcg ctggcgttta tttatttaaa ggtcatgttt
    34321 cattagcaga agttaaccaa gtagtgcctg aagcaagtat ttggaaggga atctggtttg
    34381 gtacaatata tggtggatta gctttttctg taggttttag taccatcgta gcaatcggtg
    34441 gggatactga aaagcgtaca gtgtcaggtg caggcgcgat gtatggtggt attatctata
    34501 ctgtattact agcattgatc aactttgcat tgcaaagtga atatccaact attaaaaatg
    34561 cctcaattcc tacattgacg ttagcaaata atatccatcc tttaatagca acagtgttat
    34621 ctgttattat gctggcggtt atgtataata ctattctagg actaatgtat tcatttgcag
    34681 cacgttttac agaaccatac agtaaaaatt atcatatctt tattattata atgatggtag
    34741 caggttattt attaagtttc gtaggatttg ctgaattaat taataagtta tatacaatta
    34801 tgggatatgt aggcttattt attgtagtag ctgtaattat taaatatttc aaacgtaaaa
    34861 atgcggataa aaaacatatt gcttaatatc atatgaggga tatccgaaac tttacaattg
    34921 aatcactttg ttttaacctt aaaagcaatt cgtctctact cttatcgggc gaattgcttt
    34981 ttatatttat tcagtctatt aatatgagcg tctaacaaat agagaggtac gatgtaatga
    35041 ataaagataa taaatggacg atgataactg cgctttttat aactgtaatc agtgtattgt
    35101 tagcatttca tctgaaacaa cattatgacc aaattacaaa tgagaaccat gctaataaag
    35161 acaaaattaa tattaaaaat aaaaatgtgc gcatttatca aaaccttaca tacaatagag
    35221 ttttccctaa cagtaaatta gatattatta cacctgttga tatgtcttct aatgccaaac
    35281 tgccagttat tttttggatg cacggtggtg gttatattgc gggtgataag cagtataaaa
    35341 acccattatt agcgaaaatt gctgaacaag ggtacattgt tgtgaatgta aattatgcat
    35401 tggcgccaca atataaatat cccacaccat taattcaaat gaatcaagca actcaattca
    35461 ttaaagaaaa taaaatgaat ttacctattg attttaatca agtaattatt ggcggtgatt
    35521 ctgcaggtgc tcaattagct agccaattta cggcaataca gacgaatgat cgcttaagag
    35581 aagccatgaa atttgatcag tcattcaaac catcgcaaat taaaggtgct atactatttg
    35641 gtggttttta taatatgcaa acagttagag aaactgagtt tccaagaata cagttattta
    35701 tgaaaagtta tactggcgaa gaagattggg aaaagagttt taaaaacatt tcacaaatgt
    35761 cgacagtaaa acaatcgaca aaaaattatc caccaacatt tttatctgtt ggagatagcg
    35821 atccattcga aagtcaaaat atagaattca gtaagaaatt acaagaattg aatgtaccag
    35881 tagatacttt gttttatgat ggtacgcatc atttacatca tcagtatcaa tttcacctta
    35941 ataaacctga atcgatagat aatatcaaaa aagtgttact tttcttaagt cgtaatacat
    36001 cctctagtgg tattcaaact gaagagaaac cacaaataga aaatccgagt aatgaattac
    36061 cgttaaatcc tttaaactaa tgataaacag tagtaattta ttgcttaagc aacatttaag
    36121 attttcaaat taaaaacgaa gaatttaaaa catgtggtgc taatgtgtaa gagtctgaga
    36181 tataataaat tgttagcggt tctttatcat ttctatctca ctctattatg atgtgacatt
    36241 tattttacaa actaatttgt tttgaacctg aaaataactt tttacaacta aattgtcgaa
    36301 aaacagtgtg tacatgatat aataaattta taaattgaaa agaattcaaa agaaaaatta
    36361 aaatatagat tgagcacgaa gtgatttgaa ataaggttgt gaaagggaat gacaaggtca
    36421 gcattaaaac catttaaaaa taaacgcgtt atggttactg gacgtataca acgtgttttg
    36481 tttaaaaatt atttagatag acatagcaca tttaagccga atgtaaggat attattaaaa
    36541 gatgtatttg tttcaggtgt atcaatagat catttatggt tatatgagac aaataaatac
    36601 tatgcattgg caatggaact tattcatcaa cgagtaaaat ttagtgcgaa tgttgtaccg
    36661 tattacaaaa taaatagaaa taataattta ttcgtacaag attatggaat taagcgtaaa
    36721 ggtaggttaa ttactgaaga agcttacaat caaaacaatc agtatcagga taagatatat
    36781 gaaaaattac cggatataga ttttagactc gaagattttt atagtaagga aaactaaaca
    36841 acatatataa agcaccacta atcgtagttg atagttaatc aattacacat tagtggtgtt
    36901 ttattttgaa tttaaatttg ataaatgaat aagagtgtac gtatttattt gagattagat
    36961 aacattgata agtatgagta atgtattagg taaccattaa attcgttgtt atacgattct
    37021 gatacaagat tatgataaaa tagctttaat taagaatttt agccatcata tagtcatcat
    37081 aatatttacc atcgataaat aacttatctt ttaaaacgcc ttcgatttga aaatcggcac
    37141 ttttaaaaag ctcgagggca ggttggttat tgagtggtac atttgcttca attcggtgta
    37201 tttgattgtt taaacaccaa gccataatgg catcaagaag tgcttggcca attccacgat
    37261 gttgatataa tttctttaca cctaaatcaa ttttagcaac atgtttaatg cgttgaaatg
    37321 gtgtcgtatt aacaaaggca aagccaacga gttgttcatc actttcagca acgaagatga
    37381 ctttatgcgg agaagtgata tattcttcta attgtttact agccgatgtg acgctaggat
    37441 catattctcc tggtgtgtag aacatatacg gagattcgtc gtatatgttc gctaacattg
    37501 aaatgaaatt ttctacatct ttgatactaa ctctacgtat aatatgggcc ataaaaagcc
    37561 tccagtattt tgaaatttta aaacatttgc tactattata atatatatgt aactaaaagg
    37621 tggagtaata tgttttttgg ttacagttta aaggagtatt ttagatgaaa cctaaagttt
    37681 tattagcagg tggaacagga tatattggta agtatttaag tgaagtgatt gaaaatgatg
    37741 ctgaactttt tgctatatca aaatatccag acaataaaaa aacagatgat gttgaaatga
    37801 cttggattca gtgtgatata tttcattacg aacaggttgt tgcagcaatg aatcaaatag
    37861 atattgctgt attctttatc gacccaacaa agaattctgc caaaataaca caatcatcag
    37921 caagagattt aacattaatc gcagcagata attttggtcg agcagcggct atcaatcaag
    37981 taaaaaaagt aatctacata cctgggagtc gttatgataa tgaaacaatt gaacgcctag
    38041 gtgcatatgg tacacctgta gaaacaacaa atttagtttt taaacgttct ttagttaatg
    38101 tagaattaca agtttcaaag tatgatgatg ttagatcaac gatgaaggta gttttaccaa
    38161 agggatggac attaaagaac gttgtaaacc attttattgc atggatgggt tacactaaag
    38221 gaacttttgt gaaaacagaa aaatcacatg atcaatttaa gatatatatt aagaataagg
    38281 tgcgaccgct cgcagtattt aaaatagaag aaacagctga tggaataata actttaattt
    38341 tattgagtgg aagtttagtg aaaaaatata cagttaatca agggaagtta gaatttagat
    38401 taatcaaaga gtcggcagtc gtttatatac atctatacga ttatatccct cgattatttt
    38461 ggccgattta ttactttata caagcaccaa tgcaaaaaat gatgattcat ggctttgaag
    38521 ttgactgccg gattaaagat tttcaaagtc gattaaaatc aggagaaaat atgaaatata
    38581 ctaaatgata ttgggtgata tggatgcaaa tactactagt agaagatgac aatactttgt
    38641 ttcaagaatt gaaaaaagaa ttagaacaat gggattttaa tgttgctggt attgaagatt
    38701 tcggcaaagt aatggataca tttgaaagtt ttaatcctga aattgttata ttggatgttc
    38761 aattacctaa atatgatggg ttttattggt gcagaaaaat gagagaagtt tccaacgtac
    38821 caatattatt tttatcatct cgtgataatc caatggatca agtgatgagt atggaacttg
    38881 gcgcagatga ttatatgcaa aaaccgttct ataccaatgt attaattgct aaattacaag
    38941 cgatttatcg tcgtgtctat gagtttacag ctgaagaaaa acgtacattg acttggcaag
    39001 atgctgtcgt tgatctatca aaagatagta tacaaaaagg tgatcagacg attttcctgt
    39061 ccaaaacaga aatgattata ttagaaattc ttattaccaa aaaaaatcaa atcgtttcga
    39121 gagatacaat tatcactgca ttatgggatg atgaagcatt tgttagtgat aatacgttaa
    39181 cagtaaatgt gaatcgttta cgaaaaaaat tatctgaaat tagtatggat agtgcaatcg
    39241 aaacaaaagt aggaaaagga tatatggctc atgaataatt tgaaatgggt agcttatttt
    39301 ttgaaatctc gcatgaactg gatattttgg atattgtttt taaacttcct tatgttaggc
    39361 attagtctaa tcgattatga ttttccaata gacagtttat tttatattgt ttctttgaat
    39421 ttaagtttaa caatgatttt tcttttattg acatatttta aagaagtaaa attatataag
    39481 cattttgaca aagataaaga aatagaagaa attaaacata aagatttagc ggaaacgcca
    39541 tttcaacgtc atacagttga ttatttatat cgtcaaatct cagcgcacaa agaaaaggtt
    39601 gttgagcaac agttgcaatt gaacatgcat gaacaaacca ttacagaatt tgtgcacgac
    39661 ataaaaacac ctgtgacagc tatgaaatta ttaattgatc aagaaaaaaa tcaagaaaga
    39721 aaacaagcat tactatatga atggtctcgt ataaactcga tgctagatac acagctgtat
    39781 attactagat tagaatctca acgtaaagat atgtattttg attacgtgtc acttaaacgc
    39841 atggtcattg atgaaataca attaacaaga catattagtc aggttaaagg tattggtttt
    39901 gatgttgact ttaaagtgga tgattatgtt tatacagata taaaatggtg tcgtatgatt
    39961 attagacaaa ttttgtcaaa cgcattgaaa tatagtgaga attttaatat tgaaattggg
    40021 acagaattaa atgatcaaca tgtttcgtta tatattaaag actatggcag aggtattagt
    40081 aaaaaagata tgccgcgaat atttgaacga ggatttacgt caacggctaa cagaaatgaa
    40141 acgacgtctt caggtatggg tctatattta gtaaatagtg taaaggatca attaggtatt
    40201 cacctgcaag tcacgtcgac tgttggtaag gggacaactg tcagattgat tttcccatta
    40261 caaaatgaaa ttgttgaacg catgtcggaa gtgacaaatt tgtcatttta aacatgcgtt
    40321 ttgttactta gaattgatac atcaatgcag cttcaacgtt ataataagat agatgttagt
    40381 catatgttaa attgaagata caagtgccaa agcctaaagg aaatgaagtt aagataaatt
    40441 ataggagtgt taaagtggca attttagaag taaaacaatt aacaaaaata tatggaacta
    40501 aaaaaatggc acaagaagtg ttgcgagata tcaatatgtc tattgaagaa ggcgagttta
    40561 ttgctattat gggtccctct ggatctggga aaacgacatt attaaatgtt ttaagttcaa
    40621 ttgattatat ttcacaaggt tctattacat taaaaggaaa aaaattagaa aagctttcaa
    40681 acaaggaatt atctgatata cgcaagcatg atattggttt tatttttcaa gagtataatt
    40741 tactgcatac attgactgtt aaagaaaaca taatgttacc actaacggta cagaagttag
    40801 ataaagaaca tatgttaaat cgttatgaaa aagtagcaga agcattaaat atattggata
    40861 ttagtgataa atatccctct gaattgtctg gtggacaaag gcaacgaaca tcagctgcca
    40921 gagcatttat aacattgcct tctattatat ttgctgacga accaacaggt gcattggatt
    40981 ctaaaagtac tcaagattta ttaaaacgat taacaagaat gaatgaagca tttaagtcta
    41041 caattattat ggtaacgcat gatcctgttg cagcaagcta tgcaaatcga gtagtgatgc
    41101 taaaagatgg tcaaattttc actgaattat accaagggga tgacgataaa catacctttt
    41161 tcaaagaaat aatacgtgta caaagtgttt taggtggcgt taattatgac ctttaacgag
    41221 ataatattta aaaatttccg tcaaaattta tcacattatg ccatctatct tttttcgtta
    41281 attacgagtg tagtattgta ttttagcttt gtagcattaa aatacgctca taaactaaac
    41341 atgacagagt catatccaat tataaaggaa ggctcacaag tcggaagcta ctttctattt
    41401 ttcatcataa ttgcattttt gttatatgcc aatgtgttat ttattaaacg acgaagttat
    41461 gagcttgcat tatatcaaac attaggttta tctaaattca acattattta tatactaatg
    41521 ctcgaacaat tactaatatt tataattacg gcaatattag gtattattat tggtattttt
    41581 ggttcgaaac tgttattaat gattgtcttt acattattag gaattaaaga aaaggttcca
    41641 attattttta gtttgagggc ggtatttgaa acattaatgt taatcggtgt cgcttatttt
    41701 ttaacatctg ctcaaaattt tatattagtg ttcaaacaat ctatttcaca gatgtcaaag
    41761 aataaccagg ttaaagaaac aaatcataat aaaattacat ttgaagaggt tgttttaggc
    41821 atcttaggta tagtattgat taccacagga tactatctat ctttgaacat tgttcaatat
    41881 tatgattcta tcggtacact tatgtttatt ttattgtcaa ctgtgattgg ggcatactta
    41941 ttttttaaaa gctctgtttc tctagttttt aaaatggtga agaagtttag aaaaggtgtt
    42001 ataagtgtaa atgatgtcat gttctcatca tctattatgt atcgtattaa gaaaaatgct
    42061 ttttcactta cggtcatggc aatcatttca gcgattactg tttcagttct ttgctttgct
    42121 gctataagta gagcgtcctt atcaagtgaa ataaaatata ctgcaccaca cgacgttaca
    42181 attaaagacc aacaaaaagc taatcaatta gcaagtgaat taaacaatca aaaaattcct
    42241 catttttata attataaaga agtaattcat acgaaattgt ataaagataa tttatttgat
    42301 gtaaaagcga aagaaccata caatgtaaca attactagtg ataaatacat ccctaatact
    42361 gatttgaaac gtgggcaagc tgatttattt gtagcggaag gttctatcaa agatttagtg
    42421 aaacataaga agcatggtaa ggcaattata ggaacgaaaa aacatcatgt taatattaag
    42481 ttacgtaaag atattaataa aatctatttt atgacagatg ttgatttagg tggaccaacg
    42541 tttgtcttaa atgacaaaga ctatcaagaa ataagaaagt atacaaaggc aaagcatatc
    42601 gtctctcaat ttggattcga tttgaaacat aaaaaagatg ctttagcatt agaaaaagcg
    42661 aaaaataaag ttgataaatc tattgaaaca agaagtgaag cgataagctc aatatcaagt
    42721 ttaaccggaa tattattatt tgtaacatca tttttaggta ttacattctt gattgctgta
    42781 tgttgcatta tatacataaa gcaaatagat gaaaccgaag atgagttaga gaattatagt
    42841 attttgagaa agcttggatt tacacaaaaa gatatggcaa ggggactaaa gtttaaaatt
    42901 atgtttaatt ttgggttacc tttagttatt gcactatcac atgcatattt tacatcatta
    42961 gcatatatga aattaatggg tacaacgaat caaataccgg ttttcatagt aatgggatta
    43021 tacatttgta tgtatgctgt ttttgcagtg acggcttata atcattccaa gcgaacaatt
    43081 agacattcca tataaaatat acagatggct ttcagtagag tagtggattc ggattcacga
    43141 actatactgg aagcttttta ttataaatga agagaagtta tatttttagc atgtatagtt
    43201 gaatactggg ttaaaatacc atattaataa tgaagtaaag gtatgagtga ttatgaaagt
    43261 gttttgaatg aaatatattt aattggtgat gcttttaatt gaaaagatta acaggattca
    43321 actttgtaaa ttgtattaaa tgtgagaaaa taaaagtata ttcattgaga gatatatgag
    43381 tcaatgatcg ttttaaacaa gataagtgta ttttaatatg taaaagttat gtaataaata
    43441 ttgtatcgtt gcaaatttcc caactatatc catttacaat ttttagagtt gtatagataa
    43501 aaaattgcct atacaataac tttttcaaaa caaatgccta
//
LOCUS       DALAGF010000012        40929 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.012, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000012 DALAGF010000000
VERSION     DALAGF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 40929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40929
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.012"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(408..506)
                     /locus_tag="O2692_000601"
     CDS             complement(408..506)
                     /locus_tag="O2692_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298244.1"
                     /translation="MNWIIELDLWLWCVGAINLINAVNDYANEILL"
     gene            complement(711..1307)
                     /gene="recR"
                     /locus_tag="O2692_000602"
     CDS             complement(711..1307)
                     /gene="recR"
                     /locus_tag="O2692_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCZ4298245.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            complement(1314..1631)
                     /locus_tag="O2692_000603"
     CDS             complement(1314..1631)
                     /locus_tag="O2692_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCZ4298246.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            complement(1721..3418)
                     /gene="dnaX"
                     /locus_tag="O2692_000604"
     CDS             complement(1721..3418)
                     /gene="dnaX"
                     /locus_tag="O2692_000604"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCZ4298247.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            complement(3487..4011)
                     /locus_tag="O2692_000605"
     CDS             complement(3487..4011)
                     /locus_tag="O2692_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ4298248.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            complement(4149..4250)
                     /locus_tag="O2692_000606"
     CDS             complement(4149..4250)
                     /locus_tag="O2692_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298249.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSELDVQFE"
     gene            complement(4319..4588)
                     /gene="ffs"
                     /locus_tag="O2692_000607"
     ncRNA           complement(4319..4588)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="O2692_000607"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4650..5378)
                     /gene="treR"
                     /locus_tag="O2692_000608"
     CDS             complement(4650..5378)
                     /gene="treR"
                     /locus_tag="O2692_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCZ4298250.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            complement(5403..7043)
                     /gene="treC"
                     /locus_tag="O2692_000609"
     CDS             complement(5403..7043)
                     /gene="treC"
                     /locus_tag="O2692_000609"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCZ4298251.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWYSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTIGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA"
     gene            complement(7107..8534)
                     /gene="treP"
                     /locus_tag="O2692_000610"
     CDS             complement(7107..8534)
                     /gene="treP"
                     /locus_tag="O2692_000610"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCZ4298252.1"
                     /translation="MAVKREDVKAIVTAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
     gene            complement(9005..9097)
                     /locus_tag="O2692_000611"
     tRNA            complement(9005..9097)
                     /locus_tag="O2692_000611"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9059..9061),aa:Ser,seq:tga)
     gene            complement(9366..10829)
                     /locus_tag="O2692_000612"
     CDS             complement(9366..10829)
                     /locus_tag="O2692_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HCZ4298253.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLAAAEELNLLGYQVTIYERARESGGLLMCGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            complement(10847..15346)
                     /gene="gltB"
                     /locus_tag="O2692_000613"
     CDS             complement(10847..15346)
                     /gene="gltB"
                     /locus_tag="O2692_000613"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCZ4298254.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATSGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            15527..16411
                     /locus_tag="O2692_000614"
     CDS             15527..16411
                     /locus_tag="O2692_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ4298255.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPSLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            16526..17644
                     /locus_tag="O2692_000615"
     CDS             16526..17644
                     /locus_tag="O2692_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCZ4298256.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMADGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            17641..18423
                     /locus_tag="O2692_000616"
     CDS             17641..18423
                     /locus_tag="O2692_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCZ4298257.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(18592..19077)
                     /locus_tag="O2692_000617"
     CDS             complement(18592..19077)
                     /locus_tag="O2692_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4298258.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(19067..19462)
                     /locus_tag="O2692_000618"
     CDS             complement(19067..19462)
                     /locus_tag="O2692_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCZ4298259.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEELNLTLTMDEIEYVGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            19611..19880
                     /locus_tag="O2692_000619"
     CDS             19611..19880
                     /locus_tag="O2692_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298260.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            complement(20065..21069)
                     /gene="aaa"
                     /locus_tag="O2692_000620"
     CDS             complement(20065..21069)
                     /gene="aaa"
                     /locus_tag="O2692_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HCZ4298261.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(21401..22243)
                     /gene="gmpC"
                     /locus_tag="O2692_000621"
     CDS             complement(21401..22243)
                     /gene="gmpC"
                     /locus_tag="O2692_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HCZ4298262.1"
                     /translation="MKRLIGLVIVALVLLAACGGNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDDDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            complement(22280..22939)
                     /locus_tag="O2692_000622"
     CDS             complement(22280..22939)
                     /locus_tag="O2692_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4298263.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            complement(22943..23968)
                     /locus_tag="O2692_000623"
     CDS             complement(22943..23968)
                     /locus_tag="O2692_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298264.1"
                     /translation="MIEFRQVSKTFNKKKQKIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGK
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFEDTAINQYFKEKN
                     IQFEEVH"
     gene            complement(24262..25404)
                     /locus_tag="O2692_000624"
     CDS             complement(24262..25404)
                     /locus_tag="O2692_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HCZ4298265.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIATLENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            complement(25388..26302)
                     /locus_tag="O2692_000625"
     CDS             complement(25388..26302)
                     /locus_tag="O2692_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCZ4298266.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGAEVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENNNEQEN"
     gene            complement(26519..27856)
                     /locus_tag="O2692_000626"
     CDS             complement(26519..27856)
                     /locus_tag="O2692_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HCZ4298267.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIVIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            complement(28021..28134)
                     /locus_tag="O2692_000627"
     CDS             complement(28021..28134)
                     /locus_tag="O2692_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298268.1"
                     /translation="MGIAPEVVMWRDVGDIDGVVCGEILAHVYSVEPENLM"
     gene            complement(28312..29046)
                     /locus_tag="O2692_000628"
     CDS             complement(28312..29046)
                     /locus_tag="O2692_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCZ4298269.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            complement(29083..29757)
                     /locus_tag="O2692_000629"
     CDS             complement(29083..29757)
                     /locus_tag="O2692_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCZ4298270.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISLA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            29795..30211
                     /locus_tag="O2692_000630"
     CDS             29795..30211
                     /locus_tag="O2692_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298271.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            complement(30440..30802)
                     /locus_tag="O2692_000631"
     CDS             complement(30440..30802)
                     /locus_tag="O2692_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCZ4298272.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(30964..33669)
                     /locus_tag="O2692_000632"
     CDS             complement(30964..33669)
                     /locus_tag="O2692_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HCZ4298273.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPEMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHYFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVAKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQMGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAVAEHHTSTDTLAWVYVPDT
                     LSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGTLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVIQAPDYVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            complement(33682..35166)
                     /locus_tag="O2692_000633"
     CDS             complement(33682..35166)
                     /locus_tag="O2692_000633"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HCZ4298274.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGITLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILGWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYAVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKAFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            35687..35752
                     /locus_tag="O2692_000634"
     CDS             35687..35752
                     /locus_tag="O2692_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HCZ4298275.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            35784..35849
                     /locus_tag="O2692_000635"
     CDS             35784..35849
                     /locus_tag="O2692_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HCZ4298276.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            35901..35969
                     /locus_tag="O2692_000636"
     CDS             35901..35969
                     /locus_tag="O2692_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HCZ4298277.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            36033..36095
                     /locus_tag="O2692_000637"
     CDS             36033..36095
                     /locus_tag="O2692_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HCZ4298278.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(36754..37956)
                     /locus_tag="O2692_000638"
     CDS             complement(36754..37956)
                     /locus_tag="O2692_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCZ4298279.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKMLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTELDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            38075..38335
                     /locus_tag="O2692_000639"
     CDS             38075..38335
                     /locus_tag="O2692_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298280.1"
                     /translation="MSALLYNGDKDISHERESRCINMLFVILVLYVTGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            complement(38625..38939)
                     /locus_tag="O2692_000640"
     CDS             complement(38625..38939)
                     /locus_tag="O2692_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298281.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMEAQKENIRTVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(38958..39281)
                     /locus_tag="O2692_000641"
     CDS             complement(38958..39281)
                     /locus_tag="O2692_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298282.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            complement(39278..40606)
                     /locus_tag="O2692_000642"
     CDS             complement(39278..40606)
                     /locus_tag="O2692_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298283.1"
                     /translation="MLSRKYKIDLKAINQNTESTSAISKASYEVENANNNGLSKRDVI
                     NQFNDLKKMKKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKK
                     LKEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFI
                     TGHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITR
                     FVAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYA
                     EENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQ
                     LIDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMV
                     REPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            complement(40596..>40929)
                     /gene="lpl10"
                     /locus_tag="O2692_000643"
     CDS             complement(40596..>40929)
                     /gene="lpl10"
                     /locus_tag="O2692_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl10"
                     /protein_id="HCZ4298284.1"
                     /translation="IENFKFFSQYADFKDLKNYQDGNITTNENVPSYEAQYKMNNSDK
                     NVKKLREIYPITTNNSPNLKLYIDGDIKGSSVGYKKIEYKFSKDKGQETTLRDYLNFG
                     PSEGENVE"
BASE COUNT        12824 a         8403 c         5919 g        13783 t
ORIGIN      
        1 aacatttaag tcaataactt tttaaaatta attttcgtta gtaattcaag ttatttctta
       61 agttgagttg ttttgtttgc ctcaacaaat aaatactata caggctttca aactgcttaa
      121 caacagtaaa aattacacct ttgtcatctg tactgaacgc ccttatccta aaaatcgaac
      181 ttaaaaaatc cccacttata aatagtacgt tttttagaac aagaaacaga tttttctcta
      241 accatatcaa tcaaaaaaaa cgcaaaacag aacgttattt ttttaccaga aattaaaaat
      301 aaatttacaa gttttaaata atctctcaaa acaccgctat acaggctgat ttaataagct
      361 ttagaccaaa gaagtacaac ttactaagcc agcatctatc atttcaatta caaaagaatt
      421 tcatttgcat agtcattaac cgcatttatc aaattaatag caccaacaca ccataaccat
      481 agatctaatt cgataatcca attcacatca ttactattag aagtaacaaa tcacaaccaa
      541 cggcttgctt cctaaagatt acccatccct cagtaaaact aggtaaacca taccaccgcc
      601 acaccgcaaa taaagtagta catcacacca caccataaaa aaagccacaa tgattacact
      661 gtgacttttc ttactatagt attaaaatca aaaatgttta atagaagaca ttacatttct
      721 gttctacctg cgattgcttt agataatgtt acttcgtcag catactctaa atcgccacct
      781 accgataacc cttgtgctaa tctcgtcact ttgataccta taggcttaac taatctagaa
      841 atatacatgg ctgtagattc cccctctaag ttcgggttca tagctaagat taattcgcta
      901 acttcatcgt ttttcaagcg ttcaatcaat gaaggaatat taatatcttc tggtccaatg
      961 ccatccatag gcgaaataga cccatgtaaa acgtgatata aacctttgta ttctctcatt
     1021 ttttccatag ctatgacatc tttgtcatct tccacaacac aaataactga acgatctctt
     1081 tgcttatctt cacaaatata acatggatca ttttcagtaa tgtgaccaca tacgctacaa
     1141 tatgttaatt ctctcttaac atctactaat gctttggcaa actgaacaac atcgtcttct
     1201 ttcatatcta aggtatgaaa agccagacgt tgggctgtct ttggaccaat gcctggcaat
     1261 ttcataaagc tatcaataag ttttgatata ggttctggat aatgcatcta tgatcacatt
     1321 ccagggatgt ttaagccttg agtatgttta cctaaacgtt cttgagtaag ctcatcagct
     1381 ttattcatcg cttcattagt agctgctaac actaagtctt gtagcatttc aatatcgtct
     1441 gggtctacag cttcttcttt gatttcaacg tcgacaactt ctttatgacc agttacagta
     1501 actgcaacca tgccaccgcc agctgttcct acaatacgct cttctttaag tttttcttgt
     1561 tcttgagcca ttttcttttg cattttttgc atttgtttca tcatttgttg catgtttccg
     1621 ccaccgcgca tattccattt cctccttgaa aatcaataaa tatttatcaa taaaatgatg
     1681 tttctttcat tatacatacg attatatcgc ttgtcatgta tcactcttca tctatcacat
     1741 gtacagtttc ttcaccgaaa agatcttttg ctttttgagc aatatctgtt tgttgtgctt
     1801 gttgctttgg catatcatcg ccttcgtttt tacgattttg taaatattcc gttcgaactc
     1861 tttgccattg atctgatggt acaccaacaa ctttaacgtt tttattaacg atattacata
     1921 caacactttc tatactacta cgtttctcgt cgtctttatt gacgatttca caatggatct
     1981 cttcctcaaa tttcacaagt acgtgatctt cacttgccgc cacaggttcc gaattttgca
     2041 ataaactaac gagtgatttt ttatcattat ttttggcatg atcaatcact tcttgccaat
     2101 gatctttcaa caatttgata tctgccttat tcgctttatc tagcactttt gcaatttgtt
     2161 gcattgaaaa tgcattttta gatttttgta tgcctctcgc aggctttttc gaagattttt
     2221 gaacaggagc gacactcact ccttgtgctt ttagtgtttt tagttcttgc tctaactgtt
     2281 ccatacgttg caacaataca tctgtgtttg gcgatgaagc aatttgtgct ggttcagcta
     2341 cattcgcaat cacttgtggt tgacccttaa tctgctcagc taattttact aacaacactt
     2401 caaaatgaac gttttgattc acactaaaac gaatcgacac taatgtatca ttaataagat
     2461 caatcatttg atataacata tctaattcta agttcatcag tgctcgatac tcagtatctt
     2521 tctcagatgt tttattcata atcgtatctc tgacaaaata aatcatatca tttattaggc
     2581 gattcacttc tttaccttct gttataaact gatggtattt tttaaaagat gcttgtacgt
     2641 caccttgtac aatatcatca aacaagtgat ccaacgcttc atcatgtacg ctacctgtga
     2701 cattcaacgc atcttgcaat gttaacgtac catcaccaaa tgcaatagcc tgatccataa
     2761 tacttaatgc atcacgcata cccccttcag acgctttagc gataaatgcc aaggcttcat
     2821 cttcacattc aatttgttgt gcatctgcta caaattttaa acgttcaaca atttgatcta
     2881 ggctaattgc tttaaaatca aaacgttgtg ccctagaaat gattgttgga gggattttat
     2941 gtggttctgt cgttgccaat ataaaaatag cgtgtgctgg aggttcttct aacgtcttta
     3001 aaagggcatt aaaagcacct gttgttagca tgtgcacctc atctataata taaactttat
     3061 atttcgattc acttggtgca tatttaactt tgtctctaat atttcttatt tcatcaaccc
     3121 cattattact agcagcatca atttctatca catctgaatt agtcccctgc gtaatgcctt
     3181 tacaaatatg acattcatta caaggttctc catcagtgct atttagacag ttgattgctt
     3241 tagcaaacac tttggcaata ctcgttttcc ccgtacctct cggaccacta aaaatataag
     3301 catgcgactg tttttctttc gaaatcgcat tgcgcaatgt cttcgtgaca tgttcttgtc
     3361 cgacgacatc ctcgaaactt tggggtctgt acatacgata taaggcttga taattcaagt
     3421 tagcacctcc ataaacaatt acctctcatt atagcatgat aataccttta cttcttaatt
     3481 gaacaattaa taaaaatgtt ctggaaattt tacgattccg tgtggtgcat ccgtcaaatt
     3541 atcccataaa tattttacaa gtaacggtgc atcaccagat tctaatttaa tgtcatgctc
     3601 agcagcattt tgataaccca acttttcaaa gtagtcaaaa caatggtcta caacaaccgt
     3661 actatactct tgtgctttgg cacgctcttc tactgcttga accaagccac gacctaattt
     3721 ttgtccacgt aattcaggat gaactgataa agaggcaatc gccaaaccat aatacgtctt
     3781 atcatcacta ttaatttcta cttcaattaa taaaacgtgt ccaacgacat cgttattttc
     3841 atttttcgct attacttcta attcaaaatt atagcaagga gattttctta aatgttttac
     3901 tttcgcacgt gcttgccaac tcgtttcagg attatcatca aaactttctt caatactatt
     3961 taaagattta tcataatcta actctgttaa agtacttaaa tatatttgca tatgtcctcc
     4021 gtaggcattt gattgtcaat aatcataacg tatctcatta ataattctat tgtaagatac
     4081 tccccctcat ttcaccatca tttctttatc atcaaattat aacttcttac ttttcattga
     4141 cacaaaaatc attcaaactg cacatcgagt tcacttgaat caaacttcac atataaaaaa
     4201 gctacttccc acaaacatgt tccacgtata atacgctgaa ttgtcttcaa gaaagtagct
     4261 tctataatta tatattttca actcttaata atcggtttaa tatttaaaaa ataaaaaacc
     4321 gtgcacctaa gcatcgaacg taattcacga acgtaaccaa agtcgttagc taaatctcgg
     4381 ctaccctacg gcacatatga tgatccactt aatgctgctt ccgtcaggac ctgacatggt
     4441 tcatgggttc atattgcata ggaccgaaat cttcaaacac tacgtgcttt gggcagactt
     4501 cgcaaaaata cggcctcaac aaaggaatta agcctcgcat aaagcggatt tcgagtacag
     4561 ggaaccgcta cctccccacc tagcacggca agatatatat tactatattt taatagttaa
     4621 ttgcaagtat aaatcattta tatcattgtt tactttatac gacgtcttga gaagtcatta
     4681 aatttaaatt catttgcaag atgttttgaa atattatatt gaaacggcat tgtattttct
     4741 aaatacacaa tacttcgaac tgttgctgaa taagccaccg atacatcacc aaacaattga
     4801 tatgcttgtt catcaaacgg ttcaaaagta atagacttac ttgaataact aatattaaga
     4861 tttaatacct tttccaaata atcataaata gaatcactcg caacatcatt acctatatct
     4921 gaaacaatcg actttagaaa ataatcttca tcaacaatct tcacatgttg gttaagccga
     4981 cgagttctaa caatatgaat gagtgattca ctagaattga tgtttaaagc atgttgaact
     5041 tctggaactt catgcgcttc aacaacctca ttcacaacaa cttcagttaa atatgcgacg
     5101 cccatttctt cttgcatttc tttaaaacta acaagttcag aaaatggaaa ctctgtaacc
     5161 tcctgataaa tgacaagtga ccctttacca tgaatctttt gaatcatgcc gtctaatgcc
     5221 aacaaatcta atgccttacg cacggtctct cgagatgact ggtacaattg caccaaatca
     5281 tgttcagacg gaatttgttc accatattga atctgcccgt ttaatatatc ttctttcaac
     5341 gcctcataaa tcttcataaa ctttttttgt ttcgccatat aaacgcatcc actcaattta
     5401 ttttacgcgt ttgtttcgat tacaattgca ccaaacccgc ttatcgtatt attttccact
     5461 gtgcctgttt gaatcacaac acaaccttct ctagccaatc cttctggcaa atcaacagcc
     5521 gatgctgaga aattcgcaat tactagccat tgttgattct tataatgacg ttcataaaca
     5581 aataaatgat catgatccat aaaacgtggc acaatgtctc catacgtaat aatatcatgc
     5641 gtatgtctta attgtattaa tttttgatac gtatagaaaa tagactcttt attctgaagt
     5701 gcttgtctaa cgttgacctg atgataattt tccgaaatat caatccaagg cttaccagct
     5761 gtaaatccag cattaacatc atcactccat tgcataggcg ttctcgaatt gtctcgtgac
     5821 ttctgtccta aaatcgctaa cacatccgct tcagcatgcc cttcacttaa caactgatgg
     5881 taggcattaa tcgattcaac atcacgatat tgtgctattg atgtaaaatg tgggtccgtc
     5941 ataccaattt cttcaccttg gtaaatatat ggcgtccctt gcaacatatg cagtgcgata
     6001 gctaacattt tagcactttg tatcctcatc tcttccgacg tatcatcacc aaatctagac
     6061 actacccgtg gctgatcatg attacaccag aaaatcgcgt tccatccgcc accgtcataa
     6121 atacctcgtt gccattgcat cagaatttcc tttaacttat gaaaatcaag cttcgcattt
     6181 gtccactttt caccatcaac ataatcaacc tttagatgat gaaaattaaa aacactattc
     6241 aattcttggc gttctggttg tgtatactta atacaatttt caatcgtcgt cgaagacatt
     6301 tctcctatag tcatcatgtc agtgttacca aacgtttgac gatttaattc atgcagaaac
     6361 tcatgcactc taggaccatc cgtataaaat tctttaccta ttttgtcaga gtccttaaat
     6421 tcacctttag aaattaagtt aatgacatca aatcgaaaac catcaacgcc gaagtctatc
     6481 caatgattga ctatgcgata taacgattga cgtacttccg gattatccca atttaaatca
     6541 gcttgactga catcaaataa atgtaaataa tattcatctg tctcagaatc atacttccat
     6601 gcattaccac cgaatttaga ataccaattt gttggcggcc cgtcttcaga tgatctgaaa
     6661 aagtaataat ctctataagg gttatcttta gatttacggg cttctttaaa ccattcatgc
     6721 tccgtcgacg tatgattaat gacaatatct aacatcactt tcaagtcttt ttgatgcgca
     6781 actttgatta acttttcaaa atcatccatc gttccaaagt cttcattgat ttctaaataa
     6841 ttgctgatat catagccatt atcattcatc ggtgattcat acactggtgt taaccaaata
     6901 taatcaacac ccaataactt gatataatcc aatttttcta taattccatt gatatctcct
     6961 ataccattcc ccgtcgtatc attaaacgac ttaggataaa tttgatatac aacggatttt
     7021 ctccaatcta tttctttcga cacacaatta ccaccttata acgtaataaa ggacgtccaa
     7081 ataacgaacg cccctttttt attttattaa tcttcaacaa tttctttcgc tttttgttta
     7141 ctaaaatgag acatcacaat tgttagtata catggtacaa caatagcaat agctgtcaca
     7201 ataagatata ctggccaaaa ttctttttga attgaaatga atgctggcac gccaccaaca
     7261 ccaacttttc caagtacgtt attcatacca acgattgccc ccaatacaca agacgttgat
     7321 atcgcagcga taaatggata tttcagaggt aagttcacac cgaacatggc tggttcagta
     7381 acacctaaca taccagaaat acaagatgtt aatgccaagc cttcttcttt aaccatttta
     7441 cgacgtttat agacaaacca tgctccaaat gctgcagagc cctgacaaat attggaaatc
     7501 gcaacaattg gccataaata cgtaccgcct aagctgctac ccatcaattg gaaatctact
     7561 gctaaaaaca tatggtgtag tcctgtaatt acaagtggtg catataacaa tccatatatt
     7621 gctccgccaa gccatcctgc atgttggaat ataaatgtaa caccagatgt aatacctgta
     7681 ccaatcaata acgcaactgg tccaatgata ataaatgcta aaaatccagt aactaaaagc
     7741 gctacgggtc caacgaccaa catttttatc gaatcgtgaa cgactttatt taatcctttt
     7801 tcaattttag ctagaacgta agctgcaatt aaaactggca acacttgacc ttggtaattc
     7861 aactgcttaa tctctaagcc aaataagttc cacgtcggaa tattcccttt tgccaaatca
     7921 tactgagata ctaattgcgg atgcattaaa atcaaaccta agactatgcc tagaatcgga
     7981 ctaccaccaa atacacgcat actactccaa ccaattaatg ctggtaagaa aataaatgcc
     8041 gtactcgcaa tcacattaat gatgtttgaa atatcagcaa tttgtggata catctcaata
     8101 agtgcttttg gaccaaataa acctttcatt gtaagtaaat tattgattcc cattaacaaa
     8161 ccagctgtca caatcgcagg taatattggt ataaaaatat cccccaacaa tttgatcaaa
     8221 cgttgtactg gattcccttt ttgtgcagct gcttgtttcg cttcatcttt cgaagcttct
     8281 tgagcacctg tttcatcaat aaactgctta tacacttcat cgactgttcc tggaccaatg
     8341 acaatttgat attgatggtc tgctttaaac tgccccttga ccaacgcgtt attacttaat
     8401 gcgtctttat caactttact ttcatccttc agcactaaac gtaatcgtgt tacacaatgc
     8461 gttgcagctt caagattttc ttttccccca atagcggtta cgatggcttt tacatcttct
     8521 ctttttacag ccatatattt cactccctta atgtcttaaa acgagtataa cttgtctaga
     8581 caagttatgt aaaggctttc aacaaaatat ttttattttg tcacaattat ctggggtagc
     8641 catttcagaa aatcaatgag ccataacgac atatgtagca ataactcctc gctacaattg
     8701 cgttattaac aatacctcac cacgctctca ccattataaa gtgtagatgc atggccaact
     8761 cctaaattga aaaatgcata tctataaata gcaaacttgt ccacacaagc catccatcat
     8821 atttcacaaa acaaaaaaga catttagcta tgtatattgc acaagccaaa tgcccttcca
     8881 atctcaatgc cttatcattt ctattatcaa aaattacgag ccattcaaag acaaataaaa
     8941 aaggtaaata ctcaaaattt gagcacttac cttcattata tatagaattt aatataaata
     9001 ttgatggcgg aggaagaggg attcgaaccc ccgcgagccg ttaagcccct gtcggttttc
     9061 aagaccgatc ccttcagccg gacttgggta ttcctcctaa acacaatatt tatcatatat
     9121 gaacgttctg taaaagtcaa tattgttttt aagcattaca tgattaccac aaatcattgt
     9181 cttccactac agttgaccta taaacgaatg taaaaaatca ctacttataa ttaatctatt
     9241 tgttgtctta caaacctttg tttttaggtt acaaagatgg catgtgacgt tgatagatac
     9301 tagatttttt caaactcgat tcagcatgtc acaacgtcaa cgtaaccacc atttccatac
     9361 aaagattata cacaaacttt actagctaaa tactgatcta ctgctttcgc tacgcctcta
     9421 ccttctttaa ttgcccatac aactaaactt tgaccacgtc tagcatctcc agcagcaaat
     9481 accttttcat tattagtttg atagtttgta tcatccgcca cgattcgatt tctatccgtt
     9541 ttaatgttaa aagcattcgg tactgttggt tctgtacctt cgaagccgat tgataataat
     9601 acaaggtcag caggccaaaa tctttcaggt ccttctttca tgaccatacc attttcgcct
     9661 tgctctaaaa tttgagtata caaaccacgt atgtgtcctt tatcgtcaac atcgtaacgc
     9721 attgtttgaa caccatatgc acgtggttcc ttaccaaact tagcttcgta ctcttggtgc
     9781 gcatagtcca ttttaaacac cggcattgct aaaggccatg atgcattttc tgtaaatgta
     9841 attgcttctg gcaatttcgt atatttatta aattgaacaa tcgatttaca attttctctt
     9901 aatgctgtcg ctacacagtc tgcccctgta tcaccagcac caatgataat gacattctta
     9961 tcttttgcag ttattgttat atcatcaatt tctccattta acaactgcgt ttgttcagtt
    10021 aaataatcca tagcgaaatg tataccatca cccatgcgtc cttctaaagg taaatctcta
    10081 cctttttgtg caccagtaca taatataatg gcatcatact cagattctaa cgttgcttta
    10141 tcaatatcaa cgccgacttc aacaccatta atgaaagtaa tgcccgcttc ttccattaac
    10201 ttaatacgac gtcgaaccac atctttatca agtttcatat tcggaatacc acacattaat
    10261 aaaccgcctg attctctagc acgttcataa atagttactt gatatcctag tagattaagt
    10321 tcttcagcag cagctaatcc tgctggaccg cttccaacga ttgccacttt ttcatctcta
    10381 cggcgactcg gaactttcgg cgctacccaa ccattttcaa aagcttcatc aataattgtg
    10441 cgttcaatac ctttaatcgc aatcgattct ctattaatct tcatcacaca agcactttcg
    10501 catggtgcag gacatacacg ccctgtaaag tcaggaaagt tatttgtttc gcttaagcgt
    10561 tcataagcag ttttaaaatc ttgatgatac actaagtcgt tccattcagg aatgtagttt
    10621 ccaattggac aacctattgt ttccctacca tactgttgtc cggtttgaca aaacggcgtt
    10681 ccacaatcca tacatcgtgc accttggata gaggcatctt ctttagtaaa tcgttgttga
    10741 tatgctttat gatgcttcaa acggtctacc agtgataatt cacctaagta ctgtttgtca
    10801 tacttcataa atcctttaaa ttcacccatc gtatctcccc ctttccttaa tacacaacgg
    10861 ctggtttatg tttagcatcg attgttttac tgtcatcgta aaatgcagct aacatcgctt
    10921 catcttcatt gtcatgtaat gatttgtgca aatgaatttt ttgcatcatt aattgataat
    10981 ctttaggaat aactttaacg acgacatctt caatgcgatc aaaatgtttt aacacatgaa
    11041 tcgctctcgt actattcgtg tgtgacacat gttcttccag catttgctta atgaatgctt
    11101 tttcttcttg gtgtttaatc tttgtaaacg aaagcgtatc tagttgatta ttttcaacaa
    11161 aagcttctac atcagacggg ataacgtaag caataccacc actcatacct tgaccgaagt
    11221 tcttacctac atcacctaaa ttaatgacat gtccaccagt catatactct aatccatggt
    11281 cgccgatacc ttcaacgaca acatctacac cactatttct aatacagaat ctttctcctg
    11341 cactaccgtt aataaatgcc ttaccacttg tcgcaccata gaatgagacg ttaccagcaa
    11401 taatttcatt ttgtcgttct tcaaaaggtg ctttgacaat gaccgtacca ccagataatc
    11461 ctttaccaac atagtcattc gcatctccag tatgatgaat cattaagcct ttcggtgcat
    11521 atgctgcaag actttgacca gcatgaccat tcgtataaac attaattgta ttttcaggaa
    11581 gtcctgcttc tccatattgt ttcgaaatct cactacctgt aataacccct acatcacgtt
    11641 gttcattatt tactgtaaag ctacctgtat agcgacgccc ttcagcaata tatggcttcg
    11701 ttacttcata taaatttgtt aaatcaaatc catgctcaag attatgattt tgttgaattt
    11761 cttttgtgtt tggcccatcg aaaggacata acagtttttc aacatcaata ctagccgctt
    11821 tgctattcgc ttttaatgtt gatgatcgtt gtaataaatc agttcttcca actaagtctt
    11881 ctacacgttt caaacctaaa gatgctaaaa tttctcttaa ttcttgtgca ataaaatgca
    11941 taaaattaac aacatgatgt gctttacctc tatataaagc acgtaaatct ttgttttgag
    12001 ttgcaactcc tactggacat gtatctttat ggcatacacg catcataata cagcccaaca
    12061 ccactaatgg tgcagttgca aatccaaatt cttccgctcc aagcgcacat gcgtacgcta
    12121 catctttacc agttaataac ttaccgtctg tttctaactt aacacgactt cttaagtcat
    12181 ttagttttaa tgtttgatgt gtttctgcta aaccaatctc ccaaggaaca ccggcatgct
    12241 gaatactcgt tttgggtgaa gcccctgtac caccatcgta accactgatg acaattttat
    12301 ctgcaaatgc ttttgccacc ccagatgcaa tggtaccaac acctgttttc gaaactaatt
    12361 ttaccgcgat atctgcatct ttattcgcat ttttcaaatc atgtatcagt tgcgctaaat
    12421 cttctattga ataaatatca tgatgtggcg gtggtgaaat cagaccgata cctggcgttg
    12481 accctcttgt cttcgcaatc cacggatata ccttagtacc aggtaattga ccaccttcac
    12541 caggctttgc accttgcgca actttaattt gaatttcttt ggcatgttgt aaataatcac
    12601 tagttacacc aaaacgccca gaagcaactt gtttaatcgc acttactttg ttgcttccat
    12661 caacttgtac ttcataacgt tttgcatctt cgccaccttc accactatta ctctttccac
    12721 ctaattggtt catggcttgt gctaacgttt catgtgcttc cgctgaaatc gatccataac
    12781 tcatcgcccc tgtattaaag cgtttgacaa tgtcacttac cggttcaact tggtcgatgt
    12841 caatcggtgt acatgcttta aattcaagta aatgtctaat gtgatctgtt ctatttttgt
    12901 tcaccgcttc agagtatgct ttaaattgcg catagtcatt ttctttacat gcgtgctgca
    12961 ataagaaaat agattccgga ttaaaagcat gatgttgacc ttgttgtctc cattggaatg
    13021 tactacctga tgcaagataa ttatcatcac tttgttgacg tgctttattt tcagcatcaa
    13081 tttgatcaat cgaaatacca gataacttag actgtgtccc agtaaaataa cgatcaatca
    13141 catcatgaga caagccaatc gcttcaaata tttgtgcccc ttgataactt tgcactgtcg
    13201 aaattcccat cttagccatt actttaatga caccttctga caatacatcc gtatatgtct
    13261 taacattatc gacaacggtg ccttgtaacc cttctgtcaa tgtcagttgt tcaactgtac
    13321 gttgcgctag gtatggcaca attgcattcg cgccatatgc gagtaaacaa gcaacatgat
    13381 gcacttctcg tgtctcacca gatttagcga ctaaacttgt agacatacgt aaatctgctt
    13441 taataagtaa ttgatgcaca tgacttattg cgagtaacat cggcattgca aagccattgc
    13501 tatcaactaa tccactatca tctaacacta gaatttgagc gccttgcttt acagcattca
    13561 ctgcttctcg gcctaatgct tctaacgcat cttccaaatc cccttcatat accgttgata
    13621 aataagttaa tttaaaatgt tcctgatcaa tcgctgctaa gtgtgattca ttcaataccg
    13681 gccttttcaa ttgaatacga tctaaaaccg tttcgtcagg tgctagtaag ttaccttcgc
    13741 cacctaaata agaaagttca ctcgttacga ttttttcacg atacgcatca attggtggat
    13801 tcgtaacttg tgcaaacagc tgtttaaagt aattaaatag tgattctggt cgctcgttca
    13861 acactgcaat tggcgcatca tatcccattg caccgatagg atccttctta ccttctacaa
    13921 gttcctgaat atacttatga atctcttctt tcgtgtatgc aaactgacgt tgtaatttaa
    13981 ataacgtctc atctttccat tgcgaatctt gatattgtat attttcaaaa tcaaagtcaa
    14041 ctttatggtt atcaatccac gctttatatg gtaattctcc agcaatcgca ccttttaaat
    14101 cattattttc aatgacttta ttctgtttaa aatcaacaag caataacttt ccaggattca
    14161 attgaccttt aaaagcaaca ttactttcag gtacgtccac aacacccact tcagatgaaa
    14221 agacaataaa gttatcttta gtaatcgtat aacgacctgg acgtaatcca tttctatctg
    14281 taagcgcgcc aagtttgtca ccgttacaga acgaaatcat tgtaggacca tcccacggtt
    14341 ccattaaata actataaaat tcataaaacg cacgtacatt tgcatcattc gcttcattat
    14401 ataaccaagg ttcaggtatg agtaacatcg ctgccttttc tggctccatg gctaacgata
    14461 agaactctag cgcattatct acaatggcag agtcactacc atcctcatcg acaatttgaa
    14521 acactttatg ttgatcctcg ccaaataatg tttcgattaa tttatgttgg cgtgctcgca
    14581 tccagtttac attaccttta atcgtgttaa tctcaccatt atgcattaac atacggttag
    14641 gatgtgccct tttccaactc gggaatgtat tcgtactaaa tctcgaatgc actaacccta
    14701 gctttgattg atataaatca tccgataaat ctgtatatag ttttttaatt tggtctgatc
    14761 gtaaccaacc tttatataca attgttttgc gtgataagct cgtaaaatac aattctaaat
    14821 cgcactgagt cgaatagaac tctaattgtt ttctcgctaa aaacaaacgc ttttcaacat
    14881 cttcaatgtc cctaatatca ataaacactt gttgaatgac tggcatcgta tctgctacat
    14941 gtttagcaat ggcatcttta ttaactggta cattacgata accaagaatt gataaccctt
    15001 cgccttcaaa atatttttta aaaactactt catgttcaga acctaaaatg cgttctttgg
    15061 aaaaaaataa ccccacggca tattcacctt cacctgggat atcaaagtcc gttacatgtt
    15121 gtttgaaaaa tgcaaaaggt atttcagtca taatacctgc gccatcacca gtgatgccat
    15181 ctgcgccgac cccgcccctg tgatctaagc gtcgcaacat ttcaagcgat ttatcaatga
    15241 tgtcgtgaga ccttttatta tccatattcg cataaaaacc aataccacac gcatcatgtt
    15301 cctcacgata gtcatataag cctttaatta atttctcatt gtgcatgatg cacacccctt
    15361 ttttagaata ttctgatatt agttattaat gataattact ataatctaaa aaatttatct
    15421 tttcaatata taaaatagaa gatattcatc taaaaaatag aacatttatc aataatattg
    15481 ctataatagt tacaaaacac tcatttttgg aggtgcctat atcgctatgg aaattaaaca
    15541 attaagatat tttatcgagg tcgctaaaag agaacatatc tccgaaacag cacttgaact
    15601 aaacattgct caatcagcga taagccgtca aattacatta ttagaacaag aacttaatgt
    15661 gtctttattt aaaaagcaag gacgcaatat tacgctcaca tctgaaggga aattattatt
    15721 taatgaagct ctgcgtatta ttgaacattt agatagcacg attgaacaat ttcaaagcca
    15781 tggattaact aaaaataaaa gtatctacat tggctatgat gagagtgacg tatcccacat
    15841 gttactacca cttatccaaa cgtttcattt acaaaatgat acacatgtca taccgagctt
    15901 attagaacat gacacaatta taaatagcgt attaaatggg aatatagaca ttggtttcac
    15961 ggaacttaca ccagaaatac gtaaacacaa acaattacat atgctacctt tattcgaaga
    16021 gcactatcat ttatatgcgc cttcagacga tccaattacg atggcgacac acccaccatt
    16081 aattcagttt gagcatagtc atatttattg cttggcacca tttgccgaaa ctgtaaaaaa
    16141 acaattgcgc aagattacta aatcagatgt atacacaatc agtagccaac cacttgcgca
    16201 gtatctcctt cgtcaaaaag aaggttacat catttcatca caaaatatac atttaccaga
    16261 atctaaatca tggatagata taaaattaga tcatactgaa ttaaaaagaa cgatatgtgc
    16321 tattacaaag gagccttata caaagagtga tattggcata ttattaacac ttattcagca
    16381 attaatgact aaaacgtcta cattccatta agtaattcga tgctatcaat gatgattttc
    16441 gttttacact tagcagtaat atctttgaaa ataaaaaatt aagggcgtat aatcaccata
    16501 ttaattcatt catagaattg gggttttgtt tttgaaatat agaaatttat ttacaaagcc
    16561 atttaatata attttaggtt tgttctgcat cgcattcatt ggtgctattt tatttacatt
    16621 caataatgag aacttttaca acaaaccaat tggtcaaatt atagatgtca aacatgtttc
    16681 ttcgacacct acaaaagatg cacaaaataa tcgtgatatt aaatataaaa atcaattaaa
    16741 agtcaaaata ttaaatggtc aatttgctgg agaaacgacg acgattaatc atcagtacgt
    16801 caaatcgcaa gcagattctg aggcatttcg cacacacgaa aaagtattat tacatatttc
    16861 taacaagccg agtgatgctt atattataga aaagaagcgt gataccttaa cggttatcat
    16921 cactggtttg ttcttattaa ctgtattatt ggttggtcgc aaagtcggac tgcaatcgat
    16981 tttatcgtta atcttgaatt ctattgccat attaatcgca atttacattc atattcaaca
    17041 tagcaatatt aatttattct tactcatgac cattgccatg atctgttcaa caatactcac
    17101 tttattgctc gttacaggct ggcatatgcg tacactcatt accatagcca gtacaattat
    17161 cggaactttt ttgagtatag gtttaacaga attaatcata tatatggcag atggtaaagg
    17221 cattaaatat gaaacgatga actttttatc attaccaccg aaagatatct tcctagcctc
    17281 agtgttaata ggttcacttg gcgctattat ggatgttgca attacaattg ctagtggtat
    17341 gcatgaaatt ttacagcgta cgccacacat ttcaatgagg agatgggcgc ttgctgggcg
    17401 aaacatcggc caagatatta tgggaacaat gactaacatc ttattattct catatttatc
    17461 cggtgcacta cctatgtttt taatcttttt aaaaaatgcc aatacagtga cttatactat
    17521 ttcgatgaat tggtcgttag aaattgcgcg cgcactaaca ggtggtatag gtatcgtact
    17581 tacaattcct atcacaatat tgtttatgga aatatttgaa acattaagga gggcgaaaca
    17641 atgaatgctg tatttatatt ggcgttattg ctcctctttc tgatgataat tttcggaggt
    17701 aaaaagggat tcatctctta tttaacccta tttttaaatt tcgctatttt aattattagt
    17761 attgtattga ttatttttgg cgtaccgatt tatgtcgtca cattcttttt ctgcattatt
    17821 attggcgctt gtaatttatt cgtactcaat agttacaatg ttaaaacaca agctgcattt
    17881 atcagcacta tcgttacaac aatattgttg attacactca tttatttatc ggtctacttc
    17941 ggtcatctac aaggcttttc aaccgaacaa caagatgaaa catatgtgta ttcaatgaat
    18001 attggcattg atatggttca atttatggtg ttcactatta ttcttgctgt tattgcagct
    18061 gtgatagatt taacaattac cattagttca cccatttatg aattgcatca agtgaatcca
    18121 acgcttacgc aatacgaatt attccaatct ggcatgcgtg ttggtagaga aattttagcg
    18181 acatctgcga acacgattta ccttgccttt tttggtggcc aacttgcttt atttatttgg
    18241 tttttcaaac ttaaatattc atttggtcat attataaact cgaaaatatt tgcccaagaa
    18301 tttatttcta ttattttagg cggtattgcc gttgcaatca gtattccgat tacggcttgg
    18361 attacggcat ttttaataaa aagaccatct cgacagcgca aaaatgatga gtctatcaat
    18421 taatcgcaac tagcaatata acttaagtta agcgacctgt ggatatatgt caattgttca
    18481 ctaggtcgct ttttactata gtaaattgtc cgatgatcta taacaaaaag cacctttatt
    18541 catcattaat tgttgtggtc gacgatgagt aaagatgctt ttaaatttaa tttattttat
    18601 agttaacgcc ataatatgaa caggtcttcc gacgaacata ctatctccca tatattgata
    18661 tccttgttgg cgataaagtg ccatggcatg tggattgtca gtattaaccg ttaacacaat
    18721 ctcattaata tcctgaaatg ttgaagtgat aaatgacgcc aatttttcca ttaccacttt
    18781 acctattcct ctattacgat aacgttgatc aacactaaat gacctgaaaa atactgcatc
    18841 ttggttatcg ctaaatggtg cgaccccttt tccacgatgt aatgtaaaaa aggccacaca
    18901 ttgcctgcca tccattacaa cgatgggata cctttcatta tcagacattg cagctgtaat
    18961 attttcctct ggcgttttaa caaacttcaa atcactttca cttaattgaa aagcttctaa
    19021 catcgaataa tcttgtggga cataatgtcg taacatgatg gtgtcattat ttgtcatgtt
    19081 taccaccata agtttcaatc caaactttga cagcaggcgc aatcaacgca tcattatctt
    19141 tatcaatcca gcgtatatcc gtaatttcat tattgatagt tacgttttcc caatcgattt
    19201 tggttaatgc gcgaaatcca tttaactcag ttaacatatc ctgttgtgga tatgcaggac
    19261 ctacaattgt cccgacatat tcaatttcat ccattgttaa tgttaaattt aattcttctt
    19321 ttacttctct taacagcgcg tgtacttgtg attccccttc ttctatttta ccacccggga
    19381 aataatactt ttcgcgattc cttacttgaa caagcaatat tttatcagct gtttcttcaa
    19441 ctaaacagac acatttaatc atctttgaca ccccaacttt gtgaaatcaa tttttcaaat
    19501 tatactgtac aattatgtta tcatatatga gtagttatag cgcaaaacgt tagcaattca
    19561 gcgcacccaa cttttcatat aaacagaaga tactaggggg aattattatt atggcaaaac
    19621 gttccaaatc acaacgttta tcaagtttac taaatgtcgc aggtttcata gtcgacggct
    19681 acaatggcta taaatatcat gctaaaaata aaaaattagt atatctttca ttaggtttaa
    19741 gcactgtagg aaccgtgtta gacttttaca tttcaattaa gtcaccacgt aagttcaaaa
    19801 aagcagtggc agttgttact ttaataacaa acggtgctag attatttaca agcattcgca
    19861 aagtaaaaca tgaatactaa ttcagaaaag gattggtcga acatagaaca tgaagttcat
    19921 tcgaccaatc ctttttcata tacaaaaatt cctattacaa caatcacgac tttgatagcc
    19981 ccgccataaa atttaggatt caatccaact tttcagcttg tgaaatgtaa taggaattca
    20041 ttatatattt atatacgtaa gactttagtg aatatatcta taattattta cttggtaagc
    20101 tggtaccgtt ctgtaagtta aaatacctgg tgcagctgaa tagttcattt ctgaaactaa
    20161 aatactacca tcgttattta cacgttctac aaacataaca tgaccatagt aacctacatc
    20221 tgtttgagcg atagaaccta cagtaggtct attgtcgata gtgtaaccat ctgctgccgc
    20281 tgcgttatcc cagttattag cattccacca ataagtacta atacctttac caatttcagc
    20341 acgacgatta aatacatgat atgtacattg accccatgta tataagtttt ggtgactgaa
    20401 tactggtgta ttgtaacctc tatttgtcgt tgttgcagat cctgagttcg tagatgcatt
    20461 accagttact ttcaattttt gacccggata tataaagaaa ttatttaagc catttaagct
    20521 cataattttt tgataagttg taccatattt tgatgcaatt aatgacaatg agtcacctgc
    20581 ttgtactgta tagtatgatc cgccacctga gttcgttgat ggacggctac tattgctcgc
    20641 agcgttactt gagctagcag tacctgatac ttttaatttt tgacctggat aaataaagaa
    20701 attatttaaa ccattaagtc gcataatgtt ttggtaagtt gtaccatatt ttgatgcgat
    20761 taatgataat gagtcgcctg cttgtactgt gtagtatgat ccgccacctg agttcgttga
    20821 tggacggcta ctattactcg tagaattact tgagccagat acttttagta cttggtttgg
    20881 gaaaattaga ttagatgtta aattgtttaa tgactttaat ttagcaatcg aaatcccata
    20941 cttatttgaa attgcccaca ctgattcacc cggttttact gtgtgagttg tagccgcatt
    21001 tgcttgagtt gccgcaacag cgctaatcgc gcttgtccca ataatagctg caattacttt
    21061 tttttgcact ttaaaatcct cctcttgctt aactttccta acattctttt atccgaattt
    21121 atgaatacta catcattata cgattttatt atgtataata ggttgatgtt tgatgacatt
    21181 atgattacaa aaaaatcata tactgtatca tcaaatttta taattacccc ttaaaattat
    21241 tacaacttat tagattttac aatatctaaa ttattacaat ttcataatat ttcactataa
    21301 aatgattaca atccctttct ctattggaaa taattttatt ctccaacaat aacgccctac
    21361 aaacataagc atgaactttg cttgtaggac gcaatataat ttattttgct aatgacgttt
    21421 ctattgcctt aatctcatct ttagataaat taacaggttt ctctccatct ttgacatctt
    21481 cctgcaacgc tttttgagct tcttttgaat gatacaattc tacgatttta gcatatgttt
    21541 tgttatccaa gtctttgtca ttaactgcaa caatattaat atatggcttt actgcatctg
    21601 aatttgattt ttctaaaaat atcggatcat ttttaggatc tttacccgct ttagttgcta
    21661 caccgttatt aataactgca atatcgacat cagataaagc acgtgcagtt tgttgtgcat
    21721 ctactgcagt aatttttaaa tgttttggat ttgacgttat atctttcacc gtgcctgcta
    21781 atccgaaatc ttttttcagt tttattaaac cagctgcttc taatagttta agtgcacgtg
    21841 cttggtttga cacatcattt ggaatgacaa ctttagcacc atctttaacc tttttgacat
    21901 ctttaatttt atctgagtaa atgcccaacg gtgctaaaac tgttgtactt aatgctgaaa
    21961 tctttgttcc tttatgcgcc tttttatatt gatctaaaaa tgcaaaatgt tggaatgcat
    22021 tcatatcaat atcatcatca tttaatgctt tattcggtaa attgtaatct gagaagtgct
    22081 taatctccac atcaatatca tcttttttag ctaattcttt aaccttctcc caagccttag
    22141 tgtcatttga tgcgacacca attgttactt ttttatcatt gttaccacca cacgctgcta
    22201 ataatacaag tgctacgata actaacccaa tcaatctttt cattctatca attcctttca
    22261 aaatcttcac tatatatcat taatgtctac gtatgaatct agctagaaca ttccctagcg
    22321 tttgaatcac ttggacaata atgactaata caataacggt aataataatg accgtcgtat
    22381 caaatctttg ataaccatac actaaagcta agtctcctat accaccaccg ccaacagctc
    22441 ctgccatcgc cgtacttcca ataagtccaa taatcgcagt ggtaattgct aatactaacg
    22501 aacctaaagc ttcaggaatt aaaaaatatc taatgatttg tagtggtgaa gcgcccatcg
    22561 ctttcgccgc ttcaataatc ccctcgtcta cttccaataa tgagttttca acaagtcttg
    22621 caatgtaagg tgccacatat actgttaaag gcacgatggc agcagtcgta ccaattgaag
    22681 tacctactac taattttgtg aatggcacaa tcgcaattaa caaaataata aatggtagtg
    22741 accttaaaat attgattaaa ggatttaaaa cttgatgtat cactatattg ggccatatgc
    22801 cttgttttcg agtaattacc aataagacac ctaatggaat accaatcact gctcctaaaa
    22861 ataaagcaat agataccata tatagcgttt cgtacaatgc ttgtaataac tgtgcactgt
    22921 ctaaatcaga accaaacata tgttaatgca cctcctcaaa ttgaatattt ttctctttga
    22981 aatattgatt tattgccgtg tcttcaaatt gttgatccat attaaatcga agccacatat
    23041 aacatacggt gttaccttgt atttctgaca tagatgaaaa taaaatttta acctctctgc
    23101 cacaaatttg aatcaagtca tttataatcg gttgtgtcac ctgagtttcc tcgacgaaga
    23161 ttttataatc tttaaaatcg ccaacttgtt cgtcattcaa tcgacgaatc aatgatgtac
    23221 ttggctcagt ctgtataact gtagacacaa aattttgagc aatcgtcgtt ttaggatgac
    23281 taaacacctc tttaacagtt cctgtttcaa ccactttccc cttttccatt acagcaacac
    23341 gattacaaat gtctttaata acgcgcattt catgtgtaat catcataatt gtaatgccaa
    23401 aggtttgatt gacattcttt aataacgtca atatcgaagc agtcgttgct ggatccaatg
    23461 cgcttgttgc ttcatcgcat aggagtattt tcggattagt aacaagcgct cttgcaatag
    23521 ccaccctttg cttctgccca ccagataatt catcaggaaa ttggtctttt ttatcactca
    23581 atcctacaaa ttcaagcatt tccgttactc gttgcttaat ttctgttttg cttttcttac
    23641 ttaaaatgag tggcattgct acatttttaa atacggtagc tgaatttaat aaattgaaat
    23701 gctgaaatat cataccgata tctttcttaa tatccctcat cattttatcg ctataattcg
    23761 taatatcatg tccatctaca atcacttgtc cattcgaggc agcttcaaga tgattcacga
    23821 gtcttaccaa cgtacttttt cctgcaccac tatatccaat cacaccaaaa atatcattgc
    23881 gattgaccgt aaatgatacg tccttcaaag catctatttt ttgcttcttt ttattaaagg
    23941 tcttacttac ctgtcgaaac tcaatcatca tcgcccctcc tacaaattct tattaatcca
    24001 ctaggaataa gagcattata tgtaaaattg catatatcgt caatacaatt tgccgaattt
    24061 tctaaaaaat taaaaaataa gtaattcatg tgacaatgac gaattgtgag actactatga
    24121 catttatcaa attaaatcca taaaaatgtc caccaatcct ccacaacgca attactaaat
    24181 attaacatcg cacaaaaaag cactagcata ttcaagaaca acaaacgttg aactcaaaat
    24241 atatgccagt gctgctatta tttataaagt atctagtgct tgttttaaat catcgactaa
    24301 atcttcagta tcttcaatac ctacagaaat tcttacaagt ccgtctgtaa taccttcttt
    24361 agctcgaata tctgctggaa tggatgcatg tgtcatcaat gcaggtactg aaattaaact
    24421 ttccactgca cctaaacttt cagctaatgt gtaatacgat gttgctttaa tcaattgttt
    24481 ggcactttct gtatttttca cttcaaatgc aatcacacct gtatggccat ccgcttgagc
    24541 catatggaca tcatgattta aatgactttc aatacttgga tggaacactt gttgcacagc
    24601 tggatgtgct tgtaacattt taataatttc aataacgctg cgattaattt gttccatacg
    24661 taaacctaat gttttaatac ccctcacaag taaatagcta tcttgaggtc ctaaaatgcc
    24721 acctgttgaa tttgaaataa atgctaaacg ttctgcaagc ttgtcatccg atgttgcaac
    24781 taaaccagca acgacatcac tatgtccacc taaatatttc gttgcagaat gtaagacaat
    24841 atcgatacct aaatctaatg gattctgata ataaggtgtc ataaatgtgt tatcaacaac
    24901 tgaaatcaaa ccgtgttctt tcgcaatttc agcagacttt ttaatgtcag taacacgtaa
    24961 taatggatta gaaggtgttt caataaacaa catctttgtt gttgggcgta tcgcttgtac
    25021 aattgaatct gtatgcgttg tatctacaaa atccacttca atgccaaatc gtgtaaatac
    25081 ttttgtcaat gcgcgataag taccgccgta tacatctgaa tttaaaataa tatgatctcc
    25141 tttgtccaac agcataacaa ctgcactgat tgctgcaaca cctgaactaa atgcaaagcc
    25201 atgtttgcca ttttctaatg tcgcaataac gctttctaca gaacttcttg ttggattcgc
    25261 agtacgagaa tattcatatc cttgacgtaa atcaccaata tcatcttgta aatatgtact
    25321 tgtttgataa attggtgttg taacggcacc tgtataatcg tctgttgtgt gcccaccatg
    25381 aattaattta gttttcttgt tcattattat tctcctcata attaaatatt tgcttagaca
    25441 tatatcgatc actaccatct ggaaatacga caacaatcgt accttcagat aattgcgctt
    25501 ttaaattcaa tgcaccttgt aatgctgcac ctgaagaact gcctactaac aacccttcat
    25561 ttatagccaa acttttgaca tttcgaaagg catcttgatc tttaatcgta aatatcccat
    25621 ctacaagacg tctctctaaa aatatcggcc atttctcaga accgatacct tcagtgtcat
    25681 gtgcatgagc tggccctcca tttaacacgg acccttctgg ctcaacggca taacattgca
    25741 cgtgatgttg ctttaaataa cgtgcggtac ctgtaaatgt accgccagag ccaataccag
    25801 ccacaaaata atcaatttgc tgtaatgctg aagtcaattc gggtcccaat gtatgaaaat
    25861 atgtatccgg attatgttcg gattcaaatt gattcatata aacggcacca tatttttcag
    25921 cataggaacg tgcagctaat tgtgccccat gcataccttc agactgactc gtccttgaaa
    25981 cttctgcacc aagcgctatc ataatattaa tcttttcttc tgaaaaacca tacggcgcaa
    26041 agatcttaca tttcaaatga tgtctattcg ctgcaatagc taaccctatg cctgtattac
    26101 cagcagtcgc ttcaacaata gtttgacctg cacgcacacg cccttcttga attgccttct
    26161 ctactaaata tttcccgagt ctgtctttaa cactgcctcc aggattccat tgttcaagct
    26221 tggcataaat tttaacttta tcatcactat aatgttctaa cagtactaat ggtgtattgc
    26281 caattaaatc ataagtaatc atagatgcac cctcatctga catgccgatc aaatgaatga
    26341 aacctttctt catgtctcaa ttttaattct tacttttcag ataagaatta taaacgacat
    26401 tttgttattt tgcaattatc taagtttcga ttaattcaga accagtacta aattttcaat
    26461 tccaaacaaa aaaacacctg agcaacacaa atacttgtgt gtcagatgct tctatatatt
    26521 aactaaataa ttgcacgata aagactaaaa taataacgac aggcatcgca tacttaatta
    26581 agtaatacca accactgaat aatctaaatc gatctttacc aaaatattgt tgtaataatt
    26641 ttttatctaa taattgtcct acgacaagcg tagtacctaa tgcgcctaat ggcatcaata
    26701 cattcgaaac gatgaaatcc atattatcaa aaatcgttcc cgcaccgaat cttacatctt
    26761 ttaagatacc aaaagataag gttgctggaa tactaatgat aaatactaaa atactaccga
    26821 tcactgcgac ttttttacgt tttgtattgt cattcttcgt gaagttagaa acatttaatt
    26881 ctaataaaga aatagatgac gttaaagccg caaataagaa cagcactaag aatcccaaat
    26941 agaataatgt gcctagatgc atttgactaa agaccattgg cagtacttta aataataatc
    27001 caggcccttc ttgtggttca tagccaaaac tatgtaaagc cggaaatata gctagacctg
    27061 ccaatacaga tacaaagata ttcataacaa cgatagaaat agctgatgac ttaatcgtca
    27121 tgtctttaga ggcataactc gcataagtaa tcatacctgt agttcctaat gataacgtaa
    27181 agaatgattg acctagcgca aacaagatgc catcagcagt aatctctgat actcttggtt
    27241 gtaaaataaa tttcacacct tctaaggcgc catctaatgt taaagactta atcacaatga
    27301 cgattaaaaa gacaaacagc aatggcatca taactttcga tgccttttct aatccttttt
    27361 caacacctaa catgacaata atcatcgtag cgaatatgaa tataccttgc cctagaacgg
    27421 ttaaccaagg atttgatatt accgcttcaa aattcatttc ttggagatga ttgatgcgtt
    27481 gaaatataac taattgccat aatacttgtc cgatgtaaat gacaatccaa ccaccgataa
    27541 cactatagaa gccaaataaa ataaacactg ccaaattacc gttccagcca atgatattga
    27601 gccatttttt accagttaat ttactatata tttgtgttgt atatgtccgt cccattttcc
    27661 caacagtgaa ttccataatg agtaatggca acccaacaaa aatggtgaat attaagaaca
    27721 tagctagaaa ggcaccgccg ccataaatcc ctgccatata tgggaatttc cacatggcac
    27781 caagaccgat tgcagaaccc gcactagcta aaataaatcc agttgatgac ttccattgtg
    27841 attgttgtct tttcatcatt cacatctcct ttaaagcgtt aaagcgtcta cgtgtttaag
    27901 taacactcta acatgtatgt aaataacatg caatacacct gcatcaaaat tgtttcatta
    27961 atacggtagc acctggattt atcataatat aatcaactct tgtaatgtgt tgtcattacg
    28021 ctacattaaa ttttctggtt caacgctgta tacgtgcgcc aatatctcgc cacatacaac
    28081 accatctata tccccaacat ctcgccacat aacaacttca ggtgcaatgc ccataaaatt
    28141 tccacgacat tacaatacaa atcaacttct ggtataactc cataatattt ccacgacatc
    28201 acactacaaa ttaactttag ctaagttccc tatactatca cgccacaaaa aacactcaaa
    28261 tgacaaatat aataacatcg tcactcaagt gttaattttt aaaatcttca attatgttaa
    28321 agcattgaaa aagcgaataa cattttcttc taaaatatct ctgccttctc catgcgtcat
    28381 taaatgatgc gaatccttca gaccatttaa ttctttgtct actgatccta aatgttcata
    28441 aataaaatgt gcacttgttt cataggatgg cgcatcttta ccaccaaata atatattagc
    28501 tggcatttta ataacattta aatttgtcat gatatcatca ataaaatgtt gaaacttcgt
    28561 aatttcacct tcataatcat caattgctgc taattgcgca ctcgatgctt gctgatctaa
    28621 atttaaaata ttcgacatgc gttgactata ataaactaaa tgttctatta agccatcgtc
    28681 actcttttcc tttggtgctg acatgacagc gatacgtttc aaaggatagt gttgcgccaa
    28741 ttttaatgtc attaatccac ctaaagacac acccgttgca ctgatagatt cataaccttc
    28801 attgactaaa aattggtaag ctttctcaac ttcttcccac caatcatcta cattatatgt
    28861 catgaaatct ttcaacaata aaccatgacc tggataattc ggtgcataac aactaaatcc
    28921 ttggtcattt aactcagctg caagatgctt cacatcccga tttgtacctg taaatgaatg
    28981 taataataat atcgcatgtc catttgtgcc ttttaaatac gatggactcg gtgttttaat
    29041 tctcattttt ctatatacct ccactatgtc taaagatgtt tgctaaacgc gttgtcgtcg
    29101 atgattaata aagtatgggt ataccattaa gaataacgct acccaaatga ttgctagtga
    29161 cgtgccgcca atgacatctg aaaagtaatg tgcatgaaaa taaaggcgac aaaataatat
    29221 gctaagccat aatattccca taaccaacgc actcaacact tttgttattg tcttagcagc
    29281 aagtgaaata ataatgatca ttaaggcgaa atataataat gtgctggcgt tggaatgtcc
    29341 actcggaaat gaaaagcctg tatcaacggc taaatgatta tatggtcttg gacgtaatac
    29401 agtatcttta attaatttgt tcatgatgac acctgaaacc aaatatgtca caaaccaaac
    29461 cgctaaatgc ctctgtttaa taaacagtat gattgtgacg ataatggaaa tcaacacgac
    29521 acctttgaca tctccaattt ccgcactaaa cgtcatatag taattaaaca aattgttaac
    29581 atactgacgt tgtggctcac cgaaataatc tgtaaaccat gttaatgatc ccatatctat
    29641 atttttaagc cattcttgat ttgtcactac actgtaaaac atacctataa atacaatcag
    29701 cgcgattaaa aataaaggca ctgtcatttt cggtgatgtt aattttttat ctatcatctt
    29761 acaatctcct cgtatcatca ttttcatttt acaaatgtta tccataatat caatgtgcca
    29821 caaatttcac tttaccgaca atatcaaaat tataaagttc atattgttat gtatattgca
    29881 aataaaacat tgtataattg aaataacaat attttgctat tttcaattta gtacgattta
    29941 tatttattat acagaggggg taaggcgtat caatagagtt atttttgtct atatagcgtt
    30001 aatcattaca ttagttagat tctttacccc tattcatcca tcattttcaa atttgattta
    30061 ctggatattt gtattatatt ttattcctat tatactatgc gttatcggtt tcaaggccga
    30121 aaaacttatt gcaacaatgg tcattatacc taatttttta ggaatacttt atcgattata
    30181 tgcctacgtc acacatatac tctttatgta aaaggatagt gggcatgtct cgcaacaaat
    30241 atgcttgcgt cgacttgtca ccattcgcaa attttatgac atacgccttt cacgggctat
    30301 attcagaccc acgcattcat ccacgtaata aacacatcat gtaatagaaa aacagcacac
    30361 ccaaatatat ggcgttgcgc tgtttaacca agcatacttc tatagcttta ataagccagc
    30421 agaagcatac ctaaccttct taaatatgct tttccaaatt atcctcaagt ttgaatacga
    30481 taatacgttc acctgtaact gtacttaaat cactatggaa gctcatcact ttgatacctg
    30541 taattttaaa aatgatatca ttcaaatctt gctcaccgga ttcaactaat tcagaacgtg
    30601 ttcgtttaat atttaataat ccttcattcg tactacatac acgatattca gctggcgtta
    30661 agataccttg taaactaata atcaccatat ctcttaaaat gtctgattta actgacaagg
    30721 aacctctgcc aaggaaatct ttttcccatt gcgtaatggc tttactgatt tcagcttcga
    30781 tttcaccttt cgttcttttc atatcactaa ctccaataat attaaaattg attacttcat
    30841 ctttgtatcg ttatcacgac atcaactatt acattaagtt tatcattttt agtatatttt
    30901 aagaagctag aacattgtag atatgatgat atattagtta cttagcatcg caacatatca
    30961 tcgttaaatc caacttttaa aacgcccttc ctcattaacg ctcattaaac gcagccaatg
    31021 attagacacc ttcctagcga aatgctcatt attcgcgagt agtcttgcta caacatagtc
    31081 gggtgcctga ataacgacaa gtaaacgaat tggcgaatga tacatcgtcc gatcagcagc
    31141 cataacagat tgccatgata agccatacat cagatcactc gcattacctt gcatgacacc
    31201 aacacctgac gtgacggttt gtgtcgcttt attcccactt ccgtaaaaat gcggcgcaac
    31261 tgtcgacgca taatattgta aattaatcca ttgtgccaca agtgccggac cagaaatgat
    31321 ggtatttaat aatgtgccat ctttatcttt acgccaatca taattgtgta aaaatgtccg
    31381 cccttctaaa tcaatgcctt ttgttaattg gcgtcgtcca attataaatg atgcattttt
    31441 agccaatccc cattctggac gtacctcact ccaatcactc gcaaaccgct gcgcttcttc
    31501 cactggatga ttcacacgac caatcgttgg cagtttgtcc aaacgttcgc gattcgcgtg
    31561 ttcagaaatc atcggcatcg cgtcattcaa tgattcatat gcatctaaag caatagaaga
    31621 taatgtgtct ggcacatata cccatgccaa cgtatcagta gacgtatgat gttctgctac
    31681 cgcaaaaaca gttgtctctg gaatatacac acctgattgt tttaatcctt gtctgacatt
    31741 tggacgatta catatcatcg ctaataactt agcattaaaa ccgcttgatg cgccaccaca
    31801 agccccacat tcaagtgatg catgatgtgg attattgtga gaatgactag catgacctgc
    31861 taacacaacg aacggcgcaa atgcttcggt taaatccatc aatttcaacg cttgtaacgc
    31921 gaaatcaatt tgctcttgct cagtaaatcc aacaggtaag tctgatgttc ggtcaaactc
    31981 acgatcaatc gtcaactttg tttcaggctt tttcaaccac ttttgtttta ttttttgtaa
    32041 agacgcgcga ctttttctag gcataatcga attgacaatg gtacttaagc ttaaaaatgg
    32101 cccacttaat tcaggcaata acagactagg catgacatta tttttcatca atttaaatgt
    32161 gtaaaacatc gatgacattg tctgttgctg ttgtcgataa acattcatat cgtagcggtc
    32221 tgcaaattct ttaatgcgat atgccggcgg taccatgaca ggtaatgaat catgtttgaa
    32281 ttgttcgtct acggcatctt tttgaatagg taatccaaag aagcctgcaa taccaatcgt
    32341 ttcaaagggc cctgctgctt cgatatgtct acgaaatggt tctgaacgaa catctataca
    32401 aaatgcaatt tgcgctttcg ttgatgtgcc catctgattt agctcgctat tattttcatc
    32461 aactgcttgt gtgtcattta acaatactga atgtggctga ttagcgttat cattttctga
    32521 gacatttact tggtttacat ctaatgcgcc cgccacactt tcatgacctg ctttaatttt
    32581 ttgttttaac tgagattcgt atgtcatttc ccaggcaatt agccataaat ttttaaatac
    32641 atttttattc atagttgctg caaaatgaat aaacgtttga atttcattga cgtcatgttg
    32701 tagtaataca tcgctaggca tatcactgta gtaacaccat gatgcaacag tttgcttaaa
    32761 ccaattttcc gatctacttt cacaatcttt agcgactgac ttaaactcat caccaactag
    32821 caattgttcg acaactaacc gaattgccaa ataatccgtt aacaaatgtt gttcaaagta
    32881 atgctgttgt gaacggtaat acaacatacc tgcccaaccc ggtaacgcca aaagatgtcc
    32941 ttcaacataa gcttggtagt cttcctgatc tattgaaaaa tgagttaata ctgactctat
    33001 cgtcatttca ggatcattgg gtaagccttt aatcacttgg cgctgtgctt tagtaaaact
    33061 atggtcatgt tgcgctaaat gcaaccatgc atggtaaaaa ctttgctcac gcttcggcat
    33121 tgtccaactc gatagaaatt gatcgatata aagtttcgtc catttaatca tttgacgatt
    33181 cacttgttcg ctaagtggct caccttgttc atctattatt gcatcactca tcggacgtac
    33241 atcatagtga tgatatgatt cagccatatc acgttttgat ttttctaata gtagatcagc
    33301 aacaacatca acatttgaat gattcatata tgatgcaggt acgtctttta atgttttaat
    33361 gttatcaata taaagattga tgtagtgttg cgggatattg tagtgatgtt caagtaacat
    33421 atcagtaaca agttgattaa agacactttc atctaattca ccacgtgcca cagcgctttc
    33481 tattaatgct ttatttggga aaatatccac atctcgaaca tcacgtaacc attttgcgac
    33541 atcttcaaac gtatccgctt ctaatccttc ccatggattt cgtgctgcaa aaatcgaaat
    33601 tggtgataat ggtgtaataa cacgtttcgc attttcaatg actgaattga tatttaactg
    33661 tgttgtcata cctttcacct cctataaata cttcttcaaa taattcggat gactttctat
    33721 cgctttcgag cgtgcttcac ctagattaac taaccacacg tacaataccg caaaagcctt
    33781 agagtatcga tgccgcgcca cccaaatact taataaactg ccaaagatta aaataacaac
    33841 actaatgatg acactcactg taggcggcgt tgtcgcatgt gttgttatat tttgtaatac
    33901 agcgtaaaaa taattatgtg tgatgacgta gataaatgtc acgattgcaa tcaaaatcat
    33961 accaacaaga cgtgccatgc gtcctttact aaaggctacc atttgattcc aagatacaag
    34021 taatgaccat cctagaatga gtgcacttaa cacttcatat gcacttctgt cactactcat
    34081 ccaaaataga aatgccacga taatagctaa tacacgtccc atgacaatcc agccataagc
    34141 gtctttagca gatgcttgtt ttggaatatt gaatcgcttc acgatagaac ctgattgtaa
    34201 aaataatgtt gctttaaaaa taccgtgcaa tattaaatga ataatcgctg ctgaatatac
    34261 acccaatgca cattgaacta acataaagcc catttgactc atcgtagagc ccactaattg
    34321 gcgtttatag tcaacttgaa ctaagctaat acccgatcct aacaatacag aaatactaga
    34381 aaggataagt aataatgata acgcaaatcc attatcaaat atcggcgcaa aacgagttag
    34441 aataacacca cctgcattca caattcctgc atgcataatt gccgatactg gcgttggtgc
    34501 cgttacagat tcaatcaacc atcgatgaaa aggaaattgt gctgccggta tcatgacagc
    34561 taatacaagt agtacattcg tcaacaatga ccatgtcgga tgaactatat gttgtggtac
    34621 ccgccactcg ccagtcgcaa tataaatagt tacaattgct ccaacgaatg caagccaacc
    34681 acataaaaat gtcatgcttg ataatttcgc agactcacgt ggcactttcc aaaaacgatt
    34741 aacgttcatc agcaatgtta aacataataa tgtaataccc cagcagagtg ccatcagtct
    34801 taagtcttca gacatccatg ctaaagatgc aaacgacgta atcgcagtga acaatggaaa
    34861 gtaatgtcta taatgatgat cacctagtaa atatcgcatt gaaaactttt gaataataaa
    34921 gccaagcgcc attacaaagc cagctaataa ccaagataaa cgatctattt taaatggacc
    34981 taagacatgt tgaccatgaa taccgaaaaa gccaatgact gcaaataata ctggcatgac
    35041 tagtatgtat aaatgtaatt taatatatct cattggcata actggtgcta aaaacaacaa
    35101 gccacttatc aatgcaatga taagcgcaat aacaaacagt gaaaatagca attgaaaact
    35161 taacactgca taacctcctt atttctaatc tctcgcataa ttgcttatgt ataaaaataa
    35221 aaacctacaa tagtagattc tgtacataat ggcagaaaat ttactattgc aggtttcagt
    35281 ttaactagac actgcatcac ggtacgttga tataccttgt tgcagtgttc tctttaagcg
    35341 tgctcccatg cacatatgta tataaaatgt tacttctgtc tgttcaattc atcttcataa
    35401 atatgctttg cctagacgag acctaacgtg ttattcgttt taaacttata acataaaata
    35461 taattaaatt tctgcttcat gtcaaattca tgagcttaac ctctattaaa ccaatgattg
    35521 taaagatttt gtaaatgcac ctgtacagtt aggcagtatt tcccgtcctt ttaaaataaa
    35581 aaattcgcag ttatgatcat aacaattcaa gttaggaaaa aaatcaatta cgcacaagat
    35641 aactatgtac aatgaagtta actcataagc aaaggaggta atcttaatgg gtatcatcgc
    35701 tggcatcatt aaagttatca aaagcttaat cgaacaattc actggtaaat aagatttcat
    35761 aacaaacaaa ggaggtcttt cacatgggta tcattgcagg aatcattaaa ttcattaaag
    35821 gattaattga gaaattcact ggtaagtaag ttataaaaat ctcatagata tgaacatctt
    35881 atttgaaggg ggccattcac atggaattcg tagcaaaatt attcaaattc tttaaagatt
    35941 tacttggtaa atttttaggt aacaactaat ctcaaacatt aacgatcaac aactcatcac
    36001 tatgttaaat caacatacag gaggacaaaa cgatggctat tgtaggtact atcattaaaa
    36061 tcatcaaagc aattatcgac attttcgcaa aataatttaa gcgaattgaa tacttaaaat
    36121 tctcaggcca ctataccaat agggatggtg gctttgcttt gtgttgattt attgatgtga
    36181 ggtgagtctt gttagtttgt tgcaaataaa tggtcttggt gttttttgta taggacgttc
    36241 ttagtgggac atacggaata ttcgtgatct ttgtagtctg acgcgttata tttttgtggc
    36301 gtgttttatg tttgatactc gagttctgag acattcatga tttggcatgc gaaatcttaa
    36361 tgattttcat gatctagcgc aagatatatt ggccacgtgc ggaattgcgt tgcacgttta
    36421 gactgaaaca ctcgtgtgac cgtaagtgtt aatagtacat tgatagctgc atttacttca
    36481 ctcattttta tgactgttaa acaatgattg taccttcaat taacagttgg tacgatggtt
    36541 ttgccatttt tcatcaacgt aaatataaaa aggactaaga cacatacatg tcctagccct
    36601 atggataaaa tgcaaatttc tgctttatca aaactatcac actttagata gattgaaaac
    36661 aaaaagatcc taagaacacc ttaacttttt attaattgtc ataaattgca aacaattaag
    36721 ccacaattca aaaatgatta tacttcattc aacttatcgt gctggtctaa tttgccattg
    36781 atatggatct tcaaattgtt gccaatctgc atcaatttct tgcgcattga ctaagcatgc
    36841 gtcgagttct tttgttaatt tttcttcatc taattctgta ccaataatga caaattgtgt
    36901 atgacgatcg ccatattctg gatcccattc agctgcgaca tcttgacgtt ctgctaatat
    36961 ttgtgtttgt tgcgcttcag acatactagc cacccaatat gtaactggat gaatattgca
    37021 agatgaccct gcttgagata ataaacatgc tacgtgattg tattgtgcta gccatacgat
    37081 accttttgat cgaacgacat tatttggcat gctttctaac caatcattga accttttagc
    37141 atggaaaggt agacgacgtt tatatacaaa cgatgatata ccatattctt ctgtttcagg
    37201 tgtatgcgat gcatgcccac cagactcaag ttctttgatc catcctgctg actcgctcgc
    37261 tttttcaaaa tcaaaacgct gcgtattcaa gacttctttt aaatctactt cagaatttgt
    37321 tgtcttaata attttagcag tcggttgcaa tgcgcttaac attttttcta acttcgctag
    37381 ttcttcttca ctaattaaat caattttatt aataatcaat acatcacaaa attcaacttg
    37441 gtcaattaat aaatcagcaa tcgaacgctc atctgtttcg tcaacgcttt gatcacgatc
    37501 catcaataaa tcttctgagt tgatgtcatg tacgaagcgg ttagcatcca caactgtaac
    37561 cattgtatct aaacggcaaa tcgctgtaag atcaatgcca agttcatcat caatatatga
    37621 gaaagtttgt gcaacaggta ctggctctga aatccctgtt gactcaataa caatttgatc
    37681 gatgccacct tttttcacta aacgctcaac ttcttttaat aaatcgtctc taagtgtaca
    37741 acagatacaa ccattagaaa gttcgactaa tttttcatct gtacgcgata gtcccccacc
    37801 atctgcgaca agatctttat cgatatttac ttcactcata tcatttacaa ttaccgcgat
    37861 acgtcgacct tctcgatttt gtaaaatatg atttaacaac gttgtcttcc ccgagcctaa
    37921 ataaccactt aataccgtaa ctggaatttt agccatatat ataccctcac ttcaatttat
    37981 ttgtaaatag gaataattcc gttttacatt atataggagc gtttcctctt tcgcaatctt
    38041 cgataataaa aaaatagtat acttaattaa attattgagc gctttacttt ataatggaga
    38101 caaagatata tctcacgaaa gagaatcgag gtgtataaac atgttatttg tcattttagt
    38161 tttatatgtt actggtattg catttattct actcagtgtt tttggttcaa agactgaagg
    38221 attatctacg aaacatactt tatataccat tggcagtgct attataacga ttgctatttt
    38281 catttcaatt ggctatgcca ttcaatactt aactgcagcg ctttatggtt tgtaaggtga
    38341 aggtgatgag taacgggtag ttcgggagag gttaacttgc gttgattttg ataaagtgat
    38401 catagctttt agtacttgag gatttttatt gttgctgtta cgaatgtggt catgtttaat
    38461 gcgggacagt aatttaagtt gtttttttac aattgagagt gtgatatttc gattcggttc
    38521 gaattacttt acatgggaat aatataaatt aaaaagaagc ggcctagtgt cagttgtgaa
    38581 tatactgaac attggtcgct ttatttagta gtatgatatg tagtttagct attaattttt
    38641 ttcaggtcat ccttaatgct gtctatctca gacatggcac ttttaaccca atctccttga
    38701 gctgcacctt taaaattagc tttaaaagct tcgcaatgtt gcgccatttg ttcaattaat
    38761 actttttctt caccttttaa tccgttttca atatctttgt atttatgctt atgttcaggt
    38821 gcaataactg tgcgaatatt ttctttttgc gcttccattt tagatatgag attaagtgtt
    38881 tctactgtag tacttatatc tggcattctt aaggtcatat ctggttctat tagagtcatt
    38941 taatctcctc caaattatca gtcacttagc ttatctaact gcttttcata agactttttt
    39001 aagtcttctt tatattcttc taatttccat tcttgctttc taattatctc attgatttct
    39061 tgctcatacg cttctatatt tctcgtcatt ttcctttggg catcttcact ataattcatt
    39121 tcgtgcttca aatattgaaa aacatcataa ctacgctcat tttcttttcg agttctatgc
    39181 ctaagttcat ttaaattttg cagttcattc tctatacgtt ttttctcaga ttcatatgat
    39241 tcttctaatt catttttatt attaatttga ttaaacatca tagtaatcca cctatttgtg
    39301 atgcaagcac ttgatcttta tcaacgattt cattaatact gagtctaact ctcataatat
    39361 aatttgatac agaagaccct atcgttgaaa gttgtctttc ttttgcagat atcatttgtt
    39421 caggttctct taccatacta ctttcattca cttgaccttc atctaaagca ttgagaactc
    39481 catttacact caaatcttca cctacagctt ttccagcttt ttgagcgtct tcccaatttt
    39541 ttccaaattt ctttttcttt tcattataca tttttttgat ttgattaatt tcatcgtcta
    39601 tcagttgact gaatgactgc gcaattgtta aagctgttaa actttctaaa actttttgct
    39661 gcgaagaaga taatcctcct ccatttgtag tcatcatatt tgctctaagt aattctatac
    39721 tagctaattt agagatagca ttatttgaat tctttacaaa tgacttatta ttttcttctg
    39781 cataaccttg tagtttttta agttcttttt ttatagcttt ttgttcttct tcggtcagaa
    39841 agccttccat ttcatgacct ttaccatttt taaagacttt atcattaccg aaaaaaacat
    39901 aatgcttata tttcttcaga ttttctgtca atgcatcatt ttctgcaaca aacctagtaa
    39961 tattaccttt ataattttta tataattcta ataaacgttt tccatctggg gaattaggat
    40021 tcaaactagt tatagaaata ggagctggat tataaaccac aatgttcgga ataccattac
    40081 tcattcctag aactaccgca tctcttccac ctagtgaatg tccagtaata aaatcaatgt
    40141 catacttact ttttatttta tttataaatt cttgtgtatt cgcatatcta tagttttgat
    40201 cagatgcagg agaatataat attttaagat ctgcatatcc atctttaact gtttccaaag
    40261 cattggtaac attttgtctt gaaaattcac cttcttttaa ctttttaaag gcttcgtctt
    40321 gtaaattagt cccagtcatc ccgagagtta ctttgcctgt atctttattt aaaaaagcag
    40381 aagttgttac tccagtaaga gaatcagtgt aactatcaac atattcaaga ttagaaggga
    40441 attttttcat ttttttcaaa tcattaaatt gattaatcac gtctcttttt gatagaccat
    40501 tattattagc attctcaact tcgtatgacg ctttcgaaat agcacttgta gactcagtat
    40561 tttgattaat tgcttttaaa tctattttat acttcctact caacattctc accttcagac
    40621 ggtccaaaat tcaaataatc tcttaatgtt gtctcttgac ctttatcttt tgaaaattta
    40681 tattctattt ttttatatcc tactgagctt ccttttatat caccatctat atataatttt
    40741 agatttggag agttattagt tgtaattgga taaatttctc taagtttttt tacattttta
    40801 tcactattgt tcattttata ttgtgcttca tagctcggta cgttttcatt agttgttata
    40861 tttccatctt gataattttt taaatcttta aaatcagcat actgactaaa aaatttaaaa
    40921 ttttcaatt
//
LOCUS       DALAGF010000013        40666 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.013, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000013 DALAGF010000000
VERSION     DALAGF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 40666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40666
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.013"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            60..1178
                     /gene="ald"
                     /locus_tag="O2692_000644"
     CDS             60..1178
                     /gene="ald"
                     /locus_tag="O2692_000644"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCZ4298285.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            1273..2313
                     /gene="tdcB"
                     /locus_tag="O2692_000645"
     CDS             1273..2313
                     /gene="tdcB"
                     /locus_tag="O2692_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCZ4298286.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            2344..3666
                     /locus_tag="O2692_000646"
     CDS             2344..3666
                     /locus_tag="O2692_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ4298287.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            3823..5214
                     /gene="norB"
                     /locus_tag="O2692_000647"
     CDS             3823..5214
                     /gene="norB"
                     /locus_tag="O2692_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HCZ4298288.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
     gene            5612..36550
                     /gene="ebh"
                     /locus_tag="O2692_000648"
     CDS             5612..36550
                     /gene="ebh"
                     /locus_tag="O2692_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HCZ4298289.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGFNTSQAHAAETNQ
                     PASVVKQKQQSNNEQTENRESQVQNSQNSQNGQSLSATHENEQPNISQANLVDQKVAQ
                     SSTTNDEQPASQNVNTKKDSATAATTQPDKEQSKHKQNESQSANKNGNDNRAAHVENH
                     EANVVTASDSSDNGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKIN
                     TLLGSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSNRIQTRSVESRAAEPRS
                     VSDYQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDSKEIALM
                     TAKQTGDGYQWVIKFNKGHAPHQNMIFWFALPADQVPVGRTDFVTVNSDGTNVQWSHG
                     AGAGANKPLQQMWEYGVNDPHRSHDFKIRNRSGQVIYDWPTVHIYSLEDLSRASDYFS
                     EAGATPATKAFGRQNFEYINGQKPAESPGVPKVYTFIGQGDASYTISFKTQGPTVNKL
                     YYAAGGRALEYNQLFMYSQLYVESTQDHQQRLNGLRQVVNRTYRIGTTKRVEVSQGNV
                     QTKKVLESTNLNIDDFVDDPLSYVKTPSNKVLGFYSNNANTNAFRPGGAQQLNEYQLS
                     QLFTDQKLQEAARTRNPIRLMIGFDYPDAYGNSETLVPVNLTVLPEIQHNIKFFKNDD
                     TQNIAEKPFSKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQ
                     ITGIPRTLHIENSTNRPNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTP
                     TITTTAEQLRGTALQKVPVNISGIPLDPSALVYLVAPTNQTTNGGSEADQIPSGYTIL
                     ATGTPDGVHNTITIRPQDYVVFIPPVGKQIRAVVYYNKVVASNMSNAVTILPDDIPPT
                     INNPVGINAKYYRGDEVNFTMGVSDRHSGIKNTTITTLPNGWTSNLTKADKNNGSLSI
                     TGRVSMNQAFNSDITFKVSATDNVNNTTNDSQSKHVSIHVGKISEDAHPIVLGNTEKV
                     VVVNPTAVSNDEKQSIITAFMNKNQNIRGYLASTDPVTVDNNGNVTLHYRDGSSTTLD
                     ATNVMTYEPVVKPEYQTVNAAKTATVTIAKGQSFSIGDIKQYFTLSNGQPIPSGTFTN
                     ITSDRTIPTAQEVSQMNAGTQLYHITATNAYHKDSEDFYISLKIIDVKQPEGDQRVYR
                     TSTYDLTTDEISKVKQAFINANRDVITLAEGDISVTNTPNGANVSTITVNINKGRLTK
                     SFASNLANMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGT
                     QITTNDILTMLKATTTVPGLRNNITGNEKSQAEAGGRPNFRTTGYSQSNATTDGQRQF
                     TLNGQVIQVLDIINPSNGYGGQPVTNSNTRANHSNSTVVNVNEPAANGAGAFTIDHVV
                     KSNSTHNASDAVYKAQLYLTPYGPKQYVEHLNQNTGNTTDAINIYFVPSDLVNPTISV
                     GNYTNHQVFSGETFTNTITANDNFGVQSVTVPNTSQITGTVDNNHQHVSATAPNVTSA
                     TNKTINLLATDTSGNTATTSFNVTVKPLRDKYRVGTSSTAANPVRIANISNNATVSQA
                     DQTTIINSLTFTETVPNRSYARASANEITSKTVSNVSRTGNNANVTVTVTYQDGTTST
                     VTVPVKHVIPEIVAHSHYTVQGQDFPAGNGSSASDYFKLSNGSDIADATITWVSGQAP
                     NKDNTRIGEDITVTAHILIDGETTPITKTATYKVVRTVPKHVFETARGVLYPGVSDMY
                     DAKQYVKPVNNSWSTNAQHMNFQFVGTYGPNKDVVGISTRLIRVTYDNRQTEDLTILS
                     KVKPDPPRIDANSVTYKAGLTNQEIKVNNVLNNSSVKLFKADNTPLNVTNITHGSGFS
                     SVVTVSDALPNGGIKAKSSISMNNVTYTTQDEHGQVVTVTRNESVDSNDSATVTVTPQ
                     LQATTEGAVFIKGGDGFDFGHVERFIQNPPHGATVAWHDSPDTWKNTVGNTHKTAVVT
                     LPNGQGTRNVEVPVKVYPVANAKAPSRDVKGQNLTNGTDAMNYITFDPNTNTNGITAA
                     WANRQQPNNQQAGVQHLNVDVTYPGISAAKRVPVTVNVYQFEFPQTTYTTTVGGTLAS
                     GTQASGYAHMQNATGLPTDGFTYKWNRDTTGTNDANWSAMNKPNVAKVVNAKYDVIYN
                     GHTFATSLPAKFVVKDVQPAKPTVTETAAGAITIAPGANQTVNTHAGNVTTYADKLVI
                     KRNGNVVTTFTRRNNTSPWVKEASAATVAGIAGTNNGITVAAGTFNPADTIQVVATQG
                     SGETVSDEQRSDDFTVVAPQPNQATTKIWQNGHIDITPNNPSGHLINPTQAMDIAYTE
                     KVGNGAEHSKTINVVRGQNNQWTIANKPDYVTLDAQTGKVTFNANTIKPNSSITITPK
                     AGTGHSVSSNPSTLTAPAAHTVNTTEIVKDYGSNVTAAEINNAVQVANKRTATIKNGT
                     AMPTNLAGGSTTTIPVTVTYNDGSTEEVQESIFTKADKRELITAKNHLDDPVSTEGKK
                     PGTITQYNNAMHNAQQQINTAKTEAQQVINNERATPQQVSDALTKVRAAQTKIDQAKA
                     LLQNKEDNSQLVTSKNNLQSSVNQVPSTAGMTQQSIDNYNAKKREAETEITAAQRVID
                     NGDATAQQISDEKHRVDNALTALNQAKHDLTADTHALEQAVQQLNRTGTTTGKKPASI
                     TAYNNSIRALQSDLTSAKNSANAIIQKPIRTVQEVQSALTNVNRVNERLTQAINQLVP
                     LADNSALKTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTESTNAQNVINNGDA
                     TDQQIAAEKTKVEEKYNSLKQAIAGLTPDLAPLQTAKTQLQNDIDQPTSTTGMTSASI
                     AAFNEKLSAARTKIQEIDRVLASHPDVATIRQNVTAANAAKSALDQARNGLTVDKAPL
                     ENAKNQLQHSIDTQTSTTGMTQDSINAYNAKLTAARNKIQQINQVLAGSPTVEQINTN
                     TSTANQAKSDLDHARQALTPDKAPLQTAKTQLEQSINQPTDTTGMTTASLNAYNQKLQ
                     AARQKLTEINQVLNGNPTVQNINDKVTEANQAKDQLNTARQGLTLDRQPALTTLHGAS
                     NLNQAQQNNFTQQINAAQNHAALETIKSNITALNTAMTKLKDSVADNNTIKSDQNYTD
                     ATPANKQAYDNAVNAAKGVIGETTNPTMDVNTVNQKAASVKSTKDALDGQQNLQRAKT
                     EATNAITHASDLNQAQKNALTQQVNSAQNVQAVNDIKQTTQSLNTAMTGLKRGVANHN
                     QVVQSDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTSKEHAL
                     NGEAKLNAAKQEANTALGHLNNLNNAQRQNLQSQINGAHQIDAVNTIKQNATNLNSAM
                     GNLRQAVADKDQVKRTEDYADADTAKQNAYNSAVSSAETIINQTTNPTMSVDDVNRAT
                     SAVTSNKNALNGYEKLAQSKTDAARAIDALPHLNNAQKADVKSKINAASNIAGVNTVK
                     QQGTDLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQN
                     KTKDQVTEAMNQVNSAKNNLDGTRLLDQAKQTAKQQLNNMTHLTTAQKTNLTNQINSG
                     TTVAGVQTVQSNANTLDQAMNTLRQSIANKDATKASEDYVDANNDKQTAYNNAVAAAE
                     TIINANSNPEMNPSTITQKAEQVNSSKTALNGDENLAAAKQNAKTYLNTLTSITDAQK
                     NNLISQITSATRVSGVDTVKQNAQHLDQAMASLQNGINNESQVKSSEKYRDADTNKQQ
                     EYDNAITAAKAILNKSTGPNTAQNAVEAALQRVNNAKDALNGDAKLIAAQNAAKQHLG
                     TLTHITTAQRNDLTNQISQATNLAGVESVKQNANSLDGAMGNLQTAINDKSGTLASQN
                     FLDADEQKRNAYNQAVSAAETILNKQTGPNTAKTAVEQALNNVNNAKHALNGTQNLNN
                     AKQAAITAINGASDLNQKQKDALKAQANGAQRVSNAQDVQHNATELNTAMGTLKHAIA
                     DKTNTLASSKYVNADSTKQNAYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQ
                     ELNGDERLREAKQNANTAIDALTQLNTPQKAKLKEQVGQANRLEDVQTVQTNGQALNN
                     AMKGLRDSIANETTVKTSQNYTDASPNNQSTYNSAVSNAKGIINQTNNPTMDTSAITQ
                     ATTQVNNAKNGLNGAENLRNAQNTAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTA
                     TKNAATELNTQMGNLEQAIHDQNTVKQSVKFTDADKAKRDAYTNAVSRAEAILNKTQG
                     ANTSKQDVEAAIQNVSSAKNALNGDQNVTNAKNAAKNALNNLTSINNAQKRDLTTKID
                     QATTVAGVEAVSNTSTQLNTAMANLQNGINDKTNTLASENYHDADSDKKTAYTQAVTN
                     AENILNKNSGSNLDKTAVENALSQVANAKGALNGNHNLEQAKSNANTTINGLQHLTTA
                     QKDKLKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNTATKNGQNYLDATERN
                     KTNYNNAVDSANGVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATNA
                     IDGATNLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQT
                     QKYRDAEQSKKTAYDQAVAAAKAILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKL
                     AEAKAAAKQNLGTLNHITNAQRTDLEGQINQATTVDGVNTVKTNANTLDGAMNSLQGS
                     INDKDATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRA
                     KAALNGADNLRNAKTSATNTIDGLPNLTQLQKDNLKHQVEQAQNVAGVNGVKDKGNTL
                     NTAMGALRTSIQNDNTTKTSQNYLDASDSNKNNYNTAVNNANGVINATNNPNMDANAI
                     NGMANQVNTTKAALNGAQNLAQAKTNATNTINNAHDLNQKQKDALKTQVNNAQRVSDA
                     NNVQHTATELNSAMTALKAAIADKERTKASGNYVNADQEKRQAYDSKVTNAENIISGT
                     PNATLTVNDVNSAASQVNAAKTALNGDNNLRVAKEHANNTIDGLAQLNNAQKAKLKEQ
                     VQSATTLDGVQTVKNSSQTLNTAMKGLRDSIANEATIKAGQNYTDASPNNRNEYDSAV
                     TAAKAIINQTSNPTMEPNTITQVTSQVTTKEQALNGARNLAQAKTTAKNNLNNLTSIN
                     NAQKDALTRSIDGATTVAGVNQETAKATELNNAMHSLQNGINDETQTKQTQKYLDAEP
                     SKKSAYDQAVNAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAK
                     QTLGTLTHINNAQRTALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTL
                     QSQNYQDADDAKRTAYSQAVNAAATILNKTAGGNTPKADVERAMQAVTQANTALNGIQ
                     NLDRAKQAANTAITNASDLNTKQKEALKAQVTSAGRVSAANGVEHTATELNTAMTALK
                     RAIADKAETKASGNYVNADANKRQAYDEKVTAAENIVSGTPTPTLTPADVTNAATQVT
                     NAKTQLNGNHNLEVAKQNANTAIDGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQ
                     TLNTAMKGLRDSIANEATIKAGQNYTDASQNKQTDYNSAVTAAKAIIGQTTSPSMNAQ
                     EINQAKDQVTAKQQALNGQENLRTAQTNAKQHLNGLSDLTDAQKDAVKRQIEGATHVN
                     EVTQAQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILN
                     KAQGPNTSKDGVETALENVQRAKNELNGNQNVANAKTTAKNALNNLTSINNAQKEALK
                     SQIEGATTVAGVNQVSTTASELNTAMSNLQNGINDEAATKAAQKYTDADREKQTAYND
                     AVTAAKTLLDKTAGSNDNKAAVEQALQRVNTAKTALNGDERLNEAKNTAKQQVATMSH
                     LTDAQKANLTSQIESGTTVAGVQGIQANAGTLDQAMNQLRQSIASKDATKSSEDYQDA
                     NADLQNAYNDAVTNAEGIISATNNPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQT
                     AKSDIGRLTDLNNAQRTAANAEVDQAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDN
                     TKRSVNYTDADQPKQQAYDTAVTQAEAITNANGSNANETQVQAALNQLNQAKNDLNGD
                     NKVAQAKESAKRALASYSNLNNAQSTAAISQIDNATTVAGVTAAQNTANELNTAMGQL
                     QNGINDQNTVKQQVNFTDADQGKKDAYTNAVTNAQGILDKAHGQNMTKAQVEAALNQV
                     TTAKNALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIEGATTVNGVNGVKTKA
                     QDLDGAMQRLQSAIANKDQTKASENYIDADPTKKTAFDNAITQAESYLNKDHGANKDK
                     QAVEQAIQSVTSTENALNGDANLQRAKTEAIQAIDNLTHLNTPQKTALKQQVNAAQRV
                     SGVTDLKNSATSLNNAMDQLKQAIADHDTIVASGNYTNASPDKQGAYTDAYNAAKNIV
                     NGSPNVITNAADVTAATQRVNNAETGLNGDTNLATAKQQAKDALRQMTHLSDAQKQSI
                     TGQIDSATQVTGVQSVKDNATNLDNAMNQLRNSIANKDDVKASQPYVDADRDKQNAYN
                     TAVTNAENIINATSQPTLDPSAVTQAANQVSTNKTALNGAQNLANKKQETTANINQLS
                     HLNNAQKQDLNTQVTNAPNISTVNQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTD
                     ADPEKQTAYNNAVTAAEVTQAIQTAQELNTAMGNLKNSLNDKDTTLGSQNFADADPEK
                     KNAYNEAVHNAENILNKSTGTNVPKDQVEAAMNQVNATKAALNGTQNLEKAKQHANTA
                     IDGLSHLTNAQKEALKQLVQQSTTVAEAQGNEQKANNVDAAMDKLRQSIADNATTKQN
                     QNYTDASQNKKDAYNNAVTTAQGIIDQTTSPTLDPTVINQAAGQVSTTKNALNGNENL
                     EAAKQQASQSLGSLDNLNNAQKQTVTDQINGAHTVDEANQIKQNAQNLNTAMGNLKQA
                     IADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNATQAEVEQAIKQVNAA
                     KQALNGNANVQHAKDEATALINSSNDLNQAQKDALKQQVQNATTVAGVNNVKQTAQEL
                     NNAMTQLKQGIADKEQTKADGNFVNADPDKQNAYNQAVAKAEALISATPDVVVTPSEI
                     TAALNKVTQAKNDLNGNTNLATAKQNVQHAIDQLPNLNQAQRDEYSKQITQATLVPNV
                     NAIQQAATTLNDAMTQLKQGIANKAQIKGSENYHDADTDKQTAYDNAVTKAEELLKQT
                     TNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQDAKTTLGTLDHLNDAQKQALTTQ
                     VEQAPDIATVNNVKQNAQNLNNAMTNLNNALQDKTETLNSINFTDADQAKKDAYTNAV
                     SHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQRAKDAAKQVITNANDLN
                     QAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIADKDQTKANGNFVNADT
                     DKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVNQAKGDLNGNHNLQVAKDNAN
                     TAIDQLPNLNQPQKTALKDQVSHAELVTGVNAIKQNADALNNAMGTLKQQIQANSQVP
                     QSVDFTQADQDKQQAYNNAANQAQQIANGIPTPVLTPDTVTQAVTTMNQAKDALNGDE
                     KLAQAKQEALANLDTLRDLNQPQRDALRNQINQAQALATVEQTKQNAQNVNTAMSNLK
                     QGIANKDTVKASENYHDADADKQTAYTNAVSQAEGIINQTTNPTLNPDEITRALTQVT
                     DAKNGLNGEAKLATEKQNAKDAVSGMTHLNDAQKQALKGQIDQSPEIATVNQVKQTAT
                     SLDQAMDQLSQAINDKAQTLADGNYLNADPDKQNAYKQAVAKAEALLNKQSGTNEVQA
                     QVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQKQSFESQITQAPLVT
                     DVTTINQKAQTLDHAMELLRNSVADNQTTLASEDYHDATAQRQNDYNQAVTAANNIIN
                     QTTSPTMNPDDVNGATTQVNNTKVALDGDENLAAAKQQANNRLDQLDHLNNAQKQQLQ
                     SQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQAVEQRGNFINADTDKQTAYNT
                     AVNEAAAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQVAKTNATQAIDALTS
                     LNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRQSIQDNAATKANSKYINE
                     DQPEQQNYDQAVQAANNIINEQTATLDNNAINQAATTVNTTKAALHGDVKLQNDKDHA
                     KQTVSQLAHLNNAQKHMEDTLIDSETTRTAVKQDLTEAQALDQLMDALQQSIADKDAT
                     RASSAYVNAEPNKKQSYDEAVQNAESIIAGLNNPTINKGNVSSATQAVISSKNALDGV
                     ERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRGEVAQKLTEAQALNQAMEAL
                     RNSIQDQQQTEAGSKFINEDKPQKDAYQAAVQNAKDLINQTNNPTLDKAQVEQLTQAV
                     NQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNPQRQALESQINNAATRGEVAQKLAEA
                     KALDQAMQALRNSIQDQQQTESGSKFINEDKPQKDAYQAAVQNAKDLINQTGNPTLDK
                     SQVEQLTQAVTTAKDNLHGDQKLARDQQQAVTTVNALPNLNHAQQQALTDAINAAPTR
                     TEVAQHVQTATELDHAMETLKNKVDQVNTDKAQPNYTEASTDKKEAVDQALQAAESIT
                     DPTNASNANKDAVDQVLTKLQEKENELNGNERVAEAKTQAKQTIDQLTHLNADQIATA
                     KQNIDQATKLQPIAELVDQATQLNQSMDQLQQAVNEHANVEQTVDYTQADSDKQNAYK
                     QAIADAENVLKQNANKQQVDQALQNILNAKQALNGDERVALAKTNGKHDIDQLNALNN
                     AQQDGFKGRIDQSNDLNQIQQIVDEAKALNRAMDQLSQEITDNEGRTKGSTNYVNADT
                     QVKQVYDETVDKAKQALDKSTGQNLTAKQVIKLNDAVTAAKKALNGEERLNNRKAEAL
                     QRLDQLTHLNNAQRQLAIQQINNAETLNKASRAINRATKLDNAMGAVQQYIDEQHLGV
                     ISSTNYINADDNLKANYDNAIANAAHELDKVQGNAIAKAEAEQLKQNIIDAQNALNGD
                     QNLANAKDKANAFVNSLNGLNQQQQDLAHKAINNADTVSDVTDIVNNQIDLNDAMETL
                     KHLVDNEIPNAEQTVNYQNADDNAKTNFDDAKRLANTLLNSDNTNVNDINGAIQAVND
                     AIHNLNGDQRLQDAKDKAIQSINQALANKLKEIEASNATDQDKLIAKNKAEELANSII
                     NNINKATSNQAVSQVQTAGNHAIEQVHANEIPKAKIDANKDVDKQVQALIDEIDRNPN
                     LTDKEKQALKDRINQILQQGHNGINNAMTKEEIEQAKAQLAQALQDIKDLVKAKEDAK
                     QDVDKQVQALIDEIDQNPNLTDKEKQALKYRINQILQQGHNDINNALTKEEIEQAKAQ
                     LAQALQDIKDLVKAKEDAKNAIKALANAKRDQINSNPDLTPEQKAKALKEIDEAEKRA
                     LQNVENAQTIDQLNRGLNLGLDDIRNTHVWEVDEQPAVNEIFEATPEQILVNGELIVH
                     RDDIITEQDILAHINLIDQLSAEVIDTPSTATISDSLTAKVEVTLLDGSKVIVNVPVK
                     VVEKELSVVKQQAIESIENAAQQKINEINNSVTLTLEQKEAAIAEVNKLKQQAIDHVN
                     NAPDVHSVEEIQQQEQAHIEQFNPEQFTIEQAKSNAIKSIEDAIQHMIDEIKARTDLT
                     DKEKQEAIAKLNQLKEQAIQAIQRAQSIDEISEQLEQFKAQMKAANPTAKELAKRKQE
                     AISRIKDFSNEKINSIRNSEIGTADEKQAAMNQINEIVLETIRDINNAHTLQQVEAAL
                     NNGIARISAVQIVTSDRAKQSSSTGNESNSHLTIGYGTANHPFNSSTIGHKKKLDEDD
                     DIDPLHMRHFSNNFGNVIKNAIGVVGISGLLASFWFFIAKRRRKEDEEEELEIRDNNK
                     DSIKETLDDTKHLPLLFAKRRRKEDEEDVEVTNENTDEKVLKDNEHSPLLFAKRRKDK
                     EEDVETTTSIESKDEDVPLLLAKKKNQKDNQSKDKKSASKNTSKKVAAKKKKKKAKKN
                     KK"
     gene            complement(36609..37010)
                     /locus_tag="O2692_000649"
     CDS             complement(36609..37010)
                     /locus_tag="O2692_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCZ4298290.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKLIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
     gene            complement(37551..38255)
                     /locus_tag="O2692_000650"
     CDS             complement(37551..38255)
                     /locus_tag="O2692_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HCZ4298291.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            38500..38694
                     /locus_tag="O2692_000651"
     CDS             38500..38694
                     /locus_tag="O2692_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HCZ4298292.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            38706..38957
                     /locus_tag="O2692_000652"
     CDS             38706..38957
                     /locus_tag="O2692_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HCZ4298293.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            38995..40119
                     /locus_tag="O2692_000653"
     CDS             38995..40119
                     /locus_tag="O2692_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HCZ4298294.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIQEVMDGVLEEVLTTYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPVVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            40135..40572
                     /locus_tag="O2692_000654"
     CDS             40135..40572
                     /locus_tag="O2692_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCZ4298295.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
BASE COUNT        16013 a         7029 c         7365 g        10259 t
ORIGIN      
        1 cataaccaaa ggataaaata attgtgaaat tattcacaaa tgaaagagga gtgttgcaaa
       61 tgttagttgc agtagtcaaa gaattaaaac aaggtgaagg acgtgtagct tgcacacccg
      121 aaaatgtgcg taagttaacg gatgcaggac ataaagtaat tgttgaaaaa aatgctggca
      181 ttggttcagg attttctaac gatatgtatg aaaaagaagg cgctaagatc gtaactcacg
      241 aacaagcatg ggaagctgat cttgttatca aagtaaaaga acctcatgaa agcgaatatc
      301 aatatttcaa aaagaatcaa attatctggg gatttttaca tctagcatct tcaaaagaaa
      361 tagtagaaaa aatgcaagaa gttggtgtaa ctgcgattag tggtgaaacc attataaaaa
      421 atggaaaagc agaattatta gcgccaatga gtgctatagc aggtcaacgc tcagcaatta
      481 tgggagctta ctactctgaa gcacaacatg gtggtcaagg tactttagtg actggtgtac
      541 atgaaaatgt ggatatacct ggtagtacat atgtgatttt cggtggtgga gtagcagcaa
      601 caaatgcagc aaatgttgcc ttgggactaa atgctaaagt aatcattatc gagttaaacg
      661 atgaccgcat taaatatctt gaagatatgt atgcagaaaa agatgtcaca gtagtcaaat
      721 caacaccaga aaatttagca gaacaaatta agaaagcaga tgtatttatt tctacaattt
      781 taattccagg tgcgaaaccg ccaaaattgg ttactcgtga gatggttaaa tcaatgaaaa
      841 aaggttcagt attaatcgat atagctattg accaaggtgg aactattgaa acaattagac
      901 caactacaat ttctgatcca gtgtatgaag aagaaggtgt gattcattat ggtgtaccaa
      961 atcaaccagg agcagtccca agaacttcaa caatggcatt agcacaagga aatattgatt
     1021 atatattaga aatttgtgac aaaggcttag aacaagcaat taaagataat gaagccttaa
     1081 gtactggtgt aaacatttac caaggacaag tgacaaatca aggattagct tcatcacatg
     1141 acctagatta taaagaaata ttaaatgtta tcgaatagat agtaatttaa atgaaattga
     1201 gtgaaatgaa tattttaaat atagcattat agtttggact aaagatttac aaaacggaag
     1261 gatgtaatta ttatgacaac caacacagtt acattacaaa cagcacatat tgtaagttta
     1321 ggagatatcg aagaagctaa agcaagcatt aaaccattta ttcgtcgaac acctctaatt
     1381 aaatcaatgt atttaagcca aagtataact aaagggaatg tatttctaaa attagaaaat
     1441 atgcaattca caggatcttt taaatttaga ggcgctagca ataaaattaa tcacttaaca
     1501 gatgaacaaa aagaaaaagg cattatcgca gcatctgctg ggaaccatgc acaaggtgtt
     1561 gctttaacag ctaaattatt aggcattgat gcaacgattg taatgcctga aacagcacca
     1621 caagcgaaac aacaagcaac aaaaggctat ggggcaaagg ttattttaaa aggtaaaaac
     1681 tttaacgaaa ctagacttta tatggaagaa ttagcgaaag aaaatggcat gacaatcgtt
     1741 catccatatg acgataagtt tgtaatggca ggccaaggaa caattggttt agaaatttta
     1801 gatgatattt ggaatgtgaa tacagtcatc gtaccagttg gcggtggagg attaattgca
     1861 ggtattgcca ccgcattaaa atcatttaac ccttcaattc atattatcgg tgttcaatct
     1921 gagaatgttc atggtatggc tgagtctttc tataagagag atttaactga acatcgagtg
     1981 gatagcacaa tagcagatgg ttgtgatgta aaagttcctg gtgaacaaac atatgaagta
     2041 gttaaacatt tagtagatga atttattctt gttactgaag aagaaattga acatgctatg
     2101 aaagatttaa tgcagcgtgc caaaattatt actgaaggtg caggcgcatt accaacagct
     2161 gcaattttaa gtggaaaaat aaacaataaa tggcttgaag ataaaaatgt tgttgcatta
     2221 gtttcaggcg ggaatgttga cttaactaga gtttcaggtg tcattgaaca tggactgaat
     2281 attgcagata caagcaaggg tgtggtaggt taaaacattt aatcttaaaa atgaggtgta
     2341 attatgtcaa atggtaaaga attacaaaaa aatataggtt tcttctcagc gtttgctatt
     2401 gttatgggga cagttattgg ttcaggagta ttctttaaaa tatcaaacgt aacagaagta
     2461 acaggaacag caggaatggc cttgtttgta tggttcctag gcggcatcat taccatttgt
     2521 gcggggttaa cagcagcaga acttgctgct gcaatccctg aaacaggtgg cttaacgaag
     2581 tatatagaat atacatacgg tgatttctgg ggcttcctat caggttgggc gcaatcattt
     2641 atttattttc cagctaacgt agcagcattg tctatcgtat ttgcgacaca gctaattaat
     2701 ttattccatt tatctatagg ttcgttaata ccaatagcaa tcgcatctgc gttatctatt
     2761 gtgttgataa atttcctagg ttcaaaagca ggcggaattt tacaatcagt tactttagta
     2821 attaaactga ttccaatcat cgttattgta atttttggta tttttcaatc tggagatatc
     2881 actttttcat taattccaac tacaggtaat tcaggaaatg gcttctttac agcaattggt
     2941 agtggtttat tagcaactat gtttgcatat gatggttgga ttcatgtagg aaatgttgcg
     3001 ggggaactta aaaatcctaa acgcgattta cctttagcga tttcagttgg tatcggttgt
     3061 attatggctg tgtatttatt aattaacgct acattcttat taacgttgcc aatagaacta
     3121 cttgctggta atttaaatgc agcttcagat acatcaaaaa tattatttgg tgaaaatggc
     3181 ggtaagatta ttacaatcgg tatattaatt tctgtttatg gtacgatcaa tggctatact
     3241 atgactggta tgcgcgtacc atatgcaatg gctgaaagaa aattattgcc atttagccat
     3301 ttattcgcaa aattaacaaa atctggcgca ccatggtttg gcgcaattat acaacttata
     3361 atcgctatca tcatgatgtc aatgggagca tttgatacaa ttacaaatat gttaatcttt
     3421 gttatttggt tgttctattg tatgtcattt gttgcggtaa taattttaag aaaacgtgaa
     3481 ccaaatatgg aacgaccata taaagtaccg ttatatccga tcataccttt aattgctatt
     3541 ttggcaggat catttgtatt aattaataca ctgtttacac aatttatatt agcaatcatt
     3601 ggaattctaa taacagcact tggtatacca gtttattact ataaaaagaa acaaaaagca
     3661 gcataaggta agataactag cattgagaat aaatggatgg actactaata aatttaaagt
     3721 tttacacatt aaaatcaaaa accattcaat tattctatgg aacagacaaa tttctgttat
     3781 ggaatttgtc tgtttttcaa aagtataggg aggcaaatag agatggaaaa gccgtcaaga
     3841 gaggcatttg aaggcaataa taagttgtta ataggaattg ttctaagtgt aataacgttt
     3901 tggctatttg cacaatcatt ggttaatgtt gtaccaatac ttgaagatag tttcaataca
     3961 gatattggaa cggttaatat cgccgttagt ataactgctt tattttcagg aatgtttgta
     4021 gtaggagcag gtggtcttgc tgataaatat ggcagaatta aactcacgaa cattggtatt
     4081 atcttaaata tattaggttc attattaatc attatttcaa atattccttt attacttatt
     4141 ataggaagat taattcaagg actttcagca gcatgtatta tgcctgcaac tttgtctatt
     4201 attaagtcat attacattgg gaaagataga caacgcgctt taagttattg gtcaattggc
     4261 tcatggggcg gctctggtgt ttgttcattt tttggaggtg cagttgcaac gcttttaggt
     4321 tggcgttgga ttttcatcct atcaattata atttcattaa ttgcactgtt tcttattaaa
     4381 ggcacacctg aaactaaatc taaatcgatt tctctaaata aatttgacat taaaggtctg
     4441 gttcttttag tcattatgct cctcagttta aatattttaa ttactaaagg atcagaatta
     4501 ggtgtaacct cacttctttt tattacttta ttagctattg caattggatc ttttagttta
     4561 tttatagttc ttgaaaagcg tgctacaaat cctttaatcg attttaaatt atttaaaaat
     4621 aaagcttaca caggtgcaac agcttcaaac tttttgttaa atggtgttgc aggaacatta
     4681 atagtagcca acacatttgt tcaaagaggt ttaggatatt cttcattgca agcaggaagt
     4741 ttatcaatca cttatttagt aatggtacta attatgattc gtgttggtga aaagttactt
     4801 caaacactcg gatgcaagaa accaatgtta attggaacag gagttcttat tgtcggagaa
     4861 tgtctcattt cattaacttt cttgccagaa atattctatg tcatttgttg tattataggt
     4921 tatttattct ttggtttagg actagggata tatgctacac catcaacaga tacagcaatt
     4981 gcaaatgcac cgttagaaaa agtaggcgtt gctgcaggta tctataaaat ggcttctgca
     5041 ttaggtggag catttggcgt cgcattgagt ggtgcagtat atgcaatcgt atcaaatatg
     5101 acaaacattt atacaggtgc aatgattgca ttatggttaa atgcaggtat gggaatatta
     5161 tcattcgtta tcattttgtt acttgtgcct aaacaaaacg atactcaatt atgataattg
     5221 agaattaaat tgaaatcata caagtcgcta caatattaaa caaaaatata aaccgattct
     5281 tatgtgtcat tattttaaat gaacataggg attggttttt tattactctt ttacgctact
     5341 ttatttataa ttattataaa ttgtcacaaa ttcaatttac cttacaatat attttgtgtt
     5401 attatattct ggagcataaa taaattgttc aacacatagt tgtaatgtgt ttcaatactt
     5461 tttggataga ttgcgaaatt gtattgaatc gtcatcgttt taaattttta aatgagaatg
     5521 gaatgagcat tacaatacac aagcaatcaa aagtaaatac attcacaaca caacagagac
     5581 ataacaacaa gataaggagt gaacaatagc tgtgaattat cgtgataaaa ttcaaaagtt
     5641 tagtattcgt aaatatacag ttggtacatt ttcaactgtc attgcgacat tggtattttt
     5701 aggattcaat acatcacaag cacatgctgc tgaaacaaat caaccagcaa gcgtggttaa
     5761 acagaaacaa caaagtaata atgaacagac tgagaatcga gaatctcaag tacaaaattc
     5821 tcaaaattca caaaatggtc aatcattatc tgctactcat gaaaatgagc aaccaaatat
     5881 tagtcaagct aatttagtag atcaaaaagt agcgcaatca tctactacta atgatgaaca
     5941 accagcatct caaaatgtaa atacaaagaa agattcggca acggctgcga caacacaacc
     6001 agataaagaa caaagtaagc ataaacaaaa cgaaagtcaa tctgctaata aaaatggaaa
     6061 cgacaataga gcggctcatg tagaaaatca tgaagcaaat gtagtaacag cttcagattc
     6121 atctgataat ggtaacgtac aacatgaccg aaatgaatta caagcgtttt ttgatgcaaa
     6181 ttatcatgat tatcgcttta ttgaccgtga aaatgcagat tctggcacat ttaactatgt
     6241 aaaaggcatt tttgataaga ttaatacgtt attaggcagt aatgatccaa taaacaataa
     6301 agacttgcaa cttgcataca aagaattgga acaagctgtt gctttaattc gtacaatgcc
     6361 tcaacgtcaa cagactagcc gacgttcaaa tagaattcaa acgcgttcgg ttgagtcaag
     6421 agctgcagag cctagatcag tatcagacta tcaaaatgca aattcatcat attatgttga
     6481 aaatgctaat gatggttcgg gctatcctgt tggtacatat atcaatgctt ctagtaaagg
     6541 ggcgccatat aatttaccaa ctacaccatg gaatacattg aaggcctctg actcaaagga
     6601 aattgctctt atgacagcga aacaaactgg agacgggtac caatgggtta ttaagtttaa
     6661 taaaggacat gctccacatc aaaatatgat cttttggttt gcattaccag cagaccaagt
     6721 gccagtagga agaactgact ttgtaacagt taattcagat ggaacaaatg tacaatggag
     6781 tcatggagca ggagcaggtg caaataaacc acttcaacaa atgtgggaat atggagtaaa
     6841 tgatcctcat cgttcacatg actttaaaat aagaaataga agtggccaag taatatatga
     6901 ctggccaact gtccatattt attctttaga agatttatct agagcgagtg attattttag
     6961 tgaagctgga gcgacacctg ctactaaagc ttttggtaga caaaattttg aatatattaa
     7021 tggtcaaaaa cctgctgaat caccgggtgt tcctaaagtt tatactttca tcggtcaagg
     7081 tgatgcaagt tatacaattt catttaaaac acaaggtcca actgttaata aattgtacta
     7141 tgcagcaggt gggcgtgctt tagagtacaa tcaattattt atgtacagtc aactatacgt
     7201 cgaatcaacg caagaccatc aacaacgtct taatggttta agacaagtgg ttaatcgtac
     7261 atatcgcata ggtacaacta aacgtgtaga agtgagtcaa ggaaatgtac aaacgaaaaa
     7321 ggtattagaa agtacaaacc taaatataga tgattttgtt gatgatcctt taagttatgt
     7381 taagacgccg agtaataaag tgttaggatt ttattcgaat aatgcaaata ctaatgcttt
     7441 tagaccgggt ggagcccaac aattaaatga atatcaatta agtcaattat ttactgatca
     7501 aaaattacaa gaagcagcaa gaactagaaa cccaataaga ttaatgattg gtttcgacta
     7561 tcctgatgct tatggtaata gtgaaacttt agttcctgtt aacttaacgg tattacctga
     7621 aatccaacat aatattaaat tctttaaaaa tgacgatact caaaatattg ctgaaaaacc
     7681 attttcaaaa caagctgggc atccagtttt ctatgtatat gcaggtaacc aagggaatgc
     7741 ttccgtgaat ttaggtggta gcgtaacatc tattcaacca ttacgtatta atttaacaag
     7801 taatgagaat tttacagata aagattggca aattacaggt attccgcgta cattacacat
     7861 tgaaaactcg acaaatagac ctaataatgc cagagaacgc aatattgaac ttgttggtaa
     7921 cttattacca ggggattact ttggaacgat acgttttgga cgtaaagaac aattattcga
     7981 aattcgtgtt aaaccacata caccaacaat tacaacgaca gctgagcaat taagaggtac
     8041 agcattacaa aaagtgcctg ttaatatttc gggaataccg ttggatccat cggcattggt
     8101 ttatttagtt gcaccaacaa atcaaactac gaatggtggt agtgaggcag atcaaatacc
     8161 atctggttat acgatacttg cgactggtac acctgatggg gtgcataata caattactat
     8221 acgaccgcaa gattatgttg tattcatacc acctgtaggt aaacaaatta gagcagtagt
     8281 ttattataat aaagtagttg catctaatat gagtaatgct gttactattt tgccagatga
     8341 cattccacca acaatcaata atcctgttgg aataaatgcc aaatactatc gaggcgacga
     8401 agtcaacttt acaatgggtg tctctgatag acattctggt ataaaaaata caactattac
     8461 gacattgccg aatggttgga catcaaattt aacaaaagcg gataagaata atggctcatt
     8521 atctattaca ggtagagtga gtatgaatca ggcatttaac agtgatatta catttaaagt
     8581 gtcagcgaca gacaatgtca ataatacgac aaatgatagt caatctaaac atgtttcaat
     8641 tcatgtaggt aaaattagtg aagatgctca tccgattgta ttaggaaata ctgagaaagt
     8701 tgtagtagtc aatccgactg ctgtatctaa tgatgaaaag caaagcataa ttactgcctt
     8761 tatgaataaa aaccaaaata taagaggata tttagcatca actgatccag taactgtcga
     8821 taataatggt aatgtcacat tacattaccg tgatggctca tcgacaacgc ttgatgctac
     8881 aaatgtgatg acatacgaac cagttgtgaa acctgaatac caaactgtca atgctgctaa
     8941 aacagcaacg gtaacgattg ctaaaggaca atcatttagt attggtgata ttaaacaata
     9001 ttttacttta agtaatggac aacctattcc aagtggcaca tttacaaata ttacatctga
     9061 tagaactatt ccaactgcac aagaagttag tcaaatgaac gcaggcacgc agttatacca
     9121 tataactgct acaaatgcgt atcataaaga tagtgaagac ttctatatta gtttgaaaat
     9181 catcgatgtg aaacaaccag aaggcgatca acgtgtatat cgtacatcaa catatgattt
     9241 aactactgat gaaatctcaa aagtaaaaca agcatttatt aatgcaaata gagatgtaat
     9301 tacgcttgcc gaaggtgata tttcagttac aaatacacct aatggtgcta atgtaagtac
     9361 tattacagta aatattaata aaggtcgatt aacgaaatca ttcgcgtcaa acctagctaa
     9421 tatgaatttc ttgcgttggg ttaatttccc acaagattat acagtgacat ggacgaatgc
     9481 aaaaattgca aacagaccaa cagatggtgg tttatcatgg tctgatgacc ataaatcttt
     9541 aatttatcgt tatgatgcta cattaggtac tcaaattacg acgaatgata ttttaacaat
     9601 gttaaaagca acaactacag tgcctggatt gcgaaataac attactggta atgaaaaatc
     9661 acaagcagaa gctggcggaa gacctaactt tagaacgact ggttattcac aatcaaatgc
     9721 gacaactgat ggtcaacgtc aatttacgtt gaatggtcaa gtgattcaag tgttagacat
     9781 catcaaccct tcaaacggtt atggtgggca acctgttaca aattcaaata ctcgtgcaaa
     9841 ccatagtaac tcaactgttg ttaacgtaaa cgaaccggca gctaatggtg ctggcgcatt
     9901 tacaattgac cacgttgtaa aaagtaattc tacacataat gcaagtgatg cagtttataa
     9961 agcacagtta tacttaacgc catatggtcc aaaacaatat gttgaacatt taaatcaaaa
    10021 tacaggaaat actactgacg ctattaacat ttattttgta ccaagtgact tagtgaatcc
    10081 aacaatttca gtaggtaatt acactaatca tcaagtgttc tcaggtgaaa catttacaaa
    10141 tactattaca gcgaatgata actttggtgt gcaatctgta actgtaccaa atacatcaca
    10201 aattacaggt actgttgata ataaccatca acatgtttct gcaacggcac caaatgtgac
    10261 atcagcaact aataagacaa tcaatttatt agcaactgat acaagcggca atacagctac
    10321 aacttcgttc aatgtaacag tgaaaccttt gcgtgataaa tatcgagttg gtacttcatc
    10381 aacggctgct aatcctgtga gaattgccaa tatttcgaat aatgcgacag tatcacaagc
    10441 tgatcaaacg acaattatta attcgttaac gtttactgaa acagtaccaa atagaagtta
    10501 tgcaagagca agtgcgaatg aaatcactag taaaacagtt agtaatgtca gtcgtactgg
    10561 aaataatgcc aatgtcacag taactgttac ttatcaagat ggaacaacat caacagtgac
    10621 tgtacctgta aagcatgtca ttccagaaat cgttgcacat tcgcattaca ctgtacaagg
    10681 ccaagacttc ccagcaggta atggttctag tgcatcagat tactttaagt tatctaatgg
    10741 tagtgacatt gcagatgcaa ctattacatg ggtaagtgga caagcgccaa ataaagataa
    10801 tacacgtatt ggtgaagata taactgtaac tgcacatatc ttaattgatg gcgaaacaac
    10861 gccgattacg aaaacagcaa catataaagt agtaagaact gtaccgaaac atgtctttga
    10921 aacagccaga ggtgttttat acccaggtgt ttcagatatg tatgatgcga aacaatatgt
    10981 taagccagta aataattctt ggtcgacaaa tgcgcaacat atgaatttcc aatttgttgg
    11041 aacatatggt cctaacaaag atgttgtagg catatctact cgtcttatta gagtgacata
    11101 tgataataga caaacagaag atttaactat tttatctaaa gttaaacctg acccacctag
    11161 aattgacgca aactctgtga catataaagc aggtcttaca aaccaagaaa ttaaagttaa
    11221 taacgtatta aataactcgt cagtaaaatt atttaaagca gataatacac cattaaatgt
    11281 cacaaatatt actcatggta gcggttttag ttcggttgtg acagtaagtg acgcgttacc
    11341 aaatggcgga attaaagcaa aatcttcaat ttcaatgaac aatgtgacgt atacgacgca
    11401 agacgaacat ggtcaagttg ttacagtaac aagaaatgaa tctgttgatt caaatgacag
    11461 tgcaacagta acagtgacac cacaattaca agcaactact gaaggcgctg tatttattaa
    11521 aggtggcgac ggttttgatt tcggacacgt agaaagattt attcaaaacc cgccacatgg
    11581 ggcaacggtt gcatggcatg atagtccaga tacatggaag aatacagtcg gtaacactca
    11641 taaaactgcg gttgtaacat tacctaatgg tcaaggtacg cgtaatgttg aagttccagt
    11701 caaagtttat ccagttgcta atgcaaaggc gccatcacgt gatgtgaaag gtcaaaattt
    11761 gactaatgga acggatgcga tgaactacat tacatttgat ccaaatacaa acacaaatgg
    11821 tatcactgca gcatgggcaa atagacaaca accaaataac caacaagcag gcgtgcaaca
    11881 tttaaatgtc gatgtcacat atccaggtat ttcagctgct aaacgagttc ctgttactgt
    11941 taatgtatat caatttgaat tccctcaaac tacttatacg acaacggttg gaggcacttt
    12001 agcaagtggt acgcaagcat caggatatgc acatatgcaa aatgctactg gtttaccaac
    12061 agatggattt acgtataaat ggaatcgtga tactacaggt acaaatgacg caaactggtc
    12121 agctatgaat aaaccgaatg tggctaaagt cgttaacgca aaatatgacg tcatctataa
    12181 cggacatact tttgcaacat ctttaccagc gaaatttgta gtaaaagatg tgcaaccagc
    12241 gaaaccaact gtgactgaaa cagcggcagg agcgattaca attgcacctg gagcaaacca
    12301 aacagtgaat acacatgccg gtaacgtaac gacatacgct gataaattag ttattaaacg
    12361 taatggtaac gttgtgacga catttacacg tcgcaataat acgagtccat gggtgaaaga
    12421 agcatctgca gcaactgtag caggtattgc tggaactaat aatggtatta ctgttgcagc
    12481 aggtactttc aaccctgctg atacaattca agttgttgca acgcaaggaa gcggagagac
    12541 agtgagtgat gagcaacgta gtgatgattt cacagttgtc gcaccacaac cgaaccaagc
    12601 gactactaag atttggcaaa atggtcatat tgatatcacg cctaataatc catcaggaca
    12661 tttaattaat ccaactcaag caatggatat tgcttacact gaaaaagtgg gtaatggtgc
    12721 agaacatagt aagacaatta atgttgttcg tggtcaaaat aatcaatgga caattgcgaa
    12781 taagcctgac tatgtaacgt tagatgcaca aactggtaaa gtgacgttca atgccaatac
    12841 tataaaacca aattcatcaa tcacaattac tccgaaagca ggtacaggtc actcagtaag
    12901 tagtaatcca agtacattaa ctgcaccggc agctcatact gtcaacacaa ctgaaattgt
    12961 gaaagattat ggttcaaatg taacagcagc tgaaattaac aatgcagttc aagttgctaa
    13021 taaacgtact gcaacgatta aaaatggcac agcaatgcct actaatttag ctggtggtag
    13081 cacaacgacg attcctgtga cagtaactta caatgatggt agtactgaag aagtacaaga
    13141 gtccattttc acaaaagcgg ataaacgtga gttaatcaca gctaaaaatc atttagatga
    13201 tccagtaagc actgaaggta aaaagccagg tacaattacg cagtacaata atgcaatgca
    13261 taatgcgcaa caacaaatca atactgcgaa aacagaagca caacaagtga ttaataatga
    13321 gcgtgcaaca ccacaacaag tttctgacgc actaactaaa gttcgtgcag cacaaactaa
    13381 gattgatcaa gctaaagcat tacttcaaaa taaagaagat aatagccaat tagtaacgtc
    13441 taaaaataac ttacaaagtt ctgtgaacca agtaccatca actgctggta tgacgcaaca
    13501 aagtattgat aactataatg cgaagaagcg tgaagcagaa actgaaataa ctgcagctca
    13561 acgtgttatt gacaatggcg atgcaactgc acaacaaatt tcagatgaaa aacatcgtgt
    13621 cgataacgca ttaacagcat taaaccaagc gaaacatgat ttaactgcag atacacatgc
    13681 cttagagcaa gcagtgcaac aattgaatcg cacaggtaca acgactggta agaagccggc
    13741 aagtattact gcttacaata attcgattcg tgcacttcaa agtgacttaa caagtgctaa
    13801 aaatagcgct aatgctatta ttcaaaagcc aataagaaca gtacaagaag tgcaatctgc
    13861 gttaacaaat gtaaatcgtg tcaatgagcg attaacgcaa gcaattaatc aattagtacc
    13921 tttagctgat aatagtgctt taaaaactgc taagacgaaa cttgatgaag aaatcaataa
    13981 atcagtaact actgatggta tgacacaatc atcaatccaa gcatatgaaa atgctaaacg
    14041 tgcgggtcaa acagaatcaa caaatgcaca aaatgttatt aacaatggtg atgcgactga
    14101 ccaacaaatt gccgcagaaa aaacaaaagt agaagaaaaa tataatagct taaaacaagc
    14161 aattgctgga ttaactccag acttggcacc attacaaact gcaaaaactc agttgcaaaa
    14221 tgatattgat cagccaacga gtacgactgg tatgacaagc gcatctattg cagcatttaa
    14281 tgaaaaactt tcagcagcta gaactaaaat tcaagaaatt gatcgtgtat tagcctcaca
    14341 tccagatgtt gcgacaatac gtcaaaacgt gacagcagcg aatgccgcta aatcagcact
    14401 tgatcaagca cgtaatggct taacagtcga taaagcgcct ttagaaaatg cgaaaaatca
    14461 actacaacat agtattgaca cgcaaacaag tacaactggt atgacacaag actctataaa
    14521 tgcatacaat gcgaagttaa cagctgcacg taataagatt caacaaatca atcaagtatt
    14581 agcaggttca ccgactgtag aacaaattaa tacaaatacg tctacagcaa atcaagctaa
    14641 atctgattta gatcatgcac gtcaagcttt aacaccagat aaagcgccgc ttcaaactgc
    14701 gaaaacgcaa ttagaacaaa gcattaatca accaacggat acaacaggta tgacgaccgc
    14761 ttcgttaaat gcgtacaacc aaaaattaca agcagcgcgt caaaagttaa ctgaaattaa
    14821 tcaagtgttg aatggcaacc caactgtcca aaatatcaat gataaagtga cagaggcaaa
    14881 ccaagctaag gatcaattaa atacagcacg tcaaggttta acattagata gacagccagc
    14941 gttaacaaca ttacatggtg catctaactt aaaccaagca caacaaaata atttcacgca
    15001 acaaattaat gctgctcaaa atcatgctgc gcttgaaaca attaagtcta acattacggc
    15061 tttaaatact gcgatgacga aattaaaaga cagtgttgcg gataataata caattaaatc
    15121 agatcaaaat tacactgacg caacaccagc taataaacaa gcgtatgata atgcagttaa
    15181 tgcggctaaa ggtgtcattg gagaaacgac taatccaacg atggatgtta acacagtgaa
    15241 ccaaaaagca gcatctgtta aatcgacgaa agatgcttta gatggtcaac aaaacttaca
    15301 acgtgcgaaa acagaagcaa caaatgcgat tacgcatgca agtgatttaa accaagcaca
    15361 aaagaatgca ttaacacaac aagtgaatag tgcacaaaac gtgcaagcag taaatgatat
    15421 taaacaaacg actcaaagct taaatactgc tatgacaggt ttaaaacgtg gcgttgctaa
    15481 tcataaccaa gtcgtacaaa gtgataatta tgtcaacgca gatactaata agaaaaatga
    15541 ttacaacaat gcatacaacc atgcgaatga cattattaat ggtaatgcac aacatccagt
    15601 tataacacca agtgatgtta acaatgcttt atcaaatgtc acaagtaaag aacatgcatt
    15661 gaatggtgaa gctaagttaa atgctgcgaa acaagaagcg aatactgcat taggtcattt
    15721 aaacaattta aataatgcac aacgtcaaaa cttacaatcg caaattaatg gtgcgcatca
    15781 aattgatgca gttaatacaa ttaagcaaaa tgcaacaaac ttgaatagtg caatgggtaa
    15841 cttaagacaa gctgttgcag ataaagatca agtgaaacgt acagaagatt atgcggatgc
    15901 agatacagct aaacaaaatg catataacag tgcagtttca agtgccgaaa caatcattaa
    15961 tcaaacaaca aatccaacga tgtctgttga tgatgttaat cgtgcaactt cagctgttac
    16021 ttctaataaa aatgcattaa atggttatga aaaattagca caatctaaaa cagatgctgc
    16081 aagagcaatt gatgcattac cacatttaaa taatgcacaa aaagcagatg ttaaatctaa
    16141 aattaatgct gcatcaaata ttgctggcgt aaatactgtt aaacaacaag gtacagattt
    16201 aaatacagcg atgggtaact tgcaaggtgc aatcaatgat gaacaaacga cgcttaatag
    16261 tcaaaactat caagatgcga cacctagtaa gaaaacagca tacacaaatg cggtacaagc
    16321 tgcgaaagat attttaaata aatcaaatgg tcaaaataaa acgaaagatc aagttactga
    16381 agcgatgaat caagtgaatt ctgctaaaaa taacttagat ggtacgcgtt tattagatca
    16441 agcgaagcaa acagcaaaac agcagttaaa taatatgacg catttaacaa ctgcacaaaa
    16501 aacgaattta acaaaccaaa ttaatagtgg tactactgtc gctggtgttc aaacggttca
    16561 atcaaatgcc aatacattag atcaagccat gaatacgtta agacaaagta ttgccaacaa
    16621 agatgcgact aaagcaagtg aagattacgt agatgctaat aatgataagc aaacagcata
    16681 taacaacgca gtagctgctg ctgaaacgat tattaatgct aatagtaatc cagaaatgaa
    16741 tccaagtacg attacacaaa aagcagagca agtgaatagt tctaaaacgg cacttaacgg
    16801 tgatgaaaac ttagctgctg caaaacaaaa tgcgaaaacg tacttaaaca cattgacaag
    16861 tattacagat gctcaaaaga acaatttgat tagtcaaatt actagtgcga caagagtgag
    16921 tggtgttgat actgtaaaac aaaatgcgca acatctagac caagctatgg ctagcttaca
    16981 gaatggtatt aacaacgaat ctcaagtgaa atcatctgag aaatatcgtg atgctgatac
    17041 aaataaacaa caagagtatg ataatgctat tactgcagcg aaagcgattt taaataaatc
    17101 gacaggtcca aacactgcgc aaaatgcagt tgaagcagca ttacaacgtg ttaataatgc
    17161 gaaagatgca ttgaatggtg atgcaaaatt aattgcagct caaaacgcag cgaaacaaca
    17221 tttaggtact ttaacgcata tcactacagc tcaacgtaat gatttaacaa atcaaatttc
    17281 acaagctaca aacttagctg gtgttgaatc tgttaaacaa aatgcgaata gtttagatgg
    17341 tgctatgggt aacttacaaa cggctatcaa cgataagtca ggaacattag cgagccaaaa
    17401 cttcttggat gctgatgagc aaaaacgtaa tgcatacaat caagctgtat cagcagccga
    17461 aaccatttta aataaacaaa ctggaccgaa tacagcgaaa acagcagtcg aacaagcact
    17521 taataatgtt aataatgcga aacatgcatt aaatggtacg caaaacttaa acaatgcgaa
    17581 acaagcagcg attacagcaa tcaatggcgc atctgattta aatcaaaaac aaaaagatgc
    17641 attaaaagca caagctaatg gtgctcaacg cgtatctaat gcacaagatg tacagcacaa
    17701 tgcgactgaa ctgaacacgg caatgggcac attaaaacat gccatcgcag ataagacgaa
    17761 tacgttagca agcagtaaat atgttaatgc cgatagcact aaacaaaatg cttacacaac
    17821 taaagttacc aatgctgaac atattattag cggtacgcca acggttgtta cgacaccttc
    17881 agaagtaaca gctgcagcta atcaagtaaa cagcgcgaaa caagaattaa atggtgacga
    17941 aagattacgt gaagcaaaac aaaacgccaa tactgctatt gatgcattaa cacaattaaa
    18001 tacacctcaa aaagctaaat taaaagaaca agtgggacaa gccaatagat tagaagacgt
    18061 acaaactgtt caaacaaatg gacaagcatt gaacaatgca atgaaaggct taagagatag
    18121 tattgctaac gaaacaacag tcaaaacaag tcaaaactat acagacgcaa gtccgaataa
    18181 ccaatcaaca tataatagcg ctgtgtcaaa tgcgaaaggt atcattaatc aaactaacaa
    18241 tccgactatg gatactagtg cgattaccca agctacaaca caagtgaata atgctaaaaa
    18301 tggtttaaac ggtgctgaaa acttaagaaa tgcacaaaac actgctaagc aaaacttaaa
    18361 tacattatca cacttaacaa ataaccaaaa atctgccatc tcatcacaaa ttgatcgtgc
    18421 aggtcatgtg agtgaggtaa ctgctactaa aaatgcagca actgagttga atacgcaaat
    18481 gggtaacttg gaacaagcta tccatgatca aaacacagtt aaacaaagtg ttaaatttac
    18541 tgatgcagat aaagctaaac gtgatgcgta tacaaatgcg gtaagcagag ctgaagcaat
    18601 tctgaataaa acgcaaggtg caaatacgtc taaacaagat gttgaagcgg ctattcaaaa
    18661 tgtttcaagt gctaaaaatg cattgaatgg tgatcaaaac gttacaaatg cgaagaatgc
    18721 agctaaaaat gcattaaata acttaacgtc aattaataat gcacaaaaac gtgacttaac
    18781 aactaaaatt gatcaagcaa caactgtagc tggtgttgaa gctgtatcta atacgagtac
    18841 acaattgaat acagcgatgg ctaacttgca aaatggtatt aatgataaaa caaatacact
    18901 agcaagtgaa aactatcatg atgctgattc agataagaaa actgcttata ctcaagccgt
    18961 tacgaacgca gaaaatattt taaataaaaa tagtggatca aatttagaca aaactgccgt
    19021 tgaaaacgcg ttgtcacaag ttgctaatgc gaaaggtgcc ctaaatggta accataattt
    19081 agagcaagct aaatcaaatg caaacactac tataaacgga cttcaacatt taacaactgc
    19141 tcaaaaagat aaattgaaac aacaagtgca acaagcacaa aatgttgcag gtgtagatac
    19201 tgttaaatca agtgccaaca cattaaatgg tgctatgggt acgttaagaa atagcataca
    19261 agataacaca gctacgaaaa atggccaaaa ctatcttgat gctacagaac gtaacaaaac
    19321 aaactataac aatgctgttg atagtgctaa tggtgtcatt aatgcaacaa gcaatccaaa
    19381 tatggatgct aatgcaatta accaaatcgc tacacaagtg acatcaacga aaaatgcatt
    19441 agatggtaca cataatttaa cgcaagcgaa acaaacagca acaaatgcca tcgatggtgc
    19501 tactaactta aataaagcgc aaaaagatgc gttaaaagca caagttacaa gtgcgcaacg
    19561 tgttgcaaat gtaacaagta tccaacaaac tgcaaatgaa cttaatacag ctatgggtca
    19621 attacaacat ggtattgatg atgaaaatgc aacaaaacaa actcaaaaat atcgtgacgc
    19681 tgaacaaagt aagaaaactg cttatgatca agctgtagct gctgcgaaag caattttaaa
    19741 taaacaaaca ggttcaaatt cagataaagc agcagttgac cgtgcattac aacaagtaac
    19801 aagtacgaaa gatgcattga atggtgatgc aaaactggca gaagcgaaag cggcagctaa
    19861 acaaaactta ggcactttaa accatattac gaatgcacaa cgtactgact tagaaggcca
    19921 aatcaatcaa gcgacgactg ttgatggcgt taatactgta aaaacaaatg ccaatacatt
    19981 agacggcgca atgaatagct tacaaggttc aatcaatgat aaagatgcga cattaagaaa
    20041 tcaaaattat cttgatgcgg atgaatcaaa acgaaatgca tatacgcaag ctgtcacagc
    20101 ggctgaaggc attttaaata aacaaactgg tggtaacaca tctaaagcag acgttgataa
    20161 tgcattaaat gcagttacaa gagcgaaagc ggctttaaat ggtgctgaca acttaagaaa
    20221 tgcgaaaact tcagcaacaa atacgattga tggtttacct aacttaacac aattacaaaa
    20281 agacaacttg aagcatcaag ttgaacaagc gcaaaatgta gcaggtgtaa atggtgttaa
    20341 agataaaggt aatacgttaa atactgccat gggtgcatta cgtacaagta tccaaaatga
    20401 taatacgacg aaaacaagtc aaaattatct tgatgcatct gacagcaaca aaaataatta
    20461 caatactgct gtaaataatg caaatggtgt tattaatgca acgaacaatc caaatatgga
    20521 tgctaatgcg attaatggca tggcaaatca agtcaataca acaaaagcag cgttaaatgg
    20581 tgcacaaaac ttagctcaag ctaaaacaaa tgcgacgaac acaattaaca acgcacatga
    20641 cttaaaccaa aaacaaaaag atgcattaaa aacacaagtt aacaatgcac aacgtgtatc
    20701 tgatgcaaat aacgttcaac acactgcaac tgaattgaac agtgcgatga cagcacttaa
    20761 agcagctatt gctgataaag aaagaacaaa agcaagcggt aattatgtca atgctgatca
    20821 agaaaaacgt caagcgtatg attcaaaagt gactaacgct gaaaatatca ttagtggtac
    20881 accgaatgcg acattaacag tcaatgacgt aaatagtgcg gcatcacaag tcaatgcggc
    20941 taaaacagca ttaaatggtg ataacaactt acgtgtagcg aaagagcatg ccaacaatac
    21001 aattgacggc ttagcacaat tgaataatgc acaaaaagca aaattaaaag aacaagttca
    21061 aagtgcaact acattagatg gtgttcaaac tgttaaaaat agttctcaaa cgttgaatac
    21121 agcgatgaaa ggcttaagag atagtattgc gaatgaagca acaattaaag caggtcaaaa
    21181 ctacactgac gcaagtccaa ataatcgtaa cgagtacgac agtgcagtta ctgcagcaaa
    21241 agcaatcatt aatcaaacat cgaacccaac gatggaacca aatactatta cgcaagtaac
    21301 atcacaagtg acaactaaag aacaggcatt aaatggtgcg cgaaacttag ctcaagctaa
    21361 gacaactgcg aaaaacaact tgaataactt aacatcaatt aacaatgcac aaaaagatgc
    21421 gttaacgcgt agcattgatg gtgcaacaac agtagctggt gtaaatcaag aaactgcaaa
    21481 agcaacagaa ttaaataacg caatgcatag tttacaaaat ggtatcaatg atgagacaca
    21541 aacaaaacaa actcagaaat acctagatgc agagccaagt aagaaatcag cttatgatca
    21601 agcagtaaat gcagcgaaag caattttaac aaaagctagt ggtcaaaatg tagacaaagc
    21661 agcagttgaa caagcattgc aaaatgtgaa cagtacgaag acggcgttga acggtgatgc
    21721 gaaattaaat gaagctaaag cagctgcgaa acaaacgtta ggtacattaa cacacattaa
    21781 taatgcacaa cgtacagcgt tagacaatga aattacacaa gcaacaaatg ttgaaggtgt
    21841 taatacagtt aaagccaaag cgcaacaatt agatggtgct atgggtcaat tagaaacatc
    21901 aattcgtgat aaagacacga cgttacaaag tcaaaattat caagatgctg atgatgctaa
    21961 acgaactgct tattctcaag cagtaaatgc agcagcaact attttaaata aaacagctgg
    22021 cggtaataca cctaaagcag atgttgaaag agcaatgcaa gctgttacac aagcaaatac
    22081 tgcattaaac ggtattcaaa acttagatcg tgcgaaacag gctgctaaca cagcgattac
    22141 aaatgcttcg gacttaaata caaaacaaaa agaagcatta aaagcacaag taacaagtgc
    22201 aggacgtgta tctgcagcaa atggtgttga acatactgcg actgaattaa atactgcgat
    22261 gacagcttta aagcgtgcca ttgctgataa agctgagaca aaagctagtg gtaactatgt
    22321 caatgctgat gcgaataaac gtcaagcata tgatgaaaaa gttacagctg ccgaaaatat
    22381 cgttagtggt acaccaacac caacgttaac accagcagat gttacaaatg cagcaacgca
    22441 agtaacgaat gctaagacgc agttaaacgg taatcataat ttagaagtag cgaaacaaaa
    22501 tgctaacact gcaattgatg gtttaacttc tttaaatggt ccgcaaaaag caaaacttaa
    22561 agaacaagtg ggtcaagcga cgacgttgcc aaatgttcaa actgttcgtg ataatgcaca
    22621 aacattaaac actgcaatga aaggtctacg agatagcatt gcgaatgaag caacgattaa
    22681 agcaggtcaa aactacacag atgcaagtca aaacaaacaa actgactaca acagtgcagt
    22741 cactgcagca aaagcaatca ttggtcaaac aactagtcca tcaatgaatg cgcaagaaat
    22801 taatcaagcg aaagaccaag tgacagctaa acaacaagcg ttaaacggtc aagaaaactt
    22861 aagaactgcg caaacaaatg cgaagcaaca tttgaacggc ttaagtgact taactgacgc
    22921 tcaaaaagat gcagtgaaac gtcaaatcga aggtgcaacg catgttaatg aagtaacaca
    22981 agcacaaaat aatgcggatg cattaaatac agctatgacg aacttgaaaa atggtattca
    23041 agatcagaat acgattaagc aaggtgttaa cttcactgat gccgacgaag cgaaacgtaa
    23101 tgcatataca aatgcagtga cgcaagctga acaaatttta aataaagcac aaggtccaaa
    23161 tacttcaaaa gacggtgtcg aaactgcgtt agaaaatgta caacgtgcta aaaacgaatt
    23221 gaacggtaat caaaatgttg cgaacgctaa gacaactgcg aaaaatgcat tgaataacct
    23281 aacatcaatt aataatgcac aaaaagaagc attgaaatca caaattgaag gtgcgacaac
    23341 agttgcaggt gtaaatcaag tgtctacaac ggcatctgaa ttaaatacag caatgagcaa
    23401 cttacaaaat ggtattaatg atgaagcagc tacaaaagca gctcagaaat atactgatgc
    23461 agatagagaa aaacaaactg catacaatga cgctgtaaca gcagctaaaa cgttattaga
    23521 taaaacagct ggttcaaatg acaataaagc agctgttgaa caagcattac aacgtgtgaa
    23581 tactgctaaa acagcattaa atggtgacga gcgattaaat gaagcgaaga acacagctaa
    23641 acaacaagta gcgacaatgt cacacttaac tgatgctcaa aaagcaaact taacatcgca
    23701 aatcgaaagt ggtacgactg ttgcaggtgt tcaaggtatt caagctaatg ccggtacttt
    23761 agatcaagca atgaatcaat taagacaaag tattgcttct aaagatgcga ctaaatcaag
    23821 cgaagattat caagacgcga atgcagattt acaaaatgca tacaatgatg cggtaactaa
    23881 tgctgaaggt attattagtg caacgaataa ccctgaaatg aatcctgata caattaacca
    23941 aaaagcgagc caagtgaaca gtgcgaagtc tgcattgaac ggtgatgaaa aattagcagc
    24001 agcaaaacaa actgcgaaat cagatatcgg tcgtttgaca gacttgaaca atgcacaacg
    24061 aactgcggca aatgctgaag tggatcaagc accaaatctt gcagctgtca cagcggctaa
    24121 aaataaagca acatcgttaa acacagcgat gggtaatttg aaacatgcac ttgctgaaaa
    24181 ggataatacg aaacgtagtg tcaattacac agatgcggat caaccaaaac aacaagcgta
    24241 tgatactgcg gttacacaag cagaagcaat tactaatgct aatggcagca acgcgaatga
    24301 aacacaagtt caagcagcac taaaccaatt gaatcaagct aaaaatgact tgaatggtga
    24361 taataaagtt gctcaagcaa aagagtcagc gaaacgtgca ttagcttcat atagtaactt
    24421 gaataatgcg caatcaactg cagcaattag tcaaattgac aatgcaacga cagtagcagg
    24481 cgtaactgct gcacaaaata ctgctaatga attaaataca gcaatgggtc aacttcaaaa
    24541 tggtataaat gaccaaaaca ctgttaaaca acaagtgaac tttacagatg ctgaccaagg
    24601 taagaaagat gcttacacaa atgctgttac gaatgctcaa ggtattttag ataaagcaca
    24661 cggtcaaaat atgacgaaag cacaagttga agctgcatta aatcaagtaa cgactgctaa
    24721 gaatgcttta aacggtgacg caaatgtaag acaagcaaaa tcagatgcga aagcaaactt
    24781 aggtacatta acacacttaa ataatgcaca aaaacaagat ttaacatcac aaatcgaagg
    24841 cgcaacaaca gtcaacggtg taaatggtgt taaaacgaaa gcacaagact tagatggtgc
    24901 aatgcaacga ttacaatcag ctatagcaaa taaagatcaa actaaagcga gcgaaaatta
    24961 catcgacgca gatccaacta agaaaacagc atttgataat gctatcacac aagctgaatc
    25021 ttacttaaat aaagatcacg gtgcgaataa agataagcaa gctgttgaac aagcaattca
    25081 aagtgtaacg tctactgaaa atgctttgaa cggtgacgcg aacttacaac gcgctaaaac
    25141 tgaagctata caagctatcg ataacttgac acatttgaat acaccacaaa aaacagcatt
    25201 aaaacaacaa gtgaacgctg cgcaacgtgt atcaggtgta actgatctga aaaatagtgc
    25261 tacatcactt aataatgcga tggatcaatt aaaacaagca attgctgatc atgacacaat
    25321 tgtagctagt ggtaattaca ctaacgcgag tcctgataag caaggtgctt atactgatgc
    25381 atataatgct gcgaaaaaca ttgtaaatgg ttcgcctaat gtgattacaa atgcagcaga
    25441 tgttacagca gcaacacaac gtgttaataa tgctgaaaca ggtttaaacg gtgatacaaa
    25501 cttagcaact gcgaagcaac aagctaaaga tgcattacgt caaatgacac atttatctga
    25561 tgcacaaaaa caaagtatta ctggtcaaat tgatagcgcg acacaagtaa ctggcgttca
    25621 aagtgtgaaa gacaacgcga caaatcttga taatgcaatg aatcaacttc gaaatagtat
    25681 tgcgaataaa gatgatgtaa aagcgagtca accatatgtt gatgcagata gagataaaca
    25741 aaatgcatac aatacagcag ttacaaatgc tgaaaatatc attaatgcaa cgagtcagcc
    25801 gacacttgat ccatctgcag taacacaagc agctaatcaa gtgagcacta acaaaactgc
    25861 gcttaatggt gcacaaaact tagcgaataa aaagcaagaa acgactgcta acatcaacca
    25921 attaagtcat ttaaataatg ctcaaaagca agatttaaat acgcaagtga caaatgcacc
    25981 aaatattagc acagtaaatc aagtgaaaac taaagctgaa caattagatc aagcaatgga
    26041 acgtttaatc aacggaatcc aagacaaaga tcaagtgaaa caaagtgtta actttacaga
    26101 tgcagatcca gaaaaacaaa cagcatacaa caatgcggta actgctgctg aagtaacgca
    26161 agccattcaa accgctcagg aactgaatac agcgatgggt aacttgaaaa atagcttgaa
    26221 tgataaagac actacacttg gcagtcaaaa ctttgcagat gcagatccag agaagaaaaa
    26281 tgcatacaat gaagcggttc ataatgctga aaatatttta aataaatcta caggtacgaa
    26341 cgtgcctaaa gatcaagttg aagcagctat gaatcaagtg aatgctacaa aagcagcgct
    26401 taatggtact caaaaccttg aaaaagctaa acaacacgca aatacagcaa ttgacggttt
    26461 aagccattta acaaatgcac aaaaagaggc attaaaacaa ttggtacaac aatcgactac
    26521 tgttgcagaa gcacaaggta atgagcaaaa agcaaacaat gttgatgcag caatggacaa
    26581 attacgtcaa agtattgcag ataatgcgac aacaaaacaa aaccaaaatt atactgatgc
    26641 aagtcagaat aaaaaggatg cgtacaataa tgctgtcaca actgcacaag gtattattga
    26701 tcaaactaca agtccaactt tagatccgac tgttatcaat caagctgctg gacaagtaag
    26761 cacaactaaa aatgcattaa atggtaatga aaacctagag gcagcgaaac aacaagcgtc
    26821 acaatcatta ggttcattag ataacttaaa taatgcgcaa aaacaaacag ttactgatca
    26881 aattaatggc gcgcatactg ttgatgaagc aaatcaaatt aagcaaaatg cgcaaaactt
    26941 aaatacagcg atgggtaact tgaaacaagc gatagctgac aaagatgcta cgaaagcgac
    27001 agttaacttc actgatgcag atcaagcaaa acaacaagca tataacactg ctgttacaaa
    27061 tgctgaaaat atcatttcaa aagctaatgg cggcaatgca acacaagctg aagttgaaca
    27121 agcaatcaaa caagttaatg ctgcaaaaca agcattaaat ggtaatgcca acgttcaaca
    27181 tgcaaaagac gaagcaacag cattaattaa tagctctaat gaccttaacc aagcacaaaa
    27241 agacgcatta aaacaacaag ttcaaaatgc aactactgta gctggtgtaa acaatgttaa
    27301 acaaacagca caagagttaa acaatgctat gacacaatta aaacaaggca ttgcagataa
    27361 agaacaaaca aaagctgatg gtaactttgt caatgcagat cctgataagc aaaatgcata
    27421 taatcaagca gtagcgaaag ctgaagcatt aattagtgct acgcctgatg ttgtcgttac
    27481 acctagcgaa attactgcag cgttaaataa agttacgcaa gctaaaaatg atttaaatgg
    27541 taatacaaac ttagcaacgg cgaaacaaaa tgttcaacat gctattgatc aattgccaaa
    27601 cttaaaccaa gcgcaacgtg atgaatacag caaacaaatc acgcaagcaa cacttgtacc
    27661 aaacgtcaat gctattcaac aagcggcgac aacgcttaat gacgcgatga cacaattgaa
    27721 acaaggtatt gcgaataaag cacaaattaa aggtagcgag aactatcacg atgctgatac
    27781 tgacaagcaa acagcatatg ataatgcagt aacaaaagca gaagaattgt taaaacaaac
    27841 aacaaatcca acaatggatc caaatacaat tcaacaagca ttaactaaag tgaatgacac
    27901 aaatcaagca cttaacggta atcaaaaatt agctgatgcc aaacaagatg ctaagacaac
    27961 acttggtaca ctagatcatt taaatgatgc tcaaaaacaa gcgctaacaa ctcaagttga
    28021 acaagcacca gatattgcaa cagttaataa tgttaagcaa aatgctcaaa atctgaataa
    28081 tgctatgact aacttaaaca atgcattaca agataaaact gagacattaa atagcattaa
    28141 ctttactgat gcagatcaag ctaagaaaga tgcttatact aatgcggttt cacatgcaga
    28201 aggtatttta tctaaagcaa atggcagcaa tgcaagtcaa actgaagtgg aacaagcgat
    28261 gcaacgtgtg aacgaagcga aacaagcatt gaatggtaat gacaatgtac aacgtgcaaa
    28321 agatgcagcg aaacaagtga ttacaaatgc aaatgattta aatcaagcgc aaaaagatgc
    28381 attaaaacaa caagtcgatg ctgcgcaaac tgttgcaaat gtaaacacga ttaagcaaac
    28441 agcacaagat ttaaatcaag caatgacaca attgaaacaa ggtattgcag ataaagacca
    28501 aactaaagca aatggtaact ttgtcaatgc tgatactgat aagcaaaatg cttacaacaa
    28561 tgcggtagca catgctgaac aaataattag tggtacacca aatgcaaacg tggatccaca
    28621 acaagtggct caagcgttac aacaagtgaa tcaagctaag ggtgatttaa acggtaacca
    28681 taacttacaa gttgctaaag acaatgcaaa tacagccatt gatcagttac caaacttaaa
    28741 tcaaccacaa aaaacagcat taaaagacca agtgtcgcat gcagaacttg ttacaggtgt
    28801 taatgctatt aagcaaaatg ctgatgcgtt aaataatgca atgggtacat tgaaacaaca
    28861 aattcaagcg aacagtcaag taccacagtc agttgacttt acacaagcgg atcaagacaa
    28921 acaacaagca tataacaatg cggctaacca agcgcaacaa atcgcaaatg gcataccaac
    28981 acctgtattg acgcctgata cagtaacaca agcagtgaca actatgaatc aagcgaaaga
    29041 tgcattaaac ggtgatgaaa aattagcaca agcgaaacaa gaagctttag caaatcttga
    29101 tacgttacgc gatttaaatc aaccacaacg tgatgcatta cgtaaccaaa tcaatcaagc
    29161 acaagcgtta gctacagttg aacaaactaa acaaaatgca caaaatgtga atacagcaat
    29221 gagtaacttg aaacaaggta ttgcaaacaa agatactgtc aaagcaagtg agaactatca
    29281 tgatgctgat gccgataagc aaacagcata tacaaatgca gtgtctcaag cggaaggtat
    29341 tatcaatcaa acgacaaatc caacgcttaa cccagatgaa ataacacgtg cattaactca
    29401 agtgactgat gctaaaaatg gcttaaacgg tgaagctaaa ttggcaactg aaaagcaaaa
    29461 tgctaaagat gccgtaagtg ggatgacgca tttaaacgat gctcaaaaac aagcattaaa
    29521 aggtcaaatc gatcaatcgc ctgaaattgc tacagtgaac caagttaaac aaacagcaac
    29581 gagcctagat caagcaatgg atcaattatc acaagctatt aatgataaag ctcaaacatt
    29641 agcggacggt aattacttaa atgcagatcc tgacaaacaa aatgcgtata aacaggcagt
    29701 agcaaaagct gaagcattat tgaataaaca aagtggtact aatgaagtac aagcacaagt
    29761 tgaaagcatc actaatgaag tgaacgcagc gaaacaagca ttaaatggta atgacaattt
    29821 ggcaaatgca aaacaacaag caaaacaaca attggcgaac ttaacacact taaatgatgc
    29881 acaaaaacaa tcatttgaaa gtcaaattac acaagcgcca cttgttacag atgtcactac
    29941 gattaatcaa aaagcacaaa cgttagatca tgcgatggaa ttattaagaa atagtgttgc
    30001 ggataatcaa acgacattag cgtctgaaga ttatcatgat gcaactgcgc aaagacaaaa
    30061 tgactataac caagctgtaa cagctgctaa taatatcatt aatcaaacta catcgcctac
    30121 gatgaatcca gatgatgtta atggtgcaac gacacaagtg aataatacga aagttgcatt
    30181 agatggtgat gaaaaccttg cagcagctaa acaacaagca aacaacagac ttgatcaatt
    30241 agatcatttg aataatgcgc aaaagcaaca gttacaatca caaattacgc aatcatctga
    30301 tattgctgca gttaatggtc acaaacaaac agcagaatct ttaaatactg cgatgggtaa
    30361 cttaattaat gcgattgcag atcatcaagc cgttgaacaa cgtggtaact tcatcaatgc
    30421 tgatactgat aaacaaactg cttataatac agcggtaaat gaagcagcag caatgattaa
    30481 caaacaaact ggtcaaaatg cgaaccaaac agaagtagaa caagctatca ctaaagttca
    30541 aacaacactt caagcgttaa atggagacca taatttacaa gttgctaaaa caaatgcgac
    30601 gcaagcaatt gatgctttaa caagcttaaa tgatcctcaa aaaacagcat taaaagacca
    30661 agttacagct gcaactttag taactgcagt tcatcaaatt gaacaaaatg cgaatacgct
    30721 taaccaagca atgcatggtt taagacagag cattcaagat aacgcagcaa ctaaagcaaa
    30781 tagcaaatat atcaacgaag atcaaccaga gcaacaaaac tatgatcaag ctgttcaagc
    30841 cgcaaataat attatcaatg aacaaactgc aacattagat aataatgcga ttaatcaagc
    30901 agcgacaact gtgaatacaa cgaaagcagc attacatggt gatgtgaagt tacaaaatga
    30961 taaagatcat gctaagcaaa cggttagtca attagcacat ctaaacaatg cacaaaaaca
    31021 tatggaagat acgttaattg atagtgaaac aactagaaca gcagttaagc aagatttgac
    31081 tgaagcacaa gcattagatc aacttatgga tgcattacaa caaagtattg ctgacaaaga
    31141 tgcaacacgt gcgagcagtg catatgtcaa tgcagaaccg aataaaaaac aatcctatga
    31201 tgaagcagtt caaaatgctg agtctatcat tgcaggatta aataatccaa ctatcaataa
    31261 aggtaatgta tcaagtgcga ctcaagcagt aatatcatct aaaaatgcat tagatggtgt
    31321 tgaacgatta gctcaagata agcaaactgc tggaaattct ctaaatcatt tagatcaatt
    31381 aacaccagct caacaacaag cgctagaaaa tcaaattaat aatgcaacaa ctcgtggcga
    31441 agtagcacaa aaattaactg aagcacaagc acttaaccaa gcaatggaag ctttacgtaa
    31501 tagcattcaa gatcaacagc aaacggaagc gggtagcaag tttatcaatg aagataaacc
    31561 acaaaaagat gcttaccaag cagcagttca aaatgcaaaa gatttaatta atcaaactaa
    31621 caatccaacg cttgataaag cacaagttga acaattgaca caagctgtta accaagctaa
    31681 agataaccta cacggtgatc aaaaacttgc agacgataaa caacatgcgg ttactgattt
    31741 aaatcaatta aatggtttga ataatccgca acgtcaagca cttgaaagcc aaataaacaa
    31801 cgcagcaact cgtggcgaag tagcacaaaa attagctgaa gcaaaagcgc ttgatcaagc
    31861 aatgcaagca ttacgtaata gtattcaaga tcaacaacaa acagaatctg gtagcaagtt
    31921 tatcaatgaa gataaaccgc aaaaagatgc ttaccaagca gcagttcaaa atgcaaaaga
    31981 tttaattaac caaacaggta atccaacact cgacaaatca caagtagaac aattgacaca
    32041 agcagtaaca actgcaaaag ataatctaca tggtgatcaa aaacttgctc gtgatcaaca
    32101 acaagcagta acaactgtaa atgcattgcc aaacttaaat catgcacaac aacaagcatt
    32161 aactgatgct ataaatgcag cgcctacaag aacagaggtt gcacaacatg ttcaaactgc
    32221 tactgaactt gatcacgcga tggaaacatt gaaaaataaa gttgatcaag tgaatacaga
    32281 taaggctcaa ccaaattaca ctgaagcgtc aactgataaa aaagaagcag tagatcaagc
    32341 gttacaagct gcagaaagca ttacagatcc aactaatgct tcaaatgcga ataaagacgc
    32401 tgtagaccaa gtattaacta agcttcaaga aaaagaaaat gagttaaatg gtaatgagag
    32461 agtcgctgaa gctaaaacac aagcgaaaca aactattgac caattaacac atttaaatgc
    32521 tgatcaaatt gcaactgcta aacaaaacat tgatcaagcg acgaaacttc aaccaattgc
    32581 tgaattagta gatcaagcaa cgcaattgaa tcaatctatg gatcaattac aacaagcagt
    32641 taatgaacat gctaacgttg agcaaactgt agattacaca caagcagatt cagataaaca
    32701 aaatgcttat aaacaagcta ttgctgatgc tgaaaatgta ttgaaacaaa atgcgaataa
    32761 gcaacaagtg gatcaagcac ttcaaaatat tttaaatgca aaacaagcat taaatggtga
    32821 tgaacgtgta gcacttgcta aaacaaatgg taaacatgac atcgaccaat tgaatgcatt
    32881 aaacaatgct caacaagatg gatttaaagg tcgcatcgat caatcaaacg atttaaatca
    32941 aatccaacaa attgtagatg aggctaaggc acttaatcgt gcaatggatc aattgtcaca
    33001 agaaatcact gacaatgaag gacgcacgaa aggtagcacg aactatgtca atgcagatac
    33061 acaagtcaaa caagtatatg atgaaacggt tgataaagcg aaacaagcac ttgataaatc
    33121 gactggtcaa aacttaactg caaaacaagt tatcaaatta aatgatgcag tcactgcagc
    33181 taagaaagca ttaaatggtg aagaaagact taataatcgt aaagctgaag cattacaaag
    33241 attggatcaa ttaacacatc taaacaatgc tcaaagacaa ttagcaatcc aacaaattaa
    33301 taatgctgaa acgctaaata aagcatctcg agcaattaat agagcaacta aattagataa
    33361 tgcaatgggt gcagtacaac aatatattga cgaacagcac cttggtgtta tcagcagcac
    33421 aaattacatc aatgcagatg acaatttgaa agcaaattat gataatgcaa ttgcgaatgc
    33481 agcacatgag ttagataaag tgcaaggtaa tgcaattgca aaagctgaag cagagcaatt
    33541 gaaacaaaat attatcgatg ctcaaaatgc attaaatgga gaccaaaacc ttgcaaatgc
    33601 caaagataaa gcaaatgcgt ttgttaattc gttaaatgga ttaaatcaac agcaacaaga
    33661 tcttgcacat aaagcaatta acaatgccga tactgtatca gatgtaacag atattgttaa
    33721 taatcaaatt gacttaaatg atgcaatgga aacattgaaa catttagttg acaatgaaat
    33781 tccaaatgca gagcaaactg tcaattacca aaacgctgac gataatgcta aaacaaactt
    33841 cgatgatgcc aaacgtctag caaatacatt gctaaatagt gataacacaa atgtgaatga
    33901 tatcaatggc gcaatccaag cagtcaatga tgcaatccat aatcttaatg gtgatcaacg
    33961 actacaagat gctaaagaca aggcaattca atctattaat caagctttag ctaataagct
    34021 aaaagaaatc gaagcttcaa atgcgacgga tcaagacaag cttattgcga aaaataaagc
    34081 agaagaattg gcaaacagca tcatcaacaa cattaataaa gcaacaagta atcaggctgt
    34141 atctcaagtt caaacagcag gcaaccacgc gattgaacaa gtgcatgcca atgaaatacc
    34201 aaaagcaaaa attgatgcca ataaagacgt tgataagcaa gttcaagcat taattgacga
    34261 aattgatcga aatccaaatc taacagataa ggaaaaacaa gcacttaaag atcgtattaa
    34321 tcaaatactt caacaaggtc ataacggcat taacaatgcg atgactaaag aagaaattga
    34381 acaagccaaa gcacaacttg cgcaagcatt acaagacatc aaagatttag tgaaagctaa
    34441 agaagatgcg aaacaagatg ttgataaaca agttcaagct ttaattgacg aaatcgatca
    34501 aaatccaaat ctaacagata aggaaaaaca agcacttaaa tatcgaatta atcaaatact
    34561 tcaacaaggt cataacgaca ttaacaatgc gctgactaaa gaagaaattg agcaggcaaa
    34621 agcacaactt gcacaagcat tgcaagacat caaagattta gtgaaagcta aagaagatgc
    34681 gaaaaatgca ataaaagcct tagctaatgc gaagcgtgat caaatcaatt caaatccaga
    34741 tttaacacct gagcaaaaag caaaagcgct caaagaaatt gacgaagctg aaaaacgagc
    34801 actacaaaac gttgagaatg ctcaaactat agatcaatta aatcgaggat taaacttagg
    34861 tttagatgac attagaaata cacatgtatg ggaggttgat gaacaacctg ctgtaaatga
    34921 aatttttgaa gcaacacctg agcaaatcct agttaatggt gaactcattg tacatcgtga
    34981 tgacatcatt acagaacaag atattcttgc acacataaac ttaattgatc agctttcagc
    35041 agaagtcatc gatacaccat caactgcaac gatttctgat agcttaacag caaaagttga
    35101 agttacattg cttgatggat caaaagtgat tgttaatgtt cctgtaaaag ttgtagaaaa
    35161 agaattgtca gtagtcaaac aacaggcaat tgaatcaatc gaaaatgcgg cacaacaaaa
    35221 gattaatgaa atcaataata gtgtgacatt aacactggaa caaaaagaag ctgcaattgc
    35281 agaagttaat aagcttaaac aacaagcaat tgatcatgtt aacaatgcac ctgatgttca
    35341 ttcagttgaa gaaattcaac aacaagaaca agcgcatatt gaacaattta atccagaaca
    35401 atttacgatt gaacaagcaa aatcaaatgc aattaaatcg attgaagatg caattcaaca
    35461 tatgattgat gaaatcaaag ctcgtactga tctaacagat aaagagaagc aagaagctat
    35521 tgctaagtta aatcaattaa aagaacaagc aattcaagcg attcaacgtg cgcaaagcat
    35581 cgatgaaata agtgagcaat tggaacaatt taaagctcaa atgaaagcag ctaatccaac
    35641 agcaaaagaa ctagctaaac gcaagcaaga agctattagt agaattaaag acttttcaaa
    35701 tgaaaaaata aatagtattc gaaatagtga aattggcaca gctgatgaaa aacaagcagc
    35761 aatgaatcaa attaacgaaa ttgtgcttga aacaattaga gatattaata atgcgcatac
    35821 attacagcaa gttgaggctg cattgaacaa tggtattgct cgaatttcag cagtacaaat
    35881 tgtaacatct gatcgtgcta aacaatcgtc aagtactgga aatgaatcta atagccattt
    35941 aacaattggt tatggaactg caaatcatcc atttaacagt tcgactattg gacataaaaa
    36001 gaaacttgat gaagatgatg acattgatcc acttcatatg cgtcacttta gtaataattt
    36061 cggtaatgtt attaaaaacg ctattggtgt ggtgggtatc tctggtttac tagctagttt
    36121 ctggttcttc attgccaaac gtcgtcgtaa agaagatgaa gaggaagaat tagaaataag
    36181 agataataat aaagattcaa taaaagagac tttagacgat acaaaacatt taccactttt
    36241 atttgcgaaa cgtcgcagaa aagaagatga agaagatgtg gaagttacaa atgaaaacac
    36301 agatgaaaaa gtgttgaaag ataacgaaca ttcaccactc ttattcgcaa aacgacgcaa
    36361 agataaagag gaagatgttg aaacaacaac tagtattgaa tctaaagatg aggacgttcc
    36421 tttattattg gctaaaaaga aaaatcaaaa agataaccaa tccaaagaca aaaagtcagc
    36481 atcaaaaaat acttctaaaa aggtagcagc taaaaagaag aaaaagaaag ctaagaaaaa
    36541 taaaaaataa tttgtttctt tgataaatag aggagcaccg attgacatca catcagtcgg
    36601 tgctcctttt atttattctt tttaattaat ttatacaatg cctgttgagc gtgttgattc
    36661 gcttctttgt tttgttctct cggtatccat ttaacaaata ataaatcaaa atctttttca
    36721 aatatttcta tttgatcaaa ataaggtttg aaatttgcgt ttttcacata accagcttca
    36781 atgctatctg caattagctt tgagtctgta tataatagtg cgttttgaac atttaattca
    36841 cgtgcatgtt ctagtgcata aatacatgca gcccattctg cagtgtggtt atccatttcg
    36901 cctaactcat gtgtatatgt ataatgctgc tcatcttctt tgattacaat ggcacatgta
    36961 cttatgcctg gatttccttt cgtcgcagca tcaaaattta ttttcgccat aataaaccta
    37021 ctttctattc aatacttagt taaagttact attactgtaa tacaaaatat gttgggtaat
    37081 ccattaaaaa acacgcatca cttaaataag taacacgtgt ttaaaatact cgctgattca
    37141 aagatgattt tctaatacgt agtactgtaa tatacttcct aaaaaaatca tcttcaggct
    37201 gggacataaa tcaatgttct atgctctacg atgttatatt ggcagtagtt gactgaacga
    37261 aaatgcgctt gtaacaagct tttttcaatt ctagtcaggg gccccaacac agagaatttc
    37321 gaaaagaaat tctacaggca atgcgagttg gggtgtgggt cccaacacag aagatgacga
    37381 aaagtcagct tacaataatg tgcaagtttg ggatgggccc caacaaagag aaattggatt
    37441 cccaatttct acagacaatg caagttgggg tgggacgacg aaataaattt tgcgaaaata
    37501 ttatttctgt cccactccct taaaacttat tcttttgtgt agtaagtgcg ttaatagcct
    37561 tgatctaact tatcaatctt acctttacga taaaatgatt tagcaatata tccaaatggt
    37621 acattgaaaa ctgttgaagc taattttaat acgtacgttg taataaatat ttcaaataca
    37681 actgtaccag gtaaacttcc gataaatgcg atagctacaa ataaagctgt atcaattatt
    37741 gagcttaaaa atgtactacc atatgcacgg atgaaaaacg ttttatcaga actaaatact
    37801 tttttaatta gtgaaaagat aaatacatca atatgttgac caataatata tgcgacgatt
    37861 gagcctaaag caatgcgtgg cacaacatca aagattgcgt gtaatgcttt ttgtgccata
    37921 tcttctggtg caggaataaa atgtaatgac aattgcataa caataatcat aattaatgtt
    37981 gatgaaaagc ctaaccaaac tgctcttttt gcaactctac gcccataaat atcgtttaat
    38041 atatctgttg ctaaataaat agaagcaaac atgacattac ctaaagttgc tgaaatacca
    38101 aagatttcta cagttttaat cacttgtatg ttggcaatga ttgtgccaat tgcaacccat
    38161 gcaattaaac cttgtttacc aaaaaagcga tacataagta ccataagcac gaacgttgca
    38221 ataaacgtaa ctagtcctaa aatttcatta tacatattaa aatgtcctcc taaattttga
    38281 tcatgcgggt gtttagaaac cgctcaataa ataactaact cattttactg aaaaatcgtt
    38341 tgcgatgcaa tagcatttga atataaatac attttacgta tagaattgta aaaggtttca
    38401 ttcaaatctt agggtcaaaa atgttataat atttttatgt caaatttaaa acagtaacac
    38461 ttatttacaa ggttgcaata ttttgaagta ataaaggaag tgtcgcgtat tttaactttt
    38521 tcagagcaaa atgcactcgc gaaaatagat gatttaatga atacttattg caatcaatgt
    38581 ccaatcaaaa ctcgtctgcg taaattagag gggaaaacga aggcgcatca tttttgtatc
    38641 aatgagtgtt caatagggaa agaaataaaa caattaggaa atgaacttca ataggaggaa
    38701 gtcaaatgaa aattatatct atatcagaaa caccgaacca caacacaatg aagattacac
    38761 ttagtgaaag cagagaaggt atgacatcag atacgtatac taaagttgat gattcacagc
    38821 cagcatttat taatgacatc ttaaaggttg aaggcgttaa atcaattttc catgttatgg
    38881 actttatttc agtagataaa gaaaatgacg caaattggga aacagtattg ccaaaagtag
    38941 aggctgtatt cgaataaatt tttcatcaac tagtattcgg ggggaataaa gtatatggaa
    39001 attttacgta tagagccaac accaagtcca aatacaatga aagttgtttt gtcatataca
    39061 agagaagaca agttatctaa tacttataaa aaagtagaag aaacacaacc aagatttata
    39121 aatcagttgt tatctataga tggtatcact tccatttttc atgtcatgaa cttcttagct
    39181 gttgataagg caccaaaagc tgattgggaa gtcatattac ctgatattaa agctgctttt
    39241 tctgatgcga ataaggtttt agaatctgta aatgaacctc aaattgacaa tcattttggt
    39301 gaaattaaag ctgaattatt aacttttaag ggtataccgt atcaaattaa gctaacttct
    39361 gctgaccaag aattaagaga acaattacca caaacatatg ttgaccatat gactcaagcg
    39421 caaacagcac atgacaatat tgtttttatg cgtaaatggc tagatttagg aaatcgctat
    39481 ggaaatattc aagaagtaat ggatggtgtc ctagaagaag tgctaactac ctatccagaa
    39541 tcacagttac ccgtattggt aaaacatgct ttagaagaaa atcacgcaac taataattat
    39601 catttctatc gacatgtctc tttggatgaa tatcatgcaa ctgataattg gaagactcga
    39661 ttacgaatgt taaaccattt tccaaagccg acttttgaag atataccgct gcttgattta
    39721 gctttatctg atgaaaaagt accggttaga cgtcaagcga ttgtattatt aggtatgatt
    39781 gaaagtaaag aaattttacc gtatttatat aaggggcttc gtgataaaag tcctgttgta
    39841 agaagaacag caggggattg cataagcgat ttagggtatc cagaggcact accagaaatg
    39901 gtgctactat tagatgatcc acagaaaatc gttaggtggc gtgctgctat gtttatcttt
    39961 gatgaaggta atgcagagca gcttcccgca ctaaaagccc atattaatga caatgcgttt
    40021 gaagttaaat tacaaattga aatggccata tctcgtattg aaaacggaga tgaagcactt
    40081 ggttcagtat ggaaacaaat ggcaaacaga acaatttaat tggaggaatt aaatatgaat
    40141 gcatatgatg cttatatgaa agaaattgcg caacaaatgc gtggcgaatt aactcaaaat
    40201 ggttttacaa gtttagaaac gagcgaagca gtatcggagt atatgaacca agtaaatgct
    40261 gatgacacta cttttgtagt tattaactct acatgcggct gtgcagctgg attagcaaga
    40321 ccagctgcag tagcagttgc aacacaaaat gaacatagac ctacaaatac agttacagtt
    40381 tttgctgggc aagataaaga agcaactgct acaatgcgag aattcattca gcaagcacca
    40441 tctagtcctt cgtatgcttt attcaaaggt caagatttag tttattttat gcctagagaa
    40501 tttatcgaag gtagagatat taatgacatt gcaatggact taaaggatgc ctttgacgaa
    40561 aattgtaaat agtacacata aataaatata aaggttaaca cattttataa tattaaaaat
    40621 ggtgtctgtc attgaaaata gagaatatag ttgtattcta tttgtt
//
LOCUS       DALAGF010000014        38369 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.014, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000014 DALAGF010000000
VERSION     DALAGF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 38369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38369
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.014"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            362..763
                     /locus_tag="O2692_000655"
     CDS             362..763
                     /locus_tag="O2692_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HCZ4298296.1"
                     /translation="MKKNITKTIIASTVIAAGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERIDKLMKESDKNLTPYKAETVNDLYLIVKKLSQGDVKKAVVRIKDGGPRD
                     YYTFDLTRPLEENRKNIKVVKNGEIDSITWY"
     gene            723..815
                     /locus_tag="O2692_000656"
     CDS             723..815
                     /locus_tag="O2692_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298297.1"
                     /translation="MLKTVKSTRLLGIKKHTELNSCTPNVRKQC"
     gene            complement(1132..1461)
                     /gene="ecb"
                     /locus_tag="O2692_000657"
     CDS             complement(1132..1461)
                     /gene="ecb"
                     /locus_tag="O2692_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HCZ4298298.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQ
                     TNFKKQVNKKVVDAQKAVNFFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLV
                     LKYNTLK"
     gene            complement(1655..1822)
                     /locus_tag="O2692_000658"
     CDS             complement(1655..1822)
                     /locus_tag="O2692_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298299.1"
                     /translation="MKRIVILENVIVDNPIWLNHVDYLVKSVLLKSIKLRFQEVRKFE
                     ILVKIMNRIHI"
     gene            complement(1950..2453)
                     /locus_tag="O2692_000659"
     CDS             complement(1950..2453)
                     /locus_tag="O2692_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCZ4298300.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(2446..3033)
                     /locus_tag="O2692_000660"
     CDS             complement(2446..3033)
                     /locus_tag="O2692_000660"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCZ4298301.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGDKNV"
     gene            complement(3045..3845)
                     /gene="racE"
                     /locus_tag="O2692_000661"
     CDS             complement(3045..3845)
                     /gene="racE"
                     /locus_tag="O2692_000661"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCZ4298302.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(4081..4896)
                     /gene="sdhB"
                     /locus_tag="O2692_000662"
     CDS             complement(4081..4896)
                     /gene="sdhB"
                     /locus_tag="O2692_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HCZ4298303.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(4896..6662)
                     /gene="sdhA"
                     /locus_tag="O2692_000663"
     CDS             complement(4896..6662)
                     /gene="sdhA"
                     /locus_tag="O2692_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCZ4298304.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(6714..7328)
                     /locus_tag="O2692_000664"
     CDS             complement(6714..7328)
                     /locus_tag="O2692_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HCZ4298305.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(7652..9433)
                     /gene="uvrC"
                     /locus_tag="O2692_000665"
     CDS             complement(7652..9433)
                     /gene="uvrC"
                     /locus_tag="O2692_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCZ4298306.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            9599..9694
                     /locus_tag="O2692_000666"
     CDS             9599..9694
                     /locus_tag="O2692_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298307.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(9757..10071)
                     /gene="trxA"
                     /locus_tag="O2692_000667"
     CDS             complement(9757..10071)
                     /gene="trxA"
                     /locus_tag="O2692_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCZ4298308.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(10244..12592)
                     /locus_tag="O2692_000668"
     CDS             complement(10244..12592)
                     /locus_tag="O2692_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCZ4298309.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEDRTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(12602..14314)
                     /gene="polX"
                     /locus_tag="O2692_000669"
     CDS             complement(12602..14314)
                     /gene="polX"
                     /locus_tag="O2692_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HCZ4298310.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNT
                     KLKK"
     gene            complement(14387..14908)
                     /locus_tag="O2692_000670"
     CDS             complement(14387..14908)
                     /locus_tag="O2692_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCZ4298311.1"
                     /translation="MVIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(14909..15175)
                     /gene="zapA"
                     /locus_tag="O2692_000671"
     CDS             complement(14909..15175)
                     /gene="zapA"
                     /locus_tag="O2692_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCZ4298312.1"
                     /translation="MTQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
     gene            15545..16483
                     /locus_tag="O2692_000672"
     CDS             15545..16483
                     /locus_tag="O2692_000672"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCZ4298313.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSEELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAALHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL"
     gene            complement(16654..19056)
                     /locus_tag="O2692_000673"
     CDS             complement(16654..19056)
                     /locus_tag="O2692_000673"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCZ4298314.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSEAQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELDLSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGVGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQDKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPILAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(19056..20114)
                     /gene="pheS"
                     /locus_tag="O2692_000674"
     CDS             complement(19056..20114)
                     /gene="pheS"
                     /locus_tag="O2692_000674"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCZ4298315.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEDKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(20495..21235)
                     /locus_tag="O2692_000675"
     CDS             complement(20495..21235)
                     /locus_tag="O2692_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCZ4298316.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
     gene            complement(21475..21582)
                     /locus_tag="O2692_000676"
     CDS             complement(21475..21582)
                     /locus_tag="O2692_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298317.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(21619..21942)
                     /gene="isdG"
                     /locus_tag="O2692_000677"
     CDS             complement(21619..21942)
                     /gene="isdG"
                     /locus_tag="O2692_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HCZ4298318.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(21961..22695)
                     /gene="srtB"
                     /locus_tag="O2692_000678"
     CDS             complement(21961..22695)
                     /gene="srtB"
                     /locus_tag="O2692_000678"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HCZ4298319.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDRIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(22757..23722)
                     /gene="isdF"
                     /locus_tag="O2692_000679"
     CDS             complement(22757..23722)
                     /gene="isdF"
                     /locus_tag="O2692_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HCZ4298320.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(23735..24613)
                     /gene="isdE"
                     /locus_tag="O2692_000680"
     CDS             complement(23735..24613)
                     /gene="isdE"
                     /locus_tag="O2692_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HCZ4298321.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(24610..25686)
                     /gene="isdD"
                     /locus_tag="O2692_000681"
     CDS             complement(24610..25686)
                     /gene="isdD"
                     /locus_tag="O2692_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HCZ4298322.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            complement(25686..26369)
                     /gene="isdC"
                     /locus_tag="O2692_000682"
     CDS             complement(25686..26369)
                     /gene="isdC"
                     /locus_tag="O2692_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HCZ4298323.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            26578..27630
                     /gene="isdA"
                     /locus_tag="O2692_000683"
     CDS             26578..27630
                     /gene="isdA"
                     /locus_tag="O2692_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HCZ4298324.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
     gene            27833..29770
                     /gene="isdB"
                     /locus_tag="O2692_000684"
     CDS             27833..29770
                     /gene="isdB"
                     /locus_tag="O2692_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HCZ4298325.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEE
                     TGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEV
                     KAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDG
                     TQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVK
                     DYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAE
                     KLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQN
                     VQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYW
                     KDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVD
                     KEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPS
                     VEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVV
                     QTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLP
                     LMALLALSSIVAFVLPRKRKN"
     gene            complement(29924..30097)
                     /gene="rpmF"
                     /locus_tag="O2692_000685"
     CDS             complement(29924..30097)
                     /gene="rpmF"
                     /locus_tag="O2692_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCZ4298326.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(30177..30734)
                     /locus_tag="O2692_000686"
     CDS             complement(30177..30734)
                     /locus_tag="O2692_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCZ4298327.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            30861..32000
                     /locus_tag="O2692_000687"
     CDS             30861..32000
                     /locus_tag="O2692_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCZ4298328.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            complement(32062..32544)
                     /gene="coaD"
                     /locus_tag="O2692_000688"
     CDS             complement(32062..32544)
                     /gene="coaD"
                     /locus_tag="O2692_000688"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCZ4298329.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(32546..33088)
                     /gene="rsmD"
                     /locus_tag="O2692_000689"
     CDS             complement(32546..33088)
                     /gene="rsmD"
                     /locus_tag="O2692_000689"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCZ4298330.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            33158..33547
                     /locus_tag="O2692_000690"
     CDS             33158..33547
                     /locus_tag="O2692_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298331.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            complement(33550..33804)
                     /locus_tag="O2692_000691"
     CDS             complement(33550..33804)
                     /locus_tag="O2692_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCZ4298332.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            34043..34969
                     /locus_tag="O2692_000692"
     CDS             34043..34969
                     /locus_tag="O2692_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCZ4298333.1"
                     /translation="MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     NLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            complement(35032..35466)
                     /locus_tag="O2692_000693"
     CDS             complement(35032..35466)
                     /locus_tag="O2692_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCZ4298334.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(35482..36519)
                     /locus_tag="O2692_000694"
     CDS             complement(35482..36519)
                     /locus_tag="O2692_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCZ4298335.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            complement(36846..37307)
                     /locus_tag="O2692_000695"
     CDS             complement(36846..37307)
                     /locus_tag="O2692_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HCZ4298336.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            complement(37332..38243)
                     /locus_tag="O2692_000696"
     CDS             complement(37332..38243)
                     /locus_tag="O2692_000696"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCZ4298337.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLFGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
BASE COUNT        12633 a         7035 c         5269 g        13432 t
ORIGIN      
        1 ttgttttata aaaaatatat atcaaaacag ctatatgtat aacaatttgg agtgtttgtc
       61 tatccaactt agcaaacaaa atgtagagaa atgtaaatat cgttttagca ataaaaatta
      121 ttcaatcata gttgagtgac gtttactact attattatac tatcaacaaa ttcaataata
      181 cctcaaataa aaataatacc atcaaattca tcattattct ttttcgattg cattaaacct
      241 tattaatttg cacttaattt taattaatga agtgttaact ggtaaatgta tgaaaccgtt
      301 tgtaaaatag cagtgtactc gcattagtaa gtacaaattt attttacagg gagaacttat
      361 tatgaaaaaa aatatcacaa aaactattat cgcatctaca gtaatcgctg caggtctttt
      421 aactcaaact aatgatgcta aagctttctt tagttatgaa tggaaaggtt tagaaatcgc
      481 aaaaaattta gcagatcaag ctaaaaagga cgatgaacgt attgataagt taatgaaaga
      541 gtctgataaa aatctaactc cttacaaagc tgaaactgtt aatgatctgt accttattgt
      601 taaaaaatta agccaaggtg atgtgaagaa agcagttgtc agaattaaag atggtggtcc
      661 tagagattac tatacttttg acttaactcg tcctttagaa gagaacagaa aaaatattaa
      721 agttgttaaa aacggtgaaa tcgactcgat tacttggtat taaaaaacat actgaattaa
      781 atagttgtac gccaaacgtt agaaaacaat gctaacgtat tggcgtgctt ttttatttta
      841 agtaacttcc aatttattta gcatctttac gactgtttaa taaagcacgt atgattaaca
      901 cggttgccac tatatccgtt acaattttta tgattgttaa tacattcgat ttccttttca
      961 caatagccac ctcgcttgtt caaaacataa acaactattg cattcacttt taagtaatta
     1021 tttatattat ttatcccaat aagctcacca ttcaaataaa cacaatatta taaaataatt
     1081 atacctttgg atatagcaaa aagccacact ctatagcatg gcttctatca attattttaa
     1141 agtattatat tttaaaacta gatcgatttg tctttgtaat tttttctttt cataactgtg
     1201 ttggaaatga attaaattaa cagctctttg tgctttacgg tgtgttgcaa cagttcttgt
     1261 acgtttgaaa aagtttacag ctttttgtgc atccacaact tttttattta cttgtttttt
     1321 aaagtttgtt tgatactgat catatttttt agcagcttca acgtttttag tttgtgcatg
     1381 agattcaccg gcaaacgttg ataccgttaa actaatagca gctatgctta aaattgattt
     1441 cccaataaaa ttctttttca taattaataa cacctatcct caaatattta ttttgtatat
     1501 atttataata atagacagat acaaaaaact acattaacta tttattaact aaacattaat
     1561 atttgtgtca ctacatttct gaatttcaag aattttgcta ctccatattt taaccatcaa
     1621 attattgata taatttatga ctttagaaat ccttttaaat atgtatacga ttcatgattt
     1681 taactaaaat ttcaaatttc ctgacttctt ggaatcttaa ttttatactt tttaataata
     1741 cacttttaac caaataatca acatgattca accatatagg gttatcaaca atcacattct
     1801 ccaaaattac aattcttttc ataacataaa tgtgttattc attccaatta tcttataaaa
     1861 ttgtcagaaa tacttttaaa caaacattca tgacacgacc tcctagttaa atcagtctat
     1921 aaactaaaaa aagccatagc tacttctatt taaaaagtag tatgactgat tgtttcgtga
     1981 tgtcgatttt tgaaattgat ggtgccatgt aaagtttgat catcaataat aacttcagca
     2041 tatgtttctt ccattgaact tctagattga gatatacttc caggattaat aacatgaaca
     2101 ccattaatat actcatattt tgccacatgt gtatgtccat aaaatgcaaa caaacaacct
     2161 aattcaagtc ctttttcagc taataaatct cttgttcgat tgacttgata taaatgtcca
     2221 tgagtataaa atgctttcac gtcattatat tttgcgaccg cttcattttc aaattctggg
     2281 taaaaatcac aattgccttt tactctatta aataagctaa gttccgtatc atcatacgcg
     2341 aattctgaat ctcctaaatg taaatataca tctgcatctg ggtgcatttc ataaatttga
     2401 tataaaacgc ctgattcagt atggttatca ctcacaataa tccatttaga catttttatc
     2461 accttcaaga aaagcttgaa gtaaattaat cgcatttcgt ctatgactaa tttgcccttt
     2521 ttgttctttt gaaagttgag ccatggtttt atctaatttc ggtacataaa atatcggatc
     2581 atatccgaaa ccattttcgc catattttcc atctgcaatt tcacctgaaa cagtaccttt
     2641 aaatactttt gtttccatat cagggccact catacttatg acacaaacaa attgcgcacg
     2701 acgatcagtt gtattaccaa gcttatttaa taatttttca atatttgctt catcgctttt
     2761 attttcacca gcataacgtg cagagtatat acctggctca ccatttaatg caaaaacttc
     2821 tagtccactg tcatcagcta tgaccgtttt attcaatgct tttgcagcag cttctgattt
     2881 taatatagca ttttcttcaa atgttgatcc tgtttcttcc acatcaaaat ctggtattag
     2941 ttctgaaata cctattacgt ggtaatctgg aaatattact ttaaagtcat ttattttccc
     3001 ttgattattc gatgcaataa caatctcttt cattaaaaat cctcctagtc attcactgat
     3061 atacgttcca cattgacaga taaatttaac cactctttga taatgttagt aatgtgagta
     3121 ggatcacctg ttgcaaaaaa tcgatgatct ggatgttcag tataacttgc atgttcatta
     3181 ctaaatgtta gcaatgcact aacttcacga gccgtttcta atccagacga aatcactgtc
     3241 tttttaccac caaaataatc atagataggt ttatagagca atggatagtg ggtacatcct
     3301 aaaatgacag tatcagactc actattacgc caacgtttca gtgtttgatg aatgacaatg
     3361 cttgtaattg ttggatcact atatctcatt tgttctacaa gtggcacaaa acctggacag
     3421 gcaacgccat gtacctctac atgtggattg atacgtttaa tatgcgtacg atatgcttca
     3481 gatttaattg tgccttccgt tcctagtact aatacatttt gatttctagt agtcattatt
     3541 gctgttctag cacctggttc aattacgcca atcactggga ttgataaggt cttttgtaaa
     3601 tattctaaag ctacagcagt tgctgtatta caagcaatca cgagcatttt tatatcaaat
     3661 tccattaatt tacgagcgat ttcaactgta tattgtttta cttgttctcc tggtcttggc
     3721 ccatatggac atcgtccaat atcacctaag taataaatcg tctcatttgg caactgacgc
     3781 ataatttctt tagctactgt caaacctccg acaccagagt ctattacacc tattggttta
     3841 ttcatattcg gtcatcctta cttttaattt aatttttagt ttatcataac taagcattgg
     3901 attttagtat tatgcactgt gtttaccatt tttgtcatta taatatttat tttaaatcag
     3961 cccactatca tattgtcatg taatctgctt attaaaaaaa tcccttccaa gttattgtgt
     4021 atctccattc aatttaattt tgaaaggaac ataacatttt aactcaaaag ggattaattt
     4081 ttattctact tcatggtctg aaccaaagaa tgatttaaac atgtggaatg ttgtttctct
     4141 gttcattgct gcaatggatg ttgttaatgg aataccttta gggcaagcat taacacagtt
     4201 ttgtgaatta ccacactgct gtaagccacc agtacccatt aatgcattta aacgttcatc
     4261 tttagtcata gatcctgttg ggtgcaaatt aaacaaacga acttgcgaga ttgcttgtgc
     4321 accaacgaat ttattatttt cagtaacatt aggacaaacc tctaaacata caccacatgt
     4381 catacattta gataattcat aagctgtttg acgttttttc tctggcatac gtggtcccgg
     4441 acctaaatca tacgttccat caattgggat ccatgctttc atacgtttta agttatcgaa
     4501 cattctagaa cgatcaactt gtaagtcacg gataactggg aaagtattca ttggctctaa
     4561 acgaataggt tgttctaatt gatcaacaat cgcagaacaa gattgtcttg cacgaccatt
     4621 gataaccata gaacatgctc cacatacttc ttctaagcag ttcatatccc agacaacagg
     4681 tgttgttttt tcacctttaa tattaactgg gttacgtcta atttccatta aacaagcaat
     4741 gacgtttaaa ttttcacgat atggaatttc aaatgtttct tcataaggct tagaatcact
     4801 tgtatcttgt cgtttaataa ttaattttac tgttttttgt ttcggtttag attgtgtttc
     4861 atgttgtgga gtgtttttca ctgattgttc agtcattatt ttttaccccc tttagactta
     4921 cttgtgtaat cacgtttacg aggtggtatt aaactcacat cgacgtcatc ataagtaaac
     4981 tgcggttttt caaatgcgcc ttggaatgag gccattgtcg tttttaacca ctcttcatca
     5041 ttacgctctg ggaattctgg tttataatgg gcaccgcgtg attcgttacg gttatatgca
     5101 ccaatcgtaa taacacgtgc aagtactaac atgttccata gttgacgggt aaagaatacc
     5161 gcttggttac tccaagtttg agtatcttcc atatcaatat cttcataacg tttcatcaat
     5221 tcaacaatct ttttatctgt ttctaacagt ttttcatttt cacgaacaac agttacattt
     5281 gctgtcataa tttcaccaag ttcacggtgt aatttatatg cattttctgt accgcgcata
     5341 gctaataatt tatcaaaacg ttcttgctct tcagctttac gcttttcaaa aatactttcg
     5401 tccatatcag tatatgatcg atcaatattt gaaatataat caatcgcgtt tggacctgct
     5461 actgtaccac cataaatcgc tgataacaat gaattggcac ctaagcggtt accaccatgt
     5521 tgagagaagt cacattctcc agctgcaaat aaccctttaa tatttgtcat ttgatcataa
     5581 tctacatata gaccacccat tgaatagtga acagctggga aaatcttcat tggtactttg
     5641 cgtgggtcat caccagtgaa tttttcataa atctcaatga taccacctag ttttacatct
     5701 aactcatgtg gatctttatg tgacaaatca agatatacca tgttttcgcc atttatacct
     5761 aatttttggt taatacatac atcgaaaatt tcacgcgttg cgatatcacg aggtactaag
     5821 ttaccataat caggatattt ctcttctaag aagtaccaag gcttaccatc tttatatgtc
     5881 caaattcgtc caccttcacc acgtgctgat tcactcatta gtcgcagttt atcatcacca
     5941 gggattgcag taggatgaat ttgaatgaac tcaccattag cataaatagc gccttgttgg
     6001 taaacaatgg aagccgctga tcctgtatta atcattgagt ttgttgtttt accgaaaata
     6061 ataccagggc cacccgttgc cataataact gcatctgaac caaatgtttc aatctcagca
     6121 gttgtcatat tttgtgcaac gatacctctt gcactatcat cgtcaccttt aactatgcca
     6181 aggaattccc atccttcata cttcgtaact aatccatcta cttcatatgc acgaacttgt
     6241 tcatccaatg catataataa ttgttgtcca gttgttgccc ctgcatatgc tgttctgtga
     6301 tgtaatgtac caccgaaacg tctaaaatct aatagacctt catttgttct attgaacatt
     6361 acgcccatac ggtctaataa atgaataatt ttaggtgctg cctctgtcat cgctttaaca
     6421 ggtggttggt ttgcaaggaa atcgccacca tacactgtat catcaaagtg aatccaagga
     6481 gaatcgcctt cccctttagt attgaccgca ccattaatgc caccttgggc acaaacagag
     6541 tgcgaacgct ttactggtac aactgagaac aaatctacat gtgcaccttt ttctgccgct
     6601 ttaattgttg acattaagcc cgctaggcca cctccgacaa caataagatg tttctctgcc
     6661 ataaaaattt cactccccta aattttcaat ctatatttgt taaatgcgat gtattacata
     6721 aaggcaataa ttgcagtaac accaatatac gaaataacta aaaatacgat taatgaaacc
     6781 catgtaaata ctcgttgtga ttttggagat tgaagtccac cccaagtaac taagaatgac
     6841 cataagccat ttgcaaagtg gaacacaaca gcaataatac aaataatata aaatattgcc
     6901 catccaggat gttgcaatgt ttcgtgcatt aaatcgtaat tcacttcttt gccgtaaaat
     6961 gctttttgta aacgtgtttg ccataaatgg ataccaataa agataaatgt taagatacca
     7021 ctcactcttt ggaagaagaa catccagttt ctaaaaatcg agtaatgtcc aacattttct
     7081 tttgctgtaa atgcaatgtg tataccaaac aaaccgtgat ataacaacgg aatgtatata
     7141 aataaaaatt ctacaataat tagaaatggt aatgattcca taaagttaga tgccttatta
     7201 aacgcttcag caccttgtgt tgcttggtga ttcactaata aatgaacgac caaaaatgca
     7261 cctattggga taatacctaa taacgagtga atacgtctta gataaaattc attttttgat
     7321 tgagccaaaa ggagtccccc ctgtgaacga atatttaatt tattgagcta tttatattaa
     7381 acgtacgctt aaccccctaa agtgatatcg tttctagcgt tgtcattatc attaagcgaa
     7441 acattttaaa gacaaataca cactgcacga tcaccaaact gcatgtcgaa caatgtaaca
     7501 tttggattcg atatttaaaa ttgcttgtga tgataaactt tctcatttag aaaacgcttc
     7561 cacgtacatt caaaaaaata actttgttaa ccatattgta acattatttc atatattttg
     7621 gggcatgaga atgattctca cgcccagtaa tttatttatg caattgttca tgtaggttct
     7681 ttgcgacgtt ttcaggaata cctatatttt taaaatcttc aagtgtagct tccttcattt
     7741 tcttgattga accgaatgaa cgcaataata atgttttacg tttgttaccg ataccatcta
     7801 tatcatcaag tattgatttc aagcctgtct tttgacgtgt ttgtctatga aatgtgattg
     7861 cgaatctgtg aacctcatct tggatacggt gcaacaaata aaatgcctgg ctatttttct
     7921 tcagtggtac aatttctgca ctagcgccat ataataattc agatgtttgg tgtttatcat
     7981 ttttctgcaa acctgcaaca gggatatcaa gacctaattc gttttgtagc acatcaataa
     8041 ccccgttcat atgtccttta ccaccatcta ctattattaa atcaggtaat ggtaatcctt
     8101 cgtttaaaac gcgagaatat cgtcgtctta ctacttctct cattgatttg taatcatctg
     8161 gacctttaac cgttttgatt ttatactttc tataattttt cttatctggt ttaccgtcga
     8221 caaatgtaac cattgctgac actggatcca caccttgaat attagaatta tcgaatgctt
     8281 caattctaat tggtgtttga attcccattt gtgttccaag ttcttcaata gctttaatcg
     8341 ttctggactc atcacgtgat attaattcaa atttattatt taaggatact ttagcgttat
     8401 gtgcagctag gtcaaccata tcttttttgg gacctcgcgc gggttgaacg attttagtgt
     8461 ccacaacaga ttgaatcatt tctttatcca aattacgtgg tacatgaact tccttaggta
     8521 aaatatgttg gtttaagcta taaaattgtc caataaatgt ataaaattct tcttcttctg
     8581 tttgctgtaa tggaatcatc gttgtatctc gctttatcat attaccttgt cgtataaaga
     8641 aaacttggat acacatccat cctttatcaa cactataacc aaagacatca cgaatcgttt
     8701 tatctgatga cataattttt tgtttgtttg tcagattttg aatatgttga attaaatctc
     8761 tatattcttt agcccgttca aaatcaagtg attcacttgc agttaacatt cgctcttcta
     8821 aactttttaa aattgttttg tcttccccat tcagaaaatc agtaatttcc ttcgtcattt
     8881 gtgcgtattt actcaaatca acgtcatata cacatggtcc taaacattgt ccaatatggt
     8941 aataaagaca taatttatct ggcatcttat cacatttgcg atatggatat attctgtcta
     9001 ataacttttt agtttcttga gcagaatatg cattcggata cggtccgaaa tatttgccag
     9061 taccttgttt tacagttctc gtcactagta gtctaggata tttctccttc gtaattttaa
     9121 taaatggata acttttatca tcctttaata atatattata tcttggttga tattgtttaa
     9181 tcagattcaa ttccagtaaa agtgattctg tttcacttga cgtgacaata aactcaaagc
     9241 gacgtatttc accaaccagt ctcgttgttt tagcatcatg agcacccgta aaatatgatc
     9301 gcaatcgatt tcttagcttt ttagctttgc caacatatat cacttgatca ttacgatctt
     9361 tcattaaata gcatcctggt tccataggta cgacatttaa tttattttta attcgttgct
     9421 tatagtcttc caaaaatatc cccccttcta attttaaaat gaatgatgct acaaaagtgc
     9481 ttcaaattta tcaccactaa ttctagttaa taatcaacat cttcgagtat gacttttcat
     9541 gcacatcatg taatattgaa catggtttta tcaaaccaac tgctacaaat ctatcagcat
     9601 gctagattat catttaacgt tcatagattt attaatccca ccattctcaa gtcgtaaaca
     9661 atatgatgac ttatcattca actttttata ttaaaaacac aaacaaaaac tggagcaatt
     9721 cattaaagaa atgccccagt cgtcattggt tgtaacttat aaatgtttat ctaaaacttc
     9781 agctaagttt tcttttggtt ggaaaccaac aactttatca actggttgac cgtctttaaa
     9841 gacgattaat gttggaatac tcatcacttc atatttagct gcagttgatg gattttcatc
     9901 aacatctaat tttaaaatgt cagctttacc ttcatagtca gctgctaatt cttctaatac
     9961 cggagcgatc attttacatg gaccacacca tgttgcccaa aaatctacta gttgtacacc
    10021 agattctact tttgaatcaa aatctgcatc tgttactttt acgattgcca taaatgccaa
    10081 tcctccttaa ttattattat gatgttcaaa ttatatcaga taacatgctt catgtatttc
    10141 tatttgctca atttttatgc ttatttcaaa taacatgttt gaaatgtatt cttgttgctt
    10201 tacattattt taactgcagc tatttaattt atcaaattat aatttatttt agtgttgcaa
    10261 cggtaacgcc aaatccacct tcgcttggca taccacctct aaagtcacta acacttttat
    10321 gctttttcaa atgttgttgt acaccttttt gaagtgcacc tgtaccttta ccatgaatga
    10381 tataaacttg ttcgtaatta cttaaaacgg cttgatctaa atattgatct agttcaatta
    10441 aagcatcctc ataacgatag cctcgtaagt caagttcagt tttaattgtt tgacgatttt
    10501 gacgtgtaac cattttcgtt ggcttaactt tttctttttg ttttttctct aaatcttcaa
    10561 taggtaactt cattttaata attcccattt gaacaattgc ttcttcatca ttgacaattt
    10621 ctaaaacttc acccttttga ccgtaagata atacttttac ttcatcacca gcaacaattt
    10681 tatcgtattt ttgcttttgt acattttgct ttatagattt cgcttcataa tgatcatcta
    10741 atcgtttctt cttatcaatc aattcatgtt ctttaacatc tgcacctttt tgttcacgca
    10801 attgtcttaa gtctttaata atatcgtcag cttcttttgt tgcagcctta atcttctgat
    10861 ttgctttttc tttcgcttcc tctattagag acttttcata attttggaat tgttgatact
    10921 gcttagataa atcatcatgc acttgctccg cttcttttac aagacggtcc agttctaacc
    10981 tctgtgtctc tacacgtttg taattacgct ctaatgattc aatcatttca tttatttctt
    11041 tttcatcagt accaatcatc gtcttagcct tattaataat attcaaacta agacctaact
    11101 ttttagaaat gtcaaaagca tttgaacgac ccggcacacc cattaataac ttatacgttg
    11161 gactcaaagt atctacatca aattctacac tcgcattcat aacgccttct cgattataac
    11221 tatatgcttt aagttcagga tagtgcgtcg ttgccattac tagagaacca atttttctaa
    11281 catgatctaa aatgctcatt gctaatgcag caccttcact cggatctgta cctgcaccta
    11341 attcatcaaa taaaactaaa ctatgtttgt ctgcatgctt taaaatttca actatattcg
    11401 tcatatgaga tgaaaaagtt gataatgatt gttctattga ttgttcatct ccgatatcgc
    11461 aatatacatt tttaaataca cttaactgac taccatcaag tgtgggaatc aacaatcctg
    11521 attgagccat aacaataatt aaacctaatg tttttaatgt tacagtttta ccacctgtat
    11581 tcggtcctgt aataattacc gtttcaatat cttccataaa ttcgatggta ttagctacaa
    11641 cagtctcacg atttaataat ggatggtatg ctttaggtaa atatacagta cggtcctctt
    11701 taaatatcgg ctttgttcct ttaatacttc tactatatct cgcttttgcg attaaaaaat
    11761 ctaactgacc catgacttgt tctgccacaa gtagtgcatc tttgtccgca gccacataac
    11821 cagttagttg cgttaaaatg cgttcttttt caattgcttc gtcatgacgt aatcgactaa
    11881 tttgattatt catttcaaca actgatgatg gctcaatata caatgtttgt cctgaagcag
    11941 attgatcatg tacaatccca ttaaaatctt gtcgatattc agctttgaca ggtataacgt
    12001 ttctttcatt cctaactgtt acaatagcat ctgataattt tttctgattt gcttggcttt
    12061 taacaatacg gtccaaattt tgtctaatac gttgattcgt gctagaaatt ttacttctaa
    12121 tcccttgcaa ttcataactc gcattatcat ataaatcata cgtatcgcat gtttcattta
    12181 tttgttgaaa aagatcagtt aacacaggta attgattcat cttgtcatct aatattgggt
    12241 atttaacacc ttcatcttct tcaaccaatt gattataaaa tgtcttgaat tgattttgta
    12301 cttgaattaa tctttttatc aagttaagct ctgatacatt taaaacgccg ccaatatcag
    12361 cgcgatgaat gaatgctgat actttagata agccactcaa gcttggtaaa cgatgcttat
    12421 tatagatttg agcaatctca tccgtttctt ccatttgaaa aacaaccgtt tcaaaattag
    12481 tagctggcat catttgattg accttttcca agcctaagtc actaatagtt tcattggcaa
    12541 cgagtgattt tattttttca aattctaaga cgtctaatgt tttttgtctc ataaaatccc
    12601 tctatttctt aagttttgta ttattttcaa taaagtcttt aaaagcttca cgcgataatg
    12661 cgttaatcac tctatctttt gttacaaatc ctttttgcgc agttgctacg ccataattca
    12721 taaaatctaa atgatttgta tgatgcgcat cagtgttaat agttaatttc acatttggat
    12781 atttacgaac gatatcagcg ctcagatcca gtcgatgtgg attggcatta atttctaata
    12841 ctgtattcgt ttcttcagct aatgccatta attgttcaat attcggttta taaccatctc
    12901 ttctacctat aatacgccct gttggatgcg ctatatgtcg cacgtatgga ttgcgacatg
    12961 cattagctaa tcgttccata atttgttctt ctgattggtt aaagctttga tgaatagctc
    13021 caattacata atcaagttgt gctaaaattt catcatcata atccagcgag ccatcaggta
    13081 atatatccat ttctgtacct gaataaatat caatttcact atattcttta tctaaagcct
    13141 taatttcttc gttttgtctt aaaagtcttt ctacttgtaa gccattagca acacgtaaac
    13201 tttgtgaatg atcagtaatt accatgaatt tataaccttt tgcgatattt gcttctacca
    13261 tgtctcgaat agaaaacgca ccatcactat acgttgtatg catatgaata tcaccattaa
    13321 tatcatctat tgtaatgata ttacttagat ctttatcaaa ttcgctacca tcttctcgca
    13381 tagcaggtgg tataaaattc acattaaaat gttcatatat cttggcttca ctatcatatt
    13441 gaattaatgt accatcagct tgttcaattc catattcact tactttttca tcacgtgctt
    13501 tagcaagttg tcgaattctt atattatgtt cttttgaccc agtaaaatgc tgcaatgtat
    13561 gataaaaagc acttggttca attaatcgaa aatcgacacc aatcgtttca tcatcatacg
    13621 ctaattctaa tgaaactttt gtgttcccca ctgcaacttc ttttacttta ttgggaatat
    13681 ttaataattg ctgctgcact gcttttgggt tatcggtact tattatgaaa tctaaatctt
    13741 tgctcatttc tttaaaacga cggaagcttc ctgcagatga atattgatcg atataattta
    13801 atgtatctat ataatcaatg atttcttgat taagtcttct catttgatca attggatatc
    13861 tatctttctt agcaccaagt tgtttcacag cttctaatat gttttgttcc gttttcttag
    13921 caaatccgct taattcacta acttttccat tttcacaagc aacttgaagt gacgctttat
    13981 caacaatatt caactcttta tatagcttag caattttctt gcttccaagt ccttgaattt
    14041 tcaaaagtgg aataagacct tccggaactt cttcctgtaa ttgctgtaaa tactgagatt
    14101 caccggtctc acggtaatca ttgattactt ctgcaacacc tttaccaatg ccttttaact
    14161 ccgttacatc agatatttca tctaatggtc gttcatctaa ttcaagactt tgagctgctt
    14221 ttcgatacgc tgatatttta aaagtatttt ccccttttaa ttccatataa gtagcaattt
    14281 gttctaatag tttgataaca tctttttttg tcataataac actccataaa aagaagacca
    14341 ggacgtatca ttaatatata cctttgtcct gacctcttat gataatttat aaattaatgg
    14401 tgaaagccga taaatatggg atgtgaaaaa tcaatgaatg actcacaata gatattttaa
    14461 gctgatgttg taatgcttcg ttaggatata atgccaatag atataacgta aagtgtaaga
    14521 caattatcgt cataaacaca ccaactatca ttcccattgc acgactgaaa atatgaatgt
    14581 tttgatacgc tattatttta tcaaaagtta cgataattag atataaaatg aacttacaaa
    14641 acaatgtaat cattaaaaaa gctacaatcg cttcaaatcg attttgtaga tgattaaaat
    14701 gaaacgcaaa agttgtatta aatgctgttg ttttaggata tggaataaat acaattaatc
    14761 tttctacaat agatttgtaa aattgactgg caatccacaa tgatacaatc gttgcactca
    14821 aatgtatcat agataaccaa aaacctcgtc tgaatccaac gatgacaaaa tacacaaaga
    14881 aaatgattat gataaaatca atgaccattt attgctcacg ctgctgcaat ttgtgaattt
    14941 gttgtttcaa acgtcgattt tcttcttcta gtagtacttt ttcatgcata atattcacag
    15001 cagttagtat tgcttttctt gaagtatcta aacctgctgc tttataccct aattctttta
    15061 ttttatcatc aactaaatgt gctacatatc gtatgtgctc tgggttatct tccccaacaa
    15121 ttgtaaaaag ctgatcatta attgatacat ttaccttgtt tttaaactgt gtcatttata
    15181 atttctcctg atcctttttt taaaatctaa attcacgtta taaaatatga ctggatagtt
    15241 tgtctgaatt tgatactaat attgttatat tgcaattatg ataaaacaac aacacaatct
    15301 ctatagatga cttaatgttc tttttataat gaaataatgt aaagaatttt ctattcaata
    15361 ctttatcatg tttaaattgt gtcactataa cattttcata aacattatac atgaccacta
    15421 tgtattttgt aagtatccgc aattaattct ttacaacata cataaatgtt tctgacgtta
    15481 ttatcattta tgatatgatt atttttgcta aagacaatga aattttatga aaggatttac
    15541 acaaatggcg aatatcgttt ttaaattgtc ggataaagac ataacgacat taatgtcacg
    15601 catttctttt gatactgaga atttacctca aggtatgaaa gcacgtgcaa agtatcaaaa
    15661 tacaactgta aatatttacc aatcaggtaa agtcatgttt caaggtaatc atgcagaagc
    15721 tgtgtctgaa gaacttctac cgcagcattc tcaattgaat actaataaaa ctaaaaagaa
    15781 aaatatggct aattcttttc ttgaacaaac tttaatgtat gatcaattta actgtattgg
    15841 tagtgatgag gctggaagtg gcgattactt tggtccgttg actgtgtgtg ctgctttcgt
    15901 taccaaagag catgtcccta ttttaaaaac tttgggtgta gatgattcaa aaaaattaac
    15961 cgataccaaa attgtagaac ttgctgagca attggttact ttcataccac attcattatt
    16021 aacactacac aatgaaaaat ataatattca gcaagcaaaa ggctggactc aagtaaaaat
    16081 gaaagctgcc cttcataatg aagccatcaa aaatgtactt gaaaaaattg attcgtctca
    16141 attagactat atcgtcattg atcagttcgc aaaacgcgaa gtgtatagcc attacgcatt
    16201 atcagatata ccacttccaa agaaaacaaa atttgaaaca aaaggagaat cgaagtcatt
    16261 agccattgca gtggcaagca taatttctcg atacgcattt gttacatata tggatcaaat
    16321 atcaaaaaat attaatatga cgattcctaa aggtgccggt gctaaagtcg atgtaatcgc
    16381 cgccaaaatt ataaaaaaat acggtctttc acgtttagat accatttcaa aaaagcattt
    16441 taaaaatcgt gaaaaagccc aaaaaatttt aaagcctctt tgatttttaa tcattagagg
    16501 cttttatgca gttgtgcgaa gcatcatctg tattccgctg ttcctctttg actttttaaa
    16561 tgcattggaa ccaaattggt gaacccaaaa ataaaacccc atgttaggga tatcaatcta
    16621 cttcagatat ccttatacat ggggtttaaa tcattatcta ataacagcac cttgttcaat
    16681 taatgctgct tcaatttccg cttgtacttt tgaaacgcgc tcatctgtca atgtttcttc
    16741 tgtgtctaaa taatttaaac gtattgcaat tgatttttta cctttttcta aatgttcgcc
    16801 ctgatataca tcaaagacaa gtgtatcttt taatatattg ccaccatgtg catgaatcgt
    16861 tgataataaa tcagctgctg gaatattttg atctacttct aatgcaatgt cacgagacat
    16921 gcctgggaat ctcggaattg gctggtaatt aatgtaacct accgacacag ccattaatgc
    16981 atcaaaattc aactcaaaaa catacgtacg ttttaaatca ttatcagctg ctaatattgg
    17041 atgtaattca ccaataaaac caacaacttt attctctaat aagatttcag cagtacgacc
    17101 tggatgtaat ccatcaatat cagcacggcg ataactaaat tcaagattta acttttcaga
    17161 tactcgatcc acgacacctt ttgctaaata gaaatcaacc gtttctttct tatcttgcca
    17221 ttgattgact acataatctc cagttaaaat accacttaaa tattcaactt gatctggtag
    17281 ttcaccttct ccattagcaa agaagacatt gccgatttca aataatttta catctttatt
    17341 tttgcgtgcc acattatatg atgccgcttc gattaaatgt ggtaataaac tttgacgtaa
    17401 tgacgcatgc gcttcactca ttggcatcaa taaatcaatt gtttgacgct gttgcatcga
    17461 aaatgcagta gcatcttctt tagaaactaa cgaatacgta atagcttggt ctaatccaac
    17521 accttctaac acttctttaa ccattctagt tttatattgg cgatcagtta gctgaccact
    17581 agtaacttta tcgaagacag gtaacgttga tggaatatcg tcgtatccat atatacgtgc
    17641 aacttcttca attaaatctt ctttaattgt aatatcttta cgacgtgatg gtactagcac
    17701 tgtaataaca tcatcattta tttctgtatc aaaccctagt tgattaaaaa tagtaacaat
    17761 atcattttgt gacaaatcaa atccaatagt gcgattaatt ttatcagcag tgatgtcgat
    17821 tggtgtaata aatgcaccaa gttctcctga agacactcta tcttttagca cttttccgtt
    17881 tgcataagtt tgtaataaat aacatgcacg gtcgactgct tcatctacaa attcagtagc
    17941 tattcctttt tcaaaacgac tagatgattc actgcgtaaa tttaaacgtc ttgatgtatg
    18001 acgaattgaa actggatcaa aaatagcacc ttcaatcact atatttgatg tttgttcttt
    18061 aacttctgaa aaatcgccac ccataacacc agctaatgca attggagttt gtccattagt
    18121 aatgacaata tcgctcgtta ataattcacg ttctgtatca tctaatgttg tcattttttc
    18181 gccttcatta gcttgacgaa caacaatttg ttgtgaacca atcgcatctt gatcaaacat
    18241 gtgcaatggt tgaccgtatt ctaataacac ataatttgaa atgtcaacaa cattattaat
    18301 aggacgtata cccgctttta ttaagcgtgc ttgcatccaa attggcgagg gttcaattgt
    18361 cacgtcgtga acaacacgtg cactataata tggtacttta tcttcatttt caattgtcac
    18421 agtcagttca tcatttgcag ataaatcaag ctcatttgat gttgtctctg gcttagtcat
    18481 ttttgtatta tataatgctg caacttcata agcagtacct atcatactta aagcatctgc
    18541 acgattcggc gttaaatcaa attccattac ttgatcatct aaatataaag cttgtaaggc
    18601 atctgttcct ggaacttggg cttcactaaa tacataaatg cctgattcaa aacttttcgg
    18661 tatatagtta cttgaaatac caatttcttg taacgaacaa atcatacctt ctgaacgttc
    18721 accgcgtaat ttggcacgct taattttaat accaccaggc aatctgccac ctactttagc
    18781 aacaatgaca tattgtcctg catcaacgtt cggtgcacca caaacgattt gtacaggttc
    18841 gtcttctccg atatcaactt ggcaaacatt taatttatca gcatcaggat gtttctcttt
    18901 tgacttaacg aagccgacaa ctaaattttt gatatctttt gtgtagtcaa ttaaatcatc
    18961 cacttcaata cctgtgcgcg taatacgttc tgccaaatta cttacagaat catcgattgt
    19021 tacatattct ttcaaccatt catttgatat caacattaca tgtcacctct atcttctacc
    19081 gctttaaatt gatctaaaaa tctcacatca ttagtataga aatgacgaat atcttctata
    19141 ccatatttca acattgcaat acggtctggt cccataccaa atgcaaatcc agagtactct
    19201 gaagaatcaa aaccagccat ttctaataca ttaggatgta ccattccagc acctaaaatt
    19261 tcaatccatc ctgtgtgttt acacacatta caaccttttc ctttacattt aaaacatgac
    19321 acatcaactt ctacagaagg ttcagtgaat gggaagtaac ttggacgtaa acgaatttca
    19381 cgatcagcac caaataattt cttagctaac aattctaaag tacctttcaa atcactcatt
    19441 ttaacgtttt tatcaacaac taatccttcg atttgtgtaa attgatgact atgtgtcgca
    19501 tcatcagagt cacgacgata cactttacca gggcaaataa ttttaactgg accttgacca
    19561 tgacgtgatt ccatcgtacg tgcctgcact ggtgatgtat gcgtacgtaa taaaatttca
    19621 tccgtaatat agaaactatc ttgcatatca cgtgcagggt gtgatttagg taaattcagc
    19681 atttcgaagt tataatgatc ttgttcaact tcatatccat tcacaatttc ataacctaaa
    19741 cctaagaata agtcttcaat ttcttctatt gtacgtgtta atggatgctt tgaaccgatt
    19801 tcaatatgac gacctggtaa tgatacatca attgtttctt cagccaattg cttatttaat
    19861 ttttctttaa ctaacatctg ttgtctttca tctaattcat tttgaattgt ttgacgcaat
    19921 tcattcactt tttgaccaaa cgcaggttta tcttcattcg gcaaatcctt catcaatttc
    19981 attagtccgc taactgaccc ttttttacct aagtatttca ctttaacttc ttgcagtgca
    20041 cgttcatcat ttgcttcatt aatatctaca agcgcttgtt gttttaactc tgacattgtt
    20101 tgttgttcag acatatgtca ctgacctccc tattcgaact attcattttc tatttatcat
    20161 atttgttgtt taactaaaaa aagactttca tcctcttaaa aaataaggga cgaaagtcat
    20221 ttccgtggta ccacccaaat ttatgcctaa gcatacactt gtttaatatt gataacggat
    20281 tgtctttccg acttaaatct ctttaagtaa ccaaaaaggt gaaaagactt taaataattg
    20341 gaataacttg cagtcacgat tattctccct aaaacaatta tttatataaa tgtccatgcc
    20401 tttttattgg tttatattca attgtgtatg cattcacgta gaaatgttct gttaaacaat
    20461 taattataat tatatcggaa aactttcaac acggtcaacc tttcaaatga taaagtaaaa
    20521 tactacctgc aatcgctaca tttaaacttt cagctttacc ataaataggt atgattaaat
    20581 tttgtgtagt atgtgctaat aattcaggat taacaccttc tccctcatta cctaatagta
    20641 atgcaaaaga atcacttgaa gtaacttctt tgtatgccac tgcgttttca agtgctgtac
    20701 cataaacagg accattaaat tgagtaataa acgtatcgag atcttgtgtc ataactggca
    20761 aatggaaaac actaccttga ctcgctcgca acactttatc ttgataagga tctgtcgtac
    20821 ccttctccat tattacagca tccattccag cagcatccgc agttctaatt aatgtgccta
    20881 aatttccagg atcttgaaca cgatcgatga gcaatacttg ttgtgcttta gaaatatcat
    20941 aatgcggctt ctcaatgatt gcgaaaaacc cttgtggtgt cactgtacct gataaagatt
    21001 cagcaacttt catgtttatt tcaaaaactt cttgcgcgta tgcgataatt tgctgatcta
    21061 atcttgccgg ttcaattgca aataattgtg taattacaat tccactttga taagcttctt
    21121 caattaaatg cacaccttca attaaagcta atccagtttt atccctctca cgtttctttt
    21181 ttagcttgtt cgcttgttta attctattat tttgtgcaga agtaatttgt tccattgata
    21241 gctcctcgct ttatttttaa aaataaaaat attaatcatt aataagatga aaacatttga
    21301 ttgtatagtt aatattaatt aatcgctttt atcactcata atatttcaaa ttgtataaat
    21361 ttcttttatc gatactacta ctataaatca tacgccccaa aatatcatta ttaattcttt
    21421 tcttcttcaa aataaatcaa aatgatataa ttgatgatta ttttcaaagc acattcaaat
    21481 caaactatgt tttagcaatt tgttgttagc atgtttgtgt tcattaaaaa aacgaccatc
    21541 atcggtatca tgtatggtcg ttacaaaagc taacaatacc aattgtcata acaagtactg
    21601 caacctcttt aaattcaatt atttcatgta actatagcct atatcatatg taattacttt
    21661 gttatttata atcgggctac tttcatcttc atttttactt ctaacatgtt tatgcgctgc
    21721 tttaaagaca tcagatttta accaatccgt aaaagcttgc tttgatttcc aaactgttaa
    21781 aattttcact tcatcaaaat cttcttgttc taaagtttgt gtaacaaaca tgccatcaaa
    21841 gccttctaat gtttcaatcc catgtctcgt gtaaaatcgt tctataatat cttttgctgt
    21901 tccttttgtt aacgtcagcc tattttctgc cataaatttc ataattatcc tcttttctgt
    21961 ttaacttacc ttaattattt ttgcgacaac aacaattctt ttcgtcgttt cactatatgc
    22021 atcttcgcac gttgataaag tcattattct gtcttttacc gttacattaa catctgaatt
    22081 aattacagat ttacgttttg tctcatctaa aaattgttga taatcttgat cattttcaaa
    22141 atctgtacgt atgtaattat ctttagtagt agttttatat gcactaaata cttgcaattg
    22201 atatttacca tatttattgt caaattcaat tatcttgtgt ttttcataaa acgattgctt
    22261 taaataatct tctaacacat caaacatcgt attatcaccg acatggtgcc cgtataaaat
    22321 agtattatga tttaaattct tcaattcatt tctaaaatcc ataaaaatac tacctttacg
    22381 tcgatgttct cgctcaaaat ctaaatttaa ataatcgtga tttgtcttac cttgtagtac
    22441 tggataattt aatgatgttc ctgataattt tatccatcca acaatgtctt tatttatttt
    22501 ttcaagtgat tcaaattgtg gtctcacatg ttcttgatgt ttgctcatca gcatttgaaa
    22561 tttttgttgt aatttctcat aatttgcgcg ttcttgcttg tcttcaatat atgtttgaac
    22621 aattttgtaa ccaaaaatga taataattac aaccaataaa atttgtacaa tagttaaaaa
    22681 tcgcttcatt ctcataaaaa tcctctttta ttaacgacgt ttcttcagtc atcactaaac
    22741 cagttgttgt accgttttag attcgatttc gttgactttg acaaattaag taaattagca
    22801 ttggaccacc gacaatcatt aaaatagcat tggctggaat ttctaaagga ggctgtatca
    22861 ctcgtcctaa taaatcagcc actaacaata gccatgcacc aataactgta gaaaacggaa
    22921 taagtactct gtaattgccc ccaactagct ttctaaccac atgtggcaca ataataccta
    22981 aaaaggctag ttgtccaaca atcgcaacag ttgcacttgc taaaaatact gctaataaac
    23041 ctgttaacca tctgtaacga tcaatattaa aaccgatact tcgcgcttgt atgtcgtcta
    23101 aatttagtaa attcaattta ggggacaata gtaatgttaa tattaatccc aataatgctg
    23161 atactgctaa tatgtatacg tcgctccata ttttcattgt taagccttga ggaattttca
    23221 ttaaagggtt ttgagttaaa atttctaaaa caccatttaa taatacgaat aacgcaacac
    23281 ctactaatat catacttaca gcattgaatc taaatttaga atgcaacaat ataattatta
    23341 aaaatggtat taaacctcca ataaaactta ataatggtaa gtaaaagtac aattgtggaa
    23401 taaacaacat acaaagtgct ctcattataa gtgcacctga ggaaacgcca atgatattcg
    23461 cctctgccaa aggattttgt agtgctgctt gtaataatgc tccagaaact gctaacattg
    23521 cgccaaccat caatgcaatt aatatacgtg gcaatcgcaa atcaatgatt gaatccactg
    23581 cttcattgct accagttgta aattttgtaa ataggtcatt aaatgacaat ttaattgtac
    23641 cggttacaaa cgaaatataa gcagttgcga ttaaaatgac taacaaacat aaaaatagta
    23701 gtttcttttt attttttatc atcatatcaa ctcactattt tttatcctta taaaataaat
    23761 catataattg agtcattgcc ttatcagcat caacatttgc tgtaataccg aatggcactt
    23821 cctctaagtc ataaacatga ttatttttca cagctttaaa atgtttccaa atatcatttt
    23881 gtttaaattc tttttgaaac attttcttaa cttcttcagg cattccgtgt ggtaatcgta
    23941 aaataatatc tggattgata ttcaacaaat tttcagtatt agacgaaata tattgacgat
    24001 ctttcacttt aataacattt tctccacctg ctattttaac taaatcacca atatatgatt
    24061 tatcagttgc tactaaatag ctacccggta cacccattaa tattaatact ttgggatgtt
    24121 tcttttgttt agctgcttta ttttcaattt tttgctttac agaatttaaa tggtcattta
    24181 attcttttgc ttgtgcttta cgattaaatt gatcacctaa ttgtgtaatc gacttttgca
    24241 tcccttttaa actatcaaaa tcataaaagt agcctttcat atttaattgt ttgtaaaatg
    24301 gttgcatttc atctttaatc gttgacacac tcaaaacatg tgttggtttt aactttttaa
    24361 cagcttcaac attcggctcc attggttgac caatttccgg tacatcttta taacgattag
    24421 gcaatgtctt atatgacgtg ggtttgccga caattggtaa atccaatttg tccaatgtca
    24481 ttgtcaatgc aacagttgtt ggtacgattc tgaattcgcc ggatttagtt gattcttgag
    24541 aactggaaga ttgacagctg gttaagataa cgacgcttat cactaaaatg gttaaatact
    24601 ttatgattct caaacttggt tacctctctt tctgctgtat aaatgtgccg aaataagtaa
    24661 aatagttgct aacacaatgc cagctgcaaa taacatatat tgctttttat gatttggttc
    24721 atcaagttct ttaatatcac tagataattg actactattc ttgctatcat catctctatt
    24781 aaaagctggt aacgcttttt ctttttcaac tacttttttc ttttcctttt ttgatttttc
    24841 atctttatgt ttcggttgct ctttgtgatt attttcaacc ttttttattg ttggagggct
    24901 attttcaact tttaatgtac ctgatgcttt aggtcgctca ttttcaatct ttttattact
    24961 gtcttgttgc actttatctt tttgtacagg aatatcattt aatccttttt cagtaaaaag
    25021 gctagtacgt ctttccttag tatctgattt ttgactatct tttttgtcat caatatcttt
    25081 tttaagctga tcttgattat tttcttgatt ttgatgtttt gaaggcactt cttttgcatc
    25141 atcatgttgt acttttttct ctttaaaatg aatagaatac accatttctt tgttatctag
    25201 atttttcttt tcagccaacg ttttaacatg catattgatt ttatccatat gctgttcaat
    25261 taaaattctt actattgtgt catccttttc atcttttata acaaaaggaa attcttcatt
    25321 tttgtcattt tgaaaaacaa gttgttcttt atctatttga ccttttagct ttacttgcaa
    25381 gtaaatttta ccagtcatat ctgaaatcaa ttgcgccttt tcgtcaacta gttgttgttc
    25441 ctcagttaca tttttatttt cttgattaaa aattagtaac tctaacgctt tattctcttt
    25501 tatcttttca tcaaaaattg atgtagttaa ttctgtttta tcttttgctt caggttgatt
    25561 aaatttggct gatggatatt ttatctcatc agccaacacg ctaccaatca ttgttgtata
    25621 tgtaagtata cctaagctaa taatagctat aatagatttt gtagcaattt gtttaacatt
    25681 tctcattatt ccacattgcc tttagatttt tttctaacaa acaatgtgat tgcgattaat
    25741 aatgctaagg atgcaactgg tattgcatat atatatgcag gtgtaccagc atttgtttgt
    25801 ggattttcta ctttgtcttt attcgaacta ttagattcac tttgaccagt agtcgttcca
    25861 tcagatcctt tgtcactacc tgaagcagaa tttttatcat ctttacctgg tgcattagca
    25921 cctgctacat cagttggtcc attaaattta tatgtaatgt tgtaatgatg gtcatatttg
    25981 aatggctttc catttacttt ttcatcgata taaacgtcaa tttttccatc tattttaccg
    26041 ttcaacttac ttacttcaaa ttcagaagtg cgttcatctt tggcagtgtt tttactaata
    26101 atattttctt tatgtccttc gatactcatt ccagtaatcc aatgactgtg gttgacagtt
    26161 atttgaacat acaatttacc atttttctta atgtactttg ccggtttatt aaaatagtca
    26221 ttagcaattg acgtgtcatt ggtattgtat ttgtaaacct cataattcaa agtaccgcta
    26281 tctgcggcat ttgcagaatt actgaatgtc gcgatgatga taattaacgc taaaatcgtt
    26341 gtattaaaaa cttttaaaat atttttcaaa acataatcct cctttttatg attgctttta
    26401 agtctttagt aaaatcataa ataataatga ttatcattgt caatatttat tttataatca
    26461 atttattatt gttatacgaa aatagatgtg ctagtataat tgataaccat tatcaattgc
    26521 aatggttaat catctcatat aacaacacat aatttgtatc cttaggagga aaacaacatg
    26581 acaaaacatt atttaaacag taagtatcaa tcagaacaac gttcatcagc tatgaaaaag
    26641 attacaatgg gtacagcatc tatcatttta ggttcccttg tatacatagg cgcagacagc
    26701 caacaagtca atgcggcaac agaagctacg aacgcaacta ataatcaaag cacacaagtt
    26761 tctcaagcaa catcacaacc aattaatttc caagtgcaaa aagatggctc ttcagagaag
    26821 tcacacatgg atgactatat gcaacaccct ggtaaagtaa ttaaacaaaa taataaatat
    26881 tatttccaaa ccgtgttaaa caatgcatca ttctggaaag aatacaaatt ttacaatgca
    26941 aacaatcaag aattagcaac aactgttgtt aacgataata aaaaagcgga tactagaaca
    27001 atcaatgttg cagttgaacc tggatataag agcttaacta ctaaagtaca tattgtcgtg
    27061 ccacaaatta attacaatca tagatatact acgcatttgg aatttgaaaa agcaattcct
    27121 acattagctg acgcagcaaa accaaacaat gttaaaccgg ttcaaccaaa accagctcaa
    27181 cctaaaacac ctactgagca aactaaacca gttcaaccta aagttgaaaa agttaaacct
    27241 actgtaacta caacaagcaa agttgaagac aatcactcta ctaaagttgt aagtactgac
    27301 acaacaaaag atcaaactaa aacacaaact gctcatacag ttaaaacagc acaaactgct
    27361 caagaacaaa ataaagttca aacacctgtt aaagatgttg caacagcgaa atctgaaagc
    27421 aacaatcaag ctgtaagtga taataaatca caacaaacta acaaagttac aaaacataac
    27481 gaaacgccta aacaagcatc taaagctaaa gaattaccaa aaactggttt aacttcagtt
    27541 gataacttta ttagcacagt tgccttcgca acacttgccc ttttaggttc attatcttta
    27601 ttacttttca aaagaaaaga atctaaataa atcatcgtca cactcataac ttaatatatt
    27661 ttttatttta aattttattt aacctatgtc atagatattt cataatctat aacataggtt
    27721 atttttttat aaaataacgt tgcaattaac taacatttca atgtacaata caagtaatca
    27781 attgataatg attatcagtt gataatatac aattaggagt tgtttctaca acatgaacaa
    27841 acagcaaaaa gaatttaaat cattttattc aattagaaag tcatcactag gcgttgcatc
    27901 tgtagcaatt agtacacttt tattattaat gtcaaatggc gaagcacaag cagcagctga
    27961 agaaacaggt ggtacaaata cagaagcaca accaaaaact gaagcagttg caagtccaac
    28021 aacaacatct gaaaaagctc cagaaactaa accagtagct aatgctgtct cagtatctaa
    28081 taaagaagtt gaggccccta cttctgaaac aaaagaagct aaagaagtta aagaagttaa
    28141 agcccctaag gaaacaaaag aagttaaacc agcagcaaaa gccactaaca atacatatcc
    28201 tattttgaat caggaactta gagaagcgat taaaaaccct gcaataaaag acaaagatca
    28261 tagcgcacca aactctcgtc caattgattt tgaaatgaaa aagaaagatg gaactcaaca
    28321 gttttatcat tatgcaagtt ctgttaaacc tgctagagtt attttcactg attcaaaacc
    28381 agaaattgaa ttaggattac aatcaggtca attttggaga aaatttgaag tttatgaagg
    28441 tgacaaaaag ttgccaatta aattagtatc atacgatact gttaaagatt atgcttacat
    28501 tcgcttctct gtatcaaacg gaacaaaagc tgttaaaatt gttagttcaa cacacttcaa
    28561 taacaaagaa gaaaaatacg attacacatt aatggaattc gcacaaccaa tttataacag
    28621 tgcagataaa ttcaaaactg aagaagatta taaagctgaa aaattattag cgccatataa
    28681 aaaagcgaaa acactagaaa gacaagttta tgaattaaat aaaattcaag ataaacttcc
    28741 tgaaaaatta aaggctgagt acaagaagaa attagaggat acaaagaaag ctttagatga
    28801 gcaagtgaaa tcagctatta ctgaattcca aaatgtacaa ccaacaaatg aaaaaatgac
    28861 tgatttacaa gatacaaaat atgttgttta tgaaagtgtt gagaataacg aatctatgat
    28921 ggatactttt gttaaacacc ctattaaaac aggtatgctt aacggcaaaa aatatatggt
    28981 catggaaact actaatgacg attactggaa agatttcatg gttgaaggtc aacgtgttag
    29041 aactataagc aaagatgcta aaaataatac tagaacaatt attttcccat atgttgaagg
    29101 taaaactcta tatgatgcta tcgttaaagt tcacgtaaaa acgattgatt atgatggaca
    29161 ataccatgtc agaatcgttg ataaagaagc atttacaaaa gccaataccg ataaatctaa
    29221 caaaaaagaa caacaagata actcagctaa gaaggaagct actccagcta cgcctagcaa
    29281 accaacacca tcacctgttg aaaaagaatc acaaaaacaa gacagccaaa aagatgacaa
    29341 taaacaatta ccaagtgttg aaaaagaaaa tgacgcatct agtgagtcag gtaaagacaa
    29401 aacgcctgct acaaaaccaa ctaaaggtga agtagaatca agtagtacaa ctccaactaa
    29461 ggtagtatct acgactcaaa atgttgcaaa accaacaact gcttcatcaa aaacaacaaa
    29521 agatgttgtt caaacttcag caggttctag cgaagcaaaa gatagtgctc cattacaaaa
    29581 agcaaacatt aaaaacacaa atgatggaca cactcaaagc caaaacaata aaaatacaca
    29641 agaaaataaa gcaaaatcat taccacaaac tggtgaagaa tcaaataaag atatgacatt
    29701 accattaatg gcattattag ctttaagtag catcgttgca ttcgtattac ctagaaaacg
    29761 taaaaactaa taaatcgtct ttatatttaa ttattaaatt aacaaatttt aattggcgga
    29821 tgaggtatcc agttacctcg ttcgccaatt atttttcgca atataaaaag tcccacttaa
    29881 aacaatcatt ttaagcggga ctttttatat tgagtaacta aaattattta gctgctactt
    29941 cttcgccatt gtaagaacca cagtttttac atacacggtg tgataatttg tattcgccac
    30001 agtttgggca ttcagtcata cctggtactg aaattttgaa atgcgtacga cgtttgtttt
    30061 ttctagtttt agaagttctt ctttttggta ctgccatgat atatcctcct tagattataa
    30121 acgaaaaata ctaaatgtta gtttaattaa caacattata tcattaatta aactacttat
    30181 tgctctttat catataattg ttgtaatttt tgaagccttg gatcaacttg tcgtgattct
    30241 gaatcatctt gttcttgctg tttagcaagc tcatctaatt gatcctcatc gattacttcc
    30301 caaccattac ctactgtcaa catttggtca ctttgctctg aataagctct cattggtttc
    30361 tcaataataa ctatatcctc gacaatatcc tgaagattaa ccataccatc tttaataatg
    30421 tgatagtgtt catctacatc atcttgatca tcgttatact gattgtaccc ttctaaatca
    30481 aatacttctg tagtagttac atctagtggg acttttactg gtacaagagt acgtgcacaa
    30541 ggcattgtat acgttccagt aatgtgaata tccgcaacga cttctgttga cttaatggtt
    30601 aactgacctt ggattgtaat tggagataaa tcaattaaat ctaatgattc ttttaaattg
    30661 tcaaaactca ccgtttgatc aaattcaaat ggcttacctt gatatttcct taattgcgta
    30721 attgaccatt tcatatggct tcacctctaa caagcacaaa atttatttta actttctaag
    30781 aacaagttgt caagattttt tcttaacata tcatgttctg ttacaatatt aataatgaat
    30841 accgaaagga cgaattgcaa atgaaaagcg ttggcttaat cacagaatat aatccctttc
    30901 ataatgggca tcaatatcat attaatcaat ctaaaaaact tacaaatgct gacgttacta
    30961 ttgcaataat gagtggtaac tttgtcatgc gtggcgaacc agcaatctat aataagttta
    31021 ctcgtgcaaa aatggcatta tcaacagctg atttagttat cgaactacca gcaactgcca
    31081 gtttatcatc tggcgatcat tttgccgaac tagcagttaa agtcgcagat tatatgagtg
    31141 tcgatacaat tgcatttggt agtgaaaata atgatatcaa aacattaaag caattagcac
    31201 acagcattaa tgaaattgaa caatctgaat ccttttcaca aaaagtaaaa gaaggtaaaa
    31261 gctaccctcg tatcattagc gaattattag agcatcatga agcactagca agtcctaaca
    31321 acatacttgg tattagttac ctgaaagcaa ttgctaaaaa tgctaaaaac atcaatgcaa
    31381 tttctatcaa acgagaaaat gctcaacatc atgattcatt aattcaacac catcagtttg
    31441 caagtggtac atctattaga acatcaatca ttagtcaaga tgatcattgg catcatgtgg
    31501 tacctaaaga tattcaacac ttgtacgtaa cacctcatat aacattaaat caaatatttc
    31561 catatttgaa atatcaaatc atagctatga ctacagattc attaaaaaat atttatactg
    31621 ttactgaagg ttttgaaaat cgtttgaaat ctaacattta tgaagcaact gattttcatc
    31681 attttgtaaa attactaaaa acaaagcgtt atacatatac gcatatccaa agactgttaa
    31741 tgaatgtatt attaaacatt aaacctacag atgttacgag taacattcac gcagttaaag
    31801 tattagcgat gaacgaccgt ggcagacaat atttaaagca tttaaaaaca gcgttcccag
    31861 aaagacaata tataaccaat attaacaaaa gcaatgcaca ttacttcaca aatgaaatca
    31921 aagcaacaca catatacaat gcaataagcg ggcaacaaca aacagatttc aacacgcctg
    31981 tcatacagca atatcgttaa caaatgaaaa cagtatttta ggattgtaaa ccatgataaa
    32041 cctaaaatac tgttattttt attacttaaa tttcttcttc aatgcctttt caacataagg
    32101 tggaacgaat tcagaaatat ctgctcgata agctgcaact tctttaacaa tacttgaact
    32161 tataaatgaa taattagtac tagacatcat atataacgtt tcaatttcat tgttcaactt
    32221 tttattcatt gaagttaagc gtaattcata ttcaaaatca ctgactgctc ttaaaccacg
    32281 tatgattgtt ttagctccta cttgttcaca ataatcgact agtaaaccac taaattgatg
    32341 aaccttgaca ttaggtaaat gtttaacaga ttgttcaatt aaatccatac gctcttctaa
    32401 actaaacgta ccttcttttt tactattttt aagaacacag acatgaattt catcaaatct
    32461 atctgtactt ctctcaataa tgtctaaatg accataagta atggggtcaa aactacccgg
    32521 aatgaccgct attgtatgtt ccatgctatt ctcccttttc taataacaat gtgtctgtca
    32581 acccataatg gtaacgttta atcatattaa acggttgata atctatttct tcatgattgc
    32641 taaattcaca aacgatgata ccattttctt tcaataaatt aaactctgaa attagtttta
    32701 aagctttatc aatgagacct ttattataag gtggatctaa gaaaatgaca tcaaattgaa
    32761 tatcacgttt tgacaatgct tttaaagctc tatctgcatt atttttataa acttcagatt
    32821 gtgcctctaa atccaaattc gcaagatttg atttaataac ttttacagct ttaaaatttt
    32881 gatcaacaaa gattacctta tccatacctc gagagagtgc ttctattcca agcgccccgc
    32941 ttcctgcaaa taaatctaaa cctatacctg acacatcata taaactatta aagatacctt
    33001 ctttaacttt atccatagtt ggtctcgtat tacggccttc catactttct aaagctttac
    33061 ttttatgttt acctgcaatg acgcgcatgt tgttcacact tccaattcat ttagttattt
    33121 aatataattt attgagaaaa aggagaatga taaaccaatg aaacaaacat ttattacact
    33181 tggtgaaggt ctaacagatt tgttcgaatt catgacgatg attgaatata accatcaacg
    33241 tattgataaa attatctatt ttcattcacc acaagctgaa aataaaaagt catctgtagc
    33301 aatcattatg aaccctacaa ctggcaatca tttccaagca ttttatatca tgataaacgc
    33361 tattaaatat ccatatccag attcaaataa aaagtttcaa atgataaatg attgtgctga
    33421 aaaattcgac ataccaattt taggtatcga tgtacagccc cctcaagcat ttcatgattt
    33481 atcgttatat tataattatt taattagtgt gttaaggctc caaaaatgga taccagaact
    33541 tcaataatat taattatata tttcgtgttt ctctttttcg taagttttct ttaagtattt
    33601 atatggtgag ccatcaatgt gtctaacgta tttaagttgc attagtttat gtacaatttg
    33661 atcaacatct tgctcattca catacataat tacaaattta cgatctctat ttgaatgaac
    33721 gatatgtcca tattttcgga tttgtcgttc atgtttcata tgttttaaat aaactacaat
    33781 actagttctt gggattaaat tcatttaaac ctcactccat ttattttcag cattaatcat
    33841 atcatgtctt tattaatttt aaaatattaa gctaccttaa ttatgtctta tgtaattgct
    33901 cgataaatgt atttagatta tattaaacat cattatatta attcaattct caagagcatc
    33961 actatatgat taaccacaca tttcgctgta gtcagaacta tgaatttgtt aagctataaa
    34021 agtaaacgag gaggaatttt ttatgaaaaa agtaaacaga aacattttaa atgcggtact
    34081 agtcggtgct ggatttttaa gtagtctatt aatgatcaat aaaaataaag tgattactaa
    34141 aaagcaaacc atccctgctt ttttcaaagg caatgcacca tatatatttg cgcatcgcgg
    34201 tggtatggct ttaagaccag agcaaacgca acttgcattt gactatgcta aacaattagg
    34261 cgttgatggc tttgaaacag atgtgagact cacaaaagat caacagctta tcgtttttca
    34321 tgacgcaact gttgatagaa cgacgaacgg ctctggaaaa gttagtgccc atacattagc
    34381 agaattaaag aaattagacg cagcttatca cttcaaagat atcaatggac taacaccata
    34441 tcgtggtcat gcacacacag ctatactaac cttcgatgag ttgctgaagc aatatcctga
    34501 tatgtatata aacgtagact taaaagatgc tcctgaatcc tatgaaggct caatcgcacc
    34561 acaaattatg tttgatacaa ttgcagaaaa tcaagcattt gaccgcgttc ttgtgacaag
    34621 tttttataaa gaacaaatcg ttcgttttaa taaaattgca caaggatccg tcgcaattgg
    34681 tgctagccaa caggaagtta cagaagcatt tttaaaatat catttattag gtggtaggta
    34741 ctaccaacca ttagcacaaa cgttccaaat gcctactcat tttaaaggca ttgatttgac
    34801 ttcatctcgt tttatcaaat ggttaaatga tatgaatatc attccaggct attatggcgt
    34861 aaatagtata aatctgatga atgatttata ccaaaaaggt gcacacacga ttgtgacaga
    34921 tcgaccagat ttagcacaac aattcaaaca aacaatcccg aataaataaa aacatcgagt
    34981 cagatgctag ttagtggttt caataaccac ctttttctga ctcgatgttt tttataatga
    35041 acaattacag gatccgcccg tcgcacaacc actatgtgat gtttgaaaga atggattacc
    35101 tgtttctatt ttgacatgtt ctgaaacagc gtacgcaatt ttcgtaacaa cttcatctat
    35161 taaatgttga agtgccattt ccctagcttt gtaatgcatc acaacgtcaa gcatctcata
    35221 tgctcttttg cgttgtcttg tctctaacat aactttctta taatcaggat gatatttccc
    35281 gaatcgcatt acttcatcat atttctcttt tgattttaaa aatgattgat aaagtaaatg
    35341 tgcttcgtca ttgttttcga gcgcctgttt agcttgctca aatgatgcat aaatatctga
    35401 tgcaactatc atatcagcta gttcatcaat atcatctaat atcgctagtg aagcttcatt
    35461 aatcataaat acccctcatt gttatttttc taagaaaatt agtgaataat aatctctcat
    35521 tacatctcca cccatctcat cgtattttga atttaataga cgagatcgat gtatatctga
    35581 attcatccaa ctatggatta atgttggtac atcattaaac gcataaccaa cattttgagc
    35641 agttgtttta taagtaacgt gatttttatc taattgccct cttaatgcgt cctctgtaaa
    35701 ttcaacacta tcagaaccat tagaggtcgc ttcatataag ttattagatg caatatgtgc
    35761 taaatcgcta ttgattttca atggttttaa tccttttaat tttctcattt cattcgttac
    35821 ttcataaaga gaaattaatt gatttggatt ttgctcaact ggacgcttat tatgctcttc
    35881 tgacgtagaa ttagaattta attgataagg ttcaatatct gctaacattt cttttgttaa
    35941 aaatcgtaca cttagcacct ttttcgattg ttgatcagaa tacacttgtg catatatgtc
    36001 gccatattta atcagtgttt gtgtttttaa atcttcatct gaaagttcaa attcatattt
    36061 tttaccatca actttaaagg acggttctgg attaatactt gtatgattaa aaatttctgc
    36121 agaatgttgt cctattttta acggactaac attgactttc tcacctgtag catacactga
    36181 aacgatttct tcacgtttag ttgaaacaat gtaataactg tttttgtctt taaacacata
    36241 atttttgtaa ccatctctaa aagggtagac acgatctgct tgtccaaatt tagaagtaag
    36301 cactttaata tctttaccta cccatgtacc aacaccttct ttaggctttg gattttcagc
    36361 atggttattt gatttattca cctgttcaga tctatttact ttattacctt tattcgggtt
    36421 ctctaataca tcaaatttta atctcggtga ataaaaaaga tatattaaaa atccaactaa
    36481 aaataataca ccgacgactc ttataattaa ctttttcatc aatcaaccac ctaaaaagta
    36541 ttaatactat tgtaaaaaac aacacattaa ttagcaaatt ttcaacactg acataactgt
    36601 gtcgtttcga taaaacaaaa tcatcttcag gcataatttt aacgtcataa agattttccc
    36661 tacactctat atcataacct atctttatgt tttcaggttg aatttcatta ttaagattaa
    36721 aatatgtata aaaaaatgga cagttaaggt atcaaatgaa taccatcaac tgtccgacta
    36781 ttcttcatca aaaaacctga taaaacaaat tgccttatca gattagtatc atttgtataa
    36841 gcatattaat gaccaaatgt tgctttaatc agtgatgtcg tttctccacc tggatataat
    36901 acatataata ataagtaaac tgctacacct gtaattgcag tacagaacca tataactgaa
    36961 gcgaatggcc cgaatttgcg gtgtacatta tatttatctt taaatgcagt aataatttga
    37021 actaggccta gaataccacc aattgttgct aaattaatgt ggaaaaataa gaaaatcgta
    37081 taatatttct taattgatgc tggtccgcca aatgctgtat taccgataaa aatcgttcta
    37141 cttgcataga ttaagaaaaa agttaaagca aaaacagctg cagctaacat aacatttttg
    37201 tgtttattta tttccctttt ccaaataagt ctccaaccaa tggcaattaa aattgcacta
    37261 atgacaatac atgtcgtact aatcgttggt aaaattggaa cgcccatatt tttcatccta
    37321 acttaattaa tctagatcaa agtaagtaat gaaacaatca cagctaacac gaaaaagatc
    37381 actaaataat ttagtgaata tataaacatt tgtgttgccc attttgtttg atctgaattt
    37441 ttcttaaatg ttgttaaacc taatgcaatc catcctaaat ttaataatgt agctaacact
    37501 acgaatacga cacctaaatt tattagtaat aaaggtactg gcaataaaat aatcaaccag
    37561 ataaacatac tgacacgtgt acgtttaaag cccttaactg atggtaacat tggaatattt
    37621 gcaagtgcat attcatcttt acgtttaata gctaaggcat aaaaatgaat tggttgccaa
    37681 caaaatacaa ctaaaaacag cgcaatcgct gttaaactaa tttgtccttc aattgcaacc
    37741 catccaatta gtggtggtac tgctccagga aaactcccaa tcactgtgtt ccatgttgta
    37801 tgtcttttag accatattga gtaataagac acataaccta caatccccat aagaccaagt
    37861 acgcctgatg gtatattcaa taaaaacaaa caaatttctc caactaacat cataccaaaa
    37921 cttaatagta ataaattttg atctgtaatt ctattattta cagttggtct attttgttta
    37981 ctaggcataa tacgatcaat atcttggtcg taataattat ttaacgcaca tgcgccaccc
    38041 ataattaaag tagatccaaa tagcattaat aaaatttgag gtattgatga taagaaggaa
    38101 tgatttgtca ttacaactgc tagccatgcg cccgcaaaag ccggaattaa gttaccttga
    38161 acaagtccca ttttaattat ctgttgcaat tctttgaagt taactctgct aatattttgt
    38221 gacaaagtat gctctttgct cataatcccc ctccttaaat ttgttcatat aagattatga
    38281 tatcttagat tgcataaaaa gactaggttt aataaaatta aattgtgaca aattaacgac
    38341 aagagaaaat gtcaattttg tgacacaaa
//
LOCUS       DALAGF010000015        33920 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.015, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000015 DALAGF010000000
VERSION     DALAGF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33920
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.015"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            155..1006
                     /locus_tag="O2692_000697"
     CDS             155..1006
                     /locus_tag="O2692_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCZ4298338.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
     gene            981..1148
                     /locus_tag="O2692_000698"
     CDS             981..1148
                     /locus_tag="O2692_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298339.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            1141..2349
                     /locus_tag="O2692_000699"
     CDS             1141..2349
                     /locus_tag="O2692_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCZ4298340.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
     gene            2830..5352
                     /gene="mprF"
                     /locus_tag="O2692_000700"
     CDS             2830..5352
                     /gene="mprF"
                     /locus_tag="O2692_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCZ4298341.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVAITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGEFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(5555..6064)
                     /gene="msrA"
                     /locus_tag="O2692_000701"
     CDS             complement(5555..6064)
                     /gene="msrA"
                     /locus_tag="O2692_000701"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCZ4298342.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            6201..7184
                     /locus_tag="O2692_000702"
     CDS             6201..7184
                     /locus_tag="O2692_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCZ4298343.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            complement(7272..7457)
                     /locus_tag="O2692_000703"
     CDS             complement(7272..7457)
                     /locus_tag="O2692_000703"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCZ4298344.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            7605..8867
                     /locus_tag="O2692_000704"
     CDS             7605..8867
                     /locus_tag="O2692_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCZ4298345.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            complement(9004..10095)
                     /locus_tag="O2692_000705"
     CDS             complement(9004..10095)
                     /locus_tag="O2692_000705"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCZ4298346.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            10260..11291
                     /locus_tag="O2692_000706"
     CDS             10260..11291
                     /locus_tag="O2692_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCZ4298347.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGN
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            11783..13189
                     /locus_tag="O2692_000707"
     CDS             11783..13189
                     /locus_tag="O2692_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCZ4298348.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVIKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITELMVIEKYEHVMHIVSEVTGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            13186..13752
                     /locus_tag="O2692_000708"
     CDS             13186..13752
                     /locus_tag="O2692_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCZ4298349.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTKDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            13758..14756
                     /gene="trpD"
                     /locus_tag="O2692_000709"
     CDS             13758..14756
                     /gene="trpD"
                     /locus_tag="O2692_000709"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HCZ4298350.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALEKYHQMRGE"
     gene            14758..15540
                     /gene="trpC"
                     /locus_tag="O2692_000710"
     CDS             14758..15540
                     /gene="trpC"
                     /locus_tag="O2692_000710"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HCZ4298351.1"
                     /translation="MTILSEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKTNHYYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            15540..16172
                     /locus_tag="O2692_000711"
     CDS             15540..16172
                     /locus_tag="O2692_000711"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HCZ4298352.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            16165..17379
                     /gene="trpB"
                     /locus_tag="O2692_000712"
     CDS             16165..17379
                     /gene="trpB"
                     /locus_tag="O2692_000712"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCZ4298353.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMVERGLAND"
     gene            17372..18100
                     /locus_tag="O2692_000713"
     CDS             17372..18100
                     /locus_tag="O2692_000713"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCZ4298354.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIKYLQSIQQTLNN"
     gene            18422..19684
                     /gene="femA"
                     /locus_tag="O2692_000714"
     CDS             18422..19684
                     /gene="femA"
                     /locus_tag="O2692_000714"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HCZ4298355.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            19703..20962
                     /gene="femB"
                     /locus_tag="O2692_000715"
     CDS             19703..20962
                     /gene="femB"
                     /locus_tag="O2692_000715"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HCZ4298356.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
     gene            21651..22418
                     /locus_tag="O2692_000716"
     CDS             21651..22418
                     /locus_tag="O2692_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCZ4298357.1"
                     /translation="MRFVFDIDGTLCFDGRLIDQTIIDTLLQLQHDGHELIFASARPI
                     RDLLPVLPSVFHQHTLIGANGAMISQQSKISVIKPIHTDTYHHIFKIIQKYELDYIID
                     DDWNYAAQLDAENAIFERLDPHKLASCIDVANIDTPIKIILLNIDPAQITTILDELDK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIVMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHINNNAQAICKVLKSYK"
     gene            complement(22466..22939)
                     /locus_tag="O2692_000717"
     CDS             complement(22466..22939)
                     /locus_tag="O2692_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCZ4298358.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSICNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDY
                     QLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
     gene            complement(23063..23764)
                     /locus_tag="O2692_000718"
     CDS             complement(23063..23764)
                     /locus_tag="O2692_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298359.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQAFSY"
     gene            complement(23757..24530)
                     /locus_tag="O2692_000719"
     CDS             complement(23757..24530)
                     /locus_tag="O2692_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298360.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENVSRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKGSKEEALSKVDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            complement(24517..25347)
                     /locus_tag="O2692_000720"
     CDS             complement(24517..25347)
                     /locus_tag="O2692_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4298361.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITLIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            complement(25340..26326)
                     /locus_tag="O2692_000721"
     CDS             complement(25340..26326)
                     /locus_tag="O2692_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4298362.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKIKTNTPLISESSDRHA"
     gene            complement(26497..26682)
                     /locus_tag="O2692_000722"
     CDS             complement(26497..26682)
                     /locus_tag="O2692_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298363.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SAIVVFVLTFLYFTWLT"
     gene            complement(26630..26974)
                     /locus_tag="O2692_000723"
     CDS             complement(26630..26974)
                     /locus_tag="O2692_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298364.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYG
                     YEGAELRRKDKK"
     gene            27179..28993
                     /gene="pepF"
                     /locus_tag="O2692_000724"
     CDS             27179..28993
                     /gene="pepF"
                     /locus_tag="O2692_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCZ4298365.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
     gene            complement(29133..29774)
                     /gene="phoU"
                     /locus_tag="O2692_000725"
     CDS             complement(29133..29774)
                     /gene="phoU"
                     /locus_tag="O2692_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCZ4298366.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(29781..30632)
                     /locus_tag="O2692_000726"
     CDS             complement(29781..30632)
                     /locus_tag="O2692_000726"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298367.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            complement(30679..31596)
                     /gene="pstA"
                     /locus_tag="O2692_000727"
     CDS             complement(30679..31596)
                     /gene="pstA"
                     /locus_tag="O2692_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCZ4298368.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            complement(31598..32524)
                     /gene="pstC"
                     /locus_tag="O2692_000728"
     CDS             complement(31598..32524)
                     /gene="pstC"
                     /locus_tag="O2692_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCZ4298369.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            complement(32715..33698)
                     /locus_tag="O2692_000729"
     CDS             complement(32715..33698)
                     /locus_tag="O2692_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCZ4298370.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
BASE COUNT        11796 a         5124 c         5687 g        11313 t
ORIGIN      
        1 attatagtta ttccactttg agagacatcg aaattaaatt aagatatatc atataggata
       61 ggcgtgtaat agaacacaag tatttaaatt attatacttt caagttagac atttccgata
      121 tataatctta ataatagaaa aataggtgat gataatggga gaatatattg ttactaaaac
      181 attgaacaac aatgtcgtag tatgtactaa taatgatcaa gaagttattt taatcggtaa
      241 aggtattggt tttaacaaaa aagagggaat ggcgttaaac gaccaaacta ttacaataga
      301 gaaaatttat aaattagaaa gtgagcaaca aaaagcacat tataaaagtt tagttgaaat
      361 cgctgatgat aatgtattac aagtaattat tgattcgttg aattttattt ctaatactgc
      421 gatgaatgtt gattcaaaac aacttgtagt ttcattaacg gatcatatta tatttgctta
      481 taaacgctta aaacaaaatc aagttattag caatccattt gttatggaaa ctatgcagtt
      541 atatagtgat gcatatcata ttgctaaaca ggtgattgat cagttaaatg cagcattaga
      601 tgtacatttt cctgaagatg agataggatt tattgcatta catattgcat ctaatacaga
      661 agatttatct atgcatgaga tgaccttgat caataatgtt attaaaaaag gtatagatat
      721 cattgaatca gaccttgtga caactgttga taaggaatca ttacaatacc aacgttttat
      781 aaggcacgta caatttttaa ttcgccgatt aagaagaaaa gaatatatac atgcacaaga
      841 tgattttgtg tctatgatta aaaatcacta tccgatttgc tataacacag catataaaat
      901 tttaactatg atacaaaaac aatttgatgt taatatcagt gagtctgaaa ttatatattt
      961 aacattacac attcatcatt ttgaagaaag gattaatcaa tcctaaatta agtgtattac
     1021 acgggcgttt cgttactgta aagggtattt gtatggtatc attgatgtta gtaattttac
     1081 ataagtggac tgtttttata caacatctta tgaatatttt aagtggtggt gaatctataa
     1141 ttgaatgaaa atgaaaagaa tataagaaag aattttttaa aatttaccga atcacggtat
     1201 atgaagtttg ttggtgggaa tgatttagtc ttctcattaa tagcgctagt attgttgggt
     1261 attgttattt ttattttcga aaaagtatca tatgtttttg atccttttat catcgttttt
     1321 aagacgatag cagcacctat catcgtctct ttaattctat tctatctatt taacccaatc
     1381 gtaaatatga tggaacgtta tagaatacca agagttgcag gtatttctat tatttatcta
     1441 gctgtagtag gtgttattac gttaattgtt aatttattga tacctattat tggttcgcaa
     1501 gtagatagtt tagttaaaaa ttcaccgcaa tatctagaaa aattaattaa ttctattgat
     1561 aaaatagcaa ataatacgtt tttctcttcg tattatagtc aaattaatga ttggttaaat
     1621 tctttaccta agaaaatacc atctatgtta agtgaattta cagatggctt tgggtctaaa
     1681 attgcaacgt ttgcagaaac gattgctaat attggcgttg tgattgtcac aacaccattt
     1741 gtactattct ttatgcttaa agatggacat cacttcaaag aattttcaac gaatattatg
     1801 ccaccgaaat tccgaaaaga ttttcatgat ctacttgaaa aaatgagtgt tcaagttggt
     1861 tcatacattc aaggacaaat tatcgtttca ttctgtatcg gtatactgtt gtttatcggt
     1921 tattcggtta tcgggttgaa atatagctta gtattagcta gtattgcggc agttacaagt
     1981 gttgtaccat atttagggcc tactatagcg atttctccag ctattgtaat agctgctata
     2041 acatcgccgt ggatgctctt aaaattagca gtagtatgga ctttagtaca atttgttgaa
     2101 gggcacttca tttcaccaaa tatcatgggt aaaacactta agattcatcc acttacaatc
     2161 attttcattt tactgtgtgc aggcaaattg cttggtattg taggcgttat tttaggtatt
     2221 ccgggatatg ctattttaaa agtattagtt actcatttat tccaattatt taaacgtcga
     2281 tacaatcgtt tctatggtaa tgatgtaggt gaatatgata ttaaagaaag taataaaata
     2341 gttgaataag taataaaaaa taccaatgac tatatgtttg ttggtatttt ttatggtgct
     2401 tataatgttg ggcagttaca tttatgatta tcagcacatg tttagcttat aaatttttac
     2461 gaaagtttag ccgtttataa agcacatgca taatgaaacg agtatttgcc acttgattag
     2521 tacttcatta ttatgtcgaa aataaaaata agtggtattt ttaatatatt aagaagcact
     2581 cataatcggc tgttaattaa taatattttt cataagtatt gattcatcat tttctttatg
     2641 ttaaatatga atcagtatag ataaccatat tgttctgttt gagatagaaa tattttctat
     2701 ttacttctta atacagatat caatatgaca aaagtgttat ataaatcaaa ggtaaatgat
     2761 atgctatttt tatggtttat atgatatatt ttgatttata acagaaataa ttagaattga
     2821 tgtgaaaaaa tgaatcagga agttaaaaac aaaatatttt caatcttaaa aattacgttt
     2881 gctacagctt tatttatttt tgtagcaatc acattgtatc gggagttatc tggtattaac
     2941 tttaaagata cgttggttga atttagtaag attaaccgta tgtccttagt gttactattt
     3001 attggtggtg gggcatcgct tgttattcta tcaatgtatg atgtgatttt atctagagct
     3061 ttaaaaatgg atatatcctt aggcaaagtt ttaagagtaa gttatatcat caatgcattg
     3121 aatgcgattg taggtttcgg tggctttatt ggtgcaggcg ttagagcaat ggtttataaa
     3181 aactatacgc atgataaaaa gaaattagtt cactttatat ccttaatact tatttcaatg
     3241 ttgacaggtt taagcttatt atcattgcta attgtattcc atgttttcga tgcatcttta
     3301 atcttagata agattacatg ggtaagatgg gtattatatg tagtgtcatt tttcttacca
     3361 ttattcatta tttattcaat ggttagacca cccgataaaa acaatcgttt tgtaggattg
     3421 tactgcactt tagtgtcgtg tgttgaatgg ttagcagctg cagttgtatt atatttctgt
     3481 ggtgtaattg ttgacgctca tgtatcattc atgtccttta ttgcaatatt tatcattgct
     3541 gcattatcag gtttagtcag ctttattcct ggtggtttcg gcgctttcga tttagttgta
     3601 ttactaggat ttaaaacttt aggtgtccct gaggaaaaag tattattaat gctacttcta
     3661 tatcgttttg cgtactattt tgtaccggta attattgcat taattttatc atcatttgaa
     3721 tttggtacat cagctaagaa gtacattgag ggatctaaat actttattcc tgctaaagat
     3781 gttacgtcat ttttaatgtc ttatcaaaag gatattattg ctaaaattcc atcattatca
     3841 ttagcaattt tagtattctt tacaagtatg atcttttttg taaataactt aacgattgtt
     3901 tacgatgctt tatatgatgg aaatcactta acgtattata ttctattggc aattcatact
     3961 agtgcttgtt tattactttt actgaatgta gttggtattt ataagcaaag tagacgtgcc
     4021 attatctttg ctatgatttc aattttatta atcacagtgg cgacattctt cacttacgct
     4081 tcatatattt taataacatg gttagctatt atttttgttc tgcttattgt agctttccgt
     4141 agagcacgta ggttgaaacg cccagtaaga atgagaaata tagttgcaat gcttttattc
     4201 agtttattta ttttatatgt taaccatata tttattgctg gaacgttata tgcattagat
     4261 atttatacga ttgaaatgca tacatctgta ttgcgctatt acttctggct tacgatttta
     4321 atcatcgcta tcatcatagg tatgattgca tggttgtttg attatcaatt tagcaaagta
     4381 cgtatttctt ctaaaattga agattgcgag gagattatta atcagtacgg cggtaattat
     4441 ttgagtcact tgatatatag tggtgacaag cagtttttca ctaatgaaaa taaaacagca
     4501 tttttaatgt atcgttataa agcaagttca ttagtggttc ttggagatcc gttaggtgat
     4561 gaaaatgcct ttgatgaatt gttagaagca ttctataatt acgctgagta tttaggctat
     4621 gatgttatat tctatcaagt tacagatcaa cacatgcctt tatatcataa tttcggtaac
     4681 caatttttca aattaggtga agaagcaatt attgatttaa cgcaattttc aacttcaggt
     4741 aaaaaacgcc gtggatttag agcgacttta aataaattcg atgaacttaa tatttcgttc
     4801 gaaattattg aaccaccgtt ttcaactgaa tttataaatg aacttcaaca tgtaagtgat
     4861 ttatggctag ataatcgtca ggaaatgcat ttctctgttg gtgaatttaa tgaagaatac
     4921 ttatctaaag cgccaattgg tgtaatgcga aatgaagaaa atgaagtaat tgcattttgt
     4981 agtttaatgc caacatactt taatgatgcc atttcagtcg atttaattag atggttgcca
     5041 gagttagatt taccattaat ggatggtcta tacttgcata tgttactttg gagtaaagaa
     5101 caaggttata caaaatttaa tatgggtatg gcaacgttat cgaacgttgg tcaattgcat
     5161 tattcatatt taagagaacg acttgcaggc cgtgtctttg aacatttcaa cggtctatat
     5221 cgtttccaag gattacgtcg ttataaatct aaatataatc cgaattggga accacgcttt
     5281 ttagtttatc gtaaagataa ttcgctttgg gaatcacttt ctaaagtaat gcgtgtaata
     5341 cgtcacaaat aattaaaatc caagtgctaa gaggtataca gttatgcctg atgcgcttgg
     5401 attttttggt ttctataaag aatgatttat aaaaaacggt acagataaga atatttaagt
     5461 ttcctatctg taccgtattt taatattgaa atgagctaaa gttaagtcgc ctaactttta
     5521 ttatcagtcc aatcagtttc atcaagtata aatgttattg cttatttttg tattcttggc
     5581 gtattttttg ttcttctgca tagcgctctg gatttttctt ataaaaatct tggtgatagt
     5641 cttcggcttt gtaaaattgt gacgctggta atatttttgt tgcaattgcc ttatcagcat
     5701 taatcgtatt tttaagctgc tcgatataag tctcagcgag ttctttttga tgatcattag
     5761 tgtagaaaat agctgtttga tattgaggac cacggtcttg atattgacca cctgtatcta
     5821 atgggtcaat gactgagaaa aatatttcta ataacttatt gtatgagaat aatgcaacat
     5881 catattgaat ttcaacagtt tctaaatgac cactcgtacc tgattttact tgttcgtaag
     5941 taggattttc aatatgtccg cccatatatc cagaagttac tttttctatg ccgtcaaagg
     6001 tgtcaaatgg tttcgtcata caccaaaagc aacctccggc aaaataagct gtattaatat
     6061 tcatttttga catcctttca ttagacctta gtacgattta ttaagaaatc acttgctttt
     6121 tgaattgttt ttatataacg ttaatatgtg attattatag tacacaaaaa tctatttttg
     6181 aagatataag gtaggtaaat atggataaag aaactaatga caacgaatat agacgtcaaa
     6241 gtgaacatcg cacttcggcg cctaagcgaa aaaagaagaa gaaaattagg aaattaccta
     6301 tcattcttct gattgttgta attttactta tcgcattagt tgtatatatt gtacatagtt
     6361 acaatagcgg tgtagaatat gccaagaaac atgcgaaaga tgttaaagta catcaattta
     6421 atggaccagt aaaaaatgat ggtaaaattt ctattcttgt actcggtgca gataaagcac
     6481 aaggtggaca atcaagaaca gattctatca tggttgttca atatgacttt atcaataaaa
     6541 agatgaaaat gatgtctgtc atgcgtgata tttatgcaga tattccagga tatggaaaac
     6601 acaaaattaa ttcagcatac gctttaggtg gtccagagct acttagaaaa acacttgata
     6661 aaaatttagg aattaatcct gaatattatg cagtagttga ttttactgga tttgagaaaa
     6721 tgattgatga attaatgcct gaaggtgtac caattaatgt cgaaaaagat atgtcgaaaa
     6781 atattggtgt atctttgaaa aagggtaacc ataggttgaa tggtaaagaa ttacttggtt
     6841 atgcaagatt ccgtcacgac cctgaaggtg acttcggacg cgtgcgacgt cagcaacaag
     6901 tgatgcaaac attgaaaaaa gaaatggtta attttagaac agttgttaaa ttaccaaaag
     6961 ttgcaggtat tttaagaggc tatgtgaata caaacattcc tgattcaggg attttccaaa
     7021 caggtttgag ttttggtatc cgaggtgaaa aagatgttaa gtcattgact gtgccaatca
     7081 agaactcata cgaagatgtc aatacaaata ctgatggtag tgcattacag attaataaaa
     7141 acacaaataa acaagctatt aaagactttt tagatgaaga ttaaaaataa acaaggcgat
     7201 ttctatcata ctcagataga agtcgccttg ttcgttttca aagaattaat tggcatgcat
     7261 cttatgaaga attattgatc tgactttctt acgccagcca caccataatg gtttggtttc
     7321 atttcttcta taacaacgtg aattgcttgt ctatttgccc ccgttgtttt ttctacggcg
     7381 tcagttactt cgctaactaa attttttaat tgttcatccg aacgaccttc taataatttt
     7441 acattgacga ttggcatcat ccaaacctcc taatcattat taatttgagt atatcatgta
     7501 atacttaaaa attcatttgc aaaataacag aacatttgtt ctaaaatagt tgaaatagaa
     7561 cacgtgttcg tatataataa gattcaagta agggaggaat aacagtgtat aattatcatt
     7621 tattagaaga tagggatgtt ctatgtattg accaaaaaag tttttttgcg agtgtttctt
     7681 gtattgaaaa ggggctagat ccattagaaa caaagctagc tgttgttgca gatactaagc
     7741 gtcagggttc tgtaatattg gctgcgacac ctaaattaaa agaattaggc atcaagacag
     7801 ggtcgcgatt gtttgaaata ccacatagaa atgatattta cattatcaat ccaagtatgc
     7861 gtaaatatct taatgtttca gttgctattt ctaagattgc attgcgttat attccacctg
     7921 aagatttaca ccaatatagt attgacgaat tttttatgga tgttactgat agctatcata
     7981 gatttagttc tacagtacat gcattttgcg aaagacttaa acgtgaaatt tatgaagaaa
     8041 caggcattta ttgtactgtg ggcattggtt ctaatatgtt attaagtaaa attgctatgg
     8101 atgttgaagc gaagcatagt caaaatggta tagctgaatg gcgatatcaa gatgtaccaa
     8161 cgaaattatg gccaattcag cccttgcgag atttttgggg tattaatcgt cgaacagaag
     8221 ccaaattgaa taaaagagga atttttacta taggagattt agcgaaatat ccatataaat
     8281 ttttaaaaaa agagttcggt attttaggtg ttgatatgca tctacatgcg aatgggatag
     8341 atcagagtaa agtacgtgaa aagcacaaga tcagcaatcc atcgatatgc aaaagtcaaa
     8401 tattaatgag agattatcat tttgatgaag caaaagtagt aatgcaagag ttaattgaag
     8461 atgttgctag cagagttcga gcaagaaaaa aagtggcaag aacgatacat tttgcctttg
     8521 gctatagtga tgaaggcggt gtacataagc aatatacttt gaaagatcca acaaacttag
     8581 aaaaagatat ttataaagta gtaatgcatt tcgcagataa attatgtaat aaacaagcac
     8641 tatatcgtac gctaagtata tctttgagtc aatttattaa tgaggatgag cgacagttaa
     8701 gtctgtttga agatgaatac caacgcaaac gtgacgaatg tctagctaaa acgatagacc
     8761 aattacattt gaaatacggc aaaggcattg tgtccaaagc agtatcgttt acagaagcag
     8821 gtacaaaaca cggcagatta ggtttaatgg ctggacataa aatgtaatga ctatacggtt
     8881 taagtaatat ataactgtga ttcgtataaa ataagtctct aaagataaat atttcatata
     8941 tcacaataga ttttcacaat aatatctaag aatacatgga atttatcaaa agagacttaa
     9001 taattattgg atataacaat caaaatcact caatgcttgc ataccgcgtt ctcggtcagt
     9061 agggtttttg aaactaattt ttaaagcacc gtatatatct tcgcgtactt ctaagattct
     9121 taagttgctt atagatatgt tatgtaaact caggatataa gtcactttac ttatcatacc
     9181 tgattcatcc ggaatgtcta catatagatc atacgcagta tttagtccac ctagttgttt
     9241 agcgggtagt gcgtcgcgat acgatttagc ttgggcaaaa aatgataaca atttttcaga
     9301 atcattgctt tcaattagtc tttctaaatc ttgaaactga ctttttagct gtcgaatcat
     9361 ttctaaaata tacgttttat tactcaaggt gatatctttc cacatttgtg cattactact
     9421 agctatacga gtgatatcac gaaaaccacc agctgcaagt ttattaacta aatgatgttc
     9481 ttgaccgttc ttttgactaa catgaactaa actagatgca acgatatgag gtaaatgact
     9541 tacgacgctt gttacgtagt cgtgttcttc agcagtagtt acaataaatt tagcaagagt
     9601 aggtgataac agttctttta acgtgtttgc tgcttgctca tttcttggct cattgtagac
     9661 taaaatataa taagcgtttt caaataagtg ctttttagca tttagtacac cagatttatg
     9721 actaccagcc attggatgac cactgactaa atgaatatta tgctttaata aattgcattc
     9781 gtgttgctgt atcattgctt tagtactacc agtatcagaa acaataacac caggtttagt
     9841 tggcatatct ataagctcgc taagatattt atttgtgata gcaacaggtg ttgcataaat
     9901 aattacatcg gcttttttaa tagcttcact ataatttaaa catttttcat taataatgcc
     9961 gattgattta gctttatcta actgagaagt atctgcatcg tatgcaataa tattagtatt
    10021 agggttatgg tattttatat tgctagcaag acttccacca attaatccaa gcccaacaaa
    10081 taaaactgtt gtcatataaa tcaccttatt tcgaaatttt cagaataata acattgtaaa
    10141 tgagctgttg acacagtgca atagtaaata aaaatcgata atagcattaa tagataaacg
    10201 gagataaatc atctacaata aagagtatag taacacaatg gcaacggagg ggtaaatcaa
    10261 tggaaccaat attagaaatg attaaaacat taacaggtat taatagtcct tcaggagaca
    10321 cagaagaagc aattcaattt gtcgaaaaat atgcaaaaga cttgggttat caaacaacac
    10381 taacaaataa aggtgcgtta ttaataacag tgccaggcaa aaatgatgaa gtacaacgct
    10441 gtattactgc tcatgttgat actttaggtg caatggttaa agaaattaaa gaagatggtc
    10501 gcttagcaat agaattaatt ggaggattca cgtataacgc gattgagggt gaatattgcc
    10561 aaattaaaac tgatgctggt caaatatata caggaacaat ttgtctgcat gaaacaagtg
    10621 ttcatgtata tagaaataat catgaaatac ctagagatca aaagcatatg gaaataagaa
    10681 ttgatgaagt aactacatca gaagaagata caaagagttt aggtatttca gtaggtaatt
    10741 ttgttagctt tgatccacgt acagttatca cgtcatcagg ttttattaaa tctcgtcatt
    10801 tagatgataa agctagcgta gcgatgatac tacaattact aaagaaatta aaagaagagc
    10861 aaataatatt accacataca acgcaatttt atatttctaa taacgaagaa ataggttacg
    10921 gtgcaaatgc atcaattgat tcgaaaatca aagaatatat tgcattagat atgggcgcgt
    10981 tgggagacgg tcaagcatcg gatgaatata cagtttctat ttgtgccaaa gatgcttcag
    11041 gtccatatca taagcaattg aaatcgcacc tagttaatct ttgcaaaata aataacattc
    11101 catataaagt agacatatat ccatattatg gttcagatgc ttcagcagct ttacatgctg
    11161 gtgcggatat cagacatggt ttatttggcg ctggcattga atcatctcat gcaatggaac
    11221 gaacacatat tgattctatt aaagcgacag agaaattact atatgcatat tgcttatcac
    11281 caattgagta aacaattagt gttgacaaat gtgaacgacc tatgtaatat aatgaactat
    11341 aaaaataatt agaattttct aaagaaatag tagcagatat gaaacgtagc aaatagaaag
    11401 ctaatgggtg atgggaatta gcacgccata tcttgtgaat tggactttgg aaaacaattg
    11461 aatgagtttt gaaagtgaac atgaattatg ttaactaagg tggcaccacg gtaacgcgtc
    11521 cttacaggta tatgcgttat gtggtgtctt tttatttaga caaaatgtag tagttaatta
    11581 aaggtagcaa cagaaagtta gtggatgatg tgaactaaca ccgagattaa tgaaattggg
    11641 ttttgtctgc aacagaaaaa ttatatatag taaagagtga actatgaata tttcgaatat
    11701 tcggttaatt taggtggtac cacgcgtcag cgtcctttat attgataagg atgctggcgc
    11761 ttttttgaaa ggagcgtata gaatggatat attttataaa aaaataaaag caaatgtaac
    11821 gcccgaagtt ttagcacaac ttcattccaa gaagatcatt ttggaaagta caaatcaaca
    11881 acaaactaaa ggtcgctatt cagttgttat ttttgatatt tatggcactt taactttaga
    11941 taatgatgta ttatcagtaa gtactttaaa agaatcgtat caaatcactg aaagaccgta
    12001 ccattattta acgactaaaa taaatgaaga ctaccataat attcaagatg agcaacttaa
    12061 gtcattacca tttatatctg gatatgttgg gacgtgtagc tttgatttag taagacatga
    12121 atttcctaaa ttgcaatcaa tacaattaga agatcacaag cagcacgatg taaggttata
    12181 tatggttgaa caagtttatg tatttgacca ttacaaagat gagttatata tcatcgcgac
    12241 gaatcaattt tcaaattcaa caaaatcaga tcttgagaat cgagttatta agtctatcga
    12301 agacttaact aaaatccaac cattcatgcc tacacaagat tttgatttta aaactaaaga
    12361 aattcaatca aacatttctg aagaaagatt tatcgaaatg attcagtatt tcaaagagaa
    12421 aataacagaa ggggatatgt tccaagttgt gccatcaaga atttacaaat atgcgcatca
    12481 tgctagtcag catttaaatc aactttcgtt tcaactgtat caaaatttaa aacgacaaaa
    12541 cccaagtcca tatatgtatt atcttaatat cgatcaacca tatattgtcg gtagttctcc
    12601 cgaaagtttc gtaagtgtca aagatcaaat tgtaacaact aatcctattg caggtacgat
    12661 tcaacgtggt gagacgacac aaatagataa tgagaatatg aaacaactac ttaatgatcc
    12721 aaaagaatgc agcgaacatc gtatgctagt tgatttagga cgtaatgata ttcatagagt
    12781 aagtaaaatc ggtacctcaa aaattactga attaatggtt attgaaaaat atgaacatgt
    12841 tatgcatatc gtaagtgaag tcacaggtaa aataaatcaa aatttatcgc caatgacagt
    12901 tattgcgaat ttattaccaa caggtaccgt ttcaggtgca ccaaaattac gtgcaattga
    12961 aagaatatat gaacaatatc cacataaacg gggcgtttat agtggtggtg ttggatacat
    13021 aaattgtaat cataacttag attttgcatt agcaattcga acgatgatga tagatgagca
    13081 gtatatcaac gtagaagctg gttgtggcgt tgtatatgat tctattcctg aaaaagaact
    13141 gaatgaaacg aaattgaaag ctaaaagctt attggaggtg agcccatgat cttagttgta
    13201 gataattatg attcctttac atataaccta gtggatattg ttgctcaaca tactgacgtc
    13261 attgttcaat accctgatga tgataatgtg ctgaatcaat cggtggacgc tgttattata
    13321 tctcctggtc cagggcatcc attagacgat caacagttaa tgaaaatcat atcaacctat
    13381 caacacaaac ccattttagg tatttgttta ggggctcagg cactgacttg ttactacggt
    13441 ggagaagtca ttaaaggcga caaggttatg cacggcaaag ttgatacact aaaggttata
    13501 tcgcatcatc aacatctgtt atatcaagat ataccagaac agttttcaat tatgagatat
    13561 cattcattaa taagtaaccc tgacaatttt ccagaagaat tgaaaattac tggacgtacc
    13621 aaagattgta tacagtcatt cgagcataaa gaaagaccgc attatggtat tcagtaccat
    13681 cctgaatcat ttgctacaga ctatggtgtc aaaataatta caaatttcat taatctagtg
    13741 aaggaaggat gaaaaccatg acattactaa caagaataaa aactgaaact atattacttg
    13801 aaagcgacat taaagagcta atcgatatac ttatttctcc tagtattgga actgatatta
    13861 aatatgaatt acttagttcc tattcggagc gagaaatcca acaacaagaa ttaacatata
    13921 ttgtacgtag cttaattaat acaatgtatc cacatcaacc atgttatgaa ggggctatgt
    13981 gtgtgtgcgg cacaggtggt gacaagtcaa atagtttcaa catttcaacg actgttgctt
    14041 ttgttgtagc aagtgctggc gtaaaagtta taaaacatgg taataaaagt attacctcaa
    14101 attcaggtag tacggatttg ttaaatcaaa tgaacataca aacaacaact gttgatgata
    14161 cacctaacca attaaatgaa aaagaccttg tattcattgg tgcaactgaa tcatatccaa
    14221 tcatgaagta tatgcaacca gttagaaaaa tgattggaaa gcctacaata ttaaaccttg
    14281 tgggtccatt aattaatcca tatcacttaa cgtatcaaat ggtaggcgtc tttgatccta
    14341 caaagttaaa gttagttgct aaaacgatta aagatttagg tagaaaacgt gcaatcgttt
    14401 tacatggtgc aaatggtatg gatgaagcaa cactatctgg tgataatttg atatatgaat
    14461 tgactgaaga tggagaaatc aaaaattaca cattaaatgc gactgattat ggtttgaaac
    14521 atgcgccgaa tagtgatttt aaaggcggtt cacctgaaga aaatttagca atctccctta
    14581 atatcttgaa tggtaaagat cagtcaagtc gacgtgatgt tgtcttacta aatgcgggtt
    14641 taagccttta tgttgcagag aaagtggata ccatcgcaga aggcatagaa cttgcaacta
    14701 cattgattga taatggtgaa gcattggaaa aataccatca aatgagaggt gaataatatg
    14761 acgattttat cagaaattgt taaatataaa cagtcacttt tacaaaatgg ctattatcaa
    14821 gacaaactta ataccttgaa aagtgtgaag attcagaata aaaaatcttt tataaacgca
    14881 attgagaaag aaccaaagct agcaattatt gcagaaatta aatcgaagag tcctacagtt
    14941 aatgacttac ctgaacgaga tttatcgcaa caaatctcag attatgacca atatggtgca
    15001 aatgccgtgt ccattttaac tgatgaaaag tactttggtg gtagttttga aagattacaa
    15061 gcattgacga caaaaacaac attacccgta ttatgcaaag actttattat agacccgctt
    15121 caaattgatg ttgctaaaca agctggtgca tctatgattt tattgatcgt taacatctta
    15181 tctgataaac aattgaaaga tttatataac tacgctatat cgcaaaatct agaagtgtta
    15241 gttgaagtac atgatcgcca tgaattagaa cgtgcctata aggttaatgc taaattgatt
    15301 ggtgtaaata acagggactt aaaacgattt gttacaaatg tggaacatac aaatactatt
    15361 ttagaaaata aaaaaacaaa tcattattat atttctgaaa gtggtattca cgatgcatct
    15421 gatgtaagaa aaatcttgca tagtggtatc gatggcttac taataggtga ggcgcttatg
    15481 cgttgtgaca atctatctga atttttacca caactgaaaa tgcaaaaggt gaagtcatga
    15541 tgaaattgaa attttgtggc tttacatcaa taaaggatgt tacagcggcc agtcaattac
    15601 ctattgatgc gataggtttc atccattatg aaaaaagtaa aaggcatcaa acaattaccc
    15661 aaataaaaaa gttagcgtct gctgttccaa atcatatcga taaagtatgt gtcatggtaa
    15721 atcctgattt aacaacaatt gaacacgtat taagcaatac gtcaattaac acaatacagt
    15781 tacacggcac agaatctatt gattttatac aggaaattaa aaagaaatat tcaagcatta
    15841 aaatcactaa agctttagct gcagatgaaa acataatcca aaacataaat aaatataaag
    15901 ggttcgtaga tttatttatt atcgacacac cctcagtgtc gtatggtggt accggtcaaa
    15961 catatgactg gactattttg aagcacataa aagacatacc ttatttgata gcaggaggca
    16021 ttaactctga aaatattcaa acagttaatc aacttaaatt atcacatcaa ggttatgatc
    16081 ttgcatcagg tatagaagta aatgggcgaa aagatataga aaaaatgaca gcaattgtaa
    16141 atattgtgaa aggagataga gacaatgaat aaacaaatac aaacagaagc agatgaatta
    16201 ggtttctttg gtgaatatgg agggcaatat gttccagaaa cattaatgcc agcaattatt
    16261 gagttgaaaa aagcttataa agaggcaaaa gcagacccag agtttcaaag agaactggaa
    16321 tactatttat cagagtatgt aggacgcgcg acaccactta catatgctgc atcatatact
    16381 gaaagcctag gtggcgctaa aatatatttg aaacgagagg atctaaatca tacaggcgcc
    16441 cataaaatta ataatgcgtt aggtcaagcg ttgcttgcta aaagaatggg caagaagaag
    16501 cttgttgctg aaactggtgc gggtcaacat ggtgtagcta gtgctacggt tgctgcatta
    16561 tttgatatgg aacttgttgt ctttatggga agtgaagata ttaaaagaca acaacttaat
    16621 gtatttagaa tggaattact tggtgcaaag gttgtggcag ttgaagatgg tcaagggact
    16681 ttatcggatg cagttaataa agcattgcaa tattgggtaa gtcatgtaga tgatacacat
    16741 tatttattag gttctgcatt aggtccagac ccgttcccaa cgattgttag agattttcag
    16801 agtgtgattg gtaaagaaat aaaatcacag atattgaaga aagaaggtcg acttccggat
    16861 gcaattgtag catgtatcgg tggtggctca aatgcaatcg gtacatttta tccatttatt
    16921 aaagatgatg ttgcattata cggtgttgaa gccgcaggtc aaggcgatga tactgataaa
    16981 catgcacttg caattggcaa aggatcacct ggcgtattac atggtactaa aatgtattta
    17041 attcaagatg aagatgggca agtgcaacta gcacattcta tttcagcagg acttgattat
    17101 cctggtattg gaccagaaca ttcttattac cacgacattg gtagagtaac ttttgaaaat
    17161 gctagtgata cacaagcaat gaatgcttta atcaacttta caaaacatga aggtattata
    17221 cctgcaattg aaagtgcaca tgcactgagt tatgttgaaa gactagcgcc tacgatgtcg
    17281 aaagaagata ttattgtagt aactatttct ggacgtggcg ataaagatat ggaaacaatt
    17341 agacaatata tggtagagcg aggtcttgca aatgactaaa ttatttatac cttatattat
    17401 gggcaataaa gatttgattg aaaatgcaac attgttgagt gaaaatggtg cagatataat
    17461 tgaaattgga gtacctttct ctgatccggt tgctgatggt ccagttatca tggaagcagg
    17521 tcaacaagcg attaaacaag gcatcacgat agattatatt ttcaatcaat tagaaaaaca
    17581 tggtgatcaa attaagtgta actatgtatt aatgacgtat tataatatta tttgtcatta
    17641 tggagaacaa gcgttttttg aaaaatgtcg agatactggt gtctacggct taattattcc
    17701 tgatttacca tatgaattat cgcagcgttt aaaacaacaa tttagtcact atggcgtcaa
    17761 aatcatatcg ttagttgcga tgactactga tgacaaacgt ataaaagata tcgtatccca
    17821 tgcggaaggc tttatttata ctgtgacgat gaatgcgaca acagggcaaa acggtgcgtt
    17881 tcatccagaa ttaaaacgaa aaattgagtc aattaaagcg atagccaatg tgccagttgt
    17941 cgcaggattt ggtataagaa caccacaaca tgttgcagat ataaaagagg ttgcagatgg
    18001 cattgtcatt ggtagcgaaa tcgttaagcg atttaaatct aacacgcgtg aggaaatcat
    18061 taaatattta caatctatcc aacaaacatt gaataattaa gtttacttga tttaaaaaaa
    18121 ttaggcgaat actgtttgaa aaagtgaaaa acggtgaatt ataaaattga atagaaatga
    18181 tacaaaagta atatgagcaa acccaaacgt tcatattact ttttttgaaa ttgtattcaa
    18241 aaatctaaat attactataa aagtatacgc aattaaagcg tttatgtttt agttttaaca
    18301 ttaactattg tatacttatt tagattagat ttattatttt tgacatttgc agaggggaaa
    18361 tagaaaaact gcaaatacgg aaatgaaatt aattaacgag agacaaatag gagtaatgat
    18421 aatgaagttt acaaatttaa cagctaaaga gtttggtgcc tttacagata gcatgccata
    18481 cagtcatttc acgcaaactg ttggccacta tgagttaaag cttgctgaag gttatgaaac
    18541 acatttagtg ggaataaaaa acaataataa cgaggtcatt gcagcttgct tacttactgc
    18601 tgtacctgtt atgaaagtgt tcaagtattt ttattcaaat cgcggtccag tgattgatta
    18661 tgaaaatcaa gaactcgtac actttttctt taatgaatta tcaaaatatg ttaaaaaaca
    18721 tcgttgtcta tacctacata tcgatccata tttaccatat caatacttga atcatgatgg
    18781 cgagattaca ggtaatgctg gtaatgattg gttctttgat aaaatgagta acttaggatt
    18841 tgaacatact ggattccata aaggatttga tcctgtgcta caaattcgtt atcactcagt
    18901 gttagattta aaagataaaa cagcagatga catcattaaa aatatggatg gacttagaaa
    18961 aagaaacacg aaaaaagtta aaaagaatgg tgttaaagta agatttttat ctgaagaaga
    19021 actaccaatt tttagatcat ttatggaaga tacgtcagaa tcaaaagctt ttgctgatcg
    19081 tgatgacaaa ttttactaca atcgcttaaa atattacaaa gaccgtgtgt tagtaccttt
    19141 agcgtatatc aactttgatg aatatattaa agaactaaac gaagagcgtg atattttaaa
    19201 taaagattta aataaagcgt taaaggatat tgaaaaacgt cctgaaaata aaaaagcaca
    19261 taacaagcga gataacttac aacaacaact tgatgcaaat gagcaaaaga ttgaagaagg
    19321 taaacgtcta caagaagaac atggtaatga attacctatc tctgctggtt tcttctttat
    19381 caatccattt gaagttgttt attatgctgg tggtacatca aatgcattcc gtcattttgc
    19441 cggaagttat gcagtgcaat gggaaatgat taattatgca ttaaatcatg gcattgaccg
    19501 ttataatttc tatggtgtta gtggtaaatt tacagaagat gctgaagatg ctggtgtagt
    19561 taaattcaaa aaaggttaca atgctgaaat tattgaatat gttggtgact ttattaaacc
    19621 aattaataaa cctgtttacg cagcatatac cgcacttaaa aaagttaaag acagaatttt
    19681 ttaggaaggg aattatcaaa acatgaaatt tacagagtta actgttaccg aatttgacaa
    19741 ctttgtacaa aatccatcat tggaaagtca ttatttccaa gtaaaagaaa atatagttac
    19801 ccgtgagaat gatggctttg aagtagtttt attaggtatt aaagacgaca ataacaaagt
    19861 aattgcagca agccttttct ctaaaattcc tactatggga agttatgttt actattcgaa
    19921 tcgtggtcca gtaatggatt tttcagattt aggattagtt gattattatt taaaagagtt
    19981 agataaatat ttacagcaac atcaatgttt atatgttaaa ttagatccgt attggttata
    20041 tcatctatat gataaagata tcgtgccatt tgaaggtcgc gagaaaaatg atgccctagt
    20101 aaacttgttt aaatcacatg gttacgagca tcatggcttt acaactgagt atgatacatc
    20161 gagccaagta cgatggatgg gcgtattaaa ccttgaaggt aaaacacccg aaacattgaa
    20221 aaagacattt gatagtcaac gtaaacgtaa tattaataaa gcgataaact atggtgttaa
    20281 agtcagattc cttgaacgtg atgagttcaa tcttttctta gatttatatc gtgaaactga
    20341 agagcgtgct ggatttgtat caaaaacaga tgattatttt tataacttta ttgacacata
    20401 tggagataaa gtattagtac cattagcata tattgacctt gatgaatatg tgttaaagtt
    20461 gcaacaggaa ttgaatgaca aagaaaatcg tcgtgatcaa atgatggcga aagaaaacaa
    20521 atcagataag caaatgaaga aaattgcaga attagataag caaattgatc atgatcagca
    20581 tgaattattg aatgcaagtg aattgagcaa aacggacggc ccaattctaa accttgcttc
    20641 tggcgtttat tttgcaaatg catatgaagt gaattatttc tctggtggtt catcagaaaa
    20701 atataatcaa tttatgggac catacatgat gcattggttt atgattaact attgcttcga
    20761 taatggctat gatcgttata atttctatgg tttatcaggt gattttacgg aaaacagtga
    20821 agattatggc gtataccgct ttaaacgtgg atttaatgta caaatcgaag aattaatagg
    20881 ggatttctat aaaccaattc ataaagtgaa atattggttg ttcacaacat tggataaatt
    20941 acgtaaaaaa ttaaagaaat agatgtaaat gatgttaggg cttatagtta ttgatactat
    21001 aggctctttt ttatatgttt tttaataaag cctcgaataa tgatatatta aatgacaagt
    21061 ttaacaagtg attgcaataa atataaatat gaattgcaca acctatttgt aaatgattga
    21121 gttgagtata ctttttaatt cattcaatat gactcaaaac aagccaatat gtattatatt
    21181 tttggtgaat aaaccttagc ataacaattt atgtctgatt atatatgtgt gtgtttgcaa
    21241 gtgtgtgata tcaaatgtca tatttgtcta attgtaattt ataaaatgca tgagcaatga
    21301 ttgctgtaac aaattctagc aaagtattca gtttgtattt tagaaataaa aatgatgata
    21361 gatgtatgat cgatataaaa gatttaatat gcaccaaaat gataacgtga gtaattggtt
    21421 taatgaaaca taacatgaat gtaaatttag cttactgaca taaacactac acaaacatac
    21481 agttatataa gtccccttat ggaatttcac attctagttt acataatata tattatagga
    21541 agttatatgt gtgtaacgca aaaggtaccc tacatcataa tcattatcta atatcgtcac
    21601 ataacttact tatgctataa tcatggtatt atattgtttg gagtgatttg atgagatttg
    21661 tctttgatat tgatggtacg ctttgttttg acggccgatt aattgaccag actattattg
    21721 atacattgtt acaattacaa catgatggtc atgaacttat atttgcatca gcacgtccga
    21781 ttcgtgattt gttgccagtt ttaccatcag tatttcatca gcacacatta attggcgcaa
    21841 atggtgctat gatttcacag caatcaaaga tttctgttat caaaccaatt catactgata
    21901 catatcatca tatcttcaaa ataattcaaa agtatgagtt agattatatt attgatgatg
    21961 attggaatta tgctgcacaa cttgacgctg agaacgcgat ttttgagcgt ttagatccac
    22021 ataagctggc cagttgtatt gatgttgcaa atatcgacac accaatcaag attattttat
    22081 taaatataga cccggcacaa attacaacta tattagacga gctagataaa taccatcaag
    22141 aattggaaat gattcaccat tcaaatgagt ataacattga tataacagcg caaaatatta
    22201 acaaatatac tgcattacaa tatatatttg atgcagatgt taaatatata gcatttggta
    22261 atgaccacaa tgatattgtc atgttacaac atgctagtag tggctatatt ataggaccat
    22321 cagaagcata cacacacgca atattgaaac ttgataaaat caaacacatc aataataatg
    22381 cacaagctat ttgcaaagtc ttaaaatcat ataaataaaa acacccctat caaatgataa
    22441 tcattatcaa tcgatagggg ctattttaat aaaattcgtc ctcgaacatt tcttcctctt
    22501 catctaatcc aaataattct gccatttctc catgttcaat taacatgttt aaatatgcat
    22561 cgcggagttc ttcttcactc atatcattaa tcatttcttt aagactatca atccacatat
    22621 ttctgcgtaa ttgatagtct tcttcaactt cgtttaacat cattatatgt ttatttgctg
    22681 cttctggact agctgtaaag agtaatgcaa tcatatgttt acatatcact cgtcttccat
    22741 cagcatgagg acaattacat atggattttc taggatgttc catatcaata taacaacgat
    22801 atactttgtt gccactgccc tttacttcag cctcatgctg cgtttctgaa aatgatttta
    22861 agttaatgac gcattcactt tgataataat taaagcctct ttctatagaa cgaatacttg
    22921 caatatcaag taatcccatt aatgatactc ctttttatta ttatttttaa ataaagaaaa
    22981 taaaatagat aagtgtctag attaaaatac ttgatttatc tatattttat aacaagtcta
    23041 gaattatcgc attcttaaat aactaatatg aaaatgcttg cactaattct tttgtataag
    23101 ggtgtctatc aacattaaat aattcctcta ttgcaaaatc atcgactatc atgccatcct
    23161 taagaacgat aattctatta actaagcgtt gtaacacgga taaatcatga gaaataacga
    23221 taaaatgatt taagttcgta atcgtttgcg cttttaatat attgattaca ttttgttcag
    23281 ctataacatc taaatttgaa gttatctcat cacatattaa aacgcgaggc tgtgctaata
    23341 acgaacgcat gacattaaat ctttgtaatt gtccgccact cacttcgctt ggtaatttag
    23401 tcaataattg cgcgtttaac tcaaaagtag ataaatgttg taataataat tgatcctgag
    23461 cagtattatc agttagacct ctgtaataat ataacgcttc ttttaatgag gtctcaatcg
    23521 tccaatcagg gttaaagcta gttaaagggt gttggaaaat cggtaacaca gcattgtcac
    23581 ttaagtaaat ctctccttta acaggtttaa acaagccaag aaccaatgaa gcgagcgtac
    23641 ttttaccaca gccactttcg cctaaaatac caacattttc tccatcaggt atagtaatat
    23701 tgatatcttg tagcaccatc tgctttttat tataaccaaa agtcacatgt tttaactcaa
    23761 tcatagtaat ccctctttaa ttgtgttcta tatttaatta gacgttcagt atacggatgc
    23821 aaatgctcat acttgaaatg attaatatta cctcgttcaa tgatttgacc ttcttttaaa
    23881 acataaatgt actgacaata tttcaataca tgacttaagt tatgtgtgat aataaataat
    23941 gtttgaccat gttctaatac aatatgctgt aataaatcca tcacttgatt accgttcaaa
    24001 gcatccaatg atgcaactgg ttcgtctgca atgattaatt taggctccaa catgagaacg
    24061 cttgctatgt atacgcgttc aagttggccc ccagaaagtt ggaaactata tttatttaat
    24121 atatctttgc tttgtaaatt aacccacgac aaagccttat caactttgga caaagcctct
    24181 tctttactac ctttataatg cttacgataa atcgcagtta actgtttacc taatttagta
    24241 tggtcgttaa aactttctgc ataattttga gaaatatagc caattgtatg accataatat
    24301 tgactcaatc tactaacatt ttccccatca aattggtacg aatcatacgt gcagcttaaa
    24361 tcaaatggta aatattcaag taaagcttta gcaatcaaac tttttccagc gccgctctct
    24421 ccaatcaagg cattaatctg ttgactaaaa attttcaaat caatcccttt aataagagat
    24481 ttctcactag tattctttat tgttaaattt tgtatatcaa tgagactcat catattcacc
    24541 ccgttgtttc agcaatctat ctcttagtgc atcaccggtt aaattaaaaa ttaaaatagt
    24601 tatagcaatg actgaagcag gtgcaatcaa cataattgga tgagacgaaa taaaatcacg
    24661 accttgttgc aacatagcgc cccactctgg tgttggcggt tgtgcaccta acccaataaa
    24721 tgatagtgaa cttatatata gaatgatttt accgaaatca acgaccatca aaacgataat
    24781 agccggtata attttaggtg ttaaatgacg tattaatatt gttcttgttg gtacatgaaa
    24841 taattgtgcc atttttatat aaggcttatt catttcgcta ttaactatac ttctagtcaa
    24901 ccttgtgtaa ttcatccatt ttattaatgt aattgagata actaaattcc ataaagatgg
    24961 ttgaaaaaaa cttgctaaag caatcatgat gataaattct ggaatactta gaccaacatc
    25021 aataaacctt aacactaatc gttcaatcca cccttttttg tatccggcaa atagacctag
    25081 tgtaacacct atgacaacga tagctattaa tgttaaaaca gtaacaaaca atgttgaacg
    25141 tgcaccgata ataattcggg taaataaatc tctcccataa tcatcagttc ctaataaatg
    25201 caaccaacta ataggttcaa aagtttgtga taaattgact ttggttgcat tttcactact
    25261 gacaaagaat tgcagtacaa ttaccacaaa aataaatgca acgaatacaa aaaatatcag
    25321 gttattcttt gaaaatattt tatgcatgac ggtcactact ttctgatatc aatggtgtat
    25381 tggttttgat ttttggattt cctaattgta aacgctgctt cggatcaagt aataacgtta
    25441 ataaatcagc aatcgtattg ataataacaa cgaagaagcc aataaataac acgcatcctt
    25501 gaataacagg ataatctcga gatttaatac tatccattaa tagataacca ataccaggta
    25561 tatcaaataa attttcaatc actacagtac cacctattag actgccaagt gaaatcccta
    25621 gtaatgggat aatcggcaaa attgttggtt ttagtaaatc atgaattaaa atataacgtt
    25681 cattcatacc gcgtaatctt gatgcttgta cgatattact ttgcaataac atcaataaat
    25741 tagaacgcac taaacgaatg atgtatgcac acatacctaa agatagcgtg attacaggta
    25801 atataaactg acttagtata acgctatcta tattcattaa atttgtgaca ataaataata
    25861 aaataatacc gataaagaac gctggtaaac taatcgatag tgttgagatc actctaatca
    25921 ctttatccgt ccacttatga aatcgtttgg ctgctataat gccgagcggt atagatatgc
    25981 ataacgacac tactaatgtt gaaaatgata tgagtaatgt tatgggtgca tagttgaata
    26041 atatctgtgt taccggttct tttgattcaa aactttttcc taaattaaaa tgtaataaat
    26101 gattcatcca atgccaccac tgtaccaata aagaatcatt taatcccaat ttatctttgg
    26161 ttgcatttat ttgttccgtc gacacttgtg ctacatcaag atgtaatatt ttatcaacag
    26221 gattgcctgg tgataatttc attaaaatga atgtaagtgt agaaataaca aataaaacaa
    26281 ctatcatttg catcagtcta tacaacatag actttattat gaacataata gtccccctcc
    26341 ttgtgtaagt tactaacact ttctttttac atgagaatgg cgcatgtata tgcaacttac
    26401 atattaagaa ctaacgttca ttatagtatt atccataaag aaattgaagt atatttaatt
    26461 ttttaacaaa atcattataa aatataatat tttgaatcaa gtcaaccatg taaaatataa
    26521 aaaagtcaaa acaaaaacaa ctatagcact gtattccatc tctttcgaaa taattgttac
    26581 tgcagtgtaa cttaaaagtc gatgattttg tgcatatagt tgtcgaatat tattttttat
    26641 ctttacggcg aagttcagcg ccctcatagc cgtatttttc aatttgcttt tctaatttac
    26701 gcgcttttct ttctttacgc caatttctag taaaatacca taatagaaaa ctaattaata
    26761 aactcataat cgctaaaaat gcagcgtatc ctaataatgg ttgatatttt atatcttgaa
    26821 aatttggaat aaaaaatgca agcacaccta atataacaaa tgtaattact gcagatacaa
    26881 accatttatt taaaactaag caacagaata ttgttaataa aatcattatt aatgttgtga
    26941 tccataaata attaggcata tcgaataatg tcatattcat tctcctttta tttcattact
    27001 ttccttgtat acattttatt ataaattttt aaaaacttaa acaatagcag tcagtttcaa
    27061 gcaatattct atctactaat agaaaaatca ttgttccttg cgacatggaa atcgtaacat
    27121 tatcgtttag gagacaaaat tatgtataat gaatgtatta taccaaagga gtgattatat
    27181 gtctcaaggt ttacctttaa gagaagatgt tcctgtttca gaaacatggg atttagtaga
    27241 cttatttaaa gatgatcaac aatattatga aagtattgac gctctagtac aacaagcaaa
    27301 tcaatttcat catacatatg caacaacatt aaattcaatc gaacaaatta atactgcttt
    27361 agctgaatta gaaaatattt taattgcctt agatcgctta agtaattatg cagaactacg
    27421 tttaagtgta gatactagta atatcgaggc acaagtattg agcgctaaat tatctactac
    27481 atacggtaaa attgttagcc aattatcatt tgtagagtca gaaatacttg aattaccaga
    27541 agaaatactt caacaattag aagaatcatg tccatatcaa cactatatta aacagttaat
    27601 aaaacaaaag ccattccaat tatctgcgtc ggtagaacaa gtattagcaa ctttatcacc
    27661 tacgctaaac agtccttacg atttatacgg cacgacaaaa atgctagata ttacattcga
    27721 ttcatttgaa catgatggta caacgtaccc tgtcgactat gctacgtttg aaaatgatta
    27781 tgaagataat aaagatcctg agtttagacg taaaagtttc aaatcgttta gcgatgggat
    27841 tcgaaaatat cagcatacta ccgcggctac atataatatg caagtacaac aagaaaaaat
    27901 tgaagctgat ttacgtggat ttgaatcagt catcgattat ttattacata gtcaagaagt
    27961 aacgcgtgat atgtttgacc gtcaaatcga tatgattatg cgtgacttgg caccagttat
    28021 gcagaaatat gctaaacttt tacaacgtat tcacggatta gataacatgc gttttgaaga
    28081 cttgaagatt tctgtagacc ctgattatga accagagatt tcaattgaag actcaaaaaa
    28141 ttatattttc ggtgcgttaa gtgttttagg tgatgactat acaaacatgt tacgtgaagc
    28201 atacgatcag cgatggattg attttgcaca aaataaaggt aaagatacag gcgcattttg
    28261 tgcaagtcca tactttacac attcatatgt gtttatttct tggactggta aaatggctga
    28321 agcatttgtc ttagcacatg aattaggtca tgcaggtcat tttacattag ctcaaaaaca
    28381 tcaaccatat cttgaatcag aagcatcaat gtactttgtt gaagcccctt ctacaatgaa
    28441 tgaaatgttg atggccaatt atttatttaa cacaagtgat aatccaagat ttaagcgttg
    28501 ggttattggc tcaattttat ctagaacata ttatcataat atggttaccc atttattaga
    28561 agctgcttat caacgtgaag tgtatcacaa agtagatcaa ggtgaatctt taaatgcgcc
    28621 gacattaaat gaaataatgc taaatgttta taaacaattt tttggagatg cagtagacat
    28681 gactgagggt gctgaattaa catggatgcg tcaacctcat tactatatgg gattatattc
    28741 gtatacgtat tctgctggct taacaatcgg aactgtcgtt tctcaaaaga ttaaaaatga
    28801 aggccaacca gctgttgatg cttggttaga aacattgaaa aaaggtggta gtgtatcacc
    28861 tgtcgaactt gcaaacattg caggtgtaga cattactaca gaacagccac ttaaatctac
    28921 aattcaatat atttctgatt tagtcgatga agttgaaaaa ttaacagatg aaattgagca
    28981 agcaaataac taagatgcga gacaatttat agtagaacaa tataaaccaa gattttcaca
    29041 ttactaattg tgagaatctt ggtttttttg agggtttatg aataatagaa atatatattt
    29101 atcgtaaggg attttatagt aataacttta agttattgtt cgtaatgtgt acctgttaaa
    29161 taaaaataaa cactttcagc gatgttaata atatgatcac caatacgttc taaatgtctt
    29221 gctgctaaat gagcttgtgc agcgacaaat ggatcgttat caataagata cgttgcgtta
    29281 ataatatgac tatataagtc atcgatatct tcatcacgct caattatttc tcttattaat
    29341 acggtatctt tctttttaaa tgcttgatct aagtccttta acattaacat agctaattta
    29401 cccattgtct ttaaacgggt taacacataa tcatctgtaa tctttgtacg caatcgaata
    29461 ttggcaatac tcgaggcatt atctcctatt ctttctaaat cggaggcgat ttttaatgaa
    29521 gaaatcatca tacgcaaatc actcgcaatg ggctgttgct ttgtaattaa catgataact
    29581 cgctcattaa tatcataatt taattgattg atatgtttat cgtttttaac tgtttgtcgt
    29641 gcaaagcctc tatcgtcaat acttaatgat tttataccat tttcaatact cacatagaca
    29701 tttgcaccta accgacgtaa ttcttttatt aaatcatcaa gttgctcctg atatcgttgt
    29761 ctaattattg ccattatata tcaaccaaac cttcctgaaa tataatcttc tgttttcttg
    29821 tttgatgggt tagagaaaat tttatcagta tcatcatatt cattgacata accatttaag
    29881 aaaaatgcag ttttatctga tacacgagct gcttgttgca tattatgtgt aaccataata
    29941 attgtatact tttcttttag ttcttgaacc aactcttcta ctcttaatgt tgagattgga
    30001 tctaatgctg atgtcggttc atccattaaa atgacttcag gttcaattgc taaacaacgc
    30061 gcgatacaaa cacgttgttg ttgcccaccg gataaactat atgcatttgt gtgcaaccta
    30121 tcctttaatt catcccaaat tgcagcgcca cgtaatgatt tctcaacgat ttcatcaaga
    30181 acttttttat ttttaatacc gtgaatcttt ggaccgtaag taatattatc gtatattgat
    30241 tttggaaatg gattaggttg ttgaaagacc atgcccacat ttgtacgtaa ttgttcttta
    30301 gaatattttt gatcaaaaat gtcttgatct cgatataata ttttaccagc tgttttcaca
    30361 gaaggtacta actcaaccat tcgattcaaa gttttaatat atgttgattt gccacaacca
    30421 gatggaccta taatggcagt aatttggttt tcataaatat ctaaattaat attttgtaat
    30481 gcatgatttt cgccatacca taagtctaaa ttttgtgttg aatatataac agaatgtgag
    30541 tttgtatcat ctggtgtttt atgatgactt tgtgagacat caaacgtatg actttgagat
    30601 atttgtttag tttgtgcaag tgtttgcgcc atatttaaaa ctcccttttt aatagataaa
    30661 tgagattgat tgtttaaatt agaatttttt actaaatttg ttacgtaaaa taatcgcaac
    30721 gccattcatt aagattaaga taactagtaa aacgataatg cctgccgatg caacattctg
    30781 gaattcttct tgaggcattt tcgcccaagt aaatatttgg ataggtaatg ctgaaaattg
    30841 atccaatata cttctaggtg ttgccaataa tatagtcggt ataccgatta gcacaagtgg
    30901 cgctgtttct cccagtgctc ttgaaagaga caaaatgaat ccagttaaaa taccaggtaa
    30961 cgctgctggt aagacaacac gtcttatcgt ttgccattta ttagcaccta agccgtaaga
    31021 agcttcgcgt actgagttag gtacagctct aattgcttcc tgacttgaaa caataataat
    31081 tggtaatatc agtaaggtca ttgttagcgc tgctgccaat atactgttac ccattttcaa
    31141 ggcttcaatc cctgcaccac caacgaacaa agtataacct aataacccaa atacaactga
    31201 tggtacacca gctaaattgg aaatactgat tttaacaaac tgagtaaatt tgttgttttt
    31261 cgcatattct tctaagtata tagctgtacc tattcctagg atgattgata atggaatgat
    31321 acttaacatt aaccaaagtg aaccgattaa cgcgccttta acgccagcca tagatggtgt
    31381 tgaagaagaa aaattagtga aaaactgtaa atttaaatga cttacccctt taatcaatgt
    31441 ttgagttaac aacgcaataa gtacgacaag tcctaataat gtacatgcta aaaatatgag
    31501 tttgaacact ttatttttaa ccgttctgga tgataaatgt ttttggacaa gttgttgatc
    31561 gacgagtgat tgtctattat tatctgtcgt ttccatatta atactcctcc ctaaaacgct
    31621 tagaaatcca ctgagaaagt aaattcatga ttaaggtaaa gataaatagt gtgaacccta
    31681 cagcataaat actgtaataa atatttgatc caaatgttgc atcacctgtc gctatctcaa
    31741 caatatatcc agtcattgtt tgaatcgaac ttgttaaact taatgaagct gttggcgaac
    31801 tacctgccgc taatgataca atcatcgttt ctccaattgc tcttgaaatc gcgagaacga
    31861 ttgaagctac aatacctgat gttgctgcgg gaagtacgac tttagttgct acttctaatt
    31921 tagttgctcc aagtccatag gcaccttctc gaattttatt tggtacagat gccattgcat
    31981 cctcactcaa acttgtgatg agagggacaa tcataatacc gacaactaag ccgggactta
    32041 tagcattaaa ctctccaaga cctggtatga aagatcttaa tactggtgta acaaaggtta
    32101 atgcaaagaa accaaacaca attgttggta ttcctgctaa aatttctaat atcggtttaa
    32161 ttatgcgtcg tgcacggtca cttgcatatt cacttaaata aattgctgca ccaagcccga
    32221 ctggaactgc aaatatagtc gcaataactg tgatttttaa agtccctatt atcaatgccc
    32281 agataccaaa cttagggtct gaaccggtag gattccaagt agtagaaaat agaaattcag
    32341 ttattggaat tctggtgaaa aaagtgatgg tttctaaaag caatgtgatt aatataccta
    32401 gtgttgttaa aatggaaatc gctgaaattg cggctaaaat aactggtata attttgtcat
    32461 tatgctttcc ttttttatta ttattttttt cgattaaagc tttaacatta gtagatgaag
    32521 tcataatcaa acctctttct attactccca aaataattag atttgtaaaa taatgatgaa
    32581 ccactatttt aagttgataa gcttgtagac taactgaatg tcaaaatcag cgtctcgata
    32641 aatcaagctt tgtactctaa ataatgattc accatcaaaa tcatgtttca agacacattt
    32701 gaaattgcgt cttattattt tttgtcttca gactttttat catcagattt cttgtcgtct
    32761 gatttttgat ttttatcaat aaatgctttt aaatcatcta attgtgattt gtatgttttc
    32821 tctggtgctg ctacatatcc agcttcttca gctgctttac ctttatcttc taagacgaat
    32881 ttgataaatt ctgacattac tttattatct ttcaatgctt tttcatttac ataaatgaat
    32941 aatggtctac ttaatgcata agagttatct tgaattgttt ttttcgtagg ctctgttgct
    33001 ttaccatttt catctttgat tttaacttct tttaatttat ctttattttg tacgtagaag
    33061 ttatatccaa agtatccgat tccctctttg tttttcgtta cagaagaaac gatagcattt
    33121 gtatcagcat ttttttctgc tttaatatct tctttattca ttacttcatt ttcaaagaag
    33181 tcataagtac catgacttga gtttggtgat acagcattta tttttttatc tggccattta
    33241 ctattaacat ctttccatgt tttagctttt ccagaataaa ttgcttttaa ttgctgtttg
    33301 tctaattcat ctacaaaatc attttcttta tttacagcaa ccgttacacc atcttgcgca
    33361 attttgaatt ctttgtattt gatattctta tcttgtaatt tttgcttctc ttcatcttta
    33421 attggtctag aagcatcagc gaagtcgata tctcctgcaa tgaatttttg gaaaccagca
    33481 cctgtaccag cttgtcctgc tgagatttta gcatccgagt gatcttgagc ccatttttca
    33541 tttaatttct ccacaattgg tgctactgtt gatgagccat cacctttagc ttcccctttt
    33601 aaatcactat taccactgcc accattaccg ccaccacaag cacctaataa tagtgttgca
    33661 cctaaagctg tagtaccaac aaattgccat tttttcattg aaatatcctc cctgtatgaa
    33721 caacaaatag tatttgtatt gattacatct ttatcttaac cttgaattat taacctcatt
    33781 tttagaaact gtaaatgtta cataaaaaat tgttaagaaa atgtaaatat taatttttgt
    33841 taatctaaaa aatattgctt cattagtaaa attaaaatgc ttcctgaaaa atcatcgaaa
    33901 tttaaatatg aatacaaaaa
//
LOCUS       DALAGF010000016        33821 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.016, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000016 DALAGF010000000
VERSION     DALAGF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33821
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.016"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..288)
                     /locus_tag="O2692_000730"
     CDS             complement(<1..288)
                     /locus_tag="O2692_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCZ4298371.1"
                     /translation="MIINLETQQIHFDLEHEIPYFQAPEKNRIRLDIDVLNKKQISNI
                     INVIFNNQSKTKCTFLSEYLYPVKFREKTRIGRFFNITNWYEEIHSTDEKYV"
     gene            complement(290..376)
                     /locus_tag="O2692_000731"
                     /pseudo
     CDS             complement(290..376)
                     /locus_tag="O2692_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            391..717
                     /locus_tag="O2692_000732"
                     /pseudo
     CDS             391..717
                     /locus_tag="O2692_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            746..937
                     /gene="vraH"
                     /locus_tag="O2692_000733"
     CDS             746..937
                     /gene="vraH"
                     /locus_tag="O2692_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HCZ4298372.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALTILVIVLSSFTTPMIGI
                     PAGLLGGSYYLKRREEKGK"
     gene            1330..1530
                     /locus_tag="O2692_000734"
     CDS             1330..1530
                     /locus_tag="O2692_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCZ4298373.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(1657..2226)
                     /locus_tag="O2692_000735"
     CDS             complement(1657..2226)
                     /locus_tag="O2692_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ4298374.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGVTVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVIGILSLIIFLSVYLANKFL"
     gene            complement(2486..2881)
                     /locus_tag="O2692_000736"
     CDS             complement(2486..2881)
                     /locus_tag="O2692_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCZ4298375.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIVVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            complement(2949..3302)
                     /locus_tag="O2692_000737"
     CDS             complement(2949..3302)
                     /locus_tag="O2692_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCZ4298376.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            complement(3755..4594)
                     /gene="noc"
                     /locus_tag="O2692_000738"
     CDS             complement(3755..4594)
                     /gene="noc"
                     /locus_tag="O2692_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HCZ4298377.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(4637..5356)
                     /gene="rsmG"
                     /locus_tag="O2692_000739"
     CDS             complement(4637..5356)
                     /gene="rsmG"
                     /locus_tag="O2692_000739"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCZ4298378.1"
                     /translation="MTVEWLAEQLKEHNIQLTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(5356..7233)
                     /gene="mnmG"
                     /locus_tag="O2692_000740"
     CDS             complement(5356..7233)
                     /gene="mnmG"
                     /locus_tag="O2692_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCZ4298379.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILELLEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(7300..8679)
                     /gene="mnmE"
                     /locus_tag="O2692_000741"
     CDS             complement(7300..8679)
                     /gene="mnmE"
                     /locus_tag="O2692_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCZ4298380.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVIEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(8818..9171)
                     /gene="rnpA"
                     /locus_tag="O2692_000742"
     CDS             complement(8818..9171)
                     /gene="rnpA"
                     /locus_tag="O2692_000742"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCZ4298381.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(9292..9429)
                     /gene="rpmH"
                     /locus_tag="O2692_000743"
     CDS             complement(9292..9429)
                     /gene="rpmH"
                     /locus_tag="O2692_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCZ4298382.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            10160..11521
                     /gene="dnaA"
                     /locus_tag="O2692_000744"
     CDS             10160..11521
                     /gene="dnaA"
                     /locus_tag="O2692_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCZ4298383.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            11799..12932
                     /gene="dnaN"
                     /locus_tag="O2692_000745"
     CDS             11799..12932
                     /gene="dnaN"
                     /locus_tag="O2692_000745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCZ4298384.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
     gene            13313..13558
                     /gene="yaaA"
                     /locus_tag="O2692_000746"
     CDS             13313..13558
                     /gene="yaaA"
                     /locus_tag="O2692_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCZ4298385.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            13555..14667
                     /gene="recF"
                     /locus_tag="O2692_000747"
     CDS             13555..14667
                     /gene="recF"
                     /locus_tag="O2692_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCZ4298386.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            14677..16611
                     /gene="gyrB"
                     /locus_tag="O2692_000748"
     CDS             14677..16611
                     /gene="gyrB"
                     /locus_tag="O2692_000748"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCZ4298387.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            16648..19311
                     /gene="gyrA"
                     /locus_tag="O2692_000749"
     CDS             16648..19311
                     /gene="gyrA"
                     /locus_tag="O2692_000749"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCZ4298388.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDSSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFENLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEE"
     gene            complement(19398..20210)
                     /locus_tag="O2692_000750"
     CDS             complement(19398..20210)
                     /locus_tag="O2692_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCZ4298389.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            20536..22050
                     /gene="hutH"
                     /locus_tag="O2692_000751"
     CDS             20536..22050
                     /gene="hutH"
                     /locus_tag="O2692_000751"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HCZ4298390.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQSEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYDEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
     misc_binding    22123..22339
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            22429..23715
                     /gene="serS"
                     /locus_tag="O2692_000752"
     CDS             22429..23715
                     /gene="serS"
                     /locus_tag="O2692_000752"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCZ4298391.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     gene            24365..25060
                     /locus_tag="O2692_000753"
     CDS             24365..25060
                     /locus_tag="O2692_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCZ4298392.1"
                     /translation="MTTHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIVL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMEQ"
     gene            25057..25386
                     /locus_tag="O2692_000754"
     CDS             25057..25386
                     /locus_tag="O2692_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCZ4298393.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     regulatory      25591..25689
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            25749..26717
                     /locus_tag="O2692_000755"
     CDS             25749..26717
                     /locus_tag="O2692_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCZ4298394.1"
                     /translation="MTNYTVDTLNLGEFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     gene            27008..27946
                     /locus_tag="O2692_000756"
     CDS             27008..27946
                     /locus_tag="O2692_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HCZ4298395.1"
                     /translation="MGVAYVFSKIQPKATIIATIALVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LVIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            27961..29928
                     /gene="gdpP"
                     /locus_tag="O2692_000757"
     CDS             27961..29928
                     /gene="gdpP"
                     /locus_tag="O2692_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HCZ4298396.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIILFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            29925..30371
                     /gene="rplI"
                     /locus_tag="O2692_000758"
     CDS             29925..30371
                     /gene="rplI"
                     /locus_tag="O2692_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCZ4298397.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            30403..31803
                     /gene="dnaB"
                     /locus_tag="O2692_000759"
     CDS             30403..31803
                     /gene="dnaB"
                     /locus_tag="O2692_000759"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCZ4298398.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            32081..33364
                     /locus_tag="O2692_000760"
     CDS             32081..33364
                     /locus_tag="O2692_000760"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCZ4298399.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
BASE COUNT        11484 a         5525 c         6037 g        10775 t
ORIGIN      
        1 aacatacttc tcatcggtag aatggatttc ttcgtaccag ttagtaatat taaaaaaacg
       61 accaattctt gttttttctc taaatttaac aggatataag tattcagaca aaaatgtaca
      121 tttcgtcttt gattggttat taaatataac attaataata ttagaaattt gctttttatt
      181 gagaacatca atatctaagc gtatcctatt tttctcaggt gcttgaaaat agggaatttc
      241 atgttctaaa tcaaaatgta tttgttgtgt ttcaaggtta ataatcattt taatctccaa
      301 ttctttttag gttagcatcg tatgtaccac ttcttgtttt cttgctacct aaattcttaa
      361 tactgcttgc accttttcat tttatcttct catatattta aatcaattac ttttgattgt
      421 ggtaaggagt tctcaaactg gaaaacaata tgtaactgtc acgatatatc tatatttttt
      481 gctgatccag gcacgccatc acagcgaggt ttaaatgaac attctaacgg aataattaga
      541 cgcagtggat tagataaaga actagatttc aatttagtaa cggatgatca tatcatcagt
      601 gttgctcaaa aaatcaatca tcatcctaga aaatctttag gttatagaac accattggaa
      661 gtatttatga gtttcatcga agatgataaa cttgtccagc ttaatttgac aatctaaaga
      721 tttataaaaa aggagagatt gaatcatgaa atttagagaa ttagtaaaac aatcatatga
      781 ggatttgaaa aatttgactg taacttggta taacgtcatt gctttaacaa tacttgttat
      841 tgtattaagc tcattcacaa caccaatgat aggcattcca gctggtttat taggtggctc
      901 ttattattta aaacgtcgtg aagaaaaagg caagtaagca cattccatcg gtatagaaca
      961 ttgctatgac ctttcaagtc agattttaat atcaacaagt agcgtttatc gctgcttgtt
     1021 ttttatggat tgcttataaa atcatcatgt ttaaacgata tgtcactttg aaaactagca
     1081 aacaccataa aagactacaa tttttttatt tttataaacc cctttaaatc aacgtttcta
     1141 aggtttttaa aaatcttttt taatttattt tcaaaaaaca ctgtacatta tgccaatatg
     1201 agcgtatagt tggtcttacg taataaaagc tcgtgaatta aattgtagtg tatttgttta
     1261 gaatatcctc ttttttagtt atgaatttgt tacaaatatt aagtgcaaaa gcacacggag
     1321 gttttctata tgaataacgg tacagtaaaa tggtttaacg cagaaaaagg ttttggtttc
     1381 atcgaacaag aaaatggcgg agacgtattc gtacatttct caggtatcgc tagcgatggc
     1441 tacaaaactt tagaagaagg tcaaaaagtt actttcgaaa tcactgaagg tcaacgtgga
     1501 gaccaagcag ttaacgtaca aactgtttaa tcttacaaca taaaacgact cattataaat
     1561 gaatcgcttt gattaccaac aaggttctaa atcttgttgg tattttttta tgctattttg
     1621 gataatataa ataatatgag acgataccac gatgatttat aaaaacttat ttgccaaata
     1681 cacactaagg aaaattatga ggctgagtat accaattact gatataatag aaagcgcatt
     1741 tgtcatcgta tttctagctt tagtcgtttg tacttcacta ggatcttttc cattcataaa
     1801 agcaacaatt cgatcgtagt tgtccatttt atgcactttt tttaaatctt ctattttcat
     1861 agatgcataa aaacctattc ccaaaatgat tgccaccgtt acaccccaag tccaattcca
     1921 ataaacaact aaaggtccca ttaaaattgc agctaatgtc atgaaaataa gcatcacata
     1981 tgtccataag ttaagacttc gttgcgcttt aatatcaggt acaaatggaa tggtcccttt
     2041 tactaaatca tctaaagtta cattgaacaa ttcacacatc attaataagt tatgtatgtc
     2101 tggtaagctt ttgtcatttt cccaattaga aatactctgc ctagatacat ataacttttc
     2161 agcaagatat tcttgtgaat aaccatctcg ttccctatac tttttaattt gtttactcag
     2221 attcaattac atacgcctcc agtcaattag tacaatcatt acaatcagac tatcagataa
     2281 agttaaatca agtcaccttt atgattgact aaatgttttg acatacgaca acatcggggt
     2341 tcgaagactc ccttaagaca gacctgtatg attacgcgct tgtaattttc tcaatgccaa
     2401 cttgaatttc acttattaat gatgcaactt ttccatgcta ttcaatgctt gcaaagttag
     2461 tcctagttaa gagattacgt tcacactatt tactattcga ttttccagct ttccaactat
     2521 gcttttctaa tcttttttgt gctataaatt ctaccgcttc aaaaatacat actgcactga
     2581 tgaaccagct taggaaacca acaacaatgc taatcaacca catgtgcgtc atatgtaaca
     2641 tcatccatgt aactaaaata caaactaata cccctgtcat accaaacact gcgccacgac
     2701 ttacatgcac agcgacttta tcaccatatt gcatacctgt aataaacata gacactaaaa
     2761 atactgccgg aaacgttgca aatataccgc caaattcttt ccaaggtagt gttacagata
     2821 caatataact taataatact gcaaatcccc ccacaaaaaa tttcattaat gttaatttca
     2881 tgatgttctg cctccaataa tttattactt aataactgaa tacaactaat tatcacgcat
     2941 tttccaaact ataaatgtct aatactgaat attgcgaggg aaatgacaaa ccaacaaaca
     3001 actgtgaata ttgcgccttt ccgataacct ttatgcttaa tgtagagaga tgtcaaaaat
     3061 acagttaata tacaagacag aataccgaca attgctccag tactaagatt catcgacatc
     3121 tccactaatt gcgtaccacg atgatctaaa gctaatgcga taatagccgc aagaaagact
     3181 gccggcatag tagctataat acctcctaac ttaccaccta ccttatcagc aataattgaa
     3241 gctaatgcaa cagcgatacc accaatgaca aaatgaagaa ttgcacttcc aattgaaaac
     3301 atcttcaacg tacctttcta taattaattc aatacaaaac gtatcattca cacaaagtgt
     3361 aaaacttaaa ttttctactg taaaggcaac tcaagcgatg ttcataaagc tttcatgata
     3421 ctttcataaa ctagcaataa agggttcaaa tgaaagtata tccagctcaa attagggagt
     3481 gggacagaaa tgatattttc gcaaaattta tttcgtcgtc ccaccccagc aaggttgact
     3541 agaattgaaa aaagcttgtt acaagcgcat tttcgttcag tcaactactg ccaatataac
     3601 ttcgtagagc atagaacatt gatttatgta ccagcctgat caacatataa atataaattt
     3661 ttatgtttca cgtaaaacaa aaaaaaagac aaagctgtta tgatcttagc tttgtctttt
     3721 atgtgttagt catcatgtat acgacatcct actactaacg tttatatatt cgaattttta
     3781 tttcataata atcttcatga tctttgtctt tatgctcaac atgtaatcct gtttgttgaa
     3841 tcgcttggat acttttgcct acctcatctc gtgcttgagt gacatcttgt gcaaagcgaa
     3901 ggttttgtgc tttgactttt tcgggccccg ttttttggcg tactctatct tcagtctgtt
     3961 tcacatttag cttttgtgca atgacttgct caatcaacgc ttcttgttcg ctatcagaca
     4021 atgataatac cgctctcgca tgacgttcag taattttacc ttctcttaag cgaagtagta
     4081 ctttcggcgc caacttcaat aaacgcaact tatttgcaat aaagctttga cttttaccta
     4141 aactttttgc caattcactt tgcgttgtat caccaatttc caataatttc ttataggctt
     4201 ccgcttcttc aacaacagac aaattttctc tttgaatatt ctcaattaat gcaacaacag
     4261 ccgtctcttc atcatccata tcacgaataa taacgtctgc ttgaggtaaa tttagtgatt
     4321 gtattgctct aaatcggcgc tctccagcaa taatttcaaa catatcttct tcaatcggtc
     4381 ttacaacaat aggttgtagt aaaccatgtt catgtattga ttcagcaagt tctttaattt
     4441 tatttggttc aaacacctgt cttggttgat aacggttggg aacgatacgt tcaatttgaa
     4501 tggattcaac attactattg cgatcttctt caatatgtcc aatgatgtca tctttgtttt
     4561 tcaaaccaaa taattttgaa aaaggttttt tcattatcca ttcgctcctt ttatgttagt
     4621 tgtaaaggat tatgcattat ttttcaagta aaggagtctt attaggcgtc cctggttttc
     4681 ttggatactt tttcggcgtc tgtctttttt tatcaataat gaacatctgg cgctctccag
     4741 catcttctgg caattcaaag gtatgtgttt ctgtaacatt accacctaac acactaattg
     4801 caaattttgc ttcttctaat tcttcttcac cttttgaaga ttttaatgca acaaactgtc
     4861 cacctttttt aactagcggt aaacacaatt cacttaacac ggataatcta gctactgctc
     4921 ttgcagtaac aacatcataa gactccctgt agacaccctt accaaatgtt tctgctctat
     4981 cgtgtataaa gctgacatcc tgtaattgta attctgacgc taaatggttt aaaaattgaa
     5041 tacgcttatt taatgaatca acaatcgtca cttttaactg cggaaacatt atttttaacg
     5101 gaatacttgg aaaaccagct ccagcgccta catcacatat acttataggc tgattaaaat
     5161 caaaataaaa actaggtgca atggaatcat aaaaatgttt caaatataca tcgtgttcat
     5221 ctgtaatact tgtcaaattc atcttttcat tccattcaac aagtaaacga taatatgttt
     5281 gaaactgttg tttttgagtc tcagttaatt gaatattatg ttcttttaat tgttctgcta
     5341 accattctac agtcattagt cactcaccct ttggagttta ccttgttcta agtaaatcaa
     5401 taatatagat atgtctgctg gatttacccc tgatattcta gaagcttgtg caatatttaa
     5461 aggttttact tctgataatt tttctcgcgc ttcagtcgcc aaactatcaa tcttactata
     5521 atctaagtct tctggaattt tcttctcttc catacgctta actttctcaa cttgttgtag
     5581 tgatttattg atataacctt catattttgt ttgtatttct acttgttctt caacatctgc
     5641 attcaattga tgttcttctt ctaaaagttc taaaattata tcgtaagtca tttcaggtct
     5701 gcgtaataaa tcgatagcta aaataccatc ttttaagcga gaaccaccat gttgttcaat
     5761 aatcgcttgc gtatgttcgt ttggtttaat acgaatatct gataaacgct taatttccgc
     5821 atcaatttgc tgacgttttt cattaaaacg tgcatatctt tcttcagaaa tcataccaag
     5881 ttcatatccc atatccgtca atctcaaatc agcattatca tgacgtagta acaaacgata
     5941 ttctgcacgt gatgttagta aacgataagg ttcattagta cctttagtta caagatcatc
     6001 gattaagaca ccaatatatg catctgaacg acttaatatc ttttcgcctg tgtttaacac
     6061 tttacctgca gcgttaatac ctgccatcaa tccttgtcct gctgcttctt cataaccaga
     6121 tgtaccatta atttgacctg cagtatataa gtttttaatc attttcgttt caagtgtagg
     6181 ccataactgc gttggcacaa tcgcatcata ttcaattgcg tagccggcac gcatcatatc
     6241 tgctttttca agacctggta tcgtctctaa catttgacgt tgcacatgtt caggaagact
     6301 tgtagacaat ccttgcacat atacttcatt tgtattacga ccttcaggct ctaagaaaag
     6361 ttgatgtcgc ggcttatcat taaatcgaac aaatttatct tcaattgaag ggcaataacg
     6421 tggcccggtt cctttaatca tccctgaata cattgcagat agatgtaaat tatcatcgat
     6481 aactttgtgt gtttcagcat tagtatacgt tagccaacat ggcaattgat ctaatatata
     6541 ttctgttgtt tcaaagctga atgcacgacc tacatcgtca cctggttgta tttcagtctt
     6601 cgaatagtca attgtttttg aatttacacg cggtggtgta cctgttttaa aacgaacaat
     6661 atcaaaacca agttctctta aattgtctga taatgtgatt gatggtaatt ggtgatttgg
     6721 tccacttgaa tacttcatat tacctaaaat gatttcacca cgtaaaaatg ttcccgttgt
     6781 aataattact gctttagata aatactctgt accaatattt gtacgtacac ctttaacttc
     6841 attatcttct ataataagtt cgtctaccat accttgcatt atatgcaaat tttcttcatc
     6901 ttcaatcacg cgtttcattt cttgttgata aagtacttta tctgcttgcg ctcttagtgc
     6961 tcttacagca ggtcctttac ctgtatttaa cattctcatt tgaatgtgtg ttttatcgat
     7021 tgtttttgcc atttgtccac ctaaagcatc aatttcacga acaacgatac ctttagctgg
     7081 tccacctaca gatgggttac atggcataaa tgcaatatta tctaaattta ttgttagcat
     7141 taatgtttta gcaccacgtc ttgcagatgc taaacctgct tctacacctg catgtcccgc
     7201 acctataacg attacatcat attcttgaac cacaatataa acctccttat ttgatatctt
     7261 actagccttc ttaagacggt attccgtcta tttcaattac tatttaccta agcagaattg
     7321 actgaataac tgatcgatga gttcatcact tgcagtctca ccaataattt ctcctaatat
     7381 ttcccaagtt ctagttaaat caatttgtac catatccata ggcacaccag attctgctgc
     7441 atcaatcgca tcttgtatcg tttgtcttgc ttgttttaat aatgaaatat gtcttgaatt
     7501 agaaacataa gtcatatctt gattttgtac ttctccacca aagaacaaat ctcgaatttg
     7561 tatttctaat tcatcaatac cttcttgttt taacattgaa gtttgaatta atggcgtatc
     7621 acctatcata tctttaactt cattaatatc tatgttttgc tctaaatcca ttttattaac
     7681 aattacgatt acatcttcat ttttaaccac ttcatataat gtgtaatctt cttgagtcaa
     7741 tgcttcgtta ttgtttaata caaataaaat taagtctgct tggctaagag cctttctaga
     7801 gcgttcaaca ccaatcttct ctactatatc ttctgtctca cgtataccag cagtatcaac
     7861 taatcttaat ggcacgccac gaacattgac gtactcttct aagacatctc tagtagtacc
     7921 tgctacctca gttacaatcg ctttattatc ttgtattaaa ttatttaaca tcgatgattt
     7981 acctacgttt ggtttaccaa caataactgt agataaacct tcacgcataa ttttaccctg
     8041 cgcaccggta tctaataaac gattaatttc ctgtttgatt tctttagact gctctaaaag
     8101 aaattcagta gtcgcatctt caacatcatc gtattcagga taatcaatat tcacttccac
     8161 ttgagcgagt atctctaata tagattgacg ttgttttttg attaagtcac ttagacgacc
     8221 ttcaatttga ttcatcgcaa ctttagaagc tctatctgtc ttcgagcgaa taaagtccat
     8281 aactgcttca gcttgagata aatcaatacg accatttaaa aaggcacgtt ttgtaaattc
     8341 acctggctca gccattctag cgccatatgt catagtaagt tccagcactc tattaatcgt
     8401 taaaatacca ccatgacaat taatttctat aatatcttcg cgtgtaaatg tttttggcgc
     8461 tcttaacaca gacaccataa cttcttcaat cacttcttta gactctggat caataatatg
     8521 accgtaatta atcgtatgtg atggaacatc atttaaaaga tgttttcctt tatataattt
     8581 gtcagcaatt tcaacggctt gcggtccaga caatcgaaca attccaattg ccccttcacc
     8641 cattggtgtt gaaatactcg taattgtatc taaatccata ttgctactcg cctccttcaa
     8701 cgatgtgaat acattttaaa gtaagttatt ataaccctaa ggtcagtctt acgtttgtct
     8761 gaggtaagac ttcgggatgt gttgagtggt taatgttttc cttcccctac cctatcctta
     8821 cttaatcttt ttattaaaaa ctttggcaat tttaagtacg tgctcaagac tattctgtat
     8881 ttgtaaagtc gtcatatctt tagctggctg tcttgctatt acaataatat ctttggccaa
     8941 tatatgcgac ttatgtactt tgaaattttc acgtattgct cttttaatct tgtttcttaa
     9001 cactgcatta cctagttttt tagaaacact aatacctaag cgaaaatggt ctatttcttt
     9061 attattacaa gtgtatacaa caaattgtct gttggctaca gaatgacctt ttttatatat
     9121 tctctgaaaa tctgcattct ttttaattcg gtaagctttt tccaataaca tcactcgctt
     9181 atttatcgtt tttatttgaa gctatattta aacttctatt gagcttataa cataaatttc
     9241 tatttattct taatttaaac gaaaaaaaag atcactgatg agtcagtgat cttatgcaga
     9301 taaaacttta cggcctttac gacgacggcg cgctaaaact ttacggccat tttttgtgct
     9361 catgcgtttt ctgaaaccat gaactttact atgtttacgt ttatttggtt gataagtacg
     9421 ttttaccatg caaaacacct ccatctttat acagttatct aatataccag attatgcaga
     9481 ctctgatcac tttaatagat aagattttgc gtttcttaga ttaaaattct cgtattagct
     9541 cattgattat ctagtcataa ttcaagcaac tactacaata taacaaaatc ctatttataa
     9601 cgcaagttca ttttatacta ctgctcaatt tttttacttt tatcgattaa agatagaaat
     9661 acacgatgcg agcaatcaaa tttcataaca tcaccatgag tttggtccga agcatgagtg
     9721 tttacaatgt tcgaacacct tatacagttc ttatacatac tttataaatt atttcccaaa
     9781 ctgttttgat acactcacta acagatactc tatagaagga aaagttatcc acttatgcac
     9841 atttatagtt ttcagaattg tggataatta gaaattacac acaaagttat actattttta
     9901 gcaacatatt cacaggtatt tgacatatag aggactgaaa aagtataatt gtgtggataa
     9961 gtcgtccaac tcatgatttt ataaggattt atttattgat ttttacataa aaatactgtg
    10021 cataactaat aagcaagata aagttatcca ccgattgtta ttaacttgtg gataattatt
    10081 aacatggtgt gtttagaagt tatccacggc tgttattttt gtgtataact taaaaattta
    10141 agaaagatgg agtaaattta tgtcggaaaa agaaatttgg gaaaaagtgc ttgaaattgc
    10201 tcaagaaaaa ttatcagctg taagttactc aactttccta aaagatactg agctttacac
    10261 gattaaagat ggtgaagcta tcgtattatc gagtattcct tttaatgcaa attggttaaa
    10321 tcaacaatat gctgaaatta tccaagcaat cttatttgat gttgtaggct atgaagttaa
    10381 acctcacttt attactactg aagaattagc aaattatagt aataatgaaa ctgctactcc
    10441 aaaagaaaca acaaaacctt ctactgaaac aactgaggat aatcatgtgc ttggtagaga
    10501 gcaattcaat gcccataaca catttgacac ttttgtaatc ggacccggta accgctttcc
    10561 acatgcagcg agtttagctg tggccgaagc accagccaaa gcgtacaatc cattatttat
    10621 ctatggaggt gttggtttag gaaaaaccca tttaatgcat gccattggtc atcatgtttt
    10681 agataataat ccagatgcca aagtgattta cacatcaagt gaaaaattca caaatgaatt
    10741 tattaaatca attcgtgata acgaaggtga agctttcaga gaaagatatc gtaatatcga
    10801 cgtcttatta atcgatgata ttcagttcat acaaaacaag gtacaaacac aagaagaatt
    10861 tttctatact tttaatgaat tgcatcagaa taacaagcaa atagttattt cgagtgatcg
    10921 accaccaaag gaaattgcac aattagaaga ccgattacgt tcacgctttg aatgggggct
    10981 aattgttgat attacgccac cagattatga aactcgaatg gcaattttgc agaagaaaat
    11041 tgaagaagaa aaattagata ttccaccaga agctttaaat tatatagcaa atcaaattca
    11101 atctaatatt cgtgaattag aaggtgcatt aacacgttta cttgcatatt cacaattatt
    11161 aggaaaacca attacaactg aattaactgc tgaagcttta aaagatatca ttcaagcacc
    11221 aaaatctaaa aagattacca tccaagatat tcaaaaaatt gtaggccagt actataatgt
    11281 tagaattgaa gatttcagtg caaaaaaacg tacaaagtca attgcatatc cgcgtcaaat
    11341 agctatgtac ttgtctagag agcttacaga tttctcatta cctaaaattg gtgaagaatt
    11401 tggtgggcgt gatcatacga ccgtcattca tgctcatgaa aaaatatcta aagatttaaa
    11461 agaagatcct atttttaaac aagaagtaga gaatcttgaa aaagaaataa gaaatgtata
    11521 agtaggaaac tttgggaaat gtaatctgtt atataacagc actaatgata acaatcattt
    11581 tttacatttc tatatgctaa tgtggcaaga tgagcaaaac tcattttgtg gataatgttt
    11641 aaaagtcata cacaccatac acaagttatc aacatgtgta taacttcgcc aaatctatgt
    11701 ttttaagact tatccaccaa tccacagcac ctactactat tactaagaac ttaaaaccta
    11761 tataattata tataaacgac tggaaggagt tttaattaat gatggaattc actattaaaa
    11821 gagattattt tattacacaa ttaaatgaca cattaaaagc tatttcacca agaacaacat
    11881 tacctatatt aactggtatc aaaatcgatg cgaaagaaca tgaagttata ttaactggtt
    11941 cagactctga aatttcaata gaaatcacta ttcctaaaac tgtagatggc gaagatattg
    12001 tcaatatttc agaaacaggc tcagtagtac ttcctggacg attctttgtt gatattataa
    12061 aaaaattacc tggtaaagat gttaaattat ctacaaatga acaattccag acattaatta
    12121 catcaggtca ttctgaattt aatttaagtg gcttagatcc agatcaatat cctttattac
    12181 ctcaagtttc tagagatgac gcaattcaat tgtcggtaaa agtgcttaaa aacgtgattg
    12241 cacaaacaaa ttttgcagtg tccacctcag aaacacgccc agtactaact ggtgtgaact
    12301 ggcttataca agaaaatgaa ttaatatgca cagcgactga ctcacaccgc ttggctgtaa
    12361 gaaagttgca gttagaagat gtttctgaaa acaaaaatgt catcattcca ggtaaggctt
    12421 tagctgaatt aaataaaatt atgtctgaca atgaagaaga cattgatatc ttctttgctt
    12481 caaaccaagt tttatttaaa gttggaaatg tgaactttat ttctcgatta ttagaaggac
    12541 attatcctga tacaacacgt ttattccctg aaaactatga aattaaatta agtatagaca
    12601 atggggagtt ttatcatgcg attgatcgtg cctctttatt agcgcgtgaa ggtggtaata
    12661 acgttattaa attaagtaca ggtgatgacg ttgttgaatt gtcttctaca tcaccagaaa
    12721 ttggtactgt aaaagaagaa gttgatgcaa acgatgttga aggtggtagc ctgaaaattt
    12781 cattcaactc taaatatatg atggatgctt taaaagcaat cgataatgat gaggttgaag
    12841 ttgaattctt cggtacaatg aaaccattta ttctaaaacc aaaaggtgac gactcggtaa
    12901 cgcaattaat tttaccaatc agaacttact aaaaataaat ataaataaag gatgacgtga
    12961 ttaattaaaa cgtcatcctt tattttttgg caaaaataat tctaggtgcg tatgtaaaat
    13021 aaatttggca gcattttaaa cagcaaataa aagacgccaa ttaaatttat gacaaatgta
    13081 tccaaaattt aataagtgtg cttatatgcc ctttaaattt aaaattttaa tagtcaataa
    13141 caagttgaat ataaaagtta aacgccgtta aatagcgtta aaaaattgaa aatgacagta
    13201 ttgccaaaaa ataagaatta attatttata tgtaaacggt ttctacctct attttaaatg
    13261 aaatttgtga caaaaaaagg tataatatat taatgacata caaagaaatg gagtgattat
    13321 tttggttcaa gaagttgtag tagaaggaga cattaattta ggtcaatttc taaaaacaga
    13381 agggattatt gaatctggtg gtcaagcaaa atggttcttg caagacgttg aagtattaat
    13441 taatggagtg cgtgaaacac gtcgcggtaa aaagttagaa catcaagatc gtatagatat
    13501 cccagaatta cctgaagatg ctggttcttt cttaatcatt catcaaggtg aacaatgaag
    13561 ttaaatacac tccaattaga aaattatcgt aactatgatg aggttacgtt gaaatgtcat
    13621 cctgacgtga atatcctcat tggagaaaat gcacaaggaa agacaaattt acttgaatca
    13681 atttatacct tagctttagc aaaaagtcat agaacgagta atgataagga actcatacgt
    13741 tttaatgctg attatgctaa aatagaaggt gagcttagtt atagacacgg cacgatgcca
    13801 ttaacaatgt ttataactaa aaaaggtaaa caagtcaaag tgaatcactt agagcaaagt
    13861 cgtctaactc aatatattgg acacctcaat gtggttctat ttgcgccaga agatttgaat
    13921 attgtaaaag gctctcctca aataagacga cgctttatag atatggagtt gggccaaatt
    13981 tctgctgttt acttaaatga tttagctcaa taccaacgta ttttaaagca aaagaataat
    14041 tacttaaagc agttacaatt aggccaaaaa aaggacttaa caatgttgga agtattaaat
    14101 cagcagtttg ctgaatatgc aatgaaagta actgataaac gtgcacattt tattcaagag
    14161 ctagagtcgt tagctaaacc gattcatgct ggtatcacaa atgataaaga agcgttgtcg
    14221 ctgaattatt tacctagtct taaatttgat tatgctcaaa atgaagcggc acgacttgaa
    14281 gaaattatgt ctattcttag cgataatatg caaagagaaa aagaacgagg cattagctta
    14341 ttcggaccac atcgagatga tataagtttt gatgtgaatg gcatggatgc tcaaacatat
    14401 ggttctcaag gacagcaacg tacaacggct ttgtccatta aattagctga aattgagtta
    14461 atgaatatcg aagttgggga atatcccatc ttattattag acgatgtact cagtgaatta
    14521 gatgattcgc gtcaaacgca tttattaagt acgattcagc ataaagtaca aacatttgtc
    14581 actacgacat ctgtagatgg tattgatcat gaaatcatga ataacgctaa attgtatcgt
    14641 attaatcaag gtgaaattat aaagtaacag aaagcgatgg tgactgcatt gtcagatgta
    14701 aacaacacgg ataattatgg tgctgggcaa atacaagtat tagaaggttt agaagcagta
    14761 cgtaaaagac caggtatgta tataggatcg acttcagaga gaggtttgca ccatttagtg
    14821 tgggaaattg tcgataatag tatcgatgaa gcattagctg gttatgcaaa tcaaattgaa
    14881 gttgttattg aaaaagataa ctggattaaa gtaacggata acggacgtgg tatcccagtt
    14941 gatattcaag aaaaaatggg acgtccagct gtcgaagtta ttttaactgt tttacatgct
    15001 ggtggtaaat ttggcggtgg cggatacaaa gtatctggtg gtttacatgg tgttggttca
    15061 tcagttgtaa acgcattgtc acaagactta gaagtatatg tacacagaaa tgagactata
    15121 tatcatcaag catataaaaa aggtgtacct caatttgact taaaagaagt tggcacaact
    15181 gataagacag gtactgtcat tcgttttaaa gcagatggag aaatcttcac agagacaact
    15241 gtatacaact atgaaacatt acagcagcgt attagagagc ttgctttctt aaacaaagga
    15301 attcaaatca cattaagaga tgaacgtgat gaagaaaacg ttagagaaga ctcctatcac
    15361 tatgagggcg gtattaaatc gtacgttgag ttattgaacg aaaataaaga acctattcat
    15421 gatgagccaa tttatattca tcaatctaaa gatgatattg aagtagaaat tgcgattcaa
    15481 tataactcag gatatgccac aaatctttta acttacgcaa ataacattca tacgtatgaa
    15541 ggtggtacgc atgaagacgg attcaaacgt gcattaacgc gtgtcttaaa tagttatggt
    15601 ttaagtagca agattatgaa agaagaaaaa gatagacttt ctggtgaaga tacacgtgaa
    15661 ggtatgacag caattatatc tatcaaacat ggtgatcctc aattcgaagg tcaaacgaag
    15721 acaaaattag gtaattctga agtgcgtcaa gttgtagata aattattctc agagcacttt
    15781 gaacgatttt tatatgaaaa tccacaagtc gcacgtacag tggttgaaaa aggtattatg
    15841 gcggcacgtg cacgtgttgc tgcgaaaaaa gcgcgtgaag taacacgtcg taaatcagcg
    15901 ttagatgtag caagccttcc aggtaaatta gccgattgct ctagtaaaag tcctgaagaa
    15961 tgtgagattt tcttagtcga aggggactct gccggggggt ctacaaaatc tggtcgtgac
    16021 tctagaacgc aggcgatttt accattacga ggtaagatat taaatgttga aaaagcacga
    16081 ttagatagaa ttttgaataa caatgaaatt cgtcaaatga tcacagcatt tggtacagga
    16141 atcggtggcg actttgatct agcgaaagca agatatcaca aaatcgtcat tatgactgat
    16201 gccgatgtgg atggagcgca tattagaaca ttgttattaa cattcttcta tcgatttatg
    16261 agaccgttaa ttgaagcagg ctatgtgtat attgcacagc caccgttgta taaactgaca
    16321 caaggtaaac aaaagtatta tgtatacaat gatagggaac ttgataaact taaatctgaa
    16381 ttgaatccaa caccaaaatg gtctattgca cgatacaaag gtcttggaga aatgaatgca
    16441 gatcaattat gggaaacaac aatgaaccct gagcaccgcg ctcttttaca agtaaaactt
    16501 gaagatgcga ttgaagcgga ccaaacattt gaaatgttaa tgggtgacgt tgtagaaaac
    16561 cgtagacaat ttatagaaga taatgcagtt tatgcaaact tagacttcta agcgctgtga
    16621 actgaacttt tgaaggagga actcttgatg gctgaattac ctcaatcaag aataaatgaa
    16681 cgaaatatta ccagtgaaat gcgtgaatca tttttagatt atgcgatgag tgttatcgtt
    16741 gctcgtgcat tgccagatgt tcgtgacggt ttaaaaccag tacatcgtcg tatactatat
    16801 ggattaaatg aacaaggtat gacaccggat aaatcatata aaaaatcagc acgtatcgtt
    16861 ggtgacgtaa tgggtaaata tcaccctcat ggtgactcat ctatttatga agcaatggta
    16921 cgtatggctc aagatttcag ttatcgttat ccgcttgttg atggccaagg taactttggt
    16981 tcaatggatg gagatggcgc agcagcaatg cgttatactg aagcgcgtat gactaaaatc
    17041 acacttgaac tgttacgtga tattaataaa gatacaatag attttatcga taactatgat
    17101 ggtaatgaaa gagagccgtc agtcttacct gctcgattcc ctaacttatt agccaatggt
    17161 gcatcaggta tcgcggtagg tatggcaacg aatattccac cacataactt aacagaatta
    17221 atcaatggtg tacttagctt aagtaagaac cctgatattt caattgctga gttaatggag
    17281 gatattgaag gtcctgattt cccaactgct ggacttattt taggtaagag tggtattaga
    17341 cgtgcatatg aaacaggtcg tggttcaatt caaatgcgtt ctcgtgcagt tattgaagaa
    17401 cgtggaggcg gacgtcaacg tattgttgtc actgaaattc ctttccaagt gaataaggct
    17461 cgtatgattg aaaaaattgc agagctcgtt cgtgacaaga aaattgacgg tatcactgat
    17521 ttacgtgatg aaacaagttt acgtactggt gtgcgtgtcg ttattgatgt gcgtaaggat
    17581 gcaaatgcta gtgtcatttt aaataactta tacaaacaaa cacctcttca aacatcattt
    17641 ggtgtgaata tgattgcact tgtaaatggt agaccgaagc ttattaattt aaaagaagcg
    17701 ttggtacatt atttagagca tcaaaagaca gttgttagaa gacgtacgca atacaactta
    17761 cgtaaagcta aagatcgtgc ccacatttta gaaggattac gtatcgcact tgaccatatc
    17821 gatgaaatta tttcaacgat tcgtgagtca gatacagata aagttgcaat ggaaagcttg
    17881 caacaacgct tcaaactttc tgaaaaacaa gctcaagcta ttttagacat gcgtttaaga
    17941 cgtctaacag gtttagagag agacaaaatt gaagctgaat ataatgagtt attaaattat
    18001 attagtgaat tagaagcaat cttagctgat gaagaagtgt tattacagtt agttagagat
    18061 gaattgactg aaattagaga tcgtttcggt gatgatcgtc gtacagaaat tcaattaggt
    18121 ggatttgaaa acttagagga cgaagactta attccagaag aacaaatagt aattacacta
    18181 agccataata actacattaa acgtttgccg gtatctacat atcgtgctca aaaccgtggt
    18241 ggtcgtggtg ttcaaggtat gaatacattg gaagaagatt ttgtcagtca attggtaact
    18301 ttaagtacac atgaccatgt attgttcttt actaacaaag gtcgtgtata caaacttaaa
    18361 ggttacgaag tgcctgagtt atcaagacag tctaaaggta ttcctgtagt gaatgctatt
    18421 gaacttgaaa atgatgaagt cattagtaca atgattgctg ttaaagacct tgaaagtgaa
    18481 gacaacttct tagtgtttgc aactaaacgt ggtgtcgtta aacgttcagc attaagtaac
    18541 ttctcaagaa taaatagaaa tggtaagatt gcgatttcgt tcagagaaga tgatgagtta
    18601 attgcagttc gcttaacaag tggtcaagaa gatatcttga ttggtacatc acatgcatca
    18661 ttaattcgat tccctgaatc aacattacgt cctttaggcc gtacagcaac gggtgtgaaa
    18721 ggtattacac ttcgtgaagg tgacgaagtt gtagggcttg atgtagctca tgcaaacagt
    18781 gttgatgaag tattagtagt tactgaaaat ggttatggta aacgtacgcc agttaatgac
    18841 tatcgcttat caaatcgtgg tggtaaaggt attaaaacag ctacgattac tgagcgtaat
    18901 ggtaatgttg tatgtatcac tacagtaact ggtgaagaag atttaatgat tgttactaat
    18961 gcaggtgtca ttattcgact agatgttgca gatatttctc aaaatggtcg tgcagcacaa
    19021 ggtgttcgct taattcgctt aggtgatgat caatttgttt caacggttgc taaagtaaaa
    19081 gaagatgcag aagatgaaac gaatgaagat gagcaatcta cttcaactgt atctgaagat
    19141 ggtactgaac aacaacgtga agcggttgta aatgatgaaa caccaggaaa tgcaattcat
    19201 actgaagtga ttgattcaga agaaaatgat gaagatggac gtattgaagt aagacaagat
    19261 ttcatggatc gtgttgaaga agatatacaa caatcatcag atgaagaata ataaaaaata
    19321 agacttccct atatgtaggg gagtcttatt tttatgctag aaagtaatgc tttactatat
    19381 tcaatgatta gtaatgacta actttctaat tgtttcattg cgtaaggtat ttcattgata
    19441 agtcttgatg gtggcaccac atacatatct tttgcaaggt tttcgccaat aaaactatgt
    19501 gtatatgtgg cactcataac cgcttctttt aagttatcaa attgaccgac aaagcttgta
    19561 atcataccag caagtgtatc gcccatacca ccagtcgcca ttgctgggct accgattgtc
    19621 aatttaaagt cttcatcttt aaagaaaatt tcagtaccat gttttttaag tacaacagtt
    19681 gcacctaaac gatcaactgc ttcacgatta cgctcatatg tctgttcctc aataggaata
    19741 ccacttaatc gttcccattc tttgaggtgt ggtgtaaaga tcacacgaca tgtaggtaat
    19801 tgcggtttca gtttactaaa gattgtaatc gcatcgccgt ctacgattaa attttgatgc
    19861 ggttgtatat tttgtagtag gaatgtaatg gcattatttc ctttgaaatc aacgccaaga
    19921 cctggaccaa ttagtatact gtcagtcatt tcaatcattt tcgtcaacat tttcgtatca
    19981 ttaatatcaa taaccatcgc ttctgggcaa cgagaatgta atgctgaatg atttgttgga
    20041 tgtgtagcta cagtgattaa accactaccg ctaaatacac atgcacgagc cgctaacata
    20101 atggcaccac ctaagttagc agatccacca attaataaaa ttttgccata atcaccttta
    20161 tgtgaatctt ctttacgctt aggaatgtta atagaattta acgtttccat agtgatataa
    20221 cctcccatgt aaaagccttt tccgaattta ttcaatttta aaaatatata gtaactttta
    20281 acaaaatgta ttataaattt ctgaattcat tattatttgt cgttaaatac aatagaaaat
    20341 actatacctg tatatgcaat tcgtcaatag ataaattatt aaatatgctt acaacaatct
    20401 taatatcctt taacgcacta caatagtgct ctgataatag gttataaatg tacgtaaaac
    20461 cattgtttca ataaaaatga aaacgtatac ttcaagaagg atgggttact taatataaac
    20521 aagggggtaa catatatgac tttatattta gatggtgaaa cactaacaat tgaggatatt
    20581 aaatcatttt tacaacaaca atcaaagatt gaaattattg atgatgcgtt agaacgtgtc
    20641 aaaaaaagta gagcggtagt tgaacgtatt attgaaaatg aggaaacggt ttacggtatt
    20701 actacaggtt ttgggttatt tagtgatgta cgtatagacc cgacgcaata taatgaatta
    20761 caagtgaatc tgatacgctc acatgcctgt ggactaggtg agccattttc aaaagaagta
    20821 gcattagtca tgatgatttt acgattgaat acattattaa aaggtcattc aggtgccact
    20881 ttagaattag tgagacaatt acaatttttt ataaatgaac gtattatacc gataatccca
    20941 cagcaaggct ctctcggtgc atcaggagat ttagcgccat tatcacattt agcattagca
    21001 ttaattggtg aagggaaagt attgtacaga ggggaagaaa aggatagtga cgatgtatta
    21061 agagaattaa atagacaacc tttgaacctt caggctaaag aaggtttagc attgattaat
    21121 ggtacgcaag ctatgacagc tcaaggtgtc attagttata tagaagcaga agatttaggt
    21181 taccaatctg aatggattgc tgcattaacg catcagtctc ttaatggcat tatagatgca
    21241 tatcgacatg atgtgcacgc agttcgtaat tttcaagaac agattaatgt ggcagcgcgt
    21301 atgcgtgatt ggttagaagg atcaacatta acgacgcgac aatcagaaat acgtgtacaa
    21361 gatgcatata cgttgcgttg tataccacaa atccatggcg cgagttttca agtattcaat
    21421 tatgttaaac agcaattaga atttgaaatg aatgcggcta atgataatcc acttatattt
    21481 gaggaagcaa atgaaacgtt tgttatttca ggtggtaact tccatggaca acctattgct
    21541 tttgcattag atcatcttaa attaggtgta agtgaattag caaacgtatc ggaacgtcgt
    21601 ctagagcgac tagtaaatcc tcaattaaat ggtgatttac cagcatttct tagtccagag
    21661 ccaggattgc aaagtggcgc gatgattatg caatatgctg ctgcaagtct cgtttctgaa
    21721 aataaaactt tagcgcatcc agcgagtgtt gattctatca cttcatctgc gaaccaagaa
    21781 gatcacgtat ctatgggaac tacagctgct agacatggtt atcaaattat tgaaaatgca
    21841 agacgtgtgc tggcaatcga atgtgttatt gcattacaag cagcagagtt aaaaggtgtt
    21901 gaaggattat caccaaaaac acgtcgcaag tatgatgagt ttcgaagtat cgtgccatcc
    21961 attacacatg atcgtcaatt tcataaagat attgaagcgg ttgcacagta tttaaagcaa
    22021 tcaatttatc aaacgactgc atgtcactaa atcgacatga gtgatgtttg aaatcgttac
    22081 ttgaaaaagc aaatatagtt tgctatatta aaaataactt aataagacat tgttcgtagg
    22141 acaagtaata tatagtgttc gatatcagag agcttgtggt tagtgtgaac aagaatcaac
    22201 atatatatga atctacctac ttaatttaaa agaacaatcg gtgataaccg ttattttagt
    22261 gaagtgcaat ttaggtttag tgtatcttta taacttaaat tgttaaatag ggtggcaacg
    22321 cgtagaccac gtcccttgta gggatgtggt ctttttttat tttctaaaaa ataaagacgt
    22381 acgtcattcc ataataaatg ataattattt gggaaaggat gaaggttaat gttagacatt
    22441 agattattca gaaatgagcc tgacacagtt aagagcaaaa ttgaattacg tggagatgat
    22501 ccaaaagttg tagatgaaat tttagaattg gatgagcaac gacgtaaatt aattagtgca
    22561 acagaagaaa tgaaagcacg tcgtaataaa gtaagcgaag aaatcgcatt aaaaaaacgt
    22621 aataaagaaa atgctgatga tgtgattgct gaaatgcgca cattaggtga cgatattaaa
    22681 gaaaaagata gtcaattaaa tgaaattgat aataaaatga caggtatcct ttgtcgtatt
    22741 ccaaatttaa taagtgatga tgtacctcaa ggtgaatctg atgaagataa cgttgaagtt
    22801 aaaaagtggg gtacaccacg tgagttttca tttgaaccca aagcacattg ggatattgta
    22861 gaagaattga aaatggctga ttttgatcgt gcagcaaaag tttcaggtgc gcgttttgta
    22921 tatttaacaa atgaaggtgc gcaattagag cgtgctttaa tgaactatat gattacaaaa
    22981 catacaacac aacatggtta tacagaaatg atggtaccac agcttgtgaa cgcagataca
    23041 atgtatggta caggtcaatt acctaaattt gaagaagatt tatttaaagt agaaaaagaa
    23101 ggattatata caattccaac tgctgaagta ccattaacga atttctaccg taatgaaatt
    23161 attcaaccag gtgtacttcc tgaaaaattc actggtcaat ctgcatgttt ccgtagtgaa
    23221 gcaggatcag caggtagaga tacaagagga ttaattcgtt tacatcaatt cgataaagtg
    23281 gaaatggtac gttttgaaca acctgaagat tcatggaatg ctttagaaga aatgacaaca
    23341 aacgcagaag caattctaga agagttaggt ttaccatacc gtcgtgttat tttatgtaca
    23401 ggtgatattg gatttagtgc aagcaaaaca tatgatttag aagtttggtt accaagctac
    23461 aatgattata aagaaattag ttcatgctca aactgtacgg atttccaagc gcgtcgtgct
    23521 aacatccgct tcaagcgtga caaagcagct aaaccagaat tagcacatac attaaatggt
    23581 agtggtttag cagttggacg tacatttgct gctattgttg aaaattacca aaatgaagat
    23641 ggaacagtaa caattccaga agcattagta ccatttatgg gtggtaaaac acaaatttca
    23701 aaaccagtta aataaaggct ttagctacaa gctttaaaaa gtatatatct acgtatactt
    23761 aaagcaaggg caagatactt taaataatat tttaaaaagt ggtgacgaag ctgtcgccac
    23821 tttttttgtg ctgtaaaagt ataatagtga gccaatgcac ataacaacaa taaattaagt
    23881 ttgtggttta atggggtgaa cgcatttcat tatagcaaca atacgggata attatgatga
    23941 actaaaacaa tctaaaacgt aacaagtttg agcatcacca atataggaaa ggaagcgata
    24001 aaatactgat ttcgttgata tgtagtatga gttatatcga tggagtaggg tagggggagg
    24061 ggatgattat aagggagtgg tacatgaatc aatatcccag actcatcatc agatataaaa
    24121 atttataaaa ttgatactta aaaacaacta caaatccata gaaaatatgg aggtagtctt
    24181 aaataaaaaa ttgaaaattc tcaaaaataa aaagttaata tgaagctgac taaagactct
    24241 ggtatgtcta acctcagaca aactgatgtc taatgttatt gcttagggta tagaactgta
    24301 ttagactagg tatattattt tttcgtaatt atataaatat aaagtggcaa aggaggtaat
    24361 tgagatgaca acacatttaa gttttagaca aggcgtgcaa gagtgtatcc caacattatt
    24421 gggttatgcc ggtgttggta tttcatttgg tattgtggct tcgtctcaaa actttagtat
    24481 tttagaaatt gtcttgttat gtcttgttat atatgccggt gctgcgcaat ttattatgtg
    24541 cgcgttgttt atagcaggta cacctatatc agcgattgta ctaactgtat ttatcgtaaa
    24601 ttcaagaatg ttccttttaa gtatgtcgct tgcaccaaac ttcaagacat atgggttttg
    24661 gaaccgtgtt ggattaggtt cattagtaac tgacgaaacg tttggcgtcg ccattacacc
    24721 ttatttaaaa ggagaagcta tcaatgatcg ttggatgcat ggtcttaaca tcacagcata
    24781 tttattttgg gcaatttcat gtgtagctgg ggctttattt ggcgaatata tctcaaatcc
    24841 gcaaacgcta gggttagatt ttgctatcac ggctatgttt atctttttgg ccattgcgca
    24901 atttgaatca attactaaat cgcgattaag aatttacata gtactcatta ttgccgtcat
    24961 agtaatgatg ttatcgctaa gtatgtttat gccttcatat ctagcaatat taattgcagc
    25021 cacaatttca gcagcgttag gagtgatgat ggaacaatga taactcatat gaacatgtta
    25081 atacttattt tattgtgtgg tatcgtaacg ctattaattc gaattatacc ttttatcatg
    25141 atttcaaaag tgcaattgcc tgatgtcgtg gttcgatggc tatcatttat cccaatcaca
    25201 ctatttacgg cacttgtcat tgacagcatt attcaacaga cgcctcatgg tgaggggtat
    25261 acattaaaca tcccttacat tatcgcgctc attccgacgg ttattttatc tataatcacg
    25321 cgtagtttaa ctattacaat tattagtggg attgttatca tggcagcatt acgatttttc
    25381 ttttaaaaat aactgaaaat cattgaacag atatttaaac ttaggtaaac tgttagtaat
    25441 cagaaaattc tgatatacaa atcgtctatg ttaagattgg gaatcgtgta tacgtaagcg
    25501 atttggctaa cgcatgacaa tgataacaac attttaaatg ataaaagtaa ttcatcactg
    25561 aatctcaact aacacataac aatttcatat ttcttattgt gagaagttga gggacttggc
    25621 cctgtgatac ttcagcaacc gactttatag cacggtgcta aaaccaacga gttactcgaa
    25681 tgataagtat aaagacttct tacttttcaa tagggtgaga agtttttttg tttaaggagg
    25741 aaagaacaat gacaaattac acagtagata ctttaaattt aggggaattt attacagaat
    25801 ctggggaagt catagataac ttgcgtttga gatatgagca tgtcggttat catggacaac
    25861 cattagttgt agtttgtcat gcattaactg gcaatcattt aacatatgga acagatgatt
    25921 atccgggttg gtggcgagaa attattgatg ggggatatat acccattcac gattatcaat
    25981 ttttaacatt tgatgttatt ggtagtcctt tcggttcaag ttcaccttta aacgaccctc
    26041 attttcctaa aaaattaaca ttaagagata tagtcagagc gaatgaacga ggtatacaag
    26101 cccttggtta tgataagatt aatattttaa tagggggaag tcttggaggt atgcaagcaa
    26161 tggaactact ctacaatcaa cagtttgaag tagataaagc cattatcctt gctgcaacaa
    26221 gtcgaacatc atcttatagt agagctttca atgaaattgc aaggcaagcc attcatcttg
    26281 gtggtaagga aggtctaagt attgcacgcc aattaggttt tttgacatat cgatcatcaa
    26341 aaagttatga tgaacgtttc acgccggatg aagtagtcgc ataccaacaa catcaaggta
    26401 ataaatttaa agaacatttt gatttgaatt gttatctgac actgctagat gtattggata
    26461 gtcacaacat tgaccgaggt cgcacagacg taacgcatgt ttttaaaaat ttagaaacaa
    26521 aagtgttaac gatggggttc atagatgatt tgctatatcc ggatgatcaa gttcgtgcat
    26581 taggtgaacg ttttaaatat catcgtcatt tcttcgtgcc tgataatgtt gggcatgatg
    26641 gattcctact aaactttagt acctgggcac ctaacttata tcatttctta aatttaaagc
    26701 attttaagcg taagtaataa tatatatttt caaagaatga agccactaaa tcacagttat
    26761 catattcaaa aacatctaga gtacatgaga gtggaaatgt attgtaaatc taaatcaagc
    26821 ttgattgcta tgtagtggga ttaaataatc atgttgaaaa tgaaagcaca caaaagcgat
    26881 tcaaatgagt atgttaatta cttataaaac atgaaagtta ttattcaatt aaatgaaata
    26941 gaagcataca atctataaat tgttaatttt cattaaagag gttaaaataa tagctatagt
    27001 taaaaatatg ggagtggctt atgtgttttc aaaaatacaa cctaaagcaa caataattgc
    27061 aacgattgcg ttggtatttg tcgctttagc tttatatcta gtgcctggtt taggactaat
    27121 atttgcatta tttgcaacca taccaggtat cgttttatgg aataaatcaa tacaatcttt
    27181 cgggattagt gcacttatta cagtaattat aacaactgtt ttaggtaata ctttcgtttt
    27241 aagtgccatc atattagtct taattgcaag tttaattatt ggtcaattgc tcaaagaaag
    27301 aacgtctaaa gaaagaatat tatacgtaac aacagtagcg atgagcttaa tttcattaat
    27361 cgcttttatg ttactacaaa cattcggaag gattccacca tcagcgagca tagtaaaacc
    27421 tttcaagcaa acattacatg aagcgattac gatgagcggt gccgatgcga atatgaccca
    27481 aatattagaa gaagggttta gacaagcgac cgttcaatta ccaggtttca tcattatcat
    27541 tacattttta atcgtcttaa ttaacttaat cgttacattt ccgattttac gaaaatttaa
    27601 aatcgctaca cctgtattta agccactttt cgcgtggcaa atgagcggta ttttattatg
    27661 gatatacatt attgttatca tatgtttatt atttacaggt caaccgagtg tgttccagag
    27721 cattctttta aacttccaac ttgtgttatc attagtaatg tatattcaag gtttaagtgt
    27781 tattcatttc tttggtaaag cgaaaggttt gccgaatgca gtaacgattt tactattggt
    27841 tatcggtaca atactgacac ctacgacaca tattgtagga ctacttggtg ttatcgattt
    27901 aagtttgaat ttgaagcgaa tcatgaaaaa taattctaaa aagtgaatag aggtggaata
    27961 atgaatcggc agtccactaa gaaagcttta ctaataccat ttgtcatcat gatcatcaca
    28021 gcaattgttt taatgggtgt atggtttatc tttaatagtc ttatagcact aattgcatct
    28081 atcgttcttg tcgtgatgat tattgttagc atcattttat tcagacaagc tttaatgaaa
    28141 atggatagtt atgtagatgg tttgagtgct caaatttcaa caacaaataa taaagcaatc
    28201 aaacatttac caattggtat cattgtttta gatgaaaatg atcacatcga atgggttaac
    28261 caatttatga cagatcatat ggaagcgaat gtcatttctg aatctgtaaa tgaagtattt
    28321 ccaaacattt taaagcaatt agatagagtg aaatccgttg aaatagaata taatcagtat
    28381 catttccaag tacgttattc tgagaatgat cactgcctct atttctttga tataactgaa
    28441 caagtacaaa caaatgaact atatgaaaat tctaaaccaa tcattgcgac attattttta
    28501 gataactacg atgagattac gcaaaatatg aatgatacgc agcgttcgga aatcaactca
    28561 atggtaacgc gtgtcattag tcgatgggca actgagtata atatattttt caaaagatac
    28621 agttccgatc aattcgtagc ctatttaaat caaaaaatat tagctgactt agaagaatct
    28681 aaatttgata tcttgagtca attacgtgaa aaaagtgttg gttatcgtgc ccaattaaca
    28741 ttaagtatcg gtgttggtga aggtactgaa aatttaatcg acttaggtga attatcacaa
    28801 tcaggcctag acttagcatt aggacgcggt ggcgaccaag ttgcaattaa aagtattaat
    28861 ggtaatgtgc gtttctatgg cggtaagact gacccgatgg agaaacgtac tcgtgtaaga
    28921 gcacgtgtga tctcacatgc gttaaaagat atccttgcag agggtgacaa agtcattatc
    28981 atgggacata aacgtcctga cttagatgca attggtgcag caatcggtgt gtctagattt
    29041 gcaatgatga ataatttaga agcatacatc gtattaaatg agactgacat tgatccaaca
    29101 ttacgacgcg tgatgaacga aatagataaa aagccagagt taagagagcg atttattaca
    29161 tcagatgatg cttgggatat gatgacatct aagacaaccg tagtgattgt tgatacgcat
    29221 aaaccggaac tggttttaga tgaaaatgtc ttaaataaag caaaccgtaa agttgttatc
    29281 gatcatcata gacgtggtga aagcttcatc tctaatccat tgttgatata tatggaacca
    29341 tacgcaagtt cgacagctga attggtaaca gagttactgg aatatcaacc aacagaacaa
    29401 cgtttaacac gtcttgaatc aacagtgatg tatgcaggta ttattgtaga tacaagaaac
    29461 tttacattac gaacaggatc aagaacattc gatgcagcga gttatttacg tgcacatggt
    29521 gcagatacga ttttaacgca acatttctta aaagatgatg tggatactta cattaatcga
    29581 tctgaattaa ttcgaactgt aaaagttgaa gataatggca tagccattgc gcatggttca
    29641 gacgataaaa tttatcatcc agtaacagtt gcacaagcag cagatgaact gttaagttta
    29701 gaaggtattg aagcatcata tgttgttgcg agacgtgaag ataatctgat tggtatatct
    29761 gcgcgttcac tcggttcagt aaatgtccag ttaacaatgg aagcacttgg tggcggtgga
    29821 catttaacca atgcggcaac acaacttaaa ggtgtgacag tcgaagaggc gatagcacaa
    29881 ttacaacaag caattacaga acaattaagt aggagtgaag atgcatgaaa gtaattttta
    29941 cacaagatgt taaaggtaaa ggtaaaaaag gtgaagttaa agaagtacca gtaggttatg
    30001 caaataactt cttattgaaa aagaattatg ctgtagaagc aacaccaggt aaccttaaac
    30061 aattagagtt acagaaaaaa cgtgcaaaac aagaacgcca acaagaaatt gaagatgcta
    30121 aagcattaaa agaaacgtta tcaaacattg aagttgaagt atcagcaaaa actggtgaag
    30181 gtggtaaatt gtttgggtca gtaagtacaa aacaaattgc cgaagcacta aaagcacaac
    30241 atgatattaa aattgataaa cgtaaaatgg atttaccaaa tggaattcat tccctaggat
    30301 atacgaatgt acctgttaaa ttagataaag aagttgaagg tacaattcgc gtacacacag
    30361 ttgaacaata aagttggatt gaaataagag gtgtaaccat tcatggatag aatgtatgag
    30421 caaaatcaaa tgccgcataa caatgaagct gaacagtctg tcttaggttc aattattata
    30481 gatccagaat tgattaatac tactcaggaa gttttgcttc ctgagtcgtt ttataggggt
    30541 gcccatcaac atattttccg tgcaatgatg cacttaaatg aagataataa agaaattgat
    30601 gttgtaacat tgatggatca attatcgacg gaaggtacgt tgaatgaagc gggtggcccg
    30661 caatatcttg cagagttatc tacaaatgta ccaacgacgc gaaatgttca gtattatact
    30721 gatatcgttt ctaagcatgc attaaaacgt agattgattc aaactgcaga tagtattgcc
    30781 aatgatggat ataatgatga acttgaacta gatgcgattt taagtgatgc agaacgtcga
    30841 attttagagc tatcatcttc tcgtgaaagc gatggcttta aagacattcg agacgtctta
    30901 ggacaagtgt atgaaacagc tgaagagctt gatcaaaata gtggtcaaac accaggtata
    30961 cctacaggat atcgagattt agaccaaatg acagcagggt tcaaccgaaa tgatttaatt
    31021 atccttgcag cgcgtccatc tgtaggtaag actgcgttcg cacttaatat tgcacaaaaa
    31081 gttgcaacgc atgaagatat gtatacagtt ggtattttct cgctagagat gggtgctgat
    31141 cagttagcca cacgtatgat ttgtagttct ggaaatgttg actcaaaccg cttaagaacg
    31201 ggtactatga ctgaggaaga ttggagtcgt tttactatag cggtaggtaa attatcacgt
    31261 acgaagattt ttattgatga tacaccgggt attcgaatta atgatttacg ttctaaatgt
    31321 cgtcgattaa agcaagaaca tggcttagac atgattgtga ttgactactt acagttgatt
    31381 caaggtagtg gttcacgtgc gtccgataac agacaacagg aagtttctga aatctctcgt
    31441 acattaaaag cattagcccg tgaattagaa tgtccagtta tcgcattaag tcagttatct
    31501 cgtggtgttg aacaacgaca agataaacgt ccaatgatga gtgatattcg tgaatctggt
    31561 tcgattgagc aagatgccga tatcgttgca ttcttatacc gtgatgatta ctataaccgt
    31621 ggcggcgatg aagatgatga cgatgatggt ggtttcgagc cacaaacgaa tgatgaaaac
    31681 ggtgaaattg aaattatcat tgctaagcaa cgtaacggtc caacaggcac agttaagtta
    31741 cattttatga aacaatataa taaatttacc gatatcgatt atgcacatgc agatatgatg
    31801 taaaaaagtt tttccgtaca ataatcatta agatgataaa attgtacggt ttttattttg
    31861 ttctgaacgg gttgatatat gttaagtttg tgtattgaaa gtgataaatt agtactgtca
    31921 acgcctctgt taaagggttt ttaggacgtt gaaaacgatt tgttaaaatg atttttcttt
    31981 taaaaaggcc gaaaatcaat gttcgatttt tatttgcatt atggtctcga tattggtaga
    32041 atatcaaatg gttaaatgag aaaaacttgg aggtgctcac atgtcatcaa tcgtagtagt
    32101 tgggacacaa tggggagacg aaggaaaagg aaaaataacg gatttcttgg cagaacagtc
    32161 agatgttatc gcgcgttttt caggtggtaa taatgcaggc cataccattc aatttggcgg
    32221 agaaacatat aaattacatt tagtaccatc tggtatcttt tacaaagaca aattagcggt
    32281 aatcggtaac ggagtcgttg ttgatccagt tgcactattg aaagaattag acggattaaa
    32341 tgaacgtggc attcctacaa gtaatttacg tatatctaat cgtgcgcaag tgattttacc
    32401 atatcactta gcacaagatg aatatgaaga acgtttacgt ggtgacaata agattggtac
    32461 aactaaaaaa ggtatcggtc cagcatatgt agacaaagtt caacgtatcg gtattcgtat
    32521 ggcagattta cttgaaaaag aaacattcga aagattatta aaatcaaaca ttgaatataa
    32581 acaagcatat ttcaaaggta tgtttaacga aacatgtcca tcatttgatg atatctttga
    32641 agaatattat gcagcaggtc aacgtctaaa agaatttgta acagacacat caaaaatctt
    32701 agacgatgca tttgtagcag atgaaaaggt acttttcgaa ggtgcgcaag gtgtaatgtt
    32761 agatatcgac catggtacat atccattcgt tacatcaagt aatccaattg caggtaacgt
    32821 tactgttggt acaggtgtag gtcctacatt cgtttcaaag gtaattggtg tatgtaaagc
    32881 ttatacatca cgtgttggtg atggtccatt ccctactgaa ttattcgatg aagatggaca
    32941 tcatattaga gaggttggtc gtgaatacgg tacaacaaca ggacgtccac gtcgtgtagg
    33001 ttggtttgat tcagttgtat tacgtcactc tcgtcgtgta agtggtatta cagatttatc
    33061 tattaactca atcgatgttt taacaggcct agacacagtg aaaatctgta cagcttatga
    33121 attagacggt aaagaaatta ctgagtaccc agcaaactta gatcaattaa aacgttgtaa
    33181 accaatcttt gaagagttac caggttggac agaagacgta acaaatgtgc gtactttaga
    33241 agaattacct gaaaatgcac gtaaatattt agagcgtatt tcagaattat gtaatgtaca
    33301 aatttctatc ttctcagttg gtccagatag agaacaaaca aacctattaa aagaattgtg
    33361 gtagaacttt atataagtca tacacaatga ttataaatac atgagccttc tatctttatt
    33421 ggtaggaggc ttttgttatg cttgcttctg tatcgattcg attatttaga taaaaaatac
    33481 taacgtaaag gcgatatttg ctagtcataa tttagaagat tagatgatat ttaacgaaaa
    33541 ttaagatgaa atacttgaat gtaagaagtc tgatgtcgaa aatagctatt aaaatagagt
    33601 agacgtaagt gtaaatgaaa gcacctaaaa tagaaaaatt tcaaaaatag cgtaattatt
    33661 ataatgaata gactgccaat aaaatgcaat ttttcactta taacattctt caaaaaataa
    33721 tagcaaaatt atgtaaaaaa tatcttgtca tggcaagatt ggctgtgcta taatctatct
    33781 tgtgcttaag aacggctcct tggtcaagcg gttaagacac c
//
LOCUS       DALAGF010000017        33725 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.017, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000017 DALAGF010000000
VERSION     DALAGF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33725
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.017"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            55..315
                     /locus_tag="O2692_000761"
     CDS             55..315
                     /locus_tag="O2692_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCZ4298400.1"
                     /translation="MTKNYKDMTQDELRGLLAEKNAELFDLASEIDEETEFDVLLFSN
                     VGISNGDFTPSSHCVIGNVVDIANLLKRRAVYRDIADVIKMR"
     gene            355..615
                     /locus_tag="O2692_000762"
     CDS             355..615
                     /locus_tag="O2692_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCZ4298401.1"
                     /translation="MYYKTGDVCRKIINVDGFDFQLRVKKRAYSVEMVVLDHEGNSID
                     GLLVSDENDLYTALDILKQSVYEWIENNTDEQDKLMNLVMKW"
     gene            624..887
                     /locus_tag="O2692_000763"
     CDS             624..887
                     /locus_tag="O2692_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298402.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            896..2839
                     /locus_tag="O2692_000764"
     CDS             896..2839
                     /locus_tag="O2692_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCZ4298403.1"
                     /translation="MKINKLTISNFAGIKEEKFNFDGKDAKIYGNNATGKTTTATALQ
                     WLLFDKGLDGSTKSFNPVPLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPKYTIN
                     QKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLDWKKR
                     RSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIKDIPI
                     RINQTQQNKQDVPEFDNDRYAIIKQEIEQLENERIDIQNGKEEINLRNQLADKQSELK
                     RIEDNNSASNENKIHALTNELHVENGTVANLKTRLKQNKQQITHEENRRNQLLENHKG
                     LKSDLEKAKNQKFEYLDDNVCSCCGQKLPVEQVDMAREKALQKFNASKSKELETIQTS
                     INHIISEGKKIKLIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVTQTDE
                     YKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKSEIEVSRSIEKSNKHLDD
                     VISELRNEEDRLLDEKEKYSHDLYILKEFTTTKVKMLTENINNEFEIAEFKLFNTLVN
                     GELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESVTELI
                     KTESQQIQLIVNEQDKKLRMETI"
     gene            2841..3761
                     /locus_tag="O2692_000765"
     CDS             2841..3761
                     /locus_tag="O2692_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCZ4298404.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYT
                     ELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            3842..4459
                     /locus_tag="O2692_000766"
     CDS             3842..4459
                     /locus_tag="O2692_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCZ4298405.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKVL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSTLANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKSEIQKVTGAPVYFGGL"
     gene            4460..4930
                     /gene="ssb"
                     /locus_tag="O2692_000767"
     CDS             4460..4930
                     /gene="ssb"
                     /locus_tag="O2692_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCZ4298406.1"
                     /translation="MLNRTILVGRLTRDPELRTTQNGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVIADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIELNDDDLPF"
     gene            4960..5853
                     /locus_tag="O2692_000768"
     CDS             4960..5853
                     /locus_tag="O2692_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCZ4298407.1"
                     /translation="MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSKAKVP
                     IGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIV
                     NYDFYQSEQGRNQHQNDIKPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKK
                     KTTAFDFFQDNGFGFITSYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAK
                     SILNTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPE
                     VDENLEKDREAFIKRLNSKWE"
     gene            5860..6078
                     /locus_tag="O2692_000769"
     CDS             5860..6078
                     /locus_tag="O2692_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298408.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGLKHTKFNGRRW
                     YLDDYELKTLIDNEQMELGHQTSLFEYI"
     gene            6087..6491
                     /locus_tag="O2692_000770"
     CDS             6087..6491
                     /locus_tag="O2692_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCZ4298409.1"
                     /translation="MEIEIKFNEVFNAPMGSPRPRFSTKGRYAHTYMPTKYTEHKKYL
                     QNQMPKLNLENALKIELEFYFTPPKSWSKKKKTQAIGQLKVTKPDIDNLMKTVLDACN
                     NYLWKDDNQITEITSSKRYGIEPKIIIRIEEI"
     gene            6504..6872
                     /locus_tag="O2692_000771"
     CDS             6504..6872
                     /locus_tag="O2692_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298410.1"
                     /translation="MARKARIVTINDKPYRFTKSEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKKEAELRRKKPHLFNVPQKH
                     PRGRYACYLMENDIFVKVKK"
     gene            6876..7118
                     /locus_tag="O2692_000772"
     CDS             6876..7118
                     /locus_tag="O2692_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HCZ4298411.1"
                     /translation="MTDNARKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGHIV
                     PGWQGVKKTFDTAEELETYIKQQGLEYEEQKQLTLF"
     gene            7127..7498
                     /locus_tag="O2692_000773"
     CDS             7127..7498
                     /locus_tag="O2692_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCZ4298412.1"
                     /translation="MKVNYETGFQIGVMEARLKKMRKQRDEYKKQRDELIGDIAKLRE
                     RNEELENMWRTLKNELFGRYEFYRFRLSELQIESRANKEVAIYRRAEINLSVILCRMD
                     KLDGTNEFYEFLGQMEDDTNE"
     gene            7491..7727
                     /locus_tag="O2692_000774"
     CDS             7491..7727
                     /locus_tag="O2692_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCZ4298413.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEIRE
                     AINAQWVEYPEDWASEVLREVRELEYEEEQENEN"
     gene            7717..7959
                     /locus_tag="O2692_000775"
     CDS             7717..7959
                     /locus_tag="O2692_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298414.1"
                     /translation="MKINESLKKLKEKGYKENEDKAIFNLADGTLEIYIDHDEKTIIT
                     EFHDLKVFVSEDLKDKSMESVMYELAGIDEEDKEND"
     gene            7952..8485
                     /locus_tag="O2692_000776"
     CDS             7952..8485
                     /locus_tag="O2692_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HCZ4298415.1"
                     /translation="MTNILQVKLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDNETLESE
                     DMSNFGRSPAGIDGKYARLPVTDKILCMNGSYVINKGDKLAQLVIVPIWTPELKQVEE
                     FESVSERGAKGFGSSGV"
     gene            8522..8767
                     /locus_tag="O2692_000777"
     CDS             8522..8767
                     /locus_tag="O2692_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298416.1"
                     /translation="MSDMLEIFLIGFGVYLFYRIAIIFLKSKKTIHTNIYEMLMLATI
                     FMISTFAYKHQKTHILIAFLVMFFMSKLKQVQGSYEE"
     gene            8764..8970
                     /locus_tag="O2692_000778"
     CDS             8764..8970
                     /locus_tag="O2692_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCZ4298417.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECGK"
     gene            8967..9353
                     /locus_tag="O2692_000779"
     CDS             8967..9353
                     /locus_tag="O2692_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298418.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGITALLLVVALPIYTVASYQNK
                     EVHQGTITDKYNKRQDKEDKFYIVLDDKQVIENSDLFFKGKFDSADIQARLKVGDKVK
                     VKTIGYRIHFLNLYPVLYEVKKVDKK"
     gene            9350..9499
                     /locus_tag="O2692_000780"
     CDS             9350..9499
                     /locus_tag="O2692_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ4298419.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            9499..9699
                     /locus_tag="O2692_000781"
     CDS             9499..9699
                     /locus_tag="O2692_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCZ4298420.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLKEYRIELKRMNNDIKK"
     gene            9727..10143
                     /locus_tag="O2692_000782"
     CDS             9727..10143
                     /locus_tag="O2692_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ4298421.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            10375..10674
                     /locus_tag="O2692_000783"
     CDS             10375..10674
                     /locus_tag="O2692_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCZ4298422.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KSKRKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            10804..11148
                     /locus_tag="O2692_000784"
     CDS             10804..11148
                     /locus_tag="O2692_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298423.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            11145..12806
                     /locus_tag="O2692_000785"
     CDS             11145..12806
                     /locus_tag="O2692_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCZ4298424.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYE
                     VSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNK
                     EYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF"
     gene            12822..14009
                     /locus_tag="O2692_000786"
     CDS             12822..14009
                     /locus_tag="O2692_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCZ4298425.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            13993..14730
                     /locus_tag="O2692_000787"
     CDS             13993..14730
                     /locus_tag="O2692_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCZ4298426.1"
                     /translation="MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVASNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            14754..15899
                     /locus_tag="O2692_000788"
     CDS             14754..15899
                     /locus_tag="O2692_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCZ4298427.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            15919..16203
                     /locus_tag="O2692_000789"
     CDS             15919..16203
                     /locus_tag="O2692_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298428.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKASSSMVKSEIVDLLNGEDNDD"
     gene            16193..16477
                     /locus_tag="O2692_000790"
     CDS             16193..16477
                     /locus_tag="O2692_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCZ4298429.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            16461..16823
                     /locus_tag="O2692_000791"
     CDS             16461..16823
                     /locus_tag="O2692_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HCZ4298430.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            16820..17224
                     /locus_tag="O2692_000792"
     CDS             16820..17224
                     /locus_tag="O2692_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCZ4298431.1"
                     /translation="MSVKVIGDKALERELENRFGTQAMLKVQDRALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVHWRGSKDRYKIVHLIENGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            17221..17625
                     /locus_tag="O2692_000793"
     CDS             17221..17625
                     /locus_tag="O2692_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298432.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDERAYSYIVQIDVFVKFNEKYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSTRVYEGIFYEEE"
     gene            17629..18273
                     /locus_tag="O2692_000794"
     CDS             17629..18273
                     /locus_tag="O2692_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCZ4298433.1"
                     /translation="MVVKQASAPKAYFNITGLGFAKLTKEGAKLEYSDITKTRGLQKI
                     GVETGGDLKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEEYDEYGVYKET
                     QGKQNNYVAIWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLIDNKKSVRKYIFDSANMTNHSGNGEKGEEAFLKKILGEEYTGNVTEDNGETL"
     gene            18300..18539
                     /locus_tag="O2692_000795"
     CDS             18300..18539
                     /locus_tag="O2692_000795"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCZ4298434.1"
                     /translation="MKSVNIPDSFKTLKVGNTYDLNVVVEPSNQSKLLKYTTDQTNIV
                     SINSDGQVTAEAQGIATVKAYVGNMSDTITINVEA"
     gene            18589..18939
                     /locus_tag="O2692_000796"
     CDS             18589..18939
                     /locus_tag="O2692_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298435.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFAVKDLKERMHAPDGMNALREQVVFITQG
                     QQTEETRNFIQNMK"
     gene            18990..19127
                     /locus_tag="O2692_000797"
     CDS             18990..19127
                     /locus_tag="O2692_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298436.1"
                     /translation="MMDLIENGKDANEVLKMPFHYVLSIYQNKNNDISEEKAEALIDA
                     F"
     gene            19184..23713
                     /locus_tag="O2692_000798"
     CDS             19184..23713
                     /locus_tag="O2692_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCZ4298437.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTDSYKQRIKELDGTITGYKKNVDDLAKQYDKVSQEQGENSAGAQKLRQEYNK
                     QANELNYLERELQKTSAEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGMMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGSELKKLQNSFKDVY
                     GNFPADAETVGGVLGEVNTRLGFTGKELENATESFLKFSHITGSDGVQAVQLITRAMG
                     DAGIEASEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKNPREEFKKTLAEIEKTPDIASATSLAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWASIESAFAPVMEELIKKLSVAVDWFSNLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTIGNAVTVLAPLLAGIAKADGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNDAIESVKQTFSNFIQFIQPFIDSVKNVFKQAVSAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKVIFEFILNFVIKPIMFAIWQVMQFIWPAVK
                     ALIVSTWENIKGVIQGTLNIILGLIKFFSSLFTGDWRGVWDAIIMILKGVVQLIWNLI
                     QLWFVGKILGVVRYFGGLLKGLITGIWDVIKSIFSKSLSAIWNATKSIFGFLFNSVKS
                     IFTNMKNWLSNTWSSIRTNTIGKAQSLFSGVKSKFTSLWNATKDIFSNLRNWMANIWN
                     SIKDNTVGIASRLWSKVRGIFTNMRDGLQSIISKIKSHIGGMVDAIKKGLNKLIEGLN
                     WVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEII
                     EFPNGKRVITPNTDTTAYLPKGSKVYNGTQTYSMLNGTLPRFSLGTMWKDIKSGASSA
                     FNWTKDQIGKGTKWLGDKVGDVLDFMENPGKLLNYILEAFGIDFNSLTKGMGIAGDIT
                     KAAWSKIKKSATDWIKENLEAMGGGDLVGGILDPDKINYHYGRTAAYTAATGRPFHEG
                     VDFPFVYQEVRTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHLKNFSKSPPSGTM
                     VKPGDVVGLTGNTGFSTGPHLHFEMRRNGRHFDPEPYLRNAKKKGRLSIGGGGATSGS
                     GATYASRVIRQAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNNWDINAQRGDPSR
                     GLFQIIGSTFRANAKRGYTNFNNPVHQGISAMQYIVRRYGWDGFKRAGDYAYATGGKV
                     FDGWYNLGEDGHPEWIIPTDPARRNDAMKMLHYAAAEVRGRKASKNKRPSQLSSVNGF
                     DDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQ
                     ESTKVKFRKGGIAIQ"
     gene            23710..25194
                     /locus_tag="O2692_000799"
     CDS             23710..25194
                     /locus_tag="O2692_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCZ4298438.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGFKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFAIPVKFTIKVVLTDPYKYSVTGYKNTAISDQVSVVNSGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFSDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNK
                     PIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRP
                     ASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVI
                     NEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            25210..28995
                     /locus_tag="O2692_000800"
     CDS             25210..28995
                     /locus_tag="O2692_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298439.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPMRYIWGIYEPQSDDQNMNETRLSSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNLIHQKLNDNISNINTIVKDVVDGELEYFERKIHKSDT
                     PPENPVNDTLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTKTLFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDNLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRRTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGNNNRYVQIQNDSIELGGIVQRTWKGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNAG"
     gene            28985..29137
                     /locus_tag="O2692_000801"
     CDS             28985..29137
                     /locus_tag="O2692_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298440.1"
                     /translation="MQDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            29184..29471
                     /locus_tag="O2692_000802"
     CDS             29184..29471
                     /locus_tag="O2692_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298441.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVERSDLSPEVTVNTETV"
     gene            29529..29825
                     /locus_tag="O2692_000803"
     CDS             29529..29825
                     /locus_tag="O2692_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCZ4298442.1"
                     /translation="MGWFKKHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIKL
                     DKTLDAIQKEREIDEKNKKENDKNIRDMKMWVLGLVGTIFGSLIIALLRMLMGI"
     gene            29975..30151
                     /locus_tag="O2692_000804"
     CDS             29975..30151
                     /locus_tag="O2692_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCZ4298443.1"
                     /translation="MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(30204..30311)
                     /locus_tag="O2692_000805"
     CDS             complement(30204..30311)
                     /locus_tag="O2692_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298444.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPNEPVKKTVAILD"
     gene            30363..30617
                     /locus_tag="O2692_000806"
     CDS             30363..30617
                     /locus_tag="O2692_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCZ4298445.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            30629..31384
                     /locus_tag="O2692_000807"
     CDS             30629..31384
                     /locus_tag="O2692_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ4298446.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
     gene            31575..32066
                     /gene="sak"
                     /locus_tag="O2692_000808"
     CDS             31575..32066
                     /gene="sak"
                     /locus_tag="O2692_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HCZ4298447.1"
                     /translation="MLKRGLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVII
                     EKK"
     gene            32716..33051
                     /locus_tag="O2692_000809"
                     /pseudo
     CDS             32716..33051
                     /locus_tag="O2692_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(33146..33595)
                     /locus_tag="O2692_000810"
     CDS             complement(33146..33595)
                     /locus_tag="O2692_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-inhibiting protein CHIPS"
                     /protein_id="HCZ4298448.1"
                     /translation="MKKKLATTVLALSFLTAGISTHHHSAKAFTFEPFPTNEEIESNK
                     KMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTV
                     YLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY"
BASE COUNT        12811 a         4689 c         6748 g         9477 t
ORIGIN      
        1 attaacaaaa ttaattcatt ttcaatataa aacgaaacaa ggaggaagtc aaccatgact
       61 aaaaattata aagatatgac tcaagacgaa ttaaggggtt tattggctga aaagaatgca
      121 gaattgtttg atttagcgag cgaaatcgat gaagaaactg aatttgacgt tttacttttt
      181 tcaaatgtag ggattagcaa cggagatttt acaccgtcgt cacattgtgt gattgggaat
      241 gttgttgata ttgctaattt attgaaacgt agagctgttt accgagatat cgctgatgtt
      301 atcaaaatgc gttaaaaatt ctagctatag ataatgacaa ggaggacgag aacaatgtat
      361 tacaaaacgg gtgacgtatg tcgaaaaata attaatgtag atggctttga ttttcaatta
      421 agagttaaga aacgagcgta tagcgtcgaa atggttgttc tagaccatga aggaaattca
      481 attgacgggt tactagtttc tgatgagaac gatctataca cagctttaga tattttgaaa
      541 caaagtgttt atgaatggat tgaaaataac acagatgaac aggacaaact aatgaactta
      601 gtcatgaaat ggtaggtata agcatgagag atacagaaag aaatatattg aatattttta
      661 agacgttatt cgacgaatat actttgtcaa accaacgagc attattggaa attgaacgta
      721 atcatcacgg atacttatcg attaatttct tgcactatca cgacagttac aaaacaaaca
      781 ataagcttgt gcagatacat gaaatcaatc cagacagcca tgaacgaata aaaaatttaa
      841 ttatcgaggt actaagaggt catcggaaga ttaaaaaagg agcatgagga aggatatgaa
      901 aataaataaa ttaacaatat cgaactttgc cggaatcaaa gaagaaaaat ttaattttga
      961 cggtaaagat gcaaaaatat acggcaataa tgcgactggt aagactacaa cagcaaccgc
     1021 attacaatgg ttgcttttcg ataaaggttt ggacggttca accaaatcat ttaaccctgt
     1081 acctttaaac gaaaaaaacg aagaaaatta tgagttaatt ccgactgttt tcgcagaatt
     1141 tgaaatcgac ggaaaaatta cgacttttaa aaaagagtca catcctaaat acacaataaa
     1201 tcaaaagacg aatcgcaagg aatactcacg aagtcgaacg aagaaacaat atatcaatga
     1261 tgaatcaata aaagtaaagg attataaagc tcgtattgat gaacttattg atgaagatgt
     1321 attcaagtta attacgaacc ctcaagcatt taacttacta gattggaaga agcgaagaag
     1381 tttgttgttt gaaattgcta aaccaatcaa tgatgaggat gtcattaaaa caaatgatga
     1441 ttttaaagaa ctaaataata ttcttggaga tcatgaaatt gaaacaaaga aaaagattct
     1501 tacggacaag ataaaacaga ttaacaaaga tatcaaagat attccgatac gtattaacca
     1561 aacacaacaa aataagcagg atgtaccaga attcgataat gatagatacg caattatcaa
     1621 acaagaaatt gagcaacttg aaaatgagcg tatagatatt caaaacggta aggaagaaat
     1681 taatttacgt aatcaattag ctgataaaca atcagaattg aaacgcatag aagacaataa
     1741 cagcgcaagt aatgagaaca aaatccatgc tttaacaaat gaattacacg ttgaaaatgg
     1801 aacggtagca aaccttaaaa cgagattaaa gcaaaacaaa caacaaatca cgcatgaaga
     1861 aaatagacgt aatcaattat tagaaaatca taaaggatta aaaagtgatt tagaaaaagc
     1921 taaaaatcaa aaatttgaat atcttgatga caatgtatgt agttgttgtg gtcaaaagtt
     1981 accagttgaa caagtggata tggcaagaga aaaagcattg cagaaattca atgctagcaa
     2041 atcgaaagaa ttagaaacaa tacaaacatc tatcaatcac attatttcag aaggcaagaa
     2101 aataaagcta atcatcgaga agttagagga tgacaataat aatcttcaaa ttaaaatcaa
     2161 cgaagcagaa gagcgttcag caagaataca aaacaaaatt aataagttga aaacaactca
     2221 cgttgacgtt acgcaaactg acgaatacaa agcagtaatg ttagagataa atgagattaa
     2281 tcaaaaacgc tctaacatca ggaaaactat tcaagataaa gtttcaggaa tagatgacaa
     2341 aataagcgaa cttactcaag aaaaatcaga aattgaagtg tcaagatcaa tcgaaaaatc
     2401 aaataaacat ctagatgatg ttatttctga attaagaaat gaagaagaca gattattgga
     2461 tgaaaaagaa aagtattcac atgaccttta tatcttaaaa gaatttacaa caacaaaagt
     2521 caaaatgctt actgaaaaca tcaataatga atttgaaatt gctgaattta agctattcaa
     2581 taccttagtt aacggcgaat tagaagaaac atgttccaca acggttaacg gcgtcgaata
     2641 cgacagcggt ttaaataacg cctcaagaat taatgttggc ttagatatca tcaatacact
     2701 gtcaaaacat tttaaagtta cagcgccaat atttattgat aatgctgaat cagtaacaga
     2761 gcttatcaaa acagaatcac aacaaattca attgatagta aatgaacaag ataaaaaatt
     2821 aagaatggag actatataaa atgactgaaa ataataaatt acaaactatt gaacaacaat
     2881 tagtacaaga aaagaacgta tctgacaacg tattaaacaa agtgagagtt ttagagtcac
     2941 aaggcaattt ggaattgcca aatgattatt caccaagtaa tgccatgaaa caagcatggt
     3001 tacaaatcag ccaagataac aaattaatga gttgtaacga tacaagcaaa gcaaatgcct
     3061 tattagacat ggtaacgcaa ggtttaaatc cagctaaaaa tcaatgctac tttattcctt
     3121 acggcaacaa aatgcagtta caacgtagct atcacggtaa tgtaatgatg ttaaaacgtg
     3181 atgcaggtgc tcaagatgtt gttgctcaag tgatttataa aggcgataca ttcaagcaag
     3241 aaatgggaga aacaggacgt atcaaagcga ttaaacacga acaagacttc tttaacatcg
     3301 acaaagaaaa cattatcggt gcgtactgca caatcgtatt taatgatgga cgagataact
     3361 atattgaagt catgactatt gaacaaatta aacaagcatg gatgcagtca tcaatgatta
     3421 aagatgaaaa agcattacaa aattctaaaa cacataataa tttcaaagaa gaaatggcta
     3481 aaaaaacagt tatcaataga gctgctaaac gttatatcaa cacatcaaca gatagcaatc
     3541 ttttcaaata cgcacaagaa tccgaacaac gtcaacgcaa agaagtgttg gacgcagaag
     3601 ttgaagaaaa tgcaaatcaa gaacaattgg actttgaaca accagttctt gaagaagcac
     3661 aatacacaga attagaaaat gataagccta ttgatgtatc tgactttgaa gaaataaaag
     3721 aacctgcaac agaaaaagaa agcgaagaag agccatttta attgaaacaa tagcaactgg
     3781 ttcaagtggt aactgctacg tcttaaatga tggacgtact acgttactac ttgaggcagg
     3841 tataaaattt gaacgtgttc aaaagcattt caaatataaa acaagacata tagcagggtg
     3901 tcttatcaca cacgaacatg gtgatcatgc aaagtataca aagcagtttg tcgacaatgg
     3961 tgtaatcagc tatatgactg ctggaacaca acaagctatg aattttgaaa gtcatcgctt
     4021 atgcacgatt aaggcaaagc aagagctgcg aataggtaca tggtcaattc taccgtttga
     4081 catcgaacat gatgctaacg agcctgtggc tttcttatta caaagcacac taggttataa
     4141 ggttctgtat gttactgata caaagtattt gaaatacaaa tttaatggca ttacgcacat
     4201 gatgctagaa gttaattata tctatgaaca aatgcaagaa aacataaaaa acggcagtgt
     4261 gcacagcaca ttagcaaaca gaattatgga gtctcatttt agcttagaac atgctatcgg
     4321 aatgttaaaa gcaaatgatt taactagact cgaagaaata catttaattc atttaagtag
     4381 tcaaaattca aatgcaaaat acattaaaag tgaaatacaa aaagtgacgg gcgcgcccgt
     4441 ttattttgga ggtttataaa tgctaaacag aacaatatta gttggtcgtt taactagaga
     4501 cccagaatta agaaccactc aaaatggtgt aaatgtagca tcattcacat tagcagttaa
     4561 ccgtacattt acaaatgcac aaggcgagcg cgaggcagac tttattaata tcatcgtatt
     4621 taaaaaacaa gcagagaacg ttaataaata cctatctaaa ggatcgttgg cgggcgtaga
     4681 tggtaggtta caaacgcgga actatgaaaa taaggaaggt caacgtgtat acgttacgga
     4741 agttattgct gatagtattc aatttttaga accgaaaaac tcaaatgaca ctcaacaaga
     4801 tttatatcaa caacaagtac aacaaacacg tggacaatcg caatattcaa ataacaaacc
     4861 agtaaaagat aatccgtttg cgaatgcaaa tggtccgatt gaactaaatg atgatgattt
     4921 accattctaa tttaaccggt ttgaaagtga ggtgtgtata tgactggttg gataagtatt
     4981 gatcgctcaa ttcaaaatca ttggctattt aaagaaaaga gaacattttc aaagtttgaa
     5041 gcatggatat atttactcat ggaagcgaat cattcaaagg caaaagtgcc tataggaaac
     5101 caaattgtaa ccgttgaaag aggacaaaga ttaacatcta ttttgacctt gtctgacctt
     5161 tttaactggt cacgatttaa agtgaaaacc ttccttgact tactcgagag tgatggaatg
     5221 ttagaagtca aaacaacatc aaaatatacc cttataacca ttgtcaatta tgacttttat
     5281 caaagtgagc agggcaggaa ccaacatcaa aacgacatca aaccaacatc aaaacaacat
     5341 cagtcaaaca tcaacccaac atcaaaacaa catcaaacca acacaaacaa taatgataat
     5401 aaagataata atgaaaagaa tgtgaataat gagaagaaga agacaaccgc cttcgacttc
     5461 ttccaagata acggattcgg tttcataact tcttacaatt tagacgattt aaattattat
     5521 cttgattcat ttgaaaatga ttcagatgaa atagttaccg catcacttaa aatcgctaaa
     5581 gacagaaaca aagttacttg gggatatgct aaaagcattt tgaatacatg gcttaatgca
     5641 aacttgaaat ctattgaaca agtacgtgca tttgaaaagc aacaacttga aagcaaaaaa
     5701 caaaattata aacctttcgt taaacaatca aaagaaaaaa cacccaaatg gctcacagac
     5761 agcacgagag aaacgaaaac gccggaagta gatgaaaacc ttgagaaaga cagagaagct
     5821 tttattaagc gtctaaatag caaatgggag tgattgaaaa tggatgcatt tgataaatac
     5881 tatctatttg atcatgacgg caacaaaatg ttttcagtta caccacattt taaagatgga
     5941 cggcatttag ttgttggatt aaaacacaca aaattcaatg gtcgacgttg gtacttagat
     6001 gattatgaat taaaaacact tattgataat gaacaaatgg agttaggaca ccaaacaagc
     6061 ttatttgaat atatatgagg gattacatgg agatagaaat taaatttaat gaagtgttta
     6121 atgcgccgat gggctcgcct cgaccgcgtt ttagcacaaa aggtagatat gcacacacat
     6181 atatgcctac aaaatataca gaacataaaa aatatttaca aaatcaaatg ccaaagctaa
     6241 atctagaaaa tgcattaaaa attgaattag agttttactt tactccacct aaatcatggt
     6301 cgaagaaaaa gaaaactcaa gcgattggac aattaaaagt aactaaacca gatatcgata
     6361 atttgatgaa gacagttcta gacgcttgta acaattattt gtggaaagac gataaccaaa
     6421 ttacagaaat aactagctca aagcgttatg gaattgagcc caaaataatc atacgaatag
     6481 aagaaatata agaggtggat aaaatggcga gaaaagcaag gattgtaaca ataaatgata
     6541 aaccatatag gtttaccaaa tctgaaatgg aattaataga aagtcacggt ataaccgctg
     6601 gaatggtttc taagagagta aaagacggtt gggaactaca tgaagcaatg gacgcaccag
     6661 aaggtacgcg tttaagcgag tacagagaaa agaaaacaat agaaagactg gaacaagcta
     6721 gactcgaacg caaattggaa agaaagcgaa agaaagaggc tgagctaaga agaaagaagc
     6781 cacatttgtt taatgtacca cagaaacatc caagaggacg ttatgcgtgc tacctgatgg
     6841 aaaacgacat attcgtgaaa gttaagaagt agatcatgac agataacgca cgcaaagaat
     6901 acctaaatca attctttgga tctaagagat atctgtatca ggataacgaa cgagtggcac
     6961 atattcatgt agtaaacggc acttattact ttcatgggca tatcgtacca ggttggcaag
     7021 gcgtgaaaaa gacatttgat acagctgaag agcttgaaac atatataaag caacagggtt
     7081 tggaatatga ggaacagaag caactaactt tattttagag gaggttatga aagtgaacta
     7141 tgaaacagga ttccaaatag gcgtaatgga agctaggttg aagaagatga gaaaacaacg
     7201 tgatgagtac aagaagcaac gtgacgagct tattggggat atagctaagt taagagagcg
     7261 taacgaagag ctggagaaca tgtggcgcac gcttaaaaat gaattgtttg gaagatacga
     7321 attttaccgt tttagactta gcgaactaca gattgagagc agagcgaaca aggaagtagc
     7381 tatatataga agagctgaaa tcaacttaag tgttatattg tgccgaatgg acaaactaga
     7441 cggaacaaat gagttctacg aatttttagg gcaaatggag gatgacacaa atgaataacc
     7501 gcgaacaaat cgaacaatca gttatcagtg ctagcgcgta taacggcaat gacacagagg
     7561 gattgctaaa agagattgaa gacgtgtata agaaagcgca agcgtttgat gaaattcgcg
     7621 aagctattaa tgcgcaatgg gttgagtatc cagaagactg ggcgtcagag gttttgagag
     7681 aagtaagaga gcttgaatat gaggaggaac aggaaaatga aaattaatga atctttgaag
     7741 aaattaaaag aaaaaggata caaagaaaat gaagataaag ctatttttaa tttagctgat
     7801 ggtacgctag aaatttacat cgaccatgac gaaaaaacaa taattacaga attccatgat
     7861 ttaaaggtat tcgtgtcaga agatttaaaa gataaaagta tggagagcgt tatgtatgaa
     7921 ttggctggaa ttgatgagga ggacaaggaa aatgactaac atcctacaag taaagctatt
     7981 atcagaaaac gctagaatgc cagaacgaaa tcataagacg gatgcaggtt atgacatatt
     8041 ctcagctgaa actgtcgtac ttgagccaca agaaaaggca gtgatcaaaa cagatgtagc
     8101 tgtaagcata ccagagggct atgtcggact attaactagc cgtagtggtg taagtagtaa
     8161 aacgcattta gtgattgaaa caggcaagat agacgcggga tatcacggca atttagggat
     8221 taatatcaag aatgataatg aaacgttaga gagtgaggat atgagtaact ttggtcggag
     8281 tcctgctggg atagatggaa agtatgcccg actacctgta acagataaaa ttttatgtat
     8341 gaatggtagt tatgtcataa acaaaggcga caaactagct caattggtta ttgtgcctat
     8401 atggacacct gaactaaagc aagtggagga attcgagagt gtttcagaac gtggagcaaa
     8461 aggcttcgga agtagcggag tgtaaagaca tattagatcg agtcaaggag gttttgggga
     8521 agtgagcgac atgttagaaa tatttttaat agggtttggc gtttatctct tttatcgcat
     8581 agcaattatt tttcttaaga gtaaaaagac tatacacaca aacatatatg aaatgttaat
     8641 gcttgctact atctttatga tatctacatt tgcttataaa catcaaaaga cgcatatctt
     8701 aatagcattt ttagtaatgt tttttatgag taagctcaaa caagttcaag ggagctatga
     8761 ggaatgacac aatacttagt cacaacattc aaagattcaa caggacgtaa acatacacac
     8821 ataactaaag ctaagagtaa tcaaaggttt acagttgttg aggcagagag taaagaagaa
     8881 gcgaaagaga agtacgagaa acaagttaaa agggatgcag ttattaaagt gggtcagttg
     8941 tttgaaaata taagggagtg tgggaaatga ttaaaaaact taaaaatatg gatgggttcg
     9001 acatctttat tgttggaata ctgtcattat tcggtataac cgcattgcta cttgttgtcg
     9061 cattgcctat ctatacagtg gctagttacc aaaacaaaga agtacatcaa gggacaatta
     9121 cagataaata taacaaaaga caagataaag aggacaaatt ctatattgta ttagatgata
     9181 aacaagtcat cgaaaactca gacttattct tcaaaggaaa gtttgatagc gcagacatac
     9241 aagctaggtt aaaagtaggt gataaagtaa aagttaagac gattggatat agaatacact
     9301 ttttaaattt atatccggtc ttatacgaag taaagaaggt agataaaaaa tgattaagca
     9361 aatactaaga ttattattct tactagcgat gtatgagtta ggtaagtatg taactgagca
     9421 agtatatatt atgatgacgg ctaatgatga tgtagaggcg ccgagtgatt acgtctttcg
     9481 agcggaggta agtgagtgat gtggattact atgactattg tatttgctat attgctatta
     9541 gtttgtatca gtattaatag tgatcgtgca agggagatac aagcgctcag atatatgaat
     9601 gattatctac ttgatgaagt agttaaaact aaaggataca acgggttaaa agaatacagg
     9661 attgaattaa agcgaatgaa taacgatatt aaaaagtaat ttatattatc ggaggtatta
     9721 cattgaatga taaagattga gaaacacgat atcaaaaagc ttgaagaata cattcagcac
     9781 atcgataact atcgaagaga gttgaagatg cgagaatatg aattacttga aagtcatgaa
     9841 ccagataatg cgggagctgg caaaagtaat ttgccgggta acccgattga acgatgtgca
     9901 ataaagaagt ttagtgataa caggtacaat acattaagaa atatagttaa cggtgtagat
     9961 agattgatag atgaaagtga tgaggatacg cttgagttat taaggtttag atattgggat
    10021 tgtcctattg gttgttatga atgggaagat atagcacatt actttggtac aagtaagaca
    10081 agtatattac gtagaaggaa tgcactgatc gataagttag caaagtatat tggttatgtg
    10141 tagcggactt ttaccctatg taagtccgca ttaaaacagt ttattatgtt agtatcagat
    10201 taatatttaa agttattaaa tgctaatacg acgcatgaac aagaggcgca tcactatgtg
    10261 atgtgtcttt ttatttatga ggtatgaaca tgttcaaact aatagtaaat acattactac
    10321 acatcaagta tagatgcgtc ttgatactac ttaagttata taaggtgaaa cattatgatg
    10381 actaaagacg aacgcatacg attctataag tctaaagaat ggcaaacaac aagaaaaaga
    10441 gtgctagaaa gagataatta tgaatgtcaa caatgtaaga gagacggcaa gttaacgaca
    10501 tatgacaaaa gcaagcgtaa gtcgttggat gtagatcata tattatcgct agaacatcat
    10561 ccggagtttg ctcatgactt aaacaattta gaaacactgt gtattaaatg tcacaacaaa
    10621 aaagaaaaga gatttataaa aaaagaaaat aaatggaaag acgaaaaatg gtaaataccc
    10681 ccgggtcaaa aaaatcaaaa gcgatcaaaa tacttgggga acggggaggg gctcgacttc
    10741 gcgataattt taaaaatcca tgtatacccc ccctcttata accattttaa ggcaggtgat
    10801 gaaatggaga ttatagttga tgaaaactta gtgcttaaag aaaaagaaag gctgcaagta
    10861 ttatataaag acatacctag caataaatta aaagtagttg atggtttaat tattcaagca
    10921 gcaaggctac gtgtaatgct tgattacatg tgggaagaca taaaagaaaa aggtgactat
    10981 gatttattta ctcaatctga aaaggcgcca ccatatgaaa gggaaagacc agtagccaaa
    11041 ctatttaatg ctagagatgc tgcatatcaa aaaataatca aacaattatc ggatttattg
    11101 cccgaagaga aagaagacac agaaacgcca tctgatgatt acctatgatt agtaataaat
    11161 acgttgatga atatataaat ttgtggaaac aaggaaagat aattttaaat aaagaaagaa
    11221 ttgatctctt taattatcta caaaaacata tatattcacg agatgatgta tattttgatg
    11281 aacagaaaat cgaggattgt atcaaattta ttgaaaaatg gtattttcca acattaccat
    11341 ttcaaaggtt tatcatagct aatatatttc ttatagataa aaatacagat gaagctttct
    11401 ttacagaatt tgctattttc atgggacgtg gaggcgggaa aaacggtcta ataagtgcta
    11461 ttagtgattt tctttctacg cccttacacg gagttaaaga atatcacatc tccattgttg
    11521 ctaatagtga agatcaagca aaaacatcgt ttgatgaaat cagaaccgtt ttaatggata
    11581 acaaacgaaa taagacgggt aaaacgccaa aagctcctta tgaagttagt aaagcaaaaa
    11641 taataaaccg tgcaactaaa tcggttattc gatataacac atcaaacaca aaaaccaaag
    11701 acggtggacg tgaggggtgt gttatttttg atgaaattca ttatttcttt ggtcctgaaa
    11761 tggtaaacgt caaacgtggt ggattaggta aaaagaaaaa tagaagaacg ttttatataa
    11821 gtactgatgg ttttgttaga gagggttata tcgatgcaat gaagcacaaa attgcaagtg
    11881 tattaagtgg caaggttaaa aatagtagat tgtttgcttt ttattgtaag ttagacgatc
    11941 caaaagaagt tgatgacaga cagacgtggg aaaaggcgaa cccaatgtta cataaaccgt
    12001 tatcagaata cgctaaaaca ctgctaagta cgattgaaga agaatataac gatttaccat
    12061 tcaaccgttc aaataagccc gaattcatga ctaagcgaat gaatttgcct gaagttgacc
    12121 ttgaaaaagt aatagcacca tggaaagaaa tactagcgac taatagagag ataccaaatt
    12181 tagataatca aatgtgtatt ggtggtttag actttgcaaa cattcgagat tttgcaagtg
    12241 tagggctatt attccgaaaa aacgatgatt acatttggtt aggacattcg tttgtaagac
    12301 aagggttttt ggatgatgtc aaattagaac ctcctattaa agaatgggaa aaaatgggat
    12361 tattgaccat tgtcgatgat gatgtcattg aaattgaata tatagttgat tggtttttaa
    12421 aggctagaga aaaatatggg cttgaaaaag tcatagctga taattataga actgatattg
    12481 taagacgtgc gtttgaggat gctggcataa aacttgaagt acttagaaat ccaaaagcaa
    12541 tacatggatt acttgcacca cgtatcgata caatgtttgc gaaacataac gtaatatatg
    12601 gagacaatcc tttgatgcgt tggtttacta ataatgttgc agtaaaggtt aaacccgatg
    12661 gtaataaaga atatattaaa aaagatgaaa atagaagaaa aaccgatggg ttcatggctt
    12721 ttgttcacgc attatataga gcagacgata tagtagacaa agacatgtct aaagcgcttg
    12781 atgcattaat gagtatagat ttctaataga ggaggtgaga catgagtatt ctagaaaaga
    12841 tatttaaaac taggaaagat ataacatata tgcttgattt agatatgata gaagatctat
    12901 cacaacaagc gtatgtgaaa cgtttagcga ttgatagttg tattgaattt gttgcgcgag
    12961 ctgtcgctca aagtcatttt aaagtattgg aaggtaatag aattcaaaag aatgatgttt
    13021 actacaagtt aaatataaaa ccaaatactg acttatcaag cgatagtttt tggcaacaag
    13081 ttatatataa actaatttat gataacgagg ttttaatcgt agtaagtgac agcaaagaat
    13141 tacttatcgc agatagcttt tacagagaag agtacgcttt gtatgatgat atattcaaag
    13201 atgtaacggt taaagattat acttatcaac gtactttcac aatgcaagag gtcatatatt
    13261 taaagtacaa caacaataaa gtgacacact ttgtagaaag tctattcgaa gattacggga
    13321 aaatattcgg aagaatgata ggtgcacaat taaaaaacta tcaaataaga gggattttga
    13381 aatctgcctc tagcgcatat gacgaaaaga atatagaaaa attacaagcg ttcacaaata
    13441 aattattcaa tacttttaat aaaaatcaac tagcaatcgc gcctttgata gaaggttttg
    13501 attatgagga attatctaat ggtggtaaga atagtaacat gcctttttct gaattgagtg
    13561 agctaatgag agatgcaata aaaaatgttg cgttgatgat tggtatacct ccaggtttga
    13621 tttacggaga aacagctgat ttggaaaaga acacgcttgt atttgagaag ttctgtttaa
    13681 cacctttatt aaaaaagatt cagaacgaat taaacgcgaa actcataaca caaagcatgt
    13741 atttgaaaga tacaagaata gaaattgtcg gtgtgaataa aaaagaccca cttcaatatg
    13801 ctgaagcaat tgacaaactt gtaagttctg gttcatttac aaggaatgag gtgcggatta
    13861 tgttaggtga agaaccatca gacaatcctg aattagacga atacctgatt actaaaaact
    13921 acgaaaaagc taacagtggt gaaaatgatg aaaaagaaaa agatgaaaac actttgaaag
    13981 gtggtgatga agatgaaagc ggagattaaa ggcgtcatcg tttccaacga agataaatgg
    14041 gtttacgaaa tgcttggtat ggattcgact tgtcctaaag atgttttaac acaactagaa
    14101 tttagtgatg aagatgttga tattataatt aactcaaatg gtggtaacct agtagctggt
    14161 agtgaaatat atacacattt aagagctcat aaaggcaaag tgaatgttcg catcacagca
    14221 atagcagcaa gtgcggcatc gcttatcgca atggctggtg accacatcga aatgagtccg
    14281 gttgctagaa tgatgattca caatccttca agtattgcgc aaggagaagc gaaagatcta
    14341 aatcatgctg cagaaacatt agaacatgtt ggtcaaataa tggctgaggc atatgcggtt
    14401 agagctggta aaaacaaaca agaacttgta gaaatgatgg ctagggaaac gtggctaaat
    14461 gctgatgaag ccattgaaca aggttttgcg gatagtaaaa tgtttgaaaa cgacaatatg
    14521 caaattgtag caagcaatac acaagtgtta tcgaaagatg tattaaatcg tgtaacagct
    14581 ttggtaagta aaacgccaga ggttaacatt gatattgacg caatagcaaa taaagtaatt
    14641 gaaaaaataa atatgaaaga aaaggaatca gaaatcgatg ttgcagatag taaagtatca
    14701 gcaaatggat tttcaagatt ccttttttaa tacaaaaaat aggaggtcat aaaatgacta
    14761 taaatttatc ggaaacattc gcaaatgcga aaaacgaatt tattaatgca gtaaacaacg
    14821 gtgaaccgca agaaagacaa aatgaattgt acggtgacat gattaaccaa ctatttgaag
    14881 aaactaaatt acaagcaaaa gcagaagctg aaagagtttc tagtttacct aaatcagcac
    14941 aatctttgag tgcaaaccaa agaagtttct tcatggatat caataaaaac gttaactata
    15001 aagaagaaaa acttttgcca gaagaaacaa ttgatagaat ttttgaagat ttgacgacga
    15061 atcatccgtt attagctgat ttaggtatta aaaacgctgg tttgcgtttg aagttcttaa
    15121 aatctgaaac ttctggcgta gccgtttggg gtaaaatcta tggtgaaatt aaaggtcaat
    15181 tagatgctgc gttcagtgaa gaaacagcaa ttcaaaataa attgacagcg tttgttgttt
    15241 taccaaaaga tttaaatgat tttggtcctg cgtggattga aagatttgtt cgtgttcaaa
    15301 tcgaagaagc atttgcagtg gcgcttgaaa ctgcgttctt aaaaggtact ggtaaagacc
    15361 aaccaatcgg cttaaaccgt caagtacaaa aaggtgtatc ggtaactgag ggtgcttatc
    15421 cagagaaaga agaacaaggt acgcttacat ttgctaatcc gcgcgctaca gttaatgaat
    15481 tgacgcaagt gtttaaatac cactcaacta acgagaaagg taaatcagta gcggttaaag
    15541 gtaatgtaac aatggttgtt aatccgtccg atgcttttga ggttcaagca cagtatacac
    15601 atttaaatgc aaatggcgta tatgttactg ctttaccatt taatttgaat gttatcgagt
    15661 ctacagtcca agaagcaggt aaggttttaa cgtacgttaa aggtttatat gatggttatt
    15721 tagctggtgg tattaatgtt cagaaattta aagaaacact tgcgttagat gatatggatt
    15781 tatacactgc aaaacaattt gcttacggca aagcgaaaga taataaagtt gctgctgttt
    15841 ggaaattaga tttaaaagga cataagccag ctttagaagg taccgaagaa acactataaa
    15901 attttatgag gtgataaaat ggtgaaattt aaagttgtta gagcttttaa agacatagag
    15961 cacaatcaac acaagtacaa agtaggggag ttgtatccag ctgaagggta taacaatcct
    16021 cgtgttgaat tgttgacaaa tcaaatcaaa aataagtacg acaaagttta tatcgtacct
    16081 ttagataagc tgacaaaaca agaattatta gaactatgcg aatcattaca aaaaaaagcg
    16141 tctagttcaa tggttaaaag tgaaatcgtc gacttattga atggtgaaga caatgacgat
    16201 tgatgatttg cttgtcaaat ttaaatcact tgaaaagatt gaccataatt cagaggatga
    16261 gtacttaaag cagttgttaa aaatgtcgta cgagcgtata aaaaatcagt gcggagtttt
    16321 tgaattagag aatttaatag gtcaagaatt gatacttata cgcgctagat atgcttatca
    16381 agatttatta gaacacttca acgataatta cagacctgaa ataatagatt tttcgttatc
    16441 tctaatggag gtatcagaag atgaagaaag tgtttaaaaa acctagaatt acaactaaac
    16501 gtttaaatac tcgtgttcat ttttataagt atactgaaaa taatggtcca gaagctggag
    16561 aaaaagaaga aaaattatta tatagctgtt gggcgagtat tgatggtgtc tggttacgtg
    16621 aattagaaca agctatctca aacggaaccc aaaatgacat taaattgtat attcgtgatc
    16681 cgcaaggtga ttatttaccc agtgaagaac attatcttga aattgaatca agatatttca
    16741 aaaatcgttt gaatataaag caagtatcac cagatttgga taataaagac tttattatga
    16801 ttcgtggagg atatagttca tgagtgtgaa agtgataggt gataaagcat tagaaagaga
    16861 attagaaaat cgtttcggaa ctcaagcgat gttaaaagtt caagataggg cgctaattgc
    16921 tggtgctaaa gtaattgttg aagaagtaaa aaaacaacta aagccatcaa aagatacggg
    16981 cgcattaatt aatgaggtaa gttttagtaa acctgaatgg ataaacggaa aacgtacaat
    17041 tactgttcat tggcgaggtt ctaaagaccg ttataaaatc gtacatttaa ttgaaaatgg
    17101 acacgttcaa aaagggacag gtaaatttat caaacctaaa gctatgggtg gcgttaatag
    17161 agcaataaga caagggcaaa ataagtattt tgagacgcta aaaagggagt taaaaaaatt
    17221 gtgattgata ttttgtacaa agttcatgaa gtgattagtc aagacagaat tattagagag
    17281 cacgtaaata tcaataatat taagttcaat aaatacccaa acgtaaaaga tacggatgta
    17341 cctttcatag ttattgacga tatagacgac ccaataccta caacttatac tgacggagat
    17401 gagcgcgcat atagttacat tgtacaaata gatgtatttg tgaagtttaa cgaaaagtat
    17461 aacgcgagaa tcataagaaa taagatatct aatcgtattc aaaagttatt atggtctgaa
    17521 ctgaaaatgg gaaatgtatc caacggaaaa cctgaatata tagaagaatt taaaacatat
    17581 agaagcactc gtgtctacga gggcattttt tacgaggagg aataataaat ggtagtaaaa
    17641 caagcaagtg cgccgaaagc gtattttaac attactggtt taggtttcgc taaattaaca
    17701 aaagaaggcg caaagctaga atatagcgat attacaaaaa ctagaggatt acaaaaaatc
    17761 ggtgttgaaa ctggaggaga cttgaaaaca gcatatgctg atggtggtcc aatcgaatca
    17821 gggaacacag acggagaagg taaaatttcg ttacaaatgc atgctttccc taaagagatt
    17881 cgcaaaatag tattcaatga agagtatgat gaatacggtg tttataaaga aactcaaggt
    17941 aaacaaaaca actatgtagc aatttggttc agacaagagc gtcgagacgg cacatttaga
    18001 acggttttat tacctaaagt catgtttaca aatcctaaaa tcgatggcga aacggctgag
    18061 aaagattggg atttctcaag cgaagaggtt gaaggtgagg cacttttccc tttaattgat
    18121 aataaaaagt cagtacgtaa atatatcttt gattcagcaa acatgacaaa tcatagtgga
    18181 aatggtgaaa aaggcgaaga ggctttctta aagaaaattt taggcgaaga atatactgga
    18241 aacgtgacag aggataacgg cgaaactttg taacgcaacc ggcttcatcg gaaactgaga
    18301 taaagtcggt taatatacca gatagtttta aaacactaaa agttggcaac acatatgatt
    18361 taaatgttgt agtagagcca tctaatcaaa gtaagttatt gaaatacaca acagatcaaa
    18421 cgaatattgt atcaatcaat agtgatggtc aagtcactgc ggaagcacaa ggtattgcca
    18481 cggttaaagc atatgttggt aatatgagtg acactataac aataaatgta gaagcataag
    18541 aggggacaac ccctctattt tatttgaaaa taaggagagt attataaaat ggcaaaatta
    18601 aaacgtaaca ttattcaatt agtagaagat ccaaaagcaa atgaaattaa attacaaacg
    18661 tacttaacac cacacttcat ttcatttgaa attgtatacg aagcaatgga tttaatcgat
    18721 gatattgagg acgaaaatag cacgatgaag ccaagagaaa tcgctgacag attgatggat
    18781 atggttgtaa aaatttacga taaccaattc gcagttaaag acctaaaaga acgtatgcat
    18841 gcacctgatg gaatgaatgc acttcgtgaa caagtggttt tcattacgca aggtcaacaa
    18901 actgaggaaa ctagaaattt tatccagaac atgaaataaa gcctgaagat ttaacatata
    18961 aagcaatgtt gaaaaatatg gatactctca tgatggactt aattgaaaat ggtaaagacg
    19021 ctaacgaagt tttaaaaatg ccatttcatt atgtgctttc catatatcaa aataaaaaca
    19081 atgacatttc tgaagaaaaa gcagaggctt taattgatgc attttaacct taaccgtttg
    19141 gttagggtta tttttttgaa cttttttaga aaggaggtaa aaaatgggag aaagaataaa
    19201 aggtttatct ataggtttgg atttagatgc agcaaattta aatagatcat ttgcagaaat
    19261 caaacgaaac tttaaaactt taaattctga cttaaaatta acaggcaaca acttcaaata
    19321 taccgaaaaa tcaactgata gttacaaaca aaggattaaa gaacttgatg gaactatcac
    19381 aggttataag aaaaacgttg atgatttagc taagcaatat gacaaggtat ctcaagaaca
    19441 gggcgaaaac agtgcaggag ctcaaaagtt acgacaagaa tataacaaac aagcaaatga
    19501 actgaattat ttagaaagag aattacaaaa aacatcagcc gaatttgaag agttcaaaaa
    19561 agctcaagtt gaagctcaaa gaatggcaga aagtggctgg ggaaaaacca gtaaagtttt
    19621 tgaaagtatg ggacctaaat taacaaaaat gggtgatggt ttaaaatcca ttggtaaagg
    19681 catgatgatt ggtgtaactg cacctgtttt aggtatcgca gcagcatcag gaaaagcttt
    19741 tgcagaagtt gataaaggtt tagatactgt tactcaagca acaggcgcaa caggcagtga
    19801 attaaaaaaa ttgcagaact catttaaaga tgtttatggc aattttccag cagatgctga
    19861 aactgttggt ggagttttag gagaagttaa tacaagatta ggttttacag gtaaagaact
    19921 tgaaaatgcc acagagtcat tcttgaaatt cagtcatata acaggttctg acggtgtgca
    19981 agccgtacag ttaattaccc gtgcaatggg cgatgcaggt atcgaagcaa gtgaatatca
    20041 aagtgttttg gatatggtag caaaagcggc gcaagctagt gggataagtg ttgacacatt
    20101 agctgatagt attactaaat acggcgctcc aatgagagct atgggctttg agatgaaaga
    20161 atcaattgct ttattctctc aatgggaaaa gtcaggcgtt aatactgaaa tagcattcag
    20221 tggtttgaaa aaagctatat caaattgggg taaagctggt aaaaacccaa gagaagaatt
    20281 taagaagaca ttagcagaaa ttgaaaagac gccggatata gctagcgcaa ctagtttagc
    20341 gattgaagca tttggtgcaa aggcaggtcc tgatttagca gacgctatta aaggtggtcg
    20401 ctttagttat caagaatttt taaaaactat tgaagattcc caaggcacag taaaccaaac
    20461 atttaaagat tctgaaagtg gctccgaaag atttaaagta gcaatgaata aattaaaatt
    20521 agtaggtgct gatgtatggg cttctattga aagtgcgttt gctccagtca tggaagaatt
    20581 aataaaaaag ctatcagtcg ctgtcgattg gttttcgaat ttaagtgatg gttctaaaag
    20641 atcaattgtt attttcggtg gtattgctgc tgcaattggt cctgtagttt ttgggttagg
    20701 tgcatttata agcacaattg gtaatgcagt aactgtatta gccccactat tagctggtat
    20761 tgcaaaggct gatggattaa ttagtttttt atcgactaaa gtacctatat taggaactgt
    20821 cttcacagct ttaactggtc caattggcat tgtattaggt gttttagctg gcctagcagt
    20881 cgcatttaca attgcttata agaaatctga aacatttaga aattttgtta atgatgcaat
    20941 tgaaagtgtt aaacaaacat ttagtaattt tattcaattt attcaacctt tcattgattc
    21001 cgttaaaaac gtctttaaac aagcggtttc agcaatcgtt gatttcgcta aagatatttg
    21061 gagtcaaatt aatggattct ttaatgaaaa cggaatttct attgttcaag cgcttcaaaa
    21121 tatatgcaat tttatcaaag ttatatttga atttatctta aattttgtaa ttaaaccaat
    21181 aatgtttgca atctggcaag tgatgcaatt tatttggccg gcggttaaag ccttgattgt
    21241 cagtacttgg gagaatataa aaggtgtgat acaaggaact ttaaatatca tactaggttt
    21301 aattaagttc ttctcaagtt tatttactgg agattggcga ggagtttggg acgcgattat
    21361 tatgattctt aaaggagtcg ttcaattaat atggaattta attcaattat ggtttgtagg
    21421 caaaatactt ggcgttgtta ggtactttgg cggattgcta aaaggattaa taacaggtat
    21481 ttgggacgta ataaaaagta tattcagtaa atctttatca gcaatttgga atgcgacaaa
    21541 aagtattttt ggattcttat ttaatagtgt caaatcaatt ttcacgaata tgaaaaattg
    21601 gttatctaac acatggagta gtatccgtac gaatacgata ggaaaagcac agtcattatt
    21661 tagtggtgtg aaatcaaaat tcactagttt gtggaatgcg acaaaagata tttttagtaa
    21721 tctaagaaat tggatggcaa atatttggaa ttccattaaa gataatacgg ttggaattgc
    21781 aagtcgttta tggagtaaag tgcgtggaat ttttacaaat atgcgtgacg gcttacaaag
    21841 tattatcagc aaaattaaaa gtcatatcgg cggtatggta gatgctatta aaaaaggact
    21901 taataaatta attgaaggtt taaactgggt cggtggtaag ctgggaatgg ataaaatacc
    21961 taagttacac actggtacag aacacacaca tactactaca agattagtta agaacggtaa
    22021 gattgcgcgg gatacgttcg ctacggttgg ggataaagga cgtggaaatg gtccgaatgg
    22081 tttcagaaat gaaataattg aattccctaa tggtaaacgt gtaatcacac ctaatacaga
    22141 tactaccgct tatttaccta aaggctcaaa agtatacaac ggtacacaaa cttattcaat
    22201 gttaaacgga acgcttccaa gatttagctt aggtactatg tggaaagata ttaaatccgg
    22261 tgcatcatcg gcatttaact ggacaaaaga tcaaataggt aaaggtacca aatggcttgg
    22321 cgataaagtt ggcgatgttt tagattttat ggaaaatcct ggcaaacttt taaattatat
    22381 acttgaagct tttggaattg atttcaattc tttaactaaa ggtatgggaa ttgcaggcga
    22441 cataacaaaa gctgcatggt ctaagattaa gaaaagtgct actgattgga taaaagaaaa
    22501 tttagaagct atgggcggtg gcgatttagt cggcggaata ttagaccctg acaaaattaa
    22561 ttatcattat ggacgtaccg cagcttatac tgctgcaact ggaagaccat ttcatgaagg
    22621 tgtcgatttt ccatttgtat atcaagaagt tagaacgccg atgggtggca gacttacaag
    22681 aatgccattt atgtctggtg gttatggtaa ttatgtaaaa attactagtg gagttatcga
    22741 tatgctattt gcgcatttga aaaactttag caaatcacca cctagtggca cgatggtaaa
    22801 gcccggcgat gttgttggtt taactggtaa taccggattt agtacaggac cacacttaca
    22861 ttttgaaatg agaagaaatg gacgtcactt tgaccctgaa ccatatttaa gaaatgcaaa
    22921 gaaaaaaggt aggttatcaa ttggtggcgg tggtgctact tctggaagtg gtgcaactta
    22981 tgcaagccga gtaatccgtc aagcgcaaag tattttagga ggacgttata aaggtaagtg
    23041 gattcatgac cagatgatgc gagttgcaaa gcgtgaaagt aactatcaat caaatgcagt
    23101 gaataattgg gatattaatg ctcaaagagg agacccatca agaggattat tccaaatcat
    23161 cggctcaact tttagagcaa acgctaaacg tggatatact aactttaata atccggttca
    23221 tcaaggtatt tcagcaatgc agtacattgt tagacgctac ggttgggatg gatttaagcg
    23281 tgctggtgat tacgcatatg ctacaggtgg aaaagttttt gatggttggt ataacttagg
    23341 tgaagacggt catccagaat ggattattcc aacagatcca gctcgtagaa atgatgcaat
    23401 gaagatgttg cattatgcag cagcagaagt aagagggaga aaagcgagta aaaataagcg
    23461 tcctagtcaa ttgtctagtg taaatgggtt tgatgaccca agcttattat tgaaaatgat
    23521 tgaacaacag caacaacaaa tagctttatt actgaaaata gcgcaatcca acgatgtgat
    23581 tgcagataaa gattatcagc cgattattga cgaatacgct tttgataaaa aggtgaacgc
    23641 gtctatagaa aagcgagaaa ggcaagaatc aacaaaagta aagtttagaa aaggaggaat
    23701 tgctattcaa tgatagacac tattaaagtg aacaacaaaa caattccttg gttgtatgtc
    23761 gaaagagggt ttgaaatacc ctcttttaat tatgttttaa aaactgaaaa tgtagatgga
    23821 cgttcggggt ctatatataa agggcgtagg cttgaatctt atagttttga tatacctttg
    23881 gtggtacgta atgactattt atctcacaac ggctttaaaa cacatgatga cgtcttgaat
    23941 gaattagtaa agttttttaa ctacgaggaa caagttaaat tacaattcaa atctaaagat
    24001 tggtactgga acgcttattt tgaaggacca ataaagctgc acaaagaatt tgcaatacct
    24061 gttaagttca ctatcaaagt agtactaaca gacccttaca aatattcagt aacaggatat
    24121 aaaaatactg cgatttcaga ccaagtttca gttgtaaata gtgggactgc tgacactcct
    24181 ttaattgttg aagcccgagc aattaaacca tctagttact ttatgatcac taaaaatgat
    24241 gaagattatt ttatggttgg tgatgatgag gtaaccaaag aagttaagga ttacatgcct
    24301 cctgtttatc atagtgagtt tcgtgatttc aaaggttgga ctaagatgat tactgaagat
    24361 attccaagta atgatttagg tggtaaagtc ggcggtgact ttgtgatatc caatcttggc
    24421 gaaggatata aagcaactaa tttttctgat gcaaaaggtt gggttggtgc tggcacgaaa
    24481 cgagggctcc ctaaagcgat gacagatttt caaattacct ataaatgtat tgttgaacaa
    24541 aaaggtaaag gtgccggaag aacagcacaa catatttatg atagtgatgg taagttactt
    24601 gcttctattg gttatgaaaa taaatatcat gatagaaaaa taggacatat tgttgttacg
    24661 ttgtataacc aaaaaggaga ccccaaaaag atatacgact atcagaataa accgataatg
    24721 tataacttgg acagaatcgt tgtttatatg cggctcagaa gagtaggtaa taaattttct
    24781 attaaaactt ggaaatttga tcacattaaa gacccagata gacgtaaacc tattgatatg
    24841 gatgagaaag agtggataga tggcggtaag ttttatcagc gtccagcttc tatcatagct
    24901 atctatagtg cgaagtataa tggttataag tggatggaga tgaatggatt aggttcattc
    24961 aatacggaga ttctaccaaa accgaaaggc gcaagggatg tcattataca aaaaggtgat
    25021 ttagtaaaaa tagatatgca agcaaaaagt gttgtcatca atgaggaacc aatgttgagc
    25081 gagaaatcgt ttggaagtaa ttatttcaat gttgattctg ggtacagtga attaatcata
    25141 caacctgaaa acgtctttga tacgacggtt aaatggcaag atagatattt atagaaagga
    25201 gatgagagtg tgatacatgt tttagatttt aacgacaaga ttatagattt cctttctact
    25261 gatgaccctt ccttagttag agcgattcat aaacgtaatg ttaatgacaa ttcagaaatg
    25321 cttgaactgc tcatatcatc agaaagagct gaaaagttcc gtgaacgaca tcgtgttatt
    25381 ataagggatt caaacaaaca atggcgtgaa tttattatta actgggttca agatacgatg
    25441 gacggctaca cagagataga atgtatagcg tcttatcttg ctgatataac aacagctaaa
    25501 ccgtatgcac caggcaaatt tgagaaaaag acaacttcag aagcattgaa agatgtgttg
    25561 agcgatacag gttgggaagt ttctgaacaa accgaatacg atggcttacg tactacgtca
    25621 tggacttctt atcaaactag atatgaagtt ttaaagcaat tatgtacaac ctataaaatg
    25681 gtattggatt tttatataga gcttagttct aataccgtca aaggtagata tgtggtactc
    25741 aaaaagaaaa acagcttatt caaaggtaaa gaaattgagt atggtaaaga tttggttggg
    25801 ttaactagga agattgatat gtcagaaatc aaaacagcat taattgctgt gggacccgaa
    25861 aatgacaaag gaaagcgttt agagctagtt gtgacagatg acgaagcgca aagtcaattc
    25921 aacctaccta tgcgctatat ttgggggata tatgaaccac aatcagatga tcaaaatatg
    25981 aatgaaacac gattaagttc tttagccaaa acagagttaa ataaacgtaa gtcggcagtt
    26041 atgtcatatg agattacttc tactgatttg gaagttacgt atccgcacga gattatatca
    26101 attggcgata cagtcagagt aaaacataga gattttaacc cgccattgta tgtagaggca
    26161 gaagttattg ctgaagaata taacataatt tcagaaaata gcacatatac attcggtcaa
    26221 cctaaagagt tcaaagaatc agaattacga gaagagttta acaagcgatt aaacctaata
    26281 caccaaaaat taaacgacaa tattagcaat atcaatacta tagtaaaaga tgttgtagat
    26341 ggtgaattag aatactttga acgcaaaatt cataaaagtg atacaccgcc agaaaatcca
    26401 gtcaatgata cgctttggta tgatacaagt aaccctgatg ttgctgtctt gcgtagatat
    26461 tggaatggtc gatggattga agcaacacca aatgatgttg aaaaattagg tggtataaca
    26521 agagagaaag cgctattcag tgaattaaac aatattttta ttaatttatc tatacaacac
    26581 gctagtcttt tgtcagaagc tacagaatta ctgaatagcg agtacttagt agataatgat
    26641 ttgaaagcgg acttacaagc aagtttagac gctgtgattg atgtttataa tcaaattaaa
    26701 aataatttag aatctatgac acccgaaact gcaacgattg gtcggttggt agatacaaaa
    26761 actttatttc ttgagtatag aaagaaatta caagatgttt atacagatgt agaagatgtc
    26821 aaaatcgcca tttcagatag atttaaatta ttacagtcac aatacactga tgaaaaatat
    26881 aaagaagcgt tggaaataat agcaacaaaa tttggtttaa cggtgaatga agatttgcag
    26941 ttagtcggag aacctaatgt tgttaaatca gctattgaag cagctagaga atccacaaaa
    27001 gaacaattac gtgactatgt aaaaacatcg gactataaaa cagacaaaga cggtattgtt
    27061 gaacgtttag atactgctga agctgagaga acgactttaa aaggtgaaat caaagataaa
    27121 gttacgttaa acgaatatcg aaacggattg gaagaacaaa aacaatatac tgatgaccag
    27181 ttaagtgatt tgtccaataa tcctgagatt aaagcaagta ttgaacaagc aaatcaagaa
    27241 gcgcaagaag ctttaaaatc atacattgat gctcaagata atcttaaaga gaaggaatcg
    27301 caagcgtatg ctgatggtaa aatttcggaa gaagagcaac gcgctataca agatgctcaa
    27361 gctaaacttg aagaggcaaa acaaaacgca gaactaaagg ctagaaacgc tgaaaagaaa
    27421 gctaatgctt atacagacaa caaggtcaaa gaaagcacag atgcacagag gagaacactg
    27481 actcgctatg gttctcaaat tatacaaaat ggtaaggaaa tcaaattaag aactactaaa
    27541 gaagagttta atgcaaccaa tcgtacactt tcaaatatat taaacgagat tgtccaaaac
    27601 gttacagatg gaacaacaat cagatatgat gataacggag tggctcaagc tttaaatgtg
    27661 gggccacgtg gtattagatt aaatgctgat aaaattgata ttaacggtaa tagagaaata
    27721 aaccttctta tccaaaatat gcgagataaa gtagataaaa ccgatattgt caacagcctt
    27781 aatttatcaa gagagggtct tgatatcaat gttaatagaa ttggaattaa aggcggtaac
    27841 aataacagat atgttcaaat acagaatgat tctattgaac taggtggtat tgtgcaacga
    27901 acttggaaag gcaaacgatc aaccgatgat atattcacac gtcttaaaga tggacatcta
    27961 aggtttagaa ataataccgc aggcggttca ctttatatgt cacattttgg tatttcaaca
    28021 tatattgatg gagaaggcga agacggaggt tcatccggta ctattcaatg gtgggataaa
    28081 acttacagtg atagcggtat gaatggcata acaatcaatt cctatggtgg tgtcgttgca
    28141 ctaacgtcag ataataatcg ggttgttctg gagtcttacg cttcatcgaa tatcaaaagc
    28201 aaacaggcac cggtgtattt atatccaaac acagacaaag tgcctggatt aaaccgattt
    28261 gcattcacgc tgtctaatgc agataatgct tattcgagtg acggttatat tatgtttggt
    28321 tctgatgaga actatgatta cggtgcgggt atcaggtttt ctaaagaaag aaataaaggt
    28381 cttgttcaaa ttgttaatgg acgatatgca acaggtggag atacaacaat cgaagcaggg
    28441 tatggcaaat ttaatatgct gaaacgacgt gatggtaata ggtatattca tatacagagt
    28501 acagacctac tgtctgtagg ttcagatgat gcaggagata ggatagcttc taactcaatt
    28561 tatagacgta cttattcggc cgcagctaat ttgcatatta cttctgctgg cacaattggg
    28621 cgttcgacat cagcgcgtaa atacaagtta tctatcgaaa atcaatataa cgatagagat
    28681 gaacaactgg aacattcaaa agctattctt aacttaccta ttagaacgtg gtttgataaa
    28741 gctgagtctg aaattttagc tagagagctg agagaagata gaaaattatc ggaagacacc
    28801 tataaacttg atagatacgt aggtttgatt gctgaagagg tggagaattt aggattaaaa
    28861 gagtttgtca cgtatgatga caaaggagaa attgaaggta tagcgtatga tcgtctatgg
    28921 attcatctta tccctgttat caaagaacaa caactaagaa tcaagaaatt ggaggagtca
    28981 aagaatgcag gataacaaac aaggattaca agctaatcct gaatatacaa ttcattattt
    29041 atcacaggaa attatgaggt taacacaaga aaacgcgatg ttaaaagcgt atatacaaga
    29101 aaataaagaa aatcaacaat gtgctgagga agagtaatct ttagcactat ttttatacaa
    29161 aaatttaagg aggtcattta attatggcaa aagaaattat caacaataca gaaaggttta
    29221 ttttagtaca aatcgacaaa gaaggtacag aacgtgtagt atatcaagat ttcacaggaa
    29281 gttttacaac ttctgaaatg gttaaccatg ctcaagattt taaatctgaa gaaaacgcta
    29341 agaaaattgc ggagacgtta aatttgttat atcaattaac taacaaaaaa caacgtgtga
    29401 aagtagttaa agaagtagtt gaaagatcag atttatctcc agaggtaaca gttaacactg
    29461 aaacagtatg aaaagctatg agttagatac tcataatctt tattctttta gaaagcgggt
    29521 gtactgaatt ggggtggttc aaaaaacacg aacatgaatg gcgcatcaga aggttagaag
    29581 agaatgataa aacaatgctc agcacactca atgaaattaa attaggtcaa aaaacccaag
    29641 agcaagttaa cattaaatta gataaaacct tagatgctat tcaaaaagaa agagaaatag
    29701 atgaaaagaa taagaaagaa aatgataaga acatacgtga tatgaaaatg tgggtgcttg
    29761 gtttagttgg gacaatattt ggatcgctaa ttatagcatt attgcgtatg cttatgggca
    29821 tataagagag gtgaataaaa tgtttaaact aatctttggt tatagtttct ggacatgttt
    29881 ttggttcggt aaatgtaaat aagttttagt cagtgcttcg gtactgactt tttatttatt
    29941 gttgtaatta tggtaatatg cagaagtgag caagttggat agatggtggc tatctgagta
    30001 taaggaggtg gtgcctatgg tggcattact gaaatcttta gaaaggagac gcctaatgat
    30061 tacaattagt accatgttgc agtttggttt attccttatt gcattgatag gtctagtaat
    30121 caagcttatt gaattaagca ataaaaaata accatcgcta actttggctg gtttcgatgg
    30181 ttaaatggtt attaatttaa tctttaatct aaaatagcca ccgtcttttt aacgggctca
    30241 ttagggtaac atgtttgcgc atgttgccct ttttctatat ataaattaac acaccataat
    30301 ataaatatca aatagacggc ttattagtcg tctttttatt ttgggtaaaa ggagataaga
    30361 atatgattaa ttggaaaatt agaatgaaac aaaaatcatt ttgggtagcg atattgtcag
    30421 ctatcttttt atttgctcaa aacatcgcaa aagctattgg gtatgatatc caagtttata
    30481 cagagcaatt aacagacggt ttaaacgcta tattaggatt tttagtatta actggtgtga
    30541 ttcaagaccc gactactaaa ggtataggtg atagccacca agctttagaa tatgaagaac
    30601 caagaagaaa atactaggag gtaaaataat gaaaacatac agtgaagcaa gagcaaggtt
    30661 acgttggtat caaggtagat atattgattt tgacggttgg tatggttacc aatgtgcaga
    30721 tttagcagtt gattacattt attggttgtt agaaattaga atgtggggaa atgcaaaaga
    30781 tgcaatcaat aacgatttta aaaacatggc aacagtatat gaaaacacac catcgtttgt
    30841 tccacaaata ggtgatgtgg ctgtatttac caaaggaata tataaacaat acggtcatat
    30901 tggtttagtg tttaatggtg gtaatacaaa ccaattttta attttggaac agaactatga
    30961 cggtaacgca aatacgcctg caaagttacg ttgggataat tattacggct gtactcactt
    31021 tattagacct aagtataaaa gtgagggctt aatgaataag atcacaaata aagttaaacc
    31081 acctgctcaa aaagcagtcg gtaaatctgc aagtaaaata acagttggaa gtaaagcgcc
    31141 ttataacctt aaatggtcaa aaggtgctta ttttaatgcg aaaatcgacg gcttaggtgc
    31201 tacttcagcc actagatacg gtgataatcg tactaactat agattcgatg ttggacaggc
    31261 tgtatacgcg cctggaacat taatatatgt gtttgaaatt atagatggtt ggtgtcgcat
    31321 ttattggaac aatcataatg agtggatatg gcatgagaga ttgattgtga aagaagtgtt
    31381 ttaattctta ggttaaaatg ttaaatattt gttaattatt ttttaatgta attttagttt
    31441 cttttaatat tttattgatt tttaatattt tctcaatata aaatgaagtt gttgatattt
    31501 atcatcttaa ataagggtgt tagctataaa aagagataaa taaaaacaaa tatattatat
    31561 ttggaggaag cgccatgctc aaaagaggtt tattattttt aactgtttta ttgttattat
    31621 tctcattttc ttcaattact aatgaggtaa gtgcatcaag ttcattcgac aaaggaaaat
    31681 ataaaaaagg cgatgacgcg agttattttg aaccaacagg cccgtatttg atggtaaatg
    31741 tgactggagt tgatggtaaa ggaaatgaat tgctatcccc tcattatgtc gagtttccta
    31801 ttaaacctgg gactacactt acaaaagaaa aaattgaata ctatgtcgaa tgggcattag
    31861 atgcgacagc atataaagag tttagagtag ttgaattaga tccaagcgca aagatcgaag
    31921 tcacttatta tgataagaat aagaaaaaag aagaaacgaa gtctttccct ataacagaaa
    31981 aaggttttgt tgtcccagat ttatcagagc atattaaaaa ccctggattc aacttaatta
    32041 caaaggttat tatagaaaag aaataaaaca aaatagttgt ttattataga aagcaatgtc
    32101 ttgattgaat atgtgtagtg aaaattatct ttcatcaaat tctcattcat gcacgaatgg
    32161 ttcttcccca cctaatcaga tattaggtga cttatgggga gaaatcagtt aggatgaaaa
    32221 agtggataat ccttttttag gcaggtactt cggtacttgc ctattttttt atgttataat
    32281 ctttctagac gtattcaagg gacgtctttt tagattgtat gttatagcta gctttcgggc
    32341 tagttttttg ttatgatgtg ttacacatgc atcaactatt tacatctatc cttgttcacc
    32401 caagcatgtc actgggtgtt ttttcttatg atagagagca tagttttcat actactccct
    32461 cgtagtatat atgactttag cattcccgta taatagttta cggggtgctt tttatgttat
    32521 aattaactgt atatagtagg agtgaactat atagcctgtt aagtggccta gtaacctaac
    32581 acttatcctg caattgatat cctttttgcc cttcactcga tacatatatc tcaacaacat
    32641 agaaatatta cagtcgctac accgcatctt aaatggtgtg gttattttta ttggaagtgt
    32701 gtatcaggta tcagtaatgt taaaacacca gctaaaaatg aaaagaattc accagtgcca
    32761 gcaggttata cactcgataa aaacaatgta ccgtataaaa aagagactgg ttattacaca
    32821 gttgccaatg ttaaaggtaa taacgtgagg gatggctatt caactaattc aagaattaca
    32881 ggtgtattac ccaataacgc aactatcaaa tatgacggcg catattgcat taatggctat
    32941 agatggatta cttatattgc taatagtgga caacgtcgtt atatagcgac aggagaggta
    33001 gacaaggcag gtaatagaat aagcagtttt ggtaagttta gtgcagtttg ataattagat
    33061 atataaaggt ttggcaagtt atgaaatgtc tgccaaacct ttatataaaa aagaaatatc
    33121 taccttttaa tttatgtaac tactattagt atgcatattc attagttttt ccaggaccat
    33181 taattacata agatgattta gactctcctt ttttaaagaa gtatgtttta tacattttac
    33241 ctagtaactc aacatttttt ctatcttcag caagtggtgt attcagatat actgtatagt
    33301 aacctttatt ttcagttaaa ataaccattt tttcaaattg agcagaattt tttgtgcctt
    33361 tctttaaata atttctcaaa cgttcatcta attttcctag cgttgtagga agaccactat
    33421 ttttaaatga ttctttataa gctttttctt tctctaacat tttcttattt gattctattt
    33481 cttcatttgt aggaaacggt tcaaaagtaa aagctttcgc tgaatgatgg tgtgtactga
    33541 ttcctgccgt taaaaaactt aatgctaaaa ctgttgttgc taatttcttt ttcataatga
    33601 tgttaattct ccttaaattc tatatttaaa tttatagtta gttttgcgaa attcctaaaa
    33661 tgagtttaat ctaatcgacg aaatatatta attaacttga aattaataaa agattaatta
    33721 ttttt
//
LOCUS       DALAGF010000018        33527 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.018, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000018 DALAGF010000000
VERSION     DALAGF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33527
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.018"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..925)
                     /locus_tag="O2692_000811"
     CDS             complement(<1..925)
                     /locus_tag="O2692_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HCZ4298449.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQII"
     gene            complement(1275..1637)
                     /locus_tag="O2692_000812"
     CDS             complement(1275..1637)
                     /locus_tag="O2692_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCZ4298450.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
     gene            complement(1634..1804)
                     /gene="mazE"
                     /locus_tag="O2692_000813"
     CDS             complement(1634..1804)
                     /gene="mazE"
                     /locus_tag="O2692_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCZ4298451.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            complement(1889..3037)
                     /gene="alr"
                     /locus_tag="O2692_000814"
     CDS             complement(1889..3037)
                     /gene="alr"
                     /locus_tag="O2692_000814"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCZ4298452.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            complement(3103..3462)
                     /locus_tag="O2692_000815"
     CDS             complement(3103..3462)
                     /locus_tag="O2692_000815"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCZ4298453.1"
                     /translation="MIHGIGVDLIEIDRIQALYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            complement(3466..3957)
                     /locus_tag="O2692_000816"
     CDS             complement(3466..3957)
                     /locus_tag="O2692_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCZ4298454.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNHLSIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            complement(3944..5527)
                     /locus_tag="O2692_000817"
     CDS             complement(3944..5527)
                     /locus_tag="O2692_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCZ4298455.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            complement(5520..5999)
                     /locus_tag="O2692_000818"
     CDS             complement(5520..5999)
                     /locus_tag="O2692_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298456.1"
                     /translation="MSDYTKLTKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLFIKPLWQYHCRFYKVDQLSVQYRTSFFIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEERTMSNLRGVESDV"
     gene            complement(6207..6767)
                     /locus_tag="O2692_000819"
     CDS             complement(6207..6767)
                     /locus_tag="O2692_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCZ4298457.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHVSTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            complement(6787..8814)
                     /gene="kdpB"
                     /locus_tag="O2692_000820"
     CDS             complement(6787..8814)
                     /gene="kdpB"
                     /locus_tag="O2692_000820"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCZ4298458.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNLKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            complement(8833..10509)
                     /gene="kdpA"
                     /locus_tag="O2692_000821"
     CDS             complement(8833..10509)
                     /gene="kdpA"
                     /locus_tag="O2692_000821"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HCZ4298459.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            complement(10533..10643)
                     /gene="kdpF"
                     /locus_tag="O2692_000822"
     CDS             complement(10533..10643)
                     /gene="kdpF"
                     /locus_tag="O2692_000822"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCZ4298460.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            10781..13438
                     /locus_tag="O2692_000823"
     CDS             10781..13438
                     /locus_tag="O2692_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HCZ4298461.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVNLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SYEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIYTDFNEGDV"
     gene            13438..14133
                     /locus_tag="O2692_000824"
     CDS             13438..14133
                     /locus_tag="O2692_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4298462.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(14502..16022)
                     /locus_tag="O2692_000825"
     CDS             complement(14502..16022)
                     /locus_tag="O2692_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCZ4298463.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(16539..17897)
                     /locus_tag="O2692_000826"
     CDS             complement(16539..17897)
                     /locus_tag="O2692_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCZ4298464.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLIYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLINDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
     gene            complement(17912..18982)
                     /locus_tag="O2692_000827"
     CDS             complement(17912..18982)
                     /locus_tag="O2692_000827"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCZ4298465.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            19300..20502
                     /locus_tag="O2692_000828"
     CDS             19300..20502
                     /locus_tag="O2692_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HCZ4298466.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            complement(20766..20903)
                     /locus_tag="O2692_000829"
     CDS             complement(20766..20903)
                     /locus_tag="O2692_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298467.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(21031..21240)
                     /locus_tag="O2692_000830"
     CDS             complement(21031..21240)
                     /locus_tag="O2692_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HCZ4298468.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVF"
     gene            complement(21252..21545)
                     /gene="csoR"
                     /locus_tag="O2692_000831"
     CDS             complement(21252..21545)
                     /gene="csoR"
                     /locus_tag="O2692_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HCZ4298469.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            21711..23195
                     /gene="cls"
                     /locus_tag="O2692_000832"
     CDS             21711..23195
                     /gene="cls"
                     /locus_tag="O2692_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCZ4298470.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            23223..23870
                     /locus_tag="O2692_000833"
     CDS             23223..23870
                     /locus_tag="O2692_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCZ4298471.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNCHD"
     gene            complement(24683..25555)
                     /gene="yidC"
                     /locus_tag="O2692_000834"
     CDS             complement(24683..25555)
                     /gene="yidC"
                     /locus_tag="O2692_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCZ4298472.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(25641..26282)
                     /locus_tag="O2692_000835"
     CDS             complement(25641..26282)
                     /locus_tag="O2692_000835"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCZ4298473.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            complement(26284..27075)
                     /gene="thiM"
                     /locus_tag="O2692_000836"
     CDS             complement(26284..27075)
                     /gene="thiM"
                     /locus_tag="O2692_000836"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCZ4298474.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVIT
                     GKEDVIVQDNKAFVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            complement(27059..27889)
                     /gene="thiD"
                     /locus_tag="O2692_000837"
     CDS             complement(27059..27889)
                     /gene="thiD"
                     /locus_tag="O2692_000837"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCZ4298475.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            complement(27882..28571)
                     /gene="tenA"
                     /locus_tag="O2692_000838"
     CDS             complement(27882..28571)
                     /gene="tenA"
                     /locus_tag="O2692_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HCZ4298476.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     regulatory      complement(28651..28753)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(28957..29652)
                     /gene="sceD"
                     /locus_tag="O2692_000839"
     CDS             complement(28957..29652)
                     /gene="sceD"
                     /locus_tag="O2692_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HCZ4298477.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGTNGADMSAQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKSSEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     gene            complement(30089..30436)
                     /locus_tag="O2692_000840"
     CDS             complement(30089..30436)
                     /locus_tag="O2692_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002466750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCZ4298478.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSI"
     gene            30630..31070
                     /locus_tag="O2692_000841"
     CDS             30630..31070
                     /locus_tag="O2692_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HCZ4298479.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            complement(31128..31568)
                     /gene="fabZ"
                     /locus_tag="O2692_000842"
     CDS             complement(31128..31568)
                     /gene="fabZ"
                     /locus_tag="O2692_000842"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCZ4298480.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(31602..32867)
                     /gene="murA"
                     /locus_tag="O2692_000843"
     CDS             complement(31602..32867)
                     /gene="murA"
                     /locus_tag="O2692_000843"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCZ4298481.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRDAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            complement(32978..33211)
                     /locus_tag="O2692_000844"
     CDS             complement(32978..33211)
                     /locus_tag="O2692_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCZ4298482.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            complement(33343..33435)
                     /locus_tag="O2692_000845"
     CDS             complement(33343..33435)
                     /locus_tag="O2692_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298483.1"
                     /translation="MLKSKFPSPLSTVDISFFEIIYIFSYQDIL"
BASE COUNT        10770 a         6097 c         4944 g        11716 t
ORIGIN      
        1 agataatttg aacaatatct tgtgggtgca tatgtttatg ttttttaata tattctaaaa
       61 gtttttgttt atctataaag gtaccttcac tatttctagc ttcagtcaca ccatccgtta
      121 aaatgataat taaatcatca aggtatattg gaatttcttg ttgttgatat cgtgtttgtg
      181 aactgattcc taacactcta cctctaactg aaatttcttc aaattcttct ttttcagcgc
      241 gataaatata tccaggctca tgaccagctg aactacaata caataaatgg ttcatttctt
      301 catataaacc ataaaacatt gtgacgaaca tattttgatt aatattcttt tcaacaacac
      361 gatttaaacg ttttaaacca tcactcggta attgtgagtg tccataagaa tccatgccaa
      421 actttatcat actcattgct aaagcagctg gtataccttt tccaatgaca tctgcaacag
      481 caaagctcat tgtgccatcg ttatggtcaa ttaaattaaa ataatctcca cttacttttt
      541 gtgccgccac tgaaataacg ccaatttgaa tactatcaaa ttgtggaata tctgttttaa
      601 gcattgtttg ttgtaagcta gaagctaagt ctatctcttt atcgtgaact tgaagtttat
      661 ctaccaatct ttgataatct cgataactat aaccaaagcc tttaacgatt tcttgtaaga
      721 catctaatgt cttgaacaca tcttcacgcg ttaagtttaa cgtcaatata tagtttttgt
      781 gaatatcgac aatgtcttca ggcaagacgt ccttacgaat cacttcgtca gtgtatttct
      841 cacacttttt tatcaattct actttatctt ggcacgttaa actttcatca attaaaccct
      901 tataatgttg cttaaattct tccacgatta gttgcctcct cacttccctt acaaagcaaa
      961 tgcttaagca aggataatta tgattcactt actaacattt atttaattgc acttatcaat
     1021 tttgtcgttt gcaattttgc tgtagatgat acttttaagc taaaaaaaac tttaggatgg
     1081 aatacctaaa gcctaaaaaa cttaagacat ccttttataa taaaaaatat gaatatcata
     1141 actggtattt gctaaaatct ttgtttataa gaaaattctt cgtacagaat ttagaaatag
     1201 ttataattat tataaagtta ttgtctttta tatcaatatt ttatttatct ctgaaaaata
     1261 catataatag acgcctaatt tttctggtga gctactgcat tcagccctaa actaatcatt
     1321 agtgcattat ctacttcttt cattttatca tcggataagt acgtcagttt ttctttcaat
     1381 cgttttttat caagtgtacg aatttgttct aataatataa ctgagtcttt atccaactta
     1441 tactttttct tttcaatctc tacatgtgtc ggtattttcg ctttattaat cctaccagtt
     1501 attgccgcaa caataactgt aggactatat ttattaccag tatcattttg aattatgact
     1561 acaggtctga ctcccccttg ttcagatccc tgtactggtg ataaatctgc taaataaaca
     1621 tctcctcgtc taatcattca ttcgttgaat tagaagataa atatgtttcg ttgcaatcgc
     1681 atgcttcaca ctctatcgga aaagcttcgt tcgctaggga gagatttaaa tcagccattt
     1741 gtgaatatcc ttcttttaaa gattgttcta agctatgact tctattttga ctaaaagata
     1801 acatatgaga acctccaatt gattccaaaa gttattaaat gacctttaat tactctaatg
     1861 ataacaaatt ttatatgcac tgaccatatt attttaacaa ttcatttgtt acaaaacgtt
     1921 gatcaccatc atgatagatt cgcggcaaac gtctcgacaa gttacaaagc acttcataat
     1981 taatagtatg ttgcttttca gctaccacct ctaccgactg tggactttct ctatgattat
     2041 ctattaaaat caccgaatct ccagctttaa cttgatctgg cactttaaca attgtctgat
     2101 ccatacatac gcgaccaata acttcgcatt gatgaccatt tacatttaca aagctacctt
     2161 gcattatgcg taaatagcca tctgcatatc caataggtaa caatgctatt gtagttgggt
     2221 cagtagctgt ataagttgca ccataactta cagactcacc cgcttgtagc gtctttgttt
     2281 gaactacatt agcaattaat tgcacacttg gtttaaggtg tactttaact ttttgctgta
     2341 catactctga tggataatat ccataaaggg aaattcctgg tcttattgca ttacagaatt
     2401 ggcaatccat taatagagag cctgctgagt tctgacaatg tatatattca ggtttaattg
     2461 cttcattgac catatcttta aaacgttgat attgttcagt tgtcatatct cctggttcgt
     2521 cagcacaggc aaagtgtgta aacacgcctt caaatacaag ttgctcatat tgttgaatga
     2581 tttcaatcac ttcttgatac gttttagtat ctttaatacc taaacgtccc attcctgtat
     2641 ctaatttaat gtgcaaccat aactttttct cttgctcacc agaaatgttt ttaattgctt
     2701 ctttcaacca ctgtttagac ggaaccgtta aggcaactcg gtgttgtatc gctttatcaa
     2761 tatctttagc tggtaacaca cctaagacta aaattttagc agtaatccca tgcattctaa
     2821 gttctatcgc ttcatctaac gttgctacag caaaaaatgt ggcgccattt tccattaaat
     2881 gacgtgctac tttaacacta cctagtccat aggcattggc tttaacgaca gccatcactg
     2941 ttttatttgg atgcaatgta ctgaatactt tgaaatttga tgcaacagcg tttaaatcta
     3001 cattcatata cgcagatcta taatatttat ccgacatatt acttcctcct gtaattccca
     3061 cacgttttaa aactagatct taattatcat tgtataacaa atttaaaatg ctgacttttc
     3121 taaaacaact tggctcatcg cataatgctc agtgtgtgag atactaacat gtacgataaa
     3181 cccttcgtaa tcaatctttg gtttgccaag ttcgtcgttg taacagtcta tatcgttaaa
     3241 agctacatgt tttcctaagc ctgtgcctaa tgctttactg aacgcttctt ttgtagcaaa
     3301 cctgccagct aaaaattcaa ttttacgttg ctcatgtgtg aaattgttga atttgtgctg
     3361 ttcattttta gttaaaatcc gctcaaccaa ttttggttgc ttactatata acgcttgtat
     3421 tcgatcgatt tcgattaaat ctacaccaat tccatgtatc attgattatt cctcattttt
     3481 attgttttct aaatgtctct cgttatttaa ccatcgtttc aatctacgtg cttcacttct
     3541 atctattaat tcaatttcag cttgaccacc cgctgtataa agggtcaaag ttgctaatcg
     3601 atagcgtctc attataggac caacatcgat atcaatattt tgaatacgaa aatatggtat
     3661 taccttttca tccaaaaata aaatgccgtt tcgtacacgc aaatggtgtt tttcaaatgc
     3721 atatctgcag tgcttatatc gatagactgg cgctataaca agcgtgaaaa cagcaacaag
     3781 taatattata atcacactac taacaatgga taaatggtta tctaaaatct cccaaaatag
     3841 ccagttcaaa atattaaatg cgattaaaag tacaagcgct atgggtatcc aaaacagcac
     3901 agcacttaac ctcatcactt ttttagcgtg tggtgacata aaattataat ccctcatcat
     3961 tttcacctct taaataccat gatttcaatt tatttgcatc ttcacttctt gcgtatttta
     4021 agttaatcga ttgggctccc acacctttag caacaataaa gctaaaatta tttaaattgt
     4081 tgcgtttaag taatgtatgt tgccaagtgt caaatcctat aatgtgatgc gctttaaaat
     4141 aataaatatt tcgtttcaat agctcgaaat tctggatagt aatttgttct tctgtcattt
     4201 taaaacccgc atgtttgaca taaagatatc ctttgatcac aaataaacca ataatgacta
     4261 ttgttataat cgtaaataac aataataatt gattccaaaa ataacagcct atacctgcca
     4321 tagctatgac aataatacta ggtattaaaa agtgtctgtg gaaacctgac aaaggcatac
     4381 cttcattaac ttgttgataa gataaatctg gtactaaatt ctggataatt tgatatgctt
     4441 tgtctcgttt aataaacggc aatatcggca cactacctga atcattgtca tcatcttcga
     4501 tttttttatt ttcaaaatca cttgtgatga taaaatgtat tgctgtgtag ccaaataatc
     4561 ttcgtatata tgactgacgt tcaacaacag cttgcaatcg tttcgttggt acagttactt
     4621 tcttcttgtt aaagagacca tattcaattt taagttgctc attttcaagc atcaccgaaa
     4681 agccataaaa tcttatcatt gttataatcg ttccaataat atatgccact attaatacta
     4741 gtaaaatgat gattaatact gaaatactta caatttgaac ccattgacta atttcatgat
     4801 ttagcttcga ccatgggatc aactctctta cagccccgta aatcggtact aaagctgcta
     4861 acgttacacc aatggcgcca ctggtcattg ccataaatag tgattcttta aaattcatct
     4921 gatatatagg aatgcgttta tttttctgat taagcatact atcagtgttc tgcacttcat
     4981 ctaagcgacc ttctgcgatg tcttccacat taccttcaat gtcatgatta cagttgtcat
     5041 tcttctcagc actagacttt tgcgccactt ctgtcttcaa ctctgtttgc aattgatcaa
     5101 tatatcgttc aagatattca ccttgttttt tcgaaataac acttaagaca ataccatcac
     5161 ttggtgtttt gatttgtaaa tccacaccac caataatttg attgacgacc ccttgtgtca
     5221 tatcaacgga ctgaatacgt tttatattta attctttacg ctttttatta aaaatacctg
     5281 ttgttaaaat gaaataatta tcctcaatcc aatatcgcgt gttcataatt ccgacaattt
     5341 gagaaatgta tgatattaaa aagaatacaa atacaatacc tatccataaa tatgattcgg
     5401 gattcgtata atcaaaatct ttcaattgaa agataatgaa aataaaaaag acgactatgt
     5461 tttgtttgat agcattgatt atgccattaa aatatgaaat cggatgtaat ttttgaggtt
     5521 cagacatcac tttcaacccc tctcaaattc gacatagttc tctcttcgat tattttaaca
     5581 tcgtcatgag acatcatcgg taaataaata gtatgacctg cagtcataaa tccaacttta
     5641 tacaaattaa gcactttact aattggatta gatttaatcg acaagtattg taaacgttca
     5701 attcgactcg tttcttcttt atatataaaa aatgatgtac gatattgtac acttagttga
     5761 tcaactttat aaaagcgaca atgatattgc cataaaggct taataaataa ttttaatgta
     5821 ctcagagcac ctaaaaccaa caaaatataa agtaagtaat gtggccattc aaatcttaac
     5881 catataaaat aaaaaatgac atacacagct acactcaata taaattctaa gccattcgta
     5941 atgtagtaat acaacaatgc tgacttagga ctcttagtca acttagtata atctgacata
     6001 tacccctctc cccaaataaa aaattatacg gatttataat ctatttcatt ttatttttat
     6061 atgatgataa ttatagcata tggaatattt catgctaatt tattcttcct aaaggtacat
     6121 ctaaaaattt aattaagcag aaagtgcttg aattgctaaa aagacaccat gtataatttt
     6181 atcaacatga tgcctttcat ctataatcaa tctttcatct tatcaagagc gatatttagt
     6241 tcaagcacat tcacataatc atttgttaac acaccacgct gcttacgatg ttgaatcaag
     6301 tcggccactc ttgaagtaga tacatgacga gcatcagcaa tacgaggtgc ttgcttcaat
     6361 gcattttcga ccgtaatatg cggatctaag cccgacccag aacttgttgc agcatctatt
     6421 gttacatttg aattcccaaa tttaacatga tgtttcatgc gtgctattaa ttcggtgttt
     6481 ccattcgatt cattacttcc acctgaagat acgccgtttt tatataattt ttcaggattc
     6541 atattataat caactgcact cggtctcccg tgaaaatatc gtgtctctgt ccagtgctgt
     6601 ccaatcaatt ttgatccaac tatacgattg tcatacgtaa ttaaactgcc atttgcttgt
     6661 tgataaaaaa atatttgacc aattaacgtg atagctaacg ggaataaaaa tccacataat
     6721 accatagtta ttatcgttaa acaaatacta tttcttatcg tattcatggt acaggctcct
     6781 tcctctttac acaaaaaatt gtacaatcat atctattaat ttaatgccta aaaacgggac
     6841 gattaatcca cctaatccat aaatcaacat attatttata aagattctat caatgctgta
     6901 accctttact tttacacctt tcatggcaat tggaattaag gcaacaatga ttaatgcatt
     6961 gaatatcaaa gctgatataa ttgcagattt aggtgacgat aaatgcataa tatttagtga
     7021 agtcatttct ggaattgttg acatcattag tgcaggtaaa attgcaaagt attttgctac
     7081 gtcattagcc aaactaaatg tcgttaatgc acctctcgtc attaataatt gtttgcctat
     7141 ttttacaacc tctattaact ttgtaggatt cgaatctaaa tcaattagat tagctgcctc
     7201 tttagcacta attgtccctg agttcatagc taatcctata ttcgcttgtg ctagcgcagg
     7261 tgcatcattt gtaccatctc ctgtcatcgc aacaatatgg cctttcgctt gttcatcttt
     7321 gatgacttta attttatctt cgggtttaca ctctgcaaca aatctatcaa ccccggcttc
     7381 ttttgcaatt gtagctgctg ttaaagcatt atcacctgta cacataactg tttcaatccc
     7441 catttttctc aattcagtaa atcgttctac aagaccatct ttaatcacat cttttaaata
     7501 aatcacgcca agcatgacat tgttttcaat gactattaat ggtgtgccac ctttactcga
     7561 tacatccata cagagagact caatattaag aggaatattg ccttgttgtt gtttgacaag
     7621 atttatcata ctattaggtg cacctttgaa taccgatatt tcatttgtaa tgattccgct
     7681 cattctagtt tcagctgtaa aaggcttata tgtgccatca atgtctttag gcagctcatt
     7741 tatatacatc tgcttcgcta atcgtacaat actttttcct tctggcgtat catcgtagat
     7801 tgatgacata taagcagcga ctatcaattt ttcaagcatt tgttgattca ctggtaaaaa
     7861 ttcactagcg attcgattgc cataagtgat tgtgcctgtc ttgtctaaaa tcattacatc
     7921 gacatctcca catacttcta cagcacgccc acttttcgct aatacattga attgagtaac
     7981 acgatccatg cctgcaatac caatcgccga taacaaacca ccgattgtcg ttggtattaa
     8041 acatactgtt aacgcaatga gcatcgcaat aggtaaaatt aaatgcaggt aagatgctat
     8101 tggatataac gttacaataa cgactaaaaa tataattgtt aacgttgtta ataatgtaaa
     8161 aagtgcaatt tcatttggtg ttttatttct ttccgcccct tcaactaagg caatcatttt
     8221 atctaaaaaa gatgtacccg cttcactctc aacacgtatt tctaaccaat cagatgttac
     8281 aagtgtaccg ccaatgactc catcaaaatc gccacctgat tcttttatca caggtgcaga
     8341 ctcaccagta attgcagatt catcaacggt tgctaatcca tttattacaa cgccatcagc
     8401 agggattgtt tctccatttt ctacccgaat attttgtccg gcttttaact ctgtggcgtt
     8461 cactatccga tacgcaccat tttcttctat caatcgagca gttaaatttg attgtgcttg
     8521 tcttaaacta tcagcttgcg cttttccacg accttcagca aaggcttctg aaaaattagc
     8581 aaacaatata gttattaata atatgataaa aattgtaatc aaataacctc gcgatagata
     8641 gctagttcca aatatgtcag gaaaacatat taatatcaac gttaaaatca ttccaacctc
     8701 aacgacaaac attatcggat tttttattaa ttgtttaaga ttcagcttat aaaaactcat
     8761 tttcaaagct tctatcacca ttgtttgatt gaaatattta tttacatgat gcattttttc
     8821 catcccttta ctttatttta aagttaaaaa ttcaccaata ggaccaagta atagtactgg
     8881 aataaatgtc aaaccactta gtaaaacgat aaatacgatt agtgatacgc caaaataagg
     8941 tttatcaatc gctattgtat atttatcttg atggtatgat tttttattca ctaaacttga
     9001 tgcaatcatt aattgcaaaa taattggtat ataacgagaa agcaacataa tgattcctgt
     9061 agagatattc cagaatgttg tatcatcttt cagtccttca aaccctgatc cattgttcgc
     9121 agcagctgat gtcatttcat acataacttg tgaaatacca tgaaaagacg gattcgttat
     9181 actttcactt gctccaggaa tcataaaagc aagtgctgaa aatactaaaa ttaaaattgg
     9241 gtgtatgaga aagactaaga caatacattt catttcacgg gcgccaattg gcatatttaa
     9301 atattctggt gttttaccaa ccatcaaact gcatataaac accgtcagta agacaaatat
     9361 caataaattc atgagtccta cgccttcgcc accaaataca acatttagca tcattaatac
     9421 cattggtcct aatccaccta taggcgttaa gctatcatgc atgttattaa cagaacccgt
     9481 tgtaaatgcc gtcgtaataa ctgtaaatag tgctgacaaa cctgctccaa accgtacctc
     9541 tttaccttcc atattcggtc cataaatgcc taaattcgct agtattggat taccacgata
     9601 ctcactccac atagttaatg taagaattgc tataaaaatg aaaaacattg cgacaaataa
     9661 tatcaacgca tgacgatgta ctcgtttacc atgtctactt aacatgcgac caaataagaa
     9721 caacattgac ataggaagta acatcatact gcccatttct ataaaattgc tccaaatatt
     9781 tggattttca aaaggtgtcg cagaatttcc tgctaaaaat cctccaccat tcgtaccaag
     9841 atgttttatt gattcaagtg atgcaatagg tccaaatgca atatgttgaa tatgtccgct
     9901 taaagtccga atcattaaat tagcatgcaa cgtttgtggt acaccttgag tcatcaataa
     9961 aatactaatt aaacatgata atggtaaaag tactcggaca ataaaccgaa caatatcttg
    10021 ataaaaatta ccaatgatat tagttaatcc agttaaacgt ctcaacatcg ctatacaaac
    10081 ggcgtaacct gatgcactag atgtaaacat taaatatgtc attacaatca tttgcgttaa
    10141 atatgtcaca tctgattcac cgttatagtg ttgtaaatta ctatttgtta aaaaagatat
    10201 tgctgtatta aacgctaaat ctatcgattg gtttaaatta tgatttggat ttaaaaaaag
    10261 ccattgctga actattagca atacaaatgt tataaacccc ataaatccat taaatgccag
    10321 aaaatgtttg acatatgttt tagctgacat gtgttctaaa tctgtgccga taattttaaa
    10381 acacatattt tcaaatctag taaatattaa atctactctt gacgattgca ccaatgctac
    10441 gcgatataga tatccactaa aaacatacgt aatcataacc atcattgtta gaaacaaaat
    10501 tatttccatg ataaccctca cttaatatat ttctaaaatt tttcactacg aattaaggca
    10561 taaaataaat acaaaactaa tgcaataact accagtaata aaacgatgag cattgccata
    10621 acctccttac aacacaacaa catcgtaaca acttgtttat gagagaaata ttaattttca
    10681 aacttagtta ttaagaaatc attaagatgt gtatgcagaa ataaatttta tagcatttaa
    10741 ttgtgaagaa tattatgata ttgctatcga ggtgaaggtt atgtcaaaca ctgaatcgct
    10801 aaacatagga aaaaagcgtg gatctttaac aatttacatc ggttacagtc caggtgttgg
    10861 taaaacattt gagatgcttt caaatgccat tgaactattt caaagtaacg ttgatattaa
    10921 aataggatat attgaaccgc atcagcgtga tgaaacaaat gcattagctg aacaattacc
    10981 taaaatcaca accaatttta ctaaacatgg tagtcatcat tttcaatatt tagatgtcga
    11041 ccgcataatc gaagaatcgc cgacaatagt acttatcgat gagttagcac atacgaatat
    11101 ttctagagat cgtcatgaga aacgatatat ggatattgaa gaaattttaa atcatggtat
    11161 cgatgttcat accactttga acattcaaca tattgaaagt ttaagtagtc aaattgaact
    11221 gatgaccggt gtacatgtta aagaacgtgt acccgactat ttcataatga gcgccgatgt
    11281 attagaagtc gtagatatct cacctgaaca attaattaaa cgcttaaaag ctggcaaggt
    11341 atataaaaag gataggctag atgtagcatt tagtaatttc tttacgtatg cccacctaag
    11401 cgaactgcgt acattgacgt taagaacagt tgccgacttg atgagtgata aagaaaaagt
    11461 ccgacacaac cataaaacgt cactcaaacc tcatattgct gtggcaatta gtgggagcat
    11521 ttataatgaa gcagtaatta aagaggcatt ccatattgct caaaaagaac atgcgaagtt
    11581 cactgctatt tatatagatg tattcgaaaa aaacaggcaa tataaagata gtcaaaagca
    11641 agtgcatcaa catctcatgc ttgcaaaatc attaggagca aaagtaaaag tagtttatag
    11701 ccaaaccgtt gcattaggat tagacgaatg gtgtaaaaat caagatgtaa ccaaattaat
    11761 tatcggacaa catattagaa ataagtggcg agactttttc aatacacctt taattgacca
    11821 tttaatgtcc tttgaacata gctataaaat cgaaatcgtt ccaatcaaac aaatacctgt
    11881 tgaattgaaa atgaacaaat caccctatcg tcctaaaggc aaacgtttcg ccatagatat
    11941 gttaaaaatg attttgattc aaataatttg tgtaatgatg ggactgtgga tttatcaact
    12001 tgataagcat gagtctagta cgattatttt aatgattttt ctcatcggca tcattttatt
    12061 atccatttgg acgcggtcct tcatcattgg ctttttagca gcaattatta acgtatttgt
    12121 ttttaattat ttttttacgg aacctagata tacatttgaa gtatatcgct ttgactatcc
    12181 tattacattt atcgttagca ttttaacaag tattttaacg agtgctttat taaaacaaat
    12241 taaattccaa tactccatta ctaaaaagca actttatcgt acagatttat tatttcaatt
    12301 taatgattcg attaaacaaa cttatacagt tgaaaactta ctaataaatg ctggatacca
    12361 aattaatcaa ttattgcaac aatcaattac tatatacgtt atcaatcaat cgaaagtaat
    12421 taaaacaata ccattgcaaa accatatcga taatacgact caacagcatg aacaagcatt
    12481 aagctgggta attaaaaatg aaagacaagc tggtgcaaca acagatacat ttccaggcat
    12541 taataaatgg ttgataccaa ttggcacatc tccgattaaa ggcattctag ccattgatta
    12601 ccaaagttct caagtgatta atccatatga tgcatcaata ttagaatcaa tgttaaatga
    12661 actttctctc gccgttgaaa atgtgacgtt acttaaacaa acgagagaat ccatgttaca
    12721 ggctgaacgt caactgacac attcaaattt tttaagatca atttcgcatg acatacgtac
    12781 accgttaaca acaattatgg gaaatttaga tattttagta tcacatagta aagatatgtc
    12841 tatcatagaa aaggaacaat tacttgtaca tagttttcaa gaaagtcagt atttatatct
    12901 attagtgaca aatattttat cattaacaaa attacagtca tcaaatgttc agataaaatt
    12961 gcaaccttat ctcgttagtg aattagtaga agaaatcgat atgattttag aacgtcgcca
    13021 tttaaaaaag cgtataactg tatcatcctc agtaaattta cagtttatac acattgacag
    13081 taagttgatt ttacaagctt tattcaattt gattgaaaat gcagttaaac acacttctac
    13141 tgacaccaaa atcaatttat ctattcgtta tgctagctat gaacaaatcg aatttgccgt
    13201 tatagacgaa ggacctggaa ttagtttaga agaacaacaa aagatattcg agccttttta
    13261 cacaggatca aataaatatt ttaaagataa ccaaaaagaa agcatgggac taggcttata
    13321 tttagtacag actatcctac acaaacatca atcaaactta caatataaac cgaaccaacc
    13381 acacggcagt atattctatt tcaatatata tacagacttt aacgaaggag acgtataatg
    13441 caatctaaaa tattgataat tgaagatgat cacgcaatca cacatttact tgatgttgca
    13501 ttaactttag attattacaa tgtaactaca gccgacaatg ccacacaagc acactttaaa
    13561 attcaaattg ataaaccaga tgtcatttta ttagatttag gtttaccaga taaagatgga
    13621 ttatgtttga tttcagaaat caggcaacat actgacattc ctatcattgt aataagtgca
    13681 agacaagaag aacaaacaat tattcaagct ttagataacg gtgcgaatga ctatatgact
    13741 aaacctttta atgttgatga gcttcgggca cgaatacgag tcatcgagag aatcgctaag
    13801 tcacatcaag aaactaacat tgtatttact aacggtttgc taagcatcga ctttggctcc
    13861 aaatctgttg ttattaataa tcaggaggta catttgacgc caaatgaatt tagcttgtta
    13921 gaactattgt caaatcataa aggaaaagta ctcacttatg agatgatatt aaaacgaata
    13981 tatggctatg ttaataagac tgaaatgcct agtttaagag tgcatatgac atcattaaga
    14041 caaaaacttt cacaatgtca tgaggacgca aaagatatta ttaaaacaca tccacgcatt
    14101 ggttatcaaa tgttgcagtg gaaagagaaa taattaaata aaaaagatcg ctgccattaa
    14161 acgaatgatt ggcagtgatc ttttttatat tcaacttatt atttatcgcg cactagcgtc
    14221 agcaaaaagc cctattgttt tttataagaa catgatactg aaatttcata aagtttattt
    14281 atgttcgatt attcttaatt taaaaatagt tctttaaatt atatactcac cacatttggt
    14341 ggagaaccat atttacacag aatgatataa ccgcatacag atctcaatcc aattaaatat
    14401 ttaaccttta aaacgttagt actttgatat ctacagagaa tcaattattg aaaacaaaaa
    14461 agagctcaag acattacatc tttaagctct taatctataa attatttttg atggtcagca
    14521 aatgtgcgac ctttcatagg tctgcttcca ccgctgctct tctctttacg gtcgaatttt
    14581 tttgtacttt tcttcttact tgagtatcct tttgaacgtt tactcttact gtcaaattta
    14641 ggattaccac gcttactatt tctgttacga gaaccacttg gtttaccgtt acggcctttg
    14701 cgagataatg gtttttcaaa agttaattga acttcaactt catcgtttgc ttctactaac
    14761 tcttgtaaaa gtgcagcaac taaatcaaca tcgttatatt catttaacaa ctctgtagaa
    14821 atgcgtttca agcgtgattc tgactcttta gacatccagt tttcaacttt ttctttgatg
    14881 tcatcttcac gtgcttgaag tacttcttta cgatgtggtg gacgaagtgc actcattttt
    14941 ctaccgtttg catcttcaat ttgtctgata taatccattt cgattggatt aacaaacgtt
    15001 acagcgatac cttctttacc agcacgaccc gtacgaccaa tacggtgtgt atagctttca
    15061 gtatcttgag gtatatcaaa gttataaaca tgactcacac cagaaatatc tagtcctctt
    15121 gctgctacat cagtagcgac taaaatatta atttggtcat ttttaaattt ctttaatact
    15181 tctaaacgtt tcgcttgtgt aatatcacca tgtaaacctt cagctttata tcctttagaa
    15241 atcaaagcac ttgttaattc atcaacacga cgttttgtac gtccgaatac gattgctaat
    15301 tcaggttgat gaacatctag gaaatttgta aatgtatcaa atttctctaa ttctttaaca
    15361 attgtataga attcttcgat ttgtggatca gacatttcat tattcattgt cttaatgatt
    15421 tttggtgatt tcataaattg ttgtactaaa gcttggattg ctttaggcat tgtagctgag
    15481 aacaacattg tttgacgttg tactgctgga attttatcca taataaatct catatcatcg
    15541 atgaatccca tattcatcat ttcatcagct tcatctaaaa tcaaagtatg aattccgtcc
    15601 gtttttaatg tgcgacgatt taaatggtcg ataacacgcc caggtgttcc gactacgatt
    15661 tgtgggcctt ttttcaaggc tttaatttgg cgttcgatag gcataccacc gaatacagta
    15721 acaacttgga caccttgtcc acggctaaat tctcttaatt gttcagctac ctgcattgcc
    15781 aattctcttg taggtgctaa aatcaacgat tgaacccctt gtttccctac tactttctca
    15841 attaaaggaa taccgaatgc tcctgtttta cctgtaccgg tttgagcttg cccaaggata
    15901 tcaattcctt gtaacgcata agggatactg tctttttgga taggtgtcgg ctctttaaat
    15961 cccattgatt caagtgactg aaccgtatta tccgaaatcc ctagttcttt aaaattttgc
    16021 aaaataattc tcctttttac ctgtttattt ctcaatatac ttttgcatat aagttcatta
    16081 atatttcatc aacttctata tagtaccacc aaaaaaattt aatcgcaata aagcaatttg
    16141 cgaatattta ttagaatcac attaattgat tgtcatattt ttgtcaaaaa gctagcatta
    16201 gataagatgt aagcgttacg catcattcga ataatcatta taaaaaattt tttataaaat
    16261 acgtatatcg ttaacattcc acaacaaatt ttactgaaac acatgtgaaa ttagacatta
    16321 taatactaca tctttttgta aaacattata taaacattca aactacttcc tatataaagt
    16381 gtgtacatag aattaacctt gatttagttt caaatttata gatttaacat aaaaagaaaa
    16441 aggcaatttt gtatttacac acaaaaccac ctcttctttg taatctttta gtattaattc
    16501 aaatcatttt tataagtaaa aggtccctcg actaatctct atgaaattaa agcatttacc
    16561 acttcttcta atttcatacc acgtgatcct ttaactaata cacggtcatg cgcttttaaa
    16621 tcgtttatta aaacttctat catatcgtct ttagaattga agtgttgtgc tttttcgaca
    16681 tgttgctggc ccgaatcata aatatacttc gcttcattac caaacgtata caacacatct
    16741 atatgctttt cttctaaata attacctaca ccgatatgca tttctttgct attttcacct
    16801 aattctaaaa catctcctaa aattagaatg cgacgccctg tcaaagtact cagtgtatca
    16861 atagctgctc tcatacttgt aggacttgca ttataggcat catttatcac agtaatatca
    16921 ttttctaatg tatgttgttc catacgcata ccagttaagc tgacattttt taaattttga
    16981 tagattgtgt tatatatcaa acctaattca tgaccaaccg caatggcaat cgtcgcattt
    17041 ttcatattat gctttcctaa tattggcaga tcgtaatgtt ctttattatt aatcgtaaat
    17101 gaaatacctg tagtatctct atcatcaaca gaacaaacta atgcattatc agtagcaaca
    17161 ccaatactaa tacattttgc attttcaact tctttaacat gtggtttcaa taatggttca
    17221 tcgccatcat atataaacgt accattatct tttagaccta ttgtaatttc agatttagct
    17281 ttagcaatcc cctcgcgcga acctaaatct tgcatatgtg actcaccaat attagttata
    17341 actgcaatat ctggttgagc gaggtttgac agaaattcaa tttcatggaa acctgacatc
    17401 cccatctcca atattgatat ttcagtatca ttatctaatt ccaaaatagt taaaggtaaa
    17461 ccaatttcat tattgtaatt accttgcgtt ttcttaactt taaattcggt atgcaataca
    17521 ctttcaatca tatctttagt cgttgtttta ccattagacc ctgtgacggc aattacttta
    17581 gggtttacat gtctcaagta agcttgtgcc aattgttgta atgccgttaa tgtgtcttca
    17641 acccatataa taggcccgct tacattttca tctataggtg tccctctttg ataaaaagca
    17701 gccccagcac catcttgtaa tgctttagag acaaagcgat gaccgtcaac attttcacct
    17761 ttaaatggta taaataacat atttttagaa attgctcgtg aatcaattgt gactccattt
    17821 atctcttgat ttaaaaattg atcttcaatt tcacaaggaa tccatgattg aatttgcttt
    17881 aatgtaacat taatcataat aacaacctca gttagtcaat tttgtattta tttttctgtt
    17941 tatcctggtg acgttcttta gcaagctcga taagttttgt aatcaattct ggataagata
    18001 agcccatatt ttcccataac tttggataca tactgaaagc cgtaaatcca ggcattgcat
    18061 ttgtttcatt aatatatatt tggttgtctt ctgttacaaa gaaatcagca cggactaaac
    18121 cagaacaatc tgtcgctttg aatgcctcta atgccatatt tctaagcgtt aattgaacat
    18181 cttcgtctaa gtcagctgga atttgtaatt gaaccttacc atctttatat tttgatttgt
    18241 aatcgtaaaa cgcgacatct tttacgactt cacctggcca tgtcgcttca ggatagtcat
    18301 ttcctaaaac tgctacttca atttcacgtg cgttaacgcc ttgttctata acaagcttac
    18361 ggtcaaattg gaatgcttct ttaatacctt ctttaagttc cgcttcatta ttacatttac
    18421 tgatacctac acttgaccct aagttagcag gtttaacaaa gactgggtaa tttaatttat
    18481 catttactaa ttttaaaatg ttatgttcat atttttcata ttcagaacgt aagaaactaa
    18541 tataaggtaa ctgtggtaac cctcgatgtt caaataattg tttcattaca agtttgtcca
    18601 tagaacttgc agctgacaat acaccatttc ctacatatgg tacatccaaa acttcaaaaa
    18661 gcccttgaat cgtgccatct tcaccattag gaccatgtaa taatgggaat actgcatcgt
    18721 atggttgtcc tgaactactt tctttcaata gctgtgaaat ctcaagcgcc tctccatttt
    18781 ctaaatgaag ctcatcagta gatttaattt cagctgtaat attattttgc tttctccaat
    18841 caccatcatt ggtaatataa atgatatcaa catgatattt gtctttatct attgcattta
    18901 atacattttg tgctgtcaga atcgatactt cgtgttctgc acttttccct ccaaaaacga
    18961 tacaaatatt ttcttttgtc atttcgtttt cctccaatga tatatcaggg tttgctacct
    19021 taatataaat taatattgat ttagtcctat tgttaattga tttattcgca tctttgaatc
    19081 ttaatagtat cacattttaa tgctattttg cattttaaaa acgaggactt atgtgtctgt
    19141 atctatgttg attcatttat aatcagaaca atattccctt ctattttatt cctaattcaa
    19201 attgaaatct atagctgata tcactgtgat attctagcta atttttaaaa agtcatgtaa
    19261 aattgatata aacattagtg agtataaaag gagtttgcaa tgaattattc atctcgtcaa
    19321 cagccggata agcattggct tcgcaaagta gactgggtat tagtagccac tatagctgtt
    19381 ttagcaattt tcagtgttct gcttattaac tcggcaatgg gcggtggtca atacagtgct
    19441 aatttcggta tcagacaaat tttttattac attttaggtg caatttttgc aggtatcatc
    19501 atgtttattt cacctaaaaa gattaaacat tatacatatt tattgtattt cttaatctgt
    19561 ctattattaa taggcttgct cgttattcct gagtcaccta ttacacctat tatcaatggt
    19621 gccaaaagtt ggtacacgtt tggccctatc agtattcagc catctgaatt catgaaaatt
    19681 attttaattt tagcattagc gcgtgtcgtt tctagacata atcaattcac attcaataaa
    19741 tcattccaaa gtgatttgtt attatttttc aaaattattg gtgtctcgtt agtaccaagt
    19801 attttaatat tactgcaaaa tgacctagga actacattag tattagctgc tattattgca
    19861 ggtgtgatgt tagtaagtgg tataacatgg cgtatcttag cacctatctt tattacaggt
    19921 attgttggtg caatgacagt cattttaggt attctatatg cacccgcatt aattgaaaat
    19981 ttattaggtg tccaactgta tcaaatggga cgaatcaatt catggcttga cccctataca
    20041 tatagtagtg gtgatggcta tcatttaact gaatcactta aagctatcgg ttctggacag
    20101 ttactaggta aaggatacaa tcacggtgaa gtttatatac ctgaaaatca tactgacttt
    20161 atcttttcag tgattggaga ggaacttggc tttatcggtt ctgtcatatt gatcttaata
    20221 tttttatttt taatcttcca tctaataaga ttagctgcga aaattgaaga tcaatttaac
    20281 aaaatcttta tcgttggttt cgtcacttta cttgtgttcc atattttaca aaatattggt
    20341 atgacaattc agttgttacc aatcactggt attccattac catttattag ttatggtggt
    20401 agtgcgctat ggagtatgat gactggaata ggtatagtct tatcaattta ttatcatgaa
    20461 ccaaaacgat atgtcgattt ataccatcca aaaagtaatt aatttaaact attttgagtt
    20521 tcaaatatca taacttttca agatgacgtt atatagtcta tttacgtcgt cgatttaaaa
    20581 tgtcatatat agatattact cgataataac aatccctctt tgaagtacac attgtaaaat
    20641 gtacactcta aagagggatt ttgttatata gtacttgctt tcattttagt aaatccagtc
    20701 aaaaaagcta tcatcgaata gtccgacgat agctttaacg gtgtgtgatt cacaatcaca
    20761 gtcaattatt ttaattcgtg acttacttta ttagatgctg gcaaagtatt aataatgtct
    20821 aaacgagatt tagttttttt aatattcaca cctaatgatg acaatagttt gactacattt
    20881 tgaatctttt caggacgttt cattattatc acctcgtttg gatcgctttc aatattatct
    20941 taccctattc gacatacctt tactacatca tttacaaaat ttttaattta ttttcaaaat
    21001 taaaatctat agcgcactct acattatacc ctaaaataca attttgtatc cttcatcata
    21061 gatttctttt tcaatactat tcaaatttgc tggtgtctca tacgatacag ttacttgacc
    21121 atctattata ttaatattca cttgatgaac acctatcata tttgaaatgc gttctgtcaa
    21181 ttgacttact tgttcttctg tttcaattcc cgctgtgtaa atcgtatttt gatgtatcat
    21241 cttaaactcc tttagtcttt aatcaatttt tgaaaagtca ctaataactc ttccattgcc
    21301 tcttcctgag caccttgatt aactttattc ataatacaac ttttcatatg ttgttctaat
    21361 aactttatcg caacactgtt taacgccgaa cgtgtcgctc ttatttgcgt aaggacatca
    21421 tcacaataga catcttcttc aatcatgcga ttaatcgctc tcacttgtcc ttcaattcga
    21481 tttaaacgtg atttaagatt cgtttttatt tgttcagaat gatgtgcatt atcttgttca
    21541 gtcatagcat tcactccttc atttacattc cttactatac tatatcccat aaaacacaac
    21601 gtcaacacaa agtgtgtttt tcaaactaaa ttgtcatgat atttaaattg cttcatgata
    21661 caattaataa gatatactac tcgtttaaga ttttcgatgg gaggtttcaa atgatagagt
    21721 tattatccat tgcactcaag cattctaata ttattttaaa ttcaatattt attggtgcat
    21781 ttattttaaa cttattattc gcctttacca ttattttcat ggaaagacgt tctgccaatt
    21841 ctatctgggc ttggttacta gtcttagttt tcttgccttt attcggcttc attttatact
    21901 tactattagg acgacaaatt caacgtgacc aaattttcaa aattgataag gaagataaaa
    21961 aaggattaga gttaatcgtt gatgagcaat tagctgcttt aaaaaatgaa aacttttcaa
    22021 attccaatta tcaaattgta aaatttaaag aaatgattca aatgttgtta tataataacg
    22081 cagcattttt aacaacagac aacgatttaa aaatatacac agacggccaa gaaaaatttg
    22141 atgacctaat acaagacatc cgtaatgcta ctgattatat tcattttcag tactatatta
    22201 ttcaaaatga tgaattaggt cgtaccattt taaatgaact tggtaaaaaa gcggaacaag
    22261 gtgtagaagt taaaattctt tatgatgaca tgggttctcg tggactgcgt aaaaaaggct
    22321 tacgcccgtt tcgcaataaa ggtggacatg ctgaagcatt tttcccatca aaattacctt
    22381 taattaactt gcgtatgaac aatcgaaacc atcgaaaaat tgttgtaata gatgggcaaa
    22441 ttggatatgt tggtggtttt aatgttggtg atgagtactt aggtaaatca aaaaaattcg
    22501 gctattggcg agatacgcat ttacgaattg tcggggatgc agtgaatgca ttgcaattac
    22561 gatttattct agattggaat tcacaagcca cacgtgacca catctcctat gatgatcgtt
    22621 atttcccaga tgtaaattct ggtggaacaa ttggcgttca aatagcttct agtggtcctg
    22681 acgaagaatg ggaacagatt aaatacggct atttgaaaat gatttcatct gctaaaaaat
    22741 cgatttatat tcaatctccc tatttcatac ctgatcaagc ctttttagat tctattaaaa
    22801 ttgcggcatt aggtggtgtt gatgtcaata tcatgattcc taataaacct gaccatccgt
    22861 ttgttttttg ggctacttta aaaaatgcag catccttatt agatgccggt gttaaagtat
    22921 ttcactacga caatggcttt ttacactcaa aaacacttgt tatagatgat gaaattgcaa
    22981 gtgtgggaac agctaatatg gaccatcgca gtttcacatt gaatttcgaa gtcaacgctt
    23041 ttatttatga ccaacaaatt gccaaaaaat taaaacaagc ttttatagat gatttagcag
    23101 tatcttctga attaacaaaa gcacgttatg ctaagcgaag tctttggatt aaatttaaag
    23161 aaggtatttc acaattattg tcacctatct tataaaatag aaatatgagg agtgtaactt
    23221 taatgcaaca atcagacgtc attagtgctg ccaaaaaata tatggaatct attcatcaaa
    23281 atgattatac aggccatgat attgcgcatg tatatcgtgt cactgcttta gctaaatcaa
    23341 tcgctgaaaa tgaaggtgtt aatgatactt tagtcattga actcgcatgt ttgcttcatg
    23401 ataccgttga cgaaaaagtt gtagatgcta acaaacaata tgttgaattg aagtcatttt
    23461 tatcttcttt atcactatca accgaagatc aagagcacat tttatttatt attaataata
    23521 tgagctatcg caatggcaaa aatgatcatg tcactttatc tttagaaggt caaattgtca
    23581 gggatgcaga tcgtcttgat gctataggcg ctataggtgt tgcacgaaca tttcaatttg
    23641 caggacactt tggtgaacct atgtggacag aacatatgtc actagataag attaatgatg
    23701 atttagttga acagttgcca ccatctgcaa ttaaacattt ctttgaaaaa ttacttaagt
    23761 tagaatcttt aatgcataca gatacggcga agatgattgc taaagaacgt cacgacttta
    23821 tgatgatgta cttgaaacag ttttttacgg aatggaattg tcacgactag acattgaagt
    23881 tgtagtatga tgatgcgatg taatggcgtg ttgttgtgga agcttggtgt catgccatgt
    23941 tactttgatg tgttgttgtg ggagcttggt gacatgtcat gctactttga tgtgctggta
    24001 ccacgatgcg tcttgatgta gtgctatgat gtggcattgc ggtgttatgg tgttatagac
    24061 aggtttggcg ttgatgccat gttactttga tgtgctggta ccacgatgcg acttgatgta
    24121 gtgctatgat gtggcattgc ggtgttatgg tgttatagac cggtttgatg ttgatgccat
    24181 gttactttga tgtgctggtg ctacgatgcg acttgatgta gtgctatgat gtggcgttgc
    24241 gctgttatgg tgttatagcc aggtttggtg ttgatgtcat gccgttacga ttctatgata
    24301 tgttgttggg acgttgcaat gtgtattatg ccgttgtgac gttattattt cacactgtta
    24361 catgtataag tgaattgctg tggaaatttg cgacatatac tgctacactg atgaatcatt
    24421 gtgtcaagat gacattgcga tgaagaatga caactctgtt attaaccact ttttacatac
    24481 tgaaaactcg ttaatattat ttcaaataaa aacagcagta ggatgacttt cacatttgaa
    24541 atcatcttac tgctgtttct atttatcaca tattgtataa tgtgacacta agtttcgcta
    24601 ttgaagcgaa aaataatgtg cgccctataa agttaaaatt atcttcaact tttagggtgc
    24661 acattatttg gacttgctaa ggttatttct ttttcttttt agacacaact tgtgtgtttt
    24721 tgcctttttt attgctgccg ccgttgtgct ctctttcata cgcttcaatg aaaggttgta
    24781 cttctttttt agcgactttt tcataataaa tgttcgcaaa gtgtgtttga actacaagga
    24841 acgccgcact gactgaccag tacaaaccaa gtgctgatgc tgagcttaat gaaatccaga
    24901 taatcataat tggtgaaatg accatcatca tgtaacccat ttgacgttgt tcgtctggca
    24961 tcgttttact tgatacatat gcttggataa agtataaaac accggcaata attgtaatcc
    25021 aaatatcagg acgtcctaaa tcgaaccata agaagtgtgg atatttaaac aaaccatcta
    25081 caagttggtc tttaagtaca aagtataatc ccatgatgat tggtaattgg attagcattg
    25141 gtaaacaacc caacatactc ttaatcgggt tcatgtcata ctttttatat acttgcatta
    25201 attcttggtt tgcagccatt ttttcttctt gtgtacgcgc acgtttcact ttttcttgaa
    25261 ttttttcaac ttctggcttt gcaactttca ttttttgacg catcatatga ctatttttat
    25321 agtttgacaa catgaatggt aataaaataa tacgaattac caatacaagg ataataatag
    25381 ctaaaccata attgtcgttt aataagttat ttcccaacca atccaataca tttttcattg
    25441 gatctacgaa tgtattgtag aaaaacccac tacgtttttc aggtttagaa tagtcacaac
    25501 cagccaaaaa gaccataata cctaaaaata atggtagtaa cgcttttttc ttcatttttc
    25561 cacctctatc attatattca cataggattt attctatcac attaatgagt acgtatgaaa
    25621 caataagtgg aaaaatttaa ctaattatta aaaaaatctt tgaatcgatt aacagtcttt
    25681 tcaatatttt cacttttaga aatggctgaa atgactgaaa ttccattggc acctgcttct
    25741 acaatcggcg ccacattatt agtattgata ccgccaatag ctacaatcgg tagttgcgga
    25801 ttcatttctt taaacgttgc aatcatttct ggacctactg gtatatgcgc gtcatgcttc
    25861 gacggcgtag gatagattgg tccaacacct atataatcaa catgagttaa atcagatttt
    25921 gcatactcat ctaaatcact aatactaagt ccaataattt tatcagtgaa atattgtgct
    25981 atctctttga ctttcgcatc atcttgaccg acatgtatac catccgcgtt aatttctttt
    26041 gccaaggata catcatcatt aacgataaaa ggcacatcat attgatgaca gagatgctgt
    26101 aattctttag ctaatacaag tttatcgttt ccttttaaag ctgattcacc cttttctcga
    26161 aattggaaaa gtgtaatccc tgcctttaat gctgcttcta atacttcatg aatagttcga
    26221 tgactcggaa catcggaggt cccacaaata aaatacacat ttagatacga ttggttaaac
    26281 atattattcc acctcttgaa tacgaatccg ttgttgatag gttgtttcat ttaaatgata
    26341 taacgtatca agcaacaatg gtgaaaacgt accaggacca ccacaatttt cattttcagc
    26401 agctacctca gcagcaatat taaatacgct taccgcttca attaacgctt ctatgtctgg
    26461 ttctgtttct ctaaataaaa atccagcaat aacgcctcct aataaacaac cagctccagt
    26521 tactcgtgct aataatggag atccattagc taatacgaag gctttattat cttgaacaat
    26581 aacgtcctct ttacctgtga ttactattgc agttttatat gttgcgtaag cctttttcgc
    26641 tattgcaacc gcatcaagat tagcatcact atctgtacct ttcatagttg ctgtatcatc
    26701 aatcaacgct aatatttcag atgcattgcc tttaattact gatactttcg ctgatttcaa
    26761 taataattta caaaattgct ttcgatatgt agaagcacca acagctacag ggtcaaatac
    26821 aataggtaag cctgcctcat ttgccgtttg agcaatcgca ataatatctt gttcattttc
    26881 tgctgttaaa gtaccgatat tgattaatag cgcttgtgca actttgtaaa attcttcagc
    26941 ttcttcggga gcttcactca ttgcagggct agcaccaata cttaataaac cattcgctgt
    27001 aaaattttta actacatcgt ttgtataaca aattgttaat gggttttcaa tacgtatttt
    27061 atttagataa ttcatcgtct aatccctctt tctttaaata tgcaaaatga ttcactggac
    27121 ctctaccacg gccgatttca ggcgtatatt gtatactcat tgaaataaac tttttagcct
    27181 tgtgtacagc ctcaaataat ggtctacctt ttgcaagttc tgccgttata actgctgaaa
    27241 atgtacaccc tgttccatgc gtatgttttg ttttaaatcg ttcattttca aatgtttgaa
    27301 caccttcgtt agtaaataaa taatcttttg ctatatcagt atcatttgaa tgaccgcctt
    27361 taatgatgac acctttacta ccaatctcat taataaagat gcggccagcc tgcataattt
    27421 tttcttcact atcaatggtt agtcccgtta tttcttcagc ttctggtaaa ttcggtgtta
    27481 ctacgtcagc taatggcaat aacgtatgct gcaagttttg ctttgtgtca ttatccatta
    27541 atgaatcacc gcttttcgcc aacataacag gatcaattac atatggaata gattcatgtt
    27601 gcattaaata atgacgaatc gtttccatag tatctgctgt agcaatcatc cccgttttaa
    27661 tagcatgagg taaggtatca ttgaagacac tatcaagttg ttcatctacc cattgatgat
    27721 ttaaattatg aatatgttgt acgcccaatg tattttgagc aacaatactt gtaacgacgc
    27781 ccataccata tacaccacat gaatgaaatg attttaaatc agccattacg ccggcaccac
    27841 ctgttggatc agtacctgca atggttaatg ctattttagg tttaatcatt tacttttcct
    27901 ccaaattccc attgttctag tgtcatagcc atattgaaaa atcgacgctc atgtatgcaa
    27961 ctttctaaaa atacttgctt tacttgttcc aattccttgt cagacattga ttcggctaat
    28021 ttattcatta atgcctcaaa tacgttaatt atgtcatcca tttcagtact ataaaaatca
    28081 aaccattttg ccgtatcttt ttctctatta agcttatgat cactttgaga gcgcttagct
    28141 aattctgcat aaatatatgg acaaggtgcc attgcagcaa ttgtataaat agcattttca
    28201 cgactatgcg cttggaaata catatgtttt atgtaatggt cgccacttgg aggccaaact
    28261 tttgttttaa tgatttcttc gtatgattca ccaacaattt gcgctaaaat atcatgcgca
    28321 agtacttcac cttcaaccat aaattctatt tgctctacta aaaattttac gtcattcatg
    28381 ctattcattt ttggaattaa caaagcatat aagtttgtaa attcttttaa atacgcagca
    28441 tcagctttta agtaatggcg taatgcgtca gctcctatat ctccggataa catcttctga
    28501 ataaagtcat cctcataaat atcattaatg attggctttg cagcttggta caatttttgt
    28561 gaaaattcca ttgtaaaaaa tcctccctaa ataaaaaaac tacttccaac atgaaagtag
    28621 tttgatggca atgttgctat actagcccca tcacttcaat aactactttc ctacgttggt
    28681 actaaccaaa tcaggtcata agggtctgaa caattcatct cagccatatc attaggctcc
    28741 cctagtagtt ccttagtatt caattgcaaa ttaatcttag caaacggttt caacattttc
    28801 aattattgtt gctcagttgt attattatct ttaaataata attctataat gacatatatt
    28861 tgcgaaataa aaaaaccgga acatatcgag aattccccga tatattccaa tctaaaagtt
    28921 acttatataa ctattaatta gctatgcata aatggcttat gcagtaaccc aatgtccagc
    28981 gccaccagtg ttatataatt ttactgctgc ggcatcttga acactttcag gagcatttgc
    29041 tggtgataca cctttatatt tagcaggtgc tactgaatcc caagttgatt gtaagaattg
    29101 atacttacca gctgcacctg atgttggatt tacagcatga atattgccac ctgattcacg
    29161 ttgagcaatt tgttttagat gagcattcac atttactgat gaaccttctg atgattttga
    29221 ttcagttggt gttgcagtaa cttgtgaatt gtttgatgtt gatgcttgtg gttgttgagt
    29281 ttgagcattt tgtggtgctt caacttcttg tgattgtact tgattagctt gaacagctga
    29341 tggtgcaaca ttattagttg caggtgcttg tgcactcatg tctgctccat tagtacctgt
    29401 tgcatggtaa ttccaagcaa agtgtgtacc atctgattca aagtgataag taaacccttc
    29461 atagtcaaat gtataattat aagccccagc ttcaattggt ttttgattta atgtttgatc
    29521 atttgattgc gccatttgcg ctaaagatgc tttatttaag tccgcttcac ttgcatgggc
    29581 ttcgtgacct gcatttcctg ctacgattcc taaacctact gctaatgatg atgcgagtaa
    29641 tgttttcttc ataatcttaa aatcctccta caagtaattt gtttctctaa aagttttaca
    29701 gagactactg taacatctat aaaatccgtg caggttacaa caaaataaaa aaatagcctt
    29761 gttttttaca cagtgattac aacgaaatcc cccataattt cagatatgat tctatcgaga
    29821 attgtcatga cagtaaaaat gtttttttat ttgcaatttt ttatgtaatc gttttgcaat
    29881 ctatgtctat tttctattag aaaaactaca caaaaagacg acctacacaa cagtactagt
    29941 catgtaagtc gtttttaagt gagtatattt caagttaatt agatctataa aaaatttagg
    30001 atggattaaa tgtatattaa ggatgtttag gataattgga tttaaatttc taataagtca
    30061 tgattatcta tattttgaga gtcaatatct aaatactatc tgagagaatt tcattatttt
    30121 gggatttagg ggacataaat tttattgttt caacatataa ttcagtgaca aagtgtgttt
    30181 gtccgtcttt atcatacttt cttgatctca tttgaccagt tataccaacc aatgtacctt
    30241 gattagtata tttttctata ttagaagcta acttgccaaa tgctttacag aataagtaat
    30301 cacagacgat ttctccattt tcatctttat aatttcggtg cgttgcaaca caaaacgttg
    30361 caatttttct atcctccttt tcaaatattt gtgcgtcttt cgtcagtctc ccgacaatta
    30421 cgattttatt tagcatgtag tgtgtgattc acctcctatg cctatatctt aattactagc
    30481 ttaaaaatgg tcaaaatggc aaattttata taattaatag ctatagcatc tcgttatttg
    30541 ttatatttaa agaaattagt catgaaatga ccttaaatct tctaaataat tcaaaaatca
    30601 taaatattaa actaatggga tgtgggaata tgaaaacttt taaagcagta aggtttcaaa
    30661 ttgtgaatga acacggcaga attatagaat atgaattaga agatggcgta ataattaata
    30721 aagaggaaag tggcacgggt tggttgcttg aaattgtcat ttctaatgag cattatgaaa
    30781 catttaagga atatcaagac aatgaacagt tattggatat tcgtgtcgtc atcacgcgtc
    30841 cagccaatga tccagcttta tttgaatcta ctgtgaaaag tattaaaaat tttaaaacaa
    30901 caatgtctat cgtatttgaa tgtcatattt acactttacg tcaacagtat gctgaaagtt
    30961 tattagaaca acttattgat gatggattat ctggtgaaga attaaaaaaa tcatttaatc
    31021 gaatgatgca gtctaaacca aagttaaagg atgaaaaatt agaagagtaa aataaaaaac
    31081 attcaaagat tatgccaaca cattaaatct ttgaatgttt ttttgtttta ttttacatct
    31141 tgaattgcaa atgtaagttc acaactacaa gcaagttgac catcgacagt agctttagca
    31201 ttacctttac ctattggtcc tttaatttta gtgatttcta cttccaacgt taaagtatca
    31261 ccaggtacta cttgacgttt aaaacgacat ttatcaatac cagcaaataa agcgatttta
    31321 cctttatttt cttcactatt taaaatagct accgcacctg tttgagctaa cgcttcagta
    31381 attaatacgc ctggcattac cgcatactca ggaaaatgcc cttgaaagaa tggttcgttt
    31441 cctgatactt gtttaatagc cacacaacgt tgaccttctt catattcaac tactttatca
    31501 attaataaaa atggctgtct gtgaggtata atttgtttaa tttggttata atcaaaaatt
    31561 gtttccatgg ttgaaatcct ccattatatt aatttactga attaatcgtt aatacgttca
    31621 atgtctgcac ctaattgctt caatttaccg tgtaagtcaa catagcctct atctaggtgc
    31681 gttaattcag taacgcttgt tttaccatca gcaactaatc cagctaaaat taaggcggct
    31741 gctgctctta aatcagtcgc tttaacttgt gcaccttgca attgactttt accttcaagt
    31801 ttagcactac gaccttctac attgatatta gcattcatac gtttgaactc tgcaacatgc
    31861 ataaaacggt tttcaaaaac agtttcggtt acgactttat gaccatttgc cgttaataac
    31921 aatgccatca tttgtgattg catatcagtc gggaatccag gatgtggtag agttttgatg
    31981 tctacaggtt gtaattcccc ttcagcacgt acacgaatac catcttcttg atagtccaat
    32041 tcaacgccca tttcttctag tttatagact aaactcgcca tatgttcttt gattgcatca
    32101 cgtacaaaaa tatcaccacg cgttatagca ccagcgatta gtaatgtgcc tgcttcaatt
    32161 ctatctggaa tgatagcatg ttctacacca tgtaatgatt ctacaccatt gattgtaatt
    32221 gtgtctgtac cagcaccagt aattctacca cccatttcat taatgtagtt tgctaaatcg
    32281 acaatttcag gttctttagc tgcattttca attaaagtct tacccttagc taatgatgct
    32341 gccataataa tattttgtgt tgctcctaca cttggaaaat ctaaatgaat tgatgtacct
    32401 tttaatccat ctttagcatt agcataaata ttaccatttt caagatgaat ttctgcgcct
    32461 aaagcttcaa aacctttaat gtgttgctca atcggtctac ttccaattgc acaaccacca
    32521 ggcaatgcaa caatagcatg tcctagtctt gctaaaagag gtcccataac taaaatactt
    32581 gcacgcattt tactaacata ttcatatggt gcctcttcat ttagagtctt tgttgcatca
    32641 acgacaacag cattttcgtc ctttttgtat gtaacgtcag catttaaagt tgttaataca
    32701 ttatttattg tttctacatc acttaaagct ggaacattaa ctaatttgct cggtttatca
    32761 gaagctaata aagatgctgt caatattggt aatactgcat ttttagcacc ttctacttta
    32821 acttcacccg ttaatttatt tccacctttg attactattt tatccatcgt aaatcctcca
    32881 cttagaactc ctattacttt acatttccct attttaaaat tattttaagt cattatttaa
    32941 ttatatctag ttttgattac aaatcataga gttagactta ttttattaaa tattttacct
    33001 gcgtcgagta ttgtaacaaa tctacaataa aattgcttac tgcagtgcct aataaaatag
    33061 caacaaatat catacacact tgtaattgag tggcgtatcc ttttttaaaa aattgatcta
    33121 atctaattga ttgtaaagcc caataggcaa tacaaataca tacaacatgt aacactaaat
    33181 ggataactgc atattgtccg atataatcca ttttatcacc tcataagatt actgtattac
    33241 attgtacatg aaaaatttaa ataatgatac aaagtttaat acccgttttt agtaaagttt
    33301 aagctaactt actatataat tctttttaca taaatatcat aactataaaa tatcttggta
    33361 tgaaaaaatg tagataattt caaaaaatga aatgtcgact gtagatagtg gtgaaggaaa
    33421 ttttgatttt agcaatctat tagtgattgt aaaaaagtaa aactattctt gggagtcgga
    33481 cataaatcaa tgtctagact ctatagtgtt gtatttggca gtagttg
//
LOCUS       DALAGF010000019        31757 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.019, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000019 DALAGF010000000
VERSION     DALAGF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 31757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31757
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.019"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(74..1954)
                     /gene="vraE"
                     /locus_tag="O2692_000846"
     CDS             complement(74..1954)
                     /gene="vraE"
                     /locus_tag="O2692_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HCZ4298484.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFKSALFLKRRSILSMMKDSIKTDATTAKVTTAEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVNDQVITLENGSDSGRTNSILSANNKLTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
     gene            complement(1944..2702)
                     /gene="vraD"
                     /locus_tag="O2692_000847"
     CDS             complement(1944..2702)
                     /gene="vraD"
                     /locus_tag="O2692_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HCZ4298485.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFHKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            2985..3497
                     /locus_tag="O2692_000848"
     CDS             2985..3497
                     /locus_tag="O2692_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HCZ4298486.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIVYRRKAKALVGGNQNWILFMGIVDILFGLLVIFNVGASSAFFIYMFAFW
                     FIFSSISGLFTFSGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            complement(3631..4425)
                     /locus_tag="O2692_000849"
     CDS             complement(3631..4425)
                     /locus_tag="O2692_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HCZ4298487.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPLHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTHPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKFDVTKDNYKHFLEKYFGLNVTINRIEKQ"
     gene            complement(4668..5684)
                     /locus_tag="O2692_000850"
     CDS             complement(4668..5684)
                     /locus_tag="O2692_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HCZ4298488.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSR"
     gene            complement(5849..6424)
                     /locus_tag="O2692_000851"
     CDS             complement(5849..6424)
                     /locus_tag="O2692_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCZ4298489.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVVTAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYNQVASLNIHSSITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            6649..7557
                     /gene="rarD"
                     /locus_tag="O2692_000852"
     CDS             6649..7557
                     /gene="rarD"
                     /locus_tag="O2692_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCZ4298490.1"
                     /translation="MNSEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            complement(7970..9388)
                     /locus_tag="O2692_000853"
     CDS             complement(7970..9388)
                     /locus_tag="O2692_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCZ4298491.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVVALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(9626..10090)
                     /gene="bstA"
                     /locus_tag="O2692_000854"
     CDS             complement(9626..10090)
                     /gene="bstA"
                     /locus_tag="O2692_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HCZ4298492.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDAWKVEKVLDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIERILEGLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            10257..10595
                     /locus_tag="O2692_000855"
     CDS             10257..10595
                     /locus_tag="O2692_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCZ4298493.1"
                     /translation="MSVSDQIMKGLLDGAILGLIAQGETYGYEIMTKLKNQEFPEISE
                     GSIYPVLMRLNKKGYVDTTIKKSSNGGPRRKYYTITDSGLKELKVFKSKWQVLNKGMI
                     NLFGGENDVK"
     gene            10585..11352
                     /locus_tag="O2692_000856"
     CDS             10585..11352
                     /locus_tag="O2692_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298494.1"
                     /translation="MLSKQSQDFLEKLRVELLFRGKTEAEVEELLEELEDHLTMAEKN
                     NEDTSSIINTPIKSMADQLSPEISLTTGLYKYITLYFAFILAVIIIPRFLDLGTFDVT
                     IAFLLYIASIVILGVVIGMLILRNTLVRFGDQKITYVINVTYGIIVFLWMILGGLIIK
                     KYPIYSFFELSDKQSFIVGLVLLIALIGTCILIKQKIYALIVLVISLPSLIARIAAIF
                     SKEPDTFNLVSVSILIILNIAFIIYTFVNFRKLKKED"
     gene            complement(11471..12109)
                     /gene="pcp"
                     /locus_tag="O2692_000857"
     CDS             complement(11471..12109)
                     /gene="pcp"
                     /locus_tag="O2692_000857"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCZ4298495.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHTLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLHLALGTTE"
     gene            complement(12261..13217)
                     /locus_tag="O2692_000858"
     CDS             complement(12261..13217)
                     /locus_tag="O2692_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCZ4298496.1"
                     /translation="MNYQVLLYYKYMTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            13461..14432
                     /locus_tag="O2692_000859"
     CDS             13461..14432
                     /locus_tag="O2692_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HCZ4298497.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            complement(14581..15096)
                     /locus_tag="O2692_000860"
     CDS             complement(14581..15096)
                     /locus_tag="O2692_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HCZ4298498.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSVSESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGIIVTGTINREKYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            15476..16324
                     /locus_tag="O2692_000861"
     CDS             15476..16324
                     /locus_tag="O2692_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCZ4298499.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREVTKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            16353..16907
                     /locus_tag="O2692_000862"
     CDS             16353..16907
                     /locus_tag="O2692_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HCZ4298500.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMVYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            16960..18672
                     /locus_tag="O2692_000863"
     CDS             16960..18672
                     /locus_tag="O2692_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298501.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLKDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            18669..19502
                     /locus_tag="O2692_000864"
     CDS             18669..19502
                     /locus_tag="O2692_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCZ4298502.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISASSILLVKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELV
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     H"
     gene            19549..20046
                     /locus_tag="O2692_000865"
     CDS             19549..20046
                     /locus_tag="O2692_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4298503.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWRNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDDTMDDIINQGD
                     IVFYK"
     gene            20164..21288
                     /locus_tag="O2692_000866"
     CDS             20164..21288
                     /locus_tag="O2692_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCZ4298504.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRRYFKGNRDYEE"
     gene            21593..22411
                     /locus_tag="O2692_000867"
     CDS             21593..22411
                     /locus_tag="O2692_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HCZ4298505.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHK
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            22429..23043
                     /locus_tag="O2692_000868"
     CDS             22429..23043
                     /locus_tag="O2692_000868"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCZ4298506.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELAGVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            23036..24286
                     /locus_tag="O2692_000869"
     CDS             23036..24286
                     /locus_tag="O2692_000869"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCZ4298507.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            24302..25315
                     /locus_tag="O2692_000870"
     CDS             24302..25315
                     /locus_tag="O2692_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HCZ4298508.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLN
                     VFTLNIATYIFRHREETRQFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            25284..25862
                     /gene="hisB"
                     /locus_tag="O2692_000871"
     CDS             25284..25862
                     /gene="hisB"
                     /locus_tag="O2692_000871"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HCZ4298509.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            25859..26437
                     /gene="hisH"
                     /locus_tag="O2692_000872"
     CDS             25859..26437
                     /gene="hisH"
                     /locus_tag="O2692_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCZ4298510.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            26430..27134
                     /locus_tag="O2692_000873"
     CDS             26430..27134
                     /locus_tag="O2692_000873"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCZ4298511.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRRLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            27131..27889
                     /gene="hisF"
                     /locus_tag="O2692_000874"
     CDS             27131..27889
                     /gene="hisF"
                     /locus_tag="O2692_000874"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCZ4298512.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            27886..28518
                     /locus_tag="O2692_000875"
     CDS             27886..28518
                     /locus_tag="O2692_000875"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCZ4298513.1"
                     /translation="MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            28711..28890
                     /locus_tag="O2692_000876"
     CDS             28711..28890
                     /locus_tag="O2692_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298514.1"
                     /translation="MKSKLSNSGIDVTVKDVEKYMNRYNEVMKGKNGEKAKELCLSLL
                     PINIIVVFTFFVFIL"
     gene            29611..31653
                     /gene="lip1"
                     /locus_tag="O2692_000877"
     CDS             29611..31653
                     /gene="lip1"
                     /locus_tag="O2692_000877"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HCZ4298515.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDSNANNNLPSQSRIKEAPSLNKLDQTSQREIVNETEIEKVQPQQNNQANDKITNY
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNHDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTKATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKLTDTKQA"
BASE COUNT        11141 a         4791 c         5755 g        10070 t
ORIGIN      
        1 ttaaatcaat taatagttat actgcattgc ttaaaatcaa aatatctaat gaagcatctt
       61 ttaatcgtaa gtgttaaatg gttttcttaa tcaatttgtt tgaatgtaca aaagcaatta
      121 aagcaaaagt aatgtaaatg agggtgtata caacaattac tacaataacc ggcatgaagg
      181 aaatattacc catcaatttc ataaatgcta ttgcggcgaa tactgcatgt aatatagcaa
      241 tcaataatgg taaaccgaag ttgaaagtaa tttttaataa taatccctta agcatatctg
      301 tatgcgtaaa gcctatgcgt tttaatattc tgaagttact tagttcatcc tcagtttcat
      361 ccatttgttt aatataaata atacatccag ctgctactaa aaatgctaat cctaaaaatg
      421 atgtaacaaa tattagaata ccgttagtag catcgacctc ttttttcatt tcatcatacg
      481 tgatgacttt gtctccaaac tgttttgcaa ttgcttgagc tttttccttt tgtgatgttt
      541 gtttaatatc atatccataa aaagtatgaa cgttattttg tgttttcaac tgctgatact
      601 tttcaggact tacttcgatg acaggtgagt tgaagcttag atttaaagga taaaccttac
      661 ctttgtcttc ttgtgtaaca cggaaagttt cattcttagt accttttact actaaatctt
      721 tgtttaaatg gatattaata atgttaggca gcgattttgt atttgtaatg atggcattgt
      781 tgcctgttaa cttgttattt gcacttaaaa tagaattcgt gcgacctgaa tcactaccat
      841 tttccaaagt aataacctga tcattaacat tatctacagt aatagtttcg taagcatttt
      901 tagaaaatgt aatttgttgt tggcttagtt tagtttcaaa ttgtttagca tcttgagtag
      961 cgaccacgtt aaattcattt ggtgccatag atgtaagggt ttgatctgta tttgatttag
     1021 ataatgccgc aaaacacaat acagttactg taactgcaga aataattgca atgatagtta
     1081 aagacatggc atttttcttc attctgtaca taatagacga tgtgaataca acatcggtaa
     1141 tagatacgcg tccatttttt gattttttca atgttttaaa aataagtgac acggaacttc
     1201 tgaagaataa ataggcgcct acaaccgtta aaaataaaat gataaacggt gatgtcatag
     1261 ccatagttag tgctttgaac gtaccaaaca tttctgtcgc catataatag cctagtgcaa
     1321 tcatagcaat acctaatacg cctgaaataa cctctgcagt cgttacttta gcagttgtgg
     1381 catcagtttt aattgaatct ttcatcattg ataagatact acgtcttttt agaaataaag
     1441 cacttttaaa taaaatcagt acatacgcaa taattagcat gaaaatagtt aaaacaaggg
     1501 ccataggttc gaaatgtatc gataagttaa tcgataacga catcaattta gatactatgg
     1561 aaagcaataa ttgtgcacct gcaatgccac ataatacacc gacaacacct gtgattaaaa
     1621 atacgatcat ttgttcaagt gctaacattt tcaaaatgtt ttgtcgtgtt aaaccaatca
     1681 actgaaatag cgcaaattca cgtgtacggc gttttacgaa taaatgattg gcatacatta
     1741 aaaagatgac aataatgata aataaaaata ttgatccgac taaagcacct ttcttaatga
     1801 tggccatcga gtcgtcatta tttacacctt tagtaaactg taaggttgta aaactgaaat
     1861 ataagacgat gctaaaaaat aatgaaaata aatacattgc ataatgtttt aagttttgtc
     1921 gtaagttttt gaaaacgata tggttaaatg tcatttgaga caccacctaa tactgattga
     1981 agatgtacaa tgtcttcata aaaggcctgt ttagaacgtc cttcctgata aagttgtgta
     2041 tgaatttggc catcttttag catgatgaca cgttctgcaa aacttgctgc aaccggatca
     2101 tgtgtaacca tgacaatagt tgtatcaaac gatttattca tttcttccaa acgttgtaat
     2161 aggtcatttg cacttttcga gtcgagtgcg cctgttggct catctgcaaa tatgatttgt
     2221 ggtttgtgaa caaatgctct cgctgctgca gttctttgtt gttgaccacc agataattcg
     2281 ctagggtatt tatttcctag gtcataaata cctaatgctg tcgtgatcgc tttataattt
     2341 tcttccattg ttgccttcga cattttttga acagataaag gtaacataat gttttcttta
     2401 acggttaatg tcggcagaat actgtaatct tggaagatga aacctaatga ttctttgtgg
     2461 aatttggcaa gtgctttttg attaagttta ttaagctctt gtccgttagc aatcacgcta
     2521 ccgctagaaa tttggtcaat tgaacttagt acatttaata aggttgtctt acctgatcca
     2581 gaaggcccca taatcgcaac gaattcgcct ttttgtatgt caaagttaat atctttaagt
     2641 gcttgaaatg tgtgcttttt accgtatgtt tttgaaacat gttgcactga taatatcgtc
     2701 atagtctcac tccttttgta tttaatttca ttttaaataa tgtttggagt agtagccttt
     2761 atctaaactt acaattcaat gaatgaacct tacagagttg aaagctatcg ctacttagta
     2821 gatttttgag tgaggataca gattcatcgt acatattaga caaaagcaat ggtgctttct
     2881 aagtgatgat gtttgtgtaa attgagaaaa gggaatttaa ttattgtata ataaattttt
     2941 tgtaaaaatt aaaagagggt tttatttgaa aggaattgat tgttatggaa aaaggaaatc
     3001 aaggtattaa atggtctagt ttaataatgg gtgtattatt attaatgttg gcagtcgtta
     3061 tttttacatt tccaattgaa aatttttatg ctattacctg gttgattgga ctgtttgtat
     3121 taattaacgg tgtgattcaa atcgtttacc gtagaaaagc aaaagcttta gtaggtggta
     3181 accaaaattg gattctgttt atggggattg tagatattct atttggtcta ttagttattt
     3241 ttaatgttgg cgcaagttca gcattcttta tttatatgtt tgctttttgg tttattttta
     3301 gttctatctc tggattattt acgttttcgg gtagtggtag cttaaaacta atttcagtga
     3361 tttttaattt attaggtatt gttttcggtg tcattttatt atttaatcca ttaatgggta
     3421 tcgtctttat ttcgacgatg attgctattg catttgtatt cgtaggtgtc atttatgttg
     3481 tagatgcact tgcttaagta aaatgaagcg gttcaaaaga agggtgtgac atgaagtttg
     3541 tgtcatatcc tttttgttgt gtttatgaag cataaaaaag gggcgctacc tacaataagt
     3601 aagatacgcc catattttta tattttacta ttattgtttt tcaatacgat taatagttac
     3661 atttagtcca aaatattttt ctaaaaaatg tttatagtta tctttagtga catcaaattt
     3721 ttctgagcta ccattccttg ttaaagttaa atgattttca gacattgtag cacggccaaa
     3781 tgattgtggc attgtaatta ataaatgctg tacaaatatt gaatctggat gcgtttgatt
     3841 atattcgatg ttgctattaa aatcttctat ttgtttaggt ttgaattcag cttcatattt
     3901 tgtatgccaa tgatcatttt cgaatttttg aacatagaaa atatccttgt cttcgttgtt
     3961 aaaaatagcg cggaatgtac cactgatatc agtaattggt tgtgtatgct cagatgaagt
     4021 aataggaatg gcatgtagag gtaagtctcc aaagccaaca tcagttacat agaatacatc
     4081 atttatagaa acaacaagtg aagcatgtga accgttcaga ctacgaccgc caccgggtgt
     4141 gtgaatagta gctgacatta attcaggatt aaatcctttt tgttgtaaat aggctttgaa
     4201 aaatgtattt aattcataac aaaaaccacc acgtttatca tgaacaattt tattaaaaag
     4261 tgcatcgata tttatagata tcggcttact attttgaaca tcaatatttt caaaaggtac
     4321 agttaacata aaacgtgttg cataataatt taatgcttca atactcggtc gattataacg
     4381 agatgaatca atttgtaaat aattctctaa cttcgcaata ttcataagca tagcgcctcc
     4441 tgtattaaag attataatta aattttaaac agaaatactg aaattttaaa ttcgaaagca
     4501 ttgaattttg gataaataca ttttaaatag aaaaatacgc tctcaaaatg aagtcatctc
     4561 taaaagaaac gatttaaaga tgactactga gagcgtagca taatggaaga agtgtgcagg
     4621 gtgtctaaaa atgcaacaat acaaaggtag ttgcaagaca agttgcctta tctagaccat
     4681 ttgtgttcta tgcgaccaaa cttccaaatt aaacttgaaa taagccaagt aattaaaaat
     4741 aatgcaacta aaatatagcc taaataatca aattcgatcg aaccaatgaa tgcccaaaac
     4801 gcaccatgta aatctaactt atcagcaaga atttgtagca attcaatcat cccaatcact
     4861 aatgctgcca tgactgatat cgcagtaatc gttatattgt aatagatttt gcgaatagga
     4921 ttgaagaatg cccaattata ggcatacttc attacaacac catctaatgt atccaataaa
     4981 ctcatacctg atgcgaataa aattggtaaa gataagattc cgataaatga aatggcttgt
     5041 tgtgatgcgc ctgaagaaag agcgagtaac gcaatttcac tagctgtatc aaaaccaagt
     5101 ccaaataaaa agccaagtgg caatacgtgc caactacgcg tgattaattt gaaataaggt
     5161 cctacaaatc gagaaaccaa tcctctagat tcaagtaatg catcgacttc agcttcttca
     5221 atgtgttcac gacgtaattt agcgaacaag ttaattaaag agattaaaat aattagattc
     5281 aacacaccga taagcactaa aaagaaacct gaaactagtg taccaatcgt tccaccaata
     5341 tcttggaaat gcggtaattc atctttagcc cattttacag atacccctaa aaaaacagcc
     5401 attaaaaata cgacagatga atgtccaatt gaaaaataga aacccacacc agatggatct
     5461 ttgcgttgct gtaataattt gcgaaccgta ttatctattg cagcaatgtg atctgcatca
     5521 aatgcatgac gcaaacctaa tgtatatgca agaatcccca taccaaataa gatatgatgg
     5581 tcttttccag caatccataa aaaactaaac ccaataacgt gtaacaaaat gacaatagct
     5641 atgtatggta accagcttaa gcgctcattt ttaaaaacag tcaaaatata tccttctttc
     5701 ataacatgat atttaatcat actgtatgtt caatgggcac tctagtaata agtgttcata
     5761 taacaaaaat gttatgccaa attatttgtt atataaaaat atacatgtaa ccacaaaaga
     5821 ttttttgcga tatatataat ttgataaatt aaccaacaac aatgtaagat gtcactttgc
     5881 ttaacttggc atccttttta tgattttcaa attcaaaaaa atgagcaaaa tgaatctctt
     5941 taccagtttt taatatttca ataccatgca tggaacctaa gcacccatgt gtgatgctgg
     6001 aatggatatt gagactagca acctgattgt aatgattaga tagttcttga attaatattt
     6061 gaggtccgta tatgtcaaag cggccaggga cagaccaaat aaattctgtt gtaaccagtg
     6121 aacgtaataa ttcaatatct aatgctgctg taacaactat aaaatctatc atttgttgac
     6181 gtttaggcgc atgattgcat gacacatctc ctgttaactt aaaaggtaat gatgactgaa
     6241 cttccgtttt aaaatgtagt tggtgctgaa ataaagcttg ttccaatatt gatatagctt
     6301 taggaccaac gccatgcatc tccattagag atgacttcgt atattgtgat actgcttcta
     6361 atgtgtatat accttgtgaa tttagcgcgc gtgttgcagg ctttccaatt ttaggtaaag
     6421 tcatttgtat ccatccttta tgttgtatgg tagtctttct ggcaatatac cctgatttgt
     6481 acagaattat ttataaaatt aatattgttt agtggttggt gaataaatgt gggaaatata
     6541 aacaaaatga tatctttatc gaaatattat tagtaaaatc gttatattag cataaccaag
     6601 tgagcgtatg cagtaaatta aaaattagga aagtaaggag gtttgaaggt gaattcagag
     6661 tataaaaaag gcatattttt agcactcagt gcatacattc tgtggggaat actacctata
     6721 tattggcagt tcgttgatgc aataggcgca tttgaaattt tagcctttcg tattatattt
     6781 tcagcaatat tcatgatttt catactcgcg gttggacaaa aacaacgcaa tgcatttcaa
     6841 cgagatatga atcaattgtt aggcaagccc attcagctat tagcgattgt cgtagcaggc
     6901 tatgtcatta cattaaattg gggtacattt atttgggctg taacgaacgg tcacgtccta
     6961 caaacaagtt taggttatta tataaatcca cttgttagca ttttgctcgc acttatcttt
     7021 ttaaaagaaa gattcaataa atttgaatgg ctagccattt tattcgcatt catcggtgta
     7081 ttatatatga cgctcaagat tggagaattc ccaatcgtct ctattatatt agcgttatcc
     7141 tttggtacat acggattatt gaaaaaagta gtacatattg atgccatcag cagtattacg
     7201 attgaatgta ttgttaccgc acctgctgga ctaatatacg ttatttattt atggcagcaa
     7261 catcagatgt catttggatt gaacatgtca tcattttggt tgttattttc tggtgctatt
     7321 acggcaatac cactaatcct attctcagcc ggggcaaaac gtattccact ttcgctaata
     7381 ggatttattc aatacgttgg accaacaata atgtttgtac tcggcatatt tgttttcaaa
     7441 gagcctttta gtatagatca attaattacg tttatattta tttggacagg tattgtgtta
     7501 tatagtcttt ctcaatacat taaattgaag aaacatccgg tcgcaaaaac cctataaaat
     7561 acgaggaaag ataattatac gtgttttcaa tatcctttgt gtcatgatga ggtgagactg
     7621 aggtatgtgt gcagtaacag tgacagacgt ataaatttga aacattaaat atgaaataag
     7681 gcactttcgt tgaattcgca attaaaatga atttaaacga aggtgttttt tgtatgatga
     7741 tttatgaggg catacgtaat gttaatcaat gaagcggaat agtatagaaa tgtattttat
     7801 gtgatgttac gtaatgactt aaatctttat acaaattttc agtgaagcac gcaatgattt
     7861 aaaggataaa cgtattttaa aaagggagtc ttgatttagc aaaggttgat tttaaataca
     7921 aattaaaaag cgcccaaatc aatgagattc gagcgctaat ttaaatattt taaaatatac
     7981 caattacttt catccaaagt gatcctaaac caatccagat aataaagtag acgaaaccta
     8041 atattaagtt cattgtccac cagcgttttt gagtcacgta acctgaagag aatagaatcg
     8101 gcgctggacc actactatag tgtgttgttg aagctaatag gttaccgaag aaacctaaca
     8161 ttaatgcact gaataatggt ggtgcaccgg ctgcgatggc aacgcctagt aatgctgcat
     8221 acatcgcact gatatgtgct gtagaacttg caaataagta atgtgaatag aagtagaaca
     8281 atattaaaat gaccaggact ataggccagc ttaagccacc aagacttgta gcaatggatt
     8341 tacttaacca aggaataaat ccaagcttgt ttaattggtc ggccattaac actaatactg
     8401 agaaccatac taatgtgttc caagcacctg tttcgtttaa aatgtcttgc catgttaaga
     8461 cgcctgtcaa taataacaat gctagcgcaa taaaggccgt taaagttgca tcaatatgaa
     8521 tgaaacttcc gacaatccat agtgttaacg caacgacaaa aataccaatc ataaattttt
     8581 cagctaaagc gattttaccc atagtcgcta attcattttc agcccaactc ttagcatttg
     8641 gtgtttcttt aacagttggt ggataaattt tataaataat aaaaggtaca acaattaagg
     8701 aaacaagtcc aggaactaaa gcagctagaa accaattcat ccatgtaatg tgaacattag
     8761 atgtgctaga tgctaaattt tgtgcaaggg ggttaccggc cattgcagtt aaaaacatag
     8821 ccgcagtaat taaattacct tggaattctg tgaaaacaag aaatgcaccc attttgcgtg
     8881 ctgatccgtc tttcggtttc gaaccaaatg attcagaaag tgatttgata attgggaaca
     8941 tgattccacc agcacgcgcg gtattacttg gtgtagcagg cgctagaatt aaatctacac
     9001 cgacgataga atatgctaat cctaatgttt ttttaccaaa taatttgacg aaatgaagtg
     9061 cgatacgtct accaagacct gttttcacaa atcctctcga aatgaaaaat gccatagcaa
     9121 ttaaccaaat gctattatta ccaaaaccag cgacagccgt tttcatgtca acaatgccaa
     9181 cgagtaccat gattgtaaat ccaattatag agacggcccc aattggcatc ggttgtgtaa
     9241 tacaagcaat gattgtcgcg acgaatattg cgaacatata ccatgctgtt ggatccacag
     9301 catccggttt aaaaggtgta agtgcccaaa taaggagacc tacaacgata gggagtataa
     9361 acttacgata tttaaccgtg ttttccatgt taaaacgtcc ttctttctat gttttataca
     9421 tatttcaatt taagaataaa gctaactaca aaagatgtac agtaataatt aaatataaaa
     9481 ttaaattaac gaaatcatta atataattat ttttcgagaa tcggtgaaga actggtatag
     9541 atggtgttta ttaaattcaa aaaagtgacc ttttaaacaa ataaaaaacc cgttaacgtg
     9601 ttgttaacgg ttaataagca catatttatt ttaaattttt taatatacga gacatctctt
     9661 caattttacc agcatgaagt gggatgtgaa tggcatttaa tactaataac tcttctaagt
     9721 tattacaacc gacaattggc tgtttcaatt caattgctaa atcatcttca gttagatttc
     9781 gtgcacgttc aggtaaagtt tggagacctt ctaaaatcct ttcaatactc ggtacttcat
     9841 ccttccattc atctggagac gaaccatttc cgaataaagg tctatagata tttaaatcaa
     9901 tattctcttt accagcaaca gctaaggccg attcaaaaat tgttaataca tgaccaaatt
     9961 gccaatgaat ggtattagaa aaaggcttat gataatcatc aagtaccttt tcaactttcc
    10021 aagcatcata cacatttact aaatatccta accccatatc aatgacatca aatactgttt
    10081 ttgtagtcat tattaattcc tcccccataa ataaaatata cttccgtaag tattacccta
    10141 ttaacaaagt tgttagtcat ttaaggaatg tgtacaacaa ctaccttaca ataaaagata
    10201 gttgacgaaa agttctcaat gtactatctt attatgtaag atagtaggtg attaaaatgt
    10261 cagtgtcaga tcagattatg aaaggtctct tagatggggc gatattaggt ctcattgccc
    10321 aaggcgaaac gtatggatat gaaattatga cgaaacttaa gaatcaagag tttccagaga
    10381 taagtgaggg tagtatttat ccagttttaa tgcgtttaaa caaaaaagga tacgttgata
    10441 cgacaataaa aaaatctagt aatggcggcc cgaggcggaa atactatacg attaccgatt
    10501 caggtttaaa ggaactaaaa gtatttaaat cgaaatggca agttttgaat aaagggatga
    10561 ttaacttgtt tggaggggaa aacgatgtta agtaaacaat ctcaagattt tttagaaaaa
    10621 ttacgtgttg aattgttatt tagaggtaag actgaagcag aagtagaaga gttattggaa
    10681 gagctcgaag atcatttaac tatggctgaa aaaaataacg aagatacaag ttcaattatt
    10741 aatacaccga ttaagtcaat ggctgatcaa ttatcacctg agataagtct tactaccggt
    10801 ttgtataaat atattacttt gtacttcgct tttattttag cagtcattat aattcctagg
    10861 tttttagatt tgggtacatt tgatgtgaca attgcatttt tactttatat agctagtatc
    10921 gttattttag gtgttgtcat tggtatgctg attctacgaa atacattagt ccgatttggt
    10981 gaccaaaaaa taacgtatgt tataaatgtg acttatggaa ttatcgtatt tttatggatg
    11041 attttaggtg gcttgattat taaaaaatat cctatctata gtttctttga gttaagtgac
    11101 aaacagagtt tcatcgttgg gttagtactt ttaattgcgc tcattggaac ttgtatttta
    11161 attaaacaga aaatttatgc gctcatcgtg ttggtaattt cattgccaag tttgatagca
    11221 aggatagcag ctatctttag taaagaacca gacactttta atttagtttc agtaagtata
    11281 ctcatcattc taaatatagc atttattatt tatacatttg taaattttag gaagttaaaa
    11341 aaagaagact aaacaatgtc gtttagctaa aatacatcat tatagtaaaa ctgtatacca
    11401 atcatccaaa agcaccctaa agttagactt aaactaacaa attagggtgc ttttgtttgt
    11461 gcacttataa ctattccgtt gtgcccaggg ctaggtgtaa atcctcatca ttagaaatag
    11521 cttcaatggc tgcagtcaaa cctgcaacta tcttttctaa tggcatagat ggtgtatcgg
    11581 gtttaccaat gacctgctct ggtatgtatg gcacatgaat aaatccgaat cgtaggtgag
    11641 ggtaatgctt atcttgtaaa taacctaagt gataaagtgt gtgattacaa acaaatgtac
    11701 ctgcgctatt tgaaagtgct ccaggaagtc cttgattaat aatactttga gtcattgctt
    11761 taactggtaa atttgaaaaa taagctggcg caccgtctaa gtgaatggct tgatcaatag
    11821 gttgaaaatc atcattatct ggaatacgtg catcatcaat attaatggcg acacgttctg
    11881 gggtaatggc atttctacca ccagcttgtc ctatagctag tacaacatca taatgattag
    11941 atgccaacgt tttatttata atattatcta ctttcttaaa agaggttggt agttttaatt
    12001 tatcgattgt atgtgtgcca ataatatctt ctagttgagt cacagcttcc catgagggat
    12061 tgatattttg attgtcaaaa ggcgcaaacc ctgttactaa aatgtgcatt ttaagacacc
    12121 ccgcagtatt aaaatttaat ttagtataac acgcgcataa ttaaaaacat atcaggttat
    12181 gtatgcgcat aaaaaacagc cttgaaaaac ctttataaat cggattttca aagcgtgttt
    12241 tgaaaggtat ttaattaaaa ctaagcatgt tgatgtaaat catcaaagtt tgttaaacgt
    12301 tgttgccact cattatcact aatattattt gcttgaacat aacgattacg ctcatgttta
    12361 gcacattcat aagagcatgc acctaaatat ttagtttcgt tttcttcaga aactaatatt
    12421 tgtttattac attctgggtt agcgcaatta atataacgtt cacatggttt gccatcaaac
    12481 caatccttac caataattgt tttttcaact tggttgatat caacactgat acggtcatca
    12541 aatacgtaca ttttaccgtc ccaatattca ccttttgttt caggatcttt accatatgta
    12601 gcaataccgc catgaagttg agctacatct tcgaaacctt cttttaaaag ccatccagaa
    12661 aatttttcgc atcgaatgcc accagtacag tacgtaacca cttttttatc tgcaaataac
    12721 gctttattct ctttaatcca gtcaggcaaa tctctaaaac gtgtgatatt tggacgaatt
    12781 gcacctcgga aatgacctaa atcaaattca taatcattac gtgcatcaat aatgactgtg
    12841 tcatcatctt caagagcttt tctaaattct acaggtgata aatattggcc agttgtgtgt
    12901 cttggatcga cgtcatcttc taaatctaaa gcaacgattt cttttcgagg acgtacatgc
    12961 attttcttaa aagcatgtcc ttcagcttca tcaattttaa acaccatatc tttgaatcgt
    13021 tcatcggcat gcatatgtgc catatattgt tcggtttctt cttttgtacc agataatgtg
    13081 ccgttaatac cttctgtaga aacaagaatt ctacctttta aatggtgtgc tttacaaaag
    13141 gctaagtgat cctgagcaaa ctgttcaggg tcatcaatcg tcatatattt ataatataaa
    13201 agaacttgat agttcatagg ttaatctcct tctataaaca tgttataagc atgttaattt
    13261 ttaaaataaa gcgtcaatac gcatatttgt ctacgtccat cattttaaca gagttgtatt
    13321 gtcattcaat atttctagct tgaaatcagc cactagaccg ataaaataga tagatgaaac
    13381 cgttatcatt ttatttgata gttatcttgt ttaaaataaa attgatagtt aaaacgaatg
    13441 aaatgaaagg aatgatcatg atgtcacaac aagatttacc tacattattt tatagcggga
    13501 agtccaatag tgctgttcca attatatctg aaagtgaatt acaaacaatt acagctgaac
    13561 catggcttga aatttccaaa aaaggattgc aactagaagg attgaacttt gatcggcagg
    13621 gacaactctt tttattggat gtattcgaag gcaatatttt caaaatcaat cctgaaacga
    13681 aggaaatcaa acgacctttt gtaagtcaca aagcgaatcc tgcagcaatc aaaatacata
    13741 aagatggccg attattcgtt tgttatttag gagattttaa atctacagga ggcatttttg
    13801 cagctacaga aaatggtgac aacttacaag atattattga agatctttca acagcatatt
    13861 gtattgatga catggtattt gattctaaag gtggatttta ttttacagat tttagaggat
    13921 actctaccaa tccactagga ggcgtttatt atgtttcgcc ggactttaga acagtgacgc
    13981 ctatcattca aaatattagc gtagcaaatg gtattgcttt aagtacagat gaaaaagtac
    14041 tatgggtaac agaaacaaca gccaatcgat tacatcgcat tgcacttgaa gatgatggtg
    14101 tgacgataca accatttgga gctaccatac cgtactattt tacaggtcat gaaggaccag
    14161 actcatgttg tattgatagt gacgataatt tatacgtagc aatgtatggt caaggtcgag
    14221 tgttagtttt taataaaagg ggttatccaa taggacaaat attgatacca ggccgagatg
    14281 aagggcatat gttacgttct actcatccgc aatttatacc tggaacaaat caactcatca
    14341 tttgttccaa tgatatagaa atgggcggag gatctatgct ttatacagtt aatggctttg
    14401 caaaaggtca tcaaagtttt cagtttcaat aagacctacc tgaatgaaag gcgtctaaaa
    14461 ttagggtgaa cagttagtgc ttgttacaca tgagttaaac gaaaaatgcc aactatacct
    14521 agggatgata tagttggcat ttcgtatata tattaaaaca atgaggattg tgtaatgaga
    14581 ttattctgag attgagaatt cagctgatgc ttcgaattta acatctttgc ctagcattac
    14641 gccaccagtt tcaagtgctt ggttaaagtt aatgccatat ttttctctat tgattgtacc
    14701 agtaacaata atacctgtta cttgagaacc atccatagga ttcttactta ctccgttgaa
    14761 ttcaacatcg aatgtttctt cgttagtgat gcctttaatt gttaaatcac caacaacttt
    14821 gctttcagat actgattttg tcacaaatgt aattttatca aattcgtcag taccaaagaa
    14881 atcaccagat tttaagtggt tatcacgtgc ttcgttttta gtgttaattg agcttggaat
    14941 aattgttgca gtagctttca aagtactgaa gtcattaata tctccttcaa cagctacatc
    15001 aaattgatca aatgaacctt tcactttaga aaccattaaa tgtttaattt ggaattctaa
    15061 actactgtgt gcaccatcaa aagtaaagtt agtcataata taaaacctcc atatttttat
    15121 ctcttagata tattttttat acttgatata catacaatac cacctaaaaa acaaagatgc
    15181 aagcatttta tttcgaattc gaatattttc tgtgatgtat ttaaagaatg acctataaaa
    15241 caccacacta ttgttatacc ctagtcaatt aattcaaaca taaagaagtg attacaagtc
    15301 cgtgaaattt agttaatgag ataaatctga ctattattat catttttata gtcactacta
    15361 ttgactttgc atcaagtgac tcgtactatt ttaatataat tctgaaaatt caaaatatag
    15421 aacattaaat atgtagataa tatttttttg aagttttaaa aaaaggaggc attttatggg
    15481 aaattatttg gtattgcaat cagattttgg acttagtgat ggcgctgtaa gtgcaatgta
    15541 tggagttgca tatactgtaa gtgatgatat acgtgttgaa aacttaacgc atgatatacc
    15601 accgtatgac atttgggtag cgtcatatcg tttatatcaa actgtaaaat actggcctaa
    15661 aggcacagtt tttgtatcag tggtagaccc gggtgtaggt agtgataggc ggagcattgc
    15721 ttgcttaaca tattcaggtc attacattat tacacctgat aatggttctt taagtcatat
    15781 taaacattac gaaggtatta aaaaagttat cgaaattgat gaagtgaaaa gccgattgcc
    15841 tcattcggaa gaaagtcata cttttcatgg tagagatgtt tatgcataca atggtgcgag
    15901 attagctgca aataagatag cgtttgaacg tctcggtcag gcaattaatg ttgatagtat
    15961 tgaggcatta gaaataagag aagtgacaaa aaatgaggat tctgttacag gaagcattga
    16021 tattttagat atcagatttg ggtcattatg gacaaatatt ccattggcat tcttaaaatc
    16081 cttggaaatt gttcatggta ataatttggt ggtaacaatt tatcatcaag ataagaaagt
    16141 ctatcaaaat attattaaat ttgcgcgatc gtttgcagat gttaatgtgg gtgaacctct
    16201 tgtgtatgtt aactcgctag taaatattgg tgtagcagtc aatcaagact cattttcaga
    16261 tttatatcat attggtactg gaaatgattg gacgattcat ttatctaaag caccatcaat
    16321 ataatgaatt ttttatgtaa ggagtaatga agatgaaaaa acaagatatt tcagttaaaa
    16381 ctgttgttgc aattggtata ggagcagcgg tatttgtaat tttagggcgt tttgttgtaa
    16441 taccaacagg ttttcctaat acgaatatag aaacatcata tgcattttta gcattaatat
    16501 ctgcaatatt tggacctttt gctggtttaa tgactggatt agttggacat gctattaaag
    16561 attttacgac atacggtagt gcctggtgga gttgggttat ttgctcggga attataggct
    16621 gtttatatgg ctggattgga ctaaaattaa atctttcttc aggccgattt tctaggaaat
    16681 cgatggttta ttttaatatc ggtcaaatta ttgcgaatat tatttgctgg gcacttattg
    16741 caccaacatt agatattttg atttataacg aaccagctaa caaggtttat acacaaggtg
    16801 ttatctctgc agtattaaat attatttcag ttggcattat tgggacaata ttattaaaag
    16861 catatgcttc atctcaaatt aaaaaaggta gtttacgtaa ggaatagtta ttttgttgaa
    16921 tcagatatgt aaatgaatat agaaaggtaa tgatatatca tgactgaacc aattatctcg
    16981 ttcaaagact ttagttttca atatcatagt caagcaacac ctacattaca gaatataaat
    17041 gttgatattt atccaggaga aaaagtatta gtagttggtg cttcgggtag tggtaaatcg
    17101 acttttgcaa attgcataaa cggattaatt ccatttaaaa ctaaaggtaa cataactggg
    17161 gaactatata taaataatca agatgcaacc gttagttgtt tacatgatag atctaatgtt
    17221 gttggtacag ttttaaaaga tacagatgga cagttcatag gcttaacagc agctgaagat
    17281 atggcctttt tattagaaaa taattgtgtt gaacaagatg atatgaagaa aaatgtaagt
    17341 tattgggctg aaaaagttgg catgatagaa catttaaatc accgaccgca agatttatct
    17401 ggaggtcaaa aacaacgcgt ttcattaggt ggtatattaa tccatcgtac gcctatttta
    17461 atattggatg agccactggc caatttagat cctgcgacag gacatgaaac gctgagattg
    17521 ttaaacaata ttcatgaaga aacaaagtca acgatgatta ttgtcgaaca tcgattagaa
    17581 gaatcattag atgatacgtt tgatagagtt cttcttttta aagatggaaa aatcatcgct
    17641 aatacaacgc ctagtgattt gctaaaatca tctaagctta aagaagcagg catacgtgaa
    17701 ccattatact gtacagcgct taaatatgca gaagtagatg tcgaatccat tgataactta
    17761 gcgaatttac gtgacgtttg tatgagtgaa catgttaaat ttaaagtgaa aaaatggata
    17821 gatgaaacat ctgcaaataa tgataataag tacaaatctg aaccgctatt agaattaaat
    17881 gaggtatgtg ttcaatatag tgactatagt aatagtgtac ttaataatgt tcaattaaat
    17941 gtttatagaa gagaaatgct tagtatagtt ggtcacaatg gtgccggcaa atcaacactt
    18001 gctaaagcaa tttgtggatt tttagatata acaggtaata tccaattttg taatagggga
    18061 tttaatcaat tgtcaattag cgaacgatct gaattcgtag gttatgtgat gcaaaatcca
    18121 aatcatatga tttctgaaaa aatgatttac gatgaagtag cattagggtt aagagcacga
    18181 ggtatgaaag aatcagatat aaagatacga gtagagaatg tgctcaaaat atgcggactt
    18241 tatgcatttc ggaattggcc aattgcagct ttaagctatg gtcaaaaaaa gagggtcact
    18301 atagcatctg ttttagtctt aaatccggaa ataatcatat tggatgaacc gactgctggt
    18361 caagatttct atcattataa tgagataatg tcatttttaa ttgaactaaa cagacagggg
    18421 aagacgatta ttatgattac gcatgatatg catttattgt ctgagtatag ttcaagaaca
    18481 gttgtattat caaaaggtca agtcgttgct gataccacgc cagtattggt tttaaatgat
    18541 aaaaaaatct gtgagattgc atcattgaga caaacatcgc tatttgaaat ggccgaatat
    18601 atagggatta gcgagccaca gaaattagta caattattta ttaaccatga taggaaggtg
    18661 agacgccaat gaatcaatat aatactatag gttttcaccc gggaaatagt cgtattcatc
    18721 aattaaatgc gactgttaaa cttttattct tattagttgt ttctatttct gcaatggtga
    18781 cttatgacac aagatattta attttaatta gtgcttcatc tattttattg gtcaaatatg
    18841 ctcatattga atggaaacaa gttcgctttg ttgttaaatt cattctgttt ttcacaatat
    18901 taaatattat tgccgtgtac atatttgacc ctgaatatgg tgtgaagatt tataatcagc
    18961 gtacagagtt agtcaatggt attggtcgat ttacgctaac atcacaggaa ttattctatc
    19021 tttttaatct aatattaaaa tatattagta cagttccttt agcgttaata tttttattca
    19081 caacgaatcc gagtcatttt gctgcaagtt taaatcagct aggtgtgaat tataaaatca
    19141 gttacgcagt ctcactagca ttaaggtata ttccagatat tcaagaaaca tattttaata
    19201 tttcacaagc gcaacaagca agaggatatg ataattcgaa aaaagcaaaa tttactagcc
    19261 gtgttaaagg tatcaaacgc attgtgttac ctttaatatt ttctagtatc gaaagaattg
    19321 acactattag tactgctatg gagttaagac aattcggaca gtataaaagg agaacctggt
    19381 acgtcaaaaa acaattaaaa aaagatgatt atgttgtttt gtgtttgacg ttaatacttc
    19441 tgatgttagt agttacatta ttctttttaa ataatagtcg atatttcaac ccgtggcatt
    19501 agtattcata taaatagtct ttaaatagaa ataggaggga gacatttaat gataaatact
    19561 gaaagattaa atttaatgat tccaagttcc tcgcatttaa ttgaacttta taatatttgt
    19621 agtcatccac aagcaaatat atacactccc aaaggtttac ataattccaa attagacaca
    19681 caacggtgga ttgaaaaatg gcgaaaccat tggcaacaat atcaatttgg ttactttgta
    19741 ttggtaaaaa aaatagattg tagtgttatt ggtatttgtg gatatgaata tcgacaatta
    19801 aagcaagaaa cagtattaaa tttattttat aaattacatc caagttttga aggacaaggg
    19861 tacgcatgtg aggctattac agcaatcaca aattttgtga attatatcga tcaagaaaca
    19921 gtaaaagtta tcaggacaaa taagtgtaac caacgttcaa taaatttagc agaaaggctt
    19981 aaattcaagc gagacgatac tatggacgac attatcaatc aaggagatat tgtgttttat
    20041 aaataaaata ctatgacatt atctaaaaaa taaaattaaa atcccaacag tttcttaaaa
    20101 agttatgagc cacataataa aaatgttata attgtgcata ataaaacata gggagaatgt
    20161 ttaatgcata aaaaatattt attgctattt tttctgttat tatcagttgc attgttatat
    20221 agctgttcca agcaaaatga gaaagctcaa aataaatctg ttaatccaaa tgagtatgca
    20281 aagcataaat cgaaaatgga tgaacattgg gaaacatatc acggccaagt ttatcatgtg
    20341 ttttatcacc cattaattac agacccaaaa gttgcgttta caggagatac acaccaagct
    20401 aagggcaaca atgattggat gattactgta agtgaattta aaaaatcatt gaacgaatta
    20461 tataaaaata attacatcat cgttaatcca catgatgtat acgacttatc atctcatcat
    20521 gtaaaagcca aaaaaataaa attgccgaaa ggtaaaaagc cactaatcat ctcaattgat
    20581 gatatgaatt actatagtta catgagacac catggttatg ctgaccgttt agtactcgat
    20641 aaaaataaac atgttgtttc tgaaactaca gacaaaaaag gccatgctac ttattctgat
    20701 gacaatgata tcgtgccaat tttaaataaa tttgtaaaag agcatccaga tttttcttta
    20761 aatggtgaaa aaggtgttgt tgctttaacc ggttatgagg gcgtattagg ttatcgcacg
    20821 aatgaattaa cgagtaaaga ttatacaaag aataaaaagg cagctgagga agttgttcgc
    20881 gcaatgaaac gggatggatg gtcatttgcg agtcattcat atggtcacat taattttgaa
    20941 aaaacatctt tagaaggtat taaacgtgat actaaaagat ggaaagatga agttgaacca
    21001 attgtaggta aaacagatat gtttgtattt ccacatggcg cacaggatag acatacacaa
    21061 gcgtatgatt atttggtaga tgaagcagag tttaagttta ttgcaggtgt cggtccaaac
    21121 aattttacag atatatcagc aacgaacgtt taccaagata gggttgcgat tgatggtcta
    21181 aacttatttg aatttaaata taaactcaaa ccgtttttta atcctgaaaa tgtatatagt
    21241 aaacaagata gacgttactt taaagggaat cgggattatg aagaatagtt aaatttaata
    21301 aaaagataga aagaaaataa ctttacataa taaggcaagg acaatatgtg caagaagtac
    21361 aacttcaata taaaaatctt ggttaagcat ttttataggc acaaatgtct tgcctttttt
    21421 attttaaaaa attttaaaat caatacaccg aattttctga aaaaggttgt aatgtattgc
    21481 taaaaatgat aagttattaa tcatattaca ctttagcgtg gtagagaagt aaagcaaaca
    21541 acgaaaatac caagtgattt taaatacagt attgaaagta aagggtggtc gagtgaataa
    21601 ttcagaacaa ttaattgcgt taaaagaatc ggaaacagca tttttaaaat attttaataa
    21661 agcggattat gagttagttg attttagtgt ggttgaaaag ttggactgga aacaattgaa
    21721 ccatgaggat ttgcagcaga tgggtgagcg aaatttttgg caacatgagc atcaaattta
    21781 tgcacttcga aatgatttta cagatcaatt attgcgttat tactcaatgt atccaactgc
    21841 agcaacaaaa gtggcataca caggtttaat cattcggaat aatgaagcgg ctgtacaagt
    21901 tggtttggaa aactatgcac catcattagc gaatgtgcaa cagagcttaa agctctttat
    21961 tcagtttatt caacagcagt tgagagacaa cgtgcatttt gttgtattgg gacattatca
    22021 attgcttgat gccttactcg ataaatcgtt acaaacacca gacatactat caatgattga
    22081 ggaacgtaat ttatcgggac tggttacata tttgtcgact gaacatccaa tcgtgcaaat
    22141 cttgaaagag aatacgcaac aacaattaaa tgtattggaa cactatattc caaatgacca
    22201 tccagcactc gttgaattaa aaatatggga acgttggtta cataaacaag gttacaaaga
    22261 catccattta gatattactg cgcagccacc tagatcctat tacacaggtt tatttattca
    22321 atgtcatttt gctgaaaatg aatctcgagt tttaacaggt ggttattaca aaggaagcat
    22381 cgaagggttt ggattaggat taacacttta agtaagggag tatgcacaat gttaagaatc
    22441 gccatagcca aaggacgtct aatggatagt ttaattaact atttagatgt aattgaatat
    22501 acgacattat cagaaacatt aaaaaataga gaacgccaat tattattaag tgtagataat
    22561 attgaatgca ttttagtaaa aggaagtgac gtgccaatct atgtggaaca aggaatggca
    22621 gacataggca ttgttggtag cgacatatta gatgagcgcc aatataatgt taataatttg
    22681 ttgaatatgc cttttggagc atgtcatttt gcggttgcag cgaaacctga aacgaccaat
    22741 tatcgtaaaa tcgcaacgag ttatgttcat actgctgaaa catattttaa atcaaaaggt
    22801 attgatgtcg aattgattaa attgaatggc tctgttgaat tggccggtgt tgtagatatg
    22861 gtagacggaa ttgtcgacat cgttcaaaca ggtactacgc taaaagcgaa cggactggtt
    22921 gaaaagcaac atattagtga tatcaatgca agattaataa ctaataaagc agcttatttt
    22981 aaaaaatcac aattaataga gcaatttatt cgctctttgg aggtgtctat tgccaatgct
    23041 taatgcacaa caatttttaa atcaattttc attagaagca ccattagatg agtcattgta
    23101 tccaattatt cgcgatattt gtcaggaagt taaagttcat ggagataagg ctttgaaaat
    23161 gtataatcta acattcgatc atacgaaaac agatcattta gaaattagtc atgaacaaat
    23221 taaagcagca tttgacacat tagatgaaaa aacaaaacaa gcattacaac aaagttatga
    23281 aagaattaaa gcatatcaag aaagtattaa acagacgaat caacagttag aagaatcagt
    23341 ggagtgttat gaaatatacc atccactaga aagtgtcggt atttatgtgc ctggtggtaa
    23401 agcaagttat ccttcaacgg tattaatgac agcgacgtta gcacaagtag caggtgtgga
    23461 aaatattgtc gttgtgacac cacctcaacc taatggtgtg tctcaagaag tattagcagc
    23521 atgctatatt acgcaagttg atcaagtatt tcaagttggt ggtgctcaaa gtattgctgc
    23581 gttaacttat ggcacagaaa cgatacctaa agttgataag attgtaggtc caggcaatca
    23641 atttgttgca tatgccaaga aatatttatt tggacaagta ggtattgacc aaatagctgg
    23701 accaacagaa atagcactta ttattgatga aacagcagat ttagatgcca tcgtatatga
    23761 tgtttttgca caagcagaac atgatgaatt agcacgtaca tatgccatta gtgaagatgc
    23821 gcaagtcctt aaagatttag aatcacgtat tgctaaagca ttgcctaatg tggacagata
    23881 cgacattgtt tctaaaagta tcgctaatca acactacctt atccatgcta gtaattttga
    23941 tgaagcatgc catgtcatga atacaatcgc gcctgaacat gcgtcgattc aaacagtaaa
    24001 tcctcaacca tatattgaaa aagtgaaata tgtgggtgca ttgtttattg gacattattc
    24061 gccagaggtc ataggagatt acgttgcagg tccaagtcat gtattaccta caaatagaac
    24121 agctagattt accaatgggt tatcggtcaa tgatttctta acacggaaca cggtcatcca
    24181 tttatcaaaa gatacgtttg aacaaattgc tgattcagca caacatattg ctcatgttga
    24241 agcattatac aatcaccagc agtctatttt aatacgtcag tcttagggga gtgtaattga
    24301 aatgatttat attgataaaa atgaaagtcc agttacgccg ttggatgaaa aaacaatgac
    24361 gtctattatt agtgcaacgc catataattt atatcctgat gcagcatatg aacaattcaa
    24421 ggaagcttat gctaagtttt acggattatc gcctgaacaa attattgcag gaaatggatc
    24481 tgatgaattg attcaaaagt taatgctgat catgccagaa ggtccggcat taacgctaaa
    24541 tcctgatttt tttatgtatc aagcatatgc ggcacaagta aatcgtgaaa ttgcatttgt
    24601 agatgcagga tcagatttaa cgtttgattt ggaaaccatt ttaacgaaaa tcgatgaagt
    24661 acaaccatca ttttttatta tgagtaatcc acataaccct tcaggcaagc aatttgatac
    24721 ggcattttta acagctattg cagataagat gaaagcatta aacggatact ttgtcattga
    24781 tgaagcatat ttagattatg gtacggcata tgacgtggaa ctggcaccac acatcttaag
    24841 aatgcgtaca ttatcaaagg cgtttggaat tgccggctta agattaggtg tcttaattag
    24901 tactgctgga acgataaagc atattcaaaa aatagaacat ccatatccat taaatgtatt
    24961 tacgctaaat attgcgactt atatttttag acatagagaa gagacaagac aatttttaac
    25021 gatgcaacga cagttagctg agcagttaaa acaaatattt gatacacatg ttgcagataa
    25081 aatgtcagtg ttcccatcaa atgctaattt tgtacttact aaaggctcag cagcgcaaca
    25141 attaggacaa tacgtatatg aacaaggatt taaacctcgc ttttatgatg agccggtgat
    25201 gaagggctat gtaagatact caattgcaac agcatcacag ttaaagcaat tagaagaaat
    25261 tgttaaagaa tggagtgcaa aatatgattt atcaaaaaca acgaaacaca gctgaaacgc
    25321 aactaaatat ttcaatatct gatgatcagt caccatcgca tattaataca ggtgtgggct
    25381 ttttaaatca tatgttaacc ttgtttacat ttcatagcgg tctgtcatta aacattgagg
    25441 cacaaggtga tattgacgta gatgatcacc acgtaactga agatatcggc attgtcattg
    25501 gccaattgtt acttgaaatg attaaagata aaaagcattt cgttcgttat ggaacgatgt
    25561 acattccaat ggatgaaaca ttagcacgtg tcgttgtgga tataagtggg cgcccatacc
    25621 tatcattcaa tgcatcatta agtaaagaaa aagttggtac gtttgatacg gagttagtag
    25681 aagaattttt tagagcggtc gtaatcaatg caagattaac aacgcatatt gatttaattc
    25741 gtggaggtaa tacacaccat gaaattgaag ctatattcaa agcgttttcc cgtgcattag
    25801 gcatagcgct aactgcaact gatgatcagc gtgtgccgtc atcgaaaggt gtgattgaat
    25861 gattgtcatc gttgattatg gattagggaa tattagtaat gtaaaacgcg ctattgaaca
    25921 tttagggtat gaggtggttg tctcaaatac ctcaaaaata atcgatcaag cagaaacaat
    25981 catattgccc ggtgtcggcc attttaaaga tgcgatgtca gagataaaac gattaaatct
    26041 caatgcaata ttggctaaga atactgataa gaagatgatt ggtatttgtt taggcatgca
    26101 attaatgtat gagcatagtg atgaaggcga tgcatctgga ttagggttta tcccaggaaa
    26161 tatttcgcgt atccaaacag aatacccagt gccacactta ggctggaata atttagtgag
    26221 taagcaccct atgttaaatc aagatgttta tttcgtacat tcttatcaag cgccgatgtc
    26281 agaaaatgta attgcatatg cgcagtatgg ggctgatatt ccggcaattg ttcaatttaa
    26341 caattatatt ggtattcaat tccatcctga aaaaagcggt acatatgggt tacaaatttt
    26401 gcgtcaggca atacaagggg gatttataaa tgattgaatt atggccagcg attgatttga
    26461 ttgggtcaac aagtgtgagg ttaacagagg gtaaatatga tagtgaagaa aaaatgtcac
    26521 gctcggctga agaaagtatt gcttactata gtcaatttga atgtgtgaat cgtattcata
    26581 tcgtcgactt gataggtgct aaggcacagc atgcccgaga gtttgattat attaagtcat
    26641 tgaggagatt aacaaccaaa gatattgaag taggtggtgg cattcgtacg aagtcacaaa
    26701 tcatggacta ctttgccgca gggattaatt attgcatagt tggaacgaaa ggtattcaag
    26761 atactgattg gcttaaagag atggcacata catttccagg tcgcatttat ttatctgttg
    26821 atgcctatgg agaagatatt aaagtgaacg gatgggaaga ggacacagag ttaaatttat
    26881 ttagttttgt gagacggtta tcggatatac ctcttggcgg cattatatat actgatattg
    26941 ctaaagatgg caaaatgtcc ggacctaact ttgaattaac tggtcaatta gtaaaggcaa
    27001 cgacgattcc tgtcattgct tccggtggta ttagacatca gcaagatatt caacgattag
    27061 cgtcgctaaa tgttcacgct gctattatag gaaaggctgc acatcaagca tctttttggg
    27121 aggggctaaa atgattaaaa aacgtatcat tccatgttta gatgtcaaag atggtcgtgt
    27181 cgttaaaggg attcaattta aaggattaag ggatattggg aatcctgttg atttagcaat
    27241 gtattacaat gaagcgggtg ctgatgaatt agtattttta gacatctcta agacggaaga
    27301 gggtcatagc ttaatgctag aagtgattga acagacagcg tcacgcttgt ttatccctct
    27361 tactgtaggg ggtgggattc aaagtctcga tgatattacc caattgctaa atcatggtgc
    27421 agataaagta tcattaaatt caagtgcttt aaaaaatcca cagctcatta aacaagcgag
    27481 tgataaattc ggtagacaat gcatctgcat agcaattgat agctattatg atcctgaaag
    27541 aaaagcacat tattgttgta cgactggtgg taaaaaaatg acaaatatta aagtatatga
    27601 ctgggtacag caagtagaac agttaggtgc aggtgagctc ctcgttacaa gtatgggaca
    27661 tgatggtatg aaacaaggct ttgatattga acacctagca aatattaagt ctcttgtaaa
    27721 tattccaatc attgcttctg gtggtggtgg caatgcacaa cactttgtag aattatttga
    27781 tcagacggat gtttctgcag gtttagctgc aagtatatta catgatcgag aaacgacggt
    27841 tcaatctatt aaagaagtga tacggcaagg gggtatagca gtaagatgac caaatataaa
    27901 attgatttta gcaaaggttt agtgccagca attttacaag ataatcaaac aaaacaagta
    27961 ttgatgttgg gttatatgaa ccaagaagct tttgataaaa cgatagaaga tggtgtggta
    28021 tgtttctatt cgcggtcgaa acaacggcta tggacaaaag gtgaaacatc tggtcatacg
    28081 caacgtgtta aagatattca tgtagattgc gacaatgaca ctattttaat tgatgtcata
    28141 ccaaatggac caacatgtca tacaggcagt caaagttgtt tcaacacaga agttccattt
    28201 tcagtgcaaa cattagcgca gacagttcaa gatagtgccc aatccaataa tgaaaagtca
    28261 tatacaaaat atttattaac agaaggtata gaaaagatta caaaaaaata cggtgaagaa
    28321 gcttttgaag tcgtaattga agcaattaaa ggtgacaaaa aagcatttgt aagtgaagta
    28381 gcagatgaac tttatcattt atttgtcttg atgcatgcgc ttggcgtcga tttttcagaa
    28441 attgaggcgg aattagcgcg tagacatcat aagcgcaata actttaaagg tgaacgacaa
    28501 aatatcgaac agtggtaaag caagtatgga ctaagatata aggaaaagga tcatggctta
    28561 tacacttaca aatattgtgg aaaacgtgac attttcaagt ttaaaatacg acaccaacat
    28621 attttaacta tgaatgctgt gatggtacta aagttgcgaa ctcgttatag ataagtagtg
    28681 gataatcaca atacgaaatc aaaaataatt ataaaaagta aattgagcaa ctcaggaata
    28741 gatgtcactg ttaaagatgt cgaaaagtat atgaatcgat ataatgaagt tatgaaggga
    28801 aaaaatggcg aaaaagctaa agagttatgt ttgtcgttac tacctattaa tatcatagtt
    28861 gtctttacat tctttgtatt tatactataa atacaaatat atctagcctg aaatagaaat
    28921 gtcatagcct atttaaaaga caatctccat tagaactaag atatgcatcc cgaaagttag
    28981 actaaaaaac taactttatg ggatgtattt ttatgctaat catcataaat tcgagattaa
    29041 gttaaaggta gttcaagagt aattaaacaa taaattaaaa atagtaggat acttactttg
    29101 agggaagaaa attaactgta tatatttagt ttaggaacaa gtattacggt ttatcctgat
    29161 acaattattg tggatgggat gatattttta ggtttaaaat acgacaccag caaacataat
    29221 aactgtaata gctcataaat ctccccatat agctaatcta aaaaaataat acatcattgg
    29281 aattaagccc caagcatgta aatattaaaa atcaaaaaag atatatgtaa aaaagttaca
    29341 atttgcataa ttaaattgtg tctaattatt gactaattaa attttgccaa atataatatt
    29401 aattaataat ttgaaatgat tagcgtatac actttaaatt ctctttggag aatatatttt
    29461 ttaaatacaa atgtaaacgc tttctcgtca aattaaacaa tagaaaggat ggtcattatg
    29521 agtgcttggt taagtaaatt atttgagttt attcctcgaa taattatcaa tttgtttatc
    29581 taaaataaaa aaatagaggt gctgacaatg atgaaaagtc aaaataagta tagtattcgt
    29641 aaatttagtg taggtgcatc ttccatttta atagctacat tactattttt aagtggtgga
    29701 caagcacaag cagctgagaa gcaagtgaat atgggaaatt cacaggagga tacagttaca
    29761 gcacaatcta ttggggatca acaaactagg gaaaatgcta attatcaacg tgaaaacggt
    29821 gttgacgaac agcaacatac tgaaaattta actaagaact tgcataatga taaaacaata
    29881 tcagaagaaa atcatcgtaa aacagatgat ttgaataaag atcaactaaa ggatgataaa
    29941 aaatcatcgc ttaataataa aaatattcaa cgtgatacaa caaaaaataa caatgctaat
    30001 cctagcgatg taaatcaagg gttagaacag gctattaatg atggtaaaca aagtaaagtg
    30061 gcgtcacagc aacagtcaaa agaggcagat aatagtcaag attcaaacgc taataacaat
    30121 ctaccttcac aaagtcgaat aaaggaagca ccatcattaa ataagttaga tcaaacaagt
    30181 caacgagaaa ttgttaatga gacagaaata gagaaagtac aaccacaaca aaataatcaa
    30241 gcgaatgata aaattactaa ctacaatttt aacaatgaac aagaagtgaa acctcaaaaa
    30301 gacgaaaaaa cactatcagt ttcagattta aaaaacaatc aaaaatcacc agtagaacca
    30361 acaaaggaca atgacaagaa aaatggatta aatttattaa aaagtagtgc agtagcaacg
    30421 ttaccaaaca aagggacaaa ggaacttact gcaaaagcga aagatgatca aacgaataaa
    30481 gttgccaaac aagggcagta taaaaatcag gatcctatcg ttttagtgca tggtttcaat
    30541 gggtttacag atgatattaa tccttcagtg ttagctcatt attggggcgg taataaaatg
    30601 aacattcgcc aagatttaga agaaaatggt tacaaagctt atgaagcaag tataagtgct
    30661 tttggaagta actatgaccg cgcagttgaa ctttattatt atatcaaagg cggtcgtgta
    30721 gattatggtg cagcacatgc agcaaaatat ggacatgaac gttatggaaa aacatacgaa
    30781 ggaatttaca aagactggaa accaggacag aaggtacacc tagttggaca tagtatgggc
    30841 ggtcaaacga tacgtcaact agaagaatta ctgcgtaatg gtaatcgtga agaaatagag
    30901 tatcaaaaga aacatggtgg cgaaatttct ccactattca aaggtaatca tgacaatatg
    30961 atttcatcaa ttactacttt aggaacacca cataatggta cacacgcatc agatttagct
    31021 ggtaatgaag ctttagtgag acaaatcgta tttgatatcg gtaaaatgtt tggtaataaa
    31081 aattcaagag tagacttcgg gttggctcaa tggggtctaa aacagaagcc aaatgaatca
    31141 tatattgatt atgtcaaacg cgttaaacaa tctaatttat ggaaatcaaa agataatgga
    31201 ttttacgatc tgacgcgtga gggtgcaaca gatttaaatc gtaaaacgtc gttgaaccct
    31261 aacattgtgt ataaaacata cactggtgaa gcaacgcaca aagcattaaa tagcgataga
    31321 caaaaagcag acttaaatat gtttttccca tttgtgatta ctggtaactt aatcggtaaa
    31381 gctactgaaa aagaatggcg agaaaacgat ggtttagtat ccgttatttc ttctcaacat
    31441 ccatttaatc aagcttatac aaaagcgaca gataaaattc aaaaaggcat ttggcaagtg
    31501 acgcctacaa aacatgattg ggatcatgtt gactttgtag gacaagacag ttctgataca
    31561 gtgcgcacaa gagaagaatt acaagatttt tggcatcatt tagcagacga tttagtgaaa
    31621 actgaaaagc tgactgatac taagcaagca taatttataa agtaaaggga ggaattaata
    31681 atgactgcag acttctttca attaatcgga tcattattta gaattctaaa agaattattc
    31741 aagtaaaaca ttggcga
//
LOCUS       DALAGF010000020        30729 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.020, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000020 DALAGF010000000
VERSION     DALAGF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 30729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30729
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.020"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            197..1387
                     /locus_tag="O2692_000878"
     CDS             197..1387
                     /locus_tag="O2692_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCZ4298516.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            1496..2740
                     /locus_tag="O2692_000879"
     CDS             1496..2740
                     /locus_tag="O2692_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HCZ4298517.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVDEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(3243..4172)
                     /locus_tag="O2692_000880"
     CDS             complement(3243..4172)
                     /locus_tag="O2692_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCZ4298518.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(4414..5793)
                     /gene="argH"
                     /locus_tag="O2692_000881"
     CDS             complement(4414..5793)
                     /gene="argH"
                     /locus_tag="O2692_000881"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCZ4298519.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPSYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            complement(5783..6988)
                     /locus_tag="O2692_000882"
     CDS             complement(5783..6988)
                     /locus_tag="O2692_000882"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCZ4298520.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            7339..8670
                     /locus_tag="O2692_000883"
     CDS             7339..8670
                     /locus_tag="O2692_000883"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCZ4298521.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            8995..9570
                     /locus_tag="O2692_000884"
     CDS             8995..9570
                     /locus_tag="O2692_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCZ4298522.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            9575..10099
                     /gene="lepB"
                     /locus_tag="O2692_000885"
     CDS             9575..10099
                     /gene="lepB"
                     /locus_tag="O2692_000885"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCZ4298523.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDQDNNKHDSRQFGLIDKKDIIGNVSL
                     RYYPFSKWTVQFKS"
     gene            10115..10690
                     /gene="lepB"
                     /locus_tag="O2692_000886"
     CDS             10115..10690
                     /gene="lepB"
                     /locus_tag="O2692_000886"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCZ4298524.1"
                     /translation="MKKEILEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIVGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            10850..14326
                     /gene="addB"
                     /locus_tag="O2692_000887"
     CDS             10850..14326
                     /gene="addB"
                     /locus_tag="O2692_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HCZ4298525.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            14327..17980
                     /gene="addA"
                     /locus_tag="O2692_000888"
     CDS             14327..17980
                     /gene="addA"
                     /locus_tag="O2692_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCZ4298526.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPKNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDSVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQIEDSSRPNVNISIVYFEDVSTETILDNDEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYRYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            18146..19048
                     /locus_tag="O2692_000889"
     CDS             18146..19048
                     /locus_tag="O2692_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCZ4298527.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
     gene            19377..19766
                     /locus_tag="O2692_000890"
     CDS             19377..19766
                     /locus_tag="O2692_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HCZ4298528.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGRSPFFKPLHMILRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWI
                     TIALIIITMVLGVILPLGPISKLFGIG"
     gene            complement(19937..21253)
                     /locus_tag="O2692_000891"
     CDS             complement(19937..21253)
                     /locus_tag="O2692_000891"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HCZ4298529.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRRYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEINAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(21305..22129)
                     /locus_tag="O2692_000892"
     CDS             complement(21305..22129)
                     /locus_tag="O2692_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCZ4298530.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            22243..22551
                     /locus_tag="O2692_000893"
     CDS             22243..22551
                     /locus_tag="O2692_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCZ4298531.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
     gene            23098..24912
                     /locus_tag="O2692_000894"
     CDS             23098..24912
                     /locus_tag="O2692_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCZ4298532.1"
                     /translation="MNKTKGFTKYKKMRYIPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WYKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
     gene            25115..27724
                     /gene="clpB"
                     /locus_tag="O2692_000895"
     CDS             25115..27724
                     /gene="clpB"
                     /locus_tag="O2692_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCZ4298533.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(27783..28652)
                     /locus_tag="O2692_000896"
     CDS             complement(27783..28652)
                     /locus_tag="O2692_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ4298534.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYEKAQFLSIND
                     VHTSIKLIEMNQGISFLPLYITTNSDYNISVINTKILQAPISFTYIYSKKESPEILVF
                     IKSFKKYIANEQL"
     gene            28762..29907
                     /locus_tag="O2692_000897"
     CDS             28762..29907
                     /locus_tag="O2692_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCZ4298535.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGM
                     CTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHI
                     KEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDGTRTSREKLKEYMEIISKYQVRT
                     VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIE
                     TTFLRIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISENKPI
                     IGENIFKHESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQFPISNISSKKILHNKFK
                     SIVNTEEIDENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK"
     gene            29891..30532
                     /locus_tag="O2692_000898"
     CDS             29891..30532
                     /locus_tag="O2692_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HCZ4298536.1"
                     /translation="MEILNDSVKSFEKLYLQLQDGFPVIVPTDTNYNLCSLPNNDLCI
                     DKIFEYKKRSKDKPLSLFIDKPEDWKLYGDNQNTEIVDKLVEIFWPGPLNIILKNKTS
                     YNYMLNNSDSIAIGCVQNKTMRRFISYINSPIAITSANISGTADDILITENEAIKHMG
                     ENVRYMLRSQNKTNYKTSSTIIKVTDNKIELLREGDIKFEEIKERLGTGIIYE"
     gene            30525..>30729
                     /locus_tag="O2692_000899"
     CDS             30525..>30729
                     /locus_tag="O2692_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298537.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLIQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDS"
BASE COUNT        10798 a         4384 c         5496 g        10051 t
ORIGIN      
        1 atcaattctt ttgcttgtaa tcatttaagt aattatgtca ttataaatgt aagggttttc
       61 aaaatagaca aatttaatga aacttagtga ttatttcatc ttttttggtg gaaaagtatc
      121 tgctttgaaa actaatgtaa ttatttggat aatgtttcat gtataatagt tataaatgat
      181 gaaaagggga gatatgatga ctaaatctga aaaaattatt gagttaacaa atcattacgg
      241 agcacataat tatttaccat tgccaattgt catttcagaa gctgaagggg tatgggttaa
      301 agatcctgaa ggcaataaat atatggatat gttatctgca tattccgctg ttaaccaagg
      361 tcatagacat ccgaaaatta ttcaagcatt aaaagatcaa gctgataaag tgactttagt
      421 ttcacgtgct tttcatagtg ataacttagg tgaatggtac gaaaaaattt gtaaactggc
      481 aggtaaagat aaagctttac caatgaatac aggtgctgaa gcagtagaaa cagctttgaa
      541 agcagcacga cgctgggcat acgatgttaa aggaattgag ccaaataaag cagaaatcat
      601 tgcatttaat ggtaacttcc atggtcgaac aatggcgcca gtttcattat cttcagaagc
      661 agaataccaa cgtggttatg gtccgttatt agatggattt agaaaagttg attttggaga
      721 tgtagatgca ttgaaagctg caattaatga aaatactgca gcagttttag tagaaccaat
      781 tcaaggtgaa gcgggtataa atataccgcc agaaggatat ttgaaagcaa ttagagaatt
      841 atgtgatgaa cataatgtct tatttattgc tgacgaaatc caagcaggat taggtcgttc
      901 gggtaaatta tttgctacgg attgggataa tgtaaaacct gatgtctata ttttaggtaa
      961 agcactaggt ggtggagtct tcccaatttc tgttgtatta gcagataaag aagtattaga
     1021 tgtctttaca cctggctcac atggttcaac atttggtggt aatccacttg cttgtgctgc
     1081 atcaattgct gcattagatg ttatcgttga tgaggattta ccaggccgct ctttagaatt
     1141 aggagattat tttaaagaac aattaaagca aattgatcat ccatcaatta aagaagtccg
     1201 tggacgtggt ttgtttatag gtgtggaact taatgaaagt gctagaccat attgtgaagc
     1261 tttgaaagaa gaaggcttat tatgtaaaga aacgcatgat actgtcattc gttttgcacc
     1321 accattaatt attactaaag aagaattgga ccttgcactt gaaaaaataa gacatgtatt
     1381 tcaataatgt caaaaagttg taacaatcag tttgtaaggg ctttaaaaag gaatacgtta
     1441 tgttacaata tgtttgaaag cgaaatcatt aggtaaagaa gaggcgaaaa ggatcatgac
     1501 tgagaacaat aatttagtaa cttctactca aggaattatt aaagaagcat tgcataaatt
     1561 gggatttgac gaaggaatgt acgatttaat taaagaacct ttaagaatgt tacaagtgcg
     1621 tatccctgta cgaatggatg atggcacagt aaaaacattc acaggttacc gtgcgcaaca
     1681 taatgatgct gttggaccaa caaaaggggg cgtgcgtttc cacccagatg ttgatgaaga
     1741 agaagtaaaa gcattatcaa tgtggatgac tttgaaatgt ggcattgtaa acttaccata
     1801 cggtggtggt aagggtggta tcgtttgtga tccacgtcaa atgagcattc atgaagttga
     1861 acgtttatca cgcggatatg taagagcaat ttcacaattc gtaggtccga acaaagatat
     1921 tccagcacca gatgtattta caaactcaca aattatggct tggatgatgg atgaatatag
     1981 tgcattagat aaatttaatt caccaggttt catcacaggt aaaccaattg tattgggtgg
     2041 ttctcatgga cgcgacagat caactgcact aggtgtagtt attgcaattg aacaagctgc
     2101 aaaacgtcgt aatatgcaaa ttgaaggtgc caaggttgtt attcaaggtt tcggtaatgc
     2161 cggaagtttc ttagctaaat tcttatatga tttaggtgca aaaattgtag gtatctctga
     2221 tgcttacggt gcattacacg atccaaatgg cttagatata gattatttat tagaccgtcg
     2281 tgatagtttt ggtacggtaa caaatttatt tgaagaaaca atctcaaata aagaattgtt
     2341 tgaattagat tgtgacattt tagtaccagc ggctatttca aaccaaatta cagaagacaa
     2401 tgcacatgat attaaagcta gtatcgttgt tgaagctgct aatggaccta caacaccaga
     2461 agcaacacgt attttaactg aacgtggtat attattagtt ccagacgtat tagcaagtgc
     2521 tggtggtgta acggtttctt acttcgaatg ggtacaaaat aatcaaggtt attattggtc
     2581 tgaagaagaa gttgatgaaa agctacgtga aaaattagaa gcggcatttg atacgattta
     2641 cgaattgtct caaaaccgaa aaatagatat gagacttgca gcatatatca taggtattaa
     2701 acgtacagca gaagcagcta gatatcgtgg ttgggcataa ttaattatca tatgtgattt
     2761 aacgagcttg ggacagaaaa caaagcccta agctcgttaa ttttatttta gcagtagttg
     2821 actgtaaaac aatgcccgtg taacacgctc ttttcaaaaa tagtcggggc cccaacacag
     2881 aggctggtgg aaagtcagct tacaatagtg tgcaagttgg cggggcccca acacagaggc
     2941 tggcggaaag tcagcttaca atagtgtgca agttggggtg gagccccgac acagagaaat
     3001 tagctcctca atttctacag acaatgcaag ttggcggggc cccaacacag aagctggcga
     3061 aaagtcagct tacaatagtg tgcaagttgg ggtgggacga cgaaataaat tttgcgaaaa
     3121 tatcatttct gtcccactcc caaaaagaca gcagtaggat aatttcaatt tggaaatatc
     3181 ttactgctgt tttttatagt aatagctata tttttgtatt tatgttttct agtttaacat
     3241 tactacttaa tgacttcttt atatttatca gcgaaatttg taaagacacc atcaacgcca
     3301 tatttattta atcgtaacat atcagctttt tcattcactg tataaggatg tactataaat
     3361 cctaagtctt ttaaatgatg ggtattttgt tcagttaaat ctgtataatc aggacctaat
     3421 ccaatcgcat aagagcgtat ctcttttaag cgttggtcgt taaattgttg tagttcacct
     3481 ttatcaacta attttactaa tggcacatgc ttattttgac gatgaatttt ctttaaactt
     3541 tcgtcagaaa atgattgaat cattacatgt ccatttttta atttattgtt atttaaaagg
     3601 tgatgctttt tcaatgaagc taataattgt tcttccattc ctgggtatac atcaggtgac
     3661 tttgtttcaa tataatagtt tgcattcggg ccataacgtt ctaaaatttc atctaaagtg
     3721 ggtactttag catttttata acttgctctt gcgtattttg gatatttttt attaaaccaa
     3781 cttcctgcat ctaactgttt taattcatca agggtataat cctcaacttt accgtgtcca
     3841 tttgttgtac ggttaacagt ttcatcatgc atagcaacta aatggccatc tttggtacgt
     3901 tgtaaatcaa tttcgatata agatgctttt aactcattat gactcttatc atatgcttga
     3961 aacgtatgct cgggtgcata gccacttgcg ccacgatgtg cgatagtagt gaatcgctca
     4021 ttcgttaaat ttgtatgcca ttgaatagcc tgaggtttat ttgcaatttg atttgtttgt
     4081 tcagcgccag cagtaggtac tgataaaaat cccatagtaa aaacagcaga agcagccata
     4141 aatttagtga agcttttcga agagttagtc atagtcttta tcctttcatc tctttatttg
     4201 tagtctaaca attttaaaat actacaaaaa cctgcttgtg aaaacgcttt acacatttct
     4261 tttacaattt ataacatttt tgtaatataa atttaaaaga attatcaaac attttaggaa
     4321 tcttaaaaat gtgatgttca acaagacgaa gagagcatta aaatacccac aatgtaattt
     4381 taatagacat tgtgggtagg tagattaacg tatttattgt gatagtaatt gtttagcaac
     4441 atcaagttgt tgtttgaccg atgattgacc tgttgaaccg taactttgac gtcgttttaa
     4501 acaattttca ggctgcaaat aatcgtaaat atcggcatca atactagaat gatgttgttg
     4561 atatgttgct aaaggaacat ctaataaata atgaccttgt tgtatacatt ctaagacgat
     4621 ttttcctaca atttcatgtg cagttctaaa tggaatattt ttagttacta aataatctgc
     4681 tagttccgtt gcatttgaaa aatcttcttt aacagtttga ttgagtcgtt ctttattaat
     4741 tgtcatcgtt tgaatcatac cttcgaaaat acgtaaagaa cctttaattg tatggacagc
     4801 atcgaataaa ccttctttat cttcctgcat atctttgtta tatgctagag gtaatccttt
     4861 taaagtcata agcatgctca ttaaatgacc agtcgttcga ccaactttac ctctaattaa
     4921 ttctgccata tcaggatttt tcttttgtgg cataatagat gagccagttg aaaatgcatc
     4981 tgataatgta atgaatttag cttcgtctgt ggaccagaaa ataatttcct ctgcaaagcg
     5041 tgataagtga accatcgtta aagaaatatt atgcaatgtt tcaataatat agtctctgtc
     5101 actaacagca tctaggctat tctcatagag actgccaaag ttcaacaatg ctgttgtctc
     5161 gtgtctatcg atagggtatg tggtaccact taaggctgct gcacctaaag gattaatatc
     5221 gattcgtttt aaactatctt caaatcgttg ttggtctcgt tgtaacatcc aaaaataagt
     5281 cataatatga tgtgcaaatg aaattggctg tgcacgctgt aaatgagtat aactaggcat
     5341 aattgtatca acattattgg aagcgatgtc tacaattaca ctttgtaacg acttaattaa
     5401 tgcgatgata tcttgcactt gtttcttagt gtacaagtgc atgtctgttg caacttgatc
     5461 gtttctactg cgtccagtat gcaacttacc accagcatca ccgatacgtt taattaattc
     5521 atgttcaata tttaaatgaa tatcttctaa tgatgcacta aattgaattt gatcttgatg
     5581 ataatcatgt tgaatagatt ttagtccttg tataatttgt tcgctgtctt gttgactaat
     5641 aatgccttga ttcgcaagca tagttgcatg tgcaatgctg ccttcgatat cttgatctat
     5701 gagcgtttga tcaaaagtaa tggatgcgtt aaagtcgtca acccactctt caggttgtac
     5761 ttcaaatcta ccgccccaag ctttattgct cattgctata gcctccatgc aacatcgcat
     5821 ttacttgagt aggtaaacca tagatatcga taaagccaac agcagcgtct tgattaaatg
     5881 catcttcttt tgtataagtt gctaattttt catcatataa tgtgtaaggt gattttctac
     5941 cattcacgat ggcattacct ttgaataatt taattctgac atcaccactt acgtattgct
     6001 gagtactatc aataaataat ttcaagctat cagttaaagg tgagaaccaa agtccattgt
     6061 atagttgttc agcaaattgc ttctcaatga ttggtttaaa gtgtgcgaca tctttcgtta
     6121 acgtaatcgt ttctaatgct ttatgcgctt ttaaaataac ttctgcagca ggtgcctcat
     6181 aaatttctct tgatttgata cctacaagtc tattttctac atggtcaatt cttccgatac
     6241 catgcttacc agctaatgca ttcaacgtta aaattaaatc gtctaattca tatgttttgc
     6301 catcaatttg aactgggatg cctttatcaa acgttaaaat gatttcatca gcagtatctg
     6361 gtgtttcttc taaagcattt gttagatcga acgcatcctc tggtggcgca gcataaggat
     6421 cttctaaaat accacattca ttcgctctgc cccatagatt ttgatcgata gaataaggtg
     6481 aatcatggtt gattgataca gggatattat gtttaattgc ataatcgatt tcttcttcac
     6541 gactccatgc ccactcacgt acaggtgcga atgctttcaa tgatgggttt aatgctttaa
     6601 tggcaacttc gaaacgtact tggtcattcc ctttaccagt acaaccatgt gcaataccta
     6661 ctgaatttgt tttctcagca atctctacta attttttagc gattaatggt cttgataaag
     6721 ctgaaactaa tggatatgca ttttcataca ttaaatttcc tttgattgca taacttacat
     6781 actcatcact aaattctttt gttgcatcaa taatatgaca ttcaactgct cccatatcta
     6841 aagctttttt ataaacgatg tctaaatctt taccttcacc aacatctagg caacaagcta
     6901 caacgtcgta tcctttgtcg ataagccatt gaacggccac acttgtatct agtcctcctg
     6961 aatatgctaa aacaattttc tctttcataa aattcacctc attgtagtta tctgaatatt
     7021 tcgtatgaac acatgatagc aaacttttaa tgaaaataaa accattattt tacatattta
     7081 ttcataaaat gcgatagaga tagttagagt tgtaagtgta aatgcttatt tatcaatgtt
     7141 taaaagtatt ttaaaaaaca tgcaaattgt ataaaaaatg tttgagtgtt tgacgccagt
     7201 ttgaaatgtt tgttgaaaaa taggtgtgaa acgtcacata aatatcaatt ttattgatta
     7261 agtacaaact tgtgaattga atcgcaaacg taaacttagt aaaattaaga tattaaatga
     7321 aacaggaggc ttttatagat gactcatatt caattagatt ttagtaaaac gttagaattt
     7381 ttcggtgaac acgaattaaa acaacaacaa gaaattgtta aatcaattca caaaacaatt
     7441 catgaaggta ctggtgcagg tagtgacttc ttaggctggg ttgatttacc agttgattac
     7501 gacaaagaag aattttcaag aattgttgaa gcatcaaaac gcattaaaga aaattctgat
     7561 gttttagtag tcatcggtat tggtggttct tacttaggtg cacgtgcagc aatcgaaatg
     7621 ttaacgtcat catttagaaa cagcaatgaa taccctgaaa ttgtatttgt tggtaatcac
     7681 ttatcatcaa catatacgaa agagttagtt gattatttag cagacaaaga tttctctgta
     7741 aacgttattt ctaaatctgg tacaactaca gaaccagcag ttgcatttag attgttcaaa
     7801 caattagttg aagaaagata cggtaaagaa gaagcacaaa aacgtatatt tgcaacaacg
     7861 gataaagaaa aaggtgcttt aaaacagttg gctacaaacg aaggttatga aacgtttatc
     7921 gtacctgatg atgtaggtgg aagatattct gttttaacag cagtaggatt attaccaatt
     7981 gcaacagctg gaattaacat cgaagctatg atgattggtg ctgcaaaagc acgtgaagaa
     8041 ttatcttcag ataaattaga agaaaacatt gcataccaat atgcgacaat tcgaaacatt
     8101 ttatatgcaa aaggttatac aacagaaatg ttgattaact atgaaccatc tatgcaatac
     8161 tttaatgaat ggtggaaaca attatttggt gaatcagaag gtaaagactt caaaggtatc
     8221 tatccttcaa gtgccaacta cacaactgat ttacattctt taggtcaata tgtacaagaa
     8281 ggccgtcgct tcttattcga aacagttgta aaagtaaatc atcctaaata tgatattact
     8341 attgaaaaag atagtgatga tctagacgga ttaaattatt tagctggtaa aacaatcgac
     8401 gaagttaaca caaaagcatt cgaaggtaca ttattagcgc atactgatgg tggtgttcct
     8461 aacatggtag tgaacattcc acaattagat gaagaaactt tcggttacgt cgtatacttc
     8521 ttcgaacttg cttgtgcaat gagtggatac caattaggcg taaatccatt taaccaacct
     8581 ggtgtagaag catataaaca aaacatgttc gcattattag gtaaacctgg ttttgaagac
     8641 ttgaaaaaag aattagaaga acgtttataa aatacattac ttcaaagatt agtgaagttt
     8701 gaaaagatag aactagacgt taactattta aagcatattt tcgaggttgt cattacaaat
     8761 gtaaaaatgt aatgacaacc tcgtttttat ttatatgcaa gaactaggtt actagctaat
     8821 gtgacaagat gttaagagaa aattaaagat aaaataatat ctgccttaca ataatattgt
     8881 tatactacta gagactgatt tattagcatg attacatgtt aatgtttctt tacttagtaa
     8941 ttaactttat aatgtaagaa taattatctt caaccaaaga aagggattga tgatttgtcg
     9001 tttcatcaag tagaagaatg gtttgagata tttcgacagt ttggttattt acctggattt
     9061 atattgttat atattagagc tataattcca gtatttcctt tagcactcta tattttaatt
     9121 aacattcaag cttatggacc tattttaggt atattgatta gttggcttgg attaatttct
     9181 ggaacattta cagtctattt gatctgtaaa cgattggtga acactgagag gatgcagcga
     9241 attaaacaac gtactgctgt tcaacgcttg attagtttta ttgatcgcca aggattaatc
     9301 ccattgttta ttttactttg ttttcctttt acgccaaata cattaataaa ttttgtagcg
     9361 agtctatctc atattagacc taaatattat ttcattgttt tggcatcatc aaagttagtt
     9421 tcaacaatta ttttaggtta tttaggtaag gaaattacta caattttaac gcatccttta
     9481 agagggatat taatgttagt tgtgttggtt gtattttgga ttgttggaaa aaagttagaa
     9541 cagcatttta tgggatcgaa aaaggagtga catcgtgaaa aaagttgtaa aatatttgat
     9601 ttcattgata cttgctatta tcattgtact gttcgtacaa acttttgtaa tagttggtca
     9661 tgtcattccg aataatgata tgtcaccaac ccttaacaaa ggggatcgtg ttattgtaaa
     9721 taaaattaaa gttacattta atcaattgaa taatggtgat atcattacat ataggcgtgg
     9781 taacgagata tatactagtc gaattattgc caaacctggt caatcaatgg cgtttcgtca
     9841 gggacaatta taccgtgatg accgaccggt tgacgcatct tatgccaaga acagaaaaat
     9901 taaagatttt agtttgcgca attttaaaga attagatggt gatattattc cgccaaacaa
     9961 ttttgttgtg ctaaatgatc aagataataa caagcacgat tcaagacaat ttggtttaat
    10021 cgataaaaag gatattattg gtaatgttag tttacgatac tatccttttt caaaatggac
    10081 tgttcagttc aaatcttaaa aagaggtgtc aaaattgaaa aaagaaatat tggaatggat
    10141 tatttcaatt gcagtcgctt ttgtcatttt atttatagta ggtaaattta ttgttacgcc
    10201 atatacaatt aaaggtgaat caatggatcc aactttgaaa gatggcgagc gagtagctgt
    10261 aaacattgtt ggatataaaa caggtggttt ggaaaaaggt aatgtagttg tcttccatgc
    10321 aaacaaaaat gatgactatg ttaaacgtgt catcggtgtt cctggtgata aagtagaata
    10381 caaaaatgat acattatatg tcaatggtaa aaaacaagat gaaccatatt taaactacaa
    10441 tttaaaacat aaacaaggtg attacattac tgggactttc caagttaaag atttaccgaa
    10501 tgcgaatcct aaatcaaatg tcattccaaa aggtaaatat ttagtgcttg gagataatcg
    10561 tgaagtaagt aaagatagcc gtgcgtttgg cctcattgat gaagaccaaa ttgttggtaa
    10621 agtttcattt aggttctggc catttagtga atttaaacat aatttcaatc ctgaaaatac
    10681 taaaaattaa tatgaaacaa atacaacatc gtttgtcggt tttaatactg ataaacgatg
    10741 ttttattttg ttagtaccac aataaaagct aagttcgaaa tgaacttata ataaatcaat
    10801 cacaatcact ttgtgttaaa atatgtgtca aaggaagtga gggtttgtca tgacattaca
    10861 tgcttattta ggtagagcgg gaacaggtaa gtctacgaaa atgttgaccg aaataaaaca
    10921 aaaaatgaaa gcagatccgc ttggagatcc aatcatttta attgcgccaa ctcaaagtac
    10981 atttcaatta gaacaagcct ttgtcaatga tccggaatta aatggtagtt taagaacaga
    11041 agtgttgcat tttgaacgat taagtcatcg tattttccaa gaagttggta gttatagcga
    11101 acaaaagtta tctaaagctg caacggaaat gatgatttat aacattgttc aagaacaaca
    11161 aaagtattta aaactttatc aatcacaagc aaaatattat gggtttagtg aaaaattaac
    11221 agaacaaatt caagatttta aaaaatatgc agtaacgcct gaacatttag aacactttat
    11281 tgctgataaa aatatgcaaa ctcgaactaa aaataagtta gaggatattg ctttaatata
    11341 ccgtgagttc gaacaacgca ttcaaaacga gtttattacg ggtgaggatt cattacaata
    11401 ttttattgat tgtatgccga aatcagagtg gctaaaacgt gctgatatat atattgatgg
    11461 ttttcacaac ttttcaacga ttgagtattt aataatcaaa ggattaatta aatatgcgaa
    11521 gagtgtcaca attatattga cgacagatgg taaccacgat caatttagtt tatttagaaa
    11581 accatcggaa gtgttacgac atattgaaga aatagcaaat gaactcaata tttctattga
    11641 acgtcaatat ttcaaccaat tatatcgctt caataatcaa gatttaaagc atcttgaaca
    11701 agaatttgat gcacttcaaa tcaatcgagt ggcatgtcaa ggtcatatca atattttaga
    11761 atctgcgact atgagagagg aaataaatga aattgcgcga cgtatcatcg ttgatattcg
    11821 tgataagcaa ttacgatatc aagatattgc tattttatat cgtgatgaat cttatgctta
    11881 tttatttgat tccatattac cgctttataa tattccttat aatattgata caaagcgttc
    11941 gatgacacat catccggtca tggaaatgat tcgttcattg attgaagtta ttcaatctaa
    12001 ttggcaagtg aatccaatgc tacgcttatt gaagactgat gtgttaacgg catcatatct
    12061 aaaaagtgca tacttagttg atttacttga aaattttgta cttgaacgtg gtatatacgg
    12121 taaacgttgg ttagatgatg agctatttaa tgtcgaacat tttagcaaaa tggggcgtaa
    12181 agcgcataaa ctgaccgaag atgaacgtaa cacatttgaa caagtcgtta agttaaagaa
    12241 agatgtcatt gataaaattt tacattttga aaagcaaatg tcacaagcgg aaactgtaaa
    12301 agattttgca actgcttttt atgaaagtat ggaatatttc gaactgccaa atcaattgat
    12361 gacagagcga gatgaacttg atttaaatgg taatcatgaa aaggcggagg aaattgatca
    12421 aatatggaat ggcttaattc aaatccttga tgacttagtt ctagtatttg gagatgaacc
    12481 aatgtcgatg gaacgtttct tagaagtatt tgatattggt ttagaacaat tagaatttgt
    12541 tatgattccg caaacattgg accaagtaag tattggtacg atggatttgg ctaaagtcga
    12601 taataagcaa catgtttact tagtaggtat gaatgatgga acgatgccac aaccagtaac
    12661 tgcgtcaagc ttgattacag atgaagaaaa gaaatacttt gaacagcagg ctaatgtcga
    12721 gttaagtcca acatcagata ttttacagat ggatgaagca tttgtttgtt atgttgctat
    12781 gactagagct aagggagatg ttacattttc ttacagtcta atgggatcaa gtggtgatga
    12841 taaggagatc agcccatttt taaatcaaat tcaatcattg ttcaaccaat tggaaattac
    12901 taacattcct caataccatg aagttaaccc attgtcacta atgcaacatg ctaagcaaac
    12961 caaaattaca ttatttgaag cattgcgtgc ttggttatat gatgaaattg tggctgatag
    13021 ttggttagat gcttatcaag taattagaga tagcgatcat ttaaatcaag gtttagatta
    13081 tttaatgtca gcattaacgt ttgacaatga aactgtaaaa ttaggtgaaa cgttgtctaa
    13141 agatttatat ggtaaggaaa tcaatgccag tgtatcccgt tttgaaggtt atcaacaatg
    13201 cccatttaaa cactatgcgt cacatggtct gaaactaaat gagcgaacga agtatgaact
    13261 tcaaaacttt gatttaggtg atattttcca ttctgtttta aaatatatat ctgaacgtat
    13321 taatggcgat tttaaacaat tagacctgaa aaaaataaga caattaacga atgaagcatt
    13381 ggaagaaatt ttacctaaag ttcagtttaa tttattaaat tcttcagctt actatcgtta
    13441 tttatcaaga cgcattggcg ctattgtaga aacaacacta agcgcattaa aatatcaagg
    13501 cacgtattca aagtttatgc caaaacattt tgagacaagt tttagaagga aaccaagaac
    13561 aaatgacgaa ttaattgcac aaacattaac gacaactcaa ggtattccaa ttaatattag
    13621 agggcaaatt gaccgtatcg atacgtatac aaagaatgat acaagttttg ttaatatcat
    13681 tgactataaa tcctctgaag gtagtgcgac acttgattta acgaaagtat attatggtat
    13741 gcaaatgcaa atgatgacat acatggatat cgttttacaa aataaacaac gccttggatt
    13801 aacagatatt gtgaaaccag gtggattatt atacttccat gtacatgaac ctagaattaa
    13861 atttaaatca tggtctgata ttgatgaaga taaactagaa caagatttaa ttaaaaagtt
    13921 taagttgagt ggtttagtta atgcagacca aactgttatt gatgcattgg atattcgttt
    13981 agaacctaaa ttcacttcag atattgtacc agttggtttg aataaagatg gctctttgag
    14041 taaacgaggc agccaagtgg cagatgaagc aacgatttat aaattcatcc aacataacaa
    14101 agagaatttt atagaaacag cttcaaatat tatggatgga catactgaag ttgcaccatt
    14161 aaagtacaaa caaaaattgc catgtgcttt ttgtagttat caatcggtat gtcatgtaga
    14221 tggcatgatt gatagtaagc gatatcgaac tgtagatgaa acaataaatc caattgaagc
    14281 aattcaaaat attaacatta atgatgaatt tgggggtgag caatagatga caattccaga
    14341 gaaaccacaa ggcgtgattt ggactgacgc gcaatggcaa agtatttacg caactggaca
    14401 agatgtactt gttgcagccg cggcaggttc aggtaaaaca gctgtactag ttgagcgtat
    14461 tatccaaaag attttacgtg atggcattga tgtcgatcga cttttagtcg taacgtttac
    14521 aaacttaagc gcacgtgaaa tgaagcatcg tgtagaccaa cgtattcaag aggcatcgat
    14581 tgctgatcct gcaaatgcac acttgaaaaa ccaacgcatc aaaattcatc aagcacaaat
    14641 atctacactc catagttttt gcttgaaatt aattcaacag cattatgatg tattaaatat
    14701 tgacccgaac tttagaacaa gcagtgaagc tgaaaatatt ttattattag aacaaacgat
    14761 agatgaggtc atagaacaac attacgatat ccttgatcct gcttttattg aattaacaga
    14821 gcaattgtct tcagatagaa gtgatgatca gtttcgaatg attattaaac aattgtattt
    14881 ctttagcgtt gcaaatccaa atcctaaaaa ttggttggat caattggtga caccatacga
    14941 agaagaagca caacaagcgc aacttattca actactaaca gacttatcta aagtatttat
    15001 cacagctgct tatgatgctt taaataaggc gtatgatttg tttagtatga tggatagcgt
    15061 cgataaacat ttagctgtta tagaagatga acgacgttta atggggcgtg ttttagaagg
    15121 tggctttatt gatatacctt atttaactgg tcacgaattt ggcgcgcgtt tgcctaatgt
    15181 aacagcgaaa attaaagaag caaatgaaat gatggtcgat gccttagaag atgctaaact
    15241 tcagtataaa aaatataaat cattaattga taaagtgaag agtgattact tttcaagaga
    15301 agctgatgat ttgaaagctg atatgcaaca attggcgcca cgagtaaagt accttgcgcg
    15361 tattgtgaaa gatgttatgt cagaattcaa tcgaaaaaag cgtagcaaaa atattttgga
    15421 tttttctgat tatgaacatt ttgcattaca aattttaact aatgaggatg gttcgccttc
    15481 agaaattgcc gaatcatacc gtcaacactt ccaagaaata ttggtcgatg agtatcaaga
    15541 tacgaaccga gttcaagaga aaatactatc ttgcatcaaa acgggtgatg aacataatgg
    15601 taatttattt atggttggag atgttaagca atccatttat aaatttagac aagctgatcc
    15661 aagtttattt attgaaaagt atcaacgctt tactatagat ggagatggca ctggacgtcg
    15721 aattgatttg tcgcaaaact tccgttctcg aaaagaagta ctgtcaacga ctaactatat
    15781 attcaaacat atgatggatg aacaagtcgg tgaagtaaaa tatgatgaag cggcacagtt
    15841 gtattatggt gcaccatatg atgaatcgga ccatccagta aacttaaaag tccttgttga
    15901 agcggatcaa gaacatagtg atttaactgg tagtgaacaa gaagcgcatt ttatagtaga
    15961 acaagttaaa gatatcttag aacatcaaaa agtttatgat atgaaaacag gaagctatag
    16021 aagtgcgaca tacaaggata tcgttattct agaacgcagc tttggacaag ctcgcaattt
    16081 acaacaagcc tttaaaaatg aagatattcc attccatgtg aatagtcgtg aaggttactt
    16141 tgaacaaaca gaagtccgct tagtattatc atttttaaga gcgatagata atccattaca
    16201 agatatttat ttagttgggt taatgcgctc cgttatatat cagttcaaag aagacgaatt
    16261 agctcaaatt agaatattga gtccaaatga tgactacttc tatcaatcga ttgtaaatta
    16321 cattaatgac gaagcagcag atgcaatttt agttgataaa ttaaaaatgt ttttatcaga
    16381 tattcaaagt taccaacaat atagtaaaga tcatccggtg tatcagttaa ttgataaatt
    16441 ttataatgat cattatgtta ttcaatactt tagtggactt attggtggac gtggacgacg
    16501 tgcaaatctt tatggtttat ttaataaagc tatcgagttt gagaattcaa gttttagagg
    16561 tttatatcaa tttattcgtt ttatcgatga attgattgaa agaggcaaag attttggtga
    16621 ggaaaatgta gttggtccaa acgataatgt cgttagaatg atgacaattc atagtagtaa
    16681 aggtctagag tttccatttg tcatttattc tggattgtca aaagatttta ataaacgtga
    16741 tttgaaacaa ccagttattt taaatcagca atttggtctc ggaatggatt attttgatgt
    16801 ggataaagaa atggcatttc catctttagc ttcggttgca tatagagctg ttgccgaaaa
    16861 agaacttgtg tcagaagaaa tgcgattagt ctatgtagca ttaacaagag cgaaagaaca
    16921 actttattta attggtagag tgaaaaatga taaatcatta ctagaactag agcaattgtc
    16981 tatttctggt gagcacattg ctgtcaatga acgattaact tcaccaaatc cgttccatct
    17041 tatttatagt attttatcta aacatcaatc tgcgtcaatt ccagatgatt taaaatttga
    17101 aaaagatata gcacaaattg aagatagtag tcgtccgaat gtaaatattt caattgtgta
    17161 ctttgaagat gtgtctacag aaaccatttt agataatgat gaatatcgtt cggttaatca
    17221 attagaaact atgcaaaatg gtaatgaaga tgttaaagca caaattaaac accaacttga
    17281 ttatcgatat ccatatgtaa atgatactaa aaagccctca aaacaatctg tttctgaatt
    17341 gaaaagacaa tatgaaacag aagaaagtgg cacaagttac gaacgagtaa ggcaatatcg
    17401 tatcggtttt tcaacgtatg aacgacctaa atttctaagt gaacaaggta aacgaaaagc
    17461 gaatgaaatt ggtacgttaa tgcatacagt gatgcaacat ttaccattca aaaaagaacg
    17521 catatctgaa gttgagttac atcagtatat cgatggatta atcgataaac atattatcga
    17581 agcagatgcg aaaaaagata tccgtatgga tgaaataatg acatttatca atagtgagtt
    17641 atattcgatt attgctgaag cagagcaagt ttatcgtgaa ttaccgtttg tagttaacca
    17701 agcattagtt gaccaattgc cacaaggaga cgaagacgtc tcaattattc aaggtatgat
    17761 tgacttaatc tttgttaaag atggtgtgca ttattttgta gactataaaa ccgatgcatt
    17821 taatcgtcgc cgtgggatga cagatgaaga aattggtaca caattaaaaa ataaatataa
    17881 gatacagatg aaatattatc aaaatacgct tcaaacgatt cttaataaag aagttaaagg
    17941 ttatttatac ttcttcaaat ttggtacatt gcaactatag tattttgatt ttcaaaagaa
    18001 taaaaaataa tttcgattaa gtgcaaagtc cttgtagcag aatgaacaca actcattttc
    18061 aaaattgtct tacttattta tttgttattt gataacgaaa aaagttataa tgtgaattaa
    18121 gataaagatg aggagttgag aatgaatgaa attcttatca ttcaagtata atgacaaaac
    18181 ttcatatggc gttaaagtaa aacgcgaaga tgctgtatgg gatttaacac aagtatttgc
    18241 tgactttgca gaaggagatt tccatcctaa aacattgtta gctggtttac aacaaaatca
    18301 tactttagat tttcaagaac aagtacgtaa agcagttgta gcagcagaag atagcggcaa
    18361 agctgaagac tataaaattt catttaatga cattgaattt ttaccaccag taacacctcc
    18421 gaataatgtg attgcttttg gtagaaatta caaagatcat gcgaacgaat taaatcatga
    18481 agtggaaaaa ttatatgtat ttacaaaagc agcgtcatct ttaacaggag ataatgcaac
    18541 aattccaaat cataaagata ttactgatca attagattat gaaggtgaat taggtattgt
    18601 tattggtaag tctggtgaaa agattccaaa agcattagct ttagattatg tttacggcta
    18661 tacaattatt aacgatatca ctgatcgcaa agcacaaagt gaacaagatc aagcattttt
    18721 atcaaaaagt ttaactggcg gttgcccaat gggtccttat atcgttacta aagacgaact
    18781 accattacct gaaaatgtaa atattgttac aaaagttaac aatgaaatta gacaagatgg
    18841 taacactggc gaaatgattc ttaaaattga tgaattaata gaagaaattt caaaatatgt
    18901 tgcactacat ccgggagata ttattgcaac tggtacacca gcaggcgttg gtgcaggtat
    18961 gcaaccacct aaatttttac aaccaggtga tgaagttaaa gtgactattg ataatattgg
    19021 aacgctgaca acttatatcg ctaaataatt atcatttaaa aagctaacca gtctttatat
    19081 agattggtta gttttttctt gcttttctaa aaaggtgtta aagataaatt atttataatg
    19141 ttaccatttt gagatgaaag tgaaatattg atattaagaa gtagttgatt attttacagc
    19201 agattcacaa tattctaata aggaataaaa tgtcatgttc ttcctctcaa atatagaagt
    19261 gtggtagaat atatattcgt gtataatcaa atcatgatta aattacaagc aagtgggtat
    19321 taatcccaag aagctttaat ttaataatat aaataattaa agtgggggga atcagtatgt
    19381 tacatttaca tatattaagt tgggtattag cgattatttt atttatcgct acatacttaa
    19441 acatttcaaa aaatcaaggc agatcaccat ttttcaaacc gttgcacatg attttacgct
    19501 tatttatgct gttgacgtta atttcaggat tttggatatt aattcagtca tttatgaatg
    19561 gcggggcaaa tcatatgttg cttacattga aaatgctgtg tggtgttgca gtagttggat
    19621 tgatggaagt gtcgattgct aaaagaaaga gacatgaaca aagtcacaca atgttttgga
    19681 taacaattgc attaattatc atcacaatgg tattaggtgt cattctaccg ttagggccta
    19741 tatcaaaatt attcggtatt ggctaattga taagacatat agtatatgac ataaggcatg
    19801 gacaattatt agtccaagac cttatgtctt ttttgattct aaaaatgtta aacataaaaa
    19861 atggactgga tagtagtacc agtccaacat aattctaacc tagacaatta agattagatt
    19921 gaatcgcatg attcatttat ttagctttgt aaccaatcat attgattaaa tctttagggt
    19981 ggctatatgg tggtgcataa gccacttcaa actcagttaa ctcatctaca gttagctggt
    20041 tcatcattgc catcgatagt acatcaatac gtttatctgc accttctttt cctactgcag
    20101 ctgctcttaa aatctgacgg tttgaagtgt cataatatac tcttaagtgt aaaggggaat
    20161 ttcctgggta ataattcgcg tgtgcacctt gagtgacttc taccatttta tagtcaaatt
    20221 gctttagttc gtttggttta acgccgacac tcgcaaatgt ataatcaaag aacttcacaa
    20281 tattgttgcc taagaagcct ttgaattcaa tagtgtcatt tccagcaatt tgttcggcaa
    20341 caatacttgc tgcacggtga gcgccccaag ctaaaggaac actagccggt agatcgacat
    20401 gtcgataatg tgatgttgca atatcgccta ttgcataaat gtttggaaca tttgtttcaa
    20461 atttatcgtt taccggtatg aaaccttttc gatcaagttt gatatttgaa ctttcgataa
    20521 attttgaatt ggggtgagta ccgacacctt caataatcat atcgtaatgt tcaacttttc
    20581 ctgatttaaa tgtaatttca tttccattga tagcattaat ttcctcattt aaacggtatg
    20641 gaatctcccg cttatctaat tcatcaagta taggttgatt catgtcggca tccattaatt
    20701 tatttatctt atcagatcga tgaattaaag tagggtgtaa accacgttca taaagatttt
    20761 caagaacttc taatgaaaca taacctgcac ctacaaccaa tactttatca acttgatttg
    20821 ctttgatgaa ttgatcgata gcatcagtgt cttctaaatt tctaagtgta aatgtaatat
    20881 cactttcaaa gccaaggcta tttgcacttg caccagggct taaaatgagt ttatcgtaag
    20941 attcttcaaa ttgttcgttt gtctttctat ttaatacaga tacagtttgt ctttcatcat
    21001 tgattgcaat aacttcatga taagttttta ctgtaatttg ctttctatca taaaattttt
    21061 caggtgtata cgctaaagca tatcttctat cttcaacaac ttcgccaatg acataaggca
    21121 atgcacaatt agcaaagctc atatcacgat ctttttcaaa aataataatg tcactttctt
    21181 tatctaaacg tcgaatttgg ctggcacatg ttgcaccgcc agcgactgct ccgactacga
    21241 ctattttggg catcgtaact tacctcctat tgttttaaaa tattataaaa tgattagtta
    21301 taaattaaca gtaatatcta atatttaaat taaagaaatc attcaaatat cgaccaatgc
    21361 catcttcatt attgttgaat gtaatattgt tcgctacatc tttaagttct tgcaaaccat
    21421 tttccatagc aacaccgtga cgcgcgtact caatcatttc aatatcatta tcttcatcac
    21481 cgaatgcaat aatattattt cggtcaatat ttaaaaattg tctaacttgc tcaatgcctc
    21541 ttgctttatt aataccaagt tttacaattt caatgacagg gaatggtgcg ccccagcgtc
    21601 gatgctcaat atgatcggca taaaaatgag taagcatatt tttgatttca ggtattttac
    21661 tttcttcggc ttcaattaaa attgaggtag gggattcttt caagtggaca agtaaattac
    21721 cagtttgaat tcttggatta cccattgaaa aaccttcaaa taatcttgga tcatgattgt
    21781 taatgaaaac ataatctttc acttctgcta taatattcga tacttgatat tgttgtaatc
    21841 cttgaataat gttttgtgcg atgcctaaat ctaaaatttc atggcaagtt ttgaagtttt
    21901 tatctttagg gtgatgtacg taagcgccat taaaattaac aattggtgtc gttaaattta
    21961 attcatgata atacatttga cttgcacgat aaggtctgcc agtcgcaatc ataatttggt
    22021 gtccacgttg ttgtaattca tttaatactt gtttagtata tgatgaaatt tctttgttat
    22081 cgtttaataa tgttccgtct aagtctagac atattaaatg tggttgcata tctctctcct
    22141 cataagttgc ttagttcata tagatacatc atagcatttt attggcgttt tgtcggatat
    22201 ttgatatatt atgaataata tgacattaaa gaggtgatat ggatggaaga ggcattgaaa
    22261 gatagtatct taggtgcatt agaaatggta attgaccctg aattaggaat tgatatcgtt
    22321 aatttgggtt tagtatacaa agtgaatgtt gatgatgaag gcgtatgtac agttgatatg
    22381 actttaacat caatgggatg tccaatggga cctcaaatta ttgatcaagt taaaacagta
    22441 ttagcagaga ttcctgaaat tcaggatact gaagtgaata tcgtatggag tccaccttgg
    22501 acaaaagata tgatgtcacg ttacgctaag attgcacttg gtgtgagcta acataaactt
    22561 gcattgcctg tagaatttct tttcgaaatt ctctgtgttt gggcccgcag tcaatataac
    22621 attgtagagt ataattcatt gtgaataagc tcccttcaaa gtagacattg aaaaatgaaa
    22681 attttgaagg gagccttatt tatgttctag tttctgattt tattttgaaa atttatatct
    22741 aacttgtttc aaaaaatatg aattaagaca gtgacttgca cttccaagag tgcacaattc
    22801 attttctagt tgaatatttt cttgaaaatt tttataataa atttaattta tattacagtt
    22861 atattacaat acataatact tcatagtttt taagttgccg agttgcatta caaattggat
    22921 ttcgaagtaa gaatcatcat tggtaatttt tttaatttaa cagtatttta gtcaatatga
    22981 ttttgatgat gtcgtaattc aataatatac atatgcattt caatattctt ttaaacagca
    23041 taactaaatt taattaagca tctaagaaga acagttttaa aagggaagaa tgcaaaaatg
    23101 aacaaaacaa agggttttac aaagtataag aaaatgagat atattccagg gctcgatggt
    23161 ttgagggcaa tcgctgttct aggaattatt atttaccact taaataagca atggttgaca
    23221 ggtggctttt taggtgtgga tacatttttt gtgatctctg gttatttaat tacaagctta
    23281 ttactcaaag agtatgatga cacaggtatc attaaattga aaagcttttg gatacgtcgt
    23341 ttaaaacgtt tattaccagc agtcatagtt ttattaatgg ttgtagggac agcaacctta
    23401 ttattaaaat cagataatat cattagggtt aaacatgata ttattgctgc gatattttat
    23461 gtatcaaact ggtggtatat agcaaaagat gttaattatt ttgagcaatt ttcatttatg
    23521 ccattaaagc atttatggtc tttagcaatt gaagaacagt tttacatatt tttcccagtt
    23581 attttggtta cattattgtt aacaattaaa aagcgataca aaataggatt tattttttgg
    23641 ggagtatcaa taatttcttt agggttaatg atgtttatct atagtattaa tggggatcat
    23701 tcacgagtgt attttggtac agatactaga ttacagacat tgttactggg tgttatttta
    23761 gcttttttat ggccaccgtt taaattgaaa aatgatccac ctaaagttgt aaaatatgtt
    23821 attgatagca taggtagttt atcatttata gtacttatat tattattttt cattattaat
    23881 gatgagacga attggatata tgatggtggt ttctatttaa tatccatatt aacgttattt
    23941 attattgcta gtgtcgttca tccatctaca tggatagcga agatattttc aaatccagtg
    24001 ttagtattta tcgggaaaag gtcttatagt ttatatttat ggcattttgc agtaattagt
    24061 ttcgtacata gttactatgt agacggacag atacctgtat atgtgtactt tatagatata
    24121 agtttaacaa ttatatttgc agagctatca tatcgcttta tagaaactcc atttagaaaa
    24181 gaaggtatta aagctttaaa ttggcgacct tcttatatac cacaatttat aagaatggca
    24241 attgtagtaa ccttgttaat tccatttatg ttgattttag taggtgcatt caataaatat
    24301 ggtaaagaca ttattggaga aaaagcgaat agctttgata ccacaattga agataattat
    24361 ttaatgcgga tagcaccaat tgataacata cacattgatg gcttagtaag tgagaagaaa
    24421 aaggaatctt ccgacgtata taataatatt aaacctcttt taatcggtga ttcagtaatg
    24481 gttgatatcg gtgagtcatt taagtcatca gttcctaagt ctagaattga tggaaaagta
    24541 gggcgtcaat tgtatcaaac cttaccttta gttaaagcga attattcaca atataaaaaa
    24601 tcatctgatc aagtcgtatt agaattaggt acaaatggcg actttactgt caaacagctc
    24661 gacgatttac ttaatcaatt tggaaaagcc aagatttatt tagttaatac acgtgttcca
    24721 agaatttatg aggcaaatgt aaatcgatta ttagctgacg cggcgaaacg aaagtccaat
    24781 gtcacattaa ttgattggta taagcgatca caaggacata gtgaatattt tgcaccagac
    24841 ggtgtacatt tagagtacaa aggagtccta gctttaaaag atgaaatatt aaaagcactt
    24901 aaaaagaaat aacatatgtt taataagtct agttaatgtg taacgtaaca ttagctagat
    24961 ttttttattc aaaaaaatat ttacaaatat taggaaattt aagtgtaaaa gagttgataa
    25021 atgattatat tgggactata atataattaa ggtcaaagaa agtcaaagtc aaaagtgcaa
    25081 cttgaccaac tctacaaatt agaggtgaaa ttatatggat ataaataaaa tgacatatgc
    25141 tgttcaaagt gctttacaac aagcagttga actgagtcag caacataaat tacaaaatat
    25201 agaaattgag gcaattttaa gcgctgcctt aaatgaaagt gaaagcttat ataaaagtat
    25261 tttagaacga gcaaatattg aggtagatca attaaacaaa gcttatgaag acaaactaaa
    25321 cacgtatgca tctgtagaag gtgacaatat acaatatggt caatatatta gccaacaagc
    25381 aaaccaattg ataactaagg ctgaatcata catgaaagaa tatgaagatg aatatatttc
    25441 aatggagcat attttacgtt cggcaatgga cattgatcaa acaacaaaac attatataaa
    25501 taataaagta gaagttatca aagaaattat taaaaaagta agagggggaa atcacgtgac
    25561 atcacaaaat ccagaagtta attacgaagc attagctaaa tatggccgcg acttagtaga
    25621 agaagttaga caaggtaaaa tggatcctgt tataggaaga gatgaagaaa ttcgaaatac
    25681 gattcgtatt ttaagtcgta aaactaaaaa caaccctgtg ctcattggtg aaccaggtgt
    25741 tggtaaaact gcaattgttg aaggattagc gcaacgtata gttaagaaag atgtgccaga
    25801 atcattatta gataaaactg tttttgagtt agatttaagc gcattagtag cgggcgctaa
    25861 atatcgtggt gaatttgaag agagattaaa agcagtccta aaagaagtta aagagtctga
    25921 tggtagaatt atattattta ttgatgaaat ccatatgctt gtaggtgctg gtaaaacaga
    25981 tggtgccatg gatgcaggca acatgctaaa accaatgtta gcacgaggag agttacattg
    26041 tattggtgca acaactttaa atgaatatcg agaatatatt gaaaaagatt cggcattaga
    26101 gcgtcgtttc caaaaagtag cagttagtga gcctgatgtt gaagatacaa tttcaatttt
    26161 acgtggttta aaagaacgat atgaagtgta tcatggtgtg cgtattcaag atagagcctt
    26221 agttgctgcc gctgaattgt ctgatcgtta catcactgat cgttttttac cagataaagc
    26281 gattgattta gttgaccaag catgtgcaac aattcgtacg gaaatgggat caaatccaac
    26341 tgaattggat caagttaata gacgtgtcat gcaattagaa attgaagaaa gcgcacttaa
    26401 aaatgaatct gacaatgcga gcaaacagag attacaagaa ctacaagaag agcttgccaa
    26461 tgaaaaagag aaacaagcag cacttcaatc tcgtgtagaa tcagaaaaag aaaaaatagc
    26521 aaatttacaa gaaaaacgtg cgcaactaga tgaaagtaga caagcgttgg aagatgcaca
    26581 aacaaataac aatttagaaa aagctgctga actacaatat ggaacaattc ctcaattgga
    26641 aaaagaactt agagaattag aggataattt ccaagatgag caaggtgaag atacagatcg
    26701 aatgattcgt gaagttgtaa cagacgaaga aattggcgat attgtcagcc aatggacagg
    26761 cataccagtt tcaaaattag ttgaaacaga acgtgaaaaa ttacttcact taagtgacat
    26821 cttgcataaa cgtgttgtag gtcaagataa agcggttgac ctggtttcag atgcagtagt
    26881 tagagcaaga gcaggtatta aagatccaaa cagacctatt ggtagtttct tattcctagg
    26941 tccaactgga gtaggtaaaa ctgaattagc taaatcatta gctgcatcat tatttgattc
    27001 tgaaaaacat atgattcgta ttgatatgag tgaatatatg gaaaaacatg cagtatcaag
    27061 attgataggg gcacctccag gatatattgg acatgatgaa gggggtcaat taactgaagc
    27121 ggttcgtcgt aatccatact cagttatttt attagatgag gttgaaaaag cgcatactga
    27181 cgtctttaat gtattattgc aaattttaga tgaaggccgt ttaactgatt ctaaaggacg
    27241 tagcgttgat tttaaaaata ctattattat tatgacaagt aatattggat ctcaagtttt
    27301 attagaaaac gtaaaagaga ctggtgaaat tacagaatca acagaaaaag ctgttatgac
    27361 aagtttaaat gcatatttca aaccagaaat tttgaatcgt atggatgata tcgtattatt
    27421 taaaccatta tctattgatg acatgagtat gattgtagat aaaatcttaa cgcaattaaa
    27481 tataagatta ttagaacaac gaatctcaat tgaagtttct gatgatgcta aagcttggct
    27541 aggtcaagaa gcttatgaac ctcaatacgg tgcaagacca ttaaaacgtt ttgtacaacg
    27601 ccaaattgaa acaccattag cacgtatgat gattaaagag ggattcccag aaggtacaac
    27661 gattaaagtt aatttaaatt cagacaataa cttaacgttt aatgttgaaa aaattcatga
    27721 ataaaattga accaagaatg tgatgattaa tcgcattctt ggttttttag atttgaaatt
    27781 tattataatt gttcattggc aatatacttt ttaaatgatt taataaacac caatatttct
    27841 gggctttctt ttttgctgta tatatatgta aatgaaatag gtgcttgtaa aattttcgtg
    27901 ttaataaccg aaatattgta atcactattt gttgttatat aaagaggcag gaatgatata
    27961 ccttgattca tttcgattaa tttaattgaa gtatgcacat cattgataga tagaaattgt
    28021 gctttttcat aaatatttaa aatattattt ttaagtgatg accaatattc tggatggtta
    28081 tcacttatta ttttgtattt ttcaaataaa gatgcctcag ttagaagatg attattctct
    28141 ttattgggag caatcaatac aattttacct tcgcatactt tttcagaatg aacttctctt
    28201 agtttaggtt gatttctgct aatcccgatg tcatacgtat gattattaat atctttttca
    28261 atattttcat ttttgacatg aagagaaaca tcgataaaag gatgctcgtt aaagaaagat
    28321 tttaaaaatt tgggcataat gaatgtcgcg atatatgaag acacgacaac atttaatttc
    28381 gattgaaaca tcgttttttt aagttggata tgtttgatgc cactttcata ttgttcaata
    28441 aaactttgcg caattggaag aaatgtatga ccatcttcag tcaagataat ttgattttta
    28501 taagtttcaa atagtttcac attgagatgc tgttctaaat ttttaatttg cttatgtata
    28561 gaaggtatag tgagattaat ttcttcacta gctaatcgat agtttaacgt cttcgctaaa
    28621 atgacaaatg tatagtacca atctaaattc atgaacttac ctctttcaaa aaagttaata
    28681 attcattaga taatatttaa tttttattgc ataaaaataa tattaatatt atatcagagg
    28741 ttatcgaggg ggtgaagtaa tatgattcga attcaagata atacaataag ggatggtatg
    28801 caacaaagta atgttcgaaa aagtctaatt ataaaaaaag aagtattgaa acaaattaac
    28861 aagttaaata taaattctgt tgaagtaggc atgtgtacaa ctatcgagga tgaatttaat
    28921 attcatcaat tcagagacat tttaagtcct gaaaaagaat tagtagtatt gactaggctt
    28981 aatgaaaaag aaataaaaaa aatagtcaaa ttgaaaattc ataatttagt ggtaaaaata
    29041 ctattgccaa tatctgactt gcatataaaa gaaaagctta atttttcaaa taaatattat
    29101 attcagaaaa tcaaagactg cttggatata ttaaagaaag ataaaaaagg agtagatatt
    29161 tgttttgaag atggaacaag gacttctaga gaaaaattga aagaatacat ggaaattatt
    29221 tcaaaatatc aagttagaac agttacattt gcggacactg taggatgttc gacaccatta
    29281 gaatacggag atatttttaa ttactttgta aaaaaatatt ctaacataat tttttctgct
    29341 cattgtcata acgatctagg gttggctact gcaaatacat tagctgcaat tttaaatggt
    29401 gcaaagcaaa tagaaactac atttttgaga attggtgaga gagcgggtaa tgctcctatt
    29461 gaggaaataa ttactatttt gacaaaaaaa caaatagaaa gtacggaatt cactttaccc
    29521 gacgtatata aaactagtat taatatttct aaaattctcg attttcaaat atcagaaaac
    29581 aaacctataa ttggtgaaaa tatatttaaa catgaatcag gaattcatca agatggtact
    29641 aaaaaaaata taaatatgta tcaatattta gttcctagtg atttaggatt gaaaaattca
    29701 caagttgttc agtttccaat aagtaatatt tctagtaaga aaatcttgca caataaattt
    29761 aaatcaatag ttaacactga agaaattgat gaaaatattt ctttctataa acttgtgaac
    29821 aagttttcac ctgaagtagc acctgaagat acagtggatt tacttcagat aataaaaagg
    29881 aggagtaaag atggaaattt taaatgattc tgtaaaaagt tttgaaaaac tttatttaca
    29941 attacaagat ggttttccag tgattgttcc tactgatact aattataatt tatgtagttt
    30001 acctaacaac gatctttgta tcgataaaat atttgaatat aaaaagcgat cgaaagataa
    30061 gccattatca ttatttattg ataagccaga ggattggaaa ttgtatggag ataatcaaaa
    30121 tacggaaata gttgataaac tagttgaaat attttggcct ggaccattaa atattatttt
    30181 aaaaaataaa acaagctata attatatgct caataattca gatagtatag ctataggatg
    30241 tgtacaaaat aaaacgatga gaagatttat ttcatatatt aattcaccta tagcaattac
    30301 ttcagcaaat atatctggaa ctgccgatga tattttaata actgaaaatg aagcaattaa
    30361 gcacatgggc gaaaatgtaa gatatatgct aagaagtcaa aataaaacta actataaaac
    30421 atctagtacg attattaaag tgacagataa taaaattgaa ttattaagag aaggagatat
    30481 aaagtttgaa gaaataaaag aaagactagg tacaggtatt atttatgaat aagttaatac
    30541 ttgggattta tttataccga attttttcac gagcatactt ttatttaccg tttttattaa
    30601 tttacttttt gattcaaggt tattccataa tacaattaga aatattaatg gcgtcttatg
    30661 gcattgcagc atttttattc tctctataca aagagaagtg ttttaaaatt tgtaacttaa
    30721 aagattcta
//
LOCUS       DALAGF010000021        28822 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.021, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000021 DALAGF010000000
VERSION     DALAGF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28822
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.021"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            61..390
                     /locus_tag="O2692_000900"
     CDS             61..390
                     /locus_tag="O2692_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298538.1"
                     /translation="MAYSILNLYSILIQYKCKINHKKDIQYLHPRKTLQLPLILKIIL
                     NLALIGIVIIVLTHTEFQLNAGDSNVFLLNWDIRGLTSYIFSFLILVALVSKLILIFI
                     TRRQRTS"
     gene            complement(450..2048)
                     /locus_tag="O2692_000901"
     CDS             complement(450..2048)
                     /locus_tag="O2692_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCZ4298539.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
     gene            complement(2242..2685)
                     /locus_tag="O2692_000902"
     CDS             complement(2242..2685)
                     /locus_tag="O2692_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCZ4298540.1"
                     /translation="MKIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLRRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            complement(2966..3178)
                     /locus_tag="O2692_000903"
     CDS             complement(2966..3178)
                     /locus_tag="O2692_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCZ4298541.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            complement(3456..5165)
                     /gene="betA"
                     /locus_tag="O2692_000904"
     CDS             complement(3456..5165)
                     /gene="betA"
                     /locus_tag="O2692_000904"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCZ4298542.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            complement(5426..6916)
                     /gene="betB"
                     /locus_tag="O2692_000905"
     CDS             complement(5426..6916)
                     /gene="betB"
                     /locus_tag="O2692_000905"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCZ4298543.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            6985..7080
                     /locus_tag="O2692_000906"
     CDS             6985..7080
                     /locus_tag="O2692_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298544.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            7169..7732
                     /locus_tag="O2692_000907"
     CDS             7169..7732
                     /locus_tag="O2692_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HCZ4298545.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINSLLESSDLTEAETQEAQKIKSQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(7920..8606)
                     /locus_tag="O2692_000908"
     CDS             complement(7920..8606)
                     /locus_tag="O2692_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCZ4298546.1"
                     /translation="MTNQFKNKQSKLHDSLESITKNLYATPTSELPFDNRFLFKSFIL
                     KRETGNIVIYHSGHLGDSQQDIASLGGVSKVLMNHDHESIGGSNQVEAPYFIHENDVA
                     ALKHKISVQKQFSNRVMLDKDLEVIPAPGHTPGTTLFLWDDGHHRYLFTGDFICFEGK
                     RWRTVILGSSDREKSIQSLEMVKELDFDVLVPWVTIKDEPLVYFVENEYEKREQIQNI
                     IDRVREGENS"
     gene            complement(8870..10492)
                     /locus_tag="O2692_000909"
     CDS             complement(8870..10492)
                     /locus_tag="O2692_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCZ4298547.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            10624..10785
                     /locus_tag="O2692_000910"
     CDS             10624..10785
                     /locus_tag="O2692_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298548.1"
                     /translation="MAIIEYLYNFSRNKQNLNSAKITVLSYDLTNLTNFTRMAFKNIY
                     TLLTNIYLL"
     gene            complement(10801..10911)
                     /locus_tag="O2692_000911"
     CDS             complement(10801..10911)
                     /locus_tag="O2692_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298549.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVEREKWYFSGKEV"
     gene            complement(11011..11547)
                     /gene="nrdG"
                     /locus_tag="O2692_000912"
     CDS             complement(11011..11547)
                     /gene="nrdG"
                     /locus_tag="O2692_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCZ4298550.1"
                     /translation="MILLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            complement(11544..13394)
                     /gene="nrdD"
                     /locus_tag="O2692_000913"
     CDS             complement(11544..13394)
                     /gene="nrdD"
                     /locus_tag="O2692_000913"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCZ4298551.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            complement(13641..15011)
                     /locus_tag="O2692_000914"
     CDS             complement(13641..15011)
                     /locus_tag="O2692_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HCZ4298552.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            complement(15333..15428)
                     /locus_tag="O2692_000915"
     CDS             complement(15333..15428)
                     /locus_tag="O2692_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298553.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKDT"
     gene            complement(15504..16109)
                     /locus_tag="O2692_000916"
     CDS             complement(15504..16109)
                     /locus_tag="O2692_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HCZ4298554.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGVVASRRAQTLNQYVEHMTVISPTI
                     TEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKKALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR"
     gene            complement(16172..18052)
                     /locus_tag="O2692_000917"
     CDS             complement(16172..18052)
                     /locus_tag="O2692_000917"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HCZ4298555.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEED
                     AEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLIFMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESKRF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRD
                     VY"
     gene            18247..18339
                     /locus_tag="O2692_000918"
     CDS             18247..18339
                     /locus_tag="O2692_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298556.1"
                     /translation="MSVINAYSNASLLFFIYLLFIMYQISLSVK"
     gene            complement(18447..18944)
                     /locus_tag="O2692_000919"
     CDS             complement(18447..18944)
                     /locus_tag="O2692_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCZ4298557.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(18954..19214)
                     /locus_tag="O2692_000920"
     CDS             complement(18954..19214)
                     /locus_tag="O2692_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298558.1"
                     /translation="MIIMRGYNGRYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
     gene            complement(19596..19709)
                     /locus_tag="O2692_000921"
     CDS             complement(19596..19709)
                     /locus_tag="O2692_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298559.1"
                     /translation="MNGILIKCLNIGGQYYLQTRMLSNVDNAEKIISRNSI"
     gene            complement(20368..22368)
                     /locus_tag="O2692_000922"
     CDS             complement(20368..22368)
                     /locus_tag="O2692_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4298560.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFVIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDSVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNHHIFLTSYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
     gene            complement(22365..23120)
                     /locus_tag="O2692_000923"
     CDS             complement(22365..23120)
                     /locus_tag="O2692_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298561.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            complement(23228..24115)
                     /gene="nsaS"
                     /locus_tag="O2692_000924"
     CDS             complement(23228..24115)
                     /gene="nsaS"
                     /locus_tag="O2692_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HCZ4298562.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            complement(24126..24791)
                     /gene="nsaR"
                     /locus_tag="O2692_000925"
     CDS             complement(24126..24791)
                     /gene="nsaR"
                     /locus_tag="O2692_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HCZ4298563.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQNQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            complement(24817..25017)
                     /locus_tag="O2692_000926"
     CDS             complement(24817..25017)
                     /locus_tag="O2692_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298564.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQDKEQKDNKG"
     gene            25215..26639
                     /locus_tag="O2692_000927"
     CDS             25215..26639
                     /locus_tag="O2692_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCZ4298565.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            26709..26819
                     /locus_tag="O2692_000928"
     CDS             26709..26819
                     /locus_tag="O2692_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298566.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(26922..27377)
                     /locus_tag="O2692_000929"
     CDS             complement(26922..27377)
                     /locus_tag="O2692_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCZ4298567.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(27622..28383)
                     /locus_tag="O2692_000930"
     CDS             complement(27622..28383)
                     /locus_tag="O2692_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCZ4298568.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNSDTTVKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRINVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            complement(28499..>28822)
                     /locus_tag="O2692_000931"
     CDS             complement(28499..>28822)
                     /locus_tag="O2692_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCZ4298569.1"
                     /translation="DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSRVTPPNNEQK
                     APSNPKGEVNHSNKVSKQHKTDALPETGDKSENTNATLFGAMMALLGSLLLFRKRKQD
                     HKEKA"
BASE COUNT         9182 a         5346 c         4172 g        10122 t
ORIGIN      
        1 aatatcaaat caatcataat tatttgacaa caaatagcta acgattgttt taatctacat
       61 ttggcttata gcattttaaa cctatactct attttgatac aatataagtg taaaatcaat
      121 cataaaaagg atattcaata tctgcatcca agaaaaacat tacaattacc tttaatattg
      181 aaaattattt tgaatcttgc acttataggt attgtaatta tcgttctaac acatactgag
      241 tttcaactta atgccggtga tagtaatgtg tttttattaa actgggatat tcgaggttta
      301 acatcttata ttttctcatt cttaatactg gttgcgcttg tttctaaact aattttaata
      361 tttataacta gacgacaaag aacatcataa ataaacaagc agtccagtgc gtatcatcat
      421 accttacgca ttggactgct ttgctatgtt tattgattgc tatctttttg ttgttgccat
      481 ttttctattt cagcatctcg tagttcaaca cgacgaattt tacctgagtt tgtttttggt
      541 aagtcgtcaa cgaattcaat ctctctcgga tatttatatg gtgcaacttc atttttaaca
      601 aattgttgta gttctttaac taacgtatca tcacccgcag tatggtcctg taaaataacg
      661 aatgctttaa caatatttcc tcgtatatca tgaggttttg ctactacagc acattcttta
      721 acggctggat gattcgttaa cgcatcttca acttcaaaag gcccaatcgt atagcctgaa
      781 ctaataataa tgtcatctcg acgtccttca aaccagaaat aaccatcatc atctacatga
      841 gctaagtcac cagtgatgta gtatttacct gtttgcgctt tcgccgtacg ttctggctct
      901 ttataatacc ctttgaaaag tgctggcaaa tcaagtggta ctgcaatatt ccctttcgta
      961 ttagcaggta cgctattccc ctcatcatct actacagtga ccgaactacc cggaatgcct
     1021 ttacccattg atccaatcct ctgtggtgta tcttttaaaa agcctataag caaggtactt
     1081 tcagtctggc catatccatc tcttacagtt aaattaaagt atttcttgaa ttgttcaact
     1141 acttctcgat ttagtggctc acctgcagaa acggcactat gtaaatgcgt taagtcataa
     1201 tcatttaagt tctgtaattt agccatcata cgatattctg tcggtgtaca acataaaaca
     1261 ttaatttgat atttttgaag caattctaag tatgtttcag gactgaacct tccattaaat
     1321 acaaaagcag ttgcacctga acctaataca gataagaaag gactccatac ccatttttgc
     1381 caacctggtg ctgctgttgc ccaaactaag tcatcttcat taatacataa ccaatgtttt
     1441 ggtgccattt gtaaatgtgc aaatccccat ccatgacaat gtgtaacggc tttaggattg
     1501 ccagttgtac cagatgtata tgacagaatc gccatatcat cacgcgtcgt atctgccatt
     1561 tctagtttgt tacttgcgtt ttctttttca gcttcaagtg aaatccatcc atctttttga
     1621 ccggcaataa caaatttagt taacgcatca tattctttaa ttttttcaaa ttcaactgtg
     1681 aatggctcta gtgcaataac tgcattaatt tcaccatgtg tgatacggta ttgtaaatct
     1741 ttagttctta gcatttcaga acatggaatg attgcaacac ctaattttaa agcagcaata
     1801 tataattcat acgtcgcaat agatcgtggc atcataatga gtactttatc gcctttagat
     1861 aaaccgtgcg atgctaaaac attacctact ttattagact gttcaatgag ctgttggtaa
     1921 gtgactgata tatcttcgcc ttcagtatta tgatataaaa ttgccttttt atctggtatg
     1981 tggctatatt tttcgatttc cgaaataatg ttatattttt caggcgcgaa tagagctgac
     2041 ttttgcataa ctaacttcct ttcatacatc cacttttcct gtgatgaaca ttgtaatttt
     2101 ataaatgaat tatatacatc atacgcctat ctttacagaa ttttcaatta aatagggtta
     2161 aataccaaag tcctcgacac tacactttga catgacgtag cattcaagga ctttcaaatg
     2221 attgagggtt gatatctcgg gctagaccat atcagctaat tcaatacgaa tattgtatga
     2281 taattcacga ttaattattt ttacatctgc acctttcgaa gtgccacgat gcttgtgtgt
     2341 atgcttgtac tcagctgaat tttgccaatg ataaaatgct tgcctatttt cccacagcgt
     2401 aataatgata tagtgtctac cagctgttct aggtcttaaa aaccttaatg ctttaaatcc
     2461 atcaacgttt tttaaatgct tattacgtcg caaaaatttt tgctcgaatg tttcttcttg
     2521 tccttcgtta atatataaat gatttaacac acataatgaa tcatttgata aatcatttat
     2581 cgcttctagc acatcgtaat atgcagtgtc attatttttt tgtattgtga gacaatcatc
     2641 cagttcttct attacataac ttctatattc atcataaatt ttcataataa atgccttcat
     2701 ttcatttata tttttggtca tattacttta tatctattac taattcattc ccgtatttat
     2761 taattacaat catagtttgg cttcttttta aaagataaga ctttgtaaaa agtattaata
     2821 tttcatgcaa atgggggaca ggagtcgccc actatttttg tgtcttcaat ttcatgatca
     2881 ttatttaaca ttagtcatga aaatagccga caacttcatc tgtgaaatca ccggcctttt
     2941 attttagcta actttatttc tgattttacg attttaattg atcatacaga gaaagtgatc
     3001 tttttacaat ttctaaaaac tcatgatcta tattggacat ttgatgaaaa taagacaaaa
     3061 tgttttctgt tagcttctct tgttttggga atgaatcatc ttctttaatc caaatcgcta
     3121 attcgcctaa tggtgtttta tcatctttaa atgtttgtat atattcgtaa aagctcatag
     3181 tattccttct ctcaatttac ttatataaat cctaccacga aagctttcaa gaaaacacaa
     3241 ttaaatgtct atttagtgaa ctttttaagg ttgtgcactc ttttaatgtc tgccaattag
     3301 gtcaattaat catcacaatg tacaattaac tctattttca gttcatatac tcacacaccg
     3361 tttttgaaca acacattaac ttctcattta gataaaacgc aaaaaagcct ggcaccaata
     3421 caatagatgc cagactaaga gtctactata taaatttatt tagcgtatgg ttttacttcg
     3481 attgcacctt cattttcatc atgaacacca tgcttataat aatcaatata ttgtggctct
     3541 aaaggctttc tgccacgtat aatgtctgct gctttttcag ctaacattaa aacaggtgcg
     3601 tgtatattgc catttgtcgt acgtggcata gctgatgcat caactacacg taaattttcc
     3661 ataccgtgga ctttcattgt taacgggtca actactgcca ttggatctga agcaggaccc
     3721 attttagcac tacaagatgg gtgtaatgct gtttcaccat ctctacgaac ccaatcaaga
     3781 atttcttcgt ctgtttgcac ttctggtcct ggtgaaattt ctccaccatt gaatggatcc
     3841 attgcttttt gagataagat atttcttgct acacgaattg cttctaccca ttctttttta
     3901 tcttcttctg ttgataaata attaaagcgg atacttggtt tttcgaatgg atctttagat
     3961 ttgattttca agctaccacg agagtttgaa tacattggtc ctacgtgaac ttgataacca
     4021 tgtgcgaccg ctgccttttg accatcatat cttacagcta ttggtaagaa atggaacatt
     4081 aagttaggat aatcaacttc gttatttgaa cgtacaaatc cgccaccttc aaaatggtta
     4141 gatgctgctg cacctgtacg tgtgaaaatc cattgtaaac caataaatgg catgcgcttg
     4201 atatctaagc ttggctgtaa tgatacaggt tccttacatt tatgttgaat gtatacctct
     4261 aagtgatctt caaagttttc acccacacca ggtaaatgaa cacgtggctc aatgcctttt
     4321 gattttagga actctgaatc accgatacca gataattgta gtaattgtgg cgtattgaat
     4381 gccccaccag acaaaatgac ttcattagca tcgatggtat gtagtttgcc atttttctta
     4441 tacgtaacac cagttgctct tctaccttca taatgaattt cagttacaaa ggcacgtgtt
     4501 tcaacggtta agtttttacg cttcatcgct ggatgtaaat atgctcttga agctgacatt
     4561 cggcgaccac gatgtacttg actatcgaac ggtccaaaac cttcttgtct aaatccattc
     4621 acatcaggtg ttttatgata gcctgcttca acacctgcat caaagaatga ctggaataaa
     4681 ggattcgttg ctggccctcg ttttaactta attggtccat catggcctct aaatttatca
     4741 taaggcgctg caccgtatgt tttttctaat tttttaaaat acggtaaaca gtgcgcaaaa
     4801 tcccaagttt ccataccttc tggttctgcc cagccttcat agtccattgg attaccacgt
     4861 tgataaatca tgccattaat cgaacttgat ccacctaaaa ctttacctcg ggcatgtgct
     4921 actttacgac cgcccatatg tggttcttca tctgttgaat aaatccaatc gtaaaattta
     4981 ttgcctgaag ggaacattaa cgcagcaggc atttggataa ataaatccca aaaataatca
     5041 ctgcgacccg cttctaatac taagacttct ttatctttat cttcactcag acgattacct
     5101 agtacagaac ctgcactgcc tcctccaatg atgacataat catatgattt gtttttgtta
     5161 ctcatcgtta tgacctccaa agtttgttgt atttcaaagt gagtattgca tgaaatattt
     5221 gttagtggct gattggttat cgttgaattg ctaaacttag ctaaccaaaa cactcgcttt
     5281 tccttttacg attgttacta acagtaatga aataatctat gattcatttt gcgtttagtc
     5341 caaattatca atcaacattt cgccacttat acaaaacaaa gtgagtgtct tgtgttctta
     5401 caatggcact caccttatct aatttttatt tgctaaacca attcactaat tgtggatttg
     5461 tatttgttaa aatgtgtttt gaaacaaggt actcttctaa gccttctttg cctaattctc
     5521 taccgatacc tgattgtttg tatccacccc atggcgcttg tgcaaaatat ggatggaaat
     5581 cattaatcca caccgttcca agtttcaact tgttagcaac gcgttgtgct tttccaatat
     5641 ctttagaaaa tacagcacct gctaaaccat atatagaatc attcgctaat tgaatcgctt
     5701 cttgttcagt ttcaaagcct tctacagtaa cgacaggtcc gaaaacctct tcttgtacaa
     5761 tacgcattga cgtatcacaa tttgtaatga ctgttggctc gaagaataga ccatctttta
     5821 aatcatctct atctggacgt ttaccaccaa cagcaattgt tgcgccttct gctttagcta
     5881 catccatata agattcgatc ttattacgat gttctgttga aatcactggt cccatttcag
     5941 tatcagcatc aaaaccatta cctaatttga tttttttcac gcgatcaata agtgcttgct
     6001 caaatttgtc tttaatactg ttttgtacta atattcttga tcctgctgaa caaacttgac
     6061 ctgcatggaa atatccacca tttaacgctt ggtctactgc caattcaaaa tcagcatcat
     6121 caaagataat gtttggattt ttaccgccaa gttccaaggc aatattcgta acattattag
     6181 cagcattttt cataatatgc ttaccagtct caatgccacc tgtaaatgat acaaggtcaa
     6241 cctctttatg acctgacatt acgtcaccaa cttcagaacc tgcacctaga ataagattaa
     6301 ttgttccttt agggaaacca acttcttcca ttaattcaaa aacacgtatt gttgttaatg
     6361 gtgtaatttc acttggtttc ataactagtg aacaacccgt agcaagcgct ggcgcaattt
     6421 tccatgatgc ttgtaataac ggataattcc aaggtgtaat ttgtgtaact acacctactg
     6481 gttctttaac aattttgctt tctgtatctg gaattggtga atcaatcatt tcgccaccgt
     6541 ctttatctgc taatccagca aaatacataa acacattatg aatatcatcc atatctgcat
     6601 atgattcttc taacgttttt ccagtatcta atgtttctaa tcgtgctaac gcttcgcgat
     6661 gttctttaat cttgtccgcg atggcacgta cttttttacc tcttgtttca gcagtttctt
     6721 gcgaccattc accagactca aacgcacgtc ttgcagctaa gattgcacgt tctgcatcct
     6781 cttttgtccc ttcagaaacc gtaaatatca cttcttgatt gtaaggattg ataatatctc
     6841 ttgtattttt attcgcgctt tcaacccact caccatcaat atattgacgc tgagataaat
     6901 gttttaaaag ttccattcaa caacctccgt taagtttgtt aaatttttat ttaacgaatt
     6961 ttacaaactt atgttagcat agaaatgaaa aatgtgtcaa tttacaaaac aactcaagaa
     7021 ttaagttttg taagttcgtt agatttttat ttaacgaaac atttaacttt aatgctttaa
     7081 atatttttaa tgtctttttt tagaaaaggt tttataatgt attagtattt attttaacgt
     7141 gaccaaaatg atgtacggag gtgcaatgat gactcgttct cagaacaaac agcaacaatt
     7201 agaacaagca aaagatattg taattaattc aattggacag acaatggatt tgtatggcac
     7261 taatagaagt gtaggaaact tatatggcac aatggtgttt gaaggcagta tgacgcttga
     7321 tgaaatgcgt catcaattgc aaatgagcaa accaagtatg agtgctggcg ttaagaaatt
     7381 gcaggaattt gatattgtta aacaacaatt tacgagagga agccgcaaac aacattttat
     7441 cgccgagaaa gactttttca tctttttccg aaactttttc actaaaaaat tccagcgtga
     7501 aattgacatc aatgtcgaag cagtaaaaga tgctcaagct attatcaatt cattacttga
     7561 aagcagtgat ttaactgaag ctgaaacaca agaggcacaa aaaattaaat ctcagcttga
     7621 tcatacacat gtttattacg aatggttaga acagttaacc gaagcgattg aaagcggtga
     7681 aattttcaaa tatttcccaa ttccagaaca gccttctgat tctgaaaatt aaaattgact
     7741 caaacaacga caatgatgat tatgcatgat aaaaacaatg caataggtcc acgaaataaa
     7801 cggtaatttc tatctcaata aatagaaatt accgtttttg ttttatatga caatcaatta
     7861 tggaggtatt ttctagggct ttcttagatt cgttacattg cttcgccaat atatttcaat
     7921 tagctattct cgccctcacg tactctatca ataatatttt gtatttgttc acgtttttca
     7981 tattcatttt ctacaaaata aactaacggt tcatctttga tagtaaccca aggtacaagt
     8041 acatcaaaat ctaattcttt aaccatctct aaactttgaa tagatttttc tctatcactt
     8101 gaacctaata taactgtacg ccatctcttc ccttcaaaac atataaaatc tccagtaaat
     8161 aagtaacggt gatgaccatc atcccataaa aatagtgtcg tccctggtgt atgtccaggc
     8221 gcgggaataa cttctaaatc cttatccaac attacacgat tactaaattg tttttgaaca
     8281 gaaatcttat gtttcagtgc agccacatca ttttcatgta taaagtaagg ggcttcaact
     8341 tgattagaac ctcctataga ttcatgatca tgattcatca atacttttga aacaccacct
     8401 aatgatgcaa tgtcttgttg ggagtcacct aagtgtccgg aatgatagat aacaatatta
     8461 cctgtttcac gttttaaaat aaaagattta aatagaaatc gattatcaaa aggcagttcc
     8521 gaagtaggtg tcgcatataa gttttttgtg atggattcta aactgtcatg taatttggac
     8581 tgtttatttt taaattgatt agtcatttta tttcatcctt cgattaattt gaattgtaca
     8641 tacgcagtac cctttattta atttaataat caatgagagt ctttcaaact tcgatttaga
     8701 atatgcttca tacaataaat aagtacttgg agcactaaaa aattaaatct tttagtacaa
     8761 cattaatgaa aacctaaaag ttcatcctac aatgctacta aaaaaagggg aatggaacag
     8821 aaatgatatt ttcacaaaat tcatttcgtc gtcccagacc cgctttgaat tataaattat
     8881 cgtcttgttc ttcttctgat acttgaacga ttcgcaatga acgacgttca acttctttta
     8941 atttttcagc acgcgtttca agtttaattc tatcgcgccc taaaatgatt aaaaatgata
     9001 tcatcatgaa aataaaaata acaattaatg gcacacttgc cagtattgaa gcagttttca
     9061 atacttctaa tgcacgttca ccaccaacta gcatcaatga aaatggcaat aagcacaatg
     9121 caaatgccca gaataaacga ttggcacgta atggttcgcc taccactttt ttctgagatg
     9181 ctgccgctaa aatatatgaa cccgaatcaa atgttgttgc taagaataag aaagcagata
     9241 ctaagaatag tacaatcatc aatgatggga atggtaaatg atgcaccact tcaataatgg
     9301 ttgcctctgt accatgtgta tttaaatatt gtgttacatt aaactgtcca gaaatttgta
     9361 aatacacagc atagttacca aaaataccaa agaataatac gcatccaagc gttccataaa
     9421 taattgttcc tagcacgact tctttaaggc gtcgaccttt tgaaattcta gcgataaata
     9481 aaccgataaa tggcgcatat actaaccacc atgaccagta gaatattgtc cagtcttgtg
     9541 ggaaattcgt ttcttttcga cctttaatac caccgaatgg ttctaaccat gttgccatat
     9601 gaaagaaatc tctcaacata tttccgaacc ctgtcactgt cgtttccata ataaaaacag
     9661 tcggtccaat aataaatata aaggctaaaa gtacaaagga tagccaaacg ttgatatcac
     9721 ttaacttttg aatacctttt ttcaatcctg tatatgaact aatggcaaat ataaccgtga
     9781 ttgttaataa aatggccgaa cgtaaaatca tatttttacc atctaaacca gttaatcttt
     9841 ctatgcctgc agaaattaat ggcacaccta acgctagtga tgttgccgca ccacctagca
     9901 atccaaagat aaataagata tctacaactt tacctacaaa tttatctgtt tgacctttta
     9961 aaatcggacg acaagcttga ctaattttat acaccggttg ttttttaaca aatactaaat
    10021 aaccaattgg taatgctggt agaacataaa tagcccaagc aattggcccc cagtggaaca
    10081 taccatattg cgtcgcatat tggagtgctt catcactcat acttttcgcg ccatttggtg
    10141 gaacttgata gtaaaaagcc cattcaataa cgccccagta taaaatatca gagcctatgc
    10201 ctgcacaaaa cagcattgcc gcccatgtaa atgtattaaa ttctggttta tcacttgctt
    10261 taccaagtgt gacattacca tatttaccaa atgcgatata cattacaaag caaaaaatcg
    10321 ccagccccat aaataaatat atcgaaccaa ttgaatcaga aatggcacta ttaataccag
    10381 tgatgatatc ttcacttgct tttggaaaag ccatcatagg tataactgca aaaagaagta
    10441 cagctactgt ccctataaag gtcgtccagt ccataacttt ctcttttttc aattgtgctc
    10501 cccctaatta ttaattttat gaatcctgtt tcgatttatc tcaaaatgta ataattatat
    10561 tgattcacaa aattgacaat aactaacatt taataataat gcaaatttca tacaatttga
    10621 aacttggcaa ttattgaata tttatataat ttttcccgta ataaacaaaa ccttaatagc
    10681 gctaaaataa cagtgttaag ttacgattta acgaatttaa caaattttac tagaatggca
    10741 tttaagaata tttatacgtt attaacgaat atttatttat tgtaaaacgc taccaaaaag
    10801 ttagacttcc ttcccactaa aataccactt ttctctttca acttttttaa aaaacggata
    10861 tgcaactttt agtattggta tcaaaatgat tgttaggtca tattctatca atatattttt
    10921 ataaagaatt gcttttatta actttcaatt atgtacctaa cctaaaaaga agccaaggca
    10981 acgaatgtta ccttgacttc taatacatat tcaactaact atatattcaa tcatacgcgc
    11041 atgcgagagt gattgttgta catctataat gcgttgattt aaagaacctt tatatggtaa
    11101 atcaggtttg aataagtgtt gtataaatag accatctact aaaacgtcaa tgtatgataa
    11161 taactctcga cgttctgtac aatcatttgc taaatattca tataaaaatc cagtccatac
    11221 ccaaattgtc tttgtatttc caaaacgtgc tcgaaatgct ttgacaagat ttaatgtaat
    11281 atccaaatta caaaatggtt cgccacctaa tagacttagc ccagatatat aatcatgatc
    11341 gcaatcatct aatatttctg ctaatatttc atcagtgtat ttctcgccat atctgaactt
    11401 ttgtgaggct ttgttataac atccaacaca attaaatgga catcctgata cataaacact
    11461 gcatcttact ccttcaccgt caacaaagct atttgattct attttagcaa tataaccttg
    11521 tccttgttta atgtctaaaa gtatcattct ttaggcgctt tcatatgttt tactcgtgcg
    11581 caaatttctt tatgacggcc tttaattact ggacgttgaa ctggattgcc taggtaacca
    11641 catgttcgtt taacgacatc aactgtttta ggattatcat tgccacagtt cgggcattta
    11701 aatccttttt cagttgcttc aaaatctcca tcgtaatcac attcataaca atgatcaatc
    11761 ggaatatttg tacctaagta accaactttg tcataagagt agtcccatac cgcttctagt
    11821 gctttcaaat tgtgttgcaa tttcggatac tcacaatagt gaatgaaacc accactcgca
    11881 taataaggat aatctttttc aaaatctaac ttttcaaaag gtgtaacatc tttacgtaca
    11941 tcataatgga aagagttttg ataatatcct ttatctgtaa tgtctttaat atctccaaat
    12001 ctctcttggt ctaaacgaca aaaacgatcc gttagcgatt cactcggcgt actgtaaata
    12061 ctgaaccaaa tgtcatataa ttctgtccat ttcgtttgat aacgtttcat ttctttaaga
    12121 atttcaagcg taaatgcttt tgcttcttga gatgtttccc agtctggacc atagaaaaca
    12181 gtagctgttt catacaaccc tatatagccc attgaaatcg ttgcacgttt atttttaaat
    12241 aactcagcaa catcatctgt ttcttttaat ttatagttaa atgcgccact tttatataaa
    12301 atcggtgcgt tattcggtac agcatctttc aaacgattta tacgataaag taatgcatca
    12361 tgtaacacat cgatacgttc ataaaagatt tcccagaatt tcgtcatatt accggcagat
    12421 tctaatgcca ttctaggtaa attaagtgta acaacaccaa gattacaacg accattattt
    12481 tcaaaatgac cttccgcatc tttccaactt ggtaaaaatg aacgacaacc cattggcgct
    12541 ttgaaatcac ctaatatttc tacgagtttg tcataattta aaatatctgg atacatacgt
    12601 ttcgttgaac actttaatgc tagttgttta atgtcatagt tcggatcttg cggactaaag
    12661 ttggttcctt ttttaattga aaatacaagt ttcgggaaaa tcgctgtcgt gcggtctttt
    12721 cctaagcctt tgatacgagt atttaagata gcttgttgaa ttttgcgact taaatgatct
    12781 gtacctaagc cgaatcctaa tgttacaaaa ggtgtctgtc cattagatgt atataaggta
    12841 ttaatttcat attctaaact ttcaatcgca tcattgatgt ctttagtgac ttgttgatca
    12901 acataacgat caatttcaga ttctttgaca aattgctttg cgatattcct atgttgttct
    12961 tcattatgtc gtgcatatgt actaagtaat tcgtcaacgc ggtcaaccgt acagccacca
    13021 tattgactgc tagaaacatt ggctataatt tgtacaagct gcgctgatgc agtttgtatt
    13081 gattttggtg aagttacatt cgcgttgcct atttcaaatc cattatgtag catattttta
    13141 gcatctatta aacaacagtt agttaacggt tggaatggat gataatctaa gtcgtgaaaa
    13201 tgtatgtccc ctctttgatg tgcatctgca acgtgctttg gtaataaatg atttaatgcg
    13261 taagatttag aaacgatacc tgctgttaaa tctctcattg ttgaaaatgt atcactatct
    13321 ttgttagcat tttcgttaac aatagcagga tctttagaaa tcaaacccgt taatgcttct
    13381 tcgatttgat tcattttacc ccttcttcct atatattgtg tctactcatt acgttatacc
    13441 actatatata gtattttcaa atttttatta gtctgttaac taaaacgtca caaagttatt
    13501 aacatttagt gaaattcata tttctacaca attatttcat taatttttaa aattgtttaa
    13561 agattcgtcc gtcatttcga catgtttatg caactattat ttcattttgc aacgtgacca
    13621 tagtttctat tttttcatac ttaaatcgtc acaatgccca tcaacattgc aattactaac
    13681 ataacgatag cgaatcccca aatccaaaag aatgcatact taatatacgt gcccatgttt
    13741 gcctctgcta aaccaattgc caaccataag gctggtgaaa atgggctgac aaatgtacct
    13801 ataatattcc ctatgaccat tgaataagct gttgatacag acggtacacc aaattgccct
    13861 gctgtttgtt cgacaatcgg taacacagca aaataataag cgtctgtgct agttagtaaa
    13921 tctaatggta cgccaagtaa acctacaata atatgcaagt atggtcctac ttctgcagga
    13981 atcactttga ttaaattggt cgcaatcgct ttaagcatac cggtttcatt tagtacacct
    14041 aaaaacatac ctgctgcaat aatcactgca gccatcatta atgcattcgg cgcatgcgct
    14101 cttaatcgtt ccatttgttc atccactgat ttaaaattaa taacaagtgc caacgaaaca
    14161 cctatcatga atgcaaattc aggtggcgca atatttatta acatcgatag aataacagct
    14221 aaagttaaag ctgtattcac ccattttatc catgattttg ttcttgcacg tccttttaca
    14281 ggaaacctta catcttgatc tcgttcatat acttcaacta atttatgtac atctatatct
    14341 tgtgtttgcg gtaattcatt tctctctatt gcttttttga tacgtttctg ttctttaaat
    14401 ccaagatata ccgcaaacaa cataacaaga atgaaaccta ttatttgaat aggtattaat
    14461 ccataccata attcattgac acttttggct tttaacactg cagctacacg agccattgga
    14521 cctccccaag gtaccatgtt cataatcgcc gcgcttaatg ctaatagtaa aatcaataaa
    14581 tatttattca tatttaacgc tttatataaa ggtaataatg caggaataga aagcaaaaat
    14641 gttaccgcac cggccccatc taattgggct attgtgccaa ttaaagctgt cattgcacag
    14701 acaatgacga cattgcctcg tgtcattaat attaagcgtt tgacaagcgg cttgaataaa
    14761 ccactatcgt tcatgatgcc aaagaaaata atggcaaaga taaacataat aacaacgttg
    14821 atgacttgat ctaacccttt agcaaaaaat ccaaccaaat ctgtcacact atatcctaaa
    14881 atcattgccc ctaagcaagg tatgattgtc atacctacaa ctggatttat ctttttggca
    14941 atgagtaaac ctacaattga aataataata atgagcccca ttactgttaa ccacatatta
    15001 tcactattca tattattttc tccctccact tttaatacaa tttacataat ccccctcttt
    15061 aacttaaata ttataattaa tataattttt tatatttaag ttatctcatt ttatcaaaaa
    15121 aatctttttt gcgtaactat caatagtgaa gtttgatttt cctacttgat aacatggcat
    15181 gactaatcct tgtatatcct gtcctgtatt tcattttgat ctatcatttt tattattgct
    15241 ttctattgca caaaaaatct ggctaaaggt cgctatgata gtaacgactt caaccagatt
    15301 tcaatttatc cacctatttt attaagtttg ttctacgtat ctttttctct attgtaatat
    15361 tcatatttaa aacgcatatc attatcgctt aaatccttca ttattagact attcacaaag
    15421 ccgcccatgt cttaatttca atgattaagc gttaaagtta aaataagaag acttacctta
    15481 gacggttaga cggtccgcta ttattatctt acatccaacc acgctaaaaa ttgagcttgt
    15541 ttgtcatgat ttaaatattc ttgtgacaca atttgtgaca gtaactgttg cttttcgtta
    15601 tatgttatat caagtacttt tattttctgt cggcaagtat ataaaaagtc gatatacgaa
    15661 ctgtatgatg gtggatataa cgcctcaagc tctgccataa ttgattttgt caacttagga
    15721 ctcgcaccat cagttgatac actgatagtt agcttgtcgc ggtgtagtgc acttggaaat
    15781 acaacattgc catttgatgc atctccaaca ttattaaaaa gggcatgctc aggtaaggct
    15841 ttttttaccg cttcattgac acgtggctca ttggttgctg caataactag atacgcgtct
    15901 acaatatcgc ttggttcaaa ttctttttct ttccatatga cgacaccgtt atctaccata
    15961 ttttgaagtt tttcagtgat tgtcggactg atgaccgtca tatgttcaac gtattgattt
    16021 aatgtttgtg cccgacgact tgcaacgacg cctccaccaa ctatgacgac atttttattt
    16081 gttaaatcta tcattaatgg catattcatg ttgagcaaca tcctttatat ctttttacat
    16141 cattacacct taaaatattt aacaccaatc gctaataaac atcacgttga tagcgttgtt
    16201 gttgtttcat ttgtcgcaat aataactctg cttcttcttg agaaatatga cgttctttta
    16261 ccaatacatc tttaatggct tgatggacat ctttcgccat acatttttca tcgccacaaa
    16321 tatagattgc tgcgccttgc tcaatccatt cattgaaacg tttactttct tccattatac
    16381 gatgctgtac atattctttg tgttcttggt ctcttgaaaa tgctaaatct acgcgtgtta
    16441 agtttccatt ttcaagccat tcttctattt cttcttcata taaaaagtca gaactacggt
    16501 gttgatcacc aaagaacaac catgtttttc cagtcatacc aagttcttca cgttcttgta
    16561 aatatgctct aaaaggagca attccagtac ctggtccaat cataataacc ggtatatctt
    16621 gcttcatcgg aaatttgaag ttcggatttt tcttcaaata aattggtact atatcgcctg
    16681 gtttaattcg ctcagcaaaa tgaaccgagc atacaccttt tctctcacgt ccatgtgctt
    16741 gataacgaac cgtaccaacg gtaatatgca cttcatctgg cgttgccata aaactactag
    16801 aaatcgaata ctctcttggt ggtaattttc taaggatttg atacatattc tcaggttgta
    16861 attctatagt tggaaaatct gttattaaat ctataaagtc ccgatttata acatattcac
    16921 gcgcccatga ctcatcttga atacgttcag ataattcttc attgtcaaaa tagatatctg
    16981 catttttcaa taatggcaat gttaatttag taaattcaaa atgtgatgtt agtgcttcaa
    17041 caataggaac tgtatcacca tgatcattaa ttggcaccgg agattgcgga tcccaaccta
    17101 acatgaatat tagtttttca accaattcag ggtcgttttg cggtaatgct actatacaat
    17161 ctcctggttc atatgattca ctaaaatcat caagtaaaaa ttctatatgt cgtgtttctt
    17221 tatttgaatc ggtaccattt aaattgatat tcgctaatac ttctgcttgg tatggatttg
    17281 atttagaata tttcttttct ttggcagact taattgattc acttatcacc gattcacttt
    17341 gaataccttc tgatgtggta tcaataatat taatgatgtc tgccatccac ttttctgcgt
    17401 cttcttcata atcaatatca caatctacac gcttacatat acgctcagcg cctagatttt
    17461 ctagtaaaac atctacatct ttaccggctt gacagaaaaa ttcatatgtt tgatcaccta
    17521 aagctagtac tgaatatctc acatgattta aattaggtgc gttatcgtct tctaaaaatt
    17581 caaagaaatc ccatgcatta tcaggcggtt ctccttcacc atgagtagac gtaataataa
    17641 ataaatcttc taactgcgcg atgtttgtcg tatcatattc atccattgac atcaaaacaa
    17701 cttgatgtcc gatatcactt aaacgttctg aaaaaatttc agctaaacgc atggcattac
    17761 ctgattcaga accatatagc actgtaacat gccgctgatt agcttcgata tgtggctctt
    17821 tattttgtaa catatacgtt gttgaagttg cttgttcttc tgatatttgt gattgttgcc
    17881 acacttctgt ctctaattgt tgttgattac tatcacttgt cgtatcgtta ctattagcta
    17941 gtaaaaaacc actaagccac tgttgttggc tctctgttaa tgtttgcagc agattgttaa
    18001 tctctgtgac ttgtttttct gtaaatggac tattagatgt atttaatttc aaatgttttc
    18061 acctcaataa acttgaaata gatgattgac tcttttttta tcgatagttt agtcaatttt
    18121 ttaatatgta tggtataaat taaaacaaac ttgagttgtg tctgatagtt atacttgcaa
    18181 atatttacaa ccttcatgtt gcttgaatga cattatcagt aattcagaca aaactttgat
    18241 tatgtcgtgt cagttattaa tgcatacagt aatgcatcgt tgttattttt tatttattta
    18301 ctatttatta tgtaccaaat cagcctttca gtaaaatagg tcaaattact gattttctaa
    18361 atataaaatg cctcctaata acatactatt agtacatcat taagaggctc ttgtgttatt
    18421 tgcatactaa gcgctcaaat ttaaatttaa gatgaagatt cttgcaataa tatttctata
    18481 tttgtcgata tatccaatgg atcttccatt ggcaagaatc gatttacaac atttccttgt
    18541 cgatcgatta taaattttgt gaaattccat tttattgggg acccaaagat tcctggttgt
    18601 tcattcttta aatgcgtaaa taacggatgt tcatcgttcc cgttcacaga tattttagct
    18661 aacactggaa atgttacacc aaatttctca cgactaattt tcaagatttc ttcattagaa
    18721 cctggttgtc gattgtcaaa attattattc ggaaaactca acactacaaa cccacgatcc
    18781 ttatattttt gaaataaagt ctctagtttt ttcaattgtt cgctatatat acattctgtt
    18841 gcagtattaa caactaaaat cactttacct ctaaatgctt ctaatttata agttaagcct
    18901 ttataatcac ttacttcgat atcatacaca tttctattat tcataagaca cccctacaca
    18961 gcctttttta tattgaatat gttcttttta gaatgttctg ataaaataag tgcgcgttta
    19021 caccgtgaac acacattata tagcgtgata catttttcga gcacacgata aataatgttc
    19081 gagtttatgt tgttgctcaa cctatccgat ttaccgtctt ttttcacctg tcttacttta
    19141 ttaaaataat cacattgtat agctgtatta gtccagctac aatatcttcc gttataaccc
    19201 ctcattataa tcatccttat tttctatttt taaaaagaca attagaccgc tctttaaact
    19261 atagattaat acttaagtta aactcataca tactgatacc atacgttaga tctaacaatt
    19321 taaaattcgt tataactatg gattaaagag ctgcccaact catataatcc ttaaaaactt
    19381 cacatgtgat tgtttattaa gccctccttt atcttattaa atatccttat aaccctttta
    19441 aaattaaact gacacactca tacattgtta cacgcgacga taaacgtcat attattgcat
    19501 atcattaaaa caagtcactt tgtatcgtgt gcgaccgaat acatattaaa tcatcaataa
    19561 tgattattca attattaaaa ataaaaacga acattctata tagagtttct acttattatt
    19621 ttttcagcat tgtctacatt acttaacatt cgtgtttgta agtaatattg accgccaata
    19681 tttagacact ttataagtat gccattcatc atttttaatc atattatttt tcttattgat
    19741 atttctattc atatatttct gattgtatat ttagatataa taatttgtta ttggaaaata
    19801 tatgaaacaa atttgactca tctttttcaa tatctaaatg acacgatgca tgatttttta
    19861 attttgtaat taacttcctt ttaaattaga ctgaattgtg acaattttca aacattttaa
    19921 tgtgtaaatc attggcaaaa tatacgaatt gcatttaaat cgtcaattta catatatttt
    19981 tgcgtagtaa tcaatcgtta tcattgttat ttatcgttac attatttcga gtatcagtat
    20041 gtatttcggg cttcgtttga taacgacatt tctttgtgac atcgcttcat cagtgtaaca
    20101 acaaatacaa tgatttcgtg atgttagtta cccattttat gtgttgcata aaatatgttg
    20161 ttataaaaca ttttaaatca ttttatataa acaatctata tatttttggc atttccaaaa
    20221 tatcacttgt tatattaaaa accgacaaga cattttatct tatcggttga aatttgttat
    20281 tgttatttgt aatgttttta ggtttctttt taatataata tatttcagtg aaaatacatg
    20341 attgattgtg atttcactga aacatggtta attgcgttgt tgatgaataa ctttagcata
    20401 aatataggaa gttattttgt acatcgccat atatagaaac gaaattataa tgacagctag
    20461 tacgtaactt gttaaaaata tatgatggtt attaatacct atcatattta gtaacgtata
    20521 tacaatgtta ctagaaatta atgtgtgaat cagtgctact gttattggta ttgcgaacaa
    20581 gaaagtcatt tgatttcgtg ttatctttgc tattcttcca ttatctaaac caagtttttt
    20641 cataatgtca taattgtgct gatttgcata ggcttctgag acttgtttat aatacatcat
    20701 tagaaatatt ccaatgacaa gtacaattga tactacgcca cctacaaata ttagtccacc
    20761 tgtcaaacga tcccaaacca ttagcatttc tgatttagaa tttattgacg catcgtattt
    20821 tttcgatagc ttttgtattt gtgatgttgt taatttcgtc tttccaaata cattaaagtt
    20881 tatcgatatt gtcgaattct tttgttcatg tgcataaaat ccttttactt tttgtaattg
    20941 attgttatct ttaacaatta aaaccatact atcttgaatc atcatcaaat ttaacgcatg
    21001 tgcgtcaagt tgtttcactt taaagacttc attattaaga ccgaccttag actgcttttt
    21061 aaaaataggt acattcgtaa tcatacctat actttgattg tctaagtgta atttgttttg
    21121 gtgatatcga ttataatctt gtgcagtaaa agctgtaatc attactgatt gtcttgaaaa
    21181 tatcgcgcct tcattaccaa tgaagtctat tggaatatca tcactagcct gttttaactt
    21241 gtaataagca cgattctttt ctaaagaagc tctaaataat gtcgtttcat atactttgaa
    21301 gtcttttgca ttaaccgaac cctgtatatc acgtttaata ttctcaattg tttgttgacg
    21361 ttctatttgt gacttagaat tgtcgctaaa tgacaaatca taatcattcg taatcaattt
    21421 cgtaatggta tgattcatat cacgataggt tgttaatgtc attgtgagcg ttacaatcaa
    21481 aaatgtacac agcaaagtga ttgttgcaag actgactgca ttcattttaa gacgtacacg
    21541 catcccaact accacaaaaa aataccgtgg atgatagtaa acttttgata cctgctgcaa
    21601 tatactaata attatttcac ttatacctac aaaaaatgca taagtaccga taataactaa
    21661 tcctatgaca atccatattt taaaaaaggc accaaacgtc gtatcttgtt gtaatgcaat
    21721 aaagtaacct aaatatagtg ctgcgcttcc tataacaatt aaaacgtaac gcaaccatcg
    21781 tgatatgaca ctagaatcgg aacgatgttg ataagttatc ggccgttgaa aattaatact
    21841 aaacaagttg aatataagta gcatgcccat taatacagca atgataatca aagtaataaa
    21901 catcgctaca gagtcaaatg gataatacct taacgtcgca acttcactgc ccattatttt
    21961 ctgtataaac aagaaaaata tcgcaccaag taagtatccg ccggcaatac taacgactga
    22021 aattatcaca aattgtaaga tagtttccat cactacaatt aaacgcatac ttttcttggt
    22081 catgcccaat gtcataaaaa tgctaaactc ttttcgtctt tgtgacatca taaagtgatt
    22141 tgcatagaaa ataaaaataa aagttaaaag cgccataaaa aaattagcta tgataataaa
    22201 tggtactagg aagtcattct tctgttttat gtagctattt aacccaattg acacaagcgt
    22261 atattcaatc atgaatagta cacttaccgc taaaatgaat ggaatgatta caagtcgctg
    22321 cgtaataaat tgacgtcgca ctatgtgcca taacaatctt aacttcatat attgacactt
    22381 cctccattca caagtgctaa gctatctgtt attcgttgtt gaaaagctaa ttgactttct
    22441 tcaccacgat atatttcatg atatagacgc ccatctttaa taaaaatgac tcgctctgca
    22501 taagacgcat cgatatttga atgagttacc attaaaattg tctgttccaa ttgatgaatc
    22561 tcttgaaata acatcatcaa tgcctttgat gtttttgaat caagtgcacc tgtaggttca
    22621 tcggctagta aaatcgtcgg cttagtaact aatgcacgtg caatggcaat gcgttgcttc
    22681 tgacccccag agatttcaga aggatacttg tttaagaatc cctctaaatg taattgtact
    22741 gccaactgat gtaccctttg ttctatatct tttcgtttag caccagctaa aattaatggc
    22801 atcattatat tttctttatt cgtcattgtt ggtaaaagat taaaatcttg aaaaacaaaa
    22861 cctaccattt gttgacgata caatgcttta cttttatttt tcatattatt taaatgtgcg
    22921 ccatccacaa taatgtcacc ttcagttagt ccatcaaaag aagcaattaa atttagtagt
    22981 gtagacttcc ctgacccaga ctcacccata attgcaacaa attcaccagc tccaactgat
    23041 aaattcattt gattaagtgc tgtcgtagca ttcaaacctt taccataaac cttttttaca
    23101 tgtttcactt ctagcaacat ggcatgcacc tcatttcttt gttagttcta tattaaaaca
    23161 aaaaaatact aataacattt aaattagtaa aaaaatgtta ctagtatact tacaatattg
    23221 aaagttttta ttcatctgga aattgaatcg taaatgttgt accctcattt tgtttagata
    23281 cgactgaaac aggatggttt gtgtgtgttg aaatttgttt tacgataaat aaaccaatcc
    23341 cacttgagtt actttggcgc tggccattat aacctgaata gcccttatca aatattttag
    23401 gcaagtccgc ttcactaata ccgataccat tatcttttac gtgtaattga ttggattgct
    23461 catcaaattc aatccatata tctttacctc tcgcatactt aagtgcatta tttattaatt
    23521 gttctatcat taaagaggtc catctaacgt cagttaatac ttcgtgatga caaggttcat
    23581 aatggatttt tgttttttga tcaataaact gtattgaata tttcataata attgggcgaa
    23641 tgatattatt aatcgaaatt ttagtgacag aaatatcaga agtttcattt aataacttta
    23701 aataactaag tgctaaactt gtatagttat caatttgaat aacctcttga cgaacacgat
    23761 taacaacatt aggctcatct ctttcaagta acagttgtgc tgcagtaatg ggtgttttca
    23821 tttgatgtac ccatgttaaa aagtagctct ctacatcatt tttatattca atttgttcat
    23881 ttttaagctg atacaacgca ttttctaagt tttcaatttg ttgttgttgg cttatagttt
    23941 ttacaaaact taaatattta atacctatga atatgagtaa taataataaa ataacgccaa
    24001 gtgctagtaa atatgcttct aatggcaaat gatacaggta aaacattaag ccaaacaaag
    24061 caaaaataac tatgactatt gctatttcct gagtaatact tttaagaaag gtcattgcat
    24121 tcaccctata ctttatatcc gacatttttc tttgtaatga taaaatcatt aacgccaata
    24181 gtatttaatt ttttacgcag gcgcgtcatg ttaacagcta atgtgttatc atctatgaag
    24241 ttttctgatt cccaacattt ttcaattaaa gcagttctac ttacatattt atcttcattt
    24301 tgaaataata actttaatat ttgtaattca gtcaaagata gctgtatgtt ttgttcttga
    24361 tacacgactt ttgcttcatc taatattaat gtacaacctt taaccgtcaa tgaatcatta
    24421 gctactgaca agtcataagt tcgtctcaat aatgcttgaa ttttggcaat cgttaatgac
    24481 aagttaaatg gcttttcgat aaaatcatct ccccccattt gtattgccat aatttggtcc
    24541 atattatcaa tacgggaact aataaatata attggcacat tagatgtttt tcggatttct
    24601 tgacaccaat gaaaaccatt taacgttggc aaattaatat ctagcaatac aagctgaggt
    24661 tgattttggt taaagataga cagtatatca tcaaattgtt caacgacaat aacaccgtaa
    24721 ttccattttt taagttcaga tgctaaactc tctgctataa caaaatcatc ttcaacaatt
    24781 aatattttca tctataatct tcttccttca attgaattat cctttgttat ctttttgttc
    24841 tttatcctgc tgcaatgacg gctcttgatt tgaactttcc gtgtcatttg tattttgatt
    24901 tagtgtattt gctttttctt tctcgagttg ctttttcttc gtacgtttac gctcttctaa
    24961 aatagacaac actataaaga atatgattaa taatggagat aacattacta gtatcatttt
    25021 tccacctcaa attatattta attctatatt ttcacacgct tctattctaa cgctttctat
    25081 aacaaattgt catatatatt cattttttgt tattctaaat cactctgttt acatattttt
    25141 aactaaaact acataattgc tcaaaattcc tcttttacac tgaagacata gtcatatact
    25201 caggaggatg tcttatgtta tcagttaaaa aagccacaaa atttgcagtt gctaacctta
    25261 ttatcgtgtc aacggttttt agttctgtca cacctgcaat tgcgcaatcc gataaaagtt
    25321 ctaaagatga aagtttcgcg gttggtaata ctaaaaatcc aaaaaatgtt atctttttag
    25381 ttggcgatgg catgggtcca tcatttaaca cagcttatcg ttattataaa aatgatccgc
    25441 atgctaaaga aatgacccct actgcttttg attcatattt aaaaggcaca aatcgtacat
    25501 attcaaatga cccaaaacag aatattactg attctgcagc tggcggaaca gcctttagtt
    25561 ctggtcataa gacatataat ggtgccatcg gtgttgacag taacaagcag aaagtaaaga
    25621 cggtgcttga acgtgcaaaa gaaaaaggta agtcgactgg tcttgtttca acagctgaat
    25681 taacagatgc aacaccagca gcatatgctg cacacgtgac ttctagagat gataaaaacg
    25741 aaatcgctaa acaattttat aaagataaaa tcaacggaaa gcataaagtt gatgttttat
    25801 taggcggcgg cgctaaatat ttcggtaaaa gtaatggcaa tttagataaa aaattcaaaa
    25861 aagatggtta cgaccttgcc acgaacagta aggagttatc aaaatctgat aaagataaag
    25921 ttcttggttt gtttgcagat aaaaatatgc cacttgcaat agatgcttct aaagacgaac
    25981 catcattagc tgatatgcaa caaagtgcac ttagtaagct tgagcgcaat aaaaaaggat
    26041 tctttttaat ggtcgaaggt gcttctatag ataaatcagc gcattccaac gatattacag
    26101 gcgttatgtc tgaaatggaa ggttttgaaa aagcattcga tgacgccatt caatatgcta
    26161 agaaacataa agatacactt gtcgttgcaa cagcagatca ctctactggt ggtctaacaa
    26221 ttggtaaaga taaaggatac gaatggaatc ctcaaccgat taaatctatg aaacactctg
    26281 gatcatatat gacagaaaaa atggctaaag gtgaagaccc tgaaaaagtt attaatgaag
    26341 gttatggatt tgaattttca caagatgata tgaaaaaggt taaaaaagag aataaaaagc
    26401 taaaaagact acttaaaaaa gaaaaagacg aaaaatccgc agaagtagaa acgcaaacaa
    26461 aagccctaca acacgctatt cagaagccag taaatgataa atcttataca gggtggactt
    26521 ccgatggaca tacaggcgag gatgttaata cctacgctta cggacctaga tcagaatcat
    26581 ttagtggtaa tatcgaaaat acagaaagcg caaaaataat atttgatata ttcaagtaaa
    26641 gcataaaaaa attcccgagc actataacaa ggtgttcggg aattttttat ggtttaattt
    26701 agtactcaat gttaggtgct tggttaacac gttcttggta ttttttaaat gcgaagtata
    26761 aaccgccaac taatgttaaa ataactgcta attttttcat cattcaaact cctttctaag
    26821 atgtgttgaa actgagttca attaaattat atgtttttat tatacacttt ttgacatatt
    26881 ttttaaattt aagaatgcga agatttttaa catttctgat gctagctttc tttttcatct
    26941 ttcaaatgct taataaaatc aaagccaggt cgccctttag ccaatgcttc catcacatca
    27001 taccaatccg aaccgacttg ttgatatata ttttgcaata attcatcttg cgccttgttc
    27061 aattcagcaa tgagttgatc gcctttatcc gtcgaatata attttttgac acgtcgatca
    27121 gtctctaaaa ctttttcaat aatgagacct tgctctttta atttttgaag tgttgcatgt
    27181 gatccttgtt tagaaatttc taatatttct agtaatgatt taatagtaat accaggtaat
    27241 ttattgataa aaaacaaaaa acgatgatgc tgacgactca taccatactt ctctataatt
    27301 tcatcagcgg tagtaataaa ggttttatat gcaaaataaa acaacatatg ctcataatcg
    27361 tttttattgg tcgacatgat gatgactcct ttcaaagcgt taacctcatt ataataaaat
    27421 ttataatgta agtcatacaa atgatgataa aactataagc taaatctaag tttcacgaga
    27481 aacatttttg tgtatcagca aattgcgcca atcatattaa tacaaaaata atttaaacac
    27541 ttagaaatgt tagtcgtgcg taaatagttt gtatctctca ctctgtgtat ttaatgcagc
    27601 cacatatttt ccaagaaata attaatcatt caccatccat gttatagcac gcttaatcgc
    27661 ttgatcccaa tatgcataat catgatctcc tggtccatct tcaaattgat aaggaacatt
    27721 tatgcgtgat aaataatcga taaaatctaa gttgtcttga tataaaaagt cttgtttacc
    27781 acacataatg agcaattttg gaatttgttt atcttcagct acagctttgt ctagcaagta
    27841 atacggatca tgttcagttc ctttaacact tgaaagattg ccaattatgg cctcttttga
    27901 aaaatcattc cactctagat ccattaaatt ttgcgcttca aacacagcag ataatggtac
    27961 agctttggca aatttatccc cttgtgttaa tgcaaattta attgtgccat atcctcccat
    28021 agagtgacct gctataaaat tgtcatcacg ctttttggaa agtggaaata tttgatgaac
    28081 ataatcatac acttccaaaa tataatcata atagctatga ccatatgcca tgttagcata
    28141 tgcgctatga tccacattgg gcataatcac agctaatttg tgttcattcg catacctttc
    28201 tatgcttgta tatctcatat atgtcgtttc atcacttgat aatccatgta acaacattaa
    28261 agtttttaat ggtttaacag ttgtatcgct attaaagaag ctttgatctt ccggtaaaat
    28321 gactgtcaaa ttttgatgca taccaattgt tggtgaatga tagtttaatg aaatataagc
    28381 catacgtcat gacccctttc taattctact ttatcaacat tttacgctta atcaattcac
    28441 tttaaaatca ttttcaacaa aaaaaccgaa tacaaatgta ttcggcctaa aaaagtattt
    28501 acgctttttc tttatgatct tgcttgcgtt ttctaaacaa tagtaatgat cctaataatg
    28561 ccatcattgc accaaataaa gttgcatttg tgttttcgct cttatctcct gtttctggta
    28621 aagcatcagt tttgtgttgt tttgatacct tattagaatg gtttacttca cctttaggat
    28681 ttgatggtgc tttctgttca ttatttggtg gtgtaactct tgaatcggag tcactatctg
    28741 agtctgagtc gctatctgaa tccgagtcgc tatccgagtc tgagtcgcta tctgagtctg
    28801 aatcgctgtc tgagtctgag tc
//
LOCUS       DALAGF010000022        28570 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.022, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000022 DALAGF010000000
VERSION     DALAGF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28570
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.022"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            104..1441
                     /locus_tag="O2692_000932"
     CDS             104..1441
                     /locus_tag="O2692_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCZ4298570.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKFLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            1443..2060
                     /locus_tag="O2692_000933"
     CDS             1443..2060
                     /locus_tag="O2692_000933"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCZ4298571.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2088..2417
                     /locus_tag="O2692_000934"
     CDS             2088..2417
                     /locus_tag="O2692_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCZ4298572.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            2631..3557
                     /locus_tag="O2692_000935"
     CDS             2631..3557
                     /locus_tag="O2692_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCZ4298573.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQNMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            3558..4361
                     /locus_tag="O2692_000936"
     CDS             3558..4361
                     /locus_tag="O2692_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCZ4298574.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIEDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            4378..4725
                     /gene="yabA"
                     /locus_tag="O2692_000937"
     CDS             4378..4725
                     /gene="yabA"
                     /locus_tag="O2692_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCZ4298575.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            4999..5724
                     /locus_tag="O2692_000938"
     CDS             4999..5724
                     /locus_tag="O2692_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCZ4298576.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            5717..5965
                     /locus_tag="O2692_000939"
     CDS             5717..5965
                     /locus_tag="O2692_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCZ4298577.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            5967..6806
                     /gene="rsmI"
                     /locus_tag="O2692_000940"
     CDS             5967..6806
                     /gene="rsmI"
                     /locus_tag="O2692_000940"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCZ4298578.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            7091..9064
                     /gene="metG"
                     /locus_tag="O2692_000941"
     CDS             7091..9064
                     /gene="metG"
                     /locus_tag="O2692_000941"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCZ4298579.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKSGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLNES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATKEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9095..9868
                     /locus_tag="O2692_000942"
     CDS             9095..9868
                     /locus_tag="O2692_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCZ4298580.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10035..10571
                     /gene="rnmV"
                     /locus_tag="O2692_000943"
     CDS             10035..10571
                     /gene="rnmV"
                     /locus_tag="O2692_000943"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCZ4298581.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHAELIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            10582..11475
                     /gene="rsmA"
                     /locus_tag="O2692_000944"
     CDS             10582..11475
                     /gene="rsmA"
                     /locus_tag="O2692_000944"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCZ4298582.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            11575..11838
                     /locus_tag="O2692_000945"
     CDS             11575..11838
                     /locus_tag="O2692_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCZ4298583.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            12148..12996
                     /locus_tag="O2692_000946"
     CDS             12148..12996
                     /locus_tag="O2692_000946"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCZ4298584.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            13010..13834
                     /gene="purR"
                     /locus_tag="O2692_000947"
     CDS             13010..13834
                     /gene="purR"
                     /locus_tag="O2692_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCZ4298585.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            13851..14231
                     /locus_tag="O2692_000948"
     CDS             13851..14231
                     /locus_tag="O2692_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCZ4298586.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNIDGHIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            14303..14605
                     /gene="spoVG"
                     /locus_tag="O2692_000949"
     CDS             14303..14605
                     /gene="spoVG"
                     /locus_tag="O2692_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HCZ4298587.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            14795..14926
                     /locus_tag="O2692_000950"
     CDS             14795..14926
                     /locus_tag="O2692_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298588.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            14949..16301
                     /gene="glmU"
                     /locus_tag="O2692_000951"
     CDS             14949..16301
                     /gene="glmU"
                     /locus_tag="O2692_000951"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCZ4298589.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            16448..17413
                     /locus_tag="O2692_000952"
     CDS             16448..17413
                     /locus_tag="O2692_000952"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCZ4298590.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            17563..18216
                     /locus_tag="O2692_000953"
     CDS             17563..18216
                     /locus_tag="O2692_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCZ4298591.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            18527..19099
                     /locus_tag="O2692_000954"
     CDS             18527..19099
                     /locus_tag="O2692_000954"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCZ4298592.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            19099..22605
                     /gene="mfd"
                     /locus_tag="O2692_000955"
     CDS             19099..22605
                     /gene="mfd"
                     /locus_tag="O2692_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCZ4298593.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNVEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            22595..24121
                     /locus_tag="O2692_000956"
     CDS             22595..24121
                     /locus_tag="O2692_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCZ4298594.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDGHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLYAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRITGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            24121..25314
                     /locus_tag="O2692_000957"
     CDS             24121..25314
                     /locus_tag="O2692_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCZ4298595.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHNQFEDVYIDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVIFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            25311..25574
                     /locus_tag="O2692_000958"
     CDS             25311..25574
                     /locus_tag="O2692_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCZ4298596.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            25592..25984
                     /locus_tag="O2692_000959"
     CDS             25592..25984
                     /locus_tag="O2692_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCZ4298597.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            26089..26490
                     /locus_tag="O2692_000960"
     CDS             26089..26490
                     /locus_tag="O2692_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCZ4298598.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            26670..27965
                     /gene="tilS"
                     /locus_tag="O2692_000961"
     CDS             26670..27965
                     /gene="tilS"
                     /locus_tag="O2692_000961"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCZ4298599.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLETYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE"
     gene            27970..28509
                     /gene="hpt"
                     /locus_tag="O2692_000962"
     CDS             27970..28509
                     /gene="hpt"
                     /locus_tag="O2692_000962"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCZ4298600.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
BASE COUNT        10343 a         3835 c         5690 g         8702 t
ORIGIN      
        1 ttgcataagt aacactaact tttgtcaaag aagtgttact atataattaa tacttttgaa
       61 agtaactaat tccaaaacag tgtaaataaa ggaagcgtat atcatgaagc aacctatttt
      121 aaataaatta gaaagtttaa atcaagaaga agcgatttct ttgcatgttc cgggtcataa
      181 aaatatgact atcggtcatt tatctcaatt atcaatgaca atggataaaa ctgaaatacc
      241 tggattagat gatttacatc atcctgaaga agtcattttg gaaagtatga agcaggtgga
      301 gaaacattca gattatgatg cttatttctt agtgaatggc accacttcag gaatattatc
      361 tgtcatccag tctttttcac agaaaaaagg cgatatctta atggcaagaa atgtacataa
      421 atctgtgtta catgcgctcg atattagcca acaagaaggg cattttattg aaacgcatca
      481 aagtccgtta acgaatcatt ataataaagt taatttaagc cgtttgaata atgacggtca
      541 caaacttgct gtgttgactt atcctaacta ttacggtgaa acatttaatg tagaagaggt
      601 tatcaaattt ttgcaccaat taaatattcc tgtactcatt gacgaagcac acggcgcgca
      661 ctttggattg caaggatttc cagattctac attaaattat caagctgact atgttgttca
      721 atcttttcat aaaacgttac cagctttaac gatgggctcg gtactttata ttcataaaaa
      781 tgcaccttat agagaaacta ttatagaata tctaagctac ttccaaacat ctagtccttc
      841 gtatttgatt atggctagtt tagagtcagc tgccgagttc tataaaacat atgatagtac
      901 cgtgtttttt gataagagag cgcaattaat cgaatgtttg gagaagaagg gttttgaaat
      961 gcttcaagtt gatgatccgt tgaagttgct gataaaatat gaaggtttta caggtcatga
     1021 tattcaaaat tggtttatga atgcacatat ctatttagaa ttagcggacg actatcaagc
     1081 attagcgata ttgccgttat ggcatcatga tgatacgtat ttatttgatt cgcttttacg
     1141 taaaattgaa gatatgattt taccgaaaaa atcagtttct aaagttaaac aaacacaact
     1201 tttaacaact gaaggtaact ataaaccaaa acgctttgaa tatgttactt ggtgtgattt
     1261 gaaaaaggca aaaggtaaag ttctggcgcg acatattgtc ccgtatccgc cagggattcc
     1321 tattattttc aaaggagaaa caataactga aaatatgata gaattggtaa atgaatatct
     1381 ggaaactgga atgatagttg aaggaattaa aaataataaa attttagttg aggatgaata
     1441 aaatgtcagc ttttataact tttgagggcc cagaaggctc tggaaaaaca actgtaatta
     1501 atgaagttta ccatagatta gtaaaagatt atgatgtcat tatgactaga gaaccaggtg
     1561 gtgttcctac tggtgaagaa atacgtaaaa ttgtattaga aggcaatgat atggacatta
     1621 gaactgaagc aatgttattt gctgcatcta gaagagaaca tcttgtatta aaggtcatac
     1681 cagctttaaa agaaggtaag gttgtgttgt gtgatcgcta tatcgatagt tcattagctt
     1741 atcaaggtta tgctagaggg attggcgttg aagaagtaag agcattaaac gaatttgcaa
     1801 taaatggatt atatccagac ttgacgattt atttaaatgt tagtgctgaa gtaggtcgcg
     1861 aacgtattat taaaaattca agagatcaaa atagattaga tcaagaagat ttaaagtttc
     1921 acgaaaaagt aattgaaggt taccaagaaa tcattcataa tgaatcacaa cggttcaaaa
     1981 gcgttaatgc agatcaacct cttgaaaatg ttgttgaaga cacgtatcaa actatcatca
     2041 aatatttaga aaagatatga tataattgtt agaagaggtg ttataaaatg aaaatgatta
     2101 tagcgatcgt acaagatcaa gatagtcagg aacttgcaga tcaacttgtt aaaaataact
     2161 ttagagcaac aaaattggca acaacaggtg ggtttttaag agcgggtaat acaacattct
     2221 tatgtggtgt caatgatgac cgtgtagatg aaatattgtc tgtgattaat caaacgtgtg
     2281 gtaatagaga acagttggtt tcacctatta cacctatggg aggcagtgcg gattcgtaca
     2341 ttccatatcc agttgaagtt gaagttggcg gtgctactgt atttgttatg ccagttgatg
     2401 cattccatca attttaattc tataatacaa tcatcaatta gagatactta aaatagtgta
     2461 ttaataagtt attaacaatt ttgggttgct tgactgcgac tagttcagat gccaatagat
     2521 ttgatttttg tggttctaaa aataatcaca aatcatggtc gctattgttg cagtaattag
     2581 ttgctctttg gcaacctttt tatataaaag caaaagggag tttgtaatga atggatgaac
     2641 agcaacaatt gacgaatgca tatcattcaa ataaattatc gcatgcctat ttatttgaag
     2701 gtgatgatgc acaaacgatg aaacaagttg cgattaattt tgcaaagctt attttatgtc
     2761 aaacagatag tcaatgtgaa acaaaggtta gtacatataa tcatccagac tttatgtata
     2821 tatcaacaac tgagaatgca attaagaaag aacaagttga acaacttgtg cgtcatatga
     2881 atcaacttcc tatagaaagc acaaataaag tgtacatcat tgaagacttt gaaaagttaa
     2941 ctgttcaagg ggaaaacagt atcttgaaat ttcttgaaga accaccggac aatacgattg
     3001 ctattttatt gtctacaaaa cctgagcaaa ttttagacac aatccattca agatgtcagc
     3061 atgtatattt caagcctatt gataaagaaa agtttataaa tagattagtt gaacaaaaca
     3121 tgtctaagcc agtagctgaa atgattagta cttatactac gcaaatagat aatgcaatgg
     3181 ctttaaatga agaatttgat ttattagcat taaggaaatc agttatacgt tggtgtgaat
     3241 tgttgcttac taataagcca atggcactta taggtattat tgatttattg aaacaggcta
     3301 aaaataaaaa actgcaatct ttaactattg cagctgtgaa tggtttcttc gaagatatca
     3361 tacatacaaa ggtaaatgta gaggataaac aaatatatag tgatttaaaa aatgatattg
     3421 atcaatatgc gcaaaagttg tcgtttaatc aattaatttt gatgtttgat caactgacgg
     3481 aagcacataa gaaattgaat caaaatgtaa atccaacgct tgtatttgaa caaatcgtaa
     3541 ttaagggtgt gagttagatg ccaaatgtaa taggtgttca gtttcaaaaa gcgggaaaat
     3601 tagaatatta tacacctaat gatatacaag tagatataga agactgggta gttgtcgaat
     3661 ctaaaagagg catagagata ggtattgtta aaaatccatt aatggatatt gctgaagagg
     3721 atgttgtgtt acctcttaaa aatattattc gcattgctga tgacaaagat attgataaat
     3781 ttaattgtaa tgaacgagat gctgaaaatg cattaatact atgtaaagac attgtaagag
     3841 aacaaggttt ggacatgcgt ttagtcaatt gcgaatatac attagataaa tcgaaagtta
     3901 tttttaattt tacggcggat gatcgtattg attttagaaa attagtaaaa atattagcgc
     3961 aacatttaaa aacacgtatc gagttgagac aaattggtgt aagggatgaa gccaaattgc
     4021 ttggcggtat cggaccttgt ggtaggtcgt tatgttgttc tacattttta ggggattttg
     4081 aaccagtatc gattaagatg gctaaggatc aaaatttatc attaaatcca actaaaattt
     4141 ctggtgcatg tggtcgtttg atgtgttgtt taaaatatga aaatgactat tatgaggaag
     4201 tacgtgcaca attacctgat attggtgaag caattgaaac gcctgatggt aacgggaaag
     4261 tagttgcttt aaatatatta gacatttcta tgcaggtgaa gcttgaggga catgaacagc
     4321 cacttgaata taaattagaa gaaatagaaa ctatgcatta aggaggcatt attacatttg
     4381 gatcgcaatg aaatatttga aaaaataatg cgtttagaaa tgaatgtcaa tcaactttca
     4441 aaggaaactt cagaattaaa ggcacttgca gttgaattag tagaagaaaa tgtagcgctt
     4501 caacttgaaa atgataattt gaaaaaggtg ttgggcaatg atgaaccaac tactattgat
     4561 actgcgaatt caaaaccagc aaaagctgtg aaaaagccat taccaagtaa agataatttg
     4621 gctatattgt atggagaagg atttcatatt tgtaaaggcg aattatttgg aaaacatcga
     4681 catggtgaag attgtctgtt ctgtttagaa gttttaagtg attaatcaag cacactcaaa
     4741 tagtgttata attataaatg aatatggttt ggataagtct gagacaatgc atgtttcagg
     4801 ctttaattgt gtataaagtt ttggtgattg cataagagat ggcggtacta aatgttatta
     4861 ttaagtgtgc acgcagtatc attagttata aaatgtagct gttaaaagtc aaaaatacat
     4921 cgaatgtagt taggcatata atataaaaag agttttcaat tactcaatag aaaaaggttg
     4981 tcttcatagg agttaaaaat gttaaaagag aatgaacgat ttgatcaact aatcaaagaa
     5041 gattttagta ttattcaaaa tgatgatgtt ttttcatttt caacggatgc tttgttgtta
     5101 gggcatttta caaaacctag aacaaaagat attgtgttgg acttatgttc aggcaatggg
     5161 gtgataccct tgttattgtt tgcgaaacat ccacgacata tagaaggtgt tgagattcaa
     5221 aaaacacttg tcgatatggc gcgacgcaca tttcaattca atgatgttga tgaatattta
     5281 acaatgcatc acatggattt gaaaaacgtt actaaagtat ttaaaccttc acaatatact
     5341 ttagtaacgt gtaatccgcc ttattttaaa gagaatcagc aacaccaaca tcaaaaagaa
     5401 gcacataaga tagcgagaca tgagattatg tgtacacttg aagattgcat gattgcagcc
     5461 cgtcatttat taaaagaagg tggcaggcta aacatggtac atcgtgcaga gagactaatg
     5521 gatgtcttgt ttgaaatgag aaaagtgaat attgaaccta agaaagtcgt ttttatatat
     5581 agtaaagtag ggaaatcagc acaaacgata gtagtagaag gtcgaaaagg tggaaatcaa
     5641 ggtttagaaa tcatgccccc attttatatt tataatgaag atggtaatta tagcgaagaa
     5701 atgaaggaag tatattatgg atagtcattt tgtatatatt gtaaaatgta gtgatggaag
     5761 tttatataca ggatacgcta aagacgttaa tgcacgtgtt gaaaaacata accgaggtca
     5821 aggagccaaa tatacgaaag taagacgtcc ggtgcattta gtttatcaag aaatgtatga
     5881 gacaaagtct gaagcattga agcgtgaata tgaaattaaa acttatacca gacaaaagaa
     5941 attgcgatta attaaggagc gatagtatgg ctgtattata tttagtgggc acaccaattg
     6001 gtaatttagc agatattact tatagagcag ttgatgtatt gaaacgtgtt gatatgattg
     6061 cttgtgaaga cactagagta actagtaaac tgtgtaatca ttatgatatt ccaactccat
     6121 taaagtcata tcacgaacat aacaaggata agcagactgc ttttatcatt gaacagttag
     6181 aattaggtct tgacgttgcg ctcgtatctg atgctggatt gcccttaatt agtgatcctg
     6241 gatacgaatt agtagtggca gccagagaag ctaatattaa agtagagacc gtgcctggac
     6301 ctaatgctgg gctgacggct ttgatggcta gtggattacc ttcatatgta tatacatttt
     6361 taggattttt gccacgaaaa gagaaagaaa aaagtgctgt attagagcaa cgtatgcatg
     6421 aaaatagcac attaattata tacgaatcac cgcatcgtgt gacagataca ttaaaaacaa
     6481 ttgcaaagat agatgcaaca cgacaagtat cactagggcg tgaattaact aagaagttcg
     6541 aacaaattgt aactgatgat gtaacacaat tacaagcatt gattcagcaa ggcgatgtac
     6601 cattgaaagg cgaattcgtt atcttaattg aaggtgctaa agcgaacaat gagatatcgt
     6661 ggtttgatga tttatctatc aatgagcatg ttgatcatta tattcaaact tcacagatga
     6721 aaccaaaaca agctattaaa aaagttgctg aagaacgaca acttaaaacg aatgaagtat
     6781 ataatattta tcatcaaata agttaatcac tttatcgatt atatgaaatt ttaaacgatt
     6841 ttataaacgc aagctgtaat tttaaatggt aagttatcat tttgcattga tactgataaa
     6901 atgatgttga ctatgataaa aaaatgatga catcgacgtt ttttaatgta aaataaatac
     6961 attgaaagta ataaatacct taacattgaa taagatgaaa atgagatgac gagataaatg
     7021 ttcgcgtccg ttgaaatgca tagaaatctt agatattatt tgaagtgaga cattacgagg
     7081 aggaacagtt atggctaaag aaacatttta tataacaacc ccaatatact atcctagtgg
     7141 gaatttacat ataggacatg catattctac agtggctgga gatgttattg caagatataa
     7201 gagaatgcaa ggatatgatg ttcgctattt gactggaacg gatgaacacg gtcaaaaaat
     7261 tcaagaaaaa gctcaaaaat ctggtaagac agaaattgaa tatttggatg agatgattgc
     7321 tggaattaaa caattgtggg ctaagcttga aatttcaaat gatgatttta tcagaacaac
     7381 tgaagaacgt cataaacatg tcgttgagca agtgtttgaa cgtttattaa agcaaggtga
     7441 tatctattta ggtgaatatg aaggttggta ttctgttccg gatgaaacat actatacaga
     7501 gtcacaatta gtagacccac aatacgaaaa cggtaaaatt attggtggca aaagtccaga
     7561 ttctggacac gaagttgaac tagttaaaga agaaagttat ttctttaata ttagtaaata
     7621 tacagaccgt ttattagagt tctatgacca aaatccagat tttatacaac caccatcaag
     7681 aaaaaatgaa atgattaaca acttcattaa accaggactt gctgatttag ctgtttctcg
     7741 tacatcattt aactggggtg tccatgttcc gtctaatcca aaacatgttg tttatgtttg
     7801 gattgatgcg ttagttaact atatttcagc attaggctat ttatcagatg atgagtcact
     7861 atttaacaaa tactggccag cagatattca tttaatggct aaggaaattg tgcgattcca
     7921 ctcaattatt tggcctattt tattgatggc attagactta ccgttaccta aaaaagtctt
     7981 tgcacatggt tggattttga tgaaagatgg aaaaatgagt aaatctaaag gtaatgtcgt
     8041 agaccctaat attttaattg atcgctatgg tttagatgct acacgttatt atctaatgcg
     8101 tgaattacca tttggttcag atggcgtatt tacacctgaa gcatttgttg agcgtacaaa
     8161 tttcgatcta gcaaatgact taggtaactt agtaaaccgt acgatttcta tggttaataa
     8221 gtactttgat ggcgaattac cagcgtatca aggtccactt catgaattag atgaagaaat
     8281 ggaagctatg gctttagaaa cagtgaaaag ctacactgaa agcatggaaa gtttgcaatt
     8341 ttctgtggca ttatctacgg tatggaagtt tattagtaga acgaataagt atattgacga
     8401 aacaacgcct tgggtattag ctaaggacga tagccaaaaa gatatgttag gcaatgtaat
     8461 ggctcactta gttgaaaata ttcgttatgc agctgtatta ttacgtccat tcttaacaca
     8521 tgcgccgaaa gagatttttg aacaattgaa cattaacaat cctcaattta tggaatttag
     8581 tagtttagag caatatggtg tgcttaatga gtcaattatg gttactgggc aacctaaacc
     8641 tattttccca agattggata gcgaagcgga aattgcatat atcaaagaat caatgcaacc
     8701 gcctgctact aaagaggaaa aagaagagat tcctagcaaa cctcaaattg atattaaaga
     8761 ctttgataaa gttgaaatta aggcagcaac gattattgat gctgaacatg ttaagaagtc
     8821 agataagctt ttaaaaattc aagtagactt agattctgaa caaagacaaa ttgtatcagg
     8881 aattgccaaa ttctatacac cagatgatat tattggtaaa aaagtagcag ttgttactaa
     8941 cctgaaacca gctaaattaa tgggacaaaa atctgaaggt atgatattat ctgctgaaaa
     9001 agatggtgta ttaaccttag taagtttacc aagtgcaatt ccaaatggtg cagtgattaa
     9061 ataactgtat ttttaaaaat taggagagat aattatgtta atcgatacac atgtccattt
     9121 aaatgatgag caatacgatg atgatttgag tgaagtgatt acacgtgcta gagaagcagg
     9181 tgttgatcgt atgtttgtag ttggttttaa caaatcgaca attgaacgcg cgatgaaatt
     9241 aatcgatgag tatgattttt tatatggcat tatcggttgg catccagttg acgcaattga
     9301 ttttacagaa gaacacttgg aatggattga atctttagct cagcatccaa aagtgattgg
     9361 tattggtgaa atgggattag attatcactg ggataaatct cctgcagatg ttcaaaagga
     9421 agtttttaga aagcaaattg ctttagctaa gcgtttgaag ttaccaatta tcattcataa
     9481 ccgtgaagca actcaagact gtatcgatat cttattggag gagcatgctg aagaggtagg
     9541 cgggattatg catagcttta gtggttctcc agaaattgca gatattgtaa ctaataagct
     9601 gaatttttat atttcattag gtggacctgt gacatttaaa aatgctaaac agcctaaaga
     9661 agttgctaag catgtgtcaa tggagcgttt gctagttgaa accgatgcac cgtatctttc
     9721 gccacatccg tatagaggga agcgaaatga accggcgaga gtaactttag tagctgaaca
     9781 aattgctgaa ttaaaaggct tatcttatga agaagtgtgc gaacaaacaa ctaaaaatgc
     9841 agagaaattg tttaatttaa attcataaag ttaaaagtga gaaagatcac cgccataaat
     9901 gtaaacgatg ctatattcgt ttaatatgct atggttcttt ctcacttttt taaattaaaa
     9961 tatcgtgcat gtggaatacg tgcgatagag atggttagag ctttgaaatt aagaattgta
    10021 ggaaggcgtt ttaaatgaaa atcaatgagt ttatagttgt agaaggacga gatgatactg
    10081 agcgtgttaa acgagctgtt gaatgtgata cgattgaaac gaatggtagt gccatcaacg
    10141 aacaaacttt agaagtaatt agaaatgctc aacaaagtcg aggcgttatt gtattaacag
    10201 atccagattt cccaggagat aaaattagaa gtacaattac tgaacatgtc aaaggtgtta
    10261 aacatgcgta tattgataga gaaaaagcta aaaataaaaa agggaaaatt ggtgttgaac
    10321 atgccgaatt aattgatatt aaagaagcgt taatgcatgt tagttcaccc tttgatgaag
    10381 cttatgaatc aattgataaa tctgtgctaa tagagttggg gttaattgtt gggaaagatg
    10441 caaggcgccg tagagaaatt ttaagtagaa aattgcgaat cggccattcc aatggtaagc
    10501 agttattgaa aaagttaaat gcatttggtt ataccgaagc ggatgtaagg caagctttag
    10561 aagatgaatg aggaagtgaa aatgttggat aataaagata ttgcaacacc atcaagaacg
    10621 cgagcgttgt tagataaata tggctttaat tttaaaaaaa gtttaggaca gaactttttg
    10681 atagatgtga atatcattaa taatatcatt gatgcaagtg atattgatgc acaaactggg
    10741 gtgattgaaa ttggtccagg catggggtca ttgacagaac aattggccag acatgctaaa
    10801 agagtattgg catttgaaat tgatcaacgt ttaatacctg tattaaatga tacactatca
    10861 ccttatgata atgtgacggt gattaatgaa gatattttaa aagcgaatat taaagaagct
    10921 gttgaaaatc atttacaaga ttgtgaaaaa ataatggttg ttgcaaacct gccgtactat
    10981 attacgacgc caattttatt aaatttgatg caacaagata taccaattga tggctacgtg
    11041 gtgatgatgc aaaaagaagt gggcgaacgc ttaaatgctg aagtaggttc aaaagcatat
    11101 ggttcgttat caattgtcgt acaatactat acagagacta gtaaagtatt aacggtacct
    11161 aaatctgtat ttatgccacc acctaatgtt gattcaatag ttgtaaaact gatgcagaga
    11221 actgaaccgt tagtaacagt agataacgag gaagcattct ttaagttagc aaaagcagca
    11281 tttgcacaaa gaagaaagac aattaacaat aactatcaaa attattttaa agatggtaaa
    11341 caacacaaag aagtgatttt acaatggttg gaacaagcag gtattgatcc aagacgtcgc
    11401 ggtgaaacgc tatctattca agattttgct aaattgtatg aagaaaagaa aaaattccct
    11461 caattagaaa attaaatgat tgacaaagca aagcactatt gttaaaattt aaattttgtt
    11521 tgacgaaaac gttgcaaata tggtattatg taacttgtag cgaggtggag caatatgcca
    11581 aaatcaattt tggacatcaa aaattctatt gattgtcatg taggaaatcg tattgtactg
    11641 aaagccaatg gaggccgtaa gaaaacaata aaacgttctg gaattttaaa agaaacatat
    11701 ccgtcagttt tcattgttga gttagatcaa gacaaacaca actttgagag agtatcttat
    11761 acatacactg atgtgttaac tgaaaatgtt caagtttcat ttgaagagga taatcatcac
    11821 gaatcaattg cacactaaat aagacatata gagatgttag acgtttctta gtataagaag
    11881 taaatattat gataattatt tgagtgttgg gcattatgtt caatactctt tttatttaca
    11941 aaatgtttaa cactgatgtt tcgcttatag atttttcagt aaatggataa ttgtatttat
    12001 aaacacaaat acaagtaaat actaagtaat tagatggaga aaattacttt tttattaaaa
    12061 aaacactaaa aaacaaatta aaatgtcaaa tattaattct ctttatgtta aaatcatcat
    12121 attaagataa cgaaaagagg gcggaaaatg atatatgaaa cggcaccagc caaaattaat
    12181 tttacgctcg atacactttt taaaagaaat gatggctatc atgagattga aatgataatg
    12241 acaacagttg atttaaatga tcgtttaact tttcataaaa gaaaagatcg aaagatagtt
    12301 gttgagattg aacataatta tgtgccttct aatcataaaa atctcgcata tcgtgcagcg
    12361 caactattta ttgagcaata tcaactaaag caaggtgtaa caatttctat cgataaagaa
    12421 atacctgttt ctgctggctt agctggaggt tcggctgatg cagcagcaac gttaagagga
    12481 ttgaatcgac tttttgatat aggggcgagt ttggaagaat tggctctact aggcagtaaa
    12541 atcgggacag atattccgtt ttgtatttat aataaaactg cactatgtac tggaagagga
    12601 gagaaaatcg agtttttaaa taaaccacct tcagcttggg tgattcttgc taaaccaaac
    12661 ttaggcatat catcaccaga tatatttaag ttgattaatt tagataagcg ttacgacgta
    12721 catacgaaaa tgtgttatga ggccttagaa aatcgagatt atcaacaatt atgtcaaagt
    12781 ttgtctaatc gattagagcc aatttctgtt tcaaaacacc cacaaatcga taaattaaaa
    12841 aataatatgt tgaaaagtgg tgcagatggt gcgttaatga gtggaagcgg acctactgtg
    12901 tatgggctag cacgaaaaga aagccaagca aaaaatattt ataatgcagt taacggttgt
    12961 tgtaatgaag tgtacttagt tagactatta ggatagaagg gttgaaaaga tgagatataa
    13021 acgaagcgag agaattgttt ttatgacgca atatttgatg aaccatccga ataaattgat
    13081 tccattaact ttttttgtga aaaaatttaa acaggcgaag tcttcaataa gtgaagatgt
    13141 ccaaattata aaaaatacat tccaaaaaga aaagttaggt acagtaatta ctactgctgg
    13201 cgcaagtggt ggtgttacgt ataaaccaat gatgagtaaa gaagaggcga ctgaagttgt
    13261 taatgaggtc attactctat tagaagagaa agaacgtttg ttacctggcg gatatttatt
    13321 tttatcagat ttggtaggta atccatcgct actaaacaaa gttggtaagt taattgccag
    13381 tatttacatg gaagaaaaat tagatgctgt tgttaccatt gcgacaaaag gtatttcatt
    13441 ggcaaatgcg gttgctaata ttttaaattt accagtagta gtgattagaa aagacaacaa
    13501 ggtgactgaa ggttctacag tttcaattaa ttacgtttca ggatcttcaa gaaaaataga
    13561 aacaatggta ctttcgaaga gaactttagc agaaaattca aatgttttag ttgtcgatga
    13621 ttttatgagg gctggtggct ctattaatgg tgttatgaat ttaatgaatg agtttaaagc
    13681 ccatgtaaaa ggggtatcag tacttgtaga atcaaaagaa gttaaacaaa gattgattga
    13741 agattatact tccttagtga aattatctga tgtagatgaa tataatcaag agtttaacgt
    13801 agaacctggc aacagtttat ctaagttttc ataaaaggag ttttagtatt atgaaaatca
    13861 ttaacacaac aagattaccg gaagcacttg gaccatattc gcatgcaaca gttgtgaatg
    13921 gtatggttta tacttctggt cagattccat tgaatattga tggacatatc gtaagcgctg
    13981 atgttcaagc acagacaaaa caagttttag aaaatttaaa ggttgttttg gaagaagcag
    14041 gatctgattt gaattctgtt gcgaaagcga ccattttcat taaagatatg aatgatttcc
    14101 aaaaaataaa tgaagtgtat ggtcaatatt ttaatgaaca caagccagcg cgtagttgtg
    14161 tagaggttgc gcgtttgcca aaagatgtga aagtagaaat tgaattagta agtaaaatta
    14221 aggaattata attttcgatt aatatgttta atcaagcttc taaataaaac agagagatat
    14281 atactatagg ggggctcact acatgaaagt gacagatgta agacttagaa aaatacaaac
    14341 agatggacga atgaaagcac tcgtttccat tacattagat gaagctttcg taattcatga
    14401 tttacgtgta attgaaggaa actctggctt gttcgttgca atgccaagta aacgtacacc
    14461 agatggtgaa ttccgcgaca tcgcgcatcc tattaattca gatatgagac aagaaattca
    14521 agatgcagtg atgaaagtat atgatgaaac agatgaagta gtaccagata aaaacgctac
    14581 atcagaagat tcagaagaag cttaatcaat tttatattta gcgatgtaat acatttgcaa
    14641 taagttgatt tgatactgtc gataaagcat aaagctttgt cggcagtttt tttagtttgt
    14701 attaatgttt ttttattttt aatgaaaggc taataaatat atacgttaac agattatgat
    14761 gatatgaaaa ttattgattc attgctgata aaaattgcgt ttgaatgatg ctcgtatttt
    14821 tgaagtaaga aaaaagttgt ttttaaaatt acaacgaatt aaaaacaatg ccttttatat
    14881 gttgaaagag tattgcagat taaattataa taatgacgaa gtgtaaaatt taatgggggt
    14941 taatgttcat gcgaagacac gcgataattt tggcagcagg taaaggcaca agaatgaaat
    15001 ctaaaaagta taaagtgcta cacgaggttg ctgggaaacc tatggtcgaa catgtattgg
    15061 aaagtgtgaa aggctctggt gtcgatcaag ttgtaaccat cgtaggacat ggtgctgaaa
    15121 gtgtaaaagg acatttaggc gagcgttctt tatacagttt tcaagaggaa caactcggta
    15181 ctgcgcatgc agtgcaaatg gcgaaatcac acttagaaga caaggaaggt acgacaatcg
    15241 ttgtatgtgg tgacacaccg ctcatcacaa aggaaacatt agaaacattg attgcgcatc
    15301 acgaggatgc taatgctcaa gcaactgtat tatctgcatc gattcaacaa ccatatggat
    15361 acggaagaat cgttcgaaat gcgtcaggtc gtttagaacg catagttgaa gagaaagatg
    15421 caacgcaagc tgaaaaggat attaatgaaa ttagttcagg tatttttgcg tttaataata
    15481 aaacgttgtt tgaaaaatta acacaagtga aaaatgataa tgcgcaaggt gaatattacc
    15541 tccctgatgt attgtcgtta attttaaatg atggcggcat cgtagaagtc tatcgtacca
    15601 atgatgttga agaaatcatg ggtgtaaatg atcgtgtaat gcttagtcag gctgagaagg
    15661 cgatgcaacg tcgtacgaat cattatcaca tgctaaatgg tgtgacaatc atcgatcctg
    15721 acagcactta tattggtcca gacgttacaa ttggtagtga tacagtcatt gaaccaggcg
    15781 tacgaattaa tggtcgtaca gaaattggcg aagatgttgt tattggtcag tactctgaaa
    15841 ttaacaatag tacgattgaa aatggtgcat gtattcaaca gtctgttgtt aatgatgcta
    15901 gcgtaggagc gaatactaag gtcggaccgt ttgcgcaatt gagaccaggc gcgcaattag
    15961 gtgcagatgt taaggttgga aattttgtag aaattaaaaa agcagatctt aaagatggtg
    16021 ccaaggtttc acatttaagt tatattggcg atgctgtaat tggcgaacgt actaatattg
    16081 gttgcggaac gattacagtt aactatgatg gtgaaaataa atttaaaact atcgtcggca
    16141 aagattcatt tgtaggttgc aatgttaatt tagtagcacc tgtaacaatt ggtgatgatg
    16201 tattggtggc agctggttcc acaatcacag atgacgtacc aaatgacagt ttagctgtgg
    16261 caagagcaag acaaacaaca aaagaaggat ataggaaata atcatttacg tatttaaaat
    16321 ggctaggata aaaggataat cctatgtaat attaatgtaa tctttatgat ttaatgattc
    16381 gcatagtaat ggagttacat tttatatata atagtaattg cgtaagtaaa taattggagg
    16441 actataaatg ttaaataatg aatataagaa ttcgtcatta aagatttttt cattgaaagg
    16501 aaacgaagca ttagcgcaag aagttgctga ccaagtagga attgaactag gtaaatgttc
    16561 agttaaacgt tttagtgatg gagaaattca aattaatatc gaagagagta ttcgtggttg
    16621 tgacgtattt attattcaac caacatcata tcctgtgaat ctacatttaa tggaattatt
    16681 aattatgatt gatgcttgta aacgtgcttc tgcagcaaca atcaatattg tagtgccata
    16741 ttatggatat gcaagacaag atagaaaagc ccgtagccgt gagccaatca ctgctaaatt
    16801 agttgcaaac ttaatcgaaa cagctggcgc aactcgtatg attgcgttag acttacatgc
    16861 accacaaatt caaggattct ttgatattcc aattgaccac ttaatgggtg tgccaattct
    16921 tgctaaacat ttcaaagatg atccgaatat taacccagaa gaatgtgtcg ttgtttcacc
    16981 agaccatggc ggcgttacac gtgcacgtaa attagctgac attttaaaaa ctccaattgc
    17041 aattatagat aaacgtcgtc ctagaccaaa tgttgctgaa gtgatgaaca ttgttggtga
    17101 gattgaagga cgtacggcaa ttattattga cgatattatt gatacagcag gtacaatcac
    17161 tttagctgca caagcattaa aagataaagg tgctaaagaa gtatatgctt gttgtacaca
    17221 ccctgtttta tcaggaccgg ctaaagaacg tatcgaaaat tctgctataa aagaattaat
    17281 cgtaacaaac tcaattcatt tagatgaaga tcgcaaacca tctaacacta aagaattatc
    17341 tgttgctggt ttaatcgcac aagctatcat tcgtgtatac gaaagagaat cagttagcgt
    17401 attatttgac taatatttaa aaggcgtttg acgaacatat tccaaacgtg tataatagtt
    17461 tcgttcgtga ttatacgaat aaataaacac ttgcaagcaa cgatgatgtt gatgggtaag
    17521 tgaggtgctc gttttgagca aaaatgaaag gtggaaatga gaatggcttc attaaagtca
    17581 atcatccgtc aaggtaaaca aacacgttca gatcttaaac aattaagaaa atctggtaaa
    17641 gtaccagcag tagtatacgg ttacggtact aaaaacgtgt cagttaaagt tgatgaagta
    17701 gaattcatca aagttatccg tgaagtaggt cgtaacggtg ttatcgaatt aggcgttggt
    17761 tctaaaacta tcaaagttat ggttgcagac taccaattcg atccacttaa aaaccaaatt
    17821 actcacattg acttcttagc aatcaatatg agtgaagaac gtactgttga agtaccagtt
    17881 caattagttg gtgaagcagt aggcgctaaa gaaggcggcg tagttgaaca accattattc
    17941 aacttagaag taactgctac tccagacaat attccagaag caatcgaagt agacattact
    18001 gaattaaaca ttaacgacag cttaactgtt gctgatgtta aagtaactgg cgacttcaaa
    18061 atcgaaaacg attcagctga atcagtagta acagtagttg ctccaactga agaaccaact
    18121 gaagaagaaa tcgaagctat ggaaggcgaa caacaaactg aagaaccaga agttgttggc
    18181 gaaagcaaag aagacgaaga aaaaactgaa gagtaatttt aatctgttac attaaagttt
    18241 ttatactttg tttaacaagc actgtgctta ttttaatata agcatggtgc tttttgtgtt
    18301 attataaagc ttaattaaac tttattactt tgtactaaag tttaattaat tttagtgagt
    18361 aaaagacatt aaactcaaca atgatacatc ataaaaattt taatgtactc gattttaaaa
    18421 tacatactta ctaagctaaa gaataatgat aattgatggc aatggcggaa aatggatgtt
    18481 gtcattataa taataaatga aacaattatg ttggaggtaa acacgcatga aatgtattgt
    18541 aggtctaggt aatataggta aacgttttga acttacaaga cataatatcg gctttgaagt
    18601 cgttgattat attttagaga aaaataattt ttcattagat aaacaaaagt ttaaaggtgc
    18661 atatacaatt gaacgaatga acggcgataa agtgttattt atcgaaccaa tgacaatgat
    18721 gaatttgtca ggtgaagcag ttgcaccgat tatggattat tacaatgtta atccagaaga
    18781 tttaattgtc ttatatgatg atttagattt agaacaagga caagttcgct taagacaaaa
    18841 aggaagtgcg ggcggtcaca atggtatgaa atcaattatt aaaatgcttg gtacagacca
    18901 atttaaacgt attcgtattg gtgtgggaag accaacgaat ggtatgacgg tacctgatta
    18961 tgttttacaa cgcttttcaa atgatgaaat ggtaacgatg gaaaaagtta tcgaacacgc
    19021 agcacgcgca attgaaaagt ttgttgaaac atcacgattt gaccatgtta tgaatgaatt
    19081 taatggtgaa gtgaaataat gacaatattg acaacgctta taaaagaaga taatcatttt
    19141 caagacctta atcaggtatt tggacaagca aacacactag taactggtct ttccccgtca
    19201 gctaaagtga cgatgattgc tgaaaaatat gcacaaagta atcaacagtt attattaatt
    19261 accaataatt tataccaagc agataaatta gaaacagatt tacttcaatt tatagatgct
    19321 gaagaattgt ataagtatcc tgtgcaagat attatgaccg aagagttttc aacacaaagc
    19381 cctcaactga tgagtgaacg tattagaact ttaactgcgt tagctcaagg taagaaaggg
    19441 ttatttatcg ttcctttaaa tggtttgaaa aagtggttaa ctcctgttga aatgtggcaa
    19501 aatcaccaaa tgacattgcg tgttggtgag gatatcgatg tggaccaatt tcttaacaaa
    19561 ttagttaata tggggtacaa acgggaatcc gtggtatcgc atattggtga attctcattg
    19621 cgaggaggta ttatcgatat ctttccgcta attggggaac caatcagaat tgagctattt
    19681 gataccgaaa ttgattctat tcgggatttt gatgttgaaa cgcagcgttc caaagataat
    19741 gttgaagaag tcgatatcac aactgcaagt gattatatca ttactgaaga agtgatcagc
    19801 catcttaaag aagagttaaa aactgcatat gaaaatacaa gacccaaaat agataaatca
    19861 gtgcgcaatg atttgaaaga aacgtatgaa agctttaaat tattcgaaag tacatacttt
    19921 gatcatcaaa tactacgtcg cttagtagcg tttatgtatg aaacaccttc gacaattatt
    19981 gagtatttcc aaaaagatgc aatcattgca gttgatgaat ttaatcgtat taaagaaact
    20041 gaagaaagtt taacagtaga gtctgattcg tttattagca atattattga aagtggtaat
    20101 ggatttatag gacaaagttt tataaaatat gatgattttg aaacattgat tgaaggctat
    20161 cctgtcactt atttttcatt attcgctaca acaatgccga taaaactaaa tcatattatt
    20221 aaattttcat gtaaacctgt ccaacaattt tatgggcaat atgacattat gcgttctgaa
    20281 tttcaacgat atgttaatca aaactatcat atcgtggttt tggtcgaaac cgaaactaaa
    20341 gttgaacgta tgcaagcgat gttaagtgaa atgcatattc catcaataac aaaattgcat
    20401 cgctcaatgt catcggggca agcagtgatt attgaaggca gtttatctga aggatttgaa
    20461 ctacctgata tgggattagt tgtcattact gagcgtgagc tttttaaatc aaaacagaaa
    20521 aagcaacgaa aacgtacgaa agctatctca aatgctgaaa aaattaagtc ttaccaagat
    20581 ttaaatgtgg gagattatat tgttcatgtg catcatggtg ttggtagata tttaggtgtt
    20641 gagacgctcg aagtggggca aacgcatcgt gattatatta aattgcaata taaaggtacg
    20701 gatcaactat ttgttccagt agatcaaatg gatcaagttc aaaaatatgt agcttcggaa
    20761 gataagacgc caaaattaaa taaactcggt ggcagtgaat ggaaaaaaac aaaagctaaa
    20821 gttcaacaaa gtgttgaaga tattgctgaa gagttgattg atttatataa agaaagagaa
    20881 atggcagaag gttatcaata tggggaagac acagctgagc aaacaacatt tgaattagat
    20941 tttccatatg aacttacgcc tgaccaagct aaatctatcg atgaaattaa agatgacatg
    21001 caaaaatcgc gtccaatgga tcgcttgcta tgtggtgatg ttggttatgg taaaactgaa
    21061 gttgcagtga gagcagcatt caaagctgta atggaaggaa agcaggttgc atttttagtt
    21121 cctacaacta ttttagctca gcaacattat gagacgttaa ttgagcgtat gcaagatttt
    21181 cctgttgaaa ttcaattaat gagtcgtttt agaacgccta aagagataaa acaaactaag
    21241 gaaggactta aaactggatt tgttgacata gttgttggta cacacaaatt acttagtaaa
    21301 gatatacagt ataaagattt agggctgttg attgtagatg aagaacaacg atttggtgta
    21361 cgccataaag agcgtattaa aacattaaaa cataatgtag atgtactaac attgactgca
    21421 accccaatac ctagaacatt gcatatgagt atgctaggtg tgcgggattt gtcagtgatt
    21481 gaaacgccgc cagaaaatcg tttcccagtt caaacatatg tattagaaca gaacatgagt
    21541 tttatcaaag aagctttaga aagagaacta tcccgtgatg gccaagtgtt ttatctttat
    21601 aataaagtgc aatccattta tgaaaaacga gaacaactcc agatgttaat gccagatgct
    21661 aacattgcag ttgctcatgg acaaatgaca gagcgcgatt tagaagaaac gatgttaagt
    21721 tttatcaata atgaatatga tattttagta acgacgacga ttattgaaac aggtgtcgat
    21781 gtcccaaatg caaatacttt gatcattgaa gatgcagatc gctttggatt gagtcagttg
    21841 tatcaattaa gaggtcgtgt tggtcgttca agtcgtattg gttatgcata cttcttacat
    21901 ccagcaaata aggtactaac tgagactgca gaagatcgat tacaagcgat taaagaattt
    21961 acggagttag gctcaggatt taagattgcg atgcgtgatt tgaacattcg tggtgctggt
    22021 aatttgttag gtaaacaaca gcacggcttt attgatacag ttggatttga tttgtacagt
    22081 caaatgttag aagaagctgt aaatgaaaaa cgtggtatta aggaaccaga atctgaggtg
    22141 ccagaagtcg aagttgattt aaacttggat gcatatttgc caacagaata tattgcaaat
    22201 gaacaagcta aaattgaaat ttataaaaag ctacgaaaaa ctgaaacatt tgatcaaatt
    22261 atcgacatta aagatgaatt aattgatcgt ttcaatgatt atcctgttga agtagcacgt
    22321 ttgcttgata tagtggaaat aaaagtacac gcattacatt caggtatcac gttgattaaa
    22381 gataaaggga aaataattga tattcattta tctgtaaaag ccactgaaaa tattgatggc
    22441 gaagtgctgt tcaaagcaac acaaccttta ggtagaacaa tgaaggttgg tgttcaaaat
    22501 aatgcaatga caattacttt aacgaaacaa aatcaatggc ttgatagttt gaagttttta
    22561 gttaagtgca ttgaagaaag tatgagaatc agtgatgaag cataaagaag catttaatgg
    22621 cgttgtcgtg ttaactgctg cattaattgt cattaaaatt ctgagtgctg tatatcgaat
    22681 tccatatcaa aatatattag gcgatacagg tttgtatgca tatcaacaag tgtatccaat
    22741 tgtagcatta ggaatgatat tatcgatgaa tgccattcct agtgcaatta cacaaaatat
    22801 agggaagtat catagtgacg aagcatatgc aaaagcagtc gcttatatac aattagttgg
    22861 tatattatta tttattgcta tttttgtgtt tgcgaacaat attgcacata tgatgggtga
    22921 tggccattta acaccaatga ttcaagctgc aagtttaagc tttatattta taggtatgct
    22981 tggcgtgtta agaggttatt atcaatctgc aaataatatg acagttccgg ctatttccca
    23041 ggttatagaa caagttatac gagtaggtat tatcattgtt actattgtta tttttgtaga
    23101 cagaggttgg acgatatatg aagcgggaac aattgctatt ttagcatcaa cgataggttt
    23161 tttaggttct tcaatttatt tagtagcgca ccgacctttt aagtttaaaa tggtaaataa
    23221 cactgcaaag atcgtttgga aacagttcgc actttcggtt ttgattttcg ctatcagtca
    23281 attaatcgta attttatggc aagtgattga tagtgttact attattaagt cacttcaagc
    23341 gatacgcgtg ccattcgatg ttgccataac tgaaaaagga gtctatgacc gtggtgcatc
    23401 atttattcag atgggattga ttgtaactac aacatttagt tttgcgctca ttcctctgtt
    23461 aagtgacgca attaaaatga ataatcaggt acttatgaat cgttatgcaa atgcgtcatt
    23521 aaagattacg attttaataa gtacagcagc aggaatagga ttaattaatt tattgccttt
    23581 aatgaacggt gtgtttttta agacgaatga tttaacctta acgttaagtg tttatatgat
    23641 tacggtcatt tgtgtatcgt taattatgat ggatatggca ttattacaag cgcaacatgc
    23701 tgtgagacct atttttgttg gtatgacggc aggattggtt attaaattta tacttaatat
    23761 cattttgatt cgtttaagtg gcattattgg tgcgagcatt agtactgttg tatcattaat
    23821 tatattcggt acgattatcc atattgctgt cacgagaaaa taccacttat atgcgatgag
    23881 acgatttttt atcaatgttg ttttaggtat ggtatttatg tcgattgttg ttcaatgcgt
    23941 gttaaacata gtgacaacac acggtagaat cactggactc attgaattat tatgtgcagc
    24001 agtattaggt atcattgcat tgtttttcta tatttttaga tttaatgttt tgacatataa
    24061 agagttaact tatttaccat ttggttcaaa gttgtatcaa attaagaaag gaagacgttg
    24121 atggcacata ccattacgat tgttggctta ggaaactatg gcattgatga tttgccgcta
    24181 gggatatata aatttttaaa gacacaagat aaagtttatg caagaacgtt agatcatcca
    24241 gttatagaat cattgcaaga tgaattaaca tttcagagtt ttgaccatgt ttatgaagca
    24301 cataaccaat ttgaagatgt ctatattgat attgtggcgc aattggttga agctgctaat
    24361 gaaaaagata ttgtctatgc ggttccgggt catcctagag ttgctgagac aactacagtg
    24421 aaattactgg ctttagcaaa ggacaatact gatatagatg tgaaagtttt aggtgggaaa
    24481 agctttattg atgatgtgtt tgaagcagtt aatgtagatc caaatgatgg cttcacactg
    24541 ttagatgcga catcattaca agaagtaaca cttaatgtta gaacgcatac attgattacg
    24601 caagtttata gtgcaatggt tgctgctaat ttgaaaatca ctttaatgga acgatatcct
    24661 gatgattacc ctgttcaaat tgtcactggt gcacgaagcg atggtgcgga taacgttgtg
    24721 acatgcccat tatatgaatt ggatcatgat gaaaatgcat tcaataattt gacgagtgta
    24781 ttcgtaccaa aaatcataac atcgacatat ttgtatcatg actttgattt tgcaacggaa
    24841 gtgattgata ctttagttga tgaagataaa ggttgtccat gggataaagt gcaaacgcat
    24901 gaaacgctaa agcgttattt acttgaagaa acatttgaat tgttcgaagc tattgacaat
    24961 gaagatgatt ggcatatgat tgaagaacta ggagatattt tattacaagt gttattgcat
    25021 actagtattg gtaaaaaaga agggtatatc gacattaaag aagtgattac aagtcttaat
    25081 gctaaaatga ttcgtagaca cccacacata tttggtgatg ccaatgctga aactatcgat
    25141 gacttaaaag aaatttggtc taaggcgaaa gatgctgaag gtaaacagcc aagagttata
    25201 tttgaaaaag tatttgcaga gcatttttta aatttatatg agaagacgaa ggataagtca
    25261 tttgatgagg ccgcgttaaa gcagtggcta gaaaaagggg agagtaatac atgagattag
    25321 ataaatattt aaaagtatca cggttaataa agcgacgtac gctagcaaaa gaagtaagtg
    25381 atcaaggtag aattacaata aatggtaatg ttgctaaagc tggatcggat gttaaagttg
    25441 aagatgtgct gacgattcgc tttggtcaaa aattagtaac agttaaagta actgcattaa
    25501 atgaacatgc atctaaagat aacgcgaagg gtatgtatga aatcattgaa gagcgtcgac
    25561 ttgaagaagc gtaaattgga ggtgacaagc aatgaaaaat aaagtagaac atatagaaaa
    25621 tcagtacacg tcgcaagaga acaagaaaaa acaacgtcaa aaaatgaaaa tgcgtgttgt
    25681 tcgtaggcgt attacagtat ttgcgggcgt attacttgcg ataattgttg ttttatcaat
    25741 cttgcttgtt gtccaaaaac atcgcaatga tattgatgca caggagcgaa aagcgaaaga
    25801 agcacagttt caaaagcaac aaaatgaaga aattgcgtta aaagaaaagt tgaataatct
    25861 gaatgacaaa gattacattg aaaaaattgc gcgtgatgat tattacttaa gcaacaaagg
    25921 tgaagtgatt tttaggttgc cagaagacaa agattcgtct agctcaaaat cttcgaaaaa
    25981 ataaatccaa attgattcaa aattatccga gtatagacat tgtgaaaaaa tccaaacaag
    26041 gatataataa gggaaaatcg aatcaaatcg ggaggattta tttaacatat gtcaatcgaa
    26101 gttggaaata agcttaaagg taaagtcact ggtattaaaa agtttggtgc attcgtagaa
    26161 ttacctgaag gaaaaagtgg tttagttcac attagtgaag tcgcagataa ttatgttgaa
    26221 aacgtagaag agcacctttc tgttggtgat gaagtagacg taaaagtatt atctattgct
    26281 gatgatggaa aaattagtct ttcaattaag aaagctaaag accgtccacg tagacaacat
    26341 acgagtaaac caagtcatca aaaaccagtg caaaaagccg aagattttga aaagaaatta
    26401 agcaatttct taaaagatag tgaagataaa ttaacttcaa tcaaacgtca aacagaatct
    26461 agacgcggtg gcaaaggttc aagacgttaa ttaaaataaa taaagactgc ttcgataagg
    26521 aatatattta gaatgatgcg tatcgaataa tcgattgcag cgttagacaa tctaagactg
    26581 tttcttaaat aaggagcagt ctcttttatt tgtaatgata taactaagac ttataccatt
    26641 tttgaaaatt gtaaaagtga ggtgatgtta tgcagttaaa tagtaatggt tggcatgttg
    26701 atgaccatat tgttgtcgct gtttctacag gtattgatag tatgtgttta ttgtatcaac
    26761 tactaaatga ttataaagat agttatagaa aactaacatg cttacatgtc aatcatggcg
    26821 ttaggtcagc ttcaattgag gaagccagat ttttagaaac atactgcgaa cgtcatcaca
    26881 tcgatttaca tatcaaaaag ttagatttgt cgcatagtct cgaccgaaat aacagcattc
    26941 agaatgaagc tcgaattaaa cgttacgaat ggtttgatga aatgatgaat gtattagaag
    27001 cggatgtatt gctaacggcg catcatttgg acgatcaatt agaaactatt atgtatcgta
    27061 tttttaatgg gaaatcaaca cgtaataaac taggatttga tgagttatcg aagcgaaaag
    27121 gttatcagat ttatcgacca cttttagctg tctctaaaaa agaaataaaa caattccaag
    27181 agagatatca tattccatat tttgaagatg aatctaataa agataacaaa tatgttagaa
    27241 atgatattcg taatagaatt attccagcta ttgatgaaaa taatcaactt aaagtatcgc
    27301 atttattaaa attaaaacaa tggcatgatg aacaatatga tattttgcaa tattcagcta
    27361 aacaatttat tcaagaattt gtgaagtttg atgaacagtc aaaatattta gaggtttcta
    27421 gacaagcttt taataactta ccaaactcat taaagatggt tgtgttggac tgcctattat
    27481 caaagtatta tgagttgttt aatattagtg ctaaaacata cgaagagtgg tttaaacaat
    27541 ttagtagtaa gaaagcacaa ttcagtatta atctcacgga taaatggata attcaaatcg
    27601 catatggtaa attaataata atggctaaaa ataatggcga tacatatttt agagttcaaa
    27661 caattaaaaa gccaggtaat tatattttta acaaatatcg attagagata cattctaatt
    27721 taccaaaatg tttatttccg cttacagtga gaacacgaca aagtggcgat acatttaaac
    27781 tgaatgggcg cgatggttat aagaaagtga atcgcctgtt tatagattgt aaagtgccac
    27841 agtgggttcg ggatcaaatg ccaatcgtat tggataaaca acagcgcatt attgcggtag
    27901 gagatttata tcaacaacaa acaataaaaa aatggattat aattagtaaa aatggagatg
    27961 aatagcgtta tgcataatga tttgaaagaa gtattgttaa ctgaagaaga tattcaaaat
    28021 atctgtaagg aattgggagc acaattaaca aaggattatc aaggtaaacc attagtatgc
    28081 gtgggtatct taaaaggctc agcaatgttt atgtcagatt taattaaacg aattgatacc
    28141 catttatcaa ttgatttcat ggatgtttct agttatcacg gaggcactga gtcaactggt
    28201 gaagttcaaa tcattaaaga tttaggttct tctattgaaa ataaagacgt attaattatt
    28261 gaagatatct tagagactgg tactacactt aagtcaatta ctgaattatt acaatctaga
    28321 aaagttaatt cattagaaat agttacttta ttagataaac caaaccgtcg taaagcggac
    28381 attgaagcta agtatgtagg taaaaaaata ccagatgaat ttgttgttgg ttacggttta
    28441 gattatcgtg aattataccg aaacttacca tatatcggta cgttaaaacc tgaagtgtat
    28501 tcaaattaat tttttaatca atttcagtta ttattactat gcgtttgaga aataatagtg
    28561 tagactcaaa
//
LOCUS       DALAGF010000023        28044 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.023, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000023 DALAGF010000000
VERSION     DALAGF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28044
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.023"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(391..837)
                     /gene="perR"
                     /locus_tag="O2692_000963"
     CDS             complement(391..837)
                     /gene="perR"
                     /locus_tag="O2692_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HCZ4298601.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
     gene            complement(934..1884)
                     /locus_tag="O2692_000964"
     CDS             complement(934..1884)
                     /locus_tag="O2692_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCZ4298602.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLRRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            complement(1890..2345)
                     /locus_tag="O2692_000965"
     CDS             complement(1890..2345)
                     /locus_tag="O2692_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCZ4298603.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGYSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2426..3715
                     /locus_tag="O2692_000966"
     CDS             2426..3715
                     /locus_tag="O2692_000966"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCZ4298604.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            4008..5102
                     /locus_tag="O2692_000967"
     CDS             4008..5102
                     /locus_tag="O2692_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCZ4298605.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(5192..5284)
                     /locus_tag="O2692_000968"
     CDS             complement(5192..5284)
                     /locus_tag="O2692_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298606.1"
                     /translation="MFDFSTILKRKFVTGWWGHNIEKAVRDFLN"
     gene            complement(5293..7029)
                     /locus_tag="O2692_000969"
     CDS             complement(5293..7029)
                     /locus_tag="O2692_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HCZ4298607.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(7320..7862)
                     /locus_tag="O2692_000970"
     CDS             complement(7320..7862)
                     /locus_tag="O2692_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCZ4298608.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKERYKKIRQY"
     gene            complement(8164..9201)
                     /gene="mutY"
                     /locus_tag="O2692_000971"
     CDS             complement(8164..9201)
                     /gene="mutY"
                     /locus_tag="O2692_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCZ4298609.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
     gene            9353..10330
                     /locus_tag="O2692_000972"
     CDS             9353..10330
                     /locus_tag="O2692_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCZ4298610.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            complement(10591..11427)
                     /locus_tag="O2692_000973"
     CDS             complement(10591..11427)
                     /locus_tag="O2692_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298611.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIVLIVFAVVMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCFLLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(11436..12953)
                     /locus_tag="O2692_000974"
     CDS             complement(11436..12953)
                     /locus_tag="O2692_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298612.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            complement(12968..13282)
                     /locus_tag="O2692_000975"
     CDS             complement(12968..13282)
                     /locus_tag="O2692_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HCZ4298613.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            complement(13260..14078)
                     /gene="recX"
                     /locus_tag="O2692_000976"
     CDS             complement(13260..14078)
                     /gene="recX"
                     /locus_tag="O2692_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCZ4298614.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            complement(14339..15148)
                     /gene="sgtB"
                     /locus_tag="O2692_000977"
     CDS             complement(14339..15148)
                     /gene="sgtB"
                     /locus_tag="O2692_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HCZ4298615.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            complement(15489..16004)
                     /locus_tag="O2692_000978"
     CDS             complement(15489..16004)
                     /locus_tag="O2692_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCZ4298616.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            complement(16135..16296)
                     /locus_tag="O2692_000979"
     CDS             complement(16135..16296)
                     /locus_tag="O2692_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298617.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            16520..17671
                     /gene="yfkAB"
                     /locus_tag="O2692_000980"
     CDS             16520..17671
                     /gene="yfkAB"
                     /locus_tag="O2692_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HCZ4298618.1"
                     /translation="MNSVKLKQPVSIYNDPWEAYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRNAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            complement(17931..18461)
                     /locus_tag="O2692_000981"
     CDS             complement(17931..18461)
                     /locus_tag="O2692_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCZ4298619.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(18551..19807)
                     /locus_tag="O2692_000982"
     CDS             complement(18551..19807)
                     /locus_tag="O2692_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCZ4298620.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAGKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            complement(19810..20010)
                     /locus_tag="O2692_000983"
     CDS             complement(19810..20010)
                     /locus_tag="O2692_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HCZ4298621.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHE"
     gene            20157..20621
                     /locus_tag="O2692_000984"
     CDS             20157..20621
                     /locus_tag="O2692_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCZ4298622.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            20628..20903
                     /locus_tag="O2692_000985"
     CDS             20628..20903
                     /locus_tag="O2692_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCZ4298623.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            21181..22398
                     /locus_tag="O2692_000986"
     CDS             21181..22398
                     /locus_tag="O2692_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCZ4298624.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
     gene            complement(22514..23143)
                     /gene="vraR"
                     /locus_tag="O2692_000987"
     CDS             complement(22514..23143)
                     /gene="vraR"
                     /locus_tag="O2692_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HCZ4298625.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMDDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(23133..24176)
                     /locus_tag="O2692_000988"
     CDS             complement(23133..24176)
                     /locus_tag="O2692_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCZ4298626.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            complement(24173..24874)
                     /locus_tag="O2692_000989"
     CDS             complement(24173..24874)
                     /locus_tag="O2692_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCZ4298627.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            complement(24889..25275)
                     /locus_tag="O2692_000990"
     CDS             complement(24889..25275)
                     /locus_tag="O2692_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298628.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            complement(25492..26250)
                     /gene="map"
                     /locus_tag="O2692_000991"
     CDS             complement(25492..26250)
                     /gene="map"
                     /locus_tag="O2692_000991"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCZ4298629.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            26778..27764
                     /locus_tag="O2692_000992"
     CDS             26778..27764
                     /locus_tag="O2692_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCZ4298630.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
BASE COUNT         9117 a         4835 c         4001 g        10091 t
ORIGIN      
        1 ttctagtaga gtcaacataa aacaacactt atttttgttt aaaaattact gttgaaggga
       61 ttattttcta ttcgtaagta ctatatgtat ttaaagtgta attccttatg ctttttactt
      121 cagttttaca aacaaactta tactcacttt atggatagtt atttagtaga ccttcgtttg
      181 aatattttta tgtttattta taaaggatac tagttattag ttaataccac ttccgtagtt
      241 agaatattct tttcttcaat ttaaatattt ttcagaataa gcataaaaaa aagttaaaca
      301 actttcatgt cgtttaactt cttaaatatt acgcttactt atatgaagta agctaacgct
      361 tttttaattt gtcatactac caaagttaaa ttatttatct tggcattctt tacaaactcc
      421 ataaatttcc attcgatgat gtgttacgtc aaagtcagtc atatgctgag ctaatctttc
      481 aatttcattt aactgtggat attgaaaatc aacaatctta ccacattgtt cacatataat
      541 atgataatga ttatgtgtat taaagtcgaa tcgacttgat gagtctccat atgttaattc
      601 ttttacaatt ccaatatctt taaacactct taagttatta tatattgtcg caacacttat
      661 atttggaaaa tcaggtgaaa gtgcttgata aatttcatca gctgttggat gagtatgtga
      721 agaaattaaa taacgtaata ttgcttgtct ttgaggtgta attcttacgc ctgcttgtcg
      781 caatgatgca attgattctt ctagttcatg ttcaattgat tctatttcaa cactcatcta
      841 tatcaccatc tttcttatat aataattatt attacttact ataaatataa ctgtagtatt
      901 gcgcgcgtgt caatatttac atgatgattt catttaatag ccttttttag catcaacttc
      961 attctcaatt agaccattct tattgagaaa attaactaga ttgtttttaa aaatatctaa
     1021 taagtcatac tttgcttcat aatcattacc agttatatgc gctgttatag ttacattatc
     1081 caattcatat aattcatgat taggtttcaa aggttcattt tcaaacacat ctaaatatgc
     1141 atgtcgaata actttacttt ttaatacttc tattaagagc gcttctttaa ctatgctacc
     1201 tcgtcctata tttataaaaa gtgcttcatc tttcattaat tcaaaatgtt ttttctttag
     1261 taaatgaatc gtttcttgcg tttctggtaa agcatttata ataatgtcag catttggtaa
     1321 tgtgctttca agtgattcga tagtatatat ctcgtcaaac tcatctttgt tctgacctga
     1381 cttgctcaga ccaattaaat tcatattaaa agcctttgct aacttcgcag ttctagtagc
     1441 aattgcacct gtacctaaaa ataaaactgt ttgtcctgat aggcgtttac cagttatttt
     1501 cgaatcatat atatgttgtc gttggttatc atatgatagt ttcatctttt tataatcatc
     1561 taaaatgaaa gctaaaatgt attcagataa ttgtttagct tgaacacctt ttccattagt
     1621 taaaagtatg ccgtgatctg caatataatc taacggcaat gtatttacac ccgttgcaaa
     1681 ccatgcaatc cattttaaat tcgggcaacg tcgtaaaaat gcctcattga taccgccatc
     1741 ataaccaact aatatatcta aatcagccaa gtcgctctca ggtatttctg atgctttttt
     1801 ataaaattta aaatctaaat ctgaaaagcg ttgttgtaat tcagtttcaa cttcacgcat
     1861 acgatttaac ccaacaactt tcatatatgt caccccaaaa tgtttttaag ttcttctatt
     1921 tgtgttttaa ccttaacctt ctcgataaca tctaatactt taccttgttc atctattata
     1981 aaagtcgttc ttacaatgcc catactttct ttgccaaatg atttttttaa ctgatatacg
     2041 ccagtttctt tagctaattt aaaatcttca tctactaata aatcgaaatt caatccgtgt
     2101 ttctcaataa aattttggtg ttttttcttt gaataaccgc ttataccata tactgcaaca
     2161 tctaaatcat tgaacatttc taaattgtct ctaaagtcac aagcttctgt ggtacaagta
     2221 ggtgtattat ctctaggata aaaatatata atcgcctttt taccttttaa tgtatcattt
     2281 gtaatgacag ttccgtcttg attttctaat ttaaatattg gaaattgttc tcctttttgc
     2341 aacatataat cacctttttc ttcattataa taattttatg atacgatatt agatgaaaat
     2401 attaaattaa aagaggttga tataaatgaa ttttagtgaa agtgaacgtt tacaacaact
     2461 ttcaaacgaa tatattctag gcggtgtcaa ttccccttct cgttcttata aagctgtagg
     2521 aggcggtgca cctgttgtta tgaaagaagg acacggtgca tatttatatg atgtcgatgg
     2581 caataaattt attgattacc ttcaagcata cggtccaatt attacggggc atgcacatcc
     2641 tcatattact aaagcaattc aagaacaagc tgctaaaggt gttttatttg gtacaccgac
     2701 tgaattagaa attgaattca gcaaaaaatt acgtgatgca attccatctc ttgagaaaat
     2761 tcgctttgta aattctggaa cagaagcagt catgacaaca attcgtgttg cacgtgcata
     2821 tactaaaaga aataaaatta taaaatttgc tggatcttat catggccatt ctgatttagt
     2881 attggttgca gcaggtagcg gcccatctca gctcggttct ccagactcag ctggtgttcc
     2941 agaaagcgtc gcacgtgaag tcattactgt acctttcaat gatattaacg cctataaaga
     3001 agcaattgaa ttttggggtg atgaaattgc cgcagtatta gtagaaccaa ttgttggtaa
     3061 ctttggaatg gtaatgcctc aacctggatt tttagaagag gttaatgaaa tttcacataa
     3121 caatgggacc ctagtgattt atgatgaagt aattactgca ttccgtttcc attacggtgc
     3181 cgctcaagat ttattaggtg ttatccctga tttaactgca tttggtaaaa ttgttggcgg
     3241 tggtttacca attggaggct atggtggacg tcaagatatt atggaacaag tagcacctct
     3301 aggacctgca tatcaagctg gtacaatggc tggtaacccg ttatctatga aagcaggtat
     3361 tgcattactc gaagtactag aacaagacgg tgtttatgaa aaattagaca gcttaggcca
     3421 acaactagaa gaaggtttac ttaaattaat cgaaaaacat aatatcacag ctacaattaa
     3481 tcgtatttat ggatctttaa cattgtactt tacagatgaa aaagtcacac attatgatca
     3541 agttgaacat tctgacggcg aagcgttcgg taaatttttc aaattaatgt taaatcaagg
     3601 tatcaattta gcaccttcta agtttgaagc ttggttctta acaactgaac atacagaaga
     3661 agatattaaa caaactttaa aagctgcaga ctatgctttt agtcaaatga aataactttt
     3721 aaggaagtat atttttaatt gaattaaaat aaagcaaatg ctcacatagt agattcattt
     3781 tatatcgtta actattaacg tttaacttga tatgtcacta tgtgagtttt tattttacaa
     3841 atttatacta ttttaatctc taactaaagt tttacatcgc tatcagtatt atgtatgatt
     3901 tattttaaca actataaata agcttgaatt ttgtaactag taagtgtata acagtaatga
     3961 atacgaatat tagatattca ataaacttaa aaggggcgtc attatttttg agactaggag
     4021 ctcggatttt taaaactggt atagccatta ttttagctat gtctatcgct tctttactac
     4081 cggatgatgt tggtctgaaa gccttagctg gtgtcagtgc tgttgttgca atgcaaccta
     4141 gtatttatcg ttcatttaaa acagtttctg atcaagcatt gggtaacatt ataggtgcca
     4201 tactatctgt tacaatggta acaattttta gtgataattt tatcattatg ggtgttaccg
     4261 tcatagtact tattgctatt ttatttaaat ttaatttagc acatgttgct actttggcga
     4321 gtgtaacagc attaatcatt atgggacaac ataccggttc tttctatatt accgcctttt
     4381 acaggtttgt acttgtaatg attggtgtca taagttcatc tctagttaac tttgtgtttc
     4441 taccacctaa atttgaaaca aaaatttact ataattcatt aaatatatcc tcagatattt
     4501 ttatgtggtt taaattagta ttaaatgata cgacggagtt taataatatt aaacaagata
     4561 gtcataattt aaaacaacgt gtcgaaaagt tagagaaaat ttatgattac tatagcgaag
     4621 aacgtcctat tacaaaaaaa catattcacc aacagaatag aaagaaaata cttttcagag
     4681 aagtagttca gacgactaga caagcttatg aagtattaaa caaattgtcg cgttatcaaa
     4741 acgacttata tttacttaat aacaatttcc tattacaaat caaattagat ttagattcat
     4801 taactgcttt tcacgagcaa attttagcta gcctttctaa aaaagcacgt tataatgtga
     4861 cacatgttga ttatgagtta gataatccgc agaaaaaaga tttattgtcc acatttcagc
     4921 atgagttaat aaatcatcct tatcaaacgg aatattcgtt tgccaatgtt atgcaaattg
     4981 ttgcagccat cgaagagtat cgacaccatt tagagcattt agaccgcata agaattagct
     5041 ttttcacata tcatagatct gatgcagaca ttgaaattgt tgaagaagac tttgatttat
     5101 aacattaaaa caattttatc aattaatgcc acaatgagat aaactaatat tgaatctcga
     5161 aattaaattt aaaaaagcag taagattatt ttcaattaag aaaatctctt actgcttttt
     5221 ctatgttatg accccaccat cctgttacaa attttctctt caagattgta ctgaagtcaa
     5281 acaactaata ctttataagt tttgaatgct atataaatgc tcgtaagcac cttgttttgc
     5341 aatcaattca cgatgcgtac ctgtttcaac aatatgtcca ttttcaatta cgacaatttt
     5401 gtcagcatgt gtaatagtgg acaagcgatg cgctacgata agtgtcgttc gatctttact
     5461 caacacatct aatgcttctt gaataatgga ttcactttct aaatcaagtg cacttgttgc
     5521 ttcatccaag ataagaattg gcggattatt taaaaatatt ctagcaatcg ataatctttg
     5581 tttttgacca cctgataatt taacacctcg ttcacctact tcagtgtcat atccctgtgg
     5641 caagttcata ataaagtcat gtgcattagc cattttcgcc gcttcaacta cttcttcatc
     5701 tgttgctgtt ggacgaccaa gtaaaatatt ttccttaact gtatcagaga ataaaatatt
     5761 atcctgttgc accaatccta tttgatttct taaacttccc gttaaaaaat ctttaatgtt
     5821 gtgaccatct attaaaattt gcccagaagt tacatcgtaa aatctcggta ttaagttaat
     5881 taatgttgat ttaccaccac cactcatacc tacgaaagca actgtttctc ctttttcaat
     5941 actcaaatta atatctttta aaattggagc ttcgttatcg ttatattgaa aactaacatg
     6001 atcaatatca atacgacctt gtttaatttc aataggttga gcaccaacac catttttgat
     6061 gtcataatct tcatcaatta attggaatac acggtccatt gaagcaaaac tttgcgttaa
     6121 agttgtaaat gatgcgacta aacgacgtaa aggcccgaac aataactcta agtatccaac
     6181 aaatgctgca agtgtaccta ctgtgattga tccagaaata gcaagatatg caccaacacc
     6241 gatgacaata attggtccaa tatctgtaac tgtattaatt gcggcaaagg aataggcatt
     6301 ccatcttgta tgtttcaacg cacgtgttag gaaattagta ttctttttat caaagttttt
     6361 cgcttcattg tcttcaatcg caaaactttt aacgactgaa ataccttgaa cacgttcatg
     6421 caagaatcct tgaacctcag ctaatgcttg agatctttca cgtgtcaatt ttcttaatct
     6481 tccaaagaaa acgtacaccg ttaaaatgta aaatgggaag ataaacagtg ctgctaaagt
     6541 caatttcaca tctaaaaaga acattatgga tagtgcaata ataattgtta tacaatctaa
     6601 ccaaatattc attaacccgg ttaaaatgaa atcttttgtt tgttcaacat cattaatcac
     6661 tctagatatt acttgaccta cttgattatt agcataaaat ctcgcactta aagcttgtaa
     6721 atggttgtat aactttttac gtatatcata caatatttta ttacttgtcc attgcgccaa
     6781 atattgacgt ataaattcaa ttggtggtct cactattaca aaaataaata atgcgatacc
     6841 aatggcaata gttaaatgat gaactttttc atcagtcgtt agtgcgtggt tattaatcac
     6901 gccatctatt gcatatttaa ttaatagtgg tataagcatt ggtataccaa acttaattat
     6961 cccaacaata atcgttgcaa aaatacgata tttgtatggc ttaacaaatt gcaaatatcg
     7021 tttaatcata caatcccccc taatctattg ccctatccta ttcataagca taaaaatgaa
     7081 tagaggttgg atacataatt tgtagatgta aattcttctt acaatttaca tttttaaaat
     7141 taagatatct caacctctgt catattcatg tgattaaata cgttgtctta ttcgtatgtt
     7201 atattctatt caatttaatc tatggatact gtgtccccac acgacagcaa aagttatcat
     7261 acttctttac atcactaagt caatataaat gatttaatca gtatttacac tttatttgct
     7321 taatactgtc taattttttt gtaacgttct ttccaaactt tgataaaatc tggcgcgaat
     7381 gggcccttct tctgttctat ccattgttga agaatgtcca cgttgcgtct taaaataata
     7441 tcaatatcat gcggataatt catttgattc atatgttgct catattcatc ttcatctaat
     7501 aaatgatact ttccgtttgg atatacttta atatctaaat catagtctat atattttaat
     7561 gcctcttcat cacaaacaaa tggtgatgac aaattgcaat agtaataaat tccatcttct
     7621 ctaaacatgc agataacatt aaaccaatat tctgagtgaa agtaaacaat tgccggttca
     7681 cgtgttatcc aagttcttcc gtcactttca gtcactaacg tatgatcatt tccaccaatg
     7741 acaacatgat cagtaccctt taatattgtt gtttcagacc aaacgcgatg aatcttacca
     7801 tcatgtttat aactctgaat tttaatgttt tccccttctt taggtatgga ttctctgacc
     7861 atactccaca ccaccttctg ttaatttaac cattataaat tatagcatat ttcagaaata
     7921 gtattatata aatacatatt tttacgaaat aagattttac tacttaataa ttaaactcgg
     7981 taatattgct aagtactaca acagagattt acatgtccca tttaaagtat ataaaatcat
     8041 cacttttata tatcaacact ttaacttttt gacattgtta ttctatgaga tttaaagata
     8101 tcatttatac tttttaaaat taatgtcact atgttttccg ataatattac caatcatcga
     8161 atgttaccca tttataaatt gataaatctt tgacataggt acagggaatg tatattgatc
     8221 tcgatcactt aaatcaaacc aaatcatgtc atctggtaat gtttcaatgt taattgctcc
     8281 tgaaacggcg tatactttaa tcttccatgt taaatgagta aattgatgct tcaactcaaa
     8341 aataggtgtt tctactggtt gaatgtcatg accgattttt tcagtcattt tacgtctagc
     8401 atgctcactt tcaaacatag gaaattgcca cataccatgc aataattttt cgctacgctt
     8461 ttgcaacaga tattgacctt gattatttct aattaaaaag acggattgct caattacttt
     8521 tttacttaca tttttagatt taacaggtaa cttttcaaat gtacctttat caaatgcctc
     8581 acagttttct tgaactggac aaaataagca taatggattt tttggtgtac aaattaacgc
     8641 ccctaattcc atcatagctt gattaaacgt tccagcttct gtagtaacat acggtaataa
     8701 ttcttgttcg tacgatttcc tcgtcgattg taatttaata tctcgatagt catcattcaa
     8761 tctagaccat actcgaaaaa catttccgtc tacagttgct agtggtacat tatatgcaat
     8821 gctcattact gcagcttgtg tgtatgggcc aacacctttt aacgctttaa attgatcagg
     8881 atctttggga actaagcctt catatttatc atgaacttct ttaatcgccg tatgaaaatt
     8941 tcgagctcta ctataatatc ctaagccttc ccaatacttt aacacttcat cttccgaagc
     9001 ttgactcaaa acttccacag ttggaaatcg ttcaacaaaa cgatgataat agtcaataac
     9061 tgttttaact tgtgtctgtt gtaacatgac ctcacttaac caaatatagt acggattggt
     9121 cgtttgtcgc catggcattt ctctttgatt ttcatcaaac cagtgtatca aattttcttt
     9181 aaaactagac tgctgataca tttataaaac cctttcctca ccaaaattaa ttgtctttac
     9241 tcataatgtt tttattgtac attaaaatca tggttagtat gtaagttaat ttagttattt
     9301 gcgaaattgg attataatag tatatataat attatgaaat gagtgaactg atatggacac
     9361 tgcaacacat atcgcaattg gggtgggcct tacagcactt gcaactcaag atccagcaat
     9421 ggcttctacg tttggtgcaa cagctacaac ccttatcgtt ggttcattaa ttcctgatgg
     9481 ggatactgtt cttaaattaa aggacaatgc aacatatatt tcgcatcata gaggtatcac
     9541 gcattccatc cctttcacaa tactatggcc aattttaatt acatttttaa tattcacgtt
     9601 ctttagtgga accaacccat ttcatgtatg gatgtgggct cagctcgcag tatttttaca
     9661 tgtctttgta gatatattca attcttatgg tacacaagcg cttagaccta tcacaaacaa
     9721 atggattcaa ttaagtgtga ttaacacatt tgaccctatt attttcacag ttctttgtat
     9781 tggtattgta ttatgggtta taggcttgca tccatttgca gtcttctttc ctataatcgc
     9841 tttactaatc atttattaca tgattcgttt taaaatgaga gccgtaatta agcaacaagc
     9901 tttaaaagca attcaacaag agcatcaccc tgttaaagta tttgttgcgc caacaataaa
     9961 atttatggaa tggcgtgtcg cgatacaaac tgatgcacat gactatgttg gaaaagcata
    10021 tggtaggaat gtggtgttta gtgataaagt ggaacgtcaa acattatcaa cagactccat
    10081 tttatggaaa gtcaaaggta ataaagatat acgtactttt ttaaactttt catcaatcta
    10141 tcgttggcaa acaacaacgt tagcagatgg ttctactgaa attcgtttga ttgatttgcg
    10201 ttatttaaaa aatgatcatt attcatttgt ggcaattgca catgtaacaa acgataatgt
    10261 catagaccac tcttatattg gctgggtatt tacagaagat aagttacaac gtaaactgta
    10321 tgctaaataa tttcaagtta ttattcacta aagttaatct ataaaaaatg aacaaccggg
    10381 cagaatgaaa atcaaaacga tttttactct gtccggtttt ttaatgtaaa actatgaatg
    10441 cttttacaaa atctaaaatt tatattgttg ctaacaaact acctttaatg actcgaaata
    10501 tcaaaatcag tataggaaaa caatatctag atgatattct aattgtttct gattctcaca
    10561 gattaattta cacaacaggt cagctaaaca tcatgaagaa gtatccgcct cgtctgtact
    10621 atcatttgaa acatcctgtt gattatcagt ttgtgtcact ttagatgttt tattataaat
    10681 tgcgtgtgtc gtataccttg ctaatacgaa atttactgca gctattaaac ataagaataa
    10741 aataaagtaa aaatgatatg gaatgttttc catacttgat ataccaaata tgatagattg
    10801 tgctatacca ttaacaatgt aatacattgg atttagcatt aggatgtgat tgataaatac
    10861 atgatttgga tttggtatga aaataattgg taacaataag aaacacaata cacaaacccc
    10921 ataaaatatg atatttattt tttcagttaa cagtcgaata agaccaaaag taacggatat
    10981 taatcctaca aaaatagttg ccatcacaat aaaatagaat agcgctatat atgatgtttc
    11041 gaagtttact ggtttaacca atgcactaat catcgtcaaa atgactagca taataaaact
    11101 taaaatagac ataataacta ctggcgtcgc tgcggaaata ttaaataact tagtaactaa
    11161 aaagtcattc atataatgat gataagagat ataaattgtg aacatcacta ccgcaaaaac
    11221 aatcaataca atgattctaa ataaccatct cacctcttta acatcggtca aatgactaaa
    11281 ggaaaatagc gtttcacttg caataataag tattaaacat gcagctaata cgattagaaa
    11341 acttttccaa ctttgtttta aacgttctgt tacatacctt ttagtatgcg gtaagttttt
    11401 aaaatataaa ataatattat ctagcatctt tacacctaca attcaatata aatccatttg
    11461 tcttctttca aattggcaat acaatatcca ttcccaactt tagtaatcca aatgttattg
    11521 ttaatattaa acttatcttt taattctttt ttatctacaa ttggataaac aaaaccgtat
    11581 aactgattat tatcattgaa aagtaattgt atcggttgtt gtgtaacgga tgcaacgaaa
    11641 cggttatcat catcaggaac taaagttttt ataactgaac tatgcttttt atgtaattga
    11701 ctattaaagt atgcatagta attactataa tttgatttca tatatggtgc taatttttta
    11761 aaactatgcg tttgatataa acctgccggg ttaaaatatt ctaatttatc tttactaaca
    11821 tagtaagtct tattatcggc ggtaacacga taatttttac tattttcgcc agtaattgta
    11881 actacactat attttggaat tgtgacttgt ttatcccctt gcatatccac actgccatta
    11941 aaagcaattc cataagctaa cttttcgcca tatggatctt tattttcatt ttgcatcgta
    12001 gcacgattcg taaaatctgt tatcgaaata attcctatat tattgaaaat gagcaacatc
    12061 atcaatgcta agcctaaaat agtacctgag gccaaagtcc aaaaacgcac taaaaactta
    12121 ggcggttttg catgattata gcgctcaaca cgtttgaaat tataggtcat ctctggtatt
    12181 cttgtacgat tctttttcca atctaaatca aagttttcaa tttcttcttt tgagtttaga
    12241 cttaatcgat cacgttcatg ttctttaaaa gatggaataa cttgtttaag tgacccttcc
    12301 attcttaatt gaccatgtga aaaccaagct atgtagttac tcacttgtga aattttatca
    12361 atatcatcac caattgacac aatcgttaaa ttattttcaa tataatcatt tgttaattca
    12421 atcgcacgtt ccataaattg tggtgttaaa tagtcaataa catgatttaa aataataata
    12481 tttgattttg atgagcgtgc aatacttaat agtaattgag cgtatgccgc tttcgaaata
    12541 tggttaaccg gcttcgtttt ataatcacct aaatgtgcat attgaataat ctgttcagct
    12601 ttatgatcat taatttcata tggaaatagt tgaactaact gcgctgtata agcttcaaca
    12661 gtttgatgaa ttaacgattg atcttcaata tatccgtaga acaaatcttc agtacaaact
    12721 actttacctt tatcaggttt aattgcacct gccaacaatt gacctaccaa cgctttggaa
    12781 gattcaggtt caccaattat acctaatgct tctccttgat aaatatgtaa actaatattg
    12841 tttaaatcga tatcttcagc atcatatcca aaaggtaaat accatttctt attctgttta
    12901 ttcctatagt agtgtgttac ttttagtaac tttaaaacaa ttgaacttcc catctatttt
    12961 catccttcta taattgtaat aacgctatag gtaatccttc ttcgggttca tcgctattaa
    13021 atctaaaccc ccacgcaaaa atacctttta atctttctac tttaaaatat tgattactgc
    13081 catcagtcag tttataaatt ttaccaattt ctatcttttt tctgtctact aagtaacttt
    13141 cggcaacaat tactttactt tgataaacgt catattcatt taaaatacca ttcatctcag
    13201 cttttctcat tttctcttta taaagttgta tttcgtgtct taattctggc tcagacatct
    13261 cactcaattt cttctgttcc atcggcaata ccactttctt ctaatttagc tttaatttta
    13321 tcatatttat atccttttct cataaggcct tcgatagttt ttgaaattaa tttctgttgc
    13381 gtgtacttct ttcgattttt attataaatt ttttctaaat ctcgttgtaa taaatcgtct
    13441 aaaaccgctt catcttgtgt aaaatccatt tcattcaata cagcatgaat ggtttccatt
    13501 tcaaaccctt tttgaattaa agattgcatt actttttctt taactttatt ttgtggccct
    13561 ttttttgttt tagatatttt ttcagcaatt tggataatat catcaagttc ctgttgttct
    13621 ctataaagtt ctgtaaatat ttcaatgata tttggttcta taccaagttg atatagtttt
    13681 tgttgataaa ttttaggtcc tttatctgtc gtgcgaatca ttgtattttt taaactttcc
    13741 gcataatctt gatggtcgat taacttttcg cgataacaat attcaatcac ttcagaaatc
    13801 gcttgctctg atatctcttc tttttgtaaa tattgtataa cttctttttc agttctcttt
    13861 ttatatgata aatattggat tgctttattt aaacctatgc gataatgatc atacttttga
    13921 atctctgcca tgtcagcagc ttcaagttgt tgcccttttt ttaaattaaa tttgactaat
    13981 gtatcaatat ctatacccat ttcaaattgt tcgtctaaaa aaagattaaa ccgttctttg
    14041 tttttctttt gaacttctat tttagtaatc ttcggcactg gatcaccccg ttactttctt
    14101 aagttttaat acaatactct tttattattc aataagccac ttcctatagc aaatgtttaa
    14161 ctttaaatat ttttcgatgc taacaaaaaa tcacactatc atcttttaaa atgaaagtgt
    14221 gattacaagc aaatctgtaa aatttataaa gcagaaacaa ttcaacttta tcattatgac
    14281 atttcaatta aaccttctac attatagttc caagcatctt acacatgaat gcaagtattt
    14341 aacgatttaa ttgtgacata gcctgttgat attgtgtttc attgatataa ttttgttgct
    14401 tcattttttc taagttcgtg cttacacgtt gcgtaaaatt ctctgacata ttattgatat
    14461 tatatacgct aggtgcattg actttactag ctaaaatagc gctttgtaaa actgttatgt
    14521 gagacattgt tgtactattt ttattcacgg ttgttccaaa gtaatggttt gctgcgccct
    14581 caagcgtata ttgattatcc ccaaagtaaa tattatttaa ataaaagctt aaaatttcgt
    14641 tcttattata ttgtttttca actcgatgag ctacaaataa ttcttttact tttctagtaa
    14701 atgaacgatc attatcataa aaataatttt tgacaacttg ttgtgtaatg gtactaccac
    14761 cttgcacatc tctgtcgcta atcgttgaaa ataaagctct agttgtacct ttcaaatcga
    14821 atccatgatg attgtagaat cgttcatctt ccattgaaat aaaggcacct ttaacatact
    14881 ctggcatgtt atcagctgac acaaaactac ttttattttc aatttttctt agttcatcca
    14941 cattatcgcg tgtagataaa aaatacatga taccaataaa caatgcgata atgattagaa
    15001 tggttaataa tatttttaat agtattcgtt tacttttttt ctttttcggc ggtttgccaa
    15061 ctggttgata atacgtatta tagtgaggtt cgtgtttcat atgctcaaaa tgttcatttg
    15121 agtttgagta cctatcgctt cttttcatgc gtttgctcct tcttttaaaa ctcacttagt
    15181 atataccttg agtttaccag tactatcaca aataggctac actttttggg aaaatcagtc
    15241 caagggctta caatcgtata cgccatcata cttacttttt tgttttttga aaaaattata
    15301 gataaatcat tgcaatttta aatattaatc atgtcaaata ttgttatatt ttataaaaat
    15361 aaaagaccat ccctattaaa tgccaataga gacgaccttt tatttgttat tcatttatta
    15421 aaactaaaat ccatatttca tttcaaacga aaatatataa attttaacaa tcgataacca
    15481 caatacttct attgtaattg tttaacgatt tctcgattaa aatcatctaa atcgtctggt
    15541 actcgacttg ttacaatatt gttgtctaca actactgact catcaactac atgtgcgcct
    15601 gcatttgata aatctttgcg tacatttaat actgctgtta acgtacgacc ttttaaatcg
    15661 tctgtatcta ttagtatttg tggcccatga caaatggcaa atgttggtac atcattttta
    15721 gtaaagtatt tagcaaatgt gccatatcga ccttctgtat ctccacgtaa atgatctggt
    15781 gaaaatcctc caggaattaa taatgcatca taatcttctg gtttagcttc tgcaatgcct
    15841 acatcgacag taactttttc accgtgttta ccaacaactt cactatttgc agtatctcca
    15901 atcactacag tattaaagcc tgcattctct aatgcctctt tagggcttga atattctata
    15961 tcttcaaatt cgtttgctag aataattgct acttttttag tcattgcaaa tcacctttct
    16021 atatatcatt gatataatta ctatagacaa gtaaatcagt gattaaacat acaagatata
    16081 aaaaatatta agcgactgtc gcgatatcta accctaacac atcttatgtg gcatttactt
    16141 agatactaat ttaacctttt cttcaagctg atctaacaat ccaatccatt catctatatc
    16201 ttcaacacgt acttcatcag gatttacatg atcgatatcc tcaataaact tatttaaacg
    16261 cgcttttatc tgttcgattg tttgttgttc attcataaaa agttaactcc ttttattttg
    16321 ttttcttttt cattattatc ctaacagaaa ttgcgttaaa gcgatataat cttagctata
    16381 tttatgacat tcaaattatt ttgactttta aaaatcccct tttcaattaa ctaaaattaa
    16441 gagataattt gttacgagtg ataatacgaa gtggtatcat accgatatga accaaataga
    16501 aagaaggaag tttaagacga tgaatagcgt caaattgaag caacctgtta gcatttacaa
    16561 tgatccatgg gaagcatata acgatgttaa agaacatggc caattaactt taagtaacat
    16621 cgaatttaca actacaaatc tttgtaatat gcgttgtagc cactgtgcag ttggttatac
    16681 tttacaaact gtcgaccccg agcctttaga tatggactta atttatcgta gacttgatga
    16741 aattccaaat ctgcgaacga tgtctattac aggtggcgaa ccaatgtttt ctaaaaagtc
    16801 tattagaaat gttgttaaac ctctattaaa gtatgcacat catcgaggta tatatacaca
    16861 aatgaattca aacctaacat tgcctcaaga tcgttattta gatattgctg aatatatcga
    16921 tgttatgcat atctcacaca actggggaac aactgatgaa ttcgcaaatg ttggctttgg
    16981 cgcaatgaag aagcaaccac cgttaaaagc taagttaaaa ttatatgaac aaatgatttc
    17041 gaatgcacgt acattatcag aacaaggaat gtttgtatct gcggaaacaa tgctcaatca
    17101 aagtacgcta ccacatttac gaaaaataca tcaagaagtc gttcatgata tgaaatgtag
    17161 cagacacgag attcacccta tgtatccagc tgactttgca agtcaattaa atgtgttaac
    17221 tctagcggaa atgaaaaaga caattcatga tatattggat ttcagagatg aagatatttg
    17281 gatgttattt ggtactttgc ctgtgtttcc atgcttaaag gatgatgaag atcaaaagtt
    17341 actatcacgt ttaagaaatg ctaaaaatgt aacgactaga aatgacccgg atggccgtag
    17401 tcgtttaaat gtcaatgtat ttacaggtaa tgtaatcgta actgatttcg gagatgaaac
    17461 aggtacaatt tcgaatatac aaaaagataa attaacagat gtatttgata aatggttatc
    17521 ctctgatctt gctaaatcat taaattgtca ttgttccgag tttagttgtt taggaccaaa
    17581 tgttcttgtt aaaaatatgt actatccgaa tatggatttt aaagataatg agcgtcatat
    17641 gcacaaacaa ccacaaatta tacaatttta aaaactctta attatgcgga gaagcacttt
    17701 atcgataagt agtctccgca tattttaatg ctattataaa ataaaaaaca attaattgct
    17761 ggcagtactc tacttaaata ataaagggca tttaatagga ctaatagtct ataataaaag
    17821 gggtaaattt taactaaaag cataaacgtg cataatcaaa aagacagatt gtaggtggaa
    17881 tattcgaaca taacagttca attcatcctt aacaatctgt ctttatattt ttagtctcta
    17941 atatgttgca cttgagctaa atattcaatt gtttgtttac tttcaatgcg acgtgctttt
    18001 cttcgttcaa cacgttgtgg tgctgtatca taaaaccatt tttcaacatc atcttctgga
    18061 tatacaccag gtacatgttt aggtttgcct tcatcatcta acgcaacaaa tgttaagtaa
    18121 cttaatgcag ctaaatcatg cttgttatta aatacatcgt caattctaat ttgaacgacc
    18181 acttccattg aactagtccc agcgtatgaa accatcgcta cgtattgtaa tatgtcccct
    18241 gttttaatcg gctttaagaa atctactgag tctgtagatg cggtaactac ttgcgcacca
    18301 gcatgtttca tagctgtgat tgctgcaatt tcatcaatat tagccatcaa tgtaccacca
    18361 aacattgtat gatggtgatt cgtatcttga gggaaaactt gtctattttt ataacatttt
    18421 gattctgaca tagatttcat tggtctgtcc tgatttgtca ttattttatc tcccaatcta
    18481 tactttaatc acgtatttga cctttaaata catgtttcat ttcctactca gcacctcaaa
    18541 ataacattta ttaaaatgtt gatgcccaat ttccattttc aaatactagt tcttttgaac
    18601 catcttcgaa tatgccataa atagtcaaat cactactacc aatcatgaaa tcgacatgta
    18661 catttgaatc atttaatccg cttgcaatct tttcctcaac agtcatttcc gttccacctt
    18721 gaatattaaa ggcgtaagca gatccaatcg ctaaatgaca tgctgcattt tcatcaaata
    18781 aagtattgta aaaaatggta ttacgatttg atatcggtga atcatcaggt actaatgcta
    18841 cttcacctaa tcttcttgaa ccttcatcag tattgattaa atctttcaat accgcttcac
    18901 cttttccagc tgaaaaatca ataatctctc catctttaaa catcaattta aattgatcga
    18961 taattgtgcc attataactt aaaggcaatt tattagtaac atatccatct actctatttc
    19021 gatctggtgc tgtaaatact tcttcagttg gtatattagc aataaaagct tgttccttgc
    19081 cgttaacata acttgtagca tcttcccaga tatgattttt cgctaaacct actgttaaat
    19141 cagtaccttc agatacataa tgcaacgcat ggtaattttt ttgttgtaac ttttgagcgt
    19201 aaacacttaa attagcgatg tgttgacgcc aattttcaac tggatcgtta ccatcaattc
    19261 gcacaatatc gaatacctcg tcaataaact taatatatgc ttcttctaca gatagttcag
    19321 gatacactct cttcgcccaa gctttagatg gaaaagcagc tacgacccat ggaaattgat
    19381 ttttttgtac actttccata tatcctttaa atgcgcgtgc gttttgttgt tgaaatgctt
    19441 gtaatttttg gctatcgata ccatccatta aatcaggatc ttcactaata agtgctaaat
    19501 tagcagcgcc acgtttaacg taatccattc tcgcttcaac atcatatgat tttatctcat
    19561 gattagcaaa atgttccaca gattcatttt caaattttaa gcgttttaag gttggatcgc
    19621 tatatacaac acgtacatct gaagcgccac aatgatatgc ttcttctaca attaaatgcg
    19681 ttaattctaa tgtttctact gatgatctaa taaatactgg ctgttttggt tgaacattca
    19741 taccaacctt aactaatagt tcagcgtatt gttgtaactt ttctttgtaa ttagtcaaaa
    19801 ttcccactcc tattcgtgtc gcaattgtcc taaagcgtca gcaatagctg tcacttcact
    19861 tggcgacaat ctttctcttg attgaacaaa atcatatata tctgttaatt cttcttcatt
    19921 tgcattttta tatttttcag gatctaataa tccttgatta acaatattta acttctctct
    19981 aatagctaat actttctctt catttgtcat agccacaatg ttcacctcta ataatagtta
    20041 tgttatcaaa gtatcacaat ttaataaact ttaatagaat tttatataat ctgctttctt
    20101 attgtttaaa gtcgctataa aatggggata taactacata gtttggagga atcaaaatgg
    20161 tagatgtagc atttgtctgt cttggcaata tatgtcgttc tccaatggca gaagcaatca
    20221 tgcgacaaag acttaaagac agaaatattc atgatattaa agtacattca agaggtactg
    20281 gtagctggaa tttaggagag ccacctcatg aaggtacaca aaaaattctc aacaaacaca
    20341 atattccatt tgatggcatg attagtgaat tattcgaagc gacagatgat tttgattaca
    20401 ttgtggctat ggatcaaagt aacgttgata atattaaatc tatcaatcct aatcttaagg
    20461 gacaattgtt caaactgtta gaatttagta atatggaaga gagtgatgta ccagatccat
    20521 actacacgaa taattttgaa ggtgtatacg acatggtatt atcatcttgt gataatttaa
    20581 tagactacat cgtaaaagat gcaaatttga aagaggggta gttttttatg gaaaataaat
    20641 ttgttcctgg tattttaatt ggtgccgtaa ttggtggtgc aattagttta gctgataaat
    20701 ctacacgtca agctttagtt caatcagtta aagatgcaaa aaatggtaac cgcactcgta
    20761 agccttctaa agtcagcaag attaaagacg aagttttata ctggaaagat gttgttgaag
    20821 aaattcgtcg taataatcct gaattagaac gttcattaaa ggatgcgaaa gaaacatttg
    20881 ttaatagaaa aaatcaacgc taagatgatt ttgtttttat aaggcaatac ttaacaattt
    20941 ggtgtacgcc tttcatagtt atcattatta aatgataata caatgattgg ccaaactgca
    21001 ttgtttcagt attgcctttg ttttaatttt aaatcaaaat agcctatgaa agatttaaat
    21061 caattaattt ctataatatt atcattttta aagcatatca ttgtttagtt tttttataat
    21121 tggataaata ctaatagtta ctttataaaa cattacatag agaaaggtta aggagtgcac
    21181 atgtcgaaaa aggatcactc ttcttcaaaa taccttaatt ctgttaagga agcgcaagag
    21241 gagtcaaaaa agaaaaataa aagtaatccc aaaattgatg ttgatcgtac atatattgaa
    21301 cctcaacaat tccaatctaa gaaacctaaa aaagatgatc aggttttctt cttatcaaga
    21361 ttaaataaac ctgcaaaata taagaaagac tctaatttct tatcatatct catctatcgc
    21421 ataggaaaag atgatgcctc aggactagca gcacaaatga cttaccattt cgtacttgct
    21481 atgttcccta tgttgctttt cctattaaca ttattaccat ttttcaatat taagcagagt
    21541 caaattacta atatgttaag caatgcaccc gctgaaacat ctactctaat taagagtgta
    21601 attggtgata taactcaaaa ctccagtggt ggcttattat ctatcggttt gattttagca
    21661 atttggtcag cttcaaatgg aatgactgca attatgaatt ctttcaatgt tgcttacgat
    21721 gtagaagata gccgtaatgg aatcgtatta aaactactaa gtgttgtctt cactgtagtt
    21781 atgggcgttg tgtttgtagt tgctctagca ttaccaacgc ttggttctgt aattagtcat
    21841 ttcctattcg gtccacttgg atttgacgaa caagtgaaat ggatttttaa ccttattaga
    21901 attgtgttac caatcattat tatatttatc atatttatcg tgttatattc ggttgcacct
    21961 aacgttaaaa cgaagcttaa gtcagtatta ccaggtgcag tatttacttc aattatttgg
    22021 ttagctggtt catttggttt tggttggtat atttcaaatt ttggtaacta ttctaaaaca
    22081 tatggcagta tcgcgggtat catcattttg ttactatggt tatatatcac aagttttatt
    22141 ataattgtcg gtgctgaaat caatgcaatc attcatcagc gtagtgtaat taaaggtaaa
    22201 acacctgaag aagcagcatt agaacatgat gacaataata aaaatcatta taatgagaac
    22261 actagatacg aatatgatga agatagtaat gcaacacatc aacgtacgta tcatgttgat
    22321 gaacatccta gtgataccta tccagaagat gataaaaata taaaagacaa aatcgttgat
    22381 aagcttaaaa aagactaaac accaacaaaa cagaggtttt cgctaatgat tattgcgaaa
    22441 acctctgttt tttggtgtct atttgatgtt atattgtgat tggcgtaagt aacttttctt
    22501 aattcgatat gaactattga attaaattat gttggaatgc atagataaca gcttgtgttc
    22561 tatcttgcac ttctaactta cttaaaatgt tactcacatg tgtcttaacc gttttaatag
    22621 taatatgcga tgcactagca atttcttgat ttgagtaacc tttcgcaatc aataataata
    22681 tttccatttc tcgttctgta agcatttcat ataactctgc gcgctttttc atacggttac
    22741 gcattttcac taaaacttcc ggttcaaaaa cagattctcc tctagaagtt ttacgaactg
    22801 catcggcgat atcttttgca cttgttgttt ttaaaatgta actatcgaca cctgcatcta
    22861 atgcacgata tacctcttta tcttcaataa aactagttaa cattaatact ttaatttgcg
    22921 gtaaatcttt tttaatctga gtcgtcgctt ctacaccatc catgtcatcc ataagtaaat
    22981 ccattaaaat taaatctggc ttcaactcat gggctttggc aattgcttct ttaccagaag
    23041 cgccttcacc aactacttca atatcacttt gcgttgatag ataacttgaa attcctatac
    23101 gtaccatttc atgatcatcc acaaacaata ctttaatcgt catacgaatc ctccttattt
    23161 aaaggtgctt tcacctcgat acgtgtacct gaatctggca atgatacaat atggaacgtt
    23221 gcaccaattt ccaaagctct ttcacgcata tttttaagtc cataactttg ttctaatttt
    23281 tcatcaacat taaaaccttt accattatct tgaattctca acaataaata atcgtcttta
    23341 ttaaacaatt ctactgtcac ttttgtaccg tttgaatgac gcaatgtatt cgaaattgct
    23401 tcctgtgtaa ttctgaacaa atgatcttca atacctttag gcactttaaa atcttgtatt
    23461 tcatgcacaa ctttcattgg cacttttttt tgtaaatcaa taactaaatc tttaataccc
    23521 tcacctaaag atttgtcttt taaaccaagc ggtcttaaat gtaacagcaa agcacgcatt
    23581 tctaactgcg aatcttgaac cattttctct aaaataggaa tttgttggtc taatggtggt
    23641 tctaacttcg tttctttgat agcagatagc atcatacttg ccgcaaaaag ttgctgacta
    23701 acagaatcgt gaagttctcg tgctagtctt tgacgttcat cttcaataat ctttttaact
    23761 ttcacatcat taatattata attttcattg gttaagtttt gagttttaag tcgcaactta
    23821 tgcaattctt gatttaaagg tacgagtgta tggtataaat ctaacgtttc actatatatt
    23881 tctatatttt gatcattaat gccaactgtt tcgccttcca ttgaacgctc aatttgcgtc
    23941 ttaatccaat cattttgctg attgattttg taagcgagta ccgaaccaac aataatacac
    24001 aataatatga tgatgagatt taaaaataaa aaaactggta ttccgaatat ttgtgtataa
    24061 aacatacctt gaaaatagat gatatttaca aaaactttat cgatgaacag aaatgcagct
    24121 agcatgctat atactaagat gagcattgaa ccaattgttc taatgtagtg gttcatcgat
    24181 aaatcacctc tacgtctccg ataaacgttg atacgtagat attaactgta tagttatccg
    24241 gtttcatcat ttcttcaata tgaatattgt tattttcaac tttatatgat ttttcattca
    24301 cgtaagtact tccataaaaa gcagctacat gtaaattaat attgtaatta accggcaata
    24361 taacctgcac tttacctaaa atgtgtctaa caacaatggt attattttcc ttaatatttg
    24421 cagcttttgt taagtcaata tgtaggtcgc caattccatg ttgaatttgt acatcttccc
    24481 acttatatac ataaactggt gtacgttgct caccaaacca cttttgttta ataaaagatg
    24541 gtgaagtcat aacctcatcc gtcgcaacta ctttttttgg tttaaacttg tgtattaaat
    24601 aacgcacaat aagtaataat aaaaagacga acaagatgat gattgtatac ttattagaca
    24661 ataatgtgaa tgcaattaat agcgcgccta tccaaaacgc caaaaggcca cgtattttat
    24721 gaaaataaag atatcctaca taaactaata cacatcccaa taatagaacg agtaaaaagc
    24781 caattttttc aaaaaatatg tagtaaaaat tggcaataat cattaatgca gtaaaaatga
    24841 tcaacatttg cgttgatata tatttgtgtg tcatgtttcg ccgcctttct atgccacagc
    24901 gttcatataa tttattagat acgatgtttt ttctgttgct attttttcag ttgaagtttg
    24961 ttgaatttga gtttcaattt gtgcataata tgcttcaact ttattcaact ctgatttaat
    25021 attttcaatt tcttgattat tgatttctct tgcacattga atttgaaatg cttcagcaat
    25081 atcaatatat ttattttcta atgttagcat tagactgtca attaacttgc taagttgttc
    25141 ttttttagta attaaataac gcatataatc atctaaattt ttcatttttt catctagcat
    25201 taaaaggtag tgcttgatat tatttcttac tgttgctctc aaaaactgtt caatataacg
    25261 ttcaacatag ttcataacta tcacctttta taataagttt taaaatacca aatgcgctat
    25321 gtatcttcgt tgatgtcgat gatatgtttg cttaaaaact actttgttac aacatatttt
    25381 tgaaatgacg cattgattgt gttcttaatt attataaaac agttccgaaa ccaatatgaa
    25441 taggctccgg aactaaatga tgttcatact ttagtcttag tatatgttga actattcttc
    25501 ttcaatcttt gtcgttaaaa tcggaccatc cttagtcacg ataaccgtat gctcaatttg
    25561 agcaacaaaa cttttatcgc tcgtttcaaa agcccattca tttttacctt ctgtaacaaa
    25621 tgatgcattt gatgagataa acggttcaat agctaatacc ataccttcag ttaataatgt
    25681 tttgtctttt ggatcaaagt aattaagtac atgtgctggt gcttcatgta atgataaacc
    25741 aacaccatga cctgttaagt ttttaatgac tttcaaatca ttttgtctag ctgtattatg
    25801 caccgcttta ccaatgttac ttaacttagt acccggtttt acttttgcaa ttgcattctc
    25861 aaatgccatc gttgctacgt cacatacttt ttgtttcatt ggatcatctg attctccaac
    25921 gacaaatgaa atgcctgtat ctgcataata gccattcttc aaagccgata catcaatatt
    25981 tactaaatct ccttcacgaa tgacacgctt acttggaatc ccatgtgcca cctcttcatt
    26041 gacactaata cacgtttgac caggaaaatt ttcatcatga attggcgcag aaatagcacc
    26101 gtattcttca aataactctt tcgcaatatt atcaagctct ttcgtagtga tacctggttt
    26161 ggttgcagct tgcattgtat tgcgcacttt agcgcatatg tatccaattt cttttaacgc
    26221 ttgtaattct tcttctgttt ttacaatcat tttatcccgt tcctttttta tataattata
    26281 acttattata gcaaaaattc tttgatatac taaagatgtt actttagtat tttggaaagt
    26341 gagtgagaca tagtgtgata aagaaaaaat atttctttat cgttgtaccc caacttgcat
    26401 tgcctgtaga aattgtagat ccaatttctc tatgttgggg ccccgccaac ctgcacatta
    26461 tcgtaagctg acttttcgtc agcttctgtg ttggggcccc gccaacctgc attgcctgta
    26521 gaatttcttt tttgaaattc tctatgttgg ggccccggac tataattgaa aaatgcttgt
    26581 tacaagtgca ttttatttca gtcaactact aacaatataa cattgtggag cccagaactt
    26641 tgattaatgt atatgaaaat caaagtaatg cttatgtatg attatttcaa atattttaca
    26701 tacatgaact tttccaatgt acgatactat tattataaag cgctcgctaa gattttacga
    26761 tgattagagg ttaaaaaatg aacgatcaat ggtataaaca tttaattggt gccagaacaa
    26821 ttaaaactgg tattgccatt tttttaacag ctgtcttttg tatggcacta gatttaacac
    26881 ccatctatgc cattttaaca gctgtagtca caattgaacc aactgccaag gcatcactta
    26941 ttaaaggtta tcgtagatta cctgctacgg taattggtgc aggctttgca gtattattta
    27001 catatttatt cggcgatcaa tcacctttta catatgcatt gagtgcaacg ttcacgattt
    27061 tattctgtac aaaactcaaa ttgcaagttg gtacgaatgt cgctgtacta acatcattag
    27121 ctatgattcc aggtattcat gatgcctaca tattcaactt tttgtctcga acattgacag
    27181 caattattgg actcgtgaca tcaggtttaa ttaacttcat ggtgtttcca cctaagtatt
    27241 atggtcaggt cgaagaaaaa ttaagtaaga cagatgcctt gatgtataaa ttattttaca
    27301 atcgctgcca agaacttatc ttatcaagac tgcaatctga taaaagcgaa aaagcatata
    27361 aaaatatttt caatttaaat aatcaagttg aaacgttaat aagttatcaa cgtgatgaat
    27421 taagctatca taagaaaaaa gaatgtgatt ggaaattact caatcaatta actaaacgtg
    27481 catatacgaa tcgtttgttt atcactcatt tatcaaatat catttacttg cctaaaaata
    27541 ctcgtgttaa cttttcaggt gacgaaaaaa tggcattgct aaaaattagt agtagcatca
    27601 aagatatatt ttatgatggt tcttttaaga gagaagatga ttctgtagaa actttacgtt
    27661 cgactataaa agcacttgaa attagtggtg aaaatcaaat taaaagtcat atactatacg
    27721 aagttctaat gatttatcga ctgctagaca gcagatatgc ttaatttaag taccttatgc
    27781 taattggcac catgggagtg ggacagaaat gatattttca taaaatttat ttcgtcgtcc
    27841 caccccaact cgctttgcct gtagaatttc ttttcgaaat tctctgtgtt ggggcccaca
    27901 ccccaacttg cattgtctgt agaaattggg aatccaattt ctctttgttg gggcccctga
    27961 ctagaattga aaaaagcttg ttacaagcgc attttcgttc agtcaactac tgccaatata
    28021 acttcgtaga gcatagaaca ttga
//
LOCUS       DALAGF010000024        27954 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.024, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000024 DALAGF010000000
VERSION     DALAGF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 27954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27954
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.024"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            91..456
                     /locus_tag="O2692_000993"
     CDS             91..456
                     /locus_tag="O2692_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HCZ4298631.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            638..1915
                     /locus_tag="O2692_000994"
     CDS             638..1915
                     /locus_tag="O2692_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCZ4298632.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
     gene            complement(2319..2675)
                     /locus_tag="O2692_000995"
     CDS             complement(2319..2675)
                     /locus_tag="O2692_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCZ4298633.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     TTIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
     gene            complement(2940..3362)
                     /locus_tag="O2692_000996"
     CDS             complement(2940..3362)
                     /locus_tag="O2692_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HCZ4298634.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKIDL"
     gene            complement(3433..5538)
                     /gene="rsp"
                     /locus_tag="O2692_000997"
                     /pseudo
     CDS             complement(3433..5538)
                     /gene="rsp"
                     /locus_tag="O2692_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
     gene            complement(7026..7409)
                     /locus_tag="O2692_000998"
     CDS             complement(7026..7409)
                     /locus_tag="O2692_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCZ4298635.1"
                     /translation="MKERGYIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDVALLIEPSIGSVCNAFDHMWGYLKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
     gene            7592..9034
                     /locus_tag="O2692_000999"
     CDS             7592..9034
                     /locus_tag="O2692_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HCZ4298636.1"
                     /translation="MNYKQSAEEILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            complement(9120..10064)
                     /locus_tag="O2692_001000"
     CDS             complement(9120..10064)
                     /locus_tag="O2692_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCZ4298637.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(10110..10733)
                     /locus_tag="O2692_001001"
     CDS             complement(10110..10733)
                     /locus_tag="O2692_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCZ4298638.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            10894..12171
                     /locus_tag="O2692_001002"
     CDS             10894..12171
                     /locus_tag="O2692_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCZ4298639.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGVQRFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKS
                     VPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
     gene            12378..12809
                     /locus_tag="O2692_001003"
     CDS             12378..12809
                     /locus_tag="O2692_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HCZ4298640.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            12914..13030
                     /locus_tag="O2692_001004"
     CDS             12914..13030
                     /locus_tag="O2692_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298641.1"
                     /translation="MANYAIYFNNININSIMALNDNNYQFEHVNERNEYRES"
     gene            13041..14075
                     /locus_tag="O2692_001005"
     CDS             13041..14075
                     /locus_tag="O2692_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ4298642.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            14111..14512
                     /locus_tag="O2692_001006"
     CDS             14111..14512
                     /locus_tag="O2692_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4298643.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSLIMEHIMQYIKGVAVESTYV
                     SLIADYPADKLYTKFGFIPTEPDSGGMYIKY"
     gene            complement(14558..15562)
                     /locus_tag="O2692_001007"
     CDS             complement(14558..15562)
                     /locus_tag="O2692_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCZ4298644.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            15753..16268
                     /locus_tag="O2692_001008"
     CDS             15753..16268
                     /locus_tag="O2692_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4298645.1"
                     /translation="MAGIIKEISEQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            16582..17211
                     /locus_tag="O2692_001009"
     CDS             16582..17211
                     /locus_tag="O2692_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298646.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDGNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNQANN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKNQALQQLPNFQTALNAANNEANKFGSNNK
                     VYNDYSIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(17364..19037)
                     /locus_tag="O2692_001010"
     CDS             complement(17364..19037)
                     /locus_tag="O2692_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCZ4298647.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKSNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            19354..20952
                     /locus_tag="O2692_001011"
     CDS             19354..20952
                     /locus_tag="O2692_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCZ4298648.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTFTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            21333..22811
                     /gene="mqo"
                     /locus_tag="O2692_001012"
     CDS             21333..22811
                     /gene="mqo"
                     /locus_tag="O2692_001012"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCZ4298649.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSILFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVGIQYKQNVLDIKKQKSGV
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(22877..23332)
                     /locus_tag="O2692_001013"
     CDS             complement(22877..23332)
                     /locus_tag="O2692_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HCZ4298650.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVVYVLIWIIQFFKNKNYVDTINKQLKQLK"
     gene            complement(23329..23772)
                     /locus_tag="O2692_001014"
     CDS             complement(23329..23772)
                     /locus_tag="O2692_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4298651.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDNVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            complement(23935..25308)
                     /gene="hssS"
                     /locus_tag="O2692_001015"
     CDS             complement(23935..25308)
                     /gene="hssS"
                     /locus_tag="O2692_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HCZ4298652.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNQDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVNQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSL"
     gene            complement(25301..25975)
                     /gene="hssR"
                     /locus_tag="O2692_001016"
     CDS             complement(25301..25975)
                     /gene="hssR"
                     /locus_tag="O2692_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HCZ4298653.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            26111..27166
                     /locus_tag="O2692_001017"
     CDS             26111..27166
                     /locus_tag="O2692_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4298654.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            27166..27831
                     /locus_tag="O2692_001018"
     CDS             27166..27831
                     /locus_tag="O2692_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298655.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
BASE COUNT         9607 a         4009 c         4823 g         9515 t
ORIGIN      
        1 ataaaaaaat ttgttacaat ctggtaaaaa ctctttatta aataaattga ttcgttcata
       61 ctgaatagat aagcaaaaag gagttacata atgaagaaga aaaaaaacgc gattaaagtt
      121 atcattatat tatttatttc attaattgtt gcagtagcat ttttctttgg tctgaaaaca
      181 tatcaaggcc ataagaatat tcaactaatt gacagttact tggaagagaa aaatctaaaa
      241 gataaaatta aatcagaaaa aacagagtat agtgctaaaa aaggtctctt ctataaggaa
      301 gttacattta aagatgaacc tggtgtaact tatgttgtgc aaccaatcag tactaataaa
      361 ggattatttg ttgaaggctt tgatacagag actaagaaaa gtttgaaaac tgcaaagcat
      421 aaatatttca atcaaaatta taaaccatca aaatagtgaa acgttaaact ggtgaagcag
      481 tttagtgcct tcaattttaa ttatttgagg taaacagttt ttgtatggaa attagtgatt
      541 tttgacatct ataaaagata aatattttaa ttttttatag ataattatca attttctgat
      601 ataattgagt gatgtcagat gaaggggagt ttgtactatg gctctattca agagaaaaat
      661 tagcctacct atgcaagttg ttattgcatt agtgttaggt gttgtcgtag gacttttatt
      721 atatggacaa gaaaacgtag caaattacat taaaccattt ggtgacgtat ttttaaattt
      781 aattaaaatg atcgttatac cagttgtatt ttgctcacta gcgctttcta tttcgaacgt
      841 tggggaatcg aaaactgtag ggcgttatgg ctggaaaaca attttatact ttgaaattat
      901 tacaacaatc gcaataggtt tagggattat cttcggtaac ctatttaaac caggtgctgg
      961 attagaccca acaaaattac ctaaaggtga tatttctaaa tatcaatcaa ctgcacatgc
     1021 agcagaacaa tctacatatg gaaatcattt tattgatacc attgtacata ttattccgac
     1081 aaactttttt gaagctttaa ataagggtga attattacct attatcttct tcgcagtatt
     1141 ctttggatta ggattagctg ctgtaggtaa aaaagcagaa ccagttaaag aatttttaag
     1201 cggatcgctt gaagctgtgt tctggatgat taataaaatt ttaaaattag caccacttgg
     1261 agtgtttgca ttcatttgta ctacaattat tacatttggt gcatccgcat tattaccact
     1321 attaaaatta gtattagttg ttgtctttgc aatggtgttc tttgtattcg ctatactagg
     1381 actagttgca tggatgtgtg gtattaatat catgaatatt attagaatct tgaaaagtga
     1441 attgctttta gcattttcta catcaagttc ggaagctgta cttcctgtaa tgatgaagaa
     1501 aatggaaaac ttcggttctc caaaagaaat tacttctttt gttataccaa ttggttatac
     1561 gtttaactta gatggatcag cactttatca atctattgca gcattattcg ttgcacagat
     1621 gtatggaatg cacttaacat tatcagagca aattgtgttg atgttaacat taatgattac
     1681 gtctaaaggt atggcggcag taccaggaac ttcaatcgtt gttttattaa caacattagg
     1741 tgccatgggc ttaccggcac aaggtttagc attaattatt ggtgttgacc gtatcttaga
     1801 tatggtacgt acatgtgtaa acgttattgg taatgcatta tcaacaatcg ttatagctaa
     1861 atgggaaaac gtatatgaca aagcaaaagg tcaagaatat ttaaaatcaa tttaaaaaat
     1921 actatctgac atttaaagcc ccttacaacc tttggttgtt aggggctttt atgcatgcgc
     1981 taaagcgcag gcgaacggaa gcgtagtgac ctcaggttgt tagggacttt tatgcatgcg
     2041 ctaaagcgca ggcgaaccga agcgtagtga cctcaggttg ttaggggctt ttatgcatgc
     2101 gctaaagcgc aggcgaaccg gagcgcagtg acctcaggtt gttaggggct tttatgcatg
     2161 cgcaaagcgc aggcgaacgg caagtggagc tattcaatat gaaagtattt caaatataaa
     2221 aagcagcagt aaaatgattc caaattggaa atcaacttac cgctgctttt actcgtatat
     2281 accttgatgg caactaaaat ttgtaattta aattacaatt agtgtttcat gtgcattttg
     2341 cccatgtcat gaattttcat cattaaacca tgtggtacat cgtcatggtc aactttttta
     2401 actttaacaa ttttatctga atctgctaat ttataaacaa ttacaaaatc acctttttta
     2461 actttaacat ctgaatctga aggtaattta acattttttt caatactttc atctttttca
     2521 ctaatcattt tttcaatagt agtattatct ttcataattc catagtatgt atctttcttt
     2581 ccgccagtca tcatcatgat tactaataca atacatacaa ttaacatgat ggcgattaaa
     2641 ctaataccaa gaccaaaacc ttttttcttt ttcatggttc atcaatctcc ttaaatgtag
     2701 ttaattctgt tatacctaaa agtgcaacag tttgcaaatg cagtatttat attacataaa
     2761 attaatttaa aaagacacac cctaatcacg ttaaatgtga ataaagtgtg tctaaaaaac
     2821 aatacattgc aaattgaatt gtaaacatat taaacacttt aattatagcg agttatttta
     2881 agtatttaac attgttatat ctagaattca taaaagttat ttcctatata cactttgtgt
     2941 tataaatcga ttttgatagg tgtatctaat gatttatcat tcattagctt aatggattgc
     3001 ggaactactc ttaaaacaca taactctgga tcttcttttg aactgaaaaa gcttttgtct
     3061 tgtgtttccc atagccaatc aatcacttct tgttctgtaa cgatttcgat tgtcgcttcc
     3121 atctcaacga aactacgatt agttgtgtcg ttatagccta acaaaacata tgctgctgga
     3181 ttatctttaa tttctttgac cttagcagaa tggatattcg ttttagtata taaagtaaga
     3241 ccatcattat aaaagaccat atatctacta ttaggtttat tattatatgc agttgataat
     3301 acaccaactt ttgacgttgc taacacgttt tcaattgctt gaattgcttg tgagttactc
     3361 attacaaatc atctccttat gatataaata gcctttaacg taagaaacta aacacaagag
     3421 gactgttagt atttagcttg gtttaaagca aatatattta acctcgtcgt gatttaactt
     3481 gagttcaaat gtacgaccag tttcttcttg aactgcaagc tccgttttag ggtaattcgt
     3541 tttgtctaat tcatctaaaa tttctttttc tatataaaca agttgatcgc ttattggaag
     3601 caaatgttga attgaaccat gttcatgatt caatgtttta ataatcatca aagagtcttg
     3661 aggtaattca ttatggaaaa tgaaatcttg ttttacgtct gacatatatc gatcattaat
     3721 tttattgaat aataagaaat ggtagttatt atctttacga ctcaatacat aattatttgt
     3781 aatttcaata tcttcattaa caaaaggtct aaacatttta tataaatgca ttaatggtag
     3841 ggggctacca tatttgttgt acaaggaaat ataatttgaa tggtcatcta ataattgata
     3901 gctaatgcca ccgccatatc gtaataatgc gattaagtga tacactatgt cagataattg
     3961 aattggattc ttaccatcat tagtatattt aaatccgctt acagacaggt tattatatac
     4021 aaattgtgtt gatgatatac ctgaatctaa aataaatttc ttatactgtt caaaatgagt
     4081 gtcagtttga tgatagtgtt ggtttttagt gataagatgt gttgcagttt ttgaattttc
     4141 aatatcaata aagtaataat ggaatttaag acgctgcatg acatcataaa cttgttccac
     4201 ggttgaataa gtttctaaaa agccatcgac tgtcacacta tatttaattt ctttattttt
     4261 gtttttaatt ttaagatgac ataaatgaat ttcttctgct gtcatagaat tagagcaaaa
     4321 tacgagcata aatttaacta atttcttgct tgtcttatag tcttgattgc cttgtaaaaa
     4381 tgttagtatt aattgcttca tcgtttcgaa ttgttgtgtt gatttaatag cgattgctaa
     4441 acctatattt ttttcgaaaa gcttttcaaa acatctattt aacaggttgt aattcgtttg
     4501 gctaatctca atatcgtgaa tatcgtcaat gaaaacgaca ggttttggta agtgctcaaa
     4561 gttaatatcg taatattcat tgaaaataaa ttgaaatagt tcattgaaat tactaaatcg
     4621 aacaaatact ttggcacgct gacttcgatc tttcggatta aaatcatcta tatgaatatc
     4681 tgttgtattt gtagccagtt gagctgttaa atcaattgtt gaaattaact cagtgaattg
     4741 tgatacgtta tcttgttgga agtgtatttg tggtggttca gtaatcttgg aaacgagtga
     4801 acggaactgt ttaggactaa aatgcaagta attcttaaat tgatttgcaa aatttgtatg
     4861 actgttaaaa ccggccaatt cagaaacagt tgtaatagaa tgttttgtag aaagtaataa
     4921 attgatggcg ttaacaagtt taatacttgt aaaataatct ttaaaattca tactcaaata
     4981 tcgaacaaaa agattagaac aatatgattc agagatgttg caatgcatcg ctatatcttt
     5041 taaagacaag tgcgcatcaa tattgtcatg aatatagttt acacagtcta tgaacaccgg
     5101 attgcttaaa gcaatatttg gtaaatattc atgatctatt cttataaaag cttctttaag
     5161 taaagtgtct ataatacttt gacctatatt tttccccatt aaagaagaac ttgttggtgt
     5221 actgattaaa tcggcaatta atgacttaat ataattactt gattgtaata aatgacgatc
     5281 taaataacca ttaaagaaat catcatcttg ttgataaaaa taaaaaacgg gtatcattaa
     5341 ttcgattata ttttcgccgt ggttaatatt ataaatatca ccatggttaa ttaagataat
     5401 ataattactt acgtctttgg tttaaccatt aatcgtaata ttgagtttac ttgtaagtga
     5461 aaatagcacc ataatattat taatattacg acttggtatt gtcgataatg tatgatgtat
     5521 tttaagttgg catgtcatat ttctctctcc tgcctaatat atattgaaat ctatcattta
     5581 aatgtaaaac atggaaattt ttaaaatcat ggatagtata cctattatag ttgaatattt
     5641 ttgaaaaggt aatagtgaaa aataaaagaa attaatgaaa actcctagga aaacgcttgt
     5701 ttttatgcat tgcgaacaat ataaaagtaa aattagaaag atagtaagca aaatttggaa
     5761 attttcgata gagtctaatt aaattatttg gtgcataatt aatatagatt tcaaacctat
     5821 gtatttcaaa aaacaataca tagccatttt gaactaactt gctaatcttt tattgatatt
     5881 caactatgtt ataaaaaaag caaattgacc aaaatggaag tttgaatcaa aaaacattaa
     5941 atgtacccat gacttaaatt ttatcgccat tatttaagtt tggacataca cttttgtcat
     6001 caaacgctga aaattagtac gattgaaatc acttcttgtt ataaaaattc ttagtagccc
     6061 tgttacaaaa tattcgctac aaattattca aatcaacaac aataaccatc aactaagaaa
     6121 ttttaaacaa tctactaaaa aataaagcgt ttaaaagttc tgtttagaaa acatcaagat
     6181 aagtataaac atctagaaaa gtgaacatct aagaaaatcc atctagttat tgctgtaaca
     6241 attagaataa tcatttgaaa aagtgcatta gacaacgaaa ttcaccatca agataatgaa
     6301 gcaacaagaa acagcacaca tcaatgaatt aacaccttag attcatcatg agaaaatgca
     6361 tggcacaact agatttacca tcaagataat tcttaagcag cgaagaatta acatctagaa
     6421 atgcataaac atcttagatt ttacatcaag agatgcgata actataaaca actagaaacg
     6481 tcaccaagat agtgcaacaa caaagaatca gcatcttgaa atcgcactaa catctaagaa
     6541 tcaacatcaa gtattatatt aacatcatag ataacttcat caagacaggc atccacatct
     6601 aagaaactca tctagaatag ctaacaccta acatcaaaga atattcatca agaacagtat
     6661 ctaacagaac tttttgacta tatatgaatt aattatgtta ctagctaaat catctattat
     6721 taactttcac actcaatttc tacatctaat atcaatttat taacaataga attagcagca
     6781 acaagatatt acttaaaatt actcccccga atttaaaata taaacaatgt aataattaag
     6841 ggtataaatt gatttatgct gtaactaatt aaatgtgtat ggtatcttct aacatcatga
     6901 aacgcatttt aatatagata tgtacattta aaccttgaga gatagattat agtaaacttt
     6961 agtacaaagt atgaattgct gaactccaat gactactatt tgtccgttac aactaaagtt
     7021 tcaatctatc tctttctttt tgtgtttaaa acacgacttt gtagtaaata ttgaacatca
     7081 tatttattag caagttctgc taaaaaatct aataaagttt gggtgtcgat tttaccatta
     7141 ataaaatcag ctttaagtag ttttgattgt tgtctttctt cttcattcgc acattttttt
     7201 aaatatcccc acatatgatc aaaagcatta catacacttc ctatactcgg ttcaattaac
     7261 aaagcaacat caatcatttg ttgaacttga tgaagtgata aatccgtttt taaagcattc
     7321 cttatcattt ggtaagactg ttgactgtgt aacaatacat gatatttttc ctcacgccaa
     7381 agctgttcga tatagccacg ttctttcata aaattacctc cgataattaa tgttaatata
     7441 catgcatgta gtatagcaat aaaaatgaat gcgctatcaa aaatggtgta ggtgtttata
     7501 taaaatgata atagagtgtt gcaaatattt ttggaaccgg ttacaataaa gtggaataaa
     7561 tggaaccggt tctaattagg aggctaaata tatgaattat aagcaatccg cagaagaaat
     7621 tttgaacgcg ataggcggag aagagaattt agatgcaatg gcgcattgtg caacgagact
     7681 acgattagtt ttaaatgatg aaagtttagt aaatgaagag gcgctaaaca atatggatgt
     7741 agttaaaggg acgttttcta ctgggggaca ataccaaatt attattgggt ctggtacagt
     7801 caataaagta tttagtgaac tggaaaaatt aactggaaaa gaagcatcaa ccacttcgga
     7861 agtcaaagca caatctgcta aaaatatgaa tccgttacag cgatttgtaa aaatgctttc
     7921 agatatcttt gttccgatta taccagccat cgttgctggt ggtttattaa tggggttaaa
     7981 taacattttg actgcgaaag atttattctt ttcaggtaaa tcattgatag atgtatatag
     8041 tcaatttgct ggattagctg aaatgataaa tgtttttgcg aatgcaccat ttacattatt
     8101 accaatttta attggattta gtgcagcaaa acgctttggt ggcaatccat ttttaggtgc
     8161 tgcattaggt atgatactag ttcatccatc gctaatgagc gcatacgatt tcccaaaagc
     8221 agttgaagca ggtaaggcta ttccatattg ggatgttttt ggtttgcata ttaatcaagt
     8281 aggttatcaa ggacaagtgt tacctatgct tgtagcagct tatatcttag cctcaattga
     8341 aaaagggtta cgcaaagtta ttccaacggt gttagataat ttgttaacac cattgttatc
     8401 tatttttata acagcatttc taacattttc atttgtaggt ccaatcactc gacaattagg
     8461 ttactggtta tcagatggtt taacatggct ttatgaattt ggtggtgcaa ttggtggatt
     8521 aatattcgga ttattgtatg ctccgattgt tattacaggt atgcatcata gctttatagc
     8581 tgtagaaacg acattaattg cagatgccac taaaacgggt ggatcattta tattcccgat
     8641 tgcgacaatg tctaatgttg cacaaggtgg tgcagcaatt gcagcgttct ttattattaa
     8701 acaaaataag aagttaaaag gtgtggcatc tgccgcaggt atttcagcat tacttggtat
     8761 tacagaaccg gctatgtttg gtgttaactt aaaactaaga tatccattta ttggcgctat
     8821 cgttggatca ggtattggtt cagcatatat tgctttcttc aaggttaaag caatcgcatt
     8881 aggaactgct ggattgccag gatttatttc aatcaatcca gtacatgcag gatggttaca
     8941 ctactttgtt ggtatgacaa tatcattcat cattgctata acagttactt taattttatc
     9001 taaaagaaaa gcaaataaag aagttgtaga ataaaaaaag aggtatgaca gtaatcaaag
     9061 tatcagtcat tgcaaatgca ataggatgaa actttaagct gtcatacctt ttaatttagt
     9121 tataatttct gtttacgcca taagaaaata ctaacaatta cgacaagaat taatgaaata
     9181 cctaccacaa taatccaaga tatataacta tggtcatcaa ttggcaacgg cacattcata
     9241 ccgaagaaac taaatacgag tgttggtaac gttaatagta ccgtgaaaag tgttaatgtc
     9301 ttcataatcg tattcatatc attagataat aaggaagcgt atgatgttgt gatactttct
     9361 aagatacgtt gatgtaattc tgttgtttcg atggcttggt tattttcaat gattaaatct
     9421 tctagtaatt cttcatcttc ttcaaaacgt ttaattgcag gtaaacggaa taacttttta
     9481 ataattgtat cgttaccttt taaggcagct aaaaagtata ctaaactttt ttcaacttcc
     9541 attaatttga aaagttgttt gttagtaatg ttatttttta gttctttctc aatacgaatt
     9601 ctacttttat taagtaatcg taagttacga ttataatggt cggcaatagt agttaaaatt
     9661 tctaatgcaa atctgctgtg aaattttaaa ttaatatcac gcttaggtaa attttctaaa
     9721 aattcatttt cagcgtcaca aactgttacg ataataccgt tgccaataat aatgcctaat
     9781 ggaatcgtta caaaagatag aacgctacga ttagttgaat tgacgatagg taaatcaata
     9841 atgattaatg agtaaccagt atcttcgtca tattcaatac gggaactttc ttctgagtcc
     9901 aaagggtcac gtataaagtc ttcaggtata ttatattgtt ccataagatt ttcaatttct
     9961 tctcggtctg gttctactac attaatccat gacgctgtat gatctaatgg tgtttttata
    10021 atatgtttat ctatatcttc agaatgtctg aatgaagtaa tcatgatata tagttccttt
    10081 ctaaaggatt taggaaaagc gagtattact taagttagtg aatcaaggtt tgatttatgc
    10141 aaacggtttt ctgccattat tcttaatgga ccattaaaaa taatattatg cacatgttta
    10201 gctaattcat caactgaaat gggctgtttg tccattaccc agtatttaat aattgaaatt
    10261 gttgctccgg aaacgtaact gtaaaaatac atctctggaa cgcctccgat agaatgattg
    10321 atactaatgt aacgttgcat attattttta atatgttcgt tgattttcaa ttctaattga
    10381 ctcgtgcgtt ccaattgtaa aatagtatga taaaactcta aattatcatg aatatgttgg
    10441 aaaatatttt tgagtatatt atttacaaaa gtattcgcaa tgtcttcaat tgaagaaccg
    10501 cgtaacaaat caaattgagt atatgtcgtt agttctgaaa tatactcatc ttccatatct
    10561 gcgagtaaca tatatttatc ctcgtaatgt aagtaaaatg tccctctatt gatatcagcg
    10621 cgtgttgtta tgtcgcgaat ggtaatcttt tctaaatctt tttcttgtag taatttagtg
    10681 aacgcttgtt taatggagga ctttgtcttt ctaattcgcc tatcttcttt caaaaataaa
    10741 tcaacctcct tgtgttattg aacaatgtgg gtgtatttgt ctattaatga acgtattgat
    10801 gaacattgtg tattgtttat tcccacttac atattataat agacaacatg cgaaatattc
    10861 aacacattgt ctaataagga ggtaattaat aaaatgaata tatttaaaaa taaattatta
    10921 tggattgcac caatagcaac tatgattatc ttggtaatct tttctttagc tttttatcct
    10981 gcatataatc ctaaaccaaa agatttacca attggtatat taaacgagga taaaggtaca
    11041 acgattcaag ataaaaatgt taacattggt aaaaaattag aggataaatt attagatagt
    11101 gattctaata aaattaaatg ggttaaggtt gatagtgaaa aagaccttga aaaagatttg
    11161 aaagatcaaa aaatctttgg agtagctatt attgataaag acttttcaaa agatgctatg
    11221 agtaaaacac aaaaagtagt tatggatagt aaaaaagaag aaatgcaaca aaaagttgct
    11281 tcaggtgaaa ttccgccaca agtggttcaa caaatgaaac aaaaaatggg gaatcaacaa
    11341 gtagaggtta agcaggctaa atttaaaacg attgtaagtg aaggatcaag cttacaaggt
    11401 tcacaaattg catcagctgt gttaactggt atgggtgata atattaatgc tcaaattacg
    11461 aagcaaagtt tggaaacatt aacgagtcaa aatgttaaag tcaatgccgc ggacatcaat
    11521 ggtttgacga atccagtaaa agtggataat gaaaaactta ataaagttaa agatcaccaa
    11581 gcaggtggta atgcaccatt cctaatgttt atgccaattt ggataggttc aatcgtaacg
    11641 tctatcttat tgttctttgc atttagaact agtaacaata tcgtcgtgca acatcgtatc
    11701 attgcttcaa ttggacagat gatatttgca gttgttgcag catttgcagg tagctttgtt
    11761 tatatttatt tcatgcaagg cgttcaaaga tttgattttg accatccaaa tcgtatcgca
    11821 atttttgtag catttgcgat tcttggtttc gtgggcctta ttttaggtgt tatggtatgg
    11881 ctaggtatga agtcagttcc aattttcttc attttaatgt tctttagtat gcaacttgta
    11941 acgttaccta aacaaatgtt gcctgaaagt tatcaaaaat atgtatatga ttggaatcca
    12001 ttcacacact atgcaacaag tgtaagagaa ctattatact tgaatcatca tattgaatta
    12061 aatagtacaa tgtggatgtt tatagggttt atgatttttg gcgctgtatc aagtttagtt
    12121 tcagctattg ttagaaaaca tagcacaaaa cgtactgaag tgccgtcata atttaataaa
    12181 tagaaataat agctatacaa gacgtttgta gcgagcgtca tggtatagct attttatttg
    12241 tatagcgctt gatgtaaagt tattttagct gtcatactat gtgctaaacc aaattcagtt
    12301 gtgtcatatc ttcgcttttt tgtcactgtt ataataggta taatcggata taatgaaaag
    12361 gtgagggagg attcaaaatg cgtaggttat tatattcatt tcttttttat atgatcatag
    12421 gtttatttag tggctttttc tatagagagc taacaaaagc atatgatttt actggtacaa
    12481 cacaattatc acttgtacac acacatacac ttattttagg tatgtttatg tttttaatat
    12541 tattaccatt ggagaagtta tttaaattat caagttacta cttatttaac tggttcttct
    12601 atgtatataa cataggtgtt atcgttacta taggtatgat ggtgacaaaa ggattcttcc
    12661 aagtcactgg taaatcatac tctccagaag catttgcagg ctttgcaggt attgggcaca
    12721 caggtatgct tgcaggttta ttgttgctat ttttcctttt aagacaagca attcttaaag
    12781 aaccacgtga tgagtctttt aaaaaataat ataactagaa ctaaaaaata tacacatctt
    12841 tcaaaggaac attgttgttt cttactttga aaggtgtgtt tttatattgt gatgggatag
    12901 ttattgataa ttcatggcta actatgcgat ttattttaac aatataaata ttaatagtat
    12961 aatggcttta aatgataata attatcaatt tgagcacgtt aatgaaagaa atgaatatag
    13021 agagtcatag gaggcacata atgaaagatg ttacaatcat tggtggcggt ccgtctggtt
    13081 tgtatgcaag cttttatgct ggattgcgag atatgtctgt aagattgata gatgttcaat
    13141 ctgaattagg gggtaagatg agaatttacc ctgaaaaaat tatttgggat attggcggca
    13201 ttgcgccaaa accttgtcat gaaattttaa aagatacaat taagcaagga ttatatttta
    13261 aaccggaagt tcatttgaat gaacgtgtgg tagatattag gaaaaaggca gaacgtcatt
    13321 tcgaagttga aacagaagcg ggagaaatat atacatcgaa agcagttatc attgcaattg
    13381 gtgcgggtat tattaatcca aaacaattag atgttaaagg tgtagagaga tatcaattaa
    13441 ctaatttaca ttatgttgta caaagttaca gacgtttcaa agataaagat gtattaatat
    13501 caggtggagg aaatacagca ttagattggg cgcatgatat tgccaagatt gctaaaagcg
    13561 tgacagttgt ttatcgaaaa gaagatgtaa gtggtcatga agcgatgaaa acgttggtga
    13621 cagatttaaa tgtgaaacta tgcccaaaaa cacgtattaa atacttggtc ggcaatgatg
    13681 atgaaacgca tataagtgaa gttgttttgg aacatgttga aagtggcgat aggcatacag
    13741 ttaaatttga cgatgtcatt attagtcatg ggtttgatcg ttgtaataca ttattgagtg
    13801 aaacatcttc aaagctagat atgcatgatg attgtcgtgt taaaggattt ggtaatacga
    13861 caactagtat acctggtatc tatgcgtgtg gagatattgt ttatcatgat gcaaaatcac
    13921 atctaattgc aagtgcattt agtgatggtg caaatgcagc gaaccttgct aaaacatata
    13981 tccaaccaga tgcgaatgca gagggttatg tttcaagtca tcatgaagtg tttaaagaag
    14041 cgaataagac tattgtaaat aaacatttat actaggttga tatattgtgt gatgaaaagt
    14101 ggggtaaata atggttaaag tgacatatga tattccgact tgcgaggatt attgcgcatt
    14161 aaggattaac gcaggtatga gtccaaagac gcgcgaagca gctgaaaaag gattacctaa
    14221 tgccttattt acagtaacct tgtatgataa agatcggtta attggtatgg gtagagtgat
    14281 tggcgatggc ggaactgttt ttcaaattgt tgatattgca gttttgaaaa gttaccaagg
    14341 tcaaggttac ggcagtctaa ttatggagca tattatgcaa tatattaaag gtgtggctgt
    14401 tgagagtaca tacgttagtc tgattgcaga ctacccagcg gataaattat atacaaaatt
    14461 tggatttata cctaccgaac cagattcagg cggtatgtat atcaaatact aaattaaaaa
    14521 agacatctcg cataacggga tgtctttttc atgaatacta tttatctaca ccgaaatcga
    14581 taacaatgcg ccctttattt tcatgtttaa ttactttgtt aagttgttct ggaagttcat
    14641 caaatgtaat aacttgcttg atctcatgta aattttcagg cattaaatct tttgcgagac
    14701 gacgccaaac gcgctgtctt aatttcatag cagtaaatac cgagtcaatt cctaaaatgt
    14761 ttacacctct taaaatgtga gggaatacag aattagtata agtattaccg gcagtcatac
    14821 caataactgc aattgaccca ctatgattta aacgctttgt aacataatta ataccttcgc
    14881 caccaacagg gtctacacaa gcttgccaag ttgatgatgc gagtggcttt ttatgatcat
    14941 cttcaacagg aagtcgatcg ataacttctt tggcaccaag ttcaagtaat tgatcgctaa
    15001 catcttgttt acctgtactt gcgataactt tataaccaag ttcattaagc atgagtactg
    15061 ctaaagtacc gacaccacct gaagcaccgc gaacgagtac aggaccatct tcaatattca
    15121 ttccaacttt ttcaagtctt tcaattgcta aaccggcagt ataaccagct gtgccatata
    15181 tcattgattc ttctaatgtt aaagtatcag gaagcttgat aatccattct gattttacac
    15241 gcgcatattc actaaaaccg ccataatggc tgacacctag gtcataactc gttacaatta
    15301 cttgttctcc tttttcaaag cctggtgctt cggattcaac aattgttcca gctaaatcta
    15361 ttcctggaat cataggatac gattttacga ctgcattatg atcttgagtc gctaaagcat
    15421 ctttataatt tataccagag taatgtactt taatcagcac atctccttta ggtaaatcag
    15481 taggcgataa ttgtttgaaa gttggtgtca ctttaccact ctcatcttta tcaattacaa
    15541 acgctttaaa tgtttctatc acaacgatac atcctctctt tttctctttt gaaaatacgg
    15601 ccattttact ggaataaatg tatcatttat tgatgttaca cacaatcaat acaatttgta
    15661 attgatttgt gttaatgtat ttttaaatag ttttggctga aaaataacat aagtaatatt
    15721 ttgattcgat cactgtttat tggaggattt atatggctgg gataatcaaa gaaatttcag
    15781 aacaaaatgc tagtgaatta gttgaattag caactagaac attttatgac acgtttggtt
    15841 cttactatga tgacaaagat tttgatcaat tttttaaaga caattatact gtagagaaat
    15901 ttacacaaga gattaaccat gtagattcat ttcattattt ttatcaagaa gatggtgcga
    15961 atgttggtta tataaagatg aatattaata gtgctcaaac tgaagaaatg ggggagacct
    16021 atttagaagt gcagcgcata tattttttga aagactttca aggtggcgga agaggttcac
    16081 aattgataga attggccgaa aaaattgctc aagaacataa taaacataaa atttggctag
    16141 gagtttggga gcataatcct cgtgctcaag cattttataa acgtcatgga tttaaagtgg
    16201 ttggtgaaca tcattttcaa acaggtgatg taacggatac agatttaatt atggaaaaag
    16261 agttataatg attgtttttg cagcagataa aatgaaatca tagtaataaa tattgtgtat
    16321 accttgaaat gctaaatgcg taatgaattg ttaaagtaaa ataattaaat ttatagaaaa
    16381 cataaaattc acaaaaatta aataataatg aacaaaaatt agggtataat aaaaagaacc
    16441 ttagattgta gttatggata ttgtgataat tcagatcata ttaatgttga cgtctttggt
    16501 aacaagccat gaaaagatac acatggtaga tatgtatttc aagattattc aatgaatatc
    16561 gaattatagg aggagatatg tatgaaaaga ttagttacag ggttactagc attatcatta
    16621 tttttagctg catgtggtca agatagtgac caacaaaaag acggtaataa agaaaaagat
    16681 gataaagcga aaactgaaca acaagataaa aaaacaaatg attcatctaa agataagaaa
    16741 gataataaag atgatagtaa agacgtaaac aaagataata aagataatag tgcaaacgat
    16801 aaccagcaac aatctaattc aaatgcaaca aacaatgacc aaaaccaaac aaataataac
    16861 caatcaagta ataaccaagc gaataataat caaaaatcaa gttacgttgc accatattat
    16921 ggacaaaatg ccgcgccggt tgcacgtcaa atttatccgt ttaatggaaa taaaaatcaa
    16981 gctttacagc aattgccaaa tttccaaaca gctttaaatg cggctaataa tgaagcaaat
    17041 aaatttggta gtaataataa agtgtataat gattattcta ttgaagaaca taatggcaac
    17101 tataagtatg tgtttagttt taaagaccca aatgcaaatg gaaaatattc aattgtaacg
    17161 gttgattata ctggacaagc aatggttact gatccaaact accaacaata atgttaatat
    17221 aactatgatg caagttaaaa aataaaacgg taaactctat aatatgaatt agggtttacc
    17281 gttttttgcg tattttaaag tatcaaagtt tacgttttat agtactttta aacctcattc
    17341 acagccactg agaaatttaa acactattga ttagatgtca tcttagacaa tataagttgt
    17401 gaaatagatt ctaatggttg ttctttatgt tgtatttgtg gatatttaat cactggtatt
    17461 gcctgggaaa ttaaattatt aattaataat tgtcggtctg tataccaaat ttttgataga
    17521 tgacgccaaa tatcttcgtt aacaccttga gactcaatgt attgactgtg atttggagtg
    17581 tataatgcta ctgttttgtc tatagccatc gcatcaaata ttatatttga gtaatcagtg
    17641 ataacgacat cagacattag aattaagtct tgagcactaa gatactttgg tgcaacaagc
    17701 gcctcttctg gtaagatcgc ttcatcaaca ccctgaacca caacatgata ggctttgaat
    17761 aaagcatctg atagtggtaa ttgttgcgca ctcactaatc caataggtgc gtataataaa
    17821 actggttttt gatcatttat tttaaacgat ttcttatact gttgttgagt agtactttcg
    17881 ttactctttt gtagtaaata ttggtgttta ggattaccat aagccaatac atttgcttgg
    17941 tgactaggaa aagccgtttg ataaaagtgg ctaatatcat cagcagaatg tataacataa
    18001 tcgaattgca accagcgatt atacctttta gcacgataaa aaggtgtttc tatattttta
    18061 ataggctctt tactatctaa aaataattgc attattggtg tgccttgatg taattgaatt
    18121 aaagtaccgt ttggttgtaa cgtttcaggt atatcatttt caactataac tacgcgtgcg
    18181 ctgttaatca attcgtcagc tttttcagac ttaggtgata tgaaatgtgg tccgcgacga
    18241 tcgtcagtaa caaaataaca ggcagtcatt ggattatgtt taacgaagta agtaaataaa
    18301 tattttgaat tacctatata ttgatgatga tcaccgataa aaacgatgtg atcttttaaa
    18361 acgtttggtt gcttcgtata caggccgaag ttttgtaatt gtttgttatc agcacgttgc
    18421 tttgctattt tatataacca tttaggtagc ataaacttgg tagaatacaa ccaattatct
    18481 ggcgaattaa gttgttctga atcctcgata aaaaggttgc gaaaaggtat aggatgatga
    18541 tcaatataag ccattttatt caaataaaac tgagacatca tatcaaaatc ttcaatagtt
    18601 ttaatagttt cccatattga agcacttgtt gctatagggg ctttaaaatc aaaaacgaca
    18661 tgaccatcga ttgataaatc acctttcaga agtgtcaata aagctaaatc gaaaatcgtt
    18721 tctacagagt aataatgtat taaatcaaga gtctgttgaa tcgcatatat caggttagga
    18781 taataattat tgttttgagc ccaatcggca atgttgatcg atgattgacc gaaatagtga
    18841 caatcatttt tgtattgtcc atccacagta taatcaacca cgatagactt aggatataat
    18901 tgaaccgcat ctaacatcga ttgataaatc attatctcat tggcatatgg aacgacatgt
    18961 gtataacctt cagactgagc tttcattaat tgagattcaa acttttgcat attagaaaca
    19021 tttatctgtt taatcattgc tataacttcc attcctcatc gtagtatctt ccttatttta
    19081 gcacggtctt atgtaaaatt catgaatagt ataagttact tcattacatt aagtacaatt
    19141 cattttgatg aacagattta aaatgaaaaa taaaacaatt aaataggaaa gtgatattgt
    19201 taatagtctg aaaaaatggt atattttaat tgtgaaaaaa atcacattaa tctcactgtt
    19261 ctgtaaaatg ttatcgataa caaaatgcta tagccagagc atgattttaa tgaagcaatt
    19321 acactactac tttatggaaa gaagagtcag cctatgttag taaatacgtt taacccattt
    19381 gataatttat tattatcgtc tttaattgca gcaataccga ttgtactgtt tttactatgt
    19441 ttaactgttt ttaaaatgaa aggtatttat gcagctatta caacacttgt tgtaacatta
    19501 ctaattgcaa taccattttt caaattgcca gttggtatcg cttctggtgc agtagtcgaa
    19561 ggtttcttcc aaggtatcat tccgattggc tatatcgtta tgatggcagt attgttatac
    19621 aaaattactg ttgaatctgg acaattttta acaattcaag atagtattac aaatatttca
    19681 caagaccaac gtattcaagt tttacttatt ggatttgcat tcaacgcatt tttagaaggt
    19741 gcagcaggat ttggtgtacc aattgcaatt tgtgcacttt tattaacaca attaggattt
    19801 aatccattaa aagctgcgat gttatgttta gtcgcaaatg cagcgtctgg tgcttttggt
    19861 gcgattggta tccctgtagg tgttgtagaa acgttgaaat tacctggaga tgtttcagta
    19921 ttaggtgttt ctcaatcagc aactttaaca ttggcaatca taaatttcat tattccattc
    19981 ttacttatct ttattattga tggttttaga ggtgttaaag aaacattacc agcaatttta
    20041 gtagtttcaa tcacttatac atttactcaa ggattattaa ctgtattcag tggacctgaa
    20101 ttagcagata ttattccacc gttattaaca atgttagcat tagcagtatt ttctaaaaaa
    20161 ttccaaccaa aacacattta tcgtgttaat aaagatgaag aaattgaacc tgcaaaagca
    20221 cattctgcaa aagcagtatt acatgcatgg agcccattca ttgtattaac agtcattgta
    20281 atgatttgga gtgcgccatt ctttaaaaac ttattcttac caaatggtgc tttatcatca
    20341 ttagtattta aattcaactt acctggaaca atcagcgaag ttacgcataa accattagta
    20401 ttgactttaa atattattgg acaaacaggt acagctattt tattaactat tattattaca
    20461 attttaatgt ctaaaaaggt taactttaaa gatgcaggta gattattcgg cgttacattt
    20521 aaagagttgt ggttaccagt tcttacaatt tgtttcatct tagcaatttc taaaatcaca
    20581 acttatggtg gtttaagtgc agcaatgggt caaggtattg ctaaagcagg taatgtcttc
    20641 ccagttctat caccaatttt aggttggata ggtgtgttta tgacaggatc agttgtaaat
    20701 aacaactcat tatttgcacc aattcaagct tctgttgcac aacaaattgg aacaagtggt
    20761 tcacttcttg tatctgctaa tacagttggt ggtgtagcgg caaaattgat ttcaccacaa
    20821 tcaattgcaa ttgcaactgc agcagtaaaa caagttggta aggaatcaga attattaaaa
    20881 atgacattga aatacagtgt atgtttacta atattcatct gtatttggac tttcatcttg
    20941 tcattattat aaaaaaacgt atttcaaaat ataaatatac agaaggtgag atgttttcta
    21001 acatctcatc ttttttttat ggatcattaa tgaaagaagt ttgacattat aataatggta
    21061 gcgctttatg ttaaaatgaa tagtgagtaa tcagcaatca aattaaattg gttgatagct
    21121 gttaaggttt gtggttttgt ctttgtgcta tcgcacataa agtatataat taaagtagtt
    21181 tcgttattat aaaatattaa tatacatagt agatagtaat agagcatcac catgggaacc
    21241 tattgagaca cttattgatt taaagtggta ttaatatgtc gtatttctcg aacgttccat
    21301 tattcatttt aaaaaggggg actgtatttg ttatgacaac acaacatagc aaaacagatg
    21361 tcatcttaat tggtggcggt attatgagtg caacattagg aacattactt aaagaattat
    21421 cacctgagaa aaatattaaa gtgtttgaaa aattagcaca acctggcgaa gagagttcaa
    21481 atgtatggaa taatgccggt acagggcatt cagcactttg cgagttgaac tatacaaaag
    21541 aaggtaagga tggcacagtt gattgtagta aagcaattaa gataaatgag cagtaccaaa
    21601 tttcaaaaca gttttgggca tatttagtta aaacaggaca attagataac ccagatcgct
    21661 ttattcaagc ggtgccacac atgagttttg tcattggcga agataatgta gcttttataa
    21721 aaagtcgtgt tgcaacgtta aagaaaagta ttttattcga aaaaatgaaa ttatcgcaag
    21781 atgaagaaga aatgaaatct tgggtaccgt taatgattga aggtcgtaag tctgatgaac
    21841 caattgcttt aacttatgat gaaactggta cagatgttaa ctttggtgcg ttaactgcaa
    21901 agttatttga taatttagag caacgtggtg tgggaattca atataagcag aatgtattag
    21961 acatcaagaa acagaaatct ggggtatggc tagttaaagt taaagattta gaaactaatg
    22021 aaacgacaac atatgaatct gattttgtat ttattggtgc tggcggtgcg agtttaccat
    22081 tactccaaaa gactgggatt aaacaatcaa aacatattgg tggtttcccg gtaagtggat
    22141 tattcctgcg ctgtacaaat caagaagtga ttgatcgtca tcatgctaaa gtgtacggaa
    22201 aagcagcagt gggtgcgcca ccaatgtcag tgccgcactt agatacacgt tttgtagacg
    22261 gcaagcgttc attgttattt ggtccatttg caggtttctc acctaaattt ttaaaaacag
    22321 gttcacatat ggatttaatt aaatcggtta aaccaaataa tatcgtgacg atgttatctg
    22381 caggtatcaa agaaatgagt cttacgaagt atttagtgtc acaattgatg ttatctaatg
    22441 atgagcgtat ggatgattta agagtctttt tcccaaatgc taaaaatgaa gattgggaag
    22501 tgattacagc agggcaacgt gtccaagtaa tcaaggatac tgaggattct aaaggtaact
    22561 tacaatttgg tactgaagtt attacgtcag atgatggcac attagctgca ttacttggtg
    22621 catcacctgg tgcgtcaaca gctgtagata ttatgtttga tgttttacag agatgctatc
    22681 gtgatgaatt caaaggatgg gaaccaaaga ttaaagaaat ggtgccgtca tttggttatc
    22741 gcttaacaga tcatgaggat ttatatcata aaattaatga agaagtaact aagtatttac
    22801 aagttaaata ataaacgaaa cggtaatgtc ttttttaatg tgatagacat taccgttttt
    22861 tagtggttaa taaaaatcat tttaattgtt tcagttgctt gttaatagtg tctacgtagt
    22921 tcttgttttt aaagaattga attatccaaa ttaatacata aaccacaatg aagataattg
    22981 tgaatatgat tagataatgc actgttagtg gaaaccaacc ggcaagcatt gctaaaggca
    23041 agaatccgac atacgttgtt atgaaatgca ttatagttgc tttagtaatg ctccaatctg
    23101 tgtatttaaa gataaaatct ccaaggaaaa agacgacgcc tatgagtaac cataaaatga
    23161 tagaaatcaa cattacggta gtttctgtga aatgcgtata atacaatatg ccaatagttg
    23221 attgtgggtt cagtggataa tatttgccgt ctgcaaataa catactaaag aacagtgaaa
    23281 gggacaaacc aatgattaag ctaataaata atgagttttt caaatttttc atattgataa
    23341 gcgctccttt atagatttta aataacgtct agaagaatag gtgtagtgtg catctttaag
    23401 atacatacgt ataagtccat ttggctctaa taataatttt tcaatgtaat acttgttgac
    23461 gatttctgat ttggaaatgc gaatgaaatg ttgtggtaac tgtttttcta gttcataaag
    23521 tcgtaatttt agtttgaatt tttgattgct tgtaatggct gttacatttt tattaataac
    23581 ttggaatgtt ataacatctg atacattaat gatatggatt tctttgtcta tgtatccgac
    23641 taatgtatgt gatttgtcta aatcattgac tgcattaata atactttgaa cgttatcatt
    23701 cattttaggt gcatgtatat caatataaga ttccgtctca tttgcattga taaataaatt
    23761 gagtttcatc ataggttaat gcctccttca aaattattaa accataaatg accatcgata
    23821 tatttaaatt ttgttgaatg gtagaaatta aatgttaagt ggctagaaag cgctaatcaa
    23881 tataaaagat acctcctgaa ataaaaacag aaatgttttt tcaggaggta gagattaaag
    23941 tgaattattt ggcagtgtaa tagtaaaggt ggttacatac tcgttacttt gtgtgaattg
    24001 gattgtacca tgatgcaatt caatgatgga ttttgtaatt gcaagaccta aaccattgct
    24061 attatcatgt ttgctcactt tataaaaacg ttcaaataaa cgtgcttcag cttgtggact
    24121 aattggtgaa ccatcattac ttattgtgaa aatgatattg ttgtgactat gttgcaaagc
    24181 gatgtcaatg gcaccaccaa catctgtata cttaatagca tttattaata aattactcaa
    24241 tgcttgatgt aacaaacgtt gatttcctag gaaattgatt gattctaggt cagctaaaat
    24301 gattaacgac ttttcatcag cagcaaattg ttcatgtcga atgatatctt taatgagttg
    24361 attgacttga atcttatcat cgaataataa atgttggtga ttatctaatt cggataagag
    24421 caacaattca gttgttaatc cgctcagctg cgttgtaatg gtatagatat catttatata
    24481 ttgttgtctt aatgttttat cagaagtttg ttgtaactcg cttaataaat gatgaatatg
    24541 tgttaatggt gttttgattt catgagatac attttgtaca aaatgctgcc tcatttgatc
    24601 aacttgtccc aatgactctc tcatcttatt aaagtgatac tgtaatgttc caatttcatc
    24661 tttgcgtgtt tgtttgatag gtgtttcaaa atcaccatca attaagcgtt cggtcgctag
    24721 ctttaacttt tttactggac gaatgattga ataggttgat gcgataacta atgaaatcga
    24781 gataaataat aacaacatta acaagacggc taagaatgtt ctaaactcgc taaatgtttc
    24841 tccaatatct ggacgcataa atactgctat tgaaccgtct tttgtcttaa agttaatacc
    24901 aacagtatta tctgttacat tgtcgaaaaa tccagttacg aataatgcga atggtttatc
    24961 tttaatacca tggtaatctt gattatttaa tacgttgtta atagcatttt gtgataaagt
    25021 atcttctcta aatggctccc cataaaatgt cttatgacct ttttgatcaa tggtcattat
    25081 ttggtaattc atttgcccta aatgtttgaa atattgttga atgtgcgttg gtttagctga
    25141 ttgttcatat tgtcttgctt ctttaagcgt cttcatgatt ttcgcgtcat tagatgcttt
    25201 taaattataa tgatagtaaa catttgttaa tacaaaactt attaatgcac taaataaaat
    25261 aaccgtaatg gaataaatcg caattctagc atagagtgtt ttaaacatga ttctccacct
    25321 tatagccttg tcctcttact gtttcaattg taagtgtggc atttaatttt tttaatcttt
    25381 ggcgtagtcg cttaatatga acgtcaactg ttcgctcatc tccttcataa tcatagcccc
    25441 aaattttttc tattatttgt tcacgagtaa agatttgctt aggacgtgct gcaagcataa
    25501 ataataattg aaattccttg ttcggtaacg tcatcgtttt attagatact tggagttcca
    25561 aataggattg gtttagcgtt aagttgccaa tagtcatttc tgaatttgaa ttgatattat
    25621 atcgacgtaa tacagcacga attctaaaaa taagttcctt aacctcaaag ggtttggtta
    25681 cataatcgtc agtaccgctt ataaacgcac gctctttgtc actaagtgca tcccgcgctg
    25741 ttaacataat aactggtata tcataatcat tttttaatgt attacataat tgaaagccgt
    25801 ccataccatc catcataata tctaccactg caatatcgac acgctgtttt tctaaaagtt
    25861 ttaatgcagc ttcaccactt ggttgtgtgt atgcatcaat gtgctctgtt tgtaaatggc
    25921 tagctatata atttaggatt cgaggatcgt catcgacaac aagacattgc accatagcta
    25981 taaactccct tatctttttc atttattata catgtaaaat atttttgcgt aaaaaaacaa
    26041 ttgttcatat tgagttcata tttcaacctt atactgacgc taaagaagaa atagggagaa
    26101 gtgaatcgat atgaaattag cgataaaaga gattatgttt tacaaatttc gttatatttt
    26161 aatcacatta atcattcttt tattaagtat tatggtgtta tttattagtg gtttagctca
    26221 agggcttggt agggagaata tttcgttatt tgaacacttt gataatgatg aatatgttgt
    26281 tcaaaaaatg aaagagccgc aaattgagaa atcgcaactt agtgatacgc aacagaatca
    26341 aattaaaaag gtaattcatc aagaaccata taaaatgaat attcaaacat tgaaattatc
    26401 taataaagaa caagatgtga ttacaatgaa tgacgttaaa caacaacgca tacaacttaa
    26461 aaaaggtgat tatcctaaaa atgctcatga ggtcgctatt aatgataagt tagctgcaga
    26521 caacattaga gtcggggata gattacattt taaaaataat tcaactagtt atagagtttc
    26581 tggtatttta aacgacacaa tgtatgcgca tagttccatt gtgctattga acgataacgg
    26641 atttaatgca ttgaataagg ttaatacggc attttatcca gtgaaaaatt taacacaaca
    26701 acaacgtgat gagcttaata aaataaatga cgttcaagtt gtgagtgaaa aagatttaac
    26761 aggtaatatt gcgagttatc aagcagagca agcaccgtta aatatgatga ttgttagttt
    26821 gtttgctatt acagcaatcg ttctaagtgc atttttctat gttatgacga ttcaaaaaat
    26881 atcacaaatt ggcattttga aagcaattgg tattaagaca agacatttat tgagtgcgtt
    26941 agttttacaa attttaacac taacaataat tggggtaggt attgctgtga tcatcatagt
    27001 aggactatca tttatgatgc cggtaacgat gcctttttac ttaacaacgc aaaatatttt
    27061 attaatggtg gggatattta tattagtagc gattttaggt gcctcactat catttatcaa
    27121 attatttaaa gtggatccta tcgaagcaat tggaggtgca gaataatggc attagtcgtt
    27181 gaagatatcg tcaaaaattt cggagaaggt ttgtctgaaa caaaagtttt aaaaggtatt
    27241 aattttgaag tggaacaagg ggaatttgtc attttaaatg gtgcctctgg ttctgggaaa
    27301 acaacattgc taacgatatt aggcggattg ttaagtcaaa cgagtggtac agtgctttac
    27361 aatgatgcgc cattgtttga taaacagcat cgtcctagtg atttacgatt ggaagatatt
    27421 ggttttattt ttcaatcttc acatttagtt ccttatttaa aagtgataga gcaattgaca
    27481 ctcgtaggtc aagaagcggg aatgaccaaa caacaaagtt caacaagagc aatacaactt
    27541 ttgaaaaata ttggtttaga agatcgcttg aatgtatatc cgcatcagtt atctggcggt
    27601 gaaaagcaac gtgttgcgat tatgagagca tttatgaata atccgaaaat cattttagca
    27661 gatgagccca cagcaagttt agatgccgat agagcaacaa aagttgttga gatgatacgt
    27721 caacaaatta aagaacaaca aatgattggt attatgatta cacacgatcg aagattattt
    27781 gaatatgcag atcgagtgat tgaattagaa gatggcaaaa taactgatta gtggcttgta
    27841 aagacgctaa atgttaatga tttaagacat agtagtataa aagttagata acagaatacg
    27901 atttgggttt acaaaaaaca ggctgggaca ttaagttctt aggcaatgta aaaa
//
LOCUS       DALAGF010000025        27256 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.025, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000025 DALAGF010000000
VERSION     DALAGF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 27256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27256
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.025"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            64..1410
                     /locus_tag="O2692_001019"
     CDS             64..1410
                     /locus_tag="O2692_001019"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCZ4298656.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
     gene            1432..2247
                     /gene="ccsA"
                     /locus_tag="O2692_001020"
     CDS             1432..2247
                     /gene="ccsA"
                     /locus_tag="O2692_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HCZ4298657.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            2289..3215
                     /gene="hemC"
                     /locus_tag="O2692_001021"
     CDS             2289..3215
                     /gene="hemC"
                     /locus_tag="O2692_001021"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCZ4298658.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQNEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            3237..3905
                     /locus_tag="O2692_001022"
     CDS             3237..3905
                     /locus_tag="O2692_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCZ4298659.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            3908..4882
                     /gene="hemB"
                     /locus_tag="O2692_001023"
     CDS             3908..4882
                     /gene="hemB"
                     /locus_tag="O2692_001023"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCZ4298660.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            4930..6216
                     /gene="hemL"
                     /locus_tag="O2692_001024"
     CDS             4930..6216
                     /gene="hemL"
                     /locus_tag="O2692_001024"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCZ4298661.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGNDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            6277..6378
                     /locus_tag="O2692_001025"
     CDS             6277..6378
                     /locus_tag="O2692_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298662.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            6412..7485
                     /locus_tag="O2692_001026"
     CDS             6412..7485
                     /locus_tag="O2692_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCZ4298663.1"
                     /translation="MAMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKNNWLSIIVVSISILLIAIVMA
                     FVFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(7708..8268)
                     /locus_tag="O2692_001027"
     CDS             complement(7708..8268)
                     /locus_tag="O2692_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCZ4298664.1"
                     /translation="MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLQDCPSKPKHN"
     misc_binding    8326..8627
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8671..11301
                     /locus_tag="O2692_001028"
     CDS             8671..11301
                     /locus_tag="O2692_001028"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCZ4298665.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     gene            11314..12585
                     /locus_tag="O2692_001029"
     CDS             11314..12585
                     /locus_tag="O2692_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCZ4298666.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHV
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            12855..13562
                     /locus_tag="O2692_001030"
     CDS             12855..13562
                     /locus_tag="O2692_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCZ4298667.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            13559..14245
                     /gene="radC"
                     /locus_tag="O2692_001031"
     CDS             13559..14245
                     /gene="radC"
                     /locus_tag="O2692_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCZ4298668.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            14313..14597
                     /locus_tag="O2692_001032"
     CDS             14313..14597
                     /locus_tag="O2692_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298669.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(14714..14911)
                     /locus_tag="O2692_001033"
     CDS             complement(14714..14911)
                     /locus_tag="O2692_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298670.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            15071..15544
                     /locus_tag="O2692_001034"
     CDS             15071..15544
                     /locus_tag="O2692_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HCZ4298671.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKSENAYQ"
     gene            15618..15737
                     /locus_tag="O2692_001035"
     CDS             15618..15737
                     /locus_tag="O2692_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298672.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            15937..16779
                     /gene="mreC"
                     /locus_tag="O2692_001036"
     CDS             15937..16779
                     /gene="mreC"
                     /locus_tag="O2692_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCZ4298673.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            16779..17309
                     /gene="mreD"
                     /locus_tag="O2692_001037"
     CDS             16779..17309
                     /gene="mreD"
                     /locus_tag="O2692_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCZ4298674.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    17336..17463
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            17476..17784
                     /gene="rplU"
                     /locus_tag="O2692_001038"
     CDS             17476..17784
                     /gene="rplU"
                     /locus_tag="O2692_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCZ4298675.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            17790..18110
                     /locus_tag="O2692_001039"
     CDS             17790..18110
                     /locus_tag="O2692_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCZ4298676.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            18122..18406
                     /gene="rpmA"
                     /locus_tag="O2692_001040"
     CDS             18122..18406
                     /gene="rpmA"
                     /locus_tag="O2692_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCZ4298677.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            18761..20053
                     /gene="obgE"
                     /locus_tag="O2692_001041"
     CDS             18761..20053
                     /gene="obgE"
                     /locus_tag="O2692_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCZ4298678.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            20066..20521
                     /locus_tag="O2692_001042"
     CDS             20066..20521
                     /locus_tag="O2692_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCZ4298679.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            20535..21137
                     /gene="ruvA"
                     /locus_tag="O2692_001043"
     CDS             20535..21137
                     /gene="ruvA"
                     /locus_tag="O2692_001043"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCZ4298680.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            21169..22173
                     /gene="ruvB"
                     /locus_tag="O2692_001044"
     CDS             21169..22173
                     /gene="ruvB"
                     /locus_tag="O2692_001044"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCZ4298681.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            22175..23200
                     /gene="queA"
                     /locus_tag="O2692_001045"
     CDS             22175..23200
                     /gene="queA"
                     /locus_tag="O2692_001045"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCZ4298682.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSSRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            23223..24362
                     /gene="tgt"
                     /locus_tag="O2692_001046"
     CDS             23223..24362
                     /gene="tgt"
                     /locus_tag="O2692_001046"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCZ4298683.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            24381..24641
                     /gene="yajC"
                     /locus_tag="O2692_001047"
     CDS             24381..24641
                     /gene="yajC"
                     /locus_tag="O2692_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCZ4298684.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            24916..27195
                     /gene="secDF"
                     /locus_tag="O2692_001048"
     CDS             24916..27195
                     /gene="secDF"
                     /locus_tag="O2692_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HCZ4298685.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
BASE COUNT         9538 a         3729 c         5214 g         8775 t
ORIGIN      
        1 cttaattgga ttttaaatta tgttataata tttgtattgt tagtatatat gggggcgttt
       61 caaatgcatt ttattgcaat tagtataaat catcgcacag ctgatgttgc actaagagag
      121 caagttgctt ttagagatga tgccttacga attgcccatg aagatttata tgaaactaaa
      181 tctattttag aaaatgtcat attatcaaca tgtaatcgaa ctgaagtata tgctgttgtt
      241 gatcaaattc acacaggtcg ttactatatt caacgatttc tagctcgtgc atttggattt
      301 gaagtagatg atattaaagc aatgtcagaa gtaaaagtgg gggacgaagc agtagaacat
      361 ttattgcgtg tcacttctgg tttagattca atcgtacttg gagaaactca aattttaggt
      421 caaataagag atgcattttt cttagcgcaa agcacaggta cgacaggaac aatttttaat
      481 catctattta aacaggcaat tacttttgca aaaagagcac ataatgaaac agatatagct
      541 gataatgctg taagtgtgtc ttatgctgcg gtcgagttgg cgaaaaaagt atttggcaaa
      601 ttgaaaagta agcaagctat cattattggt gcaggggaaa tgagtgaatt atcactatta
      661 aatcttcttg gttctggaat tactgatatt acagtagtaa atagaacaat tgaaaatgct
      721 atgaaattag cagcaaagca tcaagtgaaa tatgatgaac tatcatcatt accaaattta
      781 cttgaaagtg cagatattgt gattagttca acgagtgcac aatcttatat cattacaaat
      841 gaaatgatag aaagaattgc agaaaataga aagcaagatt cactagtatt gattgatatt
      901 gcagttcctc gagatattga acctggtatt agtgccatca caaacatctt taattatgat
      961 gttgatgact taaaaggttt agttgatgca aacttacgtg agcgacaatt agcggctgca
     1021 acaatttcgg aacaaattcc tgcagaaata catgcacaca atgagtggat tagtatgttg
     1081 ggtgttgttc cagtgattag agctttacgt gaaaaagcta tggcaattca agcagaaacg
     1141 atggacagta ttgatcgtaa attgccaggg ttaagcgaaa gagaacgtaa aattatatct
     1201 aaacatacaa aaagtatcat caatcaaatg ttgaaagatc ctattaaaca ggccaaagaa
     1261 ttaagtagtg ataagaaaag taatgaaaaa ttagagctat ttcaaaatat atttgatatc
     1321 gaagctgaat gtcctcatga gcaagcgaag caacaaaaag aaagcaaggt caaggaaatt
     1381 tcagcgcgac gtatctttag ttttgaataa gcatattaat atggtgataa tatgcaagaa
     1441 aacctgttta ttcgattcaa tgaaattata ttattaatat acttaatcag tatcatttgc
     1501 tatttttatg attttgtaca aaaaagtcat aagattagaa gtttaggcat atatttattg
     1561 gggattgttt gggttttaca aacaatctct ttatctattt ttattataca aactagacat
     1621 attccattag ggtctatttc agatgtattc tatactttaa gttggctgat tatttcaatc
     1681 tctttaattc ttaaccttat caaagttttg aatttctctg tgtttttctt gaatttaata
     1741 gggttaacac ttttagggat gaatacattt caaccgacac attatactaa taaggttcag
     1801 aaaattgctg ttgtagacga gttactgtta gtccatatcg gtctagctgt gttaagttat
     1861 gtcttctttg ccttagcttt tgtcaatgca ttactgtata tcatgcaata ccgtaatttg
     1921 aaggaaaaac gttttgatca aaaatacttt agaattggta gcgttgctac acttgaatca
     1981 attgtgtttt actcaacgtt aagtgggtgg ataattctca tatttagtat tattttaggt
     2041 acacagtggg gtgtaatttc agttggtgaa cgtatattca ttgacccgaa ggtaatactc
     2101 tcttcaatta ttacagtatt atatggaagt tatattttac ttagaatcaa caaatggttg
     2161 aattcaagat atttaatata ctataacatt attttatttt gcttaaatat gattaattta
     2221 ttttttgcaa cacattttgt gaattgagaa aataatctag agcaatccgt tcctaggagg
     2281 aattgaacat gcgtaaatta gtcgttggct ccagaagaag taaattagct ttaacacaaa
     2341 gccagcaatt tattgataaa ttaaaagctg tcgagccaaa tctagaaatt gaaattaaag
     2401 aaattgtcac gaaaggcgat cgtatagtag ataaacaatt gtctaaagtc ggaggcaaag
     2461 gcttatttgt taaagaaata caacatgaac tttttgaaaa aaatatcgat atggcaatac
     2521 actcgcttaa agacgtacca agtgtaattc cggaaggttt aacattaggt tgtatccctg
     2581 atagagaatt accttttgat gcgtatattt ctaaaacaca tacaccacta tcccaattgc
     2641 cagaaggcag tattattggt actagttcat tacgtcgtgg tgcacaaata ttatctaagt
     2701 atcctaattt agagattaaa tggattagag gtaatataga tacacgatta gaaaagttac
     2761 aaactgaaga ttatgatgcg attattttag ctgcagctgg tttaagaaga atgggctggt
     2821 cagatgatat tgtaacatct tatcttgata gagatacatt gttacctgca atcggacaag
     2881 gtgctttagg gatagaatgt cgtagtgacg atgaagaact attaacatta ttaagcaaag
     2941 tacataatga tgaggttgca aaatgtgtga ctgctgaacg aacgttttta gcagaaatgg
     3001 atggtagttg tcaggtgcca attgcaggat atgctacaat ctcagatcaa aacgaaatcg
     3061 aatttacagg tttaattatg accccagatg gtaaagaacg atttgaatat acaatgaacg
     3121 gaacagatcc ggttgagtta ggcaaaacag tgagtaacaa attaaaagag caaggtgctt
     3181 atgaaattat aaaacgctta aatgaacaac attaaataat ggagtgacgt ttaaatatga
     3241 agccagttgt agttatgaca caaacaaatg acatgcaaag tgatttagta tcaattattc
     3301 acaaaccctt tattgatata aagccactaa attttgatat tcatttgtta aatcaacgct
     3361 atgactggct tattttttca tctaaaaatg ctgtgaaatt cttttataaa tatctaaaag
     3421 gaattaatgt tgataacatt gctgtgatag gaagtaagac agcgcaatat tgtgaatcac
     3481 ttggcattcg agttgatttt atgccaaacg acttttctca agaaggattt ttaaaatcat
     3541 ttaatcaaac taaccaaaaa atacttttgc cttcgagtga attggcgaga ccattgttat
     3601 tagcagcgtt atctaaagat aatgaagttg ttaaaataga tttatatact tcagtgccta
     3661 acaaacaaaa tatacaagat gttaaagaaa tgatagaaca tcaacaaatc gatgcattaa
     3721 cattttcaag ttcgtcggca gtacgttatt attttaatga aggatttgta ccaaaattca
     3781 agtcgtattt tgctattgga gaacaaacag cacggaccat taaatcatat caacaaccag
     3841 taacaattgc agaaattcaa acactcgaat cactaattga aaagatttta gaaagtaggg
     3901 gctaaaaatg aaatttgata gacatagaag attgagatca tcagcgacaa tgagagatat
     3961 ggttagagag aatcatgtaa gaaaagaaga tttaatatat ccaatttttg tagttgaaaa
     4021 agacgatgtg aaaaaagaaa ttaagtcatt gccaggtgta taccaaatca gtttgaattt
     4081 acttgaaagt gaattaaaag aagcttatga cttaggcata cgtgccatta tgtttttcgg
     4141 tgttccaaac tcaaaagatg atataggtac tggtgcatac attcacgatg gtgttattca
     4201 acaggcaaca cgtattgcta aaaaaatgta tgatgactta ttaattgttg cagacacttg
     4261 tttatgtgaa tatactgatc atggtcattg tggcgtgatt gatgaccata cacatgacgt
     4321 tgacaatgat aaatcattgc cactacttgt taaaacagca atttctcaag tggaagctgg
     4381 tgctgatatt attgcgccaa gtaatatgat ggatggtttt gttgctgaaa ttcgtcgtgg
     4441 attagatgaa gccggctatt acaatattcc tataatgagt tatggtgtca agtatgcatc
     4501 aagtttcttt ggacctttta gagatgcagc agattcagcg ccatcatttg gggatagaaa
     4561 aacgtatcag atggaccctg ctaaccgttt ggaagcactt cgtgaattag aaagtgatct
     4621 taaagaaggg tgcgacatga tgattgttaa acctgctcta agttatttag atatagttcg
     4681 agatgttaaa aatcatacga atgttccagt tgttgcatat aatgtgagtg gagaatatag
     4741 tatgactaaa gcagcggcac aaaatggttg gatagatgaa gaacgtgtcg ttatggaaca
     4801 aatggtttca atgaaacgtg caggtgctga tatgattatt acgtattttg caaaggacat
     4861 ttgtcgctat ttagataaat aaggttttat atttatgatt ttccataaac tgtaggagga
     4921 atttacttta tgagatatac gaaatcagaa gaagcaatga aggttgctga aactttaatg
     4981 cctggtggtg taaatagtcc agtacgcgca tttaaatcag tagatacacc agcaattttt
     5041 atggatcacg gtaaaggttc aaaaatttat gatatcgatg gtaacgagta tatcgactat
     5101 gtactaagtt ggggaccact tattttagga catagagacc ctcaagttat tagtcattta
     5161 catgaagcaa ttgataaagg tacaagtttt ggtgcatcaa cattacttga aaataaattg
     5221 gcgcagctcg ttattgaccg agtaccttca atagaaaaag tgcgtatggt gtcatctggt
     5281 acagaagcta cattggatac tttaagatta gcacgtggtt atactggcag aaataaaatt
     5341 gtgaaatttg aaggttgcta tcatggtcat agtgattcgt tattaatcaa agctggttct
     5401 ggggtggcaa cattaggatt gccggattct cctggtgtgc ctgaaggtat tgctaaaaat
     5461 acaattacag ttccatacaa tgatttagat gcacttaaaa tcgctttcga aaaatttgga
     5521 aacgatattg ctggtgtaat cgtagaacct gttgctggta atatgggtgt cgtaccgccg
     5581 attgaaggtt ttttacaggg attaagagat attacgactg aatacggcgc attgctaatt
     5641 ttcgatgaag taatgactgg tttcagagtc ggttatcatt gtgcacaagg ttactttggt
     5701 gtgacaccag atttaacttg cttaggaaaa gttatcggtg gaggactacc tgtaggtgca
     5761 tttggtggta aaaaagaaat catggatcat atagcaccat taggaaatat ttatcaagcg
     5821 ggtacgttat caggaaatcc tcttgcaatg acaagtggtt atgaaacgtt aagccaatta
     5881 acgccagaga catatgagta ttttaatatg ttaggcgata tacttgaaga cggtttaaaa
     5941 cgtgtatttg ctaaacacaa tgtaccaata actgtaaata gagcaggttc aatgattggt
     6001 tatttcttaa atgaaggacc tgtaactaat tttgaacaag cgaataaaag tgatttgaaa
     6061 ttatttgcag aaatgtatcg agaaatggca aaagaaggtg tgtttttacc accatctcaa
     6121 tttgaaggta cattcttatc tacggcacac acgaaagaag atattgaaaa aacgattcaa
     6181 gcatttgata cggctttaag tcgtattgta aaataaatat acggacaaat tgagagcctg
     6241 aactttgttc aggctctttt taaatgtata taaggcatgg gcggcgactt gatagtgaaa
     6301 gtccatatat gctttgtagt caaaactgct agcggatatt gttatcttaa caaacgtgaa
     6361 gctcaagcag caatttagtc attttatttt ttattgaaag aagtggaaaa catggcaatg
     6421 atatatagaa ataatttcat tgtgttcgtt ttatcatttt ttattagtat tctactgtat
     6481 tcatcgcacg tattacttcc atttatgttt ggtcctatta tcgcatcaat catttgtgta
     6541 aaagttttca aacttgatat taaatggcca ttcttactta gtgaattagg tattgtacta
     6601 ttaggtgtgc aaatcggatc aacgtttacg aaaaatgtcg ttatggatat taaaaacaat
     6661 tggctttcga ttattgttgt atctatttcg atattattaa ttgcaatagt aatggcattt
     6721 gtttttaaaa aaattgcacg tattaataca gaaacagcta ttttaagtgt tataccagga
     6781 gcactaacac aaatgctggt catggctgaa caagacaaac gtgctaattt gttagttgtt
     6841 agcttaacgc aaacatcacg aattatattt gttgttgttt tagtaccgtt catttcatat
     6901 ttttttcatg atggtaacat gcatgcgaat ggaaagttaa caaaagtctt gcctttatca
     6961 caagtattaa acatagggca aatagttatt ttagcgatag ctatctttat agtttatcta
     7021 attatgtcta aaataaagtt tccaacattt caattattag caccactcat tgtattaatt
     7081 gtttggaatt tttctacagg tttaacattt acactagatc attggttgtt gaacatggca
     7141 caactaatat atatgattag aattggagtt caaattgcgc atttattgtc agatttaaaa
     7201 ggtagactag caatcgcaat tacaattcaa aatattatgt tgataattgg tgcgctaatc
     7261 atggtttata tcatacattt ctttgataat aatcctatta atgaattgtt tcttggtgca
     7321 gcaccaggag gggttaatca gatagtttta gttgcattag ctactggtgc cgatgttgct
     7381 atgattacga gttatcatat tttccgaatc tttttcatcc tatttattat tgcaccagga
     7441 attaattact ttttaaaata tcgttcgaat aagcgtgaag attagaaact atcatccatc
     7501 taagttgaat tatgcttagg atagaattca ttattaaggt ttaagcaaag gttgtacatc
     7561 tatttttaga ttaagatatc taatattagc agagcattaa cttggaactt taacttatga
     7621 tttgaataaa ttttcacatt aaaaaacgag acagtctatt agttaaaaat agatgatgtc
     7681 tcgtttactt tatgatgcta tagcaaatta attatgttta ggctttgatg gacaatcttg
     7741 taaatgtgca tcatataaac cggctgcttc taaaaacgaa aatactgtta ctggacctaa
     7801 aaatttaaac ccgtattgtt ttaaatcttt agatagttgt gttgcagtat catcaactgt
     7861 gatacgatca gaagcatgtt catactgcaa atctttaggc ttaccattta catatgacca
     7921 taaaaattta ctaaaactac catatgcttg ttcaattttt aaataccctt gagcttgatt
     7981 aacaattgct tctaattttt tacgatgatg aacgatattt ggaaaagtca ttaagcggtc
     8041 gatatcttga gcggtcattt gtgctacctt ttctggttcg aaatcataaa atgcttcttc
     8101 ataggcttct ttctttttta aaatagttaa ccaagatagc ccagcatgtt gtgattctaa
     8161 tgctaaaagt ttaaacaatg ccttgctatc atagagcggt tgtccccata catgatcatg
     8221 atagtctaag tagactggat ctttagtacc aaatgcgcat tcattcattt tattcccacc
     8281 tatacattga cttattttgt caatcattat actataagtt aagagtttaa tataggattt
     8341 gggaagagta agttcaaatt attttaaaga gagcatatga tttggtgtga gtatgtaaat
     8401 gatttttact gaaggtagcc cacagagtat cttagctgaa cgtgattaat agtaggctaa
     8461 gacgtgtttg agcgttaatc ataatctaga gtgcttgaag agatgaagca tttcaaattt
     8521 cacgatgata gtattggtat gattattcca attatgttaa aagagtgaat ttgatattaa
     8581 tcatccttct tgaatatagg tggtaccacg gaatatccgt cctatttgta tataggatgg
     8641 atatttttat ttttttagga ggtatagcaa atggaaatga aaccaaaata tgatcctcgt
     8701 gaagttgaag cgggacgtta tgaagaatgg gtaaagaatg gttattttaa accgtcagaa
     8761 gataaatcaa aagaaacata tacaattgtt atcccgccac caaatgtaac tggtaaatta
     8821 catttaggac atgcatggga tacgacttta caagatatca ttacacgtat gaaacgtatg
     8881 caaggatacg atacgttata cttaccaggt atggatcatg ctggtattgc gacacaggca
     8941 aaggtagaag ctaaattaaa tgaacaagga ataactagat atgatcttgg tcgtgaaaag
     9001 tttttagaac aggcatggga ttggaaagaa gagtatgcgt catttattcg tgcgcaatgg
     9061 gctaaattag gtctaggttt agattatagt agagaacgtt ttactttaga tgaaggttta
     9121 agtaaagcag ttaaaaaagt ttttgttgat ttatacaata aaggaattat ttatcgtggc
     9181 gaacgtatta taaattggga tcctaaagca cgtacagctt tatctgatat tgaagtaata
     9241 catgaagatg ttcaaggtgc gttttatcat tttaaatatc cttacgctga tggtgaaggt
     9301 tttattgaaa ttgcaacaac aagaccagaa acgatgttag gtgatacagc gattgttgtt
     9361 aaccctaatg acgaacgata caaagatgta atcggtaaaa ctgttatatt accaatcgta
     9421 ggacgcgaac tgcctatttt agcagatgag tatgttgata tagacttcgg ttctggtgct
     9481 atgaaagtga caccagcaca tgaccctaat gattttgaaa ttggtcaaag acatcaatta
     9541 gaaaatatta tcgttatgga tgaaaatggt aaaatgaacg acaaagcggg taaatatgaa
     9601 ggtatggacc gttttgattg tcgtaaacag ctagttaaag atttaaaaga acaagattta
     9661 gttatcaaga ttgaagatca tgttcattct gtaggtcatt cagaacgatc tggcgctgtt
     9721 gttgaaccat atttatcaac acaatggttt gtgcgcatgg aagacttagc gaaacgttca
     9781 ttagataacc aaaaaacaga tgatcgtatt gatttttatc cgcaacgttt cgaacataca
     9841 tttaaccaat ggatggaaaa tattagagat tggacgattt caagacaatt atggtggggt
     9901 catcaaattc cggcttggta tcataaagaa acaggcgaaa tatatgttgg agaagaagcg
     9961 ccaactgata ttgaaaattg gcaacaagat gaagatgtat tagatacgtg gttctcaagt
    10021 gctttatggc ctttctctac gttaggttgg cctgatttag aaagtgaaga ctttaaacga
    10081 tactacccaa caaatgcctt agttacaggt tacgatatta tctttttctg ggtagcacgc
    10141 atgatattcc aaggcttaga atttacagat cgtcgtccat ttaatgatgt attattacac
    10201 ggtttagttc gtgctgaaga cgggcgtaag atgagtaaat cattaggtaa tggtgtggat
    10261 ccaatggatg ttattgacga atacggtgct gatagcttgc gttacttctt agcaacaggt
    10321 tcatctccag gacatgattt aagatactca actgaaaaag ttgagtcagt gtggaacttt
    10381 atcaataaaa tctggaatgg ggcacgtttc agtttaatga atatcggtga agactttaaa
    10441 gttgaagata tcgatttaag tggtaactta tcattagcag ataaatggat tctaacacgt
    10501 ttaaatgaaa cgattgcaac agttactgat ttaagtgaca aatatgaatt cggcgaagtt
    10561 ggacgtgcat tatataattt catttgggat gatttctgtg attggtacat tgaaatgagt
    10621 aaaattccaa tgaatagtaa tgatgaagaa caaaaacaag ttacacgttc agtattgagt
    10681 tatactttag acaatattat gagaatgcta catccattca tgccatttgt aacagagaaa
    10741 atatggcaaa gtttaccaca tgaaggtgac acaattgtta aagcttcatg gccagaagtg
    10801 cgtgaatcat tgatttttga agaaagtaaa caaacaatgc aacaacttgt tgaaatcatt
    10861 aaatctgtaa gacaatcacg tgtagaagta aatacgccat tgtctaaaga aatacctatt
    10921 ttaattcaag ctaaagataa agaaattgaa acaactttat cacaaaacaa agattattta
    10981 atcaaattct gtaatcctag taccttaaat attagcactg acgtggaaat tcctgagaaa
    11041 gcaatgacat cagttgtaat tgcaggtaaa gtggtattac cattagaagg gctaattgat
    11101 atggataagg aaatcagccg tttggaaaaa gaattagcta aacttcaaag cgaattagat
    11161 agagtagata aaaagctctc taatgaaaac tttgtaagta aagcacctga aaaggttata
    11221 aatgaagaaa aacgtaaaaa acaagattat caagaaaaat atgatggtgt gaaggcaaga
    11281 attgaacaat taaaagcata ggagttagta acaatgaatt acctagagag cttgtattgg
    11341 atacacgaaa gaactaaatt tggcatcaaa ccaggtgtta aacgtatgga atggatgcta
    11401 gcacaattta ataatcctca aaataacatt aagggtattc atgtaggtgg cacaaatggt
    11461 aaaggctcta cagttgctta ccttagaaca gctttagttg aaaatggtta tgaagtaggt
    11521 acatttacgt cgccgtttat tgaaacattt aatgaacgaa ttagtctaaa tggtgtgcca
    11581 atatcaaatg acgctattgt agaattagta tcacgtatta aaccagtaag tgaaatgatg
    11641 gaacgtgaaa cagatttagg tgttgcaact gaattcgaaa taatcacagc gatgatgttt
    11701 ttatattttg gtgaaataca tcctgttgat tttgtcattg ttgaggctgg attgggtata
    11761 aagaacgatt cgacaaatgt ctttacaccg gttttatcaa tcttaactag tatcggtcta
    11821 gaccatacag atattttagg tggtacttat ctagatattg ctagggataa aggcgcgatt
    11881 ataaagccta acgttccagt gatatatgct gttaaaaatg aagatgcatt aaaatatgtt
    11941 cgtgaacgcg caattgaaca acatgtaaag ccaattgaat tagatagaga aattgttgtt
    12001 gtatcgcaaa atgatgaatt tacttaccgt tataaagatt atgaattaga aacaatcatt
    12061 ttaagcatgt taggtgaaca tcagaaacaa aatgctgcat tagccataac agctcttatt
    12121 gaattaaatg aacaaggatt aattgaatta gatttcaata agatgataga cggtattgaa
    12181 tcagttcgtt ggactggacg tattgagcag gtgcatgaca aacctttaat cattttggat
    12241 ggcgcacata attcagagag tatagatgct ctaattgata caattaaaca gtaccatgat
    12301 aaagaaaaag tagatatttt gttctcagca ataaacggaa aaccgattaa cgagatggtc
    12361 aaacatttaa gtttaattgc gcatacgttt tatgcaactg aatttgattt tccgaaagcg
    12421 ttacgcaaag aagaaattgt aggtagtatt gaaaatgatg aaatacaatt agtagatgac
    12481 tacgttgaat ttataaaaaa ttatcaaggt gatacattag taattaccgg tagtctgtat
    12541 ttcataagtg aagttaaatc aaaattagac tttgaagcat tataaaacta aaactaaaat
    12601 agccatgaag aatctattta atcaataggt agagcttcat ggttattttt tatgctctaa
    12661 atcgtatgtg tttataaggt tcaatttctt gccattatta attttgccga gcagtaatgg
    12721 gttgttaaag tatccatcaa ttatgcttag caatattgaa aaatttaaat gaaaaagcgg
    12781 ttcaattctt gaaaaatagt gtatatttac attttggtaa taactaacta taatttacat
    12841 aaggaggttg tcatttggta gtattgttat cttatagttg cagttgtatt tttagttttc
    12901 tatatcaatt tatatctata gaagaaacgt catttgatta tttacataga agatcgaaat
    12961 gtgattattg taattcgtca ctcaaatggt atgaattaat gccgattatt agttttttat
    13021 tattaaaagg gcgatgtcga aactgtcgaa agcgtatttc cctaacacat ttcttagggg
    13081 aaacctttgc tttaatacct atcgtcttta ttaagtatga tttcacatac gtaaatgcta
    13141 cgctatttat aactacatat gtttttctgc ttatttttac tatgaccgat atcacttctt
    13201 taatgttaga ttgtcgctta attataattt attgtatcgt ttctctctcg ttaagtatga
    13261 tttatccagt agcttttatc attattagta tgaccacgca tatattctac tttttatttc
    13321 gggcatatat tggttatggt gacgttttac taatatctgc actttctttg tttttccctc
    13381 tccaattcac tatttatgtc attttattta catttgtcat tgctggttta gttgctttaa
    13441 ttaccatgat atttaagccg attaaactat taccccttgt tccatttata tttatttcct
    13501 tttttatcaa ttcacttttt tataatgata tccatcaatt tttaggaggc gtatattttt
    13561 gaaaattaaa gaaatggtaa cttcagaaat gccaagagaa cgtttgttaa gccatggtgc
    13621 aaaaagtctt tcgaatacag aattattagc tatattaatt aacaccggaa gaaaaggatt
    13681 ctcgagcata gacattagta atgaactgct taaatctgct tcgaatctga atgaattgaa
    13741 aaaatcttca attaacgatt tgataaaagt taaagggatt ggattacaaa aagcaattac
    13801 tttaaaagca gcatttgagt taggggaaag gatggggaga agagctgaaa ataatcgtat
    13861 aaaaataacg caaccaagtg atgttgctga ttatatgatt ccaacaatga aagatttaac
    13921 acaagaacat tttgtcattt tattgttaaa ttcaaaaaat gtagtgatta aagaaacctg
    13981 tgtttttaaa ggtacattaa atagttcgat tgtacatcca cgtgaaattt ttagtattgc
    14041 ggtgagagaa aatgccaatg caatcatcgc agttcataat catccatccg gtgatgtaac
    14101 gccctcacaa gaagatatca taacaacaat gaggttgaag gagtgtggtt tgattttagg
    14161 gatagattta ttggatcata ttataatcgg tgataataga tttaccagtc ttgtagaagc
    14221 gggttacttt gatgaaaatg attgaagttg atagaattaa tgacgtatct tgtgtataat
    14281 acctacgaag tactttcatt ggaggaaaaa tagtgactct atttattatt atcggggttc
    14341 tcgtgccaat ggtttatacc atgcagttaa atattaaaaa tgaacctgta acaaagcgca
    14401 atcttttaat aacattagct ttatctacgt taggtatttt agtaaccgcg ttagcaggtg
    14461 taatcgttac gaaacaagct tttcctttat taagtgtagc aattggctca atttttactg
    14521 gaatcgtttg gggcctttta ctaagtggta gctacgcgct gatacgattt ttatctaacg
    14581 catttgggcg taagtaatag taatctgttc atcaagtagt atccgtgctt gaaaacaaac
    14641 taaaactcct aatgtggaac tagttaaaaa attctaaact agtacattag gagttttgtt
    14701 atgcagaata aatttaattg ttaaattgaa agtgcggtaa aaatccacta tttatttgaa
    14761 aaaaatcgag caaccaaatt aaaccttgat atcctaagta aatacataat aaaacaagtc
    14821 cgacataaat taaaaatcgc aaaatagaaa gtccaactct aaaaaggagg atgactaata
    14881 gtgctattaa aataattgtt agtatactca atgctacaaa cctcctaata cgcttttaaa
    14941 tcataacaaa attataagct attttaaatg aacgtgcatc agtcctaaga tatatcatga
    15001 ttaaggctga agactgagga aaatatacat aataaaatat ataattagat gaagacattg
    15061 gaggcgaact atgagattga tatttagtat gattaaaaac attatagcag taatagcaat
    15121 tgtgataatt gtttatatcg ccttgaaata cgcacctttt ttaaaagaac aagattggaa
    15181 tcctgtaaat cacgataatg atcaaatgaa tcaagttacg caacccacta atgatgccca
    15241 acatgtttat gtaagtggtg agaaatattc attaaaagaa aatgatttaa taaagaatgt
    15301 accattaagc caaattaaaa atgtatttaa aatgatagat aaacaagagt ttatggctgt
    15361 gtctggaatg aatcgcatgg cttataatga tcaatatatt ataggtcaaa gaggagacga
    15421 atttattctt tataaatttg gagatgagtc aatgcgtgtt tacaatactg aatttgaaat
    15481 gcaacaagac ttaaatgaat tagggcaaaa tttacaacta aaatctgaaa atgcttatca
    15541 ataggaaatt attgcataag aaatatattt tagttatcct gtcactagaa taaatttaaa
    15601 aggttggtgt agttcaaatg gcagaacaat caaaacagaa acaagctaat gaacaacaaa
    15661 aggcccagaa cttattcgca cgttggagac aacttcagaa ttcgaacagt gaatcctcga
    15721 acgatacaaa caaataattt taaaatttaa atcgattatt taaactacta gtgactggaa
    15781 tgtttgctag tagttttttt gtttttaaaa tgccacaaat tttttacgga gttgttcatt
    15841 ttcaaagtaa ttcgtagtta gtgctttatt tcataatggg agtaatataa aatagaaaag
    15901 gatatacaat attttgacat aatagaggtg ttctgggtgc ttaagttttt taaaaataac
    15961 aaattaattg ttgttttatg tgcaattatc gtttttattg cattaattgg gctgtccata
    16021 cgttcacaat ctcaatcacc tcctgaacaa tatataggtg attctgtgtc ttttggacaa
    16081 cgagttgtga gttatccagt taattttgtt gctggtacga ttggggactt ttttaaaaaa
    16141 ggagattcta aagaatctaa aaataagatt agccagttag aatctaagaa ccaacaatta
    16201 gaagcggaaa atgaaaaatt aaaaaaagag cttgatttaa aagatatttc aaaatttgat
    16261 cctatttcta ctacggtttt ggcaagaaat ccggatcagt ggatgaatac aattgtaatt
    16321 gataagggat ctaaatcagg tataacttca aatatggctg tgatgacatc acaaggtttt
    16381 gttggaagag ttactaaagt taataaattt tcttcacaag ttgatttaat ctcaactaat
    16441 acacgtgcgg gtaaattatc tgtaaatata caacacggtt ctaaaaatat atttggttta
    16501 attgatcgtt atgatgaaaa gaactcagaa cttgtaatta gtgacattaa taatagagat
    16561 aatatctcaa aaggtgataa agtcgttaca agtggattag ctgatcaact accaagtaat
    16621 ttatatatag gagaagtgac taaggttcaa aatgatcaat acggcttagc taaagaagtt
    16681 agggttaaga ctggtgcgga cttaacagat ttgagtcatg tttatgttgc aaaaagagat
    16741 cctaaaacaa ttcctgatga tgaaagcagg gataaataat gcgtacactg tattattttt
    16801 tgataggtat tttactattt tatatagata ctgcaattgg gcttcttatt ccaatgcaca
    16861 taggtaagtt tgaacttgta tttgtaccac accttacatt tatgtatatt ttaatgatgg
    16921 tagtgtatcg tggctttggc gtatcattat tgctcagcat atttttaggc gtaatgactg
    16981 atgtatactt tggtagtatt tatggagtgt acttatttgg ctatatattg tttttagcac
    17041 ttatagatcg attctttaaa attttttaca aagatcattc gatgttattc attattatat
    17101 tagccagcac cttattatta gaagtctatg tggcattaat atacggtatg ttaggattca
    17161 ttcaatttga tattattcat tttgtagtct ttagattatt gccaacatta attatgaatt
    17221 ttgtactgtt gattatgctt tatccgttga ttataaagtt ccttaaaaaa acaaacaatg
    17281 acattgacat gaaacgtcgt caatggtaag atgcaatagt tgagtagtta taagtagcta
    17341 catacaaccg ctcaaatata ggttcaagaa catttttaat gaagaaagcg attttcccac
    17401 agagtgcaaa cgcttgactc aataacgaaa tgtcacctat gtatggcgtg acttattata
    17461 caggaggtgc aaagtatgtt tgctattatt gaaacaggtg gaaaacaaat caaagtagaa
    17521 gaaggtcaag aaatcttcgt tgaaaaatta gacgtaaacg aaggagatac ttttacattt
    17581 gataaagtat tatttgtagg tggagattca gttaaagttg gagcgccaac agttgaaggt
    17641 gcaacagtta ctgctactgt taataaacaa ggtcgcggta aaaaaatcac tgtattcaca
    17701 tacaaacgtc gtaaaaattc aaaacgtaaa aaaggccatc gtcaaccata cactaaatta
    17761 acaatcgata aaatcaacgc gtaattatta tgattactgt tgatattaca gttaatgatg
    17821 aaggcaaagt aacagacgtt attatggatg gccatgctga ccatggtgaa tatggtcatg
    17881 atatcgtttg tgctggagct tcagctgtat tgtttggtag tgttaatgcg attataggat
    17941 tgacatctga gagaccagat atcaattatg acgacaatgg tggtcatttt catataagaa
    18001 gcgttgatac aaacaacgat gaagcgcaac taattcttca aacaatgctt gtgtctttac
    18061 aaactattga agaagaatat aatgagaata ttagattaaa ttataagtga ggtgcattcc
    18121 gatgttaaaa ttaaacttac aattcttcgc atctaaaaaa ggggtaagtt ctacaaaaaa
    18181 cggacgtgac tctgaatcaa aacgcttagg tgctaaacgt gctgacggtc aattcgtaac
    18241 aggtggttca attttatatc gccaacgtgg tactaaaatt taccctggtg aaaatgtagg
    18301 tcgtggtggc gatgatacat tattcgctaa aatcgacggc gttgttaaat tcgaacgtaa
    18361 aggtcgcgac aaaaaacaag tttctgtata tgcagtagct gaataatttt gtctagttaa
    18421 caccagaagt gaatcttctg gtgtttttta ctttttataa aatgtttttc attattattt
    18481 tcattatgtt attaaaaaat gggaatttta gacgttatac taaatgtgca actgtataag
    18541 agcccctaat cactaaacta taagggggac aaaggaatac agttgcagcg tttaaagaat
    18601 aaactgtacc acaattggtg ctgagaaata taagtatttt aaagcaaaga tttataaaag
    18661 taactgcata agagccccta attatttaca atatataagg ggctctaata tgctataatt
    18721 attgggaaaa tgaaaattat atgtaaaaga ggtgagatat atgtttgtcg atcaagtcaa
    18781 aatatctctt aaagccggtg atggtggtaa tggtattacc gcatacagaa gagaaaaata
    18841 tgtaccattt ggtggaccag ctggcggtga cggtggtaaa ggtgcttcag tcgtatttga
    18901 agtggatgaa ggtttaagaa cgttattaga ttttagatat caacgtcatt ttaaagcaag
    18961 caaaggtgaa aatggccaaa gtagtaatat gcatggtaaa aatgcggaag atttagtatt
    19021 aaaagttcca cctggtacaa ttattaaaaa tgttgaaaca gacgaagtgt tagcagatct
    19081 tgttgaagat ggtcaaagag ctgtagtagc gaagggcggt cgaggtggcc gaggtaattc
    19141 acgttttgca acacctagaa accctgcacc tgacttcagt gaaaaaggtg aaccaggtga
    19201 ggaattagat gtatctttag aattgaaatt attagctgat gtaggattag taggtttccc
    19261 tagtgtgggt aaatcgactt tattatctat cgtttcaaaa gctaagccta aaattggggc
    19321 atatcatttt acaacgatta aaccaaatct aggtgttgtt tcaacgcctg atcaacgtag
    19381 ttttgttatg gcagatttac caggtttaat tgaaggtgca tctgatggcg ttggattagg
    19441 acatcaattt ttaagacatg tagagagaac aaaagttatt gttcacatga ttgatatgag
    19501 cggttctgaa ggtagagaac ctattgaaga ttataaagtc attaatcaag aattagctgc
    19561 gtacgagcaa cgtttagaag atagacctca aatcgtagta gctaacaaga tggatttacc
    19621 tgaatcacaa gataatttaa acttgtttaa agaagaaatt ggcgaagatg tgccagttat
    19681 tccagtttca acaataacgc gtgataatat tgatcaatta ttatatgcaa tagcagataa
    19741 attagaagaa tataaagatg ttgacttcac agttgaagaa gaggagtcag ttggcattaa
    19801 ccgagtatta tataaacata caccgtcaca agataaattt acaatttcaa gagatgatga
    19861 tggtgcttat gtggtaagtg gtaatgctat tgaaagaatg tttaaaatga ctgactttaa
    19921 cagtgatcca gcagtacgtc gatttgctcg tcaaatgcgt tcgatgggta ttgatgatgc
    19981 gcttagagaa cgtggttgta aaaatggtga tatcgttaga attcttggcg gagaatttga
    20041 attcgttgaa taggagcgaa acatgatgga caataaagat tataaaaagt tttatttaat
    20101 tagagaagat gtcttgcctg aatccgtggt taaaacattg aagattaaag atgccttaaa
    20161 aagtgatccg acattgtcca tttatgatgc cgttaaacag tttgatctat ctagaagtgc
    20221 tttttataaa tatagagaaa cgatatttcc agtagacgat aaaatgcttg accatcgaga
    20281 atttacatta attttatatg taactgatat tgttggtatg ttggcacgtg tactagatgt
    20341 tatatcaaag ttagaactat ctgtattaac gattcatcaa agtattccaa tggaagaaaa
    20401 agcaacaata acattatcac tgaatgctaa atctaaagaa acttcagtag aagatgttat
    20461 tggcgctttg agaaatttag attatgtatc aaaagtagaa ttaattagta tgagtatgta
    20521 aggatgtgcc tataatgtac gcgtatgtca aaggtaagtt aacacattta tatcctacac
    20581 acgtagttgt tgaaactgct ggtgttggtt atgaaattca aacaccaaat tcttatcgtt
    20641 ttcaaaagca tctagatcat gaagttttaa ttcatacatc tttaattgtt cgtgaagatg
    20701 cacaattatt gtatggattt agtagtgaag aagagaaaga tatgttcttg agtttaatta
    20761 aagttactgg tattggtccg aaatcagctt tagctatttt agcgacaagt acgcctaatg
    20821 aagtaaaacg tgccattgaa aatgaaaatg atacgtattt aactaaattc ccaggaattg
    20881 gtaagaaaac ggcaagacag attgtcttag atttaaaagg taaagtgaaa attactgaag
    20941 aagatagcga ttcattatta caagtagacg ctacttcgac ggtacaagat caattcgtgc
    21001 aagaagcaat gttagcgtta gaagcattag gttattctaa acgagagctt gcaaaagttg
    21061 agaaaacgtt aaataaaaat aaatatgact cagttgatga agctgttaag gcaggtcttc
    21121 aattagttgt atcttaattt taaatagatt aataggggaa gtgttgtcat gaatgagcgt
    21181 atggttgatc aatcaatgca tagtgaagaa actgatttcg aattgtcgct tagacctacg
    21241 agattacgac aatatattgg tcaaaattca ataaaaagta atttagaagt atttattaaa
    21301 gcggctaaac ttcgtcatga accattagat catgtattgc tttttggccc ccctggatta
    21361 ggtaagacaa cattatctaa tatcattgcc aatgaaatgg aagttaatat acgtacagta
    21421 tcagggcctt cattagaaag acctggtgat ttggctgcaa ttttatcagg acttcaacct
    21481 ggagatgttt tgtttattga tgaaatacac agactgagta gtgttgttga agaagtgtta
    21541 taccctgcaa tggaagattt ctttttagat attatcattg gtaaaggcga tgaggctaga
    21601 agtatccgta tcgacttacc tccattcact ttggtaggtg caacaacgcg agctggcagc
    21661 ttaacaggtc cactaaggga tcgatttggt gtgcacttaa gattagaata ttataacgaa
    21721 tcagatttaa aagaaatcat tattagaaca gctgaggttt taggcacagg tattgatgaa
    21781 gaaagtgcca ttgaacttgc taaacgttct agagggactc caagagtagc aaatcgacta
    21841 ttgaagcggg taagagactt ccagcaagtg aatgaagatg aacaaatata cattgaaaca
    21901 acgaagcacg cattaggttt acttcaagtt gatcaacacg gactagatta cattgatcat
    21961 aaaatgatga actgtattat taagcagtat aatggcggac ctgttggttt agatacgatt
    22021 gccgtaacaa ttggtgaaga acgtattaca attgaggacg tttatgagcc atttcttatt
    22081 cagaaaggct ttttagaacg tacgccacgt ggcagaaaag caacaccatt agcttatgaa
    22141 cattttgcaa agtcgaatga ggagagagaa taacgtgaat attgaagaat ttgactatga
    22201 cttaccagaa tcattaattg ctcaaacgcc tttaaaagat cgtgatcata gtcgtttatt
    22261 agtcatggat agagaaactg gtgaaatgaa acatttacat ttcaaagata tcattgagta
    22321 ttttagacct ggtgatacat tagtgcttaa cgatacgcga gtaatgccag ctagactttt
    22381 tggtttaaaa gaagaaactg gtgcaaaagt tgaaatgtta atgttaactc aaattgaagg
    22441 taatgattgg gaagtcttac tgaaaccagc taagcgtatt aaagttggta ataaattgaa
    22501 ttttggtaat ggcaaaatta tagctgaatg cataaaagaa atggatcaag gtggacgcat
    22561 catgcgttta cattatgaag gtattttaca agaaagatta gatgaattag gggaaatgcc
    22621 actgccacca tacatcaaag aacgtttaga tgatccagat cgttatcaaa cagtttacgc
    22681 taaagaaagt ggttcagcgg cagcaccaac agcaggatta cattttactg atgagttatt
    22741 aattgaaatt aaaaataaag gtgttaatat cgcatttgtt acattacatg ttgggttagg
    22801 tacgtttaga ccggtgagcg tagacgatgt gaatgaccac gaaatgcata gtgaatatta
    22861 tcaaatgaca caagaaacag ctgatttatt aaatgatact aagtccaaag gacatcgcat
    22921 tatatcagtt ggtacaactt caacacgtac acttgaaaca attcgacgcg atcatgataa
    22981 atttgttgaa acgagtggct ggactaatat atttatttat ccaggatttg attttaaagc
    23041 aattgatggc cagattacta attttcattt accaaaatca acattagtta tgctagtatc
    23101 agcgtttagt agtcgtgaaa atgttctgaa tgcttataaa acggcagtaa atttagaata
    23161 tagattcttt agttttggcg atgcaatgtt aattatataa aaagaatgtg aggattttga
    23221 atatgcctgc agtaacatac gaacacatta aaacttgtaa acaatcaggt gcgcgtttag
    23281 gtatcgtgca cacaccacac ggttcatttg aaacacctat gtttatgcca gttggtacta
    23341 aagcaaccgt taaaacaatg agtccagaag agttaagaca aattgaagca aaaatcattt
    23401 tgggcaacac atatcatttg tggttacaac ccggaaatga tattatcaaa cacgctgggg
    23461 gattacataa attcatgaat tgggatggtc cgattcttac agattcaggc ggtttccaag
    23521 tgtttagttt aagtaattta cgtaaaatta cagaagaagg cgtggaattt agacatcata
    23581 ctaatgggtc taaattattt ttgagtcctg agaaatcaat gcaaattcaa aatgatttag
    23641 gatctgatat tatgatggca tttgatgaat gtccaccgat gcctgctgaa tatgattatg
    23701 taaaaaaatc tattgaacgt acaacacgtt gggcgaaaag atgtctagat gcacaccaaa
    23761 gacctgaaga tcaagcattg ttcggcatta tacaaggtgg cgaatatgaa gatttaagag
    23821 aacaaagtgc aaaggattta gtagaattag attttcctgg ttatgcaatc ggcggtttgt
    23881 cagttggtga acctaaaccg gttatgtata aaatggttga acatacagag cagtttatgc
    23941 ctaaagataa accaagatat ttaatgggtg taggatctcc agatgcgtta atcgaatgta
    24001 gtattcgcgg catggatatg tttgattgtg tcttaccgac acgtattgcc agaaatggta
    24061 cttgtatgac atcgcaaggt cgtttagtta ttaaaaatgc aaaatttgca gatgatttaa
    24121 gaccgttaga tgagaattgt gactgttata catgtcaaaa ctattcaaga gcgtatatac
    24181 gtcatttaat caaggcagag gaaacttttg gtattcgtct tactactatt cataatttac
    24241 attttctgct aaaattaatg gaagatataa gacaagccat tcgagaagat cgtcttttag
    24301 atttcaaaga agaattcttc gagcaatatg gattaaatgt tgagaaccca aaaaactttt
    24361 aagcaagagg agcgtataaa atgcaatttt cattactaat atatatagtc gtaatttttg
    24421 cggttatgta tttcttgatg atcagaccac aacaaaaacg tgcgaaacag catcgtgagt
    24481 tgattaataa cattcaatct ggtcaaagaa ttacaactat tggtggtatt aaaggtactg
    24541 ttaaagcagt agatgaaaca actgttgtta ttacagttaa tggtcatggt actgaattaa
    24601 ctttcgaaaa acctgctatt aaacaagttg acccttcata atgagaagtg gtattaaaaa
    24661 ggatgaaacg aatagcgttt catccttttt aagttataag tagtatgtag aatagcttat
    24721 ttttattcca aaaaaagaaa ttgtgataca taataattgt taaatttaaa ttaaatatat
    24781 ctatgactat ttttactccc tgctctagta tgttaatatg tataaggcta ataaattttg
    24841 taagaaagat ttaacttgcg ttataaaata gtattcaagt attctaaata catacgtaaa
    24901 taatgaggtg ttcatgtgaa gaaaagtagt agaataattg cgttcttgct tttagtcgta
    24961 ttgttgtttg ccggaatggc tgcaacttat aaaagtgtta taaagaatgt caatttagga
    25021 cttgatttgc aaggtggatt tgaggtgctt tatcaagtcg atcctttaaa taaaggcgac
    25081 aaaatagata aaaaagcctt acaatcaaca gcgcaaacat tagaaaatcg tgttaacgtt
    25141 ttaggtgttt cggaacctaa aattcaagtt gaagaaccta atagaattag agtacaactt
    25201 gctggtgtaa cagaccagaa tgaagcacgt aaaattttat cttctcaagc taatttaaca
    25261 attcgcgatg cagaagataa agtaaaatta agcggttctg atattaaaca gggatctgcc
    25321 aaacaagaat ttaaacagga aacaaaccag cctacagtta cttttaaagt aaaagacaaa
    25381 aacaaattta aaaaagtaac ggaagaaatc tctaagaaac gtgataatgt catggttgtt
    25441 tggttagatt tcaaaaaagg tgacagctat aaaaaagaag cacaaaagaa aaaccctaaa
    25501 tttatttctg cagcaagtgt tgatcaacca atcaattctg atagcgtaga aatctcgggt
    25561 ggatttaaag gacaagaagg cgttaaaaaa gcaaaacaaa tcgccgaatt gttaaatgca
    25621 ggttctttac cagttgactt gaaagaaatt tattcaaact cagtaggtgc ccaatttggt
    25681 caagatgcat tagataaaac tgtgtttgca tcgtttattg gtgtagcatt aatttattta
    25741 ttcatgcttg gattctaccg attacctggt ttagtagcga ttattgcatt gacaacttat
    25801 atctatctaa cgttagtagc atttaatttc atttccgggg ttctaacttt accaggatta
    25861 gcggcgttgg tattaggtgt aggtatggct gtagatgcca atattatcat gtatgagcgt
    25921 attaaggatg aacttcgaat aggtagaacg ataaagcaag ccttttctaa agcaaacaaa
    25981 agttcattcc taacaatttt tgattctaac ttaacaacag ttatcgccgc agcagtatta
    26041 ttcttcttcg gtgaaagttc agttaaaggt ttcgcgacaa tgttattatt aggtattcta
    26101 atgatctttg ttacagccgt gttcttatca agattcttat tatcattact tgtttcatca
    26161 aatatattca aaaatcaatt ttggttattt ggtgttaaaa agaataaacg acatgatatt
    26221 aatgaaggtg tagatgttca tgaccttaaa acttcattcg agaaatggaa ttttgttaaa
    26281 ttagctaagc cattaattgg agtaagtatt ttaattgtag ttgtcggttt agttattctt
    26341 tatatcttca agttaaactt aggaattgat ttctcaagtg gtacacgcgt agatttccaa
    26401 tctaaacaag caattactca acaaaaggta gagcaagttg taaaagatag tggacttaaa
    26461 gcggatcaaa ttcagatcaa tggtaaagac aataaagtag caactgttca atttaaagac
    26521 gatttaacgc gtgctcaaga taataaattg agtgataata ttaaatcgaa attcggtgac
    26581 acacctcaaa ttaacactgt atcaccaatt attggacagg aattagcaaa aaatgcaatg
    26641 cttgcattaa tctatgcatc gataggtatc atcatctatg tatcattacg atttgaatgg
    26701 cgcatgggtc tttcatctgt attggcatta ttacatgatg tattcattat agtagcgatt
    26761 ttcagtttat ttagaattga agtagattta acatttatcg ccgctgtatt aacaattgtc
    26821 ggttattcaa ttaatgatac aatcgtaacg tttgaccgtg tacgtgaaaa cttacaaaag
    26881 gttaaagtga ttacgacaac agaacaaatt gatgatatcg ttaatagatc aattagacag
    26941 acaatgacac gttcaattaa tacagtatta acagttattg tagtagtagt tgctatacta
    27001 ttcttcggtg ctcctacgat attcaacttt actttagcat tatttatcgg attgatttct
    27061 ggtgtattct cttcaatctt cattgccgtt ccgctatggg gaataatgaa aaaacgtcag
    27121 ttgaaaaaat cgccgaaaca caaattagtt gtatataaag aaaagaaatc gaacgatgaa
    27181 aagattttag tttaaaatga attaagcggt atgtgaaaca ataaagagtt cacataccgc
    27241 tttttacttt atttaa
//
LOCUS       DALAGF010000026        27220 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.026, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000026 DALAGF010000000
VERSION     DALAGF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 27220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27220
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.026"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..256)
                     /locus_tag="O2692_001049"
     CDS             complement(<1..256)
                     /locus_tag="O2692_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HCZ4298686.1"
                     /translation="MKFSTLSEEEFTNYTKKHFKHYTQSIELYNYRNKINHEAHIVGV
                     KNDKNEVIAACLLTEARIFKFYKYFYSHRGPLLDYFDAKLV"
     gene            complement(284..1402)
                     /locus_tag="O2692_001050"
     CDS             complement(284..1402)
                     /locus_tag="O2692_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCZ4298687.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSTISNGNEINQD
                     IKISNTPKNSSQGNNLVINNNELTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     KTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
     gene            complement(1629..2537)
                     /gene="sucD"
                     /locus_tag="O2692_001051"
     CDS             complement(1629..2537)
                     /gene="sucD"
                     /locus_tag="O2692_001051"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCZ4298688.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
     gene            complement(2559..3725)
                     /gene="sucC"
                     /locus_tag="O2692_001052"
     CDS             complement(2559..3725)
                     /gene="sucC"
                     /locus_tag="O2692_001052"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCZ4298689.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            complement(3834..4601)
                     /locus_tag="O2692_001053"
     CDS             complement(3834..4601)
                     /locus_tag="O2692_001053"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCZ4298690.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            complement(4585..5469)
                     /gene="ylqF"
                     /locus_tag="O2692_001054"
     CDS             complement(4585..5469)
                     /gene="ylqF"
                     /locus_tag="O2692_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCZ4298691.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            5870..8476
                     /gene="yfhO"
                     /locus_tag="O2692_001055"
     CDS             5870..8476
                     /gene="yfhO"
                     /locus_tag="O2692_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HCZ4298692.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLRSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSANMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVCI
                     IAILYVLLSPTHPLALIVGIILLIVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGSFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKIKSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNIKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRVVTPVTIRIKAPDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKHGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            complement(8720..9070)
                     /gene="rplS"
                     /locus_tag="O2692_001056"
     CDS             complement(8720..9070)
                     /gene="rplS"
                     /locus_tag="O2692_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCZ4298693.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(9173..9910)
                     /gene="trmD"
                     /locus_tag="O2692_001057"
     CDS             complement(9173..9910)
                     /gene="trmD"
                     /locus_tag="O2692_001057"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCZ4298694.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            complement(9910..10413)
                     /gene="rimM"
                     /locus_tag="O2692_001058"
     CDS             complement(9910..10413)
                     /gene="rimM"
                     /locus_tag="O2692_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCZ4298695.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            complement(10601..10876)
                     /gene="rpsP"
                     /locus_tag="O2692_001059"
     CDS             complement(10601..10876)
                     /gene="rpsP"
                     /locus_tag="O2692_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCZ4298696.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            complement(11311..12678)
                     /gene="ffh"
                     /locus_tag="O2692_001060"
     CDS             complement(11311..12678)
                     /gene="ffh"
                     /locus_tag="O2692_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCZ4298697.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTQLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            complement(12704..13036)
                     /locus_tag="O2692_001061"
     CDS             complement(12704..13036)
                     /locus_tag="O2692_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCZ4298698.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            complement(13023..14273)
                     /gene="ftsY"
                     /locus_tag="O2692_001062"
     CDS             complement(13023..14273)
                     /gene="ftsY"
                     /locus_tag="O2692_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCZ4298699.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDA
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGSK"
     gene            complement(14273..17839)
                     /gene="smc"
                     /locus_tag="O2692_001063"
     CDS             complement(14273..17839)
                     /gene="smc"
                     /locus_tag="O2692_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCZ4298700.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIYGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETYLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQNQQTKHQLKGVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEAKLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            complement(17986..18717)
                     /locus_tag="O2692_001064"
     CDS             complement(17986..18717)
                     /locus_tag="O2692_001064"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCZ4298701.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            complement(18833..19066)
                     /locus_tag="O2692_001065"
     CDS             complement(18833..19066)
                     /locus_tag="O2692_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCZ4298702.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            complement(19309..20043)
                     /gene="fabG"
                     /locus_tag="O2692_001066"
     CDS             complement(19309..20043)
                     /gene="fabG"
                     /locus_tag="O2692_001066"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCZ4298703.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            complement(20036..20962)
                     /gene="fabD"
                     /locus_tag="O2692_001067"
     CDS             complement(20036..20962)
                     /gene="fabD"
                     /locus_tag="O2692_001067"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCZ4298704.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAANTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            complement(20955..21941)
                     /gene="plsX"
                     /locus_tag="O2692_001068"
     CDS             complement(20955..21941)
                     /gene="plsX"
                     /locus_tag="O2692_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCZ4298705.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNHDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            complement(21946..22518)
                     /gene="fapR"
                     /locus_tag="O2692_001069"
     CDS             complement(21946..22518)
                     /gene="fapR"
                     /locus_tag="O2692_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HCZ4298706.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            complement(22721..24781)
                     /gene="recG"
                     /locus_tag="O2692_001070"
     CDS             complement(22721..24781)
                     /gene="recG"
                     /locus_tag="O2692_001070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCZ4298707.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDIDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEINVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            complement(24971..26617)
                     /gene="fakA"
                     /locus_tag="O2692_001071"
     CDS             complement(24971..26617)
                     /gene="fakA"
                     /locus_tag="O2692_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HCZ4298708.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            complement(26632..27006)
                     /locus_tag="O2692_001072"
     CDS             complement(26632..27006)
                     /locus_tag="O2692_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCZ4298709.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
BASE COUNT         8140 a         5303 c         3635 g        10142 t
ORIGIN      
        1 aaactaattt agcatcgaaa taatcaagta aaggacctct atgagagtag aaatatttgt
       61 agaatttaaa aattcgtgcc tctgttaata aacatgcagc tataacttca tttttatcat
      121 tcttcactcc cacaatatgt gcttcatgat ttattttatt tctataatta tataattcta
      181 tagactgtgt ataatgtttg aagtgctttt tggtgtagtt ggtaaattct tcttcactta
      241 aagttgaaaa tttcatattt caatgtcctg ccttcgattt aaattatgct tttttaaatg
      301 gtctaataaa aatcatatca ttttcataat tatgaacaac tttatgtgca acctctgctt
      361 tacgccatcc accattattc cagttttgat ctaaactttg gaacttcatt aattttccat
      421 cataatcacc atttaagaca atagctgtat gaccatatcc tccaccaaat cttccactaa
      481 aaaccactaa gtccccaggt tcagctttga aagttggtgt gttgtgataa attttagctt
      541 cactattaaa attatttgca tatggtatat ctttagctcc atatcctttt aatccatgac
      601 cataaagatg attccaatat acattaacta aatcgaaaca ttgccatcca taactaccgt
      661 cgaaatccca tcctctattc tctaatgttt tcaaataatt taatgtactg ctattactat
      721 tacttttatt atttgaagac actgtttttg gttttggttc tactaatggt gtcattggca
      781 cttttaattt ttgaccaata aatattaaat taggatttgc tatattattt gtattttgaa
      841 tatttgaaac tgtagttttg tattttaatg ctatagcact aagtgtgtct ccttttttta
      901 cagtatagat ttgtgttttt ggagcttctc taaaactgta gtaaccaaag taattattag
      961 taactttatt cgttttcttc tgattagaat tttgagcttc caagtttgca attttaattt
     1021 ctttagtaag ttcattgtta ttaataacta gattgttacc ttggcttgag tttttcggag
     1081 tatttgaaat ctttatatct tgattaattt catttccgtt tgaaattgtt gatttgttgt
     1141 ctaactttaa acttgtgtcc gatgttttaa cagcaccttc attttttatt ttgtcttttg
     1201 tcgtattttt attagcattt aactctgatt tcgcgaatac attttgctca taccctcttg
     1261 taaaatcttt agatttatca atttcatctg catatgcttt gttcgacata cccaatgcca
     1321 aaaacatacc tattgaaatt gacaaaattc caatactaac ttttctaatt gaatagcgta
     1381 ctttactttg ttgtttattc atgatgaaac actccttaca ataatataca aacattataa
     1441 catagtgcgc tatatatttt aattaaaatt ttctttgttt tataaagcta ttgtaggagg
     1501 taaaataatt aacttgtctt tccaaaatat gaaaagtgta ctaaaattca tcgcacgaca
     1561 aatagcccat ttccgatact tttataaagt atggaatggg ctatagccat ttatatcatc
     1621 ttttaacttt atttattaac agttaataat gattcataaa tacctgcttc tttagcagct
     1681 tcaattaatg ttgaaccaat ttctgaaggt gttgccgctg ttttcacacc acaactattt
     1741 aatgttttaa ttttctcttc agcagtacct ttaccacctg aaatgattgc accagcatgt
     1801 cccatacgtt ttccaggagg tgctgtttgt ccaccgataa agcctacaac tggttttgtc
     1861 atattcgctt taatccattc agctgcttct tcttcagccg taccaccgat ttcaccaatc
     1921 ataacaactg ctttcgtttc gtcatcttca ttgaatgctt ttaaaacatc aataaagttt
     1981 gttccgttga ctgggtctcc accaatacca acagctgtag tttgaccaat accttcttca
     2041 gtcaattggt gcactgcttc atatgttaat gtacctgaac gagatactac accaacatga
     2101 ccttttttgt gaatatagcc aggcataata ccaattttac attcatctgc tgtaatcaca
     2161 cctggacagt tcggaccaac taaacgtgtt tttctacctt gtaagtagcg tttaacttta
     2221 accatgtcta atacaggaat atgttcagtg atacaaataa ccatatctaa gtctgcatca
     2281 gctgcttcta aaattgagtc tgcagcaaat ggtgctggaa cgtaaatgac tgaaaccgtt
     2341 gccccagttt catttttagc ttcttcaaca gtgttgaaaa caggaacgcc ttcaacaact
     2401 tgaccacctt taccaggcgt cacacctgct actattttcg taccataatc aagcatttgt
     2461 tttgtatgga aaagggcagt agaccctgta ataccttgta ccattacttt agtattctta
     2521 tctataaata cactcatctt agtgctccca tcctttcctt atgcttcttt gactagttta
     2581 acaatttttt gtgcaccttc agccattgtt gctgctggtt caattgctaa tcctgagtct
     2641 tttaagattt ttttacctaa ctcaacattt gtaccttcta ggcgtacaac tagtggtaaa
     2701 gttaaatcta cttcttttac agcttcaacg ataccttctg cgataacatc acatttcata
     2761 atgccaccga aaatgtttac aaaaatacct ttaacatttt catcacctaa aatgatttta
     2821 aatgcttcag ttactttttc tctagtagcg cttccgcctg catctaagaa attggctggg
     2881 tttccaccga aatgattaat cgtatccatt gttgccatgg ctaaacctgc accattaacc
     2941 atacatccga tgtcaccatc taatgcaatg tatgataaat catgtttaga cgcttcaatc
     3001 tctttcggat cttcttcttc taaatcacgt aattctacaa catctttatg tctgaataat
     3061 gcattatcat caaaattaat tttagcatct aatgccaata catcaccatc agctgttgta
     3121 actaatgggt tgatttctac gattgaacaa tctttttcaa tgaatacatt ataaagtgct
     3181 aataagaatt tagcagcttt gttaacagat tctttaggaa tattaatatt aaacgcaatt
     3241 cgtcttgctt ggaatggtga aagtccgatt actggatcga tagtttcttt gaagatcttt
     3301 tcaggagtct tcgcagcaac ttcttcaatc tcagtgcccc cttcttcaga cgccatcaat
     3361 gttacttggt cagtcgcacg atcaataacg aatccaacgt aatattcttt ttgaatagca
     3421 caaccttctt cgatatataa acgcttaatt tctttacctt ctggaccagt ttgatgtgtc
     3481 accaaagttt tccctaataa ttcttttgca tatgtttcta cctcagataa agatttagca
     3541 atttttactc cgcctgcttt acctctacct ccagcatgaa tttgtgcttt tacaacataa
     3601 acatcagaat ttaattcttt tgctttctcc accgcttctt cagcagtaaa tgctactcgt
     3661 ccttctggaa ctgcaacgcc cattgaacga aatatttctt taccttgata ctcgtggata
     3721 ttcatcttcc atcctcctgt tacttaggtt aagttccctt acaattataa aaaatgtaag
     3781 cgctattgta aacttaaatg ctactttttt atcatttaat tgaattttac gatttacagt
     3841 aacgatttta taggttcaaa gctttttcta tgctctttca taatgccaat atcatcgatt
     3901 gctagtaaat gttgtttggt accgtaaccc gcgttttttt caaaaccata ttcaggataa
     3961 tctttagata actgtgtcat ataatcatca cgaaaaacct ttgccatgat acttgcagct
     4021 gcaatggaca cacttcttgc atcacccttg attaaagata cttgaggcag tgcattatca
     4081 agcgtcatcg cgtctatcaa taaatgcgtt ggttgtactg ataatccatc aatagctcgc
     4141 tgcatggcga tttgagtagc tttataaata ttaaattcat ctatttcttc agctgtcgcg
     4201 atcccatatg caaaagcagt aacttcattt tttagtgctt catttaattc tagacgtttc
     4261 gtaacaggta cttttttcga gtcatcaagg cccaaataat tgtgatttga atttaaaatt
     4321 gttgcgcatg caacgactgg acctgctaaa ggtcctcttc caacttcatc aatcccacaa
     4381 ataatagcat taggatgctc ttttaatatt tcattttcaa agtaagtcat ttcaacatac
     4441 ttttctttta aagcttgttc tttttctaac gcttttctgc gcctagctat ggcattttga
     4501 acaccttttc gctcatctaa aaagcattca tgattttcta attcttctat tgtattaacc
     4561 gcattaatca actgcgtaac ttctttaatt gttagcgtca tttgctaatt cctcagtcat
     4621 atctttaaaa atatcaaaac aataatttcc tattttagca tttcgaatat cataaataat
     4681 cagttcaatg actgcttcgt aatcaatttc attaccacgt cgaattaagc cacgtttttt
     4741 ccctatcgca tcaaaccacg ctatgatttc tgcatcttca ggaacttcaa tattataatg
     4801 tgactttaat cgcgctaaat cattttgaat taaaaagttt aatccataga tggcaacttc
     4861 atctaagtgc acaatactat cttttatcgc accagttaaa ctcaacttct taccgacttc
     4921 ttcatcttca aatttaggcc aaagtatccc tggtgtgtct aatagttgta atgcattacc
     4981 aactttaatc cattgttgtt gtttggtcac acctggttta ttaccagtct gcgcaatact
     5041 acgctttgcc agtttattta ttaatgtgga tttaccaaca tttggaattc caacgatcat
     5101 tgctcttatc gctctaggtt taagtccttt cgctttttcg cgttcaaatt tttcagcagt
     5161 cgccttaatt gctgcagctt ccactttctt taaattttta ccgtgcttag catccactga
     5221 tacaggatag tatcctttat caataaaaaa ttgttcccat tttgacatct catttaaatt
     5281 agacatatct tttttattta atataacaac acgtggtttt tggttaataa cttcatctat
     5341 catagggttt cttgaactat atggaattct tgcatctact agttcaaaca ctacatctac
     5401 tttttttaat tgttcactta cttctctttt ggctttcgcc atatgtcctg gataccattg
     5461 aataaccatt caattcacct tactttcgtc aattcttttc tcattattaa aatctataac
     5521 ctgttaatta tagaacaaat tcattaattc aactatattt attttaacat tgtcacactt
     5581 cttaacaatg ctttagattt aggtataagt gattatttca agttttctac aattaaaaat
     5641 catcatttgt taaatctaag ttacttatta agtgacattc atctttatca gtatgacatt
     5701 tttattattc aatatatttg tttaatgata aactgaaaat gatccaaagt ttaagtaact
     5761 atgataaggt atctgttcca cagtcatctt cgtctttacc tatcaagaac ctcattaact
     5821 ttagtttcag ttaaacttaa ttttaaattt gaaagttgag gataatgttg tgttaaaaaa
     5881 gtggctaaat tcaaacgtca aacaattctt tgttataact ttcattagtg taatattaac
     5941 gcttatttta ttttctactc atatctatga ttatattgtg aatggtactg tttttagcgg
     6001 ggctggagat ggattccgtc aaatgatgcc atttcaaatg tatttgtatg aacatctacg
     6061 tagtttttct agtttatatg atgcatcgtt tggattaggt ggcgattata tgaaaggact
     6121 atcatattat tattcgctgt cacctttaat gtggctaaat tttctattca ttaaaatagg
     6181 agaaacggtt ggtatattta atccgacgac aatacatttt tggccgacaa accaacttat
     6241 tatggctatg atacgagcta tcataacatt tgtcgtgacc ttctacttat ttaaaatatt
     6301 acactttaaa cgctcagcaa atatgatcgc tacgatttta tacggcatgt caactgtcgt
     6361 tatatacttt aattttactt ggtcatttta tggaaattta ttatatttat tgccattatc
     6421 gattcttggt ttggaaagat attttcaaca acgcaaaatc ggtattttca ttgttgcgat
     6481 agccttaaca ctatttagca atttttattt cagttattat caagctatta ttataggttg
     6541 ctactattta tatcgactca ttttcactta caaatatgac attgtctcta gaacacaaaa
     6601 attaatttgc gtcatatctg ctacagtttt gagtgtgtta tcaagtgtat ttggtttatt
     6661 cactggcatt tctgcgtttt tggaaaatga cagaaagcaa aatcccaatg ttgatatacc
     6721 gtttttgaca ccacttgatt atcattattt tttctttagc gatggatttt atattacgat
     6781 ttcaattctt actatcgttg cattattgtc attcaaactg tatcgttttt acttttatag
     6841 acttttcgca atagtaacat ggatattatt tataggttca ttatcacagt atttcgacag
     6901 tgcttttaat ggtttttcat ttccagaaag gcgttgggtg tatatcttag cactatcatc
     6961 aagtgctctt tgcggattgt ttattcaaca tttatcaaca ttaaatatga aatattattt
     7021 aatcagaaca atacccgtat gcatcatcgc aatactttat gtattactat caccgacaca
     7081 cccacttgca cttatagtag gtattatcct gctaatagtg cttgccgtta ttttaaaatt
     7141 tagtttatgg cgttataaaa aattaaccgt tgcaatatta gtattaatcg ttatgattca
     7201 acaaatcgtc attttagata acaacaaaaa catggcaatc aaaccttatc aacaatcatt
     7261 atcaacgttg aaacaacatg attaccatag taactatgta aaccagctta taaaaaagat
     7321 aaatcaaaat gcaacaggct catttaatcg cattgattat atgtcagact atgcattaaa
     7381 ttcaccattt atatatcatt ataatggcat ttcattatat tctagtattt ttaatggaga
     7441 cattttaaaa tattatgaca agacactcca aattaatatg ccaatcgata aaaacagcac
     7501 ttatagatta cttggcaatc gtcaaaattt actatcactt tggaatgtta atgatcgaat
     7561 tagagtgaat catgatgaca acttaccata tggatttaaa attaagtctg aacacaaaga
     7621 caataaagtt cgttggattc attctaaaaa taccatccat tatccaagtg cacatattac
     7681 aaataaggtc ttttccaata aagaattaaa atctccatta gataaagaac aagcaatgtt
     7741 gcaagggatt gtttctaaca atattaaaga tgttaataca cattttaaag ccaataaaaa
     7801 tttactatca gattcaacaa ttaaattaaa tagtgcagcc tggcaatctc ctacaaaaca
     7861 tttattacaa gttaaacaaa ataatggtgg tctaactgta cagttgccaa aatcagtttc
     7921 taatcaattt aaagatttgt attttgaaat ggatttagaa ttactttcgc cggataaagc
     7981 tcatgatgtt aaagtgaatg aatatacaca agaaagaaat aaactcactt ataaatatcg
     8041 acgcgttgta acacccgtaa cgatacgcat taaagctcca gatagaatta gattatcatt
     8101 gcctaaaggt aagtatcgag taaatttaaa agggatatac ggcgaagatt ataccacgct
     8161 taaagacgct tcaaattcat tagaagctgt caaagttagt aagacaaagc atggttatac
     8221 tattactaaa aataaaaatt catctgggta tattgttttg ccaacagcat ataatcaagg
     8281 tatgaaagcg acatcaggtg atcaaagtct taaagttgaa caagtaaatg gtgttatgac
     8341 cggcattaaa gcacctaaaa atataacaaa gattcaattg agctataccc caccatacta
     8401 ttatttactt ataacaatta ctatatttgg cattatatgt agtattattt tcacgagatg
     8461 ggcaagacaa aaataatgtt gctacgcata tatttaataa atctactaag tccttttcaa
     8521 tggcacttag tagatttttg tttatataac cattttgagc aaacacgtta tgaatagaga
     8581 aggaataaac aattacatta tcaactttac attaactaaa cagcaaaaaa agccatctat
     8641 atcactccat aaaagtgacc tagatggctt tattgtttga gtcgaatttg actcatacat
     8701 agctttgttt aaatgctgat taacgaattt cttggattct agcagcttta ccacgtaaac
     8761 tacgtaagta atataattta gcacgacgta ctttaccacg acgtttaact tcgatttttt
     8821 caatttttgg tgtgtgtaat gggaatgtac gttccacgcc aacacctgat gaaattttac
     8881 gaactgtaaa cgtttcagaa acgccaccgc cacgacgttt aattacaacg ccttcgaata
     8941 cttggatacg ctcacgagta ccctcaatga tacgtacgtg tacacgtaaa gtatcaccag
     9001 gacggaaact tggtaagtct gtacgtaatt gtgatttagt tactgcttcg attaatttgt
     9061 gatttgtcat tataaattct cctcttcaac ctatgttctt gcctcgacaa atatatagca
     9121 gcggatcata gtgattttag tgtttccaca ctcagattat gttagcacaa tactaacctt
     9181 ttttcaatcc tattttatat ctttctaata tttgcttatc tgcattagtt aatggatatt
     9241 tttcaattaa gtcaggtctt ttattatatg tgcggatcaa cttttgctca tgtctccatg
     9301 catcaatatt ggcatgattt ccagacaata aaacatctgg aactgttaga cccttaaatt
     9361 cacgcggacg tgtatattgc ggaaactcta ataacccatc tgaaaatgaa tcgtcttgat
     9421 gtgactgttc attacctaaa acacctggaa tcagtctaac aatagcatca gtcatggtca
     9481 tcgctggcaa ttctccacca gttaaaacat agtcacccat tgatatttca tctgtgacaa
     9541 gatgtgttcg gatacgttca tcgtaacctt cataatgtcc gcatatgaaa acgatgtggt
     9601 cggccttgct taattcaaca gctttctgat gtgaaaatgg ctcgccttgt ggacacatta
     9661 aaataacgcg tgtttgttct gtgacatcta agtcttccat cgcattaaaa acaggttcag
     9721 gctttaacac cataccttgt ccgccaccat acggataatc atctacttgg ttgtgcttgt
     9781 taattgcata atctctaaaa ttaaccgtat tgatttgtaa tttattgttt tcttgggcac
     9841 gtttcataat tgaatgattt aaaacaccat caaacatttc aggaaataaa gttaaataat
     9901 caattttcat taatccaaca atccttccat tggcgtgatg ataatttttt tattttcaac
     9961 atccacttct tttacaacat cagcaatata aggaatcaaa tattctttag atcctttaat
    10021 cacccacaca tcattcgcac ctgtttcaaa tatattaatt acacgaccta ttggtgtttc
    10081 ttgatcatca aaaactgtac atcctataat atctgaataa taaaattcat tttcctcaag
    10141 tacgatatct tcatgatcac gctcttgata aatagaactc ccttttaaat gctcaatatc
    10201 attaatatta ttaatacctt caaatgtaag catatgaagc cctttatgca ctctatgtga
    10261 cttaacagta tattcaaggt cgttattgtt atgcacaact gtcagcactt gaccgggttg
    10321 aaaacgaacg tctgtaaagt ctgaattgga ttttacttta atttcacctt taataccatg
    10381 tgtgttaaca atttgaccaa cttcaactct cattctatta cctccaactc tatgctttga
    10441 acacttctat tttaattgaa aataattcaa agaaaaaggg caccattatg gtacccaata
    10501 cgttctatat tactaacatg tgatattccc aacgtatcat tgagtaataa ctgtcagtgt
    10561 aaacgagtta ttcttattgt tagaacaatt ttacgctaaa ttacttagct ttcttttgtt
    10621 cgtcaaattt tttcataata ccttcttttg ataagatatt gtgaactgta tcagttggtt
    10681 tcgcaccatc atttaaccat tttaaagcta acgcttcgtc aactttaatt tctggagcat
    10741 tagcgctcgt tgggttataa gtaccgattt gttcgatgat acgtccgtca cgtggagaac
    10801 gagcatctgc tactacgata cgatagaatg gatttctttt tgaacctaaa cgtgttaaac
    10861 gaattttaac tgccatttat aaaatctcct ttaagtcatg ttttaatttt tattttctac
    10921 aagaaataat attaacaggt gtatgaatgt ttgtaaagag ttttttcttt accaggaaaa
    10981 ttattgaacc tttttcttac aataataact atgtgattac taaattagac tgttaatagg
    11041 ttataaaaat tcatagtaaa tttcaccacc tacgtgttaa gttaatttat ctcgtaagga
    11101 catgaatggt accacttcta ccgcacacgt tcccccaata taacttatta atattagcaa
    11161 caataacctc atgttctagc aaatcagcaa tttcagtttt taattattat tgtgtatcta
    11221 aaaatatgca ccccataaag ttatattctt tagtataact ttcgggatgc acatctcatt
    11281 gcaacaaaaa tcagcgtgtt atattaaaca ttaaaacggt aaattcatac cttttaacat
    11341 attttgcatt tgattgcgtt tacctttttt acctttaccg ccaccagtga actgtttcat
    11401 cattttcttc atatcgttaa attgtttcat caaacgattg acttcttgta atgaacgacc
    11461 agacccttta gcaatacgct ttttacgtga tacattcaat gtgtctggat tgtttctttc
    11521 agccggcgtc attgactgga taatcgcttt aatatgatca atttgctttt cactcatatt
    11581 aagcttatct agccctttca ttttattcat acctggaatc attttcataa tatcatccag
    11641 tggtcctaga tttttcacct gatcaagttg ttctaaaaaa tcatctaaag taaacgatga
    11701 ctcacgcatc tttttctcta aatcttttgc tttttcttga tccacatctt gttgcgcttt
    11761 ttcaattaaa cttaacacat cacccatacc taaaatacgt gatgccatac gttcaggatg
    11821 gaatagctct aaaccatcta acttttcact cataccaaca aatttaattg gtttttgtgt
    11881 caccgaacga atagataaag ctgcaccacc acgtgtatca ccatctaatt tagttaaggt
    11941 aacacctgtg acatcaagtt gatcgtcaaa agattctgca acattgacag catcttgacc
    12001 cgtcattgaa tcgacaacta acataatttc gtttggttta gcaatgtctt ttacttcttt
    12061 taattcgttc atcaatgctt catcgatgtg taatcgacct gctgtatcaa tgattacaaa
    12121 gtctaaatgt tcttctttag catgttttaa tgcattagtt acaatttgtt gtggctttac
    12181 ttgatctcct tcactgtata caggaatatc aatttgtttc cctactgttt gtaattgatt
    12241 tatcgctgct ggacgataaa tatctgctgc aactaacata ggttttttgt tgtatttttt
    12301 acgcatcaat aatgctaatt tacctgcagt tgttgtttta ccagcacctt gtaaaccaac
    12361 catcataaca acagtaggtg gtttatttga catattaatc gacgtatttt ctccacccat
    12421 caactgcgtt aattcatctt gaactatttt aataacttgt tgccctggtg ttaatgattg
    12481 cattacatcg gaacctaatg cgcgttctga tactgtttta ataaattctt ttaccacttt
    12541 aaagtttacg tcagcctcaa gtaacgctaa tcttacttca cgcatcatta tctttatatc
    12601 agcttcagta agtttaccct taccacgcat tttttgcatc gtcgcttgca agcgttctga
    12661 taacccttca aatgccatat cgatgtcctc cctcaattta tatctattct aagtcttcta
    12721 attgttgaat ataacgttgt atttgttctg gattacttaa atgttgtttc atttcatcat
    12781 atatttctcg gcgttgctca aatttctggt ataattccaa tttcttttca taatcttcaa
    12841 ctaaatcgcc agttcttctt atattatcat aaactgcttg tctactcaca ttaaaagtat
    12901 ctgcgatttc acttaaagaa taatcttcaa gataaaataa ttccaaataa ttacgttgtt
    12961 tattcgtcaa taaggattga taaaaatcaa acaaataatt cattcgtaac gttttaacta
    13021 aatcattttg acccatgatt atcgtccttt tcttctgtta caatttgatc attttcaact
    13081 gttgttattt cttcattttg ttcaatcata tcagcgaata agccgtagac ataactttca
    13141 gggttaaatg gttgtaagtc atctaattgc tcacctaaac ctacatattt aactgggatg
    13201 tgcaattcat tacgaatggc taatacgata ccacctttgg ctgtaccatc taatttcgtt
    13261 aatacaatac ctgtaacatt tgttacttct ttaaagtttc tagcttgtga caacgcattc
    13321 tgaccagttg tagcatctaa acatagtaat gcttcatgag gcgcatctgg cactgctcga
    13381 ttaattacac gcttaacttt ttctaattct tgcattagat ttgttttatt ttgtaaacgt
    13441 ccagcggtat cacagattaa aatatcaaca cctttgtttt tagcggcatt aatcgcatca
    13501 tacataacag cagctggatc agaaccttca ctttggctaa ttacgtctac accaacacgt
    13561 tcgccccaaa ctttcaattg atcaatagca cccgctctaa aagtatcgcc cgcagctaac
    13621 attacttttt taccttccat tttatatcgg taagctaatt ttccaattgt tgttgtttta
    13681 ccaacaccat tcacaccaac cattaaaatg acatttaaac gaccatcttc taagttcata
    13741 gcttctgaat tatcatcttc ttgatggtaa atctctacga ttttttcaac aatgacttca
    13801 cgcaaatctt cagtatcttg aatattacgt cgttgtgctt ccatacgtaa ttcttcagtt
    13861 aacgtcatca ctgtattaaa accgacgtct gcagtgatta acatttcttc taaagcttca
    13921 aaaaagtctt catctacttt acgatatctc gctatcaaat tatttaattg ttcttggaaa
    13981 ttttgacgag atttttcgag tcctgcttta aatttagcac ccattttttg agcttcaatt
    14041 tcttcaaaat cttcaattga tattaagcca tcgtcatcaa aatccgcttc actcaacttg
    14101 cgtggcttct ttttcacttc agcattttct gctaaatcat ttgcgtcctg cgttgaacct
    14161 tcagaatgtg tatcttctaa tttgtcttga ccttgttctt ctgttaagga tttaacttct
    14221 tcattttctt tatttgttgc aaacttatct tttaagcgtt taaaaaagct cattattgct
    14281 cctccttcaa cacatcatct attgtattta aattcacact cacaagttta gtaacacctg
    14341 attcttgcat tgttacaccg tataacctat ctgcaaattc cattgttcct ttacggtgtg
    14401 taataacaat gaattgtgtt tcgtctgata actcatttaa atattttgcg tatctaataa
    14461 catttgcttc atctagtgca gcttcaacct catctaatat aacaaaaggt gcagatctta
    14521 cttttaaaat tgcaaatagt aaagcaatag cagttaatgc acgctcacca ccactcagta
    14581 acgataaatg ttgcaacttt ttacccggtg gttgtaccac aatatcaata ccagctgtta
    14641 aataatcggc ttcagttaat tgcaattctg catcgcctcc accaaacaat tgtttgaaca
    14701 cagctgtaaa atgtccttga atagcatgga aagtttcttt aaatctttca gtaacctctt
    14761 gatccatttc acttataatt tgctctaatg tttctttagc tttacgaaga tctgtacgtt
    14821 gttcacttaa aaatgtataa cgttcattta actcttcaaa ttgttcaatt gcatttaagt
    14881 ttacaggacc tagttcatca atcgacatct tcattaactt aactttttta cgtaatgcgt
    14941 caatcgattc atcactcgta tattcagatt tcgcacgttc aacagtcaat tgatattcat
    15001 catttaaatg atctatcgca tgatgaatta atacatctag ctttgattgt tcagctttaa
    15061 tatcttggta gtgattttcg atagctaaaa tatcttggtg acaaacttgt agtttagctt
    15121 cttgcgcatc gatttgttca ttcaactcaa tacgttgttg tttcaattta tctaattcat
    15181 ctgataagcg tgttctcgtt tcttgttgac cattaatttg atctttaata ttttgaaaag
    15241 cttgttcgcc catcacttca tccgaattaa agaatgcaat tttttcttta acacctttta
    15301 attgatgttt agtttgttga ttttgattat ttaatcgatc tattgtctgt tgttgtgttt
    15361 taatacgctc tttaaccaca gcaagatcag attgtttctg atgtaaggtt tgttgtgttt
    15421 tagtaacgct ttccttacct tctttagaaa gttttgtgta gcgttcaatt tcatcttcta
    15481 gtcgttttaa agatgcttta atactttcta gataagtttc tttttcactc aaagtttgtc
    15541 gacttttgtc actcgtataa ccatcatttt tttcaaattc gaattcttca tgatcatttt
    15601 ttatttgtgt ttcttgtgta gttaatctat cgagctccat ttcaaaatga tgcacttgct
    15661 ctttaagtgt attatgcttt tgactttttt caaaatacag ttcacttaat tgatcacttt
    15721 ttatcttcaa ctctttaaat tgttgttcaa atgattctgt ttgacgcaag taatcttcta
    15781 attggtgtct cattgttgtc aactcgtctt tttgagacag aatacttttt gacttacgag
    15841 cgccaccacc agtcatagaa ccaccaggat ttacaatatc accttccaaa gtaacaatac
    15901 gagttcgata tttaatcgca cgtgccaatt catttgcatg ctttaaatga tcaacgataa
    15961 tcgtattacc taataaattc ccgataatat tttgatattc tggtgctact ttaactgctt
    16021 ccgaagcgat actaataaat ccgtttgcct ctttagcaat agatttaata tcagtcgcta
    16081 ccactctact ctgtataaca tttaatggta aaaacgtcgc acgacctaaa ttacgttctt
    16141 ttaaaaattg aatagcctgg cgtccatctt tttctgaatc tacaatgaca tgttgtaatg
    16201 aagcacctaa tgctgtttca attgcctgag ttaatttaga tggcacatca ataatttccg
    16261 caactgcacc ataaataccc tttaattctt tatttttagc tttcaaaata tgtttgacgc
    16321 cattgaaaaa ataagtatat tcttcctctt gcgttgccaa actatcaata cgtgttttca
    16381 ttttttcggt atatcgatat gcttgataca atttctcttc gtattcattt tgtgcttttt
    16441 ttgtatcagt gaggtctttt tctatatttt taatttcttt atctacagca gaaagttctt
    16501 tgttggtctg ttgatattct ttttttgtcg tttttatttg accctgaata tctttcaatt
    16561 gctcaaaaac ttcaactaat cgagaatcta gtcttgattt tttagcctca ttctcttcta
    16621 tagtatgctt taaaaaacga atatcattgt taacatctga ttgctctgac attaatgtat
    16681 agtattcgtt tttaatttct tccaattttt catcatgtgc ttcgtctgaa acatatagtt
    16741 gttcttcaag ttcacgaatg acagcattga gttctttttg tttacttttc agagacttat
    16801 aagtatcttg agcttcagaa atctcatttg atatattttc taaaagctcc attaaatttt
    16861 cttgttcttc ttcatatcgt gcatttgttt cagattgatt tttcttacgt tcttccaaaa
    16921 catttaattg tcccgtatat ttttcaaagg cttccgtagc ttttactaat tgataattaa
    16981 gcgattcaac atcattatca agttgatgac gtttaccttt atattgttga atttgttggc
    17041 ttaaacgttg cttgtcagct tctttatttg cttgttggcc ttgtaaatca tttaaacgtt
    17101 gatctaattg tctattgtca tttgtatatt gatcaatatc gtgcactgta actacaatgt
    17161 cactatgttt catttgatgt gaaagtgtct tatattcttt agctatagct gcctcctctt
    17221 ttagaggttc tacgcgacct tccaaatcat ataaaatgtc ttctactctc gttaaattat
    17281 cttctgtttg gtcaagttta tttaatgatt cagccttacg ttttttatat ttaagtacac
    17341 cagccgattc ttcaataatt tgacgtctat caattggttt agcatttagt atttcatcaa
    17401 ctctaccttg cgagataatg ctatacgctt cttttcccaa tccagaatct aaaaataaat
    17461 cggcaatatc ttttaatctt gcacggtcat tatttatgta gtactcactt tcaccacttc
    17521 gatacaatct tcttgttaca ataacttcgt tttcatcaac actgagcttt ttagaatgat
    17581 tatctaatct taactgtact tcagcataat tttgagcttt gcgatgttct gcacctgaga
    17641 agataatatc ttccattttt gagccacgta atgatttagc cgattgttca cccaacaccc
    17701 atttaatagc atctgtaata ttacttttac cgcttccatt tggaccaaca attgcagtta
    17761 cacctttatc gaattgaaca ttggtttgat ctgcaaaaga cttaaatcca atggcatcta
    17821 ttgattttaa ataaaccatc ctaaactcct tatcacaatt ttaaagaaca catgtatacg
    17881 atattttagt atttgtatat taaatttcga agttgttcca taaaatatta aaaattcttc
    17941 cttaaatcct aaatcgaaca cctatatgtc gtatcatata aatttctatt taatttgttt
    18001 taattgctta taggcacttt cagcagcacg ttgttctgat tcttttttcg ttttcccttt
    18061 accttcagct attgcttccc cttgcagaat aacttctgaa gtgaatagac gatgatgtgc
    18121 cggtccctct tcttttatta aattataggt tacatcacct ttattttgct ggtgcacata
    18181 ttcttggaat tgtgttttaa aatctaccac gcctaataac tcattttgtt ctacatgtgg
    18241 gaaaatgact ttctcagcaa atttccaaac tatatctagt ccttgatcca aatacaatgc
    18301 cccaataaat gcttcgaatg catctgatat taatgatggt cttgtacgtc cccctgtttt
    18361 ctcttcacct ttaccaagta aaatcatttc gttcaatcca attttattcg caaatattac
    18421 aagtgagggc tcacatacaa tagtggcacg catttttgtt aaattccctt ctggcaagtt
    18481 gggatgttta tcaaataaat atcgtgaaac cgtcaattct aataccgcat cacccaaaaa
    18541 ctctaaacgc tcattatggt ctaaacgatt catattaaaa tcattaataa aactcgaatg
    18601 cgaaaatgct tgttggtata aatcaatatt ttgataagta aagcctaact ctgtcatttt
    18661 agtatcaaag cgctttctaa aacgattaac tatctcactt ttcttttgtt tagacattat
    18721 tttatctcct ttatgtgttg ctcttgaatc atctttaatt ttacgttaaa actattgata
    18781 attaaagaaa aaaactgagt cgacaatact gacgacccag atgtaagatt tattattttt
    18841 caagactgtt aataaattta acagcatcac caacagtgtt gattttttca gcttcttcat
    18901 caggaatttc agtaccaaac tcgtcttcta attccattac taattcagcg atatcaagtg
    18961 agtcagcgcc taaatcatct ttgaaagatg catcttcagt tactttatca gcgtctacac
    19021 ctaaacggtc aacgatgata tcttttactt tatcgaaatt ttccacgtcg attcacctcc
    19081 tttaaaaatg cctatacata gactactata ttttcccatt ttaaattcta aaaaacaagt
    19141 agaaatttta gaaacactta tcaatttatt gattagtgtt tcttagggag aaattctgga
    19201 accttatcta ggttgcttag aatttccccc gcaaagttga ctaggtcaga caattctcca
    19261 ttggatgacc agtcaaccac tgcgtcaatg agctttagct caaatatatt acatgtacat
    19321 tccaccattt acatggattg tttgacctgt aatatatttt gctttgtctg atgctaagaa
    19381 cgctactgta ttagcaatat ctgtgtcttg accaaaacgt gctaacggaa tttgagtcaa
    19441 catttgttct ttaagctcat cacttaaagc atctgtcata tcagaaacaa taaaaccagg
    19501 tgcaactgca tttacagtga taccacgaga tgctaattca cgcgccgcag atttagttaa
    19561 accaataaca cctgcttttg ttgcaacata gtttgcttgt cccggattac ctactgctcc
    19621 aacaacactt gataaattga tgatagcacc actacgttgt cttaacattt gtggtgttgc
    19681 tttttggata cagttaaata caccttttaa gtttgtgtca ataacatcat cccactcttg
    19741 ttctttcata cgcattaata aattatcgcg agtaatacct gcattattta ctaaaacatc
    19801 taaagaacca aattggctaa ctacttcttt aatcattgct ttaacttcat cagcatcggc
    19861 aacatttgct tgaatcgcaa aactgtcaac acctttagct ttgatttctt cgactactgc
    19921 ttcagctttc tctttgctgc ctgcatagtt tactgctaca ttatatcctt cttctgctaa
    19981 ttgtaacgca atactacgtc caattcctct tgatgcacct gttactaaag cactcttagt
    20041 cattttcatt ccatcctttc acatcttcta aagtttgaat tgatgttaac ttaacatctc
    20101 tatttatttt tttaattaag ccagataaaa cttttccagg accaatttca ataaaatgat
    20161 caacaccttg gtctattagc cattctgttg agttaatgaa ttgtactggt gaatataatt
    20221 gcttgaccat attagattta attacttctt tgtcagtttc accttgcgca tttacatttt
    20281 gaactacagg aaacttagca tcacgccatt caaattgatt aatgtaactt gaaaaatctt
    20341 cttcaatcac tttcattagc gatgaatgga atggtccaga tactgctaaa ggcatgacac
    20401 gttttgcacc taatgattta cctttttcta ctagctcatc aattaaagct ttgtgacctg
    20461 aaacaacaat ttgacctggg caattaatgt ttgctggttc aattattttg tcatcagatg
    20521 ataatgactt acaaatttca tcgactttat caaaatctaa tcccaatact gcagccatgc
    20581 ttcctacacc agtaggaaat gcttgcgcca ttaattgacc acgttttcta acaattttaa
    20641 ctgcatcttc aaatgataat acgtcagctg caactaaact tgaatattca cctaaactat
    20701 gccccatagt aaaatcagga ttcaaatttt ttagcgctgc taataatgcc gaactatgcg
    20761 tcaataaagc tggttgtgtg ttttcagttt cacccaattt accttcttca tcagtaaaca
    20821 ttgtctctaa aatatcaaag tctaatgtgt tcgctgctga agttaaaatt tcagttgctt
    20881 gatcattgtt gttaaacaaa tcttgcgcca taccaacttt ttgggcacct tgtcccggaa
    20941 aaataattgc tgttttactc atttgattca cctacagtct ctttcattgt ttgtacaata
    21001 ttttgttctc ctgcgatttt cgcttgtcta attgcagaat aaaaagcttt agcatttgaa
    21061 ctaccgtgtg ctttaactac agtaccttcc aatcctaata atacggaacc accgtattct
    21121 gagtaatcca tctttttagc gaattcagct aaatctttct tcaatattgc acctgctaat
    21181 ttatttttag tactactcat aatcgtatct tttaacattt taccgattga ttttgcagta
    21241 ccttctaaat ttttaaggac catgttccca gtatagccat cggtaactac aacatctgta
    21301 tcgccatcca ttaatgtctt cgcttcaata ttcccaacaa aattcaatga atgatcatga
    21361 tttaataact catatgattt tttcgttaaa ctattacctt tagctggctc ggttcctata
    21421 tttaataatg agattttcgg attatcaata cctctaattt tttgagcata aatatcccct
    21481 agttgcgcat actgtaataa gtgttcaggt ttagcatcag catttgcacc aacgtctaaa
    21541 aagacaaaac cttttccatc aatcgttggc aatgttacta ctaaagccgg tctagctaca
    21601 cctttaatac gtccaacaat gaataaacca gctgacatta aagcaccagt attacctgct
    21661 gacacacatc catctgcttc accagatttc acagcttcag ccatttttac cattgagcta
    21721 tcttttttac gtttaatcgc tctaacaggc tcatcttcca tttcaatctt ttcagaacaa
    21781 tgtctaaatt cgattcgttc atggttcaga ttatactttt tttcgtcacc gaaaagtata
    21841 atttctagat ctttaaagtc ttcaacagcc ttttgtacgg cttctaatac gatatcaggc
    21901 gcattgtcgc cacccatcat atcaattgct aatttaacca taattttatc ctcgcttatc
    21961 ataaaacatt ttaaaatttc ctttgaaaac taatgtatgt ttaacatatg actttacttc
    22021 gacgtaataa tgttttgcag tttgatttac aactcgtgct tctgctctta ccgtatcatt
    22081 taattttact ttttcaataa attgaatgct actctcatga gttaaaactg ttggttgctt
    22141 aattagcgca acacataacg aatttgcctg agcaaacagc acatgaccac gcgcaattcc
    22201 agttttatga aaaacagaat ccgatgtaat atctaaaatt gattgcgctt taacatttgg
    22261 attgacttga atcaaatcac caataaattc ttgttcttca atagaactta tttggtcata
    22321 attcttttca gcaactaatt taatacgctt ccttaattct ggtatgttta aataagtgcg
    22381 atctaaacga attgtttgta tactcacttg aaataagtcg cttagttcat ggtctgtgat
    22441 gaagggattg ctatcaattt gttgtctgat tgcttctcta cgtttatctt tctttagttt
    22501 caacgtctca cccctcattt tttagtacct agtcttaaac attcctcaca tatcataaca
    22561 ctttctattt aaccgactca acaaataact gtaatattta gctattattt agtcgaaaat
    22621 ttataaattg ttaaaatacc actttacatt taaatagttc aagtgtcata tcactcaatt
    22681 aacgaagtgt tgcactcaaa ttgacaaatc agcatggcaa ttagtcaaaa ctacgatgta
    22741 ataaattttc ttcaacaaaa tgacgtaaat gttgatacgt attttcaaag aatacgccag
    22801 attgaataag ttcagctgct tcatcacgag caacttctaa catacgataa tcttcaacta
    22861 aattggcaac taagaaatct ggcaatccac tttgtttaac accaaagaaa tctccaggac
    22921 cacgcatttc taagtctcgt tcactcaatt caaatccatc cgttgtttgt gtcataattg
    22981 tcattctttc aattcctgtt tctgttttag gggatgcaat taaaacacag taactttgct
    23041 ggtcacttct acctacacga ccgcgtaact gatgtaaagt tgataatcca aagcgatccg
    23101 catcataaat catcataaaa gttgcattcg gtacattaac acctacttca acaacagtag
    23161 tagaaactaa aacatttatc tcatgattac taaacttttg catgacctca tctttttcat
    23221 cggcagataa cttaccatgc aataacccta cacgggaaac accataatac tgttgtaaag
    23281 actcgtacaa tgcgacaaca ttttgaacat cttcgagatg ctcagaactt tctattagcg
    23341 ggcaaatgac atatgcttga cgaccttttt tcaactctga ggtcatttgc atcaaaactt
    23401 tatcgtattg ctcatgcttt gcccaagtag taatgatagg tttacgacct tttggtaatt
    23461 gtttaattga agacacatcc atctcaccaa aaactgatat tgctagtgtt cttggtatcg
    23521 gcgttgctgt cataaataac acattcgtca ttgcaccttt ttctcttaaa agctggcgtt
    23581 gattcacacc aaatcgatgt tgttcatctg taattactaa accaacatta tggaaaatca
    23641 catcatcttg aatcaaagca tgggttccaa ttaaacaatc aatcgtacca ttttcaagtt
    23701 gttctaaaag tatctttcgt ttcttacctt ttactgaccc agttaacaat gcaacgttca
    23761 tagaatctcc aaataaagcc attaaacttt cagcatgttg ctctgctaaa atttcagttg
    23821 gtaccatcaa tgctgattga taaccagcag tttttaacgc atacatacaa attgcagcaa
    23881 ctactgtttt tcctgaacct acatcacctt gaagtaatcg atgcatacgt attggtgctt
    23941 ttaaatctct aaaaatttca ttaacactgg atttctgtgc ttcagttagt tcaaaaggta
    24001 aacgatcaat aaatgattta acttggtcta tgtcataatc aatttcaatt gcttcgtcag
    24061 atgacttttc taatctattt agccattgca tacgtaattc gaataaaaac agttcagtaa
    24121 atgcataggt tctacgagca cgtaataaat cctctttact tttaggatga tgtaatgtgt
    24181 tcaaagtaaa gtccaaggtc tctaatttat atttttctct tagttcatca gttaaccatt
    24241 catgaattgt cacatcattt aacgcttgtc taatttggtc tcgtatttgc ttttgtttaa
    24301 taccttcctt aatacgatag actggttcta attgaacatc tgcgttttct tgagtttgtg
    24361 tcccttgtga attaaagaaa accctattac cagtaatttc ctgtttaacc ctattccact
    24421 tacctttaac agttatagtt tgatttaatt cgattttctt ttttaaatac ggttgattga
    24481 aaaaaataca tttgacagca atattattta ccattaaatg aacggttaat tttgatttat
    24541 ttcttccaaa aaatgcaact actggagctg tatatacttg tccttctatc gtaacgttag
    24601 attgatcttc tgcttgattc aaatcaatca ctgtattatc ttcatatcta gttggcaaat
    24661 aaagaacaag atcttccact gtatgaatat ttagttgttg caatacttct attttcttag
    24721 gacctatacc ttttaattgt aaaagagaat atggactttc tattaagttt actttagcca
    24781 aaataaatca cctatgtttt atttgatttt acgtatcatt tagtatgtta tttattttat
    24841 catgactaag ttgaacatga cattgttagc aactcgagaa cgatactttt aacccgcaat
    24901 atcactaaaa tagcaaaata aaaaactacc aactgatgat ttatcattgg tagtttttta
    24961 ttttaaattt ttattctact gaaaagaaat attgataaat tggttgtcca ccttcatgaa
    25021 cttccacttc tacatctgga tattgctctt cgatccagtt tatcatgtta tctgtaactg
    25081 cttgctctgc atcttgacca ataatcacag tcaatatttc actatcttct gctaacatct
    25141 catttaacaa ctcagtaacc gttgttaatt gatcactttg gctgcttaca atcttatctt
    25201 caatcaagcc cataaacgcg tcttttttaa tctcaacgcc atcaattttc gtatcacgaa
    25261 cagcgtacgt taatgaacca gatttaacgt tatttactga atcagccatt tgcgctttat
    25321 tttcttcaag tgttgcgtcc acatcatatt ggaatagtgc gcttatacct tgaggaatag
    25381 atttcgttgg aataacaaca gcttctgcat caacaatact cgctgcttgt tcacttgcca
    25441 ttaagatatt tttattattc ggtaaaataa ttgcacgttt acattttgat tgttcaatga
    25501 ctttaacgat atcttctgta gaaggattca tcgtttgtcc accactaatg atatgtgtgg
    25561 cacccattga tttaaatatc tctgaaatac cttcacccat agaaatagta ataattgctg
    25621 tttcaaccgt ttccattttc ggtttagctg tgtgctgttc ttttcgaatc acttcacggt
    25681 gctgttctct catattttca accttaagtt taattaattc accatattgt tgaccataat
    25741 taaacacttt acctgggtat tcggtatgca cgtgaacttt cacaatttct tcatcattaa
    25801 tgactaataa agaatcacca aattgactca tatcttgcct gaattcttgt tcatcaaagg
    25861 cttttttatt ctttccaaaa cgaaccatca tttcagtaca atagccataa ataatatctt
    25921 cagtattaat tacaccatgg aaatcatgtt catcatgtac aaattcatct ttatctatct
    25981 ttgcaacttt ggcttcaact ttttcacctt taagcgcttt taagaatcct tcgtaaacgc
    26041 ataacaaacc tttaccgcca ctatcaacaa caccaacttc tttaagtaca gctaataagt
    26101 ttggtgtgtt ttcaagtgat tcattggctt ttacaataat gtactccatt aattctatac
    26161 aatcttcagt attatttgct ttttctattg cagcttgcgc agcatctttt gcaactgtaa
    26221 gtattgtacc ttcaactggt ttcataacag ctttatatgc cgtttcaaca ccagcttgaa
    26281 aactttcagc taacaatttt gaattaattt cagattcact ttcaatattt ttacaaaatc
    26341 ctctgaataa ttgtgacaag atgacaccag agttacctct tgcacccatt agtaaacctt
    26401 tcgagaatgt tttacctaat tcgccgatat ttttcgacaa attattctct acttcttcgc
    26461 gacctgaagt catagtaaga ttcatatttg ttcctgtatc accatctggc actggataca
    26521 cattcaaaga atctaccaaa tctgcattgt tagataaatt ttgtgcccct tgtataatca
    26581 tatcggcaaa taatttacca ttaattttgc taatcatttc aagttgtcct cctaagcttt
    26641 cttgcctgta ttattcacac gtacaccttg tacatatata ttgattgaat ttactgatac
    26701 attaagtgat ttttccaaag tatatttcac tgttgattgt acattattgg caacttcaga
    26761 tattttcaca ccgtaactta caataatgta catatctata tccactacgc cattattttc
    26821 agttactttg atgcctttag cataattttc atgtcctagt atttccgcaa taccatctct
    26881 aacttgttgt ctagatgcca tacctacaat accataacat tcaacggcct ttccacctac
    26941 aaccgaagca atcacttcgt ttgaaatatc aattttaccg taatcatttg aaatctctaa
    27001 tgtcatatct ttgtccctcc taaatttcaa agtttattat tcgttttccc taatcacttt
    27061 taaaatttta ccacttagtg tagtcataaa cgctttaagt ttcattgatt atttttataa
    27121 aatatcaatg gtacaactta tcagaatgac ttacgcttaa atcacctatt atattttgag
    27181 taaactgaat ttacactcat gacgattatc tataggcgtt
//
LOCUS       DALAGF010000027        26345 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.027, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000027 DALAGF010000000
VERSION     DALAGF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26345
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.027"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            247..1599
                     /locus_tag="O2692_001073"
     CDS             247..1599
                     /locus_tag="O2692_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HCZ4298710.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKMMLDYLDTDDVTSLSTNDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(1661..2848)
                     /locus_tag="O2692_001074"
     CDS             complement(1661..2848)
                     /locus_tag="O2692_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4298711.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(3244..4020)
                     /gene="fetB"
                     /locus_tag="O2692_001075"
     CDS             complement(3244..4020)
                     /gene="fetB"
                     /locus_tag="O2692_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCZ4298712.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            complement(4013..4675)
                     /locus_tag="O2692_001076"
     CDS             complement(4013..4675)
                     /locus_tag="O2692_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ4298713.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLFSPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVNNKEKIENIIFKLADQGVAIMWITHSDDQSMRHFQKRITIVDGQISNTEELNQ
                     HE"
     gene            complement(4923..5999)
                     /locus_tag="O2692_001077"
     CDS             complement(4923..5999)
                     /locus_tag="O2692_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCZ4298714.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAIEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            complement(6354..6620)
                     /locus_tag="O2692_001078"
     CDS             complement(6354..6620)
                     /locus_tag="O2692_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCZ4298715.1"
                     /translation="MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            complement(6620..6877)
                     /locus_tag="O2692_001079"
     CDS             complement(6620..6877)
                     /locus_tag="O2692_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCZ4298716.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            7236..7691
                     /locus_tag="O2692_001080"
     CDS             7236..7691
                     /locus_tag="O2692_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCZ4298717.1"
                     /translation="MKKLCSLIVVALVCIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            complement(7859..9436)
                     /locus_tag="O2692_001081"
     CDS             complement(7859..9436)
                     /locus_tag="O2692_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HCZ4298718.1"
                     /translation="MTFLTVMQFIVNIIVVGFMLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            9631..10380
                     /locus_tag="O2692_001082"
     CDS             9631..10380
                     /locus_tag="O2692_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298719.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRLMHEDAFIPMIRSQIVASA
                     KYKWHILISFTLSVVLFLLLNHFNIFALNVDSFTVFDIYPLIKGYGSISLITIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSIILETLFYYPYINTWWEFIPALILAG
                     SATYLYLKSYRNFMVSYFYQLAVILVLHIFM"
     gene            complement(10604..11797)
                     /locus_tag="O2692_001083"
     CDS             complement(10604..11797)
                     /locus_tag="O2692_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4298720.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPR
                     PASSL"
     gene            complement(11809..12558)
                     /locus_tag="O2692_001084"
     CDS             complement(11809..12558)
                     /locus_tag="O2692_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298721.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKEVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            complement(12551..13366)
                     /locus_tag="O2692_001085"
     CDS             complement(12551..13366)
                     /locus_tag="O2692_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298722.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNPERLGVTGEIIFDGTSMLSLSESQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEQGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            complement(13363..14232)
                     /locus_tag="O2692_001086"
     CDS             complement(13363..14232)
                     /locus_tag="O2692_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4298723.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            complement(14229..15164)
                     /locus_tag="O2692_001087"
     CDS             complement(14229..15164)
                     /locus_tag="O2692_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4298724.1"
                     /translation="MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            complement(15177..16775)
                     /gene="cntA"
                     /locus_tag="O2692_001088"
     CDS             complement(15177..16775)
                     /gene="cntA"
                     /locus_tag="O2692_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HCZ4298725.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            complement(16918..18219)
                     /gene="cntM"
                     /locus_tag="O2692_001089"
     CDS             complement(16918..18219)
                     /gene="cntM"
                     /locus_tag="O2692_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HCZ4298726.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKAKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFNKDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMVILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQHLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            complement(18212..19030)
                     /gene="cntL"
                     /locus_tag="O2692_001090"
     CDS             complement(18212..19030)
                     /gene="cntL"
                     /locus_tag="O2692_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HCZ4298727.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            complement(19041..19862)
                     /gene="cntK"
                     /locus_tag="O2692_001091"
     CDS             complement(19041..19862)
                     /gene="cntK"
                     /locus_tag="O2692_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HCZ4298728.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            complement(20729..21094)
                     /locus_tag="O2692_001092"
     CDS             complement(20729..21094)
                     /locus_tag="O2692_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298729.1"
                     /translation="MLKEKESFRLLYQAIREIADKIGDNQLETNSVSLLLLDFDFEHE
                     VFDELYLVILKYLNTVSIENISHSELLNLIENTIPEDREINTFVKNKIIIGFANNYFP
                     ELQVLANEIKSDMASSLKQ"
     gene            complement(21357..22175)
                     /locus_tag="O2692_001093"
                     /pseudo
     CDS             complement(21357..22175)
                     /locus_tag="O2692_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503368.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            22408..23946
                     /locus_tag="O2692_001094"
     CDS             22408..23946
                     /locus_tag="O2692_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCZ4298730.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            complement(24058..24480)
                     /locus_tag="O2692_001095"
     CDS             complement(24058..24480)
                     /locus_tag="O2692_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCZ4298731.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDRL"
     gene            complement(24932..25351)
                     /locus_tag="O2692_001096"
     CDS             complement(24932..25351)
                     /locus_tag="O2692_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCZ4298732.1"
                     /translation="MSKKHVFIIIGVILCICTVSTVMHFKMKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKETNTIKSVHFTNLETSPMGSAVIEGYINENKEDDFTAYASPEHNYQ
                     FGGAMIKSEGVDKLLKPAHERKSPEKIKEELDKKEGH"
     gene            complement(25369..25788)
                     /locus_tag="O2692_001097"
     CDS             complement(25369..25788)
                     /locus_tag="O2692_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCZ4298733.1"
                     /translation="MSKKKILILTSIMLIILISVAGIYLKVKYDEKEKQKAIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTNSETSPMGSAVIEGYINENKKDNFTAYATPEHNYQ
                     FGGAMIESEKLSELLKPANQLKSPDDIKKELNKKKSH"
     gene            complement(26055..>26345)
                     /locus_tag="O2692_001098"
     CDS             complement(26055..>26345)
                     /locus_tag="O2692_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCZ4298734.1"
                     /translation="ERITLYLKHNTKEPNTIKTVHFTSLKRGPMGDAVIEGYINENKE
                     DDFVAYGSPEHNYQFGGSLIKSKNLSTLLKPVHQTKSPDEIKKELESKKNDR"
BASE COUNT         8395 a         5032 c         3855 g         9063 t
ORIGIN      
        1 tatttttaag tacacattag ctgtggctaa tgtataagaa ccactacata ataaatcatt
       61 tgtggctctt tatcatttct gtcccactcc cgtagaagta catcatataa tgctgaaaat
      121 ggtttgagtt aaaacagata tcaagctcgt ctgattcagt cacaaaattg tcttgttata
      181 cttgtcacct atcatctata gaccgtggta tgattaaatt ggggatgata aaggaggtta
      241 ataaatatga agattaatac tacaggtggt caaattcatg gtattacaca agatggttta
      301 gatatcttct taggcattcc ttatgcagaa ccaccagttc atgacaatcg ctttaaacat
      361 tctacgttaa aaacacaatg gtcagagcca attgatgcaa ctgaaataca acccatccca
      421 ccgcaaccag acaacaaatt agaagatttt ttctcctcac aatctacaac ttttactgaa
      481 catgaagact gtttatatct aaatatttgg aaacaacata atgatcagac gaagaaacct
      541 gtcatcattt atttttatgg tggtagtttt gaaaatggtc atggtacagc cgaactctat
      601 caaccggcac atttagtaca aaataacgac attatcgtta ttacatgcaa ttatcgttta
      661 ggcgcattag gatatttaga ctggtcatat tttaataaag attttcattc caataatggc
      721 ctttcagatc aaatcaatgt cataaaatgg gtgcatcaat ttattgaatc cttcggtggc
      781 gacgctaata acattacttt aatgggtcag tctgcaggca gtatgagcat tttgacttta
      841 cttaaaatac ctgacattga gccatacttc cataaagtcg ttctactaag tggcgcacta
      901 cgattagaca cccttgagag tgcacgcaat aaagcacaac atttccaaaa aatgatgctc
      961 gattatttag atacagatga tgttacatca ttatcgacaa atgatattct tatgctgatg
     1021 gcgaagctaa aacaatctcg aggaccttct aaagggcttg atttaatata tgcgcctatt
     1081 aaaacagatt atatacaaaa taattatcca acaacgaaac caatttttgc atgttataca
     1141 aaagatgaag gcgatattta tattactagt gaacagaaaa aattatcgcc gcaacgcttt
     1201 atcgacatta tggaattaaa tgatattcct ttaaaatacg aagatgttca gacggcgaag
     1261 caacaatctt tagcgattac acattgttat ttcaaacagc cgatgaagca atttttacaa
     1321 caactcaata tacaagattc aaacgcacaa ctatggcttg ctgaatttgc atggcatgat
     1381 acttctagcg cacactatcg cagtgcttac catattctag atatggtatt ttggttcgga
     1441 aacttacaaa tattagcagc acatcaatat ccaacaactg ctcaccttaa atttttaagt
     1501 cgacaaatgc aaaatgattt agctaatttt gctaaaagcg gtaaaatgcc ttggccgatg
     1561 tatcataatg aacgtcgcta ttatcgcaca tatcaataac gttatcgcat ctaaaaaagc
     1621 acacctgaat ttgatagcag gcgtgctttt catatgagaa ttatgatgat ttaaatgttt
     1681 tagcataatg attatttttc aatgtgaaaa caacgattaa tcctaatgcg ccaatgatgg
     1741 cactcgtata tgtgatagct tgaacagaaa catgtgtcat gaccaagcct ccaataatgc
     1801 ctccaacacc aataccagcg tttaaactag acatgttcca actcattact tggcttgtgt
     1861 cgccttcaac atgttgaatc acaccgcttt gcactgctgg attagtactc cattgcatga
     1921 tattccaaat aaatagtcct gctaacaata gacctgaacc aggtaagatt aaattcataa
     1981 gtaacatcat gacgataaaa atagaaacag aaatcattaa ccaacgctta cttgtaattt
     2041 tatcggagaa tataccacct aatgatgttc caataacgcc agcgattcca tttactagaa
     2101 gtgctaatga aacgaatgac atatcatgac cattagataa aataagtgga tttataaaga
     2161 cgaatgtcac tgagtttgca atcaatacta aaaacgtaat aattaaatat tttgctactt
     2221 cagcaggtct taatattttc gaagtaacat gattttcatg agatggtgtc tcatgattca
     2281 cagggcctcg ttgtatttcc tgatccttcg gtaaatagat catcatcaag aagccaacaa
     2341 taatactcac aataattaag aatagaaatg tatagcgcca acctactaaa tcgccgataa
     2401 ccgttccaat tggtacacca aaaacattag caccactaaa ccctgtatag acaagtccaa
     2461 tcattttacc acgattttta ggtgctgata acatcgctgt aatagctaat acttttacga
     2521 taattagtgc tgctgctgca gatgagataa ttctaccaac tactaatatt gaaaaatttg
     2581 gcgctacagc aataatgcca ttaccaatga taaatataag taatgtccat aataatacag
     2641 gccttgatga aaaacggttc gttaatttaa ccagaatagg tccacatatc gcaaatgtta
     2701 aagcgtacat tgtcactaat tgaccaacga cagcttctga tacatgtaag tcctgactca
     2761 tcaagttcat aattcctgca accatcattt caaccattcc tacgataaaa atacttaaaa
     2821 tgaatgtaaa tagtcgcatg attgacaatt taaaactcct ttcaaacaaa atttccgata
     2881 ttcatttccc ttagaataac gatttattaa aaaatgtgtg caacacaatt ttatatataa
     2941 ccattctatt atataatatt ttgctttaat tttctgtgct taattggaaa attattgaaa
     3001 ttgtttttca gtatttgaaa aattaacaca agtatgtcat ttaataatta tgtataatta
     3061 cttcagtttc aacaataact ccaaaaaagc acgcacgctt tatatgtcag aacagataac
     3121 tgactacaaa gtatgcgtgc ctttatgaat attttcgctg cgctaatgaa tagcgatact
     3181 atatgtgcgc gtcacttcat ttccaattaa aactaatgcc taaatctgat gcagtaaaat
     3241 ctatcatgat tcactcttaa catcagtatt tcgtgctact aattgatgtc ttgcattgaa
     3301 aaattgacca tagcttaaat atgtcgcaat caaagcagac ataatggtcg cagttgtatg
     3361 aataaacacg actaacaatt gaaatttaat cgcttgtaaa ggtggtacgc caccaataat
     3421 taagcctgtc atcataccag gaatcgacac aagcccatat gttttaaccg aatcaatagt
     3481 tggcactata gctaaacgaa tactttcacg tattgcacct ttagaagcca atttaggtgt
     3541 agctgcaagt gataatttag attcaatatt agtaccatct tgtacgaatg cacgatctaa
     3601 attctggtaa gctaaattaa ttgcaatcaa gccattattt gcaagcatgc cgccgatagg
     3661 tataacttca tttgcggtaa attgaatggc ccctgtcgca atagtacctg caagcggtaa
     3721 tgccgttcca atgaagatag ctagaaatga tatccaaaac acatgatgca tcactggtga
     3781 tgctcgacta attgtattcc atgatgcatt aataataatg accaatacac ataaaataag
     3841 cagccattta tcgtttattt taaaaatata atgcagcaaa aatcccaaaa tgattaattg
     3901 cacaactgct cgtaatgtcg caacaattaa atctttaata atatgtaaac cttctttata
     3961 tgaaatgata atcggaatga ctaaaagcaa tgccgtcaat ccaagtgccg tattactcat
     4021 gttgattcaa ctcctctgta ttagaaattt gaccatcaac aattgttata cgcttttgaa
     4081 agtgtcgcat actttggtca tcgctgtggg taatccacat aattgccacg ccttgatctg
     4141 ctaatttaaa aatgatattt tctatctttt ctttattatt aacgtctaat gcactggtcg
     4201 attcatctaa taaaagaata tccggtgtat acatcagttg gcgcgctata gcaattcttt
     4261 gccgctcacc acccgacata ttttccactt ccgaacttaa ttgataatgt cccaaaccga
     4321 catctttaat taattgcttt gcacgttttc tatcaaattt atcattacgt gcaagtgatg
     4381 ggaatatcat gttatcttca atcgtttcac caaacaagtc actttgctgc atcaaataac
     4441 tgattcgttg acgcaattct tccgggtcat aatcattata gggtttacct ttaaaataaa
     4501 gttctccact agttggacta aacaaattac atatttgctt ttgaaatgta cttttaccac
     4561 tacctgatgg acctataatg gcaatactct cgcctttatc tacttttaga ctgatatgat
     4621 ctagtatgat tctatcgctc gctttataca ctaaatcttt aatttctaac aacatttgca
     4681 ccgctcctca tgtaatatat caggtatatt attcccgttt ttttggtttt tagtgcaatt
     4741 acatttaaga aaaaatcatt ttcttgtaat atcaaaattt aggcataatt gattttcagc
     4801 atttaaatta aaaacaaaaa atcctattaa catggatact gtctaatgac taagaattaa
     4861 aattagacaa ttgtttaaca acctaccata ttaaaaggat tctttattat ttatggattt
     4921 gattaccaca tgatattttt ataactttca tcattcaatg aacggacaat ttcagtaaca
     4981 agacggatag aattttcata atcatctaca ttgagcactg aaacattaga atgcatgtat
     5041 cgcagcgtaa caccgattgt catcgttgga ataccttcat ttgcgacatg aatacttccc
     5101 gcatctgtac ctccacctgg tgtcgtatcc cattgtactt cgatgttatg ttccttagct
     5161 acatctttaa tatgctttcg caaaccttgg tgagcaatac ttgtagcatc catcataatg
     5221 acaactggac caccgcctag tttactatcg ctcgtttgac ctgacatacc tggggtatca
     5281 taagcaatac ctacatcgac agctatcgcc aagtctggtt taatcgtatt cgctgccact
     5341 ttcgcaccac gcaaaccaac ttcttcttgc actgtggcac cactgtataa gttaatgcca
     5401 atattttcat cttttaaacg ttttaatacc tcaatagcta atgcacagcc atagcgatta
     5461 tcaaatgctt tcgcagttaa atatttatca tttgcaagca cttcaaattc actatatggc
     5521 gtaaccatat tgcctacttc aacgccagct tcttcagctt cttccttgct actaacacca
     5581 atatctataa acatattttt gatttccatt ggctttttac gttcttcagg cgttaagaca
     5641 tgtggcggtt tagaaccgat gatacctcta atttctttgc ccgaatctgt tgtaatcgtt
     5701 actttttgag atagcatgac ttgattccac catccaccaa ctggcgtaaa tgaaataaaa
     5761 ccatgtttat caatctttgt taccataaaa ccaacttcat ccatatgacc agaaatcata
     5821 attgagtatt gaccattctc agcatttttc tttccaaaaa tgccacccaa gttatcttca
     5881 ataatttgat cactgacagg ctctatatag ttacgcattg cttctttaac ttgcatttca
     5941 taaccagcaa tgccgtttac atctgttaaa tgtttaagta atttgactga ctgattcatg
     6001 ttattcatcc tcctgtattt caacttaaaa ctattgtatc actaactttc agaaaaacgt
     6061 tccctaccgc tttatcattt tcaggcttaa atatttttaa aattttgtga ggaatccgct
     6121 taaaaacctc atatttctaa aatttaatat tccgaaaaag tcacaattta aaattcctta
     6181 ttataatacg ttattctttt attgttacta atttcaatct gtttagtctt gcgctattat
     6241 aaaattaaaa agtgtagaga taacaacgca ttaacgtcgc tattttccct acacttaaat
     6301 actagtttct ttaattaact tatcattaaa gttattcaga tattgaatat tcattaatca
     6361 taatgtgacc atgccgatag tattaatact tctttatttc gatcatctac ggtatagacg
     6421 accctgtgct gatggttaat tcttcttgaa tatcgctcta aatatttagg ctctaatttt
     6481 tcaaaagatt gtgttatttt atacggatca ttttttaaag tctcaacaat ttctaaaaat
     6541 gacttcttta aataagaatg ttttattttc tttaaatctg atttcgctga atttttaatc
     6601 ttaaccgtgt aattgctcat taaagattat cccaatcaat atcatctata tttgttgtac
     6661 cactattatc tttttctctt tctcttactt tgtccattgt accagtagat tcaagatata
     6721 ttgtctcttg tatacttttc caatcttcta aacctataat cacagcatta ttttcggcat
     6781 tattgccact aatataaatt ggttcgtgat tattattaac attttttagt aattgataaa
     6841 aatcttttct cgcttctgta gggctagtga taatcatata aaccctcctt tgttttctgt
     6901 tacgctaatt atagcgtacg cacttgagta cgtcaatatt accaacgagt tcatcgtatt
     6961 cttaatacct atatgctatc gttcacaaac ttttgttctt ttcttccaac ataccgccta
     7021 taatgctcaa aaagaaatca gcagtggaac acactacgtt ccctaccgct ttatcatttt
     7081 caggcttaaa tatttttaaa attttgtgag gaatccgctt aaaaaaactc atatcttcaa
     7141 aaaaaatgac aaaatttaaa tttttgaata acatcatttt ttagtaaatg ttataatttc
     7201 tttataatac aaatatcgaa agaaggcgac tgactttgaa aaaattatgt tcattaattg
     7261 tagtagcatt agtttgtatc attgcattat cagcttgtgg taaagaacaa accaaaacat
     7321 atgaaggtga tgttagtggt aaacatgtac taacttctat cacatataaa gatgataaag
     7381 ttttaaaaca gtctacaatc aacacaatta aatatgacga tctaggtatg gataaagacg
     7441 aagccaaaaa gttattcgcc aaatctgaaa gtattttcaa agaccttaaa ggcgtaaaat
     7501 acaaagtaga ctataaagat aaaaaagcaa ttgaacactt agacatagat tacacagaag
     7561 ttgacatgaa aaaattaaat aaacgtcttg gtgtttcgac taaagaaaat aaagatatta
     7621 gttttgaaaa acttgaaaag caattaaagc acagaggttt aaaagaaaaa gataaaatgg
     7681 acgacaaata gtttataact taaaaatgcc ctcagataag actaaggtta caaaccttaa
     7741 ttcatattct gagggcttta atatttgaag ttcttgtgtg accagcatcc actactaata
     7801 taaaattatt tgcagtaacg ctaaaatccg ctgctttcaa tttcccgaaa taattaagtt
     7861 aactaatgag ttttaattta taatcatgta tcgtttgtaa ctcaccatcg acttttcgat
     7921 atacaatatg atcagcagta atttctgtag gactggatac gccaacagct gctgcaatat
     7981 tgaataagcc ttcatgcaaa cttgttacat agtttgtgac acgatattgc ttttctccaa
     8041 caatcaatgc tttttctttc ttcgcatctg tcgttgcaac acctacagga cacgtattca
     8101 tgtgacattg ttgactcatt atacaaccga cactaatcat catcccacgt gcgatattta
     8161 caaaatctgc acctaaacct agtgcaatcg caattttatc tggtgtcact aacttaccag
     8221 atgccgccaa tttcacttta tctcgaatac catatttttc taacatgcca gacacaatag
     8281 gtagagctgt aaatagcggt aagccaacac catcttgtaa ttcttggaat gttgcaccag
     8341 taccaccttc accaccatca atcgtaataa agcttggata cttatctagt tccaccatcg
     8401 tacgtacaag tgtttcaatt tctgaaactt tgcttactac aattttgaat cctactggtt
     8461 tttgacctaa ttgctgcaac tgatcgacga aacgaatcaa atcttcagca ttatgaataa
     8521 attcgtaacg gttaggtgaa ttgattgttt tataaggttc aacatttcgg attttagcaa
     8581 tttcttcgtt taccttttca gcttccatat gaccaccacg agtcttagca ccttgtgcca
     8641 acttcagctc aaatgcgcgt acgttagata actgtgcaac ctctttaaat aaaccttcac
     8701 taaaattacc ttctttatca cgaacaccaa ataaaccggg accaatttgg aaaatgatat
     8761 ccccattacc ttttaaatga tattctgata agccaccttc acctgtattc atccaagtgc
     8821 ccgctttagc tagaccttta gataaagctg taatggcatt ttttcctaaa gcgccataac
     8881 tcataccaga ttgtcctacg atacgtttta aaataaatgg atgttttaaa tgttcaccta
     8941 attttattgc atggtcatca cttaagtaat acggatcaat ctttgtcggc acacgatatt
     9001 cttcacgact aaataaacgc tcattcgcga ttttataaat gaatgttgat aacaatgttg
     9061 tattatctac tgaaatctca ttacgttgca tcggaaacat tgtgttctgt atgtaaaagc
     9121 cgtcttgata atctttagta gtaccgaagc tggtcatacg agagttatat tttccagcca
     9181 aaacgatatt tttataatca ttacgtgaaa aaggtttccc ttcattatcc ccagaaaata
     9241 aatactgacg taattccggt cccatttttt ctgaaatata tctaatacgt gctagtaaag
     9301 gataattcct taatacacta tgttgtgatt gtcttttatc tttaattaac caaataagcc
     9361 cgataacaat aaccgtaagc atgaatccta caacgataat gttaactata aattgcatga
     9421 ctgtaagaaa cgtcattaca atacctcccc ccaaaatttc aattcaatat ttatgataca
     9481 ccttacaaaa caaaacacaa tggaagcgct tcattttata aaacaatttt atgatatgtt
     9541 tttcatttta aattttaatg tataaaacat acaatacaaa gtaatatgtg ctaaagtatc
     9601 tatataatac aactatttaa gaggtatact atgtcaaata caaataaaca ttacatagaa
     9661 gaagaatacg ctaccgaaca atcgcgtttt ttcaaacgtg atattggatt tattttcctt
     9721 tacatatttt tggttaacat cttgccgatc attatcgtct ttttaacttt tgggttagta
     9781 gctgtattca ctcaacaagc tccgtggggt ttctcttttc ctatttcaat gaacattagc
     9841 tatttactcg cagttattat ttcactagct atttttcgtc tgatgcatga agatgcgttt
     9901 attccaatga tacgttcgca aatcgttgct agtgctaaat ataaatggca tattcttata
     9961 tcatttacac taagtgtcgt actcttttta cttttaaatc atttcaatat tttcgcacta
    10021 aatgtagatt cctttactgt ctttgacatt tacccgctca ttaaagggta tggttccatc
    10081 agtttaataa ctatattttc gttaaccatt atattaaata ctttgatttg tcaacttatt
    10141 ttccgtcata ttttaattca agaattaagc agattaatac ctttaaagat tacaatagta
    10201 ttgtccatta tccttgaaac tctgttctac tatccataca ttaatacatg gtgggaattc
    10261 attccagcat tgatactagc aggtagtgcg acttacttat atttaaaatc ataccgtaat
    10321 tttatggtga gctatttcta tcaattagca gttatactcg ttttgcatat tttcatgtaa
    10381 aatttttcag agtattatcc attatccata cacatcaaaa agcacactta aaagtcagta
    10441 tcaaaactca cttttaagcg tgctttattt agatatactg tcgttctaaa aacgagccat
    10501 atagaatgtt tcatgtccta gcatgaagca catgaatcgc catgctcaat atacctttta
    10561 aaatttaaat attcattaac ttaatatgct ttaattcact tcattaaaga ctactcgctg
    10621 gacgtggtgt tggctttttc aagaaactca taatcattat tacaatgact aacataccac
    10681 aaattagata catccattta aatcctacta aatcggaaat aggtcccatc aatgcgccac
    10741 ctaacgatat acctaagtct gcacaggcaa taaataaacc taacaacata ttacgaccta
    10801 cttttggtaa gacgaagctt aagtatgatg ttaatgttgg gtacaccatt gcttgcgtca
    10861 ttcctattaa tatcgcacta ccatagaaaa taattgcacc tacttgcgga ccaaatgcca
    10921 ctacaaatga cgcgattact aataatgata gtacagatac catatattta ggatgccaca
    10981 taccatctga cggaatgtat ttccttaagt aaaatcttgc cgcaacaact gcgatggcct
    11041 gtattgtcaa aaagattccc gcattcgcga atcctaaact cactgtgtat aacggtacaa
    11101 atgtactaac tgcaccaaat acaatcgatg caacaatcat gataatacca ctgttcaaca
    11161 actctttatt tttgaaaaat tgcgcaaaaa cagttacagc gttaaacggc attttttcaa
    11221 tcttatctga cgtatcgggt tcctgttcag caaaggtcac gcgatagcca aagaatgttg
    11281 ttgttaatgc gataaagata atgacaattg caaatagtga aatattattt gcattccaaa
    11341 tacctacggc aactaatggt ccgattaagt ttggaatcgt tgaaaatagc gagtacaatg
    11401 atacaccttc agaacgatgt tcctctggta atgcatcaat aatacctagc tgtaaagaca
    11461 tagaaaagaa tgccgtacac acaccttgca taacacgtgc tacgaaataa ccttctaagc
    11521 cagtaaaacc ataaataatt aaagctatgg cattgataat caaaataatt cttaatactt
    11581 taatgggacc gacacgagca ataatttgtc ctgcccatgg tcgaaatacc attgctgtta
    11641 acatgtatgc ccccataacg ataccgataa ccgtattcgt tgcgcctaaa tcatgccctc
    11701 gtaaaggtat aaacacgttt aagattgcat tggcactaaa gaacatcaat gttaatatat
    11761 ataatctcaa aaagggccaa gccattgcac ctttcattat ctcactcctt agaatgatag
    11821 ttgtttttct attaattctc ttgtataagc attgtcactt ttatgcaatg cgcttgtcgg
    11881 aatttgttct tctatttttc cgtttttaaa aataattaat tgatcacata aatacgtggc
    11941 agcttgaata tcatgtgtga taaaaatata actcaactga cgcgtttcac gtaaatgaat
    12001 caataaatct aatatttgtg tttgaattga catgtcgagt gaactaatgg cttcatcaaa
    12061 caaaatatat ttagggttaa tacatattgc acgcgcaatc gcaacacgtt gcgcctctcc
    12121 acctgataac atattaggat atttatccat gtatgcctta gatagaccga cttcttccaa
    12181 caatgtaatt gcttggactt ccataacttc tttaggttgt ccatcacatt gacacatcac
    12241 ttcaaataag atttctctaa cagtctgaaa tggatgtaat gatgacgtat aatcttgaaa
    12301 tacagcacca atttgatgac gtctcacttt cttcttatgc atcggttgat catttaaggt
    12361 tacacaacct ttatccggtt tctcaatacc taatatcata cgactcaacg tcgatttacc
    12421 gctaccactt tctccgataa tcgcaatcgt cgcaccgatt ggacactcaa atgacacacc
    12481 tttcacgata ggtgttcgac gacgcttaaa aacatgtgcg ctttgatatg acttttcaac
    12541 atctttaatt ttaatcatgt acatcacccc tcatcacatg tttaaaatga tcattaatct
    12601 tcttcttcgt tgataataaa tacttcgtat aaacatgttc tggatgatgc aagactgatt
    12661 cacgtgtccc ttgttcaata agctgaccat ttttcatcac aacaacacgg tctgcaatct
    12721 tgttaataac cgttaaatca tgtgaaatga aaatcatcgc acagtcaaag tgttttttaa
    12781 tatctataaa tgcttccagt acatcatatt gtgtaattgt atctaaagcc gttgtcggct
    12841 catcagcaat gattaacttt ggtttcaaag ctaacgctaa agcaatcatc aatcgctgta
    12901 acattcctcc tgataacatg taagggtatg attttaatat acgtttagga tctttcaaac
    12961 ttaaataatc catatattca atcaatgtct tttcaatttc ttgtgtagac attgacgtat
    13021 gtactttcat agtctcaaac atttgtttac cgacagtagt tgatgggtca aaggcacgac
    13081 taccttgttg catgaccatc gcaatgtctt taccacggta ctttttcaat tgcgattcag
    13141 ataatgacaa cattgatgta ccatcaaaga taatttcacc tgtcaccccg agtcgttcgg
    13201 gattcaaacc aataatcgat ttacaagtga ttgatttacc actaccactt tctccaataa
    13261 cgcctaaagt ttcaccctta gttaatgtaa aattcacatc actcacgagt ggttgatctg
    13321 tccaggtatc tgtaatcgtc aaatgtttaa ctgttaacaa tgtcatgatt gcaccactcc
    13381 ttttttcaca gaacgaagtt tatctttaga agagatgcgg ggatcaatag caatttgtaa
    13441 agcatcggat aagaagttaa atgccatcac tataatcact atggcaatac ctggcgcaaa
    13501 catcatttca ggatgtgtaa acatcacttt tctagcttcg ttaagcatca tgccccactc
    13561 tgcagtaggc gctttgacac ctaatcctaa aaatgaaaag ccagatattt gcaagatcat
    13621 tgaacacatc gagctactag agatgatagc aatatctgct aatgttaatg gcataatatg
    13681 tttgtgaata attttcatat cattcatacc gattgtttta gcaaatctta catggtcaga
    13741 agcagtgtac tgcataacac ttgtacgtat aacacgacag aaccatgccc aacgcgtcaa
    13801 aataaatgcc atgataatat tttcggcacc cattccaaac aatgcaatta atgctaacgt
    13861 tacaacataa cttgggaatg ccaacataac atcacacgca cgcatgatta aggcgtcaac
    13921 aaacccttgg aaatatcctg ataagaatcc taaaatagat ccaataagta cagaaacaaa
    13981 tagcgcaaca aagacatata acaaacttgg tctaatcgca taaattaacc tagttaaaat
    14041 atctctacct aaatggtcag tacctagtag atgttgaaaa ctcatgccag caaatttgtt
    14101 tgctgtatcg atatggttag gatcataaaa tgtcacaagt ggtgctgcta atcctaaaaa
    14161 gacatataat acaataatgc ctaaagcaat tactgcacct ttatcttgta ataatcgttt
    14221 taaaattatc atcgtgcgcc ctcccttaat cttggattta atagcgcatt aatgatatct
    14281 gctaatgtat taaatacaat aaataatacc gctacaatta atacatatgc ttgaatgact
    14341 ggaaaatcgt gttcaagtat tgcttttaaa cttaattgac ctagtccagg ccatgcaaag
    14401 atatactcga taacaactag tccacccatt atcattggta tagacataca aaagattgat
    14461 accgcaactt gtaaagcatt acgcaacaca tgcaacatta atgtgataga tttcacaccg
    14521 cttgctctta aataaagtac ataatcttca tttaattgtt ccaccatcga gcgtctaaca
    14581 tttctaaagt aaataccagc ataggcaatc gtaataacga tcactggcaa tatgtaactt
    14641 tctggacctg ttaatccaga agtcggcaat atgtttaact tcactgaaac gtaaataata
    14701 agtattgaag ctatccaata tgatggtaat gcagttagaa agaaagccac tgaacgtatc
    14761 gcacgatcag tgaactttcc tctttttaat gcactaacta cacctaaaat aattgatgta
    14821 atcatcacca taacacttga aattattgtt aatttcaatg tattcataaa tgctggacca
    14881 atacgttcag caactgggtc acctgtaatg tagcttgtac caaaattaaa ttgcatcgct
    14941 tcaagtaacc aatttttata ttgaattaat aatggatcat tgaaaccgta cttctcattc
    15001 gtttctgcaa tcaactctgg tgttacattt ggcgtccctt gtgcatgtaa aattgtcaca
    15061 gctggatttt catttgtaat atacgtcaat agaaatgtca taaaacttac tacaatcatc
    15121 aatggaaaca tgagcgcaat acgttttaag ataaatttga acatctaatt gctcctttat
    15181 ttatactgca tttcattgaa tggtaattca tactgtgatt gtgtgaatga tactttttct
    15241 aaatcttttg gtgcaacaac tgtcatacta ccgtgtgaaa tagggataaa gataccttca
    15301 tcatcaattt gtttcaaaat gtttttataa gcgtctgaac gctctttacc gttttggatt
    15361 ttaaatgcgt catcaatgct gttgtatatt ttatctttgt tctcaatgcc tgatgttgca
    15421 ctttcataac catttttcgc tttaaatgct gcaatagtac tttgtggatc gtacaataat
    15481 ccccaagttt ggttgaacat taagtcataa tcaccagaag tacgacgttc agcaatttta
    15541 tctgatgttt cgccattgat gtttaactta atacccattt tcttaaattc tgcttgtaag
    15601 tattctgctt gttctttttg acttgaagaa cctttgtcat agtacattgc catttcaagg
    15661 tttttaccat ctttttgacg aacatcgctg tctttacctt tcttccaacc agcttcatct
    15721 aataatgatt ctgctttttt aaggtcatac ttacgtgttg gcatatcgaa attaatgtct
    15781 gttacatttt tcgcaaataa ttgagttgct ggtttttctt gaccatctaa aatttctttg
    15841 gcaattttat ctctgtttac catatgacca atcgcttgtc tgactgtttt gtcactcaca
    15901 gcgttatctt ttttaccaga attgacaact aacattttcg tattcatagg ttgactacgc
    15961 ttaacttgat agtcacctgt atctttcaat tgttttaaag agtctttgtc taagctatct
    16021 gtacctctat catctgtgaa ggcaaagttc gtttcacctt ttttcattga taggaatgct
    16081 gtttcaccag caggcattac ttttgcttgt actttgttaa gtttagactt ttcgccccag
    16141 tattgatcat ttttgttaaa gtctgcagac tcatcttttt tgtgttcacc taatttaaat
    16201 ggaccagtac catcgaactt tttaacgcca tcttttgttg taccgttttt aaagtctttt
    16261 ggagacacaa atacatatgg acgaggcatc gctaattcag ccaatgcagg ttgatatgct
    16321 tctttcaaat tcaattcaac cgtgtactta tctttaactt taacattgtc aattaatgtc
    16381 gaaatcttta accaagaatg caattttttg ttttcttgaa ctgcgtcaat atttttctta
    16441 actgcgtcag catcaaatgg cgtaccatca tggaatttaa cgtcatctct caaatggaac
    16501 gtgtatgtct tcccatcttc agacacatcc cactttttag ctagtaaagg cttaatacca
    16561 tctttcgtgt tacgtacaag cggctcgtat atcatacttt cagcagacat tgatccaccg
    16621 taaacatgcg gattcatatc accgatatct ttaaccgtcg tatacgttaa ttgcttgttt
    16681 tcttttttct cctctaaacc tttattaccg ccacaaccag ttaaaattag ccctgatgca
    16741 agtaacattg cactcatttt agttagtttt ctcatttgct tttcctcttt ctaaattgat
    16801 aagttgcatt acttaatcaa atcgtaatga ttatttttct caattatttg cttattttta
    16861 tgtaaatgtc aaacataatt caataatttt tttctattat aaaacttcaa acccttatta
    16921 tgaaagcgtt ctattgattt ccaaaaattt tgtgacgagt gctttatctc cttcaaatag
    16981 atttgtatta aattgagaag ataagtgttg atcttgatgc atatccttgt acgcctggca
    17041 acttgcttca tagcgagtta ggaactgatc aatcattggc gttttgatac ctagcatttt
    17101 cccaatatgc tgaatcatcg ccgttctgta ataatcttca cttggcattc ttggaatttg
    17161 aacaacatcc tgttcatttt tatagacttg cttaaatggt acagctgaaa aatcaaagta
    17221 atgtccgttt tcgtctggct gtgaaaatgg atcaatgagg attgcggtat atcttacata
    17281 aagcagatat tcttgtaaga tatctggcaa gatttcgaaa tgctcaatat caccttcatc
    17341 caaagtttca ggacgtactg gataattttc cttcaccata aattgaagca ggttgactga
    17401 cggcactcta aatgcttgta aaataaccat catttccttc cacattaaac gcatttcacg
    17461 gattagtgtc atcgttatcg gtccttcagg aaataactta tacacataaa ccggtacatc
    17521 tgttccttcg aaaatggctt tcaatgaaaa gtcattcata aatagtggtg ggtgcacata
    17581 aagcgaactg tttcgcgttt cagcatgcag tggcgattca acgacttcta attgaatctt
    17641 caattgctca gctaaagcag agattcgttg acacattgtt gagtttgaat gtgtcgatcc
    17701 catgtacaat ttctttttta cacctgttgt caacacatga ttaggcgctt ctttatcaac
    17761 aatacgtgta tcgccaagat aagttgagaa tgaaatcact tcgatatctt tattaaattt
    17821 agacataaat tgttcgacaa tcatttgcga accaaatgtc ggtgatatta aaatgacatg
    17881 tttgacactt tgcaaagttt ctaacgacaa ttgctgtagt gtgtcataat aagcatctgc
    17941 tgtgcatgcc atgacaaccg tttcgtattc acccttaacg tttttaacat ctttatacaa
    18001 acgattaatt tcaaacttac cttccagatg ttgatgcgcc tcgttttgta ttttgacttc
    18061 aaattgtttc gcttttttat acgcttgata taagcgtttt gatttttctg atgttgaggc
    18121 acgtccaacc atatcaatct cataatctga ttttaaatag caaatattcg ctaattggat
    18181 tgcgaccgga ccagtgccta tcattaataa tttagacatc cgtaatacct acctttatag
    18241 ctgctttttt ataaagtgct atatcaaaaa tttgctgtgg tctcatatgt ttattcacac
    18301 attgccactt atcttccgct gtttcttgtg acggataatt aaatattgct ttgatgccat
    18361 caccaaagcg cattgcaact acgacatttt catttgttaa atcatataat tcttctaaaa
    18421 tgctgtactt taaaggaatt gtcgagctga atatgatatg cgtcacatct ttgatatctt
    18481 taagttcaga taccttttga tccgtaattg ttatatcttc atttggtgct aagacgttaa
    18541 cgattctgcg ccctaggtca acggcttgtg gatcaatatc aataccgata actgaagcac
    18601 ctgtttcttt tgctacttga attaacgtca ttggatatgc acctgaacca actaacaata
    18661 atttgtcatt gctagctata ctacattgtc caaactcttc atcaatacaa tgttctatat
    18721 tgtcaaagta acctgtggta gacgcctgtc cgtctaatag acgtctcgct ctaatgactt
    18781 ccacttgttt aacacactgc gctgtaatat attgcagtga ttttattaat tgtgcttttt
    18841 cttcaacgtc acgccaagca ttaaattgtt gttcataaat aggatttaaa ataaattcac
    18901 tatagtcatc catcaatgtt tctaatgctt cgatatattg atcgtcttgt aatacacgct
    18961 cgtaatgcgc ttcaaacttt tctaaatatt gttgtaatat caatttgatt tcattattaa
    19021 agttattcat tgtaggctcc ttattctata tatgcttttc ctgtagctac agttgtaacc
    19081 tgtcctttaa ttgaagtttg atatcccaat tgatgacatc gctttgatgt cactaaaata
    19141 ctgccccctg gctgatgtac tgtaaaatct ttgcatgcgt cattacgttg ataattatta
    19201 aaaaccccaa ttgatgctgt accagaacca cagctatttt cccaaattaa actttgaatt
    19261 tctggtatat agattaatgg ctgtaaaaat tgacgttgtt catcaaaaag catcatacct
    19321 actgttttat atttgtgact ccattgttgc tcacgcacaa acgcttcaac caaatgttga
    19381 atttctgttg ttacttgttt aactggaatc acataatgta catatgtttc ataaataatt
    19441 tctattgctt tccatgaatg attacccata ttaattgttg ttggcacaac acgatgggct
    19501 tgtggcattt gaacttcata gtattggcaa tcatgaattg cgcattgcac taaatccgaa
    19561 cagccagaca ccttcacctt aaactgttgg tctttaagca aatgactttc ctgcaaatga
    19621 tgtatatatg acatcgtcgc attaccgcaa aattcattac cgctcataac taagtgaaaa
    19681 tcattaccat catcattttg tgttgattct ataaagccta cctgttcaca gcatacatgt
    19741 gttgcggcca tcaactgatt ggcgatagat gcatattcac tagcatcatg ttttgaatga
    19801 acaagtatcg tcatattccc cgaaggatta tacttagaaa attctataac ctgccgattc
    19861 atacgacact cctttagatg tatttttaga gcctctgtaa ttttaattac tagaggctct
    19921 tatgcagttg ttgcgaagca aacaactgta ttcctctaaa cctgtaattt tttagaagat
    19981 gaacctaata gctctatcta gtaggtaagt catgctaatt cgcaaaagcg aaaaccatgg
    20041 ctcttatgca gttgttgcga agcaagcaac tgtattcctc taaaactgta attttttaga
    20101 agctgaacct aatagctcta tctattaggt aagtcatgct aacccgcaaa gtggaaagca
    20161 tgacttagta atcggttgtt gtatcaaaat gcaaccgtaa accttattta aagttgcttc
    20221 ttgtggctct acaagcgaat gcagttgtcc gttaactaca ttcacttaga tgcttttact
    20281 gttttcacct caactaaaac ttcaaaaaag ttgcagaaaa caaatcgtaa ctattacgat
    20341 ttgattatat actatattag ttgagaatca ttgtcaataa aggttgtaaa tgaatgcaca
    20401 tttatatttt gataatcctt ccataaactt cgtattctca gcacattcaa gtctttttga
    20461 aaatttttat caataattga gtagaagttc cgttctagtc agttcaaaat tttacgtaag
    20521 ttgtctctgc gtattgcttt agtgagaata acccacactt gtggaaacgt ccttttgtta
    20581 atctacaatg gatacccttg tctttcgcta acatttccta ttaccaatcc tgtaacggat
    20641 tttcatcgtc aagttgttgc ccatacaata cgcaccaaaa aagctttgta caatttcgta
    20701 caaagcttta aaaatttaaa taaaaatatc attgttttaa tgaactagcc atatcagatt
    20761 ttatttcgtt tgctaatact tgtaattcag ggaagtaatt attagcaaag ccaattatta
    20821 ttttattttt tacaaacgtg ttaatctctc gatcttctgg aatcgtattt tctattaaat
    20881 tcaaaagctc actatgactt atgttctcta tacttactgt atttaaatat tttaaaatca
    20941 caagatacaa ttcatcaaat acttcatgtt caaaatcaaa gtccaataat aataaactaa
    21001 cggaattagt ttctaactga ttatctccaa ttttatctgc aatctctctt atagcttgat
    21061 atagcaatct aaaactttct ttttctttta acataaaatt ctccttaatt tattagaata
    21121 gataaaatac agcttatttt aaagatactc caaaagtttg aactcgagat tctgataaat
    21181 tagatgcacg acctacaata cgttaaaaca agctttaagc aatacacttc aaatgaacaa
    21241 tatgtgattt tcaaagatta attaatctcc gctttaaatg tctttcattg cgacaatatg
    21301 catacattgc ctacttcatg ccaaattctt ttccactgga tatcgatttt aaataattat
    21361 tccaatgtcc gcttccacga atcatcactt aacatctcgc caggccatgt gtaagccatc
    21421 acaccaccat caattcgaat cgtctctcca gttatgaatg aactgtcgtc agatgctaag
    21481 aagactacta atttagcaac ttcttctggt ttacctaaac gtcccagcgg agtcatccat
    21541 ttttgatttt ctctaaatgt tttacctgca tcatcctcac tcgtacctgt cagtttatct
    21601 actaacgggg tttcaattgt acctggtgca atcgcattgg ctcgaatgct atcacggcca
    21661 tactcaattg cgattgattt tgtaaaatta atcactgcac ctttcgcagc attatatcca
    21721 gagcgataca agtctgctgc ttgtccggaa aatgaagacg tattaacaat agagccacct
    21781 tgattcatca ttaaaggtaa catcattttc gtcattaaaa atgtcccacg catatctaca
    21841 ttcataatct tgtcatacac atctattgga tactcatgaa ttctatcagc cgcattatcc
    21901 acaccggcat tattaaacaa cacatcgatt cttccaaact gttcctttat gtcagacaca
    21961 aagtctacca cttgttgttc gcttgcatta tccacattat acgccttcgc attgtcacca
    22021 ttacttttaa ttttatcgac agtctccgat accgcttcag ctatgtctac cgccaataca
    22081 tacgcacctt attgagctaa agcgattgca gaagcttgac cgatacctgt acttgctcct
    22141 gttacgactg ctactttatt ttccaaacgt ttcattcagt cactcctata caacatatct
    22201 taaacttaca gtccttattt tccactgttc gatgcttcat aaacctaata gataagtctt
    22261 caggcagtct taactactct gctatacact ttctattgaa taactcaaaa tatttaacaa
    22321 tattagtttt tttattttct gaaaattgtt acttttgaga gtattttttg ttacacttta
    22381 aatatttaac aaagggggga ataattgatg acatcaaaac atcaacaaaa ggggtctatc
    22441 gtcaatcgtt tcttgaatag tgttgaaaaa atcggaaata aattgccaga tcctagcgtc
    22501 ttattctttt taatgtgtgt gggcttagcc attatgacat gggttatctc attatttaat
    22561 gtatctgtta agcatccagg tacgcatcaa accatttata ttaaaaatat aattagccat
    22621 gatggattta cgatgataat gaatgatacg attaaaaatt tctcagagtt cccagcatta
    22681 ggcttagtac tagcagtgat gattggtatt ggtgttgcag agaaaacagg atacttcgat
    22741 aagttaatga tttctgttgt gaatcgcgca ccacgcttct taattttacc gactattata
    22801 ttaattggta ttttaggtag tacagccggc gatgctgcga caattatctt gccgccgctt
    22861 gcagcaatgc tttttattaa aattggctat caccctatcg ctggactaac gatggcatat
    22921 gcttccgctg ttggaggatt tgcagcaaat atagttgttg gtatgcaaga tgctttggtc
    22981 tattcattta cagaaccggc aacacgtatc gtttcagatt ctattaaaac aaacgttgct
    23041 atgaactggt actttatcgc agcgagtgtc gttgtattac ttcctacgat attgctagtt
    23101 acaactaagc tcattattcc tagattagga aaatatgatg acagtttaat gcatgatgac
    23161 catgaagaaa catcttcaca tattactgac aaagaagcac atgctttaaa atgggcaaat
    23221 atcagtttca tagtcacaat tattttatta attattacag ccattcctga acatagcttt
    23281 ttaagaaatg ctaaaacagg cagcttacta gacgatgcgc cattaattaa cggtgtcgga
    23341 ttaattattc ttgtcgtatt tttagtacct ggattagttt atgggatttt aagcaaagaa
    23401 attaaaaata caaaagactt aggtaaaatg ttcggcgatg ctgtaggatc aatgggaaca
    23461 tttatcgtta ttgtgttttt tgcagcacag ctacttgcgt atttaaagtg gagtaattta
    23521 ggaattatcg ctgctgttaa aggtgccaaa ttattagaac atcaaaacgg tattgtattg
    23581 atactaggta ttattgtact gagtgcaatg gtcaacatgt taatcggaag cgcatcggct
    23641 aaatggggta ttttaggacc gatattcgta ccaatgttaa tccttatcgg ctttcatccc
    23701 gcattcacac aagtcattta ccgtgtaggc gattcaatta ccaatccaat tacaccgatg
    23761 atgccgtact tacctttatt attaacttat gcacaaaaat acgataaacg catgaaactc
    23821 ggagccttac tttctagttt aatgccgtat tcaatcgcgc taagtattgt atggacagta
    23881 tttgtcatca tttggttcct attaggtatc cctgttggac caggtggacc gatattcgtt
    23941 aaataacagt attcaaaggg ataaattaaa atgccactat acaagatttg acctgtatag
    24001 tggcatgatt tgttatgata tagcgttcga gatgaaactc tgcatttata tgaacaatta
    24061 tagtctatca ctaataatat ttaatctgtt ccaaccgtta atttggttta caacaaagac
    24121 aagatcaata atttgttctt catcataaaa tgattttagc cggtcaataa tttcaaactt
    24181 cttaaagtct ttgattgaat ttaacatttc tgcaaattca agcgtcactt tttcttgatc
    24241 agtaaataaa tctaaatgtc taaaactcaa gacttcatca attttcattt gtgttacacc
    24301 caattccttt aattcttttt tatggatatc aacacaataa tgacaaccgt taattttaga
    24361 aattaataac ttaatcaatg cactaagttc atcattaacg ggtgattgat gaataaactt
    24421 ttcaccttgg ctcattacag acattaactc tccattttgt ttcttgtaat tatacggcat
    24481 ttgcaatctc tcctttacat tgtttaatac caatccagtg atactacact tcgaacaata
    24541 ttggatagaa tttgaaattc tgtataacat cttgtcaatc tatgctttag ttaaaacatt
    24601 tagcacttat atttaaaaaa tatataaaat atgaaaacag attgaaagga tattatacct
    24661 tgctctaccc ttttttataa aaaatcacac acatttgaga aaatttgata atgtcaataa
    24721 atcacgtcat cgacattgtt attcgatata tattgttctt ataatggcca aactattatt
    24781 ccatttaaat taacgtggtt cgtttgtgtt aatgtactgt acaaaactgg ggaagtattg
    24841 gtgatgatga agtgatttat attaattgcg aaacaccttc gatagcaatg acatagatta
    24901 ttggaagatt aaatcaaaaa taaagaagac cctagtggcc ttctttttta tctaattctt
    24961 ctttgatttt ttctggtgat tttctttcat gtgctggttt taataattta tctactcctt
    25021 cactttttat catagcgcca ccaaattgat aattatgttc aggcgatgca taagcagtaa
    25081 aatcatcttc tttattttca ttgatgtatc cttcaatcac agcacttccc ataggacttg
    25141 tttccaagtt tgtgaaatgt acagatttaa tcgtgttcgt ttctttagta ttatgcttaa
    25201 gatagagtgt aatacgttct tgttgttctt tgtagtaaat cgctttttgt ttttcttttt
    25261 catcatattt cattttaaaa tgcatgaccg tagaaactgt acatatacac aatatgacac
    25321 caataattat aaaaacatgt tttttggaca ttaaagtaac tcctaacttt agtgactctt
    25381 ctttttattt agttcttttt ttatatcatc tggtgatttt aactgattgg ctggctttag
    25441 taactcgctt aatttttcac tttctatcat agcaccacca aattgataat tatgttctgg
    25501 tgtagcataa gcagtaaaat tatctttttt gttttcattt atgtaacctt caatcacagc
    25561 acttcccata ggacttgttt ccgagtttgt gaaatgtaca gatttgattg tattcggttc
    25621 tttagtgtta tgcttgagat aaagtgtaat gcgcgcttgt tgttctttat agtatattgc
    25681 tttttgtttt tctttttcgt catatttcac ttttaaatag atacctgcaa cactaattaa
    25741 tatgattaac ataatactag ttaatattaa aattttcttt ttcgacatct atcaccagct
    25801 cctaaaatgt ggtggaattt tatacagttt acatatttat gatactacaa aacacactct
    25861 tacaaagaaa atctataatt ttaatctagg tcaaggaaaa gcagccaaaa gtgtttatat
    25921 aagatcaacg aaatattgcg ggtataaagt ggttaacaga taaatacagt ggaaattgta
    25981 ctaaaaattc aacttttcat ttttatatgt aagtatgatt aacttgaaaa caaaattaaa
    26041 acggccgcat agttttagcg gtcgttcttt ttagactcta attccttttt tatctcatca
    26101 ggtgatttgg tttgatgtac tggttttaat aacgtgctta aatttttact tttgattaaa
    26161 cttccaccaa attgataatt atgttctgga gacccatatg caacaaaatc atcttcttta
    26221 ttttcattta tgtaaccttc aattactgca tcgcccatag gtcctctttt taaacttgtg
    26281 aaatgtacag ttttgatggt attgggttct ttagtattat gcttaagata gagtgtaata
    26341 cgctc
//
LOCUS       DALAGF010000028        25710 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.028, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000028 DALAGF010000000
VERSION     DALAGF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25710
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.028"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(161..1165)
                     /locus_tag="O2692_001099"
     CDS             complement(161..1165)
                     /locus_tag="O2692_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCZ4298735.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
     gene            complement(1149..1859)
                     /locus_tag="O2692_001100"
     CDS             complement(1149..1859)
                     /locus_tag="O2692_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HCZ4298736.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGEKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            complement(1947..2153)
                     /locus_tag="O2692_001101"
     CDS             complement(1947..2153)
                     /locus_tag="O2692_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298737.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            complement(2219..2827)
                     /locus_tag="O2692_001102"
     CDS             complement(2219..2827)
                     /locus_tag="O2692_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HCZ4298738.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESKFETIVSPLPEKSSFTAKFQVLPKVVNPTFIKNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            complement(2827..3978)
                     /locus_tag="O2692_001103"
     CDS             complement(2827..3978)
                     /locus_tag="O2692_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HCZ4298739.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHAMCTHSLAKDDIYAAAYSFEKAESINSTYEHQT
                     SYVSKYHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKNNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            complement(3962..4555)
                     /locus_tag="O2692_001104"
     CDS             complement(3962..4555)
                     /locus_tag="O2692_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCZ4298740.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIR
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
     gene            4906..5574
                     /gene="queC"
                     /locus_tag="O2692_001105"
     CDS             4906..5574
                     /gene="queC"
                     /locus_tag="O2692_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCZ4298741.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            5576..5995
                     /gene="queD"
                     /locus_tag="O2692_001106"
     CDS             5576..5995
                     /gene="queD"
                     /locus_tag="O2692_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCZ4298742.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            5999..6712
                     /gene="queE"
                     /locus_tag="O2692_001107"
     CDS             5999..6712
                     /gene="queE"
                     /locus_tag="O2692_001107"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCZ4298743.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
     gene            6811..7395
                     /locus_tag="O2692_001108"
     CDS             6811..7395
                     /locus_tag="O2692_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298744.1"
                     /translation="MIVYILINIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLDETAENNNKIKKLMIKGLIIINI
                     SFIVMMVLTPYWYLYLIVYLIFLLFLLWQKVYKF"
     gene            7675..8115
                     /locus_tag="O2692_001109"
     CDS             7675..8115
                     /locus_tag="O2692_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HCZ4298745.1"
                     /translation="MNTKYFLAAGAVITTLALGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            8457..8930
                     /locus_tag="O2692_001110"
     CDS             8457..8930
                     /locus_tag="O2692_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCZ4298746.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            8905..9591
                     /locus_tag="O2692_001111"
     CDS             8905..9591
                     /locus_tag="O2692_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4298747.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            9591..10646
                     /gene="saeS"
                     /locus_tag="O2692_001112"
     CDS             9591..10646
                     /gene="saeS"
                     /locus_tag="O2692_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HCZ4298748.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCNEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENEDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(10718..11701)
                     /gene="csbB"
                     /locus_tag="O2692_001113"
     CDS             complement(10718..11701)
                     /gene="csbB"
                     /locus_tag="O2692_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HCZ4298749.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSLENDEKDTHKVYSK"
     gene            complement(11833..12672)
                     /locus_tag="O2692_001114"
     CDS             complement(11833..12672)
                     /locus_tag="O2692_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCZ4298750.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            complement(12894..14243)
                     /locus_tag="O2692_001115"
     CDS             complement(12894..14243)
                     /locus_tag="O2692_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCZ4298751.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(14464..15645)
                     /gene="nagA"
                     /locus_tag="O2692_001116"
     CDS             complement(14464..15645)
                     /gene="nagA"
                     /locus_tag="O2692_001116"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCZ4298752.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEVDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDNALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(15953..17911)
                     /locus_tag="O2692_001117"
     CDS             complement(15953..17911)
                     /locus_tag="O2692_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCZ4298753.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKAGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFNPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YAFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(17917..18837)
                     /gene="pfkB"
                     /locus_tag="O2692_001118"
     CDS             complement(17917..18837)
                     /gene="pfkB"
                     /locus_tag="O2692_001118"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCZ4298754.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(18834..19598)
                     /locus_tag="O2692_001119"
     CDS             complement(18834..19598)
                     /locus_tag="O2692_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCZ4298755.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(19848..20330)
                     /gene="ybaK"
                     /locus_tag="O2692_001120"
     CDS             complement(19848..20330)
                     /gene="ybaK"
                     /locus_tag="O2692_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCZ4298756.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(20504..20956)
                     /locus_tag="O2692_001121"
     CDS             complement(20504..20956)
                     /locus_tag="O2692_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298757.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            complement(21249..22415)
                     /gene="norA"
                     /locus_tag="O2692_001122"
     CDS             complement(21249..22415)
                     /gene="norA"
                     /locus_tag="O2692_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HCZ4298758.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANDYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            22624..23046
                     /locus_tag="O2692_001123"
     CDS             22624..23046
                     /locus_tag="O2692_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCZ4298759.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
     gene            23232..23849
                     /locus_tag="O2692_001124"
     CDS             23232..23849
                     /locus_tag="O2692_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HCZ4298760.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYF
                     TYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVY
                     FFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            23846..24130
                     /locus_tag="O2692_001125"
     CDS             23846..24130
                     /locus_tag="O2692_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298761.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(24285..25658)
                     /locus_tag="O2692_001126"
     CDS             complement(24285..25658)
                     /locus_tag="O2692_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCZ4298762.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVTAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYNVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
BASE COUNT         8468 a         4625 c         3634 g         8983 t
ORIGIN      
        1 ttttagtcaa aattcaagta aaaacacact ttatcttttc attttactgt gaaacgaatt
       61 agaaaatgat acatttcgca gataaaacac agtattattt cattatacat taggaggaaa
      121 ttatttgcat cggactcgag tatgagctac gtaccaaata ttatttcatc atttctaatt
      181 gcattctata ttctattcca tggaataatt ggtttaacca gttactatac ttaacaaggt
      241 tcttttcagc cgttaaaata tctataaatt gaaatttcaa acgtgatcct tgcggttttt
      301 gtgctaattt cgttaaatga taacttgcga ttgtaccgat ttgcgggtag ctacctagcg
      361 tataatgatc atttaatagg ataataggtg tgccatcacg cttaatttgt atcgttcctt
      421 ttttgacagt ctgatacggt ggcatatctt cataaaaagc tttgattttt tcaccttcaa
      481 gcatcatccc catgcgattt gctttgctcg ttactttata atcatgtttc acaaatcttt
      541 gaatggcatc ttctttaaaa tcttccgtac ctttattttt aacgacatga aatacatcag
      601 acatataatt aaatgacaag gcgtatccat caatacccca atctgtttgt ttcgtgtgag
      661 caaggttttc aaataactta tgatgacgag ctgtataatc tctcttaagc tttatttcat
      721 cgccatcttg taatgttcta cctttaaaac caccaatttt tacattaaag tcggttgagt
      781 tagatcctaa ccatgcatct aattcaaatc cgcctcccac agctaaatac actcgcgatg
      841 tataacttgt ttctctaaac tttaaaacat cgcctttatc taacaaatac aatttatatg
      901 gagatatagt catatgctca gtgtaagctt tgacattacc ccctgctaat gcaatcagcg
      961 taggttctgt aaaacgaatc gttgccattt tattagtcat ttccaaagtt gcttcattct
     1021 tgtcatttgc aactaatcga ttagcaattt catgtgctaa agtatcaagt gcaccacatg
     1081 gaattacacc atcatgttca tatccccttc tgccaaagtc ctgaaagcta ctgaataagc
     1141 cacttttttc aattatgatt gacatcttta aacgctccta cttctaattc attttcattg
     1201 ataggtctaa atttaatatt atcgcctaac tttaagcgtg cgaaatctga ttctttcgga
     1261 ttaaaaagtg ataatggtgt ataaccaata actaaccaat cattaattgt atccgtagtt
     1321 acaataccgc atttttcccc ttcaagtact acagaaccag ctggaatgaa ttttttctgt
     1381 ttactcgtgt gattaatata caatttctta tttaatccag ttaaataagg aaacccaggt
     1441 gaatatccca tcatcgaaac aaaatattgc gccttagaat gtaattcaat aaaagtttct
     1501 agcttgattt tgtaatgttt taaaagtgat tctaaatccg gaccatattt cgaaccatac
     1561 acaattggta tttcaatcaa atcttgctgg ttaaaatcat gttttatgtt taaatgaatc
     1621 gattctatta gtgctttcat gtataaaaaa ggtgattgta tattatggtg tttaatcata
     1681 tcccttgcgt catagacaat catcatgtct gattctgatg gcacaatttc tataataaat
     1741 ggataatttt gttcaattaa atatgagcga agtgttaata aatcttcggt taaactttta
     1801 gatacttctt tttcaattgc gactacaatg gcttggtcac cttgactata tattttcatt
     1861 tgtacacctc ttcaatattt ggttacttta ctttcatttt taattataga atcgattgaa
     1921 tagttcattt tacctactca ctaaatttac tggtggtaca ttaaatccat tgcccgcaca
     1981 acaaaaggca taagttcgtg aattaatgcg tatacaagga taaagcttat aacagtagta
     2041 attgttgcta tcaaacgaac aacatatatt ctattttcag atagcaatcg attcactaat
     2101 cgataattta cgtatacaag aattaacaac agcacaatat aaacaataat catatttggc
     2161 cccatttaat ttttaaattt gttttcacat cattttctac tttattatca tactaatttt
     2221 aaaggcaaag gtggacatcg gcaacctctc gtaaactatt tatcaaaaat aaacgtatct
     2281 cattgttatg atatttataa atcaattcgt ttttattaaa gtctttttca acaagtttgt
     2341 cactatctat taaataatca cgcatgcatc cttttaagaa atcatcttta taacttggtg
     2401 tgtaccattt tccatcctct tcaatgacaa tgttgccaat atcaaattca aggaccttgc
     2461 cgtcctctga agttagcaat attaaatctg tttcatgatt gtgtgctaaa tgctttcgtt
     2521 ctgtcgtttt attttttata aaagttggat taactacttt gggcaacact tgaaattttg
     2581 ctgtaaaact acttttctca ggtaaaggtg acactatcgt ttcaaatttg ctttcagcat
     2641 ttaatacgat tttaagtcta tattgaccac tgtgatactt tgttgttaca tcgtttaatt
     2701 catttcgcca tgcatgttca tcaaatttaa agtttaatcg ctcagaagaa cattttatgc
     2761 gattagtatg ataagtaagt ctagggatat gtccattatc aattttcatt gtttcaaata
     2821 attgcattat aacatctcca aaatcttggt ttttgcgtaa aattcattca cttcatcttt
     2881 tggcttagaa tcaattgtaa tacctgctcc gactccataa atcgcttgtc catatttata
     2941 ctcaatagtg cgaatcggaa tattaaaaat cattttatca tcttcagttg gaagtaatag
     3001 tccaattgct ccgcagtata taccacgagg tgaactttct aattgtttaa tatatttcat
     3061 tgtattcagt ttcggtgcac ctgtaatcga accacaagga aataacgatg ttaaaatttg
     3121 agttaagtct gtattatttt ttaaagttcc acttaccatc gaagtcattt gaaatacagt
     3181 tttatatgcc tcaataaaaa atagtttata caccttaatt gagccactct gtgatattct
     3241 ccctatatcg tttcttagta aatcaacaat catgacattt tctgcacgat ctttcgaaga
     3301 agtttgcaat gttttataat actgttgatc ttcagcttcc gttttacctc taggcattgt
     3361 ccctttcatc ggtttgctta tgataacgtt atcgacattg ttaaattgtc ctttttgaaa
     3421 aaataattct ggtgagatag acgctacttg gatttcatca gtttgtaata acgcagtata
     3481 attaccatta ctaaattgag ttaatcgttc atataaagta ctaataggat aataaatgtt
     3541 atccgttaag cgcgccgtat agttcacttg atacgtttcg ccttcaacaa ttgcttgttg
     3601 gacacgttta atattagtca tcataacctc agaagattca acaaatgaaa aatgatactt
     3661 tgatacataa gaagtttgat gttcatatgt tgaatttatg ctttccgctt tttcaaaact
     3721 ataagctgct gcataaatat catctttagc taatgaatgt gtacacatag catgattaaa
     3781 atactttgcc gcttcgtaac ttaaatataa cgagacatat ctaccttgtc gttgctgcgc
     3841 ttgtgcaaag tgtatcactt ctcccacatc agccaactta gtagcaacat acttctttat
     3901 aaatcccttt aattgaatat ggtattgctt atattcattt tcagttaaat agtagcgata
     3961 attatattct attctcacag taatcaccta ccttcgataa aaataattca acttgtcgat
     4021 aaccgtattc actcaaaata gattcaggat gatattgcac accaaaaacc ggaaatctaa
     4081 tatgctcaaa tgccataata atcgcttcat cgttttttgc tgtaatcttt aagcaatttg
     4141 gaaaagtcgc tccgtcagca attaatgaat gataacgcat tacattgaaa ttttgaggca
     4201 gtccttgaaa aataccttca ttggtatggc gtaactgtgt agtatgtccg tgtacaggat
     4261 gatagccgtg aatgatattt ccaccaaaat aagacacgat acattgaaat cctaaacata
     4321 cacctagtat aggtacacgc tgataaaatt gttctaacac ttcattcaag ataggataat
     4381 catccggatt acccggccca ggcgaaataa caattgcttt tggcttcata ttaatgacgt
     4441 cttctatcag cagattatca ataccaacaa cttgaactgt tagtttcgtt tgagtcttaa
     4501 tatagtctat taaattatat gtaaatgaat cattattatc tatgactaga atcattgtat
     4561 actccgttct aaatgtgttt tatttttata atatgtattg gatgtagcta aaactttaaa
     4621 agcattgtca ttatcctgac cttgatttta actaatatat ggtatattct attcatcgta
     4681 cataaatgaa tatcagaggt tcctagctga aaccctctat aaaaaactag acattgaaat
     4741 ttcaaacatt cgttggtaaa cctttaatat atattatagg agcaacccat ttctttcgtc
     4801 tatcttttat ggagataggc tttttttatt ttataaatta aaaacgggct tatcaatgcc
     4861 attaacctct ttattaatat tattaatgat aggagcaatt acaaaatgga aagtgtatta
     4921 aataatgaaa aagccatcgt tgtctttagt ggtggtcaag acagtacaac atgtctcttt
     4981 tatgcaaaaa aacatttcaa agaagttgaa ctcgtaacgt ttaattatgg ccaaagacat
     5041 gatactgaaa ttgaagtcgc aaaacaaatt gcacaagatc aaggaatgaa acatcacgtt
     5101 ttagatatgt cattattatc acaacttact ccaaacgcat taacacaaca tgatatggaa
     5161 attactaata atgaagatgg tatacctaat acatttgttc cagctagaaa tttacttttc
     5221 ttgtcgtttg caggcgctct agcttatcaa attggggcta agcatattat tacaggcgta
     5281 tgtgaaacag acttttcagg ctacccagac tgtcgcgata gttttataaa atcaatgaac
     5341 gtaacattaa gcctagctat ggacaaagat tttgtcattc atactccttt aatgtggtta
     5401 aacaaagcag aaacgtggaa attaagtgat gaactcgaag ttttagatta tattcgtaca
     5461 aaaacattaa catgctataa cggtatcatt ggggatggct gtggtgaatg tccagcttgt
     5521 catttacgtc aacgtggact aaatcaatat cttgaaagta aaggagcgct ttaaaatgtt
     5581 acaacaaatc tatcctagta caacgcatcc atatcaattc gaattaaata aagattttaa
     5641 tttttcggct gcacatcaca ttccatgtga agaagcaggt atttgtcaaa atgtccatgg
     5701 tcatacttac tttgttaatt taacaattgt cggtgataaa ctagatgaca ctggcttctt
     5761 agtgaacttt agccatttga aaaagatgat acacggtaaa tttgaccatc aactgttaaa
     5821 taacttacct gcttttaaaa acaaaatccc ttcaactgaa atcgtagcgg aaacaattta
     5881 tcaaattgtt aaagaaaatt tggcatcgct cgaacaccaa ccaaaatgta ttcaagtatt
     5941 tgtaagagaa acaccaacaa gttatgtcgt atttagacca aaggaacagg tgtaactaat
     6001 ggctaaaatt cctgtactcg aaatattcgg tccaaccatt cagggtgaag gtcgagtaat
     6061 cggtagaaaa acgatgtttg tgagaacagc tggatgtgat tatcgctgta gctggtgtga
     6121 ttctgcattt acttgggatg gtagtgctaa aggcgatata aaactcatga ccgctgaaga
     6181 aatttatgat gaattaaaac gaattggtgg cgatttattt aaccacgtta caatttctgg
     6241 tggtaaccca gcattaatta aaggtatcca agagttagtt gacttatttc aagataaagg
     6301 tattttcagc gcactggaga cacaaggcag taaattccaa ccttggatga cacaaattga
     6361 tgatttaacg attagtccaa aaccaccaag ttcaactatg acacctgatt taaaaaagtt
     6421 agatgaagtg attacacaat gcgtaccttc atcattaaac ttaaaagtag ttgtattcga
     6481 cgataaagat tatgattttg ccaaaatgat acaccaccgt tatccagata ttcccttcta
     6541 tttacaagtt ggtaacccat atttatcaga cagcgtagat aatcataccg aaaagttgtt
     6601 agaacgctac gagcagttgg tggatttagt tatgcaaagt aatgatatga atcacgttta
     6661 tgtgttacca caacttcata cattactttg gagcaataaa aaaggtgtat aaaggataac
     6721 ttattttact ttttgcaagt catatctaat gaataacaat atattaattg atataatagt
     6781 tgattgaagt ataacgaaca ggagtccatc atgattgtat atatattaat taatattgct
     6841 atcgtcgtac ttataactgg attcaattta tataggcatc aaatgcaaca tttatcatta
     6901 agtgcaatgt tgttatctat tacgataaac gcctttataa atacattcat aattgataaa
     6961 tataacttta ttactctgtg tactataacg atgtttatca tttggacgat actacagttt
     7021 tacattgata aaaaattaaa acctgtatac attacagacc aaaaatttat tgctatcata
     7081 ttaacgattg ttgtcagttt aacgcaacgt gttaccgact tttcaagtac acaatcaata
     7141 tatatgtcta ttccattttt agcccctgcc attttcatca ttggtggtat tatgttgttt
     7201 atttcaacat ttaatagctt agatgaaact gctgaaaata ataacaaaat aaagaaacta
     7261 atgattaaag gacttatcat tattaacatt tcatttatcg ttatgatggt tttaacacca
     7321 tattggtact tgtatttaat cgtctatctt attttcttgt tgtttttatt gtggcaaaag
     7381 gtttataaat tttaatacca aaactattaa acacttctga tattcttagt tcaaaatatc
     7441 agaagtgttt tatagtgtta tctagttcag ataaatattt ccttacttaa aaaaacgccc
     7501 tcctcttatt ttgaccccta tttatttaaa tcagacaatt attttcattt tcaaattatt
     7561 ctttcttcaa tattagttaa gcgatattta aacgaagtta agaattagtt aatggcatat
     7621 tatttgcctt cattttaaac ttaacttatc aaattgaaga aatgaggagt tagcatgaat
     7681 acaaaatatt ttttagcagc tggtgctgtt atcacaacat tagctttagg tgcttgtggt
     7741 aattctaatt cacaagatca aggtaacaaa actgaacaaa aaacaaagtc agaagatagc
     7801 aatgttaaaa ctgataaaac aaaacaccta acaggtacat tcagttctaa aaacggtgaa
     7861 actgttgaag gtaaagctga gattaaaaat ggtaaattaa tgcttactaa ctacaaatca
     7921 tcaaaaggtc cagatttata cgtctaccta acaaaaaatg gcgacattaa aaacggtaaa
     7981 gaaatcgcaa tggttgacta cgataaagaa aaacaaacat ttgatcttaa aaatgtagat
     8041 ttaagcaaat atgatgaagt aacaatctat tgtaaaaaag ctcacgtcat cttcggcggc
     8101 gctaaattaa aataatcaat ttctgagtta aacttttatt tacaacatac tattactata
     8161 cattacaaat ttttaaaata tacattacac tcattactca atggaaagcg tatgatttca
     8221 cagcccccct agcttgtaga aatcatactt tccttttttc aatatatata caactattaa
     8281 atcccataag attgcagagc acataagtaa atttttttag agcttgaggt ttgtttagct
     8341 taagcaaccc atgagctcaa acacttcctg ttcacataac actacaaatc gcattatgtt
     8401 gcttaatctt atgtttatat aaattacaca caataaatag aaagaatgtg aacatcatga
     8461 ataaattatt gctactcgtt acatttatca ttcgtgtggg ttcaggtatt gttatgttaa
     8521 tgcaaggcta cgaaaaatta acgggcggat ttacgctgaa aggtttagta ccagtcatcg
     8581 ctaacaatac tgattcacca gagtggtata agtggttttt cgcaaatata gttgcacata
     8641 cgacgtcatt atttgatatt gttgtcccac tcggagagat tgcaattgga ttaggtttaa
     8701 tttttggagt ttttgcatat gctgctagtt tctttggagc ctttgttatg ataaattata
     8761 tcttagcaga tatgatattt acgtatcctc ttcaattaac tttctttatc cttttactaa
     8821 tgagtcactc attgttaaaa cagatttcac ttaaagaaat cattaattac tttagaggtc
     8881 gtaagaacag aggtgaaaaa atagatgacc cacttactga tcgtggatga tgaacaagac
     8941 attgtagaca tttgtcaaac ctattttgaa tatgaaggtt acaaagtaac aacgacaact
     9001 agcggtaaag aagcaatttc tttactatca aatgatattg atatcatggt acttgatatc
     9061 atgatgccag aagttaatgg ttacgacatt gtcaaagaaa tgaaaaggca aaaattagat
     9121 atccccttta tctatttaac tgccaaaaca caagaacatg ataccattta cgccttaact
     9181 ttaggtgcag atgactatgt caaaaaacca tttagtccaa gggaactcgt tttacgtatt
     9241 aataatttac ttacaagaat gaagaaatac catcatcaac cagttgaaca actgtcgttt
     9301 gatgaattaa cacttattaa cttaagtaaa gttgtgactg taaatggtca cgaagtccct
     9361 atgcgtatta aggaatttga gttattgtgg tatttagctt ctagagaaaa tgaagttatt
     9421 tctaaatcag aattacttga aaaagtttgg ggatatgact attacgaaga tgctaatacc
     9481 gtgaatgtcc atatacaccg tattagagaa aaattagaaa aagagagctt tacaacatat
     9541 accatcacaa ctgtatgggg attaggatat aaatttgaaa ggagccgata atggtgttat
     9601 caattagaag tcaaatcatt attggcgtcg tatcgagtat actattaact tcaactattt
     9661 tagcaattgc atatatttta atgtggttta acggccatat gacactaact ttgaccttaa
     9721 cgacaataat tacaagctgt ttaaccttat taatatgtag tatttttatt aatccactta
     9781 tacaaaaaat taagcagttt aatataaaaa ctaagcaatt tgctaacgga aattacgcaa
     9841 gcaatgataa aacgtttaat tcaccaaaag aaatttatga attaaatcaa tcttttaata
     9901 aaatggcttc tgaaattacg caacaaatga atcaaattaa atccgaacaa caagaaaaaa
     9961 cagaactgat tcaaaactta gcccatgatt taaaaacacc tttagcaagc attatttcat
    10021 attctgaagg actacgtgat ggtataatca ctaaggatca tgagattaaa gagtcatacg
    10081 acatattaat taaacaagca aacagattat caacattatt tgatgatatg actcatatta
    10141 tcactttaaa tacaggtaaa acatatcccc cagaattaat acaactagac caattacttg
    10201 tatcaatatt gcaaccatat gagcaacgta tcaaacatga aaaccgcaca ttagaagtga
    10261 atttctgtaa cgaaattgat gcattttatc aatatcgaac gccacttgag cgtattttaa
    10321 caaacttact tgataatgcg ctaaaatttt caaatgttgg tagtcgcatt gatattaata
    10381 ttagtgaaaa cgaagatcaa gatactatcg acattgctat tagcgatgaa ggtattggca
    10441 ttataccaga actacaagaa cgtatattcg aacgtacatt cagagtagaa aactctcgta
    10501 atacaaaaac gggtggttct ggattaggct tatatatagc taatgaactc gcgcaacaaa
    10561 ataacgcaaa aatcagtgta agcagtgata tagatgtagg aactacgatg actgtaacat
    10621 tacacaaatt agacattacg tcataatccg atttatttat aaaataaaat gcaaagacta
    10681 aaaagaagct cccattaatg agggcttctt tttttgttta tttagaataa actttatggg
    10741 tatccttctc atcattttca agacttgaaa gatttgtagc ttgaataata tatttaggac
    10801 gtgccttaac ttcataatat atcctgccaa tatattcacc tacaacacca attgaaatta
    10861 actgtatgcc gcctaataat aaaatagctg caatcgttga aaaatatccc ggaatattaa
    10921 caccagatat cataatattg atgaataaat agataatata taacacgctt attgaaaaga
    10981 taaacaagcc aagataaatc atcattctca aaggtttact attaaaggaa atcaatccat
    11041 caataccata attaaataac tttttaaagg accacttaga ttcccctttt tgtctctcaa
    11101 cattttgata cgtaaacact ttagtattat agcctatcca ttcaaataac ccttttgaaa
    11161 atcgattata ttcttcaagt gatgcaatgg atttaacagc tctttggctt aaaagtctaa
    11221 aatcaccaac accatcatca aattgtactt cttctacaaa gcaattaact aacttataat
    11281 acaaatggct taatgttttg cgactaaaat tttcaccact tctatcacgc tttgcaatca
    11341 cttgatcata gccttccata aaaccttcaa ccattttcgg aatatattca ggtggatgtt
    11401 gcaaatcaca atctatcatg atgactgcat caaattcagt actatgctgg taacctgcaa
    11461 tcatagctgc ttcttttcca aaatttctac taaaagaaat aaagctgaca tgcctatcta
    11521 ttgtggcaag atgttgcatt tcatctatag tggtatccgt actaccatca tctatgaaaa
    11581 gcatattata atcatagcct ttcacactac tatcttgtga aagtatttca gttaattgtt
    11641 gatgtgtttg tgtaatgact tccccttcat taaaacaagg aatgacgact ctaattttca
    11701 tctatgtcca tcctttttat ttaaaagatt ttgaatgtac ctgagtattg ttttatcaaa
    11761 tagtaaaaac caactcattt attttaatgt acacattaaa aggcattcat aaacgtaaaa
    11821 tttaacatcc agttatttta aatccccaat tttcacatca tctggatttt taccttgtct
    11881 tgcatttcta tttaaagcat caatttcagc tacttctgtt aattctaaat taaaatctaa
    11941 aatatctata ttttcttgaa tgcgtttagg tgtctgagat tttggaataa taattctatt
    12001 gtgtgctaaa tgccaacgta atacaacttg tgctggtgtt ttatgatatt tttcagcaat
    12061 tttaacaatg acagggtcgt ctagtagtcc tctatttctc atcaaaggca tccatgcagt
    12121 cactttaata tcgtgacgat cacaaaattc ttgtaattct tgttggttaa aatatggatg
    12181 tacctcaatt tgattcacca ttggtttgat acttgattga gccattaatt tttctagatg
    12241 atgaacatta aaattacata cacctattgc ttttacctta ccttgctcgt aaagttcttc
    12301 catagcttta tatgtttcta aaaatagacc atctgcttca caaggccaat gtattagaaa
    12361 taaatcaaga taatcagttt gtaaattttc aatcgatttg ttgaaatatt cgaatgtttt
    12421 ctcataacct tgatagtcat tccataactt cgttgttata aacaaatctt ctctatcgac
    12481 gccattatcc tttaatgctc gtcctagtga agcctcatta tcataaaagt atgctgtatc
    12541 aaacgctcta tagcctgcgt caattgcagc atttacaact ttagtcatat cttcgtcaga
    12601 gattttataa acacctaacc caactgaagg catcgggtat ccattattta atatttgtat
    12661 ctcattcaac atctttatat ctctccaatc tatgtatctt ttatatcttt acattaccct
    12721 aaatttttca acaaactcaa ttaatacgaa ttatcgcttt caataaaaat tattcattaa
    12781 atcattaaag atattgagtt ccaatactat tttcactttt cattaatttt agtcaaaaaa
    12841 ataaccaacc aaaaatgaat taaatcattc ttagttggtt tatatattaa tatctattct
    12901 gatttttcat cttcatcaga ctgtccgata gtaggtctcg cttcattaaa ttcatagttt
    12961 aatatcaccc aaataatttg gtggttatcg atttctgaaa caacccagcg atcataagtt
    13021 gtatccacgt aatcatcttt ttgtaaattg gtattacgag attgtaacca tccacctatc
    13081 gtatcaatat cctcagagtc atcaaattct ataccgaact cttcagttaa atcatccaat
    13141 agtactctgc catttacttg gaatgtctta ttatcaattt taacgatatc attcacttca
    13201 tcatcatcaa attcatcacg aatttctcca acgatttctt ctaaaatatc ttccatcgtt
    13261 aaaatacctg ccgttccacc atattcatct ataataagac tcatatgtac atgttcacgt
    13321 tgcattctaa ttaatgcatc actgatacgt gttgtctctg aaatcattgg caactcatgt
    13381 atatagtttg ctattttaat cgtttttcca gaagcgtatt cagttaaaaa ttctttgacg
    13441 ttaataaatc ctttaatgtg gtctttatca ccatcatcag taattggata acgcgtaaat
    13501 tgatgttctt ttattgtttc tagtaattcg tctacattaa aaggttcatt tagtgtaatc
    13561 atttgagttc taggtaccat tatatctttt gcatgtcttt catcgaatga aaagatattt
    13621 tgcatatatg ccaattcagt ttggttgatt tctccaccat tataactatt gttaataata
    13681 attttgattt cttcttctga cattgcatca gtttgggcat caggatttac accaaacatt
    13741 ctaataataa cacgtgcaga accattcatc agccaaatca atggtttcat aatgttaccg
    13801 aaatagaaca atggtcttgc atatactaaa gcaagctttt cagtatgttg aatagctata
    13861 gatttaggcg ctaattcacc aagtactaca tgcaaatacg taacgattat aaatgacact
    13921 gcaaacgaaa tcgtcgtcgt taatgcagtt ggtaaattga ttgcttcaaa tattgggtgt
    13981 aatagctttt caaacgttgg ttcaccaagc caacctaacc ctaaagatgt tactgttata
    14041 cctaactgac aagcagaaag ataataatct agattagcaa tcatcttttt tactatttta
    14101 gcaggtttat ttccttcatc tgctagctgt tcaattcttg ttgctctaat ttttactaat
    14161 gcaaattctg aaccaacaaa tacagtggtt aatgcaatta atagaataaa tataatcaaa
    14221 ctaattatgg tcgaagtttc caattaattc cctatttcta gggattcacc tccatgttat
    14281 atgtgtcact catgggtaac acgcattcaa atttatcact atgacttaaa ttacaactac
    14341 atgttatcgc cttcccattt agacaccccc agaaaaaaat gttattgctt ctattttatc
    14401 ataatataaa gtgcttatgt taacagatta aatctattgc atacatttaa ttatgattat
    14461 ttattagcta aatgtgtgaa ccttgccttg ttttattgta gattttacat tcatatcatc
    14521 atctagaata acaagatctg catccttatt tactttaata ctaccttttc tatcatcgat
    14581 acctaatgca atggcttgat ttaaacttgt tactcgccat aaatgatcta atgtatcacc
    14641 tgtaaatgat attaagttac gtaacccatg attcattttt aaaatactac cagcaagcgc
    14701 accatttgca agacgtgctt gttgcgattg aacagttact ttttgtccac ccaaatcata
    14761 ttctccttca ggcatacctt ttgcacgcat tgcatcggta attaaataaa aacgttcatt
    14821 acctttcata cggtaagcaa ttgcaaccga tgccggatga gaatgagtgc catcaacaat
    14881 catttcggta tgtagagcat cattcaacca tgctgctcca aaaacacctg gttctctatg
    14941 ttggaatggc gtcgctgcat tatataaatg cgtgacatgt ttagctcctc gctcaacagc
    15001 ttcaactgct tcttcgtatg ttgctactgt atgaccaatt gaaaaaataa tgtcatcttt
    15061 atacgtttca agcgcttctt ttgcaccttc aacttcaggt gcaaacgtca taatctttat
    15121 taatccgtta gcagtctctt gaaaatgttt aattttatcg ataaatgggc gtacaacgta
    15181 ttgcggatgt tgagcaccaa ctttattttc agatataaat ggtccttcta aatgtatacc
    15241 tacaatttcc gctgcattgt gaacatcttg ctccgcttca tatttagcaa tatttgtaag
    15301 tgcattatct attttatcag tcgattgcgt cattgtagtg gccaagtatg atgtcgtccc
    15361 ttcagacaac aaattttcgg atagatattt taagccatcg tatgacccat ccattgcatc
    15421 ttgaccataa ccaccatgaa tatgtatatc aataaaacct ggtaatacat gatgaccttt
    15481 agcatcaatc acttgaattt tatttgtcgt atcattatca attgctgctt tatcatccac
    15541 ttctccaatt gcaacaatct gtccatcttt cacatgaatg taaccattat cgattttgcc
    15601 atcttcagta taaactttgc cgttatatat aattaattct gacacgtata aacccttctt
    15661 tatttataaa ttttcacttt aagacactca tattttacta tatctgcttc gtttggcata
    15721 actttttgca aaatattaaa aaagtctata catttgtggt gtctatttct tcattcattt
    15781 atctaaaaat aaaaacactt tcatatcaat tcataaaaac tgaattaata gaaagtgttt
    15841 ttacagcttt gtataagcta ccgttaacac gatatataaa actaacatat attcattgaa
    15901 catacaactt aatatgaagc tctttgctaa caatcatttt acatcattaa aactactcgt
    15961 ccattgattt tgaagcttcg atttctgttt cagttaactt tggtttgatt aaaccgtaaa
    16021 ttaatgctga aactaatgtg ccaactagaa gtgcaataag agtttgaagt aagtgtgcac
    16081 catcagtacc aacaattaca ataataccac catgtggcgc agtaattcgt gaacctaagc
    16141 ctaaagcaat tgcgccacct atacctgaac caatcatcat tgaaggaata acacgtaatg
    16201 gatcggcagc tgcaaatgga atcgcacctt ctgtaataaa tgacataccc atcacatagt
    16261 tagggataat tgaaccacgt tgttcttttg taaatttacg tctaaaaatt aacatcgctg
    16321 tcgcaattgc taacggtgga atcataccac caatcattgc agctgtaatt ggtgctgcat
    16381 taccttcaat caacgcacct gttgcaaaaa catatgccgc tttgttgaat ggaccgccca
    16441 tatcaatcgc catcatagcg ccaataacta aacctaataa tacaatatta gaacctgata
    16501 aattgtttaa tccatctaat aacaaatgat ttaaccaaga tgctggtgga ttaaaggcat
    16561 aaatcatcaa taagcctgta gccgtcactg ttaatagtgg ataaattaat gttggcttta
    16621 atccctctaa tgcttgtggt aacttacgtg tcatggcttt aacaccttgt gttaagtaac
    16681 ctgctaagaa acctgcaata ataccaccaa taaatcctga accacctgaa attgctaaca
    16741 taccacctac aagacctgaa gcgaaaccag gtttatccgc aatactacgt gcaatgaatc
    16801 cagataaaat tggaatgatt aacgcgaatg cacttttact accaatgttc caaagctgct
    16861 ctgcaaacgc attgtactct gagctttttg gattaaatga atttgctcca aataaaaata
    16921 caattgccat taaaatacca ccagatatta caagtggcaa catgttagaa acaccgttca
    16981 ttaagtgttt atagaatgct ttacctgggc ttaatttttc ttgactgtca tttgagttac
    17041 cttttctttg accatcacgg gcaacaaaag gtttacgact tgtatctaat gctttattaa
    17101 ttaattcttc tgggcgttta ataccatctg ctactggtac ttctacgaca tttttaccat
    17161 cgaagcgatc cgtctcaaca tgaacatcag cagcaacaat gatacctgtt gcattttcaa
    17221 tatcttgttc agttaaatgg tttttaatgc cgcttgaacc attcgtttct actttaattt
    17281 taatacccat tttatccgct tgctttttca atgcatcacg tgccatatat gtgtgtgcaa
    17341 tacctgttgg acaagcagtt actgctaaca catatggctc attagattgt ttagatgatt
    17401 gttctgcagt tgcaacttgt tgtgcttcag cttctgcctc ttcttctttt gtcacttcat
    17461 catcagcctc atctatgatc gctagtactt cttcaggtga tgaagcatgt aataatttct
    17521 cacgtacatt ttcatccatt aaaataccag acaacttagc taaagcatct agatgtgttt
    17581 gggcgccacc ttctggcgct gcaatcataa agaataagtg tgctggttgc atatccaaac
    17641 tttgataatc tacgcctgct ttagatttac caaacgcaat agctggtgac ttaactgcgg
    17701 ccactttggc atgtggaatg gcaatacctt cgccgatacc agttgtactt tgtgattctc
    17761 gattgtgaat cgcttcctta aatgacgcga catcacttaa tttacctgct ttgtctaatt
    17821 gatttactaa ctcatcaata acaccatttt tgtcatttgc cattaaatcc attgctattg
    17881 tatcttttgt taataactct gttactctca ttattttcac tccccatcaa gtacgctaat
    17941 cgtaacttgt gattttattt tttctatagc gtcccgtgtt gctaagtcct catcaaatgc
    18001 cgtggcagta ccgcatgcga ctgcttgttg gaatgctttt tcaatcgtta aacctgaagc
    18061 aattccagcc accatgcctg caactgtact atcaccagag ccaactgtat taaccacttt
    18121 cccttgtgga ttaactgctt taatactgat ttctttatca atataaatag caccatcacc
    18181 gccaagcgag acaataacag attgcgcacc tttatcaact aacaaacgac catatttaat
    18241 aacatctgtg tctgagttca ctgttgtatt aaacatcact tctaattcat ctttattagg
    18301 tttaataaat agtggatgat atggtaaaac gctttcagcc aattcttttt cagcgtcgac
    18361 tactaattta gcacctgtct gtgctgtaat ttgtgcaatt tgcgcatacg catcgcttgg
    18421 aatactactt ggtacacttc cagcaacaat aactatatct tcgcttgttg tatttttaat
    18481 ttgttgtaac agttgttcaa attgtgttga cgttatatga ggacccggtg cattgatttc
    18541 tgtttcttgt cctgttttta atttcacatt aatacgtgta tcttcatcaa cttcaataaa
    18601 attcgattga attgcactgt tatttaatgt atctataatg aatttcccag gaaatccacc
    18661 tgcaaatccc aaggcagttg actcaacatc caatgtcttt aagacgcgcg agacattaat
    18721 acctttcccc ccagcgaatt tatatgttgc tgttgctctg ttcaaaccat caattttaaa
    18781 atcattcgta aaaatgacat agtcaattga aggattgaaa gtcactgtat aaatcataaa
    18841 gtccctccta taaagtgata cttttgttgg tattctttta acgattcttg atttaatgct
    18901 ttttcagatg tgatgattgt cgtactttct agcaaaggta cacgagcaaa atatacttta
    18961 ttaaacttag aatgatctat aagtacaaat gattgattgg ctaatgacat tgctgtttgt
    19021 ttaactaatg cctcttgctc atcgggagta gttaatccaa gttcaatatc taatccattc
    19081 atcccgataa aagctttatc gaaacaatat cgtcttaata tctccatagc actagaacca
    19141 atcgtagcaa gtgtattttc tttaacttga ccacctagca taattgtttt aatacctttt
    19201 ttaagtaaag cttctacatg tgttaaacca ttagttacca caatgatatc tttcgcttga
    19261 atatatttaa ttagctccaa tgtagatgaa ccagcatcga taaataagca ttcattatcg
    19321 ttgatttgat tagctgctat tttagcaatc attttctttt catcaagatt cgttgctaat
    19381 ttttcagtta aattcgcctc aaccatacga ttttctttta acattgcacc accatgcaca
    19441 cgttgcagtt tccctaattg ttgtagttta gataaatctc ttcgtattgt tgaagcactg
    19501 caaccagttc gatctattaa ttcttgtaaa gtcaaaaaat ctttgtgcga aagttcttct
    19561 aatattaact cgtgtctttt ttcagtaatt atcatcatca ccccatctca ctttgtatta
    19621 taatgagtac gctttcactt ttcaatcata aattaccaaa aacaatcaca aacactcaaa
    19681 ttgtgacgat aaaattacat ttttatgttc aaatactcaa ttataatgac tgtttatgat
    19741 cgtaaaatgc taattcaatc aaaaaggagt gtggcagaaa tgaattttat aaaaatttca
    19801 tctctgtcac actcctaatc ttgccccata ctttttgttg ttattaatca ttcatggata
    19861 actgctccaa ttttgccttt agttattgta atcaaatcct caacagctat tgtgatttgc
    19921 attgttcgaa gcccaccact cacactgata tgactataat tttcacacga tttgtcaacg
    19981 actgttggaa acaatgtttt cataccaaca ggcgaacacc caccacgaat gtatcccgtt
    20041 acatttttca aattatctaa aggcataagc tgcaatttct tctctccaac caaagcagct
    20101 gcctttttca tatctaaagt ttcacttact gggataacaa atacaaaatg ttcatgtttt
    20161 gtattttcta gaacaagcgt tttaaatact gttttaacat ttgcttgtat gagttctgcg
    20221 acttcttgac cagataaatg ttcttctggt acctcaaagc tatgaacttc gtatttaatt
    20281 tttgcacgat caagcatacg catcgcgttc gttttcttat ttttagccat ttcaacacct
    20341 catttatact tcttcagaac tatattaaca ttttaaaata cattacggat tggttgattt
    20401 tgacataaca tttacatcat tttataagtt gaattcacct ttttaaatta aattattgca
    20461 aaataaaaac agtatatcca attagaatac actgttccat ctattatttc gttattatgc
    20521 cttgatttga aatacttaca tgttgcttac tagttgaatt atcattggaa tacacataat
    20581 tatatccatg atttgtctta gaaattcttt ctaaaccata attatcattg atatgcttat
    20641 ctgcataacg atcagcaata acttggcact cttgttcgtt aaaagaattt gatttatttg
    20701 cattcacacg atgtttaaaa tgattttgct ttttcacatt ttcattcact tcagatgctt
    20761 tagcagtatg aatattgaga atatccattt tctttttata agtatcttca gttgatacat
    20821 ttatcgcgtt aagcgtatta attgttttat gtaccttagc aagcgcatct tcatttgttg
    20881 ctaatattaa taaaccaatt aaacaaacgg ttaatattac agcaacaaag cctaaaaata
    20941 aattttttac tgtcatcccc ttacccacat ttccttactc attttttatc atcttaacac
    21001 attaacgtta atgaattagg tatgtggatt gcaatttaaa aagaatgtaa tatttgtaac
    21061 atgtttatta ctcaaaatca ttttttgtta catataacat ttaaatataa agagtaaaac
    21121 atttagtcaa tcgaatcttt ttaaatgtaa agcatcgaat tttcttaata aaattcataa
    21181 gaaaaacgat gctaatcatt cataatatta cgctttactt tatatcaata tacaccaaaa
    21241 tacttatgct acatattttg ttctttcaat tttgctctat gttgcttttc aattaaaaca
    21301 ataacaacac ctgctaatga aacacctata gccatataaa ttggtgcttc aatgtgtaca
    21361 tcaaataacg cacctgcgat taaaggacct atgaaattac ccatactagt gaatgtcgag
    21421 ttcaatccgc ctgcaaagcc ttgcctttct ccagcaatat tagaaaaata atttgtaatg
    21481 gctggtcgta tcatatcaaa acctatgaag acaacaaaac tgattaacat tattgaccaa
    21541 tagtcattag caaaaactaa taatattaag acaacaactg aatataataa tgaccaagct
    21601 ataaatgtta actctgagaa atacttcata aatttatcga agaaatagat ttggaaaagt
    21661 gccccaaata taccgccacc cgtaatagca atcgaaatat ctttaggtga ataatttacc
    21721 ttgtcagctg tgtatagtga atacaatgtt tcaaatgcag ataaaccaaa cgataataca
    21781 agtgttaaaa taactggtgt aataaacact ttccagttaa ttttcgttag caattgtggc
    21841 tctaactttt ggaaaccact tgtcgtagac tttttcggat cgtgaatcaa tacaattgac
    21901 attataaatg ctagaatacc taatgctcct gcaaagtaaa atggcatacg atgtgaaact
    21961 tctgccataa atccaccaat ccctggtcct aaaatgaatc cagaattgat aatcgctgac
    22021 atgtagccaa agttttttgc tttttgatgg cttggtgaaa tgtcagctat taaacctgtc
    22081 acaccaggca ttaccatacc agcactcata ccaccaatca ctctcgataa catcaatacc
    22141 gaaaaattgt ggccaactgc aaacataaat tctgacactg aaaacaaaat taatcctata
    22201 catataatta atttcttccc taatttgtca gctagcgtac caccaaacgg cgatataatc
    22261 atttgagata acgcaaaagc agcaactagt aatcctaaat cactaccagt taatcccaaa
    22321 tcttttaaat aaacaggcaa gactggtatt actaaaccga tacctaaaaa aatcaagaaa
    22381 atattaaaat ataagacaaa aatctgttta ttcatatgct cacctctttt ttctccatgt
    22441 catgcttaaa gctgttattc ttcttttcca cgacagattg caattcatgc taaatattcg
    22501 taaaaagttt ctatattgta ttacaacatt gctacatttg acaatatttt ttctttgtaa
    22561 ttatcactat tttccattta attgtataat taatgacata ttaaaataaa ggggattgat
    22621 catatgaaat tgcaaaaaag taatcatact atactactag tgttggaaaa aggagaagat
    22681 atcgttgaat gtataacaac ctttgcagat gaccaagact taacatttac atctgtcagc
    22741 ggcattggtg catgtgatga cgttgtatta aaatttttca atttaacaac gaaacaatac
    22801 gaagaaaaac atattactga gccactggaa ttgacgagct tattaggtaa tatttctcgt
    22861 ctagacaatg gtcattttgc acatttgcat gctactttcg gtactcaatc atatgaaact
    22921 tttagcggac atctcgctaa agcaattgtt tctgcaactg ctgaaatcat tcttactgta
    22981 actgatttgg acattcaacg ttcattcaaa gatgcagtcg gtttaaattt acttgatcct
    23041 caatagcact ttacttttta gttgaatcaa attttattac aagttatcat aagtgcttaa
    23101 tcataaatgc attctcgttt attaatatac ttcaacgagt taaattaaat ttttaattag
    23161 gaattgaatg cgtttataac tatcacattt gaatgaataa ctttttcatt agaaagtagt
    23221 gtttattatt gatgcaatct cgatacatcg ctagaattta ctttttaatt ttatttatcg
    23281 tctccctttt tgagactaaa atttttcaat ttatgacttt aaatttgttt ttagcatata
    23341 taccttttga gcttagtcta ttattacgat tattcaaacc gaataaaaag tatgaatggc
    23401 cactctttat catatatagt tttatatttg tgttactttt accaaataca ttttatatgg
    23461 taactgattt aatccatttg aaccaatttc aattcaactt ttacgcagga ctaaatttaa
    23521 gtgagtggaa atactttacc tatttactgc ttggtgtctt cttagcggtt tatgtgatga
    23581 ttttaatcta ttttgaaata ctaacattga cttctcaccg ttggcttaat agattaattg
    23641 ttgtcgtgct aatgttttta aatggattcg gaatttatat tggtcgtttc ttacgcttac
    23701 attccgttta tttctttaat gagcccttac atgttctaaa tcacattcta gaaagtctaa
    23761 cgcttaaaac tgctatgttt gtatgtttta tggttgtcat gcaagcagca attctattat
    23821 ttggaaaagg agtgcgtttg aacaagtgac aataaatata atcgttttaa tagtatctat
    23881 cattgtcttt caattgatta ttggacacat atggcatgat attggtttaa gttatttaag
    23941 aagtatctta ctcatgatgc tgccatttgg actaggtgta tttatacaac aagtgtcgta
    24001 ttacgaacga caatatccta aatggcaagt gcctcaaaac ataaaagtta gattgaaata
    24061 tatttatctc gctacgtttt tagagtatgt cgtactatac ctcactttat ttacggacat
    24121 actaagataa catcaaatta ataagaaaac taaatacaac ttatatattt gaggaaagcc
    24181 aatttctatc acatcaataa tagaaattgg cttatttata ttgcttaaga actttatatt
    24241 ctgctccaga ttttactcat gccagatcta tcaattgagc cgaattagtc tagcgcttta
    24301 aatgtagcta aaacttgtgt acgtttttct tgatgatcta ccatttgtct tggataatga
    24361 ctacctaatt caatcccctg ttcaaaaagg ttggactcat tgcgttgtgt atcatgcaaa
    24421 tattttgcat caatttgatt aaaaatcgga agatatgttt tgatatacaa agctttagca
    24481 tcaaagcgtt cactctgtct tattggatta aacattctaa aatacggcac tgcatccgta
    24541 cctgtagaag cagaccattg ccatccatga atatttgatg ctgcatcata gtcaataagg
    24601 tgctttctaa agaatttttc tccccatgtc caatctataa ataaatcttt ggttaaaaat
    24661 tgcgacacaa ccattctcat tcgattatgc ataaaaccag tttgtgtcaa ttccattatt
    24721 gctgcatcaa tgattggaaa tcctgtttgc ccttcgcacc atgcattaaa atccgcttca
    24781 ttttgcgacc attttatctg tcgatattta ggtttgaaag cttggtatga ggtttcagga
    24841 tactgtgtca ttaacacata ataaaattct ctaaaaatga gttcacgtat aaatgcttca
    24901 tagtttgcct catcttcatc ataacgcgcc aataaatcat taaaaatttc aataatatct
    24961 aataatccat atgctaaagc aacacttagc tgacttgtta atacttcagg taaatagtct
    25021 cttccgcttt cgtaattctg tatatcttga tctaaaaaat gttgccattt attctgttca
    25081 attatttgtg aggatccaaa tgctatgtca tcaaacacta aatcatcagg tgatgcaatg
    25141 acaaagtctt cgaatgattt taaatcatag tgatatacgt ctctatgcct caagtaaggt
    25201 cgccattttt tatagaaact agtaaaaaca ttgtatggct ccccttgttg attcattgtt
    25261 ttactcgctt taaagtaatg attcccacgt atcaaagtaa cggcaatgcg tgcttcattg
    25321 aataaagaac gttgatgtaa aatatcatat tcttcaagat gataactcat aatatcaccc
    25381 gcagtcacta cttcagagat attgttttct ttacaaaatg aaatgatatc gtcataagac
    25441 ataacataag gtgtaagctt ttctgcgtgc aacgattggt aaaagcgctg caaagtaccg
    25501 cgataaaact gtgctttcaa atcagaagct gaagacatgt cctcttccgg aatgataaaa
    25561 taacatgcat caatgtcttc tttttgttga taaatatatt caaataatgg attgtgttcc
    25621 attcgaaaca ttcgatttaa taacacagca attgccataa acaaaacacc cttcaattat
    25681 ctttttattt tattaagctt tttgtcactt
//
LOCUS       DALAGF010000029        25637 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.029, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000029 DALAGF010000000
VERSION     DALAGF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25637
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.029"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            43..840
                     /locus_tag="O2692_001127"
     CDS             43..840
                     /locus_tag="O2692_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCZ4298763.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            980..2740
                     /locus_tag="O2692_001128"
     CDS             980..2740
                     /locus_tag="O2692_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HCZ4298764.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIYGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSDGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            2741..3607
                     /locus_tag="O2692_001129"
     CDS             2741..3607
                     /locus_tag="O2692_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HCZ4298765.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            3701..3994
                     /locus_tag="O2692_001130"
     CDS             3701..3994
                     /locus_tag="O2692_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HCZ4298766.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            4128..5672
                     /gene="miaB"
                     /locus_tag="O2692_001131"
     CDS             4128..5672
                     /gene="miaB"
                     /locus_tag="O2692_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCZ4298767.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFIKEVE
                     PEMVIQ"
     gene            5673..6038
                     /locus_tag="O2692_001132"
     CDS             5673..6038
                     /locus_tag="O2692_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HCZ4298768.1"
                     /translation="MYNKDDVLNQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            6065..6556
                     /gene="thiW"
                     /locus_tag="O2692_001133"
     CDS             6065..6556
                     /gene="thiW"
                     /locus_tag="O2692_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HCZ4298769.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
     gene            6859..9477
                     /gene="mutS"
                     /locus_tag="O2692_001134"
     CDS             6859..9477
                     /gene="mutS"
                     /locus_tag="O2692_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCZ4298770.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            9490..11499
                     /gene="mutL"
                     /locus_tag="O2692_001135"
     CDS             9490..11499
                     /gene="mutL"
                     /locus_tag="O2692_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCZ4298771.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNIHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMEENQNDEIVIK
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDGTTVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            11505..12047
                     /locus_tag="O2692_001136"
     CDS             11505..12047
                     /locus_tag="O2692_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCZ4298772.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
     gene            12512..13330
                     /locus_tag="O2692_001137"
     CDS             12512..13330
                     /locus_tag="O2692_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCZ4298773.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
     gene            13459..14955
                     /gene="glpK"
                     /locus_tag="O2692_001138"
     CDS             13459..14955
                     /gene="glpK"
                     /locus_tag="O2692_001138"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCZ4298774.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKA
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            15113..16786
                     /locus_tag="O2692_001139"
     CDS             15113..16786
                     /locus_tag="O2692_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HCZ4298775.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            16936..17850
                     /locus_tag="O2692_001140"
     CDS             16936..17850
                     /locus_tag="O2692_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4298776.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            17868..18803
                     /gene="miaA"
                     /locus_tag="O2692_001141"
     CDS             17868..18803
                     /gene="miaA"
                     /locus_tag="O2692_001141"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCZ4298777.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            18818..19051
                     /gene="hfq"
                     /locus_tag="O2692_001142"
     CDS             18818..19051
                     /gene="hfq"
                     /locus_tag="O2692_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCZ4298778.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
     gene            complement(19273..19749)
                     /locus_tag="O2692_001143"
     CDS             complement(19273..19749)
                     /locus_tag="O2692_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCZ4298779.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            19861..21099
                     /gene="hflX"
                     /locus_tag="O2692_001144"
     CDS             19861..21099
                     /gene="hflX"
                     /locus_tag="O2692_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCZ4298780.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            21118..22356
                     /locus_tag="O2692_001145"
     CDS             21118..22356
                     /locus_tag="O2692_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCZ4298781.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEKVITRLKNVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFETKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
     gene            22599..22967
                     /locus_tag="O2692_001146"
     CDS             22599..22967
                     /locus_tag="O2692_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCZ4298782.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            22986..24326
                     /gene="glnA"
                     /locus_tag="O2692_001147"
     CDS             22986..24326
                     /gene="glnA"
                     /locus_tag="O2692_001147"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCZ4298783.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            24819..25016
                     /locus_tag="O2692_001148"
     CDS             24819..25016
                     /locus_tag="O2692_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298784.1"
                     /translation="MNCYDEIFNTIKELIENKEISSYQINKDTGISYGNINAMRRRER
                     RIENLSLKNAKILYEYAKKVL"
BASE COUNT         9520 a         3486 c         4866 g         7765 t
ORIGIN      
        1 acgtatgaat taaactagta tttatgagag gatgaacaaa acatgagaat aatgtttata
       61 ggggatatcg taggtaaaat tggacgagac gcaattgaaa cgtacatacc tcaactgaag
      121 caaaagtata aaccaacagt tacaattgta aatgctgaaa atgcagcaca tggtaaaggt
      181 ttgactgaaa aaatatataa acaattacta agaaatggtg tagatttcat gactatgggt
      241 aatcacacat atggtcaacg tgaaatttat gattttatag atgaagcaaa acgactagta
      301 agaccagcga attttccgga tgaagcgccg ggaattggta tgagatttat acaaattaat
      361 gatattaaac ttgcagttat taatctgcaa ggaagagcgt ttatgccaga tattgatgat
      421 ccttttaaaa aggcagatca attagtcaag gaagcacaag aacaaactcc gtttatattt
      481 gttgattttc atgcagaaac aacttctgaa aagtatgcaa tgggatggca tttagatggt
      541 agagctagcg ctgttgttgg aacgcataca cacattcaaa cagcagatga acgtatttta
      601 ccaaagggga cagggtatat aacggatgtt ggtatgacag gtttttatga tggcatttta
      661 ggaataaata aaacagaggt aattgagcgt tttatcacta gtttgccaca aagacatgtt
      721 gttccaaatg aaggtagaag tgtattatct ggtgttgtta ttgatttaga caaagaaggt
      781 aaaacaaagc acatcgaacg tatattgata aatgatgacc atccattttc aacattttaa
      841 aattacgtaa gtaaacattc gaattggacc ctatcgtcca ttagtatgaa tttaatatag
      901 taccactgtt tacatagtaa atcggtggtt ctttttgtta tcatttaata tgaaatatat
      961 ccataggagg catataacta tgaaaccaca attatcgtgg aaagttggcg gtcaacaagg
     1021 cgaaggtatt gaatcaactg gggaaatctt cgctacggct atgaatagaa aaggatatta
     1081 tttatatgga tatagacatt tttcaagtcg tatcaaaggt ggacatacga ataataaaat
     1141 tagagtttct acgacgcctg ttcatgcaat tagtgatgat ttagatattt tgattgcatt
     1201 tgaccaagaa acaattgatg ttaaccatca tgaaatgaga gaagacagta ttattttagc
     1261 tgatgccaag gctaaacctg tgaaaccaga aggatgtcat gcacagctta ttgaattacc
     1321 ttttacagca accgctaaag aattaggtac agcattaatg aaaaacatgg ttgcaatagg
     1381 tgctactagc gcattgatga atttgaatac aaatacattt gaagaactta ttactaatat
     1441 gttttctaaa aaaggtgaca aggtagttga agtcaatatc caagcattaa acgaaggtta
     1501 tcaattaatg caatctcgct tacctgaaat ctacggggac tttgaattag agtcaacaga
     1561 tgcactacca catctatata tgattggtaa cgatgccatt ggattaggtg caattgctgc
     1621 aggttcacaa tttatggcgg catatcctat tacacctgcg tctgaagtta tggaatatat
     1681 gattgccaat atatctaaag taaacggagc ggttattcaa acagaagatg aaattgctgc
     1741 tgtaactatg gctattggtg caaattatgg tggagtcaga gcgtttacgg ctagtgctgg
     1801 tccaggttta tctttaatga tggaagcaat tggattatct ggtatgactg aaacgccatt
     1861 agtcattatt aatacccaac gaggtggacc ttctactgga ttacctacga aacaagaaca
     1921 gtcagattta atgcaaatga tttatggtac acatggtgat attccaaaaa ttgttgtagc
     1981 accaacagat gcagaagatg cattttattt aactatggaa gcatttaatt tagcagaaca
     2041 atatcaatgc cctgttatag ttctaagtga tttgcaatta tctttaggta aacaaactgt
     2101 tgaaaaatta gattataatc gtattgaaat taaacgtggt gaaatcattc aatctgatat
     2161 tgaacgtgaa gaagatgata aaggttattt caagcgttat gcgttaacat ccgatggtgt
     2221 ttctcctaga cctatccccg gtgttaaagg aggtattcat catataactg gtgtggaaca
     2281 caatgaagaa ggtaaaccta gtgaatctgc gtcaaataga caacaacaaa tggaaaaacg
     2341 aatgcgtaaa attgagcagt tactaattga atcgccagta gaagctaact tacaacatga
     2401 ggatgcagat attctttata tcggttttat ttctacaaaa ggtgcaattc aagaaggtag
     2461 taaccgtttg aatcaacaag gcataaaagt taacactata caaattagac aattgcatcc
     2521 attcccaaca agcgttattc aagatgcagt taataaagcg aagaaagtcg ttgtagtgga
     2581 gcacaattat caaggacaat tggctagtat tataaaaatg aatgtcaata ttcatgataa
     2641 gattgaaaat tatacaaagt atgatgggac acctttccta ccacatgaaa tcgaagaaaa
     2701 aggcaaaata attgctactg aaataaagga gatggtatag atggcgacat ttaaagattt
     2761 tagaaataat gttaagccta actggtgccc cggatgtggc gatttctcag tacaagctgc
     2821 aattcaaaaa gcagccgcaa atatagggtt agaacctgaa gaagtagcta tcatcaccgg
     2881 tataggatgt tctggccgtc tttcaggata tattaattct tatggcgttc attctattca
     2941 cggacgtgca ttacctttag ctcaaggtgt aaaaatggcg aataaagatt taactgttat
     3001 tgcatcggga ggagatggtg atggttatgc tataggtatg gggcatacaa tccatgcttt
     3061 aagaagaaat atgaacatga cgtatatagt catggataat caaatttatg gtttgacaaa
     3121 gggacaaaca tcgccgtcat cagcagtagg atttgttact aaaacaacgc caaaaggtaa
     3181 tatagaaaaa aatgttgcgc ctttagaatt agcattatca tctggtgcca catttgtagc
     3241 ccaaggtttt tcaagcgata ttaaaggatt aacaaaacta attgaagatg caattaatca
     3301 tgatggattt tcattcgtta atgtcttttc accatgtgtg acttataata aaattaacac
     3361 atacgattgg tttaaagaac atttaacaag tgttgatgac attgaaaatt atgattctac
     3421 agataaacaa ttagcgacta aaactgttat tgaacatgaa tctttagtaa ctggtattgt
     3481 ttatcaagat aaagaaacac catcatatga atctcaaatt aaagagttag atgatacacc
     3541 acttgctaaa agagatatca aaattactga agacaccttc aatgcattaa ctgaacaatt
     3601 tatttaataa ttaaaactga gtcgtataaa tgtatttata acagatccat ttatgctact
     3661 cagtttttta ctattacaaa aaataaagga gtttttaaaa atgaaagaca cattaatgag
     3721 tatacaaata attcctaaaa caccaaacaa tgacaatgtt ataccttacg tagacgaggc
     3781 gattaaaata attgacgaat ctggtttgca ttttagagta ggtccgttag aaacgacagt
     3841 acaaggaaat atgaatgaat gtttaatttt aatacaatca ttaaatgaac gaatggtgga
     3901 acttgaatgt ccaagtatta ttagccaagt taagttttat catgtgccag atggcatcac
     3961 tattgaaact ttaactgaaa aatatgatga ataacattaa aagtgaagta aactggattt
     4021 gaattggctt gttagagatg acgtataact ttaactgttt ttgcacttta tagttaaatt
     4081 taatataatt attaaatgat acgggcaaat agaaaggatt ttgtaaagtg aacgaagaac
     4141 aaagaaaagc aagttctgta gatgttttag ctgagagaga taagaaagca gaaaaagatt
     4201 atagtaaata ttttgaacat gtttatcagc cgcctaattt aaaagaagcg aaaaaaagag
     4261 gtaaacaaga agttcgttat aatagagatt tccaaattga tgaaaaatat cgcggtatgg
     4321 ggaacgagcg tacattttta attaaaacat atggatgtca aatgaatgca catgacactg
     4381 aggtcattgc tggtatactt gaagcattag gctatcaagc aacgactgat attaacactg
     4441 cagatgttat tttaattaat acatgtgcga ttagagaaaa tgccgagaac aaagtgttta
     4501 gtgaaatagg taatttgaag catttgaaaa aagaacgacc tgatatttta atcggtgttt
     4561 gtggttgtat gtcacaagaa gagtcagtag tgaataaaat tttaaaatcg tatcaaaatg
     4621 tagatatgat atttggtaca cataatattc atcatttacc agaaatttta gaagaagcat
     4681 acttatctaa agcaatggtt gttgaagtat ggtctaaaga aggagacgtt attgaaaatc
     4741 ttccaaaagt ccgtgaaggc aacattaaag catgggtcaa tattatgtat ggttgtgata
     4801 agttttgtac atattgtatt gttccattta caagaggtaa agaacgaagc cgtagacctg
     4861 aagacattat agatgaagta cgtgaacttg ctcgtgaagg ttacaaagaa ataacgcttt
     4921 taggtcaaaa tgtaaattct tatggtaaag atttacagga tatagaatat gacttaggag
     4981 atcttttaca agcaatttct aaaatagcga ttccaagagt tcgtttcaca acaagtcatc
     5041 cttgggactt tacagatcac atgattgatg ttatttcaga gggtggtaat atcgttccgc
     5101 atatccactt gccagttcaa tctggaaata atgcagtatt aaaaataatg ggtagaaaat
     5161 atacacgaga aagttatttg gatttagtaa aacgaatcaa agatagaatt cctaatgtag
     5221 cattaactac agatattatt gtagggtatc caaatgaatc agaggaacaa tttgaagaaa
     5281 ctttaactct gtatgatgaa gttggttttg aacatgcata tacgtacttg tattcacaac
     5341 gtgatggtac gcctgctgct aaaatgaaag ataatgtacc tttaaatgtc aaaaaggaac
     5401 gattgcaacg tttgaataaa aaagttggtc attattcaca aatagctatg agtaagtacg
     5461 aaggacaaac tgtaacagta ctttgtgaag gaagtagtaa aaaagatgat caggttcttg
     5521 ctggctacac tgataaaaat aagctagtta atttcaaagc gcctaaagaa atgattggta
     5581 aactagtgga agtacgaata gatgaagcta aacagtattc attaaatggc agttttataa
     5641 aggaagtaga gccggaaatg gtgattcaat aaatgtataa taaagatgac gtgttgaacc
     5701 aagcggataa tattgcaaat aaaattaaaa atttggatac tatcaaaaca tatcaacaaa
     5761 ttgaagcaca gattcatcag aaccaaacga taaagactaa aatggatatg ttaaaaaagc
     5821 atcaaaaaca agcagtaaac tttcaaaatt acgggaaaca aaatgcgcta gaacagtcgg
     5881 aacataccat tcagagtata gaagcagaaa taaatacatt gcccatagtt gaacagtttc
     5941 aaacttcaca atatgaagcg aatcaattat tgaaaatgtt tgtatcaaca atggaaacac
     6001 gtttaaatga ccataataaa gccaagcata gtgattaatt acaaacaaaa aggagaacaa
     6061 tcatatgaaa tcaagaaaac tggctataac tgcactttta attgcaataa atgttgtatt
     6121 aagcagtatt atcatcattc ctctaggacc agttaaggca gcaccagtac agcattttgt
     6181 aaatgtatta agtgcggtca tagtaggtcc ttggtatgga ttagctcaag cgcttatatc
     6241 atcaatttta agagttcttt ttggtactgg tacagctttt gcatttccgg gtagtatgat
     6301 tggagtttta ttggctagta tgttttacat atatcgtaaa catatattta tggccgcggt
     6361 cggtgaagta cttggaactg gtgtcatcgg aagtttaatt tgtataccat tagcatattt
     6421 ccttgggctt caagacttct tcattaaacc gttaatgatt acgttcatag tctcaagtgc
     6481 tatcggatct attataagtt atttcttatt aattactcta aaaaaacgtg gtattcttca
     6541 aaggtttata aaataatttt atgatatatg atttcaaaca gagtcattgt tcattttaat
     6601 attgaacgcc cttacttaat taaatgtctt gaatgttaga attttaattt aattctaaac
     6661 atgctattga cacattttat taggtttgag gcgttttctt atacatttaa gatgcttcgt
     6721 gattatatgg tatgaatagg tttgtaaaag taagtatgtt aaaatgtata agttaaataa
     6781 ttaataatta aaaagcttac aaacgaatat attgtaaaga caaattttaa aaagagacaa
     6841 gggttgtgaa gaaataatat gtctaatgtt acaccaatga tgcagcaata tttaaaaata
     6901 aaatcagaat accaagattg cttattattt tttagactag gtgatttcta tgaaatgttt
     6961 tatgaagatg ccaaggaggc atcacgtgta cttgaaatta ctttaactaa aagagatgct
     7021 aaaaaagaaa atccaattcc gatgtgtggt gttccgtatc attctgcaga tagttatata
     7081 gatacacttg ttaataatgg atataaagta gctatttgtg aacagatgga agatccgaaa
     7141 caaacgaaag gtatggttag acgtgaggta gtaagaattg tgactccagg aactgtgatg
     7201 gagcaaggtg gtgtagatga taaacaaaat aactatattt taagttttgt tatgaatcaa
     7261 cctgaaattg cgcttagtta ctgtgatgtt tctactggcg aattaaaggt tacacatttt
     7321 aatgatgaag cgactttatt aaatgaaatt acgacgataa accctaacga agttgttatc
     7381 aatgacaata tttccgataa tttaaaaaga caaattaata tggtgacaga aacaataaca
     7441 gtcagggaaa cgttatcatc agaaatctat agtgtgaatc aaactgaaca taaattaatg
     7501 tatcaagcga cacaattatt gctagattat attcatcata cacaaaaacg tgatttatcg
     7561 catatcgagg atgttgttca atatgcagct atagattata tgaaaatgga tttttatgct
     7621 aagagaaacc ttgagttaac ggaaagcatt cgattaaaat caaaaaaagg aacgctactt
     7681 tggctaatgg acgaaacgaa aacaccaatg ggagcacgcc gcttaaaaca atggatagat
     7741 agaccactaa taagtaaaga acaaattgaa gcacgattag atatcgttga tgaatttagt
     7801 gctcatttca tagaaagaga caccttaaga acatatctta atcaagtgta tgatattgaa
     7861 cgtcttgttg ggcgtgttag ttacggaaat gttaatgcga gagatttaat tcaacttaaa
     7921 cattccattt ctgaaatacc gaatattaaa gcattactaa attctatgaa tcagaatact
     7981 cttgtacaag ttaatcaact agaacccctt gatgatttac ttgatatatt agaacagagt
     8041 ttagtagaag aaccaccaat ttcagttaaa gatggcggac tattcaaagt tggttttaat
     8101 acgcaattag atgaatatct tgaagcttca aaaaacggaa aaacatggtt agcagaatta
     8161 caagccaaag aaagacaacg tacaggaata aaatcattga aaataagctt taataaagtg
     8221 tttggttatt ttatagaaat aacacgtgcc aacttgcaaa attttgaacc aagtgaattt
     8281 ggttatatga ggaagcaaac gttatcgaat gctgaacgtt ttataactga tgaacttaaa
     8341 gaaaaagaag atatcatttt aggtgcggaa gacaaagcca tcgaattaga atatcaatta
     8401 tttgttcagc tacgtgaaga agttaaaaaa tatactgaac gtttacaaca acaagctaaa
     8461 attatttcag agctagattg tttacagagc tttgcagaaa ttgctcaaaa atataattac
     8521 actaggcctt catttagtga aaataaaaca ttagaattag tggaatctag gcacccagta
     8581 gtggaaagag taatggatta taatgactat gtgcctaata attgtcgatt agataatgaa
     8641 acatttatat atttaattac aggtccgaat atgtctggta aatcgacata tatgagacaa
     8701 gttgccataa ttagtataat ggcccaaatg ggagcttatg tcccttgtaa agaggcagtg
     8761 ttacctatat ttgatcaaat attcactaga ataggtgcgg cagatgattt ggtttcaggt
     8821 aagagtacgt ttatggtaga aatgctagaa gcacaaaagg cattaactta tgcaacagag
     8881 gatagtttga ttattttcga tgaaattgga cgtggtactt caacgtatga cggtttagct
     8941 ttagcgcagg caatgataga gtatgtagct gaaacatcac atgctaaaac gttattttca
     9001 acacattatc atgaattgac aacattagat caagcattac caagtctaaa aaatgttcac
     9061 gtcgctgcta atgaatataa aggtgaactt atattcttgc ataaagtcaa agatggtgca
     9121 gttgacgata gttatggtat tcaagttgcg aaattagctg atttacctga aaaagttatt
     9181 agcagagcac aagtgattct aagcgagttt gaagcgtctg ctggtaaaaa atcatcgata
     9241 tcaaatttaa aaatggtcga aaatgaacct gaaattaatc aagaaaattt aaacttaagt
     9301 gttgaagaaa caactgatac tttatctcaa aaagactttg aacaagcatc atttgatttg
     9361 tttgaaaatg atcaagaaag cgagattgaa ctacaaatta aaaatttgaa tttatctaat
     9421 atgacaccaa ttgaggcatt ggtgaagtta agtgaattac aaaatcaatt aaaatagagg
     9481 tgttgcaaaa tggggaaaat taaagaactc caaacctcat tagcaaataa aatcgcagca
     9541 ggtgaagtag ttgaaagacc gagttctgtt gtgaaagaac tgttggaaaa tgctatagat
     9601 gcaggcgcta cagaaataag cattgaagta gaggaatctg gcgtccaatc tattcgcgta
     9661 gtcgataatg gaagcggaat tgaagcggaa gacttaggat tagtatttca tagacatgcg
     9721 actagtaaat tagatcaaga tgaagattta tttcatatta ggacattagg attccgtggt
     9781 gaagcactag ccagtatttc atcagttgct aaagtaacat tgaagacttg cacggataat
     9841 gctaatggaa atgaaatata tgtagaaaat ggtgaaatat taaatcataa gcctgcaaaa
     9901 gcgaaaaaag gaacagatat acttgtagaa tcattatttt ataatacacc agcacgttta
     9961 aaatatatta aaagtttata cactgaacta ggtaaaataa cagatattgt caacagaatg
    10021 gcaatgagcc atccggacat tcgaatagca ctcatttcag atggcaaaac aatgttaagt
    10081 acaaatggtt caggacgaac taatgaagtg atggcagaga tttatgggat gaaagttgca
    10141 cgagatttag tacatatatc tggagataca agtgattatc acattgaagg ttttgttgca
    10201 aagcctgaac attctagaag taataagcac tatatttcta tttttattaa tggacgatac
    10261 attaaaaact ttatgctaaa taaagcgatt ttagaaggct atcatacact cttaacaata
    10321 ggtaggttcc cgatttgtta tattaatatt gaaatggatc caatcttagt agacgtaaat
    10381 attcatccaa caaaactaga agtgcgttta tcaaaagaag agcaactata ccaattgatt
    10441 gtgagcaaaa tacaagaagc atttaaagac cgtatattaa ttcctaaaaa taacttggat
    10501 tatgtgccaa aaaaaaataa agtattacat tcattcgaac aacaaaaaat cgaatttgaa
    10561 caaagacaaa acacagagaa taaccaagag aagacgtttt catctgaaga aagtaacagt
    10621 aagccattta tggaagaaaa tcaaaacgat gagatagtta taaaagaaga ttcatataat
    10681 ccattcgtaa cgaaaacatc tgaaagttta atagctgatg atgaatcttc tggttataat
    10741 aatacacgtg aaaaagatga agactacttc aaaaaacaac aagaaatttt acaagaaatg
    10801 gatcaaacat ttgattcaaa tgatggtaca actgtgcaaa attatgagaa taaagcgtct
    10861 gatgattatt atgatgtaaa cgatattaaa ggaacaaaaa gtaaagaccc taaacgaaga
    10921 attccatata tggaaattgt tggacaagta catggaacgt atattattgc tcaaaatgaa
    10981 tttggcatgt acatgattga ccagcatgca gctcaagaaa gaataaaata tgaatatttt
    11041 cgagataaaa taggtgaggt taccaatgaa gtacaagatt tattaatccc gttaacattt
    11101 catttttcaa aagatgaaca attagtcatt gatcaatata aaaatgagct tcaacaagta
    11161 ggtatcatgt tagaacattt tggtggtcat gattatattg taagtagcta tccagtttgg
    11221 ttccctaaag atgaagtaga agaaattatt aaagatatga ttgagctaat tttggaagag
    11281 aaaaaagtag atatcaaaaa attacgtgaa gatgtagcaa tcatgatgtc atgtaaaaaa
    11341 tccattaaag cgaatcatta tttacaaaaa catgaaatgt ctgatttaat tgatcaatta
    11401 agagaagcgg aagatccatt tacatgtcca catggtcgtc caataatcat taatttttca
    11461 aaatacgaat tagaaaaatt atttaagcgt gtgatgtaga gaggatgaat caagtgaata
    11521 acaacatatt gcctgctata agaaacatta aagatttaga gaaactgatt aaaacagact
    11581 ataaaatgtg tgtgcttcta gatatgcata taggacatat aaaaagtatt atggaattgc
    11641 tgaagcaaaa tcatatagag tgttttattc atatagattt gataaaaggt ttaagccacg
    11701 atgaatttgc aagtgaattt attattcagc aatacaagcc aaaaggtatc gtatcgacta
    11761 aatctaaagt aataaaaaaa gctaaatcat taaatacttt aacgattttt agagtattta
    11821 ttattgatag tcaagcattg aaacgcagta tagatttgat aaaaaaagtt gaacctgatt
    11881 ttgttgaagt acttccaggt gttgcgagta aagcgattca tcatattcag aaagaaacaa
    11941 acacacaagt cattgcaggt ggcctaatta atacaataga tgaagtcaat gaagctgtta
    12001 aaaatggagc gaaatatgta actactagtt atgataaact ttggtaaatc ataagtctgc
    12061 aaaaatttca tttttcacga gatttagtca tataacataa taaaaaagcc gttttcatat
    12121 gggatatgca gtaggtatca aacaaaaaat aatcaaataa aataattagc acctctaaaa
    12181 cgattaaaat cattaaagtt ttagaggtgt ttattagtaa aaaatgcaac tagtcataaa
    12241 aaatatggct gaagaatgtc aattaaacaa aagagcgtca taaataaatt agttaggatt
    12301 ggtatttcta gttttcaaat taaactactt ttataaagac ttgaaaaatt aaatttgaca
    12361 aaatttttac aaaaaccatt gacaacgctt tcatatcgat agtatcataa caaatataat
    12421 aaaagttaat acatagaata gagacgggag atttctacga gccaaactgc tagtgtagga
    12481 atctctttgt ctttttggga ggacatttaa tatgaatgta tatttagcag aattcctagg
    12541 aactgcaatc ttaatccttt ttggtggtgg cgtttgtgcc aatgtcaatt taaagagaag
    12601 tgctgcgaat ggtgctgatt ggattgtcat cacagctgga tggggattag cggttacaat
    12661 gggtgtgttt gctgtcggtc aattctcagg tgcacattta aacccagcgg tgtctttagc
    12721 tcttgcatta gacggaagtt ttgattggtc attagttcct ggttatattg ttgctcaaat
    12781 gttaggtgca attgtcggag caacaattgt atggttaatg tacttgccac attggaaagc
    12841 gacagaagaa gctggcgcga aattaggtgt tttctctaca gcaccggcta ttaagaatta
    12901 ctttgccaac tttttaagtg agattatcgg aacaatggca ttaactttag gtattttatt
    12961 tatcggtgta aacaaaattg ccgatggttt aaatccttta attgtcggag cattaattgt
    13021 tgcaatcgga ttaagtttag gcggtgctac tggttatgca atcaacccag cacgtgattt
    13081 aggtccgaga attgcacatg cgattttacc aatagctggt aaaggtggtt caaattggtc
    13141 atatgcaatc gttcctatct taggaccaat tgccggtggt ttattaggtg cagtggtata
    13201 cgctgtattt tataaacata catttaatat tggttgtgca attgcaattg ttgtagttat
    13261 tattactttg attttaggtt acattttaaa taaatcatca aaaaaaggtg atatcgaatc
    13321 aatttactaa aataaaaaga aacgtaaata gcataattta acatgtttga ttcatggatt
    13381 atgctatttt ttcgccaaaa tttaacagat tttgtacaat gggttagcga ttatttttta
    13441 ataaaggaga tactactaat ggaaaaatat attttatcta tagaccaagg aacaacaagc
    13501 tcaagagcga ttttattcaa tcaaaaaggg gaaattgcag gggtagcaca acgtgagttt
    13561 aagcaatatt ttccacaatc aggttgggtt gaacatgatg caaatgaaat ttggacatct
    13621 gtgttagctg taatgacgga agtaattaat gaaaatgatg ttagagctga tcaaattgca
    13681 ggtatcggta ttacaaacca acgtgaaaca acggttgttt gggacaaaca tactggccgc
    13741 ccaatttatc acgcaattgt ttggcaatca cgtcaaacac aatcaatttg ttcagaatta
    13801 aaacaacaag gatatgaaca aacatttaga gataagacag gattactttt agatccgtat
    13861 tttgcaggta caaaagttaa atggattcta gacaatgttg aaggtgcacg agaaaaagca
    13921 gaaaatggcg atctattatt tggaacgatt gatacttggt tagtatggaa attatcagga
    13981 aaagctgcgc atattactga ttattcaaat gcgagtcgta cattaatgtt taatatccat
    14041 gatttagaat gggacgatga gttattagaa ctacttacag tacctaaaaa tatgttgcca
    14101 gaagttaaag cttcgagtga agtatatggt aagacaattg attaccactt ctatggtcaa
    14161 gaagtaccaa tcgctggagt agctggtgat caacaagcag cattatttgg acaagcttgc
    14221 ttcgaacgtg gtgacgtgaa aaacacatat ggaactggtg gcttcatgtt aatgaataca
    14281 ggtgacaaag cggttaaatc tgaaagtggt ttattaacaa caattgctta tggtattgat
    14341 ggaaaagtaa attatgcgct tgaaggttcc atctttgttt cgggttcagc aatccaatgg
    14401 ttacgtgatg gattaagaat gattaattca gcaccacaat cagaaagtta tgcgacacga
    14461 gttgactcta ctgagggtgt ttatgttgtt ccagcttttg taggtttagg aacaccatat
    14521 tgggattctg aagcacgtgg tgcgattttc ggtttaacac gtggaactga aaaagagcac
    14581 tttatccgtg caactttaga atcactatgt taccaaactc gtgacgttat ggaagcaatg
    14641 tcaaaagact ctggtattga tgtccaaagt ttacgtgtcg atggtggtgc agttaaaaat
    14701 aactttatta tgcagttcca agcagacatt gttaatactt ctgttgaaag acctgaaatt
    14761 caagaaacta cagctttagg tgctgcattt ttagcaggtt tagcagttgg attctgggag
    14821 agtaaagatg atatcgctaa aaactggaaa ttagaagaaa aattcgatcc gaaaatggat
    14881 gaaggcgaaa gagaaaaatt atatagaggt tggaaaaaag ctgttgaagc aacacaagtt
    14941 tttaaaacag aataaacttg tagattagac ttttgtataa acattgtgat acaatcaatt
    15001 taagttaata tttgaatcga gaagcgagag atttgttcga acatgtacaa ttgaaggatt
    15061 gtttatgtgg gacaagtctc tcgtttttac attttttagg aggcgttttg gaatggcatt
    15121 gtctactttt aagagagaac atattaaaaa gaatttaaga aatgatgaat atgatttagt
    15181 aattattggt ggcggtatta caggtgcagg tattgcacta gacgcgagtg aaagaggaat
    15241 gaaagttgca ttagttgaaa tgcaagactt tgcacaagga acaagctcaa gatctacaaa
    15301 attagtccat ggtggtttgc gttacttaaa acaattccaa attggagtag ttgccgaaac
    15361 tggtaaagaa cgtgcgattg tttatgaaaa tgggcctcat gttacgactc cagagtggat
    15421 gcttttacca atgcataaag gtggaacatt tggtaaattc tcaacatcaa ttggtttagg
    15481 aatgtatgat cgtttagcag gtgttaagaa gtctgaacgt aaaaaaatgt tatctaaaaa
    15541 agaaacttta gctaaagaac cattagttaa aaaagaaggt ctaaaaggcg gcggttacta
    15601 tgttgaatat cgtactgacg atgcgcgttt aactattgaa gttatgaagc gtgctgctga
    15661 aaaaggcgca gaaattatca actatactaa atctgaacac ttcacttatg ataaaaatca
    15721 acaagtaaat ggtgttaaag ttatagataa attaactaat gaaaattata caattaaggc
    15781 taaaaaagtg gttaatgcag caggtccatg ggttgatgat gttagaagtg gtgattatgc
    15841 acgcaataat aaaaaattac gtttaactaa aggtgtacat gttgttattg atcaatcaaa
    15901 attcccatta ggtcaagcag tatactttga tactgaaaaa gatggaagaa tgatttttgc
    15961 aattccacgt gaaggaaaag cgtatgtagg tactacagat acattctatg acaatatcaa
    16021 atcttcacca ttaactacac aagaagacag agactattta atcgatgcga ttaattacat
    16081 gttccctagt gttaatgtta cagatgaaga tattgaatca acatgggcag gaattagacc
    16141 attaatttac gaagaaggca aagacccttc tgaaatctct cgtaaggatg aaatttggga
    16201 aggtaaatca ggtttattaa ctattgcagg tggtaaatta acaggctatc gtcacatggc
    16261 tcaagacatt gttgatttag tatctaaacg cttgaaaaaa gactacggtt taacatttag
    16321 tccatgtaat acaaaaggtc tggcaatttc aggtggcgat gtaggtggta gcaagaactt
    16381 tgatgcgttt gtagagcaaa aagtagatgt agctaaagga ttcggcattg atgaagatgt
    16441 tgcaagacgt ttagcatcta aatatggttc aaatgttgat gaattgttca acattgcgca
    16501 aacatctcaa taccatgata gcaagttacc attagaaatt tatgtagaac ttgtttatag
    16561 tattcaacaa gaaatggtat acaaacctaa cgatttctta gttcgtcgtt ctggtaaaat
    16621 gtatttcaat attaaagatg tattagatta taaagatgct gtcatcgata ttatggcaga
    16681 tatgcttgat tactctccag ctcaaattga agcatatact gaagaagttg agcaagcaat
    16741 taaagaagcg caacatggaa ataatcaacc agcagttaaa gaataattaa tttgtacaat
    16801 cataaactgg tgtcctgttt taagggcatc agttttttta tacgagatac attagtcaat
    16861 tgaaactatg catcactaaa tgtatgataa taataaaagc gaatataaaa gcgttgttat
    16921 taaaggtgtg agatgatgac tgaaaaacaa tttaaattaa ctgtacaaga taatacgaat
    16981 attgaagtta aagtgaattt tacagatgta gattcaaaag gaattattca tatatttcat
    17041 ggtatggctg aacatatgga acgttacgat aaattagcac atgcactttc aaagcatggc
    17101 ttcgatgtga tacgtcataa tcatcgagga catggtatta atattgatga atcaacaaga
    17161 gggcattacg atgatatgaa acgagttatc ggtgatgcct ttgaagtagc gcaaacagtg
    17221 agaggcaatg ttgataaacc atacattata atcggacatt caatgggatc cgttatagct
    17281 agattgtttg tagaaacata tccgcaatat gttgatggtc taattttaag tggtactggt
    17341 atgtattcat tatggaaagg tttaccaacc gttaaagtgt tacaactgat tacaaaaatt
    17401 tatggtgctg agaaacgagt tgaatgggtt aaccagttag tatcaaatag ttttaataaa
    17461 aaaatacgtc cattacgtac acaaagtgat tggatttcta gtaatccaat tgaagtagat
    17521 aactttatta aagatccata tagtggattt aatgtgtcaa atcaattatt atatcaaaca
    17581 gcctattata tgctacatac atcacaatta aaaaatatga aaatgttaaa tcatgccatg
    17641 cctatattat tagtttcagg atatgacgat cctttaggtg attatggtaa agggatttta
    17701 aaattggcga atatatatag aaaagctggc attaaaaatg ttaaagtgaa tctttatcat
    17761 cataaacgtc atgaagtgtt atttgaaaaa gatcatgaca aaatttggga agacttgttt
    17821 aaatggttga atcaatttta taaaaaataa agaaagtgga attaaatatg aataaaaata
    17881 agccttttat tgtagtaatt gtggggccaa ctgcttcagg taaaacagag cttagcatcg
    17941 aactcgcgaa gcgtatcaat ggtgaaatca taagcggtga ctctatgcaa gtctacaaac
    18001 atatgaatat tggaactgca aaagtaacac ctgaagaaat ggatggtatt ccacatcatt
    18061 taattgatat cttgaatcct gatgatacat tttcagcata tgaattcaag cgattagcag
    18121 aagatttaat tactgatata acgaatagag gtaaagttcc aatcatagca ggtggaacag
    18181 gcttatatat tcaatcatta atatataatt atgaattaga agatgaaaca gttacacctg
    18241 cacaattatc catagttaaa caaaagttat ctgcattaga acatttagat aatcagcaac
    18301 tacacgatta tttagctcaa tttgatgcgg tttctgcaga aaatattcac cctaacaacc
    18361 gccaaagagt gttgcgcgct attgaatatt atttaaaaac aaaaaaactt ttgagtaatc
    18421 gcaagaaagt gcaacaattt actgaaaatt atgatacatt attattaggg attgaaatgt
    18481 cgcgtaaaac attatattca agaataaata aacgtgttga tattatgttg gatcacggat
    18541 tatttagaga agtgcaacaa cttgttgaac aaggctatga atcttgccaa agtatgcaag
    18601 ctattggata taaagaatta atacctgtga ttaacggaca aatgatttat gaagatgctg
    18661 tcaatgattt aaagcaacat tcacgccaat atgcaaaacg acaaatgaca tggttcaaga
    18721 ataaaatgag tgttcattgg ttagataaag aaaatatgtc acttcaaatg atgttagatg
    18781 agattacaac ccagattaag taaaaggagt ccgacagatg attgcaaacg aaaacatcca
    18841 agacaaagca ctagagaatt ttaaagcaaa ccaaactgaa gtaactgtat tctttctaaa
    18901 cggtttccaa atgaaaggtg ttattgaaga atacgacaag tatgtcgtaa gcttaaattc
    18961 tcaaggcaaa caacacttga tttacaaaca tgcgatcagc acttatacag tagaaactga
    19021 aggtcaagca tctactgaaa gtgaagaata agttgtaatc atatgggcac gatttaatga
    19081 ctcgtgtaca taacgtttga agtatgataa atagatatag aaataaagtc atatgtgaca
    19141 tcaattaaat gatgttcaaa tgacaagata caatagagga atgtttgtaa taaaaacgct
    19201 tcatataaag gtcgagtcaa tatatgatac gactttatat gaagcgtttt atttgctatg
    19261 agctagtata ttttataata atttttctat ttctctttcg atttgaacag gttttttttg
    19321 aggtgcaaat cgtttaacaa cgttaccttc gcgatccact aaaaacttag tgaaattcca
    19381 tttgattttc tcattaaaga atccgtgttg tgccgcagtc aaatatctaa ataaaggtaa
    19441 ttgatgttcc ccttttacgt ctattttttg atgcataggg aaggtaacac catagtttaa
    19501 tttacagttt tgagctgctt cttcgcctga accaggttct tggccaccaa attgattaca
    19561 agggaaacct agaattacaa acccttgatc tttgtatttc tcgtataatg attgcaaacc
    19621 ttcaaattgt gaagtaaagc cacattcgct agctgtatta acaattagca taacgtcacc
    19681 cttatatgca tctaatttgt aagtaacacc tttatttgtt tctactacaa aatcataaat
    19741 tgtctccatt gtatcatcct ttcgatttac ttaaaatgta ccacaaaatc gtgtaatagt
    19801 ctttactaaa actctatgat agaatacttt gagtaggatt ttattaagga gatgtataac
    19861 atggctcagc aacaaattca tgatactaaa aataaactag aaaaagctgt cttagtcggt
    19921 gtacatgctc aagatgataa gcaatttaat tttgagtcta caatggagga attatcatct
    19981 ttatcagaga cttgccaact tgaagtgttg ggtcaaatta ctcaaaacag agatcgtgta
    20041 gatcgcaaat attatgttgg taaaggtaaa attgaagaaa ttcaagcatt tattgagttc
    20101 aaagatattg atgtagtcat cacaaatgat gaattaacga ctgcacaatc caaatcacta
    20161 aatgaagctt taggtgtaaa aattattgat agaactcagt tgattcttga aatatttgca
    20221 ttaagagcaa gaagtaaaga aggtaaattg caagtagagc tagcacaact tgattattta
    20281 ttacctagat tgcaaggcca tggtaaaagc ctttctcgtt taggtggcgg tattggaact
    20341 agaggccctg gtgaaacgaa gttagagatg gatcgcagac atattcgaac tcgtatgaat
    20401 gaaattaaac atcaattgcg gacggtagaa gaacatcgcg aaagatatcg aaataaaaga
    20461 aatcaaaatc aggtgtttca agtagcttta gttggttata caaatgctgg taaatcatca
    20521 tggtttaatg ttttagcaaa tgaagagacg tatgaaaaag atcaattatt tgcaacgtta
    20581 gatcctaaaa cacgacaaat tcaaataaat gatggattta atttaattat ttcagatact
    20641 gttggtttta tacagaaact acctacgacg ttaattgcag cttttaaatc aactttagaa
    20701 gaggctaaag gtgcagattt attagtacat gtcgtagata gtagccatcc tgaataccgt
    20761 acgcagtatg acacagttaa tgatttaatc aaacaattag atatgagtca tatttctcaa
    20821 atagttattt ttaataaaaa ggacttatgt gatcatgcat caaatcgtcc agcaagtgat
    20881 ttgcctaatg tttttgtttc ttctaaaaat gatggtgata aattacttgt taagacgtta
    20941 tttattgatg aaatcaaaag gcaattaact tattatgatg agacaattgc gacgaataat
    21001 gcagatcgat tatattttct aaaacaacat acattagtga ctgaacttaa atatgatgaa
    21061 attgaaaatg tttatcgtat aaaaggattt aaaaaataat aaaaggacga aattcaaatg
    21121 aaagatataa gtaagatagt agctgacgtc gaatcaacgt tagcaccata ttttaaagaa
    21181 attgaagaaa cagcatatat taatcaagaa aaagtattaa atgcatttca tcatgtcaaa
    21241 gcaaccgaaa gtgatctaca aggatcaaca ggatacgggt atgatgactt tggacgtgat
    21301 catttagaag aaatatatgc gcaggcattt aaagcagaag atgcaattgt tcgtccgcaa
    21361 attatttcag gtacgcatgc gattactatt gcattacaaa gtttattaaa acatggtgat
    21421 gaattaattt atataacggg tagtccatat gacactttac ttgaagtcat tggcgtaaac
    21481 ggaaatggta ttgaaagttt aatggagcat ggcgtatcgt ataaagatat tgcacttaaa
    21541 gaaggtaaga tcgatattga aagtgtgtta gatggggttt ctgagcgcac caaagtaata
    21601 gcgattcaac gttcgaaagg ctatgatcaa agaccttcaa ttccgctaga tgaaattgaa
    21661 aaggtaatta ctaggttgaa aaacgtgcat cctaatattt taatatttgt ggataactgt
    21721 tatggggaat ttgttgaaag acgtgaacct atagaatgtg gtgccgattt aatagcagga
    21781 tcattaatta aaaaccctgg cggtggttta gctaagattg gtggatacat tgctggtaga
    21841 aaagatttaa ttgaacgatg tggttataga ttgacagcac ctggtattgg taaagaagcg
    21901 ggtgcatcat taaatgcatt gcttgaaatg tatcaaggtt tctttttagc accacacgtt
    21961 gtcagtcaga gtcttaaagg tgcattgttt actagtttat ttttagaaaa aatgaatatg
    22021 aacacaacgc cgaagtacta cgaaaaacga actgatttaa ttcaaacagt taaatttgaa
    22081 acgaaagaac aaatgatttc gttttgtcaa agtattcaac acgcatcccc aattaatgca
    22141 cattttagtc cagaacctag ttatatgcct ggttacgaag atgatgttat tatggcagct
    22201 ggtacgttta ttcaaggttc atcgattgaa ttatctgcag atggacctat tcgtcctcct
    22261 tatgaagcat atgttcaagg aggattaaca tatgaacacg ttaaaattgc tgtgacaaga
    22321 gctgttaatc agttgaaaga acaaggactt atataataga ttaaatttca ccctaaatga
    22381 taatgtgttt tgggtgaaat tttttgcgga gatttatcag attattttta aaaattgaaa
    22441 aaatatttta gctgtagatt gttttatgtc gttttagaag tcgaaatcgc agttatatca
    22501 atgttttaag ctttatgtta gaaaacctga catatttttg aaatcctaaa aaaattatga
    22561 taagttatta acaagttcaa aagtagagga gaggaacaat gatatcgaat gatgcaatca
    22621 gacgaaatat ggctgtcttc tctatgagtg tagtaagtaa gttaacggat ttaacgccaa
    22681 ggcaaatacg ttactatgaa acacatgaac tcatcaaacc tgaaagaaca gaaggtcaaa
    22741 aacgtctgtt ctcactcaat gatttggaaa gattactaga aattaaatca ttattagaaa
    22801 aaggatttaa tatcaaaggg attaaacaaa tcatttatga ctcacaagag catttaacaa
    22861 cagatgaaca agagataaga aaaaagatga ttgtagatgc cacgcaaaag cctattggag
    22921 aaactttgcc aataaatcgt ggtgatttat cccgatttat taaataaaat ttggaggatt
    22981 ttaaaatgcc aaaacgtact ttcactaaag acgacattcg taaatttgca gaagaggaaa
    23041 atgtaagata tttaagatta caattcactg atattttagg aacaattaaa aatgttgaag
    23101 tgcctgtaag ccaattagaa aaagtacttg ataacgaaat gatgtttgac ggttcttcta
    23161 tcgaaggttt cgtacgtatc gaagaatcag atatgtactt acatccagat ttagatactt
    23221 gggtaatctt cccatggact gctggacaag gtaaagttgc acgtttaatt tgtgatgtat
    23281 ataaaacaga tggaacacca tttgaagggg atcctcgtgc aaacttaaaa cgtgtattaa
    23341 aagaaatgga agatttaggc ttcacagact ttaacctagg gcctgaacca gaattcttct
    23401 tgtttaagtt ggatgaaaaa ggggaaccaa ctttagaact taatgatgat ggtggatatt
    23461 tcgatttagc acctacagat ttaggtgaaa actgtcgtcg tgatattgtt ttagaattag
    23521 aggatatggg cttcgatatt gaagctagtc accatgaagt tgcccctggt caacatgaaa
    23581 ttgactttaa atatgcagat gctgttacag catgtgataa tatccaaaca tttaaattgg
    23641 ttgttaaaac aatcgcacgt aaacataatt tacacgcaac atttatgcct aaaccattat
    23701 tcggtgtgaa tggtagcggt atgcacttta acgtttcatt attcaaaggt aaagaaaatg
    23761 cattctttga tccaaatact gaaatgggct taacggaaac tgcatatcaa tttacagcag
    23821 gtgtgcttaa aaatgcacgc ggatttactg ctgtatgtaa cccgttagta aactcatata
    23881 aacgtttagt acctggttat gaagcaccat gttatattgc atggagtggt aaaaaccgtt
    23941 caccattaat ccgtgtacca tcttcaagag gattatctac tcgtatcgaa gtacgttcag
    24001 tagatccagc tgcaaaccca tacatggcgt tagctgcaat cttagaagct ggactagatg
    24061 gtattaaaaa taaattaaaa gttccagaac cagttaacca aaatatttac gaaatgaacc
    24121 gtgaagaacg tgaagcagta ggcattcaag acttaccttc aacactttat actgcattaa
    24181 aagcaatgcg tgaaaatgaa gttattaaaa aagctttagg aaatcatatc tataatcaat
    24241 ttattaattc aaaatcaatt gaatgggatt actacagaac tcaagtctct gaatgggaaa
    24301 gagatcagta catgaagcaa tattaatacg ctaaacaatg atgggaaatc agtaatatca
    24361 agggctagag cattttgctt tagctctttt ttgatttgga aagatgggat ttcacttgac
    24421 cacttttgtt aaaaaatgga tttggtcaag ttttggtcaa gtaaatgttt tatgtattta
    24481 tttttttaaa gctggggaag accattttat attttagttc acatgaattt taatatattt
    24541 ggaggtgatg agatgaggcg ataaaagaac gtcgcgaaaa cgttgagtat tacttgaaaa
    24601 tagccatatt catattactt ctcatcgaat tcatttgcag aacgttctaa ggtaagaccc
    24661 cgaaaagagg gaccttacta cgcattatta agggggtaat actatatgaa aagagaaaca
    24721 aaacaaaaaa tatctttttg tttatcgatc ggtattttca ttttagttat actactattg
    24781 atattctaaa aaattaaaaa tttcgcctca tctaatttat gaattgctat gatgaaatat
    24841 tcaatacaat caaagaattg atagaaaaca aagagatatc gagttatcaa attaataaag
    24901 atactgggat aagttacggt aatattaatg ctatgcgccg tagagaaaga agaatagaaa
    24961 atttaagctt aaagaatgca aagatcttat atgaatatgc gaaaaaggta ttgtaatata
    25021 agcattgagt tcaataggca tacaataact gcccaaacta gtgactgagt gggtgctttt
    25081 atagtattaa aagttttcta aaagtgaaag aaatcatgtt tagtgattta cttatgtttt
    25141 tcaaaatagt ataataattt tatataatat atagagcttc agtatacctt ttgagtatat
    25201 atggagttct ttctttttat acaagatgta tatgtataaa tggaaatatt taatacatta
    25261 cataaaaacc tttaggtgca cttagaggga tgttaatcat aaaaaaagag attttgaaaa
    25321 atataggcta ttatacttta gaattacaaa ttataaatct tgccagtgga tatgggagtt
    25381 caacatgtca cttaccccaa tttgtggaat gttaaataac tataaagagg aaaggagaaa
    25441 aaatgataag aatggttaaa gaaaaagaac gaatagcaaa attaaatttc gaatagctgt
    25501 gacgagctta ataattcaag tacttaattt tatcaaatcg ttcttttaaa aaggtaaagg
    25561 gcaaaagacc tttaccacat taaataggga ggtggtattt atgacaagag aattaaggaa
    25621 aaaattaact ctctatc
//
LOCUS       DALAGF010000030        25545 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.030, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000030 DALAGF010000000
VERSION     DALAGF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25545
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.030"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            100..327
                     /locus_tag="O2692_001149"
     CDS             100..327
                     /locus_tag="O2692_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298785.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
     gene            complement(632..1579)
                     /locus_tag="O2692_001150"
     CDS             complement(632..1579)
                     /locus_tag="O2692_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCZ4298786.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLKNVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLLLAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKEKQDKFLRNASQ"
     gene            1828..2016
                     /locus_tag="O2692_001151"
     CDS             1828..2016
                     /locus_tag="O2692_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCZ4298787.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANSIFSNEN"
     gene            complement(2491..2564)
                     /locus_tag="O2692_001152"
     tRNA            complement(2491..2564)
                     /locus_tag="O2692_001152"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2528..2530),aa:Arg,seq:tct)
     gene            2928..3062
                     /locus_tag="O2692_001153"
     CDS             2928..3062
                     /locus_tag="O2692_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCZ4298788.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            3119..3253
                     /locus_tag="O2692_001154"
     CDS             3119..3253
                     /locus_tag="O2692_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCZ4298789.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            3377..4072
                     /locus_tag="O2692_001155"
     CDS             3377..4072
                     /locus_tag="O2692_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCZ4298790.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     EINSWKQLNDIVR"
     gene            complement(4181..4621)
                     /locus_tag="O2692_001156"
     CDS             complement(4181..4621)
                     /locus_tag="O2692_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ4298791.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            4816..6429
                     /gene="bshC"
                     /locus_tag="O2692_001157"
     CDS             4816..6429
                     /gene="bshC"
                     /locus_tag="O2692_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HCZ4298792.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            6573..7004
                     /gene="mraZ"
                     /locus_tag="O2692_001158"
     CDS             6573..7004
                     /gene="mraZ"
                     /locus_tag="O2692_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCZ4298793.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            7020..7955
                     /gene="rsmH"
                     /locus_tag="O2692_001159"
     CDS             7020..7955
                     /gene="rsmH"
                     /locus_tag="O2692_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCZ4298794.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            7969..8370
                     /gene="ftsL"
                     /locus_tag="O2692_001160"
     CDS             7969..8370
                     /gene="ftsL"
                     /locus_tag="O2692_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCZ4298795.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            8351..10585
                     /locus_tag="O2692_001161"
     CDS             8351..10585
                     /locus_tag="O2692_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCZ4298796.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            10877..11842
                     /locus_tag="O2692_001162"
     CDS             10877..11842
                     /locus_tag="O2692_001162"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCZ4298797.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            11844..13193
                     /locus_tag="O2692_001163"
     CDS             11844..13193
                     /locus_tag="O2692_001163"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCZ4298798.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVNGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            13209..14528
                     /locus_tag="O2692_001164"
     CDS             13209..14528
                     /locus_tag="O2692_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCZ4298799.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEESNDKNNDSASSHANDNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNVISVDNEQPQSAPKEQNSDSIDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEENPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            14634..16046
                     /gene="ftsA"
                     /locus_tag="O2692_001165"
     CDS             14634..16046
                     /gene="ftsA"
                     /locus_tag="O2692_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCZ4298800.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            16079..17251
                     /gene="ftsZ"
                     /locus_tag="O2692_001166"
     CDS             16079..17251
                     /gene="ftsZ"
                     /locus_tag="O2692_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCZ4298801.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
     gene            17511..18302
                     /gene="pgeF"
                     /locus_tag="O2692_001167"
     CDS             17511..18302
                     /gene="pgeF"
                     /locus_tag="O2692_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HCZ4298802.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LIYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            18326..18994
                     /locus_tag="O2692_001168"
     CDS             18326..18994
                     /locus_tag="O2692_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCZ4298803.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            18991..19554
                     /locus_tag="O2692_001169"
     CDS             18991..19554
                     /locus_tag="O2692_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCZ4298804.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            19566..19856
                     /locus_tag="O2692_001170"
     CDS             19566..19856
                     /locus_tag="O2692_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCZ4298805.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            19966..20745
                     /locus_tag="O2692_001171"
     CDS             19966..20745
                     /locus_tag="O2692_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCZ4298806.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            20769..21386
                     /locus_tag="O2692_001172"
     CDS             20769..21386
                     /locus_tag="O2692_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCZ4298807.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK"
     gene            21607..24360
                     /gene="ileS"
                     /locus_tag="O2692_001173"
     CDS             21607..24360
                     /gene="ileS"
                     /locus_tag="O2692_001173"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCZ4298808.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            24650..25447
                     /locus_tag="O2692_001174"
     CDS             24650..25447
                     /locus_tag="O2692_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4298809.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNNLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
BASE COUNT         9432 a         3490 c         4814 g         7809 t
ORIGIN      
        1 attatattta tcgtggtaaa tatgtatttg ctgtaaacag tttttgtaac acatgataca
       61 tatggttgtg aaaatagcat atataaagga tggctataaa tgacacattt gacaaaggtt
      121 ttagatacac taactggaat atgcgtagta ttattattta gtaaatattt tgtggcgtat
      181 gcaaatatgg tgtttgattg gaatttaaga tggtatttgc tagaaaacat accacatttg
      241 ccaattatat tatttattct gatgtttatt ttcggagtac cttctgaaat gataaaagat
      301 aggcaaagga aaaataacgg tgtttaattt atcgatattt agaggtgata aaatttgtca
      361 actattacaa agactaaaat aaaaatcttt attaattatt ttattggtat attagccttg
      421 gcgtttgctg cttatatatt atttaaataa agtttagagt gaaacgtgtt tatgaactag
      481 acgttctagt atagttacta tagctttagt cagaaggtat cattgataag atcatattaa
      541 atcaaagagg cattgatata cactaaaaag aggcaagatt acctgcctct ttttagttat
      601 taaatatacg tgttaattct tggtaatagt gttactgaga cgcattacga agaaatttat
      661 cttgtttttc cttttttaaa aagaagtgaa gatatcctat aaagactcta agtactatta
      721 cagtggctaa taacacttcg ataaacaaaa gacctttcca aatgtctgga aacataagta
      781 caggcaaact gttctttaaa gcagttgctg agattactaa agggaatgtg aaagctgaaa
      841 atacgggcga aaaaggttct tttagtaatt taggcaattg tataatgata taaaaataaa
      901 atatttgagc taatagtaaa aatataatta cgataaaagc attcgcctta ggaaaagcta
      961 taacatatgc tgcagcgact aaagaaaatg gtgcacaaat tgtcgatgtg ttcggtttga
     1021 ttgacgtctg caatggaaat gcttttaatc gcttgaaaac tataggtaag acaatacaag
     1081 tagctacaaa gccatatatt actgttaatt gacctatgaa aaaatatccg ctaacgggtg
     1141 ccgtcaatcc tgcgatagca ataccaataa aaagtacagt ccacgaagga taaacatttt
     1201 taagtgaaaa atcttttaaa tattttattg aaaaaataat catatgcgtc ataattccca
     1261 caaggcataa aatccaaata ggcgttatta agctattgat aaaagttatg ttactaaaaa
     1321 atgtatttaa ataagtagta cctaaaaagc cagacatgaa aaatgttgtg aacactgatg
     1381 aaactagagg actgtttaat tgttctttca cattattaaa atatttgatc atagtacata
     1441 aaaggtgaat ccaaatcaag aaagcaaaga ttccgcaaac agcgtttaaa gtaagagata
     1501 agtcttttaa tagattaccc aggcctaata atcctaagac tagtcctgac gttactagag
     1561 gtgctttttg aagtctcatg atttacaacc tttcttatgt gattttcttc actaattata
     1621 tcatgatagc tatggccaat taataagaag agtgtgtact attacgttat tagagtatgt
     1681 atattggatt gatagacaca taattgacat ttaaatctca aaattaatga tatttgaggt
     1741 atgctttgca acctaaatat tggaatatgt ggaaaagtaa ttatttaatt taaatgatga
     1801 ttgaagattt acaagggggt gtacaaaatg agaaatcaaa ttcaaaaact attagacagt
     1861 gatttgagca gtttacatat atcgaaacaa acaggagttc cacaaagcac aatacacaga
     1921 atgagaaaaa atgaaagatc attagacaat atgtcattga aaaacgctga actactttat
     1981 aaatttgcca atagtatatt tagcaatgaa aattaagaag aataattagt gagttaaata
     2041 attaaaaaac cacgtctcga tggacgtggt tatttttttt agatgtatat aattataatt
     2101 ataggatttc gggcccctga aaagtcccta aaattttgaa tagtatcaaa atctatagga
     2161 ataatataat aataaaaatc tctacgcatg caaatggtga gtgttaaaaa tcttggtgta
     2221 gtgttggtgt agttcaggtg cagtatagat gaaatttgag cacaaaatta cttaattaaa
     2281 aatgtataaa caagtgtcag acgcgtgtca attgtgtgtc aaaaaattct ataacgaata
     2341 agcatgcttg ttttaattac tgtattttcg gggcttatgc gggggacgtt ttggtgacgc
     2401 atcatactat attactgaaa ttcaaaacaa aaagagcccc gtaatcacgg aactcttttg
     2461 tttggtaatg cgtataaaaa tacctataaa cgtcctggga gggattcgaa cccccgaccg
     2521 atggcttaga aggccattgc tctatccagc tgagctacca ggacacgttt aacaacacaa
     2581 gaattattat atctaaatga acttaaatta gcaatacctt gtaaataaaa aatgtttata
     2641 tttttcacta ttatagagct atttatctaa aaaggttcaa taagacttaa atacgaattc
     2701 aggcaactta attgtgttaa atacagtttt gaatgcctaa ctgtatttct tttctcttta
     2761 aaatacagtt aagtacatta taagatgttg tgcggataaa caaactaatt gtatcaaatt
     2821 tattttaaaa taacaacaac aaaacgataa gcgaataaca tttcggtgat ttaaaagcta
     2881 cgcacgtttt tgttatcttc aaatttaaat tttaaggagt gttttcaatg gaaggtttat
     2941 ttaacgcaat taaagatacc gtaactgcag caattaataa tgatggcgca aaattaggca
     3001 caagcattgt gagcatcgtt gaaaatggcg taggtttatt aggtaaatta ttcggattct
     3061 aatttcaata tgttatgtaa gtaatcagta ttatttcaaa ggtgagggag agatttaaat
     3121 gactggacta gcagaagcaa tcgcaaatac tgtgcaagct gcacaacaac atgatagtgt
     3181 gaaattaggc acaagtatcg tagacatcgt tgctaacggt gtgggtttac taggtaaatt
     3241 atttggattc taatataata actaatattc tttaaaataa actgggtgag catactttaa
     3301 tgttatgcac tcagtttatt ttatttgcag aaatttgagc ctctgttaag atttagatac
     3361 atagacaata taggagatgg ggaaattggg atataaaaat attttgatag actttgatga
     3421 tacaattgtt gatttttatg atgcagaaga atgggcgttt cactatatgg cgaatgtttt
     3481 taatcataaa gcaacaaagg atgatttttt aacatttaaa aaaatcaatc accaacattg
     3541 ggaagctttt caacaaaata aattaacgaa gtctgaagta ttatcagaac gatttgtgaa
     3601 ttacttcaaa catcatcaaa tggaagttga tgggcatcgt gcagatgtgt tatttagaaa
     3661 tggattagca gaagctaaag ttaaatactt tgatcaaaca ttagaaacaa ttgtcgaatt
     3721 atcgaaaaga catgatttat atattgttac taatggtgta accgaaacgc aaaagagaag
     3781 gttaaatcag acgccgttgc ataaatatat taaaaagata tttatatctg aagaaacagg
     3841 atatcaaaaa cctaatccgg aattttttaa ttatgttttt aatgatattg gtgaggatga
     3901 aagacagcac tcgattatag ttggagattc tttaacatct gacattctag gtggaatcaa
     3961 tgcgggtata gctacttgct ggtttaattt tagaggattt gatcataatc caggaattat
     4021 acctgattat gaaattaatt catggaaaca actaaatgat attgtacgtt aagacaaata
     4081 atttgaaaat gatttactta tattagctta agaaagtaat tgtaaaaaag acactacgaa
     4141 aagatgcatt ttcatgctcg tagtgtcgaa tattattatc ttatggtttc cacaataaga
     4201 catcatgtcc ctctatattt ttagcttcca catctgtaaa tccatgatgt aaaaagaaat
     4261 ccttagaatc atttctgcca atggctttaa ttggcatatt gaaactcttt gcaaaatcaa
     4321 tcaattctga agcgtaccct ctgttttgat attttggtaa tacttctaac ttccataata
     4381 atatataatc ttcaaaatct gggaagtaga tttcttcgac atcacctttt tttagtaatg
     4441 ccattctagc tcctaattga tctccgacaa atatgccata aaatggtgaa tctgaacttg
     4501 catcaatcat ttgaccgttt aactcattga ccatgtataa gtctttgttg ccaaacgctc
     4561 taaagttttc gaataattcg tcagttttgt aattaatttc aagacgtttg atttcactca
     4621 tatttatcta ccccttattt gtttactact attattatac attaaaccac ttgtgttttt
     4681 cattgatttg aaaatgtaaa acaatgaata ttataaattt tccatgatta aattgatttt
     4741 caagccgtaa aattgtcaat caccggtata gactttatac tatagtttgt aatcgcgtac
     4801 ttaaggagaa aaaacatgga ctgtaaagta gttagtttaa atgaaaaaga tcagtttata
     4861 ccaaaaataa agagcagtga ccctgtaata acaggattat ttcaatatga tgcagctcaa
     4921 caaactagtt ttgaaaaaag gatgtctaaa gaaaataatg gaagagaagc ggcattagcg
     4981 aatgttattc gtgaatatat gagtgattta aagctttcaa gtgaacaaga attaaacata
     5041 caacatttag ctaatggttc aaaagttgtg attggtggac aacaagcagg gcttttcggg
     5101 ggaccattgt atacattcca taaaatattt tcaatcatta ctttatctaa ggaattaacg
     5161 gatacacata agcaacaagt agtaccagtt ttttggattg caggagaaga tcatgatttc
     5221 gatgaagtga atcatacatt tgtttataac gaaaatcatg ggtcgctgca taaggttaaa
     5281 tatcatacaa tggagatgcc agagacgact gtctctagat attatcctga taaggctgag
     5341 ttgaaacaaa ctttaaaaac gatgttcatt catatgaaag aaactgttca tacacaaggt
     5401 ctactggaga tttgtgacag aattattgac caatatgact cgtggactga tatgtttaaa
     5461 gcactactgc atgaaacatt taaagcatat ggcgttctat ttatagatgc gcagtttgag
     5521 ccgttaagaa aaatggaagc gcctatgttt aaaaagattt tgaaaaaaca tcagttgctt
     5581 gatgatgctt ttagagcaac acaacaacgt actcaaaatc aaggcttgaa tgcgatgata
     5641 caaacagata caaatgttca tttattctta catgatgaaa atatgcgtca attagtttcg
     5701 tatgatggta agcattttaa attaaataaa acagataaga catatataaa ggaagaaatt
     5761 ataaatattg cggaaaatca acctgaatta ttttctaata atgtagtgac aagaccatta
     5821 atggaagaat ggttatttaa cacggtggca tttgttggag gaccgagtga aattaagtac
     5881 tgggctgaac taaaagatgt atttgaacta tttgatgttg aaatgcctat cgtgatgcca
     5941 aggcttagaa ttacttattt aaatgaccgt atagaaaaat tactttcgaa atacaatatt
     6001 ccattagaaa aagtgttagt cgatggtgtt gaaggagaaa gaagtaagtt tattagagaa
     6061 caagcatcac atcaatttat tgaaaaggta gaaggtatga ttgaacaaca gcgtcgtcta
     6121 aacaaagact tattagatga agtggcgggg aatcaaaata atattaacct tgtgaataaa
     6181 aataatgaaa ttcatataca acagtatgat tatttgttaa aacgttatct tttaaacatt
     6241 gaaagagaaa acgacatcag tatgaagcaa tttagagaaa ttcaagaaac actccatcca
     6301 atgggaggat tacaagaaag aatatggaat ccacttcaaa ttttgaatga ttttgggaca
     6361 gatgtgttca agccctccac ctatccacca ctttcttaca cttttgatca tattattata
     6421 aaaccttaat ataccaaggg tttagcccga tttatcttaa tgataaatcg ggcatttttt
     6481 tgttttttaa aataaatttc acaaattttg tataaatagt ggtggatagt ggggagatgt
     6541 ggtaaattat atataaggtg aggtgataaa aaatgttcat gggagaatac gatcatcaat
     6601 tagatacaaa aggacgtatg attataccgt ccaagtttcg ttatgactta aatgagcgtt
     6661 ttattatcac aagaggcctt gataaatgtt tattcggtta cactctagac gaatggcaac
     6721 agattgaaga gaaaatgaaa accttaccta tgacaaaaaa agacgcacgt aagtttatgc
     6781 gtatgttctt ctctggtgct gttgaagtag aacttgataa gcaagggcgt attaacatcc
     6841 ctcaaaactt gaggaaatac gctaatttaa ctaaagaatg tacagtaatc ggtgtttcaa
     6901 atcgtattga gatttgggat agagaaactt ggaatgattt ctatgaagaa tctgaagaaa
     6961 gtttcgaaga tattgctgaa gatttaatag attttgattt ttaaaatgga ggaattgaag
     7021 tgtttcatca tatcagcgtt atgttaaacg aaaccattga ttatttaaat gtaaaagaaa
     7081 atggtgtgta cattgactgt acgctaggtg gagcgggaca tgccctttat ttactaaatc
     7141 aattaaatga cgacggaaga ttaatagcaa tcgatcaaga ccaaactgca attgataatg
     7201 ctaaagaggt attaaaggat catttgcata aggtgacttt tgttcatagc aacttccgtg
     7261 aattaactca aatattaaaa gacttaaaca ttgaaaaagt agatggaatt tattacgact
     7321 tgggtgtttc aagcccacaa ctcgacattc cagaacgagg attcagttat caccatgacg
     7381 caacattaga catgcgtatg gaccaaacac aagaactaac agcatatgaa attgttaaca
     7441 attggtcata tgaagcgtta gtgaagattt tttatcgcta tggcgaggag aaattttcaa
     7501 aacagatagc tcgaagaatc gaagcacatc gcgaacaaca accaataaca acaacattag
     7561 aattagttga cattataaaa gaaggtattc ctgcaaaagc aagaagaaaa ggcggacatc
     7621 ctgcaaaacg agtatttcaa gcactacgaa ttgcagtaaa cgatgaattg tcagcttttg
     7681 aagattcaat agaacaagcg attgaattag tgaaagtaga tggcaggatt tcggtaatca
     7741 ctttccattc tttagaagat cgtttatgta aacaggtgtt ccaagaatat gaaaaaggtc
     7801 cagaggtacc aagaggatta ccagttatac cagaagcata tacacctaag ttaaagcgtg
     7861 ttaatcgtaa accgattacc gctacagaag aagatttaga tgacaataac agagcacgaa
     7921 gcgcgaaatt acgtgtagct gaaatactta aataaggagc aatttataat ggctgtagaa
     7981 aaagtgtacc aaccatatga cgaacaagtt tataatagta taccgaagca acaaccacaa
     8041 actaagcccg aaaagaagac tgtttcgaga aaagtggttg tacaattaac taaatttgaa
     8101 aaagttttat acataacttt gattactgta attgctatgt taagtattta tatgctatct
     8161 ttaaaaatgg atgcgtatga tacgcgagga aagattgcag atttagatta taaaatagat
     8221 aaacaatcaa gtgaaaacag tgctttacaa tctgaaatca aaaagaattc ttcttatgaa
     8281 cgcatatacg aaaaggctaa gaaacagggg atgagccttg agaacgataa tgtaaaggta
     8341 gtgcgtagta atggcgaagc aaaaaattaa aattaaaaaa aataaaatag gggcagtcct
     8401 acttgttggt ttattcggac tgctcttttt tatattggtt ttaagaattt catatatcat
     8461 gattactgga cattctaatg gtcaagattt agtcatgaag gcaaatgaaa agtatttagt
     8521 taagaatgca caacaaccag aacgaggaaa gatatatgat cgtaatggta aagtgctagc
     8581 agaagatgta gaaagatata aacttgttgc agtaatagat aaaaaggcga gtgccaattc
     8641 taaaaaacct aggcatgtag ttgataaaaa agagactgca aagaaattat ctacagtcat
     8701 taatatgaag ccagaggaaa ttgaaaagag acttagtcaa aagaaagctt tccaaattga
     8761 atttggacgc aaaggaacaa atttaacgta tcaggacaaa ttgaaaatag agaaaatgaa
     8821 tttgcctggt atttctttat tgcctgaaac agaacgcttt tatccaaatg gcaattttgc
     8881 atcacactta attggtagag ctcagaaaaa tccggatact ggtgaactta aaggtgcact
     8941 tggagttgaa aagatttttg atagttattt aagtggatct aaaggatcat tgagatatat
     9001 tcatgatatt tggggatata tcgcaccaaa tactaaaaaa gagaagcagc ctaaacgtgg
     9061 tgatgatgtc catttaacaa tcgattcaaa tattcaagta tttgttgaag aagctttaga
     9121 tggcatggtt gaaagatacc agccgaaaga tttatttgcg gttgtcatgg atgccaaaac
     9181 tggagaaatt ttagcataca gtcagcgacc aacatttaat cctgaaactg gtaaagactt
     9241 tggtaaaaag tgggcaaatg acctttatca aaacacatac gagcctggat caacatttaa
     9301 atcatatggg ttagcagctg ctattcaaga aggtgctttt gatcctgata agaaatataa
     9361 atctggacat agagatatta tgggttcacg tatttcagac tggaatagag tcggttgggg
     9421 tgaaatccca atgtcactcg gatttactta ttcatctaat acattgatga tgcatttaca
     9481 agatttagtt ggtgcagaca aaatgaaatc ttggtatgaa cgatttggat ttggaaaatc
     9541 aactaaaggt atgtttgatg gagaagcacc tggtcaaatt ggatggagta atgagttgca
     9601 acaaaaaacg tcatcatttg gtcaatcgac aacagtaaca cctgttcaaa tgttacaagc
     9661 gcaatcagcg ttctttaatg atggtaatat gttaaaacca tggtttgtga atagcgttga
     9721 aaatcctgtt agtaaaagac aattttataa agggcaaaaa caaatcgcag gcaaaccaat
     9781 aacaaaagat actgctgaaa aagttgaaaa gcaattggat ttagttgtga atagtaagaa
     9841 gagtcacgct gcaaactatc gtattgatgg ttatgaggtc gaaggtaaga ctggtacagc
     9901 acaagtcgct gcacctaatg gtggtggata cgttaaaggt ccaaacccat attttgtaag
     9961 ttttatgggt gacgcgccga agaaaaatcc taaagttatt gtatacgctg gtatgagctt
    10021 ggcacaaaaa aatgaccaag aagcttatga attaggtgtt agtaaagcgt ttaaaccaat
    10081 aatggaaaat actttgaaat atttaaatgt aggtaaatca aaagatgaca catctaatgc
    10141 agagtatagt aaagtgccag atgttgaagg tcaagacaaa caaaaagcta ttgataatgt
    10201 gagtgcaaaa tcattagaac cagttactat tggttctggc acacaaataa aagcacaatc
    10261 tataaaagca gggaataaag tcttacctca tagtaaagta ctgttattaa cagatggaga
    10321 cttaactatg cctgacatgt caggatggac gaaagaagat gtcattgctt ttgaaaacct
    10381 aacaaatatt aaagtaaatt taaaaggtag cggttttgtg tcccaccaat caattagtaa
    10441 gggacaaaaa cttactgaaa aagataaaat agacgtagaa ttttcatcag agaatgtaga
    10501 cagcaattcg acgaataatt ctgattcaaa ttcagatgat aagaagaaat ctgacagtaa
    10561 aactgacaag gataagtcgg actaacaaga atggctacat taaattagga tgtattgtca
    10621 ctatttgttt ttactaaagt aatgctgtga tagaaaacaa catcttcatt taatctttat
    10681 aaataagtgt tgatagagaa ttttaaggta gacgaggtca gattaacttg tttgttatag
    10741 gcaaactttt tatactggcc tcttatgccg taaaaaagaa aaattataaa ttttgattac
    10801 aactttgttt atttaattga aaaattgata atatattagt ggttaaaaaa gtctggatta
    10861 agaagaggag atagttatga tttttgtata tgcgttatta gcgctagtga ttacatttgt
    10921 tttggtacct gttttaatac ctacattaaa aaggatgaaa tttggtcaaa gtattcgaga
    10981 agaaggtcca caaagccata tgaagaagac tggtacacca acgatgggtg gactaacatt
    11041 tctattaagt attgtgataa cgtctttggt ggctattata tttgtagatc aagctaatcc
    11101 aatcatactg ttattatttg tgacgattgg ttttgggtta attggtttta tagatgatta
    11161 tattattgtt gttaaaaaga ataaccaagg tttaacaagt aaacagaagt ttttggcgca
    11221 aattggtatt gcgattatat tctttgtttt aagtaatgtg tttcatttgg tgaatttttc
    11281 tacgagcata catattccat ttacgaatgt agcaatccca ctatcatttg catatgttat
    11341 tttcattgtt ttttggcaag taggtttttc taatgcagta aatttaacag atggtttaga
    11401 tggattagca actggactgt caattatcgg atttacaatg tatgccatca tgagctttgt
    11461 gttaggagaa acggcaattg gtattttctg tatcattatg ttgtttgcac ttttaggatt
    11521 tttaccatat aacattaacc ctgctaaagt gtttatggga gatacaggta gcttagcttt
    11581 aggtggtata tttgctacca tttcaatcat gcttaatcag gaattatcat taatttttat
    11641 aggtttagta ttcgtaattg aaacattatc tgttatgtta caagtcgcta gctttaaatt
    11701 gactggaaag cgtatattta aaatgagtcc gattcatcat cattttgaat tgataggatg
    11761 gagcgaatgg aaagtagtta cagtattttg ggctgttggt ctgatttcag gtttaatcgg
    11821 tttatggatt ggagtgcatt aagatgctta attatacagg gttagaaaat aaaaatgtat
    11881 tagttgtcgg tttggcaaaa agtggttatg aagcagctaa attattaagt aaattaggtg
    11941 cgaatgtaac tgtcaatgat ggaaaagact tatcacaaga tgctcatgca aaagatttag
    12001 aatctatggg catttctgtt gtaagtggaa gtcatccatt aacgttgctt gataataatc
    12061 caataattgt taaaaatcct ggaatacctt atacagtatc tattattgat gaagcagtga
    12121 aacgaggttt gaaaatttta acagaagttg agttaagtta tctaatctct gaagcaccaa
    12181 tcatagctgt aacgggtaca aatggtaaaa cgacagttac ttctctaatt ggagatatgt
    12241 ttaaaaaaag tcgcttaact ggaagattat ccggcaatat tggttatgtt gcatctaaag
    12301 tagcacaaga agtaaagcct acagattatt tagttacaga gttgtcgtca ttccagttac
    12361 ttggaatcga aaagtataaa ccacacattg ctataattac taacatttat tcggcgcatc
    12421 tagattacca tgaaaattta gaaaactatc aaaatgctaa aaagcaaata tataaaaatc
    12481 aaacggaaga ggattatttg atttgtaatt atcatcaaag acaagtgata gagtcggaag
    12541 aattaaaagc taagacattg tatttctcaa ctcaacaaga agttaatggt atttatatta
    12601 aagatggttt tatcgtttat aaaggtgttc gtattattaa cactgaagat ctagtattgc
    12661 ctggtgaaca taatttagaa aatatattag cagctgtgct tgcttgtatt ttagctggtg
    12721 tacctattaa agcaattatt gatagtttaa ctacattttc aggaatagag catagattgc
    12781 aatatgttgg tactaataga actaataaat attataatga ttccaaagca acaaacacgc
    12841 tagcaacaca gtttgcctta aattcattta atcaaccaat catttggtta tgtggtggtt
    12901 tggatcgagg gaatgaattt gacgaactca ttccttatat ggaaaatgtt cgcgcgatgg
    12961 ttgtattcgg acaaacgaaa gctaagtttg ctaaactagg taatagtcaa gggaaatcgg
    13021 tcattgaagc gaacaatgtc gaagacgctg ttgataaagt acaagatatt atagaaccaa
    13081 atgatgttgt attattgtca cctgcttgtg cgagttggga tcaatatagt acttttgaag
    13141 agcgtggaga gaaatttatt gaaagattcc gtgcccattt accatcttat taaagggtgt
    13201 gagtattgat ggatgataaa acgaagaacg atcaacaaga atcaaatgaa gataaagatg
    13261 aattagaatt atttacgagg aatacatcta agaaaagacg gcaaagaaaa agatcaaagg
    13321 ctacacattt ttctaatcaa aataaagatg atacatctca acaagctgat tttgatgaag
    13381 aaatttactt gataaataaa gacttcaaaa aagaagaaag caatgataaa aataatgatt
    13441 ctgcttctag tcatgcgaat gataataata tcgatgattc tacagactct aatattgaaa
    13501 atgaggatta tagatataat caagaaattg acgaccaaaa tgaatcgaat gtaatttcag
    13561 tcgacaacga acaacctcaa tcagctccta aagaacaaaa tagcgactcg attgatgagg
    13621 aaacagtaac gaaaaaagaa cgaaaaagta aagtaacaca attaaagcca ttaacacttg
    13681 aagaaaagcg gaagttaaga cgtaagcgac aaaagcgaat ccaatacagt gttattacaa
    13741 tattggtatt gttgattgct gttatattaa tttacatgtt ttcaccactt agtaaaattg
    13801 cgcatgtaaa tataaatgga aataatcacg ttagtacttc aaagataaac aaagttttag
    13861 gtgttaaaaa tgattcaagg atgtatacgt ttagtaaaaa aaatgctatt aatgatctcg
    13921 aagagaatcc attaatcaaa agtgttgaga tacacaagca attaccaaac acattaaacg
    13981 tagatatcac agaaaatgaa attattgctt tagtgaaata taaaggtaaa tatttacctt
    14041 tattagaaaa tggtaaattg cttaaaggtt caaatgatgt caaaattaat gatgcacctg
    14101 tcatggatgg tttcaaaggt acaaaagaag atgatatgat taaggcgtta tctgaaatga
    14161 cacctgaagt tagacgatat attgccgaag tgacatacgc cccaagtaaa aacaaacaaa
    14221 gcagaattga attgtttacg acagatggac ttcaagtaat cggtgatatt tcgacgatat
    14281 ctaagaaaat gaaatattat ccgcagatgt cacaatcatt atcaagggat agttcgggta
    14341 aactaaaaac acgaggctat attgatttat cagtcggtgc ttcatttatc ccataccgtg
    14401 gaaacacgtc tagtcaatca gaaagcgata aaaatgtgac taaatcatct caagaggaaa
    14461 atcaagcaaa agaagaatta caaagcgttt taaacaaaat taacaaacaa tcaagtaaga
    14521 ataattaaaa aattttgata ttgtctatgt ttatagttca caagccattc aacgtattgt
    14581 aaactaagga tagtgtattt ttttaatagt aatttgtcag gaggtgccta tctatggaag
    14641 aacattacta cgtaagtatt gatattggat catcaagcgt aaaaacaata gtaggcgaga
    14701 aatttcacaa tggtataaat gtgataggta caggacaaac ctacacgagc ggtataaaaa
    14761 atggtttaat tgatgatttt gatattgcgc gacaagcaat caaagacaca attaaaaagg
    14821 catcaatcgc ttcgggtgtt gatattaaag aagttttcct gaaattacct atcattggaa
    14881 cggaagttta tgatgaatca aatgaaatcg acttttatga ggatacagaa atcaacggtt
    14941 cacatatcga aaaagtatta gaaggtatta gagaaaaaaa tgatgtgcaa gaaacagaag
    15001 taattaatgt gttcccgatt cgttttatag tcgataaaga aaatgaggtt tcagacccta
    15061 aagaattaat tgccagacat tcattaaagg ttgaagcagg cgtaattgct attcaaaaat
    15121 cgattttaat taatatgatt aaatgcgtag aagcatgtgg tgttgatgta ttagatgttt
    15181 actctgatgc atataactat ggttcaatcc taacagctac tgaaaaagag ttaggtgcat
    15241 gtgtcattga tattggtgaa gacgttacgc aagttgcttt ttatgaacgc ggtgaattag
    15301 tagatgctga ttctatcgaa atggcagggc gtgatattac agacgatatt gcacaaggat
    15361 taaacacttc ttatgaaact gctgaaaaag ttaaacacca atatggtcat gcattctatg
    15421 attctgcttc agatcaagat atcttcactg ttgaacaggt tgatagtgat gaaacagtac
    15481 agtatactca aaaagatttg agtgacttta ttgaagcgcg tgtagaagaa atattcttcg
    15541 aagtatttga tgttttacaa gatttaggat taacaaaagt aaatggtggg tttattgtaa
    15601 ctggtggatc tgcaaactta cttggcgtaa aagaattatt atcagatatg gtaagtgaaa
    15661 aagttagaat tcacacgcca tcacaaatgg gaattagaaa acctgaattt tcttcagcaa
    15721 tttctacaat ttctagtagt atcgcttttg atgagttatt agattatgtt acaattaatt
    15781 atcatgataa tgaagaaact gaagaagatg ttattgatgt gaaagacaaa gataacgaat
    15841 ctaaattagg cggatttgat tggtttaaac gtaaaacaaa caaaaaagat actcatgaaa
    15901 atgaagtaga gtcaacagat gaagaaattt atcaatcaga agataatcat caggaacata
    15961 aacagaatca tgaacatgtt caagacaaag ataaagataa agaagaaagt aaattcaaaa
    16021 aactaatgaa atctctattt gaatgattat tggccaataa aactaggagg aaatttaaat
    16081 gttagaattt gaacaaggat ttaatcattt agcgacttta aaggtcattg gtgtaggtgg
    16141 tggcggtaac aacgccgtaa accgaatgat tgaccacgga atgaataatg ttgaatttat
    16201 cgctatcaac acagacggtc aagctttaaa cttatctaaa gctgaatcta aaatccaaat
    16261 cggtgaaaaa ttaacacgtg gtttaggagc aggagctaat cctgaaatcg gtaaaaaagc
    16321 tgcagaggaa tctcgtgaac aaattgaaga tgcaatccaa ggtgcagaca tggtatttgt
    16381 tacttctggt atgggtggcg gaactggtac tggtgcagca ccagtcgttg ctaaaattgc
    16441 aaaagaaatg ggcgcattaa ctgttggtgt tgtaactcgt ccatttagtt ttgaaggacg
    16501 taaacgtcaa actcaagctg ctgctggagt agaagctatg aaagctgcag tagatacatt
    16561 aatcgttata ccaaatgacc gtttattaga tatcgttgac aaatctacgc caatgatgga
    16621 agcatttaaa gaagctgaca acgtgttacg ccaaggtgta caaggtatct cagacttaat
    16681 cgctgtttct ggtgaagtaa acttagactt tgcagacgtt aagacaatta tgtctaacca
    16741 aggttctgca ttaatgggta ttggtgtttc ttctggtgaa aatagagcgg tagaagctgc
    16801 taaaaaagca atctcttctc cattacttga aacatctatc gttggtgcac aaggtgtgct
    16861 tatgaatatt actggtggcg agtcattgtc attatttgaa gcacaagagg ctgctgatat
    16921 tgtccaagat gctgcagatg aagacgttaa tatgattttc ggtacagtta ttaatcctga
    16981 attacaagat gagattgttg taacagttat tgcaactggt tttgatgaca aaccaacatc
    17041 acatggtcgt aaatctggta gcactggatt cggaacaagc gtaaatactt ctagcaatgc
    17101 aacttctaaa gatgaatcat tcacttcaaa ttcatcaaat gcacaagcaa ctgatagtgt
    17161 aagtgaaaga acacatacaa ctaaagaaga tgatattcct agcttcatta gaaatagaga
    17221 agaaagacgt tcaagaagaa caagacgtta atcggttaat atatatacac aaataattca
    17281 acacaaatca tcagataaca tatctgatga tttttttact aatttttaga acatgtagaa
    17341 ggacatttaa gtttttcaaa gttattaaaa gtgtttaagt atcgtgtgaa aattaagtca
    17401 aaaattattt gcgcaacatt ttaactttaa acataaatgt tatattatat aattattaac
    17461 tttgtacagt tagacgaaga taatttaaat gaaatgatgg tgacgatcga gtgaatgata
    17521 attttaaaaa gcaaccgcat catttaatat atgaagagtt attacaacaa ggtattactc
    17581 taggtattac aactagagga gatggtttaa gtgactatcc taaaaatgct tttaatatgg
    17641 cgagatatat tgatgatcgc ccatataata ttactcaaca tcaattgcaa ttagctgaag
    17701 aaattgcgtt tgatagaaaa aattgggtgt ttcccattca aacacatgaa aataaagtcg
    17761 cttgtattac aaaggatgat ataggcacaa atatagacac tttaactgat gcgcttcatg
    17821 gtattgatgc gatgtacaca tatgatagta atgtcttatt aacgatgtgt tatgcagact
    17881 gtgtaccagt atatttttat agtacaaaac atcattttat tgcattggcg catgcaggtt
    17941 ggcgtggtac ctatactgaa attgtaaaag aagtgctaaa acatgtgaac tttgatttga
    18001 aagacttaca tgtcgttatt ggaccatcta catcatcaag ttatgaaatt aatgatgata
    18061 ttaaaaataa atttgaaaca ttgccaattg atagtgccaa ctatattgaa actagaggac
    18121 gagatcgtca tggtattgat ttgaaaaaag ccaatgctgc attattaatt tattatggtg
    18181 ttcctaaaga aaatatttat acgacagcgt atgctacatc tgaacattta gaattatttt
    18241 tctcttatcg attagaaaaa ggtcaaacag gacgcatgtt agcattcatt ggtcaacagt
    18301 aaacaaggag gagatatgtt tgcgtgtgaa agataattta caacaaatct caacacaaat
    18361 taatgacaaa agtgaaaaaa ataatttttc aacaaaacca aacgtgattg cagttacaaa
    18421 atatgttaca atagagcgag ctaaagaagc gtatgaggct ggaataagac attttggtga
    18481 gaatagattg gaaggctttt ttcaaaagaa agaagcatta ccatcagatg cggtgatcca
    18541 ttttatagga tcattacaat ctcgaaaagt taaggacgtt ataaacgacg tagattattt
    18601 ccatgcttta gatcgattga gcttagccaa agaaattaac aaacgtgcag aacataaaat
    18661 taaatgtttc ttgcaagtga acgtttcggg agaagcttct aaacatggta ttgctttaga
    18721 agatgttgat cagtttatag atgatcttaa aaaatatgac aaaatcgaaa ttgtaggttt
    18781 aatgacgatg gcaccattga cagatgatga agcatatatt agatcgttat ttaaacagtt
    18841 acgtttgaaa aaagaagaaa tacaacgact caatttagaa tatgcgcctt gtgatgaatt
    18901 atcaatggga atgagtaatg actatcttat tgcagttgaa gaaggtgcga cgtttgttag
    18961 aattgggact aaacttgtag gagaagagga gtgagccact tggctttaaa agatttattt
    19021 agtggatttt ttgtaataga tgatgaagag gaagtagaag tacctgacaa acaacaacag
    19081 gtaaatgaag cgccagcaaa agagcagtca caacaaacaa caaaacaaaa cgcaatcaaa
    19141 tcagtccctc aaaaatctgc atcaagatat acaacaacgt cagaagaaag gaataaccgt
    19201 atgtctaatt attcaaaaaa taattcacgt aatgttgtaa ctatgaacaa tgctacacca
    19261 aacaatgcat cacaagaaag ttcaaaaatg tgtttattcg aaccacgtgt tttttcagat
    19321 acacaagata ttgctgatga gcttaaaaac cgccgtgcga cacttgtcaa tttacaacgt
    19381 attgataaag tatcagcgaa aagaattatt gattttttaa gcggtactgt ttatgcaatc
    19441 ggtggagata tccaacgtgt aggtactgat attttcttat gtacgcctga taatgtggaa
    19501 gtagctggaa gcattacaga ccatattgaa aatatggaac attcattcga ctaaggagtt
    19561 aacatatgga tataaatgtg ctagctacaa tatttaaatt catccttttt gttgttgaaa
    19621 tttattattt cggcatgatt atatatttct ttacatcttg ggtaccaagt attagagaaa
    19681 ctaaggtagg ttatttttta gcgaaaatat atgaaccttt cttacaacca tttagaaaag
    19741 taattccacc tattggaatt atcgacatat catcaatcgc tgcaattatc gttttagtat
    19801 tattccaaaa agggttactc caaatcttta attggatttt aattcaatta caataaatta
    19861 agcgacataa cgaggcaagg gtacatgata ctttagcctc gtttttgata tgcatttttc
    19921 tgaatataag ggcaatagat ggtattttat aattttttta aggtagtgat taacatagat
    19981 atttatcaac actttagaca ggaagaatac gaattaattg atcagctaac ggataaatgt
    20041 gatcaagcgg aacagcatta tgcaccagta ttaacgcatt ttttagatcc aagagggcaa
    20101 tatatattgg aagtgatttg tggcagttat gaagatttaa acgtatcttt ttatggtgga
    20161 cctaatgctg aaagaaaaag agcaatcatt tcgccgaact attatgaacc taaagaaagc
    20221 gactttgaat taactttaat ggaaatagat tatcctgaaa aattcgtcac tttaaaacat
    20281 caacatattt tagggacatt aatgtcttta ggtatcgaac gcgaacaagt tggagatata
    20341 attgtgaatg aacgaattca atttgttttg acaagtagat tggaatcatt tattatgtta
    20401 gaattacaac gtattaaagg cgcatcagtt aaactttata ctattccagt aacagatatg
    20461 atacaatcta atgagaattg gaaaaatgaa agtgcaacag ttagttcttt aaggttagat
    20521 gttgttatta aagaaatgat acgtaaatca cgtacgattg cgaaacaact aatcgaaaaa
    20581 aaacgtgtta aagtgaatca cactattgtt gattcagcag attttcaatt acaagcaaat
    20641 gatttaatat ccatccaagg ttttggtaga gcacacatta ctgacttagg tggtaaaact
    20701 aaaaaagata aaacgcacat tacctataga acattattca aatagtaatg atttaaggag
    20761 gataacaaat gccttttaca ccaaatgaaa ttaagaataa agagttttca cgtgtaaaga
    20821 atggtttaga acctactgaa gttgctaatt ttttggagca actaagcact gaaattgaac
    20881 gtcttaaaga agataaaaaa caacttgaaa aagtaatcga agagagagat actaatatta
    20941 agtcttatca agacgtgcat caatctgtaa gtgatgcttt gatacaagct caaaaagctg
    21001 gtgaagaaac taagcaagct gcagagaaac aagctgaagc gattatagct aaggcagaag
    21061 cgcaagctaa tcaaatggtt ggtgacgcgg tagaaaaagc acgccgttta gcattccaga
    21121 ctgaagatat gaaacgtcaa tcaaaagtat ttagatcgcg tttccgtatg ttagttgaag
    21181 cgcaattaga cttattaaaa aacgaagatt gggattactt gttgaattat gatttagacg
    21241 ctgaacaagt gacgcttgaa aatattcatc atttgcatga aaatgattta aagccagatg
    21301 aagttgcagc aaatgcacaa aataatgcat caaatacacc agacaataat caacaatcca
    21361 atgattcaga aacaactaag aagtaagaat taaataaaga cagacgcgta atatacattt
    21421 aacttttcac agcgaattag gtaatggtga gagcctagta aaagcatgta tgttatatca
    21481 ctggcttttt aatatttaaa taatgtaatg agagaactct aagttgagtt aataagggtg
    21541 gtaccgcgag caatcgtccc ttttaattta acttagagtt ttttaaattt ttaaggagtg
    21601 aaaaaaatgg attacaaaga aacgttatta atgcctaaaa cagatttccc aatgcgaggt
    21661 ggtttaccaa acaaggaacc gcaaattcaa gaaaaatggg atgcagaaga tcaataccat
    21721 aaagcgttag aaaaaaataa aggtaacgaa acattcattt tacatgatgg cccaccatac
    21781 gcgaatggta acttacatat gggacatgcc ttgaacaaaa ttttaaaaga ctttattgta
    21841 cgttataaaa ctatgcaagg gttctatgca ccatacgtac caggttggga tacacatggt
    21901 ttaccaattg aacaagcatt aacgaaaaaa ggtgttgacc gaaagaaaat gtcaacagct
    21961 gaattccgtg agaaatgtaa agaatttgct ttagaacaaa ttgaattaca gaaaaaagat
    22021 tttagacgtt taggtgttcg tggtgacttt aatgatccat atattacatt aaaacctgaa
    22081 tacgaagctg cacaaattcg tatttttgga gaaatggcag ataaaggttt aatttataaa
    22141 ggtaaaaagc cagtttattg gtctccttca agtgagtctt cattagcaga agcagaaatt
    22201 gaatatcacg ataaacgttc agcatcaatt tacgttgcat ttgacgttaa agatgacaaa
    22261 ggtgtcgttg atgcagatgc taaatttatt atctggacaa caacgccatg gacaattcca
    22321 tcaaatgttg cgattaccgt tcatcctgaa ttaaaatatg gtcaatacaa tgtaaatggc
    22381 gaaaaatata ttattgcaga agccttgtct gacgctgtag cagaagcact ggattgggat
    22441 aaagcatcaa tcaaattaga aaaagaatac acaggtaaag aattagagta tgttgtagca
    22501 caacatccat tcttagacag agaatcgtta gtgattaatg gtgatcatgt tactacagat
    22561 gctggtacag gttgtgtaca tacagcacca ggtcacgggg aagatgacta tattgttggt
    22621 caaaaatatg aattgccagt aattagtcca atcgatgata aaggtgtatt tactgaagaa
    22681 ggcggccaat ttgaagggat gttctatgat aaagctaata aagccgttac tgatttatta
    22741 acagaaaaag gtgcactatt aaaattagac tttattacac atagctatcc acacgactgg
    22801 agaacaaaaa aacctgtaat cttccgtgct acaccacaat ggtttgcctc aatcagtaaa
    22861 gtaagacaag atattttaga tgcaatcgaa aatacaaact tcaaagtaaa ttggggtaaa
    22921 acacgtattt acaatatggt tcgtgaccgt ggcgaatggg ttatttctcg tcaacgtgtg
    22981 tggggtgtac cgttaccagt attttatgct gaaaatggcg aaattatcat gacgaaagaa
    23041 acagtgaatc atgttgctga tttatttgca gaacacggtt caaatatttg gtttgaaaga
    23101 gaagcgaaag acttactacc agaaggattt acacatccag gcagccctaa cggtacattt
    23161 actaaagaaa cagacattat ggacgtttgg tttgattctg gttcatcaca ccgtggcgtg
    23221 ttggaaacaa gaccggaatt aagtttccca gcggatatgt atttagaagg tagtgaccaa
    23281 tatcgtggtt ggttcaactc ttctatcaca acttcagttg ctacaagagg agtatcacct
    23341 tataaattct tactttctca tggttttgtt atggacggtg aaggtaagaa aatgagtaaa
    23401 tctttaggta atgtgattgt acctgaccaa gtggttaaac aaaaaggtgc tgatattgcg
    23461 agactttggg taagtagtac ggactattta gctgatgtta gaatttctga tgaaatttta
    23521 aaacaaacat ctgatgttta tcgtaaaatc agaaatacat taagatttat gttaggtaac
    23581 attaacgatt tcaatcctga cacagatagc attcctgaat cagagttatt agaagtggat
    23641 cgttacttgc taaatcgttt acgtgaattt actgcaagta cgattaacaa ctatgaaaac
    23701 tttgactact taaatattta tcaagaagtt caaaacttta tcaatgttga gttaagtaat
    23761 ttctatttgg attacggtaa agatatttta tatattgaac aacgtgattc tcatatccgt
    23821 cgtagtatgc aaacagtgtt atatcaaatt ttagttgata tgacgaagtt gttagcacca
    23881 atcttagtgc atacagctga agaagtttgg tctcatacac cacatgttaa agaagaaagt
    23941 gttcacttag cagacatgcc taaagttgta gaagtagatc aagctttatt ggataaatgg
    24001 cgtacattta tgaatttacg tgatgatgtg aaccgtgcat tagaaactgc tcgtaatgaa
    24061 aaagttattg gtaaatcatt agaagctaaa gttacgattg ctagtaacga taaatttaat
    24121 gcatctgaat tcttaacttc atttgatgca ttacatcaat tatttatcgt gtcacaagtt
    24181 aaagttgtag ataagttaga cgatcaggca acagcttatg aacatggtga tattgtcatc
    24241 gaacatgcag atggtgaaaa atgtgaaaga tgttggaact attcagagga tcttggtgct
    24301 gttgatgaat tgacgcatct atgtccacga tgccaacaag ttgtaaaatc acttgtataa
    24361 ttgaaattgt ataaagtact catacagatg atataaatta aagctctctt cataatcatg
    24421 ttgtagtttt tgttgacatg atgaagagag tttttttgtg aataaaaaaa tgaccaagtt
    24481 accggtcata tatgtaaaaa atgtgcgatt tactaaaata aaaattattc aggaatggta
    24541 caaattctct gaggcatata aatgcgttat agttgctatt ctcaattatg ttcgcgataa
    24601 ttttaagtaa aagtaagcac agatattgaa tttgatagga gttaattgaa tgtatcataa
    24661 cagtaacgca aactttgtca atggtatcac tttaaatgtg agagataaga atgaattaaa
    24721 gccattttat gaggacatat taggattaaa tattataaat gagacattaa catcgataca
    24781 atatgaagta ggtcaaaata atcatgtcat tacacttgtt gaattacaaa atggacgtga
    24841 acctttaatg tccgaagcgg gactgtttca tatcgcaatt aaactacctc aaattagtga
    24901 tttagctaat ttactaattc atttaagcga atatgatatt ccagttaacg gaggtataca
    24961 gcctgcttcg ttatcattat tttttgaaga cccggaagga aacggtttta aattttatgt
    25021 tgataaagac gaagcgcaat ggacgaggca aaataattta gtaaaaattg atattagacc
    25081 attaaatgta ccgagattag tgagtcatgc aacaaaattg ttatggttag gtattccaga
    25141 tgacgctatt ataggtgcat tgcatattaa gacaattcat ttatcagagg taaaagagta
    25201 ctacctcgat tattttggat tagagcaatc ggcatatatg gatgattatt caatattttt
    25261 agcatcgaat ggctattatc aacatttggc catgaatgat tgggtatcag caacgaaacg
    25321 tgtagaaaat tttgatacgt atggattagc aattgttgac tttcattatc ctgaaacaac
    25381 acatttaaat ttacaaggtc cggatggtat ctattatcgc tttaatcata tcgaagttga
    25441 agattagtat atactttgaa tggacgaacc atataatgaa tcgtttttaa tgatcttttt
    25501 atacaagtta tgaaggaggc tgggacatta agttcttagg caatg
//
LOCUS       DALAGF010000031        24755 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.031, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000031 DALAGF010000000
VERSION     DALAGF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24755
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.031"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            3..380
                     /locus_tag="O2692_001175"
     CDS             3..380
                     /locus_tag="O2692_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002453579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298810.1"
                     /translation="MQLLIATVIISLISIKAFPLAIGALYLPVIFKVIKLQLNLSKGL
                     IDDVNAQTFIKSNQSGIVISVICCLLITAILYYTLDGFYASLTGVLGTLVALNPYTTI
                     VSAVLYILTAIATVEATKTKYRN"
     gene            576..1961
                     /gene="fumC"
                     /locus_tag="O2692_001176"
     CDS             576..1961
                     /gene="fumC"
                     /locus_tag="O2692_001176"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCZ4298811.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAAIANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(2424..3245)
                     /locus_tag="O2692_001177"
     CDS             complement(2424..3245)
                     /locus_tag="O2692_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCZ4298812.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            3408..4520
                     /locus_tag="O2692_001178"
     CDS             3408..4520
                     /locus_tag="O2692_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HCZ4298813.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            4542..5165
                     /locus_tag="O2692_001179"
     CDS             4542..5165
                     /locus_tag="O2692_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4298814.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            5521..5985
                     /locus_tag="O2692_001180"
     CDS             5521..5985
                     /locus_tag="O2692_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4298815.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPVFGTSKMREAETYFNRNAKEELVHI"
     gene            6164..7288
                     /locus_tag="O2692_001181"
     CDS             6164..7288
                     /locus_tag="O2692_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCZ4298816.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLNQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            7357..7701
                     /locus_tag="O2692_001182"
     CDS             7357..7701
                     /locus_tag="O2692_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HCZ4298817.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            8084..8182
                     /locus_tag="O2692_001183"
     CDS             8084..8182
                     /locus_tag="O2692_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298818.1"
                     /translation="MPFDSLAVLITTEIAFPTEIFEKYQRTLQIKF"
     gene            8473..9669
                     /locus_tag="O2692_001184"
     CDS             8473..9669
                     /locus_tag="O2692_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCZ4298819.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            9659..12595
                     /locus_tag="O2692_001185"
     CDS             9659..12595
                     /locus_tag="O2692_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCZ4298820.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQSNIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            12592..13533
                     /gene="yhaM"
                     /locus_tag="O2692_001186"
     CDS             12592..13533
                     /gene="yhaM"
                     /locus_tag="O2692_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HCZ4298821.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(13654..14616)
                     /locus_tag="O2692_001187"
     CDS             complement(13654..14616)
                     /locus_tag="O2692_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCZ4298822.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEFVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
     gene            complement(14821..15378)
                     /locus_tag="O2692_001188"
     CDS             complement(14821..15378)
                     /locus_tag="O2692_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCZ4298823.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALNFGISITDLLYLKIIIFS
                     NYGQYIEEHSTGINILKKIKNPYHL"
     gene            complement(16111..16476)
                     /locus_tag="O2692_001189"
     CDS             complement(16111..16476)
                     /locus_tag="O2692_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCZ4298824.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            complement(16618..17040)
                     /locus_tag="O2692_001190"
     CDS             complement(16618..17040)
                     /locus_tag="O2692_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCZ4298825.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            17174..17914
                     /locus_tag="O2692_001191"
     CDS             17174..17914
                     /locus_tag="O2692_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298826.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDETMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            17907..19130
                     /locus_tag="O2692_001192"
     CDS             17907..19130
                     /locus_tag="O2692_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4298827.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFITFALTYYLLLAPKWYIAFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(19254..19757)
                     /gene="traP"
                     /locus_tag="O2692_001193"
     CDS             complement(19254..19757)
                     /gene="traP"
                     /locus_tag="O2692_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HCZ4298828.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPSIYEVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            19920..20030
                     /locus_tag="O2692_001194"
     CDS             19920..20030
                     /locus_tag="O2692_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298829.1"
                     /translation="MIKCQFYDIYLLKIRTNERYDNIDKNDFNLGGLYGA"
     gene            20020..21057
                     /gene="hemE"
                     /locus_tag="O2692_001195"
     CDS             20020..21057
                     /gene="hemE"
                     /locus_tag="O2692_001195"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCZ4298830.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            21115..22038
                     /gene="hemH"
                     /locus_tag="O2692_001196"
     CDS             21115..22038
                     /gene="hemH"
                     /locus_tag="O2692_001196"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCZ4298831.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            22062..23462
                     /locus_tag="O2692_001197"
     CDS             22062..23462
                     /locus_tag="O2692_001197"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HCZ4298832.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGFDLLKKPTQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYNDQLEEVY
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTHDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            23785..24339
                     /locus_tag="O2692_001198"
     CDS             23785..24339
                     /locus_tag="O2692_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HCZ4298833.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
BASE COUNT         8969 a         3478 c         4324 g         7984 t
ORIGIN      
        1 ggattcaatt attgatagca acagttatta taagtttaat atcaattaaa gcttttccat
       61 tggcaattgg ggcattatat ttacctgtta ttttcaaagt aataaaatta caactcaatt
      121 tatcaaaagg attaattgat gatgttaatg cgcaaacatt cattaaaagt aatcaatcag
      181 gtattgtaat cagtgtaatt tgttgtttat taataacagc aatattgtat tatacacttg
      241 atgggtttta tgcgagttta acaggagttt taggtacgct tgttgctcta aatccatata
      301 cgacaattgt tagtgctgta ttatatatac ttacagctat tgctacggtt gaagctacta
      361 aaacaaagta tcgtaattaa acttagttca ataataacta aataatgata gataacacac
      421 taatcgtatg accgtatcac tgtaataaag ggtgaacacg tgcgattagt gtatttttat
      481 ttcaaattat gaaaacgttt taatcataaa ttgctcaatt aaattattta atgatatact
      541 acagttaaat aaacagtgat aaggggagaa attgaatgtc agtaagaatt gaacatgata
      601 cttttggaga aatagaagta cctgcagata aatattgggg tgctcaaaca gaaagaagta
      661 aacgtaattt cccagttggt aaagagcgta tgccaatcga agtagtttat ggttttgcac
      721 aactaaagcg tgcagcagca atagctaatt ttgatttagg aaaattaagc gaggcaaaga
      781 aagatgccat tgtatacgca tgtgatcaaa ttttatcagg tgaattagat gaacacttcc
      841 cactagttgt atggcaaaca ggaagcggta cacaaagtaa tatgaatgtg aacgaagtag
      901 taagttatgt tgctaatatg tatttaaaag atcatcaaag tgatgaaagt atccacccaa
      961 atgatgatgt aaataaatct caaagttcga atgatacatt cccaactgct atgcacgttg
     1021 cattatatca agaggttgaa acaaaattag aacctgcatt aaaactttta agaaatactt
     1081 tgaaagaaaa agaagataaa tttgattcaa ttattaaaat tggtcgtaca catttacaag
     1141 atgcaacgcc gatcaaacta ggacaagaga ttagtggctg gcgttatatg cttgaccgtt
     1201 gtgaaacaat gttatctgaa tctaagaagc acattttaaa tcttgccatc ggtggtacgg
     1261 ctgttggtac tggtattaat gcgcatcctg aatttggtga taaagtggca cattatattt
     1321 cagaaaatac gggttatcca tttgtatctt ctgaaaataa attccacgca cttacagcgc
     1381 atgatgaagt tgttcaattg catggaacat tgaaggcatt agcaggagac ttaatgaaaa
     1441 ttgctaatga tgtgagatgg ttggcttcag ggccacgagc tggtttggca gaaatttcta
     1501 tccctgaaaa tgaaccaggt tcatcaatta tgcctggtaa agttaatcct acacaatgtg
     1561 aaatgttaac aatggttgca gtccaagtaa tgggtaatga tacagttgtt ggcttcgcaa
     1621 gttcacaagg taactttgaa ttgaatgttt ataaaccagt tattatgcat aatacactac
     1681 aatcaattta tcttttagct gatggtatgg aaacatttaa taacaattgt gcagtgggca
     1741 ttgaaccaat cgaagagaat attgataatt atttaaatca atcattaatg ttagttactg
     1801 cattaaatcc acatattggt tatgaaaaag cagctcaaat tgctaagaaa gcccataaag
     1861 aaggtttaac tttaaaagaa tctgcaattc aaactggata tgttacagaa gaacaatttg
     1921 aagcatggat taaaccagaa gatatggtag atcctcatta attaaatatt aatataaaat
     1981 aaagtttggg cattacaggt agatgtagtg ttcaagcttt attttttgtc tatgtcaaat
     2041 tgaacttata agtctgattt gatatagagc catgaaaagg aaaaactttt catggagtcc
     2101 gttttattat agaacctatt ttttatatga tgaattgttg tacatgtatt ggccaaacaa
     2161 cctgtgataa aaatcttatg atgtcatgta ttgtccatag atacacgtaa cgcagttgaa
     2221 aaagactaca agtcattatc caaggatgtt ataaaatgaa taattactaa aatatcaaaa
     2281 tataaaatcc taagcatcgt aaaaaacaaa aaaaccgaac atctgaaaaa attaataatt
     2341 ttccatctat ccgatttaag ttgatacttg atttagttac aatgcggtat tgcatgttta
     2401 aatatcatgc aaaactaatc aaattagtca tccaaagaaa cggaaatgac ttccttagtc
     2461 aagggatgca caaactctat tttgtaactt tcaagttcta attgtcttaa cgttgaatca
     2521 ccatataaag gatcaccaat aacagggtga ccaatttcag ctaaatgcac acgaatttga
     2581 tgagtacgtc cagtatccaa cttgatttca agttggcaca cagcttcttt tatcattttt
     2641 gaagttaaaa tgtgtgtaat tgcacgctgt ccagtaggag acacacgtcg tttattcgaa
     2701 tggaatttat ctttaccaat tggcatatca atcgtttgtg gttttaaagg taaaagtgcc
     2761 ttaacatttg ctttgtatat ccgcgtaata tcattgtctt ctaacatgcg atcaagaatt
     2821 tttttcatta aaggattttt agcaacaatt aataatccta ctgtttcctg gtccagtcga
     2881 tgaattggtt cgacatagtc actatcaata gtgtaaatca catgattcat taaagtattg
     2941 ctttctttta aatcattagg gtgagtctta acacctttag gtttcattac gattgccata
     3001 tcatcgtctt cgtatttaat ttgtgcataa cgataacttg gaacataatt acttttttca
     3061 tcgatggttg gaacaaatac atcgtcgcca gtatgcactt tatccattaa tcgcgcaggt
     3121 ttatcattaa tagtaatatc tttagacata tttaaatgat gtaaatcttt tttaggtacc
     3181 ttaagttgtt ggaaaatatc tcgtaaactt aagtcattaa agttttctgg tattttaaat
     3241 ttcataaaat cctcctatag tctttaatat catatcacaa atcagttctc cgaaatataa
     3301 ctattgagta atcaaggcca ttttatcatg tttacgtcta agatttaaaa atataataac
     3361 tcaatgtata atagttaata cacgtaaaaa tgaattggag cgatttgatg gaacaaagga
     3421 cgcgactagc tttattaaaa gagattgccg aatttttaaa cgaagaaact gaaatgtata
     3481 gcatgaccca aggtgcgtta aaatatttaa ttgagggcag taatttcacg acaggctgga
     3541 tatttttcat caatagcgta ggtgaacatg aacttgtatc acatgtggct ctaccacaat
     3601 ctttgacagc agaccactgt cactatatta aagatgggtc ttgttggtgt gtgaaagcat
     3661 ttaatcaacg taggctaatg aaagcgtcga atatcgtcaa ttgttctcgt attaacttag
     3721 cctctaaagc atttcctagc caaaatgaca atattacaca tcatgcaacg gtaccactta
     3781 aatcgggtca agaacaattc ggcattttaa atgtagcgtc tccaaatact gaaatatata
     3841 gcgatgaaga tttagagttg ctagagtctg tcgcatttca attagggtca gccatcaaac
     3901 gtatttattt aacagaccgt gaaaaagaag cggcaaaaat taatgaacgt aatcgtttag
     3961 ctagagattt acatgattca gtaaatcaaa tgttattttc tgtaaaacta acagcacatg
     4021 ctgcgtatgg catgtcaaac gaatccatcg ctaaacaagc attcaagacg attgaggaaa
     4081 cgagtcaaaa tgccgttaat gaaatgcgtg cattgatttg gcaacttaag ccagttggac
     4141 ttgagcaagg tttaattcat gctttgacag catatagtaa attaatgcat atacaattaa
     4201 atgtaaatgt agaaggttta atcgatttat ctaatgaaat cgaagaaaac atatacagag
     4261 cattacaaga gtgtattaat aatgttaaga aacatgctga tacgaataaa atggatctta
     4321 cattaaaaca aatgaacgat atactatata ttgacgtgat tgattatggt caaggatttg
     4381 agatcgataa tgttcagata gcttcatcac acggtattaa taacataaaa caaagagtta
     4441 aattattaag aggtaaagtg acctttcatt ctcagccaac aaagggtaca caaatacaat
     4501 tcacaattcc tataaaatag cttgatttaa ggagataacc catgaacaaa gtaatattag
     4561 tagatgacca ttatattgtg cgacaaggat tgcgattttt attatccacg attgaaaaca
     4621 tagaagtttt acaagacttt gcagatggag aaacattttt agaatattta aaagagcatg
     4681 agcaccctga tattgtgcta ttagatttag tgatgcctgg catgaatggt attgaaatta
     4741 cggaatatat taaggcacat tatccggata ttaaagtttt ggtattaaca agttatgttg
     4801 atgatgaaca tgtaatttca gcaatcaata aaggtgctga tggttatgaa atgaaagacg
     4861 ttgagcctca gcaattaatt gaaactatta gacgagttat gaacggtgaa aaaatgatac
     4921 atcctaaggc acaagatgta ttcgaaacag ttagccaaaa accacactac acgaataagt
     4981 tgtcaaagag agaaattgaa gtgttacgtg aaatggttaa aggtaaaaca aataaagaga
     5041 ttgcagaaac tttatttgta tctgaaaaaa caattaaaac acatgtcagt catatattta
     5101 gtaaattaca agttagcgat cgtacacaag cagcaattta tgcaatggaa aataagttga
     5161 tttagaatga gcttttaaat atttgtcgaa aaatagacaa tttttaatct gattttgatg
     5221 aattttttat agcgataggg tttattatac agttttcttt gtaaatttga tatcattata
     5281 aaagtgtaag ttttatcaaa tctagtgatt atgcataaaa atttacttca tttactttcg
     5341 cttcattata aagtaaactt aactcttgtt gacttgatca aaatttaata caaatgtgaa
     5401 attgactctt ttcattttat taaaaatata ttaagatgta atctgtgttt caagaaatgt
     5461 gtattattgc aatttcttgt gtccggataa aattccccaa acagaaaggt agataacaga
     5521 atggatagac agagttttac agatttaatt caaacaaaat ttaaaatggt tcgtatagag
     5581 gctggttata cgcaagatac tatggcgcaa acaattggac tttctaaaaa gactttagta
     5641 caaatcgaaa aagagagagt attaccaaac tggacaactt gtatttcaat ttgtgcatta
     5701 ttcagagact ctgaagtatt aaacagtaca tttggctgtg atccattaga aatcgttcaa
     5761 acaatttcaa gaaatcactg tgcatatcca aaccatgcac caacaagtga tatttattgg
     5821 aataacattg agactcgtaa tgggtacatt ttacaaagta acaaagtaag caatatttat
     5881 cgtgtattaa atccagataa ccaaccagtt ttcggtactt ctaaaatgag agaagctgaa
     5941 acatacttta acagaaatgc taaagaagaa ttagtacata tctaggccta tatactttta
     6001 ataaataata aaagcgatgt aacacacctg tttcgagagg tgaggcatcg ctttttgtat
     6061 gtaaactaat aataaattga gcagaaagtg aaatgattaa tgaattaaaa aataaaaata
     6121 gtggtataat gaataactat gttgtcacaa aggagtcaaa attatgaatg cactatttat
     6181 tatcatattt atgattgtag taggggcgat aattggtggc attactaatg taattgcaat
     6241 tagaatgctg tttcacccct ttaaaccata ctatatattt aaatttagag tcccatttac
     6301 accaggttta ataccgaaaa gacgcgaaga aattgcaact aaaattggcc aagtgattga
     6361 agagcatttg cttacagaaa ctttaattaa tgaaaaattg aaaagcgagc aatcacagca
     6421 agcaatagaa tctatgattc aacagcagtt acaaaagttg acgaaagatc aattgtcaat
     6481 aaaacaaatt acttctcaaa tcgatattga tttagaacaa gtattacaaa ctaatggaaa
     6541 tcaatatatt gaatcacaat tgaataatta ttatacaaag catcaaaacc aaacaatagc
     6601 atctttattg ccaaatcaac ttgtaacatt tttaaatcag catgtagata acgcaacaga
     6661 cttattatgt gatcgtgcaa ggaattattt atcatctgca aaaggcacac aagatattaa
     6721 tgatatgttg gatacttttt tcaatgagaa aggtaagtta attggtatgt tgcaaatgtt
     6781 tatgacaaaa gagagtattg cagatcgcat tcaacaagaa cttatacgtt taacatctca
     6841 tcctaaagca agaacaattg tgacatcgtt aattaccaat gaatatcaaa cttttaaaga
     6901 taaaccattg aatgaactgt tagatgcatc gcagtttaat gaaatcgctg aaaatctatc
     6961 agtgtatgta acaacatatg caagcaaaca agcgaataag ccagtggtta cgttaatgcc
     7021 tcaattcgtt gattatttag aaggtcaact ctctagcaaa ttagcaaatt taatcattga
     7081 aaaattatcg attcacttat ctacaattat gaaaaaagtg gatttacgag gattaattga
     7141 agaacaaatt aatacgtttg acttagatta tattgaaaaa ttaattatag aaattgctaa
     7201 caaagaacta aagttgatta tgtcattagg tttcatttta ggtggtatta ttggattttt
     7261 ccaaggtttg gttgcaatct ttgtctaaaa aagcgagatg tggtaaagta atatcgaacg
     7321 ttttaataaa catcacaaca aaggagtgct ataaccatgg cagtaaattt atatgattat
     7381 gcaaatcaat tagaacaagc tttaagagaa agcgaagaat acaaagcaat caaagaagca
     7441 ttcgctaatg taaaagctaa cgaagaatct aaaaagttat tcgacgagtt ccgtgaaact
     7501 caaattaact tccaacaaaa acaaatgcaa ggtgaagaaa ttgctgaaga agatttacaa
     7561 aaagcgcaag aacaagcgca agcaattgaa aaagatgaaa acatctctgc attaatgaat
     7621 gctgaacaaa aaatgagtca agtattccaa gaaatcaacc aaattatcgt taaaccatta
     7681 gacgaaattt acgctgacta atattaaaat aatatttttc aatcctttaa atagaggaat
     7741 agactgccga cagagtccga gactttgtgg gtagtttttt tagttttgat aacggaagtt
     7801 agaggctctc tgtcaaattg ggcagatgca ttcagtaacg gaagttaggc tagtaaacgt
     7861 gtcctaactt cctttttgca ttttgaagtg taaagttttc aacataatac tattagttcg
     7921 gtcatgtatc ggactgatgg aaaagcgttt cacttttaat gactcattaa gaacggcctg
     7981 aaaatgtttg gcgtattaag tgcaatgata gttttgacat ttagtttcta attggtcatt
     8041 actgccgagc aaatctagta gagtaatcat gtaaatcttt aatgtgccat ttgattcact
     8101 agcggtgtta ataactacgg aaattgcatt tccgactgaa atttttgaaa aatatcaacg
     8161 tacgctacaa ataaaatttt aaactgttat aaatgtgtct caatttcata tgttcatcga
     8221 cgatatgaag cgtattatgg taaaatgaag aaataataaa cttgttaata aataaaacat
     8281 cacgatttga ctaaagcact ttattattgt gtagataata gttttttaac gaaataaaaa
     8341 tggcgactgg ttttaataaa tcagctaatg aatcactaca cctataagta tgaatatagt
     8401 gattagaatg ctttgtatag ttggattttg caaaattgat gttagaacct ttaattaagg
     8461 agatatggat gtatggttaa atttattcat tgttcggatt tacatttaga tagtcctttc
     8521 aaatctaaga gtcatataag ccctaaaatt tttgaggatg ttcaaaaaag tgcttatgaa
     8581 agttttaaaa atattgtaga tattgcatta caacaagatg ttgattttgt aattatcgcg
     8641 ggtgatttat ttgatagtga aaacagaaca ttacgtgctg aaattttctt aaaacagcaa
     8701 tttgaacgtt tacaaaatga acaaatcttt gtttatgttt gtcatggtaa tcacgatcct
     8761 ttatcatcaa agatttcatc aaactggcca gataatgttt ctgtattttc aaataaagtt
     8821 gagacgtatg aagcaattac taaatctggt gaaacaattt atattcacgg atttagttat
     8881 gaaaatagag caagttatga gaacaagatt gatgaatatc catcaagtca aggccaaaaa
     8941 ggcatacata ttggtgtctt gcatggtacg tatagtaaat cttcagttaa cgaaagatat
     9001 accgagttca ttttagaaga tttaaacagt aaattgtatc attattgggc tttaggtcat
     9061 atacatgaac gtcaacaatt aagtgatatg cctgtaatta actattcagg taatattcaa
     9121 ggtagacatt ttaatgagca aggtgaaaaa ggttgcttat taatcgaggg tgaccactta
     9181 aaattaaaga ctaaatttta tcctacacag tatattagat ttgaagaagc aactattgaa
     9241 acggataaga catctaagca aggtttatac gaggtcattc aaaactttaa agaacaagtg
     9301 agagaagaag gaaaagcctt ttatcgttta acgcttgtta ttaatagtga gacattaatt
     9361 tcacctcaag atttattaca agttgaagaa atgattacag attatgaaga aaacgaaaat
     9421 caatttgtat atattgatga gttaaaaata caatatgcac aaaatgatga gtcaccttta
     9481 gttaatgaat tttcagcgga attattagtc gatcaaactg tttttgataa agcgatgtca
     9541 gatttatatt taaatccaag ggcatctaag ttcctagacg attatggaac attcgaccat
     9601 acagcattag ttaatcgtgc tgaagaaata ttaaaagctg aaatgagagg tgaacaaaat
     9661 gataattaaa tcacttgaaa tttatggtta cggtcaattt gttcaacgta aaattgaatt
     9721 taataaaaac ttcactgaaa tttttggtga aaatgaagcg ggtaaatcga cgattcaagc
     9781 attcatccat tcgatattat ttggatttcc aactaaaaag tctaaagagc caagactaga
     9841 accacgtcta ggtaaccaat acggtggtaa attagtactt attcttgatg atggcttaga
     9901 gattgaagtt gaacgaatta aaggcagtgc tcaaggtgat gtgaaagtat atttacctaa
     9961 tggtgctgtg cgtgatgatg cttggttaca aaagaaactt aattatattt ctaaaaagac
    10021 atatcaaggt atcttttcat ttgatgtact agggcttcaa gacattcata gaaatctaaa
    10081 tgaaaaacaa ttgcaagatt atttattaca agcaggggct ttaggatcaa ctgaattcac
    10141 gtcaatgcgc gaagtgatta atcgtaaaaa agatgaatta tataaaaaat caggtaaaaa
    10201 tccgatcatt aatcaacaaa ttgagcaatt aaaacaacta gaaagtcaaa ttcgtgaaga
    10261 agaagcaaag ctagaaacat atcatcgctt agtagatgat cgagataaat catcacgtcg
    10321 attagagaat ttaaagcata atttaaatca attatcaaaa atgcatgaag aaaaacaaaa
    10381 agaggttgct ttacatgatc attcacaaga atggaagtct ctagaacaac agttaaatat
    10441 tgagccaatc acattcccag aaaaaggtgt ggatcgttac gaaaaagcac gagcgcataa
    10501 gcaatcgtta gaaagagata ttggtttaag aaatgagcgt ttagctcaac ttaaagaaga
    10561 agcgactcaa ttagagccag ttaaacaatc tgatattgac gccttcatta gtttgaatca
    10621 acaagaaaat gaaattaaaa ataaagaatt tgaacttact gcaatcgaaa aggatattgc
    10681 gaataaacaa cgtgataaag atgaattgca atcaaatatt ggttggtctg aaacgcatca
    10741 tgacgtagat agttcagagg caatgaaaag ttatgtcagt gagcaaatca agaataaaca
    10801 agaacaagct gcatacatta aacaattaga acgtagttta gaagaaaata aaatcgaaga
    10861 taatgcggtt catagcgaac tagattctgt tgaagaaaaa atagttcctg aagagacttt
    10921 tgaaaagaaa aaagaatact cacaacaagt cattgaatta aacgaaaaag agaacttgta
    10981 tagcaaattg aaagaacgtt tcgaaattga gcaacaagaa aaacaaaaac gtcaaaaatt
    11041 gttacgaaca acgtttattc ttttaacatt agtaggtatt ggtttaactg ccttctcatt
    11101 tatttcaaac aacatgttgt ttggtattat tttcgcagta ttaactttag tatttgttat
    11161 cggtatcatc atgtctaaat caaaagaagt agattatagc gaggccatca cagatgaaat
    11221 cgaagaaatt aaagcacaac tagccatttt agatgaaaat tatgacttag attttgatct
    11281 tgatgagcaa tatcgcattc gtgatcactg gcaacaagct ttgaaaaata aagatatttt
    11341 agaagagaaa cgtcagtata ttgaaggtcg tttaaatgat gcgaaagggc gtcatgatga
    11401 attacaatcg acagttgaga acgttaagga tgaattatac ttatcatcta aaatttcaaa
    11461 tgatttaatt gttgatagta tttctacaat ggcgaatatt aaagcattag atcaacatat
    11521 cagtgattta aatcaacaac gccagcaatt ggttcaagaa ttagatacat tctacaatca
    11581 tgctgaagct gttacaaaat cacaatttgt ttatttcaac aaattatctt tattccacga
    11641 tgtgcaacaa tggttgaaat cagctgaaga cacgaatgaa aaatggcgta ttaatgctga
    11701 aaataccaaa cttgttacaa atgaattaaa tcatttaaat gctcaattag aagaaaataa
    11761 taaagaaatt acagcactat tcgattttat caatgttggt actgaagaag atttttacca
    11821 acatcatgaa gattaccaaa cttatactag taatttgagt cgttttaatg atttaactaa
    11881 atatcttgag aaccaaaact attcttatga attaagttct agtttaagtg aaaaaacaac
    11941 tgcacaactt gaagaagaag atcatttatt ggctactcaa gttgacgaat ataatgagca
    12001 atatcttgaa atgcaagcac aagtcagtga tttaagtgca caaatcaatc acatggaaac
    12061 tgatacaacg cttgctaatt taagacatga atatcacagt cttaaaaatc aacttaatga
    12121 tatcgcaaaa gattgggcaa gtttaagtta tttacaaagt ttagttgatg aacacattaa
    12181 acaaattaaa gataaacgtt tgcctcaagt tattaatgaa gcggtagaaa tattgaagca
    12241 tttaacagat ggcagatata cgatgattaa ctataatgaa gattcaatta cggttaaaca
    12301 tgttaatggt caattatatg atcctgttga actaagtcaa tctacaaaag aattacttta
    12361 tgtagcttta cgtatcagtt taattaaggt actaagacca tattatccgt tcccattaat
    12421 tgttgatgat gcatttgttc attttgataa aaaacgtact gaaaaaatgt tgaattattt
    12481 acgatcatta tcagaacact atcaagtact atactttaca tgtgtaaaag ataatattgt
    12541 tccatcaaaa gaagtgatta cattaaacaa aatagaggaa ggcgggaaac gatgagaaat
    12601 atagagaatc taaatcccgg agattcagtt gatcactttt tcttagtgca taaagctaca
    12661 cagggtgtaa cagcacaagg taaagattat atgacattac atttgcaaga taaaagtggt
    12721 gaaattgaag cgaaattttg gacggctaca aaaaatgata tggcaacaat caagcctgaa
    12781 gaaattgtac atgttaaagg tgacatcata aactatcgcg gaaataaaca gatgaaagtc
    12841 aaccaaatta gactagcgac aactgaagat caattaaaaa cagaacaatt tgtagatggt
    12901 gcacctttat caccggcaga aatacaagaa gagatttctc attatttgct agatattgaa
    12961 aatgctaatt tacaacgtat cacacgtcat ttattgaaaa aatatcaaga acgattttac
    13021 acatatccag ctgctagttc tcatcatcat aactttgcga gtggcttaag ctatcatgta
    13081 ttaacgatgt tacgtattgc aaaatcaatt tgtgacattt atccattgtt aaacaaaagt
    13141 ttgttatata gtggtattat tttgcatgat attggtaaag ttagagaatt gagtggtcct
    13201 gttgcgacgt cgtatacagt cgaaggtaac ttattaggac acatctcgat tgcgagtgat
    13261 gaagtagttg aagcagctcg tgaattgaac attgaaggag aagaaatcat gttgttaaga
    13321 catatgattt tatctcatca tggtaagtta gagtatggtt ctccaaaact gccatactta
    13381 aaagaagcag aaattttatg ctatatcgat aatatcgatg ctagaatgaa tatgtttgaa
    13441 aaggcatata aaaaaactga caagggtcag tttacagata aaatatttgg tcttgaaaat
    13501 cgtagattct acaatcctga atcactcgat taagaattaa aagatatcgg acagatgagt
    13561 atactgaaac atgagctcat ctgtccgatt tttaatgcga aaaaaccgag cggccgtggt
    13621 atgatttttg aaccacggtc gctcggtttt gtgttattgg ctcatgccgg attgtccgcc
    13681 ttgtgcgcca ccttgtttaa gtttttcagg gtttaagatt ttatcttcga caactgattt
    13741 aatatcacga tctttaaagt caacatcgta ttcttttaat agatctttgt atgcatcagt
    13801 caataatttt ggattttttt gtactttctg atcgactaat ttttctttca ggctttgttt
    13861 ttcactgtta aagtctgttg gtttatcagc tttaataata tgatatccaa agcttgattt
    13921 aacaaactct gatacttcac catctttaag cttaaatagt gctttttcaa aatctttatc
    13981 agtttgtcct ttaagaacat aacctaattc gccatctttt ttagctgaac cagtatccat
    14041 tgattctttt ttagcgattt caccaaattt acttggatct tttgaaactt ctttttgaat
    14101 ttcttcagct ttttgtttcg cttctttatc atctaagcct tctttgtcgc ttttcttaga
    14161 tttaacttta attaaaatgt gtgaagcttt cttgctgtct tctttaattt cagaatcaga
    14221 gattttaatt ttatctgata gtaattcttt atgataagca gcagtacgta aattttcttt
    14281 atatttatcg gctgttaaac cttgctgttg aagggccttt tcaaatttat ctttaccgcc
    14341 gtattgcttt tgcatttttt caatttgttc gtcaatcttc ttatcattaa ctttattttt
    14401 atatttatca gctaaaattt tatttaacat ttcagtaaat gatgcatttg caatttgatc
    14461 tttaccgatt tttttcattg tatctgcaac tgttacgtct ccagctttag aagaaattaa
    14521 tgtattttct ttagagtctg tggcactagc gccacaagcg cctaataata aagcactagc
    14581 tgttaccgga acgattaatt tgtttatcat cttcatagtt gaaactcctt tgtaagttaa
    14641 tcatcgtgaa tataacatag ttttatatta tcaccaatca ataacccatg acatcagact
    14701 agagcattag gtttaggggt agaaattgat gaaaataaaa aaacaaggtc tatatacatt
    14761 tgattgtaag ctattacata gcctaaaaac aaataataac accttgctat aattattttg
    14821 ttataaatga tatggatttt taattttttt caaaatatta atacctgtac tatgttcttc
    14881 tatatattgt ccataattag aaaatataat tatttttaaa tataataaat ctgtaatgga
    14941 aataccgaaa ttcaatgcca ataagaacat aaaataatgt ccatattgcg ggaatgaaat
    15001 tgttagataa acgaacattg cagataagat aattaacggc aatattaagt taatgcaaaa
    15061 ataaaatttg tgtacaggtt gattgacgta ggtattatag aatataagcc attttctttt
    15121 agttaactta tgaactttaa acgattttct atatggtaag aaaaataata aatgtatact
    15181 tttatgtaat ggatacgtca taaatacaag taataaaaag atgagaaaat gtctatctga
    15241 taatggcgta ttagataaaa aatacattac ttcataacta actaaaaaca taatgatggt
    15301 tgcaactgca ctcataaatg caattctagg caaaccaaat cgtgcattga tatcaatttg
    15361 tcttttacat aaaaacatat cttacgcctc atctcttttc aatataagtt agattataac
    15421 taactcaggt tcagtaattc aatagtaaca tatcatgtta gaagaattgt tatataagaa
    15481 gaaaggagcg acgtattgtt tgatttgaaa cgcgaaagtg gggattggta aaattgtctg
    15541 attgactagc gtagaagtag aatgcagaac aaatttctgc gaacacatat tcttgctaat
    15601 aaagcatcac ttatgaaaaa ggcgcgtgtg acatgagtat ttgaaaactt gaataatata
    15661 aaaaatggat atggcagggc atatgcaaaa gcatcttttg accaactaag ttatacaaat
    15721 gctaaaaata tagtgatatg taattataat aaacaccaga ttaataattt gtgcaagtaa
    15781 ggtatatgta atgtgtatat aaggttcggc tcaatgaaaa tataagtatg ttgtttgtcg
    15841 ataatgattt aagtaaagcg taatcaaagt gcaaggataa cacaatgaat cctaagcaat
    15901 gttttcaatc aattcataaa tatgatttaa gcgaaaatca ctacaaacaa tgtcttccta
    15961 taatttgtaa acataagttc atttgagttt taaaaaagag atgacaagaa atatctaatt
    16021 tctaatgtca tctcgtttat ttggcattta attttaaaaa ataaatatgc tgttactaat
    16081 aaaaatagtt gtgatcgaga acgtaacctg ctacttagaa atttcgtttc caatatcctc
    16141 gccacgattt tgtaaatttt cgatgtgtga ctgtaaacgt tcaatattag gattaatgtc
    16201 agatttgaag tttccaagta atgacttaat ttcaccacca attgaactac caaattccgc
    16261 actttcgttt ttaatttgaa caccatagtt taaaatgtca ttaaaacttt gtttaatcgt
    16321 ttcaaattca cgttgtagtt ctgattttga accagtaggg gcagtacgat cgatcgtgtt
    16381 gttcttgaca cttttgtcgt cacgtccttg aagtgcaact acaaaaccag ctgctacgcc
    16441 aacaccgata ccgaatagaa tgcgtgatgc tttcatttat attctcctca cttttaacat
    16501 ctgttatctt gataactcat tttgtttaaa attgctaacc ttgatgttat cacttcaaac
    16561 gcaattgtta taatattaat catattatac ccgttcgagc ttagattaag catacattta
    16621 aaattgtgct tgaatttgtt cagcaatttg ttgttttgca tcgttatcta aaatgtcttc
    16681 atgtgtttcc cacttataac caaatccatc aatatcattt tcgtatcgag gaattaagtg
    16741 gaaatgaata tgaaatacag attgatctgc aaactcacca ttattttgaa taatgtttaa
    16801 accatcagga ttaaatgcac gcttaatagc atttgctact ttaggtaatg ctgcaccgat
    16861 atgtttcatt gtttcttcat cagtttcaaa gatattagca gaagcttttt taggaattaa
    16921 taacgtatgt cctttagtaa cttgtgatat atctaaaaag gcatagacat aatcgtcttc
    16981 atatacttta aagctaggaa tttctccagt taaaattttg ccgaaaattg tttctgacat
    17041 gatgactaca ctccttttca actagtatta ccattcatta taacattgtc gctaaaaatt
    17101 cgatattagg acatgtgtat ttaatatgag cattttataa tttttggtac aatataagtc
    17161 atggaggtgc cttatgacag ttaaagtaga acagcttaca ggtggatatg gaaaacgccc
    17221 tgtaattaaa gatataaatt ttgaattaaa caaaggcgaa attgttggac ttattggcct
    17281 taatggcgct ggtaagagta caacgattaa acatatgcta ggattgctta cacctatgga
    17341 aggatctcta tcaatttcag atatcaacat taatgatgac atagaggctt atagaagaaa
    17401 gttatcttat attccggaat caccggttat ttatgaagaa ctcacattag aggaacacat
    17461 tgagatgaca gcaatggcat atgatattga tcgtgatgaa acgatgaatc gagcaatgcc
    17521 attattaaag acattccgtt tagaaaatga gttgaaagtt tttccgagtc atttttctaa
    17581 ggggatgaaa caaaaagtca tgattatttg tgcgtttatc gttaatcccg aattgtatat
    17641 tatagacgaa ccattccttg gcctagatcc attaggaatt caatccatgt tagatttaat
    17701 ggttgaaaag aaaaacgaag gtagaaccgt tctaatgagt acacatattt tagcaacagc
    17761 tgaacgttac tgtgatcgtt ttatcatact agacgaaggt gaagtcgttg catttggaga
    17821 tttagaagca ttgagacaac aaactggctt acataatcaa acattagacg atatatatat
    17881 tcatgtgacg caaggtggag atgtacatgc gtaaccaagc taccacatta tttaataaaa
    17941 gattacatgc ccttcgtaaa gagaaaaatt attataataa gtttatattt aatggtcatt
    18001 tcatggtctt tttacttatt ttgctgggtg catttatttt tggatatggt gaatggttaa
    18061 aacatattcc aacgaatatc aatttttcat taatagcagc agtgattgtt gcgttgacat
    18121 ctatttttcc aatgcgacca ctgttaaaag aagcggataa aatcttttta ttaccatttg
    18181 aaaaacacat gtctcaattt atgcgtcatg cgattttata tagttacttt gcgcgcatat
    18241 taatccaact cattattgtt attgtaatgt tcccgttgtt ttacaatatt aatcaacata
    18301 atgtggcatt ttatatttgt tttggagtca gtgcattaat ttttccatat gttggtttac
    18361 gcttaagatg gcaatggtat caatcgggac ttaaaacatg gcaagttaat ttaatatcat
    18421 tcatcacctt tgctctaaca tattacttac tattagctcc aaaatggtac atcgcatttg
    18481 tgatggttgc tttgcctgtg ttaatagaat ttttagttaa aaaatataaa ccaggctttt
    18541 tgtatccttg ggagaaaatg attgcaatcg aacatcgaca tcatatgaat tattataaat
    18601 ttgttaatat gttcaccgat gtaaaacatt tgaaagaatc tgcagtcaga agaagttatc
    18661 tagatatact tttaccagtt ccaaagggta gtaaatttaa ctctaatgcg atgtatttat
    18721 tcttatttat tagaagcttt ataagaggga gagacgcatt taatattatc tttaggttag
    18781 taattattgc agttttatta atggtttggt tatcatatcc attagtgaca gcaattattg
    18841 ggtgtttatt tgtatatatt atattgttac aaatggcaca attttattca cagcaagcat
    18901 atggcttatg gcctcaagtg tggcctgtac ctgaagagaa ggtaatcaaa gggtatgaac
    18961 agtttttata tagattaatg tttgttattt gtactgtgtt tgcagtgaca tttattataa
    19021 aacatatgac gttattctat gttgtactca ttttctatat tgtaggttta ttaacgatta
    19081 gaagtattat taaaaagttg aaatatcagg aaacattatt acgagactaa aaaacttcca
    19141 ttggcatgta tgttgtaaag gtgcatgtaa tgttgaacgc caaatgatac ggcgttcaga
    19201 ttacattagc atctatacgt taacagcata accaatggaa gttttcttcg aatctattct
    19261 tttattgggt atagatatct ttcaaaataa cttgaatgtt gtccgcttga accaaagtaa
    19321 tgacttaatg cgtcttttga aaaatggtca ttaaaggcat cagattgctt aaagtcttcg
    19381 tatgcatgtc gatcagcaaa tccgaaataa attttgtatg ttgtaccttt agcaggtctt
    19441 aacaaacgat agcttttaaa gccaccaaag tttctgaaat tatcgtctac actaatcagt
    19501 ttcttttcaa gttgatatgc atgatcttct gttgatggaa tgaagattgc acaatagaaa
    19561 tgatgttcac tgaattcacc aatttcttta ataacttcat atattgaagg tgattttaaa
    19621 acagtctcac catcagtttc ttcaaaaata actgaagtat ctgatgctga aaattggaat
    19681 agttgatggg tcgggttatt gatttttatc tgatgtaaaa atccataagt gccataagat
    19741 gtatatagtt tcttcatatg aaaggtcccc cttatgtttt tatttattat acgatgataa
    19801 actagtcatt accactatta ataattgatt aaaattacta taatgaatct tgcgagatat
    19861 ttagaagtaa tgatacaaaa taatggactt tagaatttaa atttacaata aatagttcta
    19921 tgattaaatg tcagttttat gacatttatt tattgaaaat acgaacgaat gagcgatatg
    19981 ataatataga taagaatgat tttaatttag gaggccttta tggtgcataa taaaaacaat
    20041 acaattttaa aaatgatcaa aggtgaagaa acatcacata cacctgtttg gtttatgcga
    20101 caagctggcc gttcgcaacc agaatatcga aaattgaaag aaaaatattc actattcgat
    20161 attacacatc agccggagtt gtgcgcttat gtaacacatt taccagttga taattatcat
    20221 acagatgcag caattttata caaagatatt atgacaccat taaagccaat tggtgtcgat
    20281 gtagaaatta aatcgggtat tggtccagtg attcataatc caatcaaaac aattcaagat
    20341 gttgagaaac tttctcaaat agaccccgaa cgagatgtac catatgtatt agatacaatt
    20401 aaacttttaa cagaagaaaa gttaaatgtg ccgctaatag gatttactgg ggcaccattt
    20461 acattagcgt catatatgat tgaaggcgga ccatcgaaaa attacaattt tacaaaagcg
    20521 atgatgtata gagatgaagc aacatggttt gctttaatga atcatttagt tgatgtatct
    20581 gttaaatatg taacagctca agtcgaagca ggtgccgaat tgattcaaat tttcgattca
    20641 tgggtaggtg cattaaatgt cgaggattac agacgttaca ttaaaccaca tatgattcga
    20701 ttaatcagtg aggttaaaga aaaacatgat gtgccggtaa ttttattcgg tgtaggtgcc
    20761 agtcatttaa tcaatgaatg gaatgattta ccgattgatg tattaggcct agattggaga
    20821 acgtctatta atcaggctca acaattaggc gttactaaaa cattacaagg gaatttagat
    20881 ccatcaattt tattagcacc atggaatgtc attgaagaga gattgaaacc aatattagat
    20941 caaggtatgg agaacggtaa acacatcttt aatttaggac acggtgtatt cccagaagtg
    21001 caaccagaga cgttacgtaa agtaagcgaa ttcgtacaca catatacaca aagataatca
    21061 tagttttaca ttgaagcaca tgaaagataa ttcatttaaa ggaaggtatt atcaatgact
    21121 aaaaaaatgg gattattagt tatggcttat ggcacacctt ataaagaaag tgacatagag
    21181 ccatattata cagatattag acatggtaaa cgtccatctg aagaagaact tcaagatttg
    21241 aaagatagat atgaatttat aggtggttta tcaccattag caggtacaac agatgaccag
    21301 gctgatgcgc tagtttcagc attaaataaa gcatatgcag atgttgaatt taaactatac
    21361 ttaggattaa aacacatttc accatttatc gaagatgcgg ttgaacaaat gcacaatgat
    21421 ggcattactg aagcaatcac ggtagtacta gcaccacatt attcttcatt ttcagtagga
    21481 tcatatgaca aacgtgctga tgaagaagct gcaaaatatg gtattcaact tacacatgtg
    21541 aaacattatt atgaacaacc taaatttatt gaatattgga cgaataaagt caacgaaaca
    21601 ttagctcaaa taccggaaga ggaacataaa gacacggtat tagttgtttc ggcacatagt
    21661 ttgccaaaag gtttaatcga aaagaataat gatccatatc cacaagaact agaacatact
    21721 gcgcttttaa ttaaagaaca atctaatatt gaacatatcg cgattggttg gcaatctgaa
    21781 ggtaatacag gtacaccttg gttagggcca gatgtacaag atttaacacg tgatttatat
    21841 gaaaaacatc agtataaaaa ctttatatat acgccagtag gttttgtatg tgagcattta
    21901 gaggtgcttt atgacaatga ttatgaatgt aaagtagttt gcgatgatat tggtgcgaat
    21961 tattatcgtc caaaaatgcc gaatacacat ccattattta tcggtgcaat tgttgatgaa
    22021 atcaagtcta tattttaatg acgaaagaag cgtgaaacgt tgtgactaaa tcagtggcta
    22081 ttataggagc ggggataaca ggtttatcaa gtgcatattt tttaaaacag caagatccta
    22141 atattgatgt aaccatcttt gaagcatcga atcgtccggg gggaaagatt caatcgtatc
    22201 gtaaagatgg ttatatgatt gaactagggc ctgaatctta tttaggtaga aaaacgatta
    22261 tgacagaatt agcgaaagat attggattag aacaagatat tgttacaaat acgactggac
    22321 aatcatatat ttttgcgaaa aataaattat atccgattcc aggtggttca attatgggta
    22381 ttccaacaga tattaaacca tttgttacta caaaattaat atcgccactt ggtaaattaa
    22441 gagcagggtt tgatttatta aaaaaaccta ctcaaatgca ggatggtgac atttctgttg
    22501 gtgcattttt cagagcaaga ttaggtaatg aggtacttga gaatttaata gagcctttaa
    22561 tgggtggtat ttatggtacc gatattgata aattaagttt gatgagtacg tttcctaatt
    22621 ttaaagaaaa agaagaggca ttcggaagtc tgataaaagg tatgaaggat gagaaaaata
    22681 agcgtctgaa acaaagacaa ttatatcctg gcgcaccgaa aggacaattc aaacaattta
    22741 agcatggttt aagctcattt attgaagcat tagaacaaga tgttaaaaat aaaggtgtga
    22801 caatacgcta caatacgtca gtggatgata taattacatc tcaaaaacaa tataaaattg
    22861 tttacaatga tcaactagaa gaagtgtatg atggcgtatt agtaacgaca ccacatcaag
    22921 tgtttttaaa ttggttcgga caagatccag catttgatta ctttaaaacg atggatagta
    22981 cgactgttgc aactgttgta ttggcatttg atgaaaaaga cattgaaaat acccatgatg
    23041 gtactggctt cgtaattgcg agaacgagtg atacagacat taccgcatgt acttggacat
    23101 cgaaaaaatg gccatttact acaccggaag gtaaggtttt gattcgtgcg tatgtaggta
    23161 aaccaggtga tactgtggtt gatgatcata cagataatga attagtatcg attgtacgta
    23221 gagatttaag tcaaatgatg acatttaaag gtgatcctga atttacaatt gtcaatcgtt
    23281 tgccgaaaag tatgccacag taccatgtcg gtcatattca acaaattaga cagattcaag
    23341 cacatattaa acaaacatat ccacgactta gagtaactgg tgcatctttt gaagcggttg
    23401 gactacctga ttgtattacg caaggtaaag ttgctgctga agaagtaatc gcagagttgt
    23461 aagttaattg ttggatggtg ttggtgtagg tgcgacgcct ttagataatg tttgatgaaa
    23521 tatagtcatt tttaagttat ataatggtgg aagtaatgtg taaatataga tactttcagc
    23581 gggaattatt tttgtgaaat tgaagcagag acaaaggttt agctgttttt gaatgatatt
    23641 gaaatgatga caatacactt taattagaaa tgtcactttg cgatataaca tttctaatta
    23701 aggtgttttt attttacaat tataggtaat cagtcatttt tttaaaaata gaaaagaagt
    23761 agtaagttga aaggagagaa tgagatgaca gatgtaatta ttgtacattc tatgcatgga
    23821 aatagtagaa atcattggta tcagtggttg gagcataact taactttaga aggatatgac
    23881 gtaacattat ttaattttga atcccctgaa gcgaagacag tggatcagtg gattgaagct
    23941 atgacgaaac aaattaatgt acgcaaaaag gatacatatt ttgtaacaca tggtttaggt
    24001 tcaatcacag ctttaaaata tattgaaatg attgatcagc cgattgaagg ctttttcagt
    24061 atagcgggct ttaaagaaga tgcagaaaat atagatttag atattgattt aagtaatgta
    24121 accattgatt acgataatat taaaaagaaa gttgacaatt tttacggatt gagttctaaa
    24181 aatgacaaat acgtttcata taaagagacc cagcgtttaa tgaatgcatt agaggggaac
    24241 ttgcgaatcg tggaagatgg agggcacttc ttagaagagg atggcttcga aacatttaca
    24301 gcgttgcaag atcggatgca agattacatg acacgatagt tagtgtatgg gttgtgttat
    24361 gttgtgtcag taggtagctg ctgattttga aaaattgttg taattgaggc gtcaagcaat
    24421 gtcttagggc tacaatgtta tattggtagt cgctgactga aagaatatgc ggtgtaacaa
    24481 gcttttataa tttctttttc agaaggtata ggcgttgtaa actttataac tcctatacct
    24541 ttttaaatgc tttaacacta tttatgacaa ctattagaca cttatatgat aaaagggaat
    24601 aagaagcgtt tttttacaca aaaaactttg aaaatgtcgt tgacgtacac tacaaaaatg
    24661 aatatactaa tatttgtgtt caacagagaa catgttgcta cgatatttaa gtaattattt
    24721 aagagataac atttaaatta aattcaaaaa gttct
//
LOCUS       DALAGF010000032        24646 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.032, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000032 DALAGF010000000
VERSION     DALAGF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24646
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.032"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(123..1922)
                     /locus_tag="O2692_001199"
     CDS             complement(123..1922)
                     /locus_tag="O2692_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCZ4298834.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQNLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPE
                     PIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDF
                     YAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEKGQETIE
                     SLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(1983..2801)
                     /locus_tag="O2692_001200"
     CDS             complement(1983..2801)
                     /locus_tag="O2692_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCZ4298835.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDINPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(2812..3435)
                     /locus_tag="O2692_001201"
     CDS             complement(2812..3435)
                     /locus_tag="O2692_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4298836.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHKVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            3770..5161
                     /locus_tag="O2692_001202"
     CDS             3770..5161
                     /locus_tag="O2692_001202"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HCZ4298837.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(5311..6057)
                     /gene="recO"
                     /locus_tag="O2692_001203"
     CDS             complement(5311..6057)
                     /gene="recO"
                     /locus_tag="O2692_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCZ4298838.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYAALAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(6085..6984)
                     /gene="era"
                     /locus_tag="O2692_001204"
     CDS             complement(6085..6984)
                     /gene="era"
                     /locus_tag="O2692_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCZ4298839.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(6985..7389)
                     /gene="cdd"
                     /locus_tag="O2692_001205"
     CDS             complement(6985..7389)
                     /gene="cdd"
                     /locus_tag="O2692_001205"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCZ4298840.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(7400..7744)
                     /locus_tag="O2692_001206"
     CDS             complement(7400..7744)
                     /locus_tag="O2692_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCZ4298841.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(7747..8214)
                     /gene="ybeY"
                     /locus_tag="O2692_001207"
     CDS             complement(7747..8214)
                     /gene="ybeY"
                     /locus_tag="O2692_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCZ4298842.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            complement(8215..9162)
                     /locus_tag="O2692_001208"
     CDS             complement(8215..9162)
                     /locus_tag="O2692_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCZ4298843.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
     gene            complement(9466..10164)
                     /locus_tag="O2692_001209"
     CDS             complement(9466..10164)
                     /locus_tag="O2692_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HCZ4298844.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(10181..11170)
                     /gene="floA"
                     /locus_tag="O2692_001210"
     CDS             complement(10181..11170)
                     /gene="floA"
                     /locus_tag="O2692_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HCZ4298845.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(11188..11871)
                     /locus_tag="O2692_001211"
     CDS             complement(11188..11871)
                     /locus_tag="O2692_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCZ4298846.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
     gene            complement(12115..12291)
                     /locus_tag="O2692_001212"
     CDS             complement(12115..12291)
                     /locus_tag="O2692_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCZ4298847.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
     gene            complement(12584..13930)
                     /gene="mtaB"
                     /locus_tag="O2692_001213"
     CDS             complement(12584..13930)
                     /gene="mtaB"
                     /locus_tag="O2692_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HCZ4298848.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            complement(13937..14689)
                     /locus_tag="O2692_001214"
     CDS             complement(13937..14689)
                     /locus_tag="O2692_001214"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCZ4298849.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIEVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            complement(14691..15629)
                     /gene="prmA"
                     /locus_tag="O2692_001215"
     CDS             complement(14691..15629)
                     /gene="prmA"
                     /locus_tag="O2692_001215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCZ4298850.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            complement(15633..16772)
                     /gene="dnaJ"
                     /locus_tag="O2692_001216"
     CDS             complement(15633..16772)
                     /gene="dnaJ"
                     /locus_tag="O2692_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCZ4298851.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            complement(16908..18740)
                     /gene="dnaK"
                     /locus_tag="O2692_001217"
     CDS             complement(16908..18740)
                     /gene="dnaK"
                     /locus_tag="O2692_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCZ4298852.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            complement(18809..19435)
                     /gene="grpE"
                     /locus_tag="O2692_001218"
     CDS             complement(18809..19435)
                     /gene="grpE"
                     /locus_tag="O2692_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCZ4298853.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            complement(19467..20444)
                     /gene="hrcA"
                     /locus_tag="O2692_001219"
     CDS             complement(19467..20444)
                     /gene="hrcA"
                     /locus_tag="O2692_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HCZ4298854.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            complement(20544..21668)
                     /locus_tag="O2692_001220"
     CDS             complement(20544..21668)
                     /locus_tag="O2692_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCZ4298855.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRILKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVKRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNTGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDVIALTNRGKVIGNEVFEAFLIND"
     gene            complement(22231..24054)
                     /gene="lepA"
                     /locus_tag="O2692_001221"
     CDS             complement(22231..24054)
                     /gene="lepA"
                     /locus_tag="O2692_001221"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCZ4298856.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCVLRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            24400..>24646
                     /locus_tag="O2692_001222"
     CDS             24400..>24646
                     /locus_tag="O2692_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCZ4298857.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTAN"
BASE COUNT         7500 a         4675 c         3409 g         9062 t
ORIGIN      
        1 ttacaaattg gaactaatcc caatatttat ttttcaatag tgttgtttaa atgcggtaac
       61 tatcatttac ccttatacat tttgacataa tcattattcg tattattctt taaaatcaca
      121 tgctacatgc gttctttatt cttagcaaca atttgctgta aatagtattt ttgtaattct
      181 acatcgccaa tccttgtagc ttcccttaat ttatgattca atgactcaat tgtttcttgt
      241 cctttttcat taataacatt gacataatca tcaatttcat tttcatatgg ttcgtcattc
      301 aaattatatt gttctaagct aattagtgtt tctctcaact catttgaatt aacatactgc
      361 acagcatcac tgatattata ttgatcattt tccgcataaa aatcatgtaa gacttcgaat
      421 acatatttaa aatgctgatt tgtgaagtta tccttatcaa cactttcata ataatttaaa
      481 aatgtatctt tatctctcat taaatgtttt aaaaatgctc gctccgcttt ttcttgacgg
      541 ctcaaattgt caaattgtgc cataccaatt ggctcaggtt caatgtaacc gccatactca
      601 tcttctggat aataattggc tggtgcttga ttgaattgta tttcgttagc taattgctca
      661 ggactaacat tgaaaaatgg tgcaacatca tttaaagcct tttgttgcaa aatcgatgat
      721 ttcataagcg aaatatcatg acttagttct ttcaaataac gttcatatga aaggtcatta
      781 tgtgcaattt catcttttaa tatactcact ttataatgtg caaatgactt tttgtcattt
      841 tttacaaaag cagtaaatgc atcgttgcca tacttaccaa tgtattcatc cggatccatg
      901 cctgatggca attgtataac aaatacattt agcccttgct gtaacaaatt ttgacctgtt
      961 ttaagtgttg cttcactacc cgcaaaatcc ccatcaaaca ttaatgttat atttgatgtt
     1021 aactttcgta taaaagtaat atgttcatct gacaactgtg tacccattgt tgcaacaacg
     1081 tttttcaagc cagcagtatc agattttata acatccataa aaccttctag taatacgatt
     1141 tcatctaatt ttctaattga tttacgcgct ttatctaagt tgtataacaa ctttcttttt
     1201 tgaaagatag gtgtttcagg actatttaag tattttggtt cttgaccggt atatgttcga
     1261 cctgaatatc caacaattct tccttgcgca tttttcaaag gaaacataat acgatttcga
     1321 aatctatcgt aataactgaa attttcttcg ttacgtgata ataatccggc ttcatatgct
     1381 aattcaatat cgtaaccctt tttttgaaga aaatcatgac aaaaatgtga gctatcgggt
     1441 gcaaagccaa tgcctcgctc tttaataagc gcatctgtaa aaccacgttc ttgtaagtac
     1501 gttaatgctt gttcgccttc gactgtcttt gttaaagcgt aataataaaa ttcttgtatt
     1561 aactcatgca tttcaatcat ttgtaaatca tcagaagcaa tttgaacatt tgagttagat
     1621 tgtgttgcct caatatctac agcaacatta actctatcac ctaattcttt aaccgcttca
     1681 acaaatgata tgtctttaat ttcttgagta aattgaaaaa cattgccacc ttttttacaa
     1741 ccaaaacaat gacaaatttg tttatcttca gaaactgtaa atgaaggtgt cttttcatca
     1801 tgaaaaggac acaaacctat ataattgcgt cctctctttt ctaattttac atattcactt
     1861 accaagtcta aaatgtcggt tttatctttt atttcattaa tgatcgattg atctattcgc
     1921 aaattaaatc acctattaag cattagttat actattatac ctaattttcg acaaaaatga
     1981 aatcatttcg atttattttg ttcaatataa tggattatat cgtttgctgt ttcttcgatt
     2041 gctttttgag aaacatcaat gacaggacat ccaatttcac ttacgatttc ttcaaagtaa
     2101 ttcaattctt cttgaattcg tgcttctgtt gcatatcgag ctgtgtcacc tagtcctaat
     2161 tgttttagtc gctcttttct aatgcgattt aatttttctt cacttatttt aagtgcgata
     2221 catttctttg gattaatatc atataagcca tctggcggtg tcacttctgg tacaatcggt
     2281 acattcataa ctttgtaact cttatgcgct aaatactgag ataatggtgt ctttgaagtt
     2341 ctcgaaatac caagtaaaac aatatcagct ttaggtaatc ctttaggatc tttaccatca
     2401 tcatatttaa ctgcaaactc tatcgcatca attttcttga aatatgcatc atctaatcta
     2461 tgaacgatac ctggctcatt ataaggtttt tcttcaaccg aagcagataa taaatccatt
     2521 aatggcccca tgatatcgac agacttcaat tggaattctg ctactttctc actcatatat
     2581 tgcttcattt caggtttaat aagtgtataa acaatgatag catttgtatc ttttgcaact
     2641 tgaatcactt catcaacatc ttcaaaagat tcaatatatg gatatcttaa taattcattt
     2701 ttacattgct taggattgaa ttgtgaaata cctgccctag caactaactc tgccgtttca
     2761 cctatagaat ctgaagctac gataatttta attttttcca ttacttctca cctattcttt
     2821 atataatgct actaataact tagctattgt tgttttggaa attcttccaa ttacttcata
     2881 cttttgatta tctttttttc ttacaattgg aatcgaatca atttcttttt caatcattct
     2941 atctgctgcg tatatgacta attcgctttc ctctaaataa gtgacattag gcatacgtgt
     3001 catatttaca ctgataggta ctttatgaat atctgctcca atcattgaag ctcttaataa
     3061 atcttttctt gaacacacac caacaaaatc gttatcttca ttaataataa ataatgtact
     3121 tacatcttct aaaaaaattg tacaaatagc gtcataaact gttgtattct ctcttagcac
     3181 aacaggttga gacatatagt ccttaacctc aaattgtcga agtttttcat taaaaaattt
     3241 gccttttgat ttacctgaat aataatatcc aactcgggga cgcgcttcta aaaaacctga
     3301 cattgttaat atcgctaaat ctggtctaag cgttgctctt gttaaattca acttatctgc
     3361 tatttgttca ccagtaatgg gtcctttagt tttaacaatt tcgatgattc gttcttgtct
     3421 ttgactgagt tctataggtc ttcacccctt ttttattcac ctattattat accgatattt
     3481 ctttcatgtc acaaacacat taattttact tgcctttaaa taatctatca attagaataa
     3541 acgtacagcg agttaaggat agtgtaagct taacaataag attggcgcaa cgaatcattt
     3601 taaaataaaa gcgagtgact acactaattt gggtggaacc gcgggttaaa tacgtaactt
     3661 attttgtatt atatacaatt taaaatatta actcgtccca tggaagtaaa gcatatttac
     3721 tatgctgtta ttttccatgg ggcgttttca tgtatgagga gaggtaatta tggcaaaaga
     3781 tatggataca attgtttcat tagcaaaaca cagaggtttt gtgttccctg gtagtgatat
     3841 ttacggtggt ttatcaaaca catgggatta tggtccttta ggtgttgaat taaagaataa
     3901 tgttaaaaaa gcttggtggc aaaaattcat tacacaatca ccgtttaacg ttggtatcga
     3961 tgctgcaatc ttaatgaatc caaaagtatg ggaagcttca ggacacttaa acaacttcaa
     4021 cgacccaatg attgataata aagatagtaa aattcgatat cgcgctgata aattaattga
     4081 agattatatg caagatgtta aaggtgatga aaacttcatt gccgatggtt taagttttga
     4141 acaaatgaaa aaaattattg acgatgaagg tattgtttgt cctgtaagta aaactgctaa
     4201 ctggactgaa attcgccaat tcaatttaat gtttaaaaca ttccaaggtg taactgaaga
     4261 ttctacaaat gaaattttct tacgtcctga aacagcacaa ggtatttttg taaactataa
     4321 aaacgtgcaa cgttcaatgc gtaaaaaatt accatttggt atcggtcaaa ttggtaaatc
     4381 attccgtaat gaaatcactc caggtaactt cattttcaga acaagagaat ttgaacaaat
     4441 ggaacttgaa ttcttctgta aacctggaga agaaatcgaa tggcaaaatt attggaaaac
     4501 ttttgcaagt gactggttaa caagcttaaa tatgagcagt gaaaatatgc gtttacgtga
     4561 tcatgatgaa gatgaattat ctcattactc aaatgcaaca actgatattg aatataaatt
     4621 cccatttggt tggggtgagt tatggggtat cgcaagtcgt acagacttcg acttacgtaa
     4681 acatgctgaa cactctggtg aagatttcag ataccatgat ccagaaacga acgaaaaata
     4741 tattccatat tgtatcgagc catcacttgg tgcagatcgt gtaacattag ctttcttatg
     4801 tgatgcatat gatgaagaag gcgttgaagg tagtaaagat gcacgtacag ttttacactt
     4861 ccatcctgca ttagcaccat ataaagcagc gattttacct ttaagtaaga aattatctgg
     4921 cgaagcgatt aagatttttg agcaattaag ttctaaattc tcaatcgatt tcgatgaatc
     4981 acaatctatc ggtaaaagat accgtcgtca agatgaaatc ggtacacctt attgtgtaac
     5041 attcgacttt gattcattag aagataatca agttacagta cgtgacagag attcaatgga
     5101 acaagttcgt atgccaatct cagagttaga agctttctta actgaaaaaa caaaattcta
     5161 attaaaaaga ctttccacca tatgtggtgg ctatataatt tataaaaaga gatactggtt
     5221 agtaaaattg ccagtatctc ttttctattc aaaaaggtat tcaaataaac acatctatgt
     5281 ggctacttga atacctttta ttattttatg ttattgttcc aatcttttta attggttgat
     5341 tagtttctga cttttaaaaa acatacctgc atattcacga tataacatta aaatgatatc
     5401 tgacatttca tcaataattt cttgatggat attcaatgaa ttcattttat ctatcggtaa
     5461 tttttgtaat acatctaata aatatagtgt tttattcgat aatataactg catgtacatc
     5521 tttagaagcc tcttgccttg aaatcgcacc gtcaaactta aaactataac ctattaaatc
     5581 tgcttgtgtg tcattaccac tcacagcaca gcgattaaat gatgcagtaa aaccaaatcg
     5641 tttcatgcac tttaacataa ctacgactga cattaactgt gcagatgtac ctgattctat
     5701 tttttcaaga acaaattgta ataattgata gttatatggt gcaatgtcac cttcatccat
     5761 tgagcgctca atagtttcag ctgccagagc ggcataactg cttacgtaaa ggtccatttg
     5821 taatttataa tgttgactaa taacatctac agaatttaac gttcccatac ctcgccactg
     5881 attgtaaata aacaaaccat aaacaaacaa ttgcgtttgc gcttgtaaac ccgtcttaac
     5941 ttttttagca cgccttgcca taagtggtac ttttgcacca tgctcattta aaatcgtgat
     6001 aattttatca gattcaccat aatcaactgc tttgatgata atcccttttt ggcgcattaa
     6061 caattatctt caccactttt aagattaatc ttggtcttca acataaccaa tttggcgaat
     6121 aaagttaact ttgtttcgcc agtctctttg aactttgacc cataattcta agtaaacttt
     6181 agagcctaga agcatttcta tatcacgtct cgcacgtttt cctacttctt ttaacttttt
     6241 accgcctttt ccaatgacaa ttcctttttg cgaatctctt tcaacatata tagttgcttc
     6301 gatatgaaca cgatcttcgc tttctttaac catacggtcc acattaacac caatcgcatg
     6361 agggatttct tcacttgtaa gatgaaggat tttttcacga atgatttcac ccactacaaa
     6421 ttgttcagga tggtctgaaa tttgatcatc tggataatat ttaggtcctt cgggtaaata
     6481 cgtctttaaa acatcaataa aatgatcgac atttagccct tctaatgctg aaataggtac
     6541 aatctctgta aagtccatat aactttgata ttcttcaatc tttggcatta attcatctgg
     6601 atgcactaaa tctattttat ttaatactaa aaatactggt gtcttaacat ttttcaacat
     6661 ttctataata tattcatcgc ctcgtccaat ttcctcattg gcattaacca taaacatgat
     6721 tgcatctatc tcagataatg tatttttagc gactttcatc atatagtcac ctaatttgtg
     6781 tttaggttta tgaatacctg gcgtatcaat gaatataatt tgcgcgtcat ctcttgtcat
     6841 aacaccttga attttatttc tagttgtttg agctttatcg gacatgattg ctattttatg
     6901 gccgatcact ctattaacaa atgttgactt tcctacattt ggtctaccta taattgaaac
     6961 aaatcctgat ttatgttctg tcatttattc taaatccttt cctgaaaatc caaatggtag
     7021 taactctgcg actgtcatca taaccatatc tcctttatga tttgtcatat acacaggcat
     7081 atcatcatca cataattcct tcaaaacttg acgacatgca ccacaaggtg atgacggttt
     7141 atctgcatct acggttacag ttattgattc aaaatcacct ggtctgtatc cttgagaaat
     7201 tgccgatacc aaactagctc gttcagcaca tatcgataat ggataagaag cattttctac
     7261 attggtacca taaaaagttc taccgtcttt cgtttttaaa taagccccta ctttaaattg
     7321 actgtatggc gaatatgatt cttgttgtgc ttttctaact tcttgaaaat aatgaggttg
     7381 ataactcata tatgcctccc taaaataacg ctataaaatg tggtaaaaat actattaaac
     7441 ctataataaa tgctaatatt gaaactataa gtacactaaa agccgcaata tctttagcgt
     7501 atttagctaa atcatgatat tcaacggtca ctaaatcgac aacatattca atagcagtgt
     7561 ttaaagcttc aacagtgaga actaatgcaa tagcaatgag tataaatatc cactcaatcc
     7621 gattaatatt tagtacgaga ccaaagacaa tagcaacaat cattgcaaac acatgtaaaa
     7681 gaaatttata gtctttttga attaagattt tcagcccatc aagtgcatat ttaaaccttt
     7741 tcataattag tctcgtgtta atccatatgc gtttaatatt gtatcttgtc gaccaaacat
     7801 ttccttttca tccgcttcag tcatatgatc ataacctaat agatgcaaaa atccatgtaa
     7861 tgctaaaaat cctaattctc gttcaaaaga atgtccgtaa ttgtttgctt gttcttgcgc
     7921 tacatccgta cagataatta tatcccctaa aacacgtggt atatcaagac cactaaaatc
     7981 aatctctggc tcatcttctt ctaaagcaaa tgagattaca tctgtaactt tatctttatc
     8041 tctatatgtt cgattaattt cttgtatttc ttgtttatct acaaatgtaa cagaaagctc
     8101 agcatcgtct tctatatgct cttctttttt agcaaattct aataaatctt caatttgttt
     8161 ataccaagca tctttaacta agcctgtgtg atcgctaaaa tctatcgtaa acatttaatt
     8221 ctctccttca taatgttcaa tgatcttact taccaatgga tgtctgacta catcgctctg
     8281 atctaatttc aatatactta tacctttaac gttgtgtaac ctactgaccg cttccttaag
     8341 tccactttta acacctttag gtaaatcgat ttgagtttgg tcaccagtaa ctaccatttt
     8401 tgagccaaaa cctagtcttg ttaaaaacat tttcatttgc gcatgtgtcg tattctgcgc
     8461 ctcatcaaga attacaaatg catcttctaa tgttcgtccg cgcatatatg caagtggcgc
     8521 tatttcgata atgcctcttt caataaatcg ctctgtttgt tcacgcccaa gaacagtata
     8581 tagaccatca tataaaggtc ttaaatatgg atctaccttt tctttcaaat ctcctggtaa
     8641 aaatccaagt gactctcctg cttcaacagc aggtcttgtt aatacaatac gtttaacagc
     8701 acctttacgg agttgctttg ctgcataaac tacagctaag aatgtcttac ctgtaccagc
     8761 aggacctata ccaaatacta aatcattatt tttcatggca ttaacatata tacgttgccc
     8821 catcgttttc gcacgaatcg tcttaccaaa tgcatcttta gttatctctt catcatataa
     8881 atctaacaga tgttgaattg tgttattatg cgccatttta atagctgctt caacatcttt
     8941 aattgtaata ttattaccta aatcaataac cttcagcaaa ttgattaata ctgattccgc
     9001 tttttctacg ttttctattt ttgtaccttt aacggcaact tcttgtcctc ttgcatggat
     9061 gacaacatcg aaactctctt caattgcttt taaatgttca tcattatttc caattaaagc
     9121 ttgagattgg ttcatatcgt ctatttgtat aattccaggc atacacgcgc tccttttcat
     9181 atatcattta tattcattct atgatgcttt aatatctact taattatatc atgcaattga
     9241 ttgatttaaa catctttgca ttgacttact catttttctt taatttaaaa taatcattta
     9301 tacttttcac cttgtcataa aagcagattc aattttagac taatcaattt tttgctgtaa
     9361 tttaaataac taatatttct cgaaaaattc tctttgtttt ttaatttttc ttaacgtaaa
     9421 aaaacaagca actaccatta cgatagtgct tgttttcaaa aaattttata attgttttgg
     9481 tttagctaaa atttctgacc atatcatacc attgattact tcatctttat caaattggaa
     9541 cgctgattta gtcaaatctt tttcgacatt ttgagaatta agcagctgct ttaatttcaa
     9601 acgttttgta cgttcagata agtatttatc ctcaataata tctctagcac gtttttccat
     9661 tttagcaatt tgcttttctt tttctctatc aatgtcagta cgaatcgttt taatatcatc
     9721 tctaagtgat ttttctaatt gacgtctaat ttcgtcagaa ttatcttgtc tagaaggtct
     9781 atgtttctgc tccaccggtt ctgctttagg cactgtgatt ggtttctctg tcatcggttt
     9841 tgtttcttgt tgttttttag gtgccatagg ttctacaaca ggtttcgatt taggctcttc
     9901 ctttggaagt tcatcgaata aaggtggtaa cgtatcatca tattttcgtt tcgatgattt
     9961 cttttcttct tcatttaatt cttcacttat ttctttaaac gttcgctcaa tttcttcaaa
    10021 aaagccacct ttttttggtt cattatcggt agatgttttt tgaggtggct tttgattttg
    10081 tctatcttta tgactatttt cgcgcatagt agtaatgata gaaatgatca ctgatatgac
    10141 aaaaattaga ataccgacac tcatttaatc acctctcgac ttaatgttca ggtgactcat
    10201 catcactttg atcagttcgt ttattaattg catttctcat gcctgtatca gcttcgatat
    10261 ttttcaaatt ataataatct ttaacactga tattacctga acgtaatgct tcagccatag
    10321 ctaatggtac ttcagattcg gcttcaacta ctttagcatg catttcttgt acacgcgctt
    10381 tcatttcttg ctcagttgct acagccatag ctctacgttc ttcagctttt gcttgtgcaa
    10441 tatttttgtc tgctaatgct tgttcagttt gtaagtctgc accaatattt ttactaatat
    10501 caacgtcagc aatatcaatt gataaaattt caaatgcagt acctgaatct aaacctttgc
    10561 ttaaaactgt tttagaaata ttatctgggt tttcaagtac ttctgtatga tgcttactag
    10621 aaccaattgt tgaaacgata ccttcaccaa cacgtgcgat gattgtttct tcaccagcac
    10681 caccaacaag tcgagcaata ttagctctaa ctgtgatacg agctttggct ttcacttcaa
    10741 taccgttcat tgctacacct gcgataaatg gtgtttcaat gactttagga ttaacagaca
    10801 tttgaaccgc ttctaatacg tcacgtcctg caaggtcaat tgcagcagca cgttcgaaag
    10861 gaagatcaat gtcagcacgt tgtgcagcaa tattagcgtc aacaactctg tcaacatttc
    10921 ctcctgctag ataatgcgat tctaattggt ttgttgttaa tgctagtcct gctttgtgcg
    10981 ctttaattaa tggcgctata acttttcttg gagatacacg acgtaaacgc ataccaacca
    11041 atgtacctat accaacatga acgccagctg ctaacgctga aatccataaa ccaatgggta
    11101 caaatgagaa taaaataagt aatgcaacta ctataataac tgctattacg ataaaactta
    11161 aactaaacat ggtatcgctc cttttaatta atctacttcc ctcacaacta ctcttgttcc
    11221 ttcaacttca aggattttta ccgttttatt gcgcaaaata aagttgccat ctgaaacagc
    11281 atcaatacgt tcattttcac aaaaaataat ccctgcaggt cgaagatctg taactgtttg
    11341 agcagtcttt cctacgaggt gcgagcggtt atcatgagaa ttgtaacctg actcagaatt
    11401 agttgaatct tttaagataa ctttatccaa aaacggaatc tttctgttga aaatcttcac
    11461 taatatcacc cattctacaa tcgttaaaat caaggcaacg ataacattcg caagcataaa
    11521 tagcaaatta tcaccgagcg ttgttatgct tatagttatc agtatcatgc caataatacc
    11581 aattactgca ccaactacaa ataattcaat tacaactaat ataacgccaa ttgagaatat
    11641 taagatagaa tgcatattga catttccttg gattagaaat cccaaaaata aaataagtaa
    11701 tgataatgtg gcgataatac cagctgcatt gattttttta gagtaaagtt gatatacaaa
    11761 tcctaagaat gtcaaacagg ttaatattaa cgtaaaaata ggttgaacaa ttatattgct
    11821 aacttgttca acccaagtat ctccagccgt tgattccaag atagttgtca tttgtaaaaa
    11881 attattataa ctcacaattt cacctcgccc tcacattaat tatacatgaa attgaatata
    11941 ctttatagtt ataagaacct atagaatatc aatttgatta tttataaact atataacttt
    12001 ttttaattaa aaattgacaa caaaaaaaca tcacatatta gtgtcagaca ctaacttgtg
    12061 atgtttgttt tatataatta atattcgtgt tgagggagtc aacagaggta ttaattattt
    12121 gaatttacgt ttacgtgcag cttctgattt ctttttacgt tttacgcttg gtttttcgta
    12181 aaattcacgt ttacgtactt cttggattgt tccactttta gaaactgaac gtttaaatct
    12241 acgtaacgca tcttcaagtg attcattttt acgtactact gttttagaca tctgtatttc
    12301 cctccctcca aatatcaacg gaactttata gtcaattgca cagtaaaact atgcatttat
    12361 aagtataata tatctgtaat ttatggtcaa ttagtaaatt gttttttatt tgaaacatat
    12421 ttcacatcaa aatcacaaag acttttagat tttgttctaa aaatctctta ataatttatt
    12481 taatgagaag agttgcttat atagtaaatt gtgaagccgt taaaacaacg ttacaaaacc
    12541 tatatcttta atacggaacc atatggtatg aatcaaggaa tacttaaact aaaacttctc
    12601 tatcagattt atttgttgcg aaatcaacaa ctttaattgc ttgcccttca tttaatggat
    12661 aatttgcttg cgtaatttta acttttacaa tttgacctat gagtgattcg tcaccttcaa
    12721 attgtacttt catataatta tctgcatatc caactaatgt accttctgtg tcaccctgtt
    12781 cctcaggaat tacttcaagc acatcttgat caaatttaga cgcatataac tttccgagtt
    12841 gattgcttag cgtaattaac ttatgcaccc gttcattttt aatttcttca tcaatttggt
    12901 catccattct tgcagctggc gtgccaattc taggagaata agggaaaaca tgcagttcag
    12961 agaacttatg ctttacgata aaatcatatg tttcttggaa ctcagcttca gtttcacctg
    13021 ggaaaccaac aattacatca ctcgtaactg ccaagtctgg taaagcttta tgcaattttg
    13081 ttaatcgttc tgaaaatcta tccattgtat acttacgtct catacgtttt aatactgtat
    13141 ctgaaccaga ttgtaatgga atatgcaaat gacgcacaac ttttgttgaa cgttctaaaa
    13201 cgtcaattac ttcatctgta agttgacttg cttcaattga agaaattcga attcgttcta
    13261 atccattaat cgtttcaaga tcacgtaata attgggccaa gttataatct tttaaatctt
    13321 gaccatatcc acctgtatga attcccgtca atacaatttc cttatatcct gaattcacta
    13381 gttgcgtcgc ttgttcaact actttttccg gatctcttga acgcattaag ccacgagccc
    13441 atggaataat acagaatgtg cagaagttgt tacaaccttc ttgaattttt aatgacgcac
    13501 gtgttctatc tgtaaaataa gggacatcta attcttcata tttacgattt ttcatgatat
    13561 ttccaacacc attaattggt tggcgttctt tacggaattc gtcaatgtaa cctaatagtt
    13621 tatgtctatc ttgtgtacca actactacat cgacaccagg aatttccata atttcagctg
    13681 atgaagtttg cgcataacaa cctgttacac agattacagc atcaggattt tgtcttattg
    13741 cacgtctaat tatttgacga ctttttttat cacccgtatt cgttactgta caagtattaa
    13801 taacaaatac atcagcattc gcttcaaagt caacgcgctc atagtttgct tctttaaata
    13861 attgccagat tgcttcagtt tcataatggt ttactttaca acctaatgtg tgaaacgcaa
    13921 ctgttgacat aaatattcac cccattaatt ctttttcata acttattgca cttaacgcat
    13981 acaatggcgc agtttctgcc cgtaaaattc tcggcccaag accaacaact gtactagtat
    14041 tactaaataa tgaaatttca ttttctgaca aaccaccctc aggaccaaaa atcatcaaca
    14101 ctttatcctg agcattgaat tgttgtaaag tttgcttgaa attgcttaac tcaccatctt
    14161 ttgcttcctc ttcatatgca ataagaatat agtcataatt atcaatagta tcacaaatta
    14221 attttaaatt cgactcgaat tgaatagatg gaatcactaa acgatagctt tgttcagcag
    14281 cttctttaat tattttttgc caacgctcta tctttttggc aacttttgcc tcgtttaatt
    14341 taacaattga acgttccatg ctcacagcta taaatgatga agcacccaat tcagtagctt
    14401 tttgtagcaa ccactcatat ttgtcagctt tgattagtcc actgcaaatc gtaacatcaa
    14461 ctggcaattc tgtattaata ttttgttttt cttttaaatc aacttcaatt ttatcacttg
    14521 ttatgtcagc aatttcacat aaataaactg tttgatcatt aaaagttaaa ataattttac
    14581 taccaacatc atatctcatt acatttgtta tatgatgaat atcttctttt tttgtaataa
    14641 aaaaacgctg acttacatca gcgttttggt ctatgaaata acgttgcaca ttattcactc
    14701 actttctggc caacaagaca aacccaaccg ttgtcatgtt gttctgaaat aattttaaaa
    14761 cctacacgct ccatatgtga ctgtatacct tcatacttct cttttataat accagaagta
    14821 ataaaataac cgccttcatt tagagtatta taagcatctt caatcatttc atcaataata
    14881 tgcgctaaaa tatttgctat tacaatatca aatttttctg tttcgtcttt caataagtta
    14941 cctggaacag cttcaattaa cgtttcacaa tgatttcttc tgaagttttc tttagctaca
    15001 ctcactgcca tttcatcaat atccaacgct ttaatacgtt ttacaccgat tagatgactt
    15061 gcaatactta atatacctga gccagtacca acatcaatta ctgaatgctg tggcaataca
    15121 tatgtttcta ttgccttcaa acacatactt gtagtcggat gatcacctgt tccaaaagcc
    15181 atacctgggt cgagctcaat gcaaagctct tcatccgctt ctttagcata tgtttcccaa
    15241 ctaggaacta ttgtgaactt cttcgacgct cggaatggat ggaaatagtt tttccattca
    15301 ttttcccaat ccgtctctgc aataatttgc tcactgaatt gaacgttatg ttgatcaagt
    15361 tcatctaaat ttaataactc atctttaatt tgctgtcgca acttatcatc ataagtcatt
    15421 tcattaaaat aggctttcaa tcttactccc ttatctggat aatcctcttt tttcaaagcg
    15481 taaatttcac cgtatttatc ttctggttgg ttaattaaat catctgaatc ttctatcacg
    15541 acaccatttg atccatgatt ttcaagtata ttggtagcca attctactgc ttcatgatta
    15601 ataataattg aaagctctgt ccagttcata ctttattctc ccttaaagaa tctttttgct
    15661 ctatctttaa aattcgaagg ttgttcatta atttcttcac catttaattg ggcaaattct
    15721 ttcattagtt ctttttgtct atctgttaat ttagtaggcg ttactacttt aatatcaaca
    15781 tataaatctc cgtatccata gccatgaaca ttttttatac ccttttcttt taagcggaat
    15841 tgcttacctg tttgtgtacc agcagggatt gttaacataa cttcattatt taatgttggt
    15901 atttttattt catcgcctaa agctgcttgt gggaagctaa catttaattt gtaataaata
    15961 tcatcaccat cacgtttaaa tgtttcagat ggtttaactc taaatactac gtataaatca
    16021 ccagcaggtc ctccattcac gcctggagag ccttcaccag ctaatctaat ttgttgttca
    16081 ttgtcgacac cttcaggtac tttcacttct aatttaactg ttttattttc agtacctttt
    16141 ccgtgacatg ttggacaagc ttcttcaaat tcttgaccac ttccattaca tttaggacaa
    16201 acttgttcag tacgaactct acctaaaatt gtgttttgtt ctacagctac atgaccagcg
    16261 ccattacagt aactacaagt ctttttactt gttccaggct ttgcaccatc accatgacat
    16321 gtttcgcatg ttacatcttt acggattgaa atttcttttg ttgtaccaaa taccgcttct
    16381 tcaaatgtta atgtcattgt atactgaaga tcatcacctt tttgcggtgc atttggatct
    16441 ctttgtctgc cgccaccgaa gaaagagcta aagatatctt caaaaccgcc gccaccgaag
    16501 ccactaaaac cgccaaagtc agagccattg aatccttgtc caccaaaacc ttgtggacca
    16561 tcatgtccaa attgatcata gcttgcgcgt ttattatcat cacttaaaac ttcataggct
    16621 tcagaaattt ctttaaactt ttcatctgca ccttcttctt tgttaatatc tggatgatat
    16681 tttttcgaaa gctttcgata cgcttttttg atttcatctt ttgaagcatc cttactaatg
    16741 cctaaaactt cataataatc tcttttggcc acagctatct ctccttttct taattaactc
    16801 atatagttta acgtaatatg tcatactatc caaataaaaa gccaaagcca atgttctatt
    16861 gactttgact tttcagatca tgacaacatt ctaattgtat tgtttaatta ttttttgtcg
    16921 tcgtctttta cttctttaaa ttcagcatct tctacagtac tatcattgtt ttgaccagca
    16981 ttagcacctt gtgcttgttg ttgctgttga gccgcttgct catatacttt tgctgataat
    17041 tcttgaatca ctttttcaag ttcttctttt ttagatttaa tatcttctat atcttgacct
    17101 tctaaagcag ttttaagagc gtcttttttc tcttcagcag attttttatc ttcttcaccg
    17161 atattttcgc ctaaatcagt taaagttttt tcaacttgga atactagact gtcagcttcg
    17221 tttcttaagt ctacttcttc acgacgtttt ttatctgctt cagcgttaac ttcagcatct
    17281 tttaccatac ggtcgatttc ttcgtctgat aatgaagaac ttgattgaat tgtaattctt
    17341 tgttctttat ttgtacctaa gtcttttgca gttacattta caataccgtt tttatcgata
    17401 tcaaacgtta cttcaatttg aggtttacca cgttcagctg gtggaatatc agtcaattgg
    17461 aatctaccaa gtgttttatt atccgcagcc attggacgtt caccttgtaa tacgtgtaca
    17521 tctactgatg gttgattatc tactgctgtt gaatagattt gagatttaga tgtaggaatc
    17581 gtagtgttac gttcaattaa cgtattcata cgtccaccta aaatttcaat acctaaagat
    17641 agtggtgtta cgtctaataa tactacgtct ttaacgtcac ctgtgataac gccaccttgg
    17701 attgcagctc ccattgccac tacttcgtcc gggtttactc ctttgttagg ctctttaccg
    17761 atttcttttt tgacagcttc ttgtactgct ggaatacgag ttgatccacc aactaagata
    17821 acttcatcga tatctgagtt tgttaagcca gcgtctttca ttgcttggcg tgtaggttcc
    17881 attgttcttc taattaatga atctgataat tcttcaaatt tagaacgagt taagtttact
    17941 tctaagtgta atggaccgtt ttcaccagct gagataaatg gtaatgagat ttgagtttgt
    18001 gatacacctg ataagtcttt tttagctttt tcagcagcat ctttcaaacg ttgtaatgcc
    18061 attttatctt gagataagtc tacgccattt tcttttttga attctgcaac taggtagtca
    18121 ataattactt ggtcaaaatc atcaccgcca agtttgttgt caccggctgt tgatagtact
    18181 tcgaatacac cgtcacctaa ttctaggata gatacgtcaa atgtaccgcc acctaagtca
    18241 aaaacaagaa ctttttcatc tttatcagtt ttgtctaaac catatgctaa tgctgcagct
    18301 gttggttcat taatgatacg ctcaacttct aaaccagcaa ttttaccagc atctttagtt
    18361 gcttgacgtt cagcatcgtt aaagtatgca ggtactgtaa ttacagcttt gtcaactttc
    18421 tcacctaaat agctttcagc tgtatttttt aagttttgta aaatcatagc tgagatttct
    18481 tgtggtgtgt atgatttacc ttcaatatct actttataat cagtacccat atgacgttta
    18541 atagattgaa cagtgtttgg gtttgtaata gcttgacgtt ttgctacttc accaacttga
    18601 gtttctccat ttttgaaagc tacaacagat ggtgttgtac gtgaaccttc agggttttga
    18661 attacttttg gctcatcgcc ttctaatact gttacacatg aatttgttgt acctaagtct
    18721 ataccaataa ttttactcat aataaaattc ctccatttaa tcattaaatt aatttaattt
    18781 taaacaatgt cttttcgcca aatttaagtt attggtttac tttgaccatt gatggtctta
    18841 atactctatc tttaagcttg tatccttttt gtagttcttg agtgatttcg ccagattcaa
    18901 aatcagggtt atcatcttga actacagctt ggtgaatatt tggatcaaat gcttcacctt
    18961 cagttttaat aacttcaaga ccattatctt ttagtgcgtt aatcaaactt tcatgcacca
    19021 tttgtacacc tttttgaaga gatttaaaag tctcatcatc accttcaatt tgaagtgcac
    19081 gttctatatt gtctattgct ggtaaaatat ctgttaacac acgttgtgct tgatatgttt
    19141 tgtttatttc attttctttt tgaattctac gcttataatt ttcaaactca gcgtagagcc
    19201 ttaaatattt ctcttcgttt tcatctgcta attgttgaag ttcattaatt ttttgatctt
    19261 ttggatctat ttcttcaata acattctcgt cagacgtttc ttctattgct tcatcttgta
    19321 aatgaccttt actttcttca gcttgttcaa ctgaatcatc aatattttgt ttgacgtttg
    19381 tttcttcaac tgttgattca gtgttttttt caactgattc gtctttattt gtcattttct
    19441 gtcctccaat actttctaat ccatcattac caaattctat ttaataattg aatgacattt
    19501 tgataatgca tagctgtagg tccaatcaca gcgatttgac cttttaacgt ttcatcaaaa
    19561 tgatattgac ttgttacaat tgaaatatca cttaagctgt catcaatttc attaccaatt
    19621 tttacattaa tatttggtga agatatatct tgtaataatt ctgcaattct atttgattct
    19681 atatattgta gaatgggctg aattgaagat acattacttt cattcaatgc atcaataagt
    19741 ttaacctttc cacccatata aatgctatta ctttgattag aaatatgatt attcatcgta
    19801 tttaacaatt tattgataaa aatttcttcc tgctctgatt gaacaaaaga gacaatatca
    19861 tcttgtaaat tctgattaaa ctcagttagt ttgtttgtaa caaaatttga tattgtattt
    19921 agtttgtcat tattaaacgg tatgtctgaa gcaagatgta catgctcaac atgacctgat
    19981 gaaaatacga taaccattat aactaaatta ggattagcac gaatcaagtg tacattattg
    20041 ataatatctt gtttatgatt aggatgaaca actaaagttg tatattgaga tatatttgat
    20101 aattcatctg caaaatatgt caatgctgat gatacatcat attgattctc aactaacaat
    20161 tgatttaatc gtcttaattt atttgttttt tgatgagatg tttgttcaag taaacgattg
    20221 acataatacc taaaacctaa ttgtgatggc gaacgccctg aagaactatg tgtcttctcg
    20281 atatagttta aatcttcaag ctgtttcatc tcatttctaa ttgtagcagg actaacattc
    20341 aagttatgtc gctcaattag tgttttagaa ccaacgggtt gtccaaaatc aacataatcc
    20401 tcaacaattg cgtttaatat actcaattgc ctatctgtaa tcatgttttc acctcattag
    20461 cactcactta tctcaagtgc taattataat ttatcaaatt ggtcaaagta agtcaatgtt
    20521 aaagactcga aatttcaatt tttttaatca tttattagga aagcttcaaa aacctcatta
    20581 cctatgactt tccctctatt tgtaagtgca atcacatcgt tcttttctac aattaattcc
    20641 ttctctttta aattatttat tgtttgacca aagacacttt caatagattg gtcaaacttc
    20701 tttttgaacc tactactact cacaccttca tttaaacgca acccaagaaa catttcttct
    20761 tccattctct cagtcaaaga aggtttattt gatactaaaa ttgctttact ttctttattt
    20821 atagctttga tataatgatt cactggattg atattcgtat aacgcacacc atctacataa
    20881 ccacttgcac ctgctccaaa tccataatat tcctcattaa accagtaaac cttattatgt
    20941 tctgattcat ggccatctaa tgcaaaatta gatatttcgt attgatggaa aggagattgt
    21001 tctatcttag acatcagcaa ctgatacatg tcagcaccta aatcctcatt aggaagtttg
    21061 agcaagcctt ttctatacat attataaaat tgggttttag gttcaagtat taagccgtaa
    21121 ctcgaaatat gttgaatatc catatctaaa gctagatcta aactttgttc aaaatcttca
    21181 atcgtctgtt tcggtaaatg atacattaaa tctaaactga ttgatttaat acctgtgttt
    21241 ttagcattta acaccgaagt gtaaatatct tcagtattgt gcgttctacc taaaacagac
    21301 aataactccg gcttgaatgt ttgaacgccc attgaaatcc tttttactcc atatttctct
    21361 aatagttgga ctttctcttt agttaactca tcaggatttg cttcaaatgt atactcgcct
    21421 gtgattgtaa acgtatcacg tattgcttta agtaatcttt ccaactgatt aatagaaagg
    21481 gccgttggtg tgccgccacc tacatacatg gtctttaaga tcctatattt tgctgtagac
    21541 atttctgtga ttagtgcatc taagtactca tctacaggtt gattctgtat aaaatattta
    21601 ttgaaatcac aatatgtaca tattcttaca caaaatggaa tatgtatata tgcactttgt
    21661 accgtcattt taatgccccg cttctcagac tttttatcca atcggctata tcctgttgga
    21721 ctatctgcaa ttattcttac ttttaacttt aacgtttaat caatgattgt ctaatatatc
    21781 gattcatttt aatatttatt cttgtttaga gggagtgaga tagaaataat aaagaaccac
    21841 taatgattta ttatgtagtg gctcttacac attagccaga tgtaatatgt ccttaaaaat
    21901 aaaattacat gagtaaaact catgcataag ggagtgggac agaaatgata ttttcgcaaa
    21961 atttatttcg tcgtcccacc ccaacttgca ttgcctgtag aatttctttt cgaaattctc
    22021 tatgttgggg ccccacccca acttgcacat tatagtaagc tgacttttcg tcagcttctg
    22081 tgttggggcc ccattgttat aagctcattt taactctgtc gatggctgcc aatataacat
    22141 tgtaaagccc aggacgttaa tttatcctag gctccctctt tttagttatt aaactttatt
    22201 cattgtaaat tgttaattga ttttttaaaa ttattcatca tccattttca atacagccaa
    22261 gaaagcatct tgtggaattt caacattacc aactgctttc atcttagctt tacctgcttt
    22321 ttgtttttca agtaatttac gtttacggct tatgtcaccg ccataacatt tagctaaaac
    22381 gtttttaccc attgatttaa tatttgtacg cgctacaatt ttttgtccta ttgcagcctg
    22441 tacaggtact tcaaattgct gtcttggaat taacgtttta agtttttcaa ctaatgcttt
    22501 accacgttca tatgcaaaat ctctatgaac tatgaagctt agcgcatcca ctttatcacc
    22561 atttaataaa atatccatct tgactaaatt actttcttta ttttcgatga attcataatc
    22621 aaatgatgca tatcctttag tattagattt aagttgatcg aagaaatcaa atacaacttc
    22681 agctaaaggt aattcataaa caatatttac acgaatatca tctaaatagt ccatatttat
    22741 aaattgtcca cgtttacgtt gacataattc cattactgca ccgacatagt catttggaac
    22801 catcatagtt gcacgaacat atggctcaaa tattttatca attttatcac gatctggcat
    22861 ttgtgctggg ttatcaaccg tcacttctga accgtccctt aaaacacatt gataaattac
    22921 agatggtgca gttgcaatta attcaatacc aaattctctt tcaattcttt cttgaattat
    22981 ttccatgtgt aacataccta agaaaccagt tctataacca aaacctaatg cttgtgacga
    23041 ttcaggctca aattctaatg atgcatcatt caattgtaat ttttctaatg cttctcttaa
    23101 atcattataa tttttgttat ctattgggaa cagtccgcaa tataccattg gattcatttt
    23161 cttataacct tgcaatggtt ctgatgcagg tctactagct aatgtgatgg tgtcaccaac
    23221 cctagaatca tcaacatttt taatacttgc aataatataa ccaacatcac caactgttaa
    23281 ttcatcaact ggaagctgct taggtgtatt aattccaact tctgttactt cgaactcttt
    23341 accagtggcc atcattcgaa ttttatctcc ggctttaaca acaccgtcca caattcttat
    23401 cgatgaaatt acccctctat atggatcata ctcagaatca aatattaacg cttttagtgg
    23461 tgcttctggg tcaccatctg gagctggcac aacttcaact attttctcta gtatctcttc
    23521 aattccaatg ttagatttag cacttgctaa aacaacatcg tcttggtcta aacctatcat
    23581 atcttcaatt tcttgtttca cgcgttcagg ttctgcagca ggtaaatcaa ttttgttaat
    23641 aacaggcaat aactctaact cattatctaa tgctaaataa acatttgcta atgtttgtgc
    23701 ttcgatacct tgagccgcat ctactactaa aatcgcgccc tcacaagctg ccaaagaacg
    23761 tgacacttca tatgtaaaat cgacgtgtcc aggcgtatcg attaaatgga atgtataagt
    23821 atttccatct ttagcttcgt actttaaacg tactgcgttt aatttgattg taataccacg
    23881 ttctctttct aaatccattg aatctagtaa ctgatcttgc atatctcttg tttcaactga
    23941 tttggtattt tctaaaattc tatcagccaa tgtagatttt ccgtggtcaa tatgtgctat
    24001 aatcgagaaa ttccttatat tctctcttct ttttaagcgt tgctcattat ccattttatc
    24061 cttctcactt tcataaaata aacccgtatt atctttcgca tctttgatat aataacgttt
    24121 ttaataggta aatgcaaacg ccataatctt ggatgaactt tgaaattata acaatcttta
    24181 tttaaataca tacttgcgat atgttaatat gttaattacg tacttataat tataaaacgt
    24241 attgattgtg tttccaacac aatttattaa atctgcaaaa ttttaagcta accccatcaa
    24301 ataaatgatt gcacaacggt tagacttttg ttaaaatatt tcttgttgta atcaaataaa
    24361 attttgataa gatgaactca cttttaggag gtgacagaaa tggcaaatat caaatctgca
    24421 attaaacgtg taaaaacaac tgaaaaagct gaagcacgca acatttcaca aaagagtgca
    24481 atgcgtacag cagttaaaaa cgctaaaaca gctgtttcaa ataacgctga taataaaaat
    24541 gaattagtaa gcttagcagt taagttagta gacaaagctg ctcaaagtaa tttaatacat
    24601 tcaaacaaag ctgaccgtat taaatcacaa ttaatgactg caaata
//
LOCUS       DALAGF010000033        24281 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.033, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000033 DALAGF010000000
VERSION     DALAGF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24281
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.033"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            3..146
                     /locus_tag="O2692_001223"
     CDS             3..146
                     /locus_tag="O2692_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298858.1"
                     /translation="MLTAIYFSIFTFYISQYSLKLKTLYNIETDYNYKIVLLLKEGKT
                     HES"
     gene            136..660
                     /locus_tag="O2692_001224"
     CDS             136..660
                     /locus_tag="O2692_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCZ4298859.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFCKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRNLRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      647..744
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            819..1910
                     /gene="gcvT"
                     /locus_tag="O2692_001225"
     CDS             819..1910
                     /gene="gcvT"
                     /locus_tag="O2692_001225"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCZ4298860.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            1930..3276
                     /gene="gcvPA"
                     /locus_tag="O2692_001226"
     CDS             1930..3276
                     /gene="gcvPA"
                     /locus_tag="O2692_001226"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HCZ4298861.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            3269..4741
                     /gene="gcvPB"
                     /locus_tag="O2692_001227"
     CDS             3269..4741
                     /gene="gcvPB"
                     /locus_tag="O2692_001227"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HCZ4298862.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSKHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(5106..5492)
                     /locus_tag="O2692_001228"
     CDS             complement(5106..5492)
                     /locus_tag="O2692_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCZ4298863.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            5650..6480
                     /locus_tag="O2692_001229"
     CDS             5650..6480
                     /locus_tag="O2692_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCZ4298864.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIEFKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            complement(6544..6762)
                     /locus_tag="O2692_001230"
     CDS             complement(6544..6762)
                     /locus_tag="O2692_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298865.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLISFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(6776..7357)
                     /locus_tag="O2692_001231"
     CDS             complement(6776..7357)
                     /locus_tag="O2692_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298866.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            7462..8523
                     /locus_tag="O2692_001232"
     CDS             7462..8523
                     /locus_tag="O2692_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCZ4298867.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            8549..9106
                     /gene="efp"
                     /locus_tag="O2692_001233"
     CDS             8549..9106
                     /gene="efp"
                     /locus_tag="O2692_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCZ4298868.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
     gene            9580..10044
                     /gene="accB"
                     /locus_tag="O2692_001234"
     CDS             9580..10044
                     /gene="accB"
                     /locus_tag="O2692_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCZ4298869.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQAQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            10044..11399
                     /gene="accC"
                     /locus_tag="O2692_001235"
     CDS             10044..11399
                     /gene="accC"
                     /locus_tag="O2692_001235"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCZ4298870.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            11414..11776
                     /locus_tag="O2692_001236"
     CDS             11414..11776
                     /locus_tag="O2692_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCZ4298871.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            11836..12225
                     /gene="nusB"
                     /locus_tag="O2692_001237"
     CDS             11836..12225
                     /gene="nusB"
                     /locus_tag="O2692_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCZ4298872.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKGHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            12242..13579
                     /locus_tag="O2692_001238"
     CDS             12242..13579
                     /locus_tag="O2692_001238"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCZ4298873.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            13572..13802
                     /locus_tag="O2692_001239"
     CDS             13572..13802
                     /locus_tag="O2692_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCZ4298874.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            13780..14661
                     /locus_tag="O2692_001240"
     CDS             13780..14661
                     /locus_tag="O2692_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCZ4298875.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDTTKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
     gene            15093..15545
                     /gene="argR"
                     /locus_tag="O2692_001241"
     CDS             15093..15545
                     /gene="argR"
                     /locus_tag="O2692_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCZ4298876.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            15561..17240
                     /gene="recN"
                     /locus_tag="O2692_001242"
     CDS             15561..17240
                     /gene="recN"
                     /locus_tag="O2692_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCZ4298877.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            17391..18812
                     /gene="lpdA"
                     /locus_tag="O2692_001243"
     CDS             17391..18812
                     /gene="lpdA"
                     /locus_tag="O2692_001243"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCZ4298878.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDTNEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            18828..19820
                     /locus_tag="O2692_001244"
     CDS             18828..19820
                     /locus_tag="O2692_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HCZ4298879.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEERETLKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            19820..20803
                     /locus_tag="O2692_001245"
     CDS             19820..20803
                     /locus_tag="O2692_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCZ4298880.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGL
                     QQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            20816..22090
                     /locus_tag="O2692_001246"
     CDS             20816..22090
                     /locus_tag="O2692_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCZ4298881.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTSTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTAEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIIKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            22441..22878
                     /locus_tag="O2692_001247"
     CDS             22441..22878
                     /locus_tag="O2692_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCZ4298882.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            22892..23872
                     /locus_tag="O2692_001248"
     CDS             22892..23872
                     /locus_tag="O2692_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCZ4298883.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(23999..24178)
                     /gene="prli42"
                     /locus_tag="O2692_001249"
     CDS             complement(23999..24178)
                     /gene="prli42"
                     /locus_tag="O2692_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HCZ4298884.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
BASE COUNT         8920 a         3309 c         4528 g         7524 t
ORIGIN      
        1 tgatgctaac agcaatttat ttttccattt tcacttttta tatcagtcaa tatagtttaa
       61 aattaaagac attatataat attgaaactg actataacta taagattgtt ttgttattga
      121 aagaagggaa aacgcatgaa tcatgataaa tcaccaataa ttttaattgg tttcatgggt
      181 accggtaaat ctacgattgg taaatacgtt gcagatgagc aaaatttatc atttattgat
      241 atagattcat atatcgaaga gaagtataag ttaacaatac cagaaatatt ttgtaaacat
      301 ggtgaacaat atttcaggaa tttagagttc acatgtttgc aagaatgtat taacactgca
      361 gatataattg ctactggtgg tggtattatt gagagtgaag aggcatttaa ttttttgaaa
      421 aatcaaaaaa acattatttg gttagattgt aatattgata ttatatatag tcgaatcaat
      481 gatgacccac atcgacctaa tgcaaataat aagacaatca agcagttaaa tgacttgtat
      541 tgctcgcgga atttaagata taatgaaatc gcattcaaga aatttgatag tcatttgcta
      601 tcaatttcag aaatatatta tgaattgcta aatttaataa aagcgagtga tcagtattag
      661 agagaataga gcgttaagac tctatcgccg aaggtgcaag taatttatta cgaaactctc
      721 aggcaaaagg ataatactgt aacgcgttcc tgaattggtg atttataaac agggtagcga
      781 ttgctatcct gtttttataa ttttaagggg gtatttcaat gtcaagtgat ttaaaacaaa
      841 cacctttata tcaaaattat gttgatagag gtgcaaaaat tgtggaattc ggaggatggg
      901 cgatgcctgt tcaattttca agtattaaag aggagcataa tgctgttcga tacgaaattg
      961 gcctgtttga tgttagtcat atgggtgaaa ttgaagtaac aggtaaagat gctagtcagt
     1021 ttgtgcaata tttattatca aatgatactg ataatttaac tacttcaaaa gcattatata
     1081 ctgctttatg taatgaagaa ggcggtatta ttgatgattt agtaatatat aaattagctg
     1141 acgacaatta tttattagtt gttaatgctg ctaatactga aaaagatttt aattggattt
     1201 taaaacacaa agagaaattt gatgttgaag tacaaaatgt atcaaaccaa tatggtcaat
     1261 tagcaataca aggaccaaaa gctagagatt taattaatca attagttgat gaagatgtaa
     1321 ctgaaatgaa aatgtttgaa tttaaacagg gtgtcaaatt atttggagca aacgtcattt
     1381 tatctcagtc aggttacaca ggtgaagatg ggtttgaaat ttattgtaat atcgatgata
     1441 ctgaaaaaat ttgggatggt ttattagagt ataatgttat gccatgtggg ttaggcgctc
     1501 gtgatacatt aagattagag gctggattgc cattacatgg acaagattta actgaatcaa
     1561 ttacaccata tgaaggtggt atcgcttttg caagtaaacc attaattgat gctgatttta
     1621 ttggtaaatc tgtattaaaa gatcaaaaag aaaatggtgc accaagaaga acagtgggat
     1681 tagaattact tgaaaaagga attgcaagaa ctggttatga agttatggat ttagatggaa
     1741 atattattgg agaagtaact tcaggaacac agtctccatc atcaggaaaa tcaattgcac
     1801 ttgcaatgat aaaaagagat gagtttgaaa tgggtagaga gttgcttgtt caagttcgta
     1861 agcgtcaatt aaaagcgaaa attgttaaga aaaatcaaat tgataaataa ttaaaaaggg
     1921 gtgtgcattg tgagtcatcg ttatatacct ttaactgaaa aagacaagca agaaatgtta
     1981 caaacaattg gtgcaaaatc tataggagaa ttattcggtg atgtaccaag tgacatttta
     2041 ttaaatagag atttaaatat tgctgaaggc gaagcggaaa caacgttact tagaagatta
     2101 aatcgcattg caagcaagaa tatcactaaa gaaacgcata catcattttt gggtgctggt
     2161 gtttatgatc attacgcgcc atcagttgtg gatgctatga tttcaagatc agaattttac
     2221 acagcatata cgccatatca accggaaatt tctcaaggtg aattacaagc catttttgag
     2281 ttccaaactt taatttgtga attaacagat atggatgtag caaattcttc tatgtatgat
     2341 gggatgacta gctttgctga agcatgtatc ttagcattta gtcaaacaaa gaaaaataaa
     2401 attgtagtct caaaaggttt acattatcaa gcattacaag tacttcatac ttatgctaag
     2461 acgcgtaagg aattcgaagt agtggaaatc gatttagatg gcactgttac agatttgaaa
     2521 aaattagaag cagcagtgga tgatgaaaca gcagctgttg cagtacaata tccaaacttt
     2581 tacggttcaa ttgaagatct tgaaaagatt catagcttta ttgaagataa aaaagcatta
     2641 tttatcgtat atgcaaatcc attagcattg ggattactta cacctccagg ttcatttggt
     2701 gcagatattg ttgtaggtga tactcagcca ttcggaattc ctgctcaatt cggtgggcca
     2761 cattgtggat attttgcaac gacgaaaaag ttaatgcgta aagtgccagg tagattagtc
     2821 ggtcaaacac aagatgatga aggtaatcgt ggctttgtat taacattaca agcacgtgaa
     2881 caacatattc gacgcgataa agcgacatct aatatttgtt ctaaccaagc attaaatgct
     2941 ttagcgtcat caattgcaat gtcagcactt ggtaaacaag gtatctatga tattgcagtt
     3001 caaaatatag aacatgctaa ctatgctaaa caacaattta ttaaaaaagg ttttgaagta
     3061 ttagatggta catctttcaa tgagtttgta gtcaaatttg acaaaccaat tcaacaagtt
     3121 aacgaggaat tagtgaagta caatattatc ggtggttttg atttaggtgt tgtttcagac
     3181 gactttaaaa atcacatgtt aatagctgta actgaattaa gaacaaaaga tgaaatcgat
     3241 acatttgtag aaaaggctgg tgagttaaat gactagtaaa tcaagtccat taatttttga
     3301 aagatctcgt gaaggcagat atgcatattc attaccaaaa agtgatatta aaacaaattc
     3361 tgttgagtca ttgttagatg ataaatttat tcgtaaaaac aaagcggagt ttcctgaagt
     3421 tgctgaacta gacttagtac gtcattatac tgaactttca aataaaaatt tcggtgtaga
     3481 caacggattt tatccattgg gttcatgtac gatgaagtat aaccctaaaa ttaatgaaaa
     3541 ggttgctaga ataccaggat ttagtgaatc acatccatta caagatgaag atcaagttca
     3601 aggttcatta gaaattattt atagtttgca agaagaatta aaagaaatta ctggtatgga
     3661 tgaggtgaca ttacaaccag ctgctggcgc acatggtgaa tggactgcat tgatgatatt
     3721 taaagcttac catgagaata atggtgaagg tcatcgtgat gaagtcattg tgccagattc
     3781 tgcgcatggt acgaatccag cctcagcttc atttgcagga tttaaatcag ttactgtaaa
     3841 atcaaacgaa cgtggcgaag ttgatattga tgacttgaaa cgtgttgtaa atgaaaatac
     3901 agcagctatt atgttaacta atccaaacac tttaggtatt ttcgaaaaaa atattatgga
     3961 aatccgtgaa atcgtccata atgctggtgg tctattatat tatgatggtg cgaatttaaa
     4021 cgctattatg gacaaagttc gcccaggaga tatgggattt gatgctgttc atttaaactt
     4081 gcataaaaca tttactggtc cacatggtgg tggcggtcct ggttcaggtc cagtcggtgt
     4141 agtaaaagaa ctagcaagtt acttaccaaa gccaatggtt attaaagatg gcgacaaatt
     4201 taaatatgat aatgacatta aaaattctat cggacgtgta aaaccatttt atggtaactt
     4261 tggtatttac ttaagagctt atacgtatat tcgaactatg ggagcaactg gacttaaaga
     4321 ggtttctgaa gcagcggttc ttaatgcgaa ttatattaaa gcacgtttat ctaaacactt
     4381 tgaaatacct tataaacaat attgtaaaca cgagtttgtg ttaagtggtg tgcgtcaaaa
     4441 agaatttggt gtacgtactt tagacatggc taagcgatta ttagatttcg gtgtacatcc
     4501 accaacaata tacttcccat taaatgttga agaaggtatg atgattgaac cgactgagac
     4561 agagtctaaa gaaacacttg attattttat cgatacatta attagtattg ctgaagaagc
     4621 taaaaatgat cctgataaag tgctagaagc accacataca actgtgattg atcgattaga
     4681 cgaagctaca gctgctcgta aaccaatatt aaagtttgaa aatcttaaac aggaaaaata
     4741 aagtattaaa cacatattcc gagaattatt attctaactt tgtatgaaga tttaaggata
     4801 atggtttcaa aatcaattga aaaagacaat ttctatttaa acaagaaaac taaaccgaag
     4861 taataactct tagggtttgg tattattctt tcatagaaat tgtcttttca ttttttagat
     4921 tgcggtaatt gaatcgtatt gaaaatgagc tgaactttct ttattatgct gaaactaagt
     4981 ttaatgatca gtgaagctag agaaatatag taacagactt tgttaaaaaa ttaaaatctg
     5041 agtattgtta ttgatgatca attaacactc agattttatt aaatcgtata ttaaatttac
     5101 aaaaactatt ttttagactt tatttttcca gtccattttt tatagccgcc ttttaacata
     5161 tagatatctg tatatccatt ctttttcaaa atacgagcgg ctctatagct agcaatccca
     5221 ttggcatcac ataagtatac cggttgatct tttcttaatc cttggaatcg ttgcctgaac
     5281 attgtcatag gaatattgcg agacccatta atgtgaccgt agtcatagtc aactttctct
     5341 ctaacatcga tgacttgagc ttttctaatc ccattatgga attcattttg atctaattct
     5401 ttaacagctc gcttgttaag aatttgttga acaatcatat aagcaataat tgctataact
     5461 aaaattattg cgatgtacaa actagcactc atcttgcatc ctccctaatt aaacgataat
     5521 ataattataa gactgttagg tcaaattatc aaaatatttc actaaagaat tgcaattatt
     5581 attcatttag ttaaattgat taattagcga atcaaatatt atacattttg ctgataggag
     5641 ttcttgaata tgactgaaac ttggaatttt attaatacag gaagtaaaga cccatattat
     5701 aatatggcaa tggatgaagc gttattaaat tttgtctcta gaggtgaaat tgatccagtt
     5761 atacgttttt acacatggaa tcctgcaaca ttatcaatag ggtatttcca aaggctacaa
     5821 aaagaaattg atattgataa ggttaaagag aaaggttttg gtctcgtaag gcgtcaaact
     5881 ggtggccgtg gggtgctaca tgataaagag ttaacttaca gtgtgattgt gcctgaatcg
     5941 catccgaata tgccttctac agttacagaa gcgtacagag tgatttcaca aggattatta
     6001 gaaggattta aaaacttagg atttgatact tattttgcgg tacctaaaac gcctgaagag
     6061 cgtcaaaaat taaaacaacc tcgaagttca gtgtgttttg atgcaccaag ttggtatgag
     6121 ctagttgttg aaggccgtaa gattgctggg agtgctcaaa cgagacaaaa gggcgtaatc
     6181 ctccaacacg gttcgatttt acaagatatt gatattgatg aactgtttga tatgtttatt
     6241 tataaaaatg aaagattaaa attaaaaatg aaagaggcat tcgttgaaaa agcagttgcc
     6301 atcaatgata tttcagatga acacattact attagtcaaa tggaagaagc tttcgagaaa
     6361 ggttttaaga aaggtttaaa tatagaattt aagccacttg aattaacaga agctcaattg
     6421 gcagaagtgg aagaattaac tgaaaaatat cgttcagatg aatggatgtt tagaaagtag
     6481 tttttagaat aaaaaatgga agcacgtaga ctattttacc tacaagtgct tccgtttttt
     6541 tatctatttt cttctttgat ttcttttata cttacgcatt gctttgcgat atttgcgttg
     6601 gtcttcagtt aagatgaaga aataataaat cagataaata ataccagcta ttacagcaaa
     6661 gctgattaac attctgacga ttgaaaaaat aaaggcatct agattcatta ctaatccaat
     6721 cgcagcaata ataagtacaa gataaaatat tatattacgc attaaaagac tcctatcatg
     6781 attttgcatt taagtttaat tttgacaacg cttgttgtcc ttttgtaagt tgttttttat
     6841 ctttattttg atagccattt ttaatatcat caaatgcatg attcaaatca gagtttaatt
     6901 tatcgatatt tttagacgtt tttttatcat catcactaag tgataaacca tcgatagcat
     6961 ctttgtatga agtgtgagca ctttgaatat cattagaaat ttgatttaat ttcgattgaa
     7021 cattcttatc gcctttgttt ttacttacat ctttctcaat atcgttatag gatttcaaag
     7081 ctttagcaac tttgtcgtag tatgttgatg taacattcat caatttaatt ttcgtattat
     7141 tagatgcagt ttttttactg ttttctttat ccttttcaag ccctttaatt ttagatttag
     7201 aatcactaat ttgttgatta agttcttgaa tatctagttt gagttgatga ttatcttctc
     7261 ttaaatctgt tgttttttct tctaatggtg ctaaattttg tgatccacat ccagctaaca
     7321 ataatgttgc gccaacaatt gaaaccaatt ttttcattac tatctcccta aaatcaatat
     7381 ctattatttt ttatccttat tatgatatta ttttaatgta taaagttgaa atgcacaaac
     7441 gtttcactag gaggtaataa gatgagcaga ataacacaag tgcatcgtat tttagaacaa
     7501 aaacatttag atgcgattat cattctttcg gattacaata gaagatattt atcaggtttt
     7561 actggtacca gtggagcact tatcatatct aaagataaac aatatttgat aacagacttt
     7621 agatatattg atcaagcaac taagcaggcg ccaaattatg aaattattaa tcgtaaatct
     7681 actattattg gtgagattaa agaattgcta caccaagaaa attttgaaaa tgtaggtttt
     7741 gaggggcatc atgtaagtta tgatacatac cttgaattaa ataaaagccg tatatcatta
     7801 ataagcattt ctaatactgt agataaaatt agagacgtca aagatgctga cgaaattgct
     7861 ttaattcaaa aagcagctaa tattgttgat gaaacatatg aatatatttt aactgttgta
     7921 aaagcaggca tgactgaaaa agaattaaag gcaatattag aaagcaaaat gctagaatta
     7981 ggagcagatg gaccatcatt cgatacgatt gtagcatctg gtcatagagg tgcattacca
     8041 catggtgttg caagtgataa aattattgaa aaaggcgaca tgattacatt agattttggc
     8101 gcgtattata acggctattg ttcagatatt actagaacat ttgctattgg agaaccagat
     8161 cctaaactga aagaaatata tcaaatagta cttgaatctc aaatgaaagc aattaatgag
     8221 attagacctg gcatgactgg tgcagaagct gatgccattt caagaaacta tttagagtca
     8281 aaagggtatg gaaaagaatt tggacattca ctaggacatg gtattggttt agaaatccat
     8341 gaagggccaa tgctggctcg tacgatacaa gataaacttc aagttaacaa ctgtgttaca
     8401 gtagaacctg gtgtttatat agaaggtttg ggcggtataa gaatagaaga tgatatttta
     8461 attacagaaa atggttgtca agtctttact aaatgcacaa aagaccttat agttttaaca
     8521 taagcgtgta aaatgaggag gaaactgaat gatttcggtt aatgatttta aaacaggttt
     8581 aacaatttct gttgataacg ctatttggaa agttatagac ttccaacatg taaagcctgg
     8641 taaaggttca gcattcgttc gttcaaaatt acgtaattta agaactggtg caattcaaga
     8701 gaaaacgttt agagctggtg aaaaagttga accagcaatg attgaaaatc gtcgcatgca
     8761 atatttatat gctgacggag ataatcatgt atttatggat aatgaaagct ttgaacaaac
     8821 agaactttca agtgattact taaaagaaga attgaattac ttaaaagaag gtatggaagt
     8881 acaaattcaa acatacgaag gtgaaactat cggtgttgaa ttacctaaaa ctgttgaatt
     8941 aacagtaact gaaacagaac ctggtattaa aggtgatact gcaactggtg ccactaaatc
     9001 ggcaactgtt gaaactggtt atacattaaa tgtaccttta tttgtaaacg aaggtgacgt
     9061 tttaattatc aacactggtg atggaagcta catttcaaga ggataatctc taatttgtta
     9121 acaaatagct tgtattcact atactgattt aacgtaagat attctaaata agtctcataa
     9181 agctattgcc taaaatgatt ataggttata tgctgatatg aggcttttta tttttaaaat
     9241 aatttttaaa atataaaacg aatcgtctaa ttggtagtga aaaaacatag acatgagtag
     9301 aggtatatat ctacatatca tattgttgag gataaaacat aatagctaat aaaaataaca
     9361 ttgatatgtt taactgaatg cataaaattt ttaatcaaga gttaagatga gtcaatgtta
     9421 agtaataaat acacaagtgt ttttaattta aaatgtggtt ttattataac tttgggatag
     9481 acttataatg tcgccagcaa tatgaacatg cttgaattga agagttgtct caagtaaaat
     9541 agacgggtag atgaaaacaa actgaaggag tcagtaataa tgaactttaa agaaatcaaa
     9601 gaattaattg aaattctgga taaatcaact ttaacggaaa tcaatattga agatactaaa
     9661 ggcaaagtga cgcttaagaa agaaaaagaa actgagatta tcacgccaca aatctcacaa
     9721 atgccagttg aagctgcggc aatgcctatg cctcaagcac aatcaactga tagcaataaa
     9781 actgaagctc caaagccaac ttcagataat cacaaaacaa ttaatgcacc tatggtaggt
     9841 acattttaca aatcgccatc tccagacgaa gaagcatatg tgcaagttgg ggacactgtt
     9901 tcaaatgaaa caacagtgtg tattttagag gcaatgaaac tatttaatga aattcaagca
     9961 gaaatttcag gtgaaattgt tgaaatctta gtagaagacg gacaaatggt agagtatggc
    10021 caaccgttat ttaaggtgaa ataatgaaaa aggttttaat tgcaaaccgc ggtgaaatcg
    10081 cagttaggat tattcgcgct tgtcgtgatt taggcatcca aactgttgca atctattctg
    10141 aaggggataa agatgcgcta catactcaaa ttgctgatga agcatattgc gtaggtccca
    10201 ctttgtctaa agattcatat ttaaatattc cgaacatctt atctattgca acttctacag
    10261 gttgtgatgg cgttcatccg ggttatggct ttttagctga aaatgctgat tttgcagaat
    10321 tatgcgaagc atgccaattg aagttcattg gaccaagtta tcaatctatc caaaaaatgg
    10381 gtatcaaaga tgttgctaag gcagaaatga tcaaagccaa tgttccagtt gttcctggta
    10441 gtgacggttt aatgaaagac gtctcagaag ctaagaaaat cgccaaaaaa attggctatc
    10501 cggtcatcat taaagctact gctggcggtg gcggaaaagg tatccgtgtt gctcgtgatg
    10561 aaaaagaact tgaaactggc ttccgaatga cagaacaaga agctcaaact gcatttggta
    10621 atggtggact ttatatggag aaattcatcg aaaacttccg ccatattgaa atccaaattg
    10681 ttggggacag ctatggtaat gtaattcatt taggagaacg tgattgtaca attcaaagac
    10741 gtatgcagaa attagtggaa gaagcacctt ccccaatttt agatgatgaa acacgtcgtg
    10801 aaatgggaaa tgccgcagtt cgtgcagcga aagctgtaaa ttatgaaaat gcgggaacaa
    10861 ttgagtttat atatgattta aatgataata aattttattt tatggaaatg aatacacgta
    10921 ttcaagtaga acatcctgta actgaaatgg taacaggaat tgatttagtt aaattacaat
    10981 tacaagttgc tatgggtgac gtgttaccgt ataaacaaga agatattaaa ttaacaggac
    11041 acgcaattga atttagaatt aatgctgaaa atccttacaa gaactttatg ccatcaccag
    11101 gtaaaattga gcaatatctt gcaccaggtg gatatggtgt tcgaatagag tcagcatgtt
    11161 atactaatta tacgataccg ccatattatg attcgatggt agcgaaatta atcatacatg
    11221 aaccgacacg agatgaagcg attatggctg gcattcgtgc actaagtgaa tttgtggttc
    11281 ttggtattga tacaactatt ccattccata ttaaattatt gaataacgat atatttagaa
    11341 gcggtaaatt taatacaaac tttttagagc aaaatagcat tatgaatgat gaaggttaat
    11401 aggaggtcaa tccatggtca aagtaactga ttattcaaat tcaaaattag gtaaagtaga
    11461 aatagcgcca gaagtgctat ctgttattgc aagtatagct acttcggaag tcgaaggcat
    11521 cactggccat tttgctgaat taaaagaaac aaatttagaa aaagttagtc gtaaaaattt
    11581 aagccgtgat ttaaaaatcg agagtaaaga agatggcata tatatagatg tatattgtgc
    11641 attaaaacat ggtgttaata tttcaaaaac tgcaaacaaa attcaaacgt caatttttaa
    11701 ttcaatttct aatatgacag cgatagaacc taagcaaatt aatattcaca ttacacaaat
    11761 cgttattgaa aagtaatgtc atacctaatt cagtaattaa ataaagaaaa atacaaacgt
    11821 ttgaaggagt taaaaatgag tcgtaaagaa tcccgagtgc aagcttttca aactttattt
    11881 caattagaaa tgaaggacag tgatttaacg ataaatgaag cgataagctt tattaaagac
    11941 gataatccag atttagactt cgaatttatt cattggctag tttctggcgt taaaggtcac
    12001 gaacctgtat tagacgagac aattagtcct tatttaaaag attggactat tgcacgttta
    12061 ttaaaaacgg atcgtattat tttaagaatg gcaacatatg aaatattaca cagtgataca
    12121 cctgctaaag tcgtaatgaa tgaagcagtt gaattaacaa aacaattcag tgatgatgat
    12181 cattataaat ttataaatgg tgtattgagt aatataaaaa aataaaattg agtgatgtta
    12241 tatgtcagat tatttaagtg tttcagcttt aacgaaatat attaaatata aatttgatca
    12301 agatccacat ctccaatctg tattgattaa aggtgagctt tcaaatttca aaaagcattc
    12361 aagtggtcat ttatacttta atgtgaaaga taaagaaagc gttataagtg ccatgatgtt
    12421 caaaggtagt gcttctaaat taaacttcga acctaaagaa ggagatgaag tcttattaga
    12481 agcacgtgtt tctgtttttg aacgtcgtgg aaactatcaa atttatgtaa ataaaatgca
    12541 attagatggt atagggaact tatatcaaaa attagaagca ttaaagaaaa aattaactga
    12601 agaaggttgt tttgataaag cgaataagaa atcaatacct aagtttccta aaaaaattgc
    12661 agttttaaca gcgagtacag gtgccgcaat tcgagatatc cattcaacga taaatagtcg
    12721 ttttccatta gctgaacaaa tacaaattag tactttggtt caaggtgaaa aagcaaaaga
    12781 cgacattatt gaaaaaattg aatatgcaga tagtttaggt gtagatacca ttattgtagg
    12841 tcgaggcggt ggatccatag aagatttatg gaatttcaac gaagaagctg tcgtccgtgc
    12901 aatatataat tgtaagacac cgattatatc agcagttggt catgaaacag actttacatt
    12961 aagtgatttt gctgcagaca tcagagctgc gactccaact caagctgctg ttattgcaac
    13021 accagaccaa tatgaattgc tgcaacaaat acagcaatat cagtttacat tgactcgttt
    13081 catcaagaaa catttagaac aacaacgtaa acatgttgaa catttgtcat cgtattacaa
    13141 gtttaaacaa ccaactttat tgtatgatca acagatacaa cgtcgagatg atttagaaaa
    13201 gagactgaaa caacaaattc aggcaacttt tgaacaacag agacatcgat taatgctgtt
    13261 gcaacaacgt tataatttaa aagcattact aagtagtgtt aatcaagaac aacaaaataa
    13321 tcttcaattg acaaaccaac ttgttaaatt attgaattct aagatattaa gttataaaaa
    13381 tgatttgaaa aataaagtag aaaacttaaa caatttaagt ccaactaata caatgttgcg
    13441 tggatatgca attgttaata aaaaagacga agtcattacg agtactaaag atttaacaga
    13501 aaatgatcaa ttgacattaa caatgaaaga tggcttagta gatgcaaaag ttacgaaagt
    13561 aaggtgtaat aatgactaaa gaaacgcaaa gttttgaaga aatgatgcaa gaattagagc
    13621 aaattgttca aaaattagat aatgaaacag tatctttaga ggaatcatta gatttatatc
    13681 aacgtggtat gaaactatca gcagcttgtg acacaacttt aaaaaatgcc gaaaaaaagg
    13741 tgaatgactt aataaaagaa gaagctgagg atgtaaaaaa tgacgaatct accgatgaat
    13801 aaattaatag atgaagtcaa taatgaatta tcggttgcga taaataaatc agtaatggat
    13861 actcagctag aagaaagtat gttgtattca ttaaatgctg gaggtaaacg catccgacca
    13921 gttctgttat tactcacttt agattcacta aataccgagt atgagttagg tatgaagagc
    13981 gcaattgcac tagaaatgat tcatacatat tcacttattc atgatgacct accagcgatg
    14041 gataatgatg attatcgacg aggaaaatta acaaatcata aagtatatgg tgagtggact
    14101 gcgatattag caggtgatgc tttattaact aaagcatttg aacttatttc aagtgatgat
    14161 agattaactg atgaagtaaa aataaaagtt ctacaacggc tgtcaatagc aagtggtcat
    14221 gttggaatgg tcggcggtca aatgttagat atgcaaagcg aaggccaacc aattgatctt
    14281 gaaactttgg aaatgataca caaaacaaaa acaggagcat tattaacttt tgcggttatg
    14341 agtgcagcag atatcgctaa tgtcgatgat acaactaaag aacatttaga aagttatagt
    14401 tatcatttag gtatgatgtt ccagattaaa gatgatttat tagactgcta tggtgatgaa
    14461 gcaaagttag gtaaaaaagt gggcagcgat cttgaaaata ataaaagtac gtacgtgagt
    14521 ttattaggga aagatggcgc agaagataaa ttgacttatc atagagacgc agcagtggat
    14581 gaactaacgc aaattgatga acaattcaat acaaaacact tattagaaat cgttgattta
    14641 ttttatagca gggatcacta aaagcaaata tatcgattag caacaattgt aaagtttgaa
    14701 atgtctcaaa aggcatattc aactgacgtt gtttgttaat cgatttttct catgttgaaa
    14761 cttaaaatta tgttgaagac tttcatttaa aaaatagaat tttcaatttc tatacgacaa
    14821 tattcgtatc tgtgatatca atgtgatttt aattaaatat gttcaaacaa ttgacgctta
    14881 ttaaatctaa taattagcaa gtttattaca catatattca tttttataca tattttaagc
    14941 aattgttatg cgtaatatga aaaatattta taaatagata accaagatag ataaaccatg
    15001 tttacccttt ttacgtaaag gtgtttagta gtcgtaaatc agtgcatgtg ttaaaataaa
    15061 tgtataaata ttcgtaatca gaggtgttta ctgtgcccaa aaaatcggtt aggcatataa
    15121 aaattagaga aattatttca aatgaacaga tagagacaca agatgaatta gttaaacgat
    15181 taaacgatta tgatttaaat gtcactcaag caactgtttc tcgtgatatt aaagaactac
    15241 aacttattaa agtacctata ccttcaggtc aatatgttta tagtttacca aatgatagaa
    15301 aattccatcc tttagaaaaa ttgggacgtt atttaatgga ttcctttgtt aatatagatg
    15361 gtactgataa tttacttgtt ctaaaaacat tacctggtaa tgcacaatct attggagcta
    15421 tattagacca aatcaattgg gaagaagtac taggcacaat ttgtggtgat gatacttgtt
    15481 taattatttg tcgaagcaaa gaggcaagtg atgaaatcaa gtcaagaatt ttcaatttgt
    15541 tataaggatg cgatgagttt atgttacaaa ccttatcaat caagcaattt gctattattg
    15601 aagaattaga aattcaattt tctgatggtt taactgtttt aagtggtgaa acgggttcag
    15661 gtaaatcaat tattatagat gcaattggac aattaattgg aatgagagct tcatctgatt
    15721 ttgtaagaca tggtgaaaaa aaggctgtca tcgaaggtat atttgatatt gacgagagta
    15781 aagatgctat acatatattg aaaaatatgg atattgatgt agatgaagat tttttattag
    15841 ttaaacggga aattttcagc tctggtaaaa gtctttgtaa aattaataat caaacagtta
    15901 ctttacaaga tttacgaaaa gttatgcaag aattattaga tattcatggc caacatgaaa
    15961 cgcagtcttt gttgaaacaa aaataccatt taacattatt ggataattac gcagaatcac
    16021 gttatcaaga tttgttggat aaatatcacc aaacttttca aaattataaa gccaaaaagc
    16081 aagagttaga agatttagaa tcagcagacc aagcattgct acagcgttta gatttaatga
    16141 aattccaatt agaagaattg tctgaagcac atctaaaaga aggcgaaata gagcaacttg
    16201 aaatagatat taaacgcatt caaaactctg aaaaattaag cttagcgctt aataacgcac
    16261 atatgacatt aacggatgaa aatgctatca ctgatcgttt atatgaacta agcaaccatt
    16321 tgttaacaat aaacgatata gttccaaaca aatatgacaa attaaaagaa gatattgatc
    16381 aattttacta cattttagaa gatgcaaaac atgagttata tgatgaaatg gctaatacag
    16441 aattcgacga acaagtgtta aacgagtatg aatctcgtat gaatttgctg aataatttaa
    16501 aacgtaaata tggaaaagat atttcagaat taatcgcata tcaagaaaag cttaataatg
    16561 aaatcaataa aattgaaaac tatgaacaaa gtacatcgca gttacgagaa gaaataaatg
    16621 cattgtataa tcaagttata gaggttggac aagcgttatc aaagcagcgt cgtatagtcg
    16681 ctagggagtt aagagaccac attgtatcgg aaattcaaaa cttacaaatg aaagacgcaa
    16741 atcttgagat ttcatttaaa aaattagaag aaccgaatat tgatggaatc gaatttgtag
    16801 aatttttaat cagtccaaat aaaggggaac cattaaaaag tttaaataaa attgcgtcag
    16861 gtggagaact ttctagaatt atgttagcgt taaaaagtat ttttgttaaa tcgagaggtc
    16921 aaactgcaat tttattcgat gaggttgact caggtgtatc tggacaagct gcacaaaaaa
    16981 tggcagagaa aatgcgagat atagcagaat atatacaagt tatttgtatc tctcacttac
    17041 cacaagttgc atcgatgagc gaccatcatt tattaatttc gaaatcatca aaagatgatc
    17101 gtacaacaac acaagttcaa gagttaatta gcgatgataa agtagatgaa attgcacgaa
    17161 tgatttcagg tgcaagtgtt actgatttaa ctcgagaaaa tgcaagagaa atgatacaac
    17221 acaatcaaag acgtagataa tttccaaagt gtgttgaata ctcatttatg gatttattta
    17281 tgtgcattca aactaaatag acaatcatgt aaaataggta attaatggac atgaaaacta
    17341 tgtattaata aattttcaat ggatatatat cattagttag gagtaagaac atgtcagaga
    17401 aacaatatga tttagtcgtt ctcggtggag gtactgcagg atatgttgct gcgatacgtg
    17461 cgtctcaatt aggtaaaaaa gtggcaatag tggaacgaca actattagga ggtacttgtc
    17521 tccataaagg ttgtattcct actaaatcgc tgcttaaatc tgctgaagta tttcaaacag
    17581 taaaacaggc agcaatgttt ggcgttgatg tcaaagatgc taatgttaat tttgaaaata
    17641 tgttggcacg aaaagaagac attattaatc aaatgtatca aggtgtaaag catttaatgc
    17701 aacacaatca cattgacatt tataatggca cgggacgtat tctaggtaca tctatatttt
    17761 cacctcaaag tggcacaatt tctgtcgaat atgaagatgg tgaatcagac ttattaccca
    17821 accaatttgt attaattgca actggttctt cgcctgcaga gttgccattc ttatcatttg
    17881 atcatgataa aattttatct agcgatgata tattatcatt aaagacgtta ccatcaagta
    17941 ttggtattat tggtggtggt gttatcggaa tggaatttgc atcattgatg atagatttag
    18001 gtgttgacgt aacagttata gaagcaggtg aaagaatttt accaactgaa agtaaacaag
    18061 cttcacaact attaaaaaaa tcattgtcag cacgtggtgt taaattctat gagggaataa
    18121 aactttctga aaatgatata aatgttaacg aagatggtgt tacgtttgaa atttcctcag
    18181 acataattaa agtagataaa gtgttgctat cgattggtag aaaacctaac acatcagata
    18241 ttggtttaaa caacactaaa ataaaacttt caacatcagg acatatttta acgaacgaat
    18301 ttcaacaaac tgaagataaa catatttatg cagctggtga ttgtatagga aaattacaat
    18361 tggcacatgt tggatcaaaa gaaggtgttg tggctgttga tcatatgttt gaggggaacc
    18421 caatcccagt aaactataac atgatgccta agtgtattta ttcacaacct gaaattgctt
    18481 ctatcggttt aaacattgaa caagcaaagg cagagggaat gaaagttaaa agttttaaag
    18541 taccatttaa agcaattggt aaagcagtga ttgatagcca tgacacaaac gaagggtata
    18601 gcgaaatggt gattgatcaa tcaactgaag aaattgtggg tattaatatg attggtccac
    18661 atgtaacaga attgattaat gaggcatcac tgttacagtt catgaatggc tcggcattag
    18721 aattaggact aacaacacac gcacatcctt ccatctctga agtgttgatg gaattaggat
    18781 tgaaagcaga aagtagagct attcacgtat aaataggagg atttaacatg attgattata
    18841 aatcattagg ccttagcgaa gaagacctaa aagtaatata taaatggatg gatttaggta
    18901 gaaaaataga tgaaagactg tggttattga accgtgcagg taaaattcca tttgttgtaa
    18961 gtggtcaagg acaagaagca actcagattg ggatggcata cgcattagaa gaaggtgata
    19021 ttacagcgcc ttattataga gatctagctt tcgttactta tatgggtatc tcagcgtatg
    19081 atacgttttt atcagcgttt ggaaagaaag acgatgttaa ctctggtgga aaacaaatgc
    19141 catcacactt tagtagtaga gctaagaata ttttgtctca aagttcaccg gtagcaactc
    19201 aaatacccca tgctgttggt gctgctttag ctttaaaaat ggatggtaag aagaaaattg
    19261 caacagcaac tgttggtgaa ggtagctcaa accaaggtga cttccatgaa ggtttaaact
    19321 tcgcgggtgt acacaaactt ccttttgtat gtgtcattat aaataataaa tatgcaattt
    19381 cagtacctga ttcattacaa tacgcagctg aaaaattatc ggatagagca cttggttacg
    19441 gtatacatgg tgaacaagta gatggtaatg atccattagc aatgtataaa gcgatgaaag
    19501 aagcaagaga tcgagctatt tctggtcaag gttcaacttt gattgaagca gtaacaagtc
    19561 gtatgacagc tcattcatca gatgatgacg accaatatcg tacgaaagaa gagcgtgaaa
    19621 cgcttaaaaa agcagactgc aatgaaaagt tcaaaaaaga gttgctttca gctggcatta
    19681 tcgatgatgc ttggttggca gaaatagaag cggagcataa agacatcatc aataaagcga
    19741 caaaggctgc tgaagacgcg ccatatccaa gtgtcgaaga agcctatgca tttgtgtacg
    19801 aagaagggag tcttaactaa tggctaaatt atcttattta gaggcgatac gccaggcaca
    19861 agacttagca ttacaacaaa ataaagatgt gtttattctt ggtgaagatg ttggtagaaa
    19921 aggtggcgta tttggtacta ctcaaggact gcaacaaaaa tatggtgaag acagagttat
    19981 cgatacgcca ttagctgagt caaatattgt tggaactgcc attggtgcag ctatggtagg
    20041 taaaagacca attgcagaaa ttcaatttgc agattttatt ttacctgcga caaatcaaat
    20101 tattagtgaa gctgcgaaaa tgcgttatcg ttcaaataat gattggcaat gcccattaac
    20161 gattcgtgca ccatttggtg gcggtgttca cggaggacta tatcattcac aaagtattga
    20221 aagtatattt gcttcatcgc caggtttaac aattgttatt ccttccacac cgtatgatgc
    20281 taaagggctt ttattatcat ctatcgaatc aaatgatcca gtattgtact ttgagcataa
    20341 aaaagcttat cgtttcttaa aggaagaagt gccagaagaa tattacactg tacctttagg
    20401 aaaagcagat gtgaaacgtg aaggtgaaga tttaacagta ttttgttatg gattaatggt
    20461 caattattgt ttacaagcag cggatatttt agctgcagac ggtatcaatg ttgaagtcgt
    20521 tgatttacga actgtttatc cattagataa agaaacaatt attgatcgtg ctaaacatac
    20581 aggtaaagtt ttacttgtaa ctgaagataa tttagaaggt agtattatgt cagaagtgtc
    20641 agcgattatt gcagagcatt gcttgttcga tttagatgca ccaatcatgc gtttagctgc
    20701 tccagatgta ccatctatgc cattttctcc tgtattagaa aatgaaatta tgatgaatcc
    20761 agaaaaaatc ttaaataaaa tgcgtgaatt agcagaattc tagggaggga aagtcatgga
    20821 aataacaatg cctaagttag gtgagagtgt tcatgaaggc accattgaac aatggttagt
    20881 ttctgttggt gatcatattg atgaatatga accattatgt gaagttatta cagataaagt
    20941 gacagctgaa gtcccttcca cgatatcagg aacaattaca gaaattttag ttgaagcggg
    21001 gcagacagta gctattgata caattatctg taaaattgaa actgctgatg aaaagacaaa
    21061 tgaaacaact gaagagatac aagcaaaagt ggatgagcat actcagaaat ctactaaaaa
    21121 agctagtgca acagtggaac agacatctac tgctaaacaa aatcaaccac gtaataatgg
    21181 tcgcttttca cctgttgtat ttaaactcgc ttcagagcat gacattgatt tatcacaagt
    21241 tgtaggtagt ggatttgaag gtcgtgtaac taagaaggat ataatgtcag ttattgaaaa
    21301 tggtggtacc acagctcaat ctgacaaaca agttcaaaca aaatcaacat cagtagatac
    21361 atcaagtaac caatcatctg aagacaatag tgaaaacagc acaataccag taaatggtgt
    21421 gcgtaaagca attgcgcaaa atatggttaa tagtgtaaca gagattccac atgcatggat
    21481 gatgattgaa gtagatgcta caaatcttgt gaatacgaga aatcattata aaaacagctt
    21541 taaaaataaa gaaggatata atctaacgtt ctttgctttc tttgtaaaag ctgtagcaga
    21601 tgctttaaaa gcatatcctt tattaaatag tagctggcaa ggaaatgaaa ttgtcttaca
    21661 taaagacatt aatatttcaa ttgctgttgc tgatgaaaat aaattatacg tacctgtgat
    21721 taagcatgca gacgaaaagt caatcaaagg tatagctaga gaaattaata ctttagcaac
    21781 gaaagcgcgt aataagcaat tgacagctga agatatgcag ggcggtacat ttacggtaaa
    21841 taatactggt acatttggtt cagtatcatc aatgggtatt ataaatcatc cacaagcagc
    21901 gattttacaa gtagaatcaa tcattaaaaa gccagtagta attaatgata tgattgcaat
    21961 tcgtaacatg gttaatttat gtatttcaat tgatcatcgt attttagatg gtttacaaac
    22021 aggtaaattt atgaatcata ttaaacagcg tatcgaacag tatactttag aaaatacaaa
    22081 tatatattag tgataacata gatgcatcta tcgacaactt gttttatctt gttcttgtcg
    22141 atggatgtat tattttttgg cactaaaata tgtgcaatat attcaaaaaa gataaagaac
    22201 aataatcaac atggttgaat gcatttttgc agtaaagtca aaataagaca tcatacttga
    22261 aacatattaa tgaaaacatg tgaacaaatt agttaccatg attttaagca caataatgtt
    22321 tggtatattg ttaaaattgt gtctaaatat aggtgtgatt cagattagtt tattgaacaa
    22381 tatgttatta attagtagaa tgaggatagt ttaaatataa agggataggt gattgaactt
    22441 atggacatga atttcgattt atacatgaac ggtgttgtag aacaagcaag gaatgaaatt
    22501 gaatctgcgg gatatgagca attaactact gcagaagatg ttgacaaagt tcttaaacaa
    22561 gatggtacaa cactagttat gatcaattct gtatgtggtt gtgcaggtgg tatcgcaaga
    22621 ccagcagcat cacatgcttt acattatgac gtattacctg atcgtctagt gacagtattt
    22681 gctggacaag ataaagaagc gacacaaaga gcgcgtgaat acttcgaagg ttatgcgcct
    22741 tcaagtccgt catttgcatt agtaaaagat ggaaagatta cagaaatgat tgaaagacat
    22801 caaatcgaag gtcatgatgt gatgaacgta attaatcaat tacaaacatt attcaataaa
    22861 tattgtgaag aaagataaga ggcgctaacc catgttaaag ttaaatcctt acaagattgg
    22921 atttagaaca ataaaaacag cagtgggtat gactttaggt gtaattatta gtaagctgtt
    22981 aggtttagat aattatgctt caagcgccat attagtcgta ttatgtatta aacatacaaa
    23041 agtacattcg ctacaagcga ttatttcaag attagtatca tgttttttag tattgttttt
    23101 aggttcagca atatttagtt tattaggtca gagtccaatt gtactcggta ttatcgtatt
    23161 gttatttata ccattaactg tcgtattaaa agtacaagaa ggtgtcatta cgagttgcgt
    23221 tatattactt catgttttta atgcaaaatc aattgatgca catttaattg ttaatgaaac
    23281 attattactg ttaattggac taagcattgc atttacaatg aatttaatga tgccaagttt
    23341 agacaaacaa ctagacgaat acaaatgtaa aattgagcaa caaattgctg atatttttag
    23401 taaatatagt tatatttgtg aaaaatatga agataccatt gcgattgaat ttgaagtgtt
    23461 acttttaaat attaaaaagg cgaagtctat cgcgttccga gatgttaaaa atcattttgt
    23521 tagaaacgaa aattcatact atcattattt tgatatgcga gaagagcaag tggaattgtt
    23581 aatgagaatg aaaccgctca tcgaaagtat ctgtcataaa gatccgcttt taaatgatgt
    23641 tgcgtcactg ctgaaagaaa ttggtctaaa tgtgaatagt aatgactata cagcaatgcg
    23701 tttacacaat ttgtatgaga cgcgtttgaa attggatgaa ctgccattgc caacaacaca
    23761 caaagcgctt aactcgagag caagtattat tcaaatatta aatgaattag aagaatattt
    23821 acaaatcaaa tcgcaatttg gttcattgaa aatgcatagt gaagtagctt aaaaaataat
    23881 ccatccgtat gagccatgtt tgaaagtaat cgttgcaata cgataactta ttaatacatg
    23941 cgtttataat ggatggattt ttaattgtat gattttgtat aggtagttga gatatagttt
    24001 ataaaattgg aatgatactc gttaataaca atgctaatac aactgataca agcataacga
    24061 taatgataat tcttaaaact ttattttgca ttttgtgtgc ctccaaatga ataacctttg
    24121 tattaataat tacgacataa gatataaaac ttttaacttg agacgatata actagcaatc
    24181 atcataaagg aatataataa aaaacatgag taagtttgct tatgtcattt tcctcattat
    24241 acgatatctt tgaaaaatca atcaatatat ttttgacaaa g
//
LOCUS       DALAGF010000034        23988 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.034, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000034 DALAGF010000000
VERSION     DALAGF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23988
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.034"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            59..160
                     /locus_tag="O2692_001250"
     CDS             59..160
                     /locus_tag="O2692_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298885.1"
                     /translation="MENFDVGGGNFTFMEIGIGFMTHKPKRLNDDID"
     gene            complement(245..928)
                     /locus_tag="O2692_001251"
     CDS             complement(245..928)
                     /locus_tag="O2692_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HCZ4298886.1"
                     /translation="MKFTALAKATLALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            complement(1294..1992)
                     /locus_tag="O2692_001252"
     CDS             complement(1294..1992)
                     /locus_tag="O2692_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HCZ4298887.1"
                     /translation="MKLTTIAKATLALGILTTGVFTAESQTGHAKVELDETQRKYYIN
                     MLHQYYSEESFEPTNISVKSEDYYGSNVLNFKQRNKAFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKNGFSINELFFIQK
                     EEVSLKELDFKIRKLLIEKYRLYKGTSDKGRIVINMKDEKKHEIDLSEKLSFERMFDV
                     MDSKQIKNIEVNLN"
     gene            complement(2372..3070)
                     /locus_tag="O2692_001253"
     CDS             complement(2372..3070)
                     /locus_tag="O2692_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HCZ4298888.1"
                     /translation="MKFTVIAKAIFILGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGSADKGRIVINMKDENKYEIDLSDKLDFERMADV
                     INSEQIKNIEVNLK"
     gene            complement(3414..4109)
                     /locus_tag="O2692_001254"
     CDS             complement(3414..4109)
                     /locus_tag="O2692_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HCZ4298889.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVQAKEKQERVQHLYDIK
                     DLHRYYSSESFEFSNISGKVENYNGSNVVRFNQENQNHQLFLLGKDKEKYKEGIEGKD
                     VFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFSINKEEV
                     SLKELDFKIRQHLVKNYGLYKGTTKYGKITINLKDGEKQEIDLGDKLQFERMGDVLNS
                     KDINKIEVTLKQI"
     gene            complement(4530..5225)
                     /locus_tag="O2692_001255"
     CDS             complement(4530..5225)
                     /locus_tag="O2692_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL6"
                     /protein_id="HCZ4298890.1"
                     /translation="MKLKTLAKATLVLGLLATGVITTESQTVKAAESTQGQHNYKSLK
                     YYYSKPSIELKNLDGLYRQKVTDKGVYVWKDRKDYFVGLLGKDIEKYPQGEHDKQDAF
                     LVIEEETVNGRQYSIGGLSKTNSKEFSKEVDVKVTRKIDESSEKSKDSKFKITKEEIS
                     LKELDFKLRKKLMEEEKLYGAVNNRKGKIVVKMEDDKFYTFELTKKLQPHRMGDTIDG
                     TKIKEINVELEYK"
     gene            complement(5671..6375)
                     /locus_tag="O2692_001256"
     CDS             complement(5671..6375)
                     /locus_tag="O2692_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HCZ4298891.1"
                     /translation="MKMTAIAKASLALGILATGTITSLHQTVNASEHKAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKNRKFTRVQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
     gene            complement(6739..7665)
                     /locus_tag="O2692_001257"
     CDS             complement(6739..7665)
                     /locus_tag="O2692_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HCZ4298892.1"
                     /translation="MKITTIAKTSLALGLLTTGVITTTTQAANATTPSSTKVEAPQST
                     PPSTKIEAPQSKPNATTPPSTKVEAPQQTANATTPPSTKVTTPPSTNTPQPMQSTKSD
                     TPQSPTTKQVPTEINPKFKDLRAYYTKPSLEFKNEIGIILKKWTTIRFMNVVPDYFIY
                     KIALVGKDDKKYGEGVHRNVDVFVVLEENNYNLEKYSVGGITKSNSKKVDHKAGVRIT
                     KEDNKGTISHDVSEFKITKEQISLKELDFKLRKQLIEKNNLYGNVGSGKIVIKMKNGG
                     KYTFELHKKLQENRMADVIDGTNIDNIEVNIK"
     gene            complement(7774..7911)
                     /locus_tag="O2692_001258"
     CDS             complement(7774..7911)
                     /locus_tag="O2692_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298893.1"
                     /translation="MFVKVAFLCLKSDETSNVPSVESHQNHFYLTNIMDFLIYLTMIQ
                     I"
     gene            complement(8030..9088)
                     /locus_tag="O2692_001259"
     CDS             complement(8030..9088)
                     /locus_tag="O2692_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HCZ4298894.1"
                     /translation="MKMRTIAKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKTLNISATPAPKQEQSQTTTESTTPKTKVTTPPSTNTPQPMQSTKSDTPQSPTIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFMLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVNHKVELSITKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSGTIVIKMKNGGKYTFELHKKL
                     QEHRMAGTNIDNIEVNIK"
     gene            complement(9379..10074)
                     /locus_tag="O2692_001260"
     CDS             complement(9379..10074)
                     /locus_tag="O2692_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HCZ4298895.1"
                     /translation="MKMKNIAKISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKL
                     KAYYNQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNIALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKEDHTGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVIKA"
     gene            complement(10360..11040)
                     /locus_tag="O2692_001261"
     CDS             complement(10360..11040)
                     /locus_tag="O2692_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HCZ4298896.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKAEVKQQSESELKH
                     YYNKPILERKNVTGFKYTDEGKHYLEVTVGQQHSRITLLGSDKDKFKDGENSNIDVFI
                     LREGDSRQATNYSIGGVTKSNSVQYIDYINTPILEIKKDNEDVLKDFYYISKEDISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMNDGTTHTIDLSQKLEKERMGESIDGTKI
                     NKILVEMK"
     gene            11512..12357
                     /locus_tag="O2692_001262"
     CDS             11512..12357
                     /locus_tag="O2692_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCZ4298897.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVPVRQLDYFNQESMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYAARLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
     gene            12376..12735
                     /locus_tag="O2692_001263"
     CDS             12376..12735
                     /locus_tag="O2692_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HCZ4298898.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGVLLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            13259..13411
                     /locus_tag="O2692_001264"
     CDS             13259..13411
                     /locus_tag="O2692_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298899.1"
                     /translation="MVPEEKGSITLSKEAAIIFAIAKFKPFKNRIKNNPQKTNPFLKL
                     HENKKS"
     gene            13672..13944
                     /locus_tag="O2692_001265"
     CDS             13672..13944
                     /locus_tag="O2692_001265"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCZ4298900.1"
                     /translation="MTKERRTFSSEFKLQMVRLYKNGKLKNEIIRKYDLKPSIISNSI
                     KQHQNTGPFNHQDNLKSDEKELIKLRKEVQHLKMEHDVLKQILKLI"
     gene            14343..15047
                     /locus_tag="O2692_001266"
     CDS             14343..15047
                     /locus_tag="O2692_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCZ4298901.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYS
                     IAIHSDVTSNKKRYYKSYSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALAQKYREIDIKLSTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DMNEIEKQLKIKFFSN"
     gene            15440..15976
                     /locus_tag="O2692_001267"
     CDS             15440..15976
                     /locus_tag="O2692_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298902.1"
                     /translation="MHNNNEFNKKLKDFIGSDKRMALVKGYVNEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNTIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
     gene            complement(16089..17630)
                     /gene="guaA"
                     /locus_tag="O2692_001268"
     CDS             complement(16089..17630)
                     /gene="guaA"
                     /locus_tag="O2692_001268"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCZ4298903.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCRGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(17655..19121)
                     /gene="guaB"
                     /locus_tag="O2692_001269"
     CDS             complement(17655..19121)
                     /gene="guaB"
                     /locus_tag="O2692_001269"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCZ4298904.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(19159..20427)
                     /locus_tag="O2692_001270"
     CDS             complement(19159..20427)
                     /locus_tag="O2692_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCZ4298905.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILLLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(20427..21005)
                     /gene="xpt"
                     /locus_tag="O2692_001271"
     CDS             complement(20427..21005)
                     /gene="xpt"
                     /locus_tag="O2692_001271"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCZ4298906.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(21236..21338)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            21518..21925
                     /locus_tag="O2692_001272"
     CDS             21518..21925
                     /locus_tag="O2692_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCZ4298907.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHQEVHENNSAYEEIDITHFAEASKGPKA"
     gene            22068..22730
                     /locus_tag="O2692_001273"
     CDS             22068..22730
                     /locus_tag="O2692_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298908.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDCIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            22848..23804
                     /locus_tag="O2692_001274"
     CDS             22848..23804
                     /locus_tag="O2692_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298909.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKALAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQSKNDNAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
BASE COUNT         7792 a         4228 c         3411 g         8557 t
ORIGIN      
        1 ataagtaatt tatctctttg tgtaatagat ttattataac atttggttat gttgcgatgt
       61 ggagaatttt gatgttggtg gtggaaattt tacctttatg gagattggga taggattcat
      121 gacacacaaa ccaaaacggc ttaacgacga tatcgattga tgcatccttt ttgtataagc
      181 tatgcgcacc aatttacatt tcaccgttaa gccgcttcaa ttacttttaa ttattcgtaa
      241 tgatttactt taagttaact tcaatatctt taatttgttt gctttctatg acttccccca
      301 tatatttaaa ttttaattta gatcttaaat caaggttata aaaactgcca tctttcaagc
      361 taattttgac cctaccatct tttgagattg ttttataaag tccgtatttt tcaattagat
      421 gctttctcaa cttataatcc agttctttta gtgttatttt ttctttttta atgtaataag
      481 ggtaaccttt cacaaaagca tcttcaccct tttcttttga aatatttaat attggtgcac
      541 tgacaactcc agatgtttta ttattctgta ttacaccacc aacagtataa aatatatcgt
      601 gcttatctct tttttcttga acaaagaaaa catctaaccc ctcataacta cgctgttgaa
      661 atttactttt atcatttcca ggcagtaaaa cttgaatctt aatacctcta aacttaaaac
      721 gtaagttgtt tttaccatgt ttcaaagcac taatattttt catttccata gtctttccag
      781 tgtagtatcg gtataatgct tccttgtcat gtttatttac tgacttttga ttttgttttg
      841 catgacctga atgaacttct gttgttaaag ttcctgttgt taaaattcct aaagctaatg
      901 tcgcttttgc taatgctgta aatttcatat ttatttgctc caatcttaat atattggatt
      961 gttttcatta cgtaatttga atcatacatt tttattataa cgtgcatcgt ttcatacgat
     1021 aattaacctc gatttaaata tacttgaatt attcttaatg aaaaacaaat atattttaat
     1081 gttcaggtta aatgactttc attatttcat ttagttgttt taccggatta tcgtttacta
     1141 caactgatga tgttactctg ttctgctctt tgaattacaa aaacaactta acaacagtaa
     1201 tgtatttaac acagttcctt atataaggac acaaatgcca attcacattt tgtcgttaag
     1261 ccgcttctta agctattatg ctattaactc gaactaattc aaattcactt caatattttt
     1321 aatttgctta ctatccatta catcaaacat acgttcaaaa cttaattttt cacttaaatc
     1381 aatttcatgc ttcttttcgt ctttcatatt gataacaatt ctacctttat cagacgttcc
     1441 tttatacaat ctatattttt cgattaagag ttttcttatt ttaaagtcca gttccttcaa
     1501 tgatacttct tccttttgaa taaaaaacaa ctcgtttatc gaaaagccat ttttagcatc
     1561 aactttttta acttgtagac ttggattact tacaaatcca aacactgatc tcacattttt
     1621 ctttgttata ccgccaacgc tatatatgcc accttttata tctattaatt caggtactgc
     1681 aaagacatca aggccatgtg ttttttcttt atatttattt ttatcgtcac caagtaaaaa
     1741 tactttaaaa gctttatttc gttgtttaaa gtttaaaacg ttagagccat agtaatcttc
     1801 gcttttaaca ctaatgtttg ttggttcaaa actttcttca gaatagtatt gatgtagcat
     1861 attgatataa tatttgcgtt gtgtctcatc aagttctact ttcgcgtgac cagtttgact
     1921 ttctgctgta aacacacctg tagttaatat tcctaatgct aatgttgctt tagctatcgt
     1981 tgttaatttc atattttttt gctccaatct taatgtattg gattgttatt attacgtaat
     2041 ttgaactatc tgtctttatt ataaggtgca tcaaaacaga ctgacattaa cgctcattta
     2101 aataaacttg aatcattatt aatgaaaatc aaacgtattt taacattcgt attaactgat
     2161 ttttaatagc aacataaata tttttttggt gaatcattgt tctttccgat tagttttaaa
     2221 tcttaagcta ttggttataa agtgtacaaa aaagcagctt aataacgtag tcaatgttcg
     2281 atatgttttt attataaaaa cacgtatcaa aatcattaaa catgggatta ttaaaccgct
     2341 tcttaagctt tcattctata tatatcattg attatttcaa attcacttcg atgtttttaa
     2401 tttgttcact attaatgaca tctgccatac gctcgaaatc taatttatca cttaaatcaa
     2461 tttcatactt attttcatct ttcatattaa taacaattct acctttatca gctgaccctt
     2521 catacagttt gtatttttta atcaacagtt ttcttatttt aaaatcaagt tccttcaatg
     2581 acacttcttc cttttgaata aagaaaaatt catcaataga aaaaccgtct ttatcgtcta
     2641 tttttttaac tagtaagttc ggcgttctta gagactcaaa tattgatttt acgtttttct
     2701 ttgttacacc actaacacta aatattcttc catctaaatc tactaattct ggtaccgcaa
     2761 agacatctaa accatgtgtt ttttctttgt atttattttc atcttttccc aataaaaaca
     2821 cttggaactt ttgattttgt tggttaaaaa gcaaaacgtt agagtcataa taaccttgac
     2881 tttggccact aacatttgtt aactcataac taggtcctga atagtattga tgtaacttgt
     2941 ttgtatcata taaacggttc attttttcat actttccttt tgcattaacc gattgatttt
     3001 ctgttatcat tacacttgtt gttaatattc ctaatataaa tatcgcttta gctatcactg
     3061 taaatttcat attcatgtgc tccaatctta atatattgga ttgcttttat tacgtaattt
     3121 gaataataca cctttattat aaagggcatt gagacagact gacattaaca ctcgtttaat
     3181 taaacttaaa tcattgttaa tgaagagcta attaatattc aatgggcatc actttcaaga
     3241 gcaatgtaaa tttttagtat gctcactcat aacctgcgtt ataaaacgta aaaaaaagcc
     3301 taacgacaag gttgttttaa atacgctcct taatgtaagg tgtacgtatc tattcaacat
     3361 ttcatcgtta agctgcttca aatttattac tttagagtca ttgattactt actttaaatt
     3421 tgtttcaaag tcacttcaat cttattaata tccttactat tcaacacatc acccatgcgc
     3481 tcgaattgca atttatcacc taaatcaatt tcttgctttt ctccatcttt caaattgata
     3541 gtgatcttac cgtatttagt cgtaccttta tataaaccat aatttttaac taaatgttgt
     3601 ctaattttga aatcaagttc tttcagtgaa acttcttctt tattaattga aaatgagtca
     3661 attgatgcat ctaaatttcc gccatacact ttattaacaa ataaatgtgt attagtttca
     3721 gaagatttgt tatttttctt agtcacacca ccaacagtag ataatctacc gtttggatca
     3781 attaattctt ttaccacaaa gacatctttg ccttcaatgc cttctttata tttctcttta
     3841 tctttaccta ataagaataa ttggtgattt tgattttctt ggttaaagcg tacaacgtta
     3901 gaaccgttat aattttcaac cttaccacta atattactga attcaaaact ttctgatgag
     3961 tagtatcgat gtaagtcttt aatatcatat aaatgttgta ctctctcttg cttttctttt
     4021 gcttgaactg cttggccttc tgatgtaatc acaccagtag ttaataagcc taatgccaat
     4081 gttgctttag ctaacgtttt taatttcaca gtactattct cccaatctat ttataaattt
     4141 gtcttaatat atttttatat gattaattca attacgtaat acgaacaatc tactatcatt
     4201 atataagtga gatactgcgc tatgaattaa cctcttttta actatttttg aaccaacgtt
     4261 aagttagtta atgaaatcct aacagtatta attatttttt ataacacaat actaattttt
     4321 taactattca cactttccct caatttaatt atgttttcca cattgtttca tgtcacgaaa
     4381 aggacaacgc gcgactataa gtatcaaccg tttttccaaa aacttgatca taaacccgct
     4441 ccttttttca tcataacaaa ataaaaaaga agctaagcaa tatgttatcg cttaacttcc
     4501 ctacaattac tagtctgctt gtccaaagat tatttatatt ctagctcaac attaatttct
     4561 ttgattttgg taccatctat cgtgtcaccc atgcgatgcg gttgtagttt ttttgtaagt
     4621 tcgaaagtat aaaacttatc atcttccatt ttaactacaa ttttaccttt tctattatta
     4681 acagcaccat ataatttttc ttcttccatc aatttttttc ttaatttaaa gtccaactct
     4741 tttaacgaga tttcttcttt agtaatttta aatttactat ctttagactt ttccgatgat
     4801 tcatcaattt ttcttgttac tttaacatcg acttctttac taaattcttt actatttgtc
     4861 ttacttaaac caccaattga atattgtctt ccattaacag tttcctcctc gatgactaaa
     4921 aatgcatctt gcttatcatg ctcaccttga gggtattttt caatatcttt accaagcaag
     4981 ccaacaaaat aatcttttcg atccttccaa acatatactc ctttatctgt cactttctgt
     5041 ctatacaaac catcaagatt ttttaactct atacttggct tgctatagta gtattttaac
     5101 gatttataat tgtgttgacc ttgagttgat tctgccgctt ttactgtttg actttctgtt
     5161 gttattacac cagtagctaa caatcccaat actaatgttg ctttagctaa cgtttttaat
     5221 ttcatagtac tattctccca atctatttat aaattttgtc ttaatatatt tttatatgat
     5281 tatttcaatt acgtaatacg aacaatctac tatcattata taagcgtaat actaagctat
     5341 gaattaacct ctttttaact atttttgaac caatgttaag ctaattaatg gaatcctaac
     5401 agcgttaatc tattttttaa acttaacgca gcttttttaa ctattcacac tttccctcaa
     5461 tttaactatg ttttccacat tgtttcatgt cacgaaaagg acaacgcgcg actataagta
     5521 tcaactattt ccacaagttt tattggtgtt tttattattc atcgatacgc ttcattttca
     5581 tctctccaac acaaaaaaga agctaagcaa cttatgttgc ctaactcctc tatactatcc
     5641 atattttact attatccata tttcattgaa ttatctaatg ttggcttcta ttttttcaat
     5701 atttctaccg tcaatgacgt cactcatgcg atttgtttgt aattttttat taagttcaaa
     5761 cgtataatag ccgccatctt tcattatcac ttttatctta ctatctttag gaaacttttt
     5821 atacagatca aaattttgaa ttaaatactg tctcaattta aagtcgagtt ctttaagtga
     5881 aatctcttct ttataaatgt agtgtactct accgtacgta gcaataccgt caccttcatc
     5941 tctcttgatt tgaaatcttg gtgcgtttat ataatcataa taagcgtctt gatttttctt
     6001 agtgacacca ccatatgaaa acactgtgcc attacggttt tccgcttctt taacaacaaa
     6061 tatgtctaat cccggatttt tacgtgcttt aaatctttca atatctttac caaatatctg
     6121 tactcttgtg aattttctat ttttatcaaa gataaggtaa tgcttgccac ctttgctata
     6181 acgataacca gtaacatttt taagttcctt acttgcgcca ctatagtaat ctcttaagtc
     6241 aaagatatct tttgtcacat tttcatattt tgctttatgt tcactcgcat ttacagtttg
     6301 atgcaatgac gttattgttc ctgttgctaa aatacctaat gctaaacttg ctttcgcaat
     6361 tgctgtcatt ttcatagttg tatgctccat tcgtaattat tagatttgtt cgcttacgtc
     6421 tattgaatca tacagcttta ttatagttag cgtatttgac ctttcacatt aaaccatgtt
     6481 taataatcat tgaatcatta ttaagtaaat taaggaatct ataatgttcg ttaaataaaa
     6541 ctgatcccgt tgtgcttcac acccgataga tagggattta cagataaatt caggtctctt
     6601 ccacgtcata tttggaccca tcgaaaattc gggttctcaa atcatcgaac ataacaaaag
     6661 aagctaagca acatgtaggc cgttgtcact taacttcttg tttttccgat gacagcttct
     6721 atttagagaa tgtcatgatt attttatatt cacttcaatg ttatcaatat tagtgccatc
     6781 tatgacatct gccatgcgat tttcttgtaa ttttttgtgc aattcaaacg tgtactttcc
     6841 accgtttttc attttaataa caattttacc tgaaccaacg ttaccgtaca gattattttt
     6901 ttcaataagt tgttttctca atttaaaatc aagttctttc aaggaaatct gttctttagt
     6961 aatcttgaat tctgaaacat catgagagat tgtaccttta ttatcttcct tagtaattct
     7021 tactcctgct ttgtgatcaa cttttttact attactcttt gtgataccac cgacagaata
     7081 tttttccaga ttgtaattat tttcttctaa aacgacaaat acatcgacat tcctatgtac
     7141 tccttcacca tattttttat catctttacc aactaaagca attttatata tgaaataatc
     7201 tgggacaaca ttcataaatc ttattgtcgt ccattttttt aaaataatac caatctcatt
     7261 tttaaattct aaacttggtt tcgtataata cgctcttaaa tctttaaatt taggatttat
     7321 ttctgttggt acttgttttg tggttggcga ttgtggtgtg tctgatttag tagattgcat
     7381 tggttgtggc gtgtttgttg atggaggtgt tgtcacttta gttgaaggcg gtgttgtcgc
     7441 atttgctgtt tgttgcggtg cttctacttt agttgagggc ggtgttgtcg cgtttggttt
     7501 tgattgcggt gcttctattt tagttgaggg cggtgttgat tgtggtgctt ccactttagt
     7561 ggaagatggt gttgtcgcgt ttgctgcttg cgttgtcgtt gtgattacac ctgttgttaa
     7621 aaggcctagt gctaaacttg ttttagcaat cgttgttatt ttcatagttg tatgctccat
     7681 tcgtaattat tagatttgtt cgattacatt cattgaatca tacagcttta ttatagatgg
     7741 cgtattgctc cattcacatt aaaccttgtt taactatatt tgaatcatcg ttaagtaaat
     7801 taagaaatcc ataatgttcg ttaaataaaa atgattttga tgtgattcaa cacttggcac
     7861 atttgaagtt tcgtcacttt taagacatag aaatgccact tttacaaaca aatgaatatt
     7921 cgtcttttta catcattacg cataataaaa gaagctaagc aacatgtaaa ccgttgtcac
     7981 ttaacttctt gtttttccga tgacagcttc tatttagaga atgtcatgat tattttatat
     8041 tcacttcaat gttatcaata ttagtgcctg ccatacgatg ctcttgcagt tttttgtgta
     8101 attcaaacgt atatttccca ccgtttttca ttttaataac gattgttcct gaacccatgt
     8161 taccgtaaag attatgtttt tcaataagtt gttttctcaa tttaaaatca agctctttca
     8221 aggaaatctc ttccttagta atcatgtatt ctgaaacatc gcgtgaaatc ataccttgat
     8281 tatctttttt agtaatgctt aattctactt tgtgattaac ttttttacta ttagtcttcg
     8341 tgatgccacc gacagaatat tttttcaatt gatatttatt gtcttctaaa acgataaata
     8401 catcgatatt atcgtaaggt ccatctttat attttttctc atcttttcca actaaagcta
     8461 ttttatagat gaacctattt ggaataacat tcataaacct aaccgtcgtc catggtttga
     8521 gcataaatcc aaactgcttt tcaaattcaa aactcggttt tgtataatac gctcttaaat
     8581 cttcatattt aggagtcata tctgtttgtg cttgttttat ggttggagat tgtggtgtgt
     8641 ctgatttagt agattgcatt ggttgtggcg tgtttgttga tggaggtgtt gtcactttag
     8701 ttttcggcgt tgtggattcg gttgtcgttt gtgattgttc ttgtttaggc gctggcgttg
     8761 ctgatatatt aagcgttttc tgctcttctt gtttaggttg tgatattttt tctattttgg
     8821 aagctgaggt tttttcctca ttagtatttg gtgccttttc gagtttaggc gtgcgttctt
     8881 gtcttgtgtt agctgcttgt gttgtcgctg aatttgcacc tgctgttatg tttatcattg
     8941 ctaatcgctc tgctttaagc gttggtactt tgtcaacttt agttgattgt attttttctg
     9001 ctttgaccga ttgcgtcgtt actgtaattg cgcctgttgt taaaagccct agtgctaaac
     9061 tggttttagc aattgttctc attttcataa ttgtatgctc caatctatat tatattcgat
     9121 tgtcttttta cgtaatttga atcatacaac atcattatag atggcgttct aagataatca
     9181 cattaaaccc cttttaacaa ttattgaagt attattaagt aatttaagca aaaaataatg
     9241 agtgagtatg agattaatat agcgtttcta tgtgcctttg aaataatttt taagcattaa
     9301 aaagaagtta agcaacgttt gatcgtcact taacctctct atttcaattt caacttattt
     9361 cgtcatcaag tatatgtgtt atgcttttat aactttgatt tcaattctat caatatctgt
     9421 gacattgata acatcggaca tacggtcttc ttgtaacttt ttatccaatt caaatgtata
     9481 ctttccatag tatttctttt tgactgtaat ttttcctgta ctcatttcac cgtaaagacc
     9541 ataattatca ataaggtatt ttcttaattt aaaatcaatc tctttcaatg acatcgcttc
     9601 tttatctatt ttaaatggga aaaagtcata atcatattca ccagtatgat cttctttaat
     9661 aactcttgct tctgctatta ggtcgacagc tttatcgttt gcactcgtga tacccccaat
     9721 agagtacttt gcaccttcaa atctcttatc ctcattaacg taaaatatat taagattacg
     9781 atgtacaccc gtatgataat gttgcttatc tttgccaatt aaagcaatat tattaacaga
     9841 attaccatct atgatattca taaatttaat acttggttga atgaaactga tataacctgt
     9901 cacattttta tattcaatac taggttgatt ataataagct tttaattttt tgctattttc
     9961 acttattaca ataggtttct tttcggcatg aactggtttt tccgttgtag tgtttacacc
    10021 tgttgctaat attcctaata acaaacttat ttttgcaata tttttcattt tcatagttgt
    10081 atgctccaat ctattataat tagattgttt tattacgtaa tttgaatcat acacccatat
    10141 tataggagct gtattcggat attcacatta acctgttttt aactattcat aaaatatgat
    10201 taagctattt aagcaaaaga taatgcgtgg tgcgactgtt tgtgtgagtt ttgtaacttt
    10261 tcccctcaaa aaaagaagct aagcaacagt acgtcgctta acttccatat atttccataa
    10321 caagcactat tcaacatagc gctttgttgt tagaaagtat tatttcattt ctactagaat
    10381 tttattaatc ttagtgccgt cgattgactc acccatacgt tctttttcaa gtttttgact
    10441 taaatcgatt gtatgtgttg tgccatcatt cattgtaatt gtaatttgac cttgtttaag
    10501 accatttgaa tacaagccgt gttgtttaat cgcacgttct cttaatctat aatcaagttc
    10561 ttttaatgag atgtcttctt ttgaaatgta gtaaaaatct ttaagtacat cttcattatc
    10621 tttcttgatt tctaaaattg gcgtattgat ataatcaata tactgcacac tatttgattt
    10681 tgtaacgcca ccaattgagt aatttgttgc ttgtctactg tcaccttctc taaggataaa
    10741 cacatctata tttgagtttt ctccgtcttt aaatttatct ttatcagatc caagtaaagt
    10801 gattcgagaa tgctgttgcc ctactgtgac ttctaaatag tgtttaccct catcagtata
    10861 tttaaatcca gtcacatttt tacgctctaa aattggttta ttataatagt gttttaactc
    10921 tgattcactt tgttgtttaa cttctgcttt cgcttgtact gattgtacat tcgatgtaat
    10981 tacacctgtt gctaacattc ccaatgctaa acttgctttt gctatcgctt taaatttcat
    11041 aatttttagc cccaatctat ttaaatttga tttttcaatt acgtaatatg aacaatctac
    11101 ctacattata ttgaggcgct atcccctttc aaattaaacc tcatttaact aaacttgaat
    11161 cattgttaag gtaattaata aaatgctaac tgttattaat caattcttat ccgtcgagac
    11221 tcttttttat acaaatcaca ctttatacca tttttatttt aaaaactgta aaactttcag
    11281 acacaaaaca gacatcatat tgtcgtagta cctgattaaa attttcaatg atattagtga
    11341 tacacatcaa ctaaaacgcg tcattacggc attttcaaca ttaataatat ttacgtattt
    11401 actatacacg tcgacaataa atacagatta gaatttttta taaaatcaaa tgttttgaaa
    11461 accatcaaat aagtattatg ttatgtatat aaagattagg agatgaagtg aatgaatatt
    11521 atgttaacag gtgctacagg tcatttaggc acacatatta caaatcaagc cattgcaaat
    11581 cacatagatc attttcacat tggtgttaga aatgttgaga aagttccaga agattggcgc
    11641 ggaaaagttc ctgttcgaca attagattat tttaatcaag aaagcatggt agaagcattt
    11701 aaaggaatgg ataccgttgt gtttattcca agtattatcc atccatcatt caaacgtatt
    11761 cctgaagtgg aaaatttagt atatgcggca aagcagagcg gcgttgctca tatcattttc
    11821 ataggttatt acgcagatca gcataataat ccattccata tgagtcctta ttttggttat
    11881 gcagcacgtc tattggcaac aagtggcatt gactatacgt atgtaagaat ggcaatgtac
    11941 atggatccac ttaaaccata tttaccagaa ttgatgaata tgcataaact gatttatcca
    12001 gctggcgatg gtcgtattaa ttatattact agaaatgata ttgctagagg tgtcattgct
    12061 attattaaaa atccagatac ttggggcaaa cgctacttat tatcaggcta cagttatgat
    12121 atgaaagaac ttgctgcaat tttatctgag gcatcaggca cagaaattaa atatgagccc
    12181 gtttcattag agacatttgc agaaatgtat gatgaaccta aaggctttgg tgcattattg
    12241 gcatcaatgt acgacgcagg agcaagagga ctattagacc aagaatccaa tgatttccaa
    12301 caattagtca atgatcaacc acagacactg caatcatttt tacaagaaaa tatttaataa
    12361 taaaggagcg ttatagtgaa tatcatctca acaattttaa tcatatttgt ggcattagag
    12421 tttttctata ttatgtacct tgaaacgatt gctacaactt ccaaaaagac tagcgagaca
    12481 tttaatataa gcgtcgataa attgaaagac aaaaatatta acctactttt gaagaaccaa
    12541 ggcgtatata acggtttaat cggagttttg ctaatatacg gtttgtttat cagcagtaat
    12601 ccaaaagaaa tatgcgcagc tattttagtg tatatcattg gcgttgctat ttatggtggc
    12661 ctttcaagca atattagtat ctttttcaaa caaggcacat tgccagtatt ggcactcata
    12721 tcaatgcttt ggtaagtatt ggtgtttggg ggggtggaga tgtagtcgga ggtttggagg
    12781 atttgagcga attgtgtgtg gactttagac tcagagtatt tcatcctaat tatttcaagc
    12841 agaggtgaca gtagcgttgc ctctgtttcc ttatagaaaa attatttaat gaagaaaacc
    12901 catatctgat ttaattttca gctgataaat actccatata ttagaatggc tactttatct
    12961 attgcatcaa tcctttaaaa caaaaaaccc atgatttcga aattcccgta tgatggggtt
    13021 cctactctca tggatcagtt aaataaatat tatcactatc agtttattat ttcaatatta
    13081 ttaacaatat atgtagtcgt aaaaggaaag aggacatgag aacttcggtg ttgattggca
    13141 ttacataacg cttccaaaca tattatttgg taacaataag aaactattta cacaatatat
    13201 tttgtatagt aaaattattt tataatattt aaatccaatt gcacaaggag tgattatcat
    13261 ggtaccagaa gaaaaaggtt ctattacttt gtcaaaagaa gcagctatca tatttgcaat
    13321 cgcaaaattc aaaccattta agaacagaat taaaaataac ccacaaaaaa caaatccatt
    13381 tcttaaatta catgaaaaca aaaaatctta atcactttat ttatagcatt tctaatctca
    13441 gaaatgctat aaaggacaac gccctctttc ataactatat aagtcaagaa accaagacat
    13501 ttcaaacatc tttaacccct tcaatagcac attccctaaa acataaagca ataacaatga
    13561 acgatcaaaa ttccttacgt acttatgatg tgtcagtgaa tcgtctacac ttaattgaac
    13621 aaattctatg agaatagata ttgttaaatt aagaaagtag tcaattttat tatgacaaaa
    13681 gaaagaagaa catttagttc agagtttaag ttacaaatgg ttagattata taaaaatggt
    13741 aagcttaaga atgaaattat acgaaagtat gatttaaaac cctcaattat ctcaaattcg
    13801 ataaaacaac accaaaatac tggacccttc aatcatcaag ataatttaaa aagtgatgaa
    13861 aaagagttaa taaaattacg caaagaagtt caacatttaa aaatggaaca tgatgtttta
    13921 aagcaaattt taaagttgat ttagaaactg aactttttaa agatacatta cttgaagatt
    13981 ctaatttatc tgattatata aagaaccaac ttccaacgat atggagagac taagaaatag
    14041 tttaaattta tttaatttca ataaaaactc ataataatat ttaagtaagt tagattgctt
    14101 ttagcattag gaattcgctt ataattagat gttcaatata gactttttta cacatacatg
    14161 aactataaaa agcgaataaa tagcaggtca ttgcgaaatt tgctaaatac aaaattgatg
    14221 agttccacct aataaaaatt gttggtttta tagtaacatc tattttcgta ccattcatgt
    14281 taatttcagt gctaataatg ctgatataaa gaagaataaa ttgattagga gaacactatt
    14341 taatgtctaa aaatatcaat caagcaaata gtaaattaaa tacgtcaaac aaaaaactaa
    14401 aacaagctta ttcaaaatcc gacagtaaaa acatgaaagt tatatacatg cctcattggc
    14461 tagagtttta ttcaattgct atacatagcg atgttacaag caataaaaaa agatattata
    14521 aatcatatag tagaggaaca gtggtatatg taaaactagg gagtaatatt ggaagtgagt
    14581 tttctggtaa tcatttttgt gtaatattag ataacaaaga caataaagga aaagaaacag
    14641 ttacaatagt tccactttct tccaaaggta ataaaaatta tttgaagtta aacgaatcgg
    14701 tgctaaatct aacagcaaca gatttaaaaa aacagattat cgatatcagt agtaaagtac
    14761 aagcattagc ccaaaaatat agagaaattg acattaaact tagcacagaa gataaaaagc
    14821 ttttgtctaa cttaaatcaa aaagctaatg aactgtatag agtgataggt gtttattcca
    14881 agcacaaagg gaaagataca tacgttaata tatcagcaat tacaacaata agcaaaagac
    14941 gtataagcaa aataaatgat tctgatccta ctggaacgat aataatttca gaagatgaca
    15001 tgaatgaaat cgaaaagcaa ttaaagataa agttcttttc taattgaatt gtaatattac
    15061 atctgatata attgatttac aaattcccgg taaccattcc ggcttcattc tcttgaagaa
    15121 tgacatattc tcagcgtttt agctgaaggt cagatgatac gtcatctggc ctcttttttt
    15181 atataaatgc cctttaagtg aaatatacaa caatctgcaa tcaatattag acatcacaaa
    15241 acacacaata aattacacaa tgtgttataa gccacatgtc actattatcg cagctagctg
    15301 cgtcactcaa aacgaatacg gaacgcgttg gttatttcaa tcataatatt actctgcaaa
    15361 tacacagccc tgtaaccgag taaatggata gagatttgaa caaatgaaaa caatcaacta
    15421 atggaaagga taaaatatta tgcacaacaa taatgaattc aacaaaaaac tgaaagattt
    15481 tataggtagc gataaacgga tggctttagt aaagggttat gttaacgagt ataaattaac
    15541 aactgtttta agagctttaa ataattccga ttacacaaaa gggacgatac acactcgttc
    15601 aataggcaac ctaaaacaaa tagtagataa tcgaattatt cctaaaaata taactcaaaa
    15661 cacaatcttc aaattgagta atttaacctt agaggttaac ctctatgaaa gaaacacaat
    15721 ttatcacgga gactttgcta tttactatcc tgtagaatct gctctaataa tagaaacaga
    15781 tactcctagg ctaattaatc atataaataa caacccagtc caaaaaatat ttataataac
    15841 aacaaatgac tggagcttta atacacttga attagaaaaa atagtagacg aaactataat
    15901 ttatgattta aaacaagaag atcctaaaca atatgaaatt ttatataaaa ataaacacgg
    15961 aaaattgcct tattgatgaa ttaaaacgca aacttagaac accttacccc ttttccgtcc
    16021 ttattgtaat tttccaacta ctcaaattaa aaaccacccg tgcaaacagg tggttttatt
    16081 atatataatt attcccactc aattgtgctt ggtggttttg atgtaatgtc atagactacg
    16141 cggttgacgt gatctacttc gtttacgata cgactagaaa tcttttgtaa gacttcccaa
    16201 tcgatgcgtg cgaagtcact tgtcataccg tcgatagatg ttactgcacg aatacctact
    16261 gtgtgatcat acgtacggta gtctcccata acacctactg attgaatgtt tggtaacact
    16321 gtgaagtatt gccaaatttc tctttcaaga ccttcttctc taatcacttg gcgtaaaatc
    16381 gcgtctgatt ctctaacgat ttctagttta tcttcagtaa tttctccaag tacacgaata
    16441 ccaagaccag gtcctgggaa tggttgtctc catactaaat gttctggaat acctaactca
    16501 atacctaatt tacgtacttc atctttaaac aatgtattga ttggttcgat taattcgaat
    16561 tccatgtctt ctggtaatcc accaacattg tggtgtgatt tgattgtttg tgctgtttta
    16621 gtacctgatt cgatgacgtc tgtatatagt gttccttgcg caaggaagtc tacacctttc
    16681 agttttgatg cttcatcatc aaatacgtat acaaattcat taccaatgat tttacgtttt
    16741 tgttcaggat ctgaaacacc ttttaattta ttcataaagc gatctttcgc attaacacga
    16801 ataatattca tgttgaaacc ttcaccgaat tgctccataa ccatgtcgcc ttcaccttta
    16861 cgaagtaagc catggtctac aaagatacat gttagttgat cacctattgc tttatgcaat
    16921 agtacagcta caacagatga atctacgccg ccactcatcg cacataatac acgacggtct
    16981 cctacgcgtt gacgaatctt ttcaatttcg atttcgataa agttttccat tgtccattga
    17041 cctctacaat cacaaacacg acggacaaaa ttatttaata aatcattacc atattctgta
    17101 tgacgtactt ctggatggaa ttgaacacca taaatgcgac gtttcttatc ttcgattgct
    17161 gcatagtctg tgcttgggct atcagcgata acttcaaagc cttctggaat ttcaataact
    17221 ttatcagaat gactcatcca aacagtttgt tctgctggta agccagcgaa taactcatct
    17281 gacttcgcat taatgattgc tttaccgtat tcacgttcat tggcacgttc aactttacca
    17341 cctaataatt tagtagttaa ttgcatgccg taacaaatac caagtactgg aattcctaaa
    17401 ttatatattt ccggatcaat tgtaaatgaa ccttcttcat aaactgaatt tggaccacct
    17461 gataagataa taccttttgg attcattttc ttaatttctt caattgaaat ttcatgatcg
    17521 tgtaattcac tataaacgcc catttcacga attcggcgtg taattaattg gttgtattgg
    17581 ctaccaaagt ctaagacaag gattaactct tgttcttttg ccatttccat atttgtcgtt
    17641 ctcctttatc ttaattagaa tgagtagttc ggtgattctt tcgtaatttg aatattatgt
    17701 ggatggcttt ctgctaaacc agcaggaccc atacgtgtaa attgtgcttc ttcgcgtaat
    17761 tctcttaaat cgtgtgaacc agtataaccc ataccagcac gcacaccgcc cattaattgg
    17821 taaattgtat cttgtaacgc acctttataa gccgtacgtc cttcgatacc ttcaggaaca
    17881 aatttcttag gcgctttgtc ctcttggaag taacggtcgt ttgaaccttt ttccatcgca
    17941 cctaaagagc ccataccacg gtatacttta tattgtctac cttggaaaat ttctgttgcg
    18001 cctgggcttt cttcagtacc tgctaataag ctacctaaca taaccgcatg tccaccagca
    18061 gctaatgctt taatgatatc tcctgagaat ttaataccac catcagcaat gatagcttta
    18121 ccatgtttgc gtgcttcagt tgcacaatca taaattgctg taatttgtgg tacaccaaca
    18181 cctgctacaa cacgcgtcgt acaaattgaa cctgggccaa taccaacttt aacaatatct
    18241 gcacccgctt caaataaatc ttttgttgct tctgcagttg ctacgttacc tgctactaat
    18301 gtgatttctg ggtaagtctt cttaatatgt ttcacttgat cgataacacc tttagagtga
    18361 ccatgtgctg tatcgataac taagacatcc acacctgctt cgactaattt ttgagcacga
    18421 atatcagtat cttttgaaat accaattgct gcggctacaa gtagacgacc atgttcatct
    18481 tttgctgcat tagggaattc gataactttt tcaatatctt taatagtaat aagaccttct
    18541 agacgtccgt ctttaactaa tggtaacttt tcaatcttat gtttttggag aattttttct
    18601 gcttcttcaa gtgttgtatt cactggagct gtaattaaat tttcttgcgt cattacatct
    18661 acaattttaa tcgagaagtc ttcaataaaa cgtaagtcac ggtttgttaa aatacctact
    18721 aagttgcgat cttctttatt atcaacaatt ggtacacctg aaatacggta tttacccatt
    18781 aatgcttctg cttcataaac gctttcttct ggcgttaaga aaaatgggtt tgaaatgaca
    18841 ccattttctg agcgttttac tttttgaact tcgtccgctt gttcttcaac gcccatattt
    18901 ttatgaataa cacctaaacc accttgacga gccatagcaa tcgccatttt agattcagtt
    18961 acagtatcca taccagcaga aataactgga atatttaatt taactttgtc tgataattgt
    19021 acgcttaaat caacgtcttt cggtaaaata tcagattgtg ctggaattaa taacacatca
    19081 tcaaacgtta atgattcttt tgcaaattta ctttcccaca ttaaaaacag cctccatttt
    19141 tcaaattaat tagttatatt atttcacatt ttcttccgtt tgtttatact ttattccatt
    19201 aaaaaagaaa ttaagaataa ttgcagagat tgcgcctaaa acaattccgt tttgagtcaa
    19261 ccatgcaaat tgttcaccta aacctttaaa tgcttgtggt acagcgctta taccagtacc
    19321 taatcctact gatacagcga taattaataa attgttttga tttttaaaat cgatatgtcc
    19381 taatatacta acaccatatg ccattaccat gccaaacata gctatcatcg caccgcctaa
    19441 cacaggtagc ggtatgatat ttgctaatgc gccaagctta ggtatacaac cacatataag
    19501 taataacacg accatgccgt atataacatt gtttttctta gcgccggata aagaaacaag
    19561 tcctacattt tgcgaatagg ctgtatacgg aaatgaattg aatatagaac ctaacactat
    19621 cgctagacct tccgcagtat aacctttacg aaaatctttt ctttctaact tcttaccggt
    19681 aatttcactt aacgcatgat agacacctgt cgactcaatt aaactaacga tagctacaat
    19741 aaagaacact aacgtcgatg tcacatcaaa gctaaatcca gagaatctaa acggcactgg
    19801 gatgcctaac caaccggcat gattgacttg attaatatcg accatcccaa gtaagccagc
    19861 acctatcgtt cctaaaacga gtccaattaa tatggcaata ctcttaataa atccagttgt
    19921 gaatctttgt aaaagaagaa taatgattaa tgtcattaaa cctaacaaaa tgttcttaac
    19981 atctccatag tcctttgcac cttgacctcc agctaagtaa ttcattgcta ctggcattaa
    20041 attgatacca atgatagtaa caacactacc cgttactact ggtgggaaga attttacaag
    20101 atgtgaaaag aaaggcgcga tgataataac taatatccct gataaaaata gcgaaccata
    20161 aagtacatct attcctttcg tttgaccaat taaaatcatg ggcgcaacag ccgtgaatgt
    20221 acatccaaga acgattggta atcctgttcc tgttacttta ttggcttgta aaaatgtggc
    20281 aaccccacac ataaatatat ctactgtaac taagtaagcg atttgttcag gtgtaaactt
    20341 caaacttgta ccaacaatga ttggaactaa gatagcacct gcgtacatag ctaaaagatg
    20401 ttgaacactt aggattaaat ttttcattat tcttctccca ccaatgtcac tttgtttcct
    20461 tctagtgaag caaccttgca gagagaagaa actgttaaac ctgcttcttc taaacgttga
    20521 tgcccatttt ggaaactctt ttcaacaaca ataccaatac cagctgtctt agcattcgct
    20581 tgctgtgcga tatcgtataa tcctaatgaa gcatcaccat ttgctaaaaa gtcatcgatg
    20641 ataagtacag tatcttcttc tgataaaaac tcttttgaaa caatgaccgt acttgtttta
    20701 tttttagtaa atgaatgaat agatgtttca taataaccat ccgtcaaagt gctaggtttt
    20761 gcttttttcg caaataaaca tggcacatca aaatgcagtg cagccatgat tgcaggtgcg
    20821 ataccggaag cttcaatggt taagatttta gtaatccctt tatctttaaa ttgctcgtaa
    20881 aaagtgcgac caacttcatt cattaacttt gcatcaattt gatgatttaa aaatccatcg
    20941 acttttaaaa tcttctcatc aatgacaacg ccgtcttcct ttactttttg tcctagtaac
    21001 tccactctta aaacctcctc agatttgtgt gaaacatttt gctcttaaat tggtgctaga
    21061 tacaaaaaaa tccccaaact aaataatagt ttcagggttt atgagtgaac gaacatgcat
    21121 aacgaatttg tcatgcaatc aatgtaaaag aagtttcatc aaataaactg tgaccatcat
    21181 ataaaatgat ataaatcacc caccatggtt acaatttaat ggctgaagct actcctagta
    21241 ttgtgttgtt actcatagtc atgtcgttca aggcaacatg gtagaaactt ctaaagccat
    21301 attctttaga ttatatgagt ttatgtaaat tatttaacga taatagcaaa ttttcggcat
    21361 tttttcaata actgcttagg taatctttta atagttttat aaatgtacag agacgtttaa
    21421 tgcgaatgaa gacttataac ttaataatgt aagggtttta ctttataata agaagggtaa
    21481 aagattaaat aaacaaattt ttaaaggggc gacaactatg gcagatatta ctgtagtaaa
    21541 tgacactggc gaattataca acgtaatcaa tcaaaagaaa tctgaaggtt atttagaatc
    21601 agagctaaca attattagta aaagtaaatt acacctaaat gacttacacg actctgaaat
    21661 ctcccttatc tcaacaagtg gaacatttag tgatagaatg actaaactgt taacaggaga
    21721 agatggtgaa catgcagttt tatcacgcta caacttagcg cctgatgaat tagaaaaata
    21781 taaacaactt attttagacg ataaaatgtt agttgtagcg gttcgtgaca aatcatcaca
    21841 tcaagaagtg catgaaaata attcagctta tgaagagatt gatattacac attttgctga
    21901 agcatctaaa ggtcccaaag cttagttatc acactgaatt caaaatgaga ttactgtact
    21961 gtctaattta agatagtaca gttttttata ccatttaaaa gtttatgaac agtcttttac
    22021 agcaggcgat tatgctataa tattatttaa attggggagg tgttcttatg gaatttaaaa
    22081 ccattcaatc tgtcaaagat ccacattaca acgaagcact taaactttac gatactaagt
    22141 taaatttatc tttaactgaa gataaacgta tcatcaaacg ctcattagaa aacaacaaaa
    22201 ccgaaaacga ttatgtcttt atcgtaggct tagaaaacga tgttgttgtc agtctagcaa
    22261 ctgcacatta tgaagccaca accaattcag catttttaat ttatttattc gcaaaagacc
    22321 tacccaacca tgatgagtta atgacgcaaa cattacatca aattgaacat gaactaaact
    22381 tattatcaaa ccttgttcat tctagaagta ttaacttttt aatgatggaa gtccctaaag
    22441 aacctgcaga tttaacacaa gataacggcc tcattattga acaccgtaga cagtttttat
    22501 acgaacatca atttgaaaga caaactgaaa ttgattgcat tcatccaact aatgatggtc
    22561 acactaaaaa agtggactta tttttaaaga aaaatattaa actgacaaag gatatctacg
    22621 gtacaagtgt taaatcaaat tatattttga agtatgtatt tgccaatggt atcgcacgtg
    22681 aaattattta tgctttatta gaagaaatgg atttacgtaa accattttaa acaaacttga
    22741 aatcactaac aataacgttt aaactatgta tgaagcaaga ctgacgtttc actgcccgta
    22801 gacaaacgtc agtatacata aagtatatgc agaaaggact ttaaagtatg ttaactaaag
    22861 aatttgcaca acgcgtagaa ctaagtgaga agcaagtacg taaaattgtt caacatttag
    22921 aagaacgtgg ttaccaacta agcaagactg aatatcgtgg tcgtgaagca actgatttca
    22981 aagaagaaga tatcgagctt ttcaaagaca ttgccgacaa agtaaaacaa acaaatagtt
    23041 atgatctagc gtttgatgaa ttagaaaaag aaaaagactt cctgcaagtc attgtcaaaa
    23101 acgatgacaa aaacttacct actaatcaaa atgtcgctca actagtagaa gatttacgcc
    23161 tagaaatcca gaaaatgcgc gaagaacgtc acctacttgg tcaaatgatg aatcaagtac
    23221 atcagcaaca acaagaatta aaagaacttc aaaatcaact tacatctaaa atcgattcaa
    23281 atagcgaatc cttaaaagcc atccaaacat cacaagaggc tatccaagaa gcgcaagcct
    23341 ctcaagcaaa agcattagct gaatcaacaa ataaagttga aaagaatgct gttactgaag
    23401 ataaagctga cagtaaagat tcgaaggttg ctggagtgaa tacatcaaca gacgctaaga
    23461 cggatactaa agcagaaaat gcaggcgatg gcactgctac taaagtagat aaagaggatc
    23521 aaatttctgc aactgaagca attgaaaaag ccagcgttga gcagtctaaa aatgacaatg
    23581 cagctgagac ttcaaataaa gaagctacag tagatgcaga agcgcaacat gatgctgaac
    23641 aacaagtagc agaagcccat gcagaagctt ctaaacaagc tacttcgaac gattcattgg
    23701 aagcgaaagc agaaaatgac agcacagctt cacagtcaga aatgtcagaa ccaaaacctc
    23761 aagaagagaa aaaaggtttc ttcgcacgtt tattcaacct gtaaacttgt gatttgaaca
    23821 caaatttaat actcaattca tatatagctt tgatttaaaa actgattttt ataaacctat
    23881 agaactgtaa tttactttca aatttaatac tcaattcata aaacctgaaa caagctgata
    23941 tactccattc aatatctact ttgaagagag catatcaaac taatttgt
//
LOCUS       DALAGF010000035        23628 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.035, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000035 DALAGF010000000
VERSION     DALAGF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23628
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.035"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(202..774)
                     /gene="raiA"
                     /locus_tag="O2692_001275"
     CDS             complement(202..774)
                     /gene="raiA"
                     /locus_tag="O2692_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCZ4298910.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            complement(835..1509)
                     /locus_tag="O2692_001276"
     CDS             complement(835..1509)
                     /locus_tag="O2692_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCZ4298911.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            complement(1502..2584)
                     /locus_tag="O2692_001277"
     CDS             complement(1502..2584)
                     /locus_tag="O2692_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCZ4298912.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRK
                     RQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNKKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQRMNKLALKRGWIDE"
     gene            complement(2948..3814)
                     /gene="fakB1"
                     /locus_tag="O2692_001278"
     CDS             complement(2948..3814)
                     /gene="fakB1"
                     /locus_tag="O2692_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HCZ4298913.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            3958..4599
                     /locus_tag="O2692_001279"
     CDS             3958..4599
                     /locus_tag="O2692_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCZ4298914.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDIGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            complement(4769..5824)
                     /locus_tag="O2692_001280"
     CDS             complement(4769..5824)
                     /locus_tag="O2692_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCZ4298915.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
     gene            6142..7212
                     /locus_tag="O2692_001281"
     CDS             6142..7212
                     /locus_tag="O2692_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCZ4298916.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            7408..8169
                     /locus_tag="O2692_001282"
     CDS             7408..8169
                     /locus_tag="O2692_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCZ4298917.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            8187..8681
                     /locus_tag="O2692_001283"
     CDS             8187..8681
                     /locus_tag="O2692_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCZ4298918.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            8695..9921
                     /gene="pepT"
                     /locus_tag="O2692_001284"
     CDS             8695..9921
                     /gene="pepT"
                     /locus_tag="O2692_001284"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCZ4298919.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(10110..11234)
                     /locus_tag="O2692_001285"
     CDS             complement(10110..11234)
                     /locus_tag="O2692_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCZ4298920.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAYQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(11659..11979)
                     /gene="ytxJ"
                     /locus_tag="O2692_001286"
     CDS             complement(11659..11979)
                     /gene="ytxJ"
                     /locus_tag="O2692_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HCZ4298921.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            complement(12133..13011)
                     /locus_tag="O2692_001287"
     CDS             complement(12133..13011)
                     /locus_tag="O2692_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HCZ4298922.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            13131..13649
                     /locus_tag="O2692_001288"
     CDS             13131..13649
                     /locus_tag="O2692_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HCZ4298923.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYTKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            13776..14699
                     /locus_tag="O2692_001289"
     CDS             13776..14699
                     /locus_tag="O2692_001289"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCZ4298924.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            14717..15031
                     /locus_tag="O2692_001290"
     CDS             14717..15031
                     /locus_tag="O2692_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298925.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(15348..16376)
                     /locus_tag="O2692_001291"
     CDS             complement(15348..16376)
                     /locus_tag="O2692_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCZ4298926.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(16495..17256)
                     /locus_tag="O2692_001292"
     CDS             complement(16495..17256)
                     /locus_tag="O2692_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298927.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFENGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            complement(17253..18209)
                     /locus_tag="O2692_001293"
     CDS             complement(17253..18209)
                     /locus_tag="O2692_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCZ4298928.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            complement(18196..19167)
                     /locus_tag="O2692_001294"
     CDS             complement(18196..19167)
                     /locus_tag="O2692_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4298929.1"
                     /translation="MKYLLKGNILLLLLILLTIISLFIGVSELSIKDLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFIAATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            complement(19206..19361)
                     /locus_tag="O2692_001295"
     CDS             complement(19206..19361)
                     /locus_tag="O2692_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298930.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLKLWSKNAT
                     INEKYYQ"
     gene            complement(19819..20790)
                     /gene="nrdF"
                     /locus_tag="O2692_001296"
     CDS             complement(19819..20790)
                     /gene="nrdF"
                     /locus_tag="O2692_001296"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCZ4298931.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            complement(20908..23013)
                     /gene="nrdE"
                     /locus_tag="O2692_001297"
     CDS             complement(20908..23013)
                     /gene="nrdE"
                     /locus_tag="O2692_001297"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCZ4298932.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            complement(22976..23374)
                     /gene="nrdI"
                     /locus_tag="O2692_001298"
     CDS             complement(22976..23374)
                     /gene="nrdI"
                     /locus_tag="O2692_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCZ4298933.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
BASE COUNT         7775 a         4109 c         3387 g         8357 t
ORIGIN      
        1 ttttacgatt ttcgactcgg actatacaat agactgacat actattatta acttaacatt
       61 caaatatata catccattaa cattagcata gtcactatgt ttcattcaac aaattacatt
      121 atcgaactat gaaatagtca taatttgctt ttggagtata aaaaagcact tgtgcaaaaa
      181 cacaagtgct ttaaacttaa tttattgttc actagtttga atcaagccat atttaccgtc
      241 tttacggcgg taaacgatac ttgttccatc agtttctctg tctgtgaata caaagaagtc
      301 atgacctaat agattcattt gtaataccgc ttcttctgaa tccattggtt ttaagctgaa
      361 ttcttttgaa cgaataattt ctatctcgtt atcatcgtaa gcgtcattat caacttgtgt
      421 ttcttgcatt tcttgtaatt cggcaacaaa cacttcttga tctcctcgat cacggctctt
      481 acgattaata cgtgttttat attttcgaac ttgtctttca agtttattat taattaaatc
      541 aatacctgcg tataaatcat cgtttcgctc ttcagctctt aacgtaacat ttttcaatgg
      601 aattgttact tcaattttag tagctgaatt tgaataagtt ttaactttaa catgcgccac
      661 tgcatttggt acgtcattaa aataacgttc caacttacca attttttcct caatatagtt
      721 gcgaatagca tctgtgatag tgaggttatc tccatgaatt tcaaatctaa tcatagtaat
      781 ctctccttaa acctctttat tggtaactct ttattatatt ttaacatttt tacgctatcg
      841 tgcaaacgca aacactttga attttctgat atttttagca tataatttac accctgcacg
      901 atgaattgtt aatccagttg tataaatatc gtcaacgagt aatatttcct taccatttaa
      961 atctaattcc gtatcaataa taaatggatt ttcatctgcc agacgctctt tcttagttaa
     1021 atgagactgt tttggtctat ttgacatctt taaaatctta tcaaagcgaa tccctttagc
     1081 ttttagtact gcttctaccg ggttaaatgt tctagataaa tcatgtgccg gcgaagaagg
     1141 aattggcaca atatagtcat aagatgtttg tggtatttca atcaaatgtg ccaataattc
     1201 acataaataa tagtctttca aaaatttata ctgatgtatc atctctttca ttaaaccgtc
     1261 atattgaaat tgacaatata attgttccat taaattaaag tgtgccgata gaaacttgca
     1321 gtctaaacaa tacgcttcat cttgatttaa gtgttttaag caccttgaac atcgccttgc
     1381 tttaatatca agtttaatat tgtcccaatt ctctttgcat ctgtcacata atctattagg
     1441 tttcttgaac aaattataaa tggttatatt ttcatataac ttagcaccac aactcaaaca
     1501 attattcatc aatccaacct ctttttaatg ctaatttgtt catcctttga atctcttttt
     1561 tagcttgaat catattcata cttacccctt catgaaaaaa caatactttt ccagtaggac
     1621 attctaattt tcgtccaaca cgtccagcaa tttgtattaa agcctcttga gtatattgat
     1681 gtgcatcgat aacaacaaca tccaaatttg ccattgtaaa tccacgttct aatattgtcg
     1741 tagtaaaaat gacatcgaaa tgtccattcc ttaattgttc aactttttca aagcgaaaaa
     1801 catcctcgct atggacgtat gttaatttag taattttctg cttataaacc gaaaatgttt
     1861 taatcattgt ttctatattg ttaaaaaaca ccagtgtata acgttgatta ttaatttgat
     1921 cttgtaaaat tcggtataac attttctgaa tcttcttatt attaagtttg aaataacgat
     1981 atttaggaac tggaagtgat tttttatgaa agcgagctgg caatttaatt atattttcgt
     2041 ggggaatctc tgacagaagt tgtttcggtg gtgttgctgt catataaatt gttgcatgtt
     2101 caactttaga agatgacttc aatgcttgtt gtaaattttt atccattgat aaaggaaagg
     2161 catcgacttc atcaataaaa atagtatcaa agtgctgttt gaatcggtaa agttgatgca
     2221 ctgtgcatac aacaaaatgc ccttcaaatt gttgtcttga ttgctggtgt agtatgtcta
     2281 tatcttcatt aagaaatgcg tctttaatac gtttactaat ttctacaaca acatctacac
     2341 gtggtgacac aatagctata ttatctccct gtattcttgc atattgaatg ccttgaaaca
     2401 tcatttctgt cttacctgca cctgttacgg catataacaa aatcgtttgt ctctttctaa
     2461 tggctcgaac aatatgttct gaggcataag actgctgttc cgacaattca aatgagagat
     2521 gataatatgc ttgtgatgaa ctttgcgaac tctctgttat tttatatctt gttacattat
     2581 ccattcgacc cagtgatata caatttctgc aatatacaat tttcttggaa tgtactgagg
     2641 aatcatactg ataaaaatgc tttggatttg tactttcaca ttgaacacaa taatagtttg
     2701 aattttgttt gatgacacct gttacttctt ttttaatttt ttcattagta agtgaagtat
     2761 gaggatgaat taatttcccg taatgtttaa tgcttaaaac ctcatttcaa cgtaaaaaag
     2821 agcctaatat tataatgatt ttcttatatc tttagcattt tggctttcaa atgatgaaca
     2881 tttaaagcca tgtatacgag agataatttc caattacaat atagactctt ttattaaaat
     2941 tttataatta tgttaatctt atttttctgc caacatagcc taaacctaat ccaccagaac
     3001 ctaaatgtgc tgcaacaact ggaccaaact cagagtatgc tacttgataa gctgaaggac
     3061 aatcatcttg taactttttg tataacgctt gaccatcttc gaaatgatct ccatttatga
     3121 caaataaagt tacttcttca aagtctttta caatatctaa tactttcttt tctaatgttt
     3181 gaatggcacg ctttttagta cgaacttttt cttctggtat aatcttgcca tcttcaaact
     3241 taagaactgg cttcattttc aataatgttc caacccatgc ttgagcacca gtaattcgac
     3301 cacttttttg taaattcttt aagtcatcaa caattaaata tgcacctgtg tgttcacgca
     3361 tattagttaa atcatcaata atttgctgtg gctcgtatcc ttcttcaacc atttcaatag
     3421 cacgtaatac ataacatcct tcaatcattg ctgcaagctt actatcaaat gcatgtacat
     3481 taacaccttc aaccatttcc cctgcttgat aactagattg ataacttcca ctaatcccac
     3541 ttgataagca aatgacaatg atatctgtgt aaccttgatc tcttaacatt tcatatttag
     3601 taatccattc gccaattgct ggttggcttg ttgttggaat cgtttgagac gatgccattt
     3661 tattataaaa ttcttcaatt gctatttcat tactttctgt aaagttcttc ccatcatcaa
     3721 aagtcacact taatggcgct atttgaatat tatatttatc gattaagtcc tgcgacagat
     3781 aacttgtaga atcggtcatc acagcaattt tcatagtcgt cctcctaaaa tatactcata
     3841 gcaatgatac aatatttcag ttaaaaagaa aatggcaatc aaacgattta tagtcatgtc
     3901 attttcagtg tataatgaag attaaataat cttaagctta tgaattaagg agttattatg
     3961 acacaaaata tcattactgt taaaaaagaa cacattattg aaaatgtgat tagtaaatca
     4021 agattcattg ctcatatcaa acctgttcaa aatgaagatg aagctaaagc atttattgct
     4081 gcaataaaaa aagaacataa agatgccact cataattgtt ctgcatatac tattggacct
     4141 gaaatgaata ttcaaaaagc gaatgatgat ggtgaaccga caggaactgc cggtgtccca
     4201 atgttagaca tattaaaaaa gctcgatgtt cataatgcgt gtgtcgtagt aacaagatat
     4261 tttggtggta ttaaattagg tggtggcggt ttaattcgcg cctatagcgg tgctgttaga
     4321 gacgtcattt atgatatcgg gcgtgtcgaa ctacgagaag cagtaccatg tatagtaaca
     4381 ctgaattatg atcaaactgg taaatttgaa tatgaattag cttcaactca ttttatgtta
     4441 agaaaccaaa cttacactga taaggtaagc tactatatcg atgtcgttaa aagtgactat
     4501 gaagcgttta ttaacttttt aaatcaaatg actgcaggaa atttcgattt gactgaagaa
     4561 actccaaagc agttaccttt cgatatacca actgaataaa aataaaaagc actatcataa
     4621 gacggtaaat atcaattgcg tattattaaa cgacaattga agtacacgtt tatggcagtg
     4681 ctttttatat ttgaaattta atacaaaaaa attgtaaaga gtaaagccat ataatacaaa
     4741 ttatcccata cagctatgct ttcattccct attcctcttt atgagatgac ttacgactaa
     4801 ttaaatttaa tattggtcgg tagttgttat ctattaatcc tgtaaattca acaattaatt
     4861 caatactaaa tatgattaat acaaacatta atacaacacc taatggtggc gatacataca
     4921 aaataatgct cgaaagacta aataaaatag agattgaata gattaataat actgtttgtc
     4981 tatgtgtgta gcctaaagct aatagtttat gatgcaaatg cgatttatca gcttgcatta
     5041 tatgctgccc ttttttcaca cgtcgaatca ttgcgaacaa agtatcaatg aatggaactg
     5101 ctaagataac aattgggaag aacaatgcaa taattgtaat atttttgaat ccgagtaaag
     5161 aaaggaatcc gatgataaat ccaatcatta aagccccact atcacctaaa aatattttgg
     5221 caggatggaa attgtaaaat aaaaacccaa ttaaagagcc taataaaaca caacaaatca
     5281 tcgttatgaa aatattagct tgtaaaattg caatgaaccc tattgtaatg agtccgattg
     5341 cagaaacacc cgacgccaaa ccatcgagtc catcaattaa gttaattgca tttgtaatag
     5401 caacaatcca aatcacagta attggaatac taagaaatcc aaaatgaatc gttgtaccca
     5461 ttggcaacga aataaaatca atagtcacac cataaaaagc aactactaag gcagcggcaa
     5521 tttgaccagc caattttata tacggtttca aatcgtagat atcatctaca agcccaagta
     5581 cgtacataat aatcgcacca ataataagtg gtttgatttc tgtttcaata ggatgaccaa
     5641 tccaaatacc tattaaaaat gaaaagagaa tcactgtacc acccataact gaaataggtt
     5701 tagtgtgtac ttttctaaaa tttggtttat caactaaatt taatcttttc gatattgcaa
     5761 taacaattgg tgttatcgtc aaactgacaa tcattgttac tgcaactagt aataatgtaa
     5821 ccatttattc accttcatcg atattaattg tttttaacgt tatatttatt taatattgtc
     5881 tttatagaat gatatatcta ttttctacgt atatcttata gctttattat aaattaattc
     5941 gttcaatcaa tgatatcata tttacaacta tacgattcat ttcttatata tcgcctatta
     6001 ttcatttttt catcatatca tttaaggcaa cattttaaca tcaaagtcta tatcatacat
     6061 taattttata tgtaaactaa tgacaaattg taagttttca cttacaatag ctagtggtca
     6121 attaaaggag tcgattcaga catgttcgaa gcatttatat acaatatatc tgttatagtc
     6181 gctggaatct atttattcca tcgcttacag tattcagaaa acaaacgtat ggttttttct
     6241 aaggcttatg tcactgtttt aatgacaatc gtatcattat tattatctgt ctaccctatc
     6301 ccttatcgtg aagattattt aattcactta acattcgttc ctttattgtt tttaggtaga
     6361 tttacaaata tggtctatac attatctgcc acagtaattg tagcaattgt tgaaattgta
     6421 gtatttaaca actcgattat gtacggtgtt acgttgattg ttattgctgc agtaacaagt
     6481 gctattggac catttttaaa acaaaatgat gtgctatcat tattaatact taatgttgtt
     6541 acaataatca ttttatttgg tgtcgcgtta gtgagtccta tttacacact atcggaagtt
     6601 atcattttaa taccaatttc attaattatc actttagctt cagcgataac atttgtcgat
     6661 atttggcatt tcttctcact cgtaaatcgt tatgaaaacg aagataaata tgattactta
     6721 acaggcttag gtaatgtaaa agaatttgat agacatttaa atgaaatttc acgaaaagct
     6781 gaaaaagaac atcaaagtat cgcattatta ttaatcgata tcgatggatt taaagatgtc
     6841 aatgatacct actcacacaa atcaggtgat gctgtattaa aacaaatgtc tcaattactt
     6901 aaaaactatg tgccgaatca atttaaaatt tttagaaatg gtggcgaaga gttctcagtt
     6961 gtcattcaca attattcact tgatcaaagt gttaaattag ctgaaaatat tcgttcgggt
     7021 gttgaaaaat catcattcca tttaccaaac aaagaagtta ttaaattgtc agtgtctatc
     7081 ggtgttggtt atttaacaga tgacgatcca aaatcacagc gtaaagtgtt taaagatgca
     7141 gacgatatgg tgcatgtagc caaaaatcaa gggcgaaaca aagtaatgtt taaccctatt
     7201 atcaatttat aacaacgaca aaactgttaa tcctttgcta ttattaaatc atagtataat
     7261 agcaaagatt aacagttttt cttatttaaa tttgaaccat acaagttcat catactaaca
     7321 caaagtctca ctattgaaag ttgtaacaac aagttgtaaa cttcaattgt tcaaataaat
     7381 aacacatatc attggaggtg aattgatatg ccagattcaa tcacaattat agatgaaaac
     7441 aaagtgattg atgttgtatt aattgcaggt agaattttac ttgaatcagg tgctgaaaca
     7501 tatcgagttg aagatacaat gaaccgtatc gcacatagtt atggtcttca taatacatat
     7561 agttttgtca gttcaactgc aattattttt tcattaaacg atcgaacaag tacaagatta
     7621 attcgtgtac aagagcgtac aacagattta gagaaaatcg ctttaacgaa tagtctttca
     7681 cgtaaaatat caaataaaga actcacaatt gatgaagcta aatctgaatt tattcattta
     7741 cagcatgcat cattacaata ttctttttta acgaatttct ttgcagctgc cattgcatgt
     7801 ggcttcttcc tatttatgtt tggtggtgtc gcatcagatt gttggattgc agtcattgct
     7861 ggcggatccg catttttaac attcagcttt gtgcaacgct atatacaaat taaatttttc
     7921 tcagagtttg ttgcagccgc tgtggtcata tccattgcag ccacatttac taaattaggc
     7981 attgcaacca atcaagatat cattactata gccagtgtca tgccacttgt ccctggtata
     8041 ttaattacca atgccattcg agatttatta gcgggtgaat tacttgcagg tatgtctcgc
     8101 ggtgttgaag ctgcattaac agcattcgca atcggtgctg gtgtcgcaat cgttttatta
     8161 atcatttaag gaaggactgt taaagcatgc tattttattt atttcatttt acaatcagct
     8221 ttatatcaac agtacttttc tctatcattt tcaatgcacc caaacgcctc ttagtagcat
     8281 gtggatttgt gggtgccatt gcatggacga tttaccaatt aacggtagat ttagagtttg
     8341 gaaaagttgg cgcttcattt ttgggaagct taattttagg cttaatgagt catactatga
     8401 gtcgcagata taaacgaccg gtaattatat tcatagtgcc aggcattata ccattagtac
     8461 ctggtggtgc agcttatcaa gcgactcgtt ttttagtatc aaatgattat acaagtgctg
     8521 taaatacatt tttagaagtt acactgattt caggtgcgat tgctttcggt atattagttt
     8581 ctgaaattct atattaccta tacacacgta tcaaacaact gtatggtaaa atcaaaggta
     8641 agacatataa aaaatcttac aacatgaata atagagttta aggggtcgaa tataatgaag
     8701 aaccaattga tagatagatt aacaagatat acgacaattg atacgcaatc tgatccaaaa
     8761 tctacaacaa caccttcaac tgaaaagcaa tgggatttgt tacatttatt agaaaaagaa
     8821 ttacaacaat taggattgcc aactgattta gatgaaaatg gctacttatt tgctacatta
     8881 gaaagtaaca tcgatgtcga cgtcccaaca gttggatttt tggcgcatgt tgatacatca
     8941 cctgatttca atgcttcaaa tgttaaaccg caaattattg aaaactatga tggtaagcca
     9001 tacaaactag gcaatactaa acgtgtctta gatcctaaag tatttccaga acttaacagt
     9061 ttagttggtc atacattaat ggtaactgat ggtacatcgt tactaggtgc agacgataaa
     9121 gcgggtattg tggaaattat ggaagctatt tgttatttac aagaacatcc agaaattaaa
     9181 catggtacca ttcgcattgg atttacacca gacgaagaaa tcggtcgtgg tccacataaa
     9241 tttgatgttg accgcttcaa tgctgatttt gcttatacta tggatggtag tcaatatgga
     9301 gaattacaat atgaaagctt taacgctgct gaagcggtta ttacatgcca cggtgtaaat
     9361 gttcatcctg gttcagctaa aaatgcaatg gtaaacgcaa tacgtttagg tgaacaattc
     9421 gatagtttgc tacctgatag tgaagttccg gagcgaacag aaggatacga aggcttttat
     9481 cacttaatga actttgaagg aactgttgaa aaagcaactt tgcaatacat tattcgtgat
     9541 catgataaaa aacaattcga attgcgtaag aaacgtattt tagaaatacg tgacgatatc
     9601 aatgcccatt ttgaaaatta tccagttaaa gttgatatat cggatcaata tttcaatatg
     9661 gcagaaaaaa tattaccatt gcctcatatt attgatatac ctaaacgtgt ctttgccaaa
     9721 ttagatattc cagcaaacac tgaacctatt cgcggtggta cagatggttc acaattgtca
     9781 tttatggggt taccaactcc aaacatcttt acaggttgcg gtaacttcca cggcccatat
     9841 gaatatgcat ccattgatgt tatggaaaaa gcagtacaag taatcattgg aattgtagaa
     9901 gatatcgctg aaaatcacta aaaaccaacg attatatact agttcaactc aggcttaatt
     9961 aatatataaa aatcacgcta acattcagtg caatgatagc gtgatttttc atatttttta
    10021 cgtatacttt cgatcttcta attcgttttg ctaaccatca taaatgttat taaccatcat
    10081 gacagcatca ccaatttagt ccttctttac taaacctcaa cattaaaatg tgttttcaac
    10141 agttttaaag actgaaccgt ataatgccta atttgtaacc ccattttaaa gtcagtataa
    10201 gtttctggca tatcgataaa tgtattaaac attgctgtaa ccccttgtga ttcaaataaa
    10261 tcaaactttt cagcagttgc acaaattgca atggcaacct tttgatgttt atgacaaagt
    10321 tctgcaatac gcaatgttgt cgtttctagc aactgatcat tttcatttaa tccttctcca
    10381 aaaataatta aatccgcttg ttcaactaaa ttttctaaat gtgttagttg gtctactaat
    10441 gcatgactgg ttaatatttc agcttgatac agtccattca agactgctgc aattccacca
    10501 ccagcaccac cacgttcaac tggaccaatt gcaattttca attcactttt aaataactca
    10561 ctaaaatacc aaattaaatt atcgatttct gctgcttgat tatgattcaa ctgatacgca
    10621 tcataagttt gcatgatttc actttgctta ccatataatc gacttgaaaa atccgacatt
    10681 acttgaattc ttgctgtttc cattttaggg tgtaagttcg acatatccat acgacgaata
    10741 tatttaatta caccagcacc ttgtctcata tctacgacac gcccttcgtc atcatagaat
    10801 tgagcaccta atgcttgtaa catacctgca ccagcatcaa aactatcaat cccacctagt
    10861 gaaattacaa catgttttgc gtcgttatct aatgcatgtt taatcatttc tcctaaacca
    10921 taacttgttc gttcaacaat tggtttttta ccttttaaaa ataaattccc ctcgataacg
    10981 gtcatccctg tatcagtttg tccgtaaaca ccttcaactt cattcatatc tgcatcatgt
    11041 actggtatac gatacttttg cccagattgc cataaaaata cagaatctaa taattcatgt
    11101 cttccattaa acaatggtac ttgaactaca tctgcagttt caatttggct tgcaactgcc
    11161 tcttcaacat atctattagc ttgataactt gaaataattc catgaaactc atccatggct
    11221 actaatactt tcataacctt gaccacctta ctttattatt tgttgtgata cgttttttat
    11281 tcataaaaga ttaatcttaa attttagatt ttatctcgtg attatatgcg aacatttaac
    11341 atgacatgat tattaattat tccagtaaat aacaatatgc ttcttttggt ttagcatata
    11401 tttcctatta aactaactta caaatcatgt ttaaattttc ttattggatt cgtcgttttc
    11461 caaaatattt tcaataaaaa tactcgaata tattatacat atgtttgtaa aaaagtacta
    11521 acttaactgc ttctaaaggt gacataaaat aaaaggtcag aacatcatta tagccaaact
    11581 actctgattc acacgaattt aagttgtgct attcacgtct agtagtgtaa ggcaaaatgt
    11641 tccaacccta tagtttcatt attcttctgc ttgtgctaat gacgacacat tgatatcacc
    11701 gtggtctcga ttccaaacca tttcaccatt tacaaaataa aatgcttgag gtgattcatg
    11761 tttaacgttc gtttttttag caatataatc tgacaaatcg cgttcttgtt ggacaatcaa
    11821 ataataaccg tccatatcgc gttcatataa aaatttatta aattgatcgt acgcatttgc
    11881 cgatattgga caagtttcac tatgttttaa tacaaaaaca tatttatttt cctcaataac
    11941 ctgttcaaat tggtcaattg aacttagctt tatagccact ctattcacct ctaaaaatat
    12001 ttatcacatt taaatagcat ctgcgtgaca tttttataca tatagttaat atcaatgttt
    12061 accattatac acatagtaag ttgacatgtt aaccataaat tcaaaatttt taactttgaa
    12121 aaatgattat agttattttt cttctaattt ttcaagcttt ttttcaaagg ctttatcgcc
    12181 gtgagttata tctataccct ttttcggcaa cttatctaca tcgacttttg ataacttctc
    12241 actaaccttt attgtttcaa tacgtgtatt ataatagttc tgcagactgt aatacatttc
    12301 tattgcgttt ttccttgcat tattaacatg cttatcacta atattagttt ggttaatatc
    12361 ggtaatttgc ttttcaatca ttggcataat gtgattttta atcgcgcctt ttacttcatt
    12421 ttctttacta tcatctaggt tcttcttcgc agtatctctt aacgctttat tattatgttc
    12481 taattttgtc gtaagattag ttgcttcact tttattatca gctaatttaa tttctgattc
    12541 aactttgtat ctattttttt caaattgttt cgtataatct aaaatgtctt cgttatactt
    12601 gatagattga ttacataaac ctatgaattt ttttaattga tatatcgcat ccttcttctc
    12661 atttgcaagc gtcatatatt ctttttttaa tttcttaact ttcattgtat catcaggcaa
    12721 attttttgct gacttataat atgcttcaaa tttaggtatg agatggtttt taacatcttc
    12781 ttgtagtgcc ttaaattctt tactattttt atcagttgta tcagttttac ttagatgatc
    12841 aatttctttc aaatgtatat tatccatgac atgttgtaat tctttttgtt tttgctcaac
    12901 atctttaaat tgcttagtaa attgatgttg tgcctctttt tctttattac cacaagcact
    12961 tattactaca aataaaatcg ctaaaacagc gataataaca atttttttca tgattgcact
    13021 ccttaacaga ttcattatac aaaaacatgt ttattatgaa aatgaattat gttatagtat
    13081 tccagacttt atcgtgatta aattaatttt caatatcaaa ggagtttatc atgtattcaa
    13141 atgttcaacc attcattaat agtgatttaa ctaattgttt tcaatcacaa tacactgaaa
    13201 ttgaatcatt attattcaac ctcttagaca gcccagtaaa atatacaaaa catattggcg
    13261 gtacgcatca ttttaaatat tcaacagagc caattttaga tattttagta ggtgtagaaa
    13321 atttgcatga tattacatcc ttagatgaaa aacgcttaaa ttatgtaggg ttttatcgtt
    13381 tgcatcataa atatactaag aaagttatga tggcgaagtt taacaatttt catgaactaa
    13441 aacaacaggt gcgtctacat atcattcaaa tgaataccca aacatttgaa tcttatcttg
    13501 cacttgatta cctcttatca actaatgatg atattgcttt gcaattcaat acaaaaaaac
    13561 accaaatatt gcaacatagc ttaacaatta gacaatatga gatgcaaaag caacaatact
    13621 ttgaaaaact atgtaaaaaa tacttataac gacaataatg ccgtatttta taattatact
    13681 aattctaata ctttcttttc aattttcgca aatgaatttt aaaattggta taatactata
    13741 tgatattaaa gacatgagaa aggatgtact gagaagtgat aaataaagac atctatcaag
    13801 ctttacaaca acttatccca aatgaaaaaa ttaaagttga tgaaccttta aaacgataca
    13861 cttatactaa aacaggtggt aatgccgact tttacattac ccctactaaa aatgaagaag
    13921 tacaagcagt tgttaaatat gcctatcaaa atgagattcc tgttacatat ttaggaaatg
    13981 gctcaaatat tattatccgt gaaggtggta ttcgcggtat tgtaattagt ttattatcac
    14041 tagatcatat cgaagtatct gatgatgcga taatagccgg tagcggcgct gcaattattg
    14101 atgtctcacg tgttgctcgt gattacgcac ttactggcct tgaatttgca tgtggtattc
    14161 caggttcaat tggtggtgca gtgtatatga atgctggcgc ttatggtggc gaagttaaag
    14221 attgtataga ctatgcgctt tgcgtaaacg aacaaggctc gttaattaaa cttacaacaa
    14281 aagaattaga gttagattat cgtaatagca ttattcaaaa agaacactta gttgtattag
    14341 aagctgcatt tactttagct cctggtaaaa tgactgaaat acaagctaaa atggatgatt
    14401 taacagaacg tagagaatct aaacaacctt tagagtatcc ttcatgtggt agtgtattcc
    14461 aaagaccgcc tggtcatttt gcaggtaaat tgatacaaga ttctaatttg caaggtcacc
    14521 gtattggcgg cgttgaagtt tcaaccaaac acgctggttt tatggtaaat gtagacaatg
    14581 gaactgctac agattatgaa aaccttattc attatgtaca aaagaccgtc aaagaaaaat
    14641 ttggcattga attaaatcgt gaagttcgca ttattggtga acatccaaag gaatcgtaag
    14701 ttaaggagct ttgtctatgc ctaaagttta tggttcatta atcgatactg aaacacgctg
    14761 tcgccattat tttaccgaag aagatattat tgctattaaa tttaaatgtt gtaataaata
    14821 ctatccatgc tataagtgcc ataatgagtt tgaaaagcac gccattaagc gttggtctga
    14881 gccttcattt aacgaaaaag caatcttatg cggtgtatgc aaacacgaat taacaatcaa
    14941 tgaatatatg atggtagagc gttgtccaaa ttgccaatct cggtttaaca atcgctgtaa
    15001 atatcactat catatttatt ttgaaattta aacaacatag tcaaaacatt atagggagtg
    15061 ggtcagacat gataaagcac cacttctgta gaattccttt ttgaaattct ctatgtttgg
    15121 gccccgccaa cttgcattgt cagtagaaat tgaaaatcaa atttctctat gtttggagcc
    15181 cactagtatt gaaatgaact tgttacaagt acatgttcat tcaaccaacg actgacaaca
    15241 gaacatttta gagtctagga cattgattga cgtcccagat ttcctttttt aaagaaaaga
    15301 ggggagcaat gattaagacc tttaaccatt gttcccctct tttaaaatta ttttacaact
    15361 ttatcaagtt cctcaatttg tttaattgta gttgtagttg atccagctgc aaagtaccat
    15421 aatttaggat ctaaattata tactttgtct tctttaattg ctttaacatt ttttaataca
    15481 ggattattta atgcttgttt cgcagttgat ttaccactta tcgcttgacc tctatccatc
    15541 gctaaaataa catctggatt ttctttatta acatattcgt tagaaacatt ttgtccatga
    15601 ttgctattac ttactttttt atcaactgca ttgaatccta atgtatcgta aactaatcca
    15661 ccaaaacgac ctttaggtcc aaatgttgat aattcacctt cgttaactag taaatacata
    15721 acagttttat tgaagttttt cgttttatct ttcattgaag caattttgtt atctaaatct
    15781 ttatttaatt ctttagcttt atcttcttta tcgtaaattt ttccgatatt ttcagtgttt
    15841 tgtttcattg aaccaattaa gttcttttca tctgcaccaa cataaacaat tttcgcttta
    15901 ggtgcagctt ttttgaattc atctaaattc ttttgattag ctgtacgtcc agagataaag
    15961 attacttcgg gtttcgtcgc agcaatttta tcaaaattca cttcttttaa attaccaacg
    16021 tttgtatatt tatcatcttt aaatgattct aagaaattcg gtaatgactt accgccttcc
    16081 cctttaggta atgcttttac tttatctgat aagcccattt ctttcattac atctaatgcg
    16141 ccatagtcta acacaactgc atttttagga ttttttggta cttcaacagt ttctttaact
    16201 tttttagcgt cactaccatc ttttttctcg ccacgcattt tatagttatt ttcaattttt
    16261 actgtatctt tagaaccttt agtttctgat tttgattgtt ctttatcaga attgttaccg
    16321 catgccgcta ataaaaacat tactgctaat actaaatata agactgtttt cttcataata
    16381 taatattctc ctcttttggc ttaatctgtg tgcttgatgt gaaatataaa taaatgcttt
    16441 acgtttaacc cttctttaat tggcgcatca cccctttaaa gtgtctccaa attatcaatc
    16501 cttaaaatat aaacaaatcc tttgatttct tatatgttca atttgaatat tcatgtcata
    16561 taaatcactt aaaacactac tttgaatgac attatctttc aaatctgact taactagttg
    16621 tccgtttttc aatgctacaa tctgatctga ataacaggac gcaaagttaa tatcatgtaa
    16681 cacaatgaca atcgctttat tcattttatg cgctaacatt tttaacgttt gcataatttg
    16741 aacagcatgc ttcatatcta aattatttaa tggttcatct agcaaaatat attcagtatc
    16801 ttgtgctatt gtcattgcaa tgtatgcacg ctgacgttgt ccaccagata atgacttaat
    16861 attacgattt ctgatatctt gtagttgcaa caaatctagc gcatcattga caatatcatg
    16921 atcctctttc gtcaaacgac ctttagaata agggaatcgt ccaaagttta ccaactgctc
    16981 taccgtaata ttcatttcag tatggtttgt ttgttttaat atagatattt ttttcgacaa
    17041 gtcattattt ttataatcag acatgagctg tccatctatt ttcacttcac cgttctcaaa
    17101 acgaattaac ctacatatcg ctgaaagtaa agtactctta cccgcaccat taggtccaat
    17161 taaagaagtc aatttacctt tttcgatatc tatgctaata tcttccaata tcatttgatt
    17221 atttatagtt ttagttaaat tttcaacttg aatcattgcg catttctcct tctaactaat
    17281 agataaatga aataacttcc accaatcaaa tcaataagta tactcatttc tgtcgtagct
    17341 tcaaacacat tttcaactac ccattgcgca ctaaataaac taatccaact caagcaaatt
    17401 gtcgcaatta aaatatactt atgttcatac gtcttcatta gttcatgcgc tagatttaca
    17461 gttaataaac ctaaaaatgt aattggtcct actaatgcag ttgacacaga aactaaaact
    17521 gagactatta caagtagaat tcgcgttaat ttttcatacg atatcccaag attaattgct
    17581 tcagcacgac ctaaaagcaa tacatctaaa taaggcaata gtaaaattgt aatgactaat
    17641 aagattacta atagcactgc tgagaaagta actaaattcg aattagaagc attaaaatta
    17701 gcaaacatac tactttgtat tgctaaaaat gactcaggat ccataatcag ttgaataaaa
    17761 cctgttatgc ttctaaaaaa tgttcctaaa aggacaccaa taagtaagat aaaataaatt
    17821 gaaaaatgtc cgattttaaa gataccttgg aataggatta gtgcgaataa caccatcgtt
    17881 ataagtgtta atataaagtt caaatataca ttagtaaccc ataccgattg aattccaaat
    17941 aaaaagactg gcaatacttt gataaataaa taaactgcat ctaaccccat tattgatggt
    18001 gtcaatagac ggttatttgt aatcgcttga aaaatcacca ctgaagttgc aatggcagca
    18061 cctactaaaa taattaatat gaactttctt aaacgacttg aaaattgata ttcaaatatt
    18121 tcaaaatcaa tacctacaaa taagtacagc atactaatta ataacgttac tgcaattaaa
    18181 agcattattt ttttattatt gttgcgcata atttttccta cctttcataa gcaagataag
    18241 gaaaatgatt gttccaaata cacctattgt taaaccaata tttatttcat atggataaac
    18301 aacaattctg ccaactatat cagaaaataa tacaaagatg gcacctaaca tcatcgtatg
    18361 agggatagca tttttcaaat gatcacctcg ataaattgaa ataatatttg gtattactag
    18421 tcctaagaac ggtaatgttc caacagtcac cactaccaat gctgtaatag ttgcagcaat
    18481 aaacaatgcg atgttaatta atttttcgta actcaaacct aaattattag taaagtcttt
    18541 acccattcct acaatcgtga aatgattagc aaaaagatat gtcaatgcta aaagaggaat
    18601 acttaaatat aaaatttcat agcgtccact tgtgataatg gcaaagttcc cgttaagcca
    18661 gttaccaatg ctttgaacag cattcgtgcg caatgagatg aaggttgtga aacttgcaac
    18721 aatcccaccc atcataattc ctaaaagcgg tacaaaaatg acatctttca cttttataaa
    18781 atcaatgatt ttaacaaata aaaacgtacc gcaaatactg caaataacag caaatactag
    18841 ttttaataaa atatgaccgg ttggaaagaa caataaagca attaaaatac ctagtttagc
    18901 ccattccatc gttccagctg tagtcggact aacaaactta ttttgcatca tttgttgcat
    18961 tatcaagcct gctaaagcca acgaacttcc agcaattaaa atactcatcg tccttggtat
    19021 tcggcttgag aataaaatat tccgctgtga ctcagttaaa tgtagtaaat ctttaattga
    19081 tagttcactc acacctatga acaacgaaat aattgtcaac aatattagta atagaagcaa
    19141 aatatttccc tttaataaat acttcattac aaatttttct ccctttaaat taatgataat
    19201 cattatcatt gataatattt ctcattaata gtagcgtttt ttgaccataa tttcaatagc
    19261 ctttcaaata atttagtgtc aattttattg atatttgcaa ttaaactgaa atttaatttt
    19321 tcggaatgtg tattttgttt acttaaagta aaatagaaca cttaagattt tgatgtctgg
    19381 gaatagtgga aatgataaaa actactaatg attgattaag tagtggttct taacattagc
    19441 ctcagctaat gtgtacttaa aaataggaat acatgagtaa aactcatgtg gaagtgataa
    19501 tttctaaagg aaaaagtttt tctttatgtt ggggccccgc ccgaacttgc acaatattgt
    19561 tagctgactt tccgcctgct tttatgttgg ggccccgcca acttgcacaa tattgttagc
    19621 tgactttccg cctgcttctg tgttggggcc ccgccaacta ctgccaatat aaatagcatt
    19681 gtacatctta gagcattgat ttcttactca gactcataat gagttagata cacataaaaa
    19741 aagaccttca acatgtagga gacaagacga aacgaatcgc tatttccctt tacatgtgaa
    19801 ggtcttttaa tttaattgtt atttgttgtc aaatacaaag tcatcatctt gtaatgcttc
    19861 tacgtttaat gctaatacat aaccatcacc ttttactgag aagaagtcat ggtttttagt
    19921 tgttgtatct aaggcattct caatgattgg gttaaattca cgttcctcaa aataaggttc
    19981 aaagcctaag tttgaaagtg ctttgtttcc attatattta acatagttta gcacatcttc
    20041 agtgattcca agatcatcgt ataacatttt tgtgtatgac tcttcattta aatacaagtc
    20101 atttagcaat ttatacattt cttgatctgc tttttgtttc tcactttcag atagttcatt
    20161 tcgtaaatgc tgtgcatcta aaccggtaaa tacaccatga atagattcat ctaaaagaat
    20221 tttacgaatg atttcacctg atgtcgtcat tttcccttga ccagcaagat atagtggata
    20281 atagaaacct gagaagaata agaatgtttc taaaaataca ctcgtaactc tggccatata
    20341 ttggtcgtat atcgaagctt ctttacccca aagtttgtga taattagcaa caattttatc
    20401 agatttatat tttaaatggg gttcctctaa aacccattca tctaataggt agtttgtttc
    20461 actagatggt aatagtgttg tgaaaatatg tgaatagctt ttcgcgtgta tttgctccat
    20521 catcgccata aatgaataaa ctgctttttt ccttaagtca gtcgtatgta gcataactaa
    20581 aggcatgcca tcatctgctt gatgtgtatc taagcctgtt aaaccagcta atgctttttt
    20641 aaatgtgtct tgttcagctt cagataaagt cttccaactt gcaatgtctt ttgatacttt
    20701 aaattctgtt tcaacccaca tttgagatat attttgtctc caaaacatat tcgtcatatc
    20761 ttcttgtgtg ttccaattaa cagctatcat gaatataatt tcctcctgta atgttactca
    20821 ataattgaca aatacagaca tagcagtcta atcatctgct aattcagagc agatgattag
    20881 ctgtttgtca ttttcaacat ttaattgtta gatagaacaa cttgtacatt cttctacact
    20941 taataattta tttctagtat agtaaagtga ttttaatcct ttatagtgcg catatacata
    21001 taatcttgct aactcacgtg tagaaatttc agaattaaca taaaggatcg ttgagatacc
    21061 ttggtcaata tgcgtttgaa ttgtcgcaat taaatcgatt aatttcatct gatcagtatt
    21121 gaatgctgat ttgtagtacc acattgtttg tggtgataag aatggcatag ggtaaaatgt
    21181 ttccgcatta ccataagtac gacgttcaat ttggtcaacg attggcatta cagaacttgt
    21241 tgcattttga acataagaaa tactttgtgt tggagcaatt gctaatctat atgcatgata
    21301 taaaccatat tgttcaacat cttgttgtag tttcttccaa tcctcagaag taggaatagc
    21361 cataccatcg aataattcac gtactttttc gaattgaggt tcaaattctt gagttgtata
    21421 gaactcgaaa tatttgccat tagcataatc agacttttca aagtcttgat atttgatacc
    21481 acgctcttta gcgatttcca ttgaacgttc gattgagtag aaattcatca tcataaagaa
    21541 gatatttgca aaatcttttg cttcttctga ctcataacca attttatttt ttgctaggta
    21601 accgtgtaaa ttcatcacac caagaccaac tgaatgtaat tcactgttag cttttctaac
    21661 tcctggtgca ttttgaatat ttgctacatc actcacaaca gttaatgcgt ccataccaga
    21721 gtgaactgaa tctctgaatt ttccgctttc cattacatta acaatattta atgagcccaa
    21781 gttacaagaa atatcacgtt taatttcgtc ttcaatacca tagtcattaa taattgaagt
    21841 ttcttgtagt tggaaaattt ccgtacataa gttactcatt ttaatttgtc caatgtttga
    21901 attcggatgc actctgttag cattatcttt aaacattaaa tatggataac ctgattgtaa
    21961 ttgtgtttgc gcaattaaat tcaacatttc acgcgcattc tttttctttt tctcaacatt
    22021 tgggtttgca accatgtcat cataatattt ttctaaatcg atatcgtcta atgtcacacc
    22081 atattcttct ttaactgtat gaggtgcaaa catataaaag tccttacctt ctttagctaa
    22141 atcgaagaat ttagatggaa caattaaacc agttgatatt gtagataaac gtaaatcttc
    22201 atccgcattt acttttttag tatctaaaaa ttcttctaca tcataatgga agatatttaa
    22261 gtacacagca ccagcaccag ggcgttgacc aagttgatct gcatagctaa agccaccttc
    22321 aagtgactta gcaataggta aaacgccttt cgctacgcct ttaattcctt taattgcttc
    22381 accacgtgca cgcaatttag ataagttaat tgcaacgccg cccccaattt tacttaattg
    22441 ttttgcagtt gaatcaataa agttaattga atttaagctg tcatccactt ctaataagaa
    22501 acatgacact agctcaccac gacgcgcacg gcctgcgttt aaaaatgttg gtgtcgctgg
    22561 ttgatatctt tgttcaacca tagcagaaat aaattgttta gcttgtgctt tattaccatt
    22621 tgctaggtat aaagcaacaa tggcaacgtg ttgattatag tcttctaagt attgactttt
    22681 atcatttgtt ttcaaagcgt aatctttgaa aaatttacta gctgacatat aacttgcaaa
    22741 attaaacggg attgattttg cataatcagt gatttcaatt agatccgctt cactataaat
    22801 atcaaacaca ttgaaataaa aatcgttgtc tactaaataa cgtaaacgct cgatttcagt
    22861 gtcgaagaag attgttttgt cttttacttc ttctaaataa gctactaaag cttcttggtc
    22921 tttttctaaa ctaaagaatc catcttctct tcgtttagtg acctcattat ttaattcaat
    22981 atgattgtac tttttctctt ccatggtttt cattaaaatt acccaccttg ttcttaaatt
    23041 caataacgtc tttgtttttt ccatgtaact caaacttcat taataaaggg acattatact
    23101 cttctgatat cgtgcgaccc gcttttgcga aatttagtcc ccaatttcga ttaccgctag
    23161 ctgccacacc tctgatgtat tgatgattaa cttctaaaaa agattgaacg ggttctggta
    23221 cttctccaaa tccaatagtg ccagtaacga taataaacgg ttcatgaact ggttccatac
    23281 aattttctgc tgtaatctca agcgtatttt caagttctgt tctcttaata aaacgacgga
    23341 catttccagt aaatgaaaaa tatattattt tcattggatc accttctttc ttcaaccctt
    23401 ttaaataaag tagtcattcc aattaaaaaa tgctttaaca acatggttac tatggttatc
    23461 tcagcattgt attaactata atgaagctac ttattttctc aaatgtaaac caattgcata
    23521 gatttatatt tgagaaaata gtagcttata tgggttgaat gtataaaaat cactatatgt
    23581 agtgtttaca tcattggata tcttccattt gctcagtaca ttttctaa
//
LOCUS       DALAGF010000036        23032 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.036, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000036 DALAGF010000000
VERSION     DALAGF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23032
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.036"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            217..663
                     /locus_tag="O2692_001299"
     CDS             217..663
                     /locus_tag="O2692_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ4298934.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDKK"
     gene            879..1307
                     /locus_tag="O2692_001300"
     CDS             879..1307
                     /locus_tag="O2692_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCZ4298935.1"
                     /translation="MILNFNQFENQNFFNANPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(1414..2583)
                     /locus_tag="O2692_001301"
     CDS             complement(1414..2583)
                     /locus_tag="O2692_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HCZ4298936.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG"
     gene            2953..3591
                     /locus_tag="O2692_001302"
     CDS             2953..3591
                     /locus_tag="O2692_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HCZ4298937.1"
                     /translation="MYKINIIRSDSVYNNILKAPINDRDSIFTKEILVPFKKKFEVQH
                     MPIYNDDKQTMSAIQFLDAFQISPKDLRMSDQMSIQYLNNDFWSNCEKYLKVAIDQFS
                     NYSISSQVSNYHFTVLLGDRQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTN"
     gene            3610..3867
                     /locus_tag="O2692_001303"
     CDS             3610..3867
                     /locus_tag="O2692_001303"
                     /inference="COORDINATES: protein motif:HMM:NF021537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298938.1"
                     /translation="MKNTIYNHLHLKHIFESMPYLYGDDINKLQGRPIVGLSHAAGYA
                     CGYHLVKYFLQKTNIPIEVATTLPAQKIINEVTEFWHTHTL"
     gene            3846..4586
                     /locus_tag="O2692_001304"
     CDS             3846..4586
                     /locus_tag="O2692_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCZ4298939.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSV
                     CEFIKGLSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(4844..5497)
                     /gene="nreC"
                     /locus_tag="O2692_001305"
     CDS             complement(4844..5497)
                     /gene="nreC"
                     /locus_tag="O2692_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HCZ4298940.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(5520..6554)
                     /gene="nreB"
                     /locus_tag="O2692_001306"
     CDS             complement(5520..6554)
                     /gene="nreB"
                     /locus_tag="O2692_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HCZ4298941.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(6578..7030)
                     /gene="nreA"
                     /locus_tag="O2692_001307"
     CDS             complement(6578..7030)
                     /gene="nreA"
                     /locus_tag="O2692_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HCZ4298942.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(7050..7727)
                     /gene="narI"
                     /locus_tag="O2692_001308"
     CDS             complement(7050..7727)
                     /gene="narI"
                     /locus_tag="O2692_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCZ4298943.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKPWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(7720..8295)
                     /gene="narJ"
                     /locus_tag="O2692_001309"
     CDS             complement(7720..8295)
                     /gene="narJ"
                     /locus_tag="O2692_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCZ4298944.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(8288..9847)
                     /gene="narH"
                     /locus_tag="O2692_001310"
     CDS             complement(8288..9847)
                     /gene="narH"
                     /locus_tag="O2692_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCZ4298945.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(9837..13526)
                     /locus_tag="O2692_001311"
     CDS             complement(9837..13526)
                     /locus_tag="O2692_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCZ4298946.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(13835..14812)
                     /gene="cobA"
                     /locus_tag="O2692_001312"
     CDS             complement(13835..14812)
                     /gene="cobA"
                     /locus_tag="O2692_001312"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCZ4298947.1"
                     /translation="MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
                     VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNII
                     SKLQHYSIEAKPGICIIGEVVDYTENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            complement(14803..15117)
                     /gene="nirD"
                     /locus_tag="O2692_001313"
     CDS             complement(14803..15117)
                     /gene="nirD"
                     /locus_tag="O2692_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCZ4298948.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKGKEVGLFLTESGKIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(15121..17526)
                     /locus_tag="O2692_001314"
     CDS             complement(15121..17526)
                     /locus_tag="O2692_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ4298949.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTLDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARRIFEV
                     EKV"
     gene            complement(17592..18323)
                     /locus_tag="O2692_001315"
     CDS             complement(17592..18323)
                     /locus_tag="O2692_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HCZ4298950.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGKFTKAHEELQICAQNLQISKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHFTP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            complement(18553..19089)
                     /locus_tag="O2692_001316"
     CDS             complement(18553..19089)
                     /locus_tag="O2692_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4298951.1"
                     /translation="MTKTMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            complement(19322..20146)
                     /locus_tag="O2692_001317"
     CDS             complement(19322..20146)
                     /locus_tag="O2692_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCZ4298952.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(20363..20545)
                     /locus_tag="O2692_001318"
     CDS             complement(20363..20545)
                     /locus_tag="O2692_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298953.1"
                     /translation="MVERYIKVLILYIFTTLLSSISVTSKCVPNKVIRFILRTAIGYS
                     VFAYGLHYFSNLKKNK"
     gene            complement(20629..21096)
                     /locus_tag="O2692_001319"
     CDS             complement(20629..21096)
                     /locus_tag="O2692_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCZ4298954.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(21282..22832)
                     /gene="adcA"
                     /locus_tag="O2692_001320"
     CDS             complement(21282..22832)
                     /gene="adcA"
                     /locus_tag="O2692_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HCZ4298955.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPK
                     INQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISH
                     ESIGYLADCYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTET
                     IRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAES
                     KHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKD
                     LKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFK
                     LVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQ
                     IKEEMLAH"
BASE COUNT         7178 a         4505 c         3296 g         8053 t
ORIGIN      
        1 actactttta acaattaagg atgatacaat tacatttttt tcaatttaaa aaatcagtca
       61 tatcagtaat taccgatgaa aatactaaga tatcgatata ttttgcatta tttaacattt
      121 aaattcaatt atagacaatt caataattgc gatgaaatca gtacttgaca actattacac
      181 ttcataatat tgtttatttt aacaaggagt gattggatgt atgtagaaaa cagctatctt
      241 agcaaacagt tgtgtttttt attttatgtt tcttcaaaag aaataattaa aaaatacaca
      301 aactatctta aggaatatga tttaacctat actggttaca ttgttttaat ggcgattgaa
      361 aatgatgaaa aacttaacat caaaaaatta ggtgaacgtg tgttcttaga ttctggaaca
      421 ctgacaccat tactaaagaa attagaaaag aaagattacg ttgttcgaac acgtgaagag
      481 aaagatgaaa gaaacctaca aatttctcta acagaacaag gtaaagcaat aaaaagccct
      541 cttgctgaaa tttctgttaa ggtctttaat gaattcaaca tttcagaacg agaagcatca
      601 gatataatta ataatttgcg gaattttgtt tctaaaaatt ttgactattc cgacaaaaaa
      661 taactcccta tcataaggtg atgggcagaa gtatgcatcg atatgtttat tcattatctt
      721 tattgcttgt ataaatctcg atgctcagtt cttttgccct gattccttat caggcacaaa
      781 tctacccatt tattactttt ttatctcccc ttttacttta acttttttaa tactactcat
      841 ataatggctt tgtaatacaa atttctataa tggaagtgat gatattgaac ttcaatcaat
      901 tcgagaatca aaactttttt aacgctaatc caagtgatac atttaaagat ttaggtaaac
      961 aagtatttaa ttacttttca acaccttcat ttgtaacgaa tatatatgaa acagacgaat
     1021 tatattactt agaagctgaa ctagcaggtg taaataaaga agatattagt atcgatttca
     1081 ataataatac gctcactatt caagctacta gaagcgcaaa atacaaatct gaacaactca
     1141 ttttagatga gcgtaacttc gaatcattaa tgcgtcaatt tgattttgaa gctgttgata
     1201 agcaacatat tactgctagt tttgaaaatg ggttattaac cattaccttg cctaaaatca
     1261 aaccaagcaa tgaaactact tcatcaacat ctattccaat ttcatagtta aatttaaaat
     1321 gaatacacta ctaaagcctt aatgtcatgc tacgaatatt gcgcatgata ttaaggcttt
     1381 gtatttaact aaatgaatgg catgtattac atatcaaccg tttttcaatg agccatattc
     1441 tttttggtat aaatgacgca tggtaaataa tgcaatacat ccaaataccg ctaagaaaat
     1501 aaatgctaaa tgacttgatc ctgttaaatt agctacgtac gtgattacta gtggtgggaa
     1561 gaatcctcct aaaccaccca tcattgatac gataccattt gcggatcccg cttcatttaa
     1621 gaagtatgat ggtactaatt tgaagattaa gccgttacca atacctgcac aaatacttat
     1681 tgttaaacag cctacagtga ataatgcgat atggtctgaa atacctaaaa taatggcacc
     1741 gataatcata acaacaaaat caatcatcaa tactttaact gcattaaatt tatcacctaa
     1801 aatgccacct attggtctta agaatgttgc cagcgcaatg aatacacctg atcgaatacc
     1861 agcatctact ttgtcaattc caaaatgatt tactaagtag ttaggtaaga aaataccaaa
     1921 tgctacaaaa gcaccaaaag taataaaata ccaataactt aagtaatata atttgtagtt
     1981 tttagataat gttttcattt gagccattaa tggtacttta attttacgtt cttgtgtgtc
     2041 accaaaaatg aacataatta atgcaaataa agcgataata attaagtaac ttctaactgt
     2101 tgtttgccaa ccaataatac ccgctatcgg tggtgctaaa aatgaagaaa ctgctgtacc
     2161 gatatttccc ataccataaa taccatttgc tagacctact ttttctttag ggaaatattt
     2221 aggaactgat gtaacaccaa ctgagaaaat tgcaccacct acaccaagga aaaatcctga
     2281 agccattaac atacccggtg tttgtgcttg gcttaagaaa aatatcggga ataacaatac
     2341 gataaaacta gtaaagaata cccatttagc gccaacaatg tttgttaaat aaccaaatgg
     2401 cacacggagc accgatccca aaataactgg tatcgctaaa atgattgata tttgaccttc
     2461 agtaacattg acatcttgtt taataaaggg cattaatggc gcaattatac tccaagccat
     2521 aaacccaacc actaaactta atgtttgtaa tgtaagttga aagccacctt ttgttttgta
     2581 catacgtttc accttcatca aaatcattta ttttttaata gatatcaata ctctgcctat
     2641 atattagagg tcaatgttat ttaaaaatgc tttcatttat atgaaaacat tcaatataga
     2701 caaagtattg actacatata tattttatta tgattgtgaa aaagtgaaca taaggatttt
     2761 cccttattta tgaagtgatt taccctatac aatgtccctt taagatgctt ttagacactt
     2821 attcgccatc attttaatca tattatttag gaacactcac atacttttct taattttttt
     2881 caattagtta caattactgc ttgaaaatca cgttacgtta tcctttatgc tgacaacata
     2941 aggaggttta tcatgtataa aatcaatatt attagatcag attccgtcta taataatata
     3001 ctcaaggcac caataaacga ccgtgattca attttcacta aagaaatatt agtacctttt
     3061 aaaaagaaat ttgaggtaca acatatgcct atttataatg atgacaaaca gacaatgagt
     3121 gcgattcaat ttttagatgc ctttcaaatt tctcccaaag atttacgcat gtctgaccaa
     3181 atgtcaatac aatacctgaa taatgatttc tggagtaatt gcgaaaaata tttaaaagtt
     3241 gctatagatc aattttcaaa ttatagtatc tcatctcaag tttctaacta tcatttcaca
     3301 gtattacttg gagatcgcca aaaaccactt atgtatctaa ataaaaatcg cggtggtgat
     3361 ggtggcatac caggttatat tatgatttat ttagtgccga gtacaagtac aattaattct
     3421 atgaaaagct taattgcaca tgaagtaaat cataatatgc gctatcaata tattgattgg
     3481 gatggcggaa gtttgattga attgattatt gcagaaggat tagctgaaaa ctatgtagaa
     3541 tcattgtatg gtaaagcgca tattggaccg tgggtaacaa atactaattg aagtcgtgat
     3601 aacgtaaaaa ttaaaaatac tatttataat catttacatt taaagcatat atttgaatcg
     3661 atgccttatc tctatggtga tgatattaat aaacttcaag gtaggcctat cgttggctta
     3721 tctcatgctg ccgggtatgc atgtggctat cacttggtaa aatacttttt acaaaaaaca
     3781 aacataccta ttgaagttgc tacaacactt ccagcacaaa aaataataaa tgaggtgact
     3841 gaattttggc atacacatac actttaaaag atattattga aattacaggt gtaactaaaa
     3901 gaactttaca ttattacgat gaaataggat tattagttcc agataaaaat gataaaaatt
     3961 atcgcgttta taaacagcaa gacttagaaa aattacaaaa gattttaata ctcaagtctt
     4021 ttgattttga tatcgctaaa ataaaacaat acatttcgta tgataatgaa caattgcgaa
     4081 agttattatc agagcaaata agcaagttag ataaaaagat ttctgactta caattaatta
     4141 gacgctctgt atgtgaattt attaaaggac tctctctaat agataccagc attttaaaca
     4201 agacactaca gtcacaatat gataaagaag catctataaa atatggtcat acgaaagcat
     4261 atcaatcatt tattagacgt aaagacagct tacaatcgca ggatatcaga cataaattga
     4321 caactatctt caataaattt aatcatatgt ctttgagtca ttatccaatc caagattgta
     4381 gtgatctcgt atttgagtgg aaggcattta tgaacactat cgctgatttt gatgatgaaa
     4441 cattatgctg tattgctaaa acatatgaag atgatacgcg tttcaaagat tactttaatt
     4501 catatgataa tcaaaattta gcatcataca tttcagaagc tgttaattat tttttgagca
     4561 atgtgaataa gagcgacaat ttttaatcct catacagatg ctacaacact gaatacacca
     4621 actaaaataa ttgttcaaag tagaaaacta atcatttatt ctctaaatca ataactatat
     4681 taaaagttat acctttgcaa agcgaattag tataggttac cgaaaggaga aaggattagg
     4741 ttccattcga tttataaaaa aatatatttc gaaacataca aaagcgccag aagataatca
     4801 tttcctctcg cgcttttata ctgaattata ttaatttcaa actctaaaac tctaataatt
     4861 tctttttcaa tgcatattca actagctcag gtttactctt taagccaagc tttgtcataa
     4921 tatgcgtctt atgtgcttct actgttttca cagatacaaa taatttctct gcaatttctt
     4981 tattcccgta acctttggca ataagtggca atatttctag ttctcgtttt gataagattt
     5041 taaaaggatc tgttgtgttt gcagtgtcct gatttgaatt agatacaaat tcattcacta
     5101 aagatgttgt aagtttcata tctacatatg tttcaccttt atatacagtt cgaatagcta
     5161 acaataattg ttcatcaggt gcatttttca atatgtaacc tttcgcacca ttacgcaaca
     5221 catggaacaa atactcctca tcatcaaaca ttgttaatat tagtatttta gtttcaggaa
     5281 aactgtcagc aattttactc gtagcgataa gacctgactc acctggtggc atacttaaat
     5341 ccattagtaa cacatcaggt ttatattcca ttactttttg gtaagcttcg acgccatctg
     5401 cagccgttgc aacaacttcc atatcatttt gataatttaa aatcatagag aaccccgtac
     5461 ggacaacagc gtgatcatcg gcaatgacta ttttcaattt tattccccca atgtatgttt
     5521 caaattggaa tgttcaatgt aacattggta ccctcaccaa ttttcgtttc aatattgacg
     5581 ctaccgctga ctaactcagc tcgctcattc attccatata aaccgagtcc agaaccttta
     5641 ggcttagaac ttggatcaaa accatttccc gcatctatca cttctgctac caaatggcgc
     5701 ccagtttgac ggatacctac atttatttca tttacatcag cgtatttcaa cgcatttaaa
     5761 atagcttctt gcactactcg ataaacaacc gtttcaatat cactatcaaa gcgagtattt
     5821 ttaatatttg atgtatatat gatttttatt ccataatttt cttcaaactg tttaaaatat
     5881 gatttaaaag ctgcttcaag gcctagatca tccaaagaag cgggtcttaa ttcaaccgac
     5941 atattacgta tatcatcaat taatttagcg acaatatatt caatattttc tgcgtcttcc
     6001 aaaagcttag ttgtatcttc ttgatatttt aataatctca attgaacatc tacattgagc
     6061 atttcttgaa tcacactatc atgtaactct ctagaaattc gctttctttc attttcttgg
     6121 gctgagattg ttttacgcat catacgttgt tgatgcaatt tctcttgctg ttcaatttgt
     6181 gatgaaacat tttgaagcgt aaatgcatga attcccctgt cttgatcaat caactgatat
     6241 gttgctgtaa atggcatcac tttttgatct ttcgtcttca taaatacttg gaaattcgta
     6301 gcttgtactt gcatcgattc taagaaacag tctttacatg attgtacatc ataggcattt
     6361 gtataccctt cacatctgtg acaaatcgca ttcgccacag cactgtaatt atcttcttca
     6421 gataatatat ctttagcagc atcattcatt gcaataattt taccgttatc atcagcaaaa
     6481 actatctttt cgattgaatg ctcataatat tttttcaata aagtatctaa ctgtatactg
     6541 tcctcattaa tcatgactta caccctaatt catctcatta tttatcatca ttgaaaatac
     6601 caaacttacg ttgaatatca tcattatcaa atatttttgg taaaggacga ccatctcttt
     6661 gaccaaataa tagtacgcca tacacttgat tcttatacca aagcggcact gctaaaactg
     6721 ctgttaatga ttcgctcaat aaaattggat agtcaatctt ttcttcaggc cctaaagcta
     6781 aaccaacatt ggctattacc atacgctttc ctgttttcat aacagttcca gctaatccac
     6841 gaccttttct taaaataatc aatttaaatc gattattttt attacctgaa acatagtgcc
     6901 attttattgg agatgatggt ttgttagatt catagaaagc gattgccgca aaatcataac
     6961 cctcttcttt gcgtatttta tctaatgtct cttgaaatct acgatcttca attattgctt
     7021 ctggtgtcaa atcctttcac ctcttatgct tacactttat tcttacggta aataatatat
     7081 ctgcgattta tatatgtcaa aggtacactc caaacatgca ccaaacgtgt aaatggccaa
     7141 caagccataa tagtgaaacc taacaatata tgcattttaa atgcaatcgg cacaccactc
     7201 atcaatgacg catctggttt taacataaat aattgtctaa accaaattga taatgaagtt
     7261 ctgtagttaa agtctggatg ttgtatattt gttactaatg ttgcgtaaca tcccataaat
     7321 acgataagta ataataagaa atttacaaat atatccgacg ctgaacttaa tcttcgaata
     7381 cttttcgtag taacacgtct cgctgttaat aaaaacatcc ctatcaaagt tattatacca
     7441 aagatgctac caatataaac agcgcctata tgatataaat gctcagacac acccactgca
     7501 tccatccatg gtttcggtat taacaatcca actacgtgtc caaaaaacac tggaataata
     7561 cctaagtgaa ataataaact tccccacatc aacctttttc tttctattaa ttcactagat
     7621 ttagctgtcc aagaaaattt atcataacga taacgtgcaa tatgacctgc gacaaagaca
     7681 actaaacata aatacggaaa tataacccat aaaaactgat taagcatgat gtttcactcc
     7741 ttttggtgat gtcaaacata atttcaatgt ttttctaagt gcttgaatca cataggcata
     7801 tggattgtta tcttcaccaa gtgcattcgc catcacatat gttccatcct caataatcat
     7861 aatgattaat tgaatattct cttcagctct tggatcattt cgccattctg ccacttgcaa
     7921 aaattgaagc atcaacggta gataatcaga aagttcatta tctaccattt ctagtccaaa
     7981 catttcatat aataccttta atttagctaa catttgccca cgttcttttt gcgtatcaaa
     8041 tttgttatac gtcatatata atggtgcttt tttcgtaaaa tcaaatgtat ctgtataaat
     8101 cgctttgatt tctgataatg aaaatgtcat cattacattt ctaaatgcta ctaaatcttc
     8161 atatgctgga tgatctttcg aaattgtttc ttcaaaagtt tttggatgaa aagttaattt
     8221 ttctggaaaa cataactgtt gtgccatata tccaaaactt tcttgatatt ttttaaaatt
     8281 atcgaaatta atcacggaaa atccctccat agaaattctc attataaatt tcttgaccag
     8341 ttttccctga acctactgca acgccacagc cttcacagtt atctccaaaa tgctcgccgc
     8401 cgtaattgta tcctgtacta ccttgtgcgt gatacgtatc taaataggtt tctttgtgtg
     8461 atgttggaat aacaaatcga tcttcatatt tggctagtcc taataaacga tacatgtctt
     8521 tagtttggcg ctcggttata cctaatcgct ctaatcgaga cgtgtcaaat ggctgttgag
     8581 taacttgaga tctcatataa cttctcatca ttgccatacg ttgtagggct ccttttactg
     8641 gctctgtatc tcctgcagtg aaaatattag ctaagtattc aataggtaaa cgcatttctt
     8701 caatggctgg gaaaatcgca tctggatttt gagttgtatt tttaccttca aaatagctca
     8761 taattgggct aagtggtggg caataccaaa ccatcggcat cgttctaaat tcaggatgta
     8821 acggaaatgc aagtttatat tcaattgcta acttataaat tggagagttt tgtgcagctt
     8881 caatccaatc gtaaccaata ccatcttttt cagcttgagc aatgacttct tcgtcaaatg
     8941 ggtttaagaa tatatctaat tgtttttcat ataaatcttt ctcgtctact gctgaagctg
     9001 cttcatgaac tcgatctgca tcatataata aaacacctaa gtaacgcata cgtcctgtac
     9061 aagtttcaga gcataccgta ggcatacccg cctcgattct cgggaaacag aaagtacact
     9121 tttcagcttt gttcgttttc caattgaagt aaactttctt atatggacaa cctgtcatac
     9181 agtaacgcca tccacgacat gcgtcttggt caactaatac aatgccatct tcatcacgtt
     9241 tatacatagc acctgaagga cacgatgcaa cgcaacttgg attcaagcaa tgttcacata
     9301 aacgtggtaa atacatcata aaagtttcgt caaattggaa tttaatatct tcttctattt
     9361 tttggatgtt aggatctttt ggacctgtaa catgaccacc tgctaagtca tcttcccagt
     9421 taggtcccca ttcaatttca atgttatccc ccgtaatttc tgaatacgct ctagcaactg
     9481 gcgaatgctt ccctgatttc gcagttgtta aatgttcata attatagttc catggctcat
     9541 aataatcttt aattaatggc atatctgggt tataaaaaat tttacctaaa gcaatttttg
     9601 aaattctact tccagatttt aattcaagtt tccctttacg atttagtacc caaccacctt
     9661 tgtagtgttc ttggtcttcc caacgtttcg gataccctac acctggcttc gtttctacgt
     9721 tgttgaacca catgtactca gcacctggac gatttgtcca agtgttttta catgtcacac
     9781 tacacgtatg gcatcctatg catttatcta aatttaatac catcgcaact tgcgctttaa
     9841 tcttcaagcc aattaacctc cttcatcttt ctaactgcta catataaatc cctttggttc
     9901 ccaattggtc cataataatt aaagtgataa ctaatttgtg cgtatcctcc gactagttgt
     9961 gttggtttca aatggattct agtcggcgcg ttgtgtgaac caccacgtgt atctgtaatt
    10021 tctgacccag gcgtttgaat atgtttatct tgtgcatgat acataaacat tgtaccttta
    10081 ggcatacgat gcgaaataac tgctcttgcc gttacaacac cattacggtt atacacttct
    10141 agccaatcat tatcttggat atcgtgtttt tcagcatctt catttgatat ccaaaccgtt
    10201 ggaccacctc taaatagtgt caacatatgc ttattatctt gatacattga gtgtatattc
    10261 cattttccat gaggcgttaa ataacgcagt accaaagcat ctgtaccacc tttaattttc
    10321 ttatctctat tcccaaatac cattggcggc aatgtcggtt tatatactgg taagctctcc
    10381 ccaaattgtt ggaaaacttc gtgatccaca taataacttt gacgtcctgt taatgttcta
    10441 aaaggtacta gacgttctat attcgttgta aatggtgaat atcgtcgacc ttgtttattt
    10501 gaacctggga atactgctgt cggtattact tctcgtggtt gtgaagttat atttaaaaac
    10561 gaaattttct cagcagcgcg ttcgctagaa atatctttta acggcattcc agtttgttct
    10621 tcgagatctt catatgattt ttgtgataat ttaccattcg tagcagatga aatacttagt
    10681 attgcatcag ctacattacg tgctgtatca atacgtggac gattcgctct cacagaatca
    10741 tcatttgtat cactccacgt acctaacata ctttttaatt cttcatattg ttcactgaca
    10801 ccgaaactta caccatgtgc tccaactttc cctttttcaa gtacaggacc aagcgtgaca
    10861 tatttgtcgt aaattttagt gtagtcgcgt tctacaattg caaagttagg cattgtacgt
    10921 ccaggtaccg cttcaatttc acccttcgac caatctttca ctacgccgta tggtgttgaa
    10981 atttcttgct ttgtatcatg actaagtgga gttgtcacaa catctttaaa cgttccaggt
    11041 aaatagtctt ttgccatttc tgaaaatgct tttgccaacg ttttataaat atcccagtct
    11101 gaacgcgatt cccataacgg atcaatggca ggattgaaag gatgtacata tggatgcata
    11161 tccgttgatg ataaatcatg tttttcatac caagtcgctg ccggcaaaac aatgtcagaa
    11221 tataacggtg ttgccgtcat tctgaagtct aaagagacca ctaaatctaa cttacctgtt
    11281 gtttcttcac gccacgtaat ttcttctggc ttttcatctt catttggtgt agctaataac
    11341 cctgattttg tgccaagtaa atgcttcata aagtattctt gaccttttgc agaacttgaa
    11401 attaagtttg aacgccatat aaataatgat tttggatgat tctttttcaa atcaggatct
    11461 tctattgcaa attgtgtttg ttttgatttc acttcatcaa ttgcacgttg caaaatcgct
    11521 tcatttgaat ctataccttc atctttagct tcttctgcaa acaacaaact atttttatta
    11581 aattgtggat atgatggtaa ccaaccaagt ctagctgcta aaacattata atcagctgga
    11641 tgttgatgct ttaactcctc tgttttagct aatggagatt ttaaacgatc tacatttgac
    11701 tcttcatatt tccattggtc tgttgcaaaa tagaaccaac ttgttccgtt ttgcaaacgt
    11761 ggtggtcctt gccagtcttt cgcaaatgcg acagtactcc atccttcaat cggacgacat
    11821 ttttcttgtc ccacatagtg agcccaaccg ccaccgttca caccttgaca gccacataac
    11881 ataactaagt ttaagattga acgataaatc gtatctgagt taaaccaatg gttaataccc
    11941 gcacccatga taatcattga acgcccttca gtatcgatag cgttttgcgc aaattctttc
    12001 gctacttgaa tgacaacact ttgttttacg cctgaaatgg cttcttgcca agcaggtgta
    12061 tattttgatt ctgcatcgtc gtatcctttt gattctaatt tatgatcaaa acgacgcacg
    12121 ccatattgac ttgccattaa gtcaaaaatt gtagcaatac ggactttgtc accatttgct
    12181 aaagtgactt gtcgagttgg aattggacga ttgaatatcc catctccatc actatcaaag
    12241 tatgggaatt gaattgtttc taattcgtat ccaccttctg tcattgataa tgtagggtta
    12301 attttagaac catcttctgt ttctagtttt aagttccact tcttaccttc ttcccaacgt
    12361 tgacccattg tgccattagg tactactaaa ctatcgctga ttgcatcatg aataactggc
    12421 ttccattcgc cttgctctgt tgtttgacct aagtcactcg ctcttaaaaa tcgacccgct
    12481 ttatatccat tttcatcttc atccagcatg ataagaaacg gcatatctgt atattgttta
    12541 gcgtaattta taaagcgttc attaggttga ttaacataat gttcttgtaa aataacatgc
    12601 gtcattgctt gtgcaattgc agcatctgaa ccaggattcg gtgctagcca gttatctgca
    12661 aatttcacat tttctgcgta atctggtgct actgaaatga cttttgtacc tttatagcgg
    12721 acttcagtca taaaatgtgc atccggagta cgtgttaaag gtacattaga gccccacata
    12781 ataatgtatg atgcgttata ccagtcactt gattcaggca catctgtttg ctctccccaa
    12841 atttgtggag aggcaggtgg taaatctgca taccagtcat aaaaactaag catttcacca
    12901 ccaagcaaat tgatgaatcg agcacctgct gcataactaa tcattgacat cgctggaata
    12961 ggtgtaaatc ctgcgattcg atctggacca tattttttta ttgtatacag taattgtgct
    13021 gcgattatct ctgtaacgtc tttccaattt gaacgcacgt gccctcccat acctcgggct
    13081 tgcttatatt gtttggcttt gtcttcattt tcaacaatag acgcccatgc agcaacgcga
    13141 ttaccattgt tttcttctaa tgcttcagtc cataaatccc agagttttcc acgaatatat
    13201 ggatatttga ttcgaagcgg actgtattca taccaagaga atgacgcacc tcgtggacat
    13261 cctctcggtt catattcagg catatccgga ccacaacttg gatagtcagt ttgttgattt
    13321 tcccaggtaa tcacaccatt tttcacaaat actttccaag aacatgagcc tgtacagtta
    13381 acaccatgtg ttgttcttac ttctttatcg tggctccaac gttctctgta cattttttcc
    13441 cattctctac ttttactttc taggatcgac caattcccat taaatttttc tgttggctta
    13501 aagaaattca atccaaattt tcccatattt atatcctcct acgtataaaa atacgatgtg
    13561 tagatgtcgt gtttttaaat actttaaaat gcccaagact attgctttaa ttagattgta
    13621 cattttttca caaacataaa atattaggga atcacctaat tacttaagga atttccctat
    13681 caataacggg atttcattga aataatacac aatcatgtat ggtcatgctt attgccaatc
    13741 taaatcgttc aaatttggca caacgacaaa taaggcttca acacgaatat attctctcgg
    13801 ttgaaacctt acttattcat ttatttttta taaattagtg acataacact gtattagcat
    13861 ctgcacgatc ggttgaaata tatgttacat tttcttgctg cttaataaat gcatcatagt
    13921 aatcatattg cgacgaatga tatgtgccat tcgatgtatc atttgggttt agcaaacagc
    13981 cataaccttc gtcatataaa tgttcacaga gcataagggc gtcatgttta gaaccactta
    14041 ctacataaaa ttgcttcata ggatcatatg atttaggagt gttttcagta taatcaacaa
    14101 cttcccctat aatacatata cctggtttcg cctcaattga atagtgttgc aattttgaaa
    14161 taatattact taaacgcccc ttaacaacaa actcgttaaa acacgatgct tgaaagacaa
    14221 tcgctatcgg gtaatcaata tctgtgtatt gttgtatctg tgtgataatt ttccctaaac
    14281 gttttacccc catataaatt gctaacgtgc caccattcac taaggaattg acatccactt
    14341 cattttcttc tgaatcttta aagtgacctg tagaaaatgt cacactttta gcaactgtac
    14401 gcattgtcaa acctgtctgc atagtagcaa ctgctgcgct cgctgatgtc acccctggta
    14461 caatttcaaa cgcaatatga tgttcattta gtgtgtcgac ttcttcttgc acacgaccaa
    14521 atatcgctgg atcgccacct ttaagtctaa caaccttgtt atatcgacgc gctgcttcca
    14581 cgatacagtc atttattttt tcttgctgaa tatgttttgc atacggcttt ttaccaacat
    14641 cgataatttc agtagtcaaa ttcgcatatt gtaaaattaa cggattcact aatcgatcat
    14701 atagaatgac atccgcttca cgtattaaac gctcagcctt tttcgtcaaa taattcggat
    14761 tacctggacc cgcacctatc aagtaaacct tgccatattc ctctacagac atatatatac
    14821 gttcccgtct gtaacttcta cctcataaac atctacacaa ccttcatcag gttcttgaac
    14881 aatacctgta tttaaatcaa ttttttgatc gtggagcggg caaaatacat attccccact
    14941 cactgtccct tcagacaatg gtccttgttt gtgtggacag atattgtgaa tcgcatgaat
    15001 tttgccactt tctgttaaaa acaaccctac ctctttgcct ttgacaataa ccttttttcc
    15061 aattaggggt gttaattcat ctatagttgt cactttaatt ttttcttttg tttccatgta
    15121 ttacaccttc tccacttcaa aaattctacg tgcttgcgca ttgctagtta ttgcttccca
    15181 aggttcagct tcgactgctt ttttagcatc cataatgcgt tcaaatagtt cattttgtct
    15241 ttctgggtca agtaagactt cttttacatt ttcaaatcca agtcttctta accatggcgc
    15301 tgttctttca gcatatatac ctgtttcgcg ataatattgc atcaaagcac cacataattt
    15361 gattacttca tcttctgttt ctacagttgt taaaaattca gctttttcaa cttctgtacc
    15421 accattacca ccgatataga tttggaatcc attttcaact gagataatac caaaatcttt
    15481 aacacctgat tcaacacaac ttcttgggca gcctgataca cccattttga atttatgagg
    15541 tgtatcgatg tattcaaatg ttttttctaa acgaatgcca agtcgtgtcg tgtattgcgt
    15601 accaaatcga caaaactctt taccaacaca gcttttaact gagcgtgttt tcttaccata
    15661 agctgatgct gaacgcatac ctaggtcttc ccatatattt ggtaattctt cttttttaac
    15721 tccatacaaa ccaacacgtt gtgaacctgt cactttaact agtggcacat gatatttctt
    15781 agccacttct cctagacgaa tcagttggtc tgcatctgta acacccccac gcatttgagg
    15841 tataacagaa aatgtaccat cattttgaat attcgcatgg taacgttcgt tagcaaatct
    15901 tgattctctt tcatcttcat gatcatgtgg ataaaccatg tttaaataat agttgattgc
    15961 tggtcgacat tttggacatc cacctttatt tttaaagttt aaaacatgtc gaacttcttt
    16021 agatgttttt aaacctttcg ctcttatttg cgttactatt tgatcgcgtg tcaaatcagt
    16081 acaaccacat ataccagcag gttttgcggc aacaaagtca tctcctaagg tgtgctgcaa
    16141 tatttgagca atttgcggtt tacatttacc acatgaattc cccgcttttg ttttagccgt
    16201 tacttcttca actgttgtaa agccattttc cgtaatcgca tttactatag tacctttatc
    16261 aacaccatta caaccacaaa ttgtttcatc atcagccata tcagcaattg atagcgatgc
    16321 ctcttctcca cctttagtaa gcaatgatac aagtgtgtaa tcttcagtgg attcaccttt
    16381 tttcatcatg ttataaaagc gtgaaccatc atcgatatca ccatatagta ctgcaccaac
    16441 tacattaccg tcttttaaaa agattttttt atagttatta tcaacactat taaatatttc
    16501 aataccttta atttctgcat tttctacaat ttgaccagca ctatacaagt cacacccaga
    16561 aacttttaat gacgtaaatg ttgttgatcc cttgtatccg ttcgtttctt tatttgttaa
    16621 atgatcagct aatactttac cttgttcata tagtggtgca acgagtccat aaactttgcc
    16681 gttatgttct gcacattcac caactgcata tacattgcta tcacttgttt gcatcacatc
    16741 attgacaaca ataccacgat taacatctag acctgattct ttggctactt ctgtgtatgg
    16801 tcgtatacct actgccataa caactaagtc tgccggaatc tcgcgtccat cagccaattt
    16861 aacaccctca acatcatctt ctcctaagat ttcagttgtg ttggcttgca tttcaaactt
    16921 cataccttgc ttttctagat ctgctttaag catatttcca gctttacggt ctagttgcat
    16981 ttccatcaac cattcagcta aatgtaacac cgttacttcc ataccttgat ctaataaacc
    17041 acgtgcacac tctaaaccta gtaatcctcc accaattaca attgctttct ttttagtctt
    17101 agcaatgttc atcatttgtt cagtatcatc aagcgttctc caaccaatta cactcggtaa
    17161 tgtcgaacct ggaactggaa tgacgaatgc tttagaacct gtcgcaaaaa tcaatttatc
    17221 gtatgatact tcaataccat ttgcagtagt aactgattga tttgctctat ctacttcaat
    17281 tacaggatca tttgtaatta actcgatacc atgttcctca taccactcat atggattcat
    17341 aattgtttct tcaactgtca ttttattttg taaaatattt gaaagcatga tgcggttata
    17401 gtttggataa ggttctttac ctattaccgt aatatcatat aaatcgttgg cgcgctctaa
    17461 tatttcttcg attgttcgaa tgcccgccat accgttacca atcattacta gtttttgctt
    17521 tgccataaaa tatgcccctt tactccataa tatttatttc aaaaaaaggt attaattttt
    17581 cgttagtgct tttatatttt cattggaatc attaagcttt ctaatctatc gttaatgatt
    17641 tgctttaaaa ttgggtcgaa gttaattgaa ggtgtgaagt gtatatctgt attaataacc
    17701 atgtcattca tttgctgctt cactttgtta acaagtcttc cgtcatataa aaataatggt
    17761 acgacaatca atttttgata ccgtttcgag atgctttcta aatcatgtgt aaaactaatc
    17821 tctccatata gcgttctcgc atatgtcggc ttgctaattt gcaaattttg agcgcatatt
    17881 tgtaactctt cgtgtgcctt agtaaacttt ccattaatat tgccgtgtgc aacaaccata
    17941 actccaactt gttgttcgtc acctgctaat gcgtcacaaa tacgttgttc aattaatcgt
    18001 ctcattaaag gatgtgtgcc aagtggctcg cttacttcta cctttatgtc tggataccgt
    18061 cgtttcattt catgaacgat attcggtata tccttgagat aatgcattgc actaaagatt
    18121 agcaatggta caattttaaa atggtcaacc ccactttgaa tcaacgtcgt cattaccgtc
    18181 tctaaatcct gatgctcact ttctaaaaac gcaatatcat agtgatgtat atcatctttt
    18241 actaattcag aaataaatgc ttctaacgct tgattctgtc gtccgtgcct catgccatgt
    18301 gcaacaatga tattcccatt cacatttacc aaccctttca cacgtattgt ataccaaatc
    18361 attttgtttt tgtgaaaaga atcacattat aatgtaaaat cagggaattc cctgatgcct
    18421 gtagtcatgc atattcctta tacattttcc ctttttgtta aatcaaaaaa agcgaccgat
    18481 atatgaatcc ctactcaaca tttatttgag caagcattaa tatatcggtc gcttgtagtg
    18541 tatattatta tcttaaaatg gtggttggcc taatattgtt tcgtcaaagc gctcgggtat
    18601 caatactttg cgcatgatca cacctaaatc gccatcatca ttttcatgtt cgctgtatat
    18661 ttcataacct cttttttcat aaattttaag taaccacgga tgcaatcttg cagatgtacc
    18721 taaagtaact gccgctgact ttaacgtatc tcgcaaaaat gcttcttcaa cataagtaag
    18781 taattggcta ccatagcctt tcccttcata ctcaggattt gtcgcaaacc accagacaaa
    18841 aggataaccc gaaatacttt tcacacttcc ccaaggatat ctaaccgtaa tcgtagatat
    18901 aatttcatca tcaattgtca tgacaaatgt agtattttta tctatatttt ctttaacagc
    18961 atctaaatta gcattaaccg aaggccaatc aatacctagt tctcttagag gcgtaaatgc
    19021 ttcatgcatg agttgttgca atttttctgc atcttgttca cttgcgagtc gaatcatcgt
    19081 ttttgtcata ttaatcccca ctctttttta aatgatttaa ccatatttta tttttaaaat
    19141 aaatatccat caaagtgtat caataaattt atcacatgtc agaaagtatg cttcatctga
    19201 atacaccaat actctcatga aacttattaa aaattactct ctcaacgtaa aaaaccattc
    19261 aaattcatga atggtttgga agaatgattc attgttacgc tatttaatca ctacatctta
    19321 attattgttg ctctaaacga ttacgcttac catttaagaa agcataaacg agacctacaa
    19381 aaataccgcc accgacaaag ttacctaaga aagcaaaaac gatatttttt aaaacgtgta
    19441 accatgaaac tgcatcaagg ttaaagaata ccatacctgc atatagacct gcattgaaca
    19501 caacgtgctc atatcccatg tatacaaaga ccacgacacc acaagctatg aagaatgcct
    19561 ttgttaagcc gcctttgaat tgcatagaga tgaaaatacc aatattaata aagaagttac
    19621 agaaaatacc ttttgtaaaa atattcaacc atgttgaatc aacagtcttt ttctgaacta
    19681 aagctgttaa agcttgtgtc atttcaggtg tcatgacatg cgcaaatttc attaagaaaa
    19741 ataacacaaa cccacctaaa atatttccta agaaacaata taataaaatc caagtcatct
    19801 tcttaacaga aacgacttta taataccagc ctactgtaaa gtacatgaag ttacttgtta
    19861 ataattcaga gttagttaat acaactaaaa tcaaacctaa actaaacgca atggctccca
    19921 ttaaattgat aagtccatct acgtgagtac tcgcaaactg cgtctttatt ccgaacataa
    19981 atacagtaac gattgagagt aaaaatccgg ccatcattgc tttaagcata tatcggccag
    20041 gtgtttgctg taccattact tgcttcattt gtactgaaga tgatattgaa tcaacaattg
    20101 cgcctgttga atatgtatct tcaactgttt tcttattttc tatcacagta cttcccccat
    20161 ttactttgat caaataaaat ttactttcta attatattag taaatcaatg taattgatag
    20221 attaaatttc agaaaaatct ttttattctc tcgaaaatgt agttttcttt cataaaaaca
    20281 gcgagataaa tcgcgtgatt ggctaatatc tttatgattt aagatattac gacgctcatt
    20341 ttatctcgct ttatgtaaag tattacttat tcttttttaa atttgaaaag taatgtaaac
    20401 catatgcaaa tacactgtaa ccaattgcag tacgtaaaat aaaccttata actttattag
    20461 gtacacattt tgacgttact gaaatagaag atagtagtgt tgtgaaaata tataatatta
    20521 aaactttgat ataacgttct accatcgaat catccctcat ttttataata ttacaagatt
    20581 attataaacc gaatgaaaca aaaaagtttt tacatatttt caacaacttt atttcaataa
    20641 agcatcaagt tgatctaaag tttgattcat accttgttca acacccatgt ttatgacttg
    20701 ttgagcggct tcttttgttg gaaataccga tgttgatgtc actgtcgttt tcgttgtatt
    20761 gctcttggaa aatgacaaag taattttcat gcttggcatt tttgtatctt ttgcaccttg
    20821 aggtgtcgca aatgagtcaa tatattcaat taaatatgga cgcttaaccg ttttatattc
    20881 tgctaatgta tagctggtca ttgtaggtgt tttaatcgca taaaatgcat caccgcctga
    20941 aacagcatta aagcgaaaca ctttggtact agcgtctttt ggatgaaacc atttttcaaa
    21001 taatgctttt gtcgtatagg catcaaacac cttttcaatc ggtgcttcta tcgttcttga
    21061 aaaaataatt ttattatctt caactttaat cgtcatcttc ccactcccta tcttttatat
    21121 ttactcatct taacatgcat tgagtaacat atgttaccaa atcatttgtt ataaactata
    21181 gtaatattaa tcattccctc tgttgaaatt gataactacg aaataaaaaa cacactctat
    21241 tcagctatta acagttgagt agagtgtgca aacctgttga attaatgcgc taacatttct
    21301 tctttgattt ggtctttatt taattttgaa ggataatatg ttggccagtt atccatttct
    21361 tttaataacg cgtcattatc attacccata aagatatgga agtgttctgc ctttttaggt
    21421 gcaatgttgt gatcgctaaa ttggatgaat ttcggtaagt ctttatcatt accatcgacc
    21481 aatttaaaca taaatctcac gccacggtta cctttaggat atttcaatgt tttcttacca
    21541 ttgtattcat atttaccagt gtgtttctta ccatctttag taaatgtaat ttcatttcct
    21601 tttatatcaa tgttagtaat atcagtttta tatcctttgt cataataagc ttttaaatct
    21661 ttagcagatt tcttcggatc attttcagct ttatgttcca tcacttcatc aagcgtaccg
    21721 tcttttaagt aagggtaaac agattgccat tcaccagcat aatcgcttaa ttcacggtct
    21781 ttaacttgct catctttaaa atacccatca gaaattgctt tgtcatgttt actttcagct
    21841 ttgtcgtctt tcactttaac accactgtct aaagctttac cgatattttc aatattagat
    21901 ttcattaatg attgatacgt aatattatct tttttctgtt gttctttatt taaagactcc
    21961 atgttgtaga attttaaagg cttcgcatct gtttctttac gaattgtttc agtcacttta
    22021 ttcgcaacat tatcttcgta aagaatatat ttagcattgc tatctctaat ttctttaaca
    22081 attttagtta attctttttg tgatggatct tcagcattca tgttttgaat acctttttga
    22141 acaaaaccat aacaatcagc taagtatcca attgattcat gtgaaatgaa tactgcatta
    22201 ccttgcttat cttttgtaac ttgcttcata tcgttatcaa ttttcttaag atcgtcgttt
    22261 aattttttgt agtttttctc atagtcatct ttatgttttg gatctttttt cactaattca
    22321 tctttaattt ctttagcgaa agtttggtta attttaggat ctaaccatac gtgtggatca
    22381 tatccaccat gatgatgatg atgttcttct ttctcatgat catgtccctc atgttcatgc
    22441 tcttcaccat gctcgtgttg atcagttaaa agctttgctt tatctaattt atcctctaaa
    22501 gacagttttt tatctttatc tttgatagta gatgcaactt tcttagcaac cggatctaaa
    22561 ttatcccctg tatacataaa caagtctgac ttgcttgcac ttaatatatc tttttgtgtt
    22621 ggttcatagc tatgtaaatc tgtccctgct ggatagattg atgatacctt cacgtgtttt
    22681 ccaccaattt gctctgcaaa tgattgcaat ggataaactg tcgttttaat tgttaccttg
    22741 ccatctttat cttttccatc atcattccca catgcggcta atgataaagt tacggctggt
    22801 acaagaagta acatacctaa tttctttttc atagtcaccc tcctaaatag taatctttac
    22861 gttttaaaat tatatactca tgctacttaa aatacaagca ctgtttttat aaataatata
    22921 aagtcgaatc atactttagg tatttaccac ttttcgtttt atctaataca tgactattgt
    22981 acaaatatga tacagaaaac tttatagtat ttacgaacgt cataatataa ga
//
LOCUS       DALAGF010000037        22525 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.037, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000037 DALAGF010000000
VERSION     DALAGF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22525
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.037"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            1..1860
                     /locus_tag="O2692_001321"
     CDS             1..1860
                     /locus_tag="O2692_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HCZ4298956.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDIDKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKAQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            complement(2127..2687)
                     /locus_tag="O2692_001322"
     CDS             complement(2127..2687)
                     /locus_tag="O2692_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCZ4298957.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(2855..4762)
                     /gene="sasF"
                     /locus_tag="O2692_001323"
     CDS             complement(2855..4762)
                     /gene="sasF"
                     /locus_tag="O2692_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HCZ4298958.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTE
                     ITKEILSKQDLLDKVDKAIRQIEQLKQLSASSKEHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDASKDTSEDPAVSTTDNNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEAVKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSDLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            complement(5004..6362)
                     /gene="gtfB"
                     /locus_tag="O2692_001324"
     CDS             complement(5004..6362)
                     /gene="gtfB"
                     /locus_tag="O2692_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HCZ4298959.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFALFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            complement(6355..7863)
                     /gene="gtfA"
                     /locus_tag="O2692_001325"
     CDS             complement(6355..7863)
                     /gene="gtfA"
                     /locus_tag="O2692_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HCZ4298960.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISLAPTTYTLDDIERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNQDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            complement(7881..10271)
                     /gene="secA2"
                     /locus_tag="O2692_001326"
     CDS             complement(7881..10271)
                     /gene="secA2"
                     /locus_tag="O2692_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HCZ4298961.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENQDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            complement(10261..11220)
                     /gene="asp3"
                     /locus_tag="O2692_001327"
     CDS             complement(10261..11220)
                     /gene="asp3"
                     /locus_tag="O2692_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HCZ4298962.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQYDLEYKSMRVTKIG
                     DDQYGNDMINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDNIEGNIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLGCKVYRTSHEDDDLKGWLTEHVPGNHELKDT
                     NVEVYFKEEQDKHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            complement(11198..12766)
                     /gene="asp2"
                     /locus_tag="O2692_001328"
     CDS             complement(11198..12766)
                     /gene="asp2"
                     /locus_tag="O2692_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HCZ4298963.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLT
                     DDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            complement(12756..14309)
                     /gene="asp1"
                     /locus_tag="O2692_001329"
     CDS             complement(12756..14309)
                     /gene="asp1"
                     /locus_tag="O2692_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HCZ4298964.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            complement(14320..15531)
                     /gene="secY2"
                     /locus_tag="O2692_001330"
     CDS             complement(14320..15531)
                     /gene="secY2"
                     /locus_tag="O2692_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HCZ4298965.1"
                     /translation="MLKLLQQYEYKIIYKRMLYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKSISDDMPMLTF
                     DSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGLALVTVIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLNQYW"
     gene            complement(15661..22476)
                     /gene="sasA"
                     /locus_tag="O2692_001331"
     CDS             complement(15661..22476)
                     /gene="sasA"
                     /locus_tag="O2692_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HCZ4298966.1"
                     /translation="MSKRQKAFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTISIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSDSASKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDS
                     TSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSQSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSAFTSTSGSVSTSTSLSTS
                     NSERTSTSMSDSTSLSTSESDSISESTSTSDSISEAISASESTFISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSTSIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSDSTSLSTSLSDSTSDSKSDSLSTSMSTS
                     DSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTS
                     TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSSYTSQSTSQS
                     ESTSTSTSLSDSTSISKSTSQSGSVSTSASLSGSESESDSQSISTSASESTSESASTS
                     LSDSTSTSNSGSASTSTSLSNSASASESDLSSTSLSDSTSASMQSSESDSQSTSASLS
                     DSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDS
                     TSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSCSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTS
                     TSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSMSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     TSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTS
                     LSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGS
                     TSTSISESLSTSGSGSTSVSDSTSMSESNSSSVSMSQDKSDSTSISDSESVSTSTSTS
                     LSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHTHSTS
                     TSRLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGGVMTL
                     LVGLGLMKRKKKKDENDQDDSQA"
BASE COUNT         6795 a         4277 c         3555 g         7898 t
ORIGIN      
        1 atgcctaaaa ataaaatttt aatttatttg ctatcaacta cgctcgtatt acctacttta
       61 gtttcaccta ccgcttatgc tgatacacct caaaaagata ctacagctaa gacaacatct
      121 catgattcaa aaaaatctaa tgacgatgaa acttctaagg atactacaag taaagatatt
      181 gataaagcag acaaaaataa tacaagtaac caagacaata acgacaaaaa atttaaaact
      241 atagacgaca gcacttcaga ctctaacaat atcattgatt ttatttataa gaatttacca
      301 caaaccaata taaaccaatt gctaaccaaa aataaatacg atgataatta ctcattaaca
      361 actttaatcc aaaacttatt caatttaaat tcggatattt ctgattacga acaacctcgt
      421 aatggcgaaa agtcaacaaa tgattcgaat aaaaacagtg acaatagcat caaaaatgac
      481 actgatacgc aatcatctaa acaagataaa gcagacaatc aaaaagcacc taaatcaaac
      541 aatacaaaac caagtacatc taataagcaa ccaaattcgc caaagccaac acaacctaat
      601 caatcaaata gtcaaccagc aagtgacgat aaagcaaatc aaaaatcttc atcgaaagat
      661 aatcaatcaa tgtcagattc ggctttagac tctattttgg atcaatacag tgaagatgca
      721 aagaaagcac aaaaagatta tgcatctcaa tctaaaaaag acaaaaatga aaaatctaat
      781 acaaagaatc cacagttacc aacacaagat gaattgaaac ataaatctaa acctgctcaa
      841 tcattcaata acgatgttaa tcaaaaggat acacgtgcaa catcattatt cgaaacagat
      901 cctagtatat ctaacaatga tgatagcgga caatttaacg ttgttgactc aaaagataca
      961 cgtcaatttg tcaaatcaat tgctaaagat gcacatcgca ttggtcaaga taacgatatt
     1021 tatgcgtctg tcatgattgc ccaagcaatc ttagaatctg actcaggtcg tagtgcttta
     1081 gctaagtcac caaaccataa tttattcggt atcaaaggtg cttttgaagg gaattctgtt
     1141 ccttttaaca cattagaagc tgatggtaat caattgtata gtattaatgc tggattccga
     1201 aaatatccaa gcacgaaaga atcactaaaa gattactctg accttattaa aaatggtatt
     1261 gatggcaatc gaacaattta taaaccaaca tggaaatcgg aagccgattc ttataaagat
     1321 gcaacatcac acttatctaa aacatatgct acagatccaa actatgctaa gaaattaaac
     1381 agtattatta aacactatca attaactcag tttgacgatg aacgcatgcc agatttagat
     1441 aaatatgaac gttctatcaa ggattatgat gattcatcag atgaattcaa acctttccgt
     1501 gaggtatctg atagtatgcc atatccacat ggccaatgta cttggtacgt atataaccgt
     1561 atgaaacaat ttggtacatc tatctcaggt gatttaggtg atgcacataa ttggaataat
     1621 cgagctcaat accgtgatta tcaagtaagt catacaccaa aacgtcatgc tgctgttgta
     1681 tttgaggctg gacaatttgg tgcagatcaa cattacggtc atgtagcatt tgttgaaaaa
     1741 gttaacagtg atggttctat cgttatttca gaatccaatg ttaaaggatt aggtatcatt
     1801 tctcatagaa ctatcaacgc agctgccgct gaagaattat catatattac aggtaaataa
     1861 gtattatatt aaacccgtaa aatttataag tataaacaag gagttcggac ttaaacatat
     1921 ttctgttcat aagtctgatt tcttattcaa ttaaacccga ggcattcagt tcgaacgcct
     1981 cgggtcgttt tatataaata tattatttta tgttcaaatg ttcctcatca tatccgtttc
     2041 aattgccatc tcacacattt tataaatatg agcaaatgta cttattctca aacattactg
     2101 ccgagcttta attgacgtta tattaactat aaactacttt tccatgactc tacggattca
     2161 atgtcacatg agcgtgataa aatttgttca ataataaagt catgtttatc atctgatcta
     2221 tcaccaacag catcttctaa aacagtaata taatagtctt tatctacact ttctaatgcc
     2281 gtgctcaata cagctccact tgtagagaca cccgttaata ctaaatgatt aatatcattt
     2341 gcacgtaaat aaacttccaa gtaactacct gtaaatgcgc taaagcgtcg cttagaaata
     2401 atcggctcat cttctagtgg tgctaaatct tcaagtattc gtgtagatgc atctgcttca
     2461 gtaatcgcat atccttgagc tttaattgtt gaaaacactt tattactcga ggagacatca
     2521 ttaaaatgct tatctaacac taaacgtatg aaaatgactg gtattcgatg ttgtcttgct
     2581 gcttcaattg ctctctgatt cgctttaata atatttttta ttctaggtac actactcgct
     2641 ataccttctt gcatatccaa aactaatagc gccgtttttc gagacatctt cattctcctt
     2701 tacttgtgta gttctaagtc gttaaattca ttataacgtt aaaatgatgg acaatctatt
     2761 cattgcattt tgcatatact tcacaataaa ttaaggggaa ataagacgtc ttatatactt
     2821 aaaaaaatat atagatgctc ttcccccaat ataattatgc tttatttttc aacttattgc
     2881 gtcgtgataa ccaaatcatt agtacaccca ttgcaccaac aattacagat atcggcaacc
     2941 aatgttcttt tatcgtttcc cccgctttag gcaaagatac attaccatca gcatttaata
     3001 atccacttaa caatccatta cctttaccaa gtgttaacgt cttttctggc tttggtgtgg
     3061 gtatatctgg aatactgtct aataaatctg atccttgatt cattaaattt gctaacttat
     3121 ttaaatctgt cgttttccca tttttattca atcgatctag taaacttgga cgatttacta
     3181 ttggtgataa aatataatct atatcttttt tcgtttgatt gagtctcttt tgtaaattca
     3241 ataaatcatc cgctttacca ttcaatgccg atttaactaa attaaaaatt ttattttgat
     3301 ctgtttctat tttagtaatt aaatctgcca gtaattttgc cttttgtctt tctatacgtg
     3361 ttgctaaaat cgtttcaatt gcttgttttt tatctttggc attattcaaa attgctttta
     3421 atatatcatc tgaagacgtg tcgccagttg atgcaaaatg tttcttcaat tggtcaacga
     3481 tttggcgatt tgataatcct ttattcgtcc aatctttagc caatttatct gcttcagctt
     3541 ttcctaattt cgtttgtaaa atttgagaaa tcaatagcga cttatcttgt gattgatcaa
     3601 tcaatgacgt taataaatca tcactcgttg tcagagataa ttgatcaata tgacgagtaa
     3661 tttgatctgc aatttgttga tctgttttac catcaacacg tatatctttt agaattttaa
     3721 ctgcctcgtc tttattaaat atactttcta aaatgctttg tgtagcatac tttttatcat
     3781 cagtacgtgc aagttcttcc aaaataatat ttcgttgact ttttatacgc tctttcgtct
     3841 tatttacttc gctcattaag tctgattttt gatttttagg aagttgcgta tttgcaatac
     3901 gttgatctaa agattgtaac gtattcagtt tatgatatgt gtaatgttgc gttgaggcat
     3961 tacttttagc taatttttca atcatagcat gattaatttt atcgcttcct tgtaatttat
     4021 cagtgagtcg attactatgg ctttgattct cttcatttga aaggaattta tttaacacaa
     4081 catgtccaga accatcatta tttggcgttt tagctacttc atgattatta tctgttgtag
     4141 acactgctgg atcttccgat gtatctttcg atgcatcttt cgatttgtgt atttgctgat
     4201 tcaaatggtc taggtcttct aacgccttat ttaccattgc ttcatcattt ttatcatctt
     4261 tttctccatg ttttgttgta gccgtttgtg acatatcatt tttcattgca ttaagatcgt
     4321 cctcgccact ttgttgaccc ctatcaacat ttgaagaaac ctcatttaaa tctttaagca
     4381 attgatctaa tttactgtct atatcacttt gaccgttcat ttcagtgtga gaacttttat
     4441 tgtctttgct atccaactca ttagctcgtt ttatgatttc atctatttgc gatgctgttt
     4501 tcgcttcatt tagttgtgct ttataatgtt ctttagatga agccgataac tgttttaatt
     4561 gctcaatttg acgaattgcc ttgtcaactt tgtctaataa atcttgctta gataatatct
     4621 cttttgtaat ttcagtatcc ttttcagatg cagcttgggc atcgtacggc aagatattcg
     4681 ttaaaatgat acttgtcgcc atcattgtcg aacacgataa ctttacatat aattgaaacg
     4741 gtttccctcg atatttagcc atcaacatac tccttcctca cttacttcct tcaaagaatt
     4801 acatactatt atatacctgt ttacaagaaa tttacactta tctatctagt tattgttgtt
     4861 agtaattatc tacttattac ttagcttata tttaagtaaa caaaacaagc atgacgtaat
     4921 atcatattgt ccatgtcgct aacatcatat tacgtcaaat cttttatatt aaatgatgtt
     4981 ttattttaga ctgctttttc cttttagctt tcgagcgcct gtttaaaaac ttgctcgaat
     5041 tgttcacgcg agatttcgtg tgcatgtgct ttttgtgcta ataaagcatc tcgaaactgt
     5101 tgttgatctt tcaaactttc taacatttgt attaattggt ctttactttc cattgttatc
     5161 tcatcattat gctcaaataa gtgctctgat aatgttactt tagcatggtg tgcggtttga
     5221 cgataaccta aaatcaacaa ctcatagtca aacgcttgtt ccaccgcatt taaaatttca
     5281 ttaccctcat tgatatcaag ataaatatca cataactggt atagttcatt taccctgtca
     5341 atattaatag atgggtataa atgcacatta gcatattgat caagttgcat tagcttatca
     5401 gacatctctg taatagcagc gatgtgaaac ttaaaatctg gtaaagtttc aaccaatacc
     5461 ttgatgttac gaagttgatc cgagttagtt aatattacaa tttctttagt atatctatta
     5521 cgactacgat agttatatag atatccgcct tgtaaaatac gagattgaac ctttgcgtct
     5581 gctatattga gcatcgtttc atattcgttt ttatctggaa taataatatt acaatgtcgt
     5641 ttcatatcac ctttacacat caattgcata tttcccggga cattaccatt acagtgttct
     5701 tgccatacca aaacatcact accttttgat ggcaaattat ataacactga aaatggtagg
     5761 gctagtgagt taataacgaa atgatgttcc gtaatttcaa gttgcttgat aaaaaataat
     5821 gcgaatgcga gctttgaagg gaaaaagtaa gacttccctt gccaatccaa tatgacatca
     5881 gatgttacaa aattttcata aatcacttct ttaccttctg ctgtcatata tttcttcaag
     5941 atcgctttac gatttaaatc gtaaacagtt tgtgcaaatt taataccatt cttagaataa
     6001 taatcgacaa atcggacacg ttgttggtca tcaaaccatt cgacacgact aacaattcta
     6061 gggcgctctc cactttgata aaatatttta cctcgtagac gtcccatatc attaattgta
     6121 gccgaattgt tgttaccttt aatttcccaa aaagctggta cagtaacctg attaaaaaat
     6181 cgtggtttca tattttctgt attatgatta tctgcaaaaa attgatacgg tgatataaca
     6241 tcgtccggta aaaagccatt gtcattgagt acaattgtta aatcttcttc caacttactg
     6301 gctttaaaag actcatataa ctttcgtgaa tgatcgttaa agtaatcaaa taatttaatc
     6361 atgtagcacc tcttgaacta atgtttccca ttttaaaata atatcttgag tcataaattg
     6421 ctgtgccact tcataagaga tgtcatgtgg tgtctgggga ccattgttaa aatacattac
     6481 aatggcatga gctagttttg cgataacatc atccacacta tcttcgtcgg tatcaaaagg
     6541 taccaagtag ccattttccc catctcgaat aaaggttggg ttaccataat tcacatttaa
     6601 tccaatcata cctagtcctg agcctaccgc ttccattagt gttaacccaa aaccttcgct
     6661 agttgatgca gaaagaaata actcataatc attataaatt tcatcaagtt taacatgccc
     6721 tagtaaacga atataatctt gtgcgcggtg tgtatcaata attttacgca gtcgcgtctt
     6781 ctcgctacct tctccataaa tatcaaatgt taattctggc acttgtcgtt tagccacgat
     6841 aaccgctttg acaagccaat caatatgttt ctcattcgct aaacgagatg cactaatcat
     6901 cgcatatggc tttcttgata atgttggata tgataatgca tcaatgcttc ccacaggaat
     6961 agtataaaca cgtgggcgat aaccttgata ttgctcaaat tgtcgacaaa ccatatgatt
     7021 ttgaatatct gttgctgtaa taaagaaatc aatgtattta gcttttgaaa attgatattc
     7081 ataataattg ttccatagta tatgctgctc actcatcata ttattactat aatgatcagc
     7141 atgaatcaca acaccaactt tactatcacc tttatgctgc aaaacagcct gaccaatatc
     7201 agaagcgcgg tctaatatga caatatcgtc tcgggttaaa ttcaatcgtt gtaaaaagta
     7261 tgcaataaat tccgttttgt tatacaacac cgcatcttca aacacatata tagagctgtc
     7321 tccatcaata tattcgttat aagcgatgga accatcttga ttataaaatt gtcgcatata
     7381 taatttcgct ttattatcag ctggtgcata atactcagaa aatatgcgcg tataactata
     7441 aaaatcttta cgtactaaca tactattaat tacaaattct gcacgatcca caatatcttt
     7501 ttgttcattt tgcagataac atgttacaaa tgatgatttc ccattaaaat ataggcggac
     7561 tatcttacca tttctttctc taaaactaat gtcatgacca agctcacgtt caatgtcatc
     7621 taacgtgtac gttgttggtg ctaaagaaat atcactaaaa tactgataca accaaataac
     7681 ttcttgatct ttaaacccaa tgttttgcgt taatgtctgt atgttctctg actgtataaa
     7741 atctaaaaac acaaatttag tgtcttgatt tgtacgtctc aataatttag cacggtaagc
     7801 ttgtgcatat tcaacaccgc tactcgccca gcctatacca aagtttatat tatatattgt
     7861 catgcgctac cccttttcat ttatggaaaa tgtataactg gcataccctc tttatcaaat
     7921 gtaatcatgc tttgacaaat atttttcacc attctttttt tgatatttcg tgtcataact
     7981 tcaaatgaat ctaaggcaac tctatggtat tcaaaaatag cgttacgttg cccattttgt
     8041 cgttgattaa cgcttgcttt taattgttgt aaatagtcga cttgttctaa ccaacatgaa
     8101 tcaattgctt tcaaaaagac tttttgaacg aaaatattat aataatatgc actttgcatg
     8161 tttttacgat tcaaagctaa ttgcttttca aattgctcta ataaaaatgt cactactgct
     8221 tgcttatctt taaaattaac acaagccaca tctttattaa attggaaact taaattttga
     8281 taaatatact cgacaacacg cgattttgtt agcacctttt cctcatttac aaacatttca
     8341 aatacatctt tagctaacgc tttaaaatct tgattctcag catcatctat ttctaaaact
     8401 cgattgcgtt cctcgtatac aagatctcgc tgtatactaa tgcttttttc aaattcatta
     8461 gccatttcac gagctttaac cccttgttct tccgagatac gctgcgcttt aactacaatt
     8521 tgcttaactt tgcgattaaa caaattactt tgcgataatc gttgtgcatc taatgaatat
     8581 aattgattat tttccgctaa attactatcg ctccatcgct taactaaata atcatctagt
     8641 gaaatatata tacaagatga tcccggatcc ccttgtctac cagaacgacc acgtaattgc
     8701 ctgtctacac ggctattttc catatgttca tgaataataa cagctaatcc acctaatgct
     8761 tcgacacctt caccaagttt aatatctgtg cctcgacctg ccatactagt cgcaacagtc
     8821 atggaaccaa tttgccctgc ttcagctatc atctgcgctt cttttgcaac attttgcgca
     8881 atgagtaaat tattaggaat atccatttgg aataatactt tcgaaaagta ttcagccgct
     8941 tcagcagttc tcgttatgag taaaaccggt cgccccgttt catgaagttc aactatatca
     9001 tgaatcatcg cgatgttttt ctcatcaact gaacgaaaca ctttatctgg ttcatcgata
     9061 cgttgaatcg ctttatcagt tggtacttgt acgactattt ttgaatacaa atcaaagaac
     9121 tctgattcgc ctaattttcc tgtagctgtc atacctgaaa atgattcaaa aagtttaaat
     9181 aaattctgga aggtaattgt tgccataaca cttttatctg ttgaaacctc cataccttct
     9241 ttcgcttcaa tagcttggtg aagtccagct tgcaacttag ttcccggtaa catacgacct
     9301 gtaatacggt caattaaaac aatatcacca ttatatacaa agtaatcgac attagattca
     9361 aacaaatatt gtgcgcgcag tgctaaatta atattacgca ctaggaccat cgcttgttcg
     9421 ctatataaat cttcaacatt aaagtatgat tgtgccgctt caataccttg atttaacagc
     9481 catatttctt ttttggtctt cttcatttta aaatgcacgt cttcaatcaa tgtatctaca
     9541 aactctttca caatatgaaa tagatttgat tgtaatcttg gtgcacccga aataactaat
     9601 ggtgtttgag cagcatctaa aatgattgaa tccacttcat caataatacc gtaatttaat
     9661 tgtggtaaaa atttcccttc cgcactatca gccaaattat caattaaata atcaaaaccg
     9721 agacgtccat tagttgtata tataatatca tgttcatata tattacgttt ttcccctttt
     9781 tgatactcat aatccacaat atcaacaaaa cctaatgaag cagttaaacc taaccattca
     9841 tataatggtt gcatctcttc aaaatctcgc ttcgctaaat aatcattcgt tgtaattaaa
     9901 tatgttcctt ttcccgaaag agcatttaaa tataaaggca tcgttgccgt taatgtttta
     9961 ccttcgcctg tttgcatctc cgcaatgtta ccttcatgca atacaatcgc tccgattaac
    10021 tgaacttctt taggatacat acctaatact ctccagctcg cttcacgtgc cactgcataa
    10081 gcttcaggta acaatgtatc tagtgtatca actcctgatg ctaaacgttc tttaaattct
    10141 attgtctttt gttttaacgc atcatcagaa tatgatttaa cttcatcgct ccatgtattg
    10201 atgcgtttca ctatttttct aatcgacttt agtcttaatt cgtttatcgt aacatctagt
    10261 ttatgtttca tttacttccc caccattcag tttcgataca tctaagtaat ctaaaaatcg
    10321 tactggattc attaaacgtg acatataatt tagatgtttg tcttgctctt ctttaaaata
    10381 aacctcgaca tttgtatctt ttagttcatg atttcctggg acatgttctg taagccatcc
    10441 ttttaaatca tcatcttcat ggcttgtacg atacactttg caacccaaat gctgagcgac
    10501 ataagttgca aaaacatttg actttgaccc ataactaatc aaattaatag cctttagggt
    10561 atcttgactt tgcaaatcat tctttagttg cttaatattt ccctcgatat tgtcgtccat
    10621 ccaacgttca acgagccaaa catgaccaaa cagtttcaaa aaatcattcg aaatagttgg
    10681 ataggtgtca gatggttctg caataatgac attgatcata tcatttccat attggtcatc
    10741 gcctatcttc gtcacccgca tgcttttata ctctaaatca tattgatgcg tcatctctgt
    10801 gattgttaaa catctaaata taagactcgt cgatgctgca ttcatcattt ttattttata
    10861 agcataggct tcatcaggat attgaatcgt aatactattt gactttacaa tctcagtact
    10921 tagttttgtg ccatttttat tataaaaaat gatgataaaa tacactgaac cagcaggcgt
    10981 tgcatcaaaa tcaaaatgca atttataatg ctgtcctcta cgcaaaattg gtaaacttgg
    11041 cgcactttta tattttgaaa attgctttaa catcaaccac tcatgaatcg gtaatccaga
    11101 gggcatcaaa ggatttataa aagtcacttc accatttgaa aatgatactt tagagccata
    11161 cataaatgta gtttgtgaaa tataattcca agtaacttta aatgttttgt ttttcagcat
    11221 gttgaactct cccaaacttg tcttccaaaa taatgttgta aaaattaaca aaccaacttg
    11281 caatggtagg tgaatcatca ttatgtcgcc caggaatact gcgattcatc actcttgctt
    11341 ggtgtgctgt caatacaggt aatagctctt gaaatgcatg tggatcataa tcatcatgtt
    11401 gcatatatgc tatggcaaaa acagtttgtg acaatgattt cttttgaaat gtttgccaaa
    11461 atttttgatt taatgcctgt atcgacgctt gagatgtatc accttcatta gacaccagga
    11521 cgtctaatgc tgtaccgaac tcttctggtc taagtaatcg catatgttca gcaatcgttc
    11581 caatattaac aagtggttta ccaacaataa ttgcctgagg atttaactga gcaccataat
    11641 acaaggcacc aaatgaaccc atagataaac cagataatat taattcatgt gatttaaaat
    11701 tcagcttttc taatgtctcg tcaataacat tgataatacc ttgttcatat tcagatgaac
    11761 cgatataaaa actaccacct tcaacacgag gatcgccgat aagtaaaaac ggtgcattca
    11821 tacgtttcat catataatat ccttcgaaac cttccgctgt tcgataacca ctaaaatata
    11881 cgtttagtgg cggtttcata tcaccagggt ggaaataata aataaattcc tgtcgttgac
    11941 tatctacgaa acgactacca ccaagtaaaa attgacccat gtctaatcta gaccatcgtt
    12001 tgtgtatagg tcctaaatgt accgtcccgt tcccacgcgc cttaacagtt acacttatat
    12061 aagcatcaaa tggtttcgca ggtatctcta aaggactgtc taacatatca tcagtcaata
    12121 cgatttgttc aattaatgca ccatcagcgc cagtctgaat caatctaaat gtatattgca
    12181 actcgaccgc accatcaata tcaaattctg gccatatttg aatgacttta tctttatcgt
    12241 aaacgagatt attttgccaa gatgcgatag gtttaaattc tttcccaaat tctccactca
    12301 atgtgagctc tgaattacct tggtaaacga catctccttt aaaattcgga tgcacaagtg
    12361 ctaacttagg agaaacctta tctccatact gtcctgagaa gctaactgcc tctaatttat
    12421 tattacgttc ttcaatattc cggtaatgta atggttgaac aacgtatttt tggacatttt
    12481 cgtcttgttc atattcaact gaccaaaatg attcatcaac atacgtattg tatggttcgc
    12541 ttatcatttg taataaattc gttaatgtct ccgagtatgg tgcttgaata tagataaaat
    12601 caaagcgccc ttctgcttca acaatcgctt caatagcctc tacataacca ctatcaaatt
    12661 caaacaatcc aatatcgaag taatcccaac tcacaccttt tttgtgttga aaaataggtt
    12721 ctaaatcgtc tcctccaatt tgcaaaactc taaatttacg tggcatcatt ttcaccttct
    12781 attaactcat cgagctgatt aataatattc ttagaagcat atgcatctat taattttaaa
    12841 gaataggcgt acgcataatt ccaatttttc aaataaaata aataataatt taacgcatca
    12901 tctaattcat caactgtatt tataatacgg ccattgtcat aatcagagac gtaatctgtt
    12961 tgttgaccat taatttgtgg aatcccagcg ctaattgcac taatttgtaa atacaagtca
    13021 ggttcttttg acatatctat cacaagtcgc aacgtccgca atgcttctac aacatcatgt
    13081 tcagcatgta tcgtcttaac agcaatgatg tcatcttgat cttcaggtgt cattaatgct
    13141 gaaacattaa catccgcatt ctgtttagct tggtattcct catttaccga cgtaatacat
    13201 tcacgaagcc acatcggtat gtcattttga tggcgcgata ataaaattaa acggtaataa
    13261 tcttcctgtg cgatataatc cacaagtcgt tgcatcattt gttgcaaatc agcgtcactc
    13321 ataccatcta tccatacacc tataaatgtt tccatcaatt gactacttat attaggtgat
    13381 tgtctcgttt caaatggtgt gattcgaatc attgtattct cccgctgata cttctcttga
    13441 tgactaatta aatcacgttc tagttgcaca ctatcaacaa ttaaatgggc attctttacg
    13501 atagattgat attcctcatc tgacacagtt tcatttctat ttttaaaaaa tgaataactt
    13561 aatgatttcg ctggaatatg attggctatt tgtcgattgt gcctagcatc tgaagccaca
    13621 atcacatgat catcttcatg tatttgttgt gcaatcattg cttgaaattt ttcttcaatt
    13681 agttgagcca tattgttata ttctgtttgt tgatagtgat gttgatatct ttttgaaaca
    13741 gtgactctgc catttttcaa atcttcatga agtacacaat ctccattaat cgttaaatat
    13801 tcttggtaag aagcctctcc ctgatcatca aaataacgta tcgctgataa ataacctctg
    13861 tcatcaaaaa tataacgccg ttgtaactga tctctttcaa attcttcaaa ccaaattgaa
    13921 tacccttctt gactaaaata aatatttgta taggtctgtt cactcgtcac acattttaat
    13981 aaatacggtg tgtacacaaa ctcaacatca tccggccatt ttaagtgatg ataattaatc
    14041 gcttgtggcg catggtgact gaatccttga atttcatcaa acacagacga atactttgtc
    14101 tcatataagt catatcgatg taaaaatgtt cttaaatttg gtgcatgatt gagaacaatc
    14161 agttgataat ctaagtcatt ttcaaggtgc attcccatta aactaatcat atcgtcaaat
    14221 tccgtcttat tttgtagttg ataatacggc acagtcgtgt cttgccacca tcgttggtca
    14281 tcgtaccaag ctggaataaa gtatttcata attacctcct taccaatact ggtttaaaaa
    14341 tggcttatat ttatcaaaat ataaatatgt acgaattgtt tctgcaatat taatactgat
    14401 gtaaactaat acaatcagtt gtactgagaa ataaatttca gtagataaat gcggtacaaa
    14461 caatgtgaaa taaagcggta taccaataat gactgtaact aatgccaatc caaaccaaca
    14521 tacgcgtcgt gcttgataat ttaaataacg ttctgtatcc ttaccaggtt taactcctga
    14581 aaaataattg ccactcttta agaaatcttt ggatttttgt ttagtattga ttaaaaatct
    14641 cgataaaaaa taacccaata acatttgaat cactaaatat actgaaatac ctactggact
    14701 atcaaatgtc agcattggca tgtcatctga tatgctttta ttaaacatag ataaaataaa
    14761 atgaatgcca ctttttaaga aaacaaaagc tgaaatactc atcattaaag taatactgcc
    14821 tgcagggtta actttccaag ataaataaga tttcatattt gttgcggaaa cgttcattaa
    14881 atcgatatat ggtattctca cttctactaa ttcaataaat aataagataa acaatgtgat
    14941 tatcacaagg atgattaaca acgcaatcac aatatgactt gcatctatat attccatttt
    15001 ttgatgcatc attgatttaa taatactaac cattacaatc ggcattggtc ctgcgatgcc
    15061 gtagcgacta tttttgtcag ctaaccaaac taataacatc gttccagtaa ccaaaatcaa
    15121 tattgttaag taaatattgt cttgatgaac acgttctttc gaaacatatt catgaatcac
    15181 aaaataactt tgaataacac ttaaaattaa tgttaagatg cgctctttat aatgcttctc
    15241 aagacttgtt tgtttcatgt atttatccat attccgataa gaaatcagca tcaagataat
    15301 cattgatgtt aaccacggac ctaatcctaa agtgaaaatg tttaaagtat taacgtctcc
    15361 acccatatta gaaatagcta ttttaaaaaa tgactcatgt tttacttgca tatcgttata
    15421 ggaaacgatg gaaatgtttg tgcctaatat ataaataaac aagataaaac atgtgtatag
    15481 catacgttta tatataattt tatattcgta ttgttgtaaa agttttaaca tgttgcacct
    15541 cttttatatc aaaaacatta aaaagactaa gggttcatca ctaattatta aaatcctata
    15601 tcgatttttc tagtgattgg tgcctcagtc tttttaattt tagccagcta taaattcaat
    15661 ttatgcttga gaatcatctt gatcattttc atctttcttt ttctttctct tcattaaacc
    15721 taaaccaact aataatgtca taacgccacc tagtaatcca ttttgtttta ttgagtcacc
    15781 tgtatctggc aatctttttt cactttgtgc tggtgtgcca ttatgtttag tcacttcaga
    15841 tgttgcactt aatgtagact gagattcact cgtgctcgtt gttgcttcac ttgataagcg
    15901 agatgtgctc gtgctgtgag tatgatgcat actcattgag tctgacggat gcattgagtt
    15961 agattcggat gtacttgttg agccggacat acttgttgat gttgagtcag aaatgctttg
    16021 tgaaccagac atagatgtac tcagtgattc ggatgtgctt gtcgaatcgg atgtgctcaa
    16081 tgacgttgat gtgcttgttg acactgattc tgagtcacta attgatgttg agtcggattt
    16141 gtcttgtgac attgaaacac tcgatgaatt agattcactc attgatgttg agtcagatac
    16201 gctcgttgaa cctgaaccag acgtacttaa tgattcagat atgcttgttg aagttgaacc
    16261 acttgttgag tccgatgtac ttgtcgatgt cgagtctgaa tctgatgtac tcaatgattc
    16321 tgagtcactg atagaagttg aatcacttgt agattctgat tctactgtac tttgtgaacc
    16381 actgatactt attgaagtag aatcactgat actgtctgat gttgataatg atgtcgacac
    16441 cgatgtgctt tgtgatgacg atgtactagc actcattgac attgatgttg atatcgatgt
    16501 acttaaggaa ccagatgcac ttgtacttgt tgactggctt tgtgacattg aatcacttaa
    16561 tgatgtagat gtgcttgttg agctcgagtc acttacactt gttgaacctg atattgagtc
    16621 acttaaactt gtcgatgttg aaactgatac gcttccgctc attgagtcag atgttgaaag
    16681 tgatgtactc gttgaatttg atccactgat gctagacgaa tcacttgtag acattgagtc
    16741 gctttctgat gcactgatgc tcatagagtc aaattgacta ttacttgttg agcttgactg
    16801 cgaatcgctc acacttgttg acgttgattc tgatccactc atactttgcg agctactcaa
    16861 tgattttgaa tcacttaatg aatccgaagt gctaagactt gtggaaccac ttaaagatat
    16921 tgatccactt aatgagtcgg agtcacttgt actagtagaa tcactcattg atattgaatc
    16981 acttagcgag gtagactcgc ttacgctttc tgaaccactt aatgatgttg aggtactcaa
    17041 tgaaccagat gtacttgttg aagtcgaacc acttgttgat tttgaatcac ttaatgaatc
    17101 agattcactc acgctttctg aacttcttag tgacgtcgat acacttaatg atgacgaatc
    17161 gcttgtgctt actgaatcgc tcatcgattg tgagcaactc aatgaacttg actcgcttac
    17221 actttctgat tttcttaatg acgttgagac gctcaatgag ccagaatcac tgacacttgt
    17281 tgagccactc atcgatttag agtcactttc agaattagat tcacttacac tttctgaatc
    17341 atttacagat tctgacatac tttgtgaatc agatatgctt gcgctcatta cttcactagc
    17401 cgatgttgat gtacttgtcg aatcacttaa cgatatagac acactcatcg aaccagatgt
    17461 actcgcactt gttgagtctg atgttgaatc actaacacta tcagataatg acgttgaatc
    17521 actcatactt gttgatgtac ttgtcgaaag cgacatactt tgtgaatcac tagtacttgt
    17581 acgcatcgaa gtactagttg aagctgatgt actacgagag tcacttgttg atgttgatgt
    17641 acttgctgat cctgatgcac ttgtacttct tgatgtgctt tgtgaatcgc ttaatgatgt
    17701 tgatgttgaa tcggattcac ttgtactttc tgatgttgag ccagactctg atgtacttac
    17761 cgatgtagat aaacttgcaa tggtcgacat gcggtttgaa gttgatgtac ttagcgaatc
    17821 acttaatgat gctgatgtgc tttgtgaatc ggattcactg ctttgcattg acgcagatgt
    17881 tgaatcactt aaagatgttg acgacaaatc ggattcactt gcgcttgctg agttactgag
    17941 cgatgttgac gtacttgctg atcctgagtt acttgtactt gttgagtcac tgagtgatgt
    18001 tgacgcactt tctgatgttg actcacttgc acttgttgag atactttgtg aatcagattc
    18061 actctctgaa ccacttaatg acgctgatgt gcttaccgaa cctgattgac tcgtactttt
    18121 agatatactt gttgaatcgc ttagtgacgt tgatgtcgat gtggattcac tttggcttgt
    18181 tgactgactt gtatatgatg atgtgctttg tgaatcggat tcgctcgtgc ttgtacttgt
    18241 tgagtttgag gcactttggc ttgctgagtt tgagtctact ccgctttgat tcattgaggc
    18301 acttagtgac aatgatgtac tcgttgagtc agacaaactt gtactcgttg acgtacttgt
    18361 acttcctgat gttgattgag acatacttat gctcattgat gttgaatcgg atttactttc
    18421 acttgatgat gttgagtcgg attcactttc acttgtagaa ccacttaatg atgtggatgt
    18481 actaatggaa tcagatttac ttgtactgat tgaatcactt gtcgacattg atgtacttaa
    18541 tgaatcagac ttactatcac ttgtggaatc acttaatgat gttgacaaac ttgtagagtc
    18601 agacaaactt gtactcgttg acatactcag tgatgttgaa acactctcgc tcttaaacgt
    18661 tgacgttgat tcactgatac ttgtcgatgt tgaaatggac gtactaccac ttgttgaatt
    18721 acttaatgat gttgatgtgc taccagattc tgatgtactg tctgataatg acgtactctc
    18781 acttgtcgaa ctactcactg actctgatgt tgattcagac gtactttcac ttaatgattc
    18841 acttaaaaag gcagatgcac tttgtgattc tgaatcgcta gtactatttg attcacttaa
    18901 tgatataaac gtgctctctg aagcagatat tgcttcactt atagagtcgc tcgttgacgt
    18961 tgattcactt attgaatcag actctgatgt acttaagctt gtggaatcac tcatagatgt
    19021 tgatgtacgt tctgaattac ttgtacttaa cgatgtcgaa gtacttaccg aaccagatgt
    19081 gctcgtaaaa gcactttgtg atattgattc acttgatgca gttgatgctg atttgctatc
    19141 actcgttgaa tcactaaacg acgttgatat gctcattgaa tcggattgac ttgcactcat
    19201 tgaaccagac gtactttgtg attccgaagt acgtactgaa gcacttgtcg acgttgatgt
    19261 acttgttgaa cctgattgac tagtactttg tgataatgac ttactatcag aatcagatgt
    19321 actttgtgaa tcacttaatg attctgatgt actacctgac tgagacgtgc tcattgaact
    19381 acttacggac attgatttac tgtctgatgc agataatgac ccacttgtac tgatagagtc
    19441 acttactatc tctgaagtac tcatcgagtc tgatgtactt gttgagacac tttgtgatgc
    19501 tgctatgctt agtgatccag aaacagaacc acttgtgctc gtcgaatcgc tcaatgattc
    19561 tgatgtactc atcgattttg aatcacttgt acttaatgat attgatgtac tttgtgaatc
    19621 tgatttgctt gttgacgcac tttgagagtt ggctatgcta tttgaaatac tgatagagtc
    19681 cgaggtgcta gctgactcgc tcaatgatgt tgatgtacta attgcattcg atgtactgtc
    19741 acttaatgat gctgatgtac tagacgaccc tgatatactc gttgataagc tttgtgactt
    19801 agacaagctt cctgatgtac tcatacttaa tgagtcactg agtgatgttg atgtacgcaa
    19861 tgaatcagat gtacttgttg atagactttc ggatttttca gtactgctag agtttgaaat
    19921 agaatcgctt aatgatgttg atttactagc tgaatccgac atgcttgatg atacactttg
    19981 tgaattcact aaacttgtgc ttgttgagct tgatacacta ttactttcag atgtgcttaa
    20041 tgacttagat gcactcacag aatcagatag gcttacactt gtcgatttcg aggtactagc
    20101 tgatgtagat accacaatcg atcctgatgt actcgttgat gcactttgtg agtcagcttt
    20161 acttgttgac acactttgag attgttgtgt acttcctgat gttgatacgg aatcactcat
    20221 gctatttctt gttacttcat atttaaaagt tgtcgtcgtt ttgttaccgc tcgcatctgt
    20281 agaaacgatt gatatagtac ttgtaccaat gtttgttggt gtaccactaa tagtattatt
    20341 tgtactatca aatgttagtc cggatggcaa tccagtcact gtattcgtca ccgcatttcc
    20401 actgttatct tgcgtagcaa ttttaatcgg ggatattggt gaatacactt ctgatgattg
    20461 atctcctatt ggtgtcactg ttggtgctgt cgtatccaca acatttattg taaaagttgt
    20521 cgtcgatttg ttatttgctt ggtcagtaga cacaactgtc actgttgatt gaccaatttt
    20581 tgttggtgtc ccaatgattg aattcgttgc actatcgtaa cttaatccgc ttggtaatcc
    20641 tgtaactgta tttgtcacag tcccagtacc attatccgtt gtagtcaata caataggatt
    20701 cattgtttta cccacttcta tggtttgatt gcctacagtt acagttggtg cttttacatc
    20761 agtaaaataa tatgtcactg attgtccagc attcgtcatt tttacagttt tatttgtatc
    20821 attataagtt gacgcatatg aactatcgac ggacgtgtag ttatatcctt tagcagtcaa
    20881 tgcagattgc tgattatcga ttgtcacgac ttgatcaaca tttcctgaat atgtttttgg
    20941 tggaataata tctttacctg ttgttacatc aacgtatctc acttgtgtaa cagcagactc
    21001 tgtatattcg aatgttccaa attgtacttg ttgtaaattt gtcgcgccac ctgttgaggc
    21061 tgtcattgat aatgaaaagt tggtcgtacc acttttcgca atccaatctg aaatattacg
    21121 tgtccatgtt tgacctgcat atttgacagt cataaccttt gtatcaccat tatagttaat
    21181 atcaaaatct tggaacgtgt tatttgtagg ttgaacattt aacttcgcag cattatcagc
    21241 tgttgaactt gatgtatacg ttgtcgcaac accataacta tctgttgtta caaatgcacc
    21301 aaacgcacct ccaccagcta cattagatgg gtcagcattc gcctttgcag ctgaatttgg
    21361 tttagatgta ttgtgatacg tatccaattt gaagccaaat gcgttactta agccaccaat
    21421 acctactgcg gcaccgttta accctgtttc acctaataca cctggtgaaa aggcaaaacc
    21481 gataccatct ccaccatttc catgcccttc atatttgtta cctaaattta cttttccaga
    21541 aaaatgaaaa ctcttattag agtcaatacg tgttcctaat gtaatagcac ctttttggct
    21601 gtatgcatcc tgtgttaacg tcacaatacc ggtactttga tcataggtag cattacctga
    21661 cgttgtcata tattgtttta agttatcttt attaactgta attgtattag cagttactgc
    21721 ggttgtcgtc gctgctgacg caaatgttga catagctaag cgactgaaag ttcgaagttt
    21781 tactggtgcg gtgctagttg acgttgtgct agttttgttt aagttgaccg aagatggcgt
    21841 tgtgctttgt gaagtgttat ttgatgcagt actttgattt gttgatgtat taattggttg
    21901 ttctgtactt gaagttgaag ctacagattt agtatcagaa cttgatgtag tattctttga
    21961 ggatgttgat tctgatgtag atgtcaattt ctcttgttga ttgcttgtac tattagttgt
    22021 cgaagtgacc ttttcagact tttcacttga gactgtgtca ctatttgatg tttgtaccga
    22081 actactattt ttcgttacac ttgtggaatc ggctgttgat gttgatgctt cgattgtcgt
    22141 tgagttttga ttacctactg tttcactttg tgtgtttaat tcagaagtta atggtgcatc
    22201 agaagccgca aaagcttgct ggtcatgcaa catatttacc gtgaatgcac caccaataac
    22261 cgccgtagtt ttcagtccgt atcccgtcat ttttttacta atgctttgat tatcttgact
    22321 cactaaacta tgactaataa atggtagccc cataattttg aacatttcta tttctttaat
    22381 tccggatttt acccaatttt ttccagattt ataaagtctt actcttgttt tttcgtttgc
    22441 taagctgtca tgaaatgctt tctgtctttt actcatgtaa taactccttg tattatcttt
    22501 acattcatta gattataata tatgc
//
LOCUS       DALAGF010000038        22030 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.038, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000038 DALAGF010000000
VERSION     DALAGF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22030
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.038"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..860)
                     /gene="folD"
                     /locus_tag="O2692_001332"
     CDS             complement(<1..860)
                     /gene="folD"
                     /locus_tag="O2692_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCZ4298967.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            1061..1543
                     /gene="purE"
                     /locus_tag="O2692_001333"
     CDS             1061..1543
                     /gene="purE"
                     /locus_tag="O2692_001333"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCZ4298968.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            1530..2654
                     /gene="purK"
                     /locus_tag="O2692_001334"
     CDS             1530..2654
                     /gene="purK"
                     /locus_tag="O2692_001334"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCZ4298969.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            2658..3362
                     /locus_tag="O2692_001335"
     CDS             2658..3362
                     /locus_tag="O2692_001335"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCZ4298970.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            3362..3625
                     /gene="purS"
                     /locus_tag="O2692_001336"
     CDS             3362..3625
                     /gene="purS"
                     /locus_tag="O2692_001336"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCZ4298971.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            3627..4298
                     /gene="purQ"
                     /locus_tag="O2692_001337"
     CDS             3627..4298
                     /gene="purQ"
                     /locus_tag="O2692_001337"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HCZ4298972.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            4291..6480
                     /gene="purL"
                     /locus_tag="O2692_001338"
     CDS             4291..6480
                     /gene="purL"
                     /locus_tag="O2692_001338"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCZ4298973.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNISLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            6459..7943
                     /locus_tag="O2692_001339"
     CDS             6459..7943
                     /locus_tag="O2692_001339"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCZ4298974.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            7936..8964
                     /locus_tag="O2692_001340"
     CDS             7936..8964
                     /locus_tag="O2692_001340"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCZ4298975.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDVSRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            8967..9533
                     /gene="purN"
                     /locus_tag="O2692_001341"
     CDS             8967..9533
                     /gene="purN"
                     /locus_tag="O2692_001341"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCZ4298976.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            9548..11026
                     /gene="purH"
                     /locus_tag="O2692_001342"
     CDS             9548..11026
                     /gene="purH"
                     /locus_tag="O2692_001342"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCZ4298977.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPSGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            11048..12295
                     /locus_tag="O2692_001343"
     CDS             11048..12295
                     /locus_tag="O2692_001343"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCZ4298978.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQAILDFAKRQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYKK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(12560..13366)
                     /locus_tag="O2692_001344"
     CDS             complement(12560..13366)
                     /locus_tag="O2692_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCZ4298979.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG"
     gene            complement(13359..14759)
                     /locus_tag="O2692_001345"
     CDS             complement(13359..14759)
                     /locus_tag="O2692_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4298980.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQLP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(14774..15349)
                     /locus_tag="O2692_001346"
     CDS             complement(14774..15349)
                     /locus_tag="O2692_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCZ4298981.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(15426..15526)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15933..16067)
                     /gene="graF"
                     /locus_tag="O2692_001347"
     CDS             complement(15933..16067)
                     /gene="graF"
                     /locus_tag="O2692_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HCZ4298982.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     gene            16219..17514
                     /locus_tag="O2692_001348"
     CDS             16219..17514
                     /locus_tag="O2692_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298983.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THAHNVWEFLNAFFSEIIKVMSRFIGNFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHF
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     SGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            17939..19111
                     /locus_tag="O2692_001349"
     CDS             17939..19111
                     /locus_tag="O2692_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCZ4298984.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQQINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSAIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            19165..19707
                     /locus_tag="O2692_001350"
     CDS             19165..19707
                     /locus_tag="O2692_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HCZ4298985.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISNAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            19861..20127
                     /locus_tag="O2692_001351"
     CDS             19861..20127
                     /locus_tag="O2692_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCZ4298986.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            20130..21848
                     /gene="ptsP"
                     /locus_tag="O2692_001352"
     CDS             20130..21848
                     /gene="ptsP"
                     /locus_tag="O2692_001352"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCZ4298987.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
BASE COUNT         7742 a         3170 c         4153 g         6965 t
ORIGIN      
        1 tacgaatcaa tacctcgacg cattttttct gcaagcaaag tattatttaa taccatagta
       61 attgttaatg gaccaacgcc accaggaact ggtgtaatag ctccagcaat ttctttaacc
      121 gcatcataat caacgtcacc ttttaatttg ccattttcat ctggcgtatt gccaacatcg
      181 ataattactg ctccttcttt gaccacatct tttgttacta aaccaggctt accaactgca
      241 ctgacaatga catcagcatc ttttaaatat gatgccatat cttttgaacg agaatgtaag
      301 attgttactg atgcattttt ttgaagtagt aacttagaaa ctggttgtcc gacaatatga
      361 cttcgtccaa ttacaactgc atttttacct tctaaatcaa tatcagcatg ttttaatatt
      421 tccatgatgc cgagcggtgt gcaaggtaca aaagtttgtt catcgatata taatttccct
      481 atatttattg gatgaaaacc gtccacatct ttttcaggat tgattgcttc taatattttc
      541 tgttcgctaa cttgttttgg taatggtact tgtaccaaaa taccacttac agaatcatca
      601 ttatttagtc tatttagttc gtttaatact tcttcttcag tagctgtttc ttccaaatgt
      661 acgatttctg aaatcatacc aattttttca gctgctttct tttttgatct aacataactt
      721 tgactagcgc catcattacc aactaatata acggataatt taggtgtaaa acccttttct
      781 tttagcgctt caacttgatc ttgtaacccc tgtctgtagt ctttggcaat ttgtttacca
      841 tctaaaattt tagcaaccat aaaaacttct cctcctattg tcaatatcct aactttgtta
      901 gtttataaaa ctaatttaca tagcaaaaga cgtaaattca aacaaaaaat caaaataaag
      961 ttcgattttt gattgaaaaa gcagaaattg cttgttatgc tatatctata atatacaact
     1021 aaagtaattc acaattcaaa cttttgaaag ggtgtacaaa gtgaaagtag cagtcattat
     1081 gggcagttct tccgattgga aaattatgca agagagttgt aacatgttgg attattttga
     1141 aattccgtac gaaaaacaag tagtatccgc acatcgtacg ccaaaaatga tggttcaatt
     1201 tgcttctgaa gcgagagaaa gaggtataaa cattatcatt gcaggcgctg gcggtgcggc
     1261 acatttacca ggtatggttg catcattaac gacgctacca gttattggag tgccgattga
     1321 aacaaaaagt ttaaagggta tagattcttt attatcaatt gttcaaatgc caggaggtat
     1381 tccggttgca acgactgcaa ttggtgcagc aggtgctaaa aacgcaggta tacttgcagc
     1441 aagaatgtta agtattcaaa atccttcttt agttgaaaaa ctaaatcagt atgaatcttc
     1501 gttaattcaa aaagtggagg acatgcaaaa tgaacttcaa taaattaaag tttggtgcga
     1561 ctattggcat tattggtggt ggtcagcttg gaaagatgat ggcacaatca gctcaaaaaa
     1621 tgggttataa agtggttgta ttggatcctt ctgaagattg tccatgtaga tacgttgcac
     1681 acgaatttat acaagccaag tatgacgatg aaaaggcact caatcaatta ggacaaaaat
     1741 gtgatgtgat tacttatgaa tttgaaaaca tttcagccca acaattaaaa ctattatgtg
     1801 aaaagtacaa tattccgcaa ggttaccaag ctatacagtt attacaagat cgcttaactg
     1861 aaaaagaaac attaaaaagt gctggtacca aagttgtccc gttcatttca gtaaaagaat
     1921 ctacagatat tgacaaagca attgaaacat taggatatcc ttttattgta aaaactagat
     1981 ttggtggcta cgatggcaaa ggtcaagttt taattaacaa cgaaaaagac ttacaagaag
     2041 gttttaaatt aattgaaact agtgaatgcg tagctgaaaa atatttgaat atcaagaaag
     2101 aagtatctct tactgttaca agaggaaaca acaatcaaat cacttttttc ccattacaag
     2161 aaaatgagca tagaaatcaa atacttttca aaacaattgt tccagcgaga atagataaaa
     2221 cagctgaggc gaaagagcaa gttaataaaa ttatccaatc gattcatttc attggaacat
     2281 ttacagttga attttttata gatagtaaca accaattgta tgtgaacgag atagcaccaa
     2341 ggcctcacaa ttccggacat tattcaattg aagcatgtga ttattcacaa tttgatactc
     2401 atattttagc agttaccgga caatcattac caaattcaat tgaattatta aagccagcag
     2461 tcatgatgaa cttactaggt aaagatttag atttattgga aaatgaattt aatgaacatc
     2521 cagagtggca cttacatatt tatggtaagt ctgagcgtaa agatagcaga aaaatggggc
     2581 atatgactgt actaacgaat gatgtaaacc aaactgaaca agatatgtac gctaaatttg
     2641 aggggagtaa ttaaagcatg acattattat atgaaggaaa agcgaagcgc attttctcaa
     2701 caaatcaaga aaatgaatta agagttgaat ataaagatga agttactgct ggaaacgggg
     2761 ctaagaaaga cacaatggca ggtaaggggc gattaaataa tcaaattact tctattatat
     2821 ttaaatattt acaagaaaat ggaatagaaa gtcactttat taaacaatta tctgaaacag
     2881 aacaattagt taagcctgtg aaaataattc cattagaagt agttgttcgt aatattgcta
     2941 gtggatctat tacaaagcgt ttaggttttg aaaatggtga agtttttaga gaaccacttg
     3001 tagaattttt ctataaaaat gatgcgttaa atgatccgtt gataacggat gaccatgtta
     3061 aattgctcaa tatagcatca gatgaagata ttgaaatact aaaatccaaa gcattaaaga
     3121 ttaataatgt gttgaaacaa ttaatggatg ctatgaattt aaaattagta gattttaaaa
     3181 tcgaatttgg aaagactgag actggtcaaa ttttgttagc ggatgaaata tctccagata
     3241 catgtcgaat ttgggataaa gctaccaatg caaactttga taaagatgta tatagaaata
     3301 acactggatc actgattgaa acatatcaaa tatttttaaa caaattggag gatttaaaat
     3361 aatgaaaaca attgaactac atatcacatt acaaccacaa gtattagata cgcaaggaca
     3421 aacgcttact cgagctgtac atgacttagg ttatgcacaa gtgaatgata ttcgtgtagg
     3481 aaaagtatta tatatgacag tggatgaggt tagtgatgaa aaggtacaca acattattac
     3541 aactctaagt gaaaaattgt ttgcaaatac agtgattgaa gaatatagct ataaagtgtt
     3601 agatgatgaa aaggagaatg cataaaatga aatttgcggt tcttgttttt ccaggttcga
     3661 attgtgatag agacatgttt aatgctgcta ttaaaagtgg tgttgaagcg gaatatgtag
     3721 attatagaga aacatcacta agtggatttg atggcgtact tattcctggt ggattttcat
     3781 tcggggatta cttaagatct ggggcaatgg ctagtgtagc gccgattatt tcggaagtta
     3841 aacgtcttgc agctgaaggt aagccagtat taggtgtttg taatgggttt caaattttaa
     3901 ctgaaatagg cttattacct ggtgcattat tgcataacga ttcacattta tttattagta
     3961 gaaatgaaga gttagaaata gtgaataatc aaacggcatt tacaaatctt tatgaacaag
     4021 gtgaaaaagt tatatatcct gtagctcacg gtgaaggtca ttattattgt actgatgaaa
     4081 tatatcaaca attaaaagct aacaatcaaa ttattctgaa atatgtgaat aatccgaacg
     4141 gttcatatga tgatattgca ggaattgtta acgaaaaagg caatgtatgt ggcatgatgc
     4201 cacatcctga aagagcttta gaaacgttgt taggtactga tagtggtgtg aaattatttg
     4261 aagcgatggt aaaaagttgg agggaacaac atgtctaaat ttatcgaacc aagcgttgaa
     4321 gaaattaaac ttgaaaaagt atatcaagat atgggattaa gtgatcaaga atatgaaaaa
     4381 gtttgcgata ttttaggcag acaacctaac tttacagaaa caggtatctt ttctgttatg
     4441 tggagtgaac attgctctta taaacattct aaaccgtttt taaagcaatt tcctacgtca
     4501 ggtgaccatg tgcttatggg gcctggtgaa ggtgcagggg tagtcgatat aggtgataat
     4561 caagccgtag tatttaaagt agagtctcac aatcatccat cagcaattga accatatcaa
     4621 ggggctgcta caggcgttgg tggaatcatt cgtgacattg tctctattgg ggctagacct
     4681 attaatttgt taaacagtct tagatttgga gaattagata ataaacaaaa ccaaagatta
     4741 cttaaaggtg ttgtaaaggg tatcggaggt tatggtaact gcattggtat tccaacaact
     4801 gctggtgaaa tcgaatttga tgaacgttat gatggcaatc cacttgttaa tgcaatgtgt
     4861 gttggtgtta tcaatcacga catgattcaa aaaggcacag caaaaggtgt aggtaattcg
     4921 gtcatttatg ttggtttgaa aactggtcga gatggtattc atggtgctac ttttgcatct
     4981 gaagaattga cggaagaaag cgaaagtaaa cgaccttctg tacaaatcgg tgatccattt
     5041 gtaggtaaaa aattaatgga agcaacactt gaagcaatta catttgatga attagttggt
     5101 attcaagata tgggtgctgc tggtttaaca tcttcatcgt ctgaaatggc ggcaaaaggt
     5161 ggtagtgggt tacatttgag attagaacaa gtgccaacac gtgagccagg tatttctcct
     5221 tatgaaatga tgctttcaga aactcaagaa cgtatgttac tagttgttga aaaaggtact
     5281 gaacaaaaat tcttagattt atttgataag cacgaattgg atagtgctgt tataggtgaa
     5341 gttacagata caaatcgttt tgttttaaca tatgatgacg aagtttatgc tgacattcca
     5401 gttgaaccac tagctgatga agcacctgta tatattttag aaggagaaga aaaagattat
     5461 aatacttcta aaaatgatta tacacacatc gatgttaaag atactttctt taaattactt
     5521 aagcatccga ctatagcatc taaacactat ttatatgatc aatacgacca acaagttggt
     5581 gccaatacga taattaagcc aggacttcaa gcatcggtag tacgtgtgga aggcacaaat
     5641 aaggcaattg cttcaacaat tgatggtgaa gcgcgttatg tatataacaa tccatatgaa
     5701 ggtggaaaga tggtagtagc tgaagcttat cgaaatttaa ttgccgtggg tgcaacacca
     5761 ttagcaatga cagattgttt aaattatggt tctcctgaaa agaaagaaat ctatcaacag
     5821 ttgatagatt caacgaaagg tatggcagaa gcatgcgaca ttcttaagac accagtagtt
     5881 tctggtaata tatctttata taacgaaacg aaaggtactt ctattttccc aacaccagtt
     5941 gttggaatgg taggtttgat tgaaaatgta aattatttaa atgattttga acctcaagtt
     6001 ggagataaat tatatttaat cggtgatact aaggacgact ttggtggtag tcaacttgaa
     6061 aagttaattt atggcaaagt taatcatgaa tttgagtcat tagatttgag ttcagaagtt
     6121 gaaaaaggtg aatcaatcaa gaccgctatt cgtgaaggac tattatcaca tgttcaaaca
     6181 gttggtaaag gtggcttact gattacctta gctaaactaa gtgcgcatta cggtttagga
     6241 ttaaaatctt caatagatat aacaaatgca caattgttta gtgagacgca aggccgatat
     6301 gttgtttctg ttaaatcagg taaaacttta aatattgata atgcaataga aattggactt
     6361 ttaacagata gtgataattt caaggtaaca acaccatata cagagattag tgaaaatgtt
     6421 tcagatatta aacaaatatg ggaaggggca attgctcaat gtttaactac tcaggattaa
     6481 acgaagaatg tggcgtgttt ggtatttgga atcatcctga agcagcgcaa ctaacatata
     6541 tgggacttca tagtttgcaa catcgtggtc aagaaggtgc aggtatagtt gtttctgatc
     6601 aaaatgaatt aaaaggcgag cgaggattag gcttactaac tgaagcgatt aaagatgatc
     6661 aaatggaacg attaaaagga tatcaacatg caattggtca cgtccgttat gctacttcag
     6721 gtaataaagg tattgaaaat attcaaccgt ttctgtatca cttttatgat atgagtgtag
     6781 gtatttgtca taatggtaac ctcattaatg ctaaatcatt gcgtcagaat ttagaaaaac
     6841 aaggtgctat cttccattcg tcttctgata ctgaagtcat tatgcatttg atacgtcgaa
     6901 gtaaagctcc tacttttgag gaagcgttga aagaaagttt gcgaaaagtt aaaggcggtt
     6961 ttacatttgc gattttaact aaagatgcgt tatatggcgc agtagatcca aatgctatca
     7021 gaccacttgt tgtaggtaaa atgaaagatg ggacatacat ccttgcaagt gaaacatgtg
     7081 caatagatgt gttaggtgca gaatttgttc aagatattca tgcaggtgaa tatgtcgtga
     7141 ttaacgataa aggtattaca gttaaatctt atacacatca tacgacaact gcaatttctg
     7201 cgatggaata tatttatttt gctagaccag actcaacaat agctggtaaa aatgtccatg
     7261 cagtacgtaa agcttctggt aaaaaattag cccaagaaag ccctgtaaat gctgatatgg
     7321 tcatcggtgt acccaattca tcgctatcag ctgcgagtgg ttatgctgaa gaaataggtt
     7381 tgccatatga aatgggacta gttaaaaatc aatatgttgc aagaacattt attcaaccaa
     7441 ctcaagaatt acgtgagcaa ggtgtgagag tgaagttatc tgcggtaaaa gatatagtag
     7501 atgggaaaaa tatcattctt gttgatgatt ccattgttcg cggtacgaca attcgacgca
     7561 ttgtgaaaat gttaaaagat tctggtgcaa ataaagtgca tgtgcgtata gcatcaccgg
     7621 aatttatgtt tccaagtttt tacggaatcg atgtttcaac tacggcagaa ttaatttctg
     7681 caagcaaatc acctgaagaa attaaagatt atattggcgc tgattcatta gcatatctat
     7741 ctgtagatgg gttaattgaa tcaattggtt tagattatga cgcgccatat agtggcttat
     7801 gtgtagaaag tttcactgga gattatcctg cagggttata tgattatgaa gcaaattata
     7861 aagcgcattt aagtcatcga caaaagcaat atatttctaa aaacaaacac ttttttgata
     7921 gcgagggaaa tttaaatgtc taaagcatat gaacaatctg gtgtaaatat tcatgctggt
     7981 tatgaagctg tagaaagaat gtctagtcat gttaaacgta cgatgcgtaa agaagttatc
     8041 ggtggtttag gtggattcgg tgctacattt gatttatcac aattaaatat gacagcgcca
     8101 gttttagttt ctggaacaga cggagtaggt acgaaattaa aactagctat cgactatggt
     8161 aaacatgatt cgataggtat cgatgcagtc gcaatgtgtg ttaatgatat tttaacgaca
     8221 ggtgcagaac cattatattt tttagattac attgctacaa acaaagttgt tcctgaagtt
     8281 attgaacaaa ttgttaaagg tattagtgat gcatgtgttg aaacgaatac tgcacttatc
     8341 ggtggagaga ctgctgaaat gggtgaaatg tatcacgaag gcgaatatga tgtagctgga
     8401 tttgctgttg gagcagttga aaaggatgac tatgtagatg gttcagaagt gaaagaggga
     8461 caagttgtta tagggcttgc gtcaagtggc attcattcaa atggatacag tttagtgcgc
     8521 aaattaatta atgaatcagg cattgatttg gcatcaaact tcgataatcg tccatttatc
     8581 gatgtctttt tagaaccaac taaattatat gtcaaacctg tacttgcttt gaaaaaagaa
     8641 gtttctatta aggcaatgaa tcatattact ggtggaggtt tttatgaaaa tattccacgt
     8701 gcattgccag ccggatatgc tgctagaatt gatactacat catttccaac accaaaaata
     8761 tttgattggt tacaacaaca aggcaatata gacacaaatg aaatgtataa catttttaat
     8821 atgggtattg gctatacggt tatcgttgat gaaaaagatg tatcacgcgc tttgaagatt
     8881 ttagcagaac aaaatgtgga agcctatcaa attggtcata ttgtgaaaaa tgagtcaact
     8941 gcaattgaat tgttgggggt ataacaatgg ttaaaattgc gatttttgca tcaggttcag
     9001 gaagtaactt tgaaaatata gttgagcatg ttgaatcagg aaaacttgaa aatattgaag
     9061 ttacggcgct atatacggat catcaaaatg cgttttgtat agatagagca aaaaagcacg
     9121 atattcctgt ttatattaat gaaccaaaac aatttgattc aaaagcagcg tatgaacaac
     9181 atttagtaac attattaaat gaagataagg tagagtggat tattctagct ggctacatgc
     9241 gtctaattgg tccagactta ttagcttcat ttgaaggtaa aatattgaat atacatccat
     9301 ctctattgcc gaaatataag gggattgacg caataggcca agcatatcat agtggcgata
     9361 ctattactgg ttcgacagta cattatgttg atagtggtat ggatacggga gaaattattg
     9421 aacagagaaa atgtgatatt agaccggacg attcaaaaga acaattagaa gagaaagtaa
     9481 aaaaattgga atatgagtta tatccaagtg ttattgctaa aattgtaaaa taaggagtat
     9541 gtatgtgatg aagaaagcta ttttgagcgt atcaaataaa acaggtattg tagagtttgc
     9601 taaagcgtta acgcaattaa attatgaatt atattcaaca ggtggtacta aacgtatatt
     9661 agatgaagca aatgtaccag ttcgttctgt ttcagactta acacattttc cagaaataat
     9721 ggatggccgt gttaaaacat tacatccggc tgttcatggt ggcattttag ctgatcgaaa
     9781 taaaccgcag catttaaatg aattatcaga acaacatata gatttaattg atatggtagt
     9841 agttaattta tatccattcc aacaaactgt tgcaaaccct gatgtgacga tggacgaagc
     9901 aattgaaaat attgatattg gtggtccaac aatgttacgt gctgcagcta aaaactataa
     9961 acatgtaaca acaattgtac atccggcaga ttatcaagaa gtattgacgc gattaagaaa
    10021 cgattcgtta gatgagtcat atagacaatc attaatgatt aaagtttttg agcatactgc
    10081 agaatatgat gaagcgattg tacgtttctt taaaggggat aaagaaactt taagatatgg
    10141 agaaaatcca caacaatcag cgtattttgt gagaacttcg aatgctaagc acacgattgc
    10201 aggcgctaaa caattacatg ggaaacaatt aagctataac aatattaaag atgcagatgc
    10261 tacactagct ttagttaaaa agtttgatac acctgctaca gttgcggtta aacacatgaa
    10321 tccatgtggt gttggtatcg gtgacacgat agaacaagca tttcaacatg cgtatgaagc
    10381 ggatagtcaa tcaatatttg gtggaattgt tgcattaaac cgagctgtaa cacctgagtt
    10441 agcagagcaa ttgcatagta tctttttgga agtcattatt gcaccaaaat ttacagatga
    10501 agcattagat attttaaaac aaaagaagaa tgtaagatta ttagaaattg atatgactat
    10561 agacagtaac gaagaagagt ttgtttcagt atctggcgga tatttagttc aagataaaga
    10621 caattatgtc gtgccaaaag aagaaatgaa agttgttaca gaagtagcac ctacagatga
    10681 acaatgggaa gcaatgttat taggatggaa agttgtacca tcagtaaaaa gtaatgcaat
    10741 tattttaagt aataataaac aaactgtagg tataggtgct ggacaaatga atcgtgtcgg
    10801 tgctgctaaa attgcgttag agagagctat tgaaatcaat gatcatgtag cgttagtatc
    10861 tgatggattt ttccctatgg gagatacagt tgaacttgca gcacaacatg gtataaaggc
    10921 aattatccaa ccgagtggtt cgattaaaga tcaagattca attgatatgg ctaataaaca
    10981 tggtattgca atggtagtca caggcactcg acattttaaa cactaatttt aaaggggcgt
    11041 aacataaatg aatgtattag taattggtgc tggtggacga gaacatgcac ttgcatataa
    11101 acttaatcaa tcgaatctag ttaaacaagt gtttgtcatt ccaggtaatg aggcaatgac
    11161 acctatagct gaagtacaca ctgaaatttc agaatctgat catcaagcga tactagattt
    11221 tgctaaacgg caaaatgttg attgggtagt tataggtcca gaacagccgc taattgatgg
    11281 attagcagac attttacgag cgaatggttt caaagtgttt ggtccaaata agcaagcagc
    11341 tcaaatcgaa ggctcaaaat tatttgctaa aaagataatg gaaaaatata atattccaac
    11401 tgctgattat aaagaagttg agcgaaaaaa ggatgcttta acatatattg aaaactgtga
    11461 attgcccgtt gttgtcaaga aagatgggtt agctgctggg aaaggcgtta ttattgcaga
    11521 tactattgaa gcagccagaa gtgctattga gattatgtat ggtgatgaag aagaaggtac
    11581 tgttgtattt gaaacgtttt tagaaggtga agagttctcg ctaatgacat ttgttaatgg
    11641 tgatttagca gtacctttcg actgtattgc acaagatcat aaacgcgcat ttgatcatga
    11701 tgaaggacca aatactggtg gtatgggggc ttattgtcca gtaccacata ttagtgacga
    11761 tgttttaaaa cttacaaatg aaacaattgc acaacccatt gcaaaggcaa tgcttaatga
    11821 aggttatcaa ttcttcggtg tattatacat tggtgctatt ttaactaaag atggtccaaa
    11881 agtaatagaa tttaatgccc gttttggtga tcctgaagct caagtattat taagtcgcat
    11941 ggaaagtgat ttaatgcagc atattattga tttagatgaa ggaaaacgta ctgaattcaa
    12001 atggaaaaat gaatctattg taggggtcat gttggcatca aaaggatatc ctgatgcata
    12061 tgaaaaaggg cataaagtaa gtggctttga tttaaatgaa aactattttg ttagtggatt
    12121 aaagaagcaa ggtgatacct ttgttacttc aggtggtaga gttatacttg ccatcggaaa
    12181 aggtgacaat gtacaagatg cacagcgaga cgcatacaaa aaagtatcac aaatacaaag
    12241 tgaccattta ttctatcgtc atgacattgc gaataaagca ctacaactta aataagtaaa
    12301 tttaaaatac taagattagc tatgaacgaa tctataacga tagatttttt catagctttt
    12361 ttagttgtag agtctaggac attgatttct gtaccaaatt tgtgattatg catatgtaat
    12421 acaaaagagg cgccacaaca tgtttggatg aacaaaataa catgtttgtg gcacctcttt
    12481 tgtttagtat ggaataaatg gttttctttt tctatacaat gaatttctaa tttagtatct
    12541 atacaattat ggataaaatt taacctacac gaccaagacg aacatcatct atgcccgtga
    12601 tgggtaaggt gattgaacaa taatatgcca tagtaataat ggcaattaaa actataataa
    12661 agattatatc tttatatgag aaaggtacgt tgtaatagta agtacgagga ccatctctaa
    12721 atcctttcga ctccatcgca actgataatt gatgtgcctt tctaatattt tggcttaata
    12781 gaggtataat taaatgctta aatcgcttta accctctata atttgccgcg tctatcatct
    12841 gatagcgcat ttttaaagat ctgcgaagct gtattaaaga actaatcatt aaaggtatca
    12901 tacgaatggc agccatgaat gcataagcaa cttttgattt aacctttaaa tgttgcatta
    12961 aactataaaa tatcatgaca acctgagatg taagtgcgat taagataccg aaaaatgaaa
    13021 tagcaatggt tcttaatgat acatgtaaac cacgaactaa actttctgtt gtaatatgga
    13081 taaatccgaa tttcaaaatt gtatggctac cattcccgta taaaatcatg aacagggaag
    13141 agagtaatgc aaagccaata cttatagtta taaaaattgc tgtaatttta aactgagtac
    13201 cattaaacat caataagaaa actaacatta agatagtgat ataaagcata aaatcgaaat
    13261 tatgcacaaa tataataaag aaaaatagta taattccaag aaatagtttc gttataatgt
    13321 tgacatcatc aacaaatgat tgccgaactt tccattgctc atacattcgt atcaccatca
    13381 caatctagta acgcaccatc tgaaatttta agtcttcttg atggataacg ttcaattatt
    13441 tcatcgtcat gtgtaaccat gacaatactt tgtcccaaat taattcgctt ttggaaaagt
    13501 ttgatcaact ggaatgtatt atggctatca agtccaaatg tcggttcatc taaaaagata
    13561 atatcagctt tagaacttag tgcggtagct acgctaaggc gtcgtttttg accaatagac
    13621 aactcataag gatgttgatc ttttacattt tgtaaatcta aaagttttaa aagttgtatc
    13681 gtttcatcat cactttgatc tttagaaagg tgattaaaat gaatgttaat ttcatcataa
    13741 accgaatttg ttataaattg taattctggg ttttgataaa ctaggtacat gtgttttgct
    13801 gcatgtttaa tttttgttaa acgctgattt tcaaaataaa catcaccttg atatttaatc
    13861 aattgcataa ttgattcaag caaggttgtt ttaccactac catttgcccc tgtaattgta
    13921 atccactcac ctagaccaat ttctaaatct gagaatgaga gcaatgttga tttaccgcga
    13981 ataatacgtc catttttaaa ttgtaataag tgtgagtttg ttgttggaaa gtcaacacga
    14041 cttggtgcga attcccatgc acgtggatgc cacacaccat attcactgag taaatgaaca
    14101 tacttctgta atatgatttc aggacattca tcggcaatga tatttccgtt ataatccatc
    14161 aaaatgacgc ggtcgacatg attccagatg tgtttaactt tatgttcaac gattacaacc
    14221 gtttgatctt cccaaagttc aattagttta gtccataaat cttctgttgc ttgaacatct
    14281 aacattgctg tcggttcatc taaaaacaat gtttttgatt gttgaagaat ggtttcaaca
    14341 attgccaatt tctgtttcat cccgccactt aaatctttga tatacgtttc aggggtaaca
    14401 tttaaattga ccatatttaa agcattgata attaacgcat ccatgtcttc acgtggtaat
    14461 tgtctatttt ctaaaacgaa tgcaagttct tcgtatactt ttggcataca aaactggcta
    14521 tcagggtctt ggaaaataac gccacttaat gggtcaacga ttagttcatc atatttcata
    14581 ggtaattcaa ttaaattagg aacaatacca cttaatacat tcagaagtgt acttttaccg
    14641 caaccagaag gaccgagtaa aagtactttt tctttgtctt gaatagtgat atttaaatga
    14701 tcgaaaattt tacgttgacc acttggatat tttaatcgta aatcacttac ttttaacact
    14761 ctgaatgctc ctcttataag ttgtcgtaat cttctttagc agctggtcta aataattttg
    14821 ttacgcctgt cttatctaaa gcttttacta aaaggtaaga taggacgccg gcgactactg
    14881 caccactaat taatctaaat acgatgaata atgttaagtt ccaacctgca acttcattta
    14941 aataaccata gaaataatct atcgggaaag ccgcgattgc tgtacaaaaa cctgctaaca
    15001 tagctaccat aactgaacgt gattgatatt taaaaattgc aaagacaagt tcacacgcta
    15061 aaccttgtat aaaagcgtaa acgattgtcg gaatatcgaa acgacccata atgatagttt
    15121 cgccggcacc tgcagcaaat tcagccagta aagcaatacc tggttttgga ataattagat
    15181 agcagacaat cgctgccatg aaccaaaccc cgtttgttaa ttgttcgagg tgaaggcctg
    15241 tagcttgcac accattgtaa acaaaccacc ataaattgta aataactgcg aatactactg
    15301 aaataagtac ggttactagt atttcagata gctttaaacc ttttgacatt tttacatcct
    15361 cctaataaaa aaacgcacaa ccatccatag gaaagttatg cgttcacaat atatattagt
    15421 aaaacatatg tatagtaaca ctttcctacg ctagttcaag ctagatcagg ttcaaagggt
    15481 ttgagggcaa gcctcatctc agtataaaac acccctagtg tgtgcgattt atttaattaa
    15541 ttatactgta agacgtttgt aaacttatgt caataggttg tcttcatgaa atttcgttta
    15601 attcgattta aaatttataa tattagcatt ggatttaaat tgaagatgta gtaggaatgt
    15661 tagtaattaa agatataaaa atatgtgaca tgtaataata ttgagctgat aaatgaagag
    15721 ggatacttat caatcatacc tctttaacaa cagtgaagaa cccgtgcata atggcttacg
    15781 aattatagtt tataaggaag aagagggata catgcgccga gcacatgcat aaaagcccct
    15841 aacaactaaa agttgtaagg aaggagaggg atacatgcgc cgagcacatg cataaaatcc
    15901 cctaacaact aaaagttgta aggggattta aattaattta gtgtatcttg gatatcttgt
    15961 tttgtttgat taatatcttc tgttttttct tcttttttat cttttaattt ttcttcaact
    16021 tctttagctt tttctgctgc ttttttattt tgattttcat tagacatgat taattcctcc
    16081 caaattggat aattatttat atataaatct tacccggttg tactttcgtt aaacttttct
    16141 aagtctatag cactatttat tcatttatct aaagacaaca acattagatt aatatataat
    16201 gattttgagg tgaacataat gtcttttctt aggaaacacg ccgaaattat ttttagctat
    16261 ttaatcggta tcgtttcact cttcactggt ctcattattt taattaactt gccattaatt
    16321 aaacaattaa atggtggtaa aaaagttgat acacatgctc ataatgtgtg ggaatttctg
    16381 aatgcatttt tcagtgaaat tattaaagta atgagtcgat ttataggtaa tttccctata
    16441 gttagtgcaa ttgtgataat tatattcggt attttagtta tgttgattgg tcatacatta
    16501 cttagaacta ttaagtatga ctatgatatt tctatctttt tcttagttat cggtatcatg
    16561 tactttatta ttactcttat attaatgact caagtttatg gattctttgc agtgattttc
    16621 attattccat ttacaattca tataggatat atcgtctata aagatgaatt gaatcaggaa
    16681 aatgtaaaaa atcatttcat gtggataatt gtgagttatg gtataagtta cttaattaca
    16741 caaattgcat tgtatggcag aattgatgct aatgaaatag agtcaattga tatcttaagt
    16801 gtcaatgctt tctttataat tatgtggtta cttggtcaaa tggctatttg gaatttcttg
    16861 ttcttgcgcc gagctttacc tttaacaaag caagaattag gtgaagagga gccagaatta
    16921 tcaagaacaa gtaaagggaa tgtcacgaat caaactaaaa ttcacttgaa acaactccaa
    16981 gataagacta cagaatatgc acgtaagaca agaagaagtg tcgatttaga taaaattaga
    17041 gctaaaagag ataaattcaa aaagaaagtt aatgatatta tcgatattca agaagacgat
    17101 attcctgatt ggatgagaaa accgaaatgg gttaaaccaa tgtatgtcga actattttgt
    17161 ggtgtcgtca tctttttatt cacattttta gaatttaata atcgtaatgc attatttgta
    17221 tctggtgatt ggaaattatc acagacacaa tatgttattg aatgggttac attattaatt
    17281 ctgttattca ttattatcgc atatatcgct acaacgttaa ctttccactt gaaaggtaag
    17341 ttttattatt tacaattatt tatggggagc attttattct ttaaattgtt aacggaattt
    17401 ataaatataa tgattcatgg actattactt tcagtgttca ttacgccaac attactatta
    17461 atgttattgg caatcatcat ttcttattcg ttacaattac gagagcgacc ataattaaaa
    17521 gcattataaa agtactatct attaaacatt ttgatgtgta cgctataagt tagatatatc
    17581 tctaacttac ttagatacag gtcaatgaag tttatggata gtactttttt tgtaactaga
    17641 tttgattgat tcaggtgatg tgaattaagt attgaaaatt gtatacaaag ttttagtgca
    17701 aataaaatag ttgaaaagtt atccatttgt aaaatcaaga aaactagtaa atagttgaag
    17761 cgacttatgg aatttgcgaa acgatatata gtatttcctt tgtagaaatt taacatatat
    17821 cattcgaatt actaatttgt taaaatcaac agtaagatta gaagtagatg atattgaaat
    17881 ttggcaaaca atttaatcta tataaaacta caacgaaaca cagaaaggaa gttgtcagat
    17941 gaaaatagca actctgaaca aaggcaaaga aacaaaatat tttaatggat atcctttaat
    18001 tgaagaagag gatatctatt cacaagatca tttaaaagaa ggagatattt ttcaaattgt
    18061 gactgataaa tcacaatatg tcgcaacggc ttatgttggt cgccaacata aaggattagg
    18121 ttgggttcta acatacgata aagctcaaca aatcaacaca gctttctttg taaaattgtt
    18181 taatactgca ttagcagaac gtgattatta ttttaatata gatggaacaa atgcttttag
    18241 attatttaat gctgaaggtg atggtgttgg gggattaaca atcgacaatt acgatggtca
    18301 tttgttgatt caatggtact caaaaggtat ttataaattt aaatatgcca ttcttgaagc
    18361 ggttagaaaa gtatttgatt ataaatctat ttacgaaaaa gtaagattta aagacagcga
    18421 atatagtggt ggttttgttg aaggagatgc acctgagttt ccaattgtta tcgaagaaaa
    18481 cttcacattt tataatgtag accttgaaga tggtttgatg acaggtatct ttttagatca
    18541 aaaagaagtg cgcaagaaat taagggatca atatgccaaa gaacgccatg ttttaaactt
    18601 atttagttat acaggtgctt tttctgcaat agcagcaagt gaggcatctt caacaacaag
    18661 tgtagatttg gctaatcgtt ctcgtagttt aactgaagaa aattttggat taaatgctat
    18721 tgatcctaaa tcccaatata tttatgtcat ggacactttt gatttctata aatatgctgc
    18781 acgacatgga catagttatg acacgatcgt gattgatcca cctagctttg cgcgtaacaa
    18841 aaaacgtaca ttttcagtgc aaaaagatta tgacaaatta attaatggcg ccttaaatat
    18901 cttatcatct gaaggaacat tattgttatg tacaaacgca agtgtatatc cattaaagca
    18961 atttaaaaat actattaaaa agacgcttga agagagtggc gttgattatg aattaactga
    19021 agttatggga ttaccaaaag attttaaaac gcatccacat tataagccat ctaaatattt
    19081 aaaagctgtt tttgtaaata ttagacatta atttgataaa aaggggtata gtaacttatt
    19141 gagaaaaaga agggtgataa tattatggga ttcaaaaaca atttaacatc aaatttaaca
    19201 aataaaatcg gtaattcagt ctttaaaata gaaaatgttg acggaaaagg tgcaatgcca
    19261 acgacgattc aagaattgag agaaagacga caacgtgctg aagcaattgt aaagagaaag
    19321 tctttaatgt catcaacaat gagcgttgtt ccaattccgg gtttagattt tggtgttgat
    19381 ttaaaattaa tgaaagatat tatcgaagat gttaataaaa tttatggttt agatcataag
    19441 caagttaata gccttgggga tgatgtgaaa gaaagaatta tgtctgcagc agcaattcaa
    19501 ggtagtcaat ttattggtaa aagaatttca aatgcatttt taaaaattgt aattagagat
    19561 gtagctaaac gtactgctgc aaaacaaaca aaatggtttc ctgttgtagg acaagctgtg
    19621 tctgcatcta ttagttacta ttttatgaat aaaattggaa aagatcacat tcaaaaatgc
    19681 gaaaatgtta ttaaaaatgt catgtaggtg ctataatagt tttgcaattt gcaaatttta
    19741 ctgaaaccgg ttttaaacga attgaattta aagcatggtt ttggtaaagt taatgtataa
    19801 aactaagtta gtattgtaat aatattgaag attctaacta tacgaaggag aaatgtaatt
    19861 atggaacaaa attcatatgt aatcatcgac gagactggta ttcacgctag accagcaaca
    19921 atgttagtac aaacagcttc aaaattcgat tctgatattc aattagaata taacggtaag
    19981 aaagtaaact taaaatcaat catgggtgtt atgagccttg gtgttggtaa agatgctgaa
    20041 attacaattt atgctgacgg tagtgatgaa tctgacgcca ttcaagcaat cagtgacgtc
    20101 ttatcaaaag aaggattgac taaataatca tgtctaaatt aattaaaggt attgccgcat
    20161 ctgatggtgt cgcaattgct aaagcttatt tattagttga gccagactta acattcgaca
    20221 aaaatgaaaa agtcactgat gttgaaggag aagttgcaaa gttcaatagc gctatcgaag
    20281 cttctaaagt tgagttaact aaaattagaa ataatgcaga ggttcaacta ggtgctgata
    20341 aagctgctat ctttgatgca catttattag ttttagatga ccctgaatta attcaaccaa
    20401 tccaagataa gattaaaaat gaaaacgcta atgctgctac agcattaacg gatgtaacaa
    20461 cacaatttgt tacaattttt gaatctatgg ataacgaata catgaaagaa cgtgcggctg
    20521 atattcgcga cgtttctaaa cgtgtgttat cacatatttt aggtgtagaa ttaccgaatc
    20581 cgagtatgat tgatgaaagc gttgttattg tagggaatga cttaacgcca tctgatactg
    20641 ctcaattaaa taaagaattc gtacaaggtt ttgctacaaa cattggcgga agaacaagtc
    20701 actctgcaat tatgagtcgt tctttagaaa ttccagcaat tgttggtaca aaatcaatta
    20761 ctcaagaagt taaacaaggc gacatgatta tcgtagatgg attaaatggt gatgtaatcg
    20821 ttaatccaac tgaagatgag ttaatcgctt atcaagataa acgtgagcgt tattttgctg
    20881 acaagaaaga attacaaaaa ctacgtgatg ctgatactgt tacagttgat ggtgttcacg
    20941 cagagcttgc tgcaaatatt ggtacaccta atgatttgcc aggtgttatt gaaaatggtg
    21001 cacaaggtat cggcttatat agaactgagt ttttatatat gggtcgtgac caaatgccta
    21061 cagaagaaga acaatttgaa gcttataaag aagtattaga agcaatgggc ggtaaacgtg
    21121 ttgttgtacg tactttagat ataggtggag ataaagaatt atcatactta aacttgcctg
    21181 aagaaatgaa tccattctta ggttaccgtg cgattcgttt atgccttgcg caacaagata
    21241 ttttcagacc acagctacgt gcattattac gtgcatcagt ttatggtaag ttaaatatca
    21301 tgttcccaat ggttgcaaca attaacgaat ttagagaagc taaagctata ttattagaag
    21361 aaaaagaaaa ccttaaaaat gaaggtcatg acatttcgga tgatatagaa ttaggaatca
    21421 tggtagagat acctgcaaca gcagcattag ctgatgtctt tgctaaagaa gtagatttct
    21481 tcagtatcgg tacaaatgat ttaattcaat acacattagc tgctgaccgt atgtcagagc
    21541 gtgtatcata tctataccaa ccatataacc cttcaatctt acgtttagtt aaacaagtta
    21601 ttgaagcgtc acataaagaa ggtaaatgga caggtatgtg tggtgaaatg gctggagatg
    21661 aaacagctat tccattattg cttggtttag gtttagatga gttctctatg agtgcaacgt
    21721 ctattctgaa agcaagaaga caaattaatg gtttaagtaa aaatgaaatg actgaacttg
    21781 ctaaccgtgc agtcgactgt gcaacgcaag aagaagttat tgaattagtt aacaactacg
    21841 taaaataatt aattctttcg aaagacagaa ttataaataa acccgaagta ttcattgtaa
    21901 gtaattatga atacttcggg ttctttttta tgatttatgt taatttcaat ggaatgttgt
    21961 tgctaactaa ttaaacaact atgtgtcatt aacaaaatgt agttctttaa ttaatgttag
    22021 ttaatttaaa
//
LOCUS       DALAGF010000039        21339 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.039, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000039 DALAGF010000000
VERSION     DALAGF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21339
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.039"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(117..707)
                     /locus_tag="O2692_001353"
     CDS             complement(117..707)
                     /locus_tag="O2692_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCZ4298988.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            complement(861..2123)
                     /gene="clpX"
                     /locus_tag="O2692_001354"
     CDS             complement(861..2123)
                     /gene="clpX"
                     /locus_tag="O2692_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCZ4298989.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            complement(2274..3575)
                     /locus_tag="O2692_001355"
     CDS             complement(2274..3575)
                     /locus_tag="O2692_001355"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCZ4298990.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            complement(3623..3733)
                     /locus_tag="O2692_001356"
     CDS             complement(3623..3733)
                     /locus_tag="O2692_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298991.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            complement(3738..4667)
                     /locus_tag="O2692_001357"
     CDS             complement(3738..4667)
                     /locus_tag="O2692_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298992.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLADFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLK
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            complement(4686..5294)
                     /locus_tag="O2692_001358"
     CDS             complement(4686..5294)
                     /locus_tag="O2692_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCZ4298993.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            complement(5436..5792)
                     /gene="rplT"
                     /locus_tag="O2692_001359"
     CDS             complement(5436..5792)
                     /gene="rplT"
                     /locus_tag="O2692_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCZ4298994.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            complement(5839..6039)
                     /gene="rpmI"
                     /locus_tag="O2692_001360"
     CDS             complement(5839..6039)
                     /gene="rpmI"
                     /locus_tag="O2692_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCZ4298995.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            complement(6068..6595)
                     /gene="infC"
                     /locus_tag="O2692_001361"
     CDS             complement(6068..6595)
                     /gene="infC"
                     /locus_tag="O2692_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCZ4298996.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     misc_feature    complement(6624..6749)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(6824..8317)
                     /locus_tag="O2692_001362"
     CDS             complement(6824..8317)
                     /locus_tag="O2692_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ4298997.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     gene            complement(8743..10680)
                     /gene="thrS"
                     /locus_tag="O2692_001363"
     CDS             complement(8743..10680)
                     /gene="thrS"
                     /locus_tag="O2692_001363"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCZ4298998.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            complement(10878..11000)
                     /locus_tag="O2692_001364"
     CDS             complement(10878..11000)
                     /locus_tag="O2692_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4298999.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            complement(11093..12013)
                     /gene="dnaI"
                     /locus_tag="O2692_001365"
     CDS             complement(11093..12013)
                     /gene="dnaI"
                     /locus_tag="O2692_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCZ4299000.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            complement(12013..13413)
                     /locus_tag="O2692_001366"
     CDS             complement(12013..13413)
                     /locus_tag="O2692_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HCZ4299001.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSAGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            complement(13414..13884)
                     /gene="nrdR"
                     /locus_tag="O2692_001367"
     CDS             complement(13414..13884)
                     /gene="nrdR"
                     /locus_tag="O2692_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCZ4299002.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            complement(14094..15119)
                     /gene="gap"
                     /locus_tag="O2692_001368"
     CDS             complement(14094..15119)
                     /gene="gap"
                     /locus_tag="O2692_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCZ4299003.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            complement(15289..15912)
                     /locus_tag="O2692_001369"
     CDS             complement(15289..15912)
                     /locus_tag="O2692_001369"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCZ4299004.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            complement(15928..16800)
                     /gene="mutM"
                     /locus_tag="O2692_001370"
     CDS             complement(15928..16800)
                     /gene="mutM"
                     /locus_tag="O2692_001370"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCZ4299005.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            complement(16816..19446)
                     /gene="polA"
                     /locus_tag="O2692_001371"
     CDS             complement(16816..19446)
                     /gene="polA"
                     /locus_tag="O2692_001371"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCZ4299006.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            complement(19740..21227)
                     /locus_tag="O2692_001372"
     CDS             complement(19740..21227)
                     /locus_tag="O2692_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299007.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLCFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSMIAALIIYLLLR
                     SDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFVNVIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIHVIILIILL
                     KEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQEVYTV
                     ESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
BASE COUNT         6576 a         4033 c         2880 g         7850 t
ORIGIN      
        1 ttttgagttt ttgacaaaat gatgaacgtt gaaaaatgtt atcaattgtg tactaattaa
       61 aagtttcgtg cttacattat ttttaaaatt tttgtataag ttttgacgta caaaaactat
      121 gaaatatacg gttcaattaa attccatatt tgttgttgtt tattattttg aattgatgaa
      181 taacttacaa ttgtatcgtc tgggtccata tctaattgtg tcttaatatt tttaatatgc
      241 ttttgaacct taccttttgg aattttgtct tctttagtgc atataactaa agtaggaata
      301 tcaaaatgtt tcaaataatt gtacattaag atatcatctt gtgttggatc atgtcttaaa
      361 tcaactaatt gaataactaa ttgcaaattc tctctcttag ttatatattc ctcaatcatt
      421 ttcccaaatt tttcacgttg tgttttactt actttagcat atccataccc tggaacatcc
      481 acaaaaataa gttgttcatc tatattataa aaatttaacg tttgcgtttt gccgggttgc
      541 tgtgatgtac gtgccatatt ttttctgcca atcatactat tgataaatgt agacttacct
      601 acattagatc gaccgctcag tgcaacttca gacaattctg tttctggata ttgttcttct
      661 tttactgcac taatgattaa ttctatatta ttaggattaa ctttcatttt atatcctcac
      721 ttttttatat tctctttcaa ttctaccatt atataatatt tgaacctttc ggtatacatt
      781 ataaaaaagc tccgatcaaa gttaaactat ttgagcgtca actttgattg gagcttttca
      841 cttttataac acatcaatga ttaagctgat gttttactat tattaattaa attgccttct
      901 gcgtcgtata gttctggttc agtttcttca ttaattgttt gtgctgtaat aactaccttc
      961 gttacatttt cgttagaagg cacatcaaac ataatatcga ttaacgattc ttctatgatt
     1021 gaacgtaaac cacgcgcacc tgtttttctt tcaattgctt tttcactaat tgctgataaa
     1081 gcttcttcag tgaactctaa atccacatca tctaattcca gcattttagt atattgtttc
     1141 acaagtgcat ttttaggttg cgttaagatg tttttcaacg cagttacatc taatgtttct
     1201 aaattagcta caattggcac acgtccgata aattcaggaa tcaaaccata ggcttgcaaa
     1261 tcttctgggc gaatttgtgc taataatgct tgttcgtcat atttatcagc ttcattgctt
     1321 gagaaaccaa taactttttc accaagacgg cgcttaatca cttcttcaat accatcaaag
     1381 gcaccaccaa gaataaataa gatatttgtt gtatcaattt gaatcatttc ttggtttgga
     1441 tgtttgcgtc caccttgtgg cggaacactt gcagtcgtac cttctaagat tttaagcaat
     1501 gcttgttgaa caccttcacc tgaaacgtca cgtgttatag atgtgttttc agatttacgt
     1561 gcaattttat caatttcatc tacataaata ataccttttt cggctttatc aatgtcaaag
     1621 tcagctgctt gaattaatct caacaagata ttttcaacat catcgcctac ataaccagct
     1681 tcagttaaac ttgtcgcatc tgcaattgca aatggtacat tcaacgtctt ggctaaggtt
     1741 tgagctaata atgttttacc actacctgtt ggcccaatta atgcaatgtt acttttttgt
     1801 aattcaacat catcttcttt tggtcctaat tgttgaatac gcttatagtg gttataaaca
     1861 gctacagcta aagatttttt agctttttct tgaccaataa catattcgtt taaatgatcc
     1921 ataatttctt taggagtagg taattctgtc atcgcttcag aagtgttttg agctaattct
     1981 tcttcgacga tttctgagca taattcaata cactcattac aaatatatac accacttcct
     2041 gctacaagtt tttttacttg atcttggtct tttccgcaga aagagcattt caaattttct
     2101 tcatcttcat tgaatttaaa cattcttttt acacccctat tcgttaaaga ctatactaga
     2161 ttggatgtta caatgcaaca tattaacata caaacttttt gcttaaagaa tagtagcaga
     2221 tacataagct atgcatctgc tactcatttt tgtaatttaa tatttaatga agattaatct
     2281 tctttagttc cttcaacgaa ctttgcgtta tctcttaata aatcgataac tttttggata
     2341 cgaacatcat ttttaatgat atcagtatta cctaaagtat ttttgatatc ttcaactgag
     2401 atattaaatt gtttactcat tttttctaat tctttatcga tatcttcatc agtagcttcg
     2461 attttttcag cttcagcgat cgcagttaaa gttaagttag ttttaacacg ttgttctgca
     2521 tcgtctttca tttgctctct taattgagtt tcatcttgac ctgagatttg gaagtacgtt
     2581 tgtaaatcta aaccttgttg ttgaattctt tgtgcaaatt cagacaccat acgatctaat
     2641 tcagtattaa ccattgcttc aggaatatcg attgttgtat tatcagtagc ttttgtaatc
     2701 gcttcttctt tttcaacatt ttcagcatct gtagcttttt gttcagctaa acgtttacgt
     2761 aagttttctt tgtactcgtc tactgtattt gcttctgcat ctaattcatt agcaatttca
     2821 tctgttaatt ctgggacttc tttaaattta atttcgttaa cttttgtttt gaaagttgct
     2881 tctttaccgg ctaattcttc agcatggtat tcttctggga atgttacgac aacatctttt
     2941 tcttcgtcaa ctttcatacc ttctaattgc tcttcgaaac caggtatgaa tgaacctgaa
     3001 ccgatttcta aatcgtaacc ttcagcttgt ccaccttcga attcttctcc gtcaactgaa
     3061 ccactaaagt cgatgttaac tgtgtcgcca ttttcaacaa caccatcttc tttaacgacc
     3121 atttcagcta aatgtcctaa gctgtggtca atcgcttctt gtaactcatc atcagataat
     3181 tcagtttctt gtttttcaat ttcaagacct ttatagtctc ctaatttaac ttctggctca
     3241 actgtaactg ttgcttcaaa aatgaaatct ttaccttttt caatttgagt aacacttact
     3301 tctggttgtg caactggttt aatatcagtt tcgtcaattg cttcaccata agcatctggt
     3361 aataaaatgt cgatagcatc ttgatataat gcttctacac caaagcgttg ttcaaaaatt
     3421 ggacgtggca ctttaccttt acggaatcca ggtacgttaa tttgtttaac cacttttttg
     3481 aatgcttgat ctaacgcttt gtttactttt tctgcaggaa cagtaacagt taataaacct
     3541 tcgttacctt cctttttttc ccaagttgct gtcatgtata tatacctcca tgattaacta
     3601 atttattttt tcaacttccc tattatatca taggtctatt ccctatacaa acattgaaat
     3661 cacaacgttt atatatttgt aaatcaactt ttttcgtcaa aactatatta aaatgctgag
     3721 tattattttg caacaattta actattatta ttcaaatcta attgctgaat aaaattaaat
     3781 gcatcgatac tttgtaattc acatttaagt cctaacatat ttttgaaata acattcgtat
     3841 gcattaatcc atttattcgt ttcaaacaaa gtttcaatat caattggata aatcatgata
     3901 gagtgattat tcataatatg gtgtgcttct tcagcgatat gtaaagcacc atcatttaat
     3961 gtttccataa cgttaggtat aactttttct ttaagtgttg tatgttctag cccttttaaa
     4021 ttagctggta caaaagttac atccatacca tacttttcaa ttgtcagttc ttgtgtacaa
     4081 tttgcgaacc ttaaatactc aatcattaaa ctaatgaggt tataactgta cgtattagat
     4141 tttaatatat ataataccgt ttcttgaaat tgaaaatgac cattgtctat taacttcaaa
     4201 atcaagtgcg tctgttccct cattgataac gtatcaaaat cagctaatga ctgagtcaat
     4261 cgtttttcat cttcaattaa ttttgactta gcaaattctt taagaggatg taatgccatt
     4321 cttgttttgt gatctttgac ttcatcaata atttgatgaa ttacttctac cgcttcaaaa
     4381 tattgtccca aaccaatcaa acttttcacg taataaatca tcaatgcatc atattgttgt
     4441 atgccagttt tcaataagac aattgtttct tctcttaatt caagaaatga ccccgtctca
     4501 tataacatac ggcatttcat cattgctatt tcttcagtga gttcaaattg acgttcatat
     4561 tgcacaatat attcatacat caggtcatag ttttgatctt gttcagcacg ttttatatct
     4621 ttatataact ttttcgaaga gttaggaaac tgaataatat ctgacatata tgtcacctcg
     4681 tttgattata aatcttgaag gattaatgca ctccacgttt cgaaatcatc atatgactct
     4741 atgttgcctt tttcaaccca ttttattcgt aaagtatctg tttctttagc ttctacatca
     4801 ttcgtactta cagtgatttt aaatacaaca ccaatatgta ccttgcccac ttcattatta
     4861 tcgtcattaa taaaaccgat atattccata ttttgtgaat cttgctcact taaacctact
     4921 tcttcttcta attctctctg tgcattaact ctcaatactt cgttaataga ttctgctcct
     4981 ggaacatcat tcatatgacc gcctacacct attgaagatt gtccatgcaa tcgagcttct
     5041 ccaccgccag ataatcgttc atacactaat atctcgccat gctcattttc aagtaaacaa
     5101 taagaaatga gttgtttata tgatggatcc tcttccatat cgccgcgtcg cttaacttca
     5161 tattgactta gcgcatcaaa aatattttga ccttctggtt tattcttatt taaaaaccca
     5221 ttgaaagtat tcttttcatt attaaaaata atttctctag gtactacaat gatttgttca
     5281 tcaaatttag acatcgtata aaactcctca ctttaatgtt tgttctctta aatgcatttt
     5341 agcaaatatg tcatatttta gcgattaaat gtggcatttt gctatttaaa aaataaaaaa
     5401 gagatgacaa tgccatctct tttttataag tgttattatt ttaaagcatc tttagcttta
     5461 gttactaatt gagcaaatgc tttttcgtca gaaattgcga tttctgataa cattttacgg
     5521 ttaatgtcga taccagcttt tttcaaaccg ttcattaaac gtgagtagct catttcatgt
     5581 tgacgagctg ctgcgttgat acgtgtaatc cataatttac ggaagtcacg tttacgttga
     5641 cgacggtcac ggaaagcata ttgacctgat ttcattactt gttgcttagc tactttgtat
     5701 aatgtatgtt ttgaaccgaa gtaaccttta gctaatttaa tcgttttttt acgacgcgct
     5761 cttgttactg ttccaccttt aactcgtggc ataaaaaatt cctccttagg taattcctac
     5821 cgatttctgt atttgttctt attttttgta tgctaataat tgttttacac gtttcatatc
     5881 gctcttagac actaatctag ctttacgtaa ttgacgtttt tgtttagtgc tcttgtttgc
     5941 gaataagtga gatgtgaaag ctcttgaacg ttttaattga cctgaagcag ttcttttaac
     6001 acgtttagct gctccgcggt gagttttcat ttttggcata atataagtcc tcctgtaata
     6061 gctttaatta tttttcagct gttggcgcta acatgataaa catttgacgc ccgtccattt
     6121 taggtttttg ttcaactgtt gctatatctt tgcattcatc tgcatatttt tctagcacac
     6181 gttgaccaat ttccttatgc gtaatggcac gccctctgaa acgaatagat actttacatt
     6241 tatcgccttt agttaagaat ttacgtccgt ttttcaactt cgtttggaaa tcatgttcct
     6301 caattgttgg acttaaacga atttctttaa cattgataat tttttgtttc tttttcattt
     6361 ctttttcttt tttctgttgt tcgaatttga atttaccgta atccataatt cttgcaactg
     6421 gtggtttcgc attcggtgca acgaccacta agtctaaatc tacacgttca gccatttcta
     6481 aagcttcacg ctttgattta acaccaattt gttcaccatc ttgaccgatt aaacgtaatt
     6541 cttttgcacg aattttgtca ttgatttgag tttgatcttt tgctatggtt gacacctcca
     6601 aaatttttac gaaatttgca ccaagcaaaa aggaagagca ggtataaaat acccgctctt
     6661 ccttatacac agttatgtgt aatgtgatta acctgccaac tgctttatgc gtcgctacag
     6721 gtgagaagcg ggtgcttcta cttggttcgt ttcgtattca acgttattaa tcatatcaac
     6781 aattcacatt taagtcaaca ctataactgt aattattttt attttaacct tttatttcat
     6841 ccattgacac gtcttgacgt aaatctactt gttctaatgg aatttttttc gttttatatc
     6901 gaagcttatg ataaataaag aatgctaaaa atactggaat tcccatatac gtaattaaga
     6961 agcgactaaa attaaaatct cctgttttaa taaagtcaac atcttgacca ataattacta
     7021 caatacataa aaagccagca aataatggtc cgaatgggaa taatttagca gtatatttta
     7081 atttagattt gtcataattt tgtttatcaa atgctcttct aaatcgataa tgacttactg
     7141 ctatacctac ccaagcaata aaaccagtta aaccacttgc tgcaacgata tattcgtatg
     7201 caccttttgt aaggctttgt aatacgaaaa ttatcactac aatgattgct gtaacgagta
     7261 acgacatata tggcacaccg tttttatttg ttttaccaaa tgcttcaaac gctaatttat
     7321 ctttactcat tgaatatagc attcgagttg aagcatacat acctgagtta cctgctgata
     7381 acacagacgt taaaatgact gcattcataa atgatgctgc aaacgcaaat ccagcatttt
     7441 taaacactaa tgtgaatgga gacgttgcta cattatcact accccccatt aatgcactac
     7501 tatcataagg aattaacata ccgataacaa aaatggctaa aatgtaaaat aataaaattc
     7561 tccagaatac ttgtttaatt gctttcggca cagcacgttc aggattttct gattcaccag
     7621 ccgtaatacc aattaactca gtaccttgga atgagaaacc agcgattaag aatacaccta
     7681 gaattgataa caaacttcct cctaagttgc caccaagaat tggaccttca cctttattaa
     7741 atatttcgaa tcctacaaca tgaccaccca tgattccgac aatcgttaat aaaccaattg
     7801 caatgaaaac aataactgta accactttta tcaatgccaa ccagtattca ctttcaccat
     7861 agacgcgaac tgataacgaa ttcagactaa aaattataac taagaacaac gcactccatg
     7921 cccaagcggg tatgccttgc aatggtgtcc aatattgaat gacttgtgct gcaatcgtaa
     7981 tatctgctgc tacagtcact acccagttaa accaatagtt ccaaccaagc gcaaacccta
     8041 aagatggatc aacaaatctt gtagcatatg tactaaatga acctgatact ggcaaatacg
     8101 tagccatttc gccaagtgac gtcattaaga aaaataccat tattccgata attgcgtatc
     8161 ctattaatgc acccaaagca cctgcatcat gaattgctcc accagaagtt acaaataaac
     8221 ctgtaccaat acaaccccca atcgcaatca tagaaatatg acgatcttta agtccccttt
     8281 tgacaacatt gttactttca ttttgaactt ttgacatata agctcccttt ccatgtttta
     8341 gaggccaaga aataaataac ctttattcac tttcataccc ttttttacag aacatagtga
     8401 tattaaaagt aaaaaagtgg ctacatacct ttaagatatg caaccacttt ggcaattatc
     8461 tatataaggt agcacatcac attctgtgac agtacagttc ctattcgaaa actgtcccaa
     8521 tagatataat ttatggttta tatctatttc ggcatcttta cctttcactt gttcaactta
     8581 tgtaccataa atacttctga caagttacta attaaacatg caacctctaa ctcaatttaa
     8641 tattttaact aacttgtaat atacaggatt catcacgcat aatcaaccct gtaaaacttg
     8701 atacgcaata aaagttttaa agcattttat tgcgacaact gtctatctat gttttttcaa
     8761 acgaatttca tcaactagat tccagataaa ttcatctttt tcaactgttt cttggtcttg
     8821 cgatccatat tgacgcacat tcacttgatt attttcaact tccttatccc caactacgat
     8881 ttgataaggt attttttgca tttgagcttc tctgatttta taacccattt tttcattacg
     8941 gtcatcaata cttacacgaa cgccttgaga tttcaattca tcttgtaatt ggcgcgcata
     9001 atcataatgt aaatcaacgt taactggaat gatttgaact tgttttggcg ctaaccaagt
     9061 tgggaatgca cctttagttt cttcagttaa aaatgctaca aatcgttcca ttgttgatac
     9121 aacaccacga tgaataacaa ctggacgatg atgttcacca tcttgaccaa tataagttaa
     9181 atcaaaacgt tctggtaata agaaatcaag ttgtgctgtt gataatgtct cttctttacc
     9241 catcgctgtt ttaacttgaa catctagttt cggaccatag aatgccgctt caccaatcgc
     9301 ttcttcgtac gataagccaa gctcatccgc tgcctcttta agcatatttt cagctttatt
     9361 ccacatatca tcatcatcaa agtacttttc tttatcttca gggtctctat aacttaatct
     9421 aaagctataa tcctcgaaac caaagtcttt atacacatca ataatcatgt ttacaacgcg
     9481 tttgaattct tctttaattt gatcaggtcg aacaaagata tgtgaatcat ttaaagtcat
     9541 accacgaaca cgttgtaatc ctgatacagc accacttgct tcatatctat gcatcgttcc
     9601 tagctcagcg atacggatag gtaattcacg atatgaatgt ggtttattcg cataaatcat
     9661 catatgatgt ggacagttca ttggacgtaa taccatagat tcagtttcat ctaactgcat
     9721 tggtgggaac atatcttctt gatagtgatc ccagtgacca gatgttttgt ataaatcaac
     9781 attagcaagt actggtgtat aaacgtggtc atatcccatg ctaacttctt tatcaacaat
     9841 gtaacgttca atttcacgtc taattgttgc accgttaggt aaccataatg gcaaaccagc
     9901 accaactaat tggctatttg tgaatagttc taactcttta ccaattttac gatgatcacg
     9961 ttctttacgc tcttctaaca tttgtaaatg tgctttcaat tcttttttat caaagaaagc
    10021 agtaccgtat atacgttgta acattttgtt gttactatct ccacgccagt atgcacctgc
    10081 tgtagataat agtttaaact ctttaatttt agctgttgat ggaacgtgaa ctccacgaca
    10141 taaatcagta aaatcacctt gactatataa tgttacattt tcatcttcag gaatcgcgtc
    10201 gattaattct aatttgtatt catcattgct gaataactct ttcgcttcat ctcgtgaaac
    10261 cacttttcgt tcgattttca tattttcgtt aacgatttgt ttcattgttt tttcaatttg
    10321 ttcaaagtca tcagatgaga tgttttggtc aatgtcgaag tcatagtaga atccaccttc
    10381 tattacagga cctacaccaa atttaacatt accatataac cttttaatcg cgtgtgccat
    10441 taaatgtgca gtagaatgac gtaatacctc taacgcttct tcactacctg gtgtcacaat
    10501 ttcaattgat ccatcagttt caagcggttt agttaaatct acaagttgcc cgttaaattt
    10561 gccggcaaca gcttttttac gtaatccagg actaattgat tgtgctatat cttcagtagt
    10621 agtaccttta tcaaacgcct ttttattacc atctggaaat tgaatattaa tttgttccat
    10681 gttaaaccct cctatttgct ttataaatac ttttatctga cctcgttaaa aaattgaaca
    10741 acaatcatcg ttttaacatt aaacctatta acaataaaca gggaatttat gacaccaaca
    10801 aacatttatg tctgaaaaaa acaaaaaatc ccgcccctat acaagggacg agatcgtcgt
    10861 ggtaccaccc tagttatcta atacaattta tgtattaaat atctctgctt aagataacgg
    10921 tcttgatccg ggtattcatt acaaatacca taaatgaagt agtaatcatc taatttatta
    10981 accatattct catcaaacaa tggctttctg tgtaatattg attagatcat cttgtcttca
    11041 atcatttaaa cgatttagat ttgtattcat tatacaccaa tcattttaaa attcaattgt
    11101 ttctgaaatt ttctcctgat aaaaagtatg gtgttgacaa agatttgaca cgttcaataa
    11161 tacgtgctgc tttagtcttc tcttcaccat cacgagtcat cgctaaatga tgttccaatt
    11221 cactatagtc aaaattagaa ctaaagaatg ttggtaattc atgaaccatt cgataatgta
    11281 gcaaaggtcc aattacctca tctctcaccc atggagtcac ttcttcagcc ccaatatcat
    11341 caagcattaa aatgtttgct tctcttacgc gatgtaattt cttttcaaaa gaaccatctt
    11401 taaagccacc ttttaatgtt ctaataaatt ccggtaaata aataattgtc gaacgtacct
    11461 tcttagattt gagctgattc gcaattgcac ctagaataaa agatttacct gtcccaaatg
    11521 gaccataaag gtaaaggcct ttcacttgtt ccccattagt tattgctgta caaatatcat
    11581 ctgctgccat agctacatca agacggtctc gatgattcat ataaatatct ttcaatttgg
    11641 catttaaagt atctcgttgc atatgatgag atgtaattag ctcagcttca aagcgttctt
    11701 cgtcgtactt gattttacat gggcattgta aatagcgtat tttaattcgg ttattatcaa
    11761 catataactc aggcacatgc ccctttacga aatttggaca atcagcaaat ttatgaccgt
    11821 cataatgttt ttgttgatct ttatactctt gtaacacatt taagtcttca tcaatcatag
    11881 cattcgttaa ttcagctcga tgcgcttcca aaaattgctt aacatctggg tcattgatta
    11941 cttctttttt tatcttttct attctttttt caaagtcctg cgacgtgtta attatacttt
    12001 taaattgctt cattattgac tgtcctcctc ccatttttta gataatttat ctagaaatgc
    12061 ttgtcgatct tgctctaatt gttgatcatc tacgctatta tctttagccg aatcttcttc
    12121 actaggttta tctctatttt ctaaccattt aggtgttttt tcttttgaaa tacgattacg
    12181 ctgcccatag tatgaaccac gcttttggta atttccgcta gaaccctcat ttttaggttg
    12241 attaactttt ttagcgtaat tatatgcttc tttagctgtc ttaatacctt ttttcttcca
    12301 atttgatgct atttccaaaa tatacgcttt aggaagtttc atatcttctt ttaacatgac
    12361 aaattgcaac aaaatattaa tgacgccaaa agacattttt tcacgttcaa ttaattcttc
    12421 aaccattgtc ttttgcgata tagttggttc tgattcagac caagaagcta acatatcaat
    12481 tggactcgtt tgttcaagta actcaaacca ttcatcactt tgtggctttg gattcacttc
    12541 tgaagatttg cccgccgaag atgatgtagc aggagatttc acctgtaatt taggcatttg
    12601 attttcgtgt tccattaagt aatacgagcg tgcttgttta cgcatttctt caaaggataa
    12661 ctgttgtcca cttgtaattg aatttaaaat aacatgcttc atgccatctg ctgttaaacc
    12721 atataaagtc gcgagttgtg taattaaacg ctttgcatct ttggtaacaa tgtcttgact
    12781 aataaaatgt ttacctaaca tttgtctcaa catttcaaag tcaaaagatt catttgataa
    12841 atcgatacct tggtacggtt cattaatcgg aatatcactt gtatcgatat ctatttttgt
    12901 agacggcact ttaaaaacat cagtaaattg tcttgttacc tgtttaaatt cactcaaatc
    12961 aatttgttga tactcaaagt atttcttcaa ctcatgaaat cgacgatgct cgacttcact
    13021 atataaaaag attgacaaca ttggatcatt aaaaaataaa tgtgctgaag gcggttgaat
    13081 taattggtaa acaaattgtg tttcttgttc atcatgtttg acaaacgcct ttaacaatcc
    13141 aatcgcttca agtaagtcca tttgttgtct aaactctagt aaattaattt taagttcatt
    13201 cataaaaata taatgagaaa gaatcaatgt ttcattatga ctttctttaa cgaattgagt
    13261 cataaaatga tataaaccca ctgcttgcgt tccaattagc ggtgtataca gtcgattcaa
    13321 tacctctaaa tgattcgtat ttaaatcaaa gtgttgcata actttgaatt gatcctttgg
    13381 tcttaagccg aattcgaagg cttgtcgtcc catttaagca tcactccgtt tgttttcgct
    13441 taaaatccct tgcatcgatg ctaacaattg atcaacatct ttaaattctt tatagactga
    13501 tgcaaatcta acatatgaaa cttgatcaac atgcattaac aagttcataa cgtgttcacc
    13561 tatatctcgt gaagacactt ccgtatgacc ttcatctcgt aattgccatt caaccttgtt
    13621 agttatgtct tcaagttgtt gatatctaac tggtcgtttc tcacaagaac gcacaagtcc
    13681 attaagtatc ttttctcttg aaaactgctc tcttgtgcca tcttttttca caactataag
    13741 ctgactaact tcgatatgtt caaatgtagt gaaacgtgtt ccacaatttt cacattctct
    13801 tcgtcttcga atggcattta attcatcggc atgccttgaa tctacaactt tagattgtgt
    13861 agaattacat ttcgggcatt tcattacatc accctcttta ttttgattat gcctaattat
    13921 actataaatc tagagatgaa aaaagaatcc ctcaatttaa ttcatttaac caaataatga
    13981 aacaataaaa aacattatat cgttacttat taagtaattt gcatgacaat attattgtat
    14041 taaaaataaa aaacctaact ccgaagtcag agttaggcta taaattaatt gtattaactt
    14101 gcacttacag tttcttttga tgtcaaaagt gctccaattt gctcagcaac atctacaact
    14161 ctatttgaat aaccccattc attatcatac caagcaataa cttttacttt attccctgac
    14221 atgaccattg ttgattttgc atcaataata gctgaatttg gattagtatt aaaatcaaca
    14281 gacactagtg gttgatgttc gacttctatg ataccttcta aacctgcatt ttcaaaagct
    14341 tggtttactt cttctgcagt tacttctttt tctaaatcaa caactaaatc aacgagcgat
    14401 acattctttg ttggtacacg taatgccatg ccgtgtaatt taccttctaa ttctggtaat
    14461 acttctttta aagctttcgc cgcaccagta gaagtaggaa taatgctttc attacatgaa
    14521 cgtgcacgtc ttaaatcttt atgtggatta tcaatatttt tttggtcatt tgtaatagcg
    14581 tgaacagtag tcattaaacc attaactatt ccaaactgat tatttaaaac ttttgcaact
    14641 ggaccaatgc aattagtagt acatgaagca ttactaaaaa tgtcaaatgc ttctatatct
    14701 aattggttat catttacgcc tttaactacc atttgaacat gtccaccttt tgaaggacca
    14761 gttaacaaaa cttttttggc acctgcttta atatgtgcga tggctttatc accatgatta
    14821 aatttaccag ttgcatctat agcaatatcg atatctaatt ctttccatgg caagttttca
    14881 ggattgcgat cagcaaccaa tttaatttta tgatctccaa cttgcaatcc attttcaatc
    14941 ggttcaactt ttagattata ttttccatgt gtcgtatcgt aattgattaa atgtgcaatt
    15001 gtttcgggtg gataactagc atttatcgct actacattta aatttttatt ttgtaatgca
    15061 atacgtaata ccattcttcc aattctaccc ataccattaa ttgcaatatt cgttgacatt
    15121 aaaagcaccc cttgatcttt atgtgttata ctttatggaa ttagtatagc atattatatg
    15181 aaatttgaaa gcgttttccg ttgatttatt tatctttttt ataaaaaata gtcattactt
    15241 acatacatca acgcgtacgt aaagtaatga cttatttata gtgtaatatt aatcttcttc
    15301 tccgtaattc ggcttttcaa tataaccttc ttcttctaac aatctctcaa ggttttgttt
    15361 taattcaagt ttatccccta aattatcgat aacatgatcg gccattcggc tttttttatc
    15421 aatagaaatt tggctataga cacgtgcttt cgcatcttct aatgacaaat tattacgttg
    15481 cattaaacga tccatttgta tactttcaga agtgtataca acccacactt cgtctactgt
    15541 attttccaat tcattttcaa ataataatgg aatatccatg attacattat atccttgttt
    15601 taaatattct tgcttttctt cttccataat atctcgcacg ataggatgta tgatagcatt
    15661 taattctaag cgtttttctg gatgattaaa cactagatca cccatataac gacgattcat
    15721 ctcaccattt tcatcaattg cttcatcacc aaagacttct cgtacttgag ctaaaccttt
    15781 actccccttt ttaacagctt ccctggctgc tttatcagca tctactactt taaaaccgaa
    15841 tacggataag agttctgata ctgttgattt tcctgaggcg attccacctg ttagaccaat
    15901 aactttcggc ataatttcac tctttcttta tttttgacat actggacaat aatgactatt
    15961 tcttgtcgcg atgatttttg tttcaatttg acttccacac actttgcata ccggctgctt
    16021 atatacatta agatgcaatt gcatctcacc agtttttcca tcagcatgac gataatctga
    16081 aatacttgta ccgccatatt taataccttc ttctagtact tctctaacat aataaaaaac
    16141 catttcttgt tgttggtgtg ttaagtcttt tactttttta tctggtaaaa cacctgcacg
    16201 aaacaacgct tcacatgcgt aaatatttcc acaacctgcg attactttat gatccaaaat
    16261 cacttgtttg attggtttat tcttattaga ctgttgatga attcgattta aataatacgt
    16321 caatgcttca tttgaaaaag gttcaggcgc tatttctaaa aatgaaggat aagatgctac
    16381 agacgcaaca tttctaattt ctccaaaacg acgtatatct gaataaatta actttttgtc
    16441 atttgacaac tcaaaaataa catgccaatg cttacgataa ttaggtatca taatatcttc
    16501 aagttcatct acaatgaaaa aaccgcccgc catacctaaa tgactaatta atgtacgttg
    16561 ttctcgttta ttatctagct gaaaaacgat atatttactt cttcgttcta catttgtaat
    16621 ggtatagcct tccgataaag ttttaaaagt atctaattca attcctttta taattgtttc
    16681 cttgccttga gctttacctt cgattacttt atccgaaaat ataacgtgtt caattttttg
    16741 atttataacg tagggttcaa ttcctctttt tacatgttct acttctggta attcgggcat
    16801 accattaacc tcactttatt ttgcatcata ccaggttgca ccataacttg agtctacttt
    16861 taatggaaca tctaattgca atgcattttc cattatctct tctacaaatt cactaaatga
    16921 atctacttct gacttaggta cttcaaaaat taattcatcg tgtacttgta ataatagttt
    16981 agcttgatat gttgtctctt tcattttttg agcaaattta accattgcca gtttaatgat
    17041 atctgcagca ctgccctgta ttggcgtatt catagcagta cgttcagcaa agccgcgtaa
    17101 attaaagtta cgactcgtaa tatcaggaat atagcgtcga cgatgtagca atgtttccac
    17161 gtaacctaaa gctttggcat cttttacaat atcagacata tattgtttta cacctgggaa
    17221 actagctaaa taatcatcaa tgaatgcttt tgctttttta cgagtaatac ctaaactttg
    17281 acttaaacca taatcactta tcccataaac aattccaaag ttaactgctt ttgcttgacg
    17341 acgcattaaa ctatcgactt gatcagcttc tacaccaaat actttcatag cagttgctgt
    17401 atgaatatca tcgccgttga taaatgcttc tttcatactc tcatcttgtg taatgtgtgc
    17461 taatacacgc aattcaattt gagaataatc tgctgataat ataacgctat cttttgaagt
    17521 tggtttaaag gcttttctta ttttacgccc ttcttcaagt ctaaccggaa tattttgtaa
    17581 attaggatct acacttgata aacgtccagt ttgcgctaaa gtttgattaa aacgtgtatg
    17641 gatacgttga tcatcactaa ttaccttttg caatccttca acataagtag actgtaactt
    17701 agataattga cgatattcta aaatataatc aatgatagga tgttcacctt gcaattgctc
    17761 taagacatct acagcagtag aatatcctgt tttcgtcttt ttaataacag gtaattgtaa
    17821 tgtctcaaac aatacaacac ctaattgctt aggagaattt atattaaaat cttcaccagc
    17881 tgcatcatgg atatttcgaa tcaagacgtc taatttttct tgaatttctt tttccatttc
    17941 ttctaaatca tgaacatctg taaatatacc aatttcttcc atttcactta aaatcttagc
    18001 tagcggtagc tctaaatcag ccaagagttc tacctgattg tattcttcta attgtttatc
    18061 catatttggt ttcgcaaagt aaattgcatc agtaatagaa gcaacatatg gatttaaaac
    18121 atcatcttca ggtaccttaa atttcttacc ttttccatat atactcacat cgtctttcac
    18181 aaaactttga ccgtacaatg aaacaactga ttgaacatca ctaatcgtac gagatggatc
    18241 aattatataa ctggccaaca taatatcgaa agaaatattt tgaatatcaa tccccaatct
    18301 atgtgatgct acatatgttt ttttagcatc atatacgact tttttcgaat tcggattttc
    18361 taaccatgaa actagttcga cataattatt tatgtcatcc gcattaatta caatatgttt
    18421 ctcacctgta aataaagaga attttaaaat attatttcgc aaatagttac caccgtctaa
    18481 ttcgaaatgg atggccgctt ctttcaatga agtaaaatca atattatcaa aagacgtttc
    18541 aatttcaaat gtcttttcta ttgcatcttc aacgcttgct gattgatcaa tgtcagccaa
    18601 caattgtttg aattctaact tcttaaacaa ttcgattttt tcttgttgtt catcttgatg
    18661 agtcattaac gtatcttcaa gttttacttc aatcggacta tctacattaa tcgttgctaa
    18721 ttctttactc attaatgcat cttctttgct attttgaagt ttttctttta actttttacc
    18781 tgaaatttca tctaaatgtt catagacacc ttctactgtg tcaaattggt ttagcaattt
    18841 tattgctgtt ttctctccaa cacctgcaac acctggtata ttatcagaag tatctcccat
    18901 taatcctttc atatcaataa tttgattagg tgttaacccg ttgtattttt ccgcaataaa
    18961 gtcaggtgta taatgatcaa catcagtaac acctttttta gtgtaataaa tggttacatt
    19021 atccgttgca agttgtgtta aatctcggtc tcccgtaata ataattgtct gaaagcccgc
    19081 tttatctgct tctttactta aagttccgat aatatcatct gcctcatagt tatctaattc
    19141 ataacgttta atatgataag catctaataa ttggcgaata taaggaaatt gctcacttag
    19201 ttcaggcggc gttttctggc gtccaccttt atattcacta tatttttcat gtctgaaagt
    19261 cgttttacct gcatcaaacg ctactaaaaa atgatttggc ttttcttctt ttaaaatctt
    19321 ctctagtaac attgcaaaac catatactgc attggtatga atgcctgctt tgtttgataa
    19381 caaaggtaat gcataaaaag ctctaaaact taagctatta ccatcgatta atactaattt
    19441 attcacaatt ttaacctcca gaactaattt atatatattt tagcatattc gactgtgatg
    19501 tcgtaattaa agaaactatc tcatagagta tttatacacg acctaatttc aaataaaaca
    19561 agtgcctata tagacttagc taaaaaataa aaaagacata atcatgattg ttcttgatga
    19621 gcactccata aagtcaggtt aaaagtccaa cttttgagat gcacattatc atttcatgat
    19681 tatgtctact ttttaatatt tttatgctgc tattttaaat ttttataggt aagaataagt
    19741 tatgaatcgt ctgaaccaaa tggaatatat ttaactttgg tcattttcgg taattcttca
    19801 taattattaa accactcttg gtaattttta ttgattggtg acggatgatg catataatgt
    19861 tcaaacggca atgattccac tgtataaacc tcttgtcgtg gatgatcttt aatttgttgt
    19921 tttaataact gaactctttt ttcatgttca taatgaacat aaataaatgc actaagatat
    19981 atcacagcta aaactagtga tgcaccttta ataaaattaa cattgattga cttatatttc
    20041 cgaaattctt ttaataaaat aattaaaatg attacatgta tcgtataaac aatcaaaaaa
    20101 ttacctggtt ctattggagt aacaatgact agtgtcgacg ccgaaacaca tattgcaata
    20161 agtaaggaat ataaagtgat ttgtgtttta cgatcattaa tagacaaata aatacctaca
    20221 aatatcgaaa acgcaaagta accacataca attacgttca caaaaccaac caatccaata
    20281 tctgtatttt tatttaataa aaactgattg taaaatagta aataataaag cggtaaagtg
    20341 ataaatccta tcataatgat acgacgcttt aatattgtta aatgtacata ccgatcactt
    20401 ctaagcagta gataaataat aagcgctgca atcatactta aaattataat ctgactgaaa
    20461 atgacaccat atggaagtga cgttgatatc atttctgcaa attttgaaaa gataccttga
    20521 ttatttgaaa cttgttgata ttctgatcct tcaaataaaa ttttgcgata atttggattg
    20581 gaaaacataa tgattgtacc tatagtagca atcataaatg aaaacaataa tttataatta
    20641 agtgttcgat ttgcagtaaa ttcatacaga aaagctaaaa ttaaaatgat acagtggaat
    20701 aatgtcacat tctccataaa taattgaccg aagaagcata aaacatagaa tagtacagta
    20761 acacttaccg gctgcttttc tttatatata attgcattaa tacaataata aataataaag
    20821 agtgaaatta gtgttgatgt cgcataatta taaaatcctg caaaccagcc atatgtatct
    20881 gcataaatag cacttggtaa aattaacatt aaagaaaatg ctaacaaata atagcttgtc
    20941 cacgctttac aacgtgtaat gtgcataatc atccaaatga tgcccatact aatgaggcca
    21001 tatgaaagcc aacgtaacca gcttacatgt acagctataa tttcaaagat atttccgata
    21061 tagcgaccat taagtgatgc aaatcctact tttaaaatat cagtattata attgctaaac
    21121 cattgtaaat catcgtgcat gagtggtagt aagataccca taaaagtata aaacaataat
    21181 atcgcaatta atatcaaagt tgtcttgtgt aattgaattg ttttcacttt gctaatcctc
    21241 aaatctagtt aaattttcct caacttgtag gtcgaaaaat taattcaata tttttaatgt
    21301 atttctaatt ttcacctatg catgtttcct caatcaaat
//
LOCUS       DALAGF010000040        21119 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.040, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000040 DALAGF010000000
VERSION     DALAGF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21119
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.040"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(1..211)
                     /locus_tag="O2692_001373"
                     /pseudo
     CDS             complement(1..211)
                     /locus_tag="O2692_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
     gene            complement(456..2207)
                     /gene="srrB"
                     /locus_tag="O2692_001374"
     CDS             complement(456..2207)
                     /gene="srrB"
                     /locus_tag="O2692_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HCZ4299008.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            complement(2188..2913)
                     /gene="srrA"
                     /locus_tag="O2692_001375"
     CDS             complement(2188..2913)
                     /gene="srrA"
                     /locus_tag="O2692_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HCZ4299009.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            complement(3046..3783)
                     /locus_tag="O2692_001376"
     CDS             complement(3046..3783)
                     /locus_tag="O2692_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCZ4299010.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            complement(3776..4318)
                     /gene="scpB"
                     /locus_tag="O2692_001377"
     CDS             complement(3776..4318)
                     /gene="scpB"
                     /locus_tag="O2692_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCZ4299011.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKDQLVELIENYSS
                     HGLMIQRFGMTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            complement(4311..5042)
                     /locus_tag="O2692_001378"
     CDS             complement(4311..5042)
                     /locus_tag="O2692_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCZ4299012.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            5134..5640
                     /locus_tag="O2692_001379"
     CDS             5134..5640
                     /locus_tag="O2692_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HCZ4299013.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPVFERIIKANYPDYDYNQETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            complement(5718..6605)
                     /gene="xerD"
                     /locus_tag="O2692_001380"
     CDS             complement(5718..6605)
                     /gene="xerD"
                     /locus_tag="O2692_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCZ4299014.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(6654..7103)
                     /locus_tag="O2692_001381"
     CDS             complement(6654..7103)
                     /locus_tag="O2692_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCZ4299015.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            complement(7208..7750)
                     /locus_tag="O2692_001382"
     CDS             complement(7208..7750)
                     /locus_tag="O2692_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCZ4299016.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            7832..8740
                     /locus_tag="O2692_001383"
     CDS             7832..8740
                     /locus_tag="O2692_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCZ4299017.1"
                     /translation="MQKNILKSGISLSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIFIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            complement(8956..9204)
                     /locus_tag="O2692_001384"
     CDS             complement(8956..9204)
                     /locus_tag="O2692_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299018.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDGTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(9220..9975)
                     /locus_tag="O2692_001385"
     CDS             complement(9220..9975)
                     /locus_tag="O2692_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCZ4299019.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGEIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            10117..10932
                     /gene="proC"
                     /locus_tag="O2692_001386"
     CDS             10117..10932
                     /gene="proC"
                     /locus_tag="O2692_001386"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCZ4299020.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
     gene            complement(11037..11957)
                     /gene="rnz"
                     /locus_tag="O2692_001387"
     CDS             complement(11037..11957)
                     /gene="rnz"
                     /locus_tag="O2692_001387"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCZ4299021.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKI"
     gene            12270..13754
                     /locus_tag="O2692_001388"
     CDS             12270..13754
                     /locus_tag="O2692_001388"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCZ4299022.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
     gene            13984..14850
                     /locus_tag="O2692_001389"
     CDS             13984..14850
                     /locus_tag="O2692_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCZ4299023.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(14933..16582)
                     /locus_tag="O2692_001390"
     CDS             complement(14933..16582)
                     /locus_tag="O2692_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCZ4299024.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDFG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(16598..17617)
                     /locus_tag="O2692_001391"
     CDS             complement(16598..17617)
                     /locus_tag="O2692_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCZ4299025.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETIASQNKINYQI
                     IETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTCIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            complement(17618..17872)
                     /locus_tag="O2692_001392"
     CDS             complement(17618..17872)
                     /locus_tag="O2692_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299026.1"
                     /translation="MVNVYLIFKMAKVTILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYSGWKRFPMQKLTGGD"
     gene            complement(17811..18182)
                     /locus_tag="O2692_001393"
     CDS             complement(17811..18182)
                     /locus_tag="O2692_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4299027.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKHSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            complement(18401..19807)
                     /gene="gndA"
                     /locus_tag="O2692_001394"
     CDS             complement(18401..19807)
                     /gene="gndA"
                     /locus_tag="O2692_001394"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCZ4299028.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            complement(19875..21008)
                     /locus_tag="O2692_001395"
     CDS             complement(19875..21008)
                     /locus_tag="O2692_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCZ4299029.1"
                     /translation="MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAVVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARHSK"
BASE COUNT         6743 a         3880 c         3039 g         7457 t
ORIGIN      
        1 ttttcaaatt ctttacgatg cttcttcgca aattcaatcg cattttttct gcaggcggtt
       61 gaaagccttg gcctacatat tttgaagctt ccatttcttc tggtacagat tttgtttctg
      121 tttttgtgtc tttttttgat tcattttcca tcgtggaaca tccccctaaa attaatgtcg
      181 tagctaaaac tgatccaatg aattttttca tatgacacct cgaataattt aattaataca
      241 atatatctat attttaccct atttttataa tcaaataaat aactttatca aactttatat
      301 ttttgtataa attagtaata tatttaatag ttgatattcg caaaaaccct aagttctaat
      361 ccacatacaa aattccccac atcaatcaaa aaacgcatgc tataaattag agtctctaat
      421 caataacatg cgttctgaaa taactatatt caattttatt ctggttttgg tagtttaata
      481 ataaatgttg tgcctttccc taattcgctt ttaacatcta tggaaccacc atgctcttcg
      541 ataatcattt tacaaatgaa caaacctaaa ccggtacctt gtttacctcg cgttctcgct
      601 gcatcaactt tataaaaacg atcaaatact tgttgtaaat gttctggtgc aatgcctgta
      661 cctgtatctt taatgtataa aatatcttcg ctttcatttt catcacaagt aattgcaatt
      721 tcatctccag gtttcgtata acgtgatgca ttatcaatta agttcgttag tacttggtcc
      781 atgcgatcca tatcataact ccaaacacgc ttcttacaat aattaaaagt catatttaga
      841 cctaaatcat cagcttgttg gcgatactta attttcatct tatctagtaa cgctgcaata
      901 ggctgaactt ctttatttac ggataaccct tcagcatcca tgcgtgcgac atttaacaat
      961 tcattaacta aacgatttaa acgtttcgat tcatcaagga caatggcaag cgattctttt
     1021 atttcatccg gttctgtaac aataccatct acaattgatt cagtataacc ttgaagtaat
     1081 gatatcggtg tacgtaattc atgtgataca ttagcaatga aatctttctt catttgatct
     1141 agattgtgct cattagtcat atcacgaact gtcacaacaa caccactttt acctccctgt
     1201 tcaatcttgt cgatatagct tgtggtcaca acaaagaatc gtgcattcat ttctaaatcg
     1261 cgcatttcag tttgttttga tttaaaagta tcttctattt gtcttaataa gaaagcttta
     1321 gcatcttcat caatattgtc cataatatca ttcgccatct tattagataa gataatttgt
     1381 cgactctcat taatacctag gacaccttct accatagagt taattaagct gtctctaata
     1441 tttttagatg tggataatgc gtcgacatgc tcttcgattt ctgtactcat ctgattaaat
     1501 gcctgcgata attgaccaat ttcatctttc gttgtgacag aaggtttata agagtaatcc
     1561 ccttcagata cacgtgtagc ttggtctctt aaacgtctta aaggttttgt aattcttgac
     1621 gataagaaaa acgcaaagac tgttgtaatt gttaagaaaa taacagccgt aattatggtg
     1681 ataatcgtaa tagcattatt tgtatcttcg attgatttca agtctttata tataaagact
     1741 ccactatatt tgctatgact attcttctgt gcttttgttg gatagcctaa caaaatatat
     1801 gtttgagatg agcccttttc tttaatcgtt acatttcgag taacagattt acctttatca
     1861 aacacatcgt caaaatggtc gttgttgact acttcattca acatttgctt tttaatatta
     1921 gaaagtgaag ccgttgattg acgatgttta ttatttataa tcatcaaccc accaggattt
     1981 tcaattaatg tttgactata ttttattgct tcttctttat tatgtgattg ttcgaccagt
     2041 gaacttatac gtctagcatc ttctcttatg gcattttcgg tttcttgtgt gaaatagtat
     2101 tgcataaagg taattaaagc aatacttaat aaaattaaaa ctgtcgtcac tattaaaata
     2161 atagttaacc acagtttaat tacgacacta tttagccggc tcatcattag atttaacctc
     2221 aaatttatac ccaacgcccc agactgtttg aatcatatgc gcagcttcac tagacacacg
     2281 atttaacttt tctctaagtc gtttaacatg agtatcaact gttcttaaat caccatagaa
     2341 ttcataatgc caaacttctt ttaataattg ttcacggtca aatactttat ttggtgtttt
     2401 agctaaatat attaataatt cgtactcttt aggagtcaaa ttaacttctt gattatcagc
     2461 aagtacgcga tgtgcatcat tatctatttc taaatgttta aattcaatca catcacgtgc
     2521 gtgaggttcg ctttgttcta cagttgtaga ttgcgttctt cttagaagtg ctttaactct
     2581 taagactact tctcttggtg aaaatggttt gacgatataa tcatctgcac cagattcaaa
     2641 accttcaaca cggtttgttt cttcaccttt agcagtcaac ataataatcg gtgtttgttt
     2701 atgttcacgc aatttagttg ccacctggat accatccatt tcaggcaaca ttaaatctag
     2761 tagtatgcaa gcataattat tctccattgc aagttcataa gcctcttggc cattacttgc
     2821 ttcatggatt tcaaaagatt ctctttctaa atacatttta agtaatcttc tgattctatc
     2881 ctcatcatct acgataagta tttcgttcga catacaggtc atacctccca cacatgcttt
     2941 tctttacaaa agtattatat cacaggaaag cgcatacagt tattaaaaac ctaaaatttt
     3001 gtgaaaaaat tctcaaactt tgtgataaat acatatttta tttaattatt tcccatgttc
     3061 tgctaaatga cgcatcactt ttacttcatg aggcgtcaat acacgtcctt caccagcatt
     3121 caaaccgaca acatttaaag gcccatattc aatacgagac agtttcgtca cttgatgacc
     3181 aaaatgttcg aacattcttc tgacttggcg attacgacct tctgtaattg taatttcaac
     3241 caatgttgtg tttttatctt tatcttgttt cttaactttc acttcagccg gttgcgtcat
     3301 accatcttct aattcaatac ctttttctag cgctttcact tcttctctca ttaaataacc
     3361 ttttaatttc gcaacatatt ttttcttaat ttgatatctt ggatgtgtca ttaaattagt
     3421 aaattcacca tcatttgtga gtaataacaa tccagaagta tcatagtcta aacgaccaac
     3481 aggataaata cgtgcttcta tatctttaaa gtaatctgta accactgtac gtcctctatc
     3541 atcagataca cttgttatca cttgagttgg tttatggaat aaaatgtaaa ttttgtcttc
     3601 tagttctatt ttaatacctt caacttcaat cgtatctgat ggcttcactt ttgttcctaa
     3661 ttcagtgaca gtcgtaccat tcactttcac ttttccttca gaaattaaag tttctgcctt
     3721 acgtcttgaa gtataaccgc tattagctat acgtttttgt aatcgttcta attctttagt
     3781 cattattttc tcctttttga ttgactagat tactgaaaaa agcatccatt tcttcatcgt
     3841 cttcttcagt tgtcggcaaa tcttctatat tggaaatacc aaatacattt aaaaataaat
     3901 cagtagtaat taactgttgg ctacgctgtt cattaaccac tttagcctca actagtcctt
     3961 tggcaatcaa tgtcttaact gcaccatctg aattgatact acgaattaat tcaatatcac
     4021 ttcttgataa tggctggtta taagcaataa ttgatagtac ttccattgct gcttgtgata
     4081 atttcatttg tgacttttgt tcaattaatt gttcaatata cgttgccgct tcttttttag
     4141 tcgttaaaac atacgtcatt ccaaatcgtt gtatcattaa tccatgtgat gaataatttt
     4201 caattaattc aacgagttgg tctttcgaca tatctaatat ttctaatagt tgtttttcat
     4261 ctaaaccttc atcgccagct gtaaataaaa gcgactctaa tataccatga ttatccaaaa
     4321 tggtagttca ctcctctaat aatgttaata tcttcaaaat tacgttgctg ctcaatatta
     4381 attattcctg cttttgacat ctctaaaata gctaaaaagt gagtgactac ttgttcaatt
     4441 ggctcagaaa acgtaaacag actaaagaag ttaaaatgat ctttatcttt caatctcgat
     4501 gtcacttgtt ctgtagcttg ttgaatggta aatgtctctt ttcggatttc aacagattta
     4561 ggtgtattta actcaactct atttttaact ctttgataag ctacaattaa ttcagttaaa
     4621 tcaatcgtat gatttggatc ccaagattca tctgtttcca aatgagataa atctgtcggt
     4681 ctttttgtaa aataaaaatc tctttcttct ttcatgtcat ttaaaatagc agtatattct
     4741 ttataatttt gatattctat taaacgccca actaaatctt cccgtgggtc atcatcaaca
     4801 tccatatctg atgttgattg tggtaatagc atcttacttt taatcattaa gagttctgac
     4861 gctaatacta ggtattcact tgcaatatta atttcaagct gtttcattgc atgaacgtac
     4921 tgcatatact gctctgttaa tgcttgcata ggaatatcat aaatatctat ttcaaatttt
     4981 tggataagat gcagcaataa atctaatggt ccattgaaag catctaattt aacttcatac
     5041 ataatatcta cctcgtattg cgtcatatgt gcataattct ctaaaatatt atagcatgaa
     5101 aataacttac ttttaaattg gggtgataac aaaatgcaac aggcactgat aaatttctat
     5161 tatcaatttc atactaagca acattatttc ctatgtcatg acattttaga agatgcatgg
     5221 aaagctgaaa ataactacag taagcaagat gcagttgtta gtttaatctt gtttgcaact
     5281 gcttgttacc attatcgccg caataattta aaaggcgcct ataagtcttt taataaatct
     5341 aaagaaataa tacaaaatgc taaagataga gatgcgttat accttaattt aaatgattat
     5401 cagttgttaa ttgagcaaca gattgcaaaa ttgaatgcag caaagccttt ttcatctgtt
     5461 atattaccaa taactccagt ctttgaacgc atcatcaagg ccaactatcc agactatgat
     5521 tataatcagg aaacagcaac agatcctttt attgtagacc atcacatgcg ccgtgatcgt
     5581 tcagaagtga ttgcagcaaa agaagaagcg attcaattaa gaaaacatag acgtaattaa
     5641 tcaaccaatt taaaaaagcc acaatagcac agacattttt agtcatgtgt attgtgactt
     5701 ttgagttatt gttcacttta tgctctagga tgaaattggt tatacatttt tctaatttga
     5761 gatttcgaaa catgtgtata gagttgggta gtagatatgt cagagtgacc taacatctct
     5821 tgcactgctc ttaaatctgc gccattttcc aataaatgtg tcgcaaaaga gtggcgtaac
     5881 gtatgtggcg ttaacgtctt tttaatgttt gcctttacac cattttgttt aatcattttc
     5941 catattgctt gtcgtgataa aggtttacca tgcatattta aaaataagac ttcagtaaca
     6001 gtctttttta aaagttgcgg tctaatcgtt tcaatataag tagttaagta ctcaatgact
     6061 gcgtcgccta atggtacaat tctttcttta tcgcctttac caaatacgcg tacaaatccc
     6121 attattaagt tcacgttttc taactctaaa tgtatcaatt cagatacacg cattcccgtt
     6181 gcgtacagaa gttctaacat cgtacgatca cgatatccat taattttatt taaatctggc
     6241 gtttctaata aagccaacac ttcgtcaaca tttaaaacgt caggcaattt tttgtcatat
     6301 tttggtgaat ctaataatac cgttggatct ttcgccgcat atttttctct tatagcaaat
     6361 tgatgaaaac tacggattgt tgaaataaat cgcgcaatag atttagcaga ttgcccttgg
     6421 tctattaaat gccccaaaca ctcttgaatt aattgtcgat ctataaaatc aatatgcgag
     6481 atatgatgtt cagtcatata atcttgatac tttttcaaat cacgtctata agcaccaatt
     6541 gtgttagaac ttagtccttt ttcaatttgt ataaaacgca aatattcttc aataattgtt
     6601 tccataacca cacctctaaa aaaagcgaag gttaatttaa cctacgcaat ttactatcct
     6661 ttacctttag cttggcacgt ttcacacaca ccatggaaag ttaaacgatg atctaaaatt
     6721 ttaaaattga actcattttc aactcgattt tcaacttctg gtaacaaatc ttcatcgatt
     6781 tcatctacac gaccacattc catacatact aaatgatggt ggaaatgttt tgcgccttct
     6841 tttcttaaat caaaacgagc gacgccatca ccaaagttaa ttttgtcgac aacttttagt
     6901 tcagctaaca actctaacgt tctgtatact gtcgccaagc caatttcagg cgctttatct
     6961 tttactttca gatatacgtc ttcagcactt agatgatctt tttcattttc aattagaact
     7021 ctaacagtag cttcgcgttg tggcgttagc ttatatgatg attgttgtaa ttgttgctta
     7081 acgcgattta atcgttcttc caacgatgtc cactccccta ctaataatta aaatcattac
     7141 aaattatttc aaacttcaca attaaaacta acagttttct caataaaatg caagcttttc
     7201 tcatttgtta tttagaatga ttataattta ataataggtg ttgcaatgca atgatagtct
     7261 tcgcatcttc tatttcttta ttcattagca tcgatttaac attctcaatc ggaactttaa
     7321 tgacttcgac aaattcatct tcatctaagt gaactgtacc ttcttctaaa ttatccgtaa
     7381 aatatattga taattgttca tcgcaaaaac ctggtgaacc atacatatct acaacatgtg
     7441 ttaactcttt ggcaatatat cctgtttctt cttctaattc acgttttgcc gcttcgactc
     7501 tatcttcatc atcttctaat ttaccagctg gaatttctag caatggtttt tctactggtt
     7561 tacgatactg tttcactaat acgacttctt ttttaggtgt cactgcacaa acagcaactg
     7621 caccattatg ataaactaat tctcttgttg acgtttcacc gtttggtaat gtcactgtat
     7681 gaatttctac atctacaatt ttgccattat aaataactgt tcgatcaatt gttttttcat
     7741 ttaaatccat tataatcacg ttcctttaaa ttcatattat atattgatac actatgcttg
     7801 ttagctaaag tgtatcgaaa ggagaacaga catgcaaaaa aatatattaa aaagtggtat
     7861 ttcattatct gaattaggtc taggttgcat gagtttaggt acagatttaa aaaaagccga
     7921 acaaattata gattgtgctg ttgaaaatgg tatcacttat tttgatacag cagatatgta
     7981 cgataaaggt ataaatgaat cagttgttgg taaggcactt cttaaatatc aacaacgcga
     8041 tgatattttt attggtacaa aagtaggcaa tcgtttaaca aaagatggca gtacaacatg
     8101 ggatccgagt aaatcctata ttaaagaggc agttaaaggt tcactaaagc gtttaggtat
     8161 cgatcatatc gatttatatc aacttcatgg cggaaccatt gatgacccat tagacgaaac
     8221 aataagcgca tttgatgaat tgaaacaaga aggaattata cgtgcttacg gtatttcttc
     8281 tattcgccca aatgtaattg attattattt aaaacatagt caaatcgaaa cgataatgtc
     8341 tcaattcaat ttgattgata atcgtccaga atcattatta gatgcaattc acaacaatga
     8401 tgttaaagta ttggcaagag gacctgtgtc taaaggatta ttaacttcaa acagtgttaa
     8461 tgtgctcgac aataaattta aagatggtat ttttgattat tctcatgatg aattgggtga
     8521 aacaatagcc tctattaaag aaattgaaag taatttatct gcattgacat ttagttattt
     8581 aacatcacat gacgtgcttg gttccatcat tgtaggtgca agtagcgtcg accaattaaa
     8641 agaaaatatt gaaaactatc atactaaagt tagtttagat cagattaaaa cagcaagagc
     8701 tcgtgtaaag gatttggaat ataccaatca tttagtgtag aagtcatttt cagtaataaa
     8761 aacagcagca tgaggcgttt cattataaaa atgccttact gctgttgttt atgtacaatt
     8821 cgctataatt tatgattatg attactcact tatgatagaa attaaagcgt tgtcctcacg
     8881 catcagtatt tagtaatttc gccttgcggc attgccttaa gcaaacttct gccacttcat
     8941 ctcttaataa ttttattaaa acatctttct atatttcact tcgcatgttg attcatcatt
     9001 attagttatt atttgtacac ccagcacatt tccttgcaac acaagtagtt tgaatttttc
     9061 acaagtataa tataatgtac cgtctgaaat ttggtctaca gaaatatcgc ctaaaatatc
     9121 cagcactgta aattcttcaa atactgatag ttgttccgca tatcgtacac aaagtcttac
     9181 cacactctcc gattgacagt tcattgccat cccacctatt tatgctttat ttttaaataa
     9241 tttagggaaa catcgttcaa aaaatctagg cgcaatttga tacattttca acgcatgatg
     9301 catccattta ggccgattaa tttccaattg ttttgtttta atgccataaa tgatatcttc
     9361 tgcaagctga ttagcatcaa gcataatttc ccccatcttt ttagcatact tcattgatgg
     9421 gtcggctttt tgatgaaaag gtgtatcaat cgggccaaca ttaactgtca tgatatgtaa
     9481 gtttggtgac tctagtctta aagcattcat taatgcataa aaccctgctt tcgatgcccc
     9541 ataatgtgca gcatttgctt gtgtggaaaa tgcagcttga cttgaaatac ctacaatatg
     9601 tgcgttagat gttaaatatg gtctcaacac agtatataaa acattaaaac taattaaatt
     9661 aagctgatac gtttcaatca tttctgaaaa actatggtct gaaatagatt tgaaataacc
     9721 taaacctgca ctataaatga atccatcgaa tgatgtattg tcttcaaatt gcagtgcctg
     9781 tatcgacttc aaatcattta agtcacaagg aataacattt atagttttcc ccaattcctg
     9841 ttcaaagatt ctagttgctt tatcaacatc acgcaccaac aacgttacat gcactttatt
     9901 ttctagtaac tttcggacaa tcgataaacc taaaccactc gtaccaccag tcactataaa
     9961 atgttgtcct ttcatcaatt aaccttcctt ttcaattata tagaatgcaa tttatcaact
    10021 ttacataatt gagacaagtt gattatcttt cctaatatat atacaataat aagaaaatat
    10081 aacatacaaa tcaaaaacta aagggatgtg acgttaatga aactcgtatt ttatggagct
    10141 ggtaatatgg cacaagctat atttacagga attattaact caagcaactt agatgccaat
    10201 gatatatatt taacaaataa atctaatgaa caagctttaa aagcattcgc tgaaaaacta
    10261 ggtgttaact atagttatga tgatgcgaca ttattaaaag atgcagatta tgtattttta
    10321 ggtaccaaac cacatgattt tgatgctcta gcaacacgca tcaaaccaca tattacaaaa
    10381 gacaattgct tcatttcaat tatggcaggt attccgattg attatattaa acaacaatta
    10441 gaatgccaaa atccagttgc tagaattatg ccaaacacaa atgcgcaagt tggacactct
    10501 gttactggca ttagtttttc aaacaacttt gaccctaaat ctaaagatga aattaacgat
    10561 ttagttaaag catttggttc tgtaattgaa gtatcagaag atcatttaca tcaagtaaca
    10621 gctatcaccg gaagcggccc agcattttta tatcatgtat tcgagcaata tgttaaagct
    10681 ggtacgaaac ttggtctaga aaaagaacaa gttgaagaat ctatacgcaa ccttattata
    10741 ggtacaagta agatgattga acgttcagat ttgagcatgg ctcaattaag aaaaaatatt
    10801 acctctaaag gtggtacgac acaagctggc cttgatacat tgtcacaata tgatttagta
    10861 tctattttcg aagattgtct aaacgctgcc gtcgaccgta gtattgaact ttctaatata
    10921 gaagaccaat aaaaacaaac ccgccaacac atgtatgcat catcgcaagc actgtgtttg
    10981 acgggttatt tttataattt attgttattt ggcaagcatt gtttattact ttgtcattag
    11041 attttaaaac tatcaaaatc ttttacaaaa ttaaaattag gtgtatcttc attttgtatc
    11101 aatgtttgat aaatttcatt tatatcttct gtattatagc gattgctcaa atgtgtaatc
    11161 aacgtacgtt taacattggc ttcttttatc aatgcaaata cgtcttcaat atggctatga
    11221 tgataattgt tggctaaatg cttttcacca tctatatagg tcgcttcatg taccatcaca
    11281 tcagcatctc tagaaatcac acgttcatta gaacatggtt ttgtatcacc aaaaattgct
    11341 acaactggac cctgtttgga ctcacctcta aaatcttttg attgataaac ttgaccatta
    11401 tgttcaaatg tatcatgaga ttttacttct tgatatttag gacctggttc aagaccaatg
    11461 ttttttaacg cttcaacatt gattgtacct gtagtttcag gtgccattac tctatatcca
    11521 tatgatggaa taccatgatt aagtaaatgc gcctctacag taaaaccatc atgatgatat
    11581 gtcagatgat catcgatttc aatatatgta attggatagt ttaaatgtga ctctgataaa
    11641 ttcatagaca tttccacata tgctttaatt ccttttggtc caaccaatgt aagcggcttc
    11701 tgttcaccgc cctgaaaaga acgactagaa agtaatcctg gcaaaccaaa aatatgatcg
    11761 ccatgcatat gagtaataaa tatatgtgtc acttttccta atttaattgc atgatgtaaa
    11821 atttggtgct gtgtaccttc accaacgtcg aaaagccata tggaattgga atatggttct
    11881 aaatttaagg cgattgcttg tgtatttctc tcttttgtag gcaaacctgc actcgttcca
    11941 aaaaatgtaa cttccatata tgcccctcct tttcttcaat tcattttatc ataaaatttg
    12001 tatcaattta gttttaattt atacttgaat caaaatctgt taaaaattcg tctttgctag
    12061 ttaatttttc taactttaac gtagacataa ctatataaat tttgataatt acgttatact
    12121 tatcattaat aagtatcaca ttaaacatga tacatgaatc gatatttcat ttaagacact
    12181 gcatacagtc gagcatattg tatgacctac tgaatggatt atcttataat aataaatcat
    12241 atatctaatt aagaattgag gttttaatct tgagtactaa aaacaaacac atcccatgtt
    12301 taatcacaat ctttggtgca actggtgact taagccatcg taagttgttt ccatcaatat
    12361 tccatctcta ccaacaagac aatttagatg aacatattgc catcatcggt attggacgtc
    12421 gtgacattac taatgatgat ttccgtaatc aagtaaaatc atcaattcaa aagcacgtaa
    12481 aagatacaaa caaaattgac gcgtttatgg aacatgtctt ctatcataga catgatgtta
    12541 gtaatgaaga aagctatcaa gaattactag attttagtaa tgaattagat agccaatttg
    12601 aattaaaagg taatcgacta ttctatttag caatggcacc acaattcttt ggcgttattt
    12661 ctgattatct aaaatcttct ggtcttactg atacaaaagg atttaaacgc cttgttatcg
    12721 aaaaaccatt cggtagtgat ttaaaatcag ccgaagcatt aaacaatcaa attcgtaaat
    12781 catttaaaga agaagaaatt tatcgtattg accactattt aggaaaagac atggttcaaa
    12841 atatcgaggt attacgtttt gcgaatgcga tgtttgaacc attatggaat aacaaatata
    12901 tttcaaacat ccaagttaca tcttctgaaa tactaggtgt tgaagatcgt ggtggttatt
    12961 atgaatcaag tggcgcgcta aaagatatgg tgcaaaacca catgttacaa atggttgcat
    13021 tattagctat ggaagcacct attagtttaa atagtgaaga tatccgtgct gagaaagtaa
    13081 aagtacttaa atcactgcgt catttccaat ctgaagatgt taaaaagaac tttgttcgtg
    13141 gtcaatatgg cgaaggctat atcgatggta aacaagttaa agcataccgt gatgaagatc
    13201 gcgttgcaga tgactctaac acacctacct ttgtttcagg taaattaaca attgataact
    13261 ttagatgggc tggtgtacca ttctatattc gtactggtaa acgtatgaaa tctaaaacaa
    13321 ttcaagttgt cgttgaattt aaagaagtac caatgaactt atactatgaa actgataaac
    13381 tgttagattc aaacctatta gtaatcaata tccaacctaa tgaaggtgta tctttacatc
    13441 taaatgctaa gaaaaataca caaggtatcg aaacagaacc tgtccaattg tcttactcaa
    13501 tgagcgctca agataaaatg aatactgtag atgcatatga aaatctatta tttgattgtc
    13561 ttaaaggtga tgccactaac ttcacgcact gggaagaatt aaaatcaaca tggaaatttg
    13621 ttgatgcaat tcaagatgaa tggaatatgg ttgatccaga attccctaac tatgaatcag
    13681 gtactaatgg tccattagaa agtgatttac tacttgctcg tgatggtaac cattggtggg
    13741 acgatattca ataattgaat taaaacgcac atgttaaaca aaaataaatg agcgaatgac
    13801 aaggattgat tacgaatcaa gtaaatcaat tgttctatca ttcgcttttt ttctagacta
    13861 tattatgaaa ttatatttta caatgcccaa aactatttta ataatcattg aacaaatggg
    13921 tgtataattt atagaaataa tgtagaataa aaataaatga ttgaattaat tggagtgaaa
    13981 gttttggacg ttatcaagca aatacaacag gcaattgttt atattgaaga tcgtttatta
    14041 gagcctttca atttgcaaga attaagtgat tacgttggtc tttcgccata ccatcttgat
    14101 caatcattta aaatgattgt cggcttatct ccagaagctt atgcacgcgc gcgtaaaatg
    14161 acactcgctg caaatgatgt gattaatggt gctacacgac ttgtagatat cgctaaaaaa
    14221 tatcactatg caaattcaaa tgattttgca aatgatttta gtgattttca cggcgtatca
    14281 cctattcaag cctctactaa aaaagatgaa ttacaaattc aagagcgatt atatatcaaa
    14341 ttatcaacta ctgagagagc accttatcca tacagattag aagagacaga tgatatttca
    14401 ttggttggat atgcacgatt tatagacact aagtatttgt cacatccttt taatgttccg
    14461 gattttttag aagacttgct cattgatggt aaaattaaag agttacgacg atataatgac
    14521 gttagtccat ttgaactatt tgttattagt tgtcctcttg aaaatggttt agaaatattt
    14581 gtaggtgtac caagtgaacg ttatcctgca cacttagaaa gtcgattttt acctggcaaa
    14641 cattgtgcga aattcaattt acaaggtgaa attgattatg caactaatga agcttggtac
    14701 tatattgaat caagtttgca gttaacattg ccatatgaac gaaatgattt atatgttgaa
    14761 gtgtaccctc tcgatatttc atttaatgac ccattcacta aaattcagct ttggattcct
    14821 gttaaacaga gtccttatga cgaagattaa ataataaaaa acaaagaagc cccctaatat
    14881 atctataggt ctacaaatgg ccttagattc tattaggggg catattaata tgttaattta
    14941 gttcgataac acatgcttca tatggacgta actgttttaa attaactttg gcatcataat
    15001 taaatagctt tacttctcca tggcttaaat caaatggtac agttaattct gcttcgtggt
    15061 tagtaagatt acctacaata agaacttgct tttcatttaa tgttctcgtg tacgcaaaaa
    15121 cttgtgaatt ttcagcatct actaaatcaa attgaccata tacgtataca tcattagact
    15181 ttcttaattg aattaaatct ttataaaatt gtaatactga atgctcatct tctaattgtt
    15241 gtgcaacatt gatagtttta taattcggat tcactgggaa ccacggttca ccatttgtaa
    15301 atcctccatt taacgtatca tcccattgca ttggtgtgcg agaattatct cggttctcat
    15361 ctttatattt cgcaagtaaa gcgtctacat ctccaccttg agctttcact atttgatagt
    15421 catttttaac agcaacatcg ttaaacgttt caatactttc aaatggataa ttcgtcatac
    15481 caatttcttg accttgataa atgaatggcg taccttgttg caagaaataa acagctgcat
    15541 gacttgttgc tgattcatac caatacttgt catcgtcacc ccacgtcgat acacgtcgtg
    15601 gttggtcgtg attttcaata aacaacgcat tccaaccttt attttcaagt tgtttttgcc
    15661 atctatttaa tacagattta tacgaattta catcaaagtg agaatcacca ctattccaca
    15721 gtcccaaatg ttcaaattgg aatatcatat taaatttacc attttcttcc ccgacccagt
    15781 catcagcatc atcagggctt acaccattcg cttcaccaac agtcataatg tcatacttac
    15841 ttaatgagcg atctttcatc tcttgtaacc aagtttgtat acctggctga ttcatatcta
    15901 catcaaatgc tggggcatat gttttaccct caggtacagg taagtcaccc gcttcaaacg
    15961 tcttcttaat atgcgtaatt gcatctactc taaatccatc aatgccttta tcaaaccacc
    16021 agttcatcat ttcaaataca gcatctctaa cttccggatt accccaattc aaatcaggtt
    16081 gttttttact gaataaatgg aaataatatt gctcagtatt agcatcatat tcccatgtag
    16141 atccattaaa tatactttcc cagttgttag gttcagagcc atctggcttt ggatcttgcc
    16201 aaatgtacca atcacgtttg ggattgtctt tactagattt ggattctata aaccaaggat
    16261 gttcatcaga tgtatgattt acaactaaat ctaaaataag cttcatgcct ctatcatgaa
    16321 caccttttaa taaacgatca aagtcttcca tcgttccaaa ttcatccata atctcttggt
    16381 agtcactaat atcataacca ttgtcatcat taggtgattt aaacattgga ctgagccaaa
    16441 tgacatcgat accgaaatct tttaagtagt ccaatttatc aatcattcca ggtaaatccc
    16501 caataccatc gtgattacta tcattaaaac ttcttggata tacttgatat gctactgctt
    16561 ctttccacca ttgcttattc attttaaaac tcctttgcta tcgctgtgtt gattttctta
    16621 tttttaattc tgtatctata atgacgagtt caataacatc ctgtgctttg tttttcaata
    16681 tatttaaaat tgctgcacca gcctgttgac ctaacattcg aggcttgatg tcaatacagg
    16741 tttgtggtgg tgacgcaatt tcggttaaat aagaatcatt gaacgttgct gtcattacat
    16801 ctttcggaat ttcaatatta agttcatata ggacacttaa aatcgctaaa tgtaacatag
    16861 catctaacga aatgattgcc tgtttaatat ttgggtcctt caaacgcgta tgtagatttt
    16921 gcatgtaatt aaaaataact tctctttcat tactagtctc aataatttga taattaattt
    16981 tattttgaga agctatcgtt tcaaatcctt gaattctatc ttttgaaact tcaaaatttc
    17041 ctttttctgt aataaatatt aattcatcta caccttgttc aataacatgt cgtgtcaaat
    17101 tttcagaagc taatatatta tcattatcta tatgtgtaaa ttgatgatct atatccgatg
    17161 taggcttacc aatcacaata aatggcatgc tttcatcaat taacatttgt ttaatcggat
    17221 cattttcttt tgaatagagc agtataaacg catcaaccat tcgttgttta atcattttat
    17281 aaacttcatc cattaaatca ttcatattat ttgagactgt cgtttgtgta ccatagccat
    17341 gctggttaca cgtttcagaa attcctagca atacattgat gtagaatgga ttcagtcgaa
    17401 taggctcctc agaccctttt aacactaaac caattttata tgtttggttt gtaattaaag
    17461 ttctagcagc ggtattagga aaataattca attcttccat aactttcttc acttttgaaa
    17521 ttgtcgcttc gctaatacgt tgatttcctt ttataactct tgaaactgtc gaaggagaaa
    17581 caccggcttt tagtgcaaca tctttaatcg taaccattta atcacctcct gttaatttct
    17641 gcattggaaa acgcttccaa ccactgtata ataccagttt agtcacactt tctaaaaaag
    17701 tcaaaagatt tgtgcaaacg attgcataaa acgataaaaa taaaaccttc atactgaaat
    17761 tcaatccgaa aatcaatata aaggtttgta taaatattaa aatcgattgt ttagtcacta
    17821 actgcaaaat agttaccttg gccatcttga aaattaaata cacgttgacc attcatttct
    17881 actatatcat gcccagttaa acctaaatca tttaattttg agtataatgc atcaaagttt
    17941 ttctctttaa acattaaaga tggtgttcct aggttcactt ccgggctatg cttttcaata
    18001 aattcttttg ccataatcgt caatgacgtt tcagcatctt tggtaggtga tacttcaact
    18061 gcaacatagt cctcagctaa cggtgtttca cttacaacaa caaattctaa agtttctgtc
    18121 caaaatgctt tcgctttttc gacatcatca acatataaca taacttgatt taacttttcc
    18181 ataaaatagt acctctattt ctctatagta catgctatca taacacagta aatattttat
    18241 tacttcacaa aatgcttaaa aatatggcgg gatgctttta aggtcaagga taatacttgt
    18301 gtaatttttt ataggttgta gctactctat cacactctct tttatattta tcaaaagata
    18361 taaaaaagga tagtatcttt caactatcct ttaatcaata ttattcttca atccattgtg
    18421 tatggaatac gccttcttta tcttttcttt cgtacgtatg agcaccgaag tagtcacgtt
    18481 gtgcttgaat taagtttgca ggtaaatcag cagcacggta actatcatag taattaatac
    18541 ttgatgagaa accaggtgtt ggtacaccat tttgaacacc agttgcgaca acatcacgta
    18601 acgcatcttg atattcagta acgatgtttt taaagtaagg atctagcaat aagttttgta
    18661 atcctggatt attatcgtaa gcatctttga tcttttgtaa gaattgtgca cggataatgc
    18721 aaccttctct ccaaatcata gctaaatcac caagttttaa attccattca ttatcttcac
    18781 ttgctttacg catttgcgcg aaaccttgtg cataagaaca aattttactc atatataatg
    18841 ctttacgaat tttttctaaa aagtctttct tgtcaccatc aaatgatgct tttggaccat
    18901 ttaattcttt agaagcattt acgcgctctt ctttgattga agagataaaa cgtgcaaata
    18961 cagattcagt aatgattgtt aatggaatac ctaattctaa tgcgttaatt gaagtccatt
    19021 ttcctgtacc tttttgacct gcagtatcaa gaattttttc aactaatgct tctttatttt
    19081 catctaattt catgaaaata tcaccagtga tttcaattaa ataactttct aattcaccag
    19141 cattccagtc tttgaacgtt tgagcaatgt cttcatgaga catgcctaat aattctttca
    19201 tcatagcata actttctgca attaattgca tgtcagcata ttcgatacca ttgtgtacca
    19261 ttttcacata gtgtccagca ccattaggtc caatataagt aacacatgaa gcaccgtctt
    19321 ttgcctttgc agcaattgca tcaagaatat ctgcaacttt gttataagct tcttcttgtc
    19381 cacccggcat taatgacgga ccagttaacg ctccaatttc accaccagaa acgcccatac
    19441 caataaagtt gattgcactt tgtgctaatg ctttattacg tctgatagta tcttgatagt
    19501 ttgtattacc accatcaatt aaaatatctc catcatctaa taaaggtaac aaactatcaa
    19561 tcgttgcgtc cgtagcttta cctgcttgaa ccattaataa aattttacgt ggtttttcta
    19621 aagaattaac aaattcttcc aatgaatacg ttggatgaat atttttccct tttgattctt
    19681 caaccattaa atcagttttt tcacttgagc ggttaaatac agatacacta tatccgcgtg
    19741 attcaatatt ccaagctagg tttttaccca taacggctaa accaataact ccaatttgtt
    19801 gtgtcatatt acttacctca cttgttgatt tttcattagt attgtatcac aaaatagaca
    19861 tacactacac taaatcattt cgaatgtcgc gcaactattt tgattatttc taacacttga
    19921 cttgcaagca agttcaatga tttaatcggc attctctcat ttgttgtatg gattttttca
    19981 taacccactc ctaaaatgac tgaaggaata ccaaatgtat taataatact gccgtctgaa
    20041 ccgccaccag aaataattgt atttgcagat aatcctaaat tacgagcact ttcttgtgca
    20101 attttaacaa ccgcttcatt atcattaatt ttaaatcctg gataactttg ctccactgta
    20161 actactgctt tcccacctaa ttctgatgca gtagtttcaa acacatcagt catatgtttg
    20221 acttgtgttt ttattctttc tggatcgtga gaacgtgcct ctgcttctaa aatgacttca
    20281 tctgcaacaa tattcgtagc tgaaccgcca tgaaacttac caatattggc agtagttatt
    20341 tcatcaactt gtcctaattt cattcgacta attgctttcg ccgcaatatt aatagcacta
    20401 acaccctctt ttggcgtact tgcatgagcc gttttgccaa aaattttagc tgaaattaac
    20461 atttgcgtcg gtgcacctac aaccgtagta ccgacatcag cacttgcatc aatagcataa
    20521 ccaaagtccg cgtccaacaa ctctgaattt aattctttag caccaattaa acctgattct
    20581 tctccaacag taatcacaaa ttgaatttgt ccatgtggga tttgttgttc ctttatcact
    20641 tgcaaaactt caagcatcgc tgctaatcct gctttatcat ctgcacctag aatagtcgta
    20701 ccatcagagt atatgtagcc gtcatctttt acaattggct ttacattaat tgcgggtaca
    20761 acagtatcca tatggctcgt caaatataat ttaggtactt cgccttcttc gatagtacta
    20821 ttcattgtac acactagatt attggcacct aatttaggat gtttagccgc ttcatcttct
    20881 ttaacatcta accctaatgc tatgaatttt tcttttaaaa taggttggat tgttgattca
    20941 ttccctgtct cagaatcgat ttgtacaagt tcaaaaaacg tattaagtaa tctttgctca
    21001 ttaatcataa tggaacccct tttatcaaaa ttttcatgta cgccaaacgg taaaaattga
    21061 tgaaataaaa tgttacagta attgacgtta cacagattta tcaggtttgt aaattgtgt
//
LOCUS       DALAGF010000041        20739 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.041, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000041 DALAGF010000000
VERSION     DALAGF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20739
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.041"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            82..825
                     /gene="sarU"
                     /locus_tag="O2692_001396"
     CDS             82..825
                     /gene="sarU"
                     /locus_tag="O2692_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HCZ4299030.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERDYIKYQWAPKYSKEFFILFMNIMYSKDFLKYRFNLTFL
                     DLSILYVISSRKNEILNLKDLFESIRFMYPQIVRSVNRLNNKGMLIKERSHADERIVL
                     IKINKIQYNTIKSIFTDTSKILKPRKFFF"
     gene            complement(1036..1902)
                     /gene="galU"
                     /locus_tag="O2692_001397"
     CDS             complement(1036..1902)
                     /gene="galU"
                     /locus_tag="O2692_001397"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCZ4299031.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKALGL"
     gene            complement(2082..4904)
                     /gene="fnbB"
                     /locus_tag="O2692_001398"
     CDS             complement(2082..4904)
                     /gene="fnbB"
                     /locus_tag="O2692_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HCZ4299032.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPKTVQAP
                     KVETSRVDLPSEKVADKETTGTQVDIAQPSNVSEIKPRMKRSTDVTAVAEKEVVEETK
                     ATGTDVTNKVEVEEGSEIVGHKQDTNVVNPHNAERVTLKYKWKFGEGIKAGDYFDFTL
                     SDNVETHGISTLRKVPEIKSTDGQVMATGEIIGERKVRYTFKEYVQEKKDLTAELSLN
                     LFIDPTTVTQKGNQNVEVKLGETTVSKIFNIQYLGGVRDNWGVTANGRIDTLNKVDGK
                     FSHFAYMKPNNQSLSSVTVTGQVTKGNKPGVNNPTVKVYKHIGSDDLAESVYAKLDDV
                     SKFEDVTDNMSLDFDTNGGYSLNFNNLDQSKNYVIKYEGYYDSNASNLEFQTHLFGYY
                     NYYYTSNLTWKNGVAFYSNNAQGDGKDKLKEPIIEHSTPIELEFKSEPPVEKHELTGT
                     IEESNDSKPIDFEYHTAVEGAEGHAEGTIETEEDSIHVDFEESTHENSKHHADVVEYE
                     EDTNPGGGQVTTESNLVEFDEDSTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDELP
                     EEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEGGQ
                     NSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNNGNQSFEEDTEKDKPKYEQ
                     GGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTLPQVSGHN
                     EGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPTEPGK
                     PIPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVVPTKKAQSKKSELPETGGEESTN
                     NGMLFGGLFSILGLALLRRNKKNHKA"
     gene            complement(5586..8642)
                     /gene="fnbA"
                     /locus_tag="O2692_001399"
     CDS             complement(5586..8642)
                     /gene="fnbA"
                     /locus_tag="O2692_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HCZ4299033.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAP
                     QTAQPANIETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAEPRTASESKPRVTRSADVAEAKEASNAKVETGTDVTSKVTVEIGSIEGHNNTNKVE
                     PHAGQRAVLKYKLKFENGLHQGDYFDFTLSNNVNTHGVSTARKVPEIKNGSVVMATGE
                     VLEGGKIRYTFTNDIEDKVDVTAELEINLFIDPKTVQTNGNQTITSTLNEEQTSKELD
                     VKYKDGIGNYYANLNGSIETFNKANNRFSHVAFIKPNNGKTTSVTVTGTLMKGSNQNG
                     NQPKVRIFEYLGNNEDIAKSVYANTTDTSKFKEVTSNMSGNLNLQNNGSYSLNIENLD
                     KTYVVHYDGEYLNGTDEVDFRTQMVGHPEQLYKYYYDRGYTLTWDNGLVLYSNKANGN
                     GKNGPIIQNNKFEYKEDTIKETLTGQYDKNLVTTVEEEYDSSTLDIDYHTAIDGGGGY
                     VDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHA
                     DVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESNLIE
                     LVDELPEEHGQAQGPVEEITENNHHISHSGLGTENGHGNYDVIEEIEENSHVDIKSEL
                     GYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGNQSFEEDTEKD
                     KPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPSYQFGGHNSVDFEEDTLP
                     KVSGQNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEV
                     PSEPETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPT
                     KKPQSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNHKA"
     gene            complement(8626..8727)
                     /locus_tag="O2692_001400"
     CDS             complement(8626..8727)
                     /locus_tag="O2692_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299034.1"
                     /translation="MLLKTEQYRLAFIKFKKLMNFAFKGRYYSEKQS"
     gene            complement(9111..10469)
                     /locus_tag="O2692_001401"
     CDS             complement(9111..10469)
                     /locus_tag="O2692_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HCZ4299035.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(10586..12139)
                     /gene="gntK"
                     /locus_tag="O2692_001402"
     CDS             complement(10586..12139)
                     /gene="gntK"
                     /locus_tag="O2692_001402"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCZ4299036.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVEALELLGISKEMLPELVPTTYVMKGM
                     KERYATLMGLNKDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIEENVTVYQEIVSIFINLSRSLTENYEQIADFQR
                     QHIAENKTQ"
     gene            complement(12163..12843)
                     /locus_tag="O2692_001403"
     CDS             complement(12163..12843)
                     /locus_tag="O2692_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCZ4299037.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(12997..13761)
                     /locus_tag="O2692_001404"
     CDS             complement(12997..13761)
                     /locus_tag="O2692_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCZ4299038.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLIALPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            complement(13926..14618)
                     /locus_tag="O2692_001405"
     CDS             complement(13926..14618)
                     /locus_tag="O2692_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCZ4299039.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(14849..15265)
                     /locus_tag="O2692_001406"
     CDS             complement(14849..15265)
                     /locus_tag="O2692_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCZ4299040.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            complement(15404..17227)
                     /locus_tag="O2692_001407"
     CDS             complement(15404..17227)
                     /locus_tag="O2692_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCZ4299041.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLQVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLAIFENIAK
                     HFELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
     gene            complement(17705..18982)
                     /locus_tag="O2692_001408"
     CDS             complement(17705..18982)
                     /locus_tag="O2692_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4299042.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            19529..20140
                     /locus_tag="O2692_001409"
     CDS             19529..20140
                     /locus_tag="O2692_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCZ4299043.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            complement(20149..20247)
                     /locus_tag="O2692_001410"
     CDS             complement(20149..20247)
                     /locus_tag="O2692_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299044.1"
                     /translation="MPNKLKKAVNHFNRWNDSLLFVYRNLKFMPLC"
     gene            complement(20237..>20739)
                     /locus_tag="O2692_001411"
     CDS             complement(20237..>20739)
                     /locus_tag="O2692_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4299045.1"
                     /translation="SILVYLPTLLNNTYDIGVGISGVLFLPITVSVILGSLFYKRFSK
                     KYNEIMILRVTITGFAIFTLLFGWLNESNVIILSVIIFILGTCVGIVPALLSTIISKR
                     FEHIKGKVLGVFNFVRYIGMTVGALLIGIISQPLVAFYFTTITIMLIVIFLYIKIVDF
                     QLKYAK"
BASE COUNT         6203 a         3947 c         3012 g         7577 t
ORIGIN      
        1 tgtctttaaa agagattgct tttatattac tttaaataat ataaagtatt gggacatcag
       61 aaaatagtag gagaatgtaa ggtggactat caaactttcg aaaaggtcaa taaattcata
      121 aatgtagaag cgtacatatt ttttctcact caagagttaa agcaacaata taaattatca
      181 ttaaaagaat tgttgatatt agcatatttt tattacaaaa atgaacacag tatttcacta
      241 aaagaaatca ttggtgacat actttacaaa caatctgatg ttgtaaagaa cattaagtca
      301 ctatctaaaa aaggatttat aaataagtct agaaacgaag cagatgaacg ccgtattttt
      361 gtttcagtta ctccaataca acgtaaaaag attgcttgtg ttattaatga gttagataaa
      421 ataattaaag gatttaataa ggaaagagac tacataaaat atcaatgggc tccaaaatat
      481 agcaaagaat tttttatact ttttatgaac attatgtact caaaagattt tttaaaatat
      541 cgatttaatt taacatttct tgatttatct atcttatatg taatatcatc tcgaaaaaat
      601 gagatactaa atttaaaaga tttgtttgaa agtattagat ttatgtatcc tcaaattgtt
      661 aggtcagtta atagattaaa taataaaggt atgctaatca aagaacgatc ccatgcagat
      721 gaaaggattg tgttaatcaa aataaataaa atacaatata acactattaa aagcatattc
      781 acagatactt ccaagattct caaaccaaga aaatttttct tttaaattta aacagattta
      841 cctcttgata aaataaataa gcaatcatac tacttctcaa tttagtataa ataaaaatac
      901 ataattaact ttcttttgtt tttatattat ttcaataccc tactatatat cacaacacat
      961 aaattaagca tgacactcat tcaatttagt tcaccatttc gtgttccaat tttactgagt
     1021 atcatgcttt taatgttata aacctaatgc tttaataaat cgtgttaatt cttctcgcat
     1081 actgtcatct ttcaatgcat attctatggt agttttaacg aagcctaatt tttctccaac
     1141 gtcataacgt tcgccttcga agtcatatgc atacacttgg ttatcattat tcatacgttc
     1201 aatcgcatct gttaactgaa tttcgttacc tgcgccttct ttttgcgttt ttaaataatc
     1261 gaaaatttca ggcgttaata cataacgtcc cataatagct aggtttgatg gtgccgtacc
     1321 ttgtgctggc ttttcaacaa actttttcac ttcatactga cgtccgtttt tagttaatgg
     1381 gtcaataatt ccataacgat gagtatctgc ttccggaact tcttggacac ctataactga
     1441 gtgccctgtt tcttcataaa cgtcaatcaa ctgtttcact gctggcactt cagattcaac
     1501 aatatcgtca cctaataata ccgcaaatgg ttcattgccg ataaattgac gcgcagaact
     1561 aatcgcatgc cctaaacctt tttgttcttt ctgccttaca taaaaaatat tcgcaagttc
     1621 cgttgaatac tgaactttct ctagtaattc agatttacct ttttctttta acaccatttc
     1681 taattctttt tgactatcaa aatgatcttc aatcgcgcgt ttgtggcgac ctgtcactat
     1741 aataatatct tcaattccag ctcttgcagc ttcttcaacg atatattgta ttgtgggttt
     1801 atctaagata ggaagcattt cctttggcat cgctttagtt gctggtaaaa atctagtccc
     1861 taaaccagca gcgggaatga ttgccttttt tatttttttc aaagttaatg tgctcctttt
     1921 cctaagtatt aaatctatgt atcaacgtca ttttaacact aattagaacg ccttcatagt
     1981 gtcattgagt atgtaattat ttcttgggaa atttgtttta attttaaaaa acaggcttac
     2041 ttcatataat ttatgaaata aacctgtcaa ttttggattg attatgcttt gtgattcttt
     2101 ttatttctgc gtaataacgc taaacctaaa atgctaaata atccgccgaa caacatgccg
     2161 ttgtttgttg attcttctcc acctgtttca ggtagttcag atttcttaga ttgtgctttt
     2221 ttagttggta ccactgcttt aaccttttca ttgatttcaa taacaggtgt tactacttta
     2281 ccttgttcca ctggtttaga aggtttttta ggttcttctt tagcaggtgg tattggttta
     2341 ccaggttcag ttggtacctc tggcgttggc ggtgttggtg tttccggctc gcttggtact
     2401 tctggtgtcg gtggtgttgg tgtttccggc tcgcttggta cttctggtgt cggtggcgtt
     2461 ggtggcacga ttggaggtgt tgtatcttct tcaatcgttt gttgaccttc attatgacca
     2521 cttacttgtg gaagtgtatc ttcttcaaag tcaacactat tgtgtccacc gaattgataa
     2581 tttggtttat ctttatttgt atcttcttca ataatttcag tgtgcttatt gaatccgtga
     2641 atatgtggca cactgtcgaa gtcgatatca atgatattac caccttgttc atacttaggt
     2701 ttgtctttct ctgtatcttc ttcgaatgat tggttaccat tattttgacc atgaatttga
     2761 ggtacactat cgaaatcgat atctacgata ttgccacctt gttcatattt cggtttatct
     2821 tcttctgtgt cttcctcaaa tgactgatta ccgctatttt ggccaccttc gtaacctaat
     2881 tcactcttaa tatccacgtg gctattttct tcgatttctt caatcacgcc ataattaccg
     2941 tgaccatttt cagttcctaa accagaatga gaaatatgat gattgttttc agtaatttcc
     3001 tcgattggtc cttgcgcttg accatgttct tcaggtagtt catctactag ttcaatcaga
     3061 ttactttcag tcgtatattc tttcgtatct tcaattgttg tatgatcgct aacagcacca
     3121 gttacaatac cttttgtaga atcttcgtca aattcaacta ggttagactc agtagtaacc
     3181 tgaccaccac ctgggtttgt atcttcttca tattcaacaa catcagcatg atgttttgaa
     3241 ttttcatgtg tcgattcttc aaagtctaca tgaatagaat cttcttcagt ttcaatggta
     3301 ccttctgcat gaccttctgc accttcaaca gctgtatgat attcaaaatc aattggctta
     3361 gaatcattac tttcttcgat tgtaccagtc aattcatgct tctccactgg cggctctgat
     3421 ttaaattcaa gttcgatagg agtactatgt tctataatag gttcctttag tttatctttg
     3481 ccgtcgcctt gagcgttatt agagtaaaat gcaacgccat ttttccaagt taaattactt
     3541 gtataataat agttataata tccaaaaagg tgtgtttgaa attctaagtt gctagcattt
     3601 gaatcataat acccttcata ttttattaca taatttttac tttggtctaa attattaaag
     3661 tttaaagaat aaccaccatt agtatcaaaa tctaaactca tattatcagt cacatcttca
     3721 aatttgctga catcatcaag ctttgcatat acgctttcag ctaaatcgtc tgaaccaatg
     3781 tgtttatata ccttaactgt tggattatta acccctggtt tatttccttt agttacttga
     3841 ccagttactg tcacagagct taacgactgg ttgttaggtt tcatgtacgc aaaatgacta
     3901 aatttcccat ctactttatt taaagtatca attcgaccat tagctgttac tccccaatta
     3961 tctctaactc cacctaaata ttgaatatta aatattttgc taaccgtagt ctcacccaat
     4021 ttaacttcaa cattttggtt acctttttgc gtcactgttg taggatcaat aaatagattt
     4081 aaagataatt cagcagttaa atctttcttt tcttgtacat attctttaaa cgtatatcta
     4141 acttttcttt ctccaattat ttctcctgtc gccataactt gaccatctgt actttttatc
     4201 tccggaactt tacgcagtgt tgagatacca tgagtttcaa cattatcgct taatgtgaaa
     4261 tcaaaataat ctcccgcctt aattccttct ccaaatttcc atttatattt caaggttact
     4321 ctttctgcgt tatgaggatt tacaacattc gtatcttgtt tatgtcctac aatttcacta
     4381 ccttcttcta cttccacttt atttgttaca tctgtacctg tcgctttagt ttcttccact
     4441 acttctttct ctgcaactgc tgtaacgtca gttgatcttt tcattcttgg tttaatttct
     4501 gagacgttac ttggttgagc tatgtcaact tgagttcctg tagtttcctt atcagcaact
     4561 ttttccgatg gcaaatcaac tcgcgaagtt tctacttttg gtgcttgcac agttttcggt
     4621 gcttcttctg ttgttacttg tgttgattgt gatggttgct cagttgatgt cgcgctgtat
     4681 gattgtgttt catctattgt attaacgtta tttgtagttg tttgtgtttc gcttgcttta
     4741 ctttcagtag ctgaactccc actttcctct actgtagtat tgttttgttc cgatgctgca
     4801 gcttcttttt cttgtcccat tccaacaacg atcattgttc ctaagaatac tgaggccgct
     4861 cccaatttgt gttttcttat gccgtatcta agattgcttt tcactataat attctccctt
     4921 aaatgcaaaa ttcatttatt tttaaaactc aataaatgca attctatatt gttcggtttt
     4981 taaaagcaat gaaaaaaagc gagttaataa aaagttaaga ttgttgttaa ctttatgtat
     5041 aatgagtttt ttattatttg aaactcacat atatattgca tacaaagctc ttgaacacct
     5101 tgatataaca ggcttgtatt ttttatactt actttttaaa ttaaattcaa attcatctaa
     5161 tttaaaacaa tatactaaac catacataat aatcgcctgt acaatgcatc attaacaagt
     5221 cactgaaacg cctttcattg tattaataac gtcactataa tttttatatc gttcggtttt
     5281 tgtttgattt taatgattat ttatacaaaa acagccgtat ttcaagccga cattttaaat
     5341 ttaactaaat ttgcatctag ttaataattg catttatcaa atttgtctta ttgatccaat
     5401 ctaatttgta ctcacaaact agtttaaaat tctaacttta tctctcagtt cgttatcaat
     5461 catcagacat aaaccaatga agcaatcaga aaacactcta attttctatt agaaatttga
     5521 tttaatataa aaaaacaggc ttacttcata taatttatga aataaacccg tcaatttttg
     5581 tttaattatg ctttgtgatt ctttttattt ctgcgtaata atgctaaacc tagaatgctg
     5641 aataatccgc cgaacaacat acctttgttt gttgattctt ctccacctgt ttcaggtagt
     5701 tcagatttct tagattgtgg ttttttagtt ggtgccactg ctttaacctt ttcattgatt
     5761 tcaataacag gtgttactac tttaccttgt tccactggtt tagaaggctt tttaggttct
     5821 tctttggcag gtggtactgg tttaccaggt tcagctggta cctctggtgt tggcggtgtt
     5881 ggagtttctg gctcactcgg cacttctggt gtcggtggtg ttggtgtttc cggctcactt
     5941 ggtacttctg gtgttggtgg cgttggtgtt tccggctcac ttggtacttc tggtgtcggt
     6001 ggcgttggtg gcacgattgg aggtgttgta tcttcttcaa tcgtttgttg accttcattt
     6061 tggccgctta cttttggaag tgtatcttct tcaaagtcaa cactattgtg tccaccgaat
     6121 tgataacttg gtttatcttt atttgtatct tcttcaataa tttcagtgtg cttattgaat
     6181 ccgtgaatat gtggcacact gtcgaagtcg atatcaatga tgttaccgcc atgttcatac
     6241 ttaggtttgt ctttttctgt atcttcctcg aatgactgat tacctttatt ttgaccatga
     6301 atttgaggta cactatcaaa atcgatatct acgatattgc caccttgttc atatttaggt
     6361 ttgtcttctt ctgtgtcttc ctcgaatgac tggttaccgc tattttggcc accttcataa
     6421 cctaattcac tcttaatatc aacgtggcta ttttcttcga tttcttcaat cacgtcataa
     6481 ttcccgtgac cattttcagt tcctaaacca gaatgagaaa tatgatgatt gttttcagta
     6541 atttcctcga ctggtccttg tgcttgacca tgctcttcag gtaattcatc cactaattca
     6601 atcagattac tttcagttgt atattctttc gtatcttcaa ctgttgtatg atcgctcact
     6661 gcgccagtta caataccttt tgtagactct tcgtcaaatt caactaagtt agactcagta
     6721 gtaacctgac caccacctgg gtttgtatct tcttcatatt caacaacatc agcgtgatgt
     6781 tttgaatttt catgtgtaga ttcttcaaag tcaattggat ttgattcctc agaggactca
     6841 gtgtatcctc caacgtgacc tgcttcgcta tccacagcag tatggtaatc gatatcaata
     6901 gctgatgaat ccgtttcttc tattgtttca atgtatccat caacatatcc acctccacca
     6961 tctatagctg tgtggtaatc aatgtcaaga gttgatgaat catattcctc ttcaacagta
     7021 gttactaaat tcttatcata ttgacctgta agagtttctt taattgtatc ttctttatat
     7081 tcaaatttat tattttgaat aatcggacca tttttcccat ttccgttcgc tttattactg
     7141 tataaaacta aaccattatc ccaagttaag gtatatcctc tatcataata atacttataa
     7201 agttgctctg gatgtcctac catttgtgtt ctaaaatcaa cttcatcagt accatttaaa
     7261 tactctccat catagtgaac aacataagtt ttatctagat tttctatatt caatgaatag
     7321 cttccattat tttgtaaatt caaattccca ctcatattac ttgtgacttc tttaaattta
     7381 gaagtatctg tcgtatttgc atatacactc ttcgctatgt cttcattatt acccaagtat
     7441 tcaaatatcc taacttttgg ttgatttcca ttctgattac tacctttcat taaagttcca
     7501 gtaacagtca cacttgtcgt tttaccatta ttaggtttaa taaatgcaac atgcgaaaat
     7561 ctattattcg ctttattaaa tgtctcaatc gatccattta aattggcata ataattccca
     7621 ataccatctt tatatttaac atctaattcc tttgaagttt gttcttcatt tagtgttgaa
     7681 gttatagttt gatttccatt agtttgtaca gttttaggat caataaataa attaatttct
     7741 agttcagccg ttacatcaac cttatcttca atatcatttg taaatgtata tctaatcttt
     7801 ccaccttcta aaacttcacc tgtcgccatt acgactgaac catttttaat ttctggtact
     7861 tttctagcag ttgatacgcc atgcgtattt acattatttg ataaagtaaa gtcaaagtag
     7921 tcaccttgat gtaaaccatt ctcaaatttc aacttatatt ttagtaccgc tcgttgtcct
     7981 gcatgaggtt ctactttatt tgtattgtta tgcccctcaa tagaaccaat ttctactgta
     8041 actttacttg ttacatctgt acccgtttcc actttcgcgt tactagcttc cttagcttcc
     8101 gctacatctg ctgatcttgt cacacgtggc ttactttctg atgccgttct tggctctgcc
     8161 acttcaactt gtgtttctgc gacttgattt tgtgtagcct ttttaggtgt taaatctact
     8221 tgtctttgat ctccgctatt gtcttgagat tgtgttgttt ccttaacttg aggtttcgct
     8281 tcttccttaa ctacctcttc tttaactgtt tctatatttg ctggttgtgc agtttgtggt
     8341 gcttgtactg cttttggtgc ttcttcagtt gttacttgtg ttgcgtttga cggttgttct
     8401 gttactgttg cgttatatga ttgagtttct tctatatgat taacgttagt tgcagttgtt
     8461 tgtgtttcac ttgttttatt atcagtagct gaattcccat tttcttctac tgtagttgtc
     8521 ttttgttctg atgctgcagc ttctttgtct tgtcccatcc caacaacgat cattgttcct
     8581 aagaatactg atgctgctcc caatttatgt tttctaatgc cgtacctaag attgtttttc
     8641 actataatat ctccctttaa atgcaaaatt cattaatttt ttaaacttaa taaatgcaag
     8701 tctatattgt tcggttttta aaagcaatac aaaaaagcga gttaatgaaa agttaagatt
     8761 gtcattaatt ttatgttaat tgataatttt attatttgaa atatacctat aaattgtatt
     8821 caagtcatca gaaacccttg tcacacaagg cttgtatttt ttatacttat ttttttaaat
     8881 taaattcatc attatctaat ttaaaacaat atactaaacg tttcataatt atcgcctgta
     8941 caatacgcac aaaaacatgt cttgaaacgc ctttcatcac tctaaaatac ccaatatact
     9001 ttttatatcg ttcggattct gagtatttca gacgattttc tgcataaaaa taaacgtgtt
     9061 tcaaggcaat atattgcaat tacctaaaaa cacgtttact taatatttag ttaaacaaat
     9121 aagctaatga ataaaatgaa gatgatacct gaaacggaaa taatcgtttc taataatgac
     9181 catgttaaga atgtttcttt tacagttaaa ccaaaatatt ctttaaacat ccaaaatcct
     9241 gcgtcattta catgagacaa aatcacacta cctgcaccta tcgcaagtac aactaatgca
     9301 acatttacat ctgatgattg taataatggt aagacaatac ctgtagttga aatcgcagct
     9361 actgtagccg aacctaatgc gatacgtagc acagctgcaa caatccatgc tagtaaaatc
     9421 ggagacatct ctgtaccttc aaacatttta gcaattgtat ttccgacacc gccgtcaatt
     9481 aatacttgtt taaatgtacc gccaccgcca ataatcaata acatcattcc gattggataa
     9541 atcgcattcg tcactgattc cataatatga ttcatcttac gctttctcat taatcccatc
     9601 gtaacgattg caaataatac tgctattagc atggctgtcc ctgctgttcc tatcatataa
     9661 atgatagatt caaatagatt tgtaggtttg tcatgcccag ttacaagttg cgttatcgta
     9721 gacactaaca ttaatatgac tggtaatgtt gctgttaata aactcatacc aaatcctggc
     9781 atctcttgat ccgtaaattc tttttgtgca cctaacgctg aaatatcgcc ttctcgtgta
     9841 tacgcagacg gaatcatttt ttgtgcaact ttgttaaata taggccctgc aatgagtgta
     9901 actggaatgg caataatcat accatacagt aatacatctc caacatttgc ctttaattct
     9961 tttgcgatga ctaccggtcc tggatgtggt ggtaaaaagc catgtgtcac tgataaagct
    10021 gttaccatag gtagtcctag ttttaacact gaaacatttg cgcgttttgc tactgtaaat
    10081 actaatggaa tcagtaagac taaacctact tcaaagaaca atgcaatacc gacgataaat
    10141 gctgcaacaa gcattgccca ttgtacatgt ttttgaccaa atttttgaat caacgtgtct
    10201 gcgattcgag ttgcaccacc accatcagca agcaatttcc caagtatggc acctaaaccg
    10261 aatatcagtg caatgtggcc gagcgtactg cccattcctt tctcaatcgt ctccataatt
    10321 ttagtcaatg gtatacctag cattaacgct gtaatcatcg atgtgataat taatgaaata
    10381 aatgtattta atttaaaccc aataattaat actaataaaa taacgatacc taaaacaaca
    10441 ctgattaacg gccatatttc gttaaacatg acattcccct ctttctcttt tcaatagaat
    10501 gtaacaccgt cgtcgcgcac gtattccaca taatatatag tagtcatggc acaacaactt
    10561 tatctaagat gcttgagtga cgtatttatt gtgttttatt ttcagcgata tgttggcgtt
    10621 gaaaatctgc aatttgttca taattctctg ttaaagaacg acttaaattg ataaaaatgg
    10681 atacgatctc ttggtaaaca gtgacatttt cttcaatcgg cgtatgattg tttgtggcac
    10741 cgaccatcga tgaaacgatt gaaaaatctt caatgtcacc tacagcttta agtccgagca
    10801 cgcaggcacc taagcatgaa ctttcataac tttcaggaac cactaactct gtgtcaaata
    10861 tatctgacat catttgacgc catacttcac ttttcgcaaa accacctgtt gcttttatca
    10921 tcttaggtgt ttcattcatt acttcaataa gcgcaagata gacggtatac aaattgtaaa
    10981 gaacaccttc taatgcagcg cgaatcatat gttctttttt atgagataaa gttaaaccga
    11041 agaatgaacc tcttgcattt gcgttccaaa gcggcgcacg ttctcctgct aaatagggat
    11101 ggaatattaa accatctgca cctggtttaa cacgctttgc aatttgagtt aagacatcat
    11161 aaggatcaac accgagacgt ttcgcagttt cgacttcact cgctagcaac tcgtcgcgca
    11221 accatctcaa tacgacacca ccattattta caggacctcc gatgacgtag tggtcctctg
    11281 ttaagacata acaaaatatt ctacctttgt aatcagtacg cggtttatct atcacagtac
    11341 gaatcgcccc agatgtaccg attgtgacag caacttctcc tttaccaaca ctattgacac
    11401 ctaaattaga aaggacccca tcactcgcac caataacaaa cggtgtatct ttattaagcc
    11461 ccattaatgt tgcataacgt tctttcatac ctttcatcac atacgttgtt ggaactaatt
    11521 ccggcaacat ttccttggaa atacccagca gttctaatgc ctcaacatcc caatctaatg
    11581 tttctaaatt aaacatccct gttgcggaag ccattgaata atcaatgata tatgtatcaa
    11641 ataaatgata gaaaatgtat gttttaatat ctgcaaactt agcagtacgt tgaaatacat
    11701 cttgccattc atgtttcatc caaaaaatct tcgctaatgg cgacatagga tgaatcggtg
    11761 tgcctgttcg ctggtaaatc gcattgccat catgcacttc atttattact gttgcatatt
    11821 ttgcagcgcg gttatctgcc caagtaatat tatttgttaa tctttgatgt tgctgatcca
    11881 tcgcaatcaa gctatgcatt tgcgcactaa atgacacaaa cttaatgtcg tctttattaa
    11941 ctttggattc tctcataaca tatttaatag tcattagtac tgcatcaaat aattcatctg
    12001 ggttttcttc tgagacatca acgtttggtg tgtgtaaatc atagcctatt tgatgtttca
    12061 tgataaaagt tccattttca tcatataaga ctgacttggt actcgtcgtt ccaatgtcga
    12121 caccaatcat atatttcatg ataaatcctt ctttctttca ttttaattca accaaaatcc
    12181 ttcaatatct ttaccaacat cgtcgaaatt taaatgaaac gcttctttca aaatttgact
    12241 gtcgtattgt tccactgcat caataaacac ttgatgatta tgatgtatgc gttcaaaatc
    12301 ttgcgggttc tgttgcatac gttgtcgcat ggatgttaaa acgagtgcca tcattactgg
    12361 ttttaaatga ctccaaaatg agtttaaata ttgatgatta gatgctttga ttaatgtttc
    12421 atgaaattca aagtcatgct tcgtaaatga ttctgcatcc tcaaatttta ctgccacttt
    12481 catcatttca agttgtttct tcatttcttt tacgatgggt agtcgctctt gatttttaac
    12541 tcttgaaaat gcaaatgact ctaacatcag tcgcaaatca tacatttctt tcttttcttg
    12601 ttccccaaac ggcaacacat gtgcacccat tctttctaat tggatgagtt gattttgttg
    12661 caataattta aatgcatctc gaattggcga acgactcaca ttaaattgct ttgccatttg
    12721 attttcagtg agtaacgtac cttcagctat gtgaccattc acaatgccta agcgtaattc
    12781 tgccgcgata ccttctccag ttgtcatacc ttccaaccat ttctctggat atccatacat
    12841 catcaaagtc actccttcat tacacgacat acttgtatac aagtatgtta atatagttat
    12901 tatgagtttg caagcgcttt ctttacgagc actaaaatag tgaccacccc ttttcgattt
    12961 aaatttaaag gaaatggtca ctatcacacg aatgatttaa ttgttatgtt gtatgtggga
    13021 tatttctaat tgttctgtac tcatatgcgc tttaggtact tcaatgcaat aatgcgtttc
    13081 atgacagttt ggacattcga atcgacgtgt tgtcgctgta tgtttcgctt tgataactgc
    13141 ccacaaagat ggtgagaata tatgctggca gttaggacat aaataggcaa ccttttgttg
    13201 gtaataaaaa gtaacaccaa tgccataacc aatcataaat ggtaaagcaa ttaaaaacgg
    13261 ccatttattt ttcatcaaaa ttgcacttat aatgctagaa tattgaatta ttcctataat
    13321 accagcacta atccaaatgt tacgacgaat acttttcatt tcagctgatt tactcatgac
    13381 atgctctatg tcttttaagt gtgtgattgg agacgtcgac gcttcattta cgtaatattg
    13441 aacattttta attttgttta ataccgcttg ttgctgttta acttgttggt taatttcttg
    13501 ttgtttcata gttagtaaag tattgagcgt cttcaaagta ccttcacctt ttagcaacat
    13561 atctatatcg cttaacgcac aacctaaatc tttaagcaat aagattaact ctaatgtttg
    13621 tcgctgttgt tctgtataca cacgacgctt tccttctgta aatccttgtg gtttcaaaat
    13681 acctttgcga tcataatatt gaatcgttcg tgttgtcaca ttgcataatt ttgcgagttc
    13741 tccagtcgaa tagttagaca tagattccac ctcctataat taccatagtt gatgacccga
    13801 cgtcacgagc aagtacaatt tccacatttt aaagaaattt attatactag gcgtcttatt
    13861 tttatgattt cgtaccatgt tgatttacaa actcactcaa actaagtaac acacctacta
    13921 aacatctact ctgttatttc agaatgaatt tgttgtaatt tatcttcaac ttcagtaatc
    13981 tctgtcgcac attctttcag taaatctcga tacttttccg tctctgcatt gtttttataa
    14041 cgtattttat gttctaaact tgcccacata tccataccta tcgttctaat ttgaatttca
    14101 acaggcaata cctcaacgcg ttctgctaaa aagactggaa tagatacaac gatatgtaaa
    14161 ctgcggtaac cattttcttt agggtgctga atataatctt tacgttttat caattgtacg
    14221 tcttcttgtt taagcaacat ctcttcaatc aaataaatat catctaagta attacatact
    14281 acgcgaattc cggcaatatc cagtatatgt tctttggcag tttctgcgct aatctgtaat
    14341 cctttacgat taagcttttc tattaaacta cgcatttctt tcacacgtcg ctccatatga
    14401 tgtataggat tgtgtttata aatgtgattg aaattgtcat ctaaaatact cagtttagtg
    14461 cttatttcct ttagtgctga cgaataaata tgatctaact ctacgaaacc taataacgta
    14521 tcaaatgctt catcaccatt ttcaaattta cttaagctat ttttaaaatc atgtcgcaaa
    14581 tcctctaaat atagtgatgg ttttcgatct acatacattt ttatactaac ctccgatata
    14641 taatcatctt tattgtacct aacattttat taagataata ctaatacact atcgaacttt
    14701 gggctgatac tgaaacagca caaagaaact caaacaattc gaattatgta tcaaaacctt
    14761 caattacagg aagtgatttt aaaatgattt aacgcaaaaa aacacctgtt accgttatat
    14821 aggtacaagt gcttaattga tagagatgtt atacgtcttt aaatgattcc acaacttttg
    14881 gatgtggacc atccataagc ggttctcttt gtcgaacgcc accactttgg ttgccaattg
    14941 attcgctatc aaagtacgat ttatcttgat ttgattgttc ttgaatatgt tcttcattat
    15001 cagttgttgc atattcacta tccacctctg ttttttccat tgttgctgta tggaattgta
    15061 cgaagttctc ttcttctaat gctttgattt cttctttcga taacgctcga taccaatctt
    15121 tcgctttttt cgccgcaata ggaacaacat ctttttcaag atatccttct tttaacattg
    15181 cattcgcaat ttctatcgct ttttgccgtt taattacttc aaattctttc catttttcgg
    15241 ggtaaccttt cattgtaaag ggcatttcct taacctccaa tatgttataa ttcatgttat
    15301 ataccaccat aattataaaa tgaaacatat taaacacatt tttttagact cccgtcataa
    15361 taacttcaga caaacgttag tctgacctga taaatattta attttaaacg ttcttgtctc
    15421 tctcacgaaa accattgtgt tgaatattca tcattgctga ttgaatataa ttatactgtg
    15481 gtaaatctgg taatatcttt atttctaatt ctgtatttaa ttcgaaatgc ttagcgatgt
    15541 tttcaaatat tgctaaatac tcgcccatta attgttcatt tattgtaagt ctatcttcat
    15601 tagccatggc tctatttaac ataaagctaa tttcttctaa tgcgaataag ctaggataat
    15661 aattttgaat caatgtctta tcactaaata attcaccatt agctgcatta taaacttgcg
    15721 tcatgttatt taatttcaca gataggttca tagccgtgtt tttctgatac acatcatctt
    15781 tatattgatt ttcagaaaat aaataatgaa agagcgttgc ttctttacga acaacttcag
    15841 ccattacatt aggaagcaat gcggatgcgg tacgttgtcc aaggacaaat aaacctatga
    15901 ttgcaatcac aatacccatt gcaacgtcaa taactcttgg aaacgcaatg ttaattgtta
    15961 aattctgtga tgctaatccg ttcattaaaa taacttgtat tgtaataaaa actactgcaa
    16021 tcgcataatt tgctcccacc aatgcttcag taaacaatgc tgcaatgccc attaaaatta
    16081 ctgcaacagg tgttggtatt gaaaacaaca atatgactga aagtactaac acacctaaaa
    16141 tagtacctaa acctcgtgcc atacctctct cgattgcatg tatagttgat gttcctagta
    16201 atattgtatg tgcagataac ggtatccagt atgctttttc aaaatcaaac attaacgcaa
    16261 taaatatcgc tatcatcata atcgctgtat atctcaatgt atttctaaaa acaatagagt
    16321 ctaatgttaa attttgataa atgcgtttac tatataaagg ttttcgcatg tctgcttcat
    16381 acgcaatacg cgcttcattt gcatgcacca tttcatcaat tttcaatata tggttgaata
    16441 aattttgaaa ttcctctgtt actgtcactt cttttcgcca atatcttaca ttttcctcac
    16501 tattatctag ttgtgcaatg atatgatcca tcatttcaat taactcatct ggcaatggtc
    16561 gaatttgttt agcgttcaac tctaacagtt cagaataaat cccttgggca gatgtgtgta
    16621 ataataataa tttttggaaa cgtctactta atttatcgtt acttgaagta gaagtgatta
    16681 ataatttatc agatgcttta aaactatcca ccgctgtttt tgctacttgt aaaaaagcag
    16741 atttatcatt ataagtgtgt aacaacttag atatgagttt aaaatcattt tgaattgctt
    16801 gttcttcagc tttgttttta gaaaatacga tcgtgattaa caccatcata gtggcaagta
    16861 taccgcctac taaaatcgca aagcctctat ataatgcttc ttcgggagct ataggtaagt
    16921 taatgggtag actgaatgtc acaatgaaga atgtcgatga cggtccagct atttttaagg
    16981 cagtaaatat ataaaatggt ataaccgtaa caattaataa tgtcattcca aaaacgagtg
    17041 gcgttttggc tgttagcgta ccaagcatca tacatattgc aaacgctaaa ttacaaatta
    17101 ttacagttcg cagcttagat cgcgacggac ctttaaaaac ataaatatgg gctagtgttc
    17161 cggttgcaac taataatcca aattggaaat taccacataa gtaaccgatt attgcaggaa
    17221 taatcattaa cagtccctgt cttacacctt tataaaagtc taagttaagt cgattgattt
    17281 ttatgagtga tgtcaaaaag atgtcatttc gacttctcct ttccttgtca aaatatttcc
    17341 ataatctttt tattagcata gttaaaatga atttgttgct ataaatttta attacgcaac
    17401 atatgtcatt ataacacctt atgctcaatt atctaaattt aaaaactttt aaagtccaat
    17461 taaaattaat ttctaatgtt atttaacatc gttgaacctt tggtcgccca cctttgcgct
    17521 agttaaaatt tatatgtact tttacaagcg ttacctgact aatgttttaa aaccttattt
    17581 ctttacatat atcatttcaa taacttcatt ttcatcttaa accatatctg ttttaaaaag
    17641 tttcaatttc aacgcaaaaa aagcaataag ccccaactat gtgaaagctt actgcttgtt
    17701 taaattattt ttctattgct ttatcatcac taacaattaa aggttgatct ggtttcacat
    17761 agaaccacat aatcacagcg attactgctg gaacgattaa caattggaat gtcattgtcc
    17821 aacccaaatg ttctacgaat agacctgcta atagcggact gattaatgca cctatattac
    17881 cccataagtt catccaccct gatactgtac cagagaagtt acgtcctaag tcagttgctg
    17941 cagcccaact catacccatt gaaataccga taccaccaag acctagtgat aaccagaaaa
    18001 tacttacata taaatttcct gtgcgtacag caaagataat tgaaactgca aacacaataa
    18061 atcctgcaat tgcaattaca cctctagcta cgaattttga acgacctaat cctaatacac
    18121 ggtcagaaat tgcacctgct gataagatta agaagaacat taataaccaa ggtaatgaac
    18181 taatgctcat ttctttaaag ttaacgtgga atacttctgt taaatacgtc ggtaaccaaa
    18241 ttaagaataa tgtaatgata aattgtacaa caaagtattg aattgcaatt gcatagaagc
    18301 taaaacgttt aaagaaatca ttccatggtg gtgatgactt ttcagtagct acgatatcac
    18361 gattttccat aatgaaacgt ttctccgctt cattaaccat tctatgttgc tcaggtaagt
    18421 ctttggcaat aatcgcccat aatacagcca ttaaaatacc tactgcacca aaaatgtaaa
    18481 atactgcttg ccagttaaat gcgttaacaa tagcaattgt aactattggt gctaatacag
    18541 gtccgaaata tgatcctgct aataatgcac ttgatgctct acctttttca tttttagaga
    18601 accaaaatga attaaacaca gcattagaag ggtacattgg cgcctcacct acaccaaata
    18661 agaatctcac taaataaatt aaaccgtggt tcttaatcat acccgttaag attgtaaatg
    18721 cactccacca aactaatgca attgtaatca tcttacgtgg accgaatttt tcagccaaca
    18781 tccccgatgg tacttgcatt aatgcataac ctagagagaa gaatgatgcc aataaaccaa
    18841 attgtggttt tgtcatccct aaatcatcca tcatttgctt agcgatatat gaaatgtttg
    18901 ctctatccat atacgcaata accccaataa agaagaacgc aagcgcgaac atccacctta
    18961 cgttagttcg tttttccttc ataactattc ctacttccct aattaatttt ttaaaactta
    19021 ccctgaaacg ttaaaatttt cggacatctg gcatgtccac ccctactttg tcacaaaatt
    19081 gtaccaaagt tattttttat aatattatca gacatggaaa gcgtttacaa tagaatctat
    19141 aaaaatttta ttaaattccg cttaactttt gctattaaaa cgatataaag gtacttttag
    19201 ttacatagta gcctatcgtt tgttaattaa aaatactgca atcctagtgt tttaaaacgt
    19261 atattgcagg ctttcttaca tattcgacat tcattttaaa ttgcatataa ctacattaac
    19321 cattcattgt gcagcgcctg ttgtttagat gatcatttat tgactttttg ctcttatagc
    19381 aagcaatttt ttgatttatt gcttataata cattttaaga caaatgtttg tttcaacatc
    19441 gcatgcgaca tcattaatta ttcatttgaa tttatccagt ttctatttgt ctgcaattgc
    19501 tgaaaattaa aggagcttat ttttaataat ggaacaaatt atcactgaat ttattagccg
    19561 ttttgggtat gcagccattt ttatattaat tttattagaa aatgtactac ctatcgtacc
    19621 atcggaaatt attctgacat ttgctggtct tatgtctgtt aaatcacatt tatcaatttt
    19681 aactttattt attattgcaa ctatcgcatc gtttataggg ctgttaattt tatattatat
    19741 ctgccgtttg atttcagaag aacgtctata tcgttttatt gatcgacacg gtaagtggat
    19801 taaattgaaa agtaaggatt taaagcgagc aaatgattgg tttaaaaagt atggcgtatg
    19861 ggctgtattt atctgtcgtt tcatacctgt attacgtgta ttgattacca ttccagctgg
    19921 tgtaaaccgc atgaatgttg tgacatttac cgttatttca ttaataggta ctacaatctg
    19981 gaatttcggt ttaattttac taggacgcac tttgagcgat agctttggta tgttgatgac
    20041 tggtcttcat acatattcac gtattatgta tgttgtcatc attatcgcag tcatttactt
    20101 tgctatacgt tacattggca aacgtaagag agtaaaataa tattgttgtt agcataaagg
    20161 catgaatttt aaatttctat aaacaaaaag cagtgaatca ttccatctat tgaaatgatt
    20221 cactgctttt ttaagtttat ttggcatact ttagctgaaa gtcaactatc tttatataaa
    20281 gaaatattac tattaacatg atagttatag ttgtgaagta aaaggctacc aacggctgag
    20341 aaatgatacc aattaataat gcaccgacag tcattccaat atatctcaca aaattaaata
    20401 cacctagcac tttccctttt atatgttcaa accttttaga aattatagta gacagcaaag
    20461 ctggcacaat accaacacaa gtacctaata taaatataat tactgataat ataattacat
    20521 ttgattcgtt taaccatcca aacaataacg taaatatagc aaatccagtt atagtaacac
    20581 gaagtatcat tatttcatta tatttttttg aaaatcgctt ataaaataaa cttcctaata
    20641 taacactaac agtaataggt aaaaacaaaa ctcctgatat tccaacgcct atatcatagg
    20701 tattatttaa taatgttggt aaataaacta atattgaaa
//
LOCUS       DALAGF010000042        20189 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.042, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000042 DALAGF010000000
VERSION     DALAGF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20189
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.042"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            138..629
                     /gene="lspA"
                     /locus_tag="O2692_001412"
     CDS             138..629
                     /gene="lspA"
                     /locus_tag="O2692_001412"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCZ4299046.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
     gene            629..1546
                     /locus_tag="O2692_001413"
     CDS             629..1546
                     /locus_tag="O2692_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCZ4299047.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNNDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRSGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTSEYIERHAELPQDFEDLLDTIRKRDA"
     gene            1945..2472
                     /gene="pyrR"
                     /locus_tag="O2692_001414"
     CDS             1945..2472
                     /gene="pyrR"
                     /locus_tag="O2692_001414"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCZ4299048.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            2690..3997
                     /locus_tag="O2692_001415"
     CDS             2690..3997
                     /locus_tag="O2692_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCZ4299049.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            4025..4906
                     /locus_tag="O2692_001416"
     CDS             4025..4906
                     /locus_tag="O2692_001416"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCZ4299050.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGGSLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            4924..6198
                     /locus_tag="O2692_001417"
     CDS             4924..6198
                     /locus_tag="O2692_001417"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCZ4299051.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            6200..7300
                     /gene="carA"
                     /locus_tag="O2692_001418"
     CDS             6200..7300
                     /gene="carA"
                     /locus_tag="O2692_001418"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCZ4299052.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            7293..10466
                     /gene="carB"
                     /locus_tag="O2692_001419"
     CDS             7293..10466
                     /gene="carB"
                     /locus_tag="O2692_001419"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCZ4299053.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            10576..11268
                     /gene="pyrF"
                     /locus_tag="O2692_001420"
     CDS             10576..11268
                     /gene="pyrF"
                     /locus_tag="O2692_001420"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCZ4299054.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQDTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQNDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            11268..11879
                     /locus_tag="O2692_001421"
     CDS             11268..11879
                     /locus_tag="O2692_001421"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCZ4299055.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKLAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            11909..12121
                     /locus_tag="O2692_001422"
     CDS             11909..12121
                     /locus_tag="O2692_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299056.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQEQI"
     gene            12558..12959
                     /locus_tag="O2692_001423"
     CDS             12558..12959
                     /locus_tag="O2692_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4299057.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(13222..14919)
                     /locus_tag="O2692_001424"
     CDS             complement(13222..14919)
                     /locus_tag="O2692_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HCZ4299058.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFITS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLYKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS"
     gene            15194..15817
                     /gene="gmk"
                     /locus_tag="O2692_001425"
     CDS             15194..15817
                     /gene="gmk"
                     /locus_tag="O2692_001425"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCZ4299059.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLEHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            15817..16035
                     /locus_tag="O2692_001426"
     CDS             15817..16035
                     /locus_tag="O2692_001426"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCZ4299060.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            16251..17450
                     /gene="coaBC"
                     /locus_tag="O2692_001427"
     CDS             16251..17450
                     /gene="coaBC"
                     /locus_tag="O2692_001427"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCZ4299061.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            17450..19858
                     /gene="priA"
                     /locus_tag="O2692_001428"
     CDS             17450..19858
                     /gene="priA"
                     /locus_tag="O2692_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCZ4299062.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMVASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
BASE COUNT         7147 a         2927 c         3809 g         6306 t
ORIGIN      
        1 aatgtcaaaa catagtagtt tatcaagtat tgagttagta acattagatt taatgtaata
       61 tgcttacttt ttttattagc aggtgtaagc tataatataa agagttgtct catggacgat
      121 tgattggagg aacgaaaatg cacaaaaaat attttattgg cacttccatt ttaatagcag
      181 tatttgtcgt tatatttgac caagttacta aatatattat agctactaca atgaaaattg
      241 gagattcatt tgaagtgata ccgcactttt taaacataac atcacatcga aataatggtg
      301 ctgcatgggg aatattgagt ggaaaaatga catttttctt tattattacc attattatat
      361 taatagcctt agtatatttc tttattaaag atgctcaata taatttgttt atgcaagttg
      421 ctattagttt actttttgca ggtgcacttg gaaactttat tgatagaatt ttaacaggag
      481 aagttgttga ctttattgat acaaatattt ttggttatga ttttccaata tttaatatcg
      541 cagattcaag tttaacaatt ggtgtaatat taattattat tgccttatta aaggatactt
      601 ccaataaaaa ggagaaggag gttaagtaat ggagacttat gaatttaaca ttacagataa
      661 agaacaaaca ggtatgcgtg tagataagtt gctgcctgaa ttaaataatg attggtctcg
      721 taaccagata caagattgga ttaaagcagg tttagtcgtt gcaaacgata aagttgttaa
      781 atctaattat aaagtgaaac ttaatgatca tatagttgtc actgaaaaag aagtggttga
      841 agctgatatt ctacctgaaa atttaaattt agatatttat tatgaagatg acgatgttgc
      901 agttgtatat aaaccgaaag gcatggtagt tcatccatca ccagggcatt ataccaatac
      961 attagttaat ggtttaatgt atcaaattaa aaatttgtca ggtattaatg gagaaattcg
     1021 ttcaggtatt gttcaccgta tagatatgga tacttctggt ttattaatgg ttgctaaaaa
     1081 tgatattgct catcgtgggc ttgtagaaca attaatggat aaatctgtta aaagaaaata
     1141 tatcgcttta gttcacggga atattcctca tgattacggt acaatcgatg cgccaattgg
     1201 tagaaacaaa aatgatcgtc aatctatggc tgttgttgat gatggtaagg aagcagtgac
     1261 acattttaac gtactagaac attttaaaga ttatacgctt gttgaatgtc aacttgaaac
     1321 aggacgtacg catcaaatcc gtgttcacat gaaatatatt ggcttcccat tagttggtga
     1381 tccaaagtat ggaccgaaaa agacattgga tattggtggt caagctctac atgctggact
     1441 tattggattc gaacatccag taacaagtga atatattgaa agacatgctg aattaccaca
     1501 agactttgaa gatttattag atacaattcg aaaaagagat gcataattgt gtcgtgctat
     1561 aattacgata acattattat gtaaactata agtgtttatc gttttcgttt ttttaaatga
     1621 aaacacatta taaaatagat taaggttaac atttttactg agtatggtta atttttcaat
     1681 atagattgac aaagcgcctg attagtatta tccttataac agtttaagta caacataaag
     1741 agtctttaat tgagcgtcca gagagacgtc aaagacatga aaatatagat atatactaaa
     1801 atgatcataa cactttcaca tatgcaagaa taagtagtaa tactacgatt cttctatgta
     1861 aataggctga ttgtatatac taaacctcat gtcgatatta aagacatgag gttttttgtt
     1921 tctaaatttt gaaagggtga gaatatgtct gaacgtatca taatggatga tgccgcaata
     1981 caacgtacag tgacgagaat cgctcacgaa attttggagt ataacaaagg tactgataat
     2041 ttaattcttt taggtatcaa aacaagaggt gaatatttag cgaatcgtat acaagataaa
     2101 attcatcaaa ttgagcaaca acgtatacct actggaacaa ttgatattac atactttaga
     2161 gatgatatag agcacatgtc atcacttacg acaaaagacg caatagacat cgacacagat
     2221 attacagata aagtagtcat cattattgac gatgtgctgt atactggtcg aacggttcgt
     2281 gcttcacttg atgctatttt gctaaatgct agacctatta aaattggttt agctgctttg
     2341 gttgatcgag gacatcgtga gttaccaatt cgagcagatt ttgttggtaa aaatatacct
     2401 acttctaaag aggaaacggt aagtgtctat ttagaagaaa tggatcaaag aaatgcagtt
     2461 ataattaaat aatacccctt ttaattcagt acgagagact gaaaaaggtg ggggagttga
     2521 atgataaata agttttcata tgcttgttta tctaataata ccctcatgtt cgacaactat
     2581 aagtcaattt ctatggacga aaatgacttg tagcaacttg tcacactttt tgtctcttta
     2641 catatactga tgtaaagaga tttttttatt atcggaggaa atgaatatta tgcaaaatga
     2701 tgaaatgttt gaacgaacag taaaacccgt actagatgta aatgaaaaac cacaaccagc
     2761 gcaatgggca tttttaagct tacaacattt atttgcgatg tttggcgcaa cagtactagt
     2821 accattctta acaggactac caatatcagc agcgttacta gcttcgggaa tcggtacatt
     2881 actttatatc ttaataacga aggcgcaaat accagcatac ttgggatcta gctttgcatt
     2941 tatcacgcca attatcacgg gattaagtac gcatagctta ggagacatgc ttgtagcatt
     3001 attcatgagt ggtgttatgt acgtcatcat cgggattcta atcaaattaa gtgggacagc
     3061 atggttaatg aaattattac caccagttgt tgttggacca gtcatcatgg taattggttt
     3121 aagcttagcg cctactgcag tcaatatggc aatgtatgaa aatcctggag atatgaaagg
     3181 ttacaatatc agtttcttaa ttgttgcaat gataaccttg cttgtaacaa tagtcgttca
     3241 aggatttttt aaaggattcc tatctttaat tccagttctt gtaggtatta tcgtaggtta
     3301 tgtggtagcg atttttatgg ggattgtgaa atttgatgca attatgtcag caaaatggat
     3361 agatttccct catatttatc tgccatttaa agattatgta ccttcatttc acttaggact
     3421 tgtacttgta atgattccga ttgtgttcgt aacagtaagt gaacatattg ggcaccaaat
     3481 ggtattgaat aaaatcgtag gtagaaactt ctttgaaaag ccaggacttg ataaatcaat
     3541 cattggtgat ggtgtttcta caatgtttgc cagtattatt ggtggaccac caagtacaac
     3601 atacggtgaa aatatcggtg tattagcgat taccagaata tacagtattt acgtcattgg
     3661 tggtgcagca gttatagcaa ttgttttagc attcattggt aagttcactg cattaatttc
     3721 ttctatacct acaccagtta tgggaggagt atctatatta cttttcggta ttattgcagc
     3781 aagtggctta agaatgttag ttgaaagcaa agtagatttt gcgaacaatc gaaatttagt
     3841 tatagcttct gtaattttag ttgtaggtat cggtaattta gtatttaact taaaagaaat
     3901 tggtatcaac cttcaaattg aggggatggc attagctgca ctttcaggaa ttattttgaa
     3961 cttaatctta cctaaagaga aaaaacaaaa caattaagat ttacaaatta aggagggcgc
     4021 ttttatgaat catttattat caatggaaca tttatctaca gatcaaatat acaaacttat
     4081 ccaaaaggca agtcaattta aatctggtga acgtcaacta ccaaactttg aagggaaata
     4141 tgtcgcaaat ttattctttg aaaattctac tcgaacaaaa tgtagttttg aaatggcaga
     4201 acttaagcta gggttaaaaa cgattagctt tgaaacatca acatcatctg tttcaaaagg
     4261 tggatcttta tatgacacat gtaaaacttt agaaagtatt ggctgtgatt tattagtcat
     4321 tagacatccg tttaataact actatgaaaa attagcgaat attaacatcc caattgcgaa
     4381 tgctggtgat ggtagtggac aacatccaac acaaagttta cttgatttaa tgacgatata
     4441 tgaagaatat ggatattttg aaggcttgaa tgtattgatt tgtggagaca ttaaaaattc
     4501 acgtgtcgca cgtagtaatt accatagttt aaaagcatta ggtgcaaacg taatgtttaa
     4561 tagcccaaat gcttggattg atgattcttt agaagcacct tatgtaaata tagatgatgt
     4621 tatagaaaca gtagatatag ttatgttatt aagaattcaa catgaaaggc atgggcttgc
     4681 agaagaaact agatttgcag cagatgatta tcatcaaaag catggcttaa atgaagtacg
     4741 ctataacaaa ttacaagaac atgctattgt tatgcatccg gcacctgtga atagaggagt
     4801 agaaatacaa agcgatttag tagaagcttc aaaatcaaga atttttaagc aaatggaaaa
     4861 tggcgtttac ttaagaatgg cagtcattga tgaattatta aaataggtaa ggggacgaaa
     4921 atgatgaaat taattaaaaa cggtaaagta ttacaaaatg gcgaattaca acaagcagat
     4981 attttaattg atggtaaggt aattaaacaa attgcacctg caattgaacc aagcaatggt
     5041 gttgacatca tagatgcgaa aggtcacttt gtgtcacctg gatttgtcga tgttcatgtt
     5101 catttacgtg aacctggtgg tgaatataaa gagacaattg aaactggtac taaagctgct
     5161 gctagaggcg gatttacaac tgtatgtcca atgcctaaca caagaccggt accagattct
     5221 gtagaacatt ttgaagcttt acaaaaatta atcgatgaca atgctcaagt acgtgtatta
     5281 ccttatgctt caattacaac acgtcaatta ggtaaagaat tggttgattt cccagcacta
     5341 gtaaaagaag gtgcctttgc gtttacagat gacggtgtag gagtacaaac tgcaagcatg
     5401 atgtatgaag gcatgattga agctgcaaaa gtaaacaaag ccatcgtagc acactgtgaa
     5461 gataattcat taatctatgg tggtgcaatg catgaaggga aacgcagtaa agagttaggt
     5521 ataccaggta ttccaaacat ttgtgaatct gttcaaatcg caagagatgt actattagct
     5581 gaagcagcag gttgtcatta tcatgtatgt catgtttcta ctaaagaaag tgttagagtc
     5641 attcgtgacg ctaaacgcgc aggcattcat gttacagctg aagttacacc acaccattta
     5701 ttgttaacag aagatgatat tcctggtaat aatgccattt ataaaatgaa tccaccattg
     5761 agaagtactg aagatagaga ggctttgtta gaagggttac tagacggtac aattgactgt
     5821 atcgcaacag accatgcacc acatgcacgt gatgaaaaag cacaaccaat ggaaaaagca
     5881 ccattcggaa ttgttggtag tgaaacagca ttcccattat tatatacgca ttttgtaaaa
     5941 aatggtgatt ggacattaca acaattagta gattacttaa caattaaacc atgtgagaca
     6001 tttaatttag aatacggcac attaaaagaa aatggttatg cagatttaac aatcattgat
     6061 ttagatagtg aacaagaaat taaaggagaa gatttcttat caaaagcaga taatacacca
     6121 tttatcggct ataaagttta tggaaatccg atcttaacaa tggttgaagg cgaagttaaa
     6181 tttgaggggg ataaataata tgcaaagcaa acgttatcta gtgttagaag acggttcttt
     6241 ttacgaaggc taccgtttag gatctgataa cttaactgta ggagaaattg tatttaatac
     6301 agcgatgaca ggttatcaag aaactatttc agatccatca tatacaggtc agatcattac
     6361 ttttacgtat ccattaatcg gtaattatgg tatcaataga gacgattttg aatcattagt
     6421 acctacatta aacggtattg tagtgaaaga agcgagtgcg catccaagta attttagaca
     6481 gcaaaagaca cttcatgacg ttttagaatt gcatcaaatt ccagggattg caggtgttga
     6541 tacaagaagt attacgcgta aaattcgaca acacggtgtg ttaaaagctg gttttactga
     6601 tcgaaaagaa gatattgatc aacttgtcaa acatttacaa caagtagaat tacctaaaaa
     6661 cgaagtagaa atcgtttcga ctaaaacacc gtatgtttcg acaggtaagg atctaagtgt
     6721 cgtacttgta gactttggta agaagcaaaa tattgttcga gaattaaacg tcagaggttg
     6781 taacgtcaca gttgtaccat atacaactac tgccgaagaa attttagcaa tggctccaga
     6841 tggcgttatg ctatcaaacg gaccaggtaa tcctgaagtt gtagaatgtg cgattccaat
     6901 gattcaagga attttaggga aaattccgtt ctttggtatc tgtctaggac atcaactttt
     6961 tgcattatct caaggagcaa gctcatttaa aatgaagttt ggtcatcgtg gtgcgaacca
     7021 tccagttaaa aatttagaga ctggaaaagt tgatattacg agtcaaaacc atggatatgc
     7081 aatagatata gattcgttaa aaagtactga tttagaagtt actcatcttg cattaaatga
     7141 tggtactgta gaaggtttaa aacataaaac attaccagca ttttctgttc aataccatcc
     7201 tgaagcaaat ccaggaccgt cagattcaaa ctatctattt gatgattttg tagcaatgat
     7261 gactaatttt aaggaaaagg agcgtcatat caatgcctaa acgtaatgat atcaaaacaa
     7321 ttttagtaat agggtctggg ccaattatca taggtcaagc agctgaattt gattatgctg
     7381 gaacacaagc atgtctagct ttaaaagaag agggatatcg agttattctt gtaaattcaa
     7441 atccagcgac aatcatgact gataaggaaa ttgcggataa agtatatatc gaaccgttaa
     7501 ctcatgattt tatagcgcga attatacgta aagagcaacc tgacgcttta cttccaactt
     7561 taggtggtca aacaggttta aacatggcga ttcaactaca cgaaagtggt gtgcttcaag
     7621 ataataacgt ccaattatta ggaactgagc taacatcaat tcaacaagca gaagaccgtg
     7681 aaatgtttag aacattaatg aatgatttaa acgttcctgt accagagagt gacattgtaa
     7741 atacagtaga gcaagccttt aaattcaaag agcaagtggg atacccgcta attgttagac
     7801 cggcatttac gatgggtggt accggaggcg gtatttgtca taatgatgaa gaattacatg
     7861 aaatcgtctc aaatggtctt cattatagtc cagcaacgca atgtttatta gaaaaatcta
     7921 tcgcaggttt taaagaaatc gaatacgaag taatgcgtga taaaaacgat aatgccatcg
     7981 ttgtatgtaa catggaaaat attgatccag ttggtattca tacaggcgat tcaattgttg
     8041 tggctcctag tcaaacatta tcagatgttg agtatcaaat gttacgtgat gtttcattaa
     8101 aagttattcg agctttaggt atcgaaggtg gttgtaatgt tcaattagca ttagatcccc
     8161 attcattcga ttattatatt atagaagtaa atccgcgtgt atcacgttca tcagcgttag
     8221 cttcaaaagc aacaggatat cctattgcaa aattagctgc taaaatcgcg gttggtctaa
     8281 cattagatga aatgttaaat ccaattacag gaacatctta tgcagcgttt gaaccaactt
     8341 tagactatgt gatttcaaaa ataccaagat ttccttttga taaatttgaa aaaggagaac
     8401 gagagcttgg cacacaaatg aaagcaacag gtgaagttat ggccattggt cgaacttacg
     8461 aagaatcatt gttaaaagca attcgatcac ttgagtatgg tgtgcatcac ttaggattac
     8521 caaatggtga aagcttcgat cttgattata ttaaagaacg tatttcacac caagatgatg
     8581 aacgattatt tttcatcggc gaagcaatta gaagaggcac aacattagaa gaaattcata
     8641 atatgactca gattgattac ttcttcttac acaagttcca aaacattatt gatattgagc
     8701 atcaattaaa agagcatcaa ggtgatttag aatatcttaa atatgcaaaa gattatggat
     8761 ttagtgataa aacaatagcg catcgcttta atatgacgga agaagaagta tatcaattgc
     8821 gtatggaaaa tgatattaaa cctgtttaca agatggttga tacttgcgca gctgaatttg
     8881 aatcttcaac accatattat tatggtacat acgaaactga aaatgaatcc atagttactg
     8941 acaaagaaaa aatcttagta ttaggctctg gaccaattcg aatcggccaa ggtgtagaat
     9001 ttgactatgc gacagttcac gccgtttggg caattcaaaa agcagggtac gaagcgataa
     9061 ttgtgaataa caatccagaa acagtttcaa cagacttctc aatttctgac aaattatact
     9121 ttgaaccttt aactgaagaa gatgtgatga atatcattaa tttagaaaaa cctaaaggtg
     9181 tcgttgtaca atttggagga caaacagcga ttaatttagc agacaaattg gctaaacatg
     9241 gtgttaaaat acttggtact tcactagaaa atctaaatcg tgctgaagat agaaaagaat
     9301 ttgaagcact attaagaaaa attaacgtgc cacagccaca agggaaaaca gctacatcac
     9361 ctgaggaagc attagcgaat gctgcagaaa tcggatatcc ggttgtagta agaccttctt
     9421 atgtattagg tggtcgcgca atggaaattg tagacaatga caaagagtta gaaaactata
     9481 tgacccaggc tgtaaaagcg agtccggaac atccggtact agtcgataga tatttaactg
     9541 gtaaagaaat tgaagttgat gcgatttgtg atggagaaac ggtcattatt ccaggaatca
     9601 tggaacatat tgaacgtgct ggtgtgcata gtggtgactc aatcgctgta tatccaccac
     9661 aaactttgac agaagacgag ttagcaacac ttgaggacta tactataaaa ttagctaaag
     9721 gtttaaacat cattggctta atcaacattc aattcgttat agctcacgat ggtgtgtatg
     9781 ttttagaagt aaatccacgt tctagtagaa cggtaccatt cttaagtaaa attactgata
     9841 ttccaatggc acaattagct atgcgagcaa tcattgggga aaaactaaca gatatgggtt
     9901 atcaagaagg ggttcaacca tatgctgagg gtgtctttgt gaaagcacca gtatttagtt
     9961 ttaataaatt gaaaaatgtt gatattactt taggacctga aatgaagtca acaggtgaag
    10021 tgatggggaa agatactaca ttagaaaagg cgttattcaa agggttaaca ggtagtggcg
    10081 ttgaagttaa agatcacggt acagtattaa tgaccgtcag tgacaaagat aaagaggaag
    10141 ttgttaaatt ggcacaacgc ttaaatgaag ttggctataa aattttagca acgtctggaa
    10201 cagctaataa attagctgag tatgacatac ctgcagaagt agtaggcaaa attggtggcg
    10261 aaaatgattt attaacacgt attcaaaatg gtgatgttca aatcgttata aatacaatga
    10321 ctaaaggtaa agaagtagaa agggatggct tccaaattag acgtactaca gttgaaaatg
    10381 gtattccatg tttgacatct ttagatacag ctaatgcctt aacgaatgta attgaaagta
    10441 tgacatttac aatgcgtcaa atgtaaatca atcaaactgt atcggtgggg ctgtaattaa
    10501 ccatttactt aaagaagttt atattacagc ctcattattt taatgaattt cttaatataa
    10561 agggagacat atatgatgaa agatttacca attattgcat tagattttga atcaaaagaa
    10621 aaagtaaatc aatttttaga tttatttgat gaatcattat tcgtaaaagt aggtatggaa
    10681 cttttttatc aagaaggtcc tcaattaatt aatgagataa aagaaagagg ccatgatgta
    10741 tttttagatt taaaactgca tgatattcct aatacagttg gtaaggcgat ggaaggacta
    10801 gctaaattga atgttgatct ggtaaatgtt catgctgctg gtggcgtaaa aatgatgtct
    10861 gaggccatta aaggattaag aaaacataat caagatacaa aaattattgc agtaacacag
    10921 cttacgtcaa caacagaaga catgttacga cacgaacaaa atatacaaac atcgattgaa
    10981 gaggccgttt taaattatgc caagttagca aatgcagctg gtttagatgg cgttgtttgt
    11041 tcacctcttg aaagtcgtat gttgactgaa aagttaggta catcattttt aaaagtaaca
    11101 ccaggtatta gacctaaagg tgcatctcaa aatgaccaac accgtattac gacaccggaa
    11161 gaagcaagac agcttggttc gacgcatatt gtagtcggta gaccgattac acaaagtgac
    11221 aatccagtcg aaagttatca taaaattaaa gaaagttggt tagtataatg gctaaagaaa
    11281 ttgcaaaatc attattagat attgaagctg taacattatc accaaatgat ttatatacat
    11341 ggagttcagg tattaaatca ccgatttact gtgataaccg tgttacgtta ggttatcctt
    11401 tagttcgagg cgcaatccgc gatggtttaa ttaacttaat taaagaacac tttcctgaag
    11461 tagaagttat ttctggtact gcaacagctg gtattccaca tgcagctttt attgctgaaa
    11521 aattaaaatt accaatgaat tatgttcgtt catcaaataa gagtcatggt aagcaaaatc
    11581 aaatcgaagg tgctaaaagt gaaggtaaaa aagtagttgt gatagaagat ttaatttcga
    11641 cagggggatc ttcagtcaca gcagttgaag ccttaaaact agcaggtgca gaagtattag
    11701 gtgttgtagc tatctttact tacggtttga aaaaagcaga tgatacattt agcaatattc
    11761 aactaccttt ttacacttta agtgattaca atgaattaat tgaagtagct gaaaatgaag
    11821 gtaaaatttc tagtgaagat atccaaacat tagttgaatg gagagacaac ttagcataat
    11881 atagacacta gaaggaggaa ttcaacaaat gaatgacaaa acatctaatg atttatatgg
    11941 gaagataaaa cattgtaacg aatttatcaa tcattcaaat gattccaatc tatctagtag
    12001 tcacgatgtc gacgaaagtt caacgaagca aaaacatata aaaaataaaa caactataga
    12061 tcataatgat gatttattta aacatgtaaa ggatatatta cgtaaacaag aacaaattta
    12121 aaaacaaaaa agctatagtg tttaatatca ggggctgctt aaccgcatcc gaatattaca
    12181 aacactatag cttttattat ataaaaaatt tattagcgga taattaactt tattaaaatc
    12241 caaccaatta agaatacgag taataggact aatactggaa tcacataatg taacataacg
    12301 tccctccttt aacttaattt taattgtaat caaatttgac aataagtcaa aacattaata
    12361 cctatgataa gtatcattta ttaacatatg tatcatattt ttaatcttgc gtaattttta
    12421 tcgttaacta tgtgatttaa tcaacaggcc aataagacgt ttgtcattcg tgcacataac
    12481 ggtacgacgc gcttttgtca ttttaattat gttaagataa tagtagttat agaagttcaa
    12541 tctataggag gcatagcatg gatattccaa aaatcacgac atttttaatg tttaataacc
    12601 aagctgaaga agctgttaaa ctatacacaa gcttatttga agatagtgag attataacaa
    12661 tggctaagta tggtgaaaat ggacctggtg atcccgggac tgtacaacac tcaatattta
    12721 cattaaatgg acaagtattc atggcgattg atgctaatag tggcacagaa ttaccaatga
    12781 atcctgcgat ttcattattt gttacagtaa aagatactat tgaaatggaa cgactattta
    12841 atggattaaa agatgaaggt gccattttaa tgccaaaaac gaatatgcca ccatacagag
    12901 agtttgcttg ggttcaagat aagtttggag taagttttca attagcatta cctgagtaaa
    12961 aggattcgca cagcgttgaa tgataaagaa acactttttc ttatgcatgc gttttaccta
    13021 tgtatttcta ttttaagtac acattagcat tttagtttca ctcattttta aaatcacaaa
    13081 gataatttgt aacttaaatg atgatacgtt atttaaaaac acgatacttc gtttcaatga
    13141 acgcattaaa taataaataa acacctcacc ataagaaagg actactttct tttgtgaggt
    13201 gtttatttgt cgacaacttg attatgattg tttcattttt tgaataagtt cataatcagg
    13261 tgtagcatac aaagtttttt gattgtcata tgttacaaac ccaggctttg cacctgatgg
    13321 tttatgcaca tttttaatta atgtataatc aacaggtatt tgtccagaat taccagcttt
    13381 tgaaaagtat cctgctaaca tagccgcttc cttgattgtc gtatcacttg gtgcatcatt
    13441 aaatataacg acatgtgaac caggaatatc ttttgtgtgt aaccatgtgt gagttttttt
    13501 agctttttta tttgttaaat aatcattttg cttgttattc ttaccaacat atatatcgtc
    13561 gccatcagtt gatacataat gttgtaattg aatctgcgct ttctttttct tagtttgatt
    13621 tttacgctgt ttcataaagc cttgttctgc taattcatct ctaatttcat caatgtcatg
    13681 gacagaaata tgatgtaatt gttgttcgat tgttgaaaaa taatctatat tgtctttcgt
    13741 caattgaatt tgatgttgta attcacgttc tctcgttttc atacgattat attgtttata
    13801 ataatattga gcatttgctg atggggattt tgtaggattt aaaggaatga caacttcttc
    13861 attcgtataa taattcaatg ccgtcacttc tttatcgcct tgcttaattc gatatatatt
    13921 agcagtgatc aattcaccat ataactgttc agtatcttta tttttagact gctcatattc
    13981 ttcaatcaac ttcgctaatt tattttgata tttgtacaac tgctgttgaa caaatcgaac
    14041 taaatcattc gcacgttgtt taacgcgttc acgttcgcca cgcgcatcat aaaaacgatc
    14101 aagcaaatca tttaatgaat cgtatgtaac tgtatcatca ttaaattgat ttaactttat
    14161 aaaatagaaa tcctctttac ctgtttcatg atttttatga aaaataggag taggtggtaa
    14221 cttggtttct gccattactt cgtcaaatgc ttctggtaat gttgatgaag taataaattg
    14281 acgacgacta acgatttcat tcgtaattaa agggctaaat ccttcaaact gattcaataa
    14341 ttgtttagca atattacctg cgttaaaatc gatatatttc aacacctctg cacctgtaat
    14401 atcatacgga tttattttgt gctgagtagg tggtgcttca taattaaatc ctggcattac
    14461 tgtacgatag tgattcgtat ttggtgttaa gtgtttaaat ccttcaatta ttttgcgatt
    14521 ttcatctact aaaattaagt tactatgttt acccataatc tcaaggatga cagtgcggta
    14581 aatagtatcg ccaatttcat ctttactctt tatatcgatt tcaatgcgac gatcattacc
    14641 aatttgctta atcgattcga taataccacc ttctaagtgt tttctaaaaa cacgcgcaaa
    14701 catgggtgga ttaaatggat tatcatattt tttagtagtc aattgtaatc ttgaaaagtt
    14761 tggatggatt gacaataaca attgatggtt ttgtctattt tgacgtacaa ccattagtat
    14821 cgtgtcatta tcaggttgat tgattttgtg aacgcgtcct gttgttaaaa attgtagaga
    14881 ctcaaccatt ttctttgtaa ataagccatc ataagccata atttatcttc caccttcttc
    14941 atatcattcg tttcatttta acatgattgt aacttttcag tatagttatt gaaccataca
    15001 ttatgccata cattttacat tatctaaaag tttgcaattc aatttattta attaaaatta
    15061 atcgtgcaat ataacgatat tgttagactt aatagaaatt atggcatgca atttcaaata
    15121 tgctatacaa tataaagaac aatgtgatat catatttaaa taatagaaga ttagcttaga
    15181 gaggtcgtaa ggcatggata atgaaaaagg attgttaatc gttttatcag gaccatctgg
    15241 agtaggtaaa ggtactgtta gaaaacgaat atttgaagat ccaagtacat catataagta
    15301 ttctatttca atgacaacac gtcaaatgcg tgaaggtgaa gttgatggcg tagattactt
    15361 ttttaaaact agggatgcgt ttgaagcttt aatcaaagat gaccaattta tagaatatgc
    15421 tgaatatgta ggcaactatt atggtacacc agttcaatat gttaaagata caatggacga
    15481 aggtcatgat gtatttttag aaattgaagt agaaggtgca aagcaagtta gaaagaaatt
    15541 tccagatgcg ctatttattt tcttagcacc tccaagttta gaacacttga gagagcgatt
    15601 agtaggtaga ggaacagaat ctgatgagaa aatacaaagt cgtattaacg aagcgcgtaa
    15661 agaagttgaa atgatgaatt tatacgatta cgttgtagtt aatgatgaag tagaacttgc
    15721 gaagaataga attcaatgta ttgtagaagc tgagcactta aaaagagagc gcgtagaagc
    15781 taagtataga aaaatgattt tggaggctaa aaaataatgt taaatccacc tttaaaccaa
    15841 ttaacgtcac aaattaaatc aaagtattta attgcaacaa ctgcagcgaa aagagcgcgt
    15901 gaaattgatg aacaacctga aactgaatta ttaagtgaat atcattcatt taaaccagtt
    15961 ggtagagcgt tagaagaaat tgctgacggt aaaattcgcc ctgttatttc aagtgattat
    16021 tatggtaaag aatagtttcg accattaaaa atatgtgaag ctagacgtac atcaaatacg
    16081 tcaaggcata tccaaatcaa acgcacctat gtaggtgtgt ttttttagtt tatccaatga
    16141 ttgatgttat aataatacta aatttgtatc tataaaaaag taatgagcat ttgtgcgcat
    16201 atgatgatgt aaagcgtaaa tgtcatagtg atatttaagg gagattgaat atgaagaaaa
    16261 tattattagc cgttacaggt ggcattgcgg catataaagc aattgatttg acaagtaagt
    16321 taacacaatc tgggtatgaa gttcgcgtta tgttaacgaa tcacgcacaa aaatttgtga
    16381 caccattagc atttcaagca ataagtcgaa atgctgttta tacagatact tttatagaag
    16441 aaaatccttc agaaatacag catattgcat taggtgattg ggcagatgca atcattgttg
    16501 cacctgcaac ggcaaataca attgcaaaat tgagtgtagg tattgctgat gatttggtga
    16561 catcaacgtt gctagcaaca gagacaccga aatttattgc gcctgctatg aatgtgcata
    16621 tgtatgaaaa taaacgtacg cagcaaaata ttaatatttt aaaagaagat gggtatcatt
    16681 ttatcgaacc aggaagcgga tttctagcat gtggttatgt tgctaaagga cgtatggaag
    16741 aaccgcttca aatcgtttct gtaattgatg ctcattttca aaatagtaat cgtttagcta
    16801 atagttcatt tcaagataaa cgcgcattgg ttacagcagg accaactatt gaagttatcg
    16861 atccagtcag atttgtatcc aatcgttctt ctggaaaaat gggctatgca atagctgaag
    16921 cattgcgaaa tcgaggagct atcgtgacgt tagttgctgg tcctacaaca ctagaggatc
    16981 caaaagatat tgaagttatt catgttcaaa gtgctgaaga aatgtttgaa caagtgacaa
    17041 gccgatttga cgaacaagat attgttgtaa aagcagcagc cgtatctgac tatacaccgg
    17101 ttgatgtatt agaacataag atgaaaaagc aggatggtga tttgtcagta tcttttaaac
    17161 gtactaaaga cattcttaaa tatttaggtg aacataaaac atcacagtat ttaataggct
    17221 ttgcagcaga gactgaagat attgaaaatt atgcacaaca aaaattacgc aagaaaaatg
    17281 cagatgtgat tatttcaaat aatgttgggg atatgtctat cggatttagt tctgatgata
    17341 atgaattgac aatgcatttt aaaaataatg aaaaagtaaa tatcaagaaa ggaaaaaaag
    17401 tagtattagc tgcacaaatt ttagatgaac tagaaactag gtggcaataa tgatagcgaa
    17461 agtcatagtc gatgtcgcgt cgaagagcgt tgactataaa tttgattata taattcccga
    17521 acaactcgaa tctgtcatcc aacctggtgt gcgtgtaatt gtaccttttg gaccaagaac
    17581 gattcaaggt tatgtaatgg aagtaacagc agaacctgat gcacaacttg acgtttcgaa
    17641 gttaaaaaaa atcatagaag tgaaagatat acaaccagaa ttaacatcag aattaatagc
    17701 tttaagtgag tggatgggtt caactcatgt cattaaacgt atttctatgc tagaagtgat
    17761 gcttccgagt gctattaaag cgaagtataa aaaagcattt aagatgaaag atgacataga
    17821 gctaccttca gctttattac aaaaatttga taagcatggt tactattatt ataaagatgc
    17881 gcaaaaaaat aatgatattc aattgcttat gaagttgtta aaagatgata tcgttgaaga
    17941 aaaaacgatt ctcacacaaa atataactaa aaaaaccaag cgtgctgttc gtgtcattga
    18001 agggtatcat cctgatgaag tattagctaa gttggagaaa gttattaaac aatacgattt
    18061 gtatgcttac ttgtctgaag aacaacataa aacaatattt ttaactgata ttgaggatat
    18121 gggcttttca aaatccagtt tagatggact tatcaaaaaa ggttatgttg aaaaatatga
    18181 cgcggttgtt gaaagagacc catttaaaga tcgtgttttc gaacaagaat caaaacagca
    18241 attaacagaa gaccaatata aagcatatga agcgattaaa gctaaaattg taagccaaga
    18301 gcaagaaaca tttttacttc atggtgtgac gggatcaggt aaaacagaag tatatttaca
    18361 aacgatagaa gatgttttaa gccaaggaaa acaggcgatg atgttagttc ctgaaatcgc
    18421 tctaacaccg caaatggttt tacgcttcaa acgtcgattt ggtgatgacg ttgctgtatt
    18481 acattctggc ttatctaatg gggaacgtta tgatgaatgg caaaaaatta gggatggtcg
    18541 tgcgagagta agtgttggtg caaggtcaag tgtgttcgca cctttcaaaa atttagggtt
    18601 aatcatcatt gatgaagaac atgaatctac atataaacaa gaagattatc cgagatatca
    18661 cgctagagaa attgcccaat ggcgaagtga atatcatcac tgtccagtca ttttaggaag
    18721 tgcaacacca tgtcttgaaa gttatgcacg agctgaaaaa ggcgtttatc atttgctatc
    18781 attaccaaac agagtgaacc aacaagcttt acctgaaatt gatatagtag acatgcgtga
    18841 agaattgagt gaaggtaatc ggtcaatgtt ttcaaaagat ttacgtgaag ccatacaatt
    18901 aagattagat cgacaggaac aagttgtttt atttttaaat cgacgtggtt atgcatcgtt
    18961 tatgttatgt cgggattgtg gatatgtacc gcaatgtcca aactgtgata tttcattaac
    19021 gtatcataaa acgacagact tattaaaatg tcactattgt ggttaccaag agacgccacc
    19081 gaatcaatgt ccaaattgtg agagtgaaca cattcgacaa gtaggtactg gtactcagaa
    19141 agttgaagaa ctattgcaac aagaatttga agatgcgcgc ataattagga tggatgtaga
    19201 tacaacctca aagaaaggtg cacatgaaaa gttattgact gaattcgaaa aaggtaacgg
    19261 tgacatttta ctaggtactc agatgattgc gaaaggatta gattatccaa atattacttt
    19321 agttggtgtg ctgaatgcag atacaatgtt aaatttacct gattttcggg cgagcgaacg
    19381 tacttatcaa ctattaacgc aagtggctgg tagagctggt cgtcatgaaa aggcaggtca
    19441 agtcatcatt caaacgtata atccagatca ttattcaata ttggatgttc aaaaaaatga
    19501 ttatttaaca ttttatcgtc aggaaatgga atatcgtaaa ttaggaaagt atccaccgta
    19561 ttattatttg attaatttca caatctcaca taaagaaatg aagaaggtta tggtagcatc
    19621 gcagcatgtt cataaaattt tattacagca tttaacagaa aaagcgcttg tactaggtcc
    19681 atctccggca gcacttgcga gaatcaacaa tgaatttaga ttccaaattt tagtgaaata
    19741 taaaagtgaa cctggattat tacaagccat acagttttta gatgactatt accatgaaaa
    19801 atttataaaa gaaaaattag cattgaagat tgatattgat ccacagatga tgatgtaaca
    19861 ttactaatta ttagaaacaa gttcagtatg tacgagtatt tgaaccagtg tttgaatatt
    19921 tactttaagt acagaaaaag ggcagaatat acaactgtta actatttaaa ttagcagttt
    19981 atattctgtc tttttatatg gctttataac ttacgtgatt ttggtttgat aaggaattta
    20041 ttagtatttt catttacgaa ttctgatttt aatgcaagcg ccatacttac tgatgaataa
    20101 aagtactaat gattaaacaa actattttta aatggcaaat tgaaatagtg cgaattttaa
    20161 acttataaat taatggctat aaactattt
//
LOCUS       DALAGF010000043        19211 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.043, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000043 DALAGF010000000
VERSION     DALAGF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19211
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.043"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            371..745
                     /locus_tag="O2692_001429"
     CDS             371..745
                     /locus_tag="O2692_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCZ4299063.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
     gene            898..1296
                     /locus_tag="O2692_001430"
     CDS             898..1296
                     /locus_tag="O2692_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCZ4299064.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLSLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLISGIKLLTNNKPKDEISDLS"
     gene            complement(1512..2336)
                     /locus_tag="O2692_001431"
     CDS             complement(1512..2336)
                     /locus_tag="O2692_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCZ4299065.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(2573..3880)
                     /gene="brnQ"
                     /locus_tag="O2692_001432"
     CDS             complement(2573..3880)
                     /gene="brnQ"
                     /locus_tag="O2692_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCZ4299066.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRLFGSFGNLV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT"
     gene            4464..5354
                     /locus_tag="O2692_001433"
     CDS             4464..5354
                     /locus_tag="O2692_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HCZ4299067.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            5603..6652
                     /locus_tag="O2692_001434"
     CDS             5603..6652
                     /locus_tag="O2692_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCZ4299068.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASSVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTVILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            6665..7342
                     /locus_tag="O2692_001435"
     CDS             6665..7342
                     /locus_tag="O2692_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4299069.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKQRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            7550..8581
                     /locus_tag="O2692_001436"
     CDS             7550..8581
                     /locus_tag="O2692_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCZ4299070.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPTTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            8924..10042
                     /locus_tag="O2692_001437"
     CDS             8924..10042
                     /locus_tag="O2692_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCZ4299071.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            10017..10940
                     /locus_tag="O2692_001438"
     CDS             10017..10940
                     /locus_tag="O2692_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCZ4299072.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVIAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            10951..12171
                     /locus_tag="O2692_001439"
     CDS             10951..12171
                     /locus_tag="O2692_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCZ4299073.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKKIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINISKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     TDVEIDNLTKSTETKTLNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(12276..13808)
                     /locus_tag="O2692_001440"
     CDS             complement(12276..13808)
                     /locus_tag="O2692_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HCZ4299074.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVVNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(13848..14729)
                     /locus_tag="O2692_001441"
     CDS             complement(13848..14729)
                     /locus_tag="O2692_001441"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCZ4299075.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            14887..15747
                     /locus_tag="O2692_001442"
     CDS             14887..15747
                     /locus_tag="O2692_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCZ4299076.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(16025..16825)
                     /locus_tag="O2692_001443"
     CDS             complement(16025..16825)
                     /locus_tag="O2692_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCZ4299077.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            16965..17633
                     /locus_tag="O2692_001444"
     CDS             16965..17633
                     /locus_tag="O2692_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCZ4299078.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     IMED"
     gene            complement(17760..19073)
                     /locus_tag="O2692_001445"
     CDS             complement(17760..19073)
                     /locus_tag="O2692_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCZ4299079.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPISNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
BASE COUNT         6801 a         2921 c         3547 g         5942 t
ORIGIN      
        1 gaaatgataa aaaagtatat actattaata tcttttttac tataattata aatagttaaa
       61 tatttgggaa ttaaataaat atataggagg caatttttag agatacatag caatgtaaag
      121 gtatcaaata gttatttcat aatttattat gatgacataa ttttcagtag atagttgtaa
      181 attaacactg aaatagatgt ttatattaag aagactttaa tatcataatt aatcaaaatc
      241 ttgcgtcaaa catttaaacg aattttaagt tgataacaat tggcaaaaca ttgttcaaac
      301 atcacaatga taaagcatat tatcagtatt gtagtgtgtg gaaaatgaca gccatctaag
      361 gagaaaaatg atgaaaagaa tattggtagt atttttaatg ttagcaatta tattggcagg
      421 ttgttctaat aaaggtgaaa agtatcaaaa agatattgat aaagtgtaca aagaacagaa
      481 tcaaatgaat aaaattgcct cgaaagtaca aaacactatt aaaacagaca ttaaacaaga
      541 agacagtaat acacatgttt ataaagatgg taaagtcatt gttattggta ttcaattata
      601 taaagatcgt gaaaaaatgt attatttcgc atatgaaata aaagatggta aggcagagat
      661 taatagagaa atagacccaa ttaagtatat gaaagaccat aaagcagatt atgaagatga
      721 aaatgtagaa gtggaaaaag attaacatag gctttcttta aaacttttaa ccagtttcaa
      781 aaagtaaatg agctttagcg acaataattt ttaaaataaa cacgatataa atttaaacat
      841 ttatatataa gaacacaaac gcaagtttgt aaataataat aataaaggag taagacaatg
      901 gaaaaatcga tcaaaataat gacaataata ggaattgttg ttcagggttt agcaacggta
      961 tttagtttac tattgatggt tttagcagca tcaggtgtaa tgactacaga tgtgtcaaca
     1021 acagttaatg gtgaggttga cccagttgat gcagaaacag cagcagcaat tttcactgta
     1081 ttattcttat ccctattcat atttggaatc atttcaatta ttttaggtgc aatcggtatg
     1141 tttaaagcat ctaaaaacaa aaaaatgagt ggtatattgt tgattattgg agctgtaata
     1201 agtggtaaca taattacatt tgctttatgg ttaatcagtg gtattaaact acttactaat
     1261 aacaagccta aagatgaaat aagcgactta tcataaacat cgtatattga aatttcaaga
     1321 tttcttaagt aattaaataa agtgctctct tagaagtaga ttttcagtca ataactgctt
     1381 ttaagagagc attttgtatt tgataatgct catagcatcg gataaaaatc attgacgaac
     1441 tttaaaaaaa tacgttagac aatgcacttc taaatattaa aggcatcgac taacgcattg
     1501 ttcaaatata gttaattatt tttataaaat tgatgatgat cattcaagta agcatagaat
     1561 aaacctataa tgagtccgcc tccaatatag ttaccgataa aagccgcagc gatattcgaa
     1621 atagctggta tgaagtgcaa tgtatcaact tgataaatta aaccacccat aaataagcaa
     1681 ctgttgtaaa cgacgtgttc ataacccata aaggcgaata tggtaacacc gaacatcatg
     1741 acaaacattt ttgcgagtac atcgtcaatt tgcatggcaa taactaatga aatattgata
     1801 aagaaattgg cgaagatcgc tttcattaat atacttacaa aaccagtaga caacgtttta
     1861 tgctctataa ctgctgataa ctgatttaac atatctggcg tcattacatt tgaaaaacgc
     1921 atgaaactaa ataaaatagc agcacctaaa atatttcctg caaagcataa taaaaatatt
     1981 ttcaatactc tagttggttt aattacttta taatacaggc ctacagtaaa gtacatgaag
     2041 ttactggtta gtagttcgga gtttgtaaat aaaatgagta ctaacgcaaa gctgaatgta
     2101 atggcactgg ccatattcac aatgcctggc ggtaaatctg gttcgtgtgt tgctttaact
     2161 gataatacga agaccgtaat aatcccgata ataaatcctg ccatcatcgc gcgtaataaa
     2221 taacgtttta aataaacgct ttgtaatata tcttttgttc ttatcgtttc gactacgtta
     2281 tttacccagt cgtccccata aaaaatctta tcccatttaa tatgtttctc cttcacaatg
     2341 acatcctcac ttttcatttg ttaatcagag tatactatga attgccttat ttgtgaataa
     2401 tttcacaaaa ttaattttaa aaactaatat ttttgaataa cagctaggat tttttaacaa
     2461 atcatttatt ttaaaataat caatagacag acggtatcac tgaaaagtca tgaatttcat
     2521 ataaaaaagc ctagtaacga tgggtcaatc attactaggc agtgagcatt tattaagttg
     2581 tcgcttgttt cggacggcgt ataaatacat cgattatgaa accgataata gcaaagagca
     2641 tgaatggtac aagccaagct aaatcgatat ctgctaaagg taacatcata aacgatttca
     2701 aaataacacc gtgtaataag ttgaaactat ttagtatttg taaaattgaa ataatcaatg
     2761 taataacagt tgcgagtcga taggcccaac tgaatctgaa tgtgctaaac atgttagcaa
     2821 atgatatgag tacaagtgca atcgacacgg gatatattaa agtcaataat gggacagcaa
     2881 tttttaaaat catttctaaa ccaagtgttg taaataagaa ccctatgata gagaaaataa
     2941 gtgcgaatat tttataagaa aacttaggta cgtgtttctt agtaaatgtg gcgcaagcat
     3001 tgacgagtcc tatacatgtt gttaggcatg caaggataac cgtcattcca aatacgaggt
     3061 taccgaacga accaaataat cgtaatgagt tgtacgtcaa tatatctgta ccatctttaa
     3121 agtttcctgg agctgttgat gccccaacgt atgcaagtgc aaagtaaatc attccaagta
     3181 atatggctgc aataagacct gaaaagcaga catattttaa aattttcatg cgatctgtga
     3241 ggcctttaaa cttatagcca ttgacaatga ctacggaaaa agctaacgca gcaacaagat
     3301 ccattgtaaa atagccttcc aaacttcctg aaatgaaagg atgtgttata tatttatcct
     3361 taggtgcact tagtgcagat tcagggttga aaatgacagc aatacttaat agagcgacca
     3421 ttaatagtaa taacggtgtt aataatttac ctaaattatc aacgattttc gatggattta
     3481 aactaatcca gtaaacgatg gcaaaaaaga ttgctgcgaa tataattaaa gtccattggt
     3541 tatgcacagg taaaatgtgt cttgtaccaa tttcgtacgc gacatttgca gcacgtggaa
     3601 taccgtaaaa tgctccgata gacatgtaaa tcacgacagc aaaaataaac ccgaaccatg
     3661 gatgtatacg attgcctaca ctttcaacac cttcatcata aaatgcaaca acaataacag
     3721 taataaaggg gagtaatatg cctgtaaggg caaagcctag cataccaatc cacatatttt
     3781 gacccgctgt atggccaagc atgggcggga atattaaatt tccggctcca aaaaatagtg
     3841 aaaataacat gaggcccgaa ataataactt gttttttcaa tgtaatttcc ttcctaaaac
     3901 tagatattca taataattta aaaaatctga aaaataaaaa cgttcttact ttatctttaa
     3961 atgacaatac tatgattcta ttattttata aaaaagattg caataataac gtcttgctat
     4021 tattggataa tgacaattaa agtgtcgttt ttatgaatta aaataggtga aagtaagttg
     4081 gaaatacgtt ggagcgttgt gatttttatg agaaattgta tgagtgagga ctgctattta
     4141 taaaaggtaa actatggatg agtttatggt tgttccaata ggtaaataag agatagcaca
     4201 ctgtacacat aatgatacgt ggatgagtga atatgtctga taggaaggcc tagtcactca
     4261 aatgaattta agtttattac gtgataaatc acaaatctct ctcatgtgat aggtctccca
     4321 ttaaatcatg cttatatgaa atgttcacat atttgttagc ttttcaagaa ataatattaa
     4381 atatcttttc atagtatagc aatattaaat aaatgcattt ataattttaa ctgtatttta
     4441 aatattaatc atgaggtgat aagatgaata aaatttcaaa gtatattgca atagcatcat
     4501 tatcggtagc ggttacagtt tcagcaccac aaacgacaaa ttctacagcg tttgccaaaa
     4561 gttctgctga agttcaacaa acgcaacaag cttctatacc agcatcacaa aaggcgaatc
     4621 ttggtaatca aaatattatg gcagtggctt ggtatcaaaa ttcagctgaa gcaaaagcat
     4681 tatatttaca aggttataac agtgcaaaga cacagttaga taaagagatt aaaaagaata
     4741 aaggtaaaca taagttagct attgctttgg atttagatga aacagtttta gataattctc
     4801 catatcaagg ctatgcatca atacataata aacctttccc agaaggttgg catgaatggg
     4861 tacaagctgc taaagctaaa cctgtctatg gcgcaaaaga attcttgaaa tatgctgaca
     4921 aaaaaggtgt cgatatctac tatatttctg atagagataa agaaaaagat ttaaaggcaa
     4981 cacaaaagaa cttaaaacaa caaggtatcc ctcaagctaa gaagagtcat attttactaa
     5041 aaggtaaaga tgataagagt aaagaatcac gcagacaaat ggttcaaaag gatcataaac
     5101 ttgtcatgct atttggagat aatttattag actttacaga tccaaaagaa gctacagctg
     5161 aatctcgtga agcattaatt gaaaaacata aagacgattt cggtaagaaa tatatcattt
     5221 tccctaaccc aatgtatggt agttgggaag ctacgattta caacaataac tataaagcaa
     5281 gtgacaaagc aaaagataaa ttacgtaaaa atgctattaa gcaattcgat cctaaaacag
     5341 gcgaagttaa ataatatatg aattggacgt ctacatgtac ttaaaggtat atgtaggcgt
     5401 ttttaaattg atccttaatt ttaaatgtac cgtacaaaaa taatagacaa ttaattgcat
     5461 tttccataaa tttgctttta atataaaaag ttcgggtata atgaaggata agtgtatgta
     5521 taacattaca gagcgcaaca cgacgtacgt gcatctaaga atataaacaa tgtttaaatc
     5581 atagtagtag ggagagaaat gcatgttttt agcttggaat gaaatacggc gcaacaaatt
     5641 gaagtttgga ctaattattg gtgtgttaac gatgattagt tacttgctat ttttattatc
     5701 tggattggcg aatggtctta tcaatatgaa taaagaaggc attgataagt ggcaagcaga
     5761 tgccattgtt ctaaataaag atgccaatca aactgtgcaa caatctgttt ttaacaagaa
     5821 agatattgaa aataaataca agaagcaagc tactttgaag caaacagggg aaattgtgtc
     5881 taatggccat caaaaagaca atgttttagt gttcggtgtt gaaaagtcat catttttagt
     5941 tccgagttta atagaagggc ataaagcgac taaagataat gaagtgttag ctgatgaaac
     6001 gcttaaaaat aaaggattta aaattggtga cacattatca ctatctcaat cagatgaaaa
     6061 attgcatatc gtaggtttta cagaaagtgc aaaatataat gcgtcatcag tcattttcac
     6121 gaatgacgct accattgcca agatcaatcc tagattgact ggagataaaa ttaatgcagt
     6181 tgttgtacgt gatacaaatt ggaaagacaa aaaattaaac caagagcttg aagcggtaag
     6241 tattaatgac tttattgaaa atttaccagg ttataaacca cagaacttaa cattaaactt
     6301 tatgatttca ttcttatttg tcatttcagc tacagttata ggcattttcc tatatgtcat
     6361 gacattacaa aagacgagtt tatttggcat attaaaagct caaggattta cgaatggcta
     6421 tttggcgaat gtggtaattt cgcagacggt catattagca ctatttggta cggcatttgg
     6481 cttactgtta acaggcgtta caggtgcatt tttacctgat gcagtacctg tcaaattcga
     6541 tgtactaaca ttgctcgtat ttgcaattgt gttaatgatt gtctctgtat taggaagttt
     6601 attctccatt ttaacaatta gaaaaataga tccgttaaag gcgattgggt aggaggtgta
     6661 gcaaatgttg aaatttgaaa atgtaacaaa gtcatttaaa gatgggaatc gtaacattga
     6721 agcggttaaa gatacaaatt ttgagataaa taaaggtgat attatagcat tggttggacc
     6781 ttctggctct ggtaaaagta catttctaac tatggcaggt gctttacaaa caccgacatc
     6841 tgggcacatt ttaatcaata accaagatat tacgacaatg aagcaaaaag cattggcaaa
     6901 agttagaatg tctgaaatag gttttatttt acaagctaca aaccttgtac catttttaac
     6961 ggtaaagcaa caatttacat tattgaaaaa gaaaaataag aatgttatgt ctaatgaaga
     7021 ctatcagcaa cttatgtcac aattaggtct aacttcattg cttaataagt taccttcaga
     7081 aatttcaggt ggtcagaaac aacgtgtggc gatagccaaa gcgttatata cgaatccgtc
     7141 gattatttta gcggatgaac ctaccgcggc gttagatact gaaaatgcga ttgaagtcat
     7201 taaaattcta cgtgatcaag ccaaacaaag aaagaaagca tgtattattg ttacacatga
     7261 tgaacgactt aaagcatatt gtgatcgttc atatcatatg aaagatggcg tccttaatct
     7321 tgaaaatgaa acagtagaat agttttatta agccggtaca tcatgtgccg gtatttttat
     7381 gtttatgtat tatttgaata aactttcaca ttcaattaat aataattatt atcgaaaatc
     7441 agaaatattc cgtgaaatat aatatttttt gtagtaaaat ggcctctaag tattcaatat
     7501 ttaaatatgg ggattgaata taaaattatc gtaatggggg tcaatggtta tggatttatt
     7561 gataggtact ttatttttat ttttggtctt agtgattttt acattattta catataaagc
     7621 gcctaatggt atgcgtgcca tgggagcatt agctaatgca gcaatcgcaa catttttagt
     7681 ggaagcattt aataaatatg ttggtggcga agtattcggt attaaatttt tagaagagct
     7741 aggagacgct gcgggaggtc taggtggtgt cgctgccgct ggattaacag cattagctat
     7801 cggtgtgtca ccagtatatg cattagttat agcagccgcg tgcggtggta tggatttatt
     7861 accaggtttc tttgcgggtt atatgattgg atatgtgatg aaatatacag agaaatatgt
     7921 gccggatggt gtcgacttaa ttggatcgat tgtcatctta gcgccattag ctcgtcttat
     7981 tgcagtatta ttaacgccag tagtgaatag tacattgatt cgaattggtg atattatcca
     8041 aagtagtacg aatacgaatc caattatcat gggtatcatt ttaggtggta ttattacggt
     8101 tgtcggcaca gcgccattga gttcaatggc attgacagca ttattaggtt taacgggtgt
     8161 acctatggct attggtgcca tggcagcatt tagttcggca tttatgaatg ggacgctatt
     8221 ccatcgctta aaattaggtg atcgtaagtc tacgattgca gtaagtattg aacctttatc
     8281 acaagcagat attgtatcag ccaatccaat tccaatctat attacaaatt tctttggtgg
     8341 tgcgattgct ggtttaatta ttgctatgtc aggtttaatt aacgatgcga caggtacagc
     8401 tacaccgatt gcaggatttt tagttatgtt tggatttaat catccgacga caattgtgat
     8461 ttatggtgta gtaatggcga ttgtaggtgc gcttgcaggt tatcttggtt caattgtatt
     8521 taaaaaatat ccaattgtta ctaagcaaga catgattaat cgaggtgcag tagacgcata
     8581 gcatcatcat attgaatagt aaaaacaaat aaaacatagt aacgtgattc agtcgatgta
     8641 acagtcgata atgagtcacg tttttttata gaaaaataca agacataaaa atgtcataat
     8701 ttattgtcga caaatatcat actgtataaa catttatcat tttctcaagt accttttaca
     8761 cgatggaatg aacttacttt ttacgaaatt atgcgtattt tataaacaaa tatcattgat
     8821 ataacggtaa atgtaagcgt ttacaacaga aataacagca tgctacgata tttttgtaaa
     8881 ttcactgatt caagtatttt aagtcaatat gaggagggat gttatgagcg attctgagaa
     8941 agaaatttta aaaagaatta aagataatcc gtttatttca caacgtgaac ttgctgaggc
     9001 aattggatta tctagaccca gcgtagcaaa cattatttca ggattaatac aaaaggaata
     9061 tgttatggga aaggcatatg ttttaaatga agattatcct attgtttgta ttggcgcagc
     9121 gaatgtagat cgtaagtttt atgtgcataa aaatttagtt gcagaaacat caaatcctgt
     9181 aacgtcaaca cgctctattg gtggcgtagc aagaaatatt gctgagaact taggtaggct
     9241 tggcgaaacg gtcgcttttt tatctgctag tggacaagat agtgaatggg aaatgattaa
     9301 acgattgtcc acaccattta tgaatttgga tcatgttcaa caatttgaaa atgcgagtac
     9361 aggttcatat acagctttaa ttagtaaaga aggcgacatg acatatggct tagcagatat
     9421 ggaagtgttt gactacatta cgcctgaatt tttaattaag cgttcacact tattgaaaaa
     9481 ggctaagtgc attattgtag atttgaattt aggcaaagag gcattaaact tcttatgtgc
     9541 ctataccacg aaacatcaaa tcaaattagt tatcaccacg gtttcttccc caaaaatgaa
     9601 aaatatgcct gattcattac atgctattga ttggattatc acgaataaag atgaaacaga
     9661 aacatactta aatttaaaaa tagaatctac tgatgattta aaaatagctg ctaaacgctg
     9721 gaatgattta ggtgttaaaa atgttattgt gacaaatggc gtgaaagaac tcatttatcg
     9781 aagtggtgag gaagaaatca ttaagtcagt tatgccatca aatagtgtga aagatgttac
     9841 aggtgcaggc gattcattct gtgctgcagt agtgtatagc tggttaaatg ggatgtctac
     9901 tgaagatata ttaattgctg gtatggttaa cgcaaagaaa acgatagaaa cgaaatatac
     9961 agttaggcaa aacctagatc aacagcaact ttatcacgat atggaggatt ataaaaatgg
    10021 caaatttaca aaagtatatt gagtattctc gagaagttca gcaagcacgg gagaacaatc
    10081 aaccgattgt agcattagaa tcaacaatta tttcgcatgg tatgccgtac ccacaaaatg
    10141 ttgaaatggc aacaacagta gagcaaatta tcaggaataa tggtgccatt ccagcaacca
    10201 tagccattat agatggcaaa attaaaattg gtttagaaag cgaagattta gaaatactgg
    10261 caactagtaa agacgttgct aaagtatcta gaagggattt agcagaagtt attgcgatga
    10321 agtgtgttgg tgctactact gtagcgacga cgatgatatg tgctgcaatg gctggtattc
    10381 aattttttgt tacaggaggt attgggggcg tccataaagg tgcagaacat acgatggaca
    10441 tttcagcaga cttagaagaa ctgtctaaaa caaatgtcac tgttatctgt gcaggtgcca
    10501 aatcaatttt agacttacct aagacgatgg agtatttaga aacaaaaggc gttccagtta
    10561 ttggatatca aacgaatgaa ttgccagcat tcttcactcg cgaaagcggt gttaagttaa
    10621 caagttcggt tgaaacgcca gaacgacttg ctgacattca tttaacaaaa cagcagttaa
    10681 atcttgaagg tggcattgtt gttgctaatc caattccata tgagcatgcc ttatcaaaag
    10741 catatattga ggcaatcata aatgaagctg ttgttgaagc ggaaaatcaa ggtattaaag
    10801 gtaaggacgc cacaccgttc ttgttaggga aaattgtaga aaaaacgaat ggtaaaagtt
    10861 tagcagcaaa tataaaactt gttgaaaaca atgcggcgtt gggtgctaaa attgctgtcg
    10921 ctgttaataa attattgtag gtgatgatac atgaatattt tattcgctat cacagggata
    10981 gcatttgcac tatttgttgc gtttttattc agttttgatc gtaaaaaaat agacttcaaa
    11041 aagacgttaa taatgatatt tattcaagtg ttgatcgtgt tatttatgat gaacacaacg
    11101 attggtttga caattttaac tgcactaggt tcattttttg aagggctaat aaatattagt
    11161 aaagcaggca taaattttgt ttttggagat atacaaaata aaaatggctt tacgttcttt
    11221 ttaaacgtat tactgccatt agtttttatt tctgtattaa taggcatctt taattatatt
    11281 aaggtattac catttattat caaatatgta ggtatcgcta ttaataaaat aactagaatg
    11341 gggcgcttag aaagttattt tgctatttca acagcaatgt ttgggcaacc agaagtatat
    11401 ttaacaataa aagatattat tccaagatta tctagagcga aattatatac aattgcgacg
    11461 tctggtatga gtgctgttag tatggcaatg ctaggttcat atatgcagat gattgaaccc
    11521 aagttcgtag ttacagcagt aatgttaaat atttttagtg cgcttatcat cgccagtgta
    11581 atcaatccct ataaatctga tgatactgat gttgaaattg ataacttaac gaaatccaca
    11641 gaaactaaaa cattgaatgg aaaaacagga aaacctaaga aagttgcctt tttccaaatg
    11701 attggtgata gtgcgatgga tgggtttaaa atcgctgttg tagtagccgt aatgttgtta
    11761 gcatttattt cattaatgga agcaattaat atcatgtttg gtagtgttgg tttgaacttt
    11821 aaacagctta ttggctatgt gtttgcacca atcgcattct taatggggat tccatggagc
    11881 gaagctgttc cagctggctc tttaatggcg actaaattaa ttacaaatga gtttgtagca
    11941 atgcttgatt ttaaaaatgt cctgggtgat gtatcagctc gaacacaagg tatcatttca
    12001 gtttacttag taagcttcgc taattttggt acggttggta tcatcgtagg ttcaattaaa
    12061 ggcattagtg ataaacaagg agaaaaagtt gcatcctttg caatgaggtt gctacttggt
    12121 tcaactctag cttcaatcat ttcaggatca atcattggct tagtattgta aatgaatcga
    12181 agtacctaaa ttaaattcat ggcaaagcta aaccccgtca ccaagttggc gcaacagcgc
    12241 atcataactt agtgacgggg ttttatcata acaatctact ttttcgtagc cgtttttgaa
    12301 atgtatgttg atggtttatc tttttcaaaa attgttaatc ccgttatatc ttttttatgt
    12361 tttgaaggga caatgaagct aagtatataa gcaaagacaa aagcaactgt aaatgaaatg
    12421 gtagatacat agaaaggtga gttacctttg ccaacaccat tatagacata agcaaagatg
    12481 atacccaata ttaatccaca aataacaccg aatgtattcg tacgtttagt gaaaatacca
    12541 actgcaaata caccagccaa tggaacgccg aataatccag tcacaaacaa gaataaatcc
    12601 cataagtcat ttgaattaga agcaattaag tatagtgaca ttccaaaacc gaaaatacct
    12661 gcaatgataa taatgaaacg tgcaaagtta acttcgtgtc gctcgctacc ttttccgaag
    12721 aagcgttgct taatgtcgat tgaaatacaa gcagatatag aatttaaact agatgaaatg
    12781 gtagactgtg cagcggcgaa aatggctgca ataagtaatc ctgctacaaa tggtggcatc
    12841 tcagtcaaaa tgaaatatgg cactacagat gatgtattga agccttttgg taaaacagct
    12901 tcatgtgtat aaaatgaata cagcattgta cccataccat aaaataaggg tgctgaaatt
    12961 aaagctagga taccatttgt ccataacgat ttatttgttt cttttaaact atcagaagct
    13021 tgataacgct gcacgacgtc ttgactcgct gtgtattgat acaagttgtt gaaaatattt
    13081 cctaggaaaa taattggaat ggcagctgcc gcagtattta gtttccaatt gtctgcacta
    13141 attaattttt tgtgctcaat cgcatctgca aagacagtgc cgaaaccgcc tttaatgttc
    13201 acaacaccta gaataataat aactaaagcg ccgcctaata aaatgacgcc ttgaatgaaa
    13261 tcactccaaa ccacaccttc gaaaccacct aaaaatgtat ataaaataca tagtaaacca
    13321 acgagtgatg caacgatata agggttcatg tctgatacag atgtgattgc taatgttggt
    13381 aagtagataa caattgcaac acgccctaaa tggtaaacga caaataataa tgagccaatg
    13441 acacgtatgc tagggccaaa tctagcttct aaatattcat atgcagatgt tacctttaac
    13501 tttttaaaga aagggacata gaaataaata agtaatggaa taattgcgac gatagcaatg
    13561 ttaccagcga tatatgacca atctgttaaa aatgctttct ctggtgtcga cataaatgta
    13621 atcgcactta acgtagtagc ataaattgaa aagccaacta cccaagatgg caagcgacca
    13681 cttgcggtaa agaaactatt ggtactttgg ctcgcgcgct tggtaaaata aacgccaatg
    13741 aacaacatag ctagtagata aatgataacg gcaacccagt ttagtgtgcc aaatccaact
    13801 tctttcatgg gcaacatccc ctttacaatg tattgattct ttgatgtcta taaatcgtat
    13861 tttgcaatga gttgatctaa tgtttgtcga tgtgcttcgt taaaaggttt gaaaggtctt
    13921 ttcggtaatc ctgcatcaat gccacgatga cgtaatattt ctttcaatgt tggataaatc
    13981 cccattgata acactgtttc gataatgtcg tttgaatcat gttgcagttg gtaagcttct
    14041 tgaatttgac cttgtcgtgc taagtcgaag atttttcttg cacggcgacc attaacgtta
    14101 tatgtagaac caattgcacc atctacgcca gaaatcgtag cttgaactaa catttcatca
    14161 aagccagata agattaattt gtctgggaat gcttttctaa tacgttcgag taggaagaag
    14221 tttggcgctg tatatttaac accaacaatt ttttcatgat taaatagctc gctgaattgt
    14281 tcaatagaaa tattcacacc tgttaaatct ggtattgcat aaataatcat attgttctga
    14341 gttgcttcga taatatcgaa atagtaatct ctaatttctt caaaagtaaa tggatagtag
    14401 aatggtgtta cggcagaaag tgcatcataa ccgagttctg tggcatattt tccaagttca
    14461 atggcttcat ttaaatctaa cgaacctact tgagcaatca atttcacttt atccccaact
    14521 gcctctttgg caaccttgaa aacttgcttc ttctgctctg tatttaataa aaagttttcg
    14581 cctgagctac catttacata aagaccgtct aattcttcag tttcaatggc attttgagca
    14641 atttgtttaa gtccttgttc atttacttga ccattttcat caaaaggaac gagtaacgct
    14701 gcatataaac cttttaaatc tttgttcatt atgaagtccc tccaaaaatc atttgataat
    14761 atagtttaca gctataattg taaacgctat cataaaatgt aacaatatct ttttgaaaat
    14821 tgtagtcata tttatgtata attaatgaaa atgtttttca aaatcaatag aaatggagtg
    14881 agtaaggtgt attacatcgc aatcgatatt ggaggcactc aaattaaatc ggcagttatt
    14941 gataagcaat tgaatatgtt tgactatcaa caaatatcaa cgccggacaa caaaagtgag
    15001 cttattactg acaaagtata tgagattgta acaggatata tgaagcaata tcagttgatc
    15061 caacctgtca taggtatttc atcagcaggc gttgttgatg aacaaaaagg cgaaattgta
    15121 tacgcagggc caaccattcc gaattataaa ggtactaatt ttaagcgatt attaaaatca
    15181 ctgtctcctt atgtcaaagt aaaaaatgat gtaaacgctg cattactagg cgaattgaaa
    15241 ttacatcaat atcaagcaga acggatcttt tgtatgacgc ttggtacagg cattgggggt
    15301 gcgtacaaga ataatcaagg tcatattgat aatggtgagc ttcataaggc aaatgaagtt
    15361 gggtatttat tgtatcgtcc aactgaaaat acaacgtttg agcaacgtgc tgcaacgagt
    15421 gcattgaaaa agcgcatgat tgccggagga tttacgagaa gcacacatgt gccagtattg
    15481 tttgaagcag ctgaagaagg tgatgatatt gcaaaacaaa tattgaatga gtgggcagaa
    15541 gatgtagcag aagggattgc ccaaatacag gtcatgtatg atccagggct tatattaatt
    15601 gggggcggta tatctgaaca aggagataat ctcattaaat atatcgagcc gaaagttgca
    15661 cactatttac caaaagacta tgtttatgca ccaatacaaa cgactaagag taaaaatgat
    15721 gcagcattat atggctgttt gcaatgatag ttgaaagaag gagtcattct aaaatagaat
    15781 ttgaaaccgt tacgagagat gagagctgtt gttagttcca cacatcacac tctatctagg
    15841 accaatctaa actatatcaa ccaacagtgt gccacgggca aattaaattg aagaagctga
    15901 gatattaaaa ttttagaaaa tgtaaaaaaa tatttggtat tgaaattaaa aaagcaccta
    15961 gcaactcgtt gggacaatca cgatgattgt ctacagttgc aggtggattt gaatatacta
    16021 ctagttattt gttgtctagg ataatagatt tagtatgttg ataagtttga ctcagattcg
    16081 tattttctaa taaatgataa ctcacgatat cgattaaaaa gagtgtcgca atttgtgtgt
    16141 tgataaattg atggtcggta ttacgcgatt gatccgttgt taaaagtact aaatctgcac
    16201 aatctgtaag tttactacct tcaaaatttg tgatggcaac gacatatgca ccatgagatt
    16261 tggcgacttc cgctgcagaa attaattccg aagtattacc actatttgac atagcaataa
    16321 acatatccga atgagatagt agggatgccg atattttcat taaatgtgaa tcggtagtaa
    16381 cattaccttt tagccccata cgaatcatac gataataaaa ttcagtcgct gataaaccag
    16441 agctacctag tccagcaaag agtatatgtc gacttgattg aagtttgtcg ataaaggttt
    16501 ggataatgtc gttatcaata aattcaccag tttgttgaat gatttgttga tgatatttat
    16561 gaattctttg aataattggg ctattttcaa taactgtctc tgtcatttct tgttgaatat
    16621 taaattttaa atcttggaaa ttctcataat ccagcttatg actaaagcgt gtcatcgttg
    16681 ctggtgatgt accaatcgca tgggctaagg agttaatcgt tgaaaaggca tcgctataac
    16741 cattttgtct tatataattg acgatgcgtt tatcagtttt tgtaaataaa tgttgataac
    16801 gttgaacacg attctcaaat ttcattgtgt caccccttca tcttaatgat tactattata
    16861 tatgaaaaat attttcaaga tagtaaaaag cattgataaa aattatctta atgatatatt
    16921 gtaaatgact ttacgtgaaa aaacgactta tggagtgagg aataatgtta ccacatggat
    16981 taatagtatc ttgtcaggca ctaccagatg aaccattgca ttcatctttt attatgtcga
    17041 aaatggcatt agctgcgtat gaaggtggtg ctgttggtat tcgcgcaaat actaaggaag
    17101 acattttagc aattaaagaa acggtagatt taccagttat tggcattgtg aaacgtgact
    17161 atgatcactc agatgttttc attactgcaa cgtcaaaaga agttgatgaa ctgatagaaa
    17221 gccaatgtga agtcattgca ttggatgcaa cgttacagca acgtccgaaa gaaacgttag
    17281 acgaattagt atcatatatt agaacacatg caccgaacgt tgaaatcatg gctgatatcg
    17341 cgaccgttga agaagctaaa aatgccgcac gacttggctt tgattatatt ggcacgacgt
    17401 tacatggcta tactagttat acgcaaggac aattacttta tcaaaatgac ttccaatttt
    17461 taaaagatgt actacaaagt gttgatgcaa aagttattgc ggaaggtaat gtcattacac
    17521 cggatatgta taaacgtgtg atggacttag gcgttcattg ttcagtcgtt ggtggtgcga
    17581 taacacgacc aaaagaaatt acgaaacgtt ttgttcaaat tatggaagat taaatgataa
    17641 cgataaaaaa acgagatgac catcattaat taaaggcacc taattatctt aggtggctga
    17701 atgaatgtaa tgggttcatc tcgttttgtt tgtttatgat agtgatttta ttttcaactt
    17761 tatccaaaaa taagtaaagc gacggggatg gtgattaata gcgacaacgc cacgcgtaaa
    17821 aaccaaatga tgatgagttt ccagacaggt attttaattt cagttgctag tatacatggc
    17881 actaatgctg agaaaaagat aatggctgat acgcttacta caccgacgac aaatttagta
    17941 ctcattgcag ctttagttac taacaaagat ggtagaaaca tctctacaat agaaatcgct
    18001 gacgcttttg ctagtaaagc ctgatcagca attgggaaaa tataaataaa tggatagaag
    18061 atatagccaa gccaatcaat gaatggtgta tagttcgcta caatcagtcc taaaaaacca
    18121 atcgataata tagaaggtaa aataccaaca gtcatttcta aaccgtcttt caaattgtcc
    18181 caaacgttct tcacgagaga tggtgttaat gcattttgtt tcatcgcctc tgcatatgca
    18241 gttttcagtc tgcttccttc aatagcaact tcttgttctc cttcttgtcc gttataatat
    18301 tctgttgatt cattgctgat tggcggtagc catgcagtaa ttgcagtcac gacaaatgtg
    18361 atgactaaag ttatccaaaa gtataaattc caatgcggca ttaatcctaa agttttagca
    18421 acgataatca taaaagttgc tgaaactgtt gaaaagccag tcgcaataat cgtggcttct
    18481 cgtttgttgt acatcccttg cttatagaca cgattagtaa tcaataatcc taaggaataa
    18541 ctgccgacaa acgaagccac tgcatcgaca gcggattttc ctggtgtttt aaaaataggt
    18601 ctcataatag gctccatata aacaccgaca aattctaata agccatagcc cactaataaa
    18661 gaaagcgcaa ttgcacctac tggaattaag atacttaatg gcatcattaa tttttcaaac
    18721 aaaaacggac catagttagc tttaaatagt attgatggac cgattttaaa tacatacatt
    18781 ataccgatca ttgcacctgc aactttaaat aatgtaatga ccaagtttgt gattgaagtc
    18841 ataaaagtac gtctcactat tggtaacgct gtaccaatta aaatcataat cagtgcaaca
    18901 tagggcataa gtggacctat gattgagcga atggctagat gaacatgatc gacgaaaata
    18961 gtgttgttac cattaatcgt aaaaggaata aagaaacata gtatgcccac taaactatag
    19021 acaaaaaaac gccatgcact tggttgttgt gcattagaat gatattgatt cattaaagca
    19081 acccctttgt ttaaatgaat acacaaaact gtatgatgca tcttcccctt aatgagatga
    19141 atcattattt taatttagaa aaatctgaaa acttactata attgtatagt ttgaattatt
    19201 ttcataccaa t
//
LOCUS       DALAGF010000044        19049 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.044, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000044 DALAGF010000000
VERSION     DALAGF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19049
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.044"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            121..1659
                     /locus_tag="O2692_001446"
     CDS             121..1659
                     /locus_tag="O2692_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCZ4299080.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            1694..3826
                     /locus_tag="O2692_001447"
     CDS             1694..3826
                     /locus_tag="O2692_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCZ4299081.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLDQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDVDGNTSFENEQTSYLTSVFEKHLSDEKSEQLLHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFISDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            3838..4272
                     /locus_tag="O2692_001448"
     CDS             3838..4272
                     /locus_tag="O2692_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCZ4299082.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            4272..5378
                     /locus_tag="O2692_001449"
     CDS             4272..5378
                     /locus_tag="O2692_001449"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCZ4299083.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVEDSSIEAGLRRVLAETSQYITNEFDFTEAEQAAYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(5697..13133)
                     /locus_tag="O2692_001450"
                     /pseudo
     CDS             complement(5697..13133)
                     /locus_tag="O2692_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(13410..14765)
                     /locus_tag="O2692_001451"
     CDS             complement(13410..14765)
                     /locus_tag="O2692_001451"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCZ4299084.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIALDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            complement(14792..15724)
                     /locus_tag="O2692_001452"
     CDS             complement(14792..15724)
                     /locus_tag="O2692_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HCZ4299085.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            complement(15726..16535)
                     /locus_tag="O2692_001453"
     CDS             complement(15726..16535)
                     /locus_tag="O2692_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCZ4299086.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            complement(16724..17632)
                     /gene="rocF"
                     /locus_tag="O2692_001454"
     CDS             complement(16724..17632)
                     /gene="rocF"
                     /locus_tag="O2692_001454"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HCZ4299087.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
     gene            17858..>19049
                     /locus_tag="O2692_001455"
     CDS             17858..>19049
                     /locus_tag="O2692_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1181 family transposase"
                     /protein_id="HCZ4299088.1"
                     /translation="MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPS
                     ACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIV
                     DESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLP
                     EHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVK
                     TVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPDYT
                     IFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRHSYELVQGLR
                     KDLRLCNWPKFINRLNSVSKKSVSKGVWKAVKYYRKHQRMLRNTIYYPAFNNGAIEGI
                     NNKIK"
BASE COUNT         5489 a         3284 c         3135 g         7141 t
ORIGIN      
        1 gacactttat ttaattttgt cacatttatt ttgacaaagt tgatttttgt ttatattgag
       61 taacaagtaa cctctctata cactatatat agtcacatat attaaaaaag aggtgtaaac
      121 atgtcacaaa ctgaagagaa aaaaggaatt ggtcgtcgtg ttcaagcatt tggatcattc
      181 ttaagtagta tgatcatgcc aaacattggc gctttcatcg cttggggttt tatcgcggcc
      241 atttttatag ataatggatg gttacctaac aaagatttag ctactttagc aggaccaatg
      301 attacttatt taatcccatt attgatcgca tttagtggtg gtcgtttaat ttatgattta
      361 cgtggtggta tcatcgcagc aacagcaact atgggggtca tcgttgcatt acctgataca
      421 ccaatgttac ttggtgcaat gattatgggt ccacttgttg gttggttaat gaagaagacc
      481 gaccaattga ttcaacctag aacgccgcaa ggttttgaaa tgttatttaa taacttttct
      541 gctggtattt taggatttat catgacgatt gcaggattta aaattttagc accacttatg
      601 aaatttatta tgcatatttt atcagtggca gttgaagcat tagtacatgc acacctactt
      661 ccacttgtaa gtattttagt agagcctgca aaaattgtat ttttaaacaa tgcgattaac
      721 catggtgtat tcacaccgct tggtgcagat caagctgcaa aagctggtca atcaatttta
      781 tacacaattg aatctaaccc tggaccaggt ttaggtatct tacttgctta tatgattttt
      841 ggaaaaggca ctgcaaaagc aacgtcatat ggtgctggaa ttatccactt cttaggtggt
      901 attcatgaaa tttatttccc atatgtatta atgcgtcctt tattatttat cgctgttatt
      961 ttaggtggaa tgactggtgt tgcaacttat caagcaacag gatttggatt taaaagtccc
     1021 gcgtcaccag gttcatttat agtttactgt ttaaatgcac ctagaggtga attcttgcac
     1081 atgttactcg gtgtcttcct tgctgcactt gtatcattcg ttgtagctgc tttaattatg
     1141 aagttcacta gagaaccaaa gcaggattta gaagctgcga cagctcaaat ggaaaatact
     1201 aaagggaaaa aatcaagcgt tgcttctaag ttagtatctt ctgataaaaa tgttaataca
     1261 gaagaaaatg ctagtggtaa tgttagtgaa acatcttcat cagatgatga tcctgaagcg
     1321 ctattggata attacaacac tgaagatgtt gatgcacaca attacaataa tataaatcat
     1381 gttatttttg cctgcgatgc gggtatgggt tctagtgcaa tgggtgcaag catgttacgt
     1441 aataaattta aaaaggcggg cattaatgat attacagtta caaatactgc gattaatcaa
     1501 ttgccaaaag atgctcaatt agttatcact cagaaaaaac taactgatcg tgctattaaa
     1561 caaacaccaa atgccatcca tatttcagtg gataatttcc ttaattcacc aagatatgaa
     1621 gaacttttaa ataatctaaa aaaagatgat caagcataat aattaaataa attaaaaaat
     1681 ggaggatacc gccatgttat tgagtacacg tgaaaaagaa atgatagccc tattgattaa
     1741 gtaccacggt caatatatca ctatacacga cattgctcag caacttgcgg tgtcctctcg
     1801 tactattcac cgtgaattaa aaggtgttga agcatattta acttcatttt cattaacttt
     1861 agaacgcgca aacaaaaaag ggctacgcat tgctggcaca gattctgatt taaacgattt
     1921 gaagcaatcg attgcacaac atcaaaccat tgacttatct gttgaagagc agaaagtaat
     1981 tattatatac gctttgatac aagccaagga gccagttaaa caatatagtt tagcgcaaga
     2041 aattggcgtt tctgtccaga ctttagcaaa gatgttagat gatttagagc ttgatttaaa
     2101 taagtaccaa ctatctttat ctcgaaagcg tggcgaaggc atttacttgg taggtactga
     2161 atcaaagaaa cgtgaatttt taagtcaatt aatggtgaat aacttaaata gtactagcgt
     2221 ttattcagta attgaaaatc attttgtctt tcattcatta aatcaaatcc acaaagactt
     2281 tgttgactta gagcgcattt ttaatgttga aagactatta atggactacc taagtgcctt
     2341 accctaccaa cttaccgaat caagttattt aactttaact gtccatatcg tgctctccat
     2401 ttcacgtata aaaaatggag agtatgtcgc attaaacgat gatatttatg attctgtaca
     2461 aaacacattt gaacacaaag tagcaagcga acttgctgat aaacttggtc aaatatatga
     2521 cgtcacgttt aatcaggcag aaattgcttt cattactatc catttacgtg gagctaaacg
     2581 aaaaaatctt aatgatacat cattaaataa tcgttgtgaa gaaaacaaaa ttaaagcgtt
     2641 tgttaacaaa gtagaaatga tttccggtat gacatttgca gatttggata ctttagtaga
     2701 tggactgacg ctacacctta atcctgcaat caatcgtttg caagctaata tcgagaccta
     2761 taatccgtta acagacatga ttaagttcaa atatccaaga ctatttgaaa atgtaagatt
     2821 agctttaaat gattgttggc ctgatttgat ttttccagag agtgaaattg cttttatagt
     2881 tttacacttt ggtggctcga ttaaaaacca aggtaatcga tttttaaaca tattagtcgt
     2941 ttgcagcagt ggtatgggaa ctagtcgtct attatcaact cgtctagatc aagtttttag
     3001 tgagattgag cgtattacac aagcatcagt cagcgatttg aagtcactag atttaagtca
     3061 atatgatggc attatttcta ctgtgaattt agacatcgac tccccctatt taacggtaaa
     3121 cccattatta ccagatagtg atatcagtta tgtcgcacag tttttaaata caaagtctac
     3181 gttccaagag acgcatgata aatcatcaaa catgattgat aaggatgatg ttcatgttga
     3241 aacgaaagat gttgatggca acacatcttt tgaaaatgaa caaacttcat acttaacttc
     3301 agttttcgaa aaacatttaa gtgacgaaaa atcagaacaa ttattgcatc atatgcgttc
     3361 gggtttaact ttgcttgatt cagtgaaaat agttagtacc gaagttaaac agtggcaaac
     3421 atatatcgca gattatctat atcaatgcga tgtaataaac gatccaacgt cattcgctga
     3481 actactagag caacgattga ttgacaatcc aggatggata ttaagtccat atcctgttgc
     3541 aataccacac ctaagagaca atatgattaa acaccctatg attctaatca cagttttaga
     3601 agaaccgtta acattgccta gtattcaaaa tgacaatcaa acaattaaat atatgatttc
     3661 catgtttatt tctgacaatg attttatggc atcactggta agtgacttgt ccgaattttt
     3721 aagtttgaaa ttagaatcta ttgatacttt tatggaaaat ccacaggaac ttgaaacatt
     3781 attaagaaac aaatttttag aacgaattaa aaaacaattt atttaggagt gtacaaaatg
     3841 agcgaattat ttagtaatga caatatcttt ttaaatgtaa atgttaacag ccaaaatgaa
     3901 gcaattgaaa aagcaggtaa agccttagtt gatagtggtg ctgtaacaga tgcttatatt
     3961 caagcaatga aagatcgtga gcaagtcgta tcaacattta tgggaaatgg cttagcaatt
     4021 cctcatggca cagatgaagc taaaacaaat gtgattcact caggtttaac attattacaa
     4081 atccctgaag gcgttgactg ggatggcgaa gtagttaaag ttgtcgtggg aattgctggt
     4141 aaagatggcg aacatttaga cttgttatct aaaattgcaa ttacatttag cgaagaagaa
     4201 aatgtggatc gtatcgttca agcaaaatct gcagaagaaa ttaaacaagt attcgaggag
     4261 gcagatgcat aatgaaagca gttcactttg gtgctggtaa cataggtcgt ggtttcattg
     4321 gttatattct tgcagacaac aatgttaaag taacatttgc agacgtcaat gaagaaatca
     4381 ttaatgcttt agctcatgat catcaatacg atgttatttt agctgatgag tctaaaacaa
     4441 cgacgcgcgt gaataatgtt gatgcaatta attcaatgca accttctgaa gcgttgaaac
     4501 aagcaattct agaagctgat attattacaa cagctgttgg tgttaacata ctacctatta
     4561 ttgctaaatc ttttgcgcct ttcttaaaag aaaaaacaaa ccatgttaat attgttgctt
     4621 gtgagaatgc tattatggca actgatacat tgaaaaaagc agtacttgat attactggcc
     4681 ctcttggtaa caatattcat tttgctaact cagcagttga tagaattgta ccattacaaa
     4741 agaatgaaaa tatattagac gttatggttg agccatttta cgaatgggtt gttgaaaaag
     4801 atgcatggta tggtccagaa ctaaaccata ttaaatatgt tgatgattta acaccatata
     4861 ttgagcgtaa attattaact gtgaatacag gacatgcata tttagcgtat gctggtaaat
     4921 ttgcaggtaa agctacagtt ttagatgcag ttgaagatag ttcaattgaa gctggcttac
     4981 gccgtgtttt agctgaaact agtcaatata ttactaatga atttgatttt actgaagcgg
     5041 aacaagctgc ttatgttgaa aaaataatag atcgtttcaa caattcttat ttatctgatg
     5101 aagtaacacg tgtcggacga ggtacattac gtaaaattgg ccctaaagat agaattataa
     5161 aaccattaac atatctttat aataaagatt tagaacgcac tggtttatta aatacagctg
     5221 cattgttatt gaagtatgat gatacagcag accaagaaac tgttgagaaa aataattaca
     5281 ttaaagaaca cggtttaaaa gcgtttttaa gtgaatatgc taaagttgac gatggcttag
     5341 ccgatgaaat aattgaagcg tacaattcac tttcataatt tattgagctt tgtttgaaac
     5401 aagaagtttc caacgttatt cgttaacaat cagtaataat gtagtagttc ccttgaatta
     5461 acaatattaa atttctgaac ataaaaaata ctcccttcaa catagacact taacttgtgt
     5521 tatgtatgaa aggagtattt ttgcgttaat aatttgtttt attttcgagc cacagccacc
     5581 tattcaatgg ctattggtca ttactaaaac aaattcatat taactgttag acttggttac
     5641 ttagtaagga atatttccct atgaaataac tagatgttca cattcttgaa taaattttat
     5701 tcttcagttt gttggtcttt cttagtgaat cttctaatta agaatgccat acctgcacct
     5761 agagctaatt cagcatatgg taaatcgtca ttatgtgaca taccagtatc tggtaaagtt
     5821 ttagcttgtt gtttagcttt attaactttt ccttgttgag ctgattttgt cttagcttgg
     5881 tggtcgtcag tgttagttac attaagcata tcttgattag cactattgct tccatttgaa
     5941 actgtagctg gagatgcatt ggcaccgtcg ttttgcgtag ctttattgtt tgcagctgaa
     6001 ccaactgatt tttgcgtatc attagtatct gctgttgccg tatcatcttt ttggctaaca
     6061 ttagttgaag tcattttttc ttttgcttca gaagatgcag atgttgatgg tttattcgaa
     6121 acttcagtat cagctttgct tggcgattta tctgcttcgt tagatgcaac gttagtttca
     6181 gacttaagtt gtcctgcatc agtttgattt gtcgtacttt cttctttatc ttttgatgta
     6241 ttagaaggta catttggttc tgttatgtct gctgaaggca atgtttcagt tgttgattca
     6301 accatacttt gatttgttga atcactacca tctttttctg ccttagcttt attttcagat
     6361 tttggttgtg caaccttgtc attagttgat tgagattcag cactattatt tacttcagca
     6421 ttttgttttg aatcatttac agatgcatta tctttgctat cagcagatga tgctgcttct
     6481 gtgctcgcag ttgttggagc cgttgctgtt gatcctgttg gtgcattctc gtttgttgct
     6541 gtagttgtac tattgttatt tgttgtgctt tctgctggcg ttgcattatc agtttctgtt
     6601 acaggtttat cagttgtgcc gttattagtt gattctactt ctggtttact agttacatcg
     6661 ttatccattg tcggactgtt tgttgatgca tctacactag aattgttatt agcttgcggt
     6721 ttatcatttg catcatcagt tgctgatgtt gctgttgttt cacctgttgc cgcatcacta
     6781 ttatttggtg ttgtcggaga agcgtctgct ttgccattag ctgtcgtctc agatacgtta
     6841 ggttgtccag tattttctgg tgttgcatta gcatttgaat ttgctgttgc atcattatta
     6901 tctataccat tattagtatc attagcatct ggatcattct gaggcacaat cgcttcaatt
     6961 gcaggtatcg ttacattttg taattcagca acttctgcat ttgtttgtgt tttatctaat
     7021 ttatcagcaa atctgtcaaa atatctacct aaatccgtac gtgcaatttc tttcgccgat
     7081 gcatctgcat ctgcattttt aattatttct atttgcttgt taaccacttc tctgattgct
     7141 tccaaagcat ttttcttaac ttcaggatta atacgttgtg ctttaagttg ttcaagcgca
     7201 ctatttttga cagtagcgat ttctgcattt gtagtttgat cagaaatatc ttcagttgct
     7261 tttgataaaa tgtcttctaa agcattcgta aacgcttctt tttcttcagt tgtagcatca
     7321 gcgttgacat ttacacctgc ttcaatctgg tctagtgcag tttctaattc ttcgatagct
     7381 ttttgttttt ctgttgagtc gatttgaatg ttatcaaatg cttcaagtcc ttgagctttc
     7441 gctttttcaa cttcagcagt tgttgttgca tcagtaatac cttgtttagc ttgatctgta
     7501 atttgtttaa tcattgctaa tgcttcagtt ttttcttcag cagttaactg gtcactttga
     7561 tcaatagatt ctttcgtatc ttctgcttta acttcaatag cttggttcgc tttaggttta
     7621 acagtagcat ctacttgaat agcatcaatt gctgctttac cttgtgttaa tgcatcatca
     7681 acgtcaccat tatccacacc attattaatg ctttctaatg cagtttgaac attttggtca
     7741 acttgcttaa ttgcttgttg cttttcatct tgtgttgcat tagtgtcagc tgaaatatta
     7801 tttttcttct gatctgcata agcatataaa tctgttgtag ctgattcttt tttacctgtt
     7861 ggaattgtgt aatcgttaat attatctaag tcattatgaa tttgaacttc aatgtcatct
     7921 ttagaagtag cttgattaac attttgatcc gcagtttgtt ttaattcagc aagtttttgt
     7981 ttcgcttcag caatttcact tgaagtcgat gcgttagagt tatccgcttc gtttacttta
     8041 gcattatatg catcttcaat tttagctaaa gcatcttttt tgtactcact aaatgtttta
     8101 actgcattaa ttttagcttt tccttcttta actgcattat caacatattc atttgttgat
     8161 gattgatcga catttgtttt cgcttgattt aattcagtat caacttcttg ttttgcatca
     8221 ttaatttctt gttgtgatgc atttggtgtt tgttctattt gtgttttctt atcagctgca
     8281 gcttgatcta attctttttt acctgctggt ttcttaacag gatttgcatg aagttgttga
     8341 actttttgta ctgctgtatc tttagcagta gttacatcac ctgtagtagt tgctgcatta
     8401 atattattta aaccttcttc atatgctgct ctaactggtc caatatcgtt acctttttct
     8461 tcattagtag tctcattatt attaagtatt tcagttattt tattttgcat ttcagttagc
     8521 aattccgctc ttgcattcgt tttaacatct gttgatgctt gaattgggtc aattgcttga
     8581 attgcattat cttttgcagt gtttacatca tcgattgact gtgcattttc aatattttga
     8641 ttaccttgtg ttaattgtgc gtctacttgt tgatttgctt gttctttttc ttcagtagtc
     8701 gcatctgcag tttgtgcaat aagcgctttt tgttcgtttg cttttgttgc taattcatct
     8761 ttcgcaacat ctttaattgt tgtatctgca gtaatacctt gaatatcagc aactgcttga
     8821 tctttaattt gcgtaacatc attagttgtt tgtgcattta agatatcttg atacgctttt
     8881 tctttagctt ttaaaactaa atcttttgct gcatttttct cttcagttgt agcaccagtt
     8941 gtattatcaa ttgcttgatt ttgagttgtc acagcttgat caacatcatt tttagcattt
     9001 gatttaaccg ctgttgctgg ttgcgtgctt tgaattgaat tctttccagc gtctttcgcc
     9061 tgatctacac cattatcatc agttgcagct gtaatattat ttttcgcgtc tgtaactgct
     9121 gttgctaatt gttgaatagc tgcttctttt tcttctgttg tagcgttctg atcattattg
     9181 ataacatttg tttgcgttgc ttgtaattga tcaatttcat ctttagccgc ttgtttcttc
     9241 acaacttttg gtgttaccgc attaatcgct gcttctgcat ttgctttagc ttcatcaact
     9301 tgtgcgttag tagttgctgc tgaaatggct tgatttgctt taccattttc agtatttgct
     9361 tcagcatcag ctgcttgttt ttcttcatct gttgcatctg gcgtagcttg aatctcttgc
     9421 aatttgttat ttaaaattgc tgtgatttca ttacgtgcag ttgctttttt attaactgtt
     9481 ggtgttactt gatcaatact attttcacct gtcgttttcg cttggtctac atcattttga
     9541 ctattagcag cttcaatgtt gctatttgct tgtgtcacag cattatctac gtccgcatta
     9601 gccgctgcaa tttcttcagc agtaatgtct tgcgtttgag cgattgctgt tttacgttca
     9661 ttcgctttcg tagcaattgc ttctttcgca ttatctttag ttgttgttgc tggctgaatc
     9721 gcttcaattt tagcaattgc tgctttttta gccgcttcaa cttccgcatt tgtatgtgct
     9781 gcatctattg cggcatcagc tgttgttttt tcagtttgaa cttgttattt agcagctgct
     9841 ttttcttcag ttgttgagcc gttatttcca tcaattgctg tttcttgagc ttgtacttta
     9901 tctgcaattg cttgttttgc tgctggttta acatttgcat caggtgtaat ggctgcgatt
     9961 gtagcttcat ttgtagtttt tgcattatcc acatcattgt ttgctgcagc attatctata
    10021 tcagcgtttg cagtaactac tgcttgatcc actttgtctt tcgctgcttg ttgttcttca
    10081 gtagtcgaat cattcattgc ttcaattgct gttttacgtt cacttgcttt ttgagcgatt
    10141 tccgcttttg catccgattt cttagttgtg gcagcttgaa cttgattaat tgcagcaata
    10201 ctattgtctt tagctgttgt tacatcactg tttgtatttg cagcatcaag atttgttctt
    10261 gcttcttgct ttttagtatc taattctgta tatgcagctt gtttttcttc tgtagttgaa
    10321 gcattgctat tttgaatttc ttgtttacgt gtattatatg cgttttctac ttccgtatca
    10381 gctgcaggtt taacttttgc ttgtgtttga attgcattga ttttatctaa tacttttgat
    10441 tttgtatcag caacttcctg ttttgattta acaacttgga tgtcatgtaa accttgcttt
    10501 tgagctgcat ctactgctgc atcagcttct gctacttctt catttgttgc atttgaaact
    10561 gtagcatttt gagcttttct agctgttgca gcttgtttaa cttcattata ggcatccatt
    10621 tttgtttgcg tagagtcagt aatattttca atttttgaga tttcttcgtt tttaactctt
    10681 tctaaatctt gtgcagtcgt tgtcgcttca attgctttaa caccagaaac tttagctgca
    10741 ttaatacgtt caatagcttc cgctacttct tcatttgttg tatcaggatt taaaggtgct
    10801 tgatcaattt gtgcttgaac aacttcgtca aattcttcaa gagctgctgc tttagctgtt
    10861 gtagcaggtg tagtttgatt aatatcattg attgcttttg ttttaatggc ttctacttgt
    10921 gcatttgttg ttgctgcatc aatatcttta attgcctttg tttcaatttt accaatttta
    10981 tcatttgcta catctttttc atcttgtaat gatgcatttg actttttaat ttgttgttta
    11041 cgagttgtaa ctgcttggat aatatcttgt ttcgcttgag gtttaactgt tggtgtaatc
    11101 acatcttgtt ctaacactgc gataccatta tctttttcag ttgtgacacc ttgtgctgta
    11161 gtttgtttat taatttcagc gattgcatta tttttatcat tgttaatttt agttacaagt
    11221 gctgcaactt catcttgcgt aactttttta ctttgttgtg ctgctgtaac cttttcttga
    11281 gctttagtat ctaattcagc aattgctgct tgattatcaa ttaatgcagc ttgtaattta
    11341 gttactaaac catcaatatc cgcttgagat gcacgatttg cagttttaac atgattcgca
    11401 tcttcattta aaatagtatc tgcttgttgt ttaagtttat tgtattctgc aattgatgct
    11461 gttgtgtaat gactattatc aacttgtgag tttacttgtt gttgcaacgc atctttgttc
    11521 atttcaaccg ctactgaaaa tggatctgca gttagtgtaa cttctggttg cgcattatta
    11581 attacaacat ctgaagcagt acgatatgtt aatttttcat taaaatcaat atttttaggt
    11641 gtatcgatat tcgcaacatt aactttatat gataatttta aagatttatc tgggaataaa
    11701 acttctttag tgtgtgtacc acgtgccgtt gtcacacctt ggcttgtaaa tgtaacttta
    11761 tttgcatttt gatcataatt aacagtcata tttttcaata ctgtactgtc ttcattacca
    11821 ttagggaatg ttgtagttaa tgaattatta acgtaagtta caccttgtgg taatgttact
    11881 tcatatttaa attgatctgc ttttaatgaa gcaccaaaat taccattgtt accgatttca
    11941 gtattaatag tatattcttt attgttagtt gctttcggtg ctaaatctct atttttgaca
    12001 tataaatgtg atcctgagaa tagacctaca ttatcaatga atgaatagta tttataacca
    12061 tctttatttg ttgtaattct tgaagcatca gcaactgctg tattgtcagg aataaattgt
    12121 aaatctaaac gatcagcatc agttggtact ttaaataaat gtgcagtacc agtcttagca
    12181 acatttacag ttgctaattc agtatttttc ttagcatctt taataactaa atttgttgca
    12241 ccttgcttac tattcgtttg catagtagta aagttaataa ttaattctga atctggtttt
    12301 acatttacag tttttgaaat accgttaaag ttaccatgat ctgtagaatc atttgcattc
    12361 acacgaccta atgcagccac gtttccttta gcttgatagt tttgagggtt attcttatca
    12421 aacatatcgc ttcgtcttaa ttctgagtta acgaaaccaa tcttaccgtt gttaattaat
    12481 gaataaccat ttactttatc tgtaacagtt acagttggat cttgtctatt ctcatctgtt
    12541 gatatggcag gatcatcaaa tgttaatgtc gtattaatac tgccttcacc agtattgcta
    12601 gcatttggat cttgagtttg tgcgtttgct gctacaggtg ctgctggttg cgctgctgct
    12661 ggagcattcg ctggctgtgt ttgatttgcc ggtgttgcat tattattagg tgttgcttgg
    12721 ttatttcctt gacctgcttg gtttgccggt gttgcttgat ttccaggttg tgcttgtgca
    12781 gcgttattcg gatcagcttg attaccttgt ccagctggtt gtgtatttgg ttgtgctgct
    12841 cctcctgctg gattagcctg tccaccttgg tttgctggtt gtactgctgg ttgtccttgg
    12901 ttggcaggtg cagctggctg tgctgtagga ttagcttgag caccagcatt tgcgttaggc
    12961 tgtgtattgg catcagctgg ttgtgctggt tgattttgtg caggctgatt ttgctctgct
    13021 gcagacgctg ttgtcgggtt agtagatata aaagtaacag tggcaattaa agctgaaaaa
    13081 ataccgacat taaattttct gatactaaat ttttgttgtc tgaataaatt cattaagtca
    13141 tcctcctggt tgattattct cgctgttaaa tgatttcact taatcaactg ttaagataag
    13201 tagtagcatc tgcgttaaaa acacaaagca actctatcta attaaaatta attttatcat
    13261 cattatatat tgagtaccag tgtattttat attacatatt gattactttg tttttatttt
    13321 gtttatatca ttttacgttt gtactataaa ttatttctac aaacacaaaa aaccgatgca
    13381 tacgcatcgg ctcatttgta atacagtatt tatttatcta atcccatttt atcttgaacc
    13441 acatcagcta tttgttgtgc aaatctttca gcatcttcat cagttgctgc ttcaaccatg
    13501 acacgaacta atggttctgt tccagaaggt cttactaaaa ttcgaccttc tccattcatt
    13561 tctacttcta ctttagtcat aacttcttta acgtcaacat tttcttcaac acgatattta
    13621 tctgttacgc gtacgttaat taatgattgt ggatattttt tcatttgtcc agctaattca
    13681 cttagtgatt taccagtcat ttttattaca gaagctaatt gaataccagt taataaacca
    13741 tcaccagttg tattgtaatc catcataacg atatgtccag attgttctcc acctaagtta
    13801 taattaccgc gacgcatttc ttctactaca tatctgtcgc caactttagt tttattagat
    13861 ttaattcctt cttgttcaag cgctttgtaa aaacctaaat tactcataac agtagaaaca
    13921 atcatgtcat tattcaattc ttgattttta tgcatttctt gaccaataat aaacataatt
    13981 tggtcaccgt caacgatttg accattctca tctactgcta tgattctgtc tccatcgccg
    14041 tcaaatgcta acccaaaatc actttcagtt tcaactactt tttcagctaa tttttcagga
    14101 tgtgtagagc cacatttctc attgatatta tatccatcag gactacatcc aattgtttca
    14161 gtatctgctt ctaagtcacc aaataagaat ggcgctagtg atgatgttga accatttgca
    14221 ccatctaaag caattttcaa accttcaaag ttaacatcta ctgttgattt taaatagctc
    14281 aaatattttt gtgccccttc aaagtaatct gaataatgta caatatcatt gccaactggt
    14341 cttggtaatt ctgggttttc ttgatccaat aatgcttcaa tttcattttc ttgttcatct
    14401 gatagtttaa aaccatctga tccaaagaat ttaataccat tatctgcaac tggattatga
    14461 gaggctgaaa tcattacacc taactctgca cccatatcgc gtgttaaata tgcaacacct
    14521 ggtgttgaaa taatacctaa tcgcatcact tctgcaccaa ttgaaatcaa accagctatt
    14581 aatgctgatt ctaacatttc acctgaaact ctagtatcgc gacctacaag tacacgtggg
    14641 tgtttttcac ctttattatg tgctagaaca tagccaccgt atcttcctaa tttaaatgcc
    14701 aattcaggtg ttagttcttg gtttgcgaca cctcttactc cgtctgtacc aaaatatttt
    14761 cccattgttt actctccttt aatttagcta tttattttac atttatataa gccttcgttt
    14821 cacttggttg tgctttagtg acatgttctg gtaaattgat ttttacagtc ttttcagttg
    14881 attctgaaat accatctaaa tctacttctg catcaacttc gcttatattt tgtaagtcat
    14941 ctcgactacc gaagatttca atttctttat cttctaaatc aatcgaactt aactctttat
    15001 catctgctaa actacctttc tgtttaacat ttactttaac ctttttgcta aaaggctcta
    15061 ctttaacttg taaattcact tcattaggtt gaatcgatac atttaattta ttcagttttt
    15121 tatcaaaagc cgttacttct gcgacatctt ttgtgtcacc attaatcttt ttattagttt
    15181 taaaagtggc ttttaaataa gcgatatcat tcaattgttc ttctccacct gttactttaa
    15241 ctgtttgtgg tgaaacttct tgctttgtaa ttttataaag tggatcaata tcactctgac
    15301 ttacatcagg ttgaactgtc atctttttag ttactttgtt ttcaagcgta atatttgcta
    15361 atttaggttt tacagaataa tgaatgtcat cagctaaccc tttaacttga tacttctctt
    15421 gatattttcc agctttagca tttgataaat caatcactac tcttaaatct tctggatttt
    15481 caatttttat tatctttgat tgtggtcctg aaatagtcac attaactgtt tcaggcgctt
    15541 ttgttaaatg caaatcttta gtgttataaa gaatttcaac gggtacatct tgaatcgttt
    15601 tactagactt ttgaccaaga ttaccagtgt taaagatatt tccaaaaaca ttgttaacag
    15661 ataaaaagaa aaacaatgcc aaaagaaagg caataaatct caagccccat ttactttcta
    15721 gcatattatt tcacaccttt cttttgaaag cgtgtgccaa accaatgttc agcaagcaac
    15781 tcttcaaaaa tttcgtttga aatgtctcgt cgtaattttc catcaaatgt taccgaaata
    15841 tcaccagttt cttcagatac aataacggta aatgcatcag atacttctga aataccaacc
    15901 gcagctctat gtcttgtacc caaactttta gatatcttag gactatcaga caatggcaaa
    15961 taacttgctg ctgctgcaat cttcgtgcct tgaataatca ttgcaccatc atgtaaaggt
    16021 gtgttaggta taaagacatt aattaaaagt tcttgcgaaa tatttgaatc cattgcaata
    16081 cctgtttcaa tataatcttg aagacctgtt tctttttcaa agacaattaa tgcacctata
    16141 cgtcttttag ccatatattg cacagccttt gaaaccgatt gaatcaattt ctcttcatct
    16201 ttactatacg tattagaagt atagcgtttt aaaaagctac ctctaccaag ttgttctaac
    16261 gcacgtctaa tttctggttg gaatattact attaaagcta atacccccca ttgaataacg
    16321 atatcgaata atttagatgt tgcagtcaag ttcaatatca tacttatctg ctgaccaata
    16381 acaattacta atatcccttt aagtaattgt atcgctttag ttcccttaaa gaccgtgatg
    16441 agaagataaa gtacatacca aactatcagt aaatcaagga tactcgttac aatttttaac
    16501 gtactgaggt tttgaaaaaa gttggaaaaa tccataacat ctcctccggg taatattttt
    16561 ccataatacc cattatacca atcatttaca tcactgtcat acgaacatat atttaaatag
    16621 aaaaaattta ttttaaagat tataactact cttaatcatt ttagtgaatt aaaaaaagta
    16681 gtgcaaaaag caaaatatac tttatacact acaaatcatt tatttataat aaagtttcac
    16741 caaaaaatgt tccaactaat gaaaccgctt gttcagcagt atgattatta ctgtcaatca
    16801 atggatttac ttcaactaaa tccattgagg aaattaaatg tgattgatgc agtaattcca
    16861 atgcaaaatg gctttctcta taactaagac cacccaaaac tctagtacca gtgcctggcg
    16921 tttcaagcgg atctaaagca tcaacatcta aagataaatg aacgccatca acattgcgtg
    16981 acttcaaata ttctattgta ttttcaatta cttcctttat ccccaattta tcaatatctg
    17041 acatagtaaa tgttttaata ttatgatctt tgataaattg tctttcacct ttatctaaat
    17101 ctctcatacc aattagtacg atgttttctg gcttgattac attactattt aattctaaaa
    17161 gttctttggg accttcgcct gtcaaaatcc ttagaggcat accatgaata tttccacttg
    17221 gtgactcttc aggtatattt aaatcaccat gtgcatcata ccaaataaca cctaaattat
    17281 tataatgttt acttattgct gatactgaac ctaccgcaat agaatgatct ccaccaagaa
    17341 ctagaggaaa tctgttattt tcaattgatg ctgaaacctc tttatttaat ttttgattaa
    17401 catctataat ttcatcataa tttcttaatc ctttttgttc actatgaaat ttttcaatgt
    17461 tcacagcagg taccttaata tcccccttgt catatacatc aaggtctaat tgctttaatc
    17521 ttgaaattaa tccagcatat ctaattgctg ttggtcctaa atcaacacct aattttcttt
    17581 gtccaaatgt tgatggtgca cctataatat caattgcttt tgtctttgtc ataagcgtcc
    17641 ccctttgctc tatgtaatta aagaataatg tatgcgctta ccattatcaa gcaatagcta
    17701 cacatataat ctgtttatct tattacttca taaaaaaagg ttcttcatct tttatggtgg
    17761 gaaggtaaaa cttcctgctt tttttaatac acaaaaagcg caattgcctc tataatttaa
    17821 agtgaccaaa cccaaactaa aggagacaag tgcgcctatg tgtaatgata ccttagaatt
    17881 actaagaata aaagatgaaa atataaaata tataaaccaa gaaattgacg tcattatcaa
    17941 aggaaaaaaa gcaacagtgg ttaatgctgt actaacgtat aagccttcgg cctgttattg
    18001 ttgtggagtt aaaaatgaag gacaaattca taaacatggt aagcgtgttt ctcgtattac
    18061 tttacttaaa actcaagggt ataacacata cctcaactta gctaaacaac gttttaaatg
    18121 cttagaatgc aatggcactt ttactgctaa aacgtcaatt gttgatgagt cgtgttttat
    18181 ctcaagatgt gttactcaaa aagttataga agaagctact aaagttaaaa cagagattga
    18241 tactgcagaa gataactgta tctctccatc tactgtaagt cgtattagaa ctaaagcggc
    18301 taattcatta cgaattaaac cctttaattg tttgccagaa cacatcgcta tggatgaatt
    18361 taaaagcgtt aaaaatgtaa ctggatcaat gagtttcatt tttatagata atgatactca
    18421 tgatgttata gatattttag aaaatagaac tacaagattc ttgcgtgcct atttcgagcg
    18481 attcgattta aaaaatcgac aacaagttaa gacggttact attgacatgt atgaacccta
    18541 tgtccgatta tttcgcgacc tatttcctaa tgcagctatt atttttgaca gattccatat
    18601 cgttcaacat ttaaatagag aacttaataa gtatcgtgta caagttatga atgaataccg
    18661 taataaaaaa ggacctgatt atacaatttt taagaataac tggaaagtcc tattgatgga
    18721 tactagtaaa accatattta gtaaatacag atggaataaa tcttttaagg cttataaacg
    18781 ctcatctgac attgtagaat tcatgctttc aaaagacgat atactacgac actcctacga
    18841 acttgtccaa ggattacgaa aagacctaag gttatgtaat tggcctaaat ttattaatcg
    18901 tttaaattca gttagtaaaa agtctgtgag taagggtgta tggaaagcgg ttaaatatta
    18961 tagaaaacat caaaggatgt taagaaatac aatttattac ccagcattta ataatggtgc
    19021 tatagaagga attaataata agataaaat
//
LOCUS       DALAGF010000045        18891 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.045, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000045 DALAGF010000000
VERSION     DALAGF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18891
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.045"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            631..2040
                     /gene="sapep"
                     /locus_tag="O2692_001456"
     CDS             631..2040
                     /gene="sapep"
                     /locus_tag="O2692_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HCZ4299089.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            2044..2892
                     /gene="dat"
                     /locus_tag="O2692_001457"
     CDS             2044..2892
                     /gene="dat"
                     /locus_tag="O2692_001457"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HCZ4299090.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
     gene            3442..4233
                     /locus_tag="O2692_001458"
     CDS             3442..4233
                     /locus_tag="O2692_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCZ4299091.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
     gene            4248..4892
                     /gene="trmB"
                     /locus_tag="O2692_001459"
     CDS             4248..4892
                     /gene="trmB"
                     /locus_tag="O2692_001459"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCZ4299092.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
     gene            5362..6204
                     /locus_tag="O2692_001460"
     CDS             5362..6204
                     /locus_tag="O2692_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCZ4299093.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVLGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDNGENIDAYILRETL
                     VDNN"
     gene            complement(6329..6640)
                     /locus_tag="O2692_001461"
     CDS             complement(6329..6640)
                     /locus_tag="O2692_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCZ4299094.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            6727..7803
                     /locus_tag="O2692_001462"
     CDS             6727..7803
                     /locus_tag="O2692_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCZ4299095.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            7868..8179
                     /locus_tag="O2692_001463"
     CDS             7868..8179
                     /locus_tag="O2692_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCZ4299096.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            8279..9136
                     /locus_tag="O2692_001464"
     CDS             8279..9136
                     /locus_tag="O2692_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HCZ4299097.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            9165..9761
                     /locus_tag="O2692_001465"
     CDS             9165..9761
                     /locus_tag="O2692_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCZ4299098.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            9782..13606
                     /locus_tag="O2692_001466"
     CDS             9782..13606
                     /locus_tag="O2692_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCZ4299099.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEKRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKPKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNIEDNTNRNASEMHVDAPKTQEY
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDNHVVEDSSMNEIEK
                     NNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSERMPASQATPSSRSDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSVDSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQSSFSNKNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            13630..14943
                     /locus_tag="O2692_001467"
     CDS             13630..14943
                     /locus_tag="O2692_001467"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCZ4299100.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVILFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            15017..15508
                     /locus_tag="O2692_001468"
     CDS             15017..15508
                     /locus_tag="O2692_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCZ4299101.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            15582..16781
                     /locus_tag="O2692_001469"
     CDS             15582..16781
                     /locus_tag="O2692_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299102.1"
                     /translation="MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQAEADKSVAVSNKESKAVALKAQQAAI
                     KEEASANNLSDISQEAQEVQEAKKEAQAEKDSDTLTKDASAAKVEVSKPESQAERLAN
                     AAKQKQAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDNKD
                     TVKQKEAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQ
                     KKQHNKKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKAKSKI
                     EKRTFND"
     gene            16916..17020
                     /locus_tag="O2692_001470"
     CDS             16916..17020
                     /locus_tag="O2692_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299103.1"
                     /translation="MRSFHVFMLFETSLDNNSDIAHFKLKMEKYSIIK"
     gene            17481..18572
                     /locus_tag="O2692_001471"
     CDS             17481..18572
                     /locus_tag="O2692_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HCZ4299104.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
BASE COUNT         7140 a         2557 c         3507 g         5687 t
ORIGIN      
        1 attttcattc agtcaactac tgccaatata atattgtaaa ctataggaca tttattagtg
       61 tttcaggttc tttatcgtta gtcatagcta atatgcatat agaaagaaga atacatgcgt
      121 gaaactcatt cataagaaat actaatatct aaagaaaacg tatttcttta tcttttcgaa
      181 attctctgtg ttggggccct taccccaact tgcacattat tgaaagctga ctattggcca
      241 gcttctgtgt tggggccctt accccaactt gcacattatt gaaagctaac tattggccag
      301 cttctatgtt ggggccccgc caacttgcat tgtctgtaga atttcttttc gaaattctct
      361 atgttggggc cccggggcgc attttcgttc ggttaattac tgtcaatatt aaattgtgtg
      421 cctagtacat tgacttatat ctcgagctct tgaatatgtg tattttaaat atataaatta
      481 aaaaaggaac aaagcagcta tttatgattt gtgtcatatc aatagcatcg ttttgttcct
      541 ttttattatt ttctgacatc ttattgtagt ttttatatct tttcatgtaa aatagaattt
      601 aacttataga aaaaaggaag tcgatttcga atgtggaaag aaaaagttca acaatacgaa
      661 gatcaaatca ttaatgactt aaaaggatta ttagcaattg aaagtgtgag agatgatgca
      721 aaagcatcag aagacgcacc agttggtcca ggtcctcgta aggcattaga ctacatgtat
      781 gaaattgcac atagagatgg atttacaaca catgatgtgg atcatattgc aggaagaatt
      841 gaggcaggta aaggaaatga cgtattaggt atcttatgtc atgttgacgt tgttcctgct
      901 ggtgatggat gggatagtaa tccgttcgag ccggttgtaa cagaagatgc tatcatagct
      961 agaggtacac ttgatgacaa aggtccaaca attgctgctt attatgcaat taagatatta
     1021 gaagatatga atgtggattg gaagaaacgt attcatatga ttattggtac ggatgaagaa
     1081 tctgattgga aatgtacgga tcgctatttt aaaacagaag aaatgccaac attaggtttt
     1141 gcaccagatg cagaatttcc atgtattcat ggtgaaaaag gcattacaac atttgattta
     1201 gttcaaaata aacttactga agatcaagat gaacctgatt atgaattaat aacttttaaa
     1261 tctggtgaac gttacaacat ggtacctgat catgcagaag caagagtgct tgttaaagaa
     1321 aatatgacag atgttattca agactttgag tactttttag aacaaaatca tttacaaggt
     1381 gatagtactg ttgatagtgg cattctagtt ttaacagttg aaggtaaagc ggttcatggt
     1441 atggatccat ctatcggtgt gaatgcgggt ctttacttac taaaattctt agcatcatta
     1501 aatcttgata ataatgcaca agcgtttgta gcatttagta atcgctactt atttaattca
     1561 gattttggtg aaaagatggg aatgaaattc catacagatg tcatgggtga cgtgacaact
     1621 aacattggtg ttattacata tgataatgaa aacgcaggtc ttttcggtat caacttacgc
     1681 tacccagaag gatttgaatt tgaaaaagct atggatcgtt ttgcaaatga gattcaacaa
     1741 tatggctttg aagtgaaatt aggtaaagtc caaccaccac attatgttga taaaaatgat
     1801 ccttttgtac aaaagttagt tactgcatat agaaatcaaa caaatgatat gactgaacct
     1861 tatactatag gtggcggtac ttatgcgaga aacttagaca agggtgtagc atttggcgca
     1921 atgtttagtg attctgaaga tttaatgcat cagaaaaatg aatatatcac taaaaaacag
     1981 ttatttaacg caactagtat ttacttagaa gcaatttatt cattatgcgt ggaggaataa
     2041 tatatggaaa aaattttttt aaatggtgag tttgtaagtc caagtgaagc aaaggtttca
     2101 tacaacgaca gaggatacgt atttggcgat ggtatttatg aatacattcg agtatataat
     2161 ggtaagttat ttacagtaac agaacattat gaaagatttt tacgtagtgc caatgagatt
     2221 ggtttagatt taaattattc tgtagaagaa ttaattgaac tatctcgtaa attagttgat
     2281 atgaatcaaa ttgaaactgg ggcaatttat attcaagcaa cgcgtggtgt agctgaaagg
     2341 aatcatagct tcccgacacc tgaagtagaa ccagcaattg ttgcttatac aaagagttat
     2401 gatcgtcctt atgatcattt agaaaatggt gtgaatggtg ttaccgttga agatatccga
     2461 tggttacgtt gcgacattaa aagcttgaac ttattaggaa atgtattagc aaaagaatat
     2521 gctgtgaaat ataatgcagt tgaagcaatt caacatcgag gtgaaactgt aactgaagga
     2581 tcttcaagta atgcttatgc aattaaagac ggtgtgattt atacacatcc gattaacaac
     2641 tatattctta atggtattac acgaattgta attaaaaaaa ttgccgaaga ctataacatc
     2701 ccatttaaag aagaaacgtt tactgtagat ttcttgaaaa acgcagatga agttattgtt
     2761 tcaagtactt cagctgaggt tacacctgtt attaaattag atggtgaacc agttaatgat
     2821 ggtaaagttg gcccaattac acgtcaacta caagaaggat ttgaaaagta tatagagtca
     2881 cacagtattt aaaattcttt catcatattt ttagattaaa ttacttaaat ttatcaactt
     2941 tcgtaagtga attatgttat aatttaaaac gaagcttaaa agtgttgttt agaaaacaca
     3001 tattagtaaa aagtgccatt ttgtttctca ttttcagttg aaatataatg ataaaaatta
     3061 taaaattttc tatgagttac aaatatgatg tgcatcatat tttactaagt acgtaaatgt
     3121 tacagttcat aaatttatgc taaccatgaa aatgggatag caattatttt gaaaactaga
     3181 gtttatcgcc tacagagatt caaaaacatg tagttttaga cacggtaaca ttatatgtca
     3241 agtaagtgac tatagaatgt atggtgtcaa tgacagacat tacgatttaa atttagtcgt
     3301 gtaatgtagg cattggttga ggatttacaa taattatctg gacataatat ttgtaccagt
     3361 atggtgttat aaattagaat atatggctct tgaaaaacct atttctagag ccattttcta
     3421 attgaaagtg aggtggacga gttggagcag ttttatcaat tagggtggac acttgattca
     3481 gcaggtggtg catctggtga agcatatatg gctgaacaag atggacaaaa gttgttttta
     3541 aaacgaaatt caaatccatt tattgcggca ttatcagcag aaggtattgt gcccaaatta
     3601 gtatggacga aacgcataga aacaggcgag gttgttacag cacaacattg gaaaaatggg
     3661 cgtgaactat cttcaaacga aatgaagcaa acaagagttg cacatttatt aaagaagata
     3721 cacaattcta gacctttatt aagtatgtta aagcgtatgg aaatggaacc tattactcct
     3781 gagattatgc ttaataaaat taatgcctct ttatcaagag aagttttaac acatcatatt
     3841 gtgagaaaat cattaaccta tttagaagag catataccga gtttagattc gcgttttttc
     3901 actgttgtac atggtgatgt gaatcataat aattggttat tatccgatcg agatgaacta
     3961 tttttagttg attgggaagg tgcaatgatt gcagacccgg caattgatat cggaatgctg
     4021 ctttacaact atgttccaca acaacaatgg tctgaatggc ttgaaacata tggtgttcaa
     4081 gagagcttga atttaaataa acgtatgaaa tggtatacgg taattcaatc tataggtctt
     4141 gttcaatggt atgaagagca aaaaagatat aaggatatga acacttggct taaattccta
     4201 aatgaagtta tgaatagtaa catgtttatt taagaggaga ataaaatatg agagttcgat
     4261 acaaaccatg ggctgaagat tatttaaaag atcatcctga attggttgac atggacggtc
     4321 aacatgcagg gaaaatgact gaatggtttg ataaaacgca accgatacac attgaaattg
     4381 gttcgggtat ggggcagttt attacaacat tagctgctca aaatcctcat attaactata
     4441 tttcaatgga acgtgagaaa agtatcgttt ataaagtatt ggataaagtt aaagagatgg
     4501 gtttaacaaa tttaaagatt atttgtaatg acgccattga attaaatgaa tattttaaag
     4561 acggcgaagt ttcgcgtatt tatttgaact tttcagatcc atggccaaaa aatcgtcatg
     4621 caaaacgacg tttaacgtat catacgtttt tagcgttgta tcaacaaatc ttaaatgatg
     4681 aaggagattt acattttaaa actgataaca gaggactatt tgcctacagt ttagaaagta
     4741 tgtctcaatt tggaatgtat tttacaaaaa taaatctaaa tttacatcaa gaagacgatg
     4801 gcagtaatat tttaacagaa tatgaaaaga aattttcgga taaagggtca cgtatttatc
     4861 gtatggaagc gaaattccat tcacaaaaat agagcactta tgagggtatg tacacataca
     4921 atcataagtg ttttttcgta ctataatggt tctatagata aaggttctat aataaatcgg
     4981 actgatggaa aaagttttta acttttcatc tgtccgattt tttgattttg aatataaaaa
     5041 aagcgcaatt atctctataa ttaaaagtga ctaaacaaat aactaaagga gataatgcgc
     5101 ctatgaacaa tgttatacta aatacctttg atttaaaaga ataaagcttg cattttactt
     5161 gtaaatttta attattaatt taaaaataca ggccgatggt tatgatgcat ttacatataa
     5221 tatgattgca ttaatcttta taagtagtta gaagtgtatt agtgtggggc gtttcgtttt
     5281 tgttcgcatt tatgaatagt gatatgatgt aataaacaca tgctaattgt aataacgaat
     5341 acagaaagaa taggtgatgt tatgaaaatc ggggatatat ctattcatta tctaaatggt
     5401 ggcaatacaa aaatggatgg cggtgcaatg tttggtgttg ttccgaagcc gttgtggtca
     5461 aagcaataca atgcaaatga acgaaatcaa atcaatttac cgacacatcc aattttgatt
     5521 caaacggctc aatataattt gattatagat gcaggtattg gtaatggtaa attatctgaa
     5581 aagcaattac gtaattttgg agtagatgaa gaaagtcata taattgctga tttggcaaat
     5641 tataatttaa cgccaaagga tattgattat gtgctaatga cacatatgca ttttgatcat
     5701 gctgccggtt tgactgatca agcaggacat gcaatttttg aaaatgcgat tcatgttgtg
     5761 caacaagatg agtggcatga gtttattgca cctaatataa gaagtaaatc aacttactgg
     5821 gataagaata aaggcgatta tagtaacaag ttgattttat tcgaaaaaca ttttgaaccg
     5881 gttctgggta tcaagatgca acatagtgga ggtcatagct ttggccacac gattattacg
     5941 attgaaagtc aaggagataa agcagttcat atgggtgata tattcccaac tactgcacat
     6001 aaaaatcctc tatgggtaac ggcatatgat gattatccta tgcaatcgat tcgtgaaaaa
     6061 gaacgcatga taccatattt tattcagcaa caatattggt tcttgtttta tcatgatgaa
     6121 aactactttg ctgtaaaata cagcgataat ggtgaaaaca tagatgcata tattttacgt
     6181 gaaacattag ttgataataa ctaaaataaa gatgtattac taaacaaatt ttcaaaaata
     6241 aaaaattgag ccacatccaa tcttactaat tagggtgtgg ctcattttta agttttacga
     6301 tccaaatcaa atatggataa aattcgtatt aacgctctac aatgttaatg acttcaccag
     6361 tatatgcatc tgcataaaaa tcataatgaa tattttgacc atttttaata gttgtaattc
     6421 caccttgata aactaaacgg tatttatcag tttcaggatg aacgaatggt tcataaacga
     6481 tatatgagcc tttgacattc ataaaatatg attttacttc attaagtact ttaacgggat
     6541 tatagcgttt acgattaata aattggatga ttgaaatggt agaaataatt acaatggcta
     6601 ctgcaattat tgttggaatt atatatttca gtttagtcat catttatgct cccttgattt
     6661 caaattcata tcattagttt accatattga agatgatata ataacttata aggagtgaga
     6721 acttttatga acataaataa aaaagtaaca ttacaacgaa ttcaaacttt aactgagctt
     6781 catggggcac caggttttga agaagaagta aaaaattata tgactcagca aatggcgccg
     6841 tacgtagatg aatttattga aaatcgtatg ggtggatttt ttggtgtgaa aaaatctaaa
     6901 aatccaaatg caaaacgtgt aatgattgca gcacatatgg atgaaatcgg atttatgatt
     6961 acaaatatca ctaaaaatgg aatgattcaa ttcacaaatt taggtggtgt tgcaaatgat
     7021 atttggcaag gacaacgctt agtaattaaa aatagaaatg gcgataaaat tatcggtgtt
     7081 gtttctaata tacctaaaca ttttcgtact ggtagtgaag gtgcaccgga aattaaagat
     7141 ttaacattag atataggtgc tcaaaatgaa gatgaggtgc gtgagcgcgg aatagatata
     7201 ggagatacaa ttgtacctca cacgccattc acacagttat ctgaacatcg atatagtgct
     7261 aaagcatggg ataatcgtta tggttgtgtc ttggcaattg aaatactaga attattaaaa
     7321 gatatagaat tagatgtaga cttgtatgtt ggcgcaaatg ttcaagaaga ggttggatta
     7381 cgaggtgcga aagcatctgc agagatgata gacccagacg ttgcatttgt agttgattgt
     7441 tcacctgcca atgacgttaa aggaaaccaa ccattatctg gtgaacttgg taaagggacg
     7501 ttaattcgca taaaagacgg tacaatgatt ttaaagcctg tatttagaga ctatttatta
     7561 aagttagtag aagcacatga cattgaacat caatactata tgtcaccagg tggaacagat
     7621 ggtggagaaa ttcataaagc taatattggt attccgactg cagttattgg tgtatgtgca
     7681 cgatatattc atagtacaga ctcagtattt gatataagag actattttgc agctagatct
     7741 ttactttcag aagccatttg taatttagat aataatcaaa tagaaacatt acaatataaa
     7801 taatcgggta ataacaacta ttatctctaa atagttatat ataatcatta attaaggaga
     7861 cataaaaatg aaacaacttg aatcagaaca acaatttgaa tctttaaaac aaggtgctac
     7921 agtatttgaa ttcactgcag gctggtgtcc agattgtaga gtgatagaac cagatttacc
     7981 ggaattagaa gcgagatatc ctatgtttga cttcgtatca gtagaccgtg ataaatttat
     8041 ggatatttgt attgaaaatg gtattatggg tattccaagt tttctagtat ataaaaatgg
     8101 agaactgctt ggaagttata ttggaaaaga acgaaaatca attgaacaga tagatgcatt
     8161 tttagctcaa tacgtgtaat ttagactaga gaaaaacggg gtaatactcg tttttctctg
     8221 ttactatgtg ttgatttatt gtaaactatt ataaggtgcg aaattaggag tgttacatat
     8281 gaataccttt caaatgagag ataaattaaa ggaacgttta agccatttag acgttgattt
     8341 taaatttaat cgtgaagaag aaactttgcg tatttatcga acagataata acaaaggtat
     8401 cacgattaaa cttaacgcta tagtcgcaaa atatgaagat aaaaaagaaa aaattgtaga
     8461 tgaaattgtt tattacgttg atgaagctat tgcacaaatg gcagataaaa cccttgagag
     8521 tatatcatct agtcaaatta tgcctgtcat tagagcgact agctttgata aaaaaactaa
     8581 acaaggtgtt ccttttatct atgatgagca tactgcagaa acagcagttt attatgcagt
     8641 cgatttaggg aaatcatatc gtctaattga cgaaagcatg ttagaggatt tgaagttaac
     8701 tgaacaacaa ataagagaaa tgtctctgtt taatgttaga aaattgtcaa attcatatac
     8761 gactgatgaa gtaaaaggta atatttttta ttttattaac tcaaatgacg ggtatgatgc
     8821 aagtaggata ctaaatactg catttttaaa tgaaattgag gcacaatgtc aaggcgaaat
     8881 gctcgtagca gtgccacacc aagatgtgtt aattattgca gatatacgca ataaaacagg
     8941 atatgatgtg atggcacatt taacaatgga atttttcact aaaggtctag ttccaattac
     9001 atcattatcc tttggatata aacagggtca tcttgaaccg atatttattt taggtaaaaa
     9061 taataaacaa aaaagagatc caaacgtgat tcagcgttta gaagcaaatc gtcgtaaatt
     9121 taataaagat aaatagaaat aattggataa ggagttttgt cataatgaat ttattttaca
     9181 atcctaaata tgtaggagat gtcgcatttt tacaaattga accagttgaa ggtgaattaa
     9241 actacaataa aaaaggtaat gttgttgaaa ttactaatga aggtaatgtt gtaggttata
     9301 atatttttga aatttcaaaa gatataacaa ttgaagaaaa aggtcatatt aaattaactg
     9361 atgaacttgt aaatgtattc caaaagcgta tttcagaagc tggttttgat tataaattaa
     9421 atgctgatct atcaccgaaa tttgtagttg gctacgttga aactaaagac aaacatcctg
     9481 atgcagataa attaagtgta ctaaatgtaa acgttggaaa tgacacatta caaattgtat
     9541 gtggcgcgcc taacgttgaa gctggacaga aagttgttgt tgctaaagta ggtgcagtga
     9601 tgcctagcgg tatggtaatt aaagatgctg aattacgtgg tgttgcctca agcggtatga
     9661 tttgttcaat gaaagaattg aatttaccta atgcacctga agaaaaaggt attatggtat
     9721 taaatgacag ctatgaaatt ggacaagcat tttttgaata attaaggaag gtagtgaaaa
     9781 tatgagctgg tttgataaat tattcggcga agataatgat tcaaatgatg acttgattca
     9841 tagaaagaaa aaaagacgtc aagaatcaca aaatatagat aacgatcatg actcattact
     9901 gcctcaaaat aatgatattt atagtcgtcc gaggggaaaa ttccgttttc ctatgagcgt
     9961 agcttatgaa aatgaaaatg ttgaacaatc tgcagatact atttcagatg aaaaagaaca
    10021 ataccatcga gactatcgca aacaaagcca cgattctcgt tcacaaaaac gacatcgccg
    10081 tagaagaaat caaacaactg aagaacaaaa ttatagtgaa aaacgtggga attctaaaat
    10141 atcacagcaa agtataaaat ataaagatca ttcacattac catacgaata agccaggtac
    10201 atatgtttct gcaattaatg gtattgagaa ggaaacgcac aagccaaaaa cacataatat
    10261 gtattctaat aatacaaatc atcgtgctaa agattcaact ccagattatc acaaagaaag
    10321 tttcaagact tcagaggtac cgtcagctat ttttggcaca atgaaaccta aaaagttaga
    10381 aaatggtcgt atccctgtaa gtaaaccttc agaaaaagtt gagtcagata aacaaaaata
    10441 tgataaatat gtagctaaga cgcaaacgtc tcaaaataaa caattagaac aagaaaaaca
    10501 aaatgatagt gttgtcaaac aaggaactgc atctaaatca tctgatgaaa atgtatcatc
    10561 aacaacaaaa tcaatgccta attattcaaa agttgataat actatcaaaa ttgaaaatat
    10621 ttatgcttca caaattgttg aagaaattag acgtgaacga gaacgtaaag tgcttcaaaa
    10681 gcgtcgattt aaaaaagcgt tgcaacaaaa gcgtgaagaa cataaaaacg aagagcaaga
    10741 tgcaatacaa cgtgcaattg atgaaatgta tgctaaacaa gcggaacgct atgttggtga
    10801 tagttcatta aatgatgata gtgacttaac agataatagt acagatgcta gtcagcttca
    10861 tacaaatggc atagagaatg aaactgtatc aaatgatgaa aataaacaag cgtcaataca
    10921 aaatgaagac actaatgaca ctcatgtaga tgaaagtcca tacaattatg aggaagttag
    10981 tttgaatcaa gtatcgacaa caaaacaatt gtcagatgat gaagttacgg tttcgaatgt
    11041 aacgtctcaa catcaatcag cactacaaca taacgttgaa gtaaatgata aagatgaact
    11101 aaaaaatcaa tccagattaa ttgctgattc agaagaagat ggagcaacga ataaagaaga
    11161 atattcagga agtcaaatcg atgatgcaga attttatgaa ttaaatgata cagaagtaga
    11221 tgaggatact acttcaaata tcgaagataa taccaataga aacgcgtctg aaatgcatgt
    11281 agacgctcct aaaacgcaag agtacgcagt aactgaatct caagtaaata atatcgataa
    11341 aacggttgat aatgaaattg aattagcacc gcgtcataaa aaagatgacc aaacaaactt
    11401 aagtgtcaac tcattgaaaa cgaatgatgt gaatgataat catgttgtgg aagattcaag
    11461 catgaatgaa atagaaaaga ataacgcaga aattacagaa aatgtgcaaa acgaagcagc
    11521 tgaaagtgaa caaaatgtcg aagagaaaac tattgaaaac gtaaatccaa agaaacagac
    11581 tgaaaaggtt tcaactttaa gtaaaagacc atttaatgtt gtcatgacgc catctgataa
    11641 aaagcgtatg atggatcgta aaaagcattc aaaagtcaat gtgcctgaat taaagcctgt
    11701 acaaagtaag caagctgtga gtgaaagaat gcctgcgagt caagccacac catcatcaag
    11761 atctgattca caagagtcaa atacaaatgc atataaaaca aataatatga catcaaacaa
    11821 tgttgagaac aatcaactta ttggtcatgc agaaacagaa aatgattatc aaaatgcaca
    11881 acaatattca gagcagaaac cttctgttga ttcaactcaa acggaaatat ttgaagaaag
    11941 tcaagatgat aatcaattgg aaaatgagca agttgatcaa tcaacttcgt cttcagtttc
    12001 agaagtaagc gacataactg aagaaagcga agaaacaaca catccaaaca atactagtgg
    12061 acaacaagat aatgatgatc aacaaaaaga tttacagtca tcattttcaa ataaaaatga
    12121 agatacagct aatgaaaata gacctcggac gaaccaacaa gatgttgcaa caaatcaagc
    12181 tgtacaaaca tctaagccga tgattcgtaa aggcccaaat attaaattgc caagtgtttc
    12241 attactagaa gaaccacaag ttattgagtc ggacgaggac tggattacag ataaaaagaa
    12301 agaactgaat gacgcattat tttactttaa tgtacctgca gaagtacaag atgtaactga
    12361 aggtccaagt gttacaagat ttgaattatc agttgaaaaa ggtgttaaag tttcaagaat
    12421 tacggcatta caagatgaca ttaaaatggc attggcagcg aaagatattc gtatagaagc
    12481 gcctattcca ggaactagtc gtgttggtat tgaagttccg aaccaaaatc caacgacagt
    12541 caacttacgt tctattattg aatctccaag ttttaaaaat gctgaatcta aattaacagt
    12601 tgcgatgggg tatagaatta ataatgaacc attacttatg gatattgcta aaacgccaca
    12661 cgcactaatt gcaggtgcaa ctggatcagg gaaatcagtt tgtatcaata gtattttgat
    12721 gtctttacta tataaaaatc atcctgagga attaagatta ttacttatcg atccaaaaat
    12781 ggttgaatta gctccttata atggtttgcc acatttagtt gcaccggtaa ttacagatgt
    12841 caaagcagct acacagagtt taaaatgggc cgtagaagaa atggaacgac gttataagtt
    12901 atttgcacat taccatgtac gtaatataac agcatttaac aaaaaagcac catatgatga
    12961 aagaatgcca aaaattgtca ttgtaattga tgagttggct gatttaatga tgatggctcc
    13021 gcaagaagtt gaacagtcta ttgctagaat tgctcaaaaa gcgagagcat gtggtattca
    13081 tatgttagta gctacgcaaa gaccatctgt caatgtaatt acaggtttaa ttaaagccaa
    13141 cataccaaca agaattgcat ttatggtatc atcaagtgta gattcgagaa cgatattaga
    13201 cagtggtgga gcagaacgct tgttaggata tggcgatatg ttatatcttg gtagcggtat
    13261 gaataaaccg attagagttc aaggtacatt tgtttctgat gacgaaattg atgatgttgt
    13321 tgattttatc aaacaacaaa gagaaccgga ctatctattt gaagaaaaag aattgttgaa
    13381 aaaaacacaa acacaatcac aagatgaatt atttgatgat gtttgtgcat ttatggttaa
    13441 tgaaggacat atttcaacat cattaatcca aagacatttc caaattggct ataatagagc
    13501 agcaagaatt atcgatcaat tagagcaact cggttatgtt tcgagtgcta atggttcaaa
    13561 accaagggat gtttatgtta cggaagcaga tttaaataaa gaataattat gagtaaggag
    13621 ttttatataa tgacacacta tcattttgtc ggaattaaag gttctggcat gagttcatta
    13681 gcacaaatca tgcatgattt aggacatgaa gttcaaggat cggatattga gaactacgta
    13741 tttacagaag ttgctcttag aaataagggg ataaaaatat taccatttga tgctaataac
    13801 ataaaagaag atatggtagt tatacaaggt aatgcattcg cgagtagcca tgaagaaata
    13861 gtacgtgcac atcaattgaa attagatgtt gtaagttata atgatttttt aggacagatt
    13921 attgatcaat atacttcagt agctgtaact ggtgcacatg gtaaaacttc tacaacaggt
    13981 ttattatcac atgttatgaa tggtgataaa aagacttcat ttttaattgg tgatggcaca
    14041 ggtatgggat tgcctgaaag tgattatttc gcttttgagg catgtgaata tagacgtcac
    14101 tttttaagtt ataaacctga ttacgcaatt atgacaaata ttgatttcga tcatcctgat
    14161 tattttaaag atattaatga tgtttttgat gcattccaag aaatggcaca taatgttaaa
    14221 aaaggtatta ttgcttgggg tgatgatgaa catctacgta aaattgaagc agatgttcca
    14281 atttattatt atggatttaa agattcggat gacatttatg ctcaaaatat tcaaattacg
    14341 gataaaggta ctgcttttga tgtgtatgtg gatggtgagt tttatgatca cttcctgtct
    14401 ccacaatatg gtgaccatac agttttaaat gcattagctg taattgcgat tagttattta
    14461 gagaagctag atgttacaaa tattaaagaa gcattagaaa cgtttggtgg tgttaaacgt
    14521 cgtttcaatg aaactacaat tgcaaatcaa gttattgtag atgattatgc acaccatcca
    14581 agagaaatta gtgctacaat tgaaacagca cgaaagaaat atccacataa agaagttgtt
    14641 gcagtatttc aaccacacac tttctctaga acacaggcat ttttaaatga atttgcagaa
    14701 agtttaagta aagcagatcg tgtattctta tgtgaaattt ttggatcaat tagagaaaat
    14761 actggcgcat taacgataca agatttaatt gataaaattg aaggtgcatc gttaattaat
    14821 gaagattcta ttaatgtatt agaacaattt gataatgctg ttattttatt tatgggtgca
    14881 ggtgatattc aaaaattaca aaatgcatat ttagataaat taggcatgaa aaatgcgttt
    14941 taatatgttt ataatagagt agtatgggta tttattatta atgacattat tacatgttaa
    15001 ttaggaggcg tttttaatgg attggatttt accaattgct ggaattatcg ctgcgattgc
    15061 attcttaatt ttatgtatcg gtatcgtagc tgtattaaat tctgttaaga aaaacttaga
    15121 ttatgttgca aaaacacttg acggtgtaga aggtcaagtt caaggtatta ctcgtgaaac
    15181 aacagattta cttcataaag taaaccgttt aactgaggat atccaaggta aagtagatcg
    15241 tttaaactca gttgtagatg ctgttaaagg tatcggtgac tcagtacaaa cgttaaacag
    15301 ctctgtagat cgtgtaacaa attcaattac acataatatt tctcaaaatg aagataaaat
    15361 ctcacaagtt gttcaatggt caaatgttgc aatggaaatt gcagacaaat ggcaaaatag
    15421 acactaccgt cgtggaagtg caaattacaa agctaataat gtagcaactg atgcaaatca
    15481 tagctatact tctagagtag ataaataatt aataattaaa caaatgcact taaatgaggt
    15541 tgttacttat ttaagtgcat ttgttatagg aggatataca aatgacgcag caacaaaatg
    15601 ataaaagaac attaaaaaat aaacacactt atcaaaatga accactacca aaccgtaaag
    15661 attttgttgt tagttttata actggcgcgc ttgttggttc agctttaggt ttgtatttta
    15721 aaaataaagt ttatcaaaaa gcagatgatt taaaagtcaa agaacaagaa ctgtcgcaaa
    15781 agtttgaaga aagaaaaacg caacttgaag aaacggttgc ctatacaaaa gaacgtgttg
    15841 aaggattttt aaacaaatct aaaaatgagc aagcggcatt aaaggcacaa caagcagcga
    15901 taaaagaaga agcaagtgca aataatttaa gtgatacatc acaagaagca caagagattc
    15961 aagaagctaa aagagaagca caagcagaag cggataaaag tgtggctgta tcaaataaag
    16021 aatcaaaagc agtggcattg aaagcacaac aagcagcgat aaaagaagaa gcaagtgcaa
    16081 ataatttaag tgatatatca caagaggcac aagaggttca agaagctaaa aaagaagcac
    16141 aagcagagaa agacagtgac acattaacta aagatgcaag tgcagcaaag gtagaagtat
    16201 caaaaccaga gtcacaagct gaaagattag caaacgctgc aaaacagaag caagctaaat
    16261 taacaccagg ttcaaaagag agtcaattaa ctgaagcgtt atttgcagaa aaaccagttg
    16321 ctaaaaatga cttgaaagaa attcctcaat tagttactaa aaagaatgat gtatcagaga
    16381 cagagacggt taatatagat aataaagaca ctgttaaaca aaaagaagct aaatttgaaa
    16441 atggtgttat tacacgtaaa gctgatgaaa aaacaactaa taatacagct gttgacaaga
    16501 aatcaggtaa acaatctaaa aaaacaacac cttcaaataa acgaaatgca tcaaaagcat
    16561 ctacaaataa aacttcaggt cagaaaaagc aacataataa gaaatcatca caaggtgcaa
    16621 agaaacaaag tagttcaagt aagtcaactc aaaagaataa tcaaactagt aataagaatt
    16681 caaaaacaac aaatgctaaa tcatccaatg catcaaaaac gccaaatgct aaagttgaga
    16741 aagctaaaag taaaatagag aaacgtacat tcaatgacta agattactaa aatcgttgaa
    16801 taatcaatca tagcagtaag ctatgtaata tatttttaaa aattgaattt caaaataaaa
    16861 ggtaggcatt caggtgctta ccttttttga tatcatcagt ttattttgaa aggcaatgcg
    16921 atcatttcat gtatttatgt tgtttgaaac atcgttagat aacaatagtg atattgcaca
    16981 ttttaagctg aagatggaaa aatattcgat aatcaaataa acaatgaatt ttagaaggta
    17041 caatgacgtt cactaattta aatatagctg aatgtgttgg tgagtgatgt tcactataga
    17101 tttatattaa tatacaaaga caaaggttgt taatttttat taagcgttag gttgaatgta
    17161 tgagaatttt agatttataa tagaagatag aaacgaaaat ttttcttaaa agcagtaatg
    17221 ttgactcaaa ataagctata ataatgacac ttatttaatt gattaacatt tgctaataaa
    17281 tatcaatata gaatataact ttccaataat gactgagaaa atcgaaatgt cagtctcgaa
    17341 tcatataatt agaaaattga ttattttctg tcaatttagg gttgaactat acatatgata
    17401 ttgttagaat attttttaac attatatttt attgctttaa agtggaatat acttgaaata
    17461 attagtagag gtgagtaagg atgagtaata aattagaatc atacagaagt gagattgtat
    17521 cactgaatca tcaaatttta gacttattat ctaaacgtgg tgaactagca caaaaaattg
    17581 gggaagaaaa attaaaacaa ggtacacgta tttatgatcc acaacgtgaa aaagaaatgc
    17641 ttaacgactt aatcgatagt aacaaaggac cattcaacga taatactatt aagcaattat
    17701 ttaaagaaat tttcaaagcc tctacagatt tacaaaaatc tgaaaatgaa aaacatttat
    17761 atgtatcacg taagttgaaa cctgaagata cgattgtaac atttgataat gggggcatta
    17821 ttggagacgg caataaatca tttgtatttg ggccatgttc agttgaatca tttgaacaag
    17881 ttgaagctgt tgctaaaaac ttacatgcta aaggtgaaaa atttattcgt ggcggtgcat
    17941 ttaaaccacg tacatcacca tatgatttcc aaggcctagg tgttgaagga cttaaaatac
    18001 ttaaacagat taaagataaa tatgatttaa atgttgtcag cgaaatcgta aatccaaatg
    18061 attttgaagt ggctgatgag tatttagacg tattccaaat tggtgcacgt aatatgcaaa
    18121 acttcgagtt attaaaagaa gctggccgta cgaaaaagcc tattctatta aaacgtggtt
    18181 tatctgctac aatcgaagag tttgtttatg cagctgaata cattgcttca caaggtaatc
    18241 aaaacattat tttatgtgaa cgtggaatcc gaacttatga aaaggcgaca cgtaacactt
    18301 tagatatttc agcagtacca attttaaaac aaggtacaca cttaccagtc atggtagatg
    18361 ttacgcatag tacaggtcgt aaagatatca tgttaccaac tgcgaaagca gcattagcag
    18421 ttggtgctga tggagttatg gctgaggtgc atccagatcc atctgttgca cttagtgatg
    18481 cgggtcaaca aatggattta gatgaattcc aagcatttta tgatgaatta aagcctttag
    18541 ctgatttata taacgctaaa aagttaaaat aatattccaa ggaaactata gactacttaa
    18601 ctaatatgtc atgttgaagt agaatattat ctttgaatcg acaattttaa acttacagcc
    18661 attctaagag tatattactt ttagagtggc tattattttt tgtatagaaa taaaggtata
    18721 ctgcacttaa cgattgttat aatacttcga cacttgttca atttcacaat tattaaagat
    18781 tatgactgat agcagtaatt aaaattataa ctatgaatta tctgtaaaat ataatagatt
    18841 cacacatttg ttgctgaaat gtgaacattt ttcaacaaat gcaattgata t
//
LOCUS       DALAGF010000046        18751 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.046, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000046 DALAGF010000000
VERSION     DALAGF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18751
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.046"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..453
                     /locus_tag="O2692_001472"
     CDS             <1..453
                     /locus_tag="O2692_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCZ4299105.1"
                     /translation="LYKFLKENEKQYQYTGFTKEAINKTQNVGYQNEYFYITYLSRNL
                     KEYRKYYEPLIHKNDKEFKEGMQRARKELNYTANTNTVATLFSTNDERNRKEKINNVI
                     DLSEKIERTKDMPIKNTITTQLGNKLIGTKKARFDDKKVVSFGAFEDE"
     gene            446..652
                     /locus_tag="O2692_001473"
     CDS             446..652
                     /locus_tag="O2692_001473"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299106.1"
                     /translation="MNKINDRDLTELSSYRVYQDINKDNDFTVNEKRFKQADVFEDLY
                     REKLKDTNKLREYNYLQNETFKSA"
     gene            667..1581
                     /locus_tag="O2692_001474"
     CDS             667..1581
                     /locus_tag="O2692_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCZ4299107.1"
                     /translation="MLKKAKFILMATILLSGCSTTNNESNKETKSVPEEMDASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVIGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSKDKGDDMSTLVGAVLSGFEYRAQKEKYDKLYKFFK
                     DNEEKYQYTGFTKEAINKTQNSGYENEYFYISAIPYNLAEYRDYFEPLLNKSDSEFSK
                     ELSNVKKQLKDKSKVSVTTTLFSKKKNYTKKSNSENVIKMAEEIKKDKEIPNGIELSI
                     KFSDNKINTVKPNFNGESTSEYGVFDQE"
     gene            1780..1899
                     /locus_tag="O2692_001475"
     CDS             1780..1899
                     /locus_tag="O2692_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299108.1"
                     /translation="MTIFTQLSDRIKKAISKINQANNIPNNARKSPMYHSPIT"
     regulatory      1982..2116
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2178..2723
                     /locus_tag="O2692_001476"
     CDS             2178..2723
                     /locus_tag="O2692_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCZ4299109.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(2829..3077)
                     /locus_tag="O2692_001477"
     CDS             complement(2829..3077)
                     /locus_tag="O2692_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCZ4299110.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            3185..4138
                     /locus_tag="O2692_001478"
     CDS             3185..4138
                     /locus_tag="O2692_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ4299111.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            4128..5507
                     /locus_tag="O2692_001479"
     CDS             4128..5507
                     /locus_tag="O2692_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCZ4299112.1"
                     /translation="MLHDILRNKFGFESFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQAVLTTLQSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            5660..7120
                     /gene="ebpS"
                     /locus_tag="O2692_001480"
     CDS             5660..7120
                     /gene="ebpS"
                     /locus_tag="O2692_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HCZ4299113.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHDTIKNAENNTEHSTVSDKSEA
                     EQSQQPKPYFTTGANQSETSKNEHDNDSVKQDQDEPKEHHNGKKAAAIGAGTAGVAGA
                     AGAMAASKAKKHSNDAQNKSNSGKANNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            7245..7361
                     /locus_tag="O2692_001481"
     CDS             7245..7361
                     /locus_tag="O2692_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299114.1"
                     /translation="MLFNKVFSRILAYQFNFLNSHQKDKTPQSTNLTRKNKK"
     gene            7522..8508
                     /locus_tag="O2692_001482"
     CDS             7522..8508
                     /locus_tag="O2692_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HCZ4299115.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(8623..9591)
                     /locus_tag="O2692_001483"
     CDS             complement(8623..9591)
                     /locus_tag="O2692_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCZ4299116.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            9668..10327
                     /locus_tag="O2692_001484"
     CDS             9668..10327
                     /locus_tag="O2692_001484"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCZ4299117.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
     gene            10358..10474
                     /locus_tag="O2692_001485"
     CDS             10358..10474
                     /locus_tag="O2692_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299118.1"
                     /translation="MQRLTHIQNECADNDLLKLKGDFYSMMNESCLFIFGKS"
     gene            10591..10782
                     /locus_tag="O2692_001486"
     CDS             10591..10782
                     /locus_tag="O2692_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299119.1"
                     /translation="MSVTNLNIAKEADLVTKNVLCNNSRKMHYIRSSYYSNVKVAYLR
                     GFLFYKSIKFYYNISCREQ"
     gene            11039..12214
                     /gene="rpsA"
                     /locus_tag="O2692_001487"
     CDS             11039..12214
                     /gene="rpsA"
                     /locus_tag="O2692_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCZ4299120.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
     gene            12436..13746
                     /gene="der"
                     /locus_tag="O2692_001488"
     CDS             12436..13746
                     /gene="der"
                     /locus_tag="O2692_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCZ4299121.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEAYVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTGAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            13763..14761
                     /locus_tag="O2692_001489"
     CDS             13763..14761
                     /locus_tag="O2692_001489"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCZ4299122.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            14932..15204
                     /locus_tag="O2692_001490"
     CDS             14932..15204
                     /locus_tag="O2692_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCZ4299123.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
     gene            15635..16207
                     /locus_tag="O2692_001491"
     CDS             15635..16207
                     /locus_tag="O2692_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HCZ4299124.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDLYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            16210..16935
                     /locus_tag="O2692_001492"
     CDS             16210..16935
                     /locus_tag="O2692_001492"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HCZ4299125.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            16937..17896
                     /locus_tag="O2692_001493"
     CDS             16937..17896
                     /locus_tag="O2692_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCZ4299126.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            17988..18437
                     /gene="ndk"
                     /locus_tag="O2692_001494"
     CDS             17988..18437
                     /gene="ndk"
                     /locus_tag="O2692_001494"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCZ4299127.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
BASE COUNT         7037 a         2639 c         3374 g         5701 t
ORIGIN      
        1 ttatataaat ttttaaaaga aaatgaaaag caatatcaat atacaggttt tactaaagaa
       61 gcaattaaca agacgcaaaa cgtcgggtat caaaatgaat atttttatat tacatattta
      121 tcaagaaatt taaaagaata tagaaaatat tacgaaccat tgatacataa aaatgataaa
      181 gagtttaaag aaggtatgca acgagctaga aaagagctaa actatactgc taatacaaat
      241 actgtagcaa cgttgtttag tacgaatgat gaaaggaata gaaaagaaaa gataaataat
      301 gtaatagatt tatccgagaa aattgaaaga acaaaagata tgccaatcaa gaatactata
      361 actactcaat taggaaataa acttattggc acaaaaaaag ctcgttttga tgataagaaa
      421 gtagtgtcgt ttggagcatt tgaagatgaa taaaataaat gatagagatt taacagaatt
      481 gagtagctat agggtttatc aagacatcaa taaagataat gactttacag ttaacgaaaa
      541 acgatttaag caggcagatg tatttgaaga tttatataga gagaaactaa aagacacaaa
      601 taaattaaga gagtataatt atttacaaaa tgaaactttt aaaagcgcat aaataggtga
      661 tgagatatgc ttaaaaaagc aaaatttatc ttaatggcaa cgatactact atcaggatgt
      721 tcaactacca ataacgaatc caacaaagaa acaaaatctg taccagaaga aatggatgct
      781 tcaaaatatg taggacaagg attccaacca cctgcagaaa aagatgcgat tgaatttgca
      841 aagaagcata aagataaaat tgctaagcga ggcgaacaat tttttatgga taacttcggt
      901 ctaaaagtta aagctacaaa tgttataggt agtggcgatg gtgtagaagt attcgtgcat
      961 tgtgatgacc acgacatcgt atttaatgcg agtattccat ttgataaatc aattattgac
     1021 agtgatagct cattaagaag taaggataaa ggtgatgata tgagtacttt agttggtgca
     1081 gtactcagtg ggtttgaata tcgagcacaa aaagaaaaat atgataaatt atataaattt
     1141 ttcaaagata atgaagagaa atatcaatat acaggattta caaaagaagc aattaataag
     1201 acgcaaaata gtggttatga aaatgaatat ttttatattt cggccatacc ttataattta
     1261 gctgagtata gagactattt tgaaccattg ttaaacaaaa gtgacagtga attttcaaaa
     1321 gaattgtcaa atgttaagaa gcaattaaaa gataagtcta aagtttcggt aactactact
     1381 ctatttagta aaaaaaagaa ctatactaaa aaaagtaaca gtgaaaatgt aataaaaatg
     1441 gcagaagaaa taaaaaaaga taaagagata ccaaacggta tagagcttag tataaaattt
     1501 tcggacaata aaataaatac ggttaaacca aattttaacg gtgaaagcac ttcagaatat
     1561 ggtgtgtttg atcaagaata aaattaatga tgaaaattta acggagaata gtgtatattg
     1621 agtagatcaa gaataaaaag ataattctac tattgttgtg aaggcaaata agtagaagat
     1681 tttaagtgta atttctggtg atttaaataa taatataaat ggaagtactg atataaaact
     1741 ttttaaccta ctagattctt ataatttgct ttccatttta tgacgatttt tactcaattg
     1801 agtgatagaa tcaaaaaagc catctcaaaa attaatcaag caaacaacat tccaaacaat
     1861 gctcgcaaat caccaatgta tcactctcca attacgtaac tatgatttaa tttaagcata
     1921 gttattgagg ttttgtgata tatagtataa aattaatgag aattaaattt aataatgtaa
     1981 aattcatctt cggggtcggg tgtaattccc aaccggcagt aaataaagcc tgcgacctgc
     2041 tagtatgtat catattagtg gctgatctag tgagattcta gagccgacag tatagtctgg
     2101 atgggagaag atggaggttt tttgttgtgc aataatcctc ctattcttac gagatgaatg
     2161 gaaggagaaa attgaatatg caacaaaata aacgtcttat cacaataagt atgttgagcg
     2221 cgattgcgtt tgtgttaact tttatcaagt ttcctatacc atttttgcca ccatacttaa
     2281 ctttagattt tagtgatgta ccgtcactac tagctacatt tacgtttgga ccagttgccg
     2341 gtatcgtagt tgcactggtt aaaaatttat tgaactactt atttagtatg ggcgatccag
     2401 ttggaccatt tgctaacttt ttagcaggcg caagtttctt attaactgct tacgccatct
     2461 ataaaaataa acgttcaaca aaatctttga ttactggatt aatcattgca acaatcgtta
     2521 tgactatcgt gttgagtatt ttgaactatt tcgttctatt acctttgtac ggtatgatat
     2581 ttaacttagc tgatatcgca aataatctta aagtaatcat tgtttcagga attataccat
     2641 tcaatattat taaaggtatc gttatttcta ttgtatttat tttactatat agaaggcttg
     2701 cgaatttctt gaaaagaatt taatcaaatt aaagcaaaat aatatacaca taataataaa
     2761 aagcaggtga ctatcaataa acgatagctt gcctgctttt tctatagaac atttgtctaa
     2821 aaaatcaatt attcaaattt taaagcgtcc ccatcaaatg attcgtctgc aattttaata
     2881 gaatctgtag ggcatccatc aattgcatct tccatatctt catataattc ctcaggtact
     2941 tctgcagtac cttggttatc gtcaaggatt acgaaagcaa taccttcgtc gtcgtaatca
     3001 tatatatctg gcgctgctgc accgcatgca ccacatgcaa tacaagtatc catatcaacg
     3061 attgtatatt ttgccaatgt cttcgcctcc tttgataaaa atgctaaaat agtaatgtga
     3121 ctaaaatttt agacagcatc atttttattt tcaaattatc cgttttacag agtgagggtt
     3181 aaatttgcaa cacattataa aaacagcatt acaacaaaca tttaactata aaacaaataa
     3241 aagtatttac aatatcttag ttggtaagaa atctcaccaa accttttttg acgcttgtag
     3301 tcaacaacag ttgtcattat atcacagttt accactatta aaatatccgt cttttgagct
     3361 atttttagaa aaaatcaatg aatttaatgc tgaaatggaa atcatgttgc atcctagata
     3421 tacatttgaa agcatggggc aaacatttca agcaattcaa ctattagtgc aaaccatgtc
     3481 taataccaaa caacatgttt ttcattttgt accaatctct caaaataata agatacaaga
     3541 aactgtaaag attgtctata actacattaa agaaaaccaa ttacaaattg attttgaaaa
     3601 tgaactacat aacttgttta aagcaataac tttaaaaggg ccatgttact tacattatta
     3661 tttgcaaggc tatgatgaac caatgtatac gagacagcaa gttagtttaa tagaaaagct
     3721 atctcaacag caattgtttg aatacgaaat gaataattta gtgacaatga tgtttgaatt
     3781 agaaagtgga gaatatacta ttttatcaaa aataataatg aaacctacat tattaaatca
     3841 aacttatatt acttatacaa aattgcttga acaattcacg atggaagata tagcggctca
     3901 acaacaagtt aaaatcaata ctatcgaaga tcatgtactt gaaatcttaa tcaaaggtta
     3961 catgtctaat tacgatgatt atgttgaact agaagatcaa ctccagtttt tgaattttta
     4021 tcaacagcat cgtggcgaac gattaaaatt ttacaaagaa caatttgaca cgttatcata
     4081 ttttcaatta aaagtattaa tcgttggatt tgaaagaggt gatctgaatg ttgcatgata
     4141 ttttacgaaa caaatttgga ttcgagagtt ttaaaccggg acaacaggaa attatagaaa
     4201 gtataatgtc tcaacaacac actctaggta tacttccaac tggaagtgga aagagtttgt
     4261 gttatcaaat acctacgtat ttatcaggta agccgacatt aattatctca ccgttaatat
     4321 ctttaatgga tgaccaagtt atgcagttga aaataaatgg agaaaaacgt gtaacatgta
     4381 ttcactctgg tatggatgaa attgagaaaa agcataatat taaatgttta cgacatagcc
     4441 gcttcatctt tctaagtcca gaatttctcc tgcaaccgtc aaattttaaa ttaatatcta
     4501 tgatagactt tggcatgatt gttctagatg aagcacattg cctatctgaa tggggatatg
     4561 atttcagacc acattatgct ctaataggaa aagtaacaaa gcattttaaa gaagcggttg
     4621 tcttagcatt gacagcaact gcaccaccgc atttacaaga tgatttgacg gaaatgttag
     4681 cgattcaatt caatgttatt aaaactacaa tgaatcgccc aaatataagc tttaagcatc
     4741 ttaattttca tgatgatgaa gataaaattg aatggttgct gccgtttcta caacagtcgg
     4801 gaccaacgat tatttatgtc tcatcgaaaa agatgtgtct gaatttagcg caacttattt
     4861 atgattcagg ttttcttaca ggtatttatc atggtgatat gaattatcaa gagcgacaca
     4921 cagttcaaca acaattttta aataatgata ttccgattat agtcgcaacg agtgcttttg
     4981 gtatgggaat taataaaaaa gatattcgca caatcattca ctttcatctt tcaacaagtc
     5041 cttctaacta cattcaagaa attggccgtg cgggtcgcga tggtgaacta agtcaggcaa
     5101 ttagtttatt ccaaccggac gataaatata ttttagaaac gttattattt gcagatatga
     5161 taacagaaga agatgtacaa aatttcgaaa taggagaatt tttagctccc gataaacaag
     5221 ccgttttgac aacgttgcaa tcattctata gtatcggcgc cttgaaacag atatttaagc
     5281 aatcatttaa acgaaagcaa ttaggattct ttcgcatgat tggctattgc aaattggatc
     5341 aatgtagacg gaagtattta ttagaatttt tcggtgaata tccaccggca caagatcgat
     5401 gttgtgacaa tgattctaat ataactgata tcgcaatttt aaataagaag aaggtaatta
     5461 gaagtattgg atttgatgaa aagttgcaaa atttatttct cagatagtat tactttacta
     5521 aaagaaaatt gacaagctat aattagtgta tacacaattg aaaaatgatt gaaataattt
     5581 tgaaaaatat acataaacat atgtcatgtg ggtatatttt atgtaaaatc attgtaatag
     5641 aatagaaagg aagatggcta tgtctaataa ttttaaagat gactttgaaa aaaatcgtca
     5701 atcgatagac acaaattcac atcaagacca tacggaagat gttgaaaaag accaatcaga
     5761 attagaacat caggatacaa tagagaatac ggagcaacag tttccgccaa gaaatgccca
     5821 aagaagaaaa agacgccgtg atttagcaac gaatcataat aaacaagttc acaatgaatc
     5881 acaaacatct gaagacaatg ttcaaaatga ggctggcaca atagatgatc gtcaagtcga
     5941 atcatcacac agtactgaaa gtcaagaacc tagccatcaa gacagtacac ctcaacatga
     6001 agaggaatat tataataaga atgcttttgc aatggataaa tcacatccag aaccaatcga
     6061 agacaatgat aaacacgata ctattaaaaa tgcagaaaat aacactgagc attcaacagt
     6121 ttctgataag agtgaagctg aacaatctca gcaacctaaa ccatatttta caacaggtgc
     6181 taaccaatca gaaacatcaa aaaatgaaca tgataatgat tctgtaaaac aagatcaaga
     6241 tgaacctaaa gaacatcata atggtaaaaa agcagcagct attggtgctg gaacagcagg
     6301 tgttgcaggt gcagctggtg caatggctgc ttctaaagct aagaaacatt caaatgacgc
     6361 tcaaaacaaa agtaattctg gcaaggcgaa taactcgact gaggataaag cgtctcaaga
     6421 taagtctaaa gatcatcata atggcaaaaa aggtgcagcg atcggtgctg gaacagcagg
     6481 tttggctgga ggcgcagcaa gtaaaagtgc ttctgccgct tcaaaaccac atgcctctaa
     6541 taatgcaagc caaaaccatg atgaacatga caatcatgac agagataaag aacgtaaaaa
     6601 aggtggcatg gccaaagtat tgttaccatt aattgcagct gtactaatta tcggtgcatt
     6661 agcgatattt ggaggcatgg cattaaacaa tcataataat ggtacaaaag aaaataaaat
     6721 cgcgaataca aataaaaata atgctgatga aagtaaagac aaagacacat ctaaagacgc
     6781 ttctaaagat aaatcaaaat ctacagacag tgataaatca aaagaggatc aagacaaagc
     6841 gactaaagat gaatctgata atgatcaaaa caacgctaat caagcgaaca atcaagcaca
     6901 aaataatcaa aatcaacaac aagctaatca aaatcaacaa cagcaacaac aacgtcaagg
     6961 tggtggccaa agacatacag tgaatggtca agaaaactta taccgtatcg caattcaata
     7021 ctacggttca ggttcaccgg aaaatgttga aaaaattaga cgtgccaatg gtttaagtgg
     7081 taacaatatt agaaacggtc aacaaatcgt tattccataa tataactata taaattgtaa
     7141 ctgaactgct atatacaaac gtgcatatta taagtatctc taaatatttt ggagaactta
     7201 aattgcgctt ataagtatgt agcggttttt tcatttttca aagtttgtta tttaacaagg
     7261 tcttttctcg aatattggca tatcaattta actttttaaa tagtcatcaa aaagataaaa
     7321 caccacaatc aacaaattta acgaggaaga ataaaaaata aatcaacata ttaaattgta
     7381 gtgttattca actccgtagc taacaattct ctatgcacat taaacaaatt gtcaaaaata
     7441 tatcataaat cttcaagcac agacttagcg catcaatcac tgaactgtta taatagtttg
     7501 ggattaaagg aggccgaaac aatgcaaaaa gttgaaagta tcataattgg tggagggcca
     7561 tgcggattaa gtgcggctat tgaacaaaaa agaaaaggta ttgatacctt aattattgaa
     7621 aagggtaatg tcgttgaatc aatctacaat tatcctactc accaaacatt tttctcatca
     7681 agtgataaat taagtattgg ggacgtaccg tttatcgttg aagaaagtaa accaagacgt
     7741 aatcaagcgc tagtttatta ccgagaagtt gtaaaacatc atcaattaaa agtaaatgca
     7801 tttgaagaag tattaactgt taaaaaaatg aataataaat ttactattac tacgacgaaa
     7861 gatgtttatg aatgtcgatt tttaacaatc gcgacaggct attatggtca gcataataca
     7921 ttagaagttg aaggtgcgga tttacctaaa gtgttccatt attttaaaga ggcacatccg
     7981 tattttgatc aagatgttgt aattatcggt ggtaagaatt cggctatcga tgctgctttg
     8041 gagttggaaa aagctggtgc taacgtgacg gttctatatc gtggtggaga ttattcgcct
     8101 tcaattaaac cgtggatact tccaaatttc acagcattag taaatcatga aaaaattgac
     8161 atggaattta atgctaatgt tacccaaata actgaagata ctgtgactta tgaagtaaat
     8221 ggtgaaagta aaacgataca caatgattat gtatttgcga tgattggtta tcatcccgat
     8281 tatgaatttt taaaatctgt aggcattcaa attaatacaa atgaatttgg aacagcgcct
     8341 atgtataata aagaaacata cgaaacaaat atcgaaaatt gctatattgc aggtgtaatt
     8401 gctgcaggga acgatgcgaa taccattttt attgaaaatg gtaaattcca cgggggcatt
     8461 attgctcaaa gcatgctagc taagaaacaa acgcccttag aatcataaaa ataaaggtct
     8521 atgtactaaa taacttagtt ttacaacgac tgacattcat gatatgtcaa atgaggttga
     8581 tgactaatga ttgtacatag acctttttat gttacgtatt cattataatt caaaatatga
     8641 tttgatttca gctttatcta aattgttgct taacgcgact aataatttta atcttgcttt
     8701 tggaccattc aagccgttag aaaaaataaa accttgttgt gcgagttggt aaccaccacc
     8761 atcgtatgcg taagttggac tcacaatacc attaaaggaa cgtgaaacta gcacaatagg
     8821 tatatttaaa gatactaatt gttgaatgcc ttctaatgcg cttggaggta tgttgccttg
     8881 tcctaacgct tcaataacca taccatcgat accttctcga ctataaaaac taaaaatgtc
     8941 acctggcata cccatatatg cttttactaa tggtacattt agtttgtcat tgacattttc
     9001 caatgcttgt tggcgatatg gcatatgatg gaattgcaca cgatcctttg tcaatacacc
     9061 tagcggacca tgatttggac tttgaaatgt gtttgtatta gacgtatgtg ttttggtaac
     9121 attacgcgcc gtatgaattt catcattaaa tacaaccatc acgcctttat gacgggcctt
     9181 ttcatcagag gcaacgcgaa tagcggaaat ataattatat aatccgtcag aaccaatttc
     9241 attagacgag cgcattgcgc cagtaataac aacaggttgc tcgataccta atattaaatc
     9301 aagtaaaaag gcagtttctt ctaacgtatc ggtaccatgc gtgataacga aaccatcata
     9361 atatttattt gttactgctt ctaaaataat atcctttaat tgtttaacat gttggattgt
     9421 catatgaggt gatggtacat taaaaggatt taattcatct atttgtgcat attgatttat
     9481 gacatcttga tgcattgaaa tagggttgat atcatttgtt actactttat tagattggtc
     9541 ttgtgacata ctaatggtgc caccagtatg aataacaagt agatgtttca tatatttcct
     9601 cctatattta atttacctaa ttatgataaa atattattca taaaacgaca aggaagggaa
     9661 atgacgcatg aaagccatta atattgcatt agatggtcca gctgctgccg gaaaaagtac
     9721 aattgcgaaa cgtgtagcca gcgaactatc aatgatttat gtcgatacag gagcaatgta
     9781 tcgtgcatta acatacaaat atttaaaatt aaacaaaact gaggactttg caaaactagt
     9841 tgaccaaaca acattagatt taacttataa agcagataaa ggtcaatgtg tcattttaga
     9901 taacgaagat gtaacagact ttttaagaaa taatgatgtg acgcaacatg tttcatacgt
     9961 tgcatctaaa gagccagtac gttcattcgc cgttaaaaaa caaaaagagt tagctgcaga
    10021 aaaaggtatc gtaatggatg gtcgcgatat cggaactgta gtgctaccag atgcagattt
    10081 aaaagtatat atgattgcat cagttgaaga gcgagcagaa agaagatata aagataatca
    10141 attaagaggt atcgaatcaa attttgaaga tttaaaacgt gatattgaag ctcgtgatca
    10201 atatgacatg aaccgtgaaa tatcaccatt aagaaaagca gatgatgcag tgacattaga
    10261 tacgacaggc aagtcgattg aagaagttac tgacgaaatt ttagcgatgg tgagtcaaat
    10321 taaataatct aataagtcat aactatctat ttgtaatgtg caacgcttaa cgcatataca
    10381 aaatgaatgt gctgataatg atttactcaa attaaaaggt gatttttatt caatgatgaa
    10441 tgaaagttgc ctttttattt ttggtaaaag ttaatgcgtc agtgaattgt gtaagttttt
    10501 caaaaagtaa aaagaaataa taaaggtgaa ttattagaat tccaaaaata attcattaca
    10561 ttcataaagc attttacaaa tggtaagaaa atgagtgtta caaatctaaa tattgcaaaa
    10621 gaagctgatt tagtcacaaa aaatgtccta tgtaataatt cgagaaagat gcactatata
    10681 cggtcttctt actattcaaa tgtaaaagtt gcttatttgc gtggcttttt gttttataaa
    10741 agtataaaat tttactataa tatatcttgt agagaacaat gaaatgacag acttataaaa
    10801 caaaatagta agctatgcta atttttatac aaaaaatatg tataaacata gcaaactgta
    10861 ttactttgat acaaaaatgg ttgtaataaa tatttatcga tatgacgact tgaatatgat
    10921 aaagtgacat atttatgtat atgactattt cgcaaaatgt aatcgaggta gaatttcttg
    10981 acaattctgt cagtttataa gatgttataa atatgtagtg tataaggagg caaacaagat
    11041 gactgaagaa ttcaatgaat caatgattaa cgatattaaa gaaggtgaca aagtcactgg
    11101 cgaggtacaa caagttgaag acaagcaagt tgttgttcat atcaacggtg gtaaatttaa
    11161 tgggattatt cctattagtc aattatctac gcatcatatt gatagcccaa gtgaagttgt
    11221 aaaagagggc gacgaagttg aagcatatgt cactaaagtt gagtttgatg aagaaaatga
    11281 aactggagct tacatcttat ctagaagaca acttgaaact gagaagtctt atagttattt
    11341 acaagaaaaa ttagataata atgaaatcat cgaagcgaaa gtaacagaag tagttaaagg
    11401 tggtttggtt gttgatgtag gacaaagagg ttttgttccg gcttcactaa tttcaacaga
    11461 cttcattgag gatttctctg tgtttgatgg acaaacaatt cgtattaaag ttgaagaatt
    11521 ggatcctgaa aataatagag tcattttaag ccgtaaagca gttgaacaag aagaaaacga
    11581 tgctaaaaaa gatcaattat tacaatcttt aaatgaaggc gatgttattg atggtaaagt
    11641 agcgcgttta actcaatttg gtgcatttat agacattggc ggtgttgatg gtttagtgca
    11701 tgtatctgaa ctttctcacg aacatgttca aacaccagaa gaagtagttt caattggtca
    11761 agatgttaaa gttaaaatta aatctattga tagagataca gaacgtattt cattatcaat
    11821 caaagatacg ttaccaacac ctttcgaaaa tattaaaggt caattccacg aaaatgatgt
    11881 cattgaaggt gtcgtagtaa gattggcaaa ctttggtgca tttgttgaaa ttgcaccagg
    11941 tgtacaagga cttgtacata tttctgaaat tgcacacaaa cacattggta cgccaggtga
    12001 agtgttagaa cctggtcaac aagtaaatgt taaaatatta ggtattgatg aagagaatga
    12061 aagagtatca ctatctatta aagcaacatt accaaacgaa gatgttgttg aaagtgatcc
    12121 ttctacgact aaggcgtact tagaaaacga agaagaagat aatccaacaa ttggcgatat
    12181 gattggtgat aaacttaaaa atcttaaact ataatttaat atttaatagt caactccaca
    12241 tgtttatgat tgcatgtgga gtatttttat gtaacaaaat atactcggaa tgataacgtg
    12301 gacaaattta actaagtgtt taaaaagata aagttttaag tgctgatttt tatcattaca
    12361 gtaataaact cattttgaat acacagtctc atgtgatatt attaaaaaga tataagaaag
    12421 agaggaagtt agcttatgac taaacctata gtagctattg taggtaggcc taatgtaggt
    12481 aaatctacaa tttttaatag aatagttgga gaacgtgttt cgattgtgga agacacgcca
    12541 ggtgtaacac gagatcgtat ttattcttca ggtgaatggt taacacatga tttcaatatt
    12601 attgatacag gtggtattga aattggtgat gcaccattcc aaacacaaat tagagcgcag
    12661 gcagaaatcg ccatagatga agcgtatgtt attattttta tggttaacgt gcgtgaagga
    12721 ttgacacaaa gcgatgaaat ggtcgctcaa attttataca aatctaaaaa accggtcgta
    12781 ttagcggtta acaaagtaga taatatggaa atgcgtacag acgtgtatga tttctattca
    12841 ttaggatttg gtgaaccgta tccgatatca gggtcacatg gtttaggtct tggtgacttg
    12901 ttagatgcag ttgtttctca ttttggtgaa gaggaagaag atccttatga tgaagataca
    12961 attcgactat ccattattgg acgaccaaac gtaggtaaat caagtttagt aaatgctatt
    13021 ttaggtgaag atcgcgttat cgtttctaat gttgcaggga caacgagaga cgctattgat
    13081 acagagtata gttatgatgg acaagattat gttttaatcg atactgctgg tatgcgtaaa
    13141 aaaggaaaag tatatgaatc aactgagaaa tattcagtat taagagcttt aaaagcgatt
    13201 gaacgttcaa atgttgtttt agtggtcata gatgcagaac aaggcatcat tgaacaagat
    13261 aaacgtgttg caggatatgc acatgaacaa ggtaaagcag tcgtgattgt cgtaaataaa
    13321 tgggatactg tggaaaaaga tagtaaaacg atgaagaaat ttgaagatga agtacgtaaa
    13381 gaattccaat ttttagatta tgcacaaatt gcttttgtgt ctgctaaaga acgcacaaga
    13441 ttacgtacat tattccctta catcaatgaa gcaagtgaaa accataaaaa acgtgttcaa
    13501 agttcaactt taaatgaagt tgttactggt gcaatttcca tgaaccctac accaacagac
    13561 aaaggtagac gtttgaatgt cttttatgca acacaagttg ctatagaacc accgacattt
    13621 gttgtatttg ttaatgatgt agaattaatg catttttctt ataaacgcta tttagagaat
    13681 caaatccgtg ccgcttttgg ttttgaaggt acaccaattc atattatagc tcgaaagaga
    13741 aattaacgat tgggggataa caatgactaa aattaccgtt tttggtatgg gaagttttgg
    13801 gacagccctt gccaatgttc ttgcagaaaa tggacatgat gttttgatgt ggggtaaaaa
    13861 tcaagatgct gttgatgaat taaatacatg tcatacaaat aaaaagtatt taaaatacgc
    13921 gaaattagat gttaacatca tcgctacttc agatatgacc aaagcaattc aatttgcaga
    13981 tatttactta atggctttac ctactaaagc aatgcgagaa gttgcttctc aaattaatga
    14041 taagctgacc tctaaaaaga cttttataca tgttgctaaa ggtattgaaa atgggacgtt
    14101 taaacgtgtg tcagaaatga ttgaagattc tatttcacct gaatataatg caggtattgg
    14161 cgtgttgtca gggccaagtc atgcggaaga agttgtagtc aagcaaccaa ctacagttgc
    14221 tgcttcatca aaagataaaa gtgtaagtaa attaacgcaa gatttattta tgaatgatta
    14281 tttgcgtgtg tacacgaatg atgacttgat tggtgttgaa cttggtggtg cattgaaaaa
    14341 tatcatcgca gtagcaagtg gtatcgtagc tggaattggc tacggtgata atgcaaaagc
    14401 tgcattaatg actcgtggct tagcggaaat tagtagatta ggtgaaaagt taggtgccga
    14461 tcctatgaca tttctaggtt taggtggtat cggtgactta atcgttactt gcacatcaac
    14521 acattctcgg aatttcacat taggatataa acttggacaa ggtgaatcaa tggatcaagc
    14581 attatctgaa atgaatatgg ttgttgaagg tatttataca actaaatcag tttatcattt
    14641 agctaaagaa aaaaatgtgg atatgccaat tacaaatgca ttatatagag tattatttga
    14701 aaatatctca gtaaaagaat gcgtaaaaga tttaatggag cgcgataaaa aatctgaata
    14761 aaatgtgtat tattacacat atacggctaa taattacgta aaatgaatag aaatggctta
    14821 aattcaacgt ttttgccaca taaatgattg cgatttatgt aatgcttgtg ttaaagtttt
    14881 accataatga tataaacata tcattatcaa acctcaggag gtgaatgtct aatgaacaaa
    14941 acagatttaa tcaatgcagt tgcagagcaa gctgatttaa ctaaaaaaga agctggttca
    15001 gcagtagatg ctgtattcga atcaatccaa aactcacttg ctaaaggtga aaaagtacaa
    15061 ttaattggtt tcggtaactt tgaggtacgt gaacgtgctg cacgtaaagg tcgtaaccct
    15121 caaactggta aagaaattga tatcccagca agtaaagttc cagcattcaa agctggtaaa
    15181 gcattaaaag atgctgtaaa ataattttac ttaaaaagcc ctgaataagg gctttttatt
    15241 ttgcttttaa tacttacaac ctgtacataa attgtaatgt tcttctaagt ttttaatctt
    15301 tggaacattc attgtttaag cgttcaaaat attacatgac ataatagttt tgataaaaat
    15361 agcaactaca aaatatacat atgaatattg agattaattg ttagcgttga atttacttaa
    15421 aaggtaacca tgtctactat agtattttac gttatttaaa aagatgaata atgtaaatga
    15481 agtaaaggtt attatgagaa ttacaaaagc tacataaatt actgttagtt taaattgaaa
    15541 tttaaaaatg ataagtaatc ttaatgcttc aaaagatttt aatttcttac atataatgtt
    15601 aatatgaata gtgataatat cgtgaggtgt agacatggaa acaactgtta gcaaattgga
    15661 aagacaaata gaagaaagat taaagggcgt atcagaatat gaatccatta atattaatca
    15721 tcggttaggt aagttacttg atttatatga tattccagat gttgccaaag tggcatgcct
    15781 cacaattgac acatcaatga gacaccttga tgacattaca tataatcatt tgtctaaaca
    15841 ttccattttg attggagatt taattagtgc acatttttat acgctactag cagaaatcaa
    15901 tgatttatca tttcaaaatg aaattagtaa agcaattgtt gaaattaatg aattgaaatc
    15961 ttcattacat catcaagctt taaatgatta tgaaatttct caagcaatag taaaaattga
    16021 aacacttttc ccatatatta cgttatctca tttcggtatc aatattgatg agtcggaaat
    16081 atataattat ttatttgaag atatgtctga ttactatcca tcatacttca agaaatataa
    16141 tcaaagtgaa gtaaagcatt acttacacga tatccaaaaa agttatttaa aaagtagagg
    16201 taattaagca tggctgacaa taaagcaaat aaagagcaag tacatcgcgt ttttcaaaat
    16261 atttcaaaaa aatatgatag attaaataat attattagtt ttgagcagca taaagtatgg
    16321 agaaaacgcg tcatgaaaga catgggtgtt agaaaaggga cgaaagcatt agatgtttgt
    16381 tgtggtactg gtgattggac aatcgcatta agcaaagctg taggaccaac aggtgaagtt
    16441 actggtattg actttagtga gaatatgtta gaagtaggaa aagaaaaaac tgcttcaatg
    16501 gaaaatgtta aacttgttca tggtgatgca atggaattgc cgtttgaaga caattctttt
    16561 gattatgtaa caattgggtt tggattaaga aatgtgccag actatttagt cgcgttaaaa
    16621 gaaatgaata gagtacttaa accaggtggt atggtggtat gtcttgaaac gagccaacca
    16681 actttgccag tctttaaaca aatgtatgca ctatacttta aatttgttat gcctattttt
    16741 ggaaaattat ttgcaaaatc aaaagaagaa tatgaatggt tacagcaatc tacgtttaat
    16801 tttcctggaa aagaagagtt gaagcgcatg tttgaagaag caggtttcat aaatgtaaga
    16861 gtacgtagtt ttacaggggg cgttgctgca atgcaccttg gctataaaga aaaagataat
    16921 accaaaggtg attaacgtgg caaagttaaa catgaacaat gaaattaaga aagtggaaca
    16981 acgacttgaa aaggcaataa aaagtaaaga ttctgtatta gaacaggcat cattacactt
    17041 attgtcttct ggtggtaaaa gagtacgccc agcatttgtt attctgagta gccaatttgg
    17101 caaagatgaa caaacgtctg aacaaacgta tcaagttgca gtcgcattag agttaattca
    17161 tatggcaaca cttgttcatg atgacgttat tgataaaagc gacaagcgtc gaggcaagtt
    17221 aaccatatca aagaaatggg atcagacaac tgctatttta actgggaatt ttttattggc
    17281 attaggactt gaacacttaa tggccgttaa agataatcgt gtacatcaat tgatatctga
    17341 atctatcgtt gatgtttgta gaggggaact tttccaattt caagaccaat ttaacagtca
    17401 acagacaatt attaattatt tacgacgtat caatcgcaaa acagcactgt taattcaaat
    17461 atcaactgaa gttggtgcaa ttacttctca atctgataaa gagactgtac gaaaattgaa
    17521 aatgattggt cattatatag gtatgagctt ccaaatcatt gatgatgtat tagacttcac
    17581 aagtaccgaa aagaaattag gtaagccggt cggaagtgat ttgcttaatg gtcatattac
    17641 gttaccgatt ttattagaaa tgcgtaaaaa tccagacttc aaattgaaaa tcgaacagtt
    17701 acgtcgtgat agtgaacgca aagaatttga agaatgtatc caaatcatta gaaaatctga
    17761 cagcatcgat gaggctaagg cagtaagttc gaagtattta agtaaagctt tgaatttgat
    17821 ttctgagtta ccagatggac atccgaaatc actactttta agtttgacga aaaaaatggg
    17881 ttcaagaaac acgtagtatt tatgaaaagt attgaaagcg ctttaccaac ctgttaatat
    17941 ataatagtaa tatacataaa cctattaaac acagggggat acataaagtg gaacgtacat
    18001 ttttaatgat taaaccagat gcagtacaaa gaaatctaat tggtgaagta atttcaagaa
    18061 ttgaaagaaa aggactaaaa cttgtcggtg gtaaattaat gcaagtacca atggaacttg
    18121 ctgaaacaca ttatggtgaa caccaaggta aaccatttta taatgattta atttcattta
    18181 ttacatcagc accagtgttc gcaatggtag ttgaaggtga agatgcagtt aatgtatcta
    18241 gacatattat tggcagcacc aatccttcag aagcttcacc aggatcaatt agaggtgatt
    18301 taggtttaac tgttggtaga aatatcattc acggttcaga ttcattagag tctgctgaac
    18361 gtgaaattaa tctatggttt aatgaaaatg aaattactag ctatgcttca ccacgtgatg
    18421 catggttata tgaataaaat ataaactgta aacctttacg atttatttat aaaggtagaa
    18481 agggttttgt tatgtggtta gtcattatga ttatacataa caaggcccgt tttttatgtt
    18541 gtagtaaatt acttgaaaaa ttttatagtt ttttggtaac acgtattaaa aagagaggaa
    18601 tattctttat caaatgaaac taaacagaga gaaggggttg ttaaaatgaa gaatattatt
    18661 tcgattattt tggggatttt aatgttctta aaattaatgg aattactata tggtgctata
    18721 tttttagata aaccacttaa tcctataaca a
//
LOCUS       DALAGF010000047        18540 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.047, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000047 DALAGF010000000
VERSION     DALAGF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18540
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.047"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(64..234)
                     /locus_tag="O2692_001495"
     CDS             complement(64..234)
                     /locus_tag="O2692_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299128.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(296..394)
                     /locus_tag="O2692_001496"
     CDS             complement(296..394)
                     /locus_tag="O2692_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299129.1"
                     /translation="MIKKKHLSGSLVPDRCFLIISEEVERFMENAY"
     gene            complement(413..1312)
                     /gene="sdaAA"
                     /locus_tag="O2692_001497"
     CDS             complement(413..1312)
                     /gene="sdaAA"
                     /locus_tag="O2692_001497"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCZ4299130.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(1326..2006)
                     /gene="sdaAB"
                     /locus_tag="O2692_001498"
     CDS             complement(1326..2006)
                     /gene="sdaAB"
                     /locus_tag="O2692_001498"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCZ4299131.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(2009..3052)
                     /locus_tag="O2692_001499"
     CDS             complement(2009..3052)
                     /locus_tag="O2692_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCZ4299132.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            3602..3805
                     /locus_tag="O2692_001500"
     CDS             3602..3805
                     /locus_tag="O2692_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299133.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            3988..4923
                     /locus_tag="O2692_001501"
     CDS             3988..4923
                     /locus_tag="O2692_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCZ4299134.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE"
     gene            complement(5005..5973)
                     /locus_tag="O2692_001502"
     CDS             complement(5005..5973)
                     /locus_tag="O2692_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4299135.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVKVDKHFGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(6106..6450)
                     /locus_tag="O2692_001503"
     CDS             complement(6106..6450)
                     /locus_tag="O2692_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCZ4299136.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            complement(6492..6902)
                     /locus_tag="O2692_001504"
     CDS             complement(6492..6902)
                     /locus_tag="O2692_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCZ4299137.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(7282..9348)
                     /locus_tag="O2692_001505"
     CDS             complement(7282..9348)
                     /locus_tag="O2692_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCZ4299138.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(9649..11388)
                     /locus_tag="O2692_001506"
     CDS             complement(9649..11388)
                     /locus_tag="O2692_001506"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCZ4299139.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAIASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(11435..12124)
                     /locus_tag="O2692_001507"
     CDS             complement(11435..12124)
                     /locus_tag="O2692_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCZ4299140.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(12117..12512)
                     /gene="cidA"
                     /locus_tag="O2692_001508"
     CDS             complement(12117..12512)
                     /gene="cidA"
                     /locus_tag="O2692_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HCZ4299141.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(12725..13603)
                     /locus_tag="O2692_001509"
     CDS             complement(12725..13603)
                     /locus_tag="O2692_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ4299142.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            13770..13988
                     /locus_tag="O2692_001510"
     CDS             13770..13988
                     /locus_tag="O2692_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCZ4299143.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            14377..14808
                     /locus_tag="O2692_001511"
     CDS             14377..14808
                     /locus_tag="O2692_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ4299144.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(14946..16223)
                     /locus_tag="O2692_001512"
     CDS             complement(14946..16223)
                     /locus_tag="O2692_001512"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCZ4299145.1"
                     /translation="MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKKEPRANTQAAEHILQEIRQQ"
     gene            16477..17643
                     /locus_tag="O2692_001513"
     CDS             16477..17643
                     /locus_tag="O2692_001513"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCZ4299146.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(17813..18334)
                     /locus_tag="O2692_001514"
     CDS             complement(17813..18334)
                     /locus_tag="O2692_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCZ4299147.1"
                     /translation="MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
BASE COUNT         5762 a         3594 c         2816 g         6368 t
ORIGIN      
        1 ttacattgat ttacattgtc ccttttattt attcttttca aatactatcc ccatagcttt
       61 gatttaacgc tttttctcaa taacaaaacg aatatagtag aacatgaaaa cgataatcat
      121 gctgagcgat aaagatttaa ataatagatt gacccacgtt ccctcagtcg tatatccata
      181 tgtaatcgtt gtgttaatga tgaatgctat aaagatgatt gatagtctta gcatatcatc
      241 actcctttta agttatttta gatatacggg ggcgcttttg caatcactat tttgattagt
      301 atgcattttc cataaatctt tcaacttctt cagagataat taagaagcat ctatctggta
      361 ctaatgatcc agacagatgc ttctttttta tcaatatttt attgttatct cattaattat
      421 ttttaaccat atcttcagct gtgccaaaga ttttacgttt aattgcttcg ccagttggtg
      481 tgcctgctag tccacccaat ccagtttcac gtaatgatgc aggaaggtta cgaccaacct
      541 tatccattgc ttcaataact tcatcaacag ggattctact ttcaatacct gctaatgcta
      601 aatctgctga aattaaagcg ttacccgaac caattgcatt tctcataaca caaggaattt
      661 caacaagtcc ggctactgga tcacaaacta aacctaataa attacttatc gctaatgcca
      721 tagcgtgccc ggatgcttct ggtgatcctc cgaatatagc tactgctgca gctgcggcca
      781 ttgcagatgc tgaaccaact tcagcttggc agccacctgt tgcaccagct acacttgcat
      841 tgtttgctac gacacgccca aacaatgctg aagtgaataa gaaatcaatc atttgctctt
      901 ctgttaaatc atgtgttttt tctaatttaa aaagtgcacc gggaatggta cccgaggaac
      961 cagctgttgg cgttgcacaa ataataccca tcgcagcatt gacttcattt gttgcaatgg
     1021 cacctttgac tgcgtcaatc atttcatatc cagacaaagc atgatgtgtt tcattataat
     1081 cacgtagttt agcagcatca tgaccagtgt agcccgttac actttcaacc ccatcacctg
     1141 tcgtcccttt gattactgcg tctcgcatga catctaaatt ttgtttcatt tgcgctcgca
     1201 cttcatcacg tgatttaccg cttaattcca tttcttcttt aaccatgata tccgcaaatg
     1261 acatattatt ttctacggca taatctatag tctctctaat tgaatcaaac atgtttattc
     1321 cccctctaat ttatatagga aacgtttacg tcactgtatt tctctttaat tgtatttaat
     1381 gttgattctg agattgcttt atttaatggt attacaacca agcatttatc ttcatctatc
     1441 ttaataaatt catctttaca gtctaatttc atatcgttga tatcattgat gaaatgattt
     1501 acttgtgctt tagtcatatt tccgtcaaca actaaaattg gtaatccatg atttaaatct
     1561 acttctagtc catttatatg aataccttta attttaattg taccaccacc gattgaaata
     1621 ccgataattt caatgtagcg accatcatta cgagatgatt tgatataagc acagtttgga
     1681 tgttgaccaa tactatcgcc ttcttcttcg atgatatcta ttttaatacc atcatcagct
     1741 gcaatttcta atgaagattt aattcggtta tcaaatgttg aatatcccat tgctccaccc
     1801 acaatagcga catctgtacc atgtccttgg tgtgtttgag caaatgattc ataataatgt
     1861 atttcaatat ttttaggaat atctcccaat attgcgcgtg ctgaattccc aatctttact
     1921 gcaccagccg tatgagaact tgaagggccc atcataactg gtccgataat atcgaaagca
     1981 ctttgataat catagctctt tgccataatt aaacactctc cttaatatga ttctttttgc
     2041 tcggcatttt aaagttgata ttcattaaat taaacttatt aattagtgtt ccaataatat
     2101 aggctaagac aatgctgaca ataatcactg ttgcgattgt tacaattgat gtcactgcat
     2161 tattaaagcc aaacaatacg atggcgcctg caattggtgt tgccatacct ttgacaccta
     2221 ttactagtcc gctaaatgtc acgatacatg cgttgacgac cccaatcagt gcatttgtac
     2281 catatagttg tactggatat tgcgctatta aatcaatttg cgtcaatggc tcaatacaaa
     2341 ctgcaaatgc ttttgacggt ccaccaatgt ttaattttcg gaataaaata aggttaacaa
     2401 atgagctacc tgtacatgtt agtgctccaa tagccatagg aacacctgtc agtcctaata
     2461 aacttgttaa taccattgaa cttagcggtg tcatacctgt aacaggaatc actagtccta
     2521 aaatgaccgc taatgcatat ggattgttat cacctaccgc agtgacagca ctacctattt
     2581 gttttaatgt tgctagcaca ccaggtgtaa tgattgatgc aagtccgaaa gcaattgctg
     2641 gtgcaaataa gatcaccaca attaagtcca agccttctgg aactttcttt tcaatccatt
     2701 taattaaaaa agctacgcca taagctgcga tgaatgctgg taataattta aagtcatgta
     2761 atactaaacc aacaatgacc gcaaatactg gtgcaacgcc taagtttaag cacgttagaa
     2821 tacctactgc gataccgctt aaacttcctg ctaaatcccc aatatcttgt agaaatttaa
     2881 tatcaaatac gccaccaata gcataactta agaatgcttg tggtagaaat gtcgcacaag
     2941 ctgcaccgga taatgcttgt agtccttgtt taccgtacgg tgcatacttt aaaaatagcg
     3001 tcatgatcac taaaaccaag actaatgtgc ctacacctaa cagaatatcc atttcccaaa
     3061 aacctctctc tatgtttatt ttattttcag accataaaca tcgtacaccc ttaagaaaac
     3121 gttttcaact tttatctgtt atcaaatcaa atatttaagt gaaatatttg atatttgtga
     3181 aagattttta aaatggattg tttcaaaaac tacttatgtt gtcgttaata tttactaatt
     3241 aactttttac tctatatttc aaacagttgt gtgacagttt tttgataact tttttacatc
     3301 tgaaagtaag taatatttct aaaaactttt aatatttata cactttatct ttcgagctat
     3361 ttattttttg tttttgtgct gtgtttataa aaccgcacac tatattcaaa aataacttat
     3421 tacacatata tttgcaataa gataattaaa gtaggatatt atttttagtt ttctgatagg
     3481 aatgatgata gtctataggt tgaatcttta ctttttttaa agctaaattt acatcaactt
     3541 aacaatggtt ggttatactg aagatgaaaa tatttagtat aacttagtgg aggcgataaa
     3601 ggtgcaattg agtcattccg ttaaagttgc aatttctatc tatttagcac ttatctttat
     3661 aacgttcact tcttatttag tcattatttt atatacgagt atgactggac atgatgtatc
     3721 acatttcgtg ttagatagtc agcattctca tcatggatct cttacgcaaa aacatttgag
     3781 tcttcctgaa atctcattta aatagtttat cctctgtgtt tcaacattca tttcccatat
     3841 cgattcattt atctatcatc tagaccacta catcttagat gattttttta ttttctcatt
     3901 tcactctttc ttaaaagtcg atataatgaa ttaaatcatt atcatacacc gacatatttt
     3961 atgttgttgg tgttaagttt taaaggggtg agatacttgg cgaataatca ttcagctttg
     4021 cgtttaaaag gaattatact tgccattgtc ggtgcttgtt tatggggatt aggtggtact
     4081 gtttctgatt tcttgttcaa atataagaat attaatgtcg attggtacgt cactgctcga
     4141 cttgtagtca gtggtgtttt cttacttatt atgtacaaaa tgatgcaacc caaacgttca
     4201 atatttagcg tattccaaga tcgacgtatg ttaggcaaat tacttatctt cagtatactg
     4261 ggcatgttag tagtacaata tgcttatatg gcatctatta atacaggtaa tgctgcgatt
     4321 gcaacattac tacaatacat tgcgccagtt tatattatta tttggtttgt cataagaggc
     4381 gttgcaaaac taacattatt tgatgtgctt gctattatca tgacactatt aggaacattt
     4441 ttattattaa caaatggttc attttctaat ttagtcgtca atcctgcaag tttattctgg
     4501 ggtattttag ctggtgtagc actcgctttt tacacaattt atccttcaga cctacttaac
     4561 cgcttcggtt cgattctaat tgtcgggtgg gcaatgctta tttctggtgt tgcgatgaat
     4621 ttacgccatc caatttggca cattgatatc actaaatggg acatatcaat tatattattt
     4681 ttaatctttg gtattatcgg tggtaccgca ctcgcatttt atttctttat cgacagttta
     4741 caatacatat cagcgaaaga aacaacatta ttcggaactg ttgaacctgt cgtagccgtt
     4801 atcgcaagca gtctatggtt acatgtggca ttcaaaccat ttcaaatcgt aggcatcatt
     4861 cttattatga ttttaatttt attactatca cttaaaagac aacctgaaac attagatgaa
     4921 taagaaaact ctgataatca ctttagcaag taactattat ttaacaacgt agttacctta
     4981 taggtgatat cagagttttt tattttagtt aataatattt ttcacttggt ataaaaagcg
     5041 tcgtcgctct ggtatcggaa atactggata aatatggaat tggtaatatc caggtataaa
     5101 gtcatgttcg atacctttcg cactcaacaa ttgcgataag ttcaaagcat ctgggtacag
     5161 tacttctttt gtaccaacag ttaatgtaat acgacctaaa ccatctaagt caccattaat
     5221 tggtgatact ttgtagttat ctaaaggtgt gtcccctgcc cattgttcag ctaagaacac
     5281 actgccatcc acacctacca ttgggtcttt ctttaagtag tcaggaattt caggatgctg
     5341 catcgttgca tctaaaactg gtgaaattaa tacaatatgt cctggttgca caatatgttt
     5401 ttcttttaac aattgagcaa atgataaagc aatttgaccg cccgcagagt cacccattac
     5461 aacgatttgt ttagaatctg ctacttgatt caataaatca tggtacaact tttcaaaaag
     5521 cacatacgtc gcttgataat cttgatgcgg aatcttcgga tatactggca tgatgacttt
     5581 agcattgagt gtttctgcaa gttcatcaat aaattcgaaa tgaattttga gtgggtcttg
     5641 gaaccatgcg cctccatgtg catataatac aacgcgttga tgcttatctt gtttatcgtt
     5701 aacggtataa acggttgaac cgaagtgctt atccactttt actggcatct taaactgata
     5761 attggttccc tcatattcgc ctgctgtctg tggcgccatt ttttctaatg ctttcatcgc
     5821 ttcttcttta tacataaaac gacgcttagc tctagataat cgtatacctt gttctaacat
     5881 ataacttttc acactgcgtt tttctttaat tctaatatgt gcgtatgctg caactaaaaa
     5941 tccaaacgca agtgtagacc attttttacg catttgtcgt cactcctctt tgatgatttc
     6001 tactatagtt ctactgtacc atgctgattc taattactac caatatttac tgggaaataa
     6061 gttatgatgt ctacataatc ttagcaatgc taatcatttg cgatattaat gctcatcgac
     6121 atcgttattt acactatcat tcatttgctt aagatgatgt tcaaaacgtt gcatatcgat
     6181 aaaacgccct tgacgatgca tttcttttcc attcataaaa attaaatcca caggtacagt
     6241 gaaaatattt aattctccag caatagcttc cacctgactt tgattaatca cagctaatgg
     6301 cacattggga tatgattgca tcaagtcttc aatttgtggt aaaacggcat gacacacgct
     6361 acactgatcg cgcatgacat gtacaactgc taacggatat tgatgaataa attcacggaa
     6421 tgcttcgaca ctatttaatt gcttaaccat ttcgaacacc ttcttacttt ttatatttta
     6481 aaatgaagtc attattttgt tgctaaaatg tcttgaaaca cttgtggcac agaggttatc
     6541 ttcttatcac tcaatctgta aaaaccgcct gtctgtgtac caatacaatg cacgccatgt
     6601 gcggacacaa tttcaaactg aaacacccat ttcatcttgc ttgctttaac taagtaaata
     6661 cgacctactg gtttgtcatg tattgtgaca ggtactttat actctgcttc cgttttcata
     6721 agtataggtg aaatgccttg attgatcatt gttgagtaat tcatgtgctg attaataaac
     6781 gctgttctca aatcttcgaa ccatctaata taaacgatat tactaacgac acccatcgca
     6841 tcaatgtcgt aaccattcac ttcgatttct ttctctgcaa ctaatagacc ttctgttgtc
     6901 ataatgacaa atctccttat cttacaaata gatttaaaat catgttaatt tatttttatt
     6961 actttcatga taaaaataca agcaattgaa ctttattacg gaaaatcaaa cttttcaatg
     7021 agactattaa tttggctagt cgcgaagatg catttttcta aaatgtgaca aaaacataag
     7081 caatgttttc tatatctaac actaatattt aacttattat catcaacaca cctgctatac
     7141 atcattgcgc actaaaaagg cagtagcttc attttaagct actgcctttt gtaacgtatt
     7201 ctctttcaac tcgctacttc gcatacgcac tacgaatgac taaaagttca ttcatttgat
     7261 atgctgtttc atttttaatc attatttgac tgtcataatt agcttacctg gatcagcatc
     7321 ttgtacatct tcaatgacaa gttctttatt ttcaagattt gtaataatca ttggtgtaac
     7381 gatgcttggt gcgtgtgctt tcaagtatgc taaattcact ttcattaatg gttgaccttg
     7441 tgttactttt tcatcaacgt taatcagact ttcgaatcct tcaccattca gtttcactgt
     7501 atcgatacca atatgaataa gtacttcgac gccactttca gattctaatc ctatcgcatg
     7561 tttcgtaggg aagattgttt tcactgtacc atcaaacggt gctacaattt cacctttttc
     7621 agggataaag ccaacaccat cacccatcat tttaccagcg aacacttgat caggtacttc
     7681 tgataatgga atgatttgac cgatacctgg tgcatagatg tctgttgttc caagtgcttc
     7741 tacgtgaact ggttcatctg acatttcttc agtcactgtc gtttcacttg gtttcgtaat
     7801 ttcaccactc ataatcttgg ccatatcatg tttaatttga tctgattttg gaccaaagat
     7861 agcttgcata ttgtttccaa cttctaatac acctgatgcg cctaaagctt taatacctgc
     7921 tacatctact tttgatttat caaccacttc tacgcgtaga cgtgtaatac atgcatctaa
     7981 atgtttaatg ttttcttttc cacccattgc atctaagaca tcaaatggta attttgcgac
     8041 actagagtta cgaatttcag tttcttcatc ttcacgacct ggtgttttca atttaaactt
     8101 acgaattgca aagtcgaata agaagtaata cacgatagca tatacaatac cgactggaat
     8161 aactaataat gcgtgtgaac gatcccagtt taataaacca tataaaatat aatctatgaa
     8221 accacctgag aatgtcatac caattttaac gcctaataaa tgcattacta agaatgatgt
     8281 accagctaat aatacgtgaa ttccataaag tactggtgct acaaataaga atgaaaattc
     8341 taatggctca gtgataccag ttaaaaatgc agttaatcct gctgataaca ttaaaccacc
     8401 cacgactttt ttacgttctg gtcgtgcatt tttataaata gcaaatgccg ccgctggtaa
     8461 accaaacatc ataaatggat atttaccagt agtaaatgca ccagcagtaa atggtacgcc
     8521 atctttcaat tgtgccatcc aaatacgttg gtcaccacga accaattcac ctgcgtgatt
     8581 tgtataactt ccgaattcaa accagaacgg tgaatagaaa atatgatgta aaccaaatgg
     8641 aattaatgag cgttcaataa taccgaatat aaacgttgtt aatgttaaat ttttatttaa
     8701 taagaaattc gataaactat ttaatccatc ttgaattggt ggccacgcaa agcttaaaag
     8761 cacacctgtt gcgattgcta cgaccgatgt cacaatcggt acaaatcgtt tacctgcaaa
     8821 gaatcctaaa aatggtggta gtgtaatatt ataaaatttg ttgtaacacc atgcggctaa
     8881 agcacccatg ataataccac caaacacacc cgtttgtaac gttggaatac ctaatactaa
     8941 agcatgtgct ggttctttcg ctgcttgact taattctttt gcccctttgg catatgagaa
     9001 aatgtcatca attgtaatgt gcaacacttt ccccattgtt gcattcataa ttaagtaacc
     9061 tactagcgct gctaatgctg caacaccgtc tcctcctgct aatccaagtg ctgtaccaac
     9121 tgcaaataat aatggcaagt tatcaaatac aacttgtcct gctgcttcca tgaccgacga
     9181 aatcattaca atgatatcgt tttttaacca tggtgcaatt tctactaatt gttcgttgtg
     9241 cattgcgtta ccaaacgcta ataaaatacc agctgctggt aaaatcgcaa caggtaacat
     9301 taatgcttta ccgatacgtt gcaattgtcc aaacaatttc ttaaacatat aaacatcccc
     9361 tatctcaatt tccgcttcca tgtacaaaaa aagcaggcat gaacaaacaa gactattctc
     9421 atagtgcggt tgtttgctca tgcctgcatt accagtaaca cgcaagtgat atctaattca
     9481 tagctattat acatatttgt aaaagcgttt tcaacaactt tttcacaatt ttatcatcaa
     9541 ttcagtacga tcatttgtga taaataaaca aaacggcaat tcccattttg ttgaaataga
     9601 aattaccgtt tttatagttc aattcaattg tgataacctt taaatcagtt ataagaaacg
     9661 ttttgctgcc actgaaattg gtggaatttg atctaaatct aaatgtttat cttcagtaat
     9721 tgatctaaat gcccacttac cataaccaag cgcttcttca tttacaattt tacctggtaa
     9781 tggcgcagca ttaggatcaa catatacatc tacaatcgtt ggtacatctt gtgctaatgc
     9841 ctcttcgact atagcatcta cttcgctagc actcttaatt gtataacctt taccacctgc
     9901 tgcctcagca aattttgcat gatccatatc agaaaaatca actgcatatt ctaattcacc
     9961 agctgcttgt tgttcatatt taataaatgc taactgtttg ttattaagta caaatacagt
    10021 taaaggtaaa tcatattgta ctgctgtagc gaagtcttgc attaccattt ggaatgcacc
    10081 gtcaccagca attgcgatgg cttgtctatt tggatatgca atttttgatg caattgcacc
    10141 tggaagaccg caacccattg tacctaacca acttgaaatg atgaacttgt tatttacacc
    10201 aaggtttaag tatcgagttg accaaactgt tgctgtacct acatctgctg aaatcactgc
    10261 atcatcttta ataaatttat tgattgatgc cattaatcgt tctggacgta atggtttact
    10321 attattattt ttatcttgtt ccatccattt atcccaaacc gctttacgtt ctaacgtttt
    10381 gtttaagaat ggtctttcag caacatgttt aatattttca gttaactgat gcaacgcaat
    10441 tttactatct ccaacaattc ctacattaat attgaaacga tgtccgatat ttttaggatt
    10501 tgtgtcaatt tgaatggctt taatattttt cttaggtaag taatccacat atggatagtt
    10561 tgtaccaacc ataattaata aatccgcttc ctgcattgtt tgataagatg ttttggtacc
    10621 gattttacct aagttaccga tactatatgg atgatcatcc ggcaagattg ttttagctgg
    10681 taatgaatga atgacaggaa ttttagccat ttcaataaat tcacgtagct catctttcgc
    10741 atgtttcgca cctacaccaa ttaacatgac aggcttttta cttttattaa ttagtttaac
    10801 cgcttttttg atgtctttat attttggtga tactactgtt ggtcttgatg tatctactgg
    10861 tttattcgtt gtatctttaa ttttttcagt taataagtcg ttaggacaaa taacaacagc
    10921 tacacctttt tgttcatatg ccgtacgaat tgcttcgtta acgatttcaa acacattgtc
    10981 acctttttca atttggtgat tataaacggc tacatcttca cataattttt gtaaatttgt
    11041 ttcttggaat gctttcgttc caagtgctgt actattcgtt tgtccagata atattaattg
    11101 cggtacatta tccattttgg catcatacat accatttaat aaatgaatta aaccagggcc
    11161 accgatactt aatgccacac cgattttacc agttaatttt gtgtaaccag cagccgctaa
    11221 gcttgctact tcttcatgac gtacatgata aaatttaaat tgatctctca ctgtacgtaa
    11281 actatcgact actgcgtcga ttgagtctcc tggaatacca tacaagtgat ctatatccca
    11341 tgcttgtaat gctttaacta atgcttcatt tgcttttatt tttgccatta ttactaatag
    11401 cctccctttc tgtctatcat tttaaatatc taaattaata gaataataaa attagaacag
    11461 gtattaacac tgaactaata actgcagtta aaatcatccc aattgaacta aatgcaccgg
    11521 attcaatatc catttctagt gctttagctg tgccaaatgc atgtgacgca ttcccatacg
    11581 ttaatccttt ggcgatagaa gattcaaatc ttccaaatct taataacatc gaacctaaaa
    11641 tactaccgat taaacccgtt gtgataataa aaagtacggt catcgtatct gtaccaccta
    11701 gttcatgtga cacttcgata cctactgcgg ctgttataga tcggggcaat aacgttacaa
    11761 tgacgtcttt agaatagcca aatgctttaa gtgttaagaa cactaacatg aaattcagca
    11821 tgacgccagt taatacactt gcaaaaatga tagagacatt gtctttaatt ttctctctat
    11881 ttttataaag tgggtacgct aaacatacga ccgttgcgtt taaaatatgg ttgatccaac
    11941 tgccaccttt catatacccg ttataactaa ttccaaagat aagtaagaca aaaataattc
    12001 ctaaagatgc aattaatgct ggattcaaaa atgggttcgg atatttttgt tgtaaccttt
    12061 tagcgaaata atataaaacg acagtcaaca aaatcattaa taaggcttgc acgtaatcat
    12121 tcataagcgt ctacaccttt acgatgttta tgtttaacag acattttttc agcaatataa
    12181 cctgaagata atgcaacgat acatgttcct atgataatga ctgcgaaaaa gagtatataa
    12241 tttagcgtaa tttcggaagc aacatccata atacctacaa ctgacggtat gaagaaaaag
    12301 accatcgtct ttaataaaaa gtttgcaccg tcttctaccc aagttagcgg tacaatctta
    12361 aattgtaata gtaaataaaa taaaaataga ccaacaatac tgccggctaa gggaagatga
    12421 aaaatctttt gaatttctgt gccaatataa gtaatcacaa tgatgattcc tagttgtagt
    12481 agtagtttga ttattaattg gactttgtgc atggcgccat ccctttctaa atatgtctaa
    12541 attgttacaa taactattat aaagatggcg tacacaatat aaaaatactc tttttttata
    12601 gtttgtatta ctattttcta tggaaacgct ctctaaattt cacttcattt tcacaaaaaa
    12661 acactcatta tttcaatgac ccacgtaatg atgcgcctta aataatgagt gctttattaa
    12721 tatgttagcc taaacggtct ttcaaaaatt ctatccattt cgttgtggca tgacttaatt
    12781 gtttatcctt cttccaaaca acacctaatt cccaatgtac atgagcatct tcaatgcgta
    12841 gcagcttcac atctccattt agttgctctg aaattgatgt tggtaaaata ctaataccta
    12901 attcattcgt aactaaatct tctataacat gccattgtga aatttgcgct acagtattcg
    12961 gaacaaagcc aacgttttta gcattttcaa taattttatc attcaagtaa aagtctttat
    13021 taaataaaat gaagtcttca cctgctaaat cttcgagttt aacagtttca tattttgcga
    13081 gtcgatgctc tctgctcacg ataagtcgca aatcttcctt atctagggta gtataatcga
    13141 aaatatgatg atcgactggc aaagtggtca cgcctatatc tacttcatca ttgataattt
    13201 gctgttcaat tgttttaccg ccattttcta tcaaattata tgtaacattt ggatattgtt
    13261 gatggaatgc accaagaata ttgataagaa tcttcatatt catgactgcc gacatgccca
    13321 tgtttatatg tcctgtttcc agtccattca agcgttccat ttcagatggt aaataatcat
    13381 acagtgcaac aatttcttta cttttctcat aaaaaatttg acctgcatcg gtaagaatta
    13441 aatgtctttt acttctatca aataagggcg tgcccatttc attttcaata tctttaattg
    13501 ctttactaat tgtaggttgt gcaatatata atgatttgga agcattagtc atgcctcctt
    13561 gcttaacgac ttcaataaaa tatttcatat gtttgatatc cactaacatc atcctaacta
    13621 tttcaacacg tttatttcat tatacacaac ttcttaaaca ttcaataaat ttagatttat
    13681 aaaattgctt tataaaaagt tttatgtaaa atacacattc ttttatggta ttatattaat
    13741 ggctcaagta tattaatcga ggtgaggtat tgagctttta cgaatttatg caaagttttc
    13801 ttggtgatga cacaccatta ggtgaattgg ttgattggat taatcaagat agcaatttcc
    13861 ctagagaagt gaagagccat agtgaaatat tgtcatattt tcgaacaaat ccatgtcctg
    13921 aaagcatctc agtacctgta attaaacggg cactatcagt tttcaaccaa ttcacaaatg
    13981 tacaatgata catgaataca tggagtcaca tcatctcatg ttagcgctct gattgcaggg
    14041 cgctattttt aatgttattg ctacatttag tatattgtga ttgctatttt ctttcagtta
    14101 tagaaccatg tacctttttt ttaaatcatt ttatattttc ccactaatat ccgctgttaa
    14161 tcaatcctga catccttgta tcactatgac aattaattgt taaatacatg aatttctaca
    14221 ttttatgaaa aaatccattt ttattacaat tcaacacttt atatgacaac ttcattacag
    14281 ttacttttat tgttgattgc ttacattgtt ttctaaaaaa aatttgttat cataattaac
    14341 gttgaataaa gaaaaaaatt aacttgggag ataaaaatgg aatataaaaa gatactaatt
    14401 cgtttattaa ttgcttttgc agtacttttc tcagcagatt tcacttatca atccgtcgaa
    14461 caaacgcatc aatcgcatgc cgcagttaat tattatagta aaaaccaatg tacatggtgg
    14521 gcatttaaac gtcgcgcaca agtcggtaaa cctgtttcta atagatgggg caatgctaaa
    14581 aattggtatt acaatgcacg taaatcaaaa tatgcgactg gtcgtacacc aagaaaattt
    14641 gctgtcatgc aatcaactgc aggatattat ggacatgtcg cagttgttga acaagtatat
    14701 aaaaacggta gtattaaagt ttcagaatac aacttttatc gcccattaaa atacaataca
    14761 cgtgtactaa gcaaaaaggc agcacgtaac tttaactata tttactaatc aaaaaacttc
    14821 tatcacgaac gcttcaattt cctgtatgcg tgtgatagaa gtttttattt ttgttcatat
    14881 taatttatct aagcgctacg atggactgac ttaaactttt ctttaaaatc gctattcgcc
    14941 atttactatt gttgtctaat ttcttgtaaa atatgttccg ctgcttgtgt atttgcacgg
    15001 ggttcttttt tcaaagcttc agctacttta gcaatttcat caccttttgc ccctacaacg
    15061 atagctaatg atttatattg taaactcata tgaccttgtt gaataccttc tgacacaagc
    15121 gcgcgacatg ctgcaaagtt ttgcgctaaa ccaacggcag caactacatg acctaattct
    15181 tgtgctgact ctacatttag tagctctaat gaagctttag caattggtaa tacttttgta
    15241 ccaccgccaa cgattgccaa tgtcataggc acttcaattg taccaatcaa tcgttgacga
    15301 tcttgatcgt aacgccatgt agcaatacca cgatactgtc cgtcacgact cgcgtatgca
    15361 tgcgcacttg cttctgcacc acgcgtatca tttcctgttg ctaaaacaac agcatgtatg
    15421 ccattcataa cacctttatt atgtgttgct gcacgatgaa tatctacttg ggccaataca
    15481 gaagcacgtt ccattcgttt ggcaacctct tctccagttc tctcgcccct tgctaaatct
    15541 ttaacatcaa tttcgccttg aactttaaca acggacgctg ttgcatgatt ggataaaata
    15601 ctcattaaaa tgtcgctttg cggaaattca ttttttaaaa atgcagttat ggcctctaaa
    15661 atcgtattaa gcatattagc gcccatagca tctttcgtat caacaaatac ttttaaagat
    15721 agtaactgtt gctcaggaaa tgtatcaatc gctatacgtt ggtaaccacc accacgcgct
    15781 ttaatagaag gatatgcctc atccgcaatt ttatgaattt gcttttctaa agctttaatg
    15841 tctgctgata atttttcagt atcgtcaacg ccatcaaaga cgatttgacc tatcataata
    15901 cgttcagaag ataccgtttt aaatccgcca gtctgattca ctagctttgc accataacta
    15961 gctgcagcga caactgaagg ctcttccacc atcataggta caacatatgc cttatcgtcc
    16021 acaatgatat tcggtaataa tccaacgggt aatgcacctt gcgcgatgac attttcaatt
    16081 aaactattgg ctacttcttc atcgattaat ggatgattca gtaaaatgtc gaattgttct
    16141 tctgataacc attgcttatc aaccaattgt tgtaactttt ctttacgaga taaatgtcga
    16201 aaattcttat ctaaattttg catggacgta ctccttttac ttcacataat ttttaacatt
    16261 tcaatcacta ctatttttac cacaaaataa cgtcattcgt cttaaaattc aattgaataa
    16321 ttgtcgtttt gactttaaaa taaaacaagg taaattaaaa cgcttacaag aaacgacaaa
    16381 tcatttttaa atttagtata tttctttgta taaaattagc atattctgat atgatacaag
    16441 tgttgctttt ataaatttga aaggatgtaa aaccttatga caataggtat cgataaaata
    16501 aacttttacg ttccaaagta ctatgtagac atggctaaat tagcagaagc acgccaagta
    16561 gacccaaaca aatttttaat tggaattggt caaactgaaa tggctgttag tcctgtaaac
    16621 caagacatcg tttcaatggg cgctaacgct gctaaggaca ttataacaga cgaagacaaa
    16681 aagaaaattg gtatggtaat tgtggcaact gaatcagcag ttgatgctgc taaagcagcc
    16741 gctgttcaaa ttcacaactt attaggtatt caaccttttg cacgctgctt tgaaatgaaa
    16801 gaagcttgtt atgctgcaac accagcaatt caattagcta aagattattt agcaactaga
    16861 ccgaatgaaa aagtattagt tattgctaca gatacagcac gttatggatt gaattcaggc
    16921 ggcgagccaa cacaaggtgc tggcgcagtt gcgatggtta ttgcacataa tccaagcatt
    16981 ttggcattaa atgaagatgc tgttgcttac actgaagacg tttatgattt ctggcgtcca
    17041 actggacata aatatccatt agttgatggt gcattatcta aagatgctta tatccgctca
    17101 ttccaacaaa gctggaatga atacgcaaaa cgtcaaggta agtcgctagc tgacttcgca
    17161 tctctatgct tccatgttcc atttacaaaa atgggtaaaa aggcattaga gtcaatcatt
    17221 gataacgctg atgaaacaac tcaagagcgt ttacgttcag gatatgaaga tgctgtagat
    17281 tataaccgtt atgtcggtaa tatttatact ggatcattat atttaagcct aatatcatta
    17341 cttgaaaatc gagatttaca agctggtgaa acaatcggtt tattcagtta tggctcaggt
    17401 tcagttggtg aattttatag tgcgacatta gttgaaggct acaaagatca tttagatcaa
    17461 gctgcacata aagcattatt aaataaccgt actgaagtat ctgttgatgc atatgaaaca
    17521 ttcttcaaac gttttgatga cgttgaattt gacgaagaac aagatgctgt tcatgaagat
    17581 cgtcatattt tctacttatc aaatattgaa aataacgttc gtgaatatca cagaccagag
    17641 tagtcggtgt atttaaaaca catataataa aacctaaaag cagcagtaag accacttcta
    17701 attgaaatcg tcttactgct gttctctatt tataacactt cgtattgaat gaattcatta
    17761 tgcctatttg acacattatt gaagttttcc taatgcctgg atcctttata cgttacggct
    17821 tcgtgctatg ttttggtaca taaagctttg acatatcgat attctccaac tctaacagct
    17881 taattttatt attaatcgtt ccaccgaacc ctgttaagct acccgtttta ccgacaacac
    17941 gatgacatgg cacgataata gataatggat tacttccgac tgcacctcca accgcttggg
    18001 ctgacatttt tggcttgtta agcagcttgc ctactttttt ggcaatagca ccatacgttg
    18061 ttagagtccc ataaggaacc tgtcttaatt cattccaaac acactgttga aaatgactac
    18121 ctgttggctt taaaggtatt gtgatttcag gattgtcacc tttaaaatac gcgtctaacc
    18181 actgtgtcgc ctctctaaat atcgctaaag acgtattttc ttccctagta ccatcacctt
    18241 gttgattttc aaacaaaaca gcggtcagac ttaccccatc actcaaaagt tccaatcgtc
    18301 ctacaggcga atcatagtaa ctcttatact ccataaaaat cccccctttt tctaatcata
    18361 cgccttaatt gttaaattag aaaatattca tgttcacatt ttttaaaaat aaataagttg
    18421 tcaattacgt atataaacac gtaataccag ctatcacttt gctgcaatat acagttacat
    18481 atcttactac acgtgctaac ctcttacttt gtaaaccaaa tcttaaatta aaatattgaa
//
LOCUS       DALAGF010000048        18389 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.048, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000048 DALAGF010000000
VERSION     DALAGF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18389
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.048"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            41..1441
                     /locus_tag="O2692_001515"
     CDS             41..1441
                     /locus_tag="O2692_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4299148.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLITVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            1667..2602
                     /locus_tag="O2692_001516"
     CDS             1667..2602
                     /locus_tag="O2692_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCZ4299149.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            2924..3775
                     /locus_tag="O2692_001517"
     CDS             2924..3775
                     /locus_tag="O2692_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCZ4299150.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYQTWK
                     DGMNPIK"
     gene            complement(3867..5363)
                     /locus_tag="O2692_001518"
     CDS             complement(3867..5363)
                     /locus_tag="O2692_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCZ4299151.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDS
                     VTSKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQTNYKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLSLCICIALALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
     gene            5595..7424
                     /locus_tag="O2692_001519"
     CDS             5595..7424
                     /locus_tag="O2692_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCZ4299152.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSAKNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     LPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(7564..9642)
                     /locus_tag="O2692_001520"
     CDS             complement(7564..9642)
                     /locus_tag="O2692_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCZ4299153.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(9780..11189)
                     /locus_tag="O2692_001521"
     CDS             complement(9780..11189)
                     /locus_tag="O2692_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ4299154.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
     gene            11475..12281
                     /locus_tag="O2692_001522"
     CDS             11475..12281
                     /locus_tag="O2692_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCZ4299155.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLDELQKGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVIDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(12344..13000)
                     /locus_tag="O2692_001523"
     CDS             complement(12344..13000)
                     /locus_tag="O2692_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCZ4299156.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            13113..13586
                     /locus_tag="O2692_001524"
     CDS             13113..13586
                     /locus_tag="O2692_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCZ4299157.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(13709..14920)
                     /locus_tag="O2692_001525"
     CDS             complement(13709..14920)
                     /locus_tag="O2692_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCZ4299158.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(15102..15761)
                     /locus_tag="O2692_001526"
     CDS             complement(15102..15761)
                     /locus_tag="O2692_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299159.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            complement(15821..16963)
                     /locus_tag="O2692_001527"
     CDS             complement(15821..16963)
                     /locus_tag="O2692_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCZ4299160.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            17231..17617
                     /gene="gtxA"
                     /locus_tag="O2692_001528"
     CDS             17231..17617
                     /gene="gtxA"
                     /locus_tag="O2692_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HCZ4299161.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLIITIPITFILSKWILKDSN"
     gene            17751..17858
                     /locus_tag="O2692_001529"
     CDS             17751..17858
                     /locus_tag="O2692_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCZ4299162.1"
                     /translation="MFNLLINIMTSAISGCLVAFFAHWLRTRNNKKGDK"
BASE COUNT         6086 a         2749 c         3403 g         6151 t
ORIGIN      
        1 aaaatttgtt tttcttaaac ataggaggct tatctaattc atggacacat caaaacaatt
       61 tagaggtgac aaccgattgc ttttgggtat cgttttaggg gttattacct tttggctatt
      121 cgcgcagtca cttgttaatc ttgttgtccc attacaatca acatatagta gtgacgttgg
      181 aacgataaat atcgctgtta gcttatctgc cttatttgct ggtttgttta tcgtaggtgc
      241 tggtgatgtt gctgataaat ttggtcgcgt caaaattact tatgtaggat tgatattaaa
      301 tgttgtaggt tcattactca tcatcattac acctttgcca gcatttttaa ttataggtag
      361 aataattcaa ggtttgtctg cagcatgtat tatgccatca acacttgcta ttattaacga
      421 atattatatt ggtacaagaa gacaacgtgc cttaagctat tggtctattg gttcttgggg
      481 tggtagtggt atttgtacgt tgtttggtgg cttaatggct acatatatag gttggcgttc
      541 aatatttgtt gtttcaattc tattaacatt attagcaatg tacttaatca aacatgcacc
      601 tgagactaaa gcagaaccaa tcaaaggtat gaaagcagaa gctaaaaagt ttgacgttat
      661 tggtttagtc attttagtag tgacgatgtt aagtttaaat gtaatcatca cacagacgtc
      721 tcattttggt ttagtttcac cgttaattct aggtttaatt gttgtgttta tctgttcatt
      781 agttggtttt gtatattacg aaaataaaat aaagcatcca cttgttgatt tttcaatttt
      841 taaaaataga ggatacagtg gtgcaactat ttcaaacttc ttattaaatg gtgtagcagg
      901 tggtgcactt atcgttatta acacgtatta tcaacaacaa ttaggattta attcttcgca
      961 aacgggttat atttcattaa cgtatttaat aacagtgttg tcaatgattc gtgtaggtga
     1021 aaagatttta tctcaacatg gtccgaagcg cccactatta ctaggaagtg gctttacagt
     1081 gattgggtta atcttattgt cgttaacatt tttaccagaa gtgtggtata tcatatctag
     1141 tatagttgga tatttattgt ttggtactgg tttaggatta tatgctacac catcaactga
     1201 tacagcagtt gctagtgcgc cagatgataa gtcgggtgtt gcttcaggtg tgtataaaat
     1261 ggcgtcatca ttaggaaatg catttggagt agcagtatct ggtacggttt atactgtgtt
     1321 agcagctaat ttaaatttga acttaggtgg tttcacaggt atgatgttta atgccttgct
     1381 agcaattgtt gcatttttag tcattttact attagttcct aaaaatcaaa cgaatttgta
     1441 aaactgaaat gaaagcaagt tattatgtag ggattttaaa ggaaattttg tgaaagtaag
     1501 tttatcatac acacttaatg ttgcgtattg acgtttaatg ttaggtgtgt tcttttatag
     1561 acgataaaag ctgtgtgcat attaagcgaa tgattttcaa attgacgcta atatgcgaaa
     1621 gtagtatttt taaaatgaac aacaacgatg aagaggggtt tataggatga aaattgcaat
     1681 tgctggatcg ggtgcattag gtagtggctt tggtgccaaa ctatttcaag caggatatga
     1741 tgtcacactt attgacggat atacatctca tgttgaagcg gttaagcaac atggattaaa
     1801 tataacgatt aatggagagg cattcgagtt aaacattccg atgtatcatt ttaatgatca
     1861 accggacgaa agcatttacg atgttgtctt tctatttcca aagtctatgc aattaaaaga
     1921 agtgatggaa gatatgaagc cacatattga taatgaaacg atcgtcgtat gtacgatgaa
     1981 tggtctgaag catgaagaag tcattgcgca gtatgttgct caatcacaaa ttgtcagagg
     2041 tgttacgact tggacggcag gtcttgaaag ccctggacac agtcatttac ttggtagtgg
     2101 accagttgaa ataggtgaac tagtggatga aggtaaagaa aatgttataa aagttgctga
     2161 tttacttaac gaagcggaat tgaatggtgt cattagtaaa gatttatacc aatcgatttg
     2221 gaaaaagatt tgtgttaatg gtacggcaaa tgcattaagc acagtgttgg agtgtaatat
     2281 ggcatcgctg aatgaaagta gttatgcgaa gtgtttgatt tataaattaa cgcaagaaat
     2341 agtgcatgta gcgacgattg ataatgttca tttaaatgtt gatgaagtat ttgaatattt
     2401 agttgattta aatgaaaaag ttggtgcgca ttatccatcc atgtatcaag atttaattgt
     2461 taataataga aaaactgaaa ttgattatat taatggcgca gttgcaacat taggtaaaca
     2521 acgtcatatt gaagcgccag tcaatcgctt tattactgat ttaattcata ctaaagaaag
     2581 tcagcgacac gcacaggatt aattggtgtt ttaggtatga ttacaaggaa atgacaatca
     2641 cgtgatatta cggtcattat taagattgaa atgtaataaa taaagaacag cagtaaggta
     2701 ctttcaaatt gaaatgatct tggtgctgtt tttcttgatt gatcttcgtc ataattcaga
     2761 tttgtcatag gtctacgaca tactattagt atttactaga cagtttttac gacgacactt
     2821 tgaaaaattt tgaggcaaat catttggaag tctcacgtga attttgtaaa ctcatcaagc
     2881 aagtaattat attaaaaaga caaatagaga aaaggtgttt ataatgagta aaatttttgt
     2941 aactggtgca acgggcctta ttggcattaa attagttcaa agactaaaag aagaggggca
     3001 tgaggttgct ggttttacta catctgagaa tggtcaacaa aagctagctg ctgttaatgt
     3061 aaaagcatat attggtgata tattaaaagc tgatactatt gatcaagcgt tagcagattt
     3121 taaaccagaa atcattatca atcaaattac ggatttaaaa aatgttgata tggcagcaaa
     3181 tacgaaagta cgtattgaag gttctaaaaa cctaattgat gcggcgaaaa agcatgacgt
     3241 taagaaagta attgcccaaa gtattgcctt tatgtatgaa cctggcgaag gattagcaaa
     3301 tgaggaaact tcacttgatt ttaactcaac tggcgataga aaagtaacgg ttgatggtgt
     3361 ggttggttta gaagaagaaa cggctcgtat ggatgaatac gttgttttac gttttggctg
     3421 gttatatggc ccaggtactt ggtacggaaa agatggcatg atttataatc aatttatgga
     3481 tggtcaagtg acactttcag atggcgtaac atcatttgtg catcttgatg atgcagttga
     3541 aacatctatt caagctattc attttgaaaa tggtatctat aatgtagcag atgatgcacc
     3601 tgttaaaggt tctgaatttg cagaatggta taaagaacaa cttggtgttg aaccaaatat
     3661 tgatattcaa cctgcgcaac catttgaacg tggcgtaagc aatgagaaat ttaaagcgca
     3721 aggtggtact ctgatttatc aaacttggaa agatggcatg aatccaatta aataataatt
     3781 tatccgttta atatacaaag aataaagact tggtcgaatc gtggatgata tattatcaaa
     3841 cgcacggctc gaacaagtct tttttattat gtcttcgtta tctttgtatg aaggaataac
     3901 agaattacaa ttaatgtact gaataatgca attaatgttg tgattagtgc taatttaatt
     3961 tctattggta gccaagtcag tacaaaagac caattattgc taccgagaat gagatatggt
     4021 aatgcatata atatgagcgc taaagcgata catatacata atgataacca actcaataca
     4081 gcaatccgtt ttgatctacg caaaagaatt tgaccatggc gcatttgcca agcacgataa
     4141 gctgagaata taaatgctaa taaaataagt gttgtcatca aaacggtaac aatattaaat
     4201 tgatctttat attgattgag catagacgca accgtcgaat atcgcttgtg atttacaatt
     4261 tgtgtattaa gatgctcaac taatttgggt acatattccg aatttagatt tgcaagtaca
     4321 acaattccat aattttgttt tggatttagt aaaataaatg atgaaaagtt atctagcgtt
     4381 cctgaatgaa acactaaatg ttcatcatta ttggtaaacc agccggaagc atatgcattg
     4441 gcattaggtt caccaattgt tgaagataaa tttttatgtg attgttgaac taatgatttg
     4501 tatttatcag gtggattaag ttggaatttt atccaatgtt ccaaatcttc agttgatgtc
     4561 atcatatatg ctgatggtgt atcccaaagg ttaaattcag gtttagagac gacaggtgtc
     4621 gaaccttgta attcatagcc aatagcatca tgttttgatt tgtaattggt ttgtttgaat
     4681 gatgtatgtg tcatatgcaa aggcttgagc catgaatttg taatatattt tgtataggat
     4741 tgcttcgtaa cgttttggat aattaaacct aataaatcat agttcatatt tgagtattca
     4801 aattcttctc cgggcttatg atgtaattca tcacccataa ttgcacgggt tacatcattt
     4861 aaacgattat ttttgcttgt cacagaatct tcgcttgtaa tatcactagg tataccactt
     4921 gtttgagcca aaagttgctt aatcgtaata gtttcatttt gaccattata gttcatttta
     4981 aaatgaggca catgtttgga tacggcatca tttaagttta atcgaccttc ttgagctaat
     5041 tttaaaattg caagacctgt gaaagctttc gtattagaag caatttcata ctttgttgtg
     5101 ggagaagctt tgactttttt atcaacatct tgataaccat aacctttatt taaaaaaact
     5161 ttgccatttt ttactattaa aattgatgct ccaggaatgt gtcccttttg taaatcatgc
     5221 tcgataattg tatctatttg ttgttgcgaa tcattggtta accgtgtctt cgtattgcta
     5281 tttaatgtta tatatatagc aattgaaatg gcgactaaaa tgataataga aatggataga
     5341 aaatacagtt ttttagtagt cataaacgtt cctttttata tgttaaattt acttaagtgc
     5401 ctaatgagat ttattttatt ataccagttc tattgaataa attaattaaa gaggtatatt
     5461 taccattatt tttgacacct tatatgttaa taggtgtata attacataaa aaatattaat
     5521 taactctcta tactttttgg aaagataggc gtatagtgat atccatcaaa aactataaaa
     5581 aggaatagga gattatgttc aatcaattta aaagacttat tatagggcaa cctaaaaaaa
     5641 acagagaact aaaagacgaa aagatatcga agtttaaagg gttagcgatt ctttcgtcgg
     5701 atgcattatc ttcagtggct tacggtcccg aacaaatact gattacacta tcagtagtgg
     5761 gtgcagttgc aacttggtat actttaccga ttgcaggtgc tgttctgatc ttattagctg
     5821 ctttaattat gtcttatagg caaattattt atgcatatcc taaaggtggc ggcgcgtata
     5881 tggtgtccaa gaccaattta ggagaaaaat ggggattgct tgcaggaggc tcattattag
     5941 tcgattacat actgactgtc gctgttagta tatcatctgg tgctgatgca tttgttgctg
     6001 cattcccaag tttatatggg cataaagtac tgattgcatg tttacttgta ctttttattt
     6061 taatattaaa tttacgtggt ttaactgaat ctgccaccgt attatcctat ccagtatatc
     6121 ttttcattat agggttagtg atattaatat ttataggtac tttccgtgtg gcgacaggag
     6181 atattcaacc acatatgcat gcatcagtag gaactgcggt tcctggagta acattattct
     6241 tgctattaaa agcattttca tcaggggctt catcgttaac gggtgttgaa gctatttcaa
     6301 atgcggtaac caattttaga gagccaagtg ctaaaaatgc tgtgaaaacg ttaattgcta
     6361 tgggttcaat tttagcgttt ttattagttg gtatcgttgg tttagcatat gtttatggaa
     6421 ttttgccaca aacggaaacg acagttttat cacaattagc tatgcaaata tttggtgata
     6481 atgctgcctt ctattttgta caggcaacga ccgtgatgat tttggtatta gcagccaaca
     6541 ctggatttac agcttttccg atgttagcgg cttcaatgtc taaagataaa tatatgccta
     6601 gaatgtttac tgttagaggt gatcgcttag gctactctaa ctcgattatt attttaggtg
     6661 tattagccat tattttaatt attgtatttg atggtatgac tgaagattta attccattgt
     6721 atgcggttgg tgtatttatt ccatttacac tagcacaatt cggtatggtt attaaatgga
     6781 ttcatgaacg tcctaagaat tggttaagta agttgtctgt gaacttactt ggtggtatcg
     6841 tgacatttat tgtttttatg attttactca taactaaatt tagccaagta tggccgatac
     6901 ttattttctt gccgttcgtg gtgattttct tcttgaaaat taacaaacat tatcgagata
     6961 tcgcagaaca attacgttct gacattgatg ttcttaatgt ggacgtggta gatcgtaatt
     7021 tagcaattgt gccgataaca agtattacaa cagcagttga taagtctatt tattacgcac
     7081 aaatgcttgc gaataatgat gtcatagcag tacatgtatc atttggagat gaagatgaaa
     7141 aagcattcca agagaaatgg aaacgtcatt tcccagatgt aagattagtt attttacatt
     7201 cggaatatcg cagtattata cgaccaattt cacgttttat cgataagatt aatcgtaaag
     7261 ccaacgatca aaactatatg attacagtag tgataccaga atttattact aagaaacgtt
     7321 ggcataattt attacacaat caaactagtt tgcgtatgaa attgtattta atttatcaaa
     7381 agaatgttaa cgtatgtaca attccattta aacttaaaaa atagattcta taatcaaatc
     7441 ggattgatga aaggttattt acttttcatc aatctgattt tttgatgttg aatataaaaa
     7501 ccccgtacat atgagatgat aatgactttg tctcatatgt acgggggtat tgttatgtct
     7561 tgcttaatgt ttggactgta aagacaagac aatttcgttg taattgatat tttctcgaat
     7621 ttgagctgca acgatattgt tgattttatt tttgcgaatt aattcatcta aaagatgacg
     7681 ttgtaagtac aatgcttcca atttgtggtt atggaacata gcttgcggta tgatttgatt
     7741 gctgttttct tgacgttctt ttcgacgttg tgcacgccac tttaatgagc gactcatatc
     7801 atagtaatga ttaatgacta agctaacctc aagtacattt gtactatttt gttcttcttt
     7861 taagcgtaaa ataatattgt gattcacaat acgcataatt ttttgaacat ctttgaatga
     7921 attttcaaat tcttgcttct tagattcttt atttaaggct ttgtagtgct tcatcgcttt
     7981 aagtctaagc cataacatct tgaagaagtt tgcaaatttt tcaacaaatg atgcagtacg
     8041 gtgtgtctct gttaattcga caatattgcg gtaattatta atatcctgat atgttgcctt
     8101 acctttatca attaaacgct caagtgtaga tgtttcgatt acttttgcaa tatcttctaa
     8161 acgtttgagt tcttttgtat tttgattatc aggttctgaa tttaataaaa ataacaattc
     8221 tccatagtat tggtttaata ctgggcgata atttgtatcg tttttgtctt ttttactttc
     8281 gtttttaaaa tgctggatca ctttttgaac aatgaaaatt ttggcagatt gataagtcat
     8341 acctttaaaa gtagtatctt cggcagatgg tgtaattaaa ggtaaaacaa tttgcgctaa
     8401 gattaaacta attaatacca tgaaagatgc aataaacaat aaatcattac ggtattcgaa
     8461 tgcttgtcct tttgtaatga taaatggtaa tgtaagtgcc attgaaagtg aaattgtacc
     8521 gtgaatacca cacatggtca taataaatgc gtaacgcact cgagaaggtg gtgtttcatg
     8581 tgaatcatgt tcctcgtcta gataagattg tatatttttc gggaaataaa aatctttgta
     8641 ccagaaataa acccaaacaa atcgacatgc ataaattgca atagcgatta atatagtgat
     8701 cactattaaa aatgaaaagt tgtcaggctc tgtttggaaa atatctatga ctacagtagg
     8761 aatcataaaa cctaatacaa caaatacaaa accatttaag gcataactaa atgtgttcca
     8821 aatttgatgg taattcattt gtagttccgt ttgtgctcta attaaacggt cgcgttctaa
     8881 accatggatt agacctgcga ttacaactgc aatgatacct gaagcatgaa cttcttctgc
     8941 taaaaagtat acgacaaaag gagttaataa ttgaataaaa gttaaggtat tgttatcttt
     9001 taaaccttta ttagcggtta agtctatacg tattctaacg acaacgaatc caataattgc
     9061 accaataagt acacctagta ttgttgaaat gataaattgt tcaacagctt ggaataatga
     9121 aaaggtacca gttactaatg cagtaacagc aattttaaat gaaatgatac ctgctgcatc
     9181 attgagtaaa gattcacctt ctaaaatcgt catagaacct ttaggtaata attttccgcg
     9241 tgtaatagca gatactgcta ctgcatcagt aggacataaa attgctgcta ttgcaaaagc
     9301 ggctggcatt ggtaaggcag gccaaatcca atgtataaaa tagccaacac cgactacagt
     9361 tgcaaacact agtgccattg acattaatag tataggttta cgatattcta ataattttgt
     9421 tcgagagacg tgggtacctt ccacaaaaag tagtggcgcg ataacggcaa acataaatac
     9481 ttcagaattg aattggaaat caacttgtat tggaataatg aaaataacga cacctaatgc
     9541 aatttgaata aaggcagtag gaatttgtgg gaatcgatta ttgataaccg aactaataat
     9601 cacagcaaaa ataaaaatta aaaatgcttc taatagtgcc atacaatact cctcaaaatt
     9661 ttaatagtta atattttatc acttttaagg cataatgaca tagatatatt gataaaatga
     9721 agttattttc aaaaaaactc tagtatcggt tgaactgata ctagagcgag atgtttaaat
     9781 tattgattgt catatctgaa atgaccgctg tcattttgtc gttgttcata cgcgagcttt
     9841 tcagcattcg ttttgtattt tttataaaag aaaaataaaa atataaacca gaatggcgaa
     9901 atataaatag ctgctcttgt ttcgtcacta aagaataata aaatgaatac aaagaagaag
     9961 aacgctagaa taatgtaagc aataggctta ccaccaatca acttaaattt actgttttta
    10021 tgtgcctcag gatgcttttt caaatacatt agataagcga caataatcat tgcccaaaca
    10081 actaaaaaca acacagttga taacgttgta acgtatatga atagttgaat tgcattaggg
    10141 aaaatgtagt ttaacaaggc tgctataagt aatgcaattg atgaaactaa cattgataaa
    10201 taaggcacgc catgactatt cgttttattt aaaactttag gacctaaccc ttgttgtgac
    10261 agtccgaata agatacgact attcgaatag ataccactat ttgtagcaga ggccgcggca
    10321 gttagcacga caaagttaac gacacctgct gcaaatggta cgccgattaa tgtaaagagt
    10381 ttaacgaaag ggctactatt tggatcgata tcattccaag gtatgactga cataattact
    10441 aatagaccac cgatatagaa taataaaata cggataggta cattattaat tgctttcggt
    10501 aaggtttttt caggatcttt cgtttcacca gcagttacac ctataagttc aataccaatg
    10561 aatgaatata cagcaatttg gaatgacatt aagaaaccaa atgttccacc agggaacatg
    10621 ccaccgtgac tgattaagtt tgtgaatgat gcatgtccat aatgtgtttt aaatgagaaa
    10681 aagattaata caagaccaac gataaccatc gcaataattg tgacaacttt aataatcgag
    10741 aaccaaaact ccagttcacc aaataatctg gcacctaata agttgaagct aatcaagatt
    10801 aaaacaataa atagcacggt aatccaattt gggacttgtg gataccaaaa tgcaaagtat
    10861 tgtcccatcg cagttaggtc tgacatactt gatacaatcc aacataacca gtaagtccag
    10921 ccaatgacaa agccaccaaa agggcctaaa tattcatttg caatatcaac aaatgaatta
    10981 aatcttgtat tgctcaacaa caattcgcct aatgcgcgca taaaagcgaa tagtacaacc
    11041 ccaataatca tgtatgtgaa taacagtgat ggaccagtta aagatattgt ttgacctgat
    11101 cctaggaata acccagttcc aattgcgccc ccaattgcta ttaattggat gtgtctgtta
    11161 ttcaactctc tatgcaattt tcttgccata attttcctcc tcaggataaa gcaaataaaa
    11221 aagttgtgtg taagcgtttt cttaattata aatactaaag aaatccattt aataaagcaa
    11281 ttattttttt aaatataaga aaaaacattt gataggtaag ggtatgataa ctaaaactta
    11341 tcgatttttt gaaaagatat aagtttttat gcaaaaatat aaaataatca tctaaatgaa
    11401 gcagttaact atatatatta gataagctat ggcataataa ataacatttt aatttgagta
    11461 aagaaggtaa gggtatgaag atagggacaa tcgccgattt acatattgat cgtcataata
    11521 aaaaaacatc cgaagattac ttagaggcat tggttgaaat agttaagtat aagaaattgg
    11581 atatattgtt gattgcgggg gatatctcaa atcattatca attgacgcac caatttataa
    11641 cgcaattgac aaaacaatta gatatacctg tgaaatttgt acctggaaac catgatttat
    11701 gggaagttga aagtatgact acgcaagaca tttggaataa ttataagagt atgtcacagt
    11761 gcttggtagg aaaaccattt atagtaaatg aagaatgggc aatcatagga catactggct
    11821 ggtatgatta tagctttgca gcacaacgat tttcattaga tgagttacaa aaaggaaaac
    11881 attatggtgc gacttggcaa gataaagaac gaatatcttg gggcatatca gatcaaaatt
    11941 tatctaaaat agcggctgaa caagtgaaga aagatatatt agaagtagga aatagacgag
    12001 tgattttagt cacacatgtt gtgacgcacc ctgatttcat tgttcctatg ccgcatcgta
    12061 tattcgattt ttataatgca tttattggga caagtgattt caatcctttg tatgcgatgt
    12121 tcgatatacc atatagtatt atgggccatg ttcattttcg taaaagtgtg atagatgatg
    12181 gcagatgtta tctctgtccg tgtctaggct atccaagaca atggcgttca gaagatattt
    12241 accaggaaat aaatgagacg atacaaataa tagaaattta aaatgcgcaa acctgaccca
    12301 gtttgcgcat tttatgtttt acacacgcga gtaatgtgtt tacttacgtg tgtttatttt
    12361 gttgctgatt ttcaattgta tatgaatgtg gttgcacata aatgcacttt cttcctggtg
    12421 aattaaagct gtattccatt ttctctttac gaattttaat aatttgtttg cgatttggaa
    12481 tgatggcagg taaaactagg ccacgacgaa tatgactcca aagtggatcg atataatagt
    12541 aatagtaatc atcgtaccca atgagtaacg ttacgtgaat atttgaaact tgtttcgctg
    12601 gcttgttatc aaagcggaaa acacgtagta atggtttaga accaagatta gtatggtata
    12661 ttaacacagg ttgaccttga tcgataatac ctttaagatc ttctaacgat ttaccagtgc
    12721 cgtctacgat attaggattg tatttttgta aaaatggtac atatgcttct ggaaatatcg
    12781 tttgatgata attgccaagc ttaatgaata agtgatgtcc aacataacct ttatgtggat
    12841 tgttcggatg tgtcggccaa tgtctcataa tttctgtagc agggatatgt tggttgttgt
    12901 attgcaacat catggctgcg gaaacacctt cacaccccat gaccataggg ataggaaata
    12961 gctgactgat aggtttaact ggtaatattt ttcggttcat aatatagtcc tcgcattgat
    13021 tcaataaata tttaatataa ttatatagcg tcaatgcaaa atgtcctaaa catatgtttt
    13081 acatgagtga ataaaattaa tggagtgata aaatggaata tcaattacaa caacttgcgt
    13141 cgttaacgtt agtaggtatt aaagaaacgt atgaaaatgg acgacaggct cagcaacata
    13201 tagcagggtt ttggcaaaga tgttatcaag agggagtaat tgcggattta cagttaaaaa
    13261 ataatggtga tttagccggg atacttggct tatgtatacc tgaattagac ggtaagatgt
    13321 catatatgat tgcagttacc ggagataata gtgctgatat tgaaaaatat gatgtcataa
    13381 cattagcaag ttcaaagtat atggtatttg aagcacaggg cgcagtacct aaagcagttc
    13441 aacaaaaaat ggaagaggtt catcactaca tacatcaata tcaagcagat acggtaaaat
    13501 cagcaccatt ttttgagttg tatcaggatg gtgatactac aagtgaaaaa tatattacag
    13561 aaatttggat gcctgttaaa gggtgattga atagaaatgt ccttgctatg aaggcgtaat
    13621 tttagttgaa gattcgatct cataattcat aaagaagaac cctgacaaag atagatatct
    13681 ttgctagggt tctaattatg ttgaagtgtt atgcgacatg ttgcttttta atcattttaa
    13741 agtaaatgat ttgtagactg actaatagaa tggctgtaat gaagataata atcatatatg
    13801 gtgatgtatt aaattcgcct tttaagccaa ctaatggtgc aacagcgcca cctaagataa
    13861 attggaataa gccaagtaaa cttgatgcgt taccactgcc acctgttcgt tcttccatag
    13921 ccattgtgaa accaagcggt ccaattgacg tcacaggaca cacatttaag aagaatgcga
    13981 tgagtaagac ccaaagtggt aaatggaatg taagtgtcag gataattaaa gcaacaccta
    14041 ccacttgtat aatagttaaa atgattaata atatgtggcg atgtaatttt tctactaata
    14101 aagcaacgac ttgactgaca atgattaaac ctacaccgtt aacagcaaac atgatactaa
    14161 attgttgggg tgtcatatta tacaattttt gagtaataaa tggcgatgca gatgaataac
    14221 taaatagcat tacataagtt aaaccttgta atagcattgg aataataaat gctggttttt
    14281 tcaaaagact tccaaaatct ttaatgacgc tactaaaatt cacctgcttt aattcatctt
    14341 tagatgtttt aggtaattga gaagcgacgc caattaaaat gatgagtgcc acaatagtta
    14401 aaattgtgaa aatagaacgc catgttgcta cggataaagc taatccaccg gctaatggtg
    14461 caagaatagt gatgatgcca ttaactacca ttaaagatgc taaaaattta gcgagtgcgt
    14521 tgccactaaa tttatcacca gcagaggctt ttgcaatcac gatgacgcca ccaccagtta
    14581 aaccttgaat aaatcgtaaa gttaagaata atggcaattg atcaacaaac atagacaaac
    14641 ctgataccaa aatgaaaatg atcaaaatgg atacagcaat ccgtttgcga ccaaaagcat
    14701 cggataaagg tccaaagata aattggccta gtgcaagacc aatcattgtg aatgataatg
    14761 ttaattgaat ttctgatgta gtagaaccaa aatcaccttg cactttaggt aatgatggta
    14821 cgtacatatc tatagttagt gggccaaaag cggtcatgac gcctaacgta attagcaata
    14881 aaaatgaaaa atgcttcgtt ttggttttcg ttgattgcat gatcagactc ctttaaatga
    14941 tataattata taaattatat acgttttgtg tgtatttgta tgtacttaga atacaagttt
    15001 atataaaaaa tccctatact cttaatgcat catcacgaat aacgtagtga tacagataaa
    15061 gtatagggaa ttaatgttta tcatcaaagc aggtgtaata attaattagc gccaccattg
    15121 tcatctttaa ataagctttc taatttagat ttaatactgt cgaattttgt attaatatca
    15181 tttaacatgc tttcaatttt accttggctt gcattgtttt taagtgcatt atttgcctct
    15241 gtcaatttgt tgatttggtc ttgataaaat tgagttagct tagaatcagc tttgttttgt
    15301 tcttgctgct tcaagttgtc gatttgattt tgtaaatttt taataatgtc atcagttttt
    15361 tggttgtttt taatattttc tttagcatta tctacttgct ggttaatttg gccgtcttga
    15421 ttttcaactt gttttttata attttgagag actttagggt cattactaac tttatcgcta
    15481 tcattgttaa gtaaggcgcg tgtgacaaaa atagcgacaa taattaagag aatggcaata
    15541 atgatatata accatttttt tgaacggcgg gatttctcgt tttcttcttc aagaatttgt
    15601 tctcttcgat aatctctacc attatagtat cgttccgggt gttcttcagg atcgccttta
    15661 gcatattgtt gtctatacga tgcgtcttca gattgacgac gatgattttg gttttgattg
    15721 tcgtattgat atgaatcacg gtatttatca gttcttttca tgggtttcac ctcattcaag
    15781 ttttaactgc aaataataaa gctgatgatt tactttgaca ttattgtaat ttcagaatac
    15841 gagcaatgtc agttgcagtg tttaataaat tttgttcgct attttgtagg acatcttcta
    15901 aagtgctagg tgaagagatt atagaatagg cactatcaat accgaaatcg taaacatgtt
    15961 gataattttc gcctagactg ccacaaatcg cgatgacagg aatatgatat tgttttgcag
    16021 ctaacgcaac gcctacgggt gttttaccaa agatggtctg ataatccatg cgtccttctc
    16081 cagtaataac gaggtctgca tctttaattc tttgatgaaa atctgtaatg tcaaagacga
    16141 catcaatacc ttttgttaaa gttgtctcac aaaacgctaa taatgctgcg cccataccgc
    16201 cagctgcacc agaacctggt atttgattaa cggactttcc tgtgcacatt tttatcttat
    16261 catgataatg cgacattgcg aaatccaact ttggtatcat ctttgcatca gcgccttttt
    16321 gaggaccata aatataggta gcaccatttt cacccaataa aggatttgaa acatcacagg
    16381 ccactttaaa ggtcacctct tttaatcgcg aatctagatt ggttatatcg atttgtgcaa
    16441 tgtgagcaag attagcacca ttcatttgta ataagtcccc gtttacatca gtaaacttta
    16501 cgcctagtgc acttagcata cctgtaccac catcatttgt tgcactgcca ccaatcccta
    16561 aaataatggt cttagcacca tgatttaatg catctttaat tagttcaccg gtaccatatg
    16621 atgatgtgta taaaggatta cgttcctctt tttctaataa atccaaacct gacgctgccg
    16681 ccatttcaat aattgcaatt tgttgttcgt ctgcgcgtgc ataacatgct tcaataggtc
    16741 gcattaaagg gtcattaacg atgactgtat acttagtggc acctgttgca tgcattaaag
    16801 catctgtggt accttcacca ccatcagcca tcggaatgat atcataatga agggtattcc
    16861 cataaacatt agtaaaagcc tgttttataa tattgccaac ttgctgtgcg gtcatacttt
    16921 ccttaaatga gtcaggagcg atgacaattt tttgtaattg catagtagac atcctctcat
    16981 tactgttgtc gctcttattg tatataaaat tactagtgaa ctaaagtaat ctctatagtg
    17041 ctaatgagtc acgcgataaa tcagttacat tataatggcg tataaggatg aggggaaaga
    17101 gactaaattt gctgtgaaag ggtataaaga ttgaaataag catatttgaa gtgaagcgga
    17161 ttggctcgca tattcgagta tgttcgagaa cgtagttatg catatatttt gatgttgagg
    17221 agttacacat atgaaactaa cacaaacgca tgctgaaatt ttaaaattta tcattgttgg
    17281 cggcattaat acgttaaatt attatgttgt ctatttattg ctgttaaaat tattacacat
    17341 tgaatatatg attagtcata ttaccggatt tctagtcgct tttgtgattt catattattt
    17401 gaattgttat tttgtttaca gggtaaaacc tacttggaga aaattcatta gttttccaat
    17461 tacgcagatt gtcaacgtaa gcttacaaac agttttatta tatgtatttg tatcctggct
    17521 aaatttgcca gcagaaattg caccatttgc tggtttaatt attacgatac ccatcacatt
    17581 tatattatct aaatggattt taaaagatag caattaaagt agcgtaaacc actaaaaaaa
    17641 actttactta agccttaata ttgcgtgaaa tataataaat aaatgatata atatgatggt
    17701 cgcctaacat ctcgggcgtt actttaacgc agagaggagg tgtatgacgg atgttcaatt
    17761 tattaattaa catcatgact tcagctataa gcggctgtct tgttgcgttt tttgcacatt
    17821 ggttacgaac gcgcaacaat aaaaaaggtg acaaataacc aacacctaaa taaatcccct
    17881 cactatggca gtagtgaggg gatttggtgt atttagatgt tcaatttatt atgtaattaa
    17941 ttatagcatt gatgaacaag gtgtgcaatg tatttttgca atattacaat aaatgaattt
    18001 aattatatta gaattaagta tttcgaaaaa tgatttaata gttgagtcat aggatcatta
    18061 gataaagcga ataatcaatt atagggacat tgggatacaa atcaatgtac tagactctac
    18121 aaggctatat tcagggaccc aacaaagaga aattggattc ccaatttcta gagacaatgc
    18181 aagttggggt gggacgacga aataaatttt gtgaaaatat catttctgtc ccactcccag
    18241 aaccactaca taataaatca ttagtggctc tttatcattt ctgccccata accttgtgaa
    18301 tttttaaaag aaatgtgtca caaatccacg cgacattgat aatcgttatc aactaatata
    18361 tgatgtactt gtaataagta ttaattttt
//
LOCUS       DALAGF010000049        18321 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.049, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000049 DALAGF010000000
VERSION     DALAGF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18321
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.049"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            178..1176
                     /locus_tag="O2692_001530"
     CDS             178..1176
                     /locus_tag="O2692_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCZ4299163.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            1408..2289
                     /gene="rbsU"
                     /locus_tag="O2692_001531"
     CDS             1408..2289
                     /gene="rbsU"
                     /locus_tag="O2692_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HCZ4299164.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGLPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            2304..2708
                     /gene="rbsD"
                     /locus_tag="O2692_001532"
     CDS             2304..2708
                     /gene="rbsD"
                     /locus_tag="O2692_001532"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCZ4299165.1"
                     /translation="MKKSAILNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            2736..3650
                     /gene="rbsK"
                     /locus_tag="O2692_001533"
     CDS             2736..3650
                     /gene="rbsK"
                     /locus_tag="O2692_001533"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCZ4299166.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKVAHIDTSYIIKTAEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSIGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(3748..3882)
                     /locus_tag="O2692_001534"
     CDS             complement(3748..3882)
                     /locus_tag="O2692_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299167.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            3901..4662
                     /locus_tag="O2692_001535"
     CDS             3901..4662
                     /locus_tag="O2692_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ4299168.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH"
     gene            complement(5096..6532)
                     /locus_tag="O2692_001536"
     CDS             complement(5096..6532)
                     /locus_tag="O2692_001536"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCZ4299169.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(6548..7339)
                     /locus_tag="O2692_001537"
     CDS             complement(6548..7339)
                     /locus_tag="O2692_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCZ4299170.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDTQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVGIQSEDGVDIVIHIGLNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            7488..8192
                     /locus_tag="O2692_001538"
     CDS             7488..8192
                     /locus_tag="O2692_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCZ4299171.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(8300..9001)
                     /gene="lrgB"
                     /locus_tag="O2692_001539"
     CDS             complement(8300..9001)
                     /gene="lrgB"
                     /locus_tag="O2692_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HCZ4299172.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(8994..9437)
                     /gene="lrgA"
                     /locus_tag="O2692_001540"
     CDS             complement(8994..9437)
                     /gene="lrgA"
                     /locus_tag="O2692_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HCZ4299173.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(9550..10290)
                     /locus_tag="O2692_001541"
     CDS             complement(9550..10290)
                     /locus_tag="O2692_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HCZ4299174.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNNASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPTYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(10293..12047)
                     /locus_tag="O2692_001542"
     CDS             complement(10293..12047)
                     /locus_tag="O2692_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCZ4299175.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(12294..12968)
                     /gene="scdA"
                     /locus_tag="O2692_001543"
     CDS             complement(12294..12968)
                     /gene="scdA"
                     /locus_tag="O2692_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HCZ4299176.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAAALEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQSAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGELLVKMSELTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(13108..14832)
                     /gene="tarS"
                     /locus_tag="O2692_001544"
     CDS             complement(13108..14832)
                     /gene="tarS"
                     /locus_tag="O2692_001544"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCZ4299177.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKNQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFKGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTA
                     HITYPLNK"
     gene            complement(14865..16553)
                     /locus_tag="O2692_001545"
     CDS             complement(14865..16553)
                     /locus_tag="O2692_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCZ4299178.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(16575..17600)
                     /locus_tag="O2692_001546"
     CDS             complement(16575..17600)
                     /locus_tag="O2692_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCZ4299179.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(17593..18309)
                     /locus_tag="O2692_001547"
     CDS             complement(17593..18309)
                     /locus_tag="O2692_001547"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HCZ4299180.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
BASE COUNT         5868 a         3434 c         2790 g         6229 t
ORIGIN      
        1 ccttaataat atattctctc gtctcaacct taatcgtata cttcagacgt ctgtttgtag
       61 acaataaaag tcattcacgt cttcatatgt catcaaatgt ttatcatgat atgatgaata
      121 taataatcgg gtatataact gtatgattaa ttacacaata aagagaagga atataatatg
      181 aaaaaagtgt caattaaaga tgttgctaga gaagctggtg tatcagttac aactgtgtca
      241 catattttaa atcataatga tagtcgtttt tccgcaacaa cgataaaaaa cgtacatgct
      301 gtttcagaac gtttaggcta tgcccctaat aaacatgcaa aacaattgcg cggcagtaaa
      361 attcaaacta ttggcgtcat tttgcctagc ttaacaaatc cgtttttctc agcactgatg
      421 caaagtattc atgaccataa accatctgat gttgatttat gctttttaac atctacagca
      481 actgatttgt atgacaatat taaacattta attgatcgag gtattgacgg attaattatc
      541 gcacaataca tatcatcccc ggacgcccta aataactatc taaagaaaca tcatgtacct
      601 tatgtcgtac tggatcaaaa tgaccatcaa ggctatacag attttgttcg gacaaatgaa
      661 tatcaaggtg gacaacttgc agcacaacat ttaatagaac tcggtcacaa ccatatgata
      721 attgttgcac catatgacat gatggcgaat atgtcgactc gtgtcgctgg atttgtcgat
      781 actttgcgcg cgaatcaatt gccagaacca caaatcgtcc atactgaatt atctaagcgc
      841 ggtgggctaa ccattgttga tgacatcatg gttcaatctg ccactgcaat cttcgctatt
      901 aacgatgaac tcgctattgg cattttacga ggactaattg aacatggcat cagtatcccg
      961 aaagatatct cattaatagg ttatgacgac attgattatg cagcgtacgt ctcgccacct
     1021 ttaactactg tggcacaacc tataactgat attggcaaaa catctttaac cttattactt
     1081 caacgattac agcacttaga taaatccatt gatatgattg aattaccaac gactttaaaa
     1141 attcgtgcaa caactggcta tcatctttca aactaactac gtatcttccg aaatatactc
     1201 atcattgtta ggcccttagc gttgctttaa tgctgagggt ttttaatcat aattatttta
     1261 ctaagaaatt aaaataataa tgtatgaatt tttaaatatg atttaaacgt tttcagtttt
     1321 tatgaaaacg catgcatttt acaaataaaa atggtacgat ggcactggta aaacgtttta
     1381 ctaaaaacaa atcatgaggt gtataacatg agcattgttg cattacttat cgggttaggc
     1441 cccttaattg gctggggctt cttcccaaca gtcgcttcaa agtttggtgg taaacctgta
     1501 catcaaatta tcggtgctac tgtaggtacg ttaatcttcg ctattatatt agccgtagtc
     1561 acatcaagtg gcttacctac tggaaccaat ttgctattcg ccttattatc aggtgcagga
     1621 tggggattcg gacaaatcat tacatttaaa gcgttcgaat tagtcggctc atctcgtgcc
     1681 atgccagtca caacagcatt ccaattatta ggcgcatctt tatggggtgt ctttgcatta
     1741 ggaaattggc caggcattgg tcataaaatc attggattta cagctttagt cgttattcta
     1801 attggagcgc gtatgacagt ttggagtgaa cgcaaagaag caagtaacgc caaaaattta
     1861 cgtcgtgcag tggtacttct gttaattggt gaatttggat actggttata ttcagctgca
     1921 ccgcaagcaa cttctattga tggcctaact gcctttttac ctcaagcaat gggtatggta
     1981 attgttgcag tcatttatgg ctttatgaat atgaaagcag agaatccatt ccgtaataaa
     2041 attacgtggt tacaaattat ttcaggtttc ttctttgcat ttggtgcttt aacatatctt
     2101 atttcagcac aacctaatat gaatggttta gcaactggat ttattctttc tcaaacatcc
     2161 gttgtgcttg ctacattaac tggtatttat ttcttaaaac aacataaaac gtcaaaagaa
     2221 atggttatta caatcatcgg cttagtactc attttagtag ccgcttctgt tacagtattt
     2281 ataaaataag gagtgtagat gtcatgaaaa aatcagctat tttaaatgaa catatttcaa
     2341 aagcaatcgc gacaattggt cattttgatt tattaacgat taatgacgct ggcatgccaa
     2401 ttccaaatga tcatcgtcgt atcgacctag ctgtaactaa aaacttacca cgctttattg
     2461 atgtcttagc tacagtgtta gaagaaatgg aaatccaaaa aatatactta gcagaagaaa
     2521 taaaagaaca taaccctaca caattgcaac aaattaaaca attgatttca tcggaaatcg
     2581 aaatcatttt cattcctcac gaagaaatga aaagtaactt agctcaccca ttaaataaag
     2641 gtaatattcg tactggtgaa acaacgccct actctaatat tgcattagaa tcgaatgtta
     2701 ctttttaaaa gttataactt gaaaggagcg tacacatgac caacaaagtt gttattttag
     2761 gttcaacgaa tgtcgatcaa tttttaacag ttgaaagata tgcacaacca ggcgaaacat
     2821 tacatgttga agaagcacaa aaagcattcg gcggaggtaa aggtgccaac caggctattg
     2881 ccactgcacg catgcaagca gacacaacat ttattactaa aattggcact gatggcgttg
     2941 ctgatttcat cttagaagat tttaaagtag ctcatattga tacatcatat attatcaaaa
     3001 cagctgaagc aaaaacgggc caagccttta tcactgtgaa tgcagaagga caaaacacca
     3061 tctatgttta tggtggtgcg aatatgacga tgacacctga agatgttatt aacgcaaaag
     3121 acgctataat caatgcagac tttgtcgttg cacaattaga agtacccatc ccggctatta
     3181 tatctgcatt tgaaattgcc aaggcacatg gtgtgacgac agtattaaat cctgcaccag
     3241 cgaaagcatt acctaatgaa ttattatcat taatcgatat tattgtgcca aacgaaacag
     3301 aagccgaatt gttatctggg attaaagtaa ctaatgaaca atctatgaaa gacaatgcca
     3361 attacttttt atctataggc attaagactg ttttgattac gctaggtaag caaggtacat
     3421 attttgctac taaaaatcaa agccaacaca tcgaagctta taaagtaaat gcgattgata
     3481 caactgctgc aggcgacaca tttattggtg catttgtcag tcgcttaaac aagtcgcaag
     3541 ataacttagc agatgctatt gattttggta ataaagcgag ctcactcact gtacaaaaac
     3601 acggcgcgca agcatctatt cctctactag aagaagtaaa tcaagtttaa atgaatcaaa
     3661 cacagctatg atatgaaggt ttagcatata acatgcaaca ttcgtatatc atggctgtgc
     3721 ttttttatct ttataaaaca tcatctatta gaaataattt tccactaaac ctatgcttgt
     3781 tgactcatgt ttagttataa atgaagtgac aattttttgt aatcttttta acttccaaat
     3841 tatcgcatat aaatatgcta tattaatgat aataattatc aattaaaagg aggttatgct
     3901 atgtctaaag aagctggtca tacattttta gctaaattag gaaaaactcg tctacgcccc
     3961 ggtggtaaag aagcaacaga ttggttaata caacaagggg cattttcaca agataaacaa
     4021 gtgttagaag tggcatgtaa tatgtgcaca acatctattt atctagctca tacatatggc
     4081 tgtcacattc aaggcgttga tataaataag aaagcattag aaaaagcaca ggaaaacatt
     4141 tcagcagcag gtcttgaatc atatattcaa gttcaacaag cgaatgctgt taaattgccc
     4201 tttgatgaca atcaattcga tatcgtttta aatgaagcaa tgttaacaat gttacccatc
     4261 gccataaagg aaaaagcatt acgcgagtac taccgagtct taaagcctgg gggtatcttg
     4321 ttaacacatg atattgtcat cgttaatgaa tcacatgcca cacatgttgt taaatcatta
     4381 tctgcagcaa ttaatgtcaa tgtctcaccg cagacgaaac ttggctggtt agatttatat
     4441 aatcaagctg gttttaatca tgtgcattat catactggtc caatgagttt aatgacacca
     4501 aaaggtttaa tttatgacga aggtattgtt ggaactataa agattatcaa caatgctttg
     4561 aaaaaagaaa atcgaccaat gttttgtaaa atgtttaaaa cgatgactaa attgcgtaaa
     4621 gatatgaatt atattacttt tgtcgctaaa aaagagcact aaatataatg ccactaactg
     4681 tactttgtat ctatgtttga ctatcacttt aatttctttg tgacactaat catctactta
     4741 acaatatcgt tatcgttgat tagtaagtca tcaattttgg ttaaagactt tcataaacac
     4801 tcaaacatta acactataca tagttagtgg cattattttt ttcttaaaat tttaacatca
     4861 cgggattgga acagaaatga tattttcgca aaatttattt ctgtccttcc ccaacttgca
     4921 ttacctgtag aatttctttt cgaatttctc tgtgttgggg ccccgccaac ttgcattacc
     4981 tgtagaattt cttttcgaaa ttctttgtgt tgggggcccg ccattataac actgtaggtc
     5041 ctactatatt aatttatgtc tcgggctcta accctaaaat acttataata tacctttata
     5101 atttatctcc atttgatgct atcacttgtt gataccaata gaacgaatct tttttcaagc
     5161 gttccatcgt gccatgacca tcattatctc gatcaacata tattaaacca tagcgtttct
     5221 tcatttcacc ggttgtaaat gaaacaatat caatgattcc ccacggtgta taaccgatta
     5281 aatctacacc atcttgatca actgcttcta tcgctgctgt aatatgtgct tttaaatatt
     5341 caatgcgata atcatcatga atatgtccat ctaccacttc atcaactgca ccaaaaccat
     5401 tttccacaat aaaaagtggt aactgataac gatcatataa cacattcaat gtatatctta
     5461 agccatctgg atcaatcgcc caaccccaat cactcgttgc gatatgcgga ttctccacag
     5521 aatgattcaa accaccgttg acgatgttgt tttcaactgt agtatcaaca tcatgtttta
     5581 cagccgtaga catgtaataa ctaaagccaa tataatcaac cgtgccctgc tgtaaaatac
     5641 tgtcgtcccc ttcttgccat ccaacatcat atccttttcg tgccaacatt tttttagcat
     5701 agcttggata ataacctctc acttggacat ccgggaaaaa gaaacgtaag cgattcgcaa
     5761 tttgtgcttc catcatatct ttcggatgac acgaatatgg ataaatgggt acatgtgaaa
     5821 tcattgttcc aatcttaaac ttcggattaa tctctttacc aagacgaact gctaaagcac
     5881 tggctaaaag ttcatgatgt gctacttgat acaagacttc ttcaggatta tcattttctg
     5941 tcaatgctac cccagaattc gtccataaaa agataggatt tgatgtgtcc atctgattat
     6001 taatttcatt aaacgtcatc caatatgtaa ctttatcttt atatctttca aatacaacac
     6061 gcgcaaaatg cacaaaataa tcgacaactt ctctatttct aaatccacca taatgtttcg
     6121 ctaaatgaag tggcatctca aaatgtgata acgtcacaac aggttcaata ccttgtgcaa
     6181 ttaattcatc aaagatacga tcataaaagg cgagtccttc ttcatttggc acatcttcat
     6241 ccccattcgg aaagatacgt gtccacgcaa tcgacgtacg taaacatttc aatcccattt
     6301 ctttaaacaa ggcaatatct tccttataac gatgataaaa atcaatacct tcatgatttg
     6361 gataatagtg attgggatct atagattctg taatctgacg tgctttgcca tgtgcaccac
     6421 tcgtcataac atcaattaca cttaaccctt taccaccttt atcatatcca ccttcaaatt
     6481 gatttgcggc aagagcgcca ccccacatga aattttgcgg taattttgtc attattacaa
     6541 cacactccta gtttttaaat attttaaaaa taacatcttc agtcgtaatg atttcattca
     6601 ttgtcagttc tacttttcct aaatcggcag agttgctaat aacgacaata acgtcagcat
     6661 tgtagccttg ttgttgtata tattgctggt cgaattgcaa caacgtttgc cctgcttcaa
     6721 catgatcatt ttgctttaca aagcacttga accctttacc ttccaagtca actgtattca
     6781 agccaatatg aatgactatg tccacaccgt cttctgattg aataccaact gcatgcttag
     6841 ttggtacaat catagatatt aaaccattga acggtgcgat tactttggat tcttcatgag
     6901 ctttgattgc taagccttca cctaccattt tctctctaaa aattgaatct cttacttctt
     6961 taagtaaaat acgacgacca gcgattggtg ctttcactaa aattggtgtt gttactttta
     7021 cttgaggtgt ataacttgta tcttgactat taatattgtc agtattctca ctctcttcga
     7081 cattagcatt cttaattaat cgctgtaatt gctgatacac ttcatcgaca tttccattta
     7141 attttatgac taactgcgta tcattttctg ctacagatgt cacatcaact atttgtttca
     7201 cttcatttgc tgtagaagga attgtatgtt gcatatgaat tgtaatgcac ttcgtatcat
     7261 acgttgcatc aacaatgttt tcgacaccac ctacagctgt aagaatgtct tgggcttgtt
     7321 ctttatattt attgctcata aatattaccc cctcaacttg atagtagaat gaaaatgtat
     7381 ttacttacct ccaattataa ttgtaccggt tcaatttgta aacgccgata caattataat
     7441 attttgtgct ataataatta cagacaaagt gaaacgagga cagaatattg ttaaagtatg
     7501 aacatattgc taagcaactt aatgcgttta tacatcaatc taatttcaaa cccggtgata
     7561 aattgccaag cgtgacgcaa ttaaaagaac gttatcaagt aagtaagagt actatcatta
     7621 aagcattagg cttattggaa caagatggtt tgatctatca agcacaaggc agtggtattt
     7681 atgtgagaaa tattgctgat gccaatcgta tcaacgtctt taagactaat ggtttctcta
     7741 aaagtttagg tgaacaccga atgacaagta aggtacttgt ttttaaggag attgcaacgc
     7801 cacctaaatc tgtacaagat gagctccaat taaatgcaga tgataccgtc tactatttag
     7861 agcgattaag attcgtggac gatgatgttt tatgtatcga atattcttat tatcataaag
     7921 aaatcgtgaa atatttaaat gatgatattg ctaagggctc tatcttcgac tatttagaat
     7981 caaacatgaa acttcgtatt ggtttttcag atattttctt taatgtagat caactcactt
     8041 caagtgaagc ttcattacta caattgtcta caggtgaacc atgtttacgt taccaccaga
     8101 ctttttatac aatgactggc aaaccctttg attcatctga catcgtattt cattatcgtc
     8161 atgcacagtt ttatattcct agtaaaaagt aataaataca taaaaacgtc tatatcccag
     8221 ttataaactg gagtatagac gtttttttac gataataaca atggctcaaa ttgctattat
     8281 cttgcttagg tttttcgttt tagaagaata ttgctacaaa gacaggcaca actgctacaa
     8341 caactacacc aactaacact aaagctatac ttgccattga ttcttctaca ggtcctaatt
     8401 ctttggctgg tgctacacct aatgtgtgac cacttgttcc aagtgctaat cctcgggcaa
     8461 tagggttagt aattcggaaa agctttaaga atttattacc tagggcataa ataatgacac
     8521 catttaaaat aactgctaat gatgttaatt cttttatacc accgatacca gctgatactg
     8581 gtaacgcaat cgctgtagtt gctgcttgag gtaacattga taaaataaca tcattggcaa
     8641 attgtgctaa cttcgcaaaa gttaaaataa ttaataacgc tacaactgta ccgataccaa
     8701 tacctccgat gatacgatgc caatgtttaa caagcacttc acgcttttta tataacggaa
     8761 tcgcaaaaca gattgttgcc ggttctaaga agaagtaaat aatgtctcca cctattttgt
     8821 aagtcttata cggaatgcct gttaaataga ggaaggccac accaaatacc atactgacaa
     8881 atagcggtgc gaataagaag aaacgattag ttttttcaaa taatatggtc gctaagaaaa
     8941 atggtataac ggataacagt attccgaagt aaggtgtgtt tagtgctaag tggttaatca
     9001 tgagcttgtg cctcctctat tttgatcttt tttgtgactt tgtcaccttt agatctcgaa
     9061 gtaactttca taataatttg tgtgacatag ccagtacaaa taagtaatag tattgttgag
     9121 acgattatta gtccaatgat taaaaatggt gcttggctaa tgacacctaa agagttaaca
     9181 actgagatac cggctggtac gaagagtaag ccaatgttat ttgttagtgt cgttcctact
     9241 ttttcgactt cgcctaactt aacagcacca gtacataata atacaaataa taatactaaa
     9301 ccgattactg atgcaggcat aggaattggc ataaatgatt caattatttt cgatacaaag
     9361 agtactaaag caattacaat gacttggtga aaaaagtgtg ctggttttga tgcgtctttt
     9421 tgttgtttca cgaccattgc ctcctacgtt tgatttaact aaagtataga tggctcactt
     9481 cgatttgcgt gatttttagt ccgaaataca aaatatcata ggtaaaatgc ataaaaaaaa
     9541 ggattactgt taaagtaatc ctatcgacgc tttaaaatct ttcataaatg aacgtccaac
     9601 ttgcatcttg acaccatttg tcaatattac catataagta tagttaaacc attgttgcac
     9661 ttctttaatg tgtttcgtgt taataatata tgaacgatga atacgtataa aataagtggg
     9721 attcaatcgt ttttcataac gattcaatgg ctctgttgtt tcgtatttat gattcgttgt
     9781 atgtatggtt gtaataccat tatgtgtgcc aatcccaata atattttgtt gctttaacat
     9841 gtgaatttta tcgtcaattt caacaggtaa gctttgatca aaattcgccg acatatcatt
     9901 cgcaattgca cttgcgttat tatcatcttt ggctttagtc gcacgcactt tattgactgc
     9961 ttgttcaata cgtttttgac caaacggttt caaaatatag tctgtcgcat ttaattcaaa
    10021 tgcctgtact gcgtattggt catgtgcagt tgcaaaaata atcgcaggtg gctctttcat
    10081 cttttgaatc ttagctccta attcgatccc attttcatcc attaaattga catctaaaaa
    10141 tataatgtca tattgattga tcagtagtgc ttccaatgtt tcttttacat tttctgcctc
    10201 attaatttct tcaaaaccac caatttcatt taataaatat gttaattcat tacgtgctaa
    10261 tggctcatca tctatgatta atgctttcat atttattcct cctcttgtct ttcataagga
    10321 agtacacacc aaaaagtggt accgctcgat gtcgattcaa attgtaatgc tgcggatttt
    10381 ccaaataatc cttttaggcg taagtttaaa ttttctaaag cactaccagt tccagactct
    10441 gattctacag atgtttctcc caacaaatgc attttatctt tagaaatacc ctgaccatta
    10501 tcttgtacaa taatacgtac atgtgttgca gtttctttaa tcactgacac atcaatatcg
    10561 ttaccttgct ttcgattcgt aaacgcatgt ttgatggcat tttcaactaa aatttgaatt
    10621 aaaaatggtg gtacaagcac atcgcgatat ttgtcttcaa cattaatatt gatattaaat
    10681 cttcctggat aacgtgcttg ttcgagtgat aagtatgcac gcacttgact taactcttta
    10741 tctaaagtaa tcgtatgttg cttagagcct tgtaaattcg ctctgaaaaa ataactcaat
    10801 tctaatagta actctcgtgc cttttcgcta tttattctaa ctaaagctga gatcgtgtta
    10861 attgaattga agaaaaaatg tggactcact tgtgcctgta atgacttaat ctcagcatct
    10921 ttcaataact tactttgcgt ttcggcttca ccaagttcaa tttggctact aaaaatattt
    10981 gccaatcctt ctgcaagttg acgttccaca aaagttaaat cattagggtt tgtaaaatac
    11041 atcttcaatg taccgacgat agaaccatgc atctcaagtg gtatcacgat agctgctcta
    11101 agcgggcaat tcggatgact acaaccaatc tcttctttag tatgaacttc tttcaacttt
    11161 cctgatttca atacatcttt agacagactt gttaatattt catttgttgg tatgtgatga
    11221 tcactacctg cacctacatg cgataagatt tcatttttgc ttgtaattgc tacggcagat
    11281 actttcatta aatttttaat aatcatcgca atttgctgtg ccgattctct attcaatcct
    11341 tctttaaaat acggcaatgt ctggttcatc aattgcagta catcatgtgt ttgaacagcc
    11401 ttcatttgct cctcttgctt taatgttgaa atgataatag acataaaaat cgccgtacca
    11461 acgctattaa caataatcat tggtagtgca attaatgata tgaggtcaac cgcatatgct
    11521 ttgtcgtggg aaaatgttaa aatgctcaac atttgaatca tttccataac aattccaatc
    11581 atggcacttt tcgcaatact cgggtaacgc ttgcgtcttt gagcttgtaa gccaaaataa
    11641 ccagcaatta taccaataaa tatagatgag ataagataaa cttgtgcatc cgccccaccc
    11701 atatacactc tgaaaatacc tgaaataacg ccaacaaata gacctacaaa agggccacca
    11761 actaatcctg cgacacctat cgttaataca cgtgtgttag ctaaagatac atcatcatct
    11821 aaacggaagt acacacttcc tgacaaacta tgttgatgat cgatgacgat accagttaaa
    11881 ttagacatta aggcaaacaa actgaaaata atacataatt gccaacgtgc tttccatgta
    11941 cgtcgacgat tcattaagtt tttaaaatat ggaatattca tcaacacata ggccaaaata
    12001 ataattaaac ctacacgctc aagtaataac attgttagcg atagcacgat agcacctcag
    12061 tgaattataa ttatgtttat tttatcataa tagtgcatgt aaaattcatg aataaattat
    12121 taaaaaaaca cgctactatt taattatcta taccattaaa ttttcatatc aatttttatg
    12181 ctaagttgac catttttctt ttcaaataat gctgtttatg tcactattat gacttttata
    12241 catagcgaaa aactgctgga cttatttttg ccaacagttt ctaatcgcgt tatttatgat
    12301 acttttttaa ataatacgtg attctctaaa tgtacgtgtt catgtgttaa cacttcaagt
    12361 gcttttaatc tctgataaac aagtcgccaa gtaccacacg cttctatcgg aggttcataa
    12421 ctagatgtta attcgctcat ttttactaac aattctcccg ttgcaatgtg gtctgaaact
    12481 aaatcatcta tcacagtatt aatatcgtct actacctcac cttgctcata tttaatgagt
    12541 tttggaaaat cgacatcgtc ttctttttgc atatgctcta acatgccatt tttaaatgta
    12601 tcgtatgttt cttttaactc aactaaatat ggatgattag gtccatgtac tttcgataac
    12661 ttcgtcacat aaggtgttaa atttttaaat tcttctctta gaggttcatg atatgctgat
    12721 tgaatatatt gaataagtga tgaaacattt aaaaatttag gatttaacga acctggtgta
    12781 ttcgtttgtt caacgtcgtt gagacgctgt aataattcgt tcaaatctac atttttcttt
    12841 tctaaggctg ctgcttctat acttacttgt ccgccacaac aaaaatctat tcccacactt
    12901 ctaaaaatat ccgctgcttt cggataatca gttactacat ctgctactat gtcattttta
    12961 tttatcatta tagataacct cctcatttcc agtgtataca atttaatgta tatgaaaatg
    13021 aaaatcaggg aaatgtcata atcatgtcat aaacagcaaa ataaagccct caaatcatgt
    13081 tggctcacaa tgatttgagg gcatttatta tttatttagt ggataagtga tatgtgcagt
    13141 gtttaactta acaatatttt tgaagtctcg ctttttaata tctttgctaa gtaagatttg
    13201 cacttcgttt tctgatgttt ggcttaacat tcccatcacc atgtcgtctt ttagtacgac
    13261 actagccata ccttcttcaa atacataacc acgatccaga ccagttaaaa tgattttgtt
    13321 tttacctttt aacttcaatg agattgaatc cgttaatgta atagctgtaa agtataacga
    13381 aatgttatta aagtcttttg tgaaatacgg tgttaatcta taaaaatcat tattcaaatg
    13441 aattaatgca cttgtttctg cagtatatgc atatttatta cgttgattac caacacgcac
    13501 ttcaaatact tccgtaccta tttggacttc taagaaaaca tcccaaattt tttctttagg
    13561 taaatacgct ttaatatctt ctaacataat gttaaaacga tattgtttat tcgcattgac
    13621 acttaaaggt acatacattg tgtgcttctt gtttcgtgaa tgtaatttaa gtctgacatc
    13681 ataatgtcct tcgccaatca taaattcatt taacgttagc tctgtaacaa ttttatgatt
    13741 atcaaattta aatgctgtca ttttcacttt tggcttagcg atatcaatgc ctttaaagta
    13801 tggttcgcct tcattgaatt gaatgtttaa tttaccatct acaatatcaa aacggtagta
    13861 ttgaccttga cggtaacttt cctccacagt tctatagttg tctatatctt tcgctctcgc
    13921 gtagtgcaac aatggtcgta atttactcat caccaatgca tctacacgtt ctggaactgc
    13981 ttgtataaag tctcctagag cattaatcca ttgtggttga tcttctagtt taactttaag
    14041 tgagaagcca ttcgtacgag aaaaactaaa atgacgattt aaaaattctg ctaagatttg
    14101 atttttatgg gcttcttcta aatctgcatt taatatttct acagcaatca atctcatgac
    14161 ttcataaaag tcttcaggtg aaacatacgc actactcata tgttcacctt cacgctttgt
    14221 agcataataa tacgctttat cacttaacac actgattcga tttgcattta aatatgcttt
    14281 cattgtaaat aattgatctt ctgcactctt taattcttct ggaaaataaa tgtcattatc
    14341 ttttagtaat gctgttctat agattttagt cgggcttaaa gtatagataa ttcttgaatt
    14401 taacaaagta acttcaggtg ctgtttcttt aaacatagat tgtggtacac cacgaccatt
    14461 aacacctttc attttaatca ataagacatc tgagtgatgt tcatcaataa atgctgctgc
    14521 atcctttaaa gtctctttgt ttatatagtc atcggagtcc acaaataata caaattcacc
    14581 ttctgcttgt tttaacgcca catttctagg tttacctgga ccaccagaat ttgtttcgag
    14641 ttgacttact ttcaagttca atttattgcg atacttttca actatttgta acgtttgatc
    14701 tgttgaacag tcatcaacta caaccacttc aaattcagtt ttcggaaaat cttgtttcgc
    14761 aaggctatta agtaattctg ttatatactt ttctgaattg tatgttggaa ctattactga
    14821 aaatttcatc attatacctc tcccactttg actactatat aaacttagct accaaataaa
    14881 tttctgacta aacgctcact tgatcggcca tcttgatatt taaaatgttt atctaagaat
    14941 ggaatgactt tttctccttc ataatcttca ttgtccaagg cgtccattaa tgcgtcaaat
    15001 gattgcacaa ttttacctgg aacaaatgat tcatatggtt cataaaaatc acgcgtcgta
    15061 atataatctt ctaaatcaaa tgcatagaaa atcattggct ttttaaatac tgcatattca
    15121 tatattaaag atgaatagtc actaattaat aaatctgtta tgaacagtat atcattaact
    15181 tctctaaagt cagaaacgtc aacaaaatat tgtttatgtt tgtctgcaat attaagtcta
    15241 tttttcacaa atggatgcat tttaaataat acaaccgcgt tatttttttc gcaatatctt
    15301 gctaaacgtt caaaatcaat tttgaaaaat gggtaatgtg ctgtaccatg accactacct
    15361 ctaaatgttg gtgcgaaaag aatgactttc ttacctttaa taattggtaa ttcatcttcc
    15421 atctcttgtt tgatctgtgt cgcataagct tcatcaaata gtacatcagt acgtggaaca
    15481 cctgtaggca ctacattttt ctctttaata ccaaatgctt cagcgtagaa tggaatatcg
    15541 gtttcagatg atacataagc ttttgtatag ctacgatgat ttaatgaatc aataaatggt
    15601 ccaccctttt taccagtacg actaaagcca actgttttaa aggcaccaac ggcatgccat
    15661 acttgaataa cttcttgaga acgtctaaaa cgcactgtat aaatcaatgg gtgaaagtca
    15721 tcaacaaaga tgtagtctgc cttcccaagt aaatatggca atctaaactt gtcgatgatg
    15781 ccacgtctat ctgtaatatt cgctttaaaa acagtgtgaa tatcatactt tttatctaaa
    15841 ttttgacgta acatttcgtt atagatgtat tcaaagtttc cagacatcgt tggtctagag
    15901 tctgatgtga acaacaccgt attccctttt ttcaagtgga aaaatttcgt cgtattaaat
    15961 atcgctttaa aaataaattg tcttgtatta aatgattgtt tgcggaaata cttacgtaat
    16021 tctttatatt tacgaacgat ataaatactt ttaacttccg gagtcgttac aacaacatca
    16081 aggacaaatt cattaacatc gctagaaatt tcaggtgtaa cagtataaac cgttttcttc
    16141 gaaatgccgc cttttctaaa ttcttttagg taagtctgca ataagaaatt gattttacca
    16201 ttttgtgttt ctaattcgtt gtattcttct tcttgttctg gctttagatt ttgatatgca
    16261 tcattaatca catctgggtt taactgtgca atataatcaa gttcttgctc attcactaat
    16321 aagtacttat cttcaggtaa gtaataacca ttatctaaga tagctacatt gaaacgacaa
    16381 acgaattgat tcccatctat tttgacatca ttcgccttca ttgtacgtgt ctcagttaaa
    16441 tttcttaata caaaattact atcttctaaa tctaggtttt cactatgtcc ttcaacgaat
    16501 aactgaacac gttcccaata gattttatct atatatatct tacttttaac caacgttaat
    16561 tcatcctttt ctatttacat aatccatttt aatactgttt taccccaaga tgtagacagg
    16621 tctgcttcaa aagcttctgt aagatcatta attgttgcaa tttcaaattc ttgacctttt
    16681 aacaacgcta atttatctac aatatctggg tattgaatgt ataagtctac aacatcttgg
    16741 aaatcttttg aaccacttcg actactacca atcaacgtta gtcctttttc caatactaga
    16801 cgtgtattaa cttctactgg gaactcactt acacctaaca gtgcaatgct tccttctggt
    16861 gaaatgtaat cgatcatttg atttatggct gattgactac cgcgaccacc cacacactca
    16921 aatgcatgat caaatgttaa gccttcaggt attttattaa taaagaagac atcatcaaca
    16981 aatgagaagt gactcaattt ataatctgtt ttaccaaaga catatatttt agactctgga
    17041 tataatttac gtaataaaat ggctgtaatg taacctaagt taccatcacc ccaaatacca
    17101 aatgtatttt tatttgaaat agatttcttt tcaaaacgac gaatagcatg caaacttact
    17161 gttacaagct ctgtatatga aataatactt aaatcaatat catcaggtag tggtacagct
    17221 ctatcatgat ttagcaacac aaaatcttgc ataaacccat catgtccact tgatctgaag
    17281 tagctcgatt ttaaatagtt ttcagcaatg acatcgtctt tttctgtcgg cgtattcggt
    17341 accataacta cttttgtacc tttattaaac acacctttac tgtcaaatac gacctcacca
    17401 acaccttcat gaattaaaga cattggcaat ttctgagata agacattctc atcacggcta
    17461 ccagtataat atctttgatc ggcagcacaa attgacatat ataaaggacg tacaatgaca
    17521 tagtcactgt aaatatctac gttgttatac gtaacttcaa attgtctagg tgcaactaat
    17581 tgatatactt gattaatcat cggcaatacc acctcgaata atagcattcg ctacttttaa
    17641 atcgtaaggt gttgttactt taatgttata taactcacct tttacaagtc gaaccggttt
    17701 gtttgtttct acaataatct tacaagcatc agataaaata ctcttttgct catcactcaa
    17761 ctgtgcatag ctttctttta ataaattaat attaaacgat tgaggtgttt gaccttggta
    17821 catttcatta cgcactggaa ttgcatcaat cgtttgatta tctttagatg taacaatcgt
    17881 atctatagca tcaatcactg tatctactgc accgtactct aaagcagctt gaatattttc
    17941 tttaataata cgatgcgtta aaaatggtct aactgcatca tgtgtcacaa tgacatcgtc
    18001 atcgttaata ccatttgttg attcaatatg tttaacgata ttcataattg tatcgttacg
    18061 atcgctacca ccttgaatga cttcaattct ttcatcagaa attttgaatt ttctaagtgt
    18121 atctttcgta tgcgtcatcc attgttgtgg cgtcgcgata ataatttttt caaaatcatt
    18181 aattaaaata aatttttcta atgtatggat taaaatcggt ttgttgtcta aatctaaaaa
    18241 ttgtttaggt aaaggtacgt tacccattct tgagcctata cctccagcta gaataccagc
    18301 gtatttcata aaatacttcc t
//
LOCUS       DALAGF010000050        17794 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.050, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000050 DALAGF010000000
VERSION     DALAGF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17794
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.050"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            109..825
                     /locus_tag="O2692_001548"
     CDS             109..825
                     /locus_tag="O2692_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCZ4299181.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            825..1298
                     /locus_tag="O2692_001549"
     CDS             825..1298
                     /locus_tag="O2692_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCZ4299182.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTFSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            1390..1506
                     /locus_tag="O2692_001550"
     CDS             1390..1506
                     /locus_tag="O2692_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299183.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
     gene            1651..2295
                     /locus_tag="O2692_001551"
     CDS             1651..2295
                     /locus_tag="O2692_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCZ4299184.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            2411..3277
                     /locus_tag="O2692_001552"
     CDS             2411..3277
                     /locus_tag="O2692_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ4299185.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKEWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            3547..4767
                     /locus_tag="O2692_001553"
     CDS             3547..4767
                     /locus_tag="O2692_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HCZ4299186.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            4764..5252
                     /locus_tag="O2692_001554"
     CDS             4764..5252
                     /locus_tag="O2692_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HCZ4299187.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(5351..6040)
                     /locus_tag="O2692_001555"
     CDS             complement(5351..6040)
                     /locus_tag="O2692_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HCZ4299188.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
     gene            6167..6610
                     /locus_tag="O2692_001556"
     CDS             6167..6610
                     /locus_tag="O2692_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4299189.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQVESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISNTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            6677..7072
                     /locus_tag="O2692_001557"
     CDS             6677..7072
                     /locus_tag="O2692_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299190.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSADKDQKANDKHQS"
     gene            7210..7509
                     /locus_tag="O2692_001558"
     CDS             7210..7509
                     /locus_tag="O2692_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299191.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            7589..8131
                     /locus_tag="O2692_001559"
     CDS             7589..8131
                     /locus_tag="O2692_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4299192.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(8227..8793)
                     /locus_tag="O2692_001560"
     CDS             complement(8227..8793)
                     /locus_tag="O2692_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCZ4299193.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(8795..9253)
                     /locus_tag="O2692_001561"
     CDS             complement(8795..9253)
                     /locus_tag="O2692_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCZ4299194.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            complement(9256..9939)
                     /locus_tag="O2692_001562"
     CDS             complement(9256..9939)
                     /locus_tag="O2692_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299195.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            complement(10111..10986)
                     /locus_tag="O2692_001563"
     CDS             complement(10111..10986)
                     /locus_tag="O2692_001563"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCZ4299196.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QISYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
     gene            11199..12830
                     /locus_tag="O2692_001564"
     CDS             11199..12830
                     /locus_tag="O2692_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCZ4299197.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGIIVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNYNNEFLLKYEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            12827..14500
                     /locus_tag="O2692_001565"
     CDS             12827..14500
                     /locus_tag="O2692_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HCZ4299198.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSVRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAITRMLLKDAASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(14627..15070)
                     /gene="mgrA"
                     /locus_tag="O2692_001566"
     CDS             complement(14627..15070)
                     /gene="mgrA"
                     /locus_tag="O2692_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HCZ4299199.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
     gene            15297..16223
                     /locus_tag="O2692_001567"
     CDS             15297..16223
                     /locus_tag="O2692_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCZ4299200.1"
                     /translation="MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIM
                     NEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGI
                     AHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMII
                     INKIDLITDDSLEKIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHH
                     GIKSMTYTFTGPIDRHLFYQFIMKLPESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYG
                     IIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
     gene            16326..17234
                     /locus_tag="O2692_001568"
     CDS             16326..17234
                     /locus_tag="O2692_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCZ4299201.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIVQDIP"
     gene            17269..17556
                     /locus_tag="O2692_001569"
     CDS             17269..17556
                     /locus_tag="O2692_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299202.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFVKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
BASE COUNT         6169 a         2486 c         3090 g         6049 t
ORIGIN      
        1 tgaagaaatc gtagttcaat aatgttaatt gtgaaaatgt atataaacat aaaaaaatca
       61 tgtataatat atgttgttaa ttaaacagtt cgaaagcgag atgacattat gggacgtaaa
      121 tggaataaca ttaaagaaaa aaaggcccaa aaagataaaa acacaagtag aatatatgcg
      181 aaatttggta aggagattta tgttgcagca aaatctggtg aacccaatcc agaatctaac
      241 caagctttaa ggttggtgct tgaacgcgct aagacatatt cagtgccgaa tcatattatt
      301 gaaaaagcaa tagataaagc taagggtgct ggagacgaaa actttgatca cctaagatat
      361 gaaggatttg gcccaagcgg atcaatgcta attgttgatg cgttaacaaa taatgtaaat
      421 cgtactgcct ctgatgtgcg agctgctttt ggtaaaaacg gcggtaatat gggtgtatct
      481 ggatcagttg cttatatgtt tgatcatgtg gcaacatttg gtattgaagg aaagtctgtt
      541 gacgaaatac ttgaaacatt aatggaacaa gatgtagatg taaatgatgt gattgacgat
      601 aatggattga caatagtcta tgctgaacca gatcaatttg cagtcgttca agatgcgctt
      661 cgtgcagcag gtgttgaaga atttaaagtt gctgaatttg aaatgttacc tcaaacagat
      721 attgaacttt ctgaagcgga ccaagtaaca tttgaaaaat taatcgatgc attagaagat
      781 ttagaagatg tacaaaacgt attccataat gtggatttga aataatgaaa tcagcagaac
      841 aatggattga tgaattgcaa cttgaatcac atcctgaagg tggtttctat agagagacaa
      901 ttcgagaagt attgaaagat ggacgcagag cgccgtttag tagtatttat tttttactta
      961 cagatgacaa tatttcgcat tttcatcgaa ttgatgctga tgaagtatgg tactatcatg
     1021 ctggcgattc tctaacaatt catatgataa atccggatgg ggaatatacg actgcaacat
     1081 tgggtactga tatccaaaat ggagatgtat tgcaatatgt agtgcctaaa ggaacaattt
     1141 ttgcttcttc aatcgaaata tcaaatactt ttagtttagt aggttgtatg tgtcaaccgg
     1201 catttgagtt taagcagttt gaattgttta agcaatctga attaattacg caatatccgc
     1261 atcttaaatc agtaatcgaa aaatatgctt taaaataaaa gtgatcaatg aagtggtttg
     1321 aaggttgtta ataaaccttt gagtcacttc atttttatat gtattcttga ttgaatcaga
     1381 atagatttga tgcttcagct gtttttaatg aaaatagcat taaatgattt tgaaaacgat
     1441 aagagtgtgt tatttatatt tttgaaaaat cacttttatg aagaaatgtg tgtactatct
     1501 aattaatatt gtctatttta agtaaattga agcgagttgt agtaaaataa taataaatat
     1561 tttcatatga ttaacaaaaa ctataaaact gtatcatgac atgacatcat ttatgtagtt
     1621 ttatataata atgacaagga gttggtgact gtgaaagtaa agtatataga taaacgtcac
     1681 tggcgtcgcc taattgatag ggaatacaca gaggtaaaag ttaataataa taggtttaag
     1741 ggtattatag gcttagtcac gatgaaaaag gttcgtgatc ctttagaggt gacggtagtt
     1801 ggacaaaata tcattgtcgc agatgacaat tataaatggt tgcaaatact acctgaaaag
     1861 aaacgttata gtataactgt aatgtttgat aataaaggca atccattaga atattatttt
     1921 gatataaata tcaaaaatat aacgcaaaaa ggtaatgcgc gtacagtaga tttatgttta
     1981 gatgttttag cattaccaag tggtgaatat gagttggtag atgaagatga tttaatgttt
     2041 gcattagaaa gtgagcaaat tacaaaaaag caatttcatg aagcatatat gattgcacat
     2101 caaattatgg cagagttaga aaatgatttt aaaggattcc aaaagaaaat catgtactgc
     2161 tttaataaaa ttaatgcaaa ggctcaaaaa aatcatcaaa agccacaaaa taaaactaat
     2221 attgaaaaaa gcaaacaaat aaagcctaag caatataatc aaactaaaaa tcaccaacaa
     2281 caaaagaaaa actaagtaat tcaagctgca gccataccaa taaaattggt gaaattattc
     2341 gttagtaatg aactcatgaa tgcttgtagc catgaaagtt caataattga ataatttatg
     2401 gggggaaatt atgaagattg aagactatcg tttactaata acattagacg aaacgaaaac
     2461 gttacgtaaa gcggctgaaa ttttatatat atctcaacct gctgttacac aaagactaaa
     2521 agctattgaa aatgcttttg gagtagatat ttttatcaga acaaaaaaac aattgattac
     2581 aacaactgaa ggaacaatga ttattgagca tgctcgtgac atgttgaaaa gagagcgatt
     2641 attttttgac aaaatgcagg cacatattgg tgaagtgaat ggaacaatat caatcgggtg
     2701 ttcttctttg attggacaaa ccttacttcc tgaagttttg agcctatata atgcccaatt
     2761 tcctaatgtt gaaatacaag tgcaagttgg ttcaactgaa caaattaaag caaatcatag
     2821 agattatcat gttatgataa ctcgtggaaa taaggtaatg aatttagcta acacacattt
     2881 atttaatgat gatcattatt ttatttttcc aaaaaataga cgagatgatg ttacaaagtt
     2941 accatttata gagtttcaag ctgatccgat ttatataaat caaataaaag aatggtataa
     3001 cgataattta gaacaagatt accatgcaac tattacagtg gatcaagtag caacttgcaa
     3061 agaaatgttg attagtggtg taggtgttac aatcttgcca gaaattatga tgaaaaatat
     3121 cagcaaagaa caatttgagt ttgaaaaagt agaaattgat aatgaaccgc tgattcgttc
     3181 gacatttatg agttatgatc cgagcatgtt gcaattgcca caagttgatt cttttgtaaa
     3241 tctcatggcg agctttgttg aacaaccaaa ggcgtagttt tagactaatt taaggtttgt
     3301 atttaatttt aaactattcg gttaaattga acgtagttgg ttgctaatgc accaacagca
     3361 aaagagcccc taattaataa attaaaaggg gacaaaggaa tacagttggt tgctaatgca
     3421 ccaactgcat aagagcccct aattaataaa ttaaaagggg acaaaggaat acagttggat
     3481 gctaacgcac caactgcata agagccccta attaataaat taaaaggggc tctaagaagc
     3541 ggggttatgg atatgtttgc agcgttatta caaataaaga attataaact ctttgttgct
     3601 aatatgtttc tactaggtat gggtattgcg gttacggtcc catatcttgt tctttttgca
     3661 actaaagatt taggtatgac aacaaatcag tatggattac ttctagcatc tgcagcgatt
     3721 agccagttta cagtaaattc aattattgct agattttcgg atacgcatca ctttaataga
     3781 aaaattatta ttattctcgc attattaatg ggtgcgcttg gtttttcaat atactttttt
     3841 gtagatacaa tctggttatt catattacta tatgcgattt tccaaggatt atttgcacca
     3901 gcaatgcccc aactttacgc atctgctaga gaatctatca atgtttcaag ctctaaagat
     3961 agagctcaat ttgccaacac agtattacgt tcaatgttct cattgggctt tttatttggt
     4021 ccatttattg gtgcccaatt aatcggatta aaaggctatg ctggattgtt tggtggaaca
     4081 ataagtatca ttttatttac tttagtactt caagtgtttt tctataagga tttaaacatt
     4141 aaacacccta ttagtacgca acaacatgtt gaaaaaattg ctcctaatat gtttaaagac
     4201 aaaacgcttt tattaccatt tattgcattt attttattac acattggaca atggatgtat
     4261 acgatgaata tgcctttatt tgttactgat tatttaaaag aaaatgaaca acatgtcggt
     4321 tatttagcta gtttatgtgc tggtttagaa gtgccattta tgatcattct tggcgtttta
     4381 tcatctagat tacagactcg aacattgttg atttatggag cgatttttgg tggtttattc
     4441 tacttcagca ttggggtatt taaaaacttc tatatgatgt tagcaggaca ggtgttttta
     4501 gctattttct tagcggttct tttaggaatt ggtattagtt atttccaaga tatcttacca
     4561 gattttccag gatacgcttc aacactattt tctaatgcaa tggttattgg acagttaggc
     4621 ggtaacctat taggtggtgc tatgagtcac tgggtaggtt tggaaaatgt attttttgta
     4681 tcagcagcat caatcatgtt aggtatgata cttatattct ttactaaaaa tcaaaaaatt
     4741 acaaaagagg atgtgatatc aacatgacaa ttattttatg gctacttatc atcgctgcct
     4801 tcatgttagc atttgttggg ttgattaagc cgattattcc ttctgtttta gtattatggg
     4861 ttggcttttt aatctatcag tttggctttc ataatcagca tttatcatgg gtgttttatg
     4921 tatctatggc attgctaaca atattaattt tatgtgccga ctttttagct aataaatatt
     4981 ttgtgaatcg cttcggtggt tctaagtttg gagagtatgc agctttaatt ggtgtggtta
     5041 ttggatgttt tgttttaccg ccatttggaa ttattattat accttttatt ttggtattca
     5101 tagttgaatt aatacaaggc tattcatttg aaagagcagt taaagtaagt ataggttcaa
     5161 tcgtagcatt tttaacaagt agtatagctc aagcaatcat tatgtttata atgattgtat
     5221 ggttctttat agatgcttta ttgattaatt aataaaaagc ttattgcaaa atatgttttt
     5281 cggtaactgt aatttagtga ttttatcatt aacagtacca aattcgcata tgatgtaata
     5341 agctttttgt ttataaaaat gtatgagaat gttttttcga aatatttctt tcaatgcgta
     5401 atccaatagg cataactata aatgaaaata taataaatac ccaattgctt acctgaatat
     5461 atggtccaaa ggcaagtaat gattgtggaa taaagaatac atagaaaaat cctacaatta
     5521 gtagaatgat tgctaaatag gtgaatatcc atatccaatt tgaattgtta aaacactgca
     5581 gttgtattgt tttaaaacta aaccatataa ataaaaagac aataaagaat ccaacgacta
     5641 ctgaaaacgg gaatgaaaca aaatataaat tacttccatt tttttccatg aaaaatccta
     5701 aaaatccttt gagaaaacta acaatcccaa ttaatagaac gatgtgttga tagatatatt
     5761 taaaaatatt tttaaatgtt tcattaggca tcgcttttag ttcttttatt gcatgtgctt
     5821 ttgggtcgtg attgaaaaaa tctaaggcta ataaaccatg ttgttctgcg cttaataatt
     5881 gtttgagtat acggttaata attaactctg tatcatgagg gttgacgcga aagtcagagc
     5941 gcatataagt catataattc tcgaagattt ctctatcagt attgcttaat cttaatgatt
     6001 taacattatt ttcttttgtt aattgcgcag tacttttcat tgttacttaa gcgctccttt
     6061 aaaaatgttt aattccaaat taaaatggaa atgattttat agtattaata aggtcaatca
     6121 tatcatatta aacgcataaa tataacgatt aatattggag aggaaaatga ggacacttaa
     6181 taaagatgaa cataattata tcaagcaaat agctaatata catgagacat tattgtcgca
     6241 agtagaatcc aactataaat gtactaaact gagtattgct cttaggtacg agatgatatg
     6301 ttcaagatta gaacatacaa atgataaaat ttatatatat gaaaatgaag gtcaattaat
     6361 agcgtttatt tggggacatt ttagtaatga aaaaagtatg gttaacattg aactgctata
     6421 tgttgaacca caatttcgca aactgggaat agctacgcaa ctgaagattg cgcttgaaaa
     6481 atgggcaaaa actatgaatg caaagcgaat aagcaataca attcataaaa ataatttgcc
     6541 aatgatatct ttgaataaag atttaggtta tcaagtgagt catgtgaaaa tgtataaaga
     6601 tattgattag aattaggatt atgttgctaa ttcatgttaa aattaaaaaa gatttaatga
     6661 cgttaaggag ttttatatga agaaattaat catcagtatt atggcggtca tgctattttt
     6721 aacaggttgt ggtaaaagtc aagagaaagc cactctggaa aaggatatcg ataatttaca
     6781 aaaagaaaat aaagaattaa aagacaaaaa agaaaagctt caacaagaaa aagaaaaatt
     6841 agcagataag caaaaagacc ttgaaaaaga agtgaaagat ttaaaacctt caaaagaaga
     6901 taacaaggat gataaaaaag acgaagacaa aaataaagac aaagataaag ataaagaggc
     6961 atcacaagat aagcaatcaa aagatcaaac taagtcatcg gataaagata atcacaaaaa
     7021 gcctacatca gcagataaag atcaaaaagc taatgacaaa caccaatcat aatcgaattg
     7081 cttacttgtt atagatgaaa ggtacagcgt tttaaacctt attttaaggg tatgtattaa
     7141 ttaaaatgtg gtcatgattg aaacagaacg taaaaataga caacgtaatt aataaaggag
     7201 agaaacggca tgcatgaaca agattttaga attttagagg gtcaagatat tactttgcca
     7261 gaattaggta gagaattaga aaatattaca ggacatacga ttgctgattc tactggcgaa
     7321 attaagcgtg taattgcaca tttaccaaac tttgagtccg atacagatac ttttgttgct
     7381 acatatcgtt taaaccatca acaagatttt atagatgcaa cttttactgc gctgaaatca
     7441 gatagagcac gtttaaaaga agtgccagtt catgttgaac ttataagtta tatttctaaa
     7501 tcaaaataaa ctgctatcta aaacgcaaag ttgatcaaaa tatcgatttt gtgtttttta
     7561 ttgagaaatt atataggagt gtcaatcgat gatttattgt gaaacagagc gtttaatatt
     7621 aagagactgg catgaagatg atctgttacc ttttcaaaaa atgaatgcga attatgacgt
     7681 acgtaaatat tttccaagtt tattgagtta tcgtcgttca gaattagata tgagaactat
     7741 ggatgcggtt attaaagatt atggcattgg attatttgct gtagaagata aagagtccca
     7801 tcaatggata ggctttatag gtttgaatta tattccagaa acaagcgatt atccatttaa
     7861 agaattaccg ctttatgaaa taggttggcg cttgttgcca gaattttggg gaaaaggatt
     7921 agcaactgaa ggcgcaaagg caacattgaa gttagcagaa gaacatcaaa tatacgatgt
     7981 ctatagtttt acagcagaag caaataaagc ttcacaacgt gtaatggaaa aaattggcat
     8041 gacagtgtat gatcatttcg aattacccaa tctaagtaag tatcatttat taaaaaggca
     8101 agtgcgctat tacattaatc ttcgaaagtg aaaaatttat acataagcgt aacaaacacc
     8161 cctaacattg tttagctgat gataacaata ttaggggtgt ttgtttattt tttaacctta
     8221 gaatgattaa tcgtatgaac gagtacccag aggtttgaaa tttaatattg attcaattaa
     8281 tgattcctta gtgtcgcata acggtgcaag agcacgatac ttaggatcaa taaaaccttc
     8341 ctcaatcata tggtcaatca ttgtttgtag tggattgaaa aagccattaa tattataaat
     8401 ggcaataggc ttttcatgga tacctatttg agcccaacta tacatttcga aaaattcttc
     8461 tagtgaacct gcgccaccag gagccatgac aaatgcatct gcaagttctg ccattttatt
     8521 tttacgttca tgcatagaat caactaaaat taattcagtt aaacgttggc ttgtgatttc
     8581 atgttcatct aacattttag gcatgacgcc aatagctttg ccgccatgat ctaatacacc
     8641 atcttgaatg gcacccataa tgccaattga ccctgcacca aatactaatt cataaccttg
     8701 ttcagcaaaa tatttaccta aatcgtatgc tttttgtaca tatgaagggt catgaccttt
     8761 gcttgcacca caataaactg cgattcgttt catgttaatc cagctcctta attcgatgaa
     8821 tgacttttaa tagtgattgt tcaaacactt tttgatcttg ctttgtaaaa ggtgggggac
     8881 ctttgtggcg accaccttgt tttctaattt gtgcattcat atatcgttta tctaatagtt
     8941 gttgaatatt tttggaattg tatatcttcc cattatgatg catgacaatt aagactttgt
     9001 cgactaataa acttgcgagt ccataatctt gagtgactac gatatcatcc ttcgttgata
     9061 attgaacaat tttgtaatca actgcatctg gtccatcatc aacatataat gttgatacat
     9121 gtggaggata taattggttc gaaaaatggc tgaagctccg aataattgtc acaaaaatgc
     9181 ctgtctcagt tgttaaatct ataatagaat caacaacagg acaagcatct ccatcaataa
     9241 taatatgtgt cacaattatg cctctgtatt gttttcttta ttttgttgag aggcgctttt
     9301 ggcaacataa tctttatatt ttttaaatga cttgatgcgt gctttatcag cttcttgttg
     9361 gcgtttttgt tcttctttgt gtcgtttttc aatatttttt tgtaactttt tattcatttt
     9421 agcgatttct ttgcgatttt tttcagctag tttatcgcct tttttctcag ttttctcatc
     9481 taatttatta ggtgttaagc ctgctttttc ttcgtatttt tgtgattttt tcatatcttt
     9541 aatacgttgt atttcattct tttcgcgggc tttttgctct tctttatgac gcttttcgat
     9601 atttttttga agtattttat tcattttatc agcgtcttta cgattttgtt tagctaattt
     9661 ttcgcctttt ttctcaatat aggcaggatc atgttctcta gcaaactttt taagttcacg
     9721 tttattttca aaatcttgtt ttttatcgcc gacatattct ttaacatcac tcgctgtgtt
     9781 actgattgct gcagatgttt ttgaagcaac tttacttgta gcatctgtaa ctttttgtac
     9841 gtccggatgt tgtttgatac gtttacgttc aacaattaac ggtaccaata caattggtaa
     9901 tacattaatc ataaatttga tgactttttt cttatccata gatcttgcct ccataattac
     9961 tttattaatt ttacataccc tatgatacat caatataaac gatgatagta gtgaatcact
    10021 attaagtatt tcagatgttt tttaaaagaa gacaataaaa actgccaatc aagtgattcc
    10081 ttaattgaca gtctatattt taaaaggaaa ttaaatacct ttaccaatgc caaatccgaa
    10141 gtaaagtata gcaataaaga ttactaatac aattctgtaa atggcaaatg gaattagttt
    10201 gattttgtta attagatgca agaatgtttt gattgcaatt agtccaacag taaatgcagc
    10261 taaaaagcct aaaatataaa aaggtatatc agcaatctga atatcttgat aatgttttaa
    10321 taaagataaa ccactagctg ctaacataat tggaacagcc ataataaatg taaagtccga
    10381 tgctgcttta tgatttaatt tcattaatac cccagttgaa attgttgagc ctgaacggct
    10441 gaaaccaggc cacatagcta ctgcttgaga aataccaatt acaaatgctt ggaaataact
    10501 gatttgatct actgtttgtg ggtttttaac tttagctgag tatttatcag caataatcat
    10561 atagatagca cctacgaata agccaatcat aacagttggc acactaaata aatgttcttc
    10621 gatgaaatca tcaaatagta agcctaaaat acctgctggc accataccca ctaatacatg
    10681 taataaattt aaacgtcttg gctttgaacg tctttgttga tcgttatctc cttcaacatg
    10741 tttgtgttta ccaatatgta aaatctctaa gaagcgttcg cggaacaccc atgctgctgc
    10801 aaagacggat cctaattgga tgacgatttt aaatgtaaat gctgactgag aacctaaaaa
    10861 ttcagatgat tttaaccaca tatcatcaac taggatcata tgtccagtag aggaaacagg
    10921 tgcaaattct gttaatcctt cgacgacccc taagataata ccttttatta attcaatgat
    10981 aaacataatg tacccacttt cattactcaa tttaatttat ttaaatatca aaattaccat
    11041 atcatgatag catattcatt taaagacatg ctagttataa tactagacta aagatgtata
    11101 tattcatttt cttttacatg taaaactaca atattttatt gagctattta atttgatttt
    11161 aaggaaaacc ttttataata ggtttaggtg atataattgt gaaaaaatta acaacaatac
    11221 tgtttcaata taaaattttt ccggtactca tgttcttggt cagtactggt ctcggcataa
    11281 tcgttataac gcaaaatatt ttaatagcag attttttagc taaaattata agacatcaat
    11341 ttcaaggttt atggattgta ttatttattt tattaggtgt tttactttta agagcaactg
    11401 tgcaatttct aaatcaatgg ttaggtgata cattagcatt taaagttaag catatgctta
    11461 gacagcgggt tatttataaa aataatggtc atccaatcgg tgaacaaatg actatactca
    11521 cagaaaacat tgatggtcta gcaccttttt ataagagtta tttgcctcaa gtgttcaaat
    11581 caatgatggt tccgctcatc ataatcattg caatgttttt catccatttc aataccgcat
    11641 taattatgtt aataactgca ccatttattc ctttgtttta tattattttc ggtttgaaaa
    11701 cgcgagatga gtcaaaagat caaatgactt atttgaatca atttagtcaa cggtttttaa
    11761 atattgctaa aggtttagtg acgttaaagc tatttaatcg tacagagcaa acagagaagc
    11821 atatttacga cgatagtact cagtttagaa ctttaacaat gcgcatttta cgcagtgctt
    11881 ttttatcggg attaatgctc gaatttataa gtatgttagg tattggattg gttgcattgg
    11941 aagcaacgct aagcttagta gtatttcata atattgattt taaaactgcg gcaattgcga
    12001 ttattttagc gcctgaattt tataatgcaa ttaaggactt agggcaagcg ttccatactg
    12061 gaaaacaaag tgaaggtgcc agtgacgttg tgtttgagtt tttagaacaa ccgaactata
    12121 ataatgaatt tctattaaag tatgaggaaa accaaaagcc atttattcag ttaacagaca
    12181 tatcatttcg atatgatgat tctgatagat tggtattaaa tgatttaaat ttggaaatat
    12241 ttaaaggtga tcaaattgca cttgtaggtc caagcggggc aggtaaatcc actttgacac
    12301 atcttattgc aggtgtttat cagccaacaa taggtactat aagtacaaac cagcgtgatt
    12361 taaatatagg aatacttagt caacagccat atattttcag tgcttctata aaagagaata
    12421 ttacgatgtt taaagatata gaaaataata ctattgaaga agtgctagac gaagtaggtt
    12481 tattagacaa agtgcaatct ttcacaaaag gcattaacac aataataggt gaaggaggcg
    12541 aaatgttatc tggtggacag atgagacgca tagaactttg ccgtctttta gttatgaagc
    12601 cagatctcgt tatatttgat gagcctgcaa ctggtttaga tattcaaaca gaacacatga
    12661 ttcagaacgt tctgtttcaa cattttaaag atacaacgat gattgtcatt gcacatagag
    12721 ataatacaat tcgccattta caacgacgct tgtatataga aaatggaaga ctgattgctg
    12781 atgatcgcaa tatttcagta aatataacag aaaatggtga tgacttatga aaacacgact
    12841 aaaatttcaa gtagataagg atttattgtt agctatagtt gttggtgttt gtggaagttt
    12901 agttgcgctc gccatgtttt tcttaagtgg ttatatggtg acacaaagtg cacttggtgc
    12961 gccactatac gctctgatga ttttagtcgt tacagtaaaa ttgtttgggt ttttaagagc
    13021 tattactcga tacgtagagc gccttatttc tcataaagct acatttacaa tgctacgtga
    13081 tattcgggta cagtttttcg gtaaattagt aaatgtcatt cctaatgttt accgtaaact
    13141 gagttctagt gatttaattt cacgtatgat tagtcgtgtt gaggcattac aaaatatata
    13201 tttacgtgtt tattatccac cagtcgtcat cggtttgaca gcgctagtta cagtcatagt
    13261 tttggcgttc atttcaatcg gccatgcgct attgattatg gttagcatgt tgttcacttt
    13321 actcattgtt ccttggttaa gctcaaaaaa agcacgtact ttaaagaaac atgcagctaa
    13381 tgaacaggcc cgatttttaa atcattttta tgattataaa gctggtatgg atgaactacg
    13441 tcgatttaat caaattaatc attatcgaga taatttgatg gctaaattaa atcattttga
    13501 taaattacaa cttaaagagc aacgcttttt aacgatttat gattttatat taaatattat
    13561 tgctatgctt tcgatttttg gtagtttagt tctaggatta attcaaatta atgcaggcca
    13621 actaaatatt atttatatga cgagtatagt tttaatggtc ttaactttat ttgaacaagc
    13681 tgtaccaatg acaaatgtcg cgtattataa agcggatact gaccaagcat tgcacgatat
    13741 taatgaagtg atatctgtac cttctactaa tggaaaaaaa cgtcttaatg ataagtatga
    13801 tgcaacgaac atttatgaag ttaaggatgc tagttttaag tattggaatc agcaaacgta
    13861 tgtgttgtcg gatattaatt ttaatgttaa tagaggcgaa aagattgcga ttgtgggtcc
    13921 ttctggttca ggaaaaagta cattactaca aattatggca gggttatatc aattagatag
    13981 tggctctgtt cgtttcgaaa atatggatat gtttgaaata gatgacaaag ataagtttga
    14041 atcgttaaat gtcttgctac aatctcaaca attatttgat ggtacaatac gtcaaaattt
    14101 atttaccgat gaaaaagatg aagcggtgca agcaatattt aagcaattag atttagaaca
    14161 tttggcacta gaacgtcaaa ttgacttaga tggtcataca ttatctggcg gagaaattca
    14221 gcgtttagcg attacgagga tgttattaaa agatgctgca tcaacatgga ttttagatga
    14281 accaacaact gcattagata aacaaaatag tttaaaagtt atggatttaa ttgaagcaca
    14341 tgcagaaaca ttaattgttg ctacacacga tttaacttta ttgtcacgtt ttgagaccat
    14401 cattgtgatg ataaatggta aaatagttga aaagggaaac tatcaacaat tactagctaa
    14461 tcaaggtgct ttatggaata tgattcaata taatgcataa aaaaactgct tgataggcta
    14521 agaaaccttt caagcagttt tttaactaac aagcttacgc gtgctaattg tttttctatt
    14581 cttaataaat tctaaacatt actttaattg tcatgacaaa agttaattat ttttcctttg
    14641 tttcatcaaa tgcatgaatg actttaccta ataagcgatt aagttcttta acttcatctt
    14701 gcgataaaga agaagctgaa gcgactttgt cagatgcatt acttaattct ggtctaatag
    14761 tttcactttt gtcagtcaag tgaataaata cttcacgttg atcgacttcg gaacgttcac
    14821 gcttaattaa gtctacttgt tccattcgtt ttaataatgg tgatactgta ccagtatcga
    14881 gtgctaattc agttacgact ttcttgacgt ttacaggaga ttcatcccat aaaattgtta
    14941 agacaagaaa ttgtgggtat gttagattgt acttcttaaa aactttgtta gagtagtagc
    15001 gattaacttg tctttgagca ttgtacaaac taaagcatag ctgttctttt aaattatgtt
    15061 gatcagacat taaagttctc ctccagacat actatccgtt tttttctctt ttcggattgg
    15121 taatcattaa aaagttgatt gtttattaat tcacaacttt ctttgattca atgccatgct
    15181 aaaattaaag tatgtttaaa gtttagaaga tatttttgat taaatcaagc aaaaagataa
    15241 tttaatatat atgttatcat ttttaaaaat aactgtaata gaaaagagaa tataaaatga
    15301 aaaataataa agatgaaaaa ataagaatat ccataattaa cggatttttg ggtagtggta
    15361 aaaccacgtt actgacacat tatattagtg aattattaaa aaatgatgag aaaattaaaa
    15421 tcatcatgaa tgaattcggt acttttgata ttgatagcaa tagtatttca aatgaaattg
    15481 aagtccattc attgattaat ggttgtgttt gttgcgatct taaacaagaa cttgtctatg
    15541 aactaaaagc cattgcttta aaaggggacg ttaatcatgt catcatagaa gcgacaggca
    15601 ttgcgcatcc tttggaatta ctagttgcat gtcaagatcc gcaaatcgtt aatttctttg
    15661 aaaagccgat tatttatggt gtattagatg cgactcgatt tttagaacgt catcaatata
    15721 ccgaaaatac agtttcgctg atggaagatc agttgaaact aagtgacatg attattatta
    15781 ataaaattga tcttataact gatgacagtc ttgagaaaat tgataagcaa ttaggtatga
    15841 tttgtgcaag tattccaact tataaaacaa cctatggaaa agtttcgttg gaagaattgg
    15901 acttaactgt taaagacaga gagatatcgt ctcatcatca ccatcatcat gggattaaaa
    15961 gtatgactta cacgtttaca ggtccgattg atcgtcattt gttttatcaa tttataatga
    16021 aattaccgga atctgttcta cgtttgaaag gttatgtgtc atttagagat caaccaaatg
    16081 caatttatga atttcaatat gcatatggtt taccagacta tggaataatt ggcatgcaat
    16141 taccattaac gattgttatt attggtgaaa ctttagatac aaatcacata cgtaatcaat
    16201 tggatatgct acaatttacg taaatcagaa aatgtgttgt ttcaatttaa cggaaatagc
    16261 acattaattt cttaatactt ttattgtgaa tattttgtga cattgaggga tggagtggat
    16321 gagatatgga acaaataatg attaatcact atgttcattt ttctaggctt gtacaaggtt
    16381 tttggcgtgc aaatgaatgg aagatgactg cgaaagagtt aaattatttt ataaatgaat
    16441 tagttgaacg tggaattaca acgatggatc atgctgatat ttatggagat tatcaatgtg
    16501 aatcactgtt tggtaatgct ttggatttat cacccgaatt aagaaataaa attcaaattg
    16561 ttacgaaatg tggtatcatt ttgccttcta agcaatttga ttttacaaat ggacatcgtt
    16621 atgatttgag tagtaagcac atcgtgaaat ctgttgaaca gtcattaatc aatttgaatg
    16681 tagattattt agatagtcta ctcattcatc gtccttcacc attgatggat ccagaacaag
    16741 ttgctgatgc attaactaaa cttgttaaac aaggtaagtt gaagtcattc ggggtgtcga
    16801 attttaatca ttcacaatac caattgttaa atcaatatat tatgaaagaa agactacata
    16861 ttagcatcaa tcaattagaa ttatcgccat atcacgttga tagtttacaa gatggaacaa
    16921 tggattcaat gtatcaaaac catgttcaaa tcatggcttg gagtcctttt gcaggcggta
    16981 aaattttcga caaggaagat attaaagcgc aacgtattat gaaagttgtt caatcaatag
    17041 ctgacaaata tggtgtgagt gacacagctg tgatgatagc gtggttagta aaaataccgc
    17101 atcgtatcat gccgatactt ggaacaagtc agttaaagcg tattgatcaa gcaatcgaag
    17161 ggctacaact taatttagat gatcagtcgt ggtttgacat ttacaccgct attatcgtac
    17221 aagatattcc gtaaacttat ttacttttaa atcataaagg agcatatcat gacaaacgaa
    17281 gataaacgtt tcgaacaatt aagatttgaa cgcaaattta tagttattcc gtatttaatt
    17341 tatgcagtca ttgtattact attaaatatt ttctattctg atttgaaaat aacaatgaca
    17401 ttattcggac ttttctttgc gtataatgta gtcattttgt tcatagcatt tgttaaacat
    17461 tataaacgca cattgttact aagtcttata ttaacagtgc ttagtggcgc ggcattcttt
    17521 ggaattattt atgtttatgg cattaatcat ttttaaacat taaaaagaga ctatctacaa
    17581 taatctcatt tgcattttat ctaactaagt ttaacttttg cttagaaaaa tgatgctatg
    17641 aggatattca gtagatagtt tttttatgat ctaatattta aacttagata tcgttttgta
    17701 attaaccgag ataactcatc acctagcaat attatttaca aaatatttcg ataaaattgt
    17761 gtattttgca taaattaaaa atgtatgttt taaa
//
LOCUS       DALAGF010000051        17695 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.051, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000051 DALAGF010000000
VERSION     DALAGF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17695
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.051"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(247..606)
                     /locus_tag="O2692_001570"
     CDS             complement(247..606)
                     /locus_tag="O2692_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HCZ4299203.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(619..855)
                     /locus_tag="O2692_001571"
     CDS             complement(619..855)
                     /locus_tag="O2692_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HCZ4299204.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            1172..2236
                     /locus_tag="O2692_001572"
     CDS             1172..2236
                     /locus_tag="O2692_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ4299205.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(2295..2618)
                     /locus_tag="O2692_001573"
     CDS             complement(2295..2618)
                     /locus_tag="O2692_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HCZ4299206.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            2719..2961
                     /locus_tag="O2692_001574"
     CDS             2719..2961
                     /locus_tag="O2692_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HCZ4299207.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(3224..4399)
                     /gene="dltD"
                     /locus_tag="O2692_001575"
     CDS             complement(3224..4399)
                     /gene="dltD"
                     /locus_tag="O2692_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCZ4299208.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKEYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(4396..4632)
                     /gene="dltC"
                     /locus_tag="O2692_001576"
     CDS             complement(4396..4632)
                     /gene="dltC"
                     /locus_tag="O2692_001576"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HCZ4299209.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(4650..5864)
                     /gene="dltB"
                     /locus_tag="O2692_001577"
     CDS             complement(4650..5864)
                     /gene="dltB"
                     /locus_tag="O2692_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HCZ4299210.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(5861..7318)
                     /gene="dltA"
                     /locus_tag="O2692_001578"
     CDS             complement(5861..7318)
                     /gene="dltA"
                     /locus_tag="O2692_001578"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCZ4299211.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVNR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(7334..7486)
                     /locus_tag="O2692_001579"
     CDS             complement(7334..7486)
                     /locus_tag="O2692_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCZ4299212.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            complement(7534..7644)
                     /locus_tag="O2692_001580"
     CDS             complement(7534..7644)
                     /locus_tag="O2692_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299213.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            complement(7898..8857)
                     /locus_tag="O2692_001581"
     CDS             complement(7898..8857)
                     /locus_tag="O2692_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HCZ4299214.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            complement(8890..9669)
                     /locus_tag="O2692_001582"
     CDS             complement(8890..9669)
                     /locus_tag="O2692_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCZ4299215.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            complement(9669..10103)
                     /locus_tag="O2692_001583"
     CDS             complement(9669..10103)
                     /locus_tag="O2692_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HCZ4299216.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVGIRKQFTYDYTALD
                     IKIIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            10201..10467
                     /locus_tag="O2692_001584"
     CDS             10201..10467
                     /locus_tag="O2692_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HCZ4299217.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            complement(10546..10929)
                     /locus_tag="O2692_001585"
     CDS             complement(10546..10929)
                     /locus_tag="O2692_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCZ4299218.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(11059..11976)
                     /gene="lipA"
                     /locus_tag="O2692_001586"
     CDS             complement(11059..11976)
                     /gene="lipA"
                     /locus_tag="O2692_001586"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCZ4299219.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            complement(12060..13379)
                     /locus_tag="O2692_001587"
     CDS             complement(12060..13379)
                     /locus_tag="O2692_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCZ4299220.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            complement(13406..14233)
                     /locus_tag="O2692_001588"
     CDS             complement(13406..14233)
                     /locus_tag="O2692_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCZ4299221.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            complement(14246..15094)
                     /locus_tag="O2692_001589"
     CDS             complement(14246..15094)
                     /locus_tag="O2692_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCZ4299222.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            complement(15142..15240)
                     /locus_tag="O2692_001590"
     CDS             complement(15142..15240)
                     /locus_tag="O2692_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299223.1"
                     /translation="MRFYFTNLIKRKHQAEDRRKILIKQKHQTKVR"
     gene            complement(15307..16374)
                     /locus_tag="O2692_001591"
     CDS             complement(15307..16374)
                     /locus_tag="O2692_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HCZ4299224.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPSEQIISRLKAANVKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVITKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELIHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            complement(16388..17428)
                     /locus_tag="O2692_001592"
     CDS             complement(16388..17428)
                     /locus_tag="O2692_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCZ4299225.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
BASE COUNT         5589 a         3200 c         2589 g         6317 t
ORIGIN      
        1 agcaagattc agtcagattg tgaaaaaatt aattaatttt ttcgtagcct gatttcgatt
       61 aaaaatgtat ccgctatcac gatcatttaa aatactattt atagtcagat tcttaatttt
      121 caataagctt tttaataaaa caaaatatct ttacgcaaaa ataggacata atcaaatatg
      181 tccttaatcg ttattgatat caatttgtat cccaaaaatt gggttaacca ttaaaaagtt
      241 attttattag caattttcag gattacctgc aacttttgca gttctaaatg aactaccaca
      301 gccacatgaa gcaattgcat taggattgtc gatttggaaa ccgccaccca ttaatgattg
      361 cttaaaatca atagtcgtac catttaatac gggtgcatct tttttgtcta ctaatacttt
      421 taatccaaag tattctaaga cttcatcatt ttcaccaggc gcttcttctg cacccatacc
      481 gtatgttaaa ccagtgcacc cgccaccatt cactttaatt tttaaatagc catctggcat
      541 ttcatttgct ttaagcatat cttttacttc gtaagcagct gcttctgtta atataactgt
      601 tggcatgata actcctcctt aaaaaatcca agtttctttt atatgtgcat atatattttg
      661 taataattct tccggcgaat caccttcaac aatatcacca tttactaaag catacaaccc
      721 ggctgaacat ataccacaat gtgtcaggca accatactct aacacatcga catctgggtc
      781 attttccagt tgattaaaaa cataatctcc accttttgcc atgttagaga gacaaaattc
      841 tacgatcgga ttcatacttc accttcttat ttcatttgtt acaatattat agcattttaa
      901 aactggtatt ttaacatgat gtgctcaatt agcaacaact gatgtttctt atcccagtta
      961 tgtaatagtg ccttagttag tacaaatatt tatttgtagt cattttcaaa aaaatatata
     1021 ataagcatat aaatacatat ttactttttt aggggttatt gatgaatcaa agacacttgc
     1081 attttataac tgttcattaa caaatatatc tatgcacttt gatcttttca aaattaaagt
     1141 aaatattaac atataaaagg ggtacttcat tatgaaaaac ttagttttgt taggcggcgg
     1201 atatggtaat atgcgtatca tgtcacgcat tttaactact tctttaccac aagattatac
     1261 agtcacatta gttgatcgta tgccatttca tggattgaaa ccagaatttt atgctttagc
     1321 tgcgggcacg aaatcagata aagatgttcg tatgaaattc cctaatcatc cacaagtgaa
     1381 tacagtttat ggtgaaatta acgacataga tttagatgct caaattgtct cagtcggtaa
     1441 ttctaaaatt gattatgatg agctaatcat tggtttagga tgtgaagata aatatcataa
     1501 cgttccagga gccgaagaat atacacatag tattcaaaca ctctcaaagg ctcgggatac
     1561 tttccatagt attagtgaac taccagaagg tgctaaagtc ggtatcgttg gtgctggatt
     1621 aagcggcata gaacttgcca gcgaattaag agaaagtaga tcagacttgg aaatatatct
     1681 ttatgaccgt gggccgcgaa ttttaagaaa ttttccagaa aaattaagta agtatgttgc
     1741 gaaatggttc gccaaaaata atgttaccgt tgttccaaat tcaaatatta ataaagttga
     1801 acctggtaaa atatataact gtgatgaacc taaagatatt gatttagttg tatggacagc
     1861 aggaattcaa cctgttgaag ttgttcgtaa cttgccgatt gatataaata gtaatggacg
     1921 cgtgatagtt aaccagtatc atcaagtacc aacatatcgt aacgtctatg tagttggtga
     1981 ttgtgctgat ttaccacatg cgccaagtgc tcagttagcc gaagttcaag gtgatcaaat
     2041 tgccgatgtg cttaaaaagc aatggctaaa tgaaccatta cctgacaaaa tgccggaact
     2101 aaaggtacaa ggtatcgttg ggtcattagg agataagcaa ggttttgcat atatcatgga
     2161 ccgcactgta acaggacgac tcgcttctat tttaaaatcc ggcgtacttt ggttatataa
     2221 atatcacaat ggttaaaatt aaaaagcaat gtcagttcac actattactg acattgcttt
     2281 tagttatgtg tatattattc attcacaagc ttttggtcta taaatcgagt gatttgcttt
     2341 gtttgtatat aaccatctgc tacatattca tcgttcattg taattaatgg ataaaatagt
     2401 tcatcttgtt ctattctttc aataaattgt aaatcatgat ctgttaagtt gtcattatct
     2461 tttgtaatat ctatatacgt atatttaaat gatatatttg ggtattttct ttttaatagc
     2521 ggctgtagcc agtcataaat atctttcgat gttggcgcat taacgcaact tgcacatata
     2581 acatctgccc cataaactac cacactcacg tgctccatat atttatcccc cattggtttg
     2641 atagattttt attacactat ctattataat atactaataa agattatcaa attcatctct
     2701 cgaaaggaga cttgcctgat gcctactgaa gatacaacga tgtttgatca agtagcagaa
     2761 gttattgaac gtcttcgtcc atttttatta cgtgatggtg gcgactgctc attgattgac
     2821 gtggaagacg gtattgttaa attacaatta catggtgcat gtggtacatg cccaagttct
     2881 acaatcactc ttaaagctgg tattgagcgt gcattacacg aagaagtgcc tggtgtaata
     2941 gaagtagaac aagtattcta atataaagat attaagttaa cagaacatat tatgaataac
     3001 aacaaagaca ggaatactga attatttata atcagttatt acctgtcttt tttcgctgtc
     3061 aaattggact gatgggaaag ttattttata ctatttatct gattaattat tttattattg
     3121 ccacatatcc aagcacatat tactattcta ttctatgaaa tttaaaatat aaaaatagca
     3181 catacgctca atcaaaatcg ttgagttatg tgctatttgt attttaattt ttaggtttat
     3241 ctacttcagg ttgtggttca cctttcatat gtttcgcaat ttgctcatcc atataaaccc
     3301 aacctttcca accgatgtgt acggcatcac tgataacata tttttcatat tctttatcag
     3361 tcatatcgta aattttacca ccattatcta caacagtaga atggattttt ttataaactg
     3421 cttgacgacg ttctttatcg ataccaatgt ggtcatacca tacaccgttt gatggaatac
     3481 ttacatattg aacatctgca ccagcagcac gcattgtttt tacaagtaat tctaaatctt
     3541 ggaattctgg agaattaaca ttgaattcgt agtcacgtct aactttacgc ttactttctt
     3601 gaattaattt ccagtattga tctctaatac caaatttatt cgatgtagta tcagctttac
     3661 caatttcaac tgctttttgt ttcatctcat cccaagaagc gtctggtttt gtagcaggtt
     3721 taacatgttc taatggagat ttatccattg caaacaatga tttaatcgct tctattttaa
     3781 tcaattgatt ctctttaaaa ccagaaatat aactatcttt agtttcttta gggtttttag
     3841 cataagattt caagtattct ttattgtgta catgtggaaa ctgtaataaa cgttgtgcat
     3901 aacgacgttt taattcagta gacatgtttt tctgctggaa catttgatta atttgagttt
     3961 gagacatacg agcatcaaag ttttgattcg ttaaaccatg gtttgtaaac cattgtggtg
     4021 aaataataaa tgttaatttc ttacctttta atttatcata ctgtgatgca agttcaactg
     4081 cgttaattaa gtctgtagaa ccaccagcac ctaataagaa tgcttttttg ttggcgttat
     4141 gcttatttaa tgcaattgca ggattaaatg ggtcatcttt acctaattca ctagagccgt
     4201 atataggata atacttgttt gattcgtata acttatcttg aatgagtgtg ccttttagta
     4261 cttgatctgt caatgaagtt ctattatctt ctacagtctt ttcatttact aatcctgtaa
     4321 accaactagc aggtaataat agaaagacaa tgaataccgc tccactaatt aaaatgggta
     4381 aaaaaggttt taatttcatc gtaactcttc taatgcttca acgattttat ttggtgttgc
     4441 ccactcatct ctatcaaagt ccataataga tacttcgata tcaagtttat tttgaatctc
     4501 taataataat ccaactgttt ggaaagaatc aataatacct tcttcaaaaa tttctacgtc
     4561 tggattttct tttacaatat cattttctgc tacttctgct aataaattta atacttgttc
     4621 tctaaattcc ataattaaat tctcctttat tatataagtt tacctgagaa gattaaaaag
     4681 ccaaatgtta caaagtggaa tgtaatcaca atgctaagtg ctgttgtgaa accattttgc
     4741 caacgtggcg gatgtttctt acgccaacgt tcataatagc cataacctat aaacaatgct
     4801 gcatggtata aaccataaac aatgtaatac acttcgatac catgccaaat tcccattatg
     4861 aagaagttga ttaagaatgc cacgttagac attgcaaatt gactcttcaa taatttttta
     4921 cgagacatgt agaataaaga tctcatgtaa atacaatctc tgaaccagaa tgataatgtc
     4981 atatgccatc tattccagaa atctttaata tttttcgctt tgaaaggttt atcgaagttt
     5041 ggtggtgttt taataccgaa taaataacta aatgctatcg caaataaact ataacctgca
     5101 aagtcaaaga ataaatataa gctgtatgcg tacatatata accacaaatt gacaaagcca
     5161 tgtaagtcta attgtaacgg catgattgca tatgtgttaa taaagtaagc aacaatatat
     5221 ttatacaaga aaccaagcat aatcatgtga attgctttta atactaattc acgatattca
     5281 ttgcctgttg gtactttttt atcgtcttta acgaaacgtt tgtaacgatc gattggtcca
     5341 gatgaaatcg ttgggaagaa tgaaataaat tgaattaatt tccatacttt aatttctttg
     5401 atagaaccat cacgaatttc cataattaac tgcacacttt tgaatgtaac ataagaaatt
     5461 cctaagaaac caacaaattc aattaattta ctttcatgga agtgaatctg atgtccacct
     5521 aaccatgtac tttgtaacac tttcacaagt gctaatggca atattgataa aaccattaca
     5581 gttacaaatt ttgaaaatga attatttttt ggttttgaat gataataaaa cattatcaat
     5641 aaaacttgcc atacgacgta aataataaaa ctaattaatt gaacacttaa atacttttgg
     5701 tcaaacagat tatgtttatc agaagaaaag attaacacaa tcataaatgc tgtaactacg
     5761 ccattataaa tgtaacttcg cttacctaaa aatccaagta taatgactgg taataatgca
     5821 attaaagcaa ttaagaagaa tgtaaaatca ccatatggaa tcatccgtta attacctctg
     5881 caattttctt tctgtcaatt ttaccatttg aagtcaatgg caattgttcc atccattcaa
     5941 actttctagg aatcatatac tctggtaagc gtgatttcaa gtcattttta atatttttag
     6001 tcatttctgc attgtccgta acttcagtcg ttggcacaat tgcaccaatt aaatgaataa
     6061 ctttatcatt tttatataca ggtacaacaa tcgcttcttt tacgaactca gactggcgta
     6121 attgtgtttc aatttcttct aattccattc tgtagccatt caatttgatt tggaaatcaa
     6181 tacgaccttg aatgaaccat tgaccatttt caaacttcgc tttatcacca gtgtgatatg
     6241 tacgaatacc gtcatcgaaa ttaaatactt cagctgtttt ttggtcattt tttaagtatc
     6301 ctaaacttac actttgacct tcgataacaa gttcaccttc atctgtagta gataatcttg
     6361 cgcctggtct ttcaacgcca acaggtaatg tcggatattg atctaagatt tcttgtgtaa
     6421 tttgaatact tgtaactgct accgtagctt cagttggacc atatgtgttg taaatcgtcg
     6481 cacttgggaa acggtttact aacgcttttg ctgctctgtg aggtagaatt tcaccacaga
     6541 agaagaattc gttaagacta ccatattgtt cttcattaag cgttggtaat aataaacaca
     6601 tttccataaa tgatggtgtt gatacccaaa tgttaatcgg tgttgctgtt agcatttcat
     6661 ttaataattt aggtttatta atcatgtttt tatctacaag atttaatgta ccgcctgatg
     6721 ctaaacatgg ataaatagcc attacagata aatcaaatga aaatggcgct tggttaagcc
     6781 attgttgttc atttcctgat ttattaagtt ctaacatcca ctcagtaaat tgaactaaac
     6841 ttgcatattc aatttgaaca cctttaggtt ccccagtaga accagatgta aagattgtgt
     6901 atactgtgtc gttatcttta atctgactat caaaaattac tgggtcttga gatgttttaa
     6961 tatcttctat tgtaaatact tcgccttcta aactttcaaa tgattcatca gtcgtattaa
     7021 atacaaactc tggttgaacc ttgttaataa tcattttaat acggtcttca ggaattgaag
     7081 tgtctacagg tacatatcca catcctgctt taatggcacc aatcatccca acaatcatat
     7141 atggtgacat gtgaccgaat aaaatcatcg gtttcttact accttgtaat cgatgtgcta
     7201 atttactaga ctcatccatt aactgttgat aagttaattc atcagttgtg tgtctaacag
     7261 caatgctttg tggatttgca tccgcaaacg cttgcagctt gttaataata tctgtcatat
     7321 taagtctccc tcattagaac tcattataaa tgaagttatt gtgtgtgtcg ccactgccat
     7381 aaattaaata taaagtaata aatattgcca aatacaatag tgttaataaa tatggtttga
     7441 atgcttcaac atatttatta ggtggctgtt tacttttaga tttcatattg cacctcttaa
     7501 agttcttagt aaaaacgcct ttataaagac cgttcaatat aaaatacgtt ttaaaatttg
     7561 ttttttacaa ttcattatat cgatattcat aatgaaattc aattttaatt ttatagattc
     7621 aacatagtaa ttggttgtca tcactcaatt attgtaaatg atacactttg ttgagaacat
     7681 cattcatttt aaaggttatt taaacaataa acaattacag tctatataac aattttgtta
     7741 tatacgtcaa aatcaaataa actcatcaca ttaatatgac gagtttataa tgttattgaa
     7801 ttatcatcag cgcaaatata tacattcgca agtcaagcat aacatattta acaattgctt
     7861 tgcttgtttt accaatgatt aaaaaccata cttattttca atttactgga gtatgtggta
     7921 cctgatttgt cataaccgct tctatattat taatacataa ttgaatcata ttgtcccgtg
     7981 ttgttactga tgcgctacca atgtgtggtg taatcagaac attatcacgt cccattaatg
     8041 gatgtgtatg atcaatcggt tcatttgcta atacatctaa accacatgct aaaatttctt
     8101 tattgtctag tgcatcgatt aatgctgttt catctacaat ttgtcctcta ccgatattaa
     8161 taaaaattgc atcatttttc atttgttcaa atgcttcagc attaaattta tgatgtgttt
     8221 cttttgtaag tggcgctgta cagatgataa aatcactttc tgctaacaac gtttcaaaag
     8281 aaacatatgt tgcattaaag tccgcctctg catctttatg tcttgatcga ttatgataaa
     8341 gaatattagt attaaaccct tgcaaccttc ttgcaaaagc tttaccaata tctcccatac
     8401 catatattcc aatagttgaa ttgaagacat ctttacctga caataaataa ggaccccagc
     8461 tttgccatgc atctgcttct acatattttt cagcttctac aatacggcgt gctatagcaa
     8521 gcattaatgt aaatcctaat tctgcagttg tttcagtaag tacatttggt gtattcgtga
     8581 caaccacgtt attcgctgtt gcactttcaa catcgatgtt gtcatatcct acagccatat
     8641 tcgcaattac ttttaaattt ggtgattgcg ctaaaatttc tgcatcgatc tgttcactta
     8701 atgtaataaa acaagcatct gcgtcacgaa gtgctgtcac aaattgatct ttaggcatag
     8761 gcactaatga tttttgccac ataacaacgt caccaagttt acttaattgt tgataaaatt
     8821 tatctggaat tttcctcgaa acaactattt ttaccaaatc aattcaccta ctttataaaa
     8881 tgactatatc tatttttcta attcagctat tgcttcattt aaatctttaa aagaatacgt
     8941 tggtggcaca tttttatttt gcacatcttc taacgtagat acacctgttt gtacatgaat
     9001 cgtatccata cctacgttaa taccagacat aatatcggta tcgtacaaat cgcctaccat
     9061 agcaacttcg gatttatcta atcctaaaat ttctaatgct ttaaccataa taatcggttc
     9121 tggtttacca ataaattgtg gcgatacacc tgtcgataca cttacaacac ttgtaatagc
     9181 accattacca ggtaataaac cacgctcttt aggaattgat acatcaggat ttgtagaaat
     9241 aaatgttgca ccatttctta cacctaacgt tgcaatcgca agcttttcat atgtaacttg
     9301 ttcgtcaagt ccaataacta cataatcaac atgctcgtca tttttaatga caagtcccgc
     9361 ttcggttaac gcagtattta aaccactccc acctaacata tatactgatg ctcctggtga
     9421 ttgttctgaa atataatcag cagtggctaa cgctgacgtt acaacctctt ctggtttagc
     9481 atcaatgtgc atttcacgta atttttcagt tacttgctca ggtgtttttg ttgaattatt
     9541 agttacgtat aaatgaggaa tgccttttac atttaaataa tcgatgaatt gttttgctcc
     9601 atcaatctca tctgttccca tatacattgt gccatctaaa tcgattaaat acgctttata
     9661 ctgtttcatt attgattttc tcctttacca aaagctgtta ctggtacatt ttcttgatgt
     9721 aaaaatgtcg ttacttctgt aataaattgt ttgtaataag gtaatgcgtc atcaaacatt
     9781 ggcatgataa tcttaatatc taaggctgtg taatcatatg taaattgttt acgaataccg
     9841 acagttttat taatcgcctg ctgtgtttct ttagtaataa cattttctag ttctaatata
     9901 tcaatcacat ctttataatt accaggatcc cttaaaataa atgcatcgat aatcatattc
     9961 cctatatcta ccgatgattc tatcaacatt tgagcaatgc gttcaaatgc ataatgattg
    10021 tgcttgctct catgataatc atcagttaat gcttgtaaat aggctaattt ctgagttagt
    10081 ttatctttgt ctacaaaata cattccttgt cacctctcac ttatatatta tcataatttt
    10141 gcagtatact tcatttgtcg ttatactaac tttactactt ttaaaaggag tcgctacaaa
    10201 atgattgata tgtatttata tgatgacaac gaagagagtc aagttcagtt tgttggtttt
    10261 gttggcgagc atagtcgata tgatttgatg ttagtccata caaatagaca ttatggtaag
    10321 acactcgtac ttaatatgca aacaaataaa ttcggtatta tcggtactga tgatttaaaa
    10381 gaagaaggct atatcgctca tattttaggt gtaaatgctg aagaaggcga tgaaattact
    10441 gagtatttaa atgaagtcat tcattaaaga taaaaacccc taactagatt acaggtccat
    10501 gcttgaattg catgattccc attttcaatc tagctaaggg ttttattaat gttcagtatc
    10561 ttgaatggtt ggcttaatct tcacatcttg aagtttattg tcgtcatcta tcacatcaga
    10621 cggatgaact tcttcgatta actttttaac ctcttgttta gacaaatgtc ttaatacaaa
    10681 gtaaggacaa ccaaagttac aatattcaaa tatgtaatct tgaatatttg aaaaacgttt
    10741 actcatctct gcttttttat tagaatcttt gtaaaaacct tttaatcgta attgatcgta
    10801 accatagtca ccaactatgt aatcatattt atctaaaata tctgaatacc tagcaataaa
    10861 ttgttcttca ttaaaacatt cgcgataatt ttctattaat tcaaagtaat gttgatctac
    10921 ttttatcaaa ttcttcacct atctatgaca atgaaaggct cagtttatac ttttagtgtt
    10981 tcataaaaat aatgattact tctatgacac gttcgttttg tacaaactaa gcctttatgc
    11041 cttattaatg gttaaatatt aactatttaa ctgtgcctca ccttggcgtt gcttttcttt
    11101 agcagcttca tttacttgct catccgcatg ataagaacta cgtactaaag gtccagcttg
    11161 gcaatgttta aaccctttat ccattgccac ttttcttaat ttaccaaact ctaaaggcgt
    11221 gtaatatttt tgaaccttta aatgtttacg tgaaggttgt aaatattgac caatcgttaa
    11281 aatatctaca tcattcgcac gtaaatcatc catcgtttca taaatttctt ctatagtttc
    11341 acctaatcca accataatac ttgatttagt tgggatatcc ggttgtaatt cttttgaacg
    11401 acgtaaaaac tctaatgttc tgtcgtaagt cgcacgcgca cgaactctcg gtgttaagcg
    11461 acgaacagtt tcaatattat ggtttaaaat gtcaggtctt gacgccatta atgtttctaa
    11521 cgcatcatag tccccgccca tatctgatgg taaaatttca atcgttgtaa atggatttct
    11581 ttctctaact ttacgtactg tctcagcata aacatttgaa ccagcatctc ttaaatcatc
    11641 acgcgcaaca gcagtgataa caacgtgttt caaattcatt aattcaactg attcagctac
    11701 acgttcaggc tcatttaaat caagttcatt aggtaaacct gtcttaaccg cacaaaaacg
    11761 acaagctctt gtacatacgg cacctaaaat cataaatgtc gctgtacgac gtgcacccca
    11821 acattcatgt atattaggac atttagcttc ttcacataca gtattaagat ttttttccct
    11881 catcatcttc ttaagtcctg tatagttttc gttggtattt aattttattt tcaaccaatc
    11941 cggtttacgt aatatttcct cgttttttgt cgccataaca acacataccc tcctgttaag
    12001 tgtatcttta aacattatat cgaaattcat gacaaatgaa aatccaataa tcttactttt
    12061 taatattcca ataatttttc tttaaatata tctcttaaaa actctggcat taaatactct
    12121 tttggtaatt ctttcaatgt tgggaaataa cgaccgaacg tatacatatc tatcgtacct
    12181 agtacatatt ctttgtcgtc ttcgatttct tctccattca gatagtaacg ccctgtagaa
    12241 tgaatgtacc ctaaattata aagaatatat cctccaaata tattccctct gaaacccaaa
    12301 ccttgtgcat gttcatacat atattcttgt ttattacttt tagctataat ctcttttaat
    12361 ttcacaccaa aaagtctaac ccttaccata ttaatcggat gcggtaacat ttgatgaatg
    12421 tcatattctg tcacttcatc ttttacaata tctttaacga gtaagccagc attgatgatg
    12481 gcacattgtg catgtgtata ctcacaaaca ctttgagcta ataagtatgc agcttcagtt
    12541 atgtgattca tactacgctt aagcactact ggatgttgaa ttactgaatt ggacattaac
    12601 gtttttcctt cttcttcaaa tgaagtttca acttcaggta atgtttctaa aggaataatc
    12661 tttgcagttt tatgtactac tttatgtgcc tcaaaagtta aattaacctc tccaagataa
    12721 ttaccatact ttccagctgc cgccatcagt acaccattat tgatttcacc atgttcaaaa
    12781 taatgatgcg tatgactacc aaaaatgaca tcaatttcag gcaattcttg gcataatgtt
    12841 tcatcgaaga aaatgccaca atgacttagc acgattaata catcaaattt acctcgttga
    12901 agttcaattt cttcttttat agattcaagt ggatcggtaa caatccaatt tagtgcacga
    12961 taaaatgggg taaaaggtgc tgtcgctgca acgaatagta ttttcacacc gtctatgtcc
    13021 ttaatataag aagaaacgat attatttggt aaatgacctg attcatctat aacattgcta
    13081 catgtcacta taaattttgc ttcgtcgtaa aggtgattta aagcttcgtg tgaaatggtc
    13141 atcccttcat tattaccgat tgttgcaaca tcacattttg cttcatttag taatgccaca
    13201 ttctttttac ctaaagttgc ttcagttata ggtgcggata aatctacatg atcacctaga
    13261 tcaacatata aagaaggatg attaagtcgt ggccgatgtt ctgccatata tgctttaatg
    13321 cgttcgtatt catgtaaatg actatgaata tcgttcgtat gataaattgt aagcctcaaa
    13381 ttgacgtcct ccttttaaag ttgtattaaa tcaacgcacg aataattaaa tatataccca
    13441 ttaacaacat tactgttctc aataatgtta ctaccgtatc tgacttaatt gattgattca
    13501 ctttgacacc gattttcgca ccaaaataac tagaaataat taaaatgatt gcataacccc
    13561 aagctacgtg accttgagca atgtgcccta tagaactcat gacacttgaa aagaaaatca
    13621 tcatcatact tgttccaaca gctacatgag gtggaaatct aaatacaata agcattagtg
    13681 gcgtcattag tgcgccacct ccaataccaa ataaacctgt caatatacca attaaaaacg
    13741 ttgtaataaa agcaaacaat ggtggaacac tataatgata tgttttacct ttagcgtcta
    13801 cataagtctt ttcatacttg ggtttatcga aaattttaaa cggtttaatc ttatttctta
    13861 ccattaataa aatggctacg aaaattaaaa agataccaaa atataaatta aatgactcaa
    13921 atgttaaata tctactaatg aaggacccaa gcaatgaacc tggtaatagt ccaaataaaa
    13981 agatggaacc atttttaata tcaacttgtt ttgtttttaa atatccaagt gatgaagaaa
    14041 gtcctgtcac aattagaatg actgaagacg tccctatcgc tacttgtgtt gtaatattat
    14101 gtagtaatcc atgttcaaca ccgaggtaaa ccattgttgg aacgataata ataccgcctc
    14161 caatgcctac gatagaccct ataatcgctg acaaacctcc gattaaaact aataatgtaa
    14221 ttgttaataa catagtcgtc gcctcttaaa ataattttaa ttgttgtggt gctaaccctt
    14281 catattctat attcaataat cgctgatatg ttttggcatt atttgctgca tgaccaccag
    14341 aattattgtt aaatatgacg taaactttct tagctttttg tgctaatatt tgtgcctttt
    14401 gtgccaagtc tattaattct tgctcattat aatcatataa atagcgtaca tcgcgccatt
    14461 cttgatctga catatctttc ttagtccaac cgtaatgatt acgtccatga taacgtacaa
    14521 acgcaatttc atttgtgatt cgattgacta aaggtacact gccatctttc acttgtggtt
    14581 catcaactac tgcatgaatg atttgatgtt ctgttaaaaa tgccaatgtt tgttctttaa
    14641 atgcatcact aaaccatgat tgatgcctaa attctacaca cattggaaat gcttgtaatt
    14701 gctgtcttac atacaaaata tatttgatat tttgtgcatt gcagtcaaac catggcggaa
    14761 attgaaccaa taccattgct aattttttct gtgtatgtaa gggctctaac atattcttaa
    14821 attgatcaaa tagttcttgc cttgtatctg caaatgtttt gtagtctgca tgcaatgtga
    14881 gtgcttgatg aattttgacc acaaattcaa atgtatcagg cgtttctttt atccatttca
    14941 atatatttct ttccggttgt atcgcatagt atgtcgcatc taattcgaca accggaaaat
    15001 gtccagcata tgttttaagt ttatcggttt ggcgttctaa atcttcatat aatgaatagt
    15061 gatcacccca acctgttaat ccgatgttta tcatatatat caccaatgtc atcataccat
    15121 ataactttta tcataatcat ttcagcgaac tttagtttga tgtttttgct tgattaaaat
    15181 ctttcggcga tcttcagctt gatgttttcg tttgattaaa tttgtaaaat agaaacgcaa
    15241 tccacaaaaa tggcaagcac taaaataatg tttgggggtg cttgtgcttt tgtggattgc
    15301 ggtcgattat ttatattgca tgatttgatt aatttgattg attatattgg acatgatggt
    15361 gttggcggga tgcgttgttg ctagtcgcgg gctttgtcca ctccacatat gtattaactc
    15421 tttgtcgccg atgtttgctg cggcttttct tatgctactt gttagctcat tttgtattgg
    15481 ataatctggg atatcgcctt cgtattggga catttcttcg ataaacctat tgttgatacc
    15541 gcgtgcaagc tttccactaa acgcttttgt aatgactgta tctgtttctt tactatttat
    15601 aattgcatct cgcagtagtt ctgatgcatt actgtcttgt gatgttaaaa atgcggtgcc
    15661 catttgtacc ccttctgcac ctaagacaat acttgccaaa actcctctac catccataat
    15721 tccaccagcg gcaatgaccg gaattgaaac gacatctaca atttgtggca ctaaagatat
    15781 tgttccaacc ataggtaatt gatttttagg ttttaaaaat gaaccacgat gtccacctgc
    15841 ttcactacct tgagcaacga tagcatccat acccgctttt tcattcgcaa tagcttcatc
    15901 aacacttgtt gctgtaccta taagtttgac attcgctgct ttcaacctgc ttataatctg
    15961 ttcgcttgga attccaaaag taaaacaaca tacaggcact tgctttttaa ttatcgtatc
    16021 aatatgacac ttaaattgtt gttcttcggt aatttttaca accggctctt ctaaatgtaa
    16081 tgcgcgtcga taaggtttta accatgcatt catattttca atttgactac tggtatatga
    16141 ttgttgactt ggtacaaaga catttacgcc aaaagaattt gacgttaatt ggcgtacata
    16201 atctatttca tcttccaatt gctgcgtatt aaagtaacct gcgcctattg tgcctaaccc
    16261 accactgtta cttactgatg caactaattt cggtgtcgta cttcctgcca tacctgcttg
    16321 tataattgga tattcaatac ttaacatttg agtaagtcga ttcttattcc acatagctgt
    16381 tcgctcctta tatagatacg ttgcgatttt tccgttgttg aaattgaatt tgctgttgag
    16441 aaagtttttc tttttccttt ttatccatct catcttcaat ttccatacct aataattctt
    16501 caattaagtc ttcatgtgac actatcgctt cagtaccacc aaattcgtcc aacacaattg
    16561 ctaaatgttt tctagaaata gtcatcttac gtaataccca ttcagcttta ttgtgttcat
    16621 tcacaaataa tggcttagct gaatagtttg taatttgatt ttctttttta ttactccaag
    16681 ccaacagata tttagaatga aacaccccaa taatgttatc aatatctccc tcgtacactg
    16741 gatatctagt gtatggctta ttcataaccg tttcataaac ttcttcgtat gtcgcatttg
    16801 aagcaaatgc cgtcacatta attctaggtg ttgtatctac atcttttact tttaaatttt
    16861 caaaattaat gacaccttcc aacctactcg tctcaatttc atttaaagca ccttcatgtc
    16921 cagcaattgc taacattgtt ttaaattctt cttttgaaaa ttgatgttct tgaggttggc
    16981 ccttagataa acttcgatta atactgtccg tcaacttatt taaaagtaat gtgataggac
    17041 ggaacacaat gacacaaata ttaataattg gatatacaag ccttgttatt ttatctggaa
    17101 atgttgcagc gacagacttg ggaatcactt cggagatcaa aatgataaca actgttaaaa
    17161 cagctgatgc aataccaacg ctaatccccc aacgtaaagc cataattgta acaagtgttg
    17221 gtaataaaat attcgcgaca ttattcccaa ttagaatcgt tgtaataaac tcacttggtt
    17281 tttcaagtaa ctttacaatg ccttttgctt ttttatcacc tttgtcagct tcagttttaa
    17341 attttgtttt attggcagcc gttaatgccg tctcgcttcc tgaaaagaaa aacgaaataa
    17401 atatcaatat aattatggca atgatcacgc gtgtagtctc cttattgtca tatcttattt
    17461 ttattgtagt tacttaattc ccgacgttct cgtttaataa accagttaaa attcattata
    17521 tataatagtc ttaataatga taattaatat gcatttttag tcatagttac tttaatgcat
    17581 tcattagtaa aatgatgcta tagttcgagc tgtttgttat taataaattt gcataatgaa
    17641 aaatttcaac aaattcagtt tatgacatag ttataatcat ttgaaaccat tataa
//
LOCUS       DALAGF010000052        17422 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.052, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000052 DALAGF010000000
VERSION     DALAGF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17422
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.052"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            166..2262
                     /gene="pnp"
                     /locus_tag="O2692_001593"
     CDS             166..2262
                     /gene="pnp"
                     /locus_tag="O2692_001593"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCZ4299226.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            2498..4171
                     /locus_tag="O2692_001594"
     CDS             2498..4171
                     /locus_tag="O2692_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCZ4299227.1"
                     /translation="MSLIKKKNKDIRIISLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            4427..6796
                     /locus_tag="O2692_001595"
     CDS             4427..6796
                     /locus_tag="O2692_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCZ4299228.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHREVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLTEQTHNSIESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            6801..7514
                     /locus_tag="O2692_001596"
     CDS             6801..7514
                     /locus_tag="O2692_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCZ4299229.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNESDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            7545..8810
                     /locus_tag="O2692_001597"
     CDS             7545..8810
                     /locus_tag="O2692_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCZ4299230.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            8810..10096
                     /locus_tag="O2692_001598"
     CDS             8810..10096
                     /locus_tag="O2692_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCZ4299231.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFDTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            10096..10800
                     /locus_tag="O2692_001599"
     CDS             10096..10800
                     /locus_tag="O2692_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCZ4299232.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            10905..11732
                     /locus_tag="O2692_001600"
     CDS             10905..11732
                     /locus_tag="O2692_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HCZ4299233.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            11751..12143
                     /locus_tag="O2692_001601"
     CDS             11751..12143
                     /locus_tag="O2692_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ4299234.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            12177..12755
                     /gene="pgsA"
                     /locus_tag="O2692_001602"
     CDS             12177..12755
                     /gene="pgsA"
                     /locus_tag="O2692_001602"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCZ4299235.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
     gene            12982..14133
                     /locus_tag="O2692_001603"
     CDS             12982..14133
                     /locus_tag="O2692_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HCZ4299236.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLTNHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMSKSRNLLKNRSQNYALIRLLNWLRTTN"
     gene            14298..15341
                     /gene="recA"
                     /locus_tag="O2692_001604"
     CDS             14298..15341
                     /gene="recA"
                     /locus_tag="O2692_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCZ4299237.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            15695..17254
                     /gene="rny"
                     /locus_tag="O2692_001605"
     CDS             15695..17254
                     /gene="rny"
                     /locus_tag="O2692_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCZ4299238.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
BASE COUNT         6385 a         2341 c         3374 g         5322 t
ORIGIN      
        1 ataacttata tttatactga taagggtggg acgataagtc tattttgtaa ataatagatg
       61 gatatcccgc tctctttttt tccaattcaa tattttataa ctaatattaa aatacgataa
      121 taaatgatat gatataacta ttagattcaa gagaggagat ttataatgtc tcaagaaaag
      181 aaagttttta aaactgaatg ggcaggaaga tctttaacga ttgaaacagg gcaattagct
      241 aaacaagcaa atggcgctgt attggttcgt tatggagata cagtcgtgtt atcgacggca
      301 actgcatcaa aagaacctcg tgatggagat ttcttcccat taacagtgaa ctatgaagaa
      361 aaaatgtacg ctgcgggtaa aattcctggt ggatttaaaa agagagaagg acgtcctggt
      421 gacgatgcaa cattaactgc gcgattaatt gatagaccaa ttagaccttt attccctaaa
      481 ggatataagc atgatgttca aattatgaac atggtattaa gtgcagatcc tgattgttca
      541 ccacaaatgg ctgcaatgat tggttcatct atggcgctta gtgtgtcgga tattccattc
      601 caagggccaa tcgccggtgt aaatgtgggt tatattgacg gtaaatatat cattaaccca
      661 acagtagaag aaaaagaagt ttctcgttta gaccttgaag tagctggtca taaagatgcg
      721 gtaaacatgg tagaggcagg cgctagtgag attactgaac aagaaatgtt agaggcgatt
      781 ttctttggtc atgaagagat tcaacgttta gttgatttcc aacaacaaat cgtcgaccac
      841 attcaacctg ttaaacaaga atttattcca gcagagcgtg atgaagcgct agttgaacgt
      901 gtaaaatctt taaccgaaga aaaaggactt aaagaaacag ttttaacatt tgataaacaa
      961 caacgagatg aaaatcttga taacttaaaa gaagaaatcg tcaatgaatt tatcgatgaa
     1021 gaagatccag agaatgaatt acttattaaa gaagtttatg caattttaaa tgaattagtg
     1081 aaagaagaag ttcgacgttt aattgcagat gaaaaaatta gaccagacgg ccgtaaacct
     1141 gatgaaatcc gtccattaga ttctgaagtt ggtattttac ctagaacgca tggttcaggt
     1201 ctatttacac gtggtcagac tcaagcactt tcagttttaa cattaggtgc tttaggcgat
     1261 tatcaattaa ttgatggttt aggacctgaa gaagaaaaaa gattcatgca tcattacaac
     1321 ttcccgaatt tttcagtagg tgaaactggt ccagtacgtg cgccaggtcg tcgtgaaatt
     1381 ggacatggtg cgttaggtga aagagcatta aaatatatta ttcctgatac tgctgatttc
     1441 ccatatacaa ttcgtattgt aagtgaggta cttgaatcaa atggttcatc atctcaagcg
     1501 tcaatttgtg gatcaacatt agcattaatg gatgcgggcg taccgattaa agcaccagtt
     1561 gctggtattg ctatgggcct tgttacacgt gaagatagct atacgatttt aactgatatc
     1621 caaggtatgg aagatgcatt aggtgatatg gactttaaag tcgctggtac taaagaaggt
     1681 attacagcaa tccaaatgga tattaaaatt gacggtttaa cgcgtgaaat tatcgaagag
     1741 gctctagaac aagcgagacg tggtcgttta gaaataatga atcatatgtt acaaacaatt
     1801 gatcaaccac gtactgaatt aagtgcttac gcgccaaaag ttgtaactat gacaattaaa
     1861 ccagataaga ttagagatgt tatcggacct ggtggtaaaa aaattaacga aattattgat
     1921 gaaacaggtg ttaaattaga tattgaacaa gatggtacta tctttattgg tgctgttgat
     1981 caagctatga taaatcgtgc tcgtgaaatc attgaggaaa ttacacgtga agcggaagta
     2041 ggtcaaactt atcaagccac tgttaaacgt attgaaaaat acggtgcgtt tgtaggccta
     2101 ttcccaggta aagatgcgtt gcttcacatt tcacaaattt caaaaaatag aattgaaaaa
     2161 gtggaagatg tattaaaaat cggtgacaca attgaagtta agattactga aattgataaa
     2221 caaggtcgag taaatgcttc acatagagca ttagaagaat aatatttaaa gtcatatgac
     2281 gacaatgtat cgtcatgtga tttttttatg ccacttttta cgaagtgacc cgttttgaat
     2341 ttgttgtatt gaacatttta aaacgcttta ttattttgtg tgcaactgtt aattatcctg
     2401 tatgtatagt gattaatagt gtacatcaag tgttttttaa cttataatga atagtgagtt
     2461 tatatatgga cgggtaacaa atttaggagg taagattttg agtttaataa agaaaaagaa
     2521 taaagatatt cgcattatat cattaggcgg tgttggcgaa attgctaaaa atatgtatat
     2581 cgttgaagta gacgatgaaa tgtttatgtt agatgctgga cttatgtttc cagaagacga
     2641 aatgctaggt attgatattg ttataccaga catttcatac gtacttgaaa ataaagataa
     2701 attgaagggt atattcctta cacacggaca tgagcacgcg attggtgcag tgagttatgt
     2761 tttagaacaa ttagatgcac cagtatatgg atctaaattg acaatagcgt taattaaaga
     2821 aaatatgaaa gcccgtaata ttgataaaaa agttcgctac tatacagtta ataatgattc
     2881 aattatgaga ttcaaaaacg tgaatattag tttctttaat acgacacaca gtattcctga
     2941 tagtttaggt gtttgtattc acacttcata tggtgccatt gtgtatacag gtgaatttaa
     3001 gtttgaccaa agtttacatg gacattatgc accagatatt aaacgtatgg cagagattgg
     3061 tgaagaaggc gtatttgtct taatcagtga ttctactgag gcagagaaac ctggatataa
     3121 tactccggaa aatgtgattg aacatcatat gtatgatgct tttgcaaaag tgcgaggtcg
     3181 cttgatagtt tcatgttatg cttcgaactt tatacgtatt cagcaagttt taaatattgc
     3241 tagcaagcta aatcgtaaag tgtcattttt aggaagatca cttgaaagtt catttaatat
     3301 tgctcgtaaa atggggtatt tcgacattcc taaagatttg ctaattccta taacagaagt
     3361 tgataattat cctaaaaatg aagtgataat tatagctact ggtatgcaag gagaacctgt
     3421 agaagcctta agtcaaatgg cgcaacataa gcataaaatt atgaatatcg aagaaggcga
     3481 ttctgtattt ttagcaatta cggcttctgc taatatggaa gttatcattg cgaatacatt
     3541 aaatgagctt gtacgtgctg gcgcacatat tattccaaat aacaaaaaga ttcatgcttc
     3601 aagtcatggt tgcatggaag aattaaaaat gatgattaat attatgaaac ctgaatactt
     3661 tattcctgta caaggtgaat ttaaaatgca gatagcacat gcgaagctag cagctgaagc
     3721 aggtgttgca ccagaaaaga ttttccttgt ggaaaaagga gatgtcatta attacaacgg
     3781 taaagatatg atattaaatg aaaaggtaaa ttcaggaaat attttaatag atggcattgg
     3841 tattggggat gtaggaaata tcgtgttgag agaccgtcat cttttagcag aagatggtat
     3901 ctttattgct gttgtaacgt tagatcctaa aaatagacgt atagctgcgg gacctgaaat
     3961 tcaatctcgt gggtttgtat atgtacgtga aagtgaagac ttattacgtg aagcagaaga
     4021 gaaagtacgt gaaatagtag aggctggttt acaagaaaaa cgcatagaat ggtctgaaat
     4081 taaacaaaat atgcgtgatc aaattagtaa actattattc gaaagtacaa aacgtcgtcc
     4141 tatgattatt ccagtaattt ctgaaattta atcaaaaagt cattaacata aaagaggtca
     4201 gaacaagtca ctgaaatata atggttgtca tggacaattt acttatattt tatgatggtc
     4261 aattgaaggg gtaacgatta atctgttatc ttaagtaaat tgatacatag atgatattgt
     4321 tctaacctct ttcatcgtct gtttggacta catattctaa acatcaaata ggaaattata
     4381 tataataacg tcgttttaac taaggcaaca taaggaggtg cgtcaattgg cacaagcaaa
     4441 aaagaaatcg acagctaaga aaaaaacaac atcaaaaaaa agaacaaatt cgaggaaaaa
     4501 gaagaatgat aatccgatac gttatgtcat agctatttta gtagttgtat taatggtgtt
     4561 gggtgttttc caattaggaa taataggtcg tctaattgac agcttcttta attatttatt
     4621 tgggtacagt agatatttaa catatatttt agtactctta gcaactggtt ttattacata
     4681 ctctaaacgt attcctaaaa ctagacgaac ggctggttcg attgtattgc aaattgcatt
     4741 gctatttgta tcacagttag tttttcattt taatagtggt atcaaagctg aaagagaacc
     4801 tgtactttct tatgtgtatc agtcatacca acacagtcat ttcccaaatt ttggtggcgg
     4861 tgtattaggc ttttatttat tagagttaag cgtaccttta atttcattat ttggtgtatg
     4921 tattattact attttattat tatgctcaag tgttatttta ttaacaaacc atcaacatcg
     4981 tgaagttgca aaagttgcac tggaaaatat aaaagcttgg tttggttcat ttaatgaaaa
     5041 aatgtcggaa agaaaccaag aaaaacaatt gaagcgtgaa gaaaaagcaa gacttaaaga
     5101 agaacaaaag gcacgtcaaa atgaacagcc acaaataaaa gatgtgagtg attttacgga
     5161 agtgcctcaa gaaagagata ttccaattta tgggcatact gaaaatgaaa gtaaaagcca
     5221 gagtcaacca agtcgaaaaa aacgagtgtt tgatgcagag aatagttcga ataacatcgt
     5281 aaatcatcat caagcagatc agcaagaaca attaacagaa caaactcata acagtattga
     5341 aagtgaaaac actattgaag aagctggtga agttacgaat gtatcgtatg ttgttccacc
     5401 gttaacttta cttaatcaac ctgcaaaaca aaaagcaaca tctaaagctg aagtgcaacg
     5461 taaaggacaa gtactagaga atacattaaa agattttggg gtaaatgcaa aagtgacaca
     5521 aattaaaatt ggtcctgcag taactcaata tgaaattcaa ccagctcaag gggttaaagt
     5581 gagtaaaatt gtaaacttgc ataatgatat tgcattagct ttagcagcaa aagatgttag
     5641 aatcgaagcg ccaatacctg gtcgttctgc agtaggtatt gaagtgccaa atgagaaaat
     5701 ttcattagtt tcactaaaag aagttttaga tgaaaaattc ccgtctaata ataaactaga
     5761 agttggatta ggaagagata tatcaggtga tccaattact gttccactaa atgaaatgcc
     5821 acacttattg gtggcaggat cgacgggtag tggtaaatct gtttgtataa atggtattat
     5881 tacaagtatt ttattaaatg ctaagccgca tgaagttaaa cttatgttaa tcgatccgaa
     5941 aatggttgaa ctaaatgttt ataacggaat tccacactta ttaattccgg ttgttacaaa
     6001 tcctcataaa gctgctcaag ctttagaaaa aattgtagct gagatggaaa gacgttatga
     6061 tttattccaa cattcatcaa ctagaaacat taaaggttat aacgaattaa tccgtaagca
     6121 aaatcaagaa ttagatgaga agcaaccaga attaccttat atcgttgtta ttgtagatga
     6181 gcttgcagat ttaatgatgg tagctggtaa agaagttgaa aatgcgattc aacgtattac
     6241 acaaatggca cgtgcagcag gtatacattt aattgtagcg acacaaagac cttctgtgga
     6301 tgtaattaca ggtatcatta aaaataatat tccatctaga atagcttttg ctgtgagttc
     6361 tcaaacagat tcaagaacta ttattggtac tggcggcgca gaaaagttac ttggtaaagg
     6421 tgacatgtta tacgttggaa atggtgactc atcacaaaca cgtattcaag gggcgttttt
     6481 aagtgaccaa gaggtgcaag atgttgtaaa ttatgtagta gaacaacaac aggcaaatta
     6541 tgtaaaagaa atggaaccag atgcaccagt ggataaatcg gaaatgaaaa gtgaagatgc
     6601 tttatatgat gaagcgtatt tgtttgttgt tgaacaacaa aaggcaagta catcattgtt
     6661 acaacgccaa tttagaattg gttataatag agcatctagg ttgatggatg atttagaacg
     6721 caatcaggta atcggtccac aaaaaggaag caagcctaga caagttttaa tagatcttaa
     6781 taatgacgag gtgtaaaaaa atgtcagaaa tgaatgcggt atataacgtt aaacaataca
     6841 ttttaaattt gattaagcaa aataaattgg aatatggtga ccaacttcca agtaatttat
     6901 caattgccag agaattaaat gtaaaaaccg acgatgttta tgaagcaatt caggcattga
     6961 ttactgaaca agtcattaaa gataattttg aagagggcac aagtgttaag tcactgcccc
     7021 ctttctttta tccattgaat gaacttataa gtattgggca aatgattaaa aatgcaggat
     7081 ttgaatgcgg aactgaatac ttaaattttg atgagcaacc agcaactatg ttagatgcaa
     7141 atttgttgag cgttgaagaa ggatatccag taaccattat agaacgatta cgaactgccg
     7201 atggagaacc ggtcgtctat tgtttagata aaattgctaa aaaagaatta acatgtacag
     7261 agtatcaaat gagcaatgga tcgattctaa gtgcgataaa agaacaaagt aatcataata
     7321 tttgttatgc tgatacagaa attgaagcgg taaattatga acctcgaata tccgaagtac
     7381 tgaatgcttc gccacacgaa ggtttgattt tattaaaaat tacgcactat aatgaatcag
     7441 atgaacctat tttgtattca ttaaattata tgaaaaatag cttagttcaa tttaaaatca
     7501 ctagaaaaat atagatgtaa gaagatatag aggaggctat atatttgagt agacaatctc
     7561 aaccaaatat acatatcaaa gtttcaccaa caactaaatt taaaacaact actatagttt
     7621 ttaaatttat ggcaccttta gaatatgaca caataacagc tagatcatta ttaagcaaat
     7681 tattagttcg agcaactaag aaatggccaa ccgataagtc gtttaataat cacttagccg
     7741 atttatatgg tgcgtatgtg aatagtacaa tttcaaaatt caaagatcag catgtcatta
     7801 cattttcatt agaaattgtt aatgaacgtt atttaagaaa cggtgaatca ttatttaatc
     7861 aaggattaga tttattacaa gaaatcattt ggaatccatt aattgaaaat aaagcattca
     7921 atgataattt tgttaaccaa gagaaaacat tattagccaa aaaaatagaa gcaatggtag
     7981 ataataaagc acaatattcg tttttaaaat tactcgacca tatgtttgaa aatgaagcat
     8041 ataaatactt atctacagga caactagaac aaatcccaca tattactgct gaaacactat
     8101 atcatacata tcaatcaatg attaataatg atcaatgttc tgtttatgtt gtcggcaatg
     8161 tagaacctga aagtgttgag aaacaaatac gtgaaaaatt tgcacttaaa ccattcgata
     8221 aacatcaatt ccaacattct actcatcatt tacacgatga agaagttgat tatattgttg
     8281 aatatgatga cgtggatcaa gctaaattaa atatgggata ccgttttcca acacaatatg
     8341 gacaaagtgg atatgctgcc tttgttgtat ttaacatgat gtttggagga gatccttcat
     8401 ctgttttatt taatgaagtg cgagaaaagc aaagtttagc gtactctata cattcacaaa
     8461 ttgatggcaa aaatggctat ttatttgttt tgagtggggt ttcaagtgat aagtacgaaa
     8521 ctgcaaaaga cactattata agtgaatttg aaaaaataaa agcaggagat ttcactgaag
     8581 aaaaattaga gttagctaaa aaagtaatca tttctcatcg atatgaatct gaagatcgtc
     8641 cgaaaagtat tatagagatt atgcataacc aaatattatt agagcaacca caaagcaaag
     8701 aaacatttat aaatgatata cagaaggtaa gtcgcgaaga tattgtttct gttgctgaaa
     8761 aagcattttt agatacaatc tatgtgttga caaaaggagg ggataaataa tgaaagagcg
     8821 ttattatgaa ttaatagacg aaagagtatt cgaacaagaa ttagaaaatg gtttacgatt
     8881 atttattatc cccaaaccag gttttcaaaa gacatttgtc acttacacta cacaatttgg
     8941 ttcattagat aatcaattca aaccccttgg acaagaccaa tttgttactg tacctgatgg
     9001 agttgctcac tttttagaac ataaattatt tgaaaaagaa gaagaagact tatttactgc
     9061 gtttgctgaa gataacgcac aagcaaatgc gtttacaagc tttgatcgta caagctactt
     9121 gttcagtgca actgataata ttgaaaacaa cattaaacgt ttacttacaa tggttgaaac
     9181 gccttatttt acaaaagaaa ctgttgataa agaaaaaggt attattgcag aagaaataaa
     9241 aatgtatcaa gagcaacctg gatataaatt aatgtttgat acattgcgtg caatgtatca
     9301 acaacatcca atacgtgttg atattgccgg tagtgtagaa agtatatacg atattacaaa
     9361 agatgatttg tatctatgtt atgaaacgtt ttatcatcca tcaaatatgg ttttatttgt
     9421 tgttggcgat gtggatcctg aagcaatatg tcgaatagta aaacaacacg aggatgctcg
     9481 taataaagtt aaccaaccca aaatcgaacg aggacttgtt gatgaaccgg aggatgttaa
     9541 agaagcattt gttactgaat ctatgaaaat tcaatcacca agactaatgc ttggttttaa
     9601 aaataaacca ttacaagaag cgcctcaaaa atatgtacaa cgtgatttag aaatgtcatt
     9661 attctttgag ttaatttttg gggaagaaac agatttttat cagaatttat taaacgaagg
     9721 acttatcgat gatacatttg gttatcaatt tgtactagag ccgacgtata gtttttcaat
     9781 cgtgacaagt gctactgaag aaccagataa attaaaaaaa ttattattag atgagttgcg
     9841 tgataaaaaa ggcaatttcc aagatgcaga agcatttgaa cttttgaaaa agcaatttat
     9901 aggtgaattc atatcaagtt taaactcacc tgaatatatt gctaatcaat atactaaatt
     9961 gtattttgaa ggtgttagcg tgtttgacat gttagacatt gttgaaaata tcacgttaga
    10021 tagtattaac gaaacgtcat cgttatattt aaatttagat cagcaagtcg atagtcgttt
    10081 ggagattaaa aagtaatgaa agcattagta ttaggtggtt ctggttcaat tggttctgag
    10141 atagtcaaac aattattaac tgatggattt gaagtttatg tgcaatatta tcgtactgat
    10201 ataaatgaat taactagcaa atttaatgat gataaagttc gttttataca agcggattta
    10261 tctcaaacaa ttgatattga caaaacattt ggtgacatta aatcattaga ctgtttaata
    10321 tatgcaagtg gtcagtcttt atatggtgtt ttacaagata tgaaagacca tgatattgat
    10381 gcatgttatc agttaaatgt cttgcaatta attcgattat gtagatattt cgttgatgtt
    10441 ttacgtcaaa gtgccaatgg aagaattatt gtaatttcat caatttgggg tgagacagga
    10501 gctagtatgg aaactattta ttcggcgatg aaaagtgcac aattaggttt cgttaaggcg
    10561 cttagtcaag agcttgcact aacatcagtg acagtaaatg ctatcgcacc tggatttgta
    10621 gccggtaata tggcaagtga gtggcaagaa gatgaacttc aagcaatgat aactgaatta
    10681 ccacaacagc gattggtttt accgagtgag gttgctcata catgcgccta tttatatcac
    10741 ccaaatgcta gaagtgttac tggaactata cagaaagtta atggtgcttg gtatatttaa
    10801 aatatagaac tatttttatg agcaaatgtt tttatgattg atgttataat atgaatatta
    10861 caatgaatca taatttgtta attaaacaag gggtgaggtt caccatgaca gttgcagaga
    10921 aaaaagaatg gtacctagaa tacgaaattg aaattaatag accgggtctt ttaggtgatg
    10981 tatctagttt attaggtatg ttaggtataa gtattgttac aattaatggt gttgatcaag
    11041 gtaaacgagg ccttttaatt aaaacagaca atcttgaaaa agttgaacgt tttgagcaaa
    11101 tagctcgtgg tataaatgaa attgaaataa caaagcttaa aaaaccagaa ttaagagacc
    11161 gtcttgcagt aagacatgga agatatattg agcaagatgc aaaagataag aaaacctttc
    11221 gatttgagcg tgaagattta ggcttgttag tagacttttt agctgaattg ttcaaagaag
    11281 aaggtcataa gttgattggc attagaggta tgccacgggt tggtaaaact gaatcaattg
    11341 ttgcgggaag tgtttgtgca cataagagat ggttatttat tagttctact ttaataaaac
    11401 aaactgtacg tagctctcta attaaagggg aatatgatgc caatcatgta tacattattg
    11461 atggtgcagt tactgccaga gaatctaatc caaaacatca agagcttgtt aacgaagtta
    11521 tgacgttacc atcaatcaaa gtcgttgaac atccagattt atttgttgaa acaagtactt
    11581 gtacaatgga agattttgac tatattatcg aattgagaga aaatgaaaat caagaaatac
    11641 attacgaaga aatgaagaaa cagacagtcc aaagtaagaa taatttagat tttggagatc
    11701 cgtttggtgg tggttttggt ttcttcgagt aagtgtaagg aggctatgaa ttgaaaacgg
    11761 tcggtgaagc gctaaaaggt agacgtgaaa ggttaggaat gactttaaca gaattagagc
    11821 aacgtactgg aattaaacgt gaaatgctag tgcatattga aaataatgaa ttcgatcaac
    11881 taccgaataa aaattacagc gaaggattta ttagaaaata tgcaagcgta gtaaatattg
    11941 aacctaacca attaattcaa gctcatcaag atgaaattcc atcgaaccaa gccgaatggg
    12001 acgaagtaat tacagttttc aataataata aagacttaga ttataagagt aaatcaaaag
    12061 agccaataca attattagta atcatgggta ttacagtttt aataacttta ttgttatgga
    12121 tcatgttagt tttaatattt taacagaaat aaattagtga gaaatgagga tgtataatga
    12181 atattccgaa ccagattacg gtttttagag tagtgttaat accagttttt atattgtttg
    12241 cgttagttga ttttggattt ggcaatgtgt catttctagg aggatatgaa ataagaattg
    12301 agttattaat cagtggtttt atttttatat tggcttccct tagcgatttt gttgatggtt
    12361 atttagctag aaaatggaat ttagttacaa atatggggaa atttttggat ccattagcgg
    12421 ataaattatt agttgcaagt gctttaattg tacttgtgca actaggacta acaaattctg
    12481 tagtagcaat cattattatt gccagagaat ttgccgtaac tggtttacgt ttactacaaa
    12541 ttgaacaagg attcgtaagt gcagctggtc aattaggtaa aattaaaaca gcagttacta
    12601 tggtagcaat tacttggttg ttattaggtg atccattggc aacattgatt ggtttgtcat
    12661 taggacaaat tttattatac attggcgtta tttttactat cttatctggt attgaatact
    12721 tttataaagg tagagatgtt tttaaacaaa aataaatatt tgtttatact agatttcatt
    12781 ttcatatgga atctagtttt tttaatccca attttagaaa ttagccacgc aattgtttat
    12841 aatgatatat tgtaaaacaa tatttgttca tttttttagg gaaaatctgt agtagcatct
    12901 gatacattga atctaaaatt gatgtgaatt tttaaatgaa atacatgaaa aaatgaatta
    12961 aacgatacaa gggggatata aatgtcaatt gccattattg ctgtaggctc agaactattg
    13021 ctaggtcaaa tcgctaatac caacggacaa tttctatcta aagtatttaa tgaaattgga
    13081 caaaatgtat tagaacataa agttattgga gataataaaa aacgtttaga atcaagtgta
    13141 cgtcatgcgc tagaaaaata tgatactgtt attttaacag gtggcttagg tcctacgaaa
    13201 gatgacttaa cgaagcatac agtggcccag attgttggta aagatttagt tattgatgag
    13261 ccttctttaa aatatattga aagctatttt gaggaacaag gacaagaaat gacacctaat
    13321 aataaacaac aggctttagt aattgaaggt tcaactgtat taacaaatca tcatggcatg
    13381 gctccaggaa tgatggtgaa ttttgaaaac aaacaaatta ttttattacc aggtccaccg
    13441 aaagaaatgc aaccaatggt gaaaaatgaa ttgttgtcac attttataaa ccataatcga
    13501 attatacatt ctgaactatt aagatttgcg ggaataggtg aatctaaagt agaaacaata
    13561 ttaatagatc ttatcgataa acagactaat cctacgattg cgcctttggc gggaagtcat
    13621 gaagtatata ttagattgac tgcaaatgcc gactcaaaag aacaagcaca atcattgatt
    13681 caacctgtta aacaagaaat tcttgatcgt attggagaat attattatgg ttcagatgac
    13741 acattaattg agcaagctgt aataaagaaa attcatgaac cttttgtaat atatgatggt
    13801 attactaatg gtgctttata tcatcgattg aaagaagtgg atttaaacga tgttctaaag
    13861 ggtatgatta atcacaatga aaactttgtt gatattaata aacctattga gcagcaatta
    13921 aaagatgcag tgcaatttgt taataaattg tttaatgtgt catcagcaat tattctatta
    13981 gagtatgatg gtgtagttca tataggctat gataataact ttgaatttaa aactgagcaa
    14041 tttaaaatgt ctaaatctag aaatttatta aagaacagaa gtcaaaatta tgcgctcata
    14101 agattattaa attggcttag aacaacaaat taattgtatt atcgataaaa atataagcac
    14161 gtttgttcgt ttttgcgttt tgatttcaag attttatacg aacaaatatt cgcaaaacac
    14221 ttgtatttta ttttgaatcc ttgtatagta ttggtaagat aattaaaaga tagcaatttc
    14281 aattaggagg tctcgctttg gataacgatc gtcaaaaagc tttagataca gtaattaaaa
    14341 atatggagaa atctttcggt aaaggtgccg taatgaagtt gggtgacaat ataggtcgcc
    14401 gagtttcaac tacatcaact ggttcagtta cattagataa tgcgctaggt gtaggtggct
    14461 atcctaaagg acgaattatt gaaatttatg gtcctgaaag ttctggtaag acaacagtag
    14521 cgcttcacgc tattgctgaa gtacaaagta atggcggggt ggcagcattt atcgatgctg
    14581 aacatgcttt agatccagaa tatgctcaag cattaggcgt agatatcgat aatttatatt
    14641 tatcgcaacc ggatcatggt gaacaaggtc ttgaaatcgc cgaagcattt gttagaagtg
    14701 gtgcagttga tattgtagtt gtagactcag ttgctgcttt aacacctaaa gctgaaattg
    14761 aaggagaaat gggagacact cacgttggtt tacaagctcg tttaatgtca caagcgttac
    14821 gtaaactttc aggtgctatt tctaaatcaa atacaactgc tattttcatc aaccaaattc
    14881 gtgaaaaagt tggtgttatg ttcggtaatc cagagactac accaggtgga cgtgcattaa
    14941 aattctatag ttcagtaaga ctagaagtac gtcgtgcaga acagcttaaa caaggacaag
    15001 aaattgtagg taatagaact aaaattaaag tcgttaaaaa taaagtggca ccaccattta
    15061 gagtagctga agttgatatt atgtatggac aaggtatttc taaagagggt gaacttattg
    15121 atttaggtgt tgaaaacgac atcgttgata aatcaggagc atggtattct tacaatggcg
    15181 aacgaatggg tcaaggtaag gaaaatgtta aaatgtactt gaaagaaaat ccacaaatta
    15241 aagaagaaat tgatcgtaaa ttgagagaaa aattaggtat atctgatggt gatgttgaag
    15301 aaacagaaga tgcaccaaag tcattatttg acgaagaata gtacacaaat ttatatctat
    15361 agttaaactt agcaaatatc cttataggat tgattgaaag tgatattcat ctcataaagc
    15421 tagaataata tctaacttta tgggatacac tacaaatcga gactataagg ttttttattt
    15481 tatttattat tacattatca atagttttat aatcgagctt caaaacttta gaaaatagta
    15541 gaaatagcat tcaatatagt gcaaaagtgc aaattgataa cttgacactt atctcctata
    15601 aaccgtacaa ttaatttgta tgatttatat ataatttcat aaagtcatat tgaatttcat
    15661 ataaagagca aaccctagaa aaggaggtgt ttgtgtgaat ttattaagcc tcctactcat
    15721 tttgctgggg atcattctag gagttgttgg agggtatgtt gttgcccgaa atttgttgct
    15781 tcaaaagcaa tcacaagcta gacaaactgc cgaagatatt gtaaatcaag cacataaaga
    15841 agctgacaat atcaaaaaag agaaattact tgaggcaaaa gaagaaaacc aaatcctaag
    15901 agaacaaact gaagcagaac tacgagaaag acgtagcgaa cttcaaagac aagaaacccg
    15961 acttcttcaa aaagaagaaa acttagagcg caaatctgat ctattagata aaaaagatga
    16021 gattttagag caaaaagaat caaaaattga agaaaaacaa caacaagtag atgcaaaaga
    16081 gagtagtgtt caaacgttaa taatgaagca tgaacaagaa ttagaacgca tctccggtct
    16141 cactcaagaa gaagctatta atgagcaact tcaaagagta gaggaagaac tgtcacaaga
    16201 tattgcagta cttgttaaag aaaaagaaaa agaagctaaa gaaaaagttg ataaaacagc
    16261 aaaagaatta ttagctacag cagtacaaag attagcagca gatcacacaa gtgaatcaac
    16321 ggtatcagta gttaacttac ctaatgatga gatgaaaggt cgaatcattg gacgagaagg
    16381 acgaaacatc cgcacacttg aaactttaac tggcattgat ttaattattg atgacacacc
    16441 agaagcggtt atattatctg gttttgatcc aataagaaga gaaattgcta gaacagcact
    16501 tgttaactta gtatctgatg gacgtattca tccaggtaga attgaagata tggtcgaaaa
    16561 agctagaaaa gaagtagacg atattattag agaagcaggt gaacaagcta catttgaagt
    16621 gaacgcacat aatatgcatc ctgacttagt aaaaattgta gggcgtttaa actatcgtac
    16681 gagttacggt caaaatgtac ttaaacattc aattgaagtt gcgcatcttg ctagtatgtt
    16741 agctgctgag ctaggcgaag atgagacatt agcgaaacga gctggacttt tacatgatgt
    16801 tggtaaagca attgatcatg aagtagaagg tagtcatgtt gaaatcggtg tagaattagc
    16861 gaaaaaatat ggtgaaaatg aaacagttat taatgcaatc cattctcatc atggtgatgt
    16921 tgaacctaca tctattatat ctatccttgt tgctgctgca gatgcattgt ctgcggctcg
    16981 tccaggtgca agaaaagaaa cattagagaa ttatattcgt cgattagaac gtttagaaac
    17041 gttatcagaa agttatgatg gtgtagaaaa agcatttgcg attcaggcag gtagagaaat
    17101 ccgagtgatt gtatctcctg aagaaattga tgatttaaaa tcttatcgat tggctagaga
    17161 tattaaaaat cagattgaag atgaattaca atatcctggt catatcaagg tgacagttgt
    17221 tcgagagact agagcagtag aatatgcgaa ataatttttg tctccctcac aaattagtga
    17281 gggagctttt ttaagttgta gtcttaatct agttagacag cactttatcg gtaataacta
    17341 tattaaacag tagttatttg aaagtaagac ggaccttata ttaaataaga agttattgct
    17401 tttaataaaa atgttttagg ct
//
LOCUS       DALAGF010000053        17277 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.053, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000053 DALAGF010000000
VERSION     DALAGF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17277
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.053"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(300..1298)
                     /gene="sirC"
                     /locus_tag="O2692_001606"
     CDS             complement(300..1298)
                     /gene="sirC"
                     /locus_tag="O2692_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HCZ4299239.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1295..2290)
                     /gene="sirB"
                     /locus_tag="O2692_001607"
     CDS             complement(1295..2290)
                     /gene="sirB"
                     /locus_tag="O2692_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HCZ4299240.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2306..3298)
                     /gene="sirA"
                     /locus_tag="O2692_001608"
     CDS             complement(2306..3298)
                     /gene="sirA"
                     /locus_tag="O2692_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HCZ4299241.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3529..4509
                     /gene="sbnA"
                     /locus_tag="O2692_001609"
     CDS             3529..4509
                     /gene="sbnA"
                     /locus_tag="O2692_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HCZ4299242.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4506..5516
                     /gene="sbnB"
                     /locus_tag="O2692_001610"
     CDS             4506..5516
                     /gene="sbnB"
                     /locus_tag="O2692_001610"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HCZ4299243.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5537..7291
                     /gene="sbnC"
                     /locus_tag="O2692_001611"
     CDS             5537..7291
                     /gene="sbnC"
                     /locus_tag="O2692_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HCZ4299244.1"
                     /translation="MQNHTAVNTAQAIILRDLVDALLFEDIAGIVSNSEITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRVSKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTVPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7284..8540
                     /gene="sbnD"
                     /locus_tag="O2692_001612"
     CDS             7284..8540
                     /gene="sbnD"
                     /locus_tag="O2692_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HCZ4299245.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8530..10266
                     /gene="sbnE"
                     /locus_tag="O2692_001613"
     CDS             8530..10266
                     /gene="sbnE"
                     /locus_tag="O2692_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HCZ4299246.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSKLSQVIEQSGLAPE
                     AWLECYLNRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            10175..12025
                     /gene="sbnF"
                     /locus_tag="O2692_001614"
     CDS             10175..12025
                     /gene="sbnF"
                     /locus_tag="O2692_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HCZ4299247.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEELQWQWVKGIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            12000..12776
                     /gene="sbnG"
                     /locus_tag="O2692_001615"
     CDS             12000..12776
                     /gene="sbnG"
                     /locus_tag="O2692_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HCZ4299248.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDEG"
     gene            12776..13978
                     /gene="sbnH"
                     /locus_tag="O2692_001616"
     CDS             12776..13978
                     /gene="sbnH"
                     /locus_tag="O2692_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HCZ4299249.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERKILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPKIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHRF
                     PLKHINLGGGIGVNYADLTNQFEWDNFVERFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13982..14746
                     /gene="sbnI"
                     /locus_tag="O2692_001617"
     CDS             13982..14746
                     /gene="sbnI"
                     /locus_tag="O2692_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HCZ4299250.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            14942..15349
                     /locus_tag="O2692_001618"
     CDS             14942..15349
                     /locus_tag="O2692_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299251.1"
                     /translation="MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFLSSILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
     gene            15399..16025
                     /locus_tag="O2692_001619"
     CDS             15399..16025
                     /locus_tag="O2692_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4299252.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVAAADTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEVKNPTTKNLEGFNEEIHKDEATTKVVSDNTEPPIESKDHVSKKD"
     gene            16237..17013
                     /locus_tag="O2692_001620"
     CDS             16237..17013
                     /locus_tag="O2692_001620"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HCZ4299253.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            17157..>17277
                     /locus_tag="O2692_001621"
     CDS             17157..>17277
                     /locus_tag="O2692_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299254.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQSYLILFL"
BASE COUNT         5816 a         2773 c         3417 g         5271 t
ORIGIN      
        1 ttacaattaa aaataatatg atatttttat taaaatttgt gtttttgaat aaatgacatt
       61 atcaaacact atatttaaat aagaaatagt tatcgatatt tgaaaaatcg aagaatattg
      121 attgatatta tagaatatga gtcggtgtta tttgtatata aagtattaat atgtaattaa
      181 gaaagtcgac gattgttaac aataacaaca actatatgta taaaaaaacc tcgcaacggt
      241 tagttaactg aatcatttag ctaacttcgt tgtgaggtca ttttgtttta ataatatcgt
      301 tataactttt tcacggttaa taataagtat atgaagaatg gggcaccaaa agcagcaata
      361 aatacacctg ctggcacttc tttaggcaag aataaggtac gcccaattaa gtctgcaata
      421 acaattgata tggcaccaat cattgctgac attagtaact ttttagcata acttccgcga
      481 acgattgttt tcgcgatatg tggtgcgatt aaaccgacaa acccaatgtt acctactaaa
      541 ctgattgcca tagatacgag tatagtagaa gtgattaatt ggattagttt catacgttgt
      601 acatgtaagc ctaagccaat cgctacaggg tcatcaagta tagatatttt catttttggt
      661 ataacaagaa ataacaacgg cacaacagct aaaataacca tacccaaaat gattgtatct
      721 ttaaacgtag caccgtaaag acttccgact agccatgtat aagctttggc agcagataat
      781 tgcttcgttg taatgagtaa tccttggaca agcgcaataa acaacgtttg catcgaaata
      841 ccgatgatta tgagtgttgt cgggcgtatt tgtcctttcg tttgaaacac taatagtatc
      901 atcattgcaa ctgcgccacc taatactgca aatagtggaa gtaaatgtat tgttaaatgg
      961 ctgaaaaatg caataaagac aacagcactt aagctagcac cacctgtgat accgataata
     1021 tcaggtgagg caattggatt ttttaataca ttttgcaaca ttaaaccact cattcctagt
     1081 gcggcacctg ctaaaatcgc aagtgtaatg cgaggtaagc gtaatacttc taaagtgaat
     1141 tgatccatac tgtcatttgg atttataaag tacatcagta cgcgttgtaa tggtataaag
     1201 cttgaaccaa tcatcatact taccactgaa acgatggcta aaaagattaa cgcgaagatg
     1261 agatggtaat tgtctttttt attaatcttt tcggtcataa gcgttgacgt cctttcttca
     1321 taatatagat taagacaata gcgccaatga cagcggtaac gacaccgata ggcaactcta
     1381 gtggcttaat tattatacga gcaacaatgt ctgaaatgat cattaggatt gctccagcta
     1441 atgcagtaaa aggaattaaa tacttatagt ttggtggtaa taatcgtttg ctaatattcg
     1501 gtacgataag acccacaaag acgattgatc cagctacggc taccgaaata ccggctaaca
     1561 tactgatgag cataataatc atccatttga ttaattttat gttttgaccg aggccggttg
     1621 caatgtcgtc acttgtcatc aagatgttga tgtgtgcagc catgctaaat gcaattaaaa
     1681 taagtatcaa tacaagcgga ataatccatg ggatatccca aatattacgt aatgaaacgg
     1741 agccacttaa ccaaaataat aggccttgta agtctgtttc gttcataata agtatgcctt
     1801 gagtaaaggc tgtaaatagc atcgcaatcg cagcacctgc caaaatgaca cggtgaggtg
     1861 agaatagtgt ttgtctaaac atacctagtg caacaactaa tacagtaaca acaatagccc
     1921 ccaaaaatgc aataactaca atcattttaa aagattgaat ttggataaat gtaatactaa
     1981 aaatgacaaa aaatactgcg cctgcattga caccgaaaag ccctggtgag gctattgggt
     2041 ttcgtgtaag tgcttgcatc aacaaacctg agacagcaag ggcagcacca gtcaataacg
     2101 caatgattgt tctcgacgcc cgtgcaccag tgacaacatc atgtaaatcg ttttcactat
     2161 caaagttgaa taacgcctgt atcaccgtac ctggtgacac aagcgtattt ccaatcatta
     2221 aacttaagat agctactatt gcaagacata aaccagcaat aacgatttgg tattttggtt
     2281 taagtagcat cgtaaaactc cttaattatt ttgattgttt ttcaatattt aacttttcat
     2341 ataaatcgtc aataagtttt aatgaagatt tatatccgcc agctaagttc caagtgattt
     2401 catctaaatc atcagatact tggttgtttt taactgcgtc taaatttttc cactctttac
     2461 ttgaagtcca ttcgctttca gtctttttaa ctaatgcagc atctttcgca tttggatctg
     2521 attttactac aaaaatatga tcagcgttca ttaatggaat gctttcttta gatgtaagtt
     2581 ggataatatc tttaccatta tcaacttgtt tttgtaagtc tttattacgt ttgaatccta
     2641 aatcatttaa gatttcacca gcatatccac cagcataaat tcttgtatga tcagcacgga
     2701 agttaacaac tgaagctttc aatggccatg catctttata ctttgctttt gcatcttttt
     2761 ggaatgcagc tactttatca tcgtactttt taagtaaatc ttcagcttct ttttctttcc
     2821 ctaaagcttt ccccattaac ttagttgtat ctttgaattt gaaaactgta tcagtagaaa
     2881 ctgttggtgc gattttagat aattgatcgt aaactttttc atttctaact tttgacgcga
     2941 caattaagtc cggttttaat ttagagattt cctctaagtt aggtgcaggt tcttgaccta
     3001 caatcttagt atcttttaaa tcatttttta tgtattcgaa tttcggtttt tgtgtccatg
     3061 attctacagc acctacaggt ttaacaccta aagatacagc gacgtcagtg gcaccttgat
     3121 atagcgtaac aacacgcttt ggtttccctt taatttcagt tgtacccatt gcatgtttaa
     3181 ttgaagttgt ttccttatct ttgttatcag atgattgttt atttgaattc ccactacatc
     3241 ctgctaaaac aagtaggaaa gcaagcgtaa caacaagcat tttaattact ttattcattg
     3301 actaattagc ctccttcgtg atgtatgaca atgagaatca ttatcacgat ttagtatgaa
     3361 ttaaattttt tcctaagtca ataaaatatt tatgatttac atgcaactta taattatttg
     3421 acatataaat gcataaaaaa tataatccta attacttgat agtgagaatc attatcaatt
     3481 aggtaacaca caatattata gaattttaaa tttgaggagg aagcgctttt gattgaaaaa
     3541 agtcaagcat gtcacgattc attgttagat tctgtagggc aaacacctat ggttcaactt
     3601 catcaactat ttccgaaaca tgaagtgttt gcaaagttag agtatatgaa tcctggaggc
     3661 agcatgaaag atcgacctgc caagtacatc attgaacatg gtattaaaca tggtttaatc
     3721 actgagaata cacatttaat tgaaagtact tctggtaatt taggcattgc gttggcaatg
     3781 atagctaaaa tcaagggatt aaaactcacg tgtgttgttg atcctaaaat atcaccaaca
     3841 aatttgaaaa ttattaaaag ttatggtgcc aatgtagaaa tggttgaaga acctgatgca
     3901 catgggggtt atttaatgac tcgtattgca aaggtgcaag aactgttagc cactattgac
     3961 gatgcatatt ggattaatca atatgcgaat gagttaaatt ggcaatccca ttatcatggt
     4021 gcaggcacag agattgttga aacaattaag caacctatag attattttgt cgcgccagtc
     4081 agcacgacag gtagcattat gggtatgagt agaaaaataa aagaagtgca tccaaacgca
     4141 caaattgttg ctgttgatgc gaaagggtca gtcatttttg gtgacaaacc tattaataga
     4201 gaattacctg gtatcggtgc tagtcgtgta cccgaaatat tgaatagatc agaaattaat
     4261 caagtgatcc atgtagatga ttatcaatct gctttgggct gtcgaaaact gattgattat
     4321 gaaggcatat ttgccggagg ttcaacaggt tcgattattg cagcgattga gcagttgata
     4381 acgtcaattg aagaaggtgc aacaattgtc acgattttac cagatcgagg cgatcgttac
     4441 ttagatttag tttattcaga tacatggtta gaaaaaatga aatcaagaca aggagttaaa
     4501 tcagaatgaa tagagagatg ttgtatttaa atagatcaga tattgaacaa gcgggaggta
     4561 atcattcaca agtttatgtg gacgcattaa cagaagcatt aacagcccat gcgcacaatg
     4621 attttgtaca accgcttaag ccgtatttaa gacaggatcc tgaaaatgga cacatcgcag
     4681 atcgaattat tgcaatgcca agtcatatcg gtggtgaaca cgcaatttca ggtattaagt
     4741 ggataggtag taagcacgac aatccatcga aacgtaatat ggagcgtgca agtggcgtca
     4801 ttattttgaa tgatccagaa acgaattatc caattgcagt tatggaagca agtttaatta
     4861 gtagtatgcg tactgcagca gtttcagtga ttgcagcaaa gcatttggct aaaaaaggat
     4921 ttaaagactt aacaatcatt ggatgcgggc taatcggaga caagcaatta caaagtatgt
     4981 tagagcaatt cgatcatatt gaacgcgtgt ttgtttacga tcaattctct gaagcatgtg
     5041 cacgctttgt tgatagatgg caacaacagc gtccggaaat taattttatt gcgacagaaa
     5101 atgctaaaga agcagtatca aatggtgaag tagtcattac atgtaccgta acggatcaac
     5161 catacattga atatgattgg ttacaaaagg gtgcatttat tagcaacatt tctatcatgg
     5221 atgtgcataa agaagtcttt attaaagctg acaaagtcgt agtagatgac tggtcacaat
     5281 gtaatcgaga aaagaaaact attaaccaat tggtgttaga aggtaaattc agcaaagaag
     5341 ctcttcatgc tgaactagga caacttgtga caggtgacat accaggacgt gaagacgatg
     5401 atgagatcat attacttaat ccgatgggta tggctatcga agatatttca agtgcttatt
     5461 ttatttatca acaggcacaa caacaaaata ttgggacaac attgaaccta tattaagaat
     5521 gcgaggtgtc tgaacattgc agaatcatac agcagtcaat acagcacaag cgataatatt
     5581 aagagattta gttgatgcat tattatttga agatatagcc ggaattgtat cgaatagtga
     5641 gattactaaa gaaaacggac aaacgctttt gatatacgaa cgtgaaacac aacaaataaa
     5701 gatacctgtt tattttagtg ctttaaatat gtttcgttac gaaagttcac aaccaattac
     5761 gatagaggga agggtgtcta agcaaccttt aacggcagct gaattttggc aaacaattgc
     5821 taatatgaat tgtgatctaa gtcatgaatg ggaagtggct cgcgttgaag aaggactgac
     5881 tactgctgcc acacagcttg ctaaacaatt atcagaatta gatttagcgt cacatccttt
     5941 tgtgatgtca gagcagtttg caagtttaaa agatcgtcca tttcatccat tagctaaaga
     6001 aaaaagagga ttaagagaag cggattatca agtgtatcaa gctgaattaa atcaatcatt
     6061 tcctttaatg gttgcagcag ttaaaaagac acatatgatt catggcgata ctgcaaatat
     6121 cgatgaatta gaaaatttga cagtacctat aaaagaacaa gcgacagaca tgttaaatga
     6181 tcaagggtta tcaatagatg actatgtact atttccggta catccttggc aatatcagca
     6241 tattctgccg aacgtctttg cgaaagagat tagtgaaaag ttggttgtac tattaccgtt
     6301 aaaatttgga gattatctgt cgtcttcaag tatgcgttca ttaattgata ttggcgcacc
     6361 gtataaccat gtcaaagtac catttgcaat gcagtcatta ggcgcattaa ggctaacgcc
     6421 tacgcgttac atgaaaaacg gagaacaagc agaacaatta ttacgtcagc ttatagaaaa
     6481 agatgaagca ctagctaagt atgtcatggt ttgtgatgaa acagcttggt ggtcatatat
     6541 gggtcaagat aatgatattt tcaaagatca attaggtcat ctaactgttc agctaagaaa
     6601 gtatcccgaa gtgctagcca aaaatgatac gcaacagcta gtgtcaatgg cagcactcgc
     6661 ggcaaatgat cgcactttat atcaaatgat ttgtggaaaa gataatattt ctaaaaatga
     6721 tgtcatgacg ttatttgaag atatcgcgca agtcttttta aaggtaacac tatcatttat
     6781 gcaatacggc gcattaccag agttgcatgg tcaaaatata ttgttgtcat ttgaagatgg
     6841 acgtgtacaa aaatgcgtgt tacgtgatca tgatactgtc agaatttata aaccatggct
     6901 aacagcacat cagctttcat tgccgaagta tgtcgtcaga gaagatacac ctaatacgct
     6961 aattaatgag gatttggaaa cattctttgc ttattttcaa acattagctg tatcggtaaa
     7021 tctatatgcc attattgatg caattcaaga tttatttggt gtaagtgagc atgaacttat
     7081 gtcgttgtta aaacaaattt taaaaaatga agtggcaact atttcctggg ttacaactga
     7141 tcagctagct gtcagacaca ttttatttga taaacagacg tggccattca aacaaatttt
     7201 attaccattg ctatatcaac gtgatagtgg tggaggtagt atgccttcag gtttaactac
     7261 cgtaccaaat ccaatggtga catatgatta atcagtctat atggcgcagt aactttcgca
     7321 ttttatggct cagtcagttt atagcgattg ctggactgac agtacttgtg ccattattgc
     7381 caatttatat ggcatcacta caaaatctat cagtcgtaga aatacagttg tggagtggta
     7441 tagcgattgc tgctccagct gtaacgacga tgatagcttc gccgatatgg gggaagctag
     7501 gtgataagat cagccgaaaa tggatggtgt taagagcgtt acttggtttg gcggtatgct
     7561 tatttttaat ggcattgtgt acgacaccat tacagtttgt acttgtgagg ttattgcagg
     7621 gactatttgg tggtgttgtt gatgcatcaa gtgcgtttgc gagtgcagag gcgccagctg
     7681 aagatcgtgg aaaggtatta ggaagactgc aaagttcagt cagcgcaggg tctcttgtgg
     7741 ggccattaat tggcggtgtt acagcttcga tattaggttt tagtgcgtta ctgatgagta
     7801 ttgccgttat tacttttatt gtctgtattt tcggtgcatt aaaattgatt gaaacgacac
     7861 atatgccaaa atcacaaaca ccaaatatta ataaaggtat tcgccgttca tttcaatgtc
     7921 tattatgcac acaacaaaca tgtcgattta ttatcgttgg cgttttagca aactttgcta
     7981 tgtatggcat gctaactgca ttatcaccac ttgcttcatc agtgaatcat acagcgatag
     8041 atgaccgtag tgtgattgga tttttacagt ccgcattttg gacggcttcg atattaagcg
     8101 cgcctttatg gggacgcttt aatgataaat catatgttaa atcagtatat atatttgcca
     8161 cgattgcatg tggttgtagt gcgatactgc aaggtttagc gacgaatata gagtttttaa
     8221 tggctgcaag aatacttcaa ggattaacat atagtgcatt gattcaaagt gtcatgtttg
     8281 ttgtcgtgaa tgcgtgtcat caacaactta aaggcacatt tgttggaacg acgaacagta
     8341 tgttagttgt tggtcaaatt attggcagtc ttagtggcgc tgccattaca agttatacta
     8401 caccagctac tacgtttatc gttatgggcg tagtatttgc agtaagtagt ttatttttaa
     8461 tttgttcaac catcactaat caaatcaacg atcacacatt aatgaaatta tgggagttga
     8521 aacaaaaaag tgcaaaataa agaattaata caacatgcag cgtatgcggc tatcgaacgc
     8581 attttaaatg aatattttag agaagaaaat ttatatcaag taccacctca aaatcatcaa
     8641 tggtctatac aattatcaga gctcgaaact ttaacgggtg aatttcgcta ttggtctgcg
     8701 atggggcatc atatgtatca tccagaggta tggcttatcg atggaaaaag taaaaaaata
     8761 acaacttata aagaagcaat tgcgcgtatt ttgcaacata tggctcaaag tgcagataat
     8821 caaacggcag tgcaacaaca tatggcgcaa attatgtctg acatcgataa tagcattcat
     8881 cgcacggcac gttatttgca aagtaacaca atagactacg tagaggatcg ttatatcgtt
     8941 tcagaacaat ctttatactt aggtcatcca tttcatccga ctcctaagag tgcaagtggg
     9001 ttttcagaag cagatttaga gaaatatgca cccgaatgtc atacatcatt ccaattgcat
     9061 tatttagctg tgcatcaaga tgttctgctc acgcgctatg tagaaggtaa agaagatcag
     9121 gttgagaaag tgttgtatca attagcagac atagatatat cagagatacc caaagatttt
     9181 attttattac caacacatcc ttatcaaatc aatgtgttgc gacagcatcc acagtatatg
     9241 caatatagtg aacaaggttt aataaaagac cttggcgttt ccggtgattc agtgtacccg
     9301 acgtcttcgg ttagaactgt attttcaaaa gcattaaaca tttatttaaa attaccgata
     9361 cacgttaaaa tcactaattt tatacgtacg aatgaccttg aacagattga acggacaatt
     9421 gatgccgcgc aagttatcgc atcagtcaaa gatgaggttg aaacacccca ttttaaattg
     9481 atgtttgaag aaggatatcg tgcattgtta ccgaatccat tagggcaaac agttgaacct
     9541 gaaatggatt tattaacaaa tagtgccatg attgttcgtg aagggatacc gaattaccat
     9601 gctgataaag atattcatgt attggcgtca ttatttgaaa cgatgcctga ttcaccgatg
     9661 tctaagttat cacaagtgat tgagcaaagt ggtttagcac cagaagcatg gcttgaatgt
     9721 tatttgaatc gtacattatt gccgatatta aagctgttta gtaacacagg cattagtcta
     9781 gaagcacatg tacaaaatac attaattgaa ttaaaagatg gcatacccga cgtatgcttt
     9841 gtcagagatc ttgaaggcat ttgtctatct agaacgattg ctactgaaaa acagcttgtg
     9901 ccaaatgttg tggcagcatc aagccctgtt gtatatgcac atgatgaagc atggcatcgt
     9961 cttaaatatt acgttgtagt aaatcactta ggacatttag tatcaactat tggtaaagcg
    10021 actagaaatg aagttgtgtt atggcaactt gtagcgcatc gtcttatgac ttggaaaaaa
    10081 gaatacgcga ataacgcagt atttgttgac tgtgtagaag atttatatca aacgccgacc
    10141 attgcggcta aagcgaattt gatgagtaaa ttgaatgatt gtggtgcaaa ccctatttat
    10201 acacatatac caaatccaat ttgtcataac aaggaggtat cgtattgtga atcaaacaat
    10261 tcttaatcgt gtaaagacta gagtgatgca ccaactggta tcatcactta tttatgagaa
    10321 tattgttgtg tataaagcgt catatcaaga cggtgtcggt cattttacaa tagaaggaca
    10381 tgattcagag tatcgtttta ctgctgaaaa gacacatagc tttgatcgta tacgtatcac
    10441 atcaccaatt gagcgtgtcg taggagatga ggcagataca acaacagact atacacaatt
    10501 attgagagag gttgtattta catttcctaa aaatgatgaa aagctagaac aatttattgt
    10561 cgagttatta cagacagaat taaaagatac gcaaagtatg cagtatcgag aatcaaaccc
    10621 accagcaaca cctgagacat ttaacgacta tgaattttat gcgatggaag ggcatcagta
    10681 tcatccaagt tacaaatcac gtttaggatt tacgttgagt gataatttga aatttggtcc
    10741 tgattttgta ccaaacgtta aactgcagtg gttagctatc gacaaagata aagtagaaac
    10801 gacggtatca agaaatgttg tagttaacga aatgttacgt caacaagttg gcgataagac
    10861 ttatgaacat tttgtacagc agattgaagc atctggcaaa catgtaaatg atgttgagat
    10921 gatacctgta cacccatggc agtttgaaca tgtcatccaa gttgatttgg ctgaagaaag
    10981 gcttaatggc acagtactat ggttagggga aagtgatgag ctatatcacc ctcaacaatc
    11041 gattcgtacg atgtcgccaa tagacacgac aaaatattat ttaaaggtac caataagtat
    11101 aacgaacact tcaacgaaac gagtgttggc gcctcataca attgaaaatg cagcgcaaat
    11161 tacggattgg ttaaagcaga tacagcaaca agatatgtat ttaaaagatg aattaaagac
    11221 agtttttcta ggggaagtct taggacagtc ttatttaaat acacaacttt cgccttataa
    11281 acaaactcaa gtttatggtg cgttaggtgt tatatggcgt gaaaatatat atcatatgtt
    11341 aatcgatgaa gaggatgcga taccatttaa tgcactttat gcaagtgata aggatggtgt
    11401 accattcatt gaaaattgga ttaaacaata tggttctgaa gcttggacaa agcaattttt
    11461 agctgtagcg attcgtccaa tgattcatat gctttattat cacggtattg cctttgaatc
    11521 gcatgcacaa aatatgatgc tcattcatga aaatggttgg cctacacgta ttgccttaaa
    11581 agatttccat gatggtgttc gttttaagcg tgagcattta agtgaagctg cttcacacct
    11641 gacattaaag ccaatgccag aagcacataa aaaagtgaat agtaattcat ttattgaaac
    11701 agatgacgaa cgtttagtac gcgacttttt acatgatgca tttttcttta ttaatatcgc
    11761 cgaaatcatc ttatttattg aaaagcaata tggtatcgat gaggagctgc aatggcaatg
    11821 ggttaaaggc atcatcgagg cgtatcaaga agcatttcca gagttgaata actatcaaca
    11881 tttcgatttg tttgaaccta cgattcaagt tgaaaagtta acgacacgtc gattattaag
    11941 tgactccgag ttaagaattc atcatgttac aaatccatta ggtgtaggag gtatcaatga
    12001 tgcaacaact atctctgaaa catagattaa acaatggtga ttcagtttat ggcattttta
    12061 attctatacc ggacccattg atgatcgagg ttatcgcagc aagcgggtat gactttgttg
    12121 tgattgatac agaacacgtg gcgattaatg atgagacact agcgcattta attcgtgcag
    12181 ctgaagcagc gcatattata ccaattgtac gtgtcactgc agtgatagat agagatatca
    12241 ttaaagtgtt ggatatgggt gcgagaggta ttattgtgcc acacgttaaa gatcgtgaga
    12301 cagttgagca tattgtgaaa ttaagtcgtt attacccgca aggattaaga agtttgaatg
    12361 gtggtcgcat ggcaagattt ggacgtacac cattacttga tgcaatggag atggctaatg
    12421 agcatattat ggtgattgcc atgatagaag atgttgaagg ggttatggcc attgacgata
    12481 tagcacaagt cgaaggttta gacatgatag tcgaaggtgc cgcagattta tcgcagtcac
    12541 ttggcatacc atggcaaacg cgtgatgatc aagtaacatc acatgttcaa catatttttg
    12601 aagttgtgaa tgcacatggt aaacattttt gtgcattacc acgtgaagat gaagatattg
    12661 caaaatggca ggcacaaggt gtacaaacat ttattttagg tgatgatcgc ggaaaaatat
    12721 atcgccattt aagtgcatct ctagcgacgt ctaaacagaa aggggatgaa ggctaatgcg
    12781 tatagttcaa cctgttattg aacaattaaa agcacaatct catccagttt gtcattatat
    12841 ctatgattta gtcggactgg aacatcattt gcaacatatt acatcgtcat tgccgagtaa
    12901 ttgtcaaatg tactatgcaa tgaaagcaaa tagtgaacga aaaatcctag atacaattag
    12961 tcagtatgtt gaaggattcg aagttgcatc tcaaggtgaa atagcaaaag gtcttgcttt
    13021 taaaccagca aatcatatta tttttggtgg ccctggtaag acagacgagg aactaagata
    13081 tgcagtaagt gaaggtgttc agcgtattca tgttgaaagt atgcatgaat tacaacggct
    13141 aaatgccatc ttagaagatg aagataagac acaacacatt ttattgcgtg ttaatttagc
    13201 aggaccattt cccaatgcaa cgttgcatat ggcaggacgc ccaacacaat ttggtatttc
    13261 tgaagacgaa gttgatgatg tcattgaagc tgcgctcgca atgccaaaga ttcatctaga
    13321 tggatttcat tttcattcta tttctaacaa tttagactcg aatttacatg tcgatgtagt
    13381 gaaactttat tttaaaaaag caaaggcatg gtctgaaaaa catcgatttc cactcaaaca
    13441 tatcaatctt ggtggtggca taggcgttaa ctatgcagat ttaactaacc aatttgaatg
    13501 ggataatttt gtagaacgtt ttaaaacact tatcgttgag caagaaatgg aagatgtgac
    13561 attgaacttt gaatgtgggc gctttattgt ggcacatatt ggttactatg tgacagaagt
    13621 gctagacatt aagaaagtac atggtgcttg gtatgccata ttaagaggag gtacgcaaca
    13681 atttagactg ccggtatctt ggcaacataa ccatcctttt gacatttatc gctataagga
    13741 caatccatat tcatttgaaa aagtttcaat ttcgagacag gacacaacgt tagtcggtca
    13801 attatgtaca ccgaaagatg tctttgctag agaagtacag atagacgcaa tcagtacagg
    13861 cgacgttatt gttttcaaat atgcaggtgc atacggatgg tctatttcac atcacgattt
    13921 cttaagccat ccacatcctg aatttattta tttaacgcaa acaaaggagg atgaataact
    13981 attgaatcat attcatgaac atttaaaatt ggtaccagta gataagattg atcttcacga
    14041 aacattcgaa cctttaagat tggaaaaaac gaaaagtagt attgaagcag atgattttat
    14101 acgtcatcct attttagtga cagcgatgca acatggtaga tatatggtta tagatggtgt
    14161 gcatcggtat acaagtttga aagcgttagg atgtaagaaa gttccagtgc aagaaatcca
    14221 tgaaacacaa tattcaatta gtacatggca acataaagtt ccatttggtg tgtggtggga
    14281 aacgttacaa caagaacatc gcttgccatg gactactgag acaagacaag aagcgccatt
    14341 tattacaatg tgtcatggtg atacagaaca atatttgtat acaaaagatt taggcgaagc
    14401 acattttcaa gtatgggaaa aggttgtcgc aagttatagt ggttgttgtt ctgtagagag
    14461 aattgcacaa ggtacatatc cttgtctttc tcaacaagat gtactcatga agtatcagcc
    14521 attgagttat aaggaaattg aagcggttgt tcataaaggg gaaactgtgc cagcaggtgt
    14581 gacacgcttt aatatttcag gacgatgtct taatcttcaa gtaccactgg cattacttaa
    14641 acaagatgat gatgttgaac aactgcgcaa ttggaagcag tttttagcag ataagtttgc
    14701 caatatgaga tgctatactg aaaaagtata cttggtggag caatagtttt actgtgatgt
    14761 tgagggaaat atgatgattt agcgtattga tagcgaaaat ataataaaac aatatagtgt
    14821 ggagaacttt tgatatttta taaatattga agttctccat ttttgtattt tgcatataaa
    14881 aattaaataa aataaggtat attaaggtaa agtataaatt ttaaataaat ggggaatgag
    14941 tatgagctca attataggaa aaatagcaat ttggataggc atcgtagctc aaatatattt
    15001 tagtgtcgtt tttgttagga tgatatctat taatattgct ggaggatctg attacgaaac
    15061 aattttttta ttaggattaa tattggctct tttcactgtt ttaccaacca tctttactgc
    15121 gatttatatg gaaagttact ctgtaatcgg aggtgcactt tttattgttt atgctattat
    15181 tgcactgtgt ttatataatt tcctttcgtc aattttatgg ctgattggtg gtattttgct
    15241 gatttggaat aaatactcaa aagatgaatc gacagacgaa aatgaaaaag ttgatattga
    15301 aagtacagag aatcaatttg aatctaaaga taaaatcact aaagaataaa gagaatattt
    15361 aaggtaaagt ataaatttta aataaatggg gaatagacat ggaaaaaaat gtagaaaaat
    15421 cattcataaa gataggttta tattttcaaa tagcttatat agtactcatg gctataactt
    15481 tatgtgggtt tgtaatttgc tatggactaa ttttcggcct tttctattta ttatcaggta
    15541 gcagagctga ttatttaata gtaacaatag ttatatcggc aataatttct atatttgtaa
    15601 ttatactttc aatcgtacct gtcatcgtat tggcatctga cttatttaaa gaaaggattt
    15661 caaaaggtgt catattaatt gtattggcta ttatcgcttt agtattatgc aactttgtat
    15721 ctgcaatact ctggtttgtt tcagccatat ctattttagg tagaaaaaaa ttagtagctg
    15781 cagcagatac taccactatt caaaaaagta aagggaacgc aaatcaagca tcacataaag
    15841 acacgtgtaa aaaggaactt gatagtcaag acatgatgga acatcctgag gttaaaaatc
    15901 ccacgactaa aaaccttgaa ggatttaacg aagaaataca taaagatgaa gctacaacta
    15961 aagttgtcag tgataacacg gaaccgccta ttgaatcaaa agaccatgtc tcgaaaaaag
    16021 attgatgaca aactaatcga gagacttaaa aaaataatat tcaacataag aacttttaaa
    16081 acgacattta aacgcattgc caatcactaa tggtagtgcg tttaactata ccttaaatat
    16141 ctgaatattt tgttaaatgg agctaccttt gttgtactat tcaaatgaag aggagtaaaa
    16201 tgtaattaaa ggaaagaaat ttgaggagtg atctttatga caaacaacaa agtagcatta
    16261 gtaactggcg gagcacaagg gattggtttt aaaattgcag aacgtttagt ggaagatggt
    16321 ttcaaagtag cagttgttga tttcaatgaa gaaggggcaa aagcagctgc acttaaatta
    16381 tcaagtgatg gtacaaaagc tattgctatc aaagcagatg tatcaaaccg tgatgatgta
    16441 tttaacgcag taagacaaac tgccgcgcaa tttggcgatt tccatgtcat ggttaacaat
    16501 gccggccttg gaccaacaac accaatcgat acaattactg aagaacagtt taaaacagta
    16561 tatggcgtga acgttgcagg tgtgctatgg ggtattcaag ccgcacatga acaatttaaa
    16621 aaattcaatc atggcggtaa aattatcaat gcaacatctc aagcaggcgt tgagggtaac
    16681 ccaggcttgt ctttatattg cagtacaaaa ttcgcagtgc gaggtttaac acaagtagcc
    16741 gcacaagatt tagcgtctga aggtattact gtgaatgcat tcgcacctgg tatcgttcaa
    16801 acaccaatga tggaaagtat cgcagtggca acagccgaag aagcaggtaa acctgaagca
    16861 tggggttggg aacaatttac aagtcagatt gctttgggca gagtttctca accagaagat
    16921 gtttcaaatg tagtgagctt cttagctggt aaagactctg attacattac tggacaaaca
    16981 attattgtag atggtggtat gagattccgt taataatcat ccactaatga taaataaatc
    17041 cttattgtta agtttaatca cttagcagta aggatttttt agtgcactta gaagggagtg
    17101 tattggtaga aaattaataa gcgaagttct taagtgagtt atgatgtcac agtctaatgc
    17161 atcagttgaa agcattatta gtattaacac acccaagata ttataaaaca tcacaaaaac
    17221 accactatct aatttatctc aataaaaatt cacaaagtta tctcatttta tttttat
//
LOCUS       DALAGF010000054        17215 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.054, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000054 DALAGF010000000
VERSION     DALAGF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17215
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.054"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(185..589)
                     /locus_tag="O2692_001622"
     CDS             complement(185..589)
                     /locus_tag="O2692_001622"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCZ4299255.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            complement(609..2021)
                     /gene="atpD"
                     /locus_tag="O2692_001623"
     CDS             complement(609..2021)
                     /gene="atpD"
                     /locus_tag="O2692_001623"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCZ4299256.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            complement(2043..2909)
                     /locus_tag="O2692_001624"
     CDS             complement(2043..2909)
                     /locus_tag="O2692_001624"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCZ4299257.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            complement(2940..4448)
                     /locus_tag="O2692_001625"
     CDS             complement(2940..4448)
                     /locus_tag="O2692_001625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCZ4299258.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            complement(4470..5009)
                     /locus_tag="O2692_001626"
     CDS             complement(4470..5009)
                     /locus_tag="O2692_001626"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCZ4299259.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLR
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            complement(5009..5530)
                     /locus_tag="O2692_001627"
     CDS             complement(5009..5530)
                     /locus_tag="O2692_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCZ4299260.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            complement(5728..5940)
                     /gene="atpE"
                     /locus_tag="O2692_001628"
     CDS             complement(5728..5940)
                     /gene="atpE"
                     /locus_tag="O2692_001628"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCZ4299261.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            complement(5983..6711)
                     /gene="atpB"
                     /locus_tag="O2692_001629"
     CDS             complement(5983..6711)
                     /gene="atpB"
                     /locus_tag="O2692_001629"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCZ4299262.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            complement(6732..7184)
                     /locus_tag="O2692_001630"
     CDS             complement(6732..7184)
                     /locus_tag="O2692_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HCZ4299263.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLIIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            complement(7252..8379)
                     /gene="wecB"
                     /locus_tag="O2692_001631"
     CDS             complement(7252..8379)
                     /gene="wecB"
                     /locus_tag="O2692_001631"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCZ4299264.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            complement(8403..9032)
                     /gene="upp"
                     /locus_tag="O2692_001632"
     CDS             complement(8403..9032)
                     /gene="upp"
                     /locus_tag="O2692_001632"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCZ4299265.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            complement(9060..10298)
                     /locus_tag="O2692_001633"
     CDS             complement(9060..10298)
                     /locus_tag="O2692_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCZ4299266.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALGMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            complement(10325..10849)
                     /locus_tag="O2692_001634"
     CDS             complement(10325..10849)
                     /locus_tag="O2692_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCZ4299267.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            complement(10956..11375)
                     /locus_tag="O2692_001635"
     CDS             complement(10956..11375)
                     /locus_tag="O2692_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HCZ4299268.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            complement(11372..12418)
                     /locus_tag="O2692_001636"
     CDS             complement(11372..12418)
                     /locus_tag="O2692_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCZ4299269.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKLGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            complement(12502..13338)
                     /gene="prmC"
                     /locus_tag="O2692_001637"
     CDS             complement(12502..13338)
                     /gene="prmC"
                     /locus_tag="O2692_001637"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCZ4299270.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            complement(13325..14401)
                     /gene="prfA"
                     /locus_tag="O2692_001638"
     CDS             complement(13325..14401)
                     /gene="prfA"
                     /locus_tag="O2692_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCZ4299271.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            complement(14402..15001)
                     /locus_tag="O2692_001639"
     CDS             complement(14402..15001)
                     /locus_tag="O2692_001639"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCZ4299272.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            complement(15348..15602)
                     /locus_tag="O2692_001640"
     CDS             complement(15348..15602)
                     /locus_tag="O2692_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCZ4299273.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            complement(15720..17036)
                     /gene="rho"
                     /locus_tag="O2692_001641"
     CDS             complement(15720..17036)
                     /gene="rho"
                     /locus_tag="O2692_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCZ4299274.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
BASE COUNT         5073 a         3475 c         2581 g         6086 t
ORIGIN      
        1 tttactcatg tattcctatt tttaaataca cattagctgt gtttaatgat aaagaaccac
       61 tacatcataa atctttaatg gttctttatc aattccttcc cccctctcat gttttgtatc
      121 tttaaatcaa tataattcaa aaaagggtcg aagatatgaa atcacatctt cgacccttta
      181 tttactattt taattcagcc acacgcaaac ggttatttgc tctctctaaa gctctttcgg
      241 ctctatgaat atcagtattg tcgtcatcat tttccaagtg agactctgct cttgctttgg
      301 ctaatttagc tctttcaaca tcaatttctc ttgcagtttc tgcagtctga acaatgattg
      361 aaactttatc ttttctaact tcaacaaagc catcgcttac agcaatatat tcagttccat
      421 cgtgaaattt cacttttaca aagcctgttt ttaaagcagc tacagttgga atatgtccac
      481 tcatgacacc tatctcacca gctgttgttt gcataacaac gagttcaaca ttatcacgat
      541 tgtaaacaga accattagga gtgacaatat ctaggtttaa tgtattcatt atccattcct
      601 cctaattgtt atacttcaac acccatatct ttagcttttg caataacatc atccatgcta
      661 ccaactaaac ggaatgcatc ttctggaata tggtcatatt taccatctaa gatatcttta
      721 aagtttgcaa ctgttgtctt aacaggtaca taagaacctt tttgaccagt aaattgttcc
      781 gctacgtgga agttttgaga taagaagaat tgaattctac gtgcgcgttc aactgtttgt
      841 ttatcttcat cagataattc gtccatacct aagatagcaa tgatatcttg taattcacgg
      901 tatttttgaa gtgttgattg tacatcacga gctacttcat aatgttcttg acctacaatt
      961 gatggttcca atgctcttga tgtagacgct aatggatcca cggctggata aatacccatt
     1021 tcagttaatt tacgttctaa gtttgtagtt gcatctaaat gggcaaacgc tgtcgcaggc
     1081 gctgggtcag tatagtcatc ggcaggtacg aataccgctt gaatagaagt aactgatcct
     1141 tttgttgtag acgtaatacg ttcttgtaat tgtcccattt cagtagcaag tgttggttgg
     1201 taacctactg cagaaggcat acgacctaat aatgcagata cctcagaacc agcttgtgta
     1261 aatctgaaaa tgttatcgat gaataataat acgtcttgac cttgttcgtc acggaaatat
     1321 tcagccattg ttaaaccaga taatgcaaca cgcatacgtg caccaggtgg ctcattcatt
     1381 tgcccgaata ccatggctgt tttcttaatt acaccactgt cactcatttc gaagtataaa
     1441 tcgttacctt cacgagtacg ttcacctaca ccggcgaata cagaaatacc accgtgctct
     1501 tgagcgatgt tgttaattaa ttcttggatt aatactgttt tacctacacc ggcaccaccg
     1561 aacaatccga ttttaccacc tttaatataa ggtgctagta aatctactac tttaatacct
     1621 gtttctaaaa tttgaacttc tgttgaaagt tcatcgaatg ctggtgcttg acgatggata
     1681 ggatcgcggc gaacagaatc actaatttct tctttaaggt caattgtttc acctagtaca
     1741 ttaaatacac gacctaatgt ttcgtcacca acaggtacac taatttcttt gcctgtatct
     1801 tttacatcca tgcctctttg gacaccatca gttgaatcca tcgcaattgt acgaacaacg
     1861 tcgtcaccta attgcagcgc aacttctaat gttagttgta ttgtaccttc ttctttaggc
     1921 acatcaataa ccaaggcgtt attaatttta ggaacttcgt tatgttcaaa tcgaacatca
     1981 attacaggac ccataacttg agttacacgg ccaattccca tgctattttc ctcctttaaa
     2041 tattattcaa gcgctgcgga accaccaaca atttcagtaa tttgttgcgt aatttctgct
     2101 tgtctcgctc tgttatattc taatgataag tcatcaataa gttcagttgc attatcagtg
     2161 gcatttttca tcgcagtcat acgtgttgca tgctcacttg cttttgcgtc taatattgtt
     2221 ccgtaaatca aactctcaac atattgaggc aagattacac ttaagataga ttctttatct
     2281 ggctcaaatt cataagaaga caaatgacca tgccccttac tagaatcctc ttgagataat
     2341 ggtaatactt gtctagatgt aggcttgttt tcaagaacgc tgacataatg actatagtat
     2401 atatttaatt catcaatttc ttcttcactg tataagtcta tagcatggtt agctagtgct
     2461 tgaacagatt tgaaagaagg ttgatcaggt acgtctactt gagaatactc aatgtcataa
     2521 cctctatttt ttaggaaatc aacaccttgt tgccctaaaa ctacaatact gtattcgcta
     2581 ctatcttgat gtttcgcttc aatatcagta atcaattttt taagcacgtt tgcactatat
     2641 gcacctgcta aacctttatc actcgtgata actaaatatc cacttctagt aattttacga
     2701 ggtcttagca ttggatggtt tgtattgcta cttgcccctg caactgcagt aattgcatct
     2761 tgcattttat ccatatatgg tgtgaattgt tttgtatttt tttcagctct acgaagtttt
     2821 gaacttgata ccatgttcat cgctttcgta atctgcttca tttttttggt tgattttatt
     2881 cgagtatcta tttctttaag agaagccact atctcaccac cttatactaa acttgtttat
     2941 tattcagatt tgctaaagct ttttttgaat tcgttaattg ctgtgtcaaa cttctcagca
     3001 tctggtaagc caccagtttc tctgatttca tttaacagtt cagtagcatt tgattctgcc
     3061 cagtggttta actcgtcttc aaaacgtgtg atatctacaa ctggaatatc atctaaatat
     3121 ccttttgtta atgcataaat aatcaacact tggtgttcga ctggtaatgg tttgttttga
     3181 tcttgtttta agacttcaac agtacgttta ccacgttcta atttacttgc agtaaattca
     3241 tcaaggtctg aaccgaattg tgcaaatgat tcaagttctc tgtatgacgc taagtcaaga
     3301 cgtaacgtac cagcaacttt cttcattgct ttaatttgtg cagatccacc aacacgagat
     3361 acagattgtc cggcattaat cgctggtctt acacctgaga agaataaatc agattgtaag
     3421 aagatttgtc catctgtaat tgaaataacg tttgttggta cataagctga aatatcacca
     3481 gcttgtgttt cgataattgg taatgcagta attgaaccgc cacctaagtc atcgtttaat
     3541 tttgctgctc tttctaataa tctactatgt aagtagaata cgtcacctgg gtatgcttca
     3601 cgacctggag gtctacgtaa taataatgat aactcacgat aagctgctgc ttgtttagtt
     3661 aaatcatcat aaacgattaa aacatgttta ccgttaaaca tgaattcttc acccattgtt
     3721 acacctgaat atggtgcaat atataataat ggagaaggtt cagaagctga tgctgctaca
     3781 acaatagtgt agtctaaagc gcctgcttgt cttaactttt caacatttgc tcttactgtt
     3841 gaatcttttt gaccaatagc aacatagata caaatcgtac cttgatcttt ttggttcaaa
     3901 attgtgtcaa ttgcaattgt tgttttacct gtttgacggt caccgatgat taactcacgt
     3961 tgacctctac caattggtac taaagcatca attgctttga tacctgtttg taatggctca
     4021 tctactgatt tacgatccat tacaccagta gcttttttct ctactggacg tgttttagtt
     4081 gtgttaatcg gtccttgtcc atcaataggt tgtcctaatg gattaacaac tcttccgatt
     4141 agttcttcac ctactggtac ttccatgata cgaccagtac gtttaacttc gtcaccttca
     4201 gtaatacctg tgtatggtcc taaaataacc acacccacgt ttgactcttc aaggttttgg
     4261 gctaaaccaa gtacgccgtt atggaattct actagctcac cagccataac gtcatttaat
     4321 ccgtgaatta atgcaatacc atcaccaatt tgtaatacag tacctacatc agttacggac
     4381 atttctgact cataattttc aatttgtgag cgaagtaatg cactgatttc ttcagctttt
     4441 atggccatct atgtcactcc tctttataat taattaactc ttctaaattt tctttgtaat
     4501 tggacaagat catttctaac actaccatct aatacagttg tgccgacttt aactctaaat
     4561 ccaccaatta aatctggatt aatttttgta tctacaataa ctttagataa cttcgtttgt
     4621 tgagttacta gtttgacaat cttatctaac tcttcttgac tcaattcata tgttgactca
     4681 attgttgcaa aatcttgatt gtagtgtccg ttatataagc tttggaacgc cttgaatacg
     4741 tcagctatta atgagatatg tctattatct gctaacacgt acatcatatt tttaatgtat
     4801 ggattaatgt cagtaaatac accattaatc aactcacgac gttgttctgc tgtttgagtt
     4861 ggattactgt caaccattct caattgctca attttatctt ttactgcttc atttataact
     4921 gttaattctt cattaatagt ctctaaatta tttgtatcta atgacacgtc aaataatgct
     4981 ttagcatact tgttagctac ttttaccatt atttatcgcc tgcctctttt agatacttgt
     5041 caaccaatgc tttttggtct tgttcagaaa tttcttttct aagaacttta gaagcaatta
     5101 acactgatag ttcagatact tgattattaa tatctgcaat ggcacgttct ttttggctat
     5161 tgatttcact ttgtgctgtt tcaatcatac cgtttgcacg tacgtttgct tcatgaataa
     5221 tttgttcttg ctgttgacgt gcttgaacct tagcatcttc taaaatcttt tgaacttctt
     5281 cttgtgtttc tttaagtttt tgtttatttt cttcttcaag tttctgtgca tttaacttag
     5341 cttgttctgc gtcatcgata tctctgttaa tatctctttc acgtttatcc attacatctt
     5401 tcaatggacc ccatgcgaac tttttaagta acgcaagtaa cacgatgaaa gttaggacct
     5461 gtacaatcac agtaccccac tcaacgcctc cagctgcacc aagaacgaat aagttagctg
     5521 tttcagtcac gggttcatga cactcctttc attaaaataa tcgctagtgt tatatagaaa
     5581 aaaacgacga ggttaggacc tactgaattt atgcgatagg catggtgcgt tcatatctca
     5641 actatttaat aaagttcaaa gatattacta tatatgcatc atgaattatg tcctatgcgc
     5701 tttgtcccga cctcttttat ctgttaatta tccagcaaat gtcatgaatg caattactac
     5761 accgatgata ggtaatgcct caactaaacc tacaccaatg aacatgatac ccattaattg
     5821 accacgtgct tctggttgac gtgctacacc ttcaactgtt cttgaaacga ttaaaccgtt
     5881 accgatacct gctcctaatg ctgataaacc aattgcgatt gctgctgcga ttaaattcat
     5941 aatttaattt cctcctgtga ttgtatataa ttattgaaat ttttagtgtt catctgccac
     6001 tttatgtgac atataaacca tcgaaagcat aataaagata tatgcttgga ttgttcctac
     6061 aaatattgaa aatgcttgcc aaacaattaa tcctgggata ctaataatcc aaccccatgc
     6121 tggttcgtta aagaataagc cagcaagtaa tgttaatagt atctcacctg caaatatgtt
     6181 accgtacaaa cgcagaccaa gcgttaatgt tgaagtgaac tcttcaaaaa cattaataat
     6241 tgccaatggc caaaacggct gtacataacc tttaagatat tgtttcgtac cacgcatttt
     6301 aattccataa aagtgagtta acagtattat cgttgtagac aacgttaaag tcactgttgc
     6361 atcagctgtc ggtgatttcc accacaatgt gtgatctttc gttactatag aaaacggaag
     6421 acctaacata ttagctataa aaatgtacag aatcagcgtt actgctaaga agtggaattg
     6481 accacctttt ttccaagcca tgttaccttc aatgattccc ctcacgaaat caaaaatcca
     6541 ttcaacgaaa ttttgtttgc cagttggtct ttttttcaaa ttacgcgtac aaatgatagc
     6601 aagtagaaaa acaagaaacg ccgtaacaag tatcatcaat atacttgata aattgaaaac
     6661 gatatcaaaa ccgaataaat tccaactcac gagcggggat ttgtgatcca taatctcacc
     6721 tctttcttaa tttatttgct cacctttagt aaaggacgta tgataactac aacatatgaa
     6781 atcattaaac caataattat accgatgata cttacatgcg ctttattaag gtaccaaatc
     6841 atacaggcaa taattgcaac taagtatcgc cacatatttc cagttgaaat atgcatagtg
     6901 tctggtcttt tagctttagc taaatagcat tcaaaaatac atgtgttgat tgcagaacca
     6961 gtaacaccaa tgattaatcc taagataaat gcatggtgtg tataaaggta taatcctccc
     7021 aatattatta ttagataaaa ataatattga atgaactgtt taaaaatgtt gtagaaacga
     7081 ctcattttat atgcctccgt gtgactgata tcaatcatga aaaccgttcc actcatacaa
     7141 actaaatgat aatataggcg atttgtggtg tcaattctat tcaaattgtt gtaaaaaaat
     7201 aagcatagtt agacacaatt tagacacaat tagacaaaaa gtctattttc ctcagaaatc
     7261 gcttggtttt tcagtgatta aattcaaata atacttaata tgattaacta ttctttctga
     7321 agcaaatcca tcaccgtaag gattagatgc ttcagacatt tgatgatata atctctcatc
     7381 atcgattaat tcttttgcag cttgatacac attttgctta tttgttccaa tgacttttaa
     7441 tgtgccagct tcaacacctt caggacgttc tgtaacactt cgcaaaacta aaactggctt
     7501 attaaatgat ggcgcttctt cctgaattcc acctgaatct gtcaaaataa aataagattt
     7561 tttagcaaaa ttatggaaat ctactacgtc caaaggttca atcaattcaa ttctgtcatg
     7621 actacctaaa atcttttgag ccacctctcg aactttcggg tttttatgca ttggatatac
     7681 cagtgctaaa tcagtatact catctattaa gcgtctaacc gctttaaata tattttccat
     7741 gggtttcccg atattttctc gtcggtgtgc tgtcataaga atgaattttt tgtcatggta
     7801 tttatccatg atgttagatt tataattgtc atcaactgta tatttcatag catcaatcgc
     7861 agtattacca gtgacaacaa cactttctga atatttccct tcacttaaca aatgcgatgc
     7921 agcattttta gtaggtgcaa aatgtaagtc agctaataca ccaactaatt gtctattcac
     7981 ctcttctgga aaaggtgaat atttatcata acttctaagc cctgcttcaa cgtgtccaat
     8041 cggcacttgg ttataaaatg ccgctaaacc acctgcaaat gtcgtcatcg tatcaccatg
     8101 tacaagtacc atgtctggtt tttctaattg aatcacttgt tctaattgag tgattgattt
     8161 agaagttatc tcagaaagtg tctgtcctga tttcataata ttcaaatcgt attttggttt
     8221 gatttcaaag gtacttaata ctgaatcaag catttctcta tgctgtgctg taacaacaac
     8281 aattggctcg agcatttttt cttgttccaa agctttaact aaaggagcca tctttatggc
     8341 ttcgggtctt gttccaaata tggtcataat ctttttcatc aaactactta tctccgattc
     8401 ttctatttag taccaaacaa tctatctcca gcgtcgccta accctggtgt gatatatgct
     8461 ttgtcattta gcttttcatc aagtgcagca atataaatat ctacatctgg atgtgcttca
     8521 tgcatctttt ctacgccttc tggtgctgca attaaacaca tgaagcgaat atttttagcg
     8581 ccacgtttct tcaatgaagt aatagcttca attgctgatg cgcctgttgc taacatagga
     8641 tcaacaacaa tgatttgtct ttcagtaata tcttgaggta acttagcaaa atactctaca
     8701 gcctttaatg tttcgggatc tcgatataaa ccgatatgtc caactctggc tgcaggtact
     8761 aaacttaaaa taccatcagt catacctaaa ccagctctta aaattggaac gatagctaat
     8821 tttttaccag ctaatcgttt agccgtcatt ttagttacag gcgtttcaat atcaacatcc
     8881 tgaagctcta agtctctagt tacttcatat gccatcaaca taccaacttc gtctacaagt
     8941 tctctaaatt ctttagtacc tgtatttaca tctctaatat agcttagttt gtgttgaatt
     9001 aatggatgat cgaaaacgtg tactttactc ataaaaatta ctcctatctt tgtgtatgtt
     9061 tattgatata gaggatattc agctgttaat ttcgcaacgc gttctttagc ttgttgtaat
     9121 ttttcttcat ctttactatt tttcaatgct aaactgatga tttttgcaac ttcctcaaaa
     9181 gctttttcat caaatccacg cgttgttgca gcaggtgtac ctaaacgtat accactcgtt
     9241 acaaaaggtt tttcttgatc gaacggaatg gtatttttgt tacatgtgat accaactgaa
     9301 tctaaagtct cttcagcttc tttaccagta agtcctatag acccttttac atcaacagct
     9361 actaagtgat tatctgtacc gccagaaaca attctaaatc cttcattaat taatgcttct
     9421 gcaagaactt ttgcgttttt aaccacttgt tgttgatacg ttttgaaatt attttctaac
     9481 gcttctccaa aagcaactgc ttttgctgca ataacatgct caagaggtcc accttgaata
     9541 ccagggaaaa ttgttttatc tatgtctttt ttatattctt ccttacataa aatcatacca
     9601 ccacgtggtc cgcgtaatgt tttgtgtgtt gtagttgtta caaaatcagc atattctact
     9661 ggatttggat gtaaacctgc cgctactaat cctgcaatat gtgccatgtc taccattaac
     9721 ttagcgttta cttcatctgc gatttcttta aactttttga agtcaattgt tcttgaatat
     9781 gctgatgctc ctgccacaat aagcttaggc ttatgctcta acgctaattt acgaacttca
     9841 tcataattga ttcgttctgt gtctttatct actccatatt caacgaaatt gtagaattta
     9901 ccactaaaat taacaggcgc tccatgtgtc aagtgaccac catgactcaa attcatacct
     9961 aaaactgtgt cgcccattcc taatgcaact aagtaaacag ccatgttcgc ttgtgaacct
    10021 gaatgtggtt gaacattgac atgttcagct ccaaacaatg ctttagcacg atcaattgcg
    10081 atgctttcag taacatctac aaactcacag ccaccataat atcggcgtcc tggatagcct
    10141 tcagcatact tattagtcaa cactgaacct tgtgcttcca taaccgcttc cgatacaaaa
    10201 ttttccgatg cgattaactc tatgttgcta ttttgtctct gaaattctct ctcgattgct
    10261 tctgcgataa ctttatcttg cttggtgata taagacataa aatctcccct tctttcaaaa
    10321 aaacttattg gtatttagca cgttcgccac caatcttttt cggcctagat gtggcaatag
    10381 ttacaattgc ctgtcctact tgctttactg aggtccttac aggtacacat acatgcttaa
    10441 tatgcatgcc tattaacgtt tgaccaatat caattccaca aggaacagta atatgttcga
    10501 ccacgatcgg atccttcata tgctgaaaag cgtatgttgc caaactccct ccagcatgta
    10561 catctggaac gacggaaact tcttccattg ttaatggatt atactgagat ttttctattg
    10621 ttatcgctct gttgatatgt tcacatcctt gaaaagcaaa agtaacgcct gtctctttac
    10681 tcacaacatc taatgcatta aaaatagttt ctgcaacttc catcgaaccg acagtcccta
    10741 ttttttcgcc aatgacttcc gatgttgaac atccaattaa acatatatct cctttattaa
    10801 aaaaggacat atcttttaat tcgtctaata acattgtcaa atctttcata aaagcccacc
    10861 cttcctaaaa ataaaaaagg aatatagcaa agtgctacac tcctctatta taacttattt
    10921 aactgttaac atatactaat tatacagaat tcctactagc aaataatatc ttttaatttt
    10981 aaaattaaac ttacaagttc ttcataggta tgtacataca tttcttttgt tccaccgtat
    11041 ggatctataa cttctcctgc ttcttttaca tattcatgca atgtgaaaac atgattttgc
    11101 aaaccaaagt gtgcctctat taattctttg tgcgaatacg acatcgtcaa aataatatct
    11161 gctttcaaat ctgcttcagt aaattgttgc gataaggtcg tttcagctaa atgatgttct
    11221 tcaactaagt cttcaacata attcgaaaca ccttgattgt tcacagcgaa tatacctctt
    11281 gattcaaatt gatgatttgg cataacctct tttgcaatac tttccgctaa tgggctacga
    11341 catgtgttac ctgtacaaac gaataaaatc ttcatagttc acatccttta ataatgtgat
    11401 tacctgcagc ttttaacatg cgattcataa ttgcttctgt attatcattc agctcaaagc
    11461 cgtatatata cgccgctgaa atattttcat tttcatcaag tgaatgtaac acatcataaa
    11521 gattatgact tgcttgttta acatcattgt catcctgaca taattgaatg aattgcgctt
    11581 cacttggtat aaacgccacc ttattactcg gcacaataaa agctatagaa gaccaatctt
    11641 taccgtcatt tccaattttg ctctcaatat ctgtaataat tgtaagtggt gtattgggtg
    11701 agtaatgctt atacttcata cctggtgcaa ttggctgttc agtatcatta taatcagcat
    11761 gggcgatact attcggaagt atttctgtaa tcattgctgc tgttatagaa ccaggtcttg
    11821 caattttata aggaaaagat gtgcaatcta aaaccgtact ttctaatcct tcttcacttt
    11881 gttcagcttg aacaatacca tcgatacggc cattcaaatc ttgatataca tgattgaaag
    11941 ttgttggtga aggtctacca cttaaattag cacttggagc agctagaggt tcatttatga
    12001 tttgtaataa ttgtctacct acagaatggc ttggcattct aacagcaact gatgataaac
    12061 ctccagaaac ttttcgacat agatagccta gctttaacgg caatataaac gaaatagggc
    12121 ccggccagaa tgcctgcatt aacttttcta cgcgtggatc caaagtatat gtaaaatctt
    12181 ttaattgacc tttactgtgt atatgaacaa taagcggatt gtcagatgga cggcctttag
    12241 cttcatatat tttagctaca gcttcttcat ctgtcgcatt tgctgcaagt ccataaactg
    12301 tttcagttgg taaacctatt aaaccaccgt ttaaaacaat gtcttttatt tcattaattt
    12361 taggatattg ctgtaaatct tcattatatt ctctaacatc ccaaatttta gtatccaact
    12421 taatcacgcc tttcttattt atcataatat aaagcaaaaa gctatgcact taactaatca
    12481 tagcaaaggc ataacttcta attaccattt aaatgagacg attcgatcgt ggccatttat
    12541 atctttaata atgtcgattt ttttgtcagg aaatttattt aaaattattg atttaagtgc
    12601 ctcaccttga ttgtaaccaa tttcaaaaac aactgggctg cctttttcca taacgtgagg
    12661 taaatcttca atgattgatt cataaatagc atatccatgg ttatctgcaa acaatgcctg
    12721 atgtggttcg aatctcgtaa ccgttggaga catcgtaacc atatcttttt catctatata
    12781 tggtggatta gatatcaagc cgttcaactt gataccttca ttaattaagg gctttaatgc
    12841 atcccctgtt aaaaattgta tttgtgattg atgcttctca gcattattac gagccatatt
    12901 cattgcttca agtgaaatat cagtagcaat aacatttaaa tccggctttt cacatttcaa
    12961 agtaattgca agtacaccac tacccgttcc gatatctacg attgttgcat catcttctaa
    13021 ctgttgtaag aaatgcaaca ttacttcttc agtttcaggt cttggtatca aacaatttga
    13081 gtttacatca aacgttctac cataaaatga ggcaaagcca actatatact gtataggctc
    13141 tcctaataac atacgttgta atgctaagtc gaacttcata atcatcgctt tcggcatatc
    13201 atcatgcatg tggactacaa agtccgtacg cgtccattga aatacatcta acattaacca
    13261 ttcagctcgt gtttgttcaa accctttttg ttgtgttaaa tgaattgctt catctaactt
    13321 ttctttataa ttcaccatta ttaagttctt tcaatttatc tgtctgctct gataaagtca
    13381 gtgcatctat aatttcttct aaatggcctt ccataatttg ccctaatttt tgaagcgtta
    13441 gacctatacg atggtctgtt acacggcttt gtggataatt ataagttcga atacgttctg
    13501 aacgatcacc agtaccgact gctgatttac gttgtgacgc atacttttgt tgttcttctt
    13561 gaactttcat atcgtataaa cgtgctttta acactttcat tgctttttca cggttttgaa
    13621 tttgagactt ctcagaagat gttgcaatga caccagttgg taaatgggta atacgtactg
    13681 cagagtcagt tgtgtttacg tgctgaccac ctgcaccact tgaacgatac gtgtcgattt
    13741 ttaaatcttc atttctaatt tcaatttcta catcttcaac ttctggtaaa actgccactg
    13801 tagctgttga agtatgaata cgtccacctg attctgtttc aggcacacgt tgaacgcggt
    13861 gcgcaccatt ttcaaatttc aatttactat acgcgccatt accagaaact gagaaactaa
    13921 tttctttgta accaccatgg tcactttcag acgcttctac tatttcagtt ttgaatcctt
    13981 gtgattcagc atactttgaa tacatacgca ttaaatcacc agcaaaaatc gcagcctcat
    14041 caccacctgc tgctgctctt atttctacaa taacgtcttt gtcatcatta ggatctttag
    14101 gaatcaataa tattttaagc tcttcttcaa gatttggaag ttcagcttta ataccattac
    14161 tctcctcttt taacatttct acttcttctt tatcatcagt ctcacttaac atttcttcaa
    14221 tatcagctaa ttcttctttt ttagctttat agttacgata aacatctaca gttttttgta
    14281 aatcagcttg ctctttagaa tatttacgta atttatctga atcatttaca acatctgggt
    14341 cacttaacag ttcatttaac tgttcgtatc tttcttctac aatatctaat tgatcaaaca
    14401 cttataattc ctccttatta ttatcactag gtgctacgat atggtgcgcg cgacaacgtg
    14461 gctcataact ttcattggca cctactaaga taatcggatc atcgatttta gctggtttac
    14521 catttattaa tcgttgcgtt ctactagatg aagaaccaca aacagcacaa actgcttgaa
    14581 gtttcgttac ttgttcactg acagccatca atttaggcat tggttcgaac ggttcgcccc
    14641 taaaatccat atctaatcca gcaacaataa cacggtgtcc atctgctgat agtttttcta
    14701 ctatacttac aatttcatcg tcaaaaaatt gcacttcgtc tattcctata acatcaacat
    14761 tagttaagtc gtgcgtcata atttcacttg ctttagaaat attaatcgct tcaatggcat
    14821 taccattatg agagaccact ttttctttat gatatcgatc atcaatcgcc ggtttaaata
    14881 caacgacttt ttgtttagcg tatatacccc ttcttagacg tcttattagt tcttcggatt
    14941 taccgctaaa catactacct gtaatacatt ctatccaacc ggaatggtaa gtttcataca
    15001 ttgagagttc cacctttttc aaaacataat cgctttatta tatcatattt caaaaattca
    15061 taaatgtctt tttcataatt atatcgatat tgtacatgaa caattatttt ataaatttgt
    15121 cttgcatatt tatatacagc tattctaatt tgttgcttct tattcgtgcg atgtttctac
    15181 tcaatacaaa ctttaataaa gggggaaatt tatcattatg tattaacaac atggtattga
    15241 gtcacttctt tgaacacagt cgtaagtata tacccttact acgcttataa ataaaaaagc
    15301 tgtctacaat tccattatag gatgcagaca gcttaacgat tactatatta gttgtttgat
    15361 ttaagaccaa actttttgtt gaaacgttcc acacgtccat ccgcagcagc gaatttttga
    15421 cgtccagtgt agaatggatg tgaatcagat gaaatatcta aacgaataac tgggtattct
    15481 tttccatctt cccactccat catttctgaa gatgtttttg tagaaccgct taagaatttg
    15541 aagtttgtag tagtatctaa aaagattact tggtgatact caggatgaat tccttgtttc
    15601 attattttca gctcctttgc cctgaaccat ctggaacaga gttatttgtg agtttttacc
    15661 caatactgtg taattataaa ggtaattaat tcaaaacgca agcccctata taatgtttat
    15721 taaattatag gtcgacccgt tttagtactt tcttctgcag acttttgtag ctgcttgaag
    15781 aaatcttcat tattcttaga ccttttaagt ttgcgaataa atctttcagt aaagtcagtt
    15841 gagtcagtga atagatttct taattgccat aatgtgtcta attcagattt acttatcaac
    15901 aattcttctt tacgcgttga acttctgcca atatcaattg cagggaagat acgacgttca
    15961 gacaatttac gatctaaatg taactccatg ttacctgttc ctttaaattc ttcgtaaatc
    16021 atatcgtcca tacgtgaacc cgtatcaact aatgcagttg caagtattgt taaacttcca
    16081 cccgcttcaa tatttctcgc tgcaccgaag aatgcttttg gtttgtgtaa agatgcagga
    16141 tctaaaccac ctgataatgt acgaccactt ggtggaataa ctaagttata agcgcgtgct
    16201 aatctcgtta tagaatccat taaaataatg acatcttccc caatttctac taaacgcttt
    16261 gcacgttcaa gtaataattc agctacttta acatggtgtt ctggtggttc gtcaaacgtt
    16321 gaatgaacga cttcagcagc ttctactgag cgttctaaat ctgttacctc ttcaggacgc
    16381 tcgccaacta acaaaataaa tagctttgca tctggtttgt tcgtactgat tgcattcgct
    16441 atttctttta ataacgatgt tttacctgct ttaggtggcg ccactattaa accacgttga
    16501 cctaaaccaa tcggtgttac taaatccatg atgcgcgttg aataattttg tgtttctgtc
    16561 tctaatttaa tacgctcatc tggataaagt ggtgtcaaag cttggaaatg cggacgtttc
    16621 ttcacttctt ctgcgttatg gtcattgaca aagtcaactt gtaataagcc ataatatttt
    16681 tcgttatctt taggttttct aactttccca gttactttat ccccacgttt aatttcaaaa
    16741 cgacgaattt ggctagcaga tatataaata tctttttccc ctttagaata gttcactgtt
    16801 cttaaaaaac cataaccatc tggttgtata tcatctaaga taccttccat ataatagtta
    16861 ccatcttttt ccatttgtgc ttccataata gctagaacaa gttctttttt atttaattta
    16921 ctatagttcg tcaacttaag agttttagct ttttgagtga gttccttggt agtatagttc
    16981 ttgtacaatt cgtggaatga ttcatactga ggagatgtac gttctctttc aggcatatag
    17041 tttacaccca tttcattatt ttttagttag taagtaatag ataggcatga caatgcataa
    17101 ataactgtgc tagcacaatc ccattatgct aatccccaac cttttaaata catgaataac
    17161 tatagcaaat ttttatttta attacaaaat ctatttataa aaattgcaaa attaa
//
LOCUS       DALAGF010000055        17100 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.055, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000055 DALAGF010000000
VERSION     DALAGF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17100
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.055"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            167..2698
                     /gene="secA"
                     /locus_tag="O2692_001642"
     CDS             167..2698
                     /gene="secA"
                     /locus_tag="O2692_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCZ4299275.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            2740..2844
                     /locus_tag="O2692_001643"
     CDS             2740..2844
                     /locus_tag="O2692_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299276.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
     gene            3013..4123
                     /gene="prfB"
                     /locus_tag="O2692_001644"
     CDS             join(3013..3084,3086..4123)
                     /gene="prfB"
                     /locus_tag="O2692_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCZ4299277.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            4277..4384
                     /locus_tag="O2692_001645"
     CDS             4277..4384
                     /locus_tag="O2692_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299278.1"
                     /translation="MCLLKQHMQCAFSNITYGDFQGIELIIENVLNYMV"
     gene            4532..5371
                     /locus_tag="O2692_001646"
     CDS             4532..5371
                     /locus_tag="O2692_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ4299279.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKSPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVTKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            5545..6195
                     /locus_tag="O2692_001647"
     CDS             5545..6195
                     /locus_tag="O2692_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCZ4299280.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            6192..6428
                     /locus_tag="O2692_001648"
     CDS             6192..6428
                     /locus_tag="O2692_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HCZ4299281.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            6697..8682
                     /gene="uvrB"
                     /locus_tag="O2692_001649"
     CDS             6697..8682
                     /gene="uvrB"
                     /locus_tag="O2692_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCZ4299282.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            8690..11536
                     /gene="uvrA"
                     /locus_tag="O2692_001650"
     CDS             8690..11536
                     /gene="uvrA"
                     /locus_tag="O2692_001650"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCZ4299283.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     HDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            12143..13075
                     /locus_tag="O2692_001651"
     CDS             12143..13075
                     /locus_tag="O2692_001651"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCZ4299284.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            13081..13920
                     /locus_tag="O2692_001652"
     CDS             13081..13920
                     /locus_tag="O2692_001652"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCZ4299285.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGSVSRAFLEQLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGR
                     FFIEGLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTK
                     KVK"
     gene            13928..14413
                     /locus_tag="O2692_001653"
     CDS             13928..14413
                     /locus_tag="O2692_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCZ4299286.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            14421..15860
                     /locus_tag="O2692_001654"
     CDS             14421..15860
                     /locus_tag="O2692_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCZ4299287.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDMEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYHNELFVSENDVMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            15927..16862
                     /gene="trxB"
                     /locus_tag="O2692_001655"
     CDS             15927..16862
                     /gene="trxB"
                     /locus_tag="O2692_001655"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCZ4299288.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
BASE COUNT         6232 a         2327 c         3360 g         5181 t
ORIGIN      
        1 atatttatta gtgataaact tgttgacaac agaatgtgaa tgaagtatgt cataaatata
       61 tttatattga ttctacaaat gagtaaataa gtataatttt ctaactataa atgataagat
      121 atattgttgt aggccaaaca gttttttagc taaaggagcg aacgaaatgg gatttttatc
      181 aaaaattctt gatggcaata ataaagaaat taaacagtta ggtaaacttg ctgataaagt
      241 aatcgcttta gaagaaaaaa cggcaatttt aactgatgaa gaaattcgta ataaaacgaa
      301 acaattccaa acagaattag ctgacattga taatgtcaaa aagcaaaatg attatttaga
      361 taaaatttta ccagaagcat atgcacttgt tagagaaggc tctaaacgtg tattcaatat
      421 gacaccatat aaagttcaaa ttatgggtgg tattgcaatt cataaaggtg atatcgctga
      481 gatgagaaca ggtgaaggta aaacattaac agcgacaatg ccaacatact taaatgcatt
      541 agctggtaga ggtgttcacg ttattacagt caatgaatac ttatcaagtg ttcaaagtga
      601 agaaatggct gagttatata acttcttagg tttgactgtc ggattaaact taaacagtaa
      661 gacgacagaa gaaaaacgtg aagcatacgc acaagacatt acttacagta ctaataatga
      721 gctaggtttt gattacttac gagataacat ggtgaattat tctgaagata gagtaatgcg
      781 tccattacat tttgcaatca ttgatgaggt tgactcaatt ttaatcgacg aggcacgtac
      841 gccattaatt atttctggtg aagctgaaaa gtcaacgtca ctttatacac aagcaaatgt
      901 ttttgcgaaa atgttaaaac aggacgaaga ttataaatac gatgaaaaaa cgaaagctgt
      961 acatttaaca gaacaaggtg cggataaagc tgaacgtatg ttcaaagttg aaaacttata
     1021 tgatgtacaa aatgttgatg ttattagtca tatcaacaca gctttacgtg cgcacgttac
     1081 attacaacgt gacgtagact atatggttgt tgatggcgaa gtattaattg tcgatcaatt
     1141 tacaggacgt acaatgccag gccgtcgttt ctcggaaggt ttacaccaag ctattgaagc
     1201 gaaggaaggc gttcaaattc aaaatgaatc taaaactatg gcgtctatta cattccaaaa
     1261 ctatttcaga atgtacaata aacttgcggg tatgacaggt acagctaaaa ctgaagaaga
     1321 agaatttaga aatatttata acatgacagt aactcaaatt ccgacaaata aacctgtgca
     1381 acgtaacgat aagtctgatt taatttacat tagccaaaaa ggtaaatttg atgcagtagt
     1441 agaagatgtt gttgaaaaac acaaggcagg gcaaccagtg ctattaggta ctgttgcagt
     1501 tgagacttct gaatatattt caaatttact taaaaaacgt ggtatccgtc atgatgtgtt
     1561 aaatgcgaaa aatcatgaac gtgaagctga aattgttgca ggcgctggac aaaaaggtgc
     1621 cgttactatt gccactaaca tggctggtcg tggtacagat atcaaattag gtgaaggcgt
     1681 agaggaatta ggcggtttag cagtaatagg tacagagcga catgaatctc gtcgtattga
     1741 tgaccagtta cgtggtcgtt ctggacgtca aggtgataaa ggggatagtc gcttctattt
     1801 atcattacaa gatgaattaa tgattcgttt tggttctgaa cgtttacaga aaatgatgag
     1861 ccgactaggt ttagatgact ctacaccaat tgaatcaaaa atggtatcaa gagctgtaga
     1921 atcagcacaa aaacgtgtag aaggtaataa cttcgacgcg cgtaaacgta tcttagaata
     1981 cgatgaagta ttacgtaaac aacgtgaaat tatctataac gaaagaaata gtattattga
     2041 tgaagaagac agctctcaag ttgtagatgc aatgctacgt tcaacgttac aacgtagtat
     2101 caattactat attaatacag cagatgacga gcctgaatat caaccattca tcgactacat
     2161 taatgacatc ttcttacaag aaggtgacat tacagaggat gatatcaaag gtaaagatgc
     2221 tgaagatatt ttcgaagtcg tttgggctaa gattgaagca gcatatcaaa gtcaaaaaga
     2281 tatcttagaa gaacaaatga atgagtttga gcgtatgatt ttacttcgtt ctattgatag
     2341 ccattggact gatcatatcg acacaatgga tcaattacgt caaggtattc acttacgttc
     2401 ttatgcacaa caaaatccat tacgtgacta tcaaaatgaa ggtcatgaat tatttgatat
     2461 catgatgcaa aatattgaag aagatacttg taaattcatt ttaaaatctg tagtacaagt
     2521 tgaagataat attgaacgtg aaaaaacaac agagtttggt gaagcgaagc acgtttcagc
     2581 tgaagatggt aaagaaaaag tgaaaccgaa accaatcgtt aaaggcgatc aagttggtcg
     2641 taacgatgat tgtccatgtg gtagtggtaa aaaattcaaa aattgccatg gaaaataaat
     2701 gatataaaat aactccttcc aattaaacac ctatagtttg tgttatggga ggagtctttt
     2761 tattttacaa gcgttaaata ctttaaaaaa tgtgaagaag ttgttaaacg ttgttatgta
     2821 cttagtttta aaaaatcggt ttaggcatat gtcgatgata aatgtactga tttttaacaa
     2881 taaatgcata aactaattgt cagtgtgctt atatttctta acattgttat ttaacaaaat
     2941 tatgttaaaa tttagcatta taaaagatgc aaatcaatga cttgaattga aatataaata
     3001 ggagcgaatg ctatggaatt atcagaaatc aaacgaaata tagataagta taatcaagat
     3061 ttaacacaaa ttagggggtc tctttgactt agagaacaaa gaaactaata ttcaagaata
     3121 tgaagaaatg atggcagaac ctaatttttg ggataaccaa acgaaagcgc aagatattat
     3181 agataaaaat aatgcgttaa aagcaatagt taatggttat aaaacactac aagcagaagt
     3241 agatgacatg gatgctactt gggatttatt acaagaagaa tttgatgaag aaatgaaaga
     3301 agacttagag caagaggtca ttaattttaa ggctaaagtg gatgaatacg aattgcaatt
     3361 attattagat gggcctcacg atgccaataa cgcaattcta gagttacatc ctggtgcagg
     3421 tggcacggag tctcaagatt gggctaatat gctatttaga atgtatcaac gttattgtga
     3481 gaagaaaggc tttaaagttg aaactgttga ttatctacct ggggatgaag cggggattaa
     3541 aagtgtaaca ttgctcatca aagggcataa tgcttatggt tatttaaaag ctgaaaaagg
     3601 tgtacaccga ctagtacgaa tttctccatt tgattcatca ggacgtcgtc atacatcatt
     3661 tgcatcatgc gacgttattc cagattttaa taatgatgaa atagagattg aaatcaatcc
     3721 ggatgatatt acagttgata cattcagagc ttctggtgca ggtggtcagc atattaacaa
     3781 aactgaatcg gcaatacgaa ttacccacca cccctcaggt atagttgtta ataaccaaaa
     3841 tgaacgttct caaattaaaa accgtgaagc agctatgaaa atgttaaagt ctaaattata
     3901 tcaattaaaa ttggaagagc aggcacgtga aatggctgaa attcgtggcg aacaaaaaga
     3961 aatcggctgg ggaagccaaa ttagatcata tgttttccat ccatactcaa tggtgaaaga
     4021 tcatcgtacg aacgaagaaa caggtaaggt tgatgcagtg atggatggag acattggacc
     4081 atttatcgaa tcatatttaa gacagacaat gtcgcacgat taatatatat tttaaaaccg
     4141 aggctctaaa agggcgtcgg tttttggttt ttttaaaggt agctaaataa attgtaaatt
     4201 agattttgga atatgatttg tttatgaata tttaagtaca attcggtaga tagagttaga
     4261 atatattttt taaaagttgt gtttgttaaa acaacatatg cagtgtgcat ttagtaatat
     4321 tacctatggc gattttcaag gtattgaatt aattattgaa aacgttctca attacatggt
     4381 atgaatacat tttacactat gataaaaggt tgtattcttt ttatattgtt aaccatttga
     4441 ttacatcgtt ataacaatag cttttgacaa aatgtattgt gctatagtat ttgcatactt
     4501 aaaatactaa cagcaaagga atgacagcaa gatgaaaaaa tctcttacag tgacggtttc
     4561 gtcagtgtta gcttttttag ctttaaataa tgcagcacat gcacaacaac atggcacaca
     4621 agtaaaaaca cctgttcaac ataattatgt atcaaatgtt caagcacaaa cgcaatcacc
     4681 gacaacttat acagtagttg ctggcgattc attatataag attgctttag agcatcactt
     4741 aacgttgaat caattatatt catacaatcc tggtgtaaca cctttaattt ttcctggtga
     4801 cgtgatttca cttgtgcctc aaaataaagt gaaacaaact aaagcggtta aatcaccagt
     4861 aagaaaagca agccaagcta aaaaggtagt aaaacaacct gtacaacaag catctaaaaa
     4921 agtagtagtt aagcaagcac ctaagcaagc agtaactaag acagttaatg tagcatacaa
     4981 acctgctcaa gtacaaaaat cagtaccaac tgtacctgtt gcacataact acaataaatc
     5041 agttgctaac agaggaaact tatatgctta tggaaactgc acatattatg ctttcgatcg
     5101 tcgtgcacaa ttaggtagaa gtataggaag tttatggggc aatgcaaata actggaatta
     5161 cgcagcaaaa gttgcaggat ttaaagtaga taaaacacca gaagttggcg ctattttcca
     5221 aacagctgct ggtccatatg gacatgttgg tgttgttgaa tctgtaaacc ctaatggaac
     5281 aattactgtt tctgaaatga actatgctgg atttaatgtt aaatcttcaa gaacaatttt
     5341 aaatccagga aaatataatt acatccacta agtaatatat caagacaaga ctatcctctt
     5401 agcctgttta agtaacaggt tgagaggatt ttttggtatc atttaatcag agttatataa
     5461 agaagatatt taaatgatat ttaattgatt gatatgtaaa aagaaagtat aatttatgat
     5521 taattaatgg aggaggtaat tgaaatgggt gtacatcaat attttaaaag attatcagat
     5581 atggaaagac ttataagatt acctggaaaa tttaaatatt ttgaacacaa tgttgcagca
     5641 cactccttta aagtaactaa aattgcacaa tatctagcaa cagttgaaga atatcatgga
     5701 cgaaagatta attggaaaag cttatatgaa aaagcattaa atcatgattt cgccgaagtg
     5761 tttactggtg atataaaaac acctgttaaa tatgcgagta gtgaattaaa aaaattattt
     5821 tcgcaagttg aagaagaaat ggtagagacc tttattgaag aagaaattcc attacaatat
     5881 agagatgttt ataagcaacg actgcaagaa ggtaaagatg attcattaga aggccaaata
     5941 ctttcagttg ctgataaaat tgatttgctt tatgaaacat ttggagaaat acaaaaacgt
     6001 aatcccgaag aattattttt cgaaatttat gaaatgagtc tagaaacaat tattcaattt
     6061 gaccatttag catctgtaca agattttatt aataatatca ttccagaaat gttgactgaa
     6121 aactttatac ctagaacaga attaagagaa acaaccatga acattttaaa taaaagaaaa
     6181 gaggaaaatg aatgatatgg tattttagcg cagcattctt tccatgtgtc ttggtagtat
     6241 tatttagtgt aataacaaga agtaaatggg tcggtactat tctgacatta attttaattg
     6301 gtgcctcaat ctataaagag tatttccata acgagtggat tatttttatt gatgtagtgt
     6361 cattattagc tggttattta attatagatc aactcgaatt tcataaacat caagatgaag
     6421 atcgctaaga ttaactttaa aataatgttt caaacaaatt tgttgaaaca aaatgatgat
     6481 taatataatg tgtatttaca tactaaaaat aacaagataa acgatttgat ttaaggcaag
     6541 catagttagc acaactatgt ttgtttttct ttgttcgaca tttttgcgaa caaacgtttg
     6601 ctttttgtgt gactactttg ctaaaatatg taatgagaaa gcaaagagtt gagcggaaat
     6661 ataaataata cgttgaaaga ggaggcatat gtgacaatgg ttgaacatta tccttttaaa
     6721 atacattctg attttgagcc tcaaggtgac caaccgcaag caattaaaga aatcgtggaa
     6781 ggtattaaag cggggaaaag acatcaaact ttattaggtg ctactggcac agggaaaaca
     6841 tttacgatga gtaatgttat taaagaagtt gggaaaccaa cgttaattat cgcgcataac
     6901 aaaacattag caggacaatt atatagtgag tttaaagaat tttttcctga aaacagggtg
     6961 gaatactttg taagttacta tgattattat caaccagagg catacgtacc gtctactgac
     7021 acttttattg aaaaagatgc ctcaatcaat gatgaaattg atcaactacg acattctgct
     7081 acaagtgcat tatttgaacg cgatgatgta attattattg ctagtgtaag ttgtatatat
     7141 ggtttaggta atcctgaaga atataaagat ttagtagtaa gtgttcgagt tggtatggaa
     7201 atggatagaa gtgaattact tagaaaactt gtagatgtgc aatatacacg aaatgacatc
     7261 gatttccaac gaggaacgtt tcgagtgcgt ggtgatgtag tggaaatatt cccagcctct
     7321 aaagaagaac tttgtataag ggttgagttt ttcggcgatg agattgaccg tatccgagaa
     7381 gttaactacc taacaggtga agtgttgaaa gaaagagaac attttgcgat attcccagct
     7441 tctcacttcg taacacgtga agaaaagttg aaagttgcga ttgaacgtat tgaaaaagaa
     7501 ttggaagaac gattgaaaga attacgagat gagaataaat tactagaagc gcaaaggtta
     7561 gaacagcgta ccaactatga tttagaaatg atgcgagaga tgggattctg ttcaggaatt
     7621 gaaaactatt ccgtacattt aactttgcga ccactgggtt cgacaccata tactttattg
     7681 gattactttg gcgatgattg gttagtaatg attgatgaat cacatgtgac attaccgcaa
     7741 gttcgaggca tgtataacgg agacagagcg cgtaaacaag ttttggtgga tcatgggttt
     7801 agattaccga gtgcattaga taaccgtcca cttaaatttg aagaatttga agaaaagaca
     7861 aaacaacttg tgtatgtatc tgcaacgcct ggaccatacg aaattgaaca tacggataag
     7921 atggttgaac aaattattcg tcctactggt ttactggatc ctaagattga ggttagacct
     7981 actgaaaatc aaattgacga tttattaagt gaaattcaaa caagagttga gcgtaatgaa
     8041 cgcgtacttg ttacaacgct cactaaaaag atgagtgaag atttaaccac atacatgaaa
     8101 gaagcgggta ttaaagttaa ttatctgcat tcagaaatca agacattaga acgaattgaa
     8161 ataattagag acttacgaat gggtacatat gatgttatcg taggtattaa tttattaaga
     8221 gagggtattg atataccaga agtttctcta gttgtcatat tagatgcaga taaagaaggg
     8281 tttttacgtt ctaaccgctc attaattcaa acaataggta gagctgcgcg taacgataaa
     8341 ggtgaagtca ttatgtatgc cgataaaatg actgattcga tgaagtatgc aattgatgag
     8401 acacaacgtc gtcgagaaat acagatgaaa cataatgaaa aacatggtat tacacctaaa
     8461 acaattaata aaaaaataca tgatttaatt agtgctactg ttgaaaatga cgaaaataat
     8521 gacaaagcac aaactgtgat acctaagaag atgacgaaaa aagaacgtca aaagacaatc
     8581 gacaatatag aaaaagaaat gaaacaagca gcgaaagatt tagatttcga gaaagctaca
     8641 gaattaagag atatgttatt tgaattaaaa gcagaagggt gacaagtaaa tgaaagaacc
     8701 atccatagta gtaaaaggtg ctcgtgcgca taacttgaaa gatattgata tcgaactacc
     8761 taaaaataaa ttaattgtta tgacaggttt atctgggtca ggtaaatcgt cattagcatt
     8821 cgatactata tatgctgaag gacaacgacg ttatgttgaa tcattaagtg cctatgcgcg
     8881 tcaattttta ggccaaatgg acaaaccaga tgttgataca attgaaggat tatcgccagc
     8941 aatttcaata gatcaaaaaa caacaagtaa aaatccaaga tcaactgtag caacagtaac
     9001 agaaatatat gattatatac gtttgttata tgcacgtgtt ggtaaacctt actgtccaaa
     9061 tcacaatata gaaattgaat cgcaaacagt acaacaaatg gttgaccgca ttatggaatt
     9121 agaggcacgt acaaagattc aattattagc acctgtcatc gctcatcgta aaggtagtca
     9181 tgaaaagcta atcgaagata ttggtaaaaa aggttatgta cgtttaagaa tcgatggcga
     9241 aattgttgat gtaaatgatg tacctacttt agataagaac aagaatcata caatagaagt
     9301 tgttgtagac cgattagttg ttaaagatgg aattgaaaca cgactagctg actctataga
     9361 aactgcctta gagctttcag aaggacaatt aacagtcgat gtcattgacg gggaagacct
     9421 taagttttca gaaagccatg cttgtcctat atgtggattt tcaatcggag agttagaacc
     9481 aagaatgttt agctttaaca gtccttttgg tgcttgtccg acatgtgatg gcttaggcca
     9541 aaagttaaca gtcgatgtag acttggttgt tcccgacaaa gataagacgc taaacgaagg
     9601 tgcaatagaa ccttggatac cgacgagttc tgatttttat ccaacattgt taaaacgtgt
     9661 ttgtgaagtt tataaaatca atatggataa accttttaaa aagttaacag aacgtcaacg
     9721 tgatatttta ttgtatggtt ctggtgacaa agaaattgaa tttacattta cacaacgtca
     9781 aggtggtact agaaaacgaa caatggtttt cgagggtgta gttcctaata taagtagacg
     9841 attccatgaa tctccttcag aatatacacg tgaaatgatg agtaaatata tgactgaact
     9901 accttgcgaa acttgtcatg gaaagcgatt gagtcgtgaa gcgttatctg tttatgtagg
     9961 tggtttaaat attggtgaag tagtcgaata ttcaatcagt caagcgctga actattataa
    10021 aaacattgat ttgtcagaac atgatcaagc gattgcaaat caaatattga aagaaattat
    10081 ttcccgactc acttttttaa ataatgtggg acttgaatat ttaacgttaa acagagcttc
    10141 aggtacactt tcaggtggtg aagcacaacg tattcgatta gcaacgcaaa ttgggtcgcg
    10201 tttgactggt gtcttatatg tattagatga gccatcaatt ggactgcatc aaagagataa
    10261 tgatcgatta attaatacac ttaaagaaat gagagattta ggaaatactt taattgtagt
    10321 tgaacacgat gatgatacaa tgcgtgcggc tgattactta gtggatatag gtcctggtgc
    10381 tggtgaacat ggagggcaga ttgtgtctag tggtactcct caaaaggtaa tgaaagataa
    10441 aaaatcatta acaggacaat acttgagtgg taagaaacgt attgaagtac ctgaatatcg
    10501 cagaccggct tcagatcgta aaatttctat acgtggagct agaagcaaca atcttaaagg
    10561 ggttgatgtg gacataccac tatcaatcat gacggttgtt acaggtgtat caggttctgg
    10621 taaaagctca ttagtaaatg aagtattata caaatcatta gctcaaaaaa ttaataaatc
    10681 taaagtaaag ccaggattgt acgataagat tgaaggtatt gatcaacttg ataaaattat
    10741 tgatattgat caatcaccaa taggtagaac gccacgctct aatccagcaa catatactgg
    10801 tgtgtttgat gatatacgtg atgtgtttgc gcaaacaaat gaagctaaaa ttcgaggata
    10861 tcaaaaaggg cgttttagtt ttaatgtaaa aggtggacgc tgtgaagctt gtaaaggtga
    10921 cggtattatt aaaattgaaa tgcatttttt acctgatgtt tatgttcctt gtgaagtgtg
    10981 tgatggtaaa cgatataatc gtgagacact agaggttact tacaaaggta aaaatattgc
    11041 tgacatttta gaaatgactg ttgaagaagc aacacaattt tttgaaaata ttcctaagat
    11101 taagcgcaag ttacaaacac tagttgatgt tggtcttgga tacgtcacat taggtcaaca
    11161 agctacaacg ttatcaggtg gtgaggctca acgtgtgaaa cttgcatctg aacttcataa
    11221 acgttcaact ggtaaatcta tttatatcct agatgaaccg acaacagggt tacatgttga
    11281 cgatattagt agattattaa aagtattaaa ccgattagtt gaaaatggtg atactgttgt
    11341 aattattgaa cataacctag atgttatcaa aacagcagac tatattatag acttaggtcc
    11401 tgaaggtggt agtggcggtg gtactattgt tgcgactggc acacccgaag atattgctca
    11461 gacaaagtca tcatatacag gaaagtattt aaaagaagta cttgaacgag ataaacaaaa
    11521 tactgaagat aaataagatt aaaagaagtg aaggatgtta taaatttatc cttcgcttct
    11581 ttttattaat ttagtaatga atagtagaaa gaaaagatgc gtaaaaagaa ttatgttaag
    11641 atagggtcaa tctagagtag ttaaacataa atcgaactgg gagtgggaca gaaatgataa
    11701 agaatcacta atgatttatt atgtagtggt tctttgtcat tagccacagc tattgtgtac
    11761 ttaaaaatag gaatgcatga gtgcaactca tgcataagaa atactaattt ctaaagaaaa
    11821 agtatttctt tatgttgggg ccccgccaac ttgcattgtt tgtagaattt cttttcgaaa
    11881 ttctttatgt tggggccccg ccaactaatt ccaatatatc attgtagagc ttaggtcatt
    11941 gatttttggc tcggactttt atggcgatat gaaccatgta aattaagcaa gcaataaatt
    12001 aatgattgat attgacttgt aaaataataa caataatgaa caattaatat ttattttagc
    12061 ttttcaatgt agattggtgt tatatttttg atatgataag aagagatgta agagtaggga
    12121 taaatacaat tgaggtgaac ccatgttaac gacagaaaaa ctagttgaaa cattaaagtt
    12181 agatttaatc gctggtgaag aaggactatc gaagccaatt aaaaatgctg atatatcaag
    12241 accgggctta gagatggcag gttatttttc acattatgcg tcagatagaa tacaactatt
    12301 aggaacaacg gaactatcgt tttacaattt attaccagat aaggatcgcg caggtcgtat
    12361 gcgtaaacta tgcagaccag aaacgcctgc aattattgtg acacgtggat tgcagccacc
    12421 agaagaatta gttgaagctg caaaagaatt aaatacccca cttatagttg ctaaagatgc
    12481 gactacaagt ttaatgagtc gcttaacaac gtttttagag catgcacttg caaagacgac
    12541 atctttacat ggtgttttag tagatgttta cggtgttggt gtactaatta ccggtgattc
    12601 aggaataggt aaaagtgaga ctgcgttgga attagttaaa cgtgggcata gattagtagc
    12661 agatgataat gtagaaatac gtcaaattaa taaagatgaa ctaataggga aaccaccaaa
    12721 gttaatagaa catctattag aaatacgtgg actaggtatt atcaatgtta tgactttatt
    12781 tggcgcgggt tcaatattaa ctgaaaaacg aattagatta aatattaatt tggaaaactg
    12841 gaacaagcaa aagttatatg accgcgtagg tcttaatgaa gagacgctaa gtattttaga
    12901 tactgaaatc actaaaaaaa caatacctgt aagacctggt agaaatgttg cggtaattat
    12961 tgaggtcgct gcaatgaact atcgattaaa tatcatgggc attaacacgg ccgaagaatt
    13021 tagtgaaaga ttaaatgaag aaattatcaa gaacagtcat aagagtgagg agtaggttga
    13081 atgggtattg tatttaacta tatagatcct gtggcattta acttaggacc actgagtgta
    13141 cgatggtatg gaattatcat tgctgtcgga atattacttg gttactttgt tgcacaacgt
    13201 gcactagtta aagcaggatt acataaagat actttagtag atattatttt ttatagtgca
    13261 ctatttggat ttatcgcggc acgaatctat tttgtgattt tccaatggcc atattacgcg
    13321 gaaaatccaa gtgaaattat taaaatatgg catggtggaa tagcaataca tggtggttta
    13381 ataggtggct ttattgctgg tgttattgta tgtaaagtga aaaatttaaa cccatttcaa
    13441 attggtgata tcgttgcgcc aagtataatt ttagcgcaag gaattggacg ctggggtaac
    13501 tttatgaatc acgaggcaca tggtggatcg gtgtcacgcg cttttttaga acaattacat
    13561 ttgcctaatt ttataataga aaatatgtat attaacggcc aatattatca tccaacattc
    13621 ttatatgaat ccatttggga tgtcgctgga tttattatct tagttaatat tcgtaaacat
    13681 ttaaaattag gagaaacatt ctttttatat ttaacttggt attcaattgg tcgattcttt
    13741 atagaaggat tacgtacaga tagcttaatg ctcacaagta atattagagt tgcacaatta
    13801 gtatcaattc ttttaatttt aataagtata agtttaattg tatatagaag gattaagtat
    13861 aatccaccgt tgtatagcaa agttggggcg cttccatggc caacaaaaaa agtgaagtag
    13921 tgatagtttg aggaaatttt tatcaaaaac acatcatcat acaaaccctt tatggcgtgt
    13981 ataccgtctt gttaaatttt cgaaagtttt taagaatgta attatcattg aattttcgaa
    14041 atttattcca agtatggtac tgaaaagaca tatatataaa caacttttaa atattaatat
    14101 cggtaatcaa tcgtcgatag cttataaagt aatgttagat attttttacc cagaactgat
    14161 tacgattggt agtaacagtg ttattggtta caatgtaaca attttgacgc atgaagcatt
    14221 agttgatgaa tttcgttatg gaccagtgac gataggatct aacactttga ttggtgcaaa
    14281 tgctaccatt ttacccggta taacgattgg tgacaatgta aaagttgcag ctggtacggt
    14341 tgtttcaaaa gatataccgg ataatggatt tgcatatggc aaccctatgt atataaaaat
    14401 gattaggagg tgacaatttt atggcgcaaa agaataataa tgtaattcca atgacttttg
    14461 atgatgcatt ttatcgtaaa atggctaaac agaagtttaa acaaagagaa tataaacgag
    14521 ctgctgaata ctttgaaaaa gtgttagaat tgtcacctga tgatatggaa attcaaattg
    14581 attatgcaca atgtctagtg caacttggta ttgctaaaaa agcagaacat ttattttatg
    14641 acaatattat ttataatagg catctagaag atagctttta tgaattgagt cagctcaaca
    14701 ttgaagttaa cgaaccaaac aaggcattct tgtttggtat taattatgtt attgttagcg
    14761 acgaccaaga ttatagagat gaattagatc aaatgtttga tgtgaaatat caaagtgaag
    14821 aacaaattga acttgaagct caattgtttg tagttcaaat actattccaa tatctttttt
    14881 ctcaaggtcg attaaaagat gcaaagaatt atgtcttaca tcaaccacaa gaagttcaag
    14941 atcatcgtgt agtacgtaat ttattggcaa tgtgttattt atatctcggt gaatatgata
    15001 cggctaaagc attgtacgaa gcactattac aagaggatag tacagatata tatgcattat
    15061 gccattatac tttgctactt tataacacta aggaaaatga acaatatcaa aaatatttaa
    15121 aaatattaaa caaagttgta cctatgaatg acgatgaaag ttttaaatta ggtattgtat
    15181 taagttattt aaagcagtat cgtgcatcac aacaattgtt gtacccttta tataaaaaag
    15241 ggaaattttt atcaattcaa atgtacaatg ctttagcata taattattat tatttaggtg
    15301 aagaagacga aagtcattac tactgggata aattgaagca aatttctaaa gtggaaattg
    15361 gacatgcgcc ttgggtaatt gaaaatagca aagaagtttt tgaccaacat attttgccat
    15421 tacttcaaag tgatgacagt cattatcgtt tatatggtat ttttttattg gatcaattaa
    15481 atggtaaaga aattgtgatg acggaaagta tttggcaggt tttggaaaat ctaaataatt
    15541 atgagaaatt gtatttaacg tatttagttc aaggtttaac gctcaataaa ttagacttca
    15601 ttcatcgcgg cttattaacg ctttaccata atgaattatt tgtaagtgaa aatgatgtaa
    15661 tggttgcatg gattaatcaa ggtgaactca taattgctga aaaagtagat ttaactgatg
    15721 ttgagccata tatcggtgcg tttatttatt tgtattttaa aaatcaacct cgaaacgtta
    15781 caaagaagca aattacaaca tggttaggca taacacaata taaactgaac aaaatgattg
    15841 aatttctctt gagcatatag atttatgaaa agttagattt attatataat gcgcataatg
    15901 attaataatg aggaggcgtt aataaaatga ctgaaataga ttttgatata gcaattatcg
    15961 gtgcaggtcc agctggtatg actgctgcag tatacgcatc acgtgctaat ttaaaaacag
    16021 ttatgattga aagaggtatt ccaggcggtc aaatggctaa tacagaagaa gtagagaact
    16081 tccctggttt cgaaatgatt acaggtccag atttatctac aaaaatgttt gaacacgcta
    16141 aaaagtttgg tgcagtttat caatatggag atattaaatc tgtagaagat aaaggcgaat
    16201 ataaagtgat taactttggt aataaagaat taacagcgaa agcggttatt attgctacag
    16261 gtgcagaata caagaaaatt ggtgttccgg gtgaacaaga acttggtgga cgcggtgtaa
    16321 gttattgtgc agtatgtgat ggtgcattct ttaaaaataa acgcctattc gttatcggtg
    16381 gtggtgattc agcagtagaa gagggaacat tcttaactaa atttgctgac aaagtaacaa
    16441 tcgttcaccg tcgtgatgag ttacgtgcac agcgtatttt acaagataga gcattcaaaa
    16501 atgataaaat cgactttatt tggagtcata ctttgaaatc aattaatgaa aaagacggca
    16561 aagtgggttc tgtgacatta acgtctacaa aagatggttc agaagaaaca cacgaggctg
    16621 atggtgtatt catctatatt ggtatgaaac cattaacagc gccatttaaa gacttaggta
    16681 ttacaaatga tgttggttat attgtaacaa aagatgatat gacaacatca gtaccaggta
    16741 tttttgcagc aggagatgtt cgcgacaaag gtttacgcca aattgtcact gctactggcg
    16801 atggtagtat tgcagcgcaa agtgcagcgg aatatattga acatttaaac gatcaagctt
    16861 aattcgaagt cgaattaaga tgttgagctg taaattattt ggatatttat tttaatagtg
    16921 tcatcacagc gttaaaataa tgtcttactt ttaaattaaa gcaaattata tagaaaacta
    16981 gaacttagta cgtatcattt gtgcgtttca atgagttcta gtttttttat atgttatatt
    17041 aaacttataa ctttatggga gtgggacaga aatgataaag agccactaat gatttattat
//
LOCUS       DALAGF010000056        17019 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.056, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000056 DALAGF010000000
VERSION     DALAGF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17019
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.056"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(107..862)
                     /locus_tag="O2692_001656"
     CDS             complement(107..862)
                     /locus_tag="O2692_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCZ4299289.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWIISILIVFAIVQLIL"
     gene            complement(862..1122)
                     /gene="cstR"
                     /locus_tag="O2692_001657"
     CDS             complement(862..1122)
                     /gene="cstR"
                     /locus_tag="O2692_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HCZ4299290.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKAAADDEESSQELINEAVNLLVKSK"
     gene            1259..2326
                     /gene="cstA"
                     /locus_tag="O2692_001658"
     CDS             1259..2326
                     /gene="cstA"
                     /locus_tag="O2692_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HCZ4299291.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEEENDS
                     YDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
                     IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAA
                     LIIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPKKNIRMPLSK
                     MNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEV
                     EYGGVGTYIGATENANHNLFI"
     gene            2357..3691
                     /gene="cstB"
                     /locus_tag="O2692_001659"
     CDS             2357..3691
                     /gene="cstB"
                     /locus_tag="O2692_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HCZ4299292.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            3710..4903
                     /gene="sqr"
                     /locus_tag="O2692_001660"
                     /pseudo
     CDS             3710..4903
                     /gene="sqr"
                     /locus_tag="O2692_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
     gene            complement(5570..6556)
                     /locus_tag="O2692_001661"
     CDS             complement(5570..6556)
                     /locus_tag="O2692_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCZ4299293.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
     gene            6865..7068
                     /locus_tag="O2692_001662"
     CDS             6865..7068
                     /locus_tag="O2692_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299294.1"
                     /translation="MEGLKHSLKSLGWWDLFFAIPIFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            7117..7413
                     /locus_tag="O2692_001663"
     CDS             7117..7413
                     /locus_tag="O2692_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HCZ4299295.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKRRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSAQQKLQDNTLVSPYPGSKEMILILDFTEATNLTDLFKTIKNDLKK"
     gene            complement(7485..8705)
                     /locus_tag="O2692_001664"
     CDS             complement(7485..8705)
                     /locus_tag="O2692_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4299296.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNM
                     MIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAI
                     LSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMN
                     TVSDKYVGRSFTIWTSISLLLQSLIAPFLGRWINEINDKFGFYIILILSLLIFVTLLL
                     VNKTGKIKYAHKEE"
     gene            complement(8698..9248)
                     /locus_tag="O2692_001665"
                     /pseudo
     CDS             complement(8698..9248)
                     /locus_tag="O2692_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
     gene            9361..10221
                     /locus_tag="O2692_001666"
     CDS             9361..10221
                     /locus_tag="O2692_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ4299297.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLERQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKEMKDFKLNDIQNHTLLVTEKGCSYREQLEMILYKYSFSTKQMIEFL
                     GIESLKKHLKNSGGIALLPEFIVADELENKSLFQIDVDVDIPNLETTLIINPESNKQV
                     LESFVKDVFL"
     gene            complement(10576..11109)
                     /locus_tag="O2692_001667"
     CDS             complement(10576..11109)
                     /locus_tag="O2692_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HCZ4299298.1"
                     /translation="MHAMQYHVKLPSDYNMEIIRDRVRLNGYKTDGFKNLIIKAYLIS
                     QTTTNCITNTYSPLYLWRSSEGMTEFIFNGFYDNVISSFGWQNINIGVIYSMNITDSV
                     KHSLYALEEYIDIFPTLSLKEIEIKKLFRIFDNAVAEIIIYNPDKWKFVKYSFIENLP
                     KSLNPNIKIYDVLYISQ"
     gene            11200..11943
                     /locus_tag="O2692_001668"
     CDS             11200..11943
                     /locus_tag="O2692_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCZ4299299.1"
                     /translation="MIKLNFNDLEIFITVCEEASINKAAIKLRYAQSNISQRISKLEN
                     ELGVVLLFRNQKGAKATKAGEEFLAYSKKVLRDTETIKNKMKNNTMSILCSELLFNYL
                     SESEEIMMSNNSINFISSGNIRKAIEKNNYDKVISFIKINDSNYRLSNVDTMKVTLYS
                     NGSNYDKEALLINKDEFCPLRKITLDNKLDSQRVMEIDSLAAIINLVKQGKGKALLPM
                     TFENKRDIVQDISKIFEVNYYTYNHIMHH"
     gene            12377..12979
                     /locus_tag="O2692_001669"
     CDS             12377..12979
                     /locus_tag="O2692_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299300.1"
                     /translation="MLISLVIPVFALLVIGGIIWMIIEGIVHISKKNKAIDNFFNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSAFLFWLVILTFGIAGIIFLMPYGLCF
                     LPFYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNITER"
     gene            13246..16398
                     /locus_tag="O2692_001670"
     CDS             13246..16398
                     /locus_tag="O2692_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HCZ4299301.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNAERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSEIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQHIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            16455..16937
                     /locus_tag="O2692_001671"
     CDS             16455..16937
                     /locus_tag="O2692_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299302.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKR
                     "
BASE COUNT         6285 a         2344 c         2859 g         5531 t
ORIGIN      
        1 tttcatcaaa aatttttatt tatataaatc cgaactgcat acatatttgt ttaaataaga
       61 ggtattattt ttcgggaaat tgctgtctga gttaaaagga ttagttttat aaaatgagtt
      121 gaactatagc aaaaacgatt aaaatactga taatccattt ttgtattatg ttagggactt
      181 ttttacttaa ttttaaccct attggagcaa atataatact ccctattata aggaataagg
      241 cgtcatataa agggatataa ccttgaataa gtttgatgac aaaagcacca attgaagata
      301 taaaagcaat tactatacta ttagcgacta cagtattcat tggtaatttg aataaaacca
      361 ataatatagg aataataatg aaggcaccac ctgcacctac tatacctgaa ataataccaa
      421 tgaaaaggcc aatgataact aataaatatt tattaaatga agacttttcg gaactaggtt
      481 tcactttaat aaacattaat gttaatgcaa gtaaagcaat aatgatatat accgtattta
      541 caaatgtagc atcaaataaa tttgctagaa atgcacctaa catactccct ataatcatgc
      601 cgccacccat ataaagaact aattgtggcg agaactctgt tttttttcga gcttttaatg
      661 agccacttaa tgtactgaaa aagacttggc tagaagtaag acctgatgcg atatatgcgc
      721 tatatgcagg ggctccgaat aatggtggta ataataaaat agctggataa ataatgatag
      781 caccacctac gcctactaga ccagatatga acccaccgaa taccccaatg agtaacatga
      841 taactatatt aacaatatcc attacttact tttcaccaat aagtttacag cttcattaat
      901 taactcttgg gagctttctt catcatccgc agctgctttt acacattcta ttaaattctc
      961 actaataatg atacccatca agcgttggag tgaactcttt gatgcactta tttgtgtaat
     1021 gacatcttta cagtcttttc cttcctccat cattttaata attccattta gttgcccttg
     1081 tattctatta atacgattaa tcattttttt atcataattc atagtcatac ctccactttt
     1141 aattgaataa aaatatatta atagataaac acaaatgtgt caaatacccc tagaggtatt
     1201 tgacaagttc catccaactg tttaaaatac ccctacaggt atttttaggg aggttattat
     1261 gaaacaatac ggagaaaagt ttatcgatga atttagtaaa gcagaattgg aaaaactagc
     1321 caagcaaggg caattaattg acgttagaac agaagaggag tatgcattag gacatatcaa
     1381 tggttccata cttcatcctg ttgatgagat tgagtcattc aataaagaaa aaaataaaac
     1441 ctattatgta atctgtagaa gtggtaacag aagtgctaat gctagtaaat atttagctaa
     1501 acaaggttat aacgttataa atcttgatgg tggttataaa gcttatgaag aagaaaacga
     1561 tagttatgat acacaagaag aatataaaag tatagaaatt aaagcagatc gtaaacaatt
     1621 taactatcgt ggtcttcaat gtccagggcc aattgtaaaa attagtcaag aaatgaagaa
     1681 tattgaagta ggtgaccaaa ttgaagtcaa agtcacagac cctggattcc ctagtgacat
     1741 taaaagttgg gtgaaacaaa caaggcatac tttagttaag cttgatgaaa ataacaatgg
     1801 aattaatgcg attattcaaa aagaaaaagc aaaagattta gatataaatt attctgctaa
     1861 aggtactaca attgtattat ttagtggaga attagacaaa gctgtagcag cgttgattat
     1921 tgcaaatggt gctagagctg ctggaaaaga tgtaactatc ttctttactt tttgggggct
     1981 taatgcatta aaaaaagtgc aaacagttaa tgttaaaaag caaggtattg caaaaatgtt
     2041 tgatttaatg ttgcccaaaa agaatatacg aatgcctctt tccaaaatga atatgtttgg
     2101 tttaggaaat atgatgatgc gctacgtaat gaaaaagaaa aatgttgatt cattaccaac
     2161 acttatcaat caagctattg agcaaaatat caaattaatc gcttgtacga tgagtatgga
     2221 tgtcatgggt attcagaaag aagaacttag agatgaagtt gagtacggtg gtgtaggcac
     2281 ttatattggt gctactgaaa atgcgaatca taatttattt atctaattaa atctattaat
     2341 aaaaggagtt gttatcatgt tttttaaaca gttttacgat aatcatttat ctcaagcatc
     2401 atatttagtg ggttgtcaac gtacaggaga ggcaataata atagaccctg ttcgtgattt
     2461 atcgaaatat atagaagttg cagattctga aggtttaaca attacacaag ctacagaaac
     2521 acatattcat gctgattttg cttcaggaat tcgtgatgtg gctaaacgct taaatgcaaa
     2581 tatatatgtg tctggcgaag gtgaagatgc attagggtat aaaaatatgc catcaaaaac
     2641 acaatttgtt aaacatggag atatcattca agtaggcaat gttaaattag aagttctgca
     2701 tactccagga cacacgcctg aaagtattag ctttttactc actgatttag gtggtggttc
     2761 aagtgttccg atgggattat ttagtggtga ctttattttt gttggtgata taggtagacc
     2821 tgatttatta gaaaaatctg ttcaaataaa gggttctaca gaaattagcg cgaaacaaat
     2881 gtatgagtcc gttcaaaata ttaaaaattt accagactat gttcaaatct ggccgggtca
     2941 tggtgctgga agcccttgtg gtaaagcatt aggtgccata cctatatcta caataggtta
     3001 tgagaaaatt aataactggg catttaatga aattgatgag actaaattta ttgaatcatt
     3061 aacatcaaat caaccagcac caccgcatca ttttgcacaa atgaaacaag ttaatcagtt
     3121 tggtatgaat ttatatcaat catatgatgt ttatcctagt ttagataata agagagtagc
     3181 atttgatctt cgtagcaaag aggcctttca cggtggccac acaaaaggaa caatcaatat
     3241 accatacaac aaaaacttta ttaatcaaat tggttggtac ttagattttg aaaaagatat
     3301 agatttaatt ggagataaat ctactgttga gaaagcgaaa cacactttac aattaattgg
     3361 gtttgataag gtagcaggct atcgtttgcc aaaatcaggc atttcaaccc agtccgttca
     3421 tagcgctgat atgacaggta aagaagaaca tgtattagac gtacgtaatg atgaagagtg
     3481 gaataatgga cacttagatc aagcagttaa tattccgcat ggtaaattat taaatgaaaa
     3541 tattcctttt aataaagagg ataaaatata tgtacattgt cagtcaggtg ttagaagttc
     3601 aattgcagtg ggtatattgg aaagcaaagg ttttgaaaat gtggtgaata ttagagaagg
     3661 ctatcaagat tttccagaat cattaaaata atttaaggat gtggaaaaaa tgaataagca
     3721 ttatcaaatt gttattattg gtggcggtac agcaggtgtt accgtagcat caagactatt
     3781 aagaaaaaat caaaacttaa aagagaaaat agcaattata gatccagcag accatcatta
     3841 ctatcaacca ttatggacgt tggttggtgc aggggtatct agtttgaaaa gttctcgtaa
     3901 agatatggaa agtgttatac ctgaaggtgc taactggata aaacaggctg tttcaagttt
     3961 ttaacctgaa aataatagcg ttattttagg agataataca gtcgtttatt atgatttttt
     4021 agtagtagct ccaggattac agattaattg gtcttcaatt aaaggactaa aagaaaatat
     4081 aggtaaaaat ggtgtttgct ctaactattc acctgactat gttaacgaaa cttggaacca
     4141 aatttctaat tttaaacaag gaaatgccat ttttacgcat ccaaacactc ctataaagtg
     4201 tggaggtgcg cctatgaaaa ttatgtattt agctgaagat tattttagga aacataaaat
     4261 ccgttctaac gctaatgtga tatatgcaac gccaaaagat gctttatttg acgtaggaaa
     4321 atataataaa gaattagaaa ggattgttga agaaagaaat ataacagtca attataatta
     4381 taaccttgtt gaaatcgacg gtgacaaaaa agtggctaca ttcgaacata tcaaagcata
     4441 cgatagaaaa acaataagtt atgatatgtt acatgtaaca ccacctatgg gtcccttaga
     4501 tgtagtaaaa gaaagtacac tttcagatag tgagggttgg gtagatgtta acccaaccac
     4561 attacagcat aaaagctact ctaatgtatt tgcacttggt gatgcttcaa atgtacctac
     4621 ttcaaaaaca ggcgcagcta ttcgtaagca agcacctatc gtcgctaata atttattgca
     4681 agtgatgaat aatcaaatgt taacgcatca ttatgatggt tatacttcat gccctattgt
     4741 tactggatat aataggttaa tacttgcaga gtttgattat aataaaaata ctaaagaaac
     4801 aatgccgttt aatcaggcca aagaacgtag aagtatgtat atatttaaga aagatttatt
     4861 acctaaaatg tattggtacg gcatgctaaa aggattaata taataaagta cagaaaacaa
     4921 taaattttta atgaaaaatc ttttactata aaagattaag tatttaaatg acgtgtcagt
     4981 gttgtgttta tatgtcgtga atttttagct ctaaatagta taagattgaa aaagttgtta
     5041 ctgttttaaa tgatcacgat gaagtcattc aataagaatg attatgaaaa tagaaacagc
     5101 agtaagatat tttctaattg aaaatcatct cactgctgtt ttttaaaggt ttatacctca
     5161 tcctctaaat tatttaaaaa taattaatgg tatttgagca cgtttagcga ctttatgact
     5221 gacattacca atttccattt cttgccagat attcaaacca cgtgtactca aaatgatagc
     5281 ttggtatgta cctccaatag taatttcaat aactttgtct gttgaacact aagagcaatt
     5341 ttaatttcat aatgtgttgt aaacattttt tttgattgga gtttttttct gagttaaacg
     5401 atatcctgat gtatttttaa ttttgcacca tttccaaaag gataagtgac ataagtaaaa
     5461 aggcatcatc gggagttatc ctatcaggaa aaccaagata atacctaagt agaaagtgtt
     5521 caatccgtgt taaattggga aatatcatcc ataaacttta ttactcatac tataattcaa
     5581 ttttaacgtc ttcgtccatt tgggcttcaa attcatcgag tagtgctcgt gcttctgcaa
     5641 ttgattgtgt gttcatcaat tgatgtcgaa gttcgctagc gcctcttatg ccacgcacat
     5701 agattttaaa gaatctacgc aagctcttga attgtcgtat ttcatctttt tcatatttgt
     5761 taaacaatga taaatgcaat ctcaatagat ctaatagttc cttgcttgtg tgttcgcgtg
     5821 gttctttttc aaaagcgaat ggattgtgga aaatgcctct accaatcatg acgccatcaa
     5881 tgccatattt ttctgccagt tcaagtcctg tttttctatc gggaatatca ccgttaattg
     5941 ttaacaatgt atttggtgca atttcgtcac gtaaattttt aatagcttcg attaattccc
     6001 aatgtgcatc tactttactc atttctttac gtgtacgaag atgaatagat aaattggcaa
     6061 tgtcttgttc gaagacgtgc ttcaaccaat ctttccattc atcgatttca tagtagccaa
     6121 ggcgtgtttt aacacttacc ggaagcccac ctgctttagt cgcttgaata atttcggcag
     6181 caacgtcagg tcttaagatt aagccggaac ccttaccctt tttagcaaca tttgctacag
     6241 gacatcccat atttaagtct atgcctttaa agcccatttt agctaattga atactcgttt
     6301 cacggaactg ttctggctta tctccccata tatgagcgac catcggctgt tcatcttcac
     6361 taaaagttaa gcgtccgcgc acactatgta tgccttcagg gtggcaaaag ctttcagtat
     6421 ttgtaaattc agtgaaaaac acatccggtc tagctgcttc acttacaacg tgtcgaaaga
     6481 cgatatctgt aacgtcttcc attggcgcca aaataaaaaa tggacgtggt aattcactcc
     6541 aaaaattttc tttcataata tatttatacc ctctttataa ttagtatctc gattttttat
     6601 gcatgatgat attaccacaa aagcctaact tatacaaaag gaatttcaat agatgcaacc
     6661 atttgaaagg gaagtctaag agtagtctaa aataaatgtt gtggtaagtt gatcaataca
     6721 aagatcaagg attatagtat taaattgttc attattaatg atacactact tatgaatatg
     6781 attcagaatt ttctttggct acttttacag taaagcgatt ttttagttat cttataacaa
     6841 agacaaattt ataaaggtga tattatggaa ggtttaaagc attctttaaa aagtttaggt
     6901 tggtgggatt tattttttgc gatacctatt tttctgctat tcgcatacct tccaaactat
     6961 aattttataa ctatatttct taacattgtt atcattattt tcttttccat aggtttgatt
     7021 ttaactacgc atataattat agataaaatt aagagcaaca cgaaatgaat cattaatacg
     7081 aatgtgatta aacataaaac tgaaggagcg attacaatgg cgactaagaa agatgtacat
     7141 gatttatttt taaatcatgt gaattcaaac gcggttaaga gaagaaagat gatgggagaa
     7201 tatattattt attatgatgg cgtggttata ggtggtttgt atgataatag attattggtc
     7261 aaggcgacta aaagtgccca gcagaaattg caagataata cattagtttc gccatatcca
     7321 ggttctaaag aaatgatatt aattctagac tttaccgaag caacaaatct cactgattta
     7381 tttaagacca taaaaaatga tttgaaaaag tgaagtagtg aagtgtgggt gcagagagaa
     7441 ctaagcccat cgataaatgg tcgcttgtta aagaagagtg acggtcactc ttctttatgt
     7501 gcatatttta ttttgcctgt tttgttaaca agcagcagtg taacaaatat gagtaaggat
     7561 aaaatgagta taatatagaa accgaattta tcattaattt cattaatcca tcttcctaaa
     7621 aatggagcaa ttaaactttg cagtaacaat gaaattgacg tccatatcgt aaatgagcga
     7681 ccgacatatt tatctgaaac agtgttcatt atagctgtat tcatataaat tctgattgat
     7741 gaaattgagt agcctagtat aaatgatcct atgaataagt aaaatgctga gtttatccaa
     7801 ataaatagtg ctgaatttat gactaatatg aaatataaca aaaatatcaa tactttagtt
     7861 gagattttct tcgaaagaat agctgaaatt aaacctgcac ataatcctcc aatgccatat
     7921 aacatatctg aaaaaccaaa ttgtacagac gaaagtttta aaacattata aacatatcct
     7981 ggtaatgata tgttaaagat cattgtaaac accattggta tgattgaaat aactccaaaa
     8041 ataaatatca tcatgttgtc ttttaaaaat ttccatccta ataaatattc ttgcaataag
     8101 ctatttgttg attcttcctc tgaatgagtt ggtttatcta catgcaatct aaataacata
     8161 aaaatgctga ttagaaacat cattatagtc atcgctataa ttagagtgaa tccatttatt
     8221 ttatataata ttcctgataa tccacctgca ataaacatac ctgtttgcaa actaatctct
     8281 aatagagaat ttgcatctgt atattgatct ggttttaata tctgtttaac taaacttcta
     8341 gatgttgcca tatacgtagt ccaacctatc ccattaacaa tcgcaaatcc aatcactaaa
     8401 taggtctcaa atcctatcat tacgagtgct atgacaatga gtaaatataa tattacctga
     8461 agaagatagg tgattaaaat aatatttcgt ctattatatt tatcggctag tccacctatt
     8521 ataggagaag ctaagaaacc ggataataca tttaaagcta acataatacc taatagttgg
     8581 gagtcgttag ttttgtcgat taaataccaa ttagccccaa ctgtactaat tccaactccg
     8641 aatgctgaaa ttatttctgc taaaaagaac aatctgaagt ttttaatttt caacaaatca
     8701 gtcataaata tcaccttttt tcaatgcaag atttatattt gcgagctctt ctataaattg
     8761 agagtcgttt aaaatatcga atggtttatt gccttcgccg atcaaaaatc tatagttagt
     8821 aatattcata aactcaaata ttaatttgaa ttggttgact aaaggttgtg ctttggtatg
     8881 cggattgtcg ccacctatga ttaaaatcaa atactttttt tgtgacatga tttctttgaa
     8941 gttgtctatt tgcgtatctc gcaatgactc ggtccatcta tcaatgaaga gttttaaaga
     9001 cgcactcatg gagtaccaat agagtggtgt tgaaaaaata ataatatctg attctaaaac
     9061 tttgattaaa atttgttcgt aatcatcatt atgaaagttg gcacctttgc tatgacgatt
     9121 atctgtaacc ttttcaatgt tgctttgata taaattcaca aagttgactt ttaaattctt
     9181 caatagattc tctactgcga tagctgaatt gccacctttc tactacttcc aaataaacaa
     9241 gtaatcatag tcataactcc tttcgattta cctttataat aatatttaat atattattat
     9301 taaatcagaa ttcttagaat tcaggattcg ataaaaggga accctaaagg aggtactact
     9361 ttgaacttaa acattttaaa atcattttta gtaactagtg aaacaaagaa ccttactaaa
     9421 gcatcagagt tacttaacta ttcacagtca actgtatcta cacatattga aaaattagaa
     9481 aggcaattag atgttaaatt attttataga aaaaaatatg gtatggaact aacggaagaa
     9541 ggcttagcat acgttaaata cgctaaagtg attttagata gtaatagcga atacgagaga
     9601 gaaataaaag gactttacaa taagaaggta aatataagta ttaacatgca agaaagtcag
     9661 tatttgtatc gctactataa taagattagt gaatggttag ctgaacaccc atatgtaaac
     9721 ttgaagttta aatccgcaca ttctaatttc tatattaaag aagaaattgc taatttcaaa
     9781 tcggatatta gccttatcac agacgaaaag attattaata gtaacttaac tgctattcct
     9841 ataactgaag aacgtttaat atttgttact aacaaagaga tgaaagattt caaattaaac
     9901 gatattcaaa accatacatt acttgttact gaaaaagggt gtagttacag agaacagtta
     9961 gaaatgattt tatataaata tagcttttca acaaaacaaa tgattgaatt tctaggaatt
    10021 gagtcattaa aaaaacactt gaaaaattca ggtgggattg cgctattgcc ggaatttatt
    10081 gttgcagatg aattagaaaa taaaagtttg tttcaaatag acgtagatgt cgatattccg
    10141 aatttagaaa caacattgat aattaatcct gaatcgaata agcaagtact tgaatctttt
    10201 gtaaaagatg tttttttata attattggtg aaaacgtgta gttatggtga aactcaaaga
    10261 taataattta aatgagatgt taatgaaaaa gtaattcaat ataaaaacag gtgatttata
    10321 tctttaataa ggattatttc tagttgaaat ttcaaattgc gggcattcat tttcaaaata
    10381 gtaaagaatt taatagatga aaacctttga atcatactag ttagtatgga ttgattaata
    10441 ttttaacctg ctaaatgagt agcttatgta ttttaaatag ctgtcatcta gcaggttctt
    10501 tattattaat aagttcagat aaattatttt agtctgtttt ctaatgttga taataataac
    10561 tttaaatctg tactttcatt gtgagatata tagtacatca taaattttta tatttggatt
    10621 gagactcttt ggaagatttt caataaatga atatttcaca aacttccact tatcaggatt
    10681 ataaattata atttctgcaa ctgcattatc gaagattcta aacaattttt tgatttctat
    10741 ttcttttaga gataaagtag ggaaaatgtc gatatattct tcaagcgcat aaagactatg
    10801 tttaacagaa tcggttatgt tcattgaata tatcacacca atatttatat tttgccatcc
    10861 aaaagaagaa ataacattat cataaaatcc attgaatata aattcagtca tcccctctga
    10921 acttctccat aaatataatg gtgaataagt atttgtaatg caattagtag ttgtttgact
    10981 tattaaataa gcttttataa ttaaattttt aaatccatca gtcttataac catttaatcg
    11041 aactctatct ctaattattt ccatattgta atcactaggc aacttaacat gatattgcat
    11101 tgcatgcatt tagcataccc ccttttataa aaaggatagc aataataagt aaaatctcat
    11161 attatccaat tgtgatatag ttatcataaa aagtgatagg tgattaaatt gaactttaat
    11221 gatttggaaa tttttataac tgtatgtgaa gaagcatcta tcaataaagc tgcaattaaa
    11281 cttagatatg cacaatctaa tatatctcaa agaattagca agcttgaaaa tgaattaggt
    11341 gtagttttgc tttttagaaa tcaaaaaggt gctaaggcaa ctaaagcagg cgaagaattc
    11401 ttagcgtata gcaaaaaagt attaagagat acagagacta taaaaaataa aatgaaaaat
    11461 aatactatgt ctattttatg ctcagaactg ttatttaatt atttatctga gagcgaagaa
    11521 attatgatgt cgaataactc aattaatttt atttctagtg gaaatattag aaaagctata
    11581 gaaaaaaata attatgataa ggttatttca ttcataaaaa ttaacgactc aaattataga
    11641 cttagtaatg ttgatactat gaaagtaacg ctttacagta atggaagtaa ttatgataaa
    11701 gaggctttac taataaataa agatgagttt tgtcctttaa ggaaaataac tttagataat
    11761 aagcttgatt cacaacgggt aatggaaatt gattcattgg cagcaataat aaacttagtt
    11821 aaacaaggaa aaggaaaggc tttattacct atgacttttg agaataaaag agatatagta
    11881 caggacattt ctaagatatt tgaagttaac tactatactt ataatcatat tatgcatcat
    11941 taattgaaag ttgattgagt ttaaattaca ggaacgattt taaaagtcta gtagttagaa
    12001 tgagcgatgt gacagaaaag aaattatgcc ttttattttg taaattcgtt gttttaaagt
    12061 aaaggtcctt gtagtatagg aaaaagtgga tgacttctcc gcctttacca ccctttcaag
    12121 cataatgata actgctaaat attgattata gcaccattgc aaaactaatc attaattgtc
    12181 atattcaact attttgaaaa gtatcaattt cacattatca aattattgag tatctcaatt
    12241 tattgttata cacaaacagt atttttgtta tcatagagtt aataaatata ataaattaaa
    12301 caagtaactt tacaaaaatt agaatgattc aattcaatca taattgtttg aaaatagagg
    12361 tgagcaggtg aacgatatgt taattagtct tgtaatccca gtttttgctt tgttagttat
    12421 tggtggtatt atttggatga ttatagaagg tatagtacat atttcaaaaa agaataaagc
    12481 aattgataac ttttttaacc aagttaataa agtaagtgag acatataaat tcgctactac
    12541 ttttttattt ctaatcttgg ctacggctgg tatttctcaa ttttatctat attatatagt
    12601 atcagcgttt cttttttggc ttgtcatact tacctttggc attgcaggta tcattttttt
    12661 aatgccatat ggattatgtt ttctaccgtt ttataagcaa aaaaagaaaa aacagacatt
    12721 taaaaaatac atggtttaca ctacgattgg tttgtcaatt tgtctaggct tatctctagt
    12781 tttggttcac actacgaaaa tttatatgga cgaaggtggc gtaagatact attacggtag
    12841 ttttgtaatg aaacaagcgg gcggttatgc ttatttagct ttagcggtac tttcaacgtt
    12901 gttaattgtt gcgaaaaaag ctacaaataa aaataaagaa atcgaaaccg tcgacaatac
    12961 aaatataacg gaaagataat taagggagtg ctcattcagg agtgctcttt tttgatgtcc
    13021 aaatttagtt gcaaatgaag gcatagaaga aatttttata gtcatttatt ggataatgaa
    13081 ttatggtcat atcgattaat tttattagtg aggattttac aaacatttgt tgaataaata
    13141 tggtaatgat aatatatgaa tatttttgaa aaaacactaa tatgattaaa ttatttgtta
    13201 aaatagcatt aagtaaaaaa caaataggga acaaggtggg atttcatgag tcaattgcta
    13261 aatgatacgt tatcggcttg gttgttaatt gaatctttaa gtccaggaga agtaaatttt
    13321 acagcggaag atatactctc agctgaacat tttaaaaatg gtgcaaagca agcacaactt
    13381 caaagttttg atgaatattt tgaaatatgg aatgctgaac gctttattat atcagaagaa
    13441 aaatcagaga ctggggaact tatatttaaa ttttatagac attgcttccg ctataatgaa
    13501 attaatttga aaattcagga tatttttgat gattattctg agattcataa tccaaatggg
    13561 acacactgtt atggttacac atttaacaca gataaacacg gcaaagtgat agttgattct
    13621 atacatattc cgatgattat gagtgcatta aaagaaattg aaaagaacaa aaatgccaat
    13681 atagaagaaa aatttaatga ttctgttgaa aaatttgttc aaaaagtaaa agaaatttta
    13741 gcagatgaac caattaatga atttaaattg aagaagatgg acaaagctta tgatgagtac
    13801 ttttctgtat taaattcaaa gaaagatgga ttatttggac attatgtagc aatagaatat
    13861 gtgaaagata gtgatttacc acagccggaa tttaacagct tcttcataag tgatattgag
    13921 aaagcaagaa aatctcccaa ccaaacttta attgattaca ttgaaggtgt agaagaaagt
    13981 cagcacatag aagtagatga aaataaagaa atgtttgaca aatttttaca tccttcacgt
    14041 ttgcctgatg gacgatggcc atcacagact gagtttagat tgtctttaat gcaacaactt
    14101 gctgtaaacc aaattacgag tggtaatgaa agaataagtt cagttaatgg gccaccaggt
    14161 acaggtaaga ctactttatt aaaagatata tttgctcatc tagtagttga aagaggtaaa
    14221 gagttagcta aactaaataa tcctaaagat gcatttgtca aaacaaaaat tcatgaaacg
    14281 gatgataaat acgtatactt actaaaggaa tctattgcca aatataagat ggtagtcgca
    14341 tctagtaata atggagctgt tgaaaatata tctaaagatt taccgaaaat tgaagaaatt
    14401 ataagaaatc ccgaaaaatg taaattccct aaatatgaac agaattatgc aaatttagca
    14461 catgaattaa aagattttgc tgaaatagct gaagatttga ttggtgaaag tgcctggggc
    14521 ttattttctg gagtttttgg taaaagtact aatattaacc aagtattgag tcatatgtta
    14581 aaacaagatg cgaatgatat tggctttgct aaattactac aaaatgagaa taatcgtatg
    14641 agttataacg agttaatgag tgaatggcaa tcacatcaac gtgcattttt agaagagttg
    14701 aggcatgttg aaatgttaaa agaagaatct attagagcat atgatgttta taaaaattgt
    14761 gagtctttct ctaagattga acaggttatt aatagtgaaa aaacaagtat tgaagaacag
    14821 gtatatcatt tagataatga aacgttacga gacaataaag aaatagaaga tttggataat
    14881 cgaattaatt atattgttaa gcaaatagaa actttaaatg agttaattaa atccatcaaa
    14941 gaaagcaaca aaggttttat taacaaactg aaagcgatgt ttaattcaga agaagatgaa
    15001 agctataaag atcataataa agagaagcaa caattattaa cacaacagtt agagttagag
    15061 aaatgtaaaa aaaacaaaca tgaagacctt gttagcaaac taaaagaaaa agagaaatta
    15121 attaaacaat taactaaagt acagttgcaa ttagacgagt taaattcaca gttacaagag
    15181 ttagaagcat atcgtattga gtcaaaaatt acaattccag aaaaagattt ttggagtgac
    15241 aacaattatg atgagcgcca agttactaat ctgtggacga gtgacgaact tcaatacaga
    15301 cgtgccatgc tctttttaag agcaatgata ttgcataaat tattattgat tgctaataat
    15361 acaactattt attatgcgat taatgatttt aaagatagaa ggaaattaat tgatgcaaat
    15421 ccagataaag tacacaacgc atggaatgtg atgcatttaa tatttccagt agttagtacg
    15481 acgtttgcaa gctttaaatc tatgtatggg ggcataccaa aagatttcat agactactta
    15541 tttattgatg aagcaggaca agcaatacct caagcagctg tgggagcatt atatcgttca
    15601 aaaaaagttg tagctgtagg tgatccgatt caaatagaac cggttgtgac tttagaaagt
    15661 catttaattg ataacattcg taaaaattat catgttccgg aatatctagt ttctaaagaa
    15721 gcttctgtgc agtctgttgc agacaacgcc aatcaatatg gtttttggaa atctgatgct
    15781 actgatagta atcaaaaaac ctggataggc atacctttat gggtgcacag acgatgttta
    15841 aaacctatgt tcacgatagc taaccaaatc gcttataata ataaaatggt gttgccaagt
    15901 aatattacaa aagtaggtaa aacaggttgg tatgacgtta aaggaaacgc agttcaaaaa
    15961 caatttgtga aagagcatgg tgaaaaagta gtgggattat tagctgatga ttggattgaa
    16021 gcaattaagg aaggtaaaaa tgaaccgagc tcatttgtaa tatcgccttt ttcagcagta
    16081 cagcaacaga ttaaacgtat gttaaagcaa caactaccga ctagaattga tattgaacgt
    16141 acaaaaatta atcaatgggt cgataaatcc attggtactg ttcatacttt tcaaggtaaa
    16201 gaggctcaga aggtgtattt tgtaataggt actgataata cccaagatgg tgctgtgaac
    16261 tggtcatgcg aaaaaccaaa cttgttaaac gttgcagtga caagagctaa gaaagagttt
    16321 tatgtaattg gcgacatgca aagaatacag atgaaaccat tttatgagac gatttttaaa
    16381 gaaagaaatg taaaataaca tacaaaaaag tatatagagg aagttataca ttttaaaagg
    16441 agcaaaattg aataatggag aaatttaaca actggatatt aaatgcaata agtggatctc
    16501 aaacagacaa gaatgaaaca actgaagaat taaaaggggc aaaatttatc attttatatg
    16561 catattcaat gctcgttttg cttgcgttag taatttctaa catattcatt cacattttgg
    16621 agcctaaact atcaatcacc actcaaatca tcatcgtttt gattttaatt gaagcactaa
    16681 ttggactgcg tttcttgaaa gcgtacgatg ttaagcgtgg caaagataaa gaaaataaga
    16741 aaaatagtaa ggatttcgtt aaactaaaat caattttagt agcaatttta tttacatcat
    16801 tggcgctgac agcaggtact gtagctgata tatacggttt cactgactta ggaaatacta
    16861 gaagtgattt aatcgtttgg agcataggtg gtattatatt tggcctcgta tgttacacaa
    16921 tggaagataa aagataacga taaggagctg gcgattataa agctagctcc ttttttaact
    16981 tatatatgta aagaactatc ctaagggttt ttaatcata
//
LOCUS       DALAGF010000057        16977 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.057, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000057 DALAGF010000000
VERSION     DALAGF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16977
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.057"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(309..800)
                     /locus_tag="O2692_001672"
     CDS             complement(309..800)
                     /locus_tag="O2692_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ4299303.1"
                     /translation="MIRYAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNRWLDLAFYELD
                     LQD"
     gene            994..1614
                     /gene="srtA"
                     /locus_tag="O2692_001673"
     CDS             994..1614
                     /gene="srtA"
                     /locus_tag="O2692_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HCZ4299304.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVGVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            complement(1774..2505)
                     /locus_tag="O2692_001674"
     CDS             complement(1774..2505)
                     /locus_tag="O2692_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HCZ4299305.1"
                     /translation="MSTLKLEFKKSISNKIIYTLVVLFTFLFLLGYFLPIGIDKVKSL
                     SYSQFFFSSYTVATQLGFLLFSFVIAYFINKEYSNKNILFYKLIGDNIFTFFYKKVAV
                     LFFECLVFIILSITIISIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISL
                     GISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTFMCPTEIINIVS
                     FFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(2511..3146)
                     /locus_tag="O2692_001675"
     CDS             complement(2511..3146)
                     /locus_tag="O2692_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ4299306.1"
                     /translation="MKLNNYSLKVKNKQLVDNCDLNFYLGQINHIVGKNGVGKSLLAK
                     DFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSKFENNRQTFDKIYNIL
                     NIEAIPSNVLLKNLSDGQKQKLKLLSFLLEDHDLIILDEVTNALDKKTVNEIYEFLND
                     FIQSHQTKTIINITHNLSDLSALPGKYFIFKDLQIEEYQSKEEVINDYINL"
     gene            3467..4267
                     /locus_tag="O2692_001676"
     CDS             3467..4267
                     /locus_tag="O2692_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ4299307.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKLRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYNGNELYNYKSFNRQVFQQVVDYLNMKQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGNDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK"
     gene            complement(4516..5508)
                     /locus_tag="O2692_001677"
     CDS             complement(4516..5508)
                     /locus_tag="O2692_001677"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCZ4299308.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            5778..6449
                     /locus_tag="O2692_001678"
     CDS             5778..6449
                     /locus_tag="O2692_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCZ4299309.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQEPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            complement(6569..7375)
                     /locus_tag="O2692_001679"
     CDS             complement(6569..7375)
                     /locus_tag="O2692_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4299310.1"
                     /translation="MAFHDKTATQVTNIVLNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNSQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(7546..7830)
                     /locus_tag="O2692_001680"
     CDS             complement(7546..7830)
                     /locus_tag="O2692_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ4299311.1"
                     /translation="MSNLEIKQGENKFYIGDDENNALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLLKAVVEHARENNLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            8057..8491
                     /locus_tag="O2692_001681"
     CDS             8057..8491
                     /locus_tag="O2692_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ4299312.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            8569..9537
                     /locus_tag="O2692_001682"
     CDS             8569..9537
                     /locus_tag="O2692_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCZ4299313.1"
                     /translation="MINNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALTYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEVRPFNTKRQHG"
     gene            9559..10152
                     /locus_tag="O2692_001683"
     CDS             9559..10152
                     /locus_tag="O2692_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4299314.1"
                     /translation="MEHIFREGQNGAPTLILLHGTGGDEFDLLLLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLAFRGQELLTFIKEAAERYDFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFNTRGAQVEEVWVKGHEITETGLTAGQQILGK"
     gene            complement(10257..11336)
                     /locus_tag="O2692_001684"
     CDS             complement(10257..11336)
                     /locus_tag="O2692_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299315.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(11680..13644)
                     /locus_tag="O2692_001685"
     CDS             complement(11680..13644)
                     /locus_tag="O2692_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HCZ4299316.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQNCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKDLQAQIDILKMLMHDRYLD"
     gene            complement(14017..14787)
                     /locus_tag="O2692_001686"
     CDS             complement(14017..14787)
                     /locus_tag="O2692_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4299317.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            complement(14789..15484)
                     /locus_tag="O2692_001687"
     CDS             complement(14789..15484)
                     /locus_tag="O2692_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ4299318.1"
                     /translation="MDVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNKPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLEIIEREDQA"
     gene            15918..16376
                     /locus_tag="O2692_001688"
     CDS             15918..16376
                     /locus_tag="O2692_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ4299319.1"
                     /translation="MKREVILKLQQFIIERENADKRRQYKKENEELDLSLTQFHIIEL
                     IDNNDKVNNKFLSEMLNVSKPAITKSIKKLLAKDLVVESHNEFNKREVNYSLTQKGKK
                     LSYIHDELHEKSVKKYEEVLKVFDDDEMAVIIEFLNRSIEELKKEDGLND"
     gene            16369..>16977
                     /locus_tag="O2692_001689"
     CDS             16369..>16977
                     /locus_tag="O2692_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4299320.1"
                     /translation="MINSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWIN
                     ATVGGFIFIVAVMQIVLGRSIDSRDSKKVLLTGLGIVIISSFICAVTHNFILFAISRL
                     FQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPALAPILGSTLGARWDYI
                     GIFSFLLVISIVLFLIIFFIDIPNVEKGIVKLTEKIEEKYLTD"
BASE COUNT         5918 a         2533 c         2952 g         5574 t
ORIGIN      
        1 ggccccaaca cagaagctga cgaaaagtca gcttacaata atgtgcgagt tggggtggga
       61 cgacgataaa gaaatacttt ttctatagaa attagtattt cttatgcatg agttttactc
      121 atgtattcct atttttaagt acacattagc tgtggctaat gtgtaagaac cactacataa
      181 taaatcatta gtggctcttt atcatttctg tcccactccc ctgagaagtt taaaatttta
      241 tatgttggct tgttatgtta agggaattaa catggttgtc ttgtttatat tatgtgattc
      301 aaacattact agtcttgtaa atctaattcg taaaatgcta aatctaacca tctattaaat
      361 ttaaaaccta cattggtcag tgtgccggca tgcttaaaag caaacttttg atgcaactga
      421 atactcgctt tgttggaagc atcaatgcct gcaactagcg cacgataacc tttagctttt
      481 gcttccacaa ttaaatggtg tagtaattga ctagcaatac cttttcctct agctgaagca
      541 tcgacataaa tagaatgttc gattgtatat aggtatgctg gccaaggtct aaatgaaccg
      601 aacgtcgcaa accctaagac acttccattt tcctcaaata caaagatagg ctcatgctta
      661 cgttgtttcg tttcaaacca tgcgacacgt tcgtctatgg tttgtggttc ataagtataa
      721 acagctgtag tattgataat ggcatcattg tatatcgcta atatagcgtt taaatcctct
      781 tttttagcgt atctaatcat atcaattccc ccttagtaat tattaaaagc gtttcgttat
      841 ttgaatgcaa atatgtgtaa tgaaatctaa cgtaaaagta tacatgtaaa ttttatagta
      901 taaaatgaat tgctatgagt cattttgaaa ttaatggtat actatatgaa atgttaacag
      961 gcattgtgaa atgtataaaa ggagccttaa cgtatgaaaa aatggacaaa tcgattaatg
     1021 acaatcgctg gtgtggtact tatcctagtg gcagcatatt tgtttgctaa accacatatc
     1081 gataattatc ttcacgataa agataaagat gaaaagattg aacaatatga taaaaatgta
     1141 aaagaacagg cgagtaaaga taaaaagcag caagctaaac ctcaaattcc gaaagataaa
     1201 tcgaaagtgg caggctatat tgaaattcca gatgctgata ttaaagaacc agtatatcca
     1261 ggaccagcaa cacctgaaca attaaataga ggtgtaagct ttgcagaaga aaatgaatca
     1321 ctagatgatc aaaatatttc aattgcagga cacactttca ttgaccgtcc gaactatcaa
     1381 tttacaaatc ttaaagcagc caaaaaaggt agtatggtgt actttaaagt tggtaatgaa
     1441 acacgtaagt ataaaatgac aagtataaga gatgttaagc ctacagatgt aggagttcta
     1501 gatgaacaaa aaggtaaaga taaacaatta acattaatta cttgtgatga ttacaatgaa
     1561 aagacaggcg tttgggaaaa acgtaaaatc tttgtagcta cagaagtcaa ataatctatt
     1621 acgctaatgg atgaatatat tgagtggaaa acagtcttga ttgcgagact gttttttgtt
     1681 tggtatgagg tagcaatgac gacgtgtcat tggtggagat tgtaaaaata cataataaaa
     1741 agaagcggca atgtataccg ctcctttttt atactacata ccgattttca accatctctt
     1801 tctacttagt aataagacaa tagtattaac tataaataga agaacgaaga atgatactat
     1861 atttataatt tcagtaggac acataaatgt tgactcgtta ttcaatattt tttctacggc
     1921 acgatacatc gtattgctcg cctcaaatgg agcaacgata ccaaatatat ttttattaat
     1981 ggcaactaag atgactgaac caatccaata tacaatgctg atacctaagc tgattaaaat
     2041 gttaggtgaa accatactaa tcgttccaac aactaagata tattgtaaga taacgagtga
     2101 aaataagatt attaatagta agtaatgtga gaaatccgaa tatataattg aaataatagt
     2161 gatacttaga attatgaaca ctaaacattc aaaaaataac actgctacct ttttatagaa
     2221 gaaggtaaag atattatcgc caatcaattt ataaaacagg atatttttat tcgaatactc
     2281 tttattaata aaatatgcaa taacaaatga aaatagtaag aaccctaatt gcgttgcaac
     2341 agtatatgaa ctgaagaaaa actggctata gcttaaactt ttaactttgt ctatacctat
     2401 tggtaaaaaa tacccaagta agaaaaggaa tgtgaatagc acaacaagcg tgtaaataat
     2461 tttattggaa atactttttt taaattctaa tttcaaagtg gacacctcaa ttataaatta
     2521 atgtaatcat ttatgacttc ttcttttgat tggtactctt ctatttgaag gtctttaaaa
     2581 ataaagtatt tacccggcaa agcacttaaa tcggataaat tatgtgtaat attgataata
     2641 gttttagttt gatggctttg aataaaatca tttaaaaatt cataaatttc attaactgtt
     2701 ttcttgtcta aagcgtttgt aacttcatct aatatgatta aatcatgatc ttccaataag
     2761 aaacttaata atttaagctt ttgtttttga ccatcactca agttttttag tagtacgtta
     2821 gacggtattg cttcgatgtt tagtatgtta taaatcttat cgaatgtttg tcgattgttt
     2881 tcaaattttg attttaacaa tgataataaa aaatcttttg ttatatcatt aggaatattt
     2941 gatgaacttg atattaaggt tacattttga gaaatggact tagggatatt tccactatta
     3001 tttagtaaga aatctttagc taataaagat tttcctacac catttttacc aacaatgtga
     3061 ttgatctgac caagatagaa atttaaatca caattgtcaa ctagttgttt gtttttaact
     3121 tttaaagaat agttatttag tttcatgtat acaactccta tgtataaagg gatttattac
     3181 accgatattt aattgtattt ttaaaaattc tttcacatta tgtatagatg ttataaatta
     3241 gtatatcaca ctatattttg tctaaatgat aaatatagtg tgatattttt acaatattct
     3301 gaaatttatg ttcgcctctg aatgctatat ccagtgtaat gtgttttgca tatatgaaag
     3361 caatttcaaa atgtgaatat aggttcattg tggtatgaca aacttcattg cttgtcatga
     3421 gatggatata atgaagacga ctaaaagagc aatgaggagg ataatcatgg ttaaggctat
     3481 tgcggtagat atggacggga cgtttttaga ttcaaaaaag acatatgata aacttagatt
     3541 tgaagcgatt tttactgaac ttagaaatag agatattaca tttattgctg cgagtggcaa
     3601 tcaatatgcg aagttgaagt ctattttcgg ggatagagat atgtatttta tttctgaaaa
     3661 tggtgcagtt atttataatg gcaatgagtt atataattat aaaagcttta atcgtcaggt
     3721 gtttcaacag gttgtcgatt acttaaatat gaagcaaagt attgatcaac tcgtcatctg
     3781 tggtttgaaa agcgcgtata ttttaaaaca tacttctgaa gcgtttaaag aagatacgag
     3841 attttattat catcagttaa aagaaattga cagtctacag caattacctg aggatgatta
     3901 tgtcaaaata gcatttaata ttaatcgtga gacgcatccg aatgttgacg aagaagtagc
     3961 aacgcaattc agcaatgata ttaaacttgt ctcaagtggg aacgatagca ttgatattat
     4021 tatgccaaac atgactaaag ggcaggcatt aaaacgattg ttagataaat gggaaatgtc
     4081 tccttcagag ctcatggcct tcggagatgc gaataatgat aaagatatgt tagcgttcgc
     4141 taagcatagt tatgtgatgg aaaatagtca tgatgaagaa ttgttcaata ttgcctctgc
     4201 tgtcgctcca agtaatgata agcagggtgt attaacgatt atcgaacaag aagtgttgaa
     4261 aaagtaggta gtatatcatc aatgaagcag ctatcatgat actaaataca tgttaaatga
     4321 aaactttgaa tacgaatcat caaatattaa tatatctcaa acaggttagg caataatgcc
     4381 atagtaaaat aagcgcacta aaatcatttg aatagatgat aaaataaagc ggattttaat
     4441 caaatcgatt ttgatacaga tatacaaaat aaaaagcaaa ggcacatggc gcgcctttgc
     4501 ttttaaatat atatattaat ttaaacgtgt ttcacatgta ccagtgttaa tgacagataa
     4561 tgctgcgttt aaaccacctt caacaaggtt ttgtactgct tcatcagaga agaaagcaat
     4621 atgtggtgtt actaaaattc tttcatgttc gattaactct aataatgttt tatcgtcaat
     4681 gtctttatta gtccagtcat ttgtgaagta tgctgcttca ttttcataag tatcaatcgc
     4741 agcacctaac aaagtaccat cgttcactgc agcgattaaa tcaggtgtat tgatgactgc
     4801 accacgtgct gcgttaacta agattgcacc ttttttcaca tgatcaaaca ttgctttatc
     4861 gaataaatga tagctttctt tgttcgctgg aacatgtaaa gaaataatat cggcatcttt
     4921 aatagcttct ttaacactat ctttataagt taaaaagtct aaatctttat taggataggc
     4981 gtcataagct gtaattgtag caccaaatcc tgcatatatt ttagctgtag cagcaccgat
     5041 acgacccgta ccaataattg caacagtcat atttttaact ggtttagaca tgatttctgc
     5101 ttgccaagta aaatcatgtg cttgtacacg gcgttcaata tctgggaagc gacgcactaa
     5161 ttgtagggcg atagaaacag aatactctgc aattgtttca ggtgaataac taggaacgtt
     5221 agatatcaca atattgtgtt ttttagctaa atctaaatca tacatatcaa atccagcagt
     5281 acgttgtgca atttgtttaa taccgtaaga ttctaattta ggataaacgt cattttctaa
     5341 cttaccaaat tgcattgtag ttacgccatc gtaatctttt aattgatcga ctgtagcact
     5401 tgataatagc tctttagaag tagttacttc gacattattc tttttccccc aatttaatgc
     5461 catctctttc tcataatcac gcgtaccaaa gaacataatt tttgtcatta ttaaaaacct
     5521 cgcttttaaa agattgaaaa gtaaatgagt gaaattaaag attatgcaca ttaaaatcac
     5581 gccacaattt aattgtgaaa aatatcacaa atatattata acactaaatt tcccaaaatt
     5641 caaaagtgtg ttttattgca gaaaacttat aacatgtgca caagttatag tgaattgcaa
     5701 acggattact ttagtctttt taaaacatga agtataattt gtatagcaat aaatataaaa
     5761 atgggaggct atgttcaatg agcaatatga atcaaacaat tatggatgca tttcatttca
     5821 gacatgcgac taagcaattc gatccacaaa agaaagtttc gaaagaagat tttgaaacaa
     5881 tattagagtc aggtagattg tctccaagtt ctcttgggtt agaaccttgg aagtttgtcg
     5941 tgattcaaga tcaagcgtta cgtgatgaat taaaagcgca cagttggggc gcagcaaaac
     6001 aattagatac agcgagccat tttgtgctaa tttttgcgcg taaaaatgta acgtcaagat
     6061 caccgtatgt acaacatatg ttaagagata ttaaaaaata tgaggcacaa acgattccag
     6121 ctgttgaaca aaaattcgat gcattccaag cagatttcca tatttctgat aatgatcaag
     6181 ccttgtatga ctggtcaagt aaacaaacgt atattgcatt aggcaatatg atgacgacag
     6241 ccgcattgtt aggtattgat tcatgtccga tggaaggttt tagtctggat acagtgacag
     6301 acattttagc aaataaaggg atcttagata ctgagcaatt tggtttatca gtgatggtcg
     6361 catttggcta cagacaacaa gagccaccga aaaataaaac acgccaagct tatgaagatg
     6421 ttattgaatg ggttggacca aaagaataaa tagaataccg tatgtctaaa tatataaaat
     6481 taaaaagtta gcaataaaaa agcctgcgat tacataaatg aatcgcaggc ttttgcgtga
     6541 aaaaattgta ttaataaagt atggatgatt atttttctgg aacaaggtca gtatttgaat
     6601 gaactgtgat gtcaaaccct tctggtgccg taaatgtatg tgttgaggcg tcgggttgat
     6661 aaatatcaac atgtgttaat ccataacttt gtgaattgtt ttgtcttgct tgattggatt
     6721 gccaagtatt agcagcaata tgatggtgat aatgattcgt tgacataaat agcgcacgtg
     6781 gaaaatcaga cacatgttgg aatcctaatt gttcaatgta acattgatat gctgcgtcta
     6841 aatcatgtgt ttttaaatgt aagtgtccaa tcatgccttt tgctggcatt ccttgccaac
     6901 cttcatcagt acgatgtgtt aataaggttt ggctatcaac ttctaaagta tccattttaa
     6961 ctttgccatt ttgccattcc catgaagatg aaggtctatc gcgatagact tcaataccat
     7021 taccttcggg gtcgttgaaa tataaagctt cacttactaa atgatcacca gcgccgatgc
     7081 ccatattttt ttgtgccacg aaatataaga agttagctag atcctcagta gttggtaata
     7141 aaaatgctat atgaaaaagc cctgcttcac gtggggaagt ctgacggccg tcttctaata
     7201 aatgtaacgt tagagtatgg ccaccagtcc caacagataa tacggttgta ttatcgtcag
     7261 aacttttaac ggatagtcct aaaatgtttt tgtaaaatgt tgtcattaag tctaagtctc
     7321 ttacgttcag tacaatgttt gtcacttgtg ttgctgtttt atcgtgaaat gccattatgc
     7381 atcgcctctt tttctatttt tctataagtt agtataaaaa gtataccaga aaagaaaatg
     7441 aattgatagc ataaagtttg aaatgcaaaa taactagtcg ttttgcaatt ttatattgat
     7501 gcgaacaaaa aagcgatggt acagttgcac catcgcaaaa tttatttaac caagatatac
     7561 atcttgatat gaatcttctt tttctaacat atgtttggca aatgaacatg aggcaataat
     7621 tttcaaatta ttttctcgag cgtgttcaac aactgcttta agtagttttt tgccaacacc
     7681 ttgaccacca agttcatcag atacgcctgt atgatcaatg ttaatttcat tattatccac
     7741 aaaacggtat gtgatttcag ctaaagcatt attttcatca tcaccaatat agaatttgtt
     7801 ctcgccttgt ttgatttcaa ggttactcat acatatcaac tcctatcatg attgattata
     7861 gtatttccct attctatttt aacttaaacg aagtcaaagg tgcatgacag tcatgtgacg
     7921 acattgccac atctatgtag tcgtttttat taagcacagt ttgaaatgaa gatgaaaaca
     7981 cgtatcttga cattaaatct attcagctat ataatttatc tcgaaatcga aataaaataa
     8041 aaaagttggt gatcatatgg atcgaacgaa acaatctctc aatgtttttg tcggaatgaa
     8101 tagggcgtta gacacattag agcaaattac aaaagaagac gtaaagcgat atggcttaaa
     8161 tattactgaa tttgcagtgc tcgagttgct ttataataaa ggtccgcaac caattcaacg
     8221 tattagagac cgcgtattaa ttgcaagtag cagcatttca tatgttgtaa gtcaattaga
     8281 ggacaaaggt tggattacac gtgaaaagga taaagatgat aaacgtgtat atatggcttg
     8341 tttaactgaa aaaggtcaaa gtcaaatggc agatattttc cctaagcatg ctgagacatt
     8401 aacaaaagcg tttgatgtgt taacaaagga tgaattaaca atcttacaac aagcgtttaa
     8461 gaaactaagt gcacaatcta cagaagtgta aggcgtgcac taaaaattta cattaaagta
     8521 tctcgatttc gagataaatg cactaaaaat ataaagaggg tatataaaat gataaataat
     8581 catgaattac taggtattca ccatgttact gcaatgacag atgatgcaga acgtaattat
     8641 aaatttttta cagaagtact aggcatgcgt ttagttaaaa agacagtcaa tcaagatgat
     8701 atttatacgt atcatacttt ttttgcagat gatgtaggtt cggcaggtac agacatgacg
     8761 ttctttgatt ttccaaatat tacaaaaggg caggcaggaa caaattccat tacaagaccg
     8821 tcttttagag tgcctaacga tgacgcatta acatattatg aacagcgctt tgatgagttt
     8881 ggtgttaaac acgaaggtat tcaagaatta tttggtaaaa aagtgttgcc atttgaagaa
     8941 gtcgatggcc aagtgtatca attaatttca gatgagttaa atgaaggggt agcacctggt
     9001 gtaccttgga agaatggacc ggttccagta gataaagcga tttatggatt aggccccatt
     9061 gaaattaaag taagttattt tgacgacttt aaaaatattt tagagactgt ttacggtatg
     9121 acaactattg cgcatgaaga taatgtcgca ttacttgaag ttggcgaagg aggcaatggt
     9181 ggccaggtaa tcttaataaa agatgataaa gggccagcag cacgtcaagg ttatggtgag
     9241 gtacatcatg tgtcatttcg tgtgaaagat catgatgcaa tagaagcgtg ggcaacgaaa
     9301 tataaagagg taggtattaa taactcaggc atcgttaatc gtttctattt tgaagcatta
     9361 tatgcacgtg tggggcatat tttaatagaa atttcaacag atggaccagg atttatggaa
     9421 gatgaacctt atgaaacatt aggcgaaggg ttatccttac caccattttt agaaaataaa
     9481 agagaatata ttgaatcgga agttagacct tttaatacga agcgtcaaca tggttaattg
     9541 gaatgaggag gatttgtgat ggaacatatt tttagagaag gacaaaatgg tgcgccaaca
     9601 ctaatattat tgcatggtac aggtggtgat gagttcgatt tattactgtt aggcgaagca
     9661 ttgaatgaaa attatcactt gttaagtatt agaggacaag tttcagaaaa tgggatgaac
     9721 cgttatttca aacgtcttgg tgaaggtgtt tatgatgaag aagatttggc atttcgtgga
     9781 caagaattgt tgacgttcat taaagaagct gctgaacgtt atgattttga tattgaaaaa
     9841 gcagtacttg ttggattttc aaatggatca aatatagcga ttaacttaat gttgcgttca
     9901 gaagcaccat ttaaaaaagc attgttatat gcaccgttat acccagttga agtaacgtca
     9961 acaaaggatt tatcagatgt cagtgtgttg ctttctatgg ggaaacatga tccaattgtg
    10021 ccattagctg caagtgaaca agtcattaac ttgtttaata cacgtggggc acaagtcgaa
    10081 gaagtttggg tgaagggcca tgaaattaca gaaactggat taacggctgg tcaacaaata
    10141 cttgggaaat aacagttcta ttaagaagcg gacagatgga aaagattttt acttttcatc
    10201 tgcccgcttt tttgattttg aagtgctgta ctaaatttta caatagtata gatattttaa
    10261 tcgatatgag atttgccggt aatacgctta attaaacctt tatagagtac aggtatgagt
    10321 aagatgaaac cgaacaatcc cataataggg aatacttttc caattaatga aatgaaaccg
    10381 ataaatgtac taatataagt gatgacagcc attgtaataa taatgatgaa gtaacgtctg
    10441 ctgaatggaa cgctgaaacg tgacgcaaat gcatacatta atccaacaac agtattgtag
    10501 atgacaagta tcataatgac agacataata ataccaattg acggagacat ttgtgtcgct
    10561 aattttaatg taggtagatc tacgtgttta attttatcga attgagaaat taaacctaga
    10621 ttaatcatca tgagtaaaaa tgtaatgatt aaaccgccaa tcaagccccc gtataacgtt
    10681 gagtcacgat atttaacttt actacccatc actgataaga agctgaaggc agcagcaatt
    10741 tgcaagcttg catagttaat cgcatcaaac caccatccag gtgataatga tttctgctta
    10801 tgaatctgag catcattatt agcggcagta aaatcaagat gacttgttgt gaaatagtag
    10861 accgcaatca taatgacaat cgcaattaaa aatggggtaa caccgccaag cacagcaatt
    10921 aaacgatcga attttagaaa cagtgttgct aaaataaagg cgactaatat gagtgcgctc
    10981 agccaatacg gtaagttgaa actttgatga atggttgacg caccacctgc agtcataata
    11041 atagctaaag acaacataaa cattgttaaa ataatatcaa aacctcttgc aatagagggg
    11101 tataagaaat agttaattga atcagaatga tttctggact ttagatgatg acctgtatgc
    11161 atgacaacca ttccacctaa agtaatcaat agtcctgtta caataatgcc tgaaatgcta
    11221 tatgcgccat gacttgtgaa aaactggaaa atttcttgac cagtagcaaa gccggcacca
    11281 acgacaacac caacaaaggc aaatgccaca ataatggact cttttaagat acgcatgatt
    11341 taaaaatgtc ccttcgtaat tttaagtaat atagaaaatg taacatacat gttaatgaaa
    11401 aatatagtac taatatagta ttttgttaaa ttggagtaga agcgagggtg tcggtcattt
    11461 cattaattta ttagttgatt ttgcattttt ttgctgtaaa gttgttataa tacagttaac
    11521 aggaattagc atagatacac caatcccctc actactcgca atagtgaggg gatttttttc
    11581 ggtgtagcta ggtcgcctat ttatcatcgt gtttgcgtag ccaatgcgta aacacagtac
    11641 cactaaataa gtgcacgata catgcatcaa atgtcgtctt tagtctaagt aacgatcatg
    11701 cattaacatt ttcaaaatat ctatttgagc ttgaagatct ttaccaatat tggtatcacg
    11761 aatcttctta cgttgtaatt ctttatctac gacgcgcttt atagaaagtt catcgatacc
    11821 ttcggaaagt attttttctt tagcgttaaa ttgttggtgt gcaacgagtt gcataccgaa
    11881 tgaattatac aatagtgtat agcctgcaat gccagttgtt gactgataag cttttgaaaa
    11941 gccaccatca atgacaagca tctttccatc agccttgata ggatcttcgc cattgatttc
    12001 tttcactggt gtgtgaccat taataatgcg tccttcatct ggatttaatc cgaaatcact
    12061 gagcatttta cgaaccatat tcacatcttc acgaagatga tagtacggat tcttttcttc
    12121 tttatgagaa gctttatctg caataaagta tcgctcaaac gtagtcatgg cacgtttacc
    12181 aaatagtgac gaatatttcc cagtccataa ataccaaact aaatccgtcg ataagtcatc
    12241 agtattttct ttttcatcaa atgatttacg gacatgatac tcaaacacat ctaataattc
    12301 ttggccgctg taagtatgac catcaatttc aaatgattcc atctcaccat tttcatcaac
    12361 tggaatacaa ccatgaatga gtaaattgcc attatatggt aagtaaagag agcctttacg
    12421 catcaagaaa gacatatgac gacgtaattt ttcagattgt tggaatgaca ataatagttt
    12481 attcatgact tcttcttctt caggtagtaa ttctgctgga ttatcacgat tgacagtttg
    12541 gaaacatgtg tctttcaatg ggtatgtatt accataaact gtaatttcat ttgtatcata
    12601 attaaccttt tcaagcacaa gacgttcttc catttcgaaa tttggacgac gtttaataat
    12661 tggtatttct aacttgaatt gaatcatcgc aatagcttga tgaattttag taatttgact
    12721 ttcttcacgt tgagttaaac gttcgtgttt gtcaggtctt tttttaggct taaaagcagg
    12781 attatctgcg tcatagtatt tttcagctaa agtaagcagt ggtcttaaat taatgccata
    12841 agcgtcttcg ataatatcta aattatcata acgtgcacaa attcgaagta agtttgctaa
    12901 gcatactttt gacccagcat aggctccaac ccaaagcaca tcatgattac cccattgaat
    12961 atctagggaa tgataattaa tcagtgtatc cataatttta tctggttgtg gtccacgatc
    13021 ataaatatca ccgacaacgt gtaaatgatc gacgactaag cgttgtacgg aataagcgag
    13081 tccaataatt aaatcatctg cctgtttaag ttcaattact tggttaacaa gtgtttcgta
    13141 atatgatttt ttattttgat attcattact tttatacagt agttcctcaa taatataaac
    13201 gtattgtttt ggcaatgctt tacgcagttt tgaacgcgta tattttgaag aacaatattt
    13261 aattaactca attaaatgtt cgattgttgt gatataccag acattaagtt gaccgcaatt
    13321 ttggaaatca cttttaatca attttaattt gtcttctgga tagtagacaa gagcagttaa
    13381 gtcattaagc tccttagttg aaagtctctc tttgaaaata tcattgattt tcgctcgcac
    13441 gttcccagaa ccgttgcgta atacgtgttg gaaagcttca tattcaccat gtaaatcgct
    13501 gacgaaatgt tccgtacctt taggtaattc taaaattgat tctaaattga taatttcagt
    13561 tgcaagtttt tctggagtat caaaattttg ggatagtaaa tctaaatact tcttttttaa
    13621 ttctttttca gtaatttgag tcattacgtg tcactcctgt agtatatttc aaacgttagt
    13681 ttataaaata aatgcgctta caatgattat caagaaaacg ttaacatttt tcaataagta
    13741 tgcttgaatt gataaaaata gagggcagga ttttggtatt aggaaccgtt gcagtttagt
    13801 taagtaaaag tggggaagtg atatatctat tttaaaaatg agaggaggga tttgttatat
    13861 tcactagttt cctaaaaaat tacgatatgt aaaatgtgga atttccaaaa tttggatgaa
    13921 aaaatccccg caccattatt tgggtgaagg gatttgaaaa tgattttgaa ttacgatgga
    13981 atatgtgtgt atttagacat ttaatacttt aagttactaa atatttttct tcttgaaaat
    14041 aaatactaca agtattaaga aaatagaaat ataagcaata ttaccaataa acaattcatg
    14101 aagttctaat tttgtcgact tactaaagcc tttttcaact gtttgaagca taatattcat
    14161 catattaata ggattccact ttagccagtc tattttttct aaaagtgccg tttgaataac
    14221 tgctaaaata gaacttgcaa aataaaaaac aatacctaca gcaatggcta ctcccgttga
    14281 atttgttgca gatgataata gcaacgttaa gcttaaaact aaccaaacgc caacaaatgt
    14341 gcctaaacta actaataata attgattcaa taaagataat tgattaccgc tactttcaaa
    14401 tatatttaaa tcattaaaga ataaagaccc aataacaatt gaagcaataa ttgtaataac
    14461 aaaataaatt aaagaaataa taaataatgt gatgattttg ctaacaatca tagttgttct
    14521 tgaatattca cgatagagta aatttttaat cgtaccgtaa tgaaattcca ttgaaatgat
    14581 tgtacttgct tgaataatta ataaaaatgc aaaccatgaa aaaccattat aagcagatgt
    14641 gaaagattcc tgtggcgtaa aaatttcatt gtattttgta gcaatataac cttgagcaac
    14701 cattagtaga ataaagacaa taggtgcgat aaaagttgat ttctttttaa ataatttgaa
    14761 acattcttgt ttaattaaag ttcccatttt atgcttggtc ctccctttct atgatttcta
    14821 gtaatacatt ttctaaatca ttatctttaa attgtttaag ttcttcttcc gatgtttctg
    14881 taacaatttt acctttgtta ataataagga tagagtttgt gattttaact aattcactta
    14941 aaatatgact cgaaattaag aaagtaacac cttcttgcgc tttttggaca atcaattcac
    15001 gtacatctcg cacagctttt ggatctaagc cattcattgg ttcatctaag ataatgaatt
    15061 gaggtttatt taaaaatgct atagcaattc ctaatttttg tttcatacca agagaatacg
    15121 ttttagcttt tttatgaatg tattcatcca tatgaagttg tgagacaatt ttatcgatat
    15181 cttgagtgtt ttttgattca ttcaataact tcaagttttc atatccagac ataaaaggat
    15241 atattcctgg attttcaatc aatgcgccga tattgctttt gctgtccttg ctgttaataa
    15301 cattaaaatt cccgctttgg aaactagagt aacctaatat aactttcatt aaagttgttt
    15361 tacctgcacc attcgctcca acgagaccaa ctatttgtcc gcgtttcagc ttaaatgata
    15421 catcttcgag aatcgttttg ttgcctatct tctttgttaa atgttctatt gttaaaacat
    15481 ccatatcact atctcctttg ctattaaata atctgaatct taagatttta tatataaaat
    15541 taattttatc acatagcaag tgttataaaa atatggattt aaaaaacttt aataatataa
    15601 aacataaata tgaatacgag tatttttgca gcatgcaaat ggcattgcta catttattaa
    15661 aatgcgcaat ttgttatgag taactttttt ggatagtagg cacattaaag tgtcgaaaac
    15721 aatttgtgtg gaagacaggg taaattctgc ttatttaaaa tgatagtttg tattataggt
    15781 ggatttgtga aatgcggaat aaatggtgca taactaagta aatatatgtt aattacaaca
    15841 atatttgaca aaaatgggag tgacaacaat aatatagtta accggttaac taatgaagag
    15901 gaggaataat agtggatatg aagagagaag ttattttaaa attacaacaa tttattatag
    15961 aaagagaaaa tgccgataaa agaagacagt ataaaaagga aaatgaggaa ctagatttat
    16021 ctctaacaca atttcatatc atagagttga ttgataataa tgataaagtg aataataaat
    16081 ttttgtctga aatgttaaat gtatcaaaac cagcaattac taaatcgata aaaaaacttt
    16141 tagcgaaaga cttagtagtt gaatcgcaca atgaatttaa caaaagagaa gttaattatt
    16201 cattaacaca aaaaggaaaa aaattatctt atatacatga tgaattacat gaaaaatcgg
    16261 taaaaaaata tgaagaggta cttaaagtct ttgatgatga tgaaatggca gttattatag
    16321 agtttttaaa ccgtagtata gaagaattaa aaaaagaaga tggtttaaat gattaatagt
    16381 aatatcagat tatatattct aggattttta gcatttttcg catcattgat tcaaaacata
    16441 tacacgccta ttattccgag actatatgac gattttcaag taccactatt ttggataaat
    16501 gcgacagttg gcggttttat atttatcgtt gcagttatgc aaattgtact tggtagaagt
    16561 atagattctc gtgattcgaa aaaagtactt ttaacgggat tgggaatagt aatcatatct
    16621 agctttattt gtgcagtgac acataacttt atattatttg ctatatcaag gttatttcaa
    16681 gcaattggtt gcggaattat tcctctcgtg actttaactt tattagccaa actaagtaca
    16741 gataatggta gagctcaagc tatggcgaac tatcaaatat ttttatcatg tgcaccagct
    16801 ttggcaccaa tcttagggag tactttaggt gcaagatggg attatattgg tatttttagt
    16861 tttttgctcg ttatatctat cgtattattt ttgattatct tttttattga tattccaaat
    16921 gttgaaaaag ggattgtaaa attaactgaa aaaattgaag aaaaatattt gaccgat
//
LOCUS       DALAGF010000058        16603 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.058, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000058 DALAGF010000000
VERSION     DALAGF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16603
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.058"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            222..950
                     /locus_tag="O2692_001690"
     CDS             222..950
                     /locus_tag="O2692_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HCZ4299321.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSGLDEV"
     gene            1035..1288
                     /locus_tag="O2692_001691"
                     /pseudo
     CDS             1035..1288
                     /locus_tag="O2692_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1304..1627
                     /locus_tag="O2692_001692"
     CDS             1304..1627
                     /locus_tag="O2692_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299322.1"
                     /translation="MNNEELEMRLLLMKQSIEQLQEELAPNLKTRDLVLLRYMYSYKE
                     INMLDSYLFQLATNKEQITKKQFKTKLENIREVPEIPIRQVNDILEGYKNSELYVELI
                     NSILK"
     gene            complement(2497..2858)
                     /gene="ssrA"
                     /locus_tag="O2692_001693"
     tmRNA           complement(2497..2858)
                     /gene="ssrA"
                     /locus_tag="O2692_001693"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2949..3413)
                     /gene="smpB"
                     /locus_tag="O2692_001694"
     CDS             complement(2949..3413)
                     /gene="smpB"
                     /locus_tag="O2692_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCZ4299323.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            complement(3435..5807)
                     /gene="rnr"
                     /locus_tag="O2692_001695"
     CDS             complement(3435..5807)
                     /gene="rnr"
                     /locus_tag="O2692_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCZ4299324.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSYRGQSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEGSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPNVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     NEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGKQRKGKNQRNNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            complement(5841..6581)
                     /locus_tag="O2692_001696"
     CDS             complement(5841..6581)
                     /locus_tag="O2692_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCZ4299325.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPDEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDNEMDHFKPTET
                     LKELSEALDTIKEQVDEVLDPILVIQAENDNMIDPQSANYIYDHVDSDDKNIKWYSES
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            complement(6697..6930)
                     /gene="secG"
                     /locus_tag="O2692_001697"
     CDS             complement(6697..6930)
                     /gene="secG"
                     /locus_tag="O2692_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCZ4299326.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            complement(6997..7455)
                     /locus_tag="O2692_001698"
     CDS             complement(6997..7455)
                     /locus_tag="O2692_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299327.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            complement(7795..9099)
                     /gene="eno"
                     /locus_tag="O2692_001699"
     CDS             complement(7795..9099)
                     /gene="eno"
                     /locus_tag="O2692_001699"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCZ4299328.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            complement(9229..10746)
                     /locus_tag="O2692_001700"
     CDS             complement(9229..10746)
                     /locus_tag="O2692_001700"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCZ4299329.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            complement(10749..11510)
                     /locus_tag="O2692_001701"
     CDS             complement(10749..11510)
                     /locus_tag="O2692_001701"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCZ4299330.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            complement(11632..12822)
                     /locus_tag="O2692_001702"
     CDS             complement(11632..12822)
                     /locus_tag="O2692_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCZ4299331.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            complement(12961..13971)
                     /gene="gap"
                     /locus_tag="O2692_001703"
     CDS             complement(12961..13971)
                     /gene="gap"
                     /locus_tag="O2692_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCZ4299332.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            complement(14024..15037)
                     /locus_tag="O2692_001704"
     CDS             complement(14024..15037)
                     /locus_tag="O2692_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCZ4299333.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            complement(15797..16426)
                     /locus_tag="O2692_001705"
     CDS             complement(15797..16426)
                     /locus_tag="O2692_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HCZ4299334.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
BASE COUNT         5215 a         3134 c         2498 g         5756 t
ORIGIN      
        1 tttttttaaa aaagtaatat atttctatca ataaaagtaa tataatatta gtaattatag
       61 tttcattaat ttattgcaac aatacatagg atcattatta caagaattat ttaatatcat
      121 cttagaatct cacaaaatat caactttgtt taattactca aaatgataag tcatttagag
      181 tttttaaaat aaatttttgc gaaataaagg agacatgtta aatgaaaaaa gtaatgggga
      241 tattattagc aagtacactt atcttaggtg cttgtggaca tcatcaagat agtgcaaaaa
      301 aagagagcac tagtcacaaa aagaaagaaa atgacaatga agaattaaat gaagaactta
      361 aagaatttaa aagcaaaaaa aatatggata taaaaattaa aggcgatact attgttagtg
      421 acaaatttga agctaaaata aaagaaccgt ttatcatcaa tgaaaaagat gagaaaaaga
      481 aatatatcgc ttttaaaatg gaaattactg ctaaaaaaga cgataaagat ttaaatccat
      541 cttctatttc tcatgactat attaatatca ctcaagatga taaaaataca gtaaataaat
      601 taagagatgg ttatctttta agtgataaaa aatataaaga ttggacagaa cataaccaag
      661 atcaaattaa aaaaggcaaa actgcacaag ccatgtttat ctatgagtta agaggtgatg
      721 gaaatattaa tttaaatgtc cataaatact cagaagataa aacagttgat tctaaatcat
      781 tcaaatttag taaacttaaa accgaagatt tttctcatag agcggaaaca agagaagaag
      841 tagaaaagaa agaaaaagaa tttgaagaag agtacaaaaa agaacaagaa cgagagaaag
      901 aaaaagaaaa gcaaaaagat gacgaccaca gtggtttaga tgaagtataa atttggtttg
      961 ttgattgtct tacattcagt tatttaattt ttagagaaac tgacttattt atagcttagc
     1021 caacatgata ctataaagct atgattaaaa ataaaaaatc tgtgtttaat ggtgttaaag
     1081 aaatcagtcc ccgtgccgga aaatcataaa aaaaagatgg gataaatttg taaatcccgc
     1141 attgaataat gcattaaaac aagaagaggc tacatgggga aatgtacaag gtcaagtttc
     1201 acaagcattg atgggaactg gtattaaaga ttctactgca agaagcatag ggttttgggt
     1261 ttcccaagtt ggtcaagcat taatataaaa agagtgtgat aaaatgaata atgaagaatt
     1321 agaaatgaga ttgttattaa tgaaacaatc aatagaacaa ttacaagaag aactagcgcc
     1381 taacttaaaa actagagatt tagtgttatt aagatatatg tattcatata aagaaattaa
     1441 tatgctagat tcttacttgt tccaattggc tacaaataaa gagcaaataa caaaaaaaca
     1501 atttaaaaca aaattggaaa acattagaga agtaccagaa atacctatta gacaagttaa
     1561 tgatatatta gaaggttata aaaatagtga gttatatgtt gagttgataa atagcatcct
     1621 aaaataaaaa taaacatact aagattagct atgaaggaat ctatgacgat agattttttc
     1681 atagctattt tttatagtta taaagaggag tagtctgttc tgactcttgg attttaaata
     1741 tgtataataa acaagtaagc tttactctcg ccttttaaaa ttcgtttgta gtatgttggg
     1801 ttcttaaaat cgtgaatagg aaaatgcaat gagaaaggtt aagtaaagtt tttaacttct
     1861 catttattca atggaaaatt ttttatcgat tacttagaca ttaatattag taaaagaagt
     1921 agtgtagttg caaattataa aaactaaaaa ttccaaaaag agtttatcat ccaaaacaat
     1981 aaaaatggct acaatggtat gttgtgaaat gcatttctta actacgactt cttttatttc
     2041 cgtctcgtca actattgctg attgtgttaa taggcatatt gattctaatg aaaatatcaa
     2101 aactcggctc atctctttat aactacaccc acttagtgac gtaacaaaaa tgaaaagttc
     2161 tttttcagta gccatttatc caattaagtg tagacgattc acatttttat attgacatat
     2221 ataacatgga gaattttttg cgtataaaga agtttcttca tgaacaatgt tgaatgtaaa
     2281 actcagaatc caactttttt gacacttatt tgacacacat cactgtcccc ttatatctca
     2341 aaaactaatt cataatcact tattactctt tcctccatca aataaacacc tatacaaaca
     2401 ctgctatatc aatactcaca acaaattctg caatgcatta tcccctgaaa aacacaaaat
     2461 aaaaaagccc acaaccacaa aggctgtagg ctacaaatat ggagacggcg ggatttgaac
     2521 ccgcgtccaa aggtcctgat acacatcttt ctacgtgtgt agtttatcga taggtttcgc
     2581 atcatgaaaa gtgataaaca accaacatga tgctagtttg attaagtttc ttctaaacag
     2641 acttcaaacg gcagtgttta gcatatccta ttaaggttga atcgcgttaa cagcacatag
     2701 gaaatgctgt taggcgatgc agagtgcgat taggcagcta ctgcgaaatt attgtttgat
     2761 ttgccagtta ttataaactg tgtgtgttga tgacgaagac aaccctccga cacgcttaat
     2821 gagctcgggg gacccctgtc gaatccatga acgtccccga aataataaat caaacaaatc
     2881 atacttttgt aatttacaaa agcacatata actatatcaa atatagcccg attaagcaac
     2941 taaatcgctt aataacgggc tttcatatcg cgcgcaacat ctcgtttgac tgctttttct
     3001 ttcaaagctt gacgtttatc atattttttc ttacctcgtg caacaccaag taatacttta
     3061 caatgtccat gcttcaaata aagctttaac ggcacaatcg aataaccaat ctcacgtgtt
     3121 tgatcaccca atttaatgat ttcacgcttg tgcaataata attttcgaga acgaagagga
     3181 tcgtgattaa aacgattccc ttcttcgtat ggtgctatat gcatattatt caaatacatt
     3241 tcaccgtttt taacttgcgc ataactatct ttaaggttag cactacctcg gcgaattgat
     3301 tttatttctg tgccttgcaa tacaattccc gcttcaatcg tatcttcaat attataatca
     3361 tgtcttgcct tacgattttc cgctaatgta cctggtgatt tcttcttagc cattcatact
     3421 cacctcattg ctgcttattt tttcttacga cgtgctttct ttttcacact tttatcttta
     3481 taaaatggct tatgcttact attacctgat ttatcattat tacgttggtt tttaccttta
     3541 cgttgcttac cttttttctt acgaccctta tcatcagatt ttgatttatc taatgattta
     3601 ccacgcgttt tggcttgaat tgtcttacct cgcgctgggc gctgtgatcg atcatttttc
     3661 ggtaaaggca tacctacaat ttgaaaatca attaatcgtt catctacatc aacatgcgtc
     3721 actttaacct taactgtgtc accaattcta aatactttag cttgacgctc accaattaat
     3781 gccatttgac gctcttcaaa acggtaataa tcatcagtca tattcgcaat atgaaccata
     3841 ccttctatcg tatttggcaa ttcaatgaac ataccgaagt tagctactga gctgacaata
     3901 ccttcaaatt catcaccaat atgttgaatc atatattctg cttttttcaa ttcatcagta
     3961 tcacgttctg cctcaatagc acgacgttca cgtttagaag tatgttcagc taactcaggc
     4021 aatttgtctt cccaacgctt cacttctttg ttatccattg atttctcaat taaatactta
     4081 cggattaaac gatgaactgt taaatcagga taacgtctaa ttggtgatgt aaaatgcgta
     4141 taatattcag ctgataagcc aaaatgtccc aagttcacat catcataatg cgcttgttgc
     4201 attgaacgca acatcattgt tgaaatgacc atttgttcag gtcgaccttc tacttcttct
     4261 tgaacctttt gaagtgttgt tggatgaata tcttcgccag tacccttaat catgatgcca
     4321 aagtttgtaa taaaatcaaa gaattgtctt aagcgatctg atttaggttg ctcatgcact
     4381 cggtaaataa aaggtacatc taacttacta aaatgttcag caactgtttc atttgcaatt
     4441 aacataaatg attcaattag acgttcaccc tcgccacgtt gtcttaattg aacatctgtt
     4501 ggtataccgt cttcgttaac taatactttt gcttcactaa tatcaaaatc gatttcacca
     4561 cgtcgttttc tcatttgaat caaacgatta gataaatctt gtgctaaatc tagcataggc
     4621 gtaatttcat tatattgttc gcgaatgtta ggatcctttt cagtaataat ctgatttacc
     4681 gcatcatacg tcattcgata atcagaatgt ataacactat caaaaatttc atgtttaaca
     4741 acgcgaccac tagcatcgat ttccatgcga cagcttagag ttaaacgatc aacattagga
     4801 ttcaatgaac aaataccatt acttaatcga tgtggaatca ttggaattac acggtcaaca
     4861 agatatacac ttgtcgctct atcatatgcc tctttatcca atgcagaacc ttctgttaca
     4921 taatagctga catcagcaat acttacagtt aattgcgtat taccgttcgc taacttttta
     4981 acactaattg cgtcatctaa gtctttagca tcagcaccat caattgtgat tgtcaattca
     5041 tcacgtaaat catgacggcc tttaatttca gtattttcaa tatgatcagg tactgcttca
     5101 gcttcttgta acacttcatc aggaaattca atttctatgc catgttgata gataatagat
     5161 aaaatatcta cgccaggatc atttttatgt cctaaaatag cagaaatatg tccttctgga
     5221 ttatctgaac catcagcata cttagtaatt tgtacaagta ccttatgacc atcgactgcg
     5281 cctaaacttt gacctttagg aatgaaaata tcttgcataa tacgtttatc atccggaata
     5341 acaaagccaa aatgtctagc ttcactatac gtaccaacaa cttgagttac agaatgcttc
     5401 tcaatcgact taacttcccc ttcgatttta cctttatgtt caccttttga ttgatggatt
     5461 tctacaataa cagtatctcc atccaaggca cgattaattt tcgtcggggg aataaatata
     5521 tcttccatat cctcatcttc aggtcttaag aatgcaaagc cttttttatt ttgacttaac
     5581 gttcctttta tcaattttga ttgacctcta taactatgct ttttttggta tctgtctgtt
     5641 tttgtacgtt cgattaatcc tgattgttct aactccacaa gcaccttaat taaatctcta
     5701 aacgagtcgg cactgcttaa acctaatgca tcttgaaaat ctgacactga cataggttca
     5761 tattcaggtt gattaataat ctcttctata gattgcttta aattcattat gcccctcctt
     5821 tctaatgtta aaatctcttt ttattctgac cagtctaatg actctaaaaa ttgataaata
     5881 tcttcaaata cttgttcttt ctctttatca atcgtaataa catgtccaga ttcactgtac
     5941 cacttgatat ttttgtcatc agaatctaca tggtcatata tataatttgc ggattgtgga
     6001 tcaatcatat tgtcgttttc tgcttgaatc actaaaatag gatccaacac ttcatcaact
     6061 tgctctttaa tcgtatctaa tgcttcactt agttctttta aagtttcagt tggtttaaaa
     6121 tgatccattt cattatcaat agtctcttga tctttacctt catacttttt aaaattgcgt
     6181 gcatattcta aaaagccttc ataaatggca ccttcagttt tgccacccat aggagcacac
     6241 atcgttacaa taccctttac atctctattt aagcttaatt ttaaagcaaa atccccacct
     6301 aatgatagac cagcaacaac aatttcatca taaccttgtt caacaagata atcataacca
     6361 tctaacgcat ctttaaacca aacgaaagga ctagatttca gtatttcatc tggtggtgcc
     6421 gcgtggcctt catattgcgg tgcatatgat gtataacctt tcttttgtaa aaatcgacct
     6481 aattgacgaa catcagacga attgcctgta aaaccatgta gtaataacac ggcacgttta
     6541 ccttcctcaa aaaagaaagg ttttggtaat tttatctgca taaatctaac gctccctttg
     6601 catacttaac taggctatat attttacata gcttattgtt aaactattac attcttaatt
     6661 ataaataaaa aagcccgaca tttacatcgc cggaccttac ataccaagat aacttatgca
     6721 aatcataagt acaaaaaata atattgataa aataattgtt aatctattta agaataaatc
     6781 gacgccacgt tgtttttgtt taccgaataa ctgctcagca ccaccactga tggcacctga
     6841 aagtccactg cttttacctt cttggagtag tacaacagtt attaatgcaa tacaatcaat
     6901 gattaataat acgattaaaa atgtatgcat aaattgtcct ccgttcctta tatacgacaa
     6961 gtagtatatc atatttttat tgttaaaatc aattttctag cgattaaatg tacttttacg
     7021 tttgatagaa acaacaagca tataaatcgc taatgcaacc gaacctataa taacaattaa
     7081 aattggcaat gttaagtacg caccgtgtgc agcttttatt gcttgaattg tgattgataa
     7141 attaatcaac gtaaacaaac tatttgatac ataaactgca aacataaacc accacacata
     7201 agtatgtcga ttccaaaaag tgatgaagcc tgctagataa gccaaaatat acataatacc
     7261 agtgaatcta aagctgttta aaatgtaatc aatcgcttta tctgaagtgt tttgattcgt
     7321 taaattaagt aatgctttgg cagtcgcatt atccaacgaa acaaagtaat gaataatcaa
     7381 taatattgag aatacgattg atgcagttgc aataaagcgt cctgttttaa tttctttatt
     7441 cgttctatca gccatttgtt tactcctcat atatttaaat acgttctata aaacctttat
     7501 ttaattttac cttttaataa aagtgaatac cctgtcatac aatattcatt tcaacataaa
     7561 tttattatac cagtttaaga atacaagtta aatatgtgtt attagttacc tcatgattat
     7621 tagtctgtta tgattaatac ttcaaaataa aaaacctacc acagtctcaa ctctgtggca
     7681 ggctataaat ttaagcgtga acttatttaa gcgtgaactc aaaatcttta tttaatttat
     7741 accgtcataa taatcctcaa ctaaaattat gtcagcattt gattataaag aaaattattt
     7801 atctaagtta tagaatgatt tgataccgtc atatttagca gtttcaaata attcatcttc
     7861 gatacgtaat aattgattgt atttagcaat acggtcagta cgtgataatg aaccagtttt
     7921 aatttgacca gcgtttgtag caacagcaat atcagcaatt gttgtatctt ctgtttcacc
     7981 tgaacggtga gaaactactg ctgtgtaacc agctttttga gccatttcga ttgcatcaaa
     8041 tgtttcagtt aatgtaccga tttggttaac tttaattaag attgagttac caattccgtt
     8101 ttcaatacct tttgctaaaa tttcagtgtt tgttacgaat aaatcgtcac ctactaattg
     8161 tacacggtca ccgatacgtt ctgtaagttg tttccaacca tcccagtcgt tttcgtccat
     8221 accgtcttca attgtaatga taggatattt gtctactaat tgttctaagt agtcaacttg
     8281 ttctgcagct gtacgttttg caccgtgttc gccttcgaac ttactgtagt catatacacc
     8341 attttcatag aattctgatg atgcacagtc aaatcctaag aatacttctt cacctggttt
     8401 gtaaccagct gcttcgattg cttggataat tgtttcaaca gcatcttcag taccttcaaa
     8461 tttaggagcg aaaccacctt cgtcacctac tgcagtttct aaaccacgtt tgcttaaaat
     8521 tgattttaag ttgtggaaaa tttcagtacc ccaacgtaat gattctttga acgttgtagc
     8581 acctacaggt aaaatcatga attcttggaa tgcaattgga gcatctgagt gagaaccacc
     8641 attaacgatg ttcatcattg gtactggtaa ctgcttacca ttaaatccac ctaaatattt
     8701 gtaaagtggt tgacctaata agtcagctgc tgcacgtgct actgcgatag atacacctaa
     8761 aatagcattt gcacctaatt tacctttgtt tggagtaccg tctaatgcga tcatcatttt
     8821 atcaatagat acttgatcta atactgaaaa ttcaccttca ataatttctg gtgcgatgat
     8881 ttcattaacg ttttcaactg ctttagtaac acctttacct aaataacgtg atttgtctcc
     8941 atcacgtaat tcaacagctt cgtgttcacc agttgaagca cctgatggta ctaatgcacg
     9001 accaaatgcg ccactttcag ttaatacttc tacttcaaca gttgggttac cacgagagtc
     9061 taagacttcg cgagcgtaaa catctgtaat aattggcatg tttataatct cctttaaata
     9121 gttattctat aattattata gctttattct ttcatttaac aaaatgatat tactattatt
     9181 ttcaaaaaat aagtgtcatt aagcgtttac ttaacatctt ttacaatatt agtgtttaat
     9241 taaagattca cctgtcatat cttcaggttg ttctacattt aataaatcta ataatgtagg
     9301 tgctaagtca cctaagcgac cagtttctct aagtgtaacg ccttcttttg ttacaatcac
     9361 tggtactggg ttcgttgtat gcgtagtcat tggttgatca tcatccgtca atacttgatc
     9421 agagttacca tggtcagcag taataattgc ataaccgtcc atgtctaaaa tcttatcaac
     9481 cacttctcct aaacattcat caaccgcttc gattgctttg attgtcggct caagcatacc
     9541 actatgtcca accatatcag ggttagcaaa gtttaaaata attaagtcca agtcaccttt
     9601 atttaactct tctaataatg catctttaac ttcataagca ctcatttctg gtttcaagtc
     9661 atacgttgca actttaggtg aatcaattaa acgacggcgt tcacctttaa attcctcgtt
     9721 acgtccacca ctcataaagt aagtaacgtg agggtatttt tcagtttctg caatacgtaa
     9781 ttgagttaaa ttgttatttt gtgcaatttc accaattgta ttatttaaat caactttttc
     9841 gaagacgata gccgcatcga tattgtcatt atacttagtg aatgttgcat agaataagtc
     9901 tttaacttgt tcaactttaa agccttcgaa tgctctgttc gcaaaaattt ccgataattg
     9961 cgctgctcta tcaggtcgga aattatagaa gatcactgca tctccatcat taacaccgtc
    10021 attttgattc tcaacgatga atggtactac gaattcgtca gttaagccct cattatagct
    10081 tgcttctaca ccttctttgg cagttgcata agttggggca tcaaaattac gaatagcatt
    10141 gtaagctttt tcttcacgtt cccaacgttt gtcacgatcc attgcataat aacgaccaga
    10201 cacagatgca aattgaccaa tgcctaattc attgaattta gcttcagtct cttcgatgta
    10261 tttcaaagcg gatttttgat ctacgtcacg gccatctaaa aatgcgtgta cgtaaacttt
    10321 ttcaacacct tgttttttag caagttctaa caaagcaaat aaatgtttgt aatgactgtg
    10381 tacaccaccg tcagacaata aaccaaagat gtgtaacgct gaatcatgtg aattcacgtg
    10441 tgcaattgca ttatttaaaa catcattttc aaagaaatca ccgtcttcaa ttgatttatt
    10501 gattcgagtt aaactttgat aaacgatacg tcctgcaccg atattcatat gaccaacttc
    10561 tgagttaccc atttgtcctt caggtagtcc aacatctaag ccactcgctt cgatttgagt
    10621 cgttggatat ttgttgtaat aacgatcaaa attaggcttg tttgctaatt ttaccgcatt
    10681 accatgttcg ctttcgcggt tcgcaaaacc atctaaaata attaacgcag ttggtttctt
    10741 agccatgatt attttgcacc ttctaacaat tgtacgaaat cttcaacttt aagtgatgcg
    10801 ccacctacta atgccccatc aatatcagtt tgtgccatgt attctttaat gttgttaggt
    10861 ttaacactac caccatattg aatacgagtt gcttctgata cttctttgct tgataagtca
    10921 gcaatagttt gacgtacaaa tgcacacatt tcatttgcat cttcagatgt tgatgattta
    10981 ccagttccga ttgcccagat tggttcataa gcaattacaa ctgatttaag ttgatcttca
    11041 gataaacctg caacagcttt cttaacttgc tcacctacaa catcgttagc tttaccactt
    11101 tcacgctctt cgtctgtttc accaacacat ataattggag tcattccatg tttgaaaata
    11161 gcgtgcgctt ttttgttaat ttcttcatct gtttcgtgga ataattcacg acgttcagaa
    11221 tgaccgataa caacgtattt aacgcctaaa tctgctaatg caactggaga cgtttcacct
    11281 gtgaacgcac cattatcttc gaaatacgta ttttgagcac cgatttctaa accttgtgct
    11341 tttccttctt taactgcagt agttaatgca tctaattgaa ttgctggtgc acaaattact
    11401 gattctactt cttttgaatc tggtaatgtt ggtaatgcat tgacgaagtc ttttgcttct
    11461 tgtactgttt tgttcatttt ccagttacca gctataattg gtgttctcat taaagacact
    11521 ccttgttttg taaatatttt tgaaaagtga tgaaacacga tgtcatcttg tgactgtttt
    11581 cccgtaacaa tgttaaacaa acatgccaca tcactttaaa ctatcacttt attatttatt
    11641 attgattgct ttgataccag gcaattcttt accttctagg tactctaatg acgcgccgcc
    11701 accagttgaa atatgagtga agtcattttc aaaacctaaa gagattgctg ctgcagctga
    11761 atcaccgcca ccgataatcg taattgcatc tttaaggttt gcaattgctt tacatacacc
    11821 aattgtacct tgtgcaaagt tactgaactc gaatacaccc ataggtccat tccatacaac
    11881 agtgtgcgca ccttctaatt catctgcaaa taattttaca gtgtttggtc caatatccat
    11941 accttcttgg tctgctggaa ttgaatcaga tggtactaca gtgattttgg catcattaga
    12001 aaattcttta gcaactttag tgtctactgg taatacaatt ttatcaccat gtttttctaa
    12061 taaatctttt gcgaagtcga ttttatcttc ttctaataat gaaataccaa tttctttacc
    12121 ttgcgctttt aagaaagtat aagccatacc tccgccgatg ataattttat cagctatgtt
    12181 aactaagttt ttgatgacat taattttgtc agatactttt gctccaccta aaatagcaac
    12241 aactggttta tgtggatcgt taactacgcc gccaataaac ttaatttctt tatccattaa
    12301 gaatccagct gcagtttcta aatgtgtaga aataccaaca ttagatgcat gctcacgatg
    12361 cgcagtacca aaagcatcat ttacaaacac atcacctaaa gatgcccagt atttacctaa
    12421 ttctggatca tttttagatt cttttttacc gtctaaatct tcataacgtg tattttcaac
    12481 taataatacg tcgccttctt taaggtcttt aatagcagct tcaagttttt cgccgcgtgt
    12541 ttctggtacg aaaacaactt ctttatctaa tttcttagat aagtcttcag caactggacg
    12601 taaagttaat tttgctttat cactttcttc tttcacttta cctaaatgtg aaaatagtac
    12661 gattttacca ccttgttcga tgatgtattg aattgtaggt aaagcttgaa cgatacggtt
    12721 gtcattagta atttcaccgt cttttaaagg tacgttaaaa tcagcacgta ctaggactgt
    12781 tttaccttta agatctaaat cagaaacaat ttttttagcc atggtgtttc ctccttgtaa
    12841 ttaggaaatt ttatataaaa ataagcggag aagcgtttgt gctccccgct tactcataag
    12901 ctttaattaa tgctaaatag ttctgagcac tagcgtattt gaataaaaac tatactaaaa
    12961 ttatttagaa agttcagcta agtatgctaa tgtacgaact aattgtgcag tatatgacat
    13021 ttcgttatca taccaagctg caactttaac taattgacgg tcgccaactg acattacacg
    13081 agtttgtgta gcgtcgaata atgaaccgta agtcatacct acaacgtctg aagaaacgat
    13141 ttcgtcttca gtgtaaccga atgattcgtt tgaagcattt ttcatagctt cgttaacttg
    13201 ttcaactgtt acgtcttgtt tttctaatac tactgttaat tcagttaatg aacctgtagc
    13261 tacaggaaca cgttgtgcac caccatctaa tttaccatcg atttcaggaa taactttacc
    13321 gatagcttta gcagcacctg ttgagttagg gatgatgttt tctgccgctg cacgagcacg
    13381 acgtttgtca ccttttctgt gaggtgcgtc ttgtgtattt tgatcacctg tgtaagcgtg
    13441 aattgtagtc attaaacctt caactaaacc aaagtcatcg tttaaaactt tagcaactgg
    13501 tgctaatgag tttgtagtac atgaagcacc tgaaacaact gtttcagaac cgtctaactc
    13561 ttggtggtta gtgttgaata cgattgtttt taagtcacca gtagctggtg ctgagattaa
    13621 tactttttta gcgcctgctt caatatgagc ttgtgcttta tctttatcag tgtagaaacc
    13681 agtacattct aatactacat cgatatttaa gtctttccaa ggtaatttgc ttgcatctgg
    13741 ttcactgaat gatttaactt ctttaccatt tacgcggaaa ccaccatcaa ctacctctac
    13801 ttcacctgtg aaacgacctt gcatagtgtc atattttaat aaatgcgcta acatgtcgtc
    13861 atctgttaag tcgtttactg ctacaacttc aagaccttct acttcttgaa ttcttctgaa
    13921 tgctaaacga ccaattctac caaaaccatt aattgctact tttactgcca ttataatggc
    13981 ctcctttaaa atgatattta aaaagtatta aactttttat ctcttattca agtattatct
    14041 ttgctgcggc ttcatcagtg attaacactg tattcttggg tgcaatcgtc aagtatgctt
    14101 taattgcttc acctttcgat ttgcctcctg caactgcaaa aataaagtct tttgattcaa
    14161 ggtcttctaa ttgaagtcca attgttttaa ccttatggac aatttgacct tgtgtatcaa
    14221 aataataacc aaatgcctct ccgacagctt gatgatgttg aagttgttca atgacctttt
    14281 caggtgattg acgtcgatgc gccatcttca gcgcatcacc aatgccgtgt aatataacgt
    14341 ttgcttgttt aattttgtct aaagtgttta tgactgatgg ctctaacaac aatgtattat
    14401 atgttgtttc actgacatta tcaggtacat acatcgtcgt ataataaccg ccagcttgtt
    14461 gtgccatact ggctgcaatt gtgtttgcct gaaagacaac attttcgcct agtccacctc
    14521 tggctggtac gaagaataca ttatatggta ataaatgaat tgcttcacta acacatgcca
    14581 tcgtggatcc gccagttaca gcaactatcg cgtcttcttg taatatgcct tcaagtaatt
    14641 gacctgcttg tctacctaat tctgttttaa cagattgact attatcagca tcaccaggaa
    14701 caacatgaac ttccttaatt tgaaatttat tcttaatacc ttctgacaga cgattatcat
    14761 ctgcatagat atcaaagtaa cctttcaatt gcgaaatcag ttgctcacct tcagctgtaa
    14821 tttccattcc ggtaggctta acttttatca aatcttgttt cttaagcata tctgtttcag
    14881 aacgcagtac acgttcagtc atatccatat gttcgcttaa acttcgacgt ccgacaggct
    14941 gattttttga gatagtagta agaatagaaa aacgtttata cattttatct atgagatccg
    15001 gtataagctt ttgctgtgct tgcaataagt ctttcactac tacctcctcc ttatatttat
    15061 aaatgtaaat aaggatatgt cacaaccagt ggttgccttt ttaagtcccg cgtgggacaa
    15121 aaattaacct ctttacactt gcaatagtac tattctattt caaaaattgc aagcataaaa
    15181 cttaaatttt ctaaataatt tgataaagcg cttgcaatat attttcgatt tgttattcaa
    15241 caaaacgttt tattttattc gctgtagtca aatgtatacc cttatactga ctcgataatc
    15301 atttgcttat tatttgagac taggacataa atcaatgctc tagaatccaa aaagtcacat
    15361 tagtagtagt taaccgaacg aaaatgcact tgtaacaagc ttttttcaat taaagtcagg
    15421 ggccccaaca tagagaattt cgaaatgaaa ttctacaggc aatgcgagtt ggggtggggc
    15481 cccaacatag tgaaattggc tcctcccaat ttctacaggc aatgcaagtt ggggtggggc
    15541 cccaacatag agaaattgga tttccaattt ctacaggcaa tggaagttgg ggtgggacga
    15601 cgaaataaat tttgcgaaaa tatcatttct gtcccacctc actcaaaata aatttcacta
    15661 tccgtaaaat aaataactaa ctatttaagt atcatctctc ccctaattaa caattataaa
    15721 aataagaacc aatattcgag acaacctaat taattgtgtt gtctgtcata ctggttcatt
    15781 tttgtttgac taaatactac ttatcattag cattattttg tttatttgtt gttgattggc
    15841 tattttgttg tggtgcttgt ggttttgctt gctgatttga ttgattgttt tgttgattat
    15901 tatttggctt tggtgttggt tgttgcggct gtggtttagg ctgagctggt tgtttattat
    15961 catcagtttt cttctgatta tcatctgatt tatcttgaga tgctttgtca tccttatctt
    16021 tcgactcatc ctttgatgct ttatcatcat tctcttcttt agcaggacgt tgctttgtag
    16081 gtgcttgttg ctgaggtggc acatattgat attgattctg ctgtggcaat tgctgatatt
    16141 ggtttgcttg tgaatctagc tcattctgct ctttttcctc ttgttgcttt ttctttctct
    16201 ctttttctgc attttctttt tgacgctttt ctttttgttc ctgcttttgt tcgttcaaca
    16261 tacgttcttt agctttattc gaatgatcta catatgcaaa aattgcaaat actaatcctc
    16321 cgagtaatag caataccgtc gcaatgctaa ctactttcgc tgttgtactc attttcttct
    16381 tacttccatc ttcattttta ttgtagtcgt aattttgatt cgacatactc tccctcactt
    16441 caaactaatt taaatataga aattatcacg caaccattaa cattttcaca actaatttgc
    16501 ttacctagtt tctacaaata ttaataaaca tttactcttt ttgccttctt caacttaaaa
    16561 ctgccttcac tattactcac tcgtaaatcg gattcatttt tac
//
LOCUS       DALAGF010000059        16459 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.059, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000059 DALAGF010000000
VERSION     DALAGF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16459
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.059"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            115..768
                     /locus_tag="O2692_001706"
     CDS             115..768
                     /locus_tag="O2692_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCZ4299335.1"
                     /translation="MELKVDDFVKNIKRPYLTVLGVFVVAVSLFFDAIMFFYAKLYDK
                     LPMYLLVFMAFTAVILIMMYIQEKNENYKVEKRYVVRYLTLNVIVGYTLPLLFVSIYV
                     FGVVGFGFDVFNYCLGIILMLFISWLGLFLFYKNEFDSENPNPAVNAIAIIIKLFAFG
                     GIFYISLIVPVTQQEEIFIGLSIFINIIVDALLVRSYFNYALYKSIKKDIENEGKTM"
     gene            802..1389
                     /locus_tag="O2692_001707"
     CDS             802..1389
                     /locus_tag="O2692_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCZ4299336.1"
                     /translation="MIIFILITIFAIYYIAMIASLFKSEGFSIIGLILDVVIMGTLIF
                     YYFIGARFVDHDLSNFLMFMDTGSYIFMYFAIKCLWVKPKVVNYLIAKELGESKEVIE
                     EQELDLQTSKIRGIYFFIISVVMLIITKLRMQPELQADAISMNPVFIFVGVIIILIWL
                     VLDIYRKKKYGIFLFKTIVPLVVTTWIIIATIILS"
     gene            complement(1532..2425)
                     /locus_tag="O2692_001708"
     CDS             complement(1532..2425)
                     /locus_tag="O2692_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ4299337.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
     gene            2673..2966
                     /gene="esxA"
                     /locus_tag="O2692_001709"
     CDS             2673..2966
                     /gene="esxA"
                     /locus_tag="O2692_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HCZ4299338.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            3050..6079
                     /gene="esaA"
                     /locus_tag="O2692_001710"
     CDS             3050..6079
                     /gene="esaA"
                     /locus_tag="O2692_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HCZ4299339.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKSIADSVSGQLNQV
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLVLVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            6079..6537
                     /gene="essA"
                     /locus_tag="O2692_001711"
     CDS             6079..6537
                     /gene="essA"
                     /locus_tag="O2692_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HCZ4299340.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            6509..6751
                     /gene="esaB"
                     /locus_tag="O2692_001712"
     CDS             6509..6751
                     /gene="esaB"
                     /locus_tag="O2692_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HCZ4299341.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            6764..8098
                     /gene="essB"
                     /locus_tag="O2692_001713"
     CDS             6764..8098
                     /gene="essB"
                     /locus_tag="O2692_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HCZ4299342.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            8120..12559
                     /gene="essC"
                     /locus_tag="O2692_001714"
     CDS             8120..12559
                     /gene="essC"
                     /locus_tag="O2692_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HCZ4299343.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGIATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPHVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPNDIPMMPDEIKYEDYRES
                     LNLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHQVANFAEEREDIKAIHQLMIEDLKQREMDGPFEKDSLYII
                     NDFKTFIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            12589..12981
                     /gene="esaC"
                     /locus_tag="O2692_001715"
     CDS             12589..12981
                     /gene="esaC"
                     /locus_tag="O2692_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HCZ4299344.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSFALDDIESTAKDVQTAKSSANKDSVTVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKEIAGEKASEYFNKAMN"
     gene            12997..13311
                     /gene="esxB"
                     /locus_tag="O2692_001716"
     CDS             12997..13311
                     /gene="esxB"
                     /locus_tag="O2692_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HCZ4299345.1"
                     /translation="MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPMADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            13311..13985
                     /locus_tag="O2692_001717"
     CDS             13311..13985
                     /locus_tag="O2692_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HCZ4299346.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLFIRYPLKDKVLNVHIGVINTFIRELFGFDTDENH
                     INKAEE"
     gene            13985..14302
                     /locus_tag="O2692_001718"
     CDS             13985..14302
                     /locus_tag="O2692_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299347.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKAAHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            14312..16156
                     /locus_tag="O2692_001719"
     CDS             14312..16156
                     /locus_tag="O2692_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease YeeF family protein"
                     /protein_id="HCZ4299348.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEELEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNIKRAVTPQNHVTHGPKDSMVRSEGKHSISSHEMNSSKYVES
                     PNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGDRN
                     SHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRPFKEKGHWYNLEKE
                     WQEFLNSGKEVKNIKMEVKYSGNSQRPTIFKVEYEINGERNIRRILNK"
     gene            16167..>16459
                     /locus_tag="O2692_001720"
     CDS             16167..>16459
                     /locus_tag="O2692_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ4299349.1"
                     /translation="MTFEEKLSKIYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPGSDDLNYYTNIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEDLEPWT"
BASE COUNT         6197 a         2240 c         3112 g         4910 t
ORIGIN      
        1 tacataatga ttcttgaaaa atgttggtta acttaataat atgcattttt ttggcgaaga
       61 agatttattt aacttataaa aatattgaag taagattggg gagattatga atttatggaa
      121 ttgaaagtcg atgattttgt aaagaatata aaaagaccat acttgactgt attgggagta
      181 tttgtagttg cagtttcatt attttttgac gcgattatgt ttttctacgc taaactatat
      241 gataaattgc ctatgtattt actggtgttt atggccttta cagctgtaat tttgattatg
      301 atgtacatac aagagaaaaa tgaaaattac aaagttgaaa aaagatatgt ggttagatat
      361 ctcacactta acgttattgt gggatatact ttgccattgc tttttgtatc tatttacgtt
      421 tttggtgtag tcggttttgg atttgatgtt ttcaattatt gtctaggtat tatcttgatg
      481 ttatttattt cttggttagg tttattttta ttttataaaa acgaatttga tagtgaaaat
      541 cctaatcctg cagttaatgc catagcaatt attataaaat tatttgcttt tggaggaata
      601 ttttatataa gtctaatagt tcctgtaaca cagcaagaag agatttttat agggttaagt
      661 atattcatca atataattgt tgatgctctt cttgttaggt catattttaa ttacgcgtta
      721 tataagagta ttaagaaaga tatcgaaaat gaaggtaaga caatgtaaag gaaagacata
      781 aaaaataata ggagtctaac catgataatt tttattttaa taacaatatt tgctatttat
      841 tatatagcta tgattgctag tttgtttaaa agtgaaggtt tttcaataat aggtttaata
      901 ttagatgttg ttataatggg gacattgatt ttttactatt ttataggcgc tcgctttgtt
      961 gatcatgatt taagcaactt tttaatgttt atggatactg gatcatatat ttttatgtac
     1021 tttgcaatta agtgtttatg ggtgaaacct aaggtagtaa attatttgat tgcaaaagaa
     1081 ttgggtgaat ctaaagaggt cattgaagag caggaattag atttacagac atcaaagata
     1141 agaggtatct actttttcat tatttcagta gtgatgttaa ttatcactaa attaagaatg
     1201 caacctgagt tacaggcaga tgctatatcg atgaatcctg tatttatttt tgttggtgtt
     1261 ataatcattt taatttggct agtactggat atatatcgca agaaaaaata cggtatattc
     1321 ttattcaaga cgatagtgcc actagttgtt acgacttgga ttattatagc tacaattata
     1381 ctttcgtaaa cgattttgat taatggggaa tgtagagaac atttctttgt aattttataa
     1441 tcgaataaca tacaaaataa agcgaccaat tttgcagtgt tacgaattgt aaactgcgaa
     1501 ctggtcgctt tttattgatg tcatgattaa tttaatggat gtaattatat gatgaaactt
     1561 ctgaagcaga gatggttctt gatgaaacga tatattcacc aatccagttc atttctgaaa
     1621 ttagaatact tccatcaata ttaacttttt caacgtaggc tacatgacca aatggaccat
     1681 ttactgtttg taaaattgat cctcgtgttg ggtgtctatc tactttgaag ccattgcttg
     1741 aagcttggcc tgcccagttt ttagcatctc cccaaaatgt actaatcgtg tgtccatctt
     1801 tggcacgttt atcaaagaca taccatgtac attgtccagc agtatataag ttgttcttac
     1861 ttgtgataag aggctgatca atgattttac cgttacctaa tgctaaaggt ttaccgtcag
     1921 cggtctttgc tttgtcatta aattcggcga tttgtaattc gtcatacaat tcgtctaaat
     1981 cgatgcctgt aataagccct ttgttatctt ttgaaaaagc gttatttaat tcatcgtcat
     2041 tgtcttcgac attcggtatt gctggtgtca aaggattgct tggtgacgtt tgaggcggtg
     2101 tgtgtgaatc aattgcgtca ttaatgtgcg tatactgacc acttaatgaa gaatggtact
     2161 gattgttgtt aagatcacgt tgatttgcgt gttgatgatt gtcgtttgta cgtgactggt
     2221 tttgatgatt gttgtttggc gtgttgtttt tgtcatatgt ataagtatac gcgccggtgt
     2281 ctttattcac tttgaactgt gcgtttgggt gtgctttctt tgcttcttct aatgttttgc
     2341 tatcattcgt atatgcttga gccgagttag gcgacatact aaataaagta agagttgtca
     2401 tcgtcagtaa aattgttttc ttcataataa ccatttaatc ctttatgtat ttaatttaat
     2461 tttagtatac acatttatat tacaaaaatg aatggttaat taaaaatata tgggtatatt
     2521 caatatattt atttaaaaaa agctaaaaat acttaaaaaa ctatatacat atactaataa
     2581 tttatatatt atttgagtaa ggagcacttt ttcaaaaaat agtgtcccta aaaagttttg
     2641 ataaacttaa aatattcagg aggtttctag ttatggcaat gattaagatg agtccagagg
     2701 aaatcagagc aaaatcgcaa tcttacgggc aaggttcaga ccaaatccgt caaattttat
     2761 ctgatttaac acgtgcacaa ggtgaaattg cagcgaactg ggaaggtcaa gctttcagcc
     2821 gtttcgaaga gcaattccaa caacttagtc ctaaagtaga aaaatttgca caattattag
     2881 aagaaattaa acaacaattg aatagcactg ctgatgccgt tcaagaacaa gaccaacaac
     2941 tttctaataa tttcggtttg caataagcat tctgaaattg gcaaagtcac attttctaat
     3001 gtggctttgc ttatcatttt tttaagaaaa caactgaaag gaaataagca tgaaaaagaa
     3061 aaattggatt tatgcattaa ttgtcacttt aattattata attgccatag ttagtatgat
     3121 attttttgtt caaacaaaat atggagatca atcagaaaaa ggatcccaaa gtgtaagtaa
     3181 taaaaataat aaaatacata tcgcaattgt taacgaggat caaccaacga catataacgg
     3241 taaaaaagtt gagctgggtc aagcatttat taaaaggtta gcaaatgaga aaaactataa
     3301 atttgaaaca gtaacaagaa acgttgctga gtctggtttg aaaaatggtg gataccaagt
     3361 catgattgtt atcccagaaa acttttcaaa attggcaatg caattagacg ctaaaacacc
     3421 atcgaaaata tcgctacagt ataaaacagc tgtaggacaa aaagaagaag tagctaaaaa
     3481 cacagaaaaa gttgtaagta atgtacttaa cgactttaac aaaaacttag tcgaaattta
     3541 tttaacaagc atcattgata atttacataa tgcacaaaaa aatgttggcg ctattatgac
     3601 gcgtgaacat ggtgtgaata gtaaattctc gaattactta ttaaatccaa ttaacgactt
     3661 cccggaatta tttacagata cgcttgtaaa ttcaatttct gcaaacaaag acattacaaa
     3721 atggttccaa acatacaata aatcattatt gagtgcgaat tcagatacgt tcagagtgaa
     3781 cacagattat aatgtttcga ctttaattga aaaacaaaat tcattatttg acgagcacaa
     3841 tacagcgatg gataaaatgt tacaagatta taaatcgcaa aaagatagcg tggaacttga
     3901 taactatatc aatgcattaa aacagatgga cagccaaatt gatcaacaat caagtatgca
     3961 agatacaggt aaagaagaat ataaacaaac tgttaaagaa aacttagata aattaagaga
     4021 aatcattcaa tcacaagagt caccattttc aaaaggtatg attgaagact atcgtaagca
     4081 attaacagaa tcactgcaag atgagcttgc aaataacaaa gacttacaag atgcgctaaa
     4141 tagcattaaa atgaacaatg ctcaattcgc tgaaaactta gagaaacaac ttcatgatga
     4201 tattgtcaaa gaacctgata cagatacaac atttatctat aacatgtcta aacaagactt
     4261 tatagctgca ggtttaaatg aggatgaagc taataaatac gaagcaattg tcaaagaagc
     4321 aaaacgttat aaaaatgaat ataatttgaa aaaaccgtta gcagaacaca ttaatttaac
     4381 agattacgat aaccaagttg cgcaagacac aagtagtttg attaatgatg gtgtcaaagt
     4441 gcaacgtact gaaacgatta aaagtaatga tattaatcaa ttaactgttg caacagatcc
     4501 tcattttaat tttgaaggcg acattaaaat taatggtaaa aaatatgaca ttaaggatca
     4561 aagtgttcaa ctcgatacat ctaacaagga atataaagtt gaagtcaatg gcgttgctaa
     4621 attgaaaaag gatgctgaga aagatttctt aaaagataaa acaatgcatt tacaattgtt
     4681 atttggacaa gcaaatcgtc aagatgaacc aaatgataag aaagcaacga gtgttgtgga
     4741 tgtaacattg aatcataacc ttgatggtcg cttatcgaaa gatgcattaa gccagcaatt
     4801 gagtgcatta tctaggtttg atgcgcatta taaaatgtac acagatacaa aaggcagaga
     4861 agataaacca ttcgacaaca aacgtttaat tgatatgatg gttgaccaag ttatcaatga
     4921 catggaaagt ttcaaagacg ataaagtagc tgtgttacat caaattgatt caatggaaga
     4981 aaactcagac aaactgattg atgacatttt aaataacaaa aagaatacaa caaaaaataa
     5041 agaagatatt tccaagctga ttgatcagtt agaaaacgtt aaaaagactt ttgctgaaga
     5101 gccacaagaa ccaaaaattg ataaaggcaa aaatgatgaa tttaatacga tgtcttcaaa
     5161 tttagataaa gaaattagta gaatttctga aaagagtacg caattgctat cagatacaca
     5221 agaatcaaaa tcaattgcag attctgttag tggccaatta aatcaagtcg acaataatgt
     5281 gaataagcta catgcgacag gtcgagcatt aggcgtaaga gctaacgatt tgaatcgtca
     5341 aatggctaaa aacgataaag ataatgagtt gttcgctaaa gaatttaaaa aagtattaca
     5401 aaattctaaa gatggcgaca gacaaaacca agcattaaaa gcatttatga gtaatccggt
     5461 tcaaaagaaa aacttagaaa atgttttagc taataatggt aatacagacg tgatttcacc
     5521 gacattattc gtattattga tgtatttact atcaatgatt acagcatata ttttctatag
     5581 ttatgaacgt gccaaaggac aaatgaattt cattaaagat gattatagta gtaaaaacca
     5641 tctttggaat aatgtcatta cgtcaggtgt tattggtaca actggtttgg tagaagggtt
     5701 aattgtcggt ttaattgcaa tgaataagtt ccatgtatta gctggctata gagcgaaatt
     5761 catcttaatg gtgattttaa ctatgatggt cttcgtactt attaatacgt atttactaag
     5821 acaggtaaaa tctatcggta tgttcttaat gattgctgca ttgggtctat actttgtagc
     5881 tatgaataat ttgaaagcag ctggacaagg tgtgactaat aaaatttcac cattgtctta
     5941 tatcgataac atgttcttca attatttaaa tgcagagcat cctataggct tggtgctagt
     6001 aatattaaca gtacttgtga ttattggctt tgtactgaac atgtttataa aacactttaa
     6061 gaaagagaga ttaatctaat gttgatgaat agcgtgattg ctttaacttt tttaacagca
     6121 tctagcaata atggcggact taatattgat gtgcaacaag aagaggaaaa gcgaatcaat
     6181 aatgatttaa atcaatatga tacaacgcta tttaataaag acagcaaagc ggttaatgat
     6241 gcgattgcta agcagaaaaa agaacgacaa caacaaataa aaaatgatat gtttcaaaat
     6301 caagcgagtc actcgactcg cttgaatgaa actaaaaaag tgttattttc caaatctaac
     6361 ttagaaaaga cttcggagag tgataaaagc ccctatattc aaaacaagca ggagaaaaaa
     6421 atattcccgt acattttgat gtctgtaggg gcttttttga ctttaggatt tgtcattttt
     6481 tcaattcata aagggagacg aacgaaaaat gaatcagcac gtaaaagtaa catttgattt
     6541 tactaattat aattacggca catatgactt agcagtacca gcatatttac cgataaaaaa
     6601 cttaatagct ttagtattgg atagtttgga catttcaata tttgatgtca atacacaaat
     6661 taaagtgatg acgaaaggtc aattacttgt tgaaaatgat cgactcattg attatcaaat
     6721 cgctgatgga gatattttga agttactata ggaggaaaaa tagatggtta aaaatcataa
     6781 ccctaaaaat gaaatgcaag atatgttaac gcctttagat gctgaagaag cagctaaaac
     6841 aaaattacgc ttagatatga gagagattcc taagtcttca attaaaccag aacattttca
     6901 tttaatgtac ttattagaac aacattctcc atattttata gatgctgaat taactgaact
     6961 acgtgacagt ttccaaatac attatgacat taatgacaat catacacctt ttgataatat
     7021 taaatcattt actaaaaatg aaaaattacg ttacttactc aatatcaaaa atttagaaga
     7081 agtaaatcgt acacgctaca catttgtgtt ggcaccagat gaattatttt tcacaagaga
     7141 tggattaccc attgctaaaa caagagggtt acaaaatgtt gttgatccat tacctgtgtc
     7201 agaagctgaa tttttaacaa gatataaagc gctggttatc tgtgcattca atgagaaaca
     7261 atcatttgat gctttagttg aaggaaactt agaactacat aaaggaacgc catttgaaac
     7321 taaagttatt gaagcggcaa cgttagattt actaacggca tttttagatg aacagtatca
     7381 gaaacaagaa caagattata gtcaaaatta tgcatatgta cgcaaagtag gacataccgt
     7441 tttcaaatgg gttgctatcg gtatgacaac gttaagtgtt ttattaattg cattcttagc
     7501 ctttttatat ttttcagtaa tgaagcataa tgagcgcatt gaaaaaggat accaagcatt
     7561 tgtaaaggat gattatacgc aagtactaaa tacgtatgat gatttagatg gtaaaaaatt
     7621 agataaagag gcactttaca tttatgccaa aagttatatc caaacaaata aacaaggttt
     7681 agaaaaagat aagaaagaaa atttacttaa taatgtgaca ccaaattcaa acaaagacta
     7741 cttattatat tggatggaat taggacaagg acatcttgat gaagcgatta atattgccac
     7801 ttatttagat gataacgata ttacaaagtt agcgttgatt aataaattaa atgagattaa
     7861 aaataacgga gatttatcga atgataaacg ttctgaagaa acgaaaaagt ataacgataa
     7921 attgcaagat attttagaca aagaaaaaca agttaaagat gaaaaagcga aatctgaaga
     7981 agagaaagca aaagcgaaag atgagaaatt aaagcaacaa gaagagaacg aaaagaaaca
     8041 aaaagaacaa gcacaaaaag ataaagaaaa acgccaagaa gctgaaagaa aaaaatagta
     8101 taggactgag gcaaagacaa tgcataaatt gattataaaa tataacaaac aattgaagat
     8161 gctcaatttg cgagatggta agacatatac tattagcgaa gacgagcgtg cagatattac
     8221 gttgaaatcg ttaggcgaag tcattcattt agaacaaaat aatcaaggta cttggcaagc
     8281 gaatcatact tctattaata aggtgcttgt tagaaaaggt gaccttgatg acattacatt
     8341 acagctttat acagaagctg attatgcatc atttgcgtat ccttcaattc aagatacgat
     8401 gacaattgga ccaaatgcgt atgatgatat ggttattcaa agcttgatga atgccatcat
     8461 tattaaagat tttcaatcaa tacaagaatc acaatacgta cgcattgtgc acgataaaaa
     8521 tacagatgtg tatattaact atgaactaca agagcaacta acgaacaaag cttacattgg
     8581 tgatcatatt tatgttgaag ggatatggct cgaagtacaa gctgatggtt taaatgtatt
     8641 gagtcagaat acagtggcat cgtcattaat tcgcttaaca caagagatgc cacatgcaca
     8701 ggcagatgat tacaatacgt accatcgttc gccaaggatt attcaccgtg aaccgacgga
     8761 tgatattaag attgaaagac cgccacagcc aatacagaag aacaatacag tgatatggcg
     8821 ttccattata ccgccattag taatgattgc tttaactgtt gtcatctttt tagtgagacc
     8881 aattggtatt tatattttaa tgatgattgg tatgagtaca gtaacgatag tatttggtat
     8941 tacaacgtat ttctctgaaa agaaaaagta taacaaagat gttgaaaaac gagagaaaga
     9001 ttacaaagct tatttggata ataaatctaa agaaattaac aaagcgatta aagcacaacg
     9061 ttttagtttg aattaccatt atccaacggt tgctgaaatt aaagatatcg ttgaaacgaa
     9121 agcaccaaga atatatgaaa aaacatcgca tcatcacgat ttcttacatt ataagttagg
     9181 tattgcgaat gtagaaaagt cattcaaatt agattaccaa gaagaagaat ttaaccaacg
     9241 tcgtgatgaa ctattcgacg atgctaaaga attgtatgaa ttttacacag atgtagaaca
     9301 agcaccatta atcaatgatt taaatcatgg gccaattgca tatattggtg cacgacatct
     9361 cattttagaa gaattggaga aaatgctaat tcaattgtca acattccata gttatcatga
     9421 tttagagttt ctatttgtga cacgtgaaga tgaagttgaa acattgaaat gggcacgttg
     9481 gttgccacat atgacattga gagggcaaaa cattagagga tttgtttaca atcaacgaac
     9541 gcgtgaccaa attttaacgt caatttatag catgattaaa gaacgtatcc aagctgtgcg
     9601 tgaacgcagc agaagtaatg agcaaattat tttcacaccg caattagtgt ttgtcattac
     9661 agatatgtca ttaattattg atcatgtcat tttagaatat gtaaaccaag atttatcaga
     9721 atatggtatt tcattaatct ttgttgaaga tgtgattgaa agtttgccag agcatgtaga
     9781 taccattatt gatatcaagt ctcgtactga aggcgaactg attacgaaag aaaaagaatt
     9841 agttcaattg aaatttacac ctgaaaatat tgataacgtc gataaagaat atatcgcgcg
     9901 acgtttggcg aatttgatac acgtcgaaca tttgaaaaat gcaattcctg atagtattac
     9961 atttttagag atgtataacg tgaaagaagt agatcagctt gatgtggtta atcgatggag
    10021 acaaaacgaa acatacaaaa cgatggcagt acctttaggt gtaagaggta aagatgatat
    10081 tttatcattg aacttacatg aaaaagcaca cgggccacat ggtttagttg ctggtaccac
    10141 tggttcaggg aaatctgaga ttatccaatc atacatttta tctttagcta ttaattttca
    10201 ccctcatgaa gttgcattcc tattgattga ctataaaggt gggggtatgg cgaacttatt
    10261 taaagattta gtccatttag ttggtacgat tacaaactta gatggcgatg aagcgatgcg
    10321 tgccttaaca tcaatcaaag ccgaattgag aaaacgtcaa cgtttattcg gagagcatga
    10381 tgttaaccat attaatcaat accataagtt atttaaagaa ggtattgcga cagaaccaat
    10441 gccacattta ttcattattt ccgatgagtt tgccgaatta aaatcagaac aacctgattt
    10501 tatgaaagaa cttgtatcaa cggcacgtat tggacgttcg ttaggtattc atttaatact
    10561 tgcgacacaa aaaccatcgg gtgttgttga tgaccaaatt tggtctaact ctaaatttaa
    10621 gttggcatta aaagtacaag atagacaaga cagtaatgaa attttaaaaa caccagatgc
    10681 agcagacatt acattaccag gtcgtgcgta tttacaagtt ggtaataatg aaatttatga
    10741 attattccaa tctgcatgga gtggtgcaac atatgacatc gaaggcgata aattagaagt
    10801 tgaagataag acgatttaca tgattaatga ctatggtcaa cttcaagcaa tcaacaaaga
    10861 cttgagtgga cttgaagatg aagaaacgaa agaaaatcaa actgagttag aagcggtcat
    10921 agatcatatc gaatctatta caacacgatt agaaatcgaa gaagttaagc gtccatggct
    10981 accaccattg ccagaaaatg tatatcaaga agatttagta gaaacagatt tcagaaaatt
    11041 atggtcagat gatgcaaaag aagtggaatt aacattagga cttaaagacg taccagaaga
    11101 acaatatcaa ggaccgatgg tattgcaatt gaaaaaagct gggcacatcg cgttaatcgg
    11161 aagtccagga tatggtagaa caacgttctt acacaacatt attttcgatg ttgcaagaca
    11221 ccatcgtcct gatcaagcac acatgtactt gttcgatttc ggtaccaatg gtttgatgcc
    11281 agttacagac ataccacatg tcgctgatta ctttacagta gatcaagaag acaagattgc
    11341 taaggcgata cgtatattta atgatgaaat tgatcgtcgt aagaagattt taagtcagta
    11401 tcgtgtcact agtatttctg aatatcgaaa attaactggt gaaacaattc cgcatgtctt
    11461 tattcttatt gataactttg acgcagtaaa agattcacct ttccaagaag tttttgaaaa
    11521 tatgatgatt aaaatgacgc gtgaagggct agcattagac atgcaagtaa ccttaactgc
    11581 ttcaagagct aacgctatga aaacaccaat gtacattaat atgaaaacgc gtatcgcaat
    11641 gtttttatat gataaatcag aggtgtcgaa cgtagtagga cagcaaaaat ttgcggttaa
    11701 agatgttgtg ggtcgagcat tgttaagtag tgatgacaac gtatcattcc atattggcca
    11761 accatttaaa catgatgaga ccaaatcata taatgatcaa attaatgatg aagtatcggc
    11821 gatgacagaa ttttataaag gtgaaacacc aaatgatatt cctatgatgc cagatgaaat
    11881 taaatatgaa gattacagag aatcattaaa cttaccagat atagttgcaa atggtgcttt
    11941 accaattgga ttagattatg aaggtgttac actacaaaaa attaaattaa ctgaaccagc
    12001 aatgatttca tcagaaaatc cgagagaaat tgcgcatatt gctgaaatta tgatgaaaga
    12061 aattgacata ttaaatgaaa aatatgcgat ttgtatcgca gactcaagtg gagagtttaa
    12121 agcttatagg catcaagtgg ctaactttgc cgaagaaaga gaagacatta aagcgattca
    12181 tcaactaatg attgaagact taaagcaaag agaaatggac ggcccatttg aaaaagattc
    12241 actttatatt atcaatgatt ttaaaacatt tattgattgc acgtatattc cggaagatga
    12301 tgttaaaaag cttattacaa aaggaccaga acttggcttg aacattttat ttgtcggcat
    12361 tcataaagaa ttaatagatg cttatgataa acagattgat gttgcacgta aaatgattaa
    12421 ccaatttagt ataggtattc gtatttcaga ccaacaattc tttaaattta gatttattca
    12481 acgagaacct gttattaaag aaaatgaagc atatatggtc gcaaaccaag cttatcaaaa
    12541 gattagatgg tttaaatagc aatgaattaa ataggaggga ggtatgttat gaattttaat
    12601 gatattgaaa caatggttaa gtcgaaattt aaagatatta aaaagcatgc tgaagagatt
    12661 gcgcatgaaa ttgaagttcg ttctggatat ttaagaaaag ctgaacaata taagcgatta
    12721 gaatttaatt tgagttttgc actagatgat attgaaagca cagcaaagga cgtacaaact
    12781 gcaaaatcta gtgctaataa ggacagtgta actgttaagg gaaaggcgcc caatacgtta
    12841 tatattgaaa aaagaaattt gatgaaacaa aagcttgaaa tgttgggtga agatatcgat
    12901 aaaaataaag aatccctcca aaaagctaag gaaattgctg gcgaaaaggc aagtgaatat
    12961 tttaataaag caatgaatta atattgaggt gaagatatgg gtggatataa aggtattaaa
    13021 gcagatggtg gcaaggttga tcaagcgaaa caattagcgg caaaaacagc taaagatatt
    13081 gaagcatgtc aaaagcaaac gcaacagctc gctgagtata tcgaaggtag tgattgggaa
    13141 ggacagttcg ccaataaggt gaaagatgtg ttactcatta tggcaaagtt tcaagaagaa
    13201 ttagtacaac cgatggctga ccatcaaaaa gcaattgata acttaagtca aaatctagcg
    13261 aaatacgata cattatcaat taagcaaggg cttgataggg tgaacccatg atgaaagatg
    13321 ttaagcgaat agattatttt tcttacgaag aattaacaat tttaggtggt agtaaattgc
    13381 ctctcgtaaa ttttgaattg tttgatccat caaattttga agaagctaaa gctgctttaa
    13441 ttgaaaagga attagtaaca gagaatgaca agttaactga tgcaggtttt aaagtggcta
    13501 cattagtcag agagtatatt agcgccattg taaatattcg aattaatgat atgtattttg
    13561 caccatttag ctatgaaaaa gatgaatata ttttgttaag ccggtttaaa aataatgggt
    13621 ttcaaatacg aattatcaat aaagacattg catggtggtc gattgtacaa tcatatcctt
    13681 tattgatgag acaagaaaag tccaatgatt gggactttaa acaaattgac gatgaaacat
    13741 tggagaactt aaataatgaa agtatcgata cgattgggcg tgttttagaa attgaaatat
    13801 acaatcatca aggtgaccct caacaaagtt tatataacat ttatgaacaa aatgatttgt
    13861 tattcattcg atacccatta aaagataaag tgctgaatgt tcatattggt gtcattaata
    13921 catttatacg agaattattt ggattcgata ctgatgaaaa tcatattaat aaagcagagg
    13981 agtaatgacg ttgagtggaa aaattagtgt taaagctgaa acgattgcac atgttgtaaa
    14041 agaattggaa agcataagtc aaaagtatga tgaaatagct caaaactttg gaaaaatagc
    14101 gcaattaaat tactacagta gtgaaaaagc tgcacattct atggaaaatg gctatagtag
    14161 tgctgcaaca gtcattagtg gtctcaaagg tccattgagt acactcggtg gtggcgtcat
    14221 gaattcagca caaaagttct ttgaagcaga tgaacattgg ggtacggaat ttgccaagct
    14281 ttactataat attgagggat aggtgcatga catgacaaaa gatattgaat atctaacagc
    14341 tgattatgac aatgaaaagt catctatcca aagtgtaata gatgcaatag agggtcaaga
    14401 cttcttagat gtagatacaa caatggatga tgcggtaagc gatgtcagtt ctttagacga
    14461 agatggcgca atatcattaa caagtagtgt agtaggtcca caaggatcta aattaatggg
    14521 gtattatcaa aatgagttat atgattatgc atctcaatta gattcgaaaa tgaaagaaat
    14581 tattgacacg ccatttatag aagatataga taaagcattc aaaggtataa cgaatgttaa
    14641 attggaaaat atactaatta aaaatggtgg tggacatggt agagatacct atggggcttc
    14701 tgggaaaatt gcaaagggag atgccaagaa aagtgacagc gatgtttata gcatcgatga
    14761 aatattaaaa tcggatcaag aatttgtaaa agtaattgat cagcattaca aagaaatgaa
    14821 aaaagaagat aagaaattat ctaaaagtga ttttgaaaaa atgatgactc agggcgcttc
    14881 ttgtgattac atgacagtag ctgaagcgga agagctagag gagcaaaaga aaaaagaaga
    14941 agctatagag attgcagcac tagctggtat ggtagtttta tcttgtatta atcctgttgc
    15001 tggagcagta gctattggtg cttattccgc ttattcagca gcaaatgcag ccacaggaaa
    15061 aaatattgta actggaagaa agctatctaa agaagaacga atcatggaag gactttcgct
    15121 tattccattg ccaggtatgg gcttcctcaa aggtgctggg aaaagtttaa tgaaattagg
    15181 cttcaaaggc ggagaaaaat ttgcagttaa aacaggattg caaaagacaa tgcaacaagc
    15241 agttagtcgt atttcaccta aaatgggaat gatgaaaaac agtgtgttga atcaatctcg
    15301 taactttgct caaaatactc atgttggaca aatgctgagt aacatgcgtg gtcaagcaac
    15361 tcatactgtt caacaaagta gaaattggat tggacaacaa gcacaaaatg tcaaacgaat
    15421 agtgaataat ggacttgata aagaaatagc acatccattt aaacaacaac ttgcaccagc
    15481 gggaatgggt ggtataaaat ttgctgaaac aactactttg agaaacatgg gtcaaaacat
    15541 aaagcgtgct gttacaccac aaaatcacgt gacacatggt ccaaaagata gtatggtgag
    15601 aagtgaaggc aaacatagta taagtagcca tgaaatgaat tcatcaaagt atgttgaatc
    15661 accaaactac accaaggttg aattcggaga acactatgca agacttagac ctaagaaact
    15721 aaaggcgaat attgaataca caacacctac tggtcacata tatcgaaccg atcataaagg
    15781 tcgcataaaa gaagtttatg tagacaatct ctctctaaaa gatggggatc gtaatagcca
    15841 tgcacaaaga actgtggggg gagaggatag attaccagac gatgatggag gtcacttaat
    15901 cgctagaatg tttggtggtt caaaagacat tgataacctt gtggcacaaa gtaaatttat
    15961 caaccgtcca tttaaggaga aaggtcattg gtataatctt gagaaagagt ggcaagagtt
    16021 cttaaactct gggaaagagg tgaaaaatat taaaatggaa gtaaaatata gcggtaatag
    16081 tcaaagaccg actatattta aagttgaata tgaaattaat ggtgaaagaa atattagaag
    16141 aatattaaat aagtagaggt gccaacatga catttgaaga gaagcttagc aaaatataca
    16201 atgaaattgc gaatgagatt agcagtatga taccggtaga gtgggaaaaa gtatatacaa
    16261 tggcttatat agatgatgga ggaggtgaag tattctttaa ttatactaaa ccaggtagtg
    16321 atgacttgaa ttattacacc aatataccta aggagtataa catttctgtg caagtatttg
    16381 atgatttatg gatggattta tatgatttat ttgaggagtt aagagattta tttaaagaag
    16441 aagatttaga accatggac
//
LOCUS       DALAGF010000060        16458 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.060, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000060 DALAGF010000000
VERSION     DALAGF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16458
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.060"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(179..1045)
                     /locus_tag="O2692_001721"
     CDS             complement(179..1045)
                     /locus_tag="O2692_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCZ4299350.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            complement(1598..1702)
                     /locus_tag="O2692_001722"
     CDS             complement(1598..1702)
                     /locus_tag="O2692_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299351.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYGEMQ"
     gene            1797..3107
                     /gene="sbi"
                     /locus_tag="O2692_001723"
     CDS             1797..3107
                     /gene="sbi"
                     /locus_tag="O2692_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HCZ4299352.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKVESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNTYYKYKGAIDQTVLTVLGSGSKSYIQPLKVDDKNGYLAKSYAQVRNYVT
                     ESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTLSNLLSFWK"
     gene            3643..4572
                     /gene="hlgA"
                     /locus_tag="O2692_001724"
     CDS             3643..4572
                     /gene="hlgA"
                     /locus_tag="O2692_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HCZ4299353.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
     gene            5139..6086
                     /gene="hlgC"
                     /locus_tag="O2692_001725"
     CDS             5139..6086
                     /gene="hlgC"
                     /locus_tag="O2692_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HCZ4299354.1"
                     /translation="MLKNKILTTTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKA
                     MRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKD
                     PRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN"
     gene            6088..7065
                     /gene="hlgB"
                     /locus_tag="O2692_001726"
     CDS             6088..7065
                     /gene="hlgB"
                     /locus_tag="O2692_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HCZ4299355.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(7117..7584)
                     /locus_tag="O2692_001727"
     CDS             complement(7117..7584)
                     /locus_tag="O2692_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCZ4299356.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLAS
                     VLGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLRKRMAVLLR"
     gene            complement(7595..8287)
                     /locus_tag="O2692_001728"
     CDS             complement(7595..8287)
                     /locus_tag="O2692_001728"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HCZ4299357.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFAAAKIGYHRLFDIKPVNTRYGGRIIFVDDCIDLNHYISFL
                     ESTPKQVVYETV"
     gene            complement(8298..9413)
                     /locus_tag="O2692_001729"
     CDS             complement(8298..9413)
                     /locus_tag="O2692_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HCZ4299358.1"
                     /translation="MKFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            complement(9391..10398)
                     /gene="bioB"
                     /locus_tag="O2692_001730"
     CDS             complement(9391..10398)
                     /gene="bioB"
                     /locus_tag="O2692_001730"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCZ4299359.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNEVSRA
                     N"
     gene            complement(10400..11758)
                     /gene="bioA"
                     /locus_tag="O2692_001731"
     CDS             complement(10400..11758)
                     /gene="bioA"
                     /locus_tag="O2692_001731"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCZ4299360.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(11736..12422)
                     /gene="bioD"
                     /locus_tag="O2692_001732"
     CDS             complement(11736..12422)
                     /gene="bioD"
                     /locus_tag="O2692_001732"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HCZ4299361.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQMTIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            complement(12476..12643)
                     /locus_tag="O2692_001733"
     CDS             complement(12476..12643)
                     /locus_tag="O2692_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299362.1"
                     /translation="MQLLILTDIFYRIAVYICPNSLRSLSHFNSKVKILPKFIVILRS
                     LNSCFLEKHAP"
     gene            complement(12874..14607)
                     /locus_tag="O2692_001734"
     CDS             complement(12874..14607)
                     /locus_tag="O2692_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4299363.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            complement(14632..16395)
                     /locus_tag="O2692_001735"
     CDS             complement(14632..16395)
                     /locus_tag="O2692_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4299364.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNTYTINSIMVYIFIALIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATVYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
BASE COUNT         5761 a         2940 c         2225 g         5532 t
ORIGIN      
        1 cccattttca ttttttaatt cataaatgaa tcaataacca cctaataaca aatcatatta
       61 tacacctttg ttcgctattt ttctaaggtt taaaaaatat ttttaggtaa acctaaaaat
      121 agatgtaata aaaacgcctc ctcagatatt tatatatcta tgaagacgtt taaatacatt
      181 atagatggtc tggttctggg tgaacgtata ctgaggaaat accttttttg tgcaaatgat
      241 gttcgacatt gtcacaaatt tgatgcgctt ctactaagga aaggttagca tctacaacaa
      301 ttgtgacatc aataaacaca ctacttccat ggtaacgccc tttaatactt ttaacttctt
      361 gtacttcatc aacttctaaa atatcattgc gatacgcttc taattcagtt tcattgaaac
      421 catcactcaa cataaaaatt gcttctttaa aaataccaaa accagtataa acgattagta
      481 agcctagtaa tgttgctaaa ataatatcga caatcgggaa accgatttgc gtaaaaatta
      541 atcctatcgc tgttccaatg ctgactaaac tatccgataa attatctttg gcagccgaat
      601 ttaaagaact acttttcgtt cttttcgcta gtctttgatt gactgcaaat acaatcaaca
      661 ttacaagacc actgattaag ctgacgataa ttgttattgc gttaggtaca acgtcatctt
      721 ctttgaacaa acgaggtgca ttttgaataa ctacttggat acctacaaac ataatgacaa
      781 atgacaccaa taatgaagaa atattttcag acttcaaatg gccataagga tgatttcgat
      841 cggcaggttt aattgaaatt ttcaatccaa taataacagc taaagaaacg ataatatctg
      901 tcatattgtt taatgcatcg gctcttacag ctgcagagtt aaagacaaaa cccgtgacat
      961 acttaacaat agataagatt atatatacaa ttaaactcaa ataagcaccg cgttgcgcca
     1021 atttaagatt ttcattatga gacatgcgtt gaaccacctt gaattagtat agtaacaata
     1081 ttatgaatga ttcattttaa ttttacaacg tttttaattt ttataaattt ttataaaatt
     1141 aaactaattt attcattcgc aacccctaaa aataattttt agcctttctg cgaattttat
     1201 gagctagaaa ggcgcccaac tctccctgtt tgttaacttt cgcctcgaaa gtttctatgt
     1261 tgggacccta tgcattagta gcgaagctat ctaatgtgtt cctttatttt ctgcgaattt
     1321 tatgagctag aaaggcttat gcagttgacg ttttacgtcc aactgcgttc ctccgtcttc
     1381 ttcaaattta tttgttagaa aggcacccaa ctctccctgt ttgttaactt tcgcctcgaa
     1441 agtttctatg ttagaaccct atgcatgagt tgcgaagcta tctaatgtcg tgaactaatt
     1501 atatagaaga aaaagtgcat caatgacaaa ttaaatgaga tttctactct accaaactct
     1561 cttcgaaaga caattttctc ctctatttat tagcaaacta ttgcatttct ccatatagta
     1621 cttccttact taaaatacgc tgaatgttct gaattaaacg cttttttaca tagttaacac
     1681 tagttaatct attagttaac attagttaat aattagttaa tttccatttg tattctcatg
     1741 tgataaattc taaaagcata caataaattt aatatgtaaa aagaaaggga atacacatga
     1801 aaaataaata tatctcgaag ttgctagttg gggcagcaac aattacgtta gctacaatga
     1861 tttcaaatgg ggaagcaaaa gcgagtgaaa acacgcaaca aacttcaact aagcaccaaa
     1921 caactcaaaa caactacgta acagatcaac aaaaagcttt ttatcaagta ttacatctaa
     1981 aaggtatcac agaagaacaa cgtaaccaat acatcaaaac attacgcgaa cacccagaac
     2041 gtgcacaaga agtattctct gaatcactta aagacagcaa gaacccagac cgacgtgttg
     2101 cacaacaaaa cgctttttac aatgttctta aaaatgataa cttaactgaa caagaaaaaa
     2161 ataattacat tgcacaaatt aaagaaaacc ctgatagaag ccaacaagtt tgggtagaat
     2221 cagtacaatc ttctaaagct aaagaacgtc aaaatattga aaatgcggat aaagcaatta
     2281 aagatttcca agataacaaa gcaccacacg ataaatcagc agcatatgaa gctaactcaa
     2341 aattacctaa agatttacgt gataaaaaca accgctttgt agaaaaagtt tcaattgaaa
     2401 aagcaatcgt tcgtcatgat gagcgtgtga aatcagcaaa tgatgcaatc tcaaaattaa
     2461 atgaaaaaga ttcaattgaa aacagacgtt tagcacaacg tgaagttaac aaagcaccta
     2521 tggatgtaaa agagcattta cagaaacaat tagacgcatt agttgctcaa aaagatgctg
     2581 aaaagaaagt ggcgccaaaa gttgaggctc ctcaaattca atcaccacaa attgaaaaac
     2641 ctaaagtaga atcaccaaaa gttgaagtcc ctcaaattca atcaccaaaa gttgaggttc
     2701 ctcaatctaa attattaggt tactaccaat cattaaaaga ttcatttaac tatggttaca
     2761 agtatttaac agatacttat aaaagctata aagaaaaata tgatacagca aagtactact
     2821 ataatacgta ctataaatac aaaggtgcga ttgatcaaac agtattaaca gtactaggta
     2881 gtggttctaa atcttacatc caaccattga aagttgatga taaaaacggc tacttagcta
     2941 aatcatatgc acaagtaaga aactatgtaa ctgagtcaat caatactggt aaagtattat
     3001 atactttcta ccaaaaccca acattagtaa aaacagctat taaagctcaa gaaactgcat
     3061 catcaatcaa aaatacatta agtaatttat tatcattctg gaaataatca atcaaaaata
     3121 tcttctctag ttttacatca ttttttaaat aattttcgta acaaaccgtg attaaaaaga
     3181 accgttgatt ctcaatcgaa tctacggttc ttttttcatt ttccatcaat taaatgcttc
     3241 ttcgctattt gtcagcccac ttttttacct gcaacttgtt aaataatcct tacatcgtta
     3301 acgaatagtt catcatttag ttgaatcagc tcaactttat taacttcata ttttcacaaa
     3361 ctattgcgca atccattcct tttccactac aagcaccata attaaacaac aattcaataa
     3421 aataagactt gcaaagcata gttatgtagc tatataaacg cctgcgacca ataaatcttt
     3481 taaacataac ataatgcaaa aacatcattt aacaatgcta aaaatgtctc ttcaatacat
     3541 gttgatagta attaactttt aacgaacagt taattcgaaa acgcttacaa atggattatt
     3601 atatatatga acttaaaatt aaatagaaag aaagtgattt ctatgattaa aaataaaata
     3661 ttaacagcaa ctttagcagt tggtttaata gcccctttag ccaatccatt tatagaaatt
     3721 tctaaagcag aaaataagat agaagatatc ggccaaggtg cagaaatcat caaaagaaca
     3781 caagacatta ctagcaaacg attagctata actcaaaaca ttcaatttga ttttgtaaaa
     3841 gataaaaaat ataacaaaga tgccctagtt gttaagatgc aaggcttcat tagctctaga
     3901 acaacatatt cagacttaaa aaaatatcca tatattaaaa gaatgatatg gccatttcaa
     3961 tataatatca gtttgaaaac gaaagactct aatgttgatt taattaatta tcttcctaaa
     4021 aataaaattg attcagcaga tgttagtcag aaattaggct ataatatcgg cggaaacttc
     4081 caatcagcgc catcaatcgg aggcagtggc tcattcaact actctaaaac aattagttat
     4141 aatcaaaaaa actatgttac tgaagtagaa agtcagaact ctaaaggtgt taaatgggga
     4201 gtgaaagcaa attcattcgt tacaccgaat ggtcaagtat ctgcatatga tcaatactta
     4261 tttgcacaag acccaactgg tccagcagca cgagactatt tcgtcccaga taatcaacta
     4321 cctcctttaa ttcaaagtgg ctttaatcca tcatttatta caacattgtc acacgaaaga
     4381 ggtaaaggtg ataaaagcga gtttgaaatc acttacggca gaaacatgga tgctacatat
     4441 gcttacgtga caagacatcg tttagccgtt gatagaaaac atgatgcttt taaaaaccga
     4501 aacgttacag ttaaatatga agtgaactgg aaaacacatg aagtaaaaat taaaagcatc
     4561 acacctaagt aaacagttca atcatcttaa aaaatcctgg gacacttcat acttgtctca
     4621 ggatttttta acaaattgaa tcagccttat aacattaaat tattttatcg tacattaaat
     4681 ttaataataa caactgattt ttataagaat aaagtatcga accatagtag atacacaaat
     4741 aatacaaatg aaacaattta acttgaaagc ttaaataaat attatcaagt taataaacaa
     4801 ttaattttta gatggattca tcaaaaatcg taaaaagcac aatttgtatt ttacaaacat
     4861 taattaaaaa agaaagcaag acattcgtgc aatcggttac cttaaattgt ttacaactgt
     4921 caacaatacc aaggttttat taactatatt tctcacaaaa ttagctttta gcattccaaa
     4981 caaaaaaggt taaatcgaac ggaattatgg catttttaac ttaattgtaa aaaaagttga
     5041 taatggtcaa ttgttaatga acagttaatt ataataacgc ccaaaatata ttattattta
     5101 attaagttaa ataaaattat agaaataaag tgaaacttat gcttaaaaat aaaatattaa
     5161 ctacaacttt atctgtgagc ttacttgccc ctcttgccaa tccgttatta gaaaatgcta
     5221 aagctgctaa cgatactgaa gacatcggta aaggaagcga tatagaaatt atcaaaagga
     5281 cagaagataa aacaagtaat aaatggggcg tgactcaaaa tattcaattt gattttgtaa
     5341 aggataaaaa atataacaaa gatgctttga tattaaagat gcaaggattc attagctcta
     5401 gaacaacata ttacaactat aaaaaaacta atcatgttaa agctatgcga tggccattcc
     5461 aatataatat tggtttaaaa acaaatgata aatatgtttc tttaattaat tatttaccta
     5521 aaaataaaat tgaatctaca aacgtgagtc agacattagg atacaatatc ggtggtaatt
     5581 tccaatcagc cccatcactc ggtggtaatg gatcatttaa ctattctaaa tcgattagct
     5641 atacacaaca aaattatgta agtgaagtag aacaacaaaa ctcaaaaagt gttttatggg
     5701 gcgtcaaagc gaattcattc gccactgaat caggtcaaaa atcagccttt gatagcgatt
     5761 tatttgtagg ctacaaacct catagtaaag atcctagaga ttatttcgtt ccagacagtg
     5821 agttaccacc tcttgtacaa agtggattta acccttcatt tatcgccaca gtatctcatg
     5881 aaaaaggttc aagcgataca agcgaatttg aaattactta cggaagaaac atggatgtca
     5941 ctcatgccat taaaagatca acgcattatg gcaacagtta tttagacgga catagagtcc
     6001 ataatgcatt tgtaaataga aactatactg tgaaatacga ggtcaattgg aagactcatg
     6061 aaatcaaggt gaaaggacag aattgatatg aaaatgaata aattagtcaa atcatccgtt
     6121 gctacatcta tggcattatt attactttct ggtactgcta atgctgaagg taaaataaca
     6181 ccagtcagcg taaaaaaagt cgatgacaaa gttactttat acaaaacaac agccacagca
     6241 gattctgata aatttaaaat ttcacagatt ttaacattta atttcatcaa agataaaagt
     6301 tatgataaag atactttagt acttaaagct actgggaata ttaactcagg ctttgtgaaa
     6361 cctaatccta atgactatga cttttcaaaa ttatattggg gagctaaata caatgtatct
     6421 ataagctcac aatctaatga ttcagtaaac gtcgttgatt atgcaccaaa aaatcaaaat
     6481 gaagagtttc aagttcaaaa tactttaggc tatacatttg gtggtgacat tagtatctct
     6541 aatggtttat ctggtggact taatggaaat acagcttttt ctgaaacaat taattataaa
     6601 caagaaagtt acagaacaac attaagtcgc aacacaaatt ataaaaatgt tggctgggga
     6661 gttgaagcac ataaaattat gaataatggt tggggacctt atggaagaga tagcttccac
     6721 ccaacatatg gtaatgaact cttcttagct ggcagacaaa gcagtgcata cgctggccaa
     6781 aacttcatag cgcaacacca aatgccatta ttatctagaa gtaacttcaa tccagaattt
     6841 ttaagcgtac tatcacacag acaagatggc gctaaaaaat ctaaaattac agtaacttat
     6901 caacgtgaaa tggatttata ccaaattcgt tggaatggct tctactgggc aggcgcaaat
     6961 tataaaaact ttaaaactag aacatttaaa tcaacatatg aaattgattg ggaaaatcac
     7021 aaagtgaaat tgttagatac aaaagaaact gaaaacaata aatagctagt aaaacacggt
     7081 cgccaacagt aattgtgacg accgtgtttt gatttattat cttagtaata ctgccattct
     7141 ttttctcaat gtgagatata aaggaatagc tacaattaaa gtgaatatta cgcctggaat
     7201 cgcgtttaac aacactaccc acacaggtaa atttaaaata atagataata gtagcctaga
     7261 taccaaactg cctaatacac ttgctaaaac taatgatagt acatttattt tcaataaata
     7321 aacaactgca atagctataa ctctaaatat aatagagata atcacattaa ttggattaaa
     7381 tacgccaaat actagtaata ataggctaga taataatcca cctaaaaagt actttttaat
     7441 tccaaagaaa gctaatatca ataatgctgc cggtgcagat aattgaaaat ctaatcctgg
     7501 tataatggac ggtattttca aaactgccaa aatggttaaa atcgcagcaa tgacactaat
     7561 ttgagtaata tcttttgatg tcatactaaa acccctatac cgtttcataa acaacttgct
     7621 tcggtgtgct ttctaaaaat gatatgtaat gatttaaatc aatacaatcg tccacaaata
     7681 ttattctgcc tccatatctc gtattaactg gtttaatatc aaataatcga tggtaaccaa
     7741 ttttagcagc ggcaaaataa cctgtcgtat acgttaagtc atcggacacg caaagttctc
     7801 ctttgacata cggatgcgca ttgatacaac tagcaattgc taaggcatca gtcactcttt
     7861 cattaagatc acctttatta tttatatctt caaacgaaaa atgtgttgcc ctaatccccc
     7921 tttgtccaaa tgaatctaaa cgtttaccag atatagcaga tagaataata gctcctgtat
     7981 aaaccgtttc atttttaata tatgtcatcc cttgatttag cgcttgttca gtgacaccac
     8041 attcttgtgt taaatgttgg agatttgctt tatcatcctc aataatttgt aatgctttta
     8101 tttgttgaat cggttccatg attttttgta tttttatatt gagaaaatcc ggttgcccat
     8161 tttcatgaaa aaaaccttta ttataaaacc tttgtaccgt ctgttctatt ttgtcaaact
     8221 cacaaatcgt ttcagcacca ctaatatgaa catcttgatt gctagaacgc atttttatac
     8281 tatacatgac gatcacctca atcttcttga tgcaaaattt caaacaacct atctatatct
     8341 tgttcagtat gaaaatacga caatgatatt cttaacattg gcttagtcac agttggatac
     8401 cttaaataac ttgtaaacac atgatgcttt aataatgttt gatgaatgtt ctcagccgct
     8461 tctatgtcat caaactcaat aaacttaatc ggcgagtttg aactattata attaacattg
     8521 agtgctttta acttttggtt aaaatattta ctcaaactat ttaatttagt gcgtctatca
     8581 tcagcattta ttaacttttc aatgtttctt tttataaaat acaaattata aattggcaaa
     8641 ctacttgagt agatgagtgg tctaccgtga ttaattaaca tatccttcac atcatttgaa
     8701 cttaaaatca cacccccgta tgcaccacat gctttagata aactagaagt gagtatatct
     8761 acaccttgat aattcgagta attctctatt ccaaaactat gtgaaacatc gagtatcagt
     8821 gttgcgttat atttatgctt taatgagact aattgaccaa tatccacaac gtcgccattc
     8881 gttgaaaata cactatcaga tatgattatt tttggtatat tttgattagg gtatttctct
     8941 aacctttttt ctaaatcagc aatatctaaa tgcttatata tcactttttc taaaccactt
     9001 aacttaatac cgtcaataat actcgcatga ttttcttgat ctgaaaacac gacacaattt
     9061 gtatttttga aaatattaaa taacgccaaa ttagcatcat aaccactatt taagatagta
     9121 catgcactat atccgagcca acctgctaac attgtttcaa tttcttcata agctgtcgaa
     9181 cttccactaa ttaatcttga acttgataag tgataactat acttccgcat aaatctttcg
     9241 aaatcatcct tatcaaacgc tatttgacct aatcctaaat aatcattaga tgtatagttc
     9301 gtacatctct tattttctac ttcaatatac tgtctatcta tataccctac cgatttaagc
     9361 gaccgatata accctttctg ttgtaataaa tcaatttgct cttgaaactt cattcttgtt
     9421 ttccttattt tcacaagtgt cataatcaat ttcaaagcct aaatcattaa tcatatcgta
     9481 gtctaattgg ttcggttgcc caccagtaat tagataatca ccgacaaata ttgaattcgc
     9541 cgcttttaat gctaatggct gtaacgaacg taagttgacc tctcttcctc cagcaatacg
     9601 aatttctttc gtaggattga ttaatcggaa taatgctacg attcttaaac atttcattgg
     9661 tgttaaatca tccatgcttc caaactttgt gcctttgatt ggatgcaaaa aattaatcgg
     9721 aatactgtcg gcatccattt cttttaaagc aaatgccata tcaacaatat cttgattaga
     9781 ttctcccata ccacaaatca cgccagaaca tggtgatata ttattcgctt tcattagttc
     9841 tatcgtatct gttctatctt tataactatg cgttgtcacg acgttatcat ggtaattttc
     9901 acttgtatta atattgtggt tatatctgtc tacaccagct gacttaagtt tcttagcttg
     9961 ttcgtcattc gttaatccta aacatgcaca gatttttagt tgcgggtgtt gagatttaat
    10021 cgttcttaca gtattactaa tatgatcaac ttctttatcg ctcggtcctc taccactcat
    10081 aacaatacaa tatgttccaa tatgattatc atgtgccacc tttgctccat cgataatttg
    10141 ttcctctgga attaaagcat atcgctgttt ttgtttaata tctcgtgatt gtccacagta
    10201 cccacaattt tcaggacata taccactttt agcatttaaa atcatgttta attttacttt
    10261 tttaccaaaa taatgttttc ttaaaatgta cgcctcattt aataaatcta aggtatcaat
    10321 attagtatcc tcataaattt tcaatacagt ctcttttgtt aattgttccc cttgtaatat
    10381 gcgtttagcc aaattcatat taacacttcc tatctaaaat gttatgtact gcctttttaa
    10441 aaatacgtac catcgttttc acttctttat ttgacatact taacactggc acaaacgtaa
    10501 tgacattttc taaatttcta atcattagcc cgttttcttt acaattacgt acaatacgtt
    10561 caactttttc aatttctaac ggcgttttac tatctttatc tgtaactaat tcaactccaa
    10621 acattaagcc tcgtcctctt acatcaccaa catttcgatg acgcttcagc gcatgtaact
    10681 gtttctcaag tgttgatgat gtcgtttcaa tatgtgacaa taacttacgt ttttcataaa
    10741 gtcttatatt ttctaatgca accgtacaaa cgatttgatt tccggtgtat gtatgaccat
    10801 ggaaaaaggt attcacacca tgcgaatcac ttaaaaatgc attgtatatt tttttagatg
    10861 tcaatgtagc tgcaagtggt aagtagccac cagtaatcgc cttacctaaa cacataatat
    10921 ccggttgaac atcttcatga ttgcatgcaa acatctttcc agttctccca aaaccaactg
    10981 ctacctcatc acaaattaat aagacatcgt attttttgca caatttctcg acttctttca
    11041 aaaagccttt aggatgaaca aacaagcctg tcgcaccttg aatcaacggt tccaatataa
    11101 accctgcgat ttcatcattt ctttcaatta gaatttgctc tatattcgtt aaaatagctg
    11161 tcatcatttc attttcagta tcgtaattac tgcgatagaa agatgggctt tctacttgaa
    11221 tattctcaaa tattaagtct ttaaatattt tatgaaaggt cttgatacca ccaacactta
    11281 ccgcaccaat cgtatcccca tgataaccgt gatttagcgt tataaacttg tttttcttgg
    11341 catatttttc tctatcaata tttttccaat actgatatgc catctttatt gcgatttcaa
    11401 cagacgcact gcctgtatca gaataaaata cttttcttag attacttggc gtgatttcga
    11461 ttaatttttc cgcaagttct attgacggaa tatttgatga tcctagcagc gtagaatggg
    11521 caattttatt gagttgcttt ttaattacct tatttaagta tttgttatta tgaccatgca
    11581 cattgaccca caacgatgca taaccatcta aatatttatt gccattcgta tcgtaaaggt
    11641 aactaccctt tcctttttca atgatgattg cttcttcttt gctatataca cccatttgtg
    11701 taaatggatg ccaaacatat tctgagtctt tttgtttaag ttgttgtgtg taattcattt
    11761 ttaactccta ttaattgttt taaaaatgct tctgagaaat tttcatacgt ggcatgttct
    11821 tcaaatgtat agactgtttt atttgttaat tttccaatcg tcatttgatt gtctttttca
    11881 atatagctgt ctgtatagcg attcattatt aaaaaattac tcgccgatac attctgatta
    11941 acataatctt ggtgaacaat ggcatcgcta atagcaccta attttgatgg caacacactg
    12001 atgacacaat ctgcacaatc attgattaga tctttagtca tgtagaaatc atctgtacct
    12061 tcatatattg gtacggcaat tcccccagca ccctcaatta agataaaatc aaattcctta
    12121 tctaaaactt ttaccttatc taatacacgc tgcttattta gaaaaatttg atctgtcatt
    12181 ttaaatgcaa ggtgtggtga tacaggttgc ttaaaagtat aaagtgacgt tatgtcatag
    12241 cttaaatcac attcattttt aaatacttct aaatctggaa acgtcccgtc ttggcgttcc
    12301 tcagtttgaa atggtttaaa aatacaaact cgatgaccac gtgttttcaa agcatggtat
    12361 aaatgctttg taacataggt tttgcctaca tcagtattcg tacttgtaat aaaaatcctc
    12421 ataaaaataa ccacctcaat ccaaatgtaa acttttataa ttaataagtt tacatttaag
    12481 gcgcatgttt ttcaagaaaa catgaattta aacttctcag tataactata aattttggca
    12541 aaattttgac cttgctattg aaatgactaa gacttcttaa ggaattcggg cagatataaa
    12601 cagcgattcg ataaaatatg tcagttaaaa ttaataattg cattatttct taataaaaag
    12661 acatcctagc tttaccatct tgtgaaacaa tccattaatt gtaatattac gttgagcgcc
    12721 ttgttcatca atgctataat tagttacata ataaattgaa catctaaata caccaaatcc
    12781 cctcactatt gccctagtga ggggatttat tttgctgttg attatctatc acccttttta
    12841 tttccaaatt ctgattccat attaaactat tatttataat ttccagcctg ctgatttact
    12901 ttttattctt ataaagtctt gatacattcc tggttttcga attaattcat catgactacc
    12961 tttttgaatt atttcacctt cattgagcac tataatctga tctgcatttt taatagtttc
    13021 aagtttatgt gcaattgtaa ttactgtttt gcctttactt aattcattaa ttgccgtttg
    13081 aatcagctgt tcattttcag ggtcaatact tgcagttgct tcatctaaaa taattattgg
    13141 tgcatctttt aatatcgccc tagcaataga aatcctttgc ttttctccgc cagataaatt
    13201 actacctttt tcatttagca ttgtttgata tccctcaggt aatgacatga tgaagtcgtg
    13261 gcaacatgct tgcttcgctg cacgaataat ttcttctttc gttgcacctg gattgccaaa
    13321 caatatgtta ttttcaatcg tatcattaaa taaatacacc ttttgaaata ctgcactaat
    13381 tttcgacatt aacgtactta atgtcatatc tttaatatca acaccatcga tgcgaatatt
    13441 tccatcatcg atatcataaa agcgcaagag taagtgacat aatgtagatt tcccacttcc
    13501 tgaaggtcca attattgctg ttgatgtttg tgtaggtatc tcaaaattca cattctttat
    13561 cacttgttta tcatcatatg aaaagttcac gttttgaaaa gcgatatttt catttttaat
    13621 cgtcaaattt tttccattct catccagttc tggagcattt tttatctttt caatatcatc
    13681 tatcgtcata tctatcattt caagtacgtg tgcagcacta ttaatatttt caacactatc
    13741 aaatatcaca aatgaaaaaa ttgaaatcat aataagggta ggtaaatcaa tactatgatt
    13801 catatataat aagcatgcaa ctaatacaat cattattgaa acaactttta aactaagtaa
    13861 atgcaataaa ttgaatggga tatattgcat ttctattttt gtatttatac gtttactttc
    13921 attgactgct tggttaaaac ttttaagact cgtattttct ttcgcgaatg attttattac
    13981 ttgaatacca cgaataacct ccaatacctt ttccactaat tggttttgta cattatgata
    14041 cgctggcgca ttttgtcgac tctttctttc taataattga atcgcaaaaa atgatagtaa
    14101 tacgccaatg catgctaata atgatacttg ccacgaaact acaagtagag acaatatgag
    14161 tactgtaatt aatatgtatc cattaacaac aacgtccacc attttcatag caaagttttc
    14221 taaaaaggtt aaatctgttg ttactattgt tgttaactca tttgaatgat gcgaattaaa
    14281 ataacctaac cttacatttt tcaatttatc ccctatatct aaacgttctt tcgcactcat
    14341 ttcataagcg atgctctcat ggcttttgct tttgaaatat gctgtaataa atcgtccaat
    14401 cactaataaa accatgataa ttacaacatt caatatatct ttcatataaa taggtttatg
    14461 agatagcaca ttattaaata tttttgcagc taaaaagata ggtaacgcaa taaaaatagc
    14521 atttaaaaat gacatgctaa atcctaaaat cattcttgct ttatatggtc ttatccagtt
    14581 taaaatttta aatgtaattt gaaacatatt tgacctcctt ttctcattag tttaattatg
    14641 tccagtattg attccccaat cttttgtatg catgtgcgta tcccacattt tcttataatt
    14701 accgtttaat tttagcaata agtgatgtga ccctttttct aaaatttgtt gtttacctaa
    14761 aacaataatt tgatctgcat gttgaatcgt agataaccga tgtgcaataa caatcaatgt
    14821 tttatcctgc gtcaacacat ttaatgcttc ttgaattttt tgttcgttgt ctggatcaac
    14881 gtaaacagtc gcttcatcta atacaatgat aggcgcatct tttaatatca ttcttgcaat
    14941 agtgactcgt tgtttttcac caccagacaa tttatctccc actgtaccaa cattcgtatc
    15001 atatccatct ggcaactttt caataaattc atgacattgt gctaacttgg cagctttttc
    15061 aactgcctca tccgtagctt ctggattgcc aagtttaata ttttctttaa aagtaagatt
    15121 taataaaaag ttatcttgtc caacaaaacc aactaaatcg ttaagttgtt tcgattcaat
    15181 atcttttata tttataccgc caatcgtaat ttcacctgaa gtcacatccc agtatcgtga
    15241 tataagcttg gcaatggttg acttaccgct accagatgcc ccgacgatag ctgtgaaatt
    15301 attttctggt actgtaaatg ataaatgctt aaagaccaga tcgtccttat ctttgttata
    15361 tgaaaatcca acattattaa aagcaatttc ataatgttga ggcttcttaa attttgttga
    15421 caatactaac tcttctagac ttaatatttg attcacttca gttaatgcgt attgtataga
    15481 ctttaaatga tttacataat tagtaaaatt cttaatcggt gctactacac ctaaagataa
    15541 tacgatgcat aggaaaaatt ccgcatagtt taattggttg atagatatca aatacatgcc
    15601 gaccggtaaa atccctaaaa atgttgaagg taatacacta gctcctaaat tcatataccc
    15661 ccatgtattt ttaaaccaat tcaaagtgtg aatcttataa ttatctactg catctttata
    15721 ttttttatat gaactttgag attgattaaa tgttttaatc acttcaatgc cttcgataaa
    15781 ctctacaatc gcgctattca tataattatt cgatttcatt tgttcagcat atgtctcatt
    15841 aaatccagac ataacttttt taaaagcgaa aattgaaatt ggtatcgtta ctaataaggc
    15901 actagccata cgccaatcaa tgagcattat gtataaaaag atagcagctg acaaaagtaa
    15961 gtttcctata acttcaggaa tcatatgtgc taaaggtaat tctattgttt caaccttatc
    16021 gacaaatata ttttttaatt cacctatttt cttagattcc actacgccta aagggagacg
    16081 cattaatttt tgagctaatt ttttacgaat ttcagataaa atttcatatg ccgtaatatg
    16141 tgatagcatc gttgacgctc caaaacaaca cacttgtgaa atataagcga ttaaagcaat
    16201 aaagatataa accataatcg aattaatcgt atatgtattg ttaatcatca ttaaaataat
    16261 tttaaatact gcccaataag gaactaatcc agaaaagaca ctgatgatag acaacaaaat
    16321 tgataacata attttccatt tactatcaga agcaaatccg ctgagtatct ttatccaatt
    16381 tgtctgattg ttcatcgtat atgtaactcc tttcaattaa taatctaaat taagccgctt
    16441 atattattta tttcactg
//
LOCUS       DALAGF010000061        16375 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.061, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000061 DALAGF010000000
VERSION     DALAGF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16375
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.061"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(111..1079)
                     /locus_tag="O2692_001736"
     CDS             complement(111..1079)
                     /locus_tag="O2692_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCZ4299365.1"
                     /translation="MKPFESHNKQLKILRRRGMEVPSSAKRDLENENYYNIINGYKDL
                     FLELDVNGNFLVPDKYKQGTHFKEVFSLYKLDRKFRNVLLEYLLVFETHIKSRISYYF
                     SEKYREPHSYLYFKNYSSDTSKTDSIVKMVATFSSVMSNRKNKPLKHYINTHNGVPLW
                     ILVNYLTLGNVSKTYSNLDDDLRLEVAKDYKRKLERDYKTRVQITPSDVDSILQQAHM
                     FRNVCAHEERLYDYKIDRAKSRANIFANYNKIYDKEYVPTMNGSYVFDLLISLCLFLN
                     KHDYIKLMKNMDKLISNYSHSFYTITIDDLYTKMNFPDQTKILDML"
     gene            complement(1358..1510)
                     /locus_tag="O2692_001737"
                     /pseudo
     CDS             complement(1358..1510)
                     /locus_tag="O2692_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            complement(1510..2001)
                     /locus_tag="O2692_001738"
     CDS             complement(1510..2001)
                     /locus_tag="O2692_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCZ4299366.1"
                     /translation="MVSDFESNDKITEVELLMHYNPKVINSKIKAMRSQIESLYHLNM
                     NHVITNDNDMLVSVSYPLDKLVLYIIEEKDKLEYYMKTAQDRLNLFKNIIKNYSENEQ
                     QDVMRYMLSSGKVKNEGVIERLKVDIYKVESRKRQERQNQREELHRIEFNKHLEQVKK
                     ELS"
     gene            complement(2185..3243)
                     /locus_tag="O2692_001739"
     CDS             complement(2185..3243)
                     /locus_tag="O2692_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HCZ4299367.1"
                     /translation="MKEFKEQFGYQLSNFDDMDIKGYANLYQKDIGKDVSMIEQGLKQ
                     LSITETEVLLPEQINKKLLGVLNMNEVNQSSNTWVGTLTKQLVSSENNFNIQELPTAR
                     KEVFKELLVNCELPQSLREVITITDDEHVESIPSLTYIKDKLATNGIELSLNGSSKYF
                     DRREGHIYTEVSDSVVHGSDRTLDDLLKEIFINECVAYETTLLLDKNNSSGLIDKGNQ
                     DLSLYNQGIKEVSNTSIYDGIKQAMKDIPQTFRRNVSIVMNTEHHDKLIKELAQMGLG
                     GLAGDLSNLFNVTNVVVTDDAHDVFVGDFGHAIYAKYEPIMYNKKKQALKGIYQFALN
                     YVFDIKIIPELLRIVNIK"
     gene            complement(3230..3436)
                     /locus_tag="O2692_001740"
     CDS             complement(3230..3436)
                     /locus_tag="O2692_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299368.1"
                     /translation="MLDHTNESVKRVFKYRDKQELLNKYHELLDKYFLHNISFYEEDK
                     NNERIYGLVVHTKNKLGDEDYERV"
     gene            complement(3661..4005)
                     /locus_tag="O2692_001741"
     CDS             complement(3661..4005)
                     /locus_tag="O2692_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCZ4299369.1"
                     /translation="MNRNRMKQIILEYIKNNDSTSFVEIENVFEEQGFKYKGNGAYTS
                     GNHKNIIFWMGWNEEAFNIVADLKRDGLIEMQICPPMYYLIDGKGLRLPIVKNKNIKI
                     DHWLPVTFSLVN"
     gene            complement(4676..7048)
                     /locus_tag="O2692_001742"
     CDS             complement(4676..7048)
                     /locus_tag="O2692_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCZ4299370.1"
                     /translation="MAIKKKDRIIGVKELEIPQELKLVPNWVLWRAEWNEKQQNFGKV
                     PYSINGYRASTTNKKTWCDFESVSIEYEVDEQYSGIGFVLSDGNNFVCLDIDNAIDKK
                     GQINSELALKMMQLTYCEKSPSGTGLHCFFKGKLPDNRKKKRTDLDIELYDSARFMTV
                     TGCTIGQSDICDNQEVLNTLVDEYFKENLPANEVVREESNTNIQLSDEDIINIMMKSK
                     QKDKIKDLLQGTYESYFESSSEAVQSLLHYLAFYTGKNKQQMERIFLNYNNLTDKWES
                     KRGNTTWGQLELDKAIKNQKTIYTKSIDEFNVIPQGSKDVKQLLNQLGHEERTKMEEN
                     WIEEGKRGRKPTTISPIKCAYILNEHLTFILFDDEENTKLAMYQFDEGIYTQNTTIIK
                     RVISYLEPKHNSNKADEVIYHLTNMVDIKEKTNSPYLIPVKNGVFNRKTKQLESFTPD
                     YIFTSKIDTSYVRQDIVPEINGWNIDRWIEEIACNDNQVVKLLWQVINDSMNGNYTRK
                     KAIFFVGDGNNGKGTFQELLSNVIGYSNIASLKVNEFDERFKLSVLEGKTAVIGDDVP
                     VGVYVDDSSNFKSVVTGDPVLVEFKNKPLYRATFKCTVIQSTNGMPKFKDKTGGTLRR
                     LLIVPFNANFNGIKENFKIKEDYIKNQQVLEYVLYKAINLDFETFDIPDASKKMLEVF
                     KEDNDPVYGFKVNMFDQWTIRKVPKYIVYAFYKEYCDENGYNALSSNKFYKQFEHYLE
                     NYWKTDAQRRYDNEELAKRIYNFNDNRNYIEPIESGKNYKSYEKVKLKAI"
     gene            complement(7140..7388)
                     /locus_tag="O2692_001743"
     CDS             complement(7140..7388)
                     /locus_tag="O2692_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HCZ4299371.1"
                     /translation="MNWEIKNLMCDLKILKEKFEDLKDNHGSHFEDLYLHEPNHTLNK
                     DDAIKEGFSYHERRIHNDQMFDALEKASSFADQSQDNA"
     gene            complement(7389..7772)
                     /locus_tag="O2692_001744"
     CDS             complement(7389..7772)
                     /locus_tag="O2692_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCZ4299372.1"
                     /translation="MEIKQKYQLSKVVKILEVVLYEEDKFQSDKDYHYQDKALYEYAL
                     KLVHNGLFNILAELDFEDEAFLILDEVTMTLSDVMKETQHVYRYSVIDEKGEHKHTTD
                     RKGHVIGMLEWALDYIVGNIEVEEL"
     gene            complement(7774..7977)
                     /locus_tag="O2692_001745"
     CDS             complement(7774..7977)
                     /locus_tag="O2692_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCZ4299373.1"
                     /translation="MRKQVIITKTVVGWYNIKDTQHNLMLNIPPKVFEQYFPDVSKDV
                     QVACLEMDLSKITEIKNKKKVGS"
     gene            complement(7944..8117)
                     /locus_tag="O2692_001746"
     CDS             complement(7944..8117)
                     /locus_tag="O2692_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCZ4299374.1"
                     /translation="MKMYLTYICLVSLLTILLLAISNMYVAFSVYGMMVTYGFNLTGE
                     ITTCENKLLLQKQ"
     gene            complement(8129..8401)
                     /locus_tag="O2692_001747"
     CDS             complement(8129..8401)
                     /locus_tag="O2692_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCZ4299375.1"
                     /translation="MSRTKLHDVPAKENTITEPKQVVVNPLFAKPNALASILGISYSS
                     VNRILKEWEKDSKGVDDLYYSLSSTMIVISIPRFEEYMKARHKKWM"
     gene            complement(8394..8657)
                     /locus_tag="O2692_001748"
     CDS             complement(8394..8657)
                     /locus_tag="O2692_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4299376.1"
                     /translation="MVLNLKRLRAERIACGITQDEMAHKMGWKTRTPYAKRENGIVDI
                     GANEFIKMAKILGYETNNLDIFFTNNVPGKERKNILKGGELNV"
     gene            8850..9182
                     /locus_tag="O2692_001749"
     CDS             8850..9182
                     /locus_tag="O2692_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4299377.1"
                     /translation="MRTNDEIITIIKTSMKEQNMSLSELARRVGVAKSAVSRYLNLTR
                     EFPLNRAEDFAKVLGIKTEYLLGFAEREESTKQDTIAAHLDGDFTEEELIEIRKYAEL
                     VRKAHRNQ"
     gene            9194..9652
                     /locus_tag="O2692_001750"
     CDS             9194..9652
                     /locus_tag="O2692_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCZ4299378.1"
                     /translation="MYLYEKMVIENKEIPIDDGKSLGNFEGLYDNGVILINKNLSETR
                     KAEVLYEELAHHKLTYGNILDQSKWINRKFENYARRHGFISAVPLREIVEAYNYGVRN
                     LYELSEYLQLSEKYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            9669..10397
                     /gene="seq"
                     /locus_tag="O2692_001751"
     CDS             9669..10397
                     /gene="seq"
                     /locus_tag="O2692_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type Q"
                     /protein_id="HCZ4299379.1"
                     /translation="MNKIFRVLTVSLFFFTFLIKNNLAYADVGVINLRNFYTNYQPET
                     LQGVSSGNFSTSHQLEYIDGKYTLYSQFHNEYEAKRLKDHKVDIFGISYSGLCNTKYM
                     YGGITLANQNLDKPRNIPINLWVNGKQNTISTDEVSTQKKEVTAQEIDIKLRKYLQNE
                     YNIYGFNKTKKGQEYGYQSKFNSGFNKGKITFHLNNETSFTYDLFYTGTGQAESFLKI
                     YNDNKTIDAENFHLDVEISYEKTE"
     gene            10421..11149
                     /gene="sek"
                     /locus_tag="O2692_001752"
     CDS             10421..11149
                     /gene="sek"
                     /locus_tag="O2692_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type K"
                     /protein_id="HCZ4299380.1"
                     /translation="MKKLIVIILINIITLSVSNSASAQGDIGIDNLRNFYTKKDFVDL
                     KDVKDNDTPIANQLQFSNVSYDLISESKDFNKFSNFKGKKLDVFGISYNGQCNNKYIY
                     GGVTATNEYLDKPRNIPINIWINGNHKTISTNKVSTNKKFVTAQEIDVKLRKYLQEEY
                     NIYGHNGTKKGEEYGHKSKFYSGFNIGKVTFHLNNNDTFSYDLFYTGDDGLPKSFLKI
                     YEDNKTVESEKFHLDVDISYKEMI"
     gene            11237..12457
                     /locus_tag="O2692_001753"
     CDS             11237..12457
                     /locus_tag="O2692_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCZ4299381.1"
                     /translation="MWLEKFKNKNKETKYRYYEKYKDPLTAKWRRVSVVLNKNGKQSQ
                     KEAQRLLNERIEEKLNDKKPTTLKSLTFHVACDEWFQNYIKTSGSKRTTIKTKLSKLN
                     TLKKFVDEDILINKITLSYAQQVFDEMDSKGYVYQVNKDALSIFKNVFEYTRRIYKLQ
                     DLEFLKDITLNKRIKSYDEVKAKRNKYLELNEIESIIKDINTKAQKMHSGIHKRFYLF
                     VALMTEFQALNGMRIGEMLAIQNEDIDFDNKSLNINGTIHWFHDESGGFGVKDTTKTE
                     SSYRTIGLSSRSCEILKKAILENKKDSKWNDGYLNRNFVFTNHKGNPMQTERFNKILR
                     EAAKDVGIDKEVSSHILRHSHISLLSQQGVSLKAIMDRVGHSDHRTTLSIYSHVTEQM
                     DKDMMNKLEQVKLG"
     gene            complement(12600..13421)
                     /locus_tag="O2692_001754"
     CDS             complement(12600..13421)
                     /locus_tag="O2692_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCZ4299382.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            complement(13439..14134)
                     /locus_tag="O2692_001755"
     CDS             complement(13439..14134)
                     /locus_tag="O2692_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4299383.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            complement(14127..15152)
                     /locus_tag="O2692_001756"
     CDS             complement(14127..15152)
                     /locus_tag="O2692_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCZ4299384.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     regulatory      complement(15241..15344)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(15402..15698)
                     /locus_tag="O2692_001757"
     CDS             complement(15402..15698)
                     /locus_tag="O2692_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCZ4299385.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     gene            complement(15691..16077)
                     /locus_tag="O2692_001758"
     CDS             complement(15691..16077)
                     /locus_tag="O2692_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCZ4299386.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
BASE COUNT         5381 a         2835 c         2196 g         5963 t
ORIGIN      
        1 atactttatc tatttctata tacacactca tccctcactg caacacaggg cgtttctcag
       61 cgtcttaaaa taaaaaaacg ccactcgtaa gtgacattaa aaaatatctt ttataacata
      121 tccagtattt ttgtttgatc tggaaaattc atttttgtat ataggtcatc tatagtaatt
      181 gtatagaaag aatgtgaata attacttatt agtttatcca tatttttcat caattttatg
      241 taatcatgtt tattcaaaaa tagacatagt gaaatcaaca aatcgaatac ataactacca
      301 ttcattgtag gaacgtattc tttatcgtat attttgttat aattggcgaa tatattagct
      361 ctacttttag ccctgtctat tttataatca tacaatcttt cttcatgcgc acacacgtta
      421 cgaaacatat gtgcttgttg tagtatactg tctacatctg atggagttat ttgaacacgt
      481 gttttataat ctctttccaa tttcctttta tagtctttag caacttccaa tcgaagatca
      541 tcatccaaat tggaatacgt ttttgaaaca ttacctaaag ttaaataatt caccaatatc
      601 catagtggca ctccattatg agtattaata taatgtttta atggtttatt ttttctatta
      661 ctcataaccg agctaaatgt agcaaccatt ttcacgatgc tatctgtctt acttgtgtca
      721 gatgaataat ttttaaagta taaatatgaa tgtggttctc tatatttttc gctaaaataa
      781 tatgaaattc ttgatttaat atgagtttca aatactaaca aatactctaa taaaacattc
      841 ctaaattttc tatctagttt gtataaagaa aagacttctt taaaatgagt accttgctta
      901 tatttatcag gaaccaaaaa attaccatta acatctagtt ctaaaaataa atctttataa
      961 ccatttatga tattataata attttcattt tctaaatctc ttttagcact actcggtact
     1021 tccattcctc ttcttcttag aattttcaat tgtttattat gactttcaaa tggcttcata
     1081 aatttacccc tataaaagta gccataaccc gaatagagtt atggctagat cgttatatat
     1141 atataataca tttaatttta tcggttgtaa ataaataaga aataacaaaa agagatattt
     1201 tacacaatta cctcctttat tttattgcta ctcctcaaac ccaccaatat tatcaataaa
     1261 cactggtgtt gttacattta agtctacttt ctcagtaaat aagctatggt atcaaactaa
     1321 ttagtattaa tttataaata gcatagcttc attttcttca atcctctaac ggtatatcat
     1381 ccacaatcac agtatgatta tggttagcgt tagatacatc ttgcactgcc ttatctaatt
     1441 cctcatcatc tccgtcccat tcaccaatat taatgaatat aggaatattc ccgttaatat
     1501 catgcttatc tatgaaagtt cttttttaac ttgttctaaa tgcttattaa actctattcg
     1561 atgtaattct tctcgctggt tttgtcgttc ttgacgcttt ctactttcaa ctttataaat
     1621 atctactttt aaacgttcaa tgactccttc atttttaact ttcccactac ttaacatgta
     1681 cctcataaca tcctgttgtt cattttcaga atagtttttg atgatatttt taaacaaatt
     1741 tagcctatcc tgagctgttt tcatgtaata ttcaagttta tctttctctt ctataatata
     1801 aagtactaat ttatctaatg gatatgatac agataccagc atatcgttat cattcgtgat
     1861 cacatggttc atgttcaagt gatataagct ttctatttgt gatctcatcg ctttaatttt
     1921 gctattaatg actttcggat tgtaatgcat taaaagttct acttcagtga ttttatcatt
     1981 gctttcaaag tcagatacca aataaccaca cctttacata tcgaatttaa tatttataag
     2041 tatctgaaaa agggataaat caagttatcc ccctttcata aaagtttatc cgcgttgcaa
     2101 gcgaagggcc cccgccggta cccggcgaaa aaacatttta agccgatggg cagggggcta
     2161 taaaatttta tttaaataat ttttttattt aatattaact atgcgcaata actctggaat
     2221 tatttttata tcaaacacat agttcaatgc aaattgataa atacctttta atgcttgttt
     2281 ctttttgtta tacataatag gttcgtattt agcatatatg gcatgtccaa agtctccaac
     2341 aaagacatca tgtgcatcat cagtaaccac tacattagtc acattaaata ggttacttaa
     2401 atcacctgca agtccaccta atcccatctg tgccaactct ttgatgagct tatcatgatg
     2461 ttctgtgttc atcacaatag atacattacg cctaaacgtt tgtggtatat ctttcatcgc
     2521 ttgcttgata ccatcgtata ttgatgtgtt acttacttct ttgatacctt gattataaag
     2581 tgataagtct tggttacctt tatcaattaa accacttgaa ttattcttat ctaataacaa
     2641 tgttgtttca tatgcgacac actcattaat aaatatctct ttaagtagat catctaatgt
     2701 tctatcagag ccatgaacta cactgtctga tacttctgtg tagatatgtc cctcacgtct
     2761 atcaaagtat ttactactgc cattaagtga tagctcaata ccgttagtag ctaacttatc
     2821 tttaatgtat gttagtgatg gtatagattc aacatgttca tcatcagtaa tggtaatcac
     2881 ctcacgtagt gattgaggta gctcacaatt caccaataac tctttaaata cttctttacg
     2941 tgcagttggt aactcttgaa tattaaagtt attctcacta ctaactaatt gttttgttaa
     3001 ggtacctacc catgtattac ttgattgatt tacttcattc atatttaaca ctcctaataa
     3061 ttttttgtta atttgctctg gtaataaaac ttctgtttca gtaatactta attgctttaa
     3121 cccctgttca atcatactta catctttacc tatatccttt tgatatagat tggcatatcc
     3181 tttaatatcc atatcatcaa agttagatag ttgataccca aattgttctt taaactcttt
     3241 cataatcttc atctcctaat ttgtttttag tatgaacaac taaaccatat atacgttcgt
     3301 tgtttttatc ttcttcatag aacgaaatgt tatgaagaaa gtatttgtct aataattcgt
     3361 gatacttatt taatagttct tgcttatctc tatacttaaa cacacgtttt acagattcat
     3421 ttgtatgatc tagcacgtct tgcctctctc cttttaatct gatagatata cattttaata
     3481 agttcaaaat cagcacgctt ttttgtatag cgccttacaa cgcgatttat atatttcgta
     3541 ttcattaatt gaacgttcac aggtgtatgt ttgattattt gatatgcgat tcttttatga
     3601 ttaataaata tacacactcc catatctata cttgatattt taaatgctta tctgcatgtt
     3661 ttaatttact aaactaaatg ttactggtag ccaatgatct atttttatat ttttattctt
     3721 tacaattggt agccttagac ctttaccatc aataagatag tacattggcg gacaaatttg
     3781 catttcaatt agcccgtcgc gttttaaatc tgccacaatg ttaaacgctt cttcattcca
     3841 acccatccaa aatataatat tcttatggtt gccactggta tatgcgccgt tacctttgta
     3901 tttaaaacct tgttcttcaa atacattctc aatctctaca aatgaagtgc tgtcattgtt
     3961 ctttatatat tctaagatga tttgtttcat tctatttcta ttcatgttac tctagtcctc
     4021 caatctatcc agttactaaa gcaaccacgt tagatactaa aaaccactga atttatgttt
     4081 ttcaataacc actgtaaaca cagtgatgtc aatacataca ggctgttggt tactgagtta
     4141 ctgaaaattc acggtatttt atataatctt ttattacctt aattctctat tatacatttc
     4201 tactaaaaag aaataattta tagtaactca gtaacttttt gtcaaaaaga ctaatatgaa
     4261 agtaaaaact gtggttactg aattctagaa ttttagtatc ttttagtgac ttaaacgttt
     4321 tttagtaact ttagtatctt aggcaatttt gtataactgc aagtctcttc cgattatgtg
     4381 caactgaaat ttacagctat gcaactcgtt ttaaattttc cagttgcaat ttttttctta
     4441 ctgtcacaag gggttaatcc acttttgcaa ctatgcaacc gaaatcacac aaattagttt
     4501 atataatttt tgatactatt tcttatttat attttggtta tttacttttc tactaataag
     4561 ttatagattt tgagttgctc agttgcataa gtgccctaaa cccttgatat aactgtgatt
     4621 ataggtgcaa cccgttttaa aattttcggt tgcattcggt tgcattcggt tgcaactata
     4681 ttgcttttag cttcaccttt tcatacgatt tatagttttt tccactttca ataggttcaa
     4741 tgtaatttct attgtcatta aagttgtata tcctcttagc aagttcttca ttgtcatatc
     4801 ttcgctgtgc atcagttttc caataattct ctaaataatg ttcaaattgt ttataaaact
     4861 tgtttgaact caatgcatta tagccatttt catcacaata ttctttataa aatgcgtata
     4921 caatatattt cggcactttt ctaatagtcc attgatcaaa catatttact ttaaacccat
     4981 aaactggatc gttatcttct ttaaatactt caagcatttt tttagatgca tcaggaatgt
     5041 caaaggtttc aaaatctaaa ttaattgctt tataaagcac atactctagc acttgctgat
     5101 tttttatata gtcttctttg attttaaaat tctctttaat gccattaaaa ttggcattaa
     5161 acggtactat caataacctt cttaaagtcc cgcctgtttt gtctttaaat ttaggcattc
     5221 cattcgttga ttgaataact gtacacttaa aagtcgctct atataagggt ttatttttaa
     5281 actcaaccaa cactggatcg ccagtaacta cacttttaaa gtttgaagaa tcatcgacat
     5341 acacaccaac tggtacatcg tctccaatta ctgctgtctt gccctctaac acactcaatt
     5401 taaaacgttc atcaaactca ttcactttta agctagcaat attgctataa cctattacat
     5461 tagacaataa ttcttgaaac gtccctttac cattatttcc atcaccaaca aagaaaatag
     5521 cctttttacg agtgtagtta ccattcattg agtcattaat tacttgccac aataatttaa
     5581 caacctgatt atcattacaa gcaatctctt ctatccaccg atctatattc cagccattta
     5641 tttcaggtac tatatcttgc cttacatacg atgtatctat tttactggta aatatataat
     5701 caggtgtaaa tgattctagt tgcttcgttt tacggttaaa tacaccattt ttaactggta
     5761 ttaagtatgg tgagttagtt ttctctttta tatctaccat attggttaaa tgataaataa
     5821 cttcatcagc tttgttgcta ttatgtttgg gctctaaata ggaaatcact cgttttataa
     5881 ttgtagtgtt ctgtgtatat atcccttcat caaattgata catagctaac ttagtatttt
     5941 cttcatcatc aaaaagtata aatgttaaat gctcattcaa aatatatgca cattttatag
     6001 ggctaattgt tgtaggcttt cgccctcgtt ttccttcttc aatccaattt tcttccattt
     6061 ttgttctttc ttcatgcccc aattgattca acaactgttt aacatcttta ctcccctgtg
     6121 gtataacatt aaattcatct atcgatttag tataaattgt cttttgattc tttatagctt
     6181 tatctaactc aagctgtccc caagtcgtat tacctcgttt actttcccat ttatccgtaa
     6241 gattattgta gtttaaaaat atacgctcca tttgctgttt gtttttacct gtgtaaaacg
     6301 ctaagtagtg taataagctt tgtactgctt cgcttgaact ctcaaaatat gattcatatg
     6361 tgccttgtaa tagatcttta attttatctt tttgtttaga tttcatcata atgtttataa
     6421 tatcttcatc agataattgt atattggtat tagattcctc tctcacaacc tcatttgctg
     6481 gcaaattctc tttaaaatat tcatcaacta gagtatttaa tacttcttga ttatcacaaa
     6541 tatcactttg accaattgtg catcctgtaa cagtcataaa ccttgctgaa tcatataact
     6601 ctatgtctaa atctgttctt ttctttttac ggttatctgg tagtttacct ttaaagaaac
     6661 aatgtaatcc tgtaccactt ggagactttt cacaatatgt gagttgcatc atttttagtg
     6721 ctaattcaga attgatttgt ccttttttat caattgcatt atcaatatct aggcaaacaa
     6781 aattattacc atcacttaat acaaaaccta taccgctata ttgctcatca acttcatatt
     6841 caatacttac actttcaaag tcacaccatg tttttttatt ggttgtacta gctctgtaac
     6901 cattaatact atatggtact tttccaaaat tttgttgctt ctcgttccat tcagctcgcc
     6961 ataataccca attgggcact aattttaatt cttgcggtat ttctaattct ttaactccaa
     7021 taattctatc ttttttctta attgccacag aatgcctcct ttctaatctg atttctctgt
     7081 attttcaatg caactaatgt taaaatatta atggtatgat tttataaatt tttatttttt
     7141 tatgcgttat cttggctttg gtcggcgaaa gatgacgctt tttctaatgc atcaaacatt
     7201 tgatcattgt gaatacgtct ctcatgatat gaaaatcctt ctttaatagc atcgtcttta
     7261 tttaaggtat gatttggctc atgtagatat aaatcttcaa aatgcgagcc atgattatcc
     7321 tttaaatcct caaacttttc tttcaatatc tttaaatcac acattaaatt tttaatttcc
     7381 caattcattt ataattcctc cacttcaata tttcccacaa tgtaatctaa tgcccactct
     7441 aacattccaa tcacgtgtcc tttgcgatct gttgtatgtt tatgttcacc tttttcgtct
     7501 atgacactat aacggtaaac gtgttgtgtt tctttcatga catcacttag cgtcattgtt
     7561 acttcatcaa gaattaaaaa tgcttcatct tcaaaatcta attcagcaag aatattgaac
     7621 aatccattat gaactaactt taaagcatat tcatataatg ctttatcctg ataatgatag
     7681 tccttatcgg attgaaactt atcttcctcg tataatacta cttctaatat tttaaccact
     7741 tttgataatt gatatttttg tttgatttcc atcttaacta cctacttttt tcttattttt
     7801 aatttctgta atttttgata aatccatttc taaacacgca acttgaacat ctttactaac
     7861 atcaggaaag tactgttcaa atacttttgg cggtatattt aacattaaat tatgttgagt
     7921 atctttaatg ttgtaccagc caactactgt ttttgtaata ataacttgtt ttcgcacgtt
     7981 gtaatctctc ctgttaaatt aaatccataa gttaccatca tgccgtacac actaaaagcg
     8041 acatacatgt tagatattgc tagtaataat attgttaaca atgaaactaa gcagatataa
     8101 gttaagtaca ttttcattgc cttgcctcct acatccattt tttatgacgc gccttcatgt
     8161 actcctcgaa tcgcggaata ctgataacaa tcattgttga tgataacgag taatataaat
     8221 catcaacacc tttagaatct ttttcccact cttttaaaat gcgattcacc gaactatatg
     8281 aaattcccaa aatactagct agtgcattag gtttcgcaaa caaaggattc actacaactt
     8341 gctttggttc tgtaattgta ttttctttag ctggtacatc atgcaatttt gttctagaca
     8401 tttaattcac ctccttttaa gatgtttttt cgttcttttc cgggaacgtt attggtaaaa
     8461 aaaatgtcta ggttatttgt ttcataacct aatatttttg ccattttaat aaattcattc
     8521 gctccaatat ctactattcc attttctctc tttgcataag gcgttcttgt tttccacccc
     8581 attttgtgag ccatttcatc ttgcgtaata ccacaagcta ttctttccgc tctcaatctt
     8641 tttaaattta gtaccacgtt tacacctcct atcgttctcg ttcgagaact aattacaatt
     8701 taccatttac gttctcgttc gtcaacaatt tttacttaaa aaaattcaaa aagttttttc
     8761 ttccttatat attgtattct tttgggaacg gtgttataat caaatcgttc acaaataaga
     8821 acaaatattc aattcaggag atacaagaaa tgagaactaa tgatgaaata atcacaataa
     8881 ttaaaacatc aatgaaagaa caaaatatgt cactaagtga attagctcgt cgtgtaggtg
     8941 tagcaaaatc agcagtatca agatatttaa atttaactag agagttccca ttgaatcgtg
     9001 ctgaagattt tgcgaaagta cttggaataa aaacagaata tttattagga tttgctgaac
     9061 gcgaagaatc tacaaaacaa gatactatcg ccgcgcactt agatggagat tttacagagg
     9121 aagaattaat tgaaattaga aagtatgcgg agttagttag aaaagcacat cgaaatcagt
     9181 aaggggttat tgcatgtatt tatacgaaaa gatggttatt gaaaacaaag agataccaat
     9241 tgatgatggg aaatctttag gtaattttga gggtctctat gacaatgggg ttattttaat
     9301 taataaaaat ttgtcagaaa cacgtaaagc agaagtgtta tatgaggaac ttgcccacca
     9361 caagttgacg tatggcaaca ttttagacca atcaaaatgg attaatagaa aatttgaaaa
     9421 ttatgcacgt agacacggct ttatctcagc tgttccatta cgtgaaattg tagaagctta
     9481 taattatggc gtacgtaact tgtatgagtt gtccgagtac ctacaactga gtgaaaaata
     9541 catattagaa gcgatagaac aatataaaaa gatatacggt attggtaccc actacggcga
     9601 gtattcaatt acatttgagc cgttgagagt ttttaaatat aaagaaatat aacaaaggag
     9661 aaatgaaaat gaataaaata tttagagtac tcactgttag cttgtttttc ttcacatttt
     9721 taataaaaaa caatctagca tatgctgatg taggggtaat caaccttaga aacttttata
     9781 ctaattatca accagaaacg cttcaaggag ttagttctgg aaatttttct acttctcatc
     9841 aattagagta tattgatgga aaatacactt tatattcaca gtttcataat gaatatgaag
     9901 cgaagagatt aaaagatcat aaagtagata tctttggaat aagttactca ggtctttgta
     9961 atacaaaata tatgtatggc ggaattacgt tggcgaatca aaatttagat aaacctagaa
    10021 atatacctat taatctctgg gtcaatggta agcaaaacac tatatctaca gacgaggttt
    10081 ctactcaaaa aaaagaggta actgctcaag agattgatat taagttacga aaatacctac
    10141 aaaacgaata caatatatat ggttttaata aaacaaaaaa aggtcaggaa tatggatatc
    10201 agtcaaaatt taattctgga tttaacaaag gaaaaattac tttccattta aataatgaaa
    10261 cttcttttac atacgatttg ttttacaccg gaactggtca agcagagagt tttttaaaaa
    10321 tttacaatga taataaaact atagatgcag agaattttca tttggatgta gagatttcat
    10381 atgagaagac tgaataaata gaatttaagg agaaaatgca atgaaaaaat taatagttat
    10441 catattaata aatataataa ctttaagtgt ctctaatagt gccagcgctc aaggtgatat
    10501 aggaattgat aatctcagga atttttatac aaaaaaagac tttgtagatt taaaagatgt
    10561 aaaagacaat gatactccta tagctaatca actacaattt tcaaatgtat cttatgattt
    10621 aatttcagaa tcaaaagatt ttaataaatt tagtaatttc aagggaaaaa aacttgatgt
    10681 ttttggtatt agttataatg gccagtgtaa caataaatac atatatggtg gagtcacagc
    10741 tactaacgaa tatctagata aacctagaaa tatccctata aatatatgga tcaatggaaa
    10801 tcacaaaact atttctacca ataaagtttc gacaaataaa aaatttgtta ccgctcaaga
    10861 gattgatgtc aaattaagaa agtaccttca agaagaatac aacatttatg gacataacgg
    10921 cactaaaaaa ggagaagaat atggtcataa atcaaaattt tattctgggt ttaatattgg
    10981 taaagtaacg ttccatttaa ataataatga cactttttca tatgatttat tctacacagg
    11041 agatgatggg ttaccaaaaa gttttttaaa aatttacgaa gacaataaaa ctgtagagtc
    11101 tgagaaattc catttggatg tcgatatttc ttataaagaa atgatataaa tctattaatt
    11161 atgtcataat cattattttc cgggtagcat gcctaccctt attatttttt gccaattttg
    11221 aggagggaga agcaaaatgt ggttagaaaa atttaaaaat aagaataaag aaactaaata
    11281 tagatattac gagaagtaca aagaccctct cactgccaaa tggagacgtg ttagcgtggt
    11341 acttaataag aatggtaagc aatcacaaaa agaagctcaa agactcttaa atgagcgtat
    11401 agaagaaaaa ctgaatgata agaaacctac tacacttaag tcactaactt tccatgtcgc
    11461 atgtgatgag tggtttcaga attatatcaa aacgtctggg tcaaaaagaa caactattaa
    11521 aactaaattg agtaagctaa acactttgaa gaaatttgta gatgaagata ttttaatcaa
    11581 taaaataaca ctttcatatg cgcaacaagt tttcgatgaa atggatagta aaggttatgt
    11641 atatcaagta aacaaagatg cgttaagcat attcaaaaat gtatttgaat acactagacg
    11701 tatctataaa ctgcaagatt tagaattttt aaaagatata acattaaata aaagaattaa
    11761 gtcttatgat gaagtgaaag ctaaacgtaa taagtacctc gaattaaatg aaatagaatc
    11821 tatcatcaaa gatattaata cgaaggctca gaagatgcac tcaggtatcc ataagcggtt
    11881 ttatttattc gttgcactca tgacagaatt ccaagcctta aatggtatgc gtattggtga
    11941 aatgttagcc attcaaaatg aagatataga ctttgataat aagagtttaa atattaacgg
    12001 aacaatccat tggtttcacg atgaatctgg tggattcggt gtaaaagata ccactaaaac
    12061 agagtctagt tatagaacaa ttggattgag tagcagaagt tgcgagatat taaagaaagc
    12121 aatactggag aacaaaaagg attcaaaatg gaatgatgga tatctaaata gaaattttgt
    12181 atttactaat cataaaggca acccaatgca gactgaaaga ttcaataaaa tccttaggga
    12241 agcagctaaa gatgttggta ttgataaaga agtttcttca catatattaa gacatagcca
    12301 catatcatta ctctctcaac aaggcgtgtc acttaaagct attatggatc gtgtaggcca
    12361 ttccgaccac cgtacaacac tttccattta ttctcacgtt actgaacaga tggataagga
    12421 tatgatgaac aaacttgaac aggtaaaact tggataatgc gctcaaatct gagcgtattt
    12481 ttttgtttaa aattcgataa tttgcatata ctgccccttt tgtgcccttt tttattttca
    12541 ataccacaaa aataacccct taatcgttgt tattaaaggg tatttttatt taattggtat
    12601 tatttagcag gaataactgc accattgtat ttttcattaa tgaagtcttg aatatcttta
    12661 gattgtaata cttcaattaa tgctttgatt ttcttatcat cttgatgtcc ttctttaaca
    12721 gcaattaagt ttgcataagg attatctttc gcactttcta cagcaataga atctttttta
    12781 ggatttagtt tttgttcgat tgcaaagttc gaattaatga taacagcatc agcgtcttca
    12841 ttttgataaa ttttaggtaa gaattctgct gattgtttat tattaaactt aatatctttt
    12901 ttattctctg taatatcact aaacttagca tcttcaattt ttacgccttt tttgatttta
    12961 attaaacctg catcaacgaa gaattttaag aaacgtcctt gttcagctgg attattagac
    13021 acatagactg ttgcaccttt tggtaattct tttaaacttt tatacttttt agagtataca
    13081 gccataggtt ctaagtgaac atcaccggca cttacgattt tgtaaccttt atcctttttc
    13141 tctgtgttta aatatggtgt atgttggaaa tagtttgcgt caatttcacc tttgtctagt
    13201 aatttattag gtgtagtgta atcgttaatt gttttaatat ctagttcata accttttttc
    13261 tctaataatg gttttgcttt ttctaaaatt tcagcatgtg gtgctggtga agcacctact
    13321 gttattttct tgtcgtcact gccacttttg tttccattac cgcaagctgc taatacaact
    13381 gcaaatgtta atactaaaat aagaccaaat aattttttca taaaatgaaa cccccaattt
    13441 atcgtttatc aagtttattt gtaagccaat ccccaatgaa ttggattata aatacaataa
    13501 ttaaaataaa aactgttgat actaaaatga catcattttg atttcgagtg aaacctgtta
    13561 agtatgctaa atttcctaaa ccaccggcac caattacacc tgcaactgct gttgaaccaa
    13621 ctaaagcgat tgctgtaact gtaatgccag acactagcgc tggcatagct tcaggtaaaa
    13681 ggactttacg aattactgtc caagtattag cgcccattga ccaagccgct tcgatgacac
    13741 ctttatcaat ttctttaaaa gcaatttcta cgagccttgc ataaaacggt gctgcgccaa
    13801 tgatcaaggc tggtaacgca cctgtcggac cacttatcgt tccaagtatc aaacttgtaa
    13861 atggaattaa taataaaatt aaaataataa atggtatcgc tctaaataag ttaacaatga
    13921 aagaaacgat agaataaaat aaccttgcac cgatagactt acctttagca gacaagaata
    13981 ataacacacc taaaataaga ccaagtataa atgcaaatat agttgagacg actgtcatgt
    14041 atagtgtttc gactattgca gtccaaactt ctggccactg aatattaggc attgtaatca
    14101 tttcatttat aatttcacta aatgatttac ccatgtctta acacctccat tttaacttgt
    14161 cgctcaatta actctttttc gaattttccg aaatctacac ttgaaatata tggaatatgc
    14221 agaactaaaa agccgactgt tccatttttt gtatttttaa tatttgcttc taaaatatta
    14281 attttaatat cataggcagt tgatagactc gatacaatag gctcggttgt tgttgaacca
    14341 gcgaaaacta atctaacgat atatgcatct ttttctaatg gctctaattc tgttaaagat
    14401 gtttcgaaat catcatttaa atcgtctttc acaaatcgtt ttgtcacagt gtgttgcgga
    14461 ttttcaaaaa cctgtgtcac cggtccttgt tctatcactt taccactttc cataactgca
    14521 acttcatcac aaatacgacg aatgacatgc atttcatgcg taattagtac aattgttaaa
    14581 ttttgttgtt ctctaatttt tagtagtaga tctaaaattt catctgttgt ttgcggatca
    14641 agtgcacttg ttgcctcatc acaaagcaag accgttggat cattagctaa cgctcgtgca
    14701 atcccaacac gttgcttttg tccacctgat aactctgatg gataagcctt ttctctacct
    14761 tttaaaccga cgagttcgac aagttctaat gctttttgct tagctctcct tctagggaca
    14821 cctgcaattt caagcggaaa cataatattt tttaacacag tccttgacca taacaaatta
    14881 aaatgttgga agatcatact tactttttgt ctttttgctc ttaatccatt tttggacaat
    14941 tgacctatat ggtctccatc tataataact tcacctgatg taggcgcttc taaatgatta
    15001 aacattcgaa tcaaagtact ttttcctgct ccagaaaaac caatgacgcc ataaatcgat
    15061 cctgctcgaa tcgataaatt aacgtgatct acagcaagga cttctttatt tttagtccga
    15121 tattctttaa caacttcttt taattcaatc acgttgattc ctccctgtgt tgcttaataa
    15181 aataaaaaat gctttctcaa tatcgataga aaaattgaga aagcaatagt agtattgttt
    15241 ctctcatctt caaaagttaa aactttatgt gaattggcac catttctata taagacggtt
    15301 gccgggcttc gtagggcaca tccctccacc actctcgata agagtttacg catcatttaa
    15361 tttgtattaa tcctaacacc ttagtaaaat ttcgtcaata actattttaa attttctaac
    15421 aaatcagtca ccgatttaaa tgcataaatt cgttttactt ctttatcttt attcatcaac
    15481 aataaaatcg gcgtagacat gatttgcata tctttacaaa actgaggata aaagtttaaa
    15541 tctattttca ataatggtaa ctgcaatatt tcattagcaa tgtctaacat tctttctgaa
    15601 accttacaag taccacacgt tggtgtataa ccaaagatta aatgtttgtc ttcctcataa
    15661 aatgtagtta catctttgat gtctaatgaa ttattcattt actaaaacta acctttcatt
    15721 atttatattc ggtaaaagag gtgtttcttt cttacaagta aagccatgtt ttgaaagtac
    15781 atgcgccaaa tattgtttgg ggcaattcgc aacttgacag taagttttat caataaatat
    15841 atgttcactt tcactcaaat aacgtttaaa ccaatttcta attcgatctc cttcgtcatc
    15901 agaatcggct aatacaaaaa cttgtttatc atacagtgat tctatcatat catcaagctt
    15961 atctatactc attgttccat gagtacaaat aatattgact ggttctgcaa taacctgttg
    16021 cacccttttt ttatcagatt ttccttcaac aattatcact ttatttacaa tagccatcat
    16081 catcaccctt taaaatcaat aaacatctgt cactgtatca tttcacaaaa ttggtatgaa
    16141 taaaacataa atcacaaaaa atttaaacta gcttaatata ataattacaa actcaatgtt
    16201 tgactagctg gaacatttaa cataagcaga caaaggctaa gtcaaaaatc aacatcctaa
    16261 aatctacaat gttatattaa caatagttaa ccaaaagaaa atacacctat aacaaacttt
    16321 tcaattatag cggggcccca acacagaagc tgatggtaag tcagcttaca ataat
//
LOCUS       DALAGF010000062        16157 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.062, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000062 DALAGF010000000
VERSION     DALAGF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16157
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.062"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..303
                     /locus_tag="O2692_001759"
     CDS             <1..303
                     /locus_tag="O2692_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HCZ4299387.1"
                     /translation="KKEKDDNILCEEFSYKCSIKDIKYKKLLKAISNQDFKGLVPNIN
                     QNDNYSEIYIINNTKNLIYHLYDDRGLWLAFNNNEDYARYSEKYNDLISEFDNEDI"
     gene            complement(396..557)
                     /locus_tag="O2692_001760"
     CDS             complement(396..557)
                     /locus_tag="O2692_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299388.1"
                     /translation="MLLILELRFWNLEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(594..1334)
                     /locus_tag="O2692_001761"
     CDS             complement(594..1334)
                     /locus_tag="O2692_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCZ4299389.1"
                     /translation="MFNLKHDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNVCHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLMTE"
     gene            1540..2331
                     /locus_tag="O2692_001762"
     CDS             1540..2331
                     /locus_tag="O2692_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCZ4299390.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            2699..3127
                     /gene="lacA"
                     /locus_tag="O2692_001763"
     CDS             2699..3127
                     /gene="lacA"
                     /locus_tag="O2692_001763"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCZ4299391.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            3143..3658
                     /gene="lacB"
                     /locus_tag="O2692_001764"
     CDS             3143..3658
                     /gene="lacB"
                     /locus_tag="O2692_001764"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCZ4299392.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            3671..4603
                     /gene="lacC"
                     /locus_tag="O2692_001765"
     CDS             3671..4603
                     /gene="lacC"
                     /locus_tag="O2692_001765"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCZ4299393.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            4607..5587
                     /gene="lacD"
                     /locus_tag="O2692_001766"
     CDS             4607..5587
                     /gene="lacD"
                     /locus_tag="O2692_001766"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCZ4299394.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            5609..5920
                     /locus_tag="O2692_001767"
     CDS             5609..5920
                     /locus_tag="O2692_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCZ4299395.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            5926..7638
                     /locus_tag="O2692_001768"
     CDS             5926..7638
                     /locus_tag="O2692_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCZ4299396.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            7656..9068
                     /gene="lacG"
                     /locus_tag="O2692_001769"
     CDS             7656..9068
                     /gene="lacG"
                     /locus_tag="O2692_001769"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCZ4299397.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIIDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            9334..10203
                     /locus_tag="O2692_001770"
     CDS             9334..10203
                     /locus_tag="O2692_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4299398.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIIMVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRRDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            10486..11487
                     /locus_tag="O2692_001771"
     CDS             10486..11487
                     /locus_tag="O2692_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCZ4299399.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            11721..12722
                     /locus_tag="O2692_001772"
     CDS             11721..12722
                     /locus_tag="O2692_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCZ4299400.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISTNPAENEGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEIPLVDYIF
                     CTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTP
                     DMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVINI"
     gene            13029..14591
                     /locus_tag="O2692_001773"
     CDS             13029..14591
                     /locus_tag="O2692_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCZ4299401.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPTGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLIKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
     gene            14766..15302
                     /gene="amaP"
                     /locus_tag="O2692_001774"
     CDS             14766..15302
                     /gene="amaP"
                     /locus_tag="O2692_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCZ4299402.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            15315..15554
                     /locus_tag="O2692_001775"
     CDS             15315..15554
                     /locus_tag="O2692_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCZ4299403.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            15617..16126
                     /locus_tag="O2692_001776"
     CDS             15617..16126
                     /locus_tag="O2692_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCZ4299404.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
BASE COUNT         5718 a         2362 c         3071 g         5006 t
ORIGIN      
        1 aaaaaggaga aggatgataa tattttgtgc gaggagtttt catataagtg ttccataaaa
       61 gatataaaat ataaaaaatt attaaaagct atatccaatc aagactttaa agggttagtt
      121 ccaaatatca atcagaatga taattattct gaaatatata taattaacaa tactaaaaac
      181 ttaatatatc atttatatga tgatagaggt ctatggctag cttttaataa taatgaagat
      241 tatgcaagat attctgaaaa atataatgat ttaatatcgg aatttgataa tgaagatatc
      301 taatttttta atattcgtag cttttatttt tctacttatt ttatgtttat ttttaatctt
      361 acaaatgaca aaccattaaa agtagcatcc caacatcaaa caaaaaaaca attcatcaaa
      421 taaaaatcgc tacaaaacca agtcattaaa cacgcaataa ttaaaatttt ccactcatta
      481 taattctgaa ttccaaatgt cgaattccga aaaccaaact ccaaattcca aaaacgcaac
      541 tccaaaatta aaagcatttc cctaccattc gggaaatgct ttttacatac tgattactct
      601 gtcattaatg attttacaac ggaaaccatg tcgtcatgta tgaccaaagt agcgtcgcta
      661 tcataaggtg ttcgatcttt attgataata attaaattgt cgcctttaaa atgtgatatt
      721 aatcctgcgg caggttgtac aacgagtgat gaacctagta caacaagggt gtcagcatgt
      781 tcaattttat ttaatgccct tatgatggta ggttgatcta acatttcacc gtataatacg
      841 atgtccggtc gaatggcacc accacaatta tcacaatgtt tcaaagttct atcaataaca
      901 tctgacttcg tataagattt atgacataca ttacaataaa aacgatttaa cgtgccatgt
      961 aattcatcaa catgttgact tccagcgtct gagtgcaaac catcgatatt ttgcgtgatg
     1021 acacctaaag attgttgatt acgttctaat tttgcaatcc aatcatgaac gatattgggc
     1081 atcgtatcga caaatagtaa gcgcttatgg cagaaattga taaaaccttc aggatcatct
     1141 tctaaataat cacggcttaa caagtattct ggcgaaagcc catcttttga aatttcatca
     1201 aataagccac ccattgaacg gaaatctgga acgccacttg cgacagatac accagcacct
     1261 gtaaaaaatg taatacgatt cgaactatct ataatatgtt ttagtgtctc taaatcatgt
     1321 ttcaagttaa acaccccatc aaaataatga atgatttata acgtctttta cttttattat
     1381 agtatattca ttttacgatt atcaaaagcg cagtttaaac agttcttttt aaaaatgaat
     1441 tatacatata atcgaacagt taaattatat tagtttttac gcaattttta ttatagaata
     1501 agcatgtata ttaaaattga tgtctaaaca taaaggataa tgacccatcg cctaaataaa
     1561 ggaggcaatt ttacattgaa taaacatgaa cgtttggacg aaattgctaa actagtgaat
     1621 aaaaagggca cgataagaac gaatgaaatc gtcgaaggtt taaatgtgtc tgatatgaca
     1681 gttcgaagag atttaattga attggaaaat aaagggattt taacgaagat tcatggtggt
     1741 gcacgcagta attcaacatt tcagtataaa gaaatatcgc ataaagaaaa acatacccgt
     1801 caaattgcag aaaaacgatt tatagctaaa aaagctgcat cattaattga agatggggat
     1861 actttgtttt tcggaccagg aacaacagtg gaactattag cagaagaagt caatcatcat
     1921 acgctcacaa ttattacgaa ttgtttgccg gtgtataaaa ttttgttaga aaaacaaaca
     1981 gcacattttc gtgtctattt aattggtggt gaaatgcgcc atattacaga agcatttgta
     2041 ggtgaaatgg cgaacgccat gttggaaaaa ctaagattta gcaagatgtt ctttagtagt
     2101 aacgcagtaa ataaaggtgc tgtgatgaca tctacattag atgaagccta tacgcaacaa
     2161 ctggcactaa gtaattcaat tgaaaaatac ttgttaatcg atcatacgaa agttggcaaa
     2221 gaagatttta catcattttg tcagttaaac gaattgactg cagtggtcat ggactatgaa
     2281 gatgaagaaa aagtagaaac gattaaaaca tatattgaag tagtcgacta aattaaaagt
     2341 ataactgcat tgttaaaata tgtatctata tgctcataag tgaatgtgaa tttgcattca
     2401 tttatgggct attttttatg caaatttatg tcaacgggaa cctcgacaaa gttttactac
     2461 aatttcagac gaatacctaa ttaaaacaac accacaatat gaatgttcac ttattgtttg
     2521 aaaatacgtc ataaacaaac atattataaa catattatgt tgatttattg tttgtttatg
     2581 tttataattt aaacgtaatc aaattgaaag ccttttctca agattttcat aacctacaaa
     2641 taaatatgtc acattttaag aaaggtattt caaaattaaa gtaaaaagga gtcttattat
     2701 ggcgattatt attggttcag atgaagctgg caaacgatta aaagaagtca tcaaatcata
     2761 cttattagac aataaatatg atgttgttga cgtaacagaa ggacaggaag ttgactttgt
     2821 tgatgcaact ttggctgtag caaaagatgt tcaaagtcaa gaaggtaact taggtattgt
     2881 tatcgatgca tttggtgcag gtagctttat ggttgctaca aaaatcaaag gcatgattgc
     2941 tgctgaagta tcagatgaac gctctggata tatgactaga ggccataaca attcaagaat
     3001 gatcactatg ggatctgaaa ttgttggaga cacattagcg aaaaatgtcg ttaaaggttt
     3061 tgttgaaggt aaatacgatg gtggtagaca ccaaatccgc gtagatatgc ttaataaaat
     3121 gtgctaagga ggaaaaatta ctatgaagat tgcattagga tgcgaccata ttgttacaga
     3181 tacaaaaatg cgtgtatctg aatttttaaa atcaaaagga catgaagtca ttgacgtagg
     3241 aacatacgat ttcacaagaa cacattatcc aatttttggt aaaaaagttg gcgaacaagt
     3301 tgttagcggt aatgcagact taggtgtttg tatttgtgga acaggtgttg gtattaacaa
     3361 tgctgtaaat aaagtacctg gcgttcgttc agcactagta cgtgatatga catcagcgtt
     3421 atacgctaaa gaagaattaa atgcgaacgt tattggcttc ggtggacgta ttataggtga
     3481 gttattaatg tgcgatatta tcgatgcatt tattaatgct gaatataaac caactgaaga
     3541 gaacaaaaaa ttaatcgcta aaattaaaca tttagaaaca agcaatgcag atcaagctga
     3601 tccacatttc tttgatgaat tcttagaaaa atgggacaga ggcgaatacc acgattaagg
     3661 gacgacacat atgattttaa ctttaacatt aaatccatcc gttgatattt catacccatt
     3721 aacagcattg aaattagatg atgttaatcg tgttcaagaa gtaagtaaaa cagctggtgg
     3781 taaaggttta aatgtaacaa gagtacttgc gcaagtaggt gagccagtcc ttgcgagtgg
     3841 ttttattggc ggtgaattag gtcaatttat tgctaaaaaa ttagatcatg ccgacatcaa
     3901 gcatgcattt tataacatta agggtgaaac acgaaactgc attgcaattt tacatgaagg
     3961 gcaacaaaca gaaattttag agcagggacc ggagattgat aatcaagagg ctgcaggatt
     4021 tattaaacat tttgaacaat tattagaaaa agttgaagca gttgctattt caggtagctt
     4081 acctaaagga ttaaatcaag attattatgc acaaatcatt gaacgatgcc aaaacaaagg
     4141 tgtgcctgtg atattagatt gctcgggtgc aactttgcaa acagttttgg aaaatccata
     4201 caaacctaca gtcattaaac caaacatttc tgaactatat caattgctta accagccact
     4261 tgatgaaagt ttagaaagtt tgaaacaggc agtgtcacaa cccttattcg aagggattga
     4321 gtggattatt gtgtctttag gtgcacaagg tgcattcgct aagcacaatc atacattcta
     4381 tagagtgaat attccgacaa taagtgtatt aaatccggtt ggttcaggtg attctacagt
     4441 tgctggaatt acttcagcaa ttcttaatca tgaaaacgac cacgatttac taaaaaaagc
     4501 caatacttta ggcatgctga atgcacaaga agcacaaaca ggttatgtca atttaaataa
     4561 ttatgatgat ttattcaatc aaattgaagt tttagaggtg taaaaaatgt caaaatcgaa
     4621 tcaaaaaatc gcatcaattg aacaattaag taacaacgaa ggtatcattt ctgcattagc
     4681 atttgaccaa cgtggtgctt taaaacgcat gatggcaaaa catcaaactg aagaaccaac
     4741 tgtagctcaa attgaacaat taaaagtatt agttgcggaa gaattaacac aatatgcatc
     4801 ttcaatttta ttagatcctg aatatggttt accagcttca gatgcacgta acaaagattg
     4861 tggattatta cttgcttatg aaaaaactgg atatgacgta aacgctaaag gtcgtttacc
     4921 tgactgttta gtagagtggt ctgcaaaacg tttaaaagaa caaggcgcaa atgctgttaa
     4981 attcttactt tactatgatg ttgatgatgc tgaagaaatt aacattcaaa agaaagcata
     5041 cattgaaaga atcggttcag aatgtgttgc agaagatatt ccattctttt tagaagtatt
     5101 aacatacgat gacaacattc ctgataacgg tagtgttgaa ttcgcaaaag ttaagcctag
     5161 aaaggttaac gaagcaatga aattattctc agaaccacgc tttaacgttg atgtattgaa
     5221 agttgaagta ccagtaaaca tgaaatatgt agaaggcttt gctgaaggtg aagtggttta
     5281 tacaaaagag gaagcggcac aacactttaa agatcaagat gcagcaactc atttaccata
     5341 catttactta agtgctggtg tttctgcgga attattccaa gaaactttaa aatttgctca
     5401 cgaagctggt gctaaattca acggggtact ttgtggccgt gctacatggt ctggtgctgt
     5461 tcaagtatac attgaacaag gtgaagatgc tgctcgtgaa tggttacgta caactggttt
     5521 caaaaatatt gatgacttaa ataaagtatt aaaagatact gcgacatctt ggaaacaaag
     5581 aaaataagtt taaaagggga ttaatgctat gaatagagaa gaagtccaat tattaggttt
     5641 tgaaattgtt gcatttgcag gggatgcacg ttctaagttt ttagaagcat taacagcagc
     5701 tcaagctgga gattttgcaa aagcagatgc actgattgaa gaaggaaaca attgcattgc
     5761 ggaagcgcat agagcacaaa caagcctgtt ggctaaagaa gcgcaaggtg atgatattgc
     5821 atacagtgta acgatgatgc atggtcaaga tcatttaatg acaacaattt tactgaaaga
     5881 tttaatgaag catttattag aattttataa aagagggtga tgacaatgat gcaaaaattg
     5941 atcgcacaaa ttgaaaaagg gaagccattt ttcgagaaat tatcacgtaa catatattta
     6001 agagccatcc gtgatggatt tatttctgca atgcctgtca tcttattctc aagtatcttt
     6061 ctattaattg catatgtgcc aaatatattt ggttttaaat gggataaagg catggaagcc
     6121 attttaatga aaccatacaa ctatacgatg gggcttgtag catttttagt agcaggaacg
     6181 acagcgaaat ctttaacaga ttcatttaac cgtaagctag aaagtacaaa tcaaattaac
     6241 tttatttcta caatgctagc agcgatgtgt ggattcttgt tcttagcttc tgatccagcc
     6301 aaagatggtg gattcttaag tgcatttatg ggaactaaag gtttattaac agcattctta
     6361 tcagcatttg taactgttat tgtttataac ttctgtgtga agcgcaatat tacaattaaa
     6421 atgccgaaag aagtaccgcc gaatatttca caagtattta aggacttaat tccattttca
     6481 gcggtaatca tcattcttta tgcattagat ttagtcattc gcaacagctt taaatcaaat
     6541 gtagcggaag gtattttaaa attattcgaa ccattattta cagcagcaga tggatggatt
     6601 ggtgtcacaa ttatctttgg tgcctttgca ttattctggt ttgtaggtat tcatggtccg
     6661 tcaattgtag agccagcaat tgcagccatt acatatgcga atatcgaagc gaacttcaag
     6721 ttgcttcaag ctggagaaca cgcagataaa attattacat caggtacaca aatgtttatc
     6781 gttacatttg gaggtacggg tgcaacattg gtcgtcccgt tcatgtttat gtggatgacg
     6841 aaatctaaac gaaacaaagc gattggacgt gcatcagttg taccaacatt ctttggtgta
     6901 aacgaaccaa ttttatttgg tgcaccacta gtattgaatc ctgtgttctt tattccattt
     6961 gtattagcac caattgttaa cgtatggatt ttcaaacttt tcgttgaagt gttaggaatg
     7021 aatagtttta gtgtgaattt accttggaca acaccaggtc cattgggcat tatcatgggt
     7081 acaggttttg gtttatggtc attcgtacta gctattactt tgattgttgt agatattatt
     7141 atttactacc cattcctaaa agtttatgat agtgaaattc ttgatgaaga agaaggacgt
     7201 aaagaaagta attcagattt aaaagaaaaa gttgcagcaa actttgatac gaaaaaagct
     7261 gattcaattt tagcggcaag tggtgtatca gacgatgcag ctaaagcttc aaatatcaca
     7321 gaacaaacaa atgtcttagt actttgtgcc ggtggtggta caagtggatt attagccaat
     7381 gcacttaata aagcagctga agaatatcat gtgccagtga aagcggcagc tggcggatat
     7441 ggcgcacata tggacattat gaaagagtac caattaatca ttttagcgcc acaagttgcg
     7501 tctaactatg aagatattaa gcaagataca gatagattag gtattaaatt agcgaagact
     7561 caaggtgctg aatatatcaa attgacacga gatggccaag cagcccttga tttcgttcaa
     7621 caacaatttg aaaattaaga ataggagttt ttcatatgac taaaacatta cctgaagatt
     7681 ttatttttgg tggagcaaca gcagcatatc aagcagaagg tgcgacaaat acggatggta
     7741 aaggtcgcgt agcatgggat acgtatttag aagaaaacta ttggtacact gcagaaccag
     7801 cgagtgattt ttataacaga tatccagtgg atttagaatt aagtgaaaaa ttcggtgtta
     7861 atggtatccg aatttcaatt gcatggtctc gtatcttccc aaatggatat ggcgaagtta
     7921 accctaaagg tgttgagtat taccataagt tatttgcaga gtgtcataaa cgtcatgttg
     7981 aaccatttgt aacattgcat cattttgata caccagaagt gttacacaaa gatggcgatt
     8041 ttctaaatcg taaaacgatt gactactttg tagattatgc tgaatattgt tttaaagaat
     8101 tcccagaagt aaaatattgg actacattca atgaaattgg acctattggt gatggtcaat
     8161 atttagtagg taaattccct ccaggtatta aatatgattt tgaaaaagta ttccaatcac
     8221 atcataacat gatggtggct catgctagag cagtaaaatt atttaaagat ggcggataca
     8281 aaggagaaat tggtgttgta cacgcattgc caacaaaata tccgttcgat ccatcaaatc
     8341 ctgaagatgt tcgtgcagca gaattagaag atatcatcca taataaattc atcttagatg
     8401 caacatattt aggtaagtat tctcgtgaaa cgatggaagg tgtgcaacat atcttatctg
     8461 ttaatggcgg taaattaaac attacagatg aagattatgc cattttagat gcagccaaag
     8521 atttaaacga cttcttaggt atcaattact acatgagtga ttggatgaga ggttacgatg
     8581 gagaatctga aatcacgcat aatgcgacag gtgataaagg cggttctaaa taccaactta
     8641 aaggtgtcgg acaacgtgaa tttgatgtcg acgtacctcg cacagactgg gactggatga
     8701 tctatcctca aggtttatat gatcaaatca tgcgtgttgt taaagattat cctaactatc
     8761 ataagattta tatcactgaa aatggtttag gatataaaga tgaatttatt gaatctgaaa
     8821 aaacagttca tgatgatgca cgtattgatt atgtaagaca acatttgaac gttattgcag
     8881 atgctattat agatggtgca aatgttaaag gttactttat ttggtcatta atggatgtgt
     8941 tctcttggtc aaatggttat gaaaaacgat atggtttatt ctatgtcgat tttgagactc
     9001 aagaacgtta tcctaagaaa agtgcttatt ggtacaaaga gttagccgaa acaaaagaaa
     9061 ttaaataatt atagatatta aaaggcctat cagtcgagtg ctggtaggtc tttatttata
     9121 gaaagagata aattaaacat gcatatacat atttatatat taatcaatgg gtgacacacc
     9181 attgaaagct aaagaagcaa tcaattaaca aacgttaacg gacaactttc caaagtcatc
     9241 ttaaaagtta atgatgtatt caaaatcaac taaaggacat ttttaatcac atcctttata
     9301 cttatataaa agacatgaat gggagttaaa agtgtgaaga aaatttttgt aattattacg
     9361 acattgttag cggtcgctat tattataggc tctattatta tggtggtgtt tagtcaacga
     9421 caggcacaaa catttaaaat acaacaacag caatttgtta aaaagccaat tccaacattg
     9481 ttcttacatg gatttggtgg tagtgccaat tctgaaaagt ttatggtgaa gcaagcagaa
     9541 aaaagagggg taactaaaga tatcatcact gcttacgttt caaaggatgg tgcagtgaca
     9601 tttaaaggga aactaagaag agatgcagtg aatcctattg tgaaaattga acttgaaaat
     9661 aacagacaag gctatttgga taagaatgct gcgtggttta aaaatgtact gactaagtta
     9721 caatctgaat acaatttcga taaattcaac tttgtcggtc actcaatggg gaacttaaca
     9781 tttgcacaat atatgatgac atatggtaac gacaagtcac tgccacagtt aaacaaacaa
     9841 gtgaatattg cagggacatt taatggtgtt ttaaatatga atgaagatgt caatgaaatt
     9901 acagttgata aagatggtaa accaagtcga atgaatcaac cttatcagca attacgcgtg
     9961 ctaaaagata tttataaagg taaaggtatc gaagtgctaa atatttatgg ggatttgaaa
    10021 gatggtacac attcagacgg cagagtgtct aatagttctt cgaaatcatt aaaatattta
    10081 ctgggaaata gtccgaaaag ttatcgagaa tctaaatatg aaggtgagcc tgcacaacat
    10141 agtcagcttc atgagaatga aaatgttgct aatgagttaa tcgacttctt atggaaaaaa
    10201 taaagcaact gctagttttt ctggggcaaa aaagcttgta atattagagc ttttcaaaaa
    10261 ttatctattg tttgagagtt tccataaaat gtctaagtcg taacatttta aatttatgat
    10321 tatagcaaat ataatgtggt tgaagttaca tgaatgaatc agtgcttaag tcaaaatata
    10381 acaactaaga atgtatttgc atacagacat cacgaagaca caattcaaac ttaacgctta
    10441 agtaacaaat aagtaaaagt ttcaaataaa gaaaggtgat taatgatgca aaataaacaa
    10501 attcttttta acaaaatacc agaaggcatg cctcaagagg atacattcaa aattgaagag
    10561 attgacacgc ctaaacttga gagcgatggt gtacttgtac aaacgctgta tatttcagtg
    10621 gatccatata tgagaggacg tatgactaag gcagattcgt atgtacagcc ttttgaaatt
    10681 ggtaaaccga ttgttagcca tgtcgttgca aaagtaattg attcgacact cgcagattat
    10741 aaaaaaggag atgtcgttgt aggtatgttg ccttggcgaa tcattaatca tgtccaagcg
    10801 gatcaaatta ctaaagttcc aacgacagat gtaccacttg atttatattt aagtgtttta
    10861 ggaatgccag gacaaacagc atatcatggt ctgcttgata ttggacaacc taaagccggt
    10921 gatactgttg tggtttcagc tgcatctggt gctgtaggtt cagttgtagg tcaaattgct
    10981 aagattaaag gttgccgtgt agtaggtatt gcgggtggcg acaaaaaagt aaattattta
    11041 acagagacac tgggctttga cgctggtatt gattataaga aagaggattt tgcagaagcg
    11101 ttagcacaag cagtaccaga tggtattgat gtttattttg aaaatgttgg tggaacagtt
    11161 ggcgatgaag tatttaagca tttaaatcgt tttgcacgtg taccagtatg tggcgcaatt
    11221 tcgtcatata atcatcctga agccgatatc ggaccacgta ttcaaggtac gcttattaag
    11281 aagcaagtaa tgatgagagg cttcttggta agtgaattcg ctaatgcttt taaagaagct
    11341 agtgaacagc ttgcaacatg ggttcaagaa ggtaaaattc agtcacaagt aaccattgaa
    11401 gatggttttg aaaatgcgcc acatgcattt aaaaatttat ttactggcga taactttggt
    11461 aaacaagtta ttaaagtaac ggaataataa agtatacgtt ttaacaaact aactttgtta
    11521 attgaccaaa atttaataat tttttatcta aagtaaattt gttcagcaac atggtagaaa
    11581 taagtctaaa atttaatacg aaagtcgtct gtaattattt atatattcaa atgaaaaagg
    11641 agtcatgatg ctgcgatata agcacacatg gcttctattt tgtataataa gaaataagtt
    11701 cataaatgag gtgaaacgat atgaaaatga taggatttga gaagcccttt aaattggaag
    11761 aaggaaattt atttaaagta tacgaacaaa gaaagccaac gccagaaaac gatgatatct
    11821 tagtcaaagt aaatagcatt agtgtaaatc ctgtagatac aaagcaacga caaatggaag
    11881 tcactcaagc acctcgtgta cttgggtttg atgctattgg tactgttgaa gctatcggac
    11941 cagacgttac attatttagt ccgggtgacg ttgtatttta tgctggatcg ccaaatagac
    12001 aaggatcaaa tgcgacatat caattggtgt cagaagctat agttgcaaaa gcaccacaca
    12061 acataagtgc taatgaagcg gttagtctac cgctaactgg tattacggca tatgaaacgt
    12121 tttttgatac atttaaaatt tctacgaatc ctgctgaaaa cgaagggaag tctgtcttaa
    12181 tcattaatgg tgccggtggt gtaggcagta tagccactca aattgctaaa cggtatggtt
    12241 taactgtcat tacaacagca tcacgacaag aaacaacaga gtggtgtgaa aagatgggcg
    12301 cagatatcgt attgaatcat aaagaggatt tagtgcgtca atttaaagaa attccattag
    12361 tagattatat attttgcacc tataatacag acttgtatta caatactatg attgaactga
    12421 taaaaccatt agggcatatt acgactatcg tagcttttaa tgaagatcaa gatttaaatg
    12481 cattaaaatt aaaaagtata acatttacac atgaatttat gtttgcgcgt ccaattcatc
    12541 gaacgcctga tatgattaaa caacatgagt atttagaaga tattacgaaa aatatagaat
    12601 cagggcatta ccaaccaaca acgacacaag tttttgaagg tttatcacct gaaaacttat
    12661 atcaagcaca tcaattgttg gaaaaacaat caatgattgg aaaattagta attaatatat
    12721 agattagtaa gtaaagtcgc cttatgcaga aaatactgta taggcgactt attttttgtg
    12781 tctagcgact attgattcta aacggaaata tggcaacttt gcaggtatca aatcaataat
    12841 gcactagaaa ggcattactt aaatacaata aaatactttg ttgctcaatg ataaataatt
    12901 tgagtgtgtt ttatatatca tttttaaaag atgaggcatt attaattgtg tctatataaa
    12961 attacaaatg attaaaatgt tgcagttatg ggtatgaact taccattaca cagaaagggt
    13021 ttgtatttat gaaaaataaa tcagtaagtc tagtgttttg ggtatcttta gtgatatgta
    13081 cgatatttgt tgcttttgga gcaatatttc cgaaacagtt agaaaaactc acgcaaaaca
    13141 ttactacatt tattgcgcta catttttctt ggtattattt attgctcgtt ttagttattt
    13201 tatttgtctg tgtgtatatt ttattttcga gatacgcaaa cattacatta ggtgaagaag
    13261 gcgaagatcc tgaattctca ttgccgtctt ggtttgctat gttatttagt gctggtatgg
    13321 gaataggact tgtgttctgg acaacggcag aaccaatcag tcatgccttc aaattaacgc
    13381 ctattcataa agctggcacg caatctgcaa ttaatgatgc aatgcagttt tcatttttcc
    13441 attgggggat tcatgcatgg gcagtctatg gtattgtcgc gctagtgttt gcatatttta
    13501 gctttcataa aggatatcct ggcttagtca gcgctacatt gacgccttta ttaggtgaaa
    13561 aggcaatgcg tggaccttta ggtggtgcca ttgatgtatt ggcagtcata gctacagtaa
    13621 caggcgttgc tgcaacatta ggtttcggtg cattgcaaat aaacgaaggt ttacattttt
    13681 tatttaatgt accgagtaac ttcacgatgc aagtaatctt gattgtcatt gcaaccatat
    13741 tatttacttg gtcagcatgg tcaggtatcg ataaaggtat caaaacgttg agtaatatca
    13801 acatgttact tgcttttgta gtacttattg gtctttttat tgttgggcca acgctataca
    13861 ttttaaatac atttacgaat ggtctaggta attatattgc aaatttcttt agtatgagtt
    13921 tacgcattcc gacaggtggt caaaaattcc aatggttaca aaattggaca atcttttact
    13981 gggcatggtg gatatcttgg gcaccatttg taggtatatt tattgcacgt gtgtcaaaag
    14041 gacgtacgat taaagaattt attctaggtg tactttttgt cccggcgtta gtttgcttta
    14101 tattctttgc ggtattcggc gcgtcagcaa tttacttaca agataatcat attgccgata
    14161 ttgctaaagc tgcgacagaa acagcaactt ttgcgacgct acaacattat ccgctaggtt
    14221 ttgtattaag cttaattact ttacttgtta ttatgatttt ctttgttaca tcagcggatt
    14281 cggcaactta tgttttaggt atgttgagtt caagtggtga tattaatccg aagtcatttg
    14341 tcaaagtgag ttggggtatc attatggcat tatttgcaat catcatgatt tacacaggtg
    14401 gtactcaagc aattcaaaat ttattaatca ttgctgcgtt accattttcc gtggtcatta
    14461 tagcgatgat atggtcgctt ataaaatctt tgagtgagga gaaaccgaga aatagtaata
    14521 aggttttaat taaacatcgt gacccagata ttttagaata tcgttcttca aaacatattg
    14581 acgaaaatta attaataatg aggttttatt tacaaaaact agggaatata ttactaaatg
    14641 gtacttcatc ttatatatta aaaataaatt taaagttatt aatttattat gtttaagaaa
    14701 aaaactgatg ggtacatatt taatatgttt ttaaattaac tttaactaag gaggtgtcgt
    14761 taggggtgaa acgacttaaa aactttatcc ttggcttatt aatagtagta atcgtggggt
    14821 tcctgttatt catgtatatc caagatggac gtataactga atatcaagac tacttccttc
    14881 aatttgaatg gttccaacca ttacttattt cacttgctgc actattgata ttaataggtc
    14941 ttatattagt atttagtatc ttcaaaccta cgcatcgaaa acctggatta tacaaagact
    15001 tcgatgatgg tcatgtttac gtatctcgta aagctgttga aaagacggca ttcgatacgg
    15061 ttgcaaaata tgatcaagta agacaaccaa atgtagttgc gaaactatat aacaaaaaaa
    15121 ataaatctta tatcgacatt aaaactgact ttttcgtacc aaataatgtt caagttcaat
    15181 ctttaactga agcaattcgt tcggatatta aaaagaatgt agaatatttc acagaaatgc
    15241 cagtacgtaa gttagaagtt aatgtgagag atcagaaaac ttctggtcca cgagtgttgt
    15301 aagggaggta tatcatggct aacaatcata accaaaacgg acaagactct acacaacaag
    15361 tgattaattt tttaaaagta tttaaatgga gaatcgttgg tttcctagcg tttctattaa
    15421 tcgcgatatt attcttaacg ttaggttttt ggaaaacagt acttatcatc gttttatgtt
    15481 taattggtgt aggtattggg tatatgaaag accgtaagca agattttatg aattttttaa
    15541 atcgatggag ttaattcatc aatttagaat ttaattaaaa aaatataaaa attaacaagt
    15601 aaaaggagaa tctattatga ctgtagataa caataaagca aaacaagcat acgacaatca
    15661 aactggtgtt aacgaaaaag aaagagaaga gcgtcaaaaa caacaagaac aaaatcagga
    15721 gcctcaattc aaaaacaaat taacattctc tgatgaagtt gttgaaaaaa ttgctggtat
    15781 cgctgcacgt gaagttaaag gtatcttaga tatgaaaggt ggcttaactg atacattcac
    15841 taatgcattc tcaagtggca acaatgttac tcaaggtgta tctgttgaag ttggtgaaaa
    15901 acaagctgct gtagacttaa aagtaatctt agaatatggt gaatcagcac caaaaatctt
    15961 ccgtaaagta actgaattag ttaaagaaca agttaaatat attactggtt tagatgtagt
    16021 tgaagttaac atgcaagttg acgatgtaat gactcaaaaa gagtggaaac aaaaacacga
    16081 aaaaaataac gaaaacaata accaagaaag acaaggttta caataagtta ttcgataaaa
    16141 agggcttggg acattaa
//
LOCUS       DALAGF010000063        16108 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.063, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000063 DALAGF010000000
VERSION     DALAGF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16108
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.063"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(98..1147)
                     /locus_tag="O2692_001777"
     CDS             complement(98..1147)
                     /locus_tag="O2692_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HCZ4299405.1"
                     /translation="MIPTLEEIIDKYGNLVDYLKFDVTVEVYEDLLLLRSLSDLNEHQ
                     KMDRVSFIKKHLNSNFDYLLDDTEFRSINKIYSNLNIMTHINSQNNNFKRNPFINEEQ
                     LKSLLAIKKVDAHMSYDSNITSKALAEINKTQKRLVTKIDTLYNQSKKENEYVRLGEK
                     ISYTKLENHWKYLYNEIQFYNLNNQLISYVALEQEYAWAFLNELFYLIELYLKAFKGQ
                     KKTDYEFGKKLNEFIQSYVIILLIDIRLPVVRLYIIRDIVDTCEGEKDNYKRITLIEE
                     SIKKYRYVKDQIKRFKNGLEESLLNATLSIEQNMLKVKIDYYKAYCFPREKTKHNNIE
                     YNVSLFFKALDALKK"
     gene            1285..1575
                     /locus_tag="O2692_001778"
     CDS             1285..1575
                     /locus_tag="O2692_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299406.1"
                     /translation="MNKKSKQQEKLYNFIIAKSFQQPVGSTFTYGELRKKYNVVCSTN
                     DQREVGRRFAYWIKYTPGLPFKIVGTKNGSLLYQKIGINPCNNSTPSKGGDC"
     gene            1575..3362
                     /locus_tag="O2692_001779"
     CDS             1575..3362
                     /locus_tag="O2692_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCZ4299407.1"
                     /translation="MSYTLFEIGPFALDSFGWKETIPPKKDGDSEKVVRMASPIIVNA
                     RFSDPITGVEKLIITNNNGKKDIFESDILTTRNLPSLIKYGYSINEKYIRSLSYALQL
                     MRDRLPLSELYEGVGILETPFGYLISLDKVLKSIQFNQSSPSYPIVDSAYDLTPKGTF
                     DNWFNMYIDEVKGHLLLELAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKST
                     AAALAVSVAGNPTKGNETLFRSWNATRNALEGYLSNNYGIPIVFDELSSTTLRDTTGL
                     LYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQF
                     TTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDETNNT
                     GIRMFKRYAVITTSAKILGRVLSTDIDIANIRDYFIDYHTHTVSERSLADKAIDVIIQ
                     FVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNVVN
                     ALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLLQNW
                     TPSNDNKAAKELFKSANEGIGPSGVHEDF"
     gene            3596..4945
                     /locus_tag="O2692_001780"
     CDS             3596..4945
                     /locus_tag="O2692_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ4299408.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
                     NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLT
                     NMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCRDFYQNQRIYTKI
                     KSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHIIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA"
     gene            4967..6595
                     /locus_tag="O2692_001781"
     CDS             4967..6595
                     /locus_tag="O2692_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ4299409.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
     gene            7117..7467
                     /locus_tag="O2692_001782"
     CDS             7117..7467
                     /locus_tag="O2692_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299410.1"
                     /translation="MKTITQELKQYITHLFQLSNNEAWECEALEEAAENILPERFIND
                     SPLVHLTLETYTYYNDELHELSIYPFLMYANNQLISVGYLDHFDMDFLYLTDTQNIII
                     DERHLLKQGGQDHE"
     gene            7554..7865
                     /locus_tag="O2692_001783"
     CDS             7554..7865
                     /locus_tag="O2692_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCZ4299411.1"
                     /translation="MKINRYITRGINESIPLDLQILLWHMVEKKDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPTYKLEYHYTNYEKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            7880..8386
                     /locus_tag="O2692_001784"
     CDS             7880..8386
                     /locus_tag="O2692_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCZ4299412.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKAACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIRTPENLKHIKNPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEIMHPLNPKA
                     RQKWILKS"
     gene            8438..10045
                     /locus_tag="O2692_001785"
                     /pseudo
     CDS             8438..10045
                     /locus_tag="O2692_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(10036..10296)
                     /locus_tag="O2692_001786"
     CDS             complement(10036..10296)
                     /locus_tag="O2692_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCZ4299413.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(10268..11254)
                     /locus_tag="O2692_001787"
     CDS             complement(10268..11254)
                     /locus_tag="O2692_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCZ4299414.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            11351..13360
                     /gene="mecA"
                     /locus_tag="O2692_001788"
     CDS             11351..13360
                     /gene="mecA"
                     /locus_tag="O2692_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCZ4299415.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(13406..13834)
                     /locus_tag="O2692_001789"
     CDS             complement(13406..13834)
                     /locus_tag="O2692_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCZ4299416.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(13931..14674)
                     /locus_tag="O2692_001790"
     CDS             complement(13931..14674)
                     /locus_tag="O2692_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCZ4299417.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            complement(15511..15882)
                     /locus_tag="O2692_001791"
                     /pseudo
     CDS             complement(15511..15882)
                     /locus_tag="O2692_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
     gene            15936..>16108
                     /locus_tag="O2692_001792"
     CDS             15936..>16108
                     /locus_tag="O2692_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCZ4299418.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYTLSYRDISEILRERGVNVHH
                     STVYRWVQEYAPI"
BASE COUNT         5864 a         2695 c         2447 g         5102 t
ORIGIN      
        1 cacaacattt ttcgtatttc agaacgatta agactacatt atcctggctt aatattaatt
       61 aattttaatt atatctataa atattttcag tgaaaaacta tttctttaag gcatctaatg
      121 ctttaaaaaa caacgataca ttgtactcaa tattgttatg ctttgttttt tctctaggaa
      181 agcagtaggc tttataataa tcaattttaa ctttcaacat attttgttca atagataaag
      241 ttgcatttaa taacgattct tctaatccat ttttaaatct tttaatctga tcttttacgt
      301 atctatactt tttaatagat tcttctatca gtgtaattct tttgtaatta tctttttctc
      361 cttcacatgt atctacaatg tctctaataa tatataatct aacaacaggt aatcgtatat
      421 ctataagtaa aataatgaca tatgattgaa taaattcgtt taattttttc ccaaactcat
      481 aatctgtttt cttttgtcct ttaaatgcct tcaaatacaa ttctatcaaa taaaatagtt
      541 catttaaaaa agcccaagca tattcctgtt ctaatgcaac ataactaatc aattgattat
      601 taagattata aaactgaatc tcattatata aatatttcca atgattctcc aattttgtat
      661 acgatatttt ttctcctaac cgtacatatt cattttcttt ttttgactga ttatagagtg
      721 tgtctatctt tgtaactaat cttttttgcg ttttgtttat ttctgccaat gcttttgaag
      781 taatatttga atcataactc atatgtgcat ctactttctt tatcgcgagt agtgatttca
      841 attgttcttc atttataaat ggattacgtt taaaattatt attttgacta ttgatatgcg
      901 tcatgatatt taaattactg taaatcttat tgatacttct aaactcagta tcatctaata
      961 aataatcgaa atttgagttt aaatgttttt ttataaatga aactctatcc atcttttgat
     1021 gttcatttaa atcgcttaaa cttcttaata acaacaaatc ttcataaacc tcaactgtta
     1081 catcaaattt caaataatca actaagtttc catacttatc aataatttct tctagcgtag
     1141 gtatcaatat atacccccct ccccattttt tgttccgcgt ctaatcataa tgcttcattt
     1201 atgcttatta tagcaatcaa ttgaagatag tttaacctat atgatattga ttgactcctt
     1261 aaaaattatt ggaggaattt taaaatgaat aagaaatcta agcagcaaga aaagctttat
     1321 aattttataa ttgctaaatc ttttcaacaa ccagttggaa gtacgttcac ttatggtgaa
     1381 ttaagaaaga aatataatgt ggtttgtagc acgaatgatc aacgtgaagt aggacgtcgt
     1441 tttgcttact ggattaagta cacgccagga ttaccattta aaattgtagg aacaaaaaat
     1501 ggcagtttat tatatcagaa aatagggata aacccatgca ataatagcac gccttcgaaa
     1561 ggaggtgatt gctaatgagt tacacattgt ttgaaattgg cccatttgct ttagattcct
     1621 tcggttggaa agaaacgata ccacctaaaa aagatggtga cagtgaaaaa gtggttagaa
     1681 tggctagccc tattattgtt aatgcaagat tttctgatcc aattactggt gttgaaaagt
     1741 tgattattac taacaacaat ggtaaaaaag acatttttga gtctgatatc ttaacaacac
     1801 gaaacttacc ttctttaatt aaatatggct acagtattaa cgagaagtat attagatcat
     1861 tgagttatgc cttgcaattg atgcgagata gattaccact ttctgaatta tatgagggtg
     1921 taggtatact agagacacct tttggctact tgatatcgct agataaagtt ctcaaatcta
     1981 ttcaatttaa tcagtcctca ccttcatacc ctattgtaga tagtgcttat gacttaactc
     2041 ctaaaggtac atttgataat tggtttaaca tgtatattga cgaggttaaa ggtcatcttc
     2101 ttttagaatt ggcagtcata tttgggattt ctgccttagt gacaagtttt ctgaagcata
     2161 aacatgaaat cgaatttgca ggaattctct ttagttttac aggtcaatcc tcaaccggta
     2221 agtcaaccgc agctgcttta gctgtatcag tcgctggtaa ccctaccaaa ggtaatgaaa
     2281 ctctttttag aagttggaat gccacacgaa acgcacttga aggttatttg agtaacaatt
     2341 atggcattcc tatagtattt gatgagcttt catctacgac acttagagat accacgggtt
     2401 tgttatattc catcgctgaa ggtcaaggtc gtcaacgttc aaacgttcat ggagaagtta
     2461 aaacgcctaa gaattggggt acttctgtca tttcaacgtc agaatacagt atttttaatg
     2521 attcagctca aaacgatgga ttacgtgtaa gaaccatcga gattaatgaa caattcacta
     2581 caaatgcgac caatgctgac aatattaaaa aagctgtagc actaaactat ggtcatgttt
     2641 taccgttagt agctaaatat cttataaatc gtgaagatga agttattcaa tggttttaca
     2701 aggaagtaga ttggtttgaa gctaaattga aagatgaaac aaacaataca ggtattcgta
     2761 tgtttaagcg ttatgcagtc attacaacat ctgcgaaaat attaggtcgt gttttatcta
     2821 cagatattga tatcgctaat ataagagact actttattga ttatcatact catacagtct
     2881 ctgaacgctc attagctgat aaagctattg atgtcatcat tcaatttgtg gcacaaaaca
     2941 gaggtaagtt ttcagatgaa ggtgctttga aaaatatgtt tgagaactat ggattaatct
     3001 cattaaaaga tgaccatatc gaagtaaaaa taattgcgaa cgtttttaaa cagatgctaa
     3061 acaatcatca atttcaagat gttaataatg ttgtcaacgc cttaagagat aaaggtttca
     3121 tattagctga ccgtggacgc caaacaacta aaagaagtgt caaagataat agcggtaaaa
     3181 aacaatcact tgtattttac catctaaaat tagatgtaga gtttgcatct atcctcggat
     3241 taactaaaga taagtcgtta cttcaaaatt ggacaccgtc aaatgataac aaagctgcga
     3301 aagaactttt taaatccgct aatgaaggga taggcccttc aggagttcac gaagatttct
     3361 aattgtcttt atcatacaac tgtgaagcat caaactgaat aatcgacgta ttgaagatag
     3421 aacaaataac ggagtgaaaa acatggatga acttatcgag atattagccg atttggtaat
     3481 tgaagaaatc aatcaaacaa atgactaata tggacgccca acatctcatt gatgttgggc
     3541 ttatatatca attcatccat aaatatatat tatcactgtg aaaggaggag ccgatatgaa
     3601 acaagtcata ggctatttac gtcaaagtac gatgaaacaa caatctcttg cagcacagaa
     3661 acaagctatc gaagcaatag ccgaaaaaca tcatattcaa catatcaact tttatagcga
     3721 caaacaatca ggtcgcaaag ataatcgtag tgggtatcga caaataacac aattaattca
     3781 acaaggacag tgtgacatat tatgctgtta tcgtcttaat aggttgcatc gtaatctgaa
     3841 aaatgcatta aaactcatca aattatgtca aacgtaccat gttcatatct taagcgtaca
     3901 tgatggttac tttgatatgg atcaagcttt cgaccgactc aagcttaata tcttcatcag
     3961 tttagccgaa cttgaatcgg ataacattgg agaacaagtc agaaatgggc ttcaagaaaa
     4021 agcaaagcaa ggtcgattga ttacaacaca tgcacctttt ggttacgaat atcacaacgg
     4081 aacattcatc atcaatcaaa atgagtcacc aacggtaaag gctgtattca attattacat
     4141 taaaggtcat ggttataaga aaattgcaca gttattagaa gaagataaca cgtatatcaa
     4201 tcgacaaccc tatcaagttc gtaacattat catcaatcct aattattgtg gtcgtgtcaa
     4261 caatcaatat ggccaattcg acaatatgtt tccttctatt gtttccacaa gtatatatga
     4321 gcaagcgcag agacttcgat tgcaaaaaca aaccaaacag acaccttcgg ataatcaact
     4381 caaacaaaaa atcaaatgcc catgttgtaa tgcaacactt acaaatatga ccattagaaa
     4441 aaagaatcat acattacgtt actacgtctg tcctaaaaac atgaatgctt cacgctttgt
     4501 ctgtgatttt aaaggcatca atgcacaaac acttgaagat aaagtattag aagtgtgccg
     4561 agacttttat caaaatcaac gcatctacac aaaaattaaa agtgcgattg acaaacgcat
     4621 caaaagacaa agaaacatag aaaaacatca cacattgact caagaacaac tgatagaaaa
     4681 gttggcacaa ggcatcattg atgcagaaac gttcagagag caaacacaat cattacgtca
     4741 acaaccgcaa cgcactacat ctatcaatgg gcatcaaata caacacatca ttcaaaatat
     4801 tattcaaaaa cgtttcacgt taaacatatt gtacccctat attgaaacca ttcacattac
     4861 gaaagataaa aatcttatag gaatctattt caaaaatgaa ccactcaata ttgtcaatca
     4921 aaccatgcaa tcatcgattg cataaatgaa gaaaggatgt aacataatgc aacaacttaa
     4981 aacaaaacgt gtcggtatct atgtacgtgt atcaacagaa atgcaaagca cagaaggtta
     5041 tagtatcgac ggacaaatca atcaaatcaa agaatactgt gacttccatc attttgaagt
     5101 taaagatata tacgctgacc gtggtatttc aggtaaatct atgaacagac ctgagctcca
     5161 acgtatgttg aaagatgcaa aagaaggcaa tatcgactgt gttatggtat acaaaacaaa
     5221 ccgattagct cgtaatacat ctgatcttct caaaattgtc gaagatttac acaaacaaaa
     5281 tgtcgaattt ttcagtttgt cagagcgtat ggaagtcaat acttcttctg gtaaactcat
     5341 gttacagata cttgcgagtt tctcagaatt cgaacgtaat aacattgtcg agaacgtatt
     5401 tatgggtcaa acgagacgtg cccaagaagg ctattatcaa ggcaatttac cactaggtta
     5461 tgacaaaata ccagatagta aacacgagct aatgattaac caacatgaag ctaatattgt
     5521 aaaatatata ttcgagtgct atgccaaagg acatggctat cgtaaaattg ccaatgcatt
     5581 gaatcacaaa ggatatgtca ctaaaaaggg gaaacctttc agtattagtt caatcacata
     5641 catcttagct aaccctttct atatcggcaa aattcaattt gcgaaataca aagattggag
     5701 tgaaaaacgt cgtaaagggc tgaatgataa accagtgata gctgaaggta agcattcccc
     5761 cattattaat caagatttat gggataaagt acaaatgcgt aagaaacaag tcagtcaaaa
     5821 accccaagtc catggcaaag gaacgaatct gcttacaggc attattcact gtccccaatg
     5881 tggcgcacct atggcagcaa gcaataccac gaatactctt aaagacggga ctaagaaacg
     5941 tattcgttac tattcatgta gtaattttcg gaacaagggt tccaaagtat gttcggcaaa
     6001 cagtgtaaga gctgatgtga ttgaagatta tgtgatgaag caaatacttg aaatagtcaa
     6061 aagtgataaa gtcattcaac gcgtagtaac acacgtcaat caagaaaatc aagttgatgg
     6121 cgctgcactt catcacgata ttgcttataa gcaacaacaa tatgatgaag tacaaatcaa
     6181 aataaataac ttgattaaaa ccatcgagga taatccggac ttaacatcag taatcagacc
     6241 aagtattcaa aaatatgaaa agcaactcaa tgacattacg aatcaaatca accaactcaa
     6301 aaatcaacaa aatgaagata agactttatt tgatgccaaa gaaatcagta aactattaca
     6361 acacatcttt catgatatta agcacataga aaaatctcga ctcaaagcat tgtatctatc
     6421 agtgattgat cgcattgata ttaaaaaaga tggtaatcat aaaaaacaat tctatgtcac
     6481 actcaaactt aataacgaaa tcattaaaca acttttcaat aataaacaac tcgacgaagt
     6541 gcatctcagc acttcgtctt tatttttgcc ccaaacacta tatcttacta tttagctaat
     6601 agaatcattg ttataacaat aattttcact atattctttc gcttatttta tctaactatt
     6661 ttcaagaatt gctctttatc aatttcaata acacttatct aatgaaacag ttattgtcgt
     6721 aattttttca taactataca atgcttatat tgaaattgct aaagaacatc ccacaatgat
     6781 tttttatcgg gaaatgattg ggatttgcag attcgaagaa acagaaggat tgtgcaagca
     6841 caatcccaaa atcccgttac tttttataaa taaataatat atataaggaa gcagagacac
     6901 aaccctatca tcaatactta taaataatga gttgattgtt tgtcaaaata ggcggaattt
     6961 caggatttcc ttggcatacc aacgtttgta gcgggttttt atatggaatt ttctcttatt
     7021 cccaccggga ttcgtaataa atgacaaatc aaatatatat tatctcaatg atgtatatga
     7081 actacatcac tattacacta tctaggaggc aataatatga aaaccatcac tcaagaactc
     7141 aaacaatata taacccacct attccaacta tctaataacg aagcttggga atgtgaagcg
     7201 ctggaggaag cagcagaaaa tattctaccg gaacgtttta ttaatgattc tccactggta
     7261 catctcacac tcgagactta tacctactat aatgatgaac tacatgagct aagtatctat
     7321 ccatttctaa tgtatgccaa taaccaactc atcagtgtcg gatacttaga ccattttgac
     7381 atggactttc tgtaccttac agatactcaa aatatcatta ttgatgaacg tcatttacta
     7441 aaacaaggag gtcaggacca tgaataattg gatcatagtt gctcaattat ccgtcacagt
     7501 tatcaacgaa atcatcgaca tcatgaaaga aaagcagaag ggagggaaat agcatgaaaa
     7561 tcaatcgata catcacaaga ggaattaatg aaagtattcc actagacctt caaatcttac
     7621 tttggcacat ggtagaaaaa aaagataacc agcctcatac agattaccta cacattttta
     7681 aattacaaga agatgagaat atactctcaa tcacacatga acaagaacaa cccacataca
     7741 aattggaata tcactataca aactatgaaa aaaatcaaaa tgcattacct aagaaagtct
     7801 acgtcattcg tgaagatgac gtggacgctt tttattatgt gatgctttta ccagaagaat
     7861 actaaaggaa gtgacgatga tgaatacaat caaaagtacg atacacacag aagccatatt
     7921 tagcgatgat gaacagcacc gctatttact caagaaaaca tgggatgaaa agaaagctgc
     7981 atgcacagtg ataacgatgt atcctcattt agacggtgta ttatcactcg atctcacaac
     8041 tgttcttatc ctcaaccaat tagccaattc agaacgatac ggtgctgtat atcttgtaaa
     8101 tctattctct aatattagaa cacccgaaaa cctcaaacat atcaaaaatc catacgatga
     8161 gcacactgat attcatttga tgaaagcgat tagtgaaagt gacacagtga ttcttgctta
     8221 tggtgcctat gcgaagcgac cagttgttat cgaccgtgtc gaacaagtga tggaaatgtt
     8281 aaaacctcat aaaaagaaag taaaaaagct catcaatcca gtaacaaatg aaattatgca
     8341 tccactcaac cctaaggcac gtcaaaaatg gattttgaaa tcatagcaaa tacatggagg
     8401 tcaatatgga aacaaaacaa atcaacatcg ttttactgta aatgatattg ttgaaacaat
     8461 tcctgacaat gaattcgacg aattcagaca tcaccgtggt gcaacttcgt accatcctaa
     8521 aatgatgtta aaagtgattc tatatgccta cacacaatct gtattctcag gtcgtaaaat
     8581 agaaaaaatg cttaatgata gcatccgaat gatgtggcta tcacaaaatc aaaaaccttc
     8641 ttataaaaca attaatcaat ttagagtaaa tccaaaagta gatgctttat tagaatcttt
     8701 atttattcaa ttttacagtc agtgtgtaaa acaaaatctt atagatgata aagctatttt
     8761 tattgatggt acaaaaattg aagcaaatgc caatcgatat acatttgtat ggaaaaagag
     8821 tattcaaaac catgaatcaa agatgaatga ggattctaaa gccctctacc atgaattggt
     8881 aaccaataaa atcataccgg aaattaaaga agatcatgat aatgaattaa caaaagaaga
     8941 aatagatttg attggtagtc acttagataa agaaatcgaa gatttaaacc aacatatcaa
     9001 caatgaaaaa tgtactaaaa caagaaaaca aatacgtctc aaaagaacta aaatcaaaaa
     9061 atacaaaaag caaatcaatg attattttga gcgaaagtat cgatacgaat ttcaaaaatc
     9121 tattttaaag gatagaaata gttattctaa gacagattat gatgcgacat ttatgagaat
     9181 gaaagaagat cacatgaaaa atggacaact taagccaggg tataatttac aaatagcaac
     9241 aaattcccaa tttgttttat cttataatgt gtatcaaaat ccaacggata ctagaacgat
     9301 gattccattt ttaaattcaa ttcaagagac ctacggtcat ttacctgaat atattgtagc
     9361 tgatgcaggt tatggtagtg aatcaaatta taaggcaatt atagatgact ttaatcgaac
     9421 gccactcata acatatggaa tgtttataaa agataaaact aaaaaatata aaagtgacat
     9481 ctttaatact caaaattgga actatgacga aattaatgac gaattcattt gtccgaataa
     9541 taaacggcta ggttttaaaa gatatgccta tcgtcatgat aagtatggtt ataagcgaga
     9601 cttcaaatta tatgaatgtg atgattgttc agaatgtcct ctgaaaaatc aatgtatgaa
     9661 cttcaattca aaaacaaaca aaaaaataat gaagaattat aactgggaat attttaaatc
     9721 ccaaattaac aaaaagcttt cagaaccaga aacaaaaaat atctacagtc aaagaaaaat
     9781 tgatgtggaa cctgtttttg gatttatgaa ggctattttg ggtttcactc ggatgtctgt
     9841 ccgaggactc aataaagtca aaagagaact tggttttgta ttaatggcac ttaatataag
     9901 aaaagtagta gctcaacgag ctgaaaataa tcaaaaaatt tataaaaaag acaatttcta
     9961 tattatttca atagaaattg tcttttttca cttatccaag aactttatgt cccggactca
    10021 ttatgattta tttttttatt gctgacttgc cattaattct gcttgctcta caacttgttc
    10081 cacagccatt ttttgaagat ctggtggata gccatatttc tttaataggc gtctaacttg
    10141 aactctcatt ttagctttag cactgtctcg tttagaccaa tcaacactca tgttatcctt
    10201 aactgtttta gttaactcat gtgcaatggc tcttaactct ttatcgccca aagcttcttt
    10261 agctgtttca tgtgaagctc gatatactac atacaatata cgcaacaata cgcttgtttc
    10321 gattaggttt aggcattgaa tcataaagtg caatatactt aacacgttct ttaatatttg
    10381 aattaaaacc tagtaaatat tgtgctgcca cattatttat gtgctgagat tttaaaatag
    10441 agcattttaa tatcgattca ccataacgta tatgttcatg gcgattcaaa atttttaaaa
    10501 cgtttctatc acatactttt tcacagtcat tgtccatcat tgttttactt atatatagtg
    10561 caggattaaa ccagaatatc attttaaaaa caacataaag ctggttgaat attaagtcat
    10621 gacttttcac atgtgatagt tcatgtagaa taatatattc aatttctttg tcattcatgg
    10681 tttcgactac gacagttggt agtacaattt gggatttcac taaaccaaat accatcggat
    10741 tatcaatgtt tgaactataa ctaattgtta tatgcttttt gtagaactgc atcttacttt
    10801 gacatacttt aagtcgttca ttaagatatg acgattccaa tgacgaactt ttaataacat
    10861 caatttgtcg gaatgcctta atcatataaa ataagcacaa caaactacca aatacccata
    10921 tcaaaagaat catatacgtt atatttgagg tctcaaactg attaacatta attgctaagt
    10981 ctttcgtaac agatgattgt tgaccatcta acatatgact aaccgaagaa gtcgtgtcag
    11041 atacatttcg attcatcata tcttttgaaa atgtaaaatt cgatattttg taaaatggta
    11101 ttaatggaat taacgtggag acgagcacta ataaccaaat cttatgtgac ataatatttt
    11161 gagtatattt tatatagagc attctcacta aaaaaattac acatatcgtg agcaatgaac
    11221 tgattatact taacattaaa aaagatgata acaccttcta cacctccata tcacaaaaat
    11281 tataacatta ttttgacata aatactacat ttgtaatata ctacaaatgt agtcttatat
    11341 aaggagtata ttgatgaaaa agataaaaat tgttccactt attttaatag ttgtagttgt
    11401 cgggtttggt atatattttt atgcttcaaa agataaagaa attaataata ctattgatgc
    11461 aattgaagat aaaaatttca aacaagttta taaagatagc agttatattt ctaaaagcga
    11521 taatggtgaa gtagaaatga ctgaacgtcc gataaaaata tataatagtt taggcgttaa
    11581 agatataaac attcaggatc gtaaaataaa aaaagtatct aaaaataaaa aacgagtaga
    11641 tgctcaatat aaaattaaaa caaactacgg taacattgat cgcaacgttc aatttaattt
    11701 tgttaaagaa gatggtatgt ggaagttaga ttgggatcat agcgtcatta ttccaggaat
    11761 gcagaaagac caaagcatac atattgaaaa tttaaaatca gaacgtggta aaattttaga
    11821 ccgaaacaat gtggaattgg ccaatacagg aacagcatat gagataggca tcgttccaaa
    11881 gaatgtatct aaaaaagatt ataaagcaat cgctaaagaa ctaagtattt ctgaagacta
    11941 tatcaaacaa caaatggatc aaaattgggt acaagatgat accttcgttc cacttaaaac
    12001 cgttaaaaaa atggatgaat atttaagtga tttcgcaaaa aaatttcatc ttacaactaa
    12061 tgaaacagaa agtcgtaact atcctctagg aaaagcgact tcacatctat taggttatgt
    12121 tggtcccatt aactctgaag aattaaaaca aaaagaatat aaaggctata aagatgatgc
    12181 agttattggt aaaaagggac tcgaaaaact ttacgataaa aagctccaac atgaagatgg
    12241 ctatcgtgtc acaatcgttg acgataatag caatacaatc gcacatacat taatagagaa
    12301 aaagaaaaaa gatggcaaag atattcaact aactattgat gctaaagttc aaaagagtat
    12361 ttataacaac atgaaaaatg attatggctc aggtactgct atccaccctc aaacaggtga
    12421 attattagca cttgtaagca caccttcata tgacgtctat ccatttatgt atggcatgag
    12481 taacgaagaa tataataaat taaccgaaga taaaaaagaa cctctgctca acaagttcca
    12541 gattacaact tcaccaggtt caactcaaaa aatattaaca gcaatgattg ggttaaataa
    12601 caaaacatta gacgataaaa caagttataa aatcgatggt aaaggttggc aaaaagataa
    12661 atcttggggt ggttacaacg ttacaagata tgaagtggta aatggtaata tcgacttaaa
    12721 acaagcaata gaatcatcag ataacatttt ctttgctaga gtagcactcg aattaggcag
    12781 taagaaattt gaaaaaggca tgaaaaaact aggtgttggt gaagatatac caagtgatta
    12841 tccattttat aatgctcaaa tttcaaacaa aaatttagat aatgaaatat tattagctga
    12901 ttcaggttac ggacaaggtg aaatactgat taacccagta cagatccttt caatctatag
    12961 cgcattagaa aataatggca atattaacgc acctcactta ttaaaagaca cgaaaaacaa
    13021 agtttggaag aaaaatatta tttccaaaga aaatatcaat ctattaactg atggtatgca
    13081 acaagtcgta aataaaacac ataaagaaga tatttataga tcttatgcaa acttaattgg
    13141 caaatccggt actgcagaac tcaaaatgaa acaaggagaa actggcagac aaattgggtg
    13201 gtttatatca tatgataaag ataatccaaa catgatgatg gctattaatg ttaaagatgt
    13261 acaagataaa ggaatggcta gctacaatgc caaaatctca ggtaaagtgt atgatgagct
    13321 atatgagaac ggtaataaaa aatacgatat agatgaataa caaaacagtg aagcaatccg
    13381 taacgatggt tgcttcactg ttttattatg aattattaat aagtgctgtt acttctccct
    13441 taaatacaat ttcttcattt tcattgtatg ttgaaagtga cactgtaacg agtccatttt
    13501 ctttttttat ggatttctta tttgtaattt cagcgataac gtacaatgta ttacctgggt
    13561 atacaggttt aataaattta acgttattca tttgtgttcc tgctacaact tcttctccgt
    13621 atttaccttc ttctacccat aatttaaatg atattgaaag tgtatgcatg ccagatgcaa
    13681 tgataccttt aaatctactt tgttctgctt tttctttatc tatatgcata tattgaggat
    13741 caaaagttgt tgcaaattgg ataatttctt cttctgtaat atgaaggctt tttgttttga
    13801 atgtttctcc tactataaaa tcatcgtatt tcatatatgt ctctctttct tattcaaatt
    13861 aattttttag tatgtaacat gttaaaggta agtctaccgt cactgaaacg taagactcac
    13921 ctctaacttt ctattgagac aaatgcacca ttttatctgc attgtctgta aagataccat
    13981 caactcccca attagcaagt tggtttgcac gtgctggttt gtttacagtc catacgttca
    14041 attcataacc cgcttctttt accattttta cttttgcttt agtaagtttg gcatcttcag
    14101 tgtttactat tttagcatta cagtaatcta aaagtgttct ccagtcttca cgaaacgaag
    14161 ttgtatggaa tataactgct ctgttatatt gtggcatgat ttcttctgca agtttaacaa
    14221 gcacaacatt aaagcttgaa atgagcactt cttgattctg atttaagttt gttaattgtt
    14281 cttccacttg cttaaccata cttttagaaa gtgctagtcc attcggtcca gtaatacctt
    14341 ttaattctac atttaaattc atattatatt catttgctat ttttactaca tcatcgaaag
    14401 ttggcaaatg ttcatctttg aatttttcac caaaccaaga tcctgcagaa gcatctttaa
    14461 tttcatcata attcaattca gttatttccc cggacatatt tgtagtccgt tctaaataat
    14521 catcatgaat gataatcagt tgttcatctt ttgtaattgc aacatctaac tccaaccagt
    14581 ttataccttc tacttctgaa gcagctttaa atgatgcaat tgtattttcc ggagctttac
    14641 taggtaatcc tctatgtcca tatacagtta gcatattacc tctccttgca tttttatttt
    14701 tttaattaac gtaactgtat tatcacatta atcgcacttt tatttccatt aaaaagagat
    14761 gaatatcata aataaagaag tcgatagatt cgtattgatt atggagttaa tctacgtctc
    14821 atctcatttt taaaaaatca tttatgtccc aagctccatt ttgtaatcaa gtctagtttt
    14881 tctgtacccc ttatctgcaa ttttacttag gattgctttt aacttacccc ttatcagcaa
    14941 ttttactgag aactgctttt aacgcacctc ttatctgcaa ttttgcctag aactgctttt
    15001 aacgtacctc ttatctgcaa ttttactgag aactgctttt aacttacccc ttatcagcaa
    15061 ttttgcatgg aattgctttt aacgtacctc ttatctgcaa ttttacttag aactgctttt
    15121 aacaaacctc ttatctgcaa ttttacttag aactgctttt aacgtacctc ttatctgtaa
    15181 ttttactgag aactgctttt aacaaacctc ttatctgcaa ttttacttag aattgctttt
    15241 actattcctc ttattagtat aatctcagta agaatgcgta taaaaatgaa aattacaacc
    15301 gattttgtaa gtgctgacgc ctgagggaat agtatgtgcg agagactaat ggctcgagcc
    15361 atacccctag gcaagcatgc acgtacaaaa tcgtaagata aaaaaataag catatcactg
    15421 taaactttaa aaaatcagtt tagtgatatg cttatttatt tcgagttagg atttatgtcc
    15481 caagctcatc aagcacaatc ggccactagt ttatttctct atcttatatg ttctgatatg
    15541 gtcttctata ctgtataagt atacttttga atatggatct tgtgtcaatt cacgttcgaa
    15601 atcaaattct tgattatcaa atctgttaaa gaatgtttcg tattcttcga ctgataattg
    15661 ctctctagat tctagcatat ttaagtgttt ctctttatct aatgctttgt catatccttt
    15721 aacgattgaa ccactaaaga tttctcctac tgctcctgaa ccataactaa atagacatac
    15781 tttctcttct ggttggaatg tgtggttctg taataacgaa attaaactta agtataatga
    15841 tcctgtataa atgttaccaa catctctatt ccataatacg gttctgttgc aaagttgaat
    15901 ttatagtata attttaacaa aaaggagtct tctgtatgaa ctatttcaga tataaacaat
    15961 ttaacaagga tgttatcact gtagccgttg gctactatct aagatataca ttgagttatc
    16021 gtgatatatc tgaaatatta agggaacgtg gtgtaaacgt tcatcattca acggtctacc
    16081 gttgggttca agaatatgcc ccaatttt
//
LOCUS       DALAGF010000064        15926 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.064, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000064 DALAGF010000000
VERSION     DALAGF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15926
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.064"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(134..1831)
                     /locus_tag="O2692_001793"
     CDS             complement(134..1831)
                     /locus_tag="O2692_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCZ4299419.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(1831..2049)
                     /locus_tag="O2692_001794"
     CDS             complement(1831..2049)
                     /locus_tag="O2692_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCZ4299420.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            complement(2078..2200)
                     /locus_tag="O2692_001795"
     CDS             complement(2078..2200)
                     /locus_tag="O2692_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299421.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
     gene            complement(2530..3081)
                     /locus_tag="O2692_001796"
     CDS             complement(2530..3081)
                     /locus_tag="O2692_001796"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCZ4299422.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
     gene            3446..4072
                     /locus_tag="O2692_001797"
     CDS             3446..4072
                     /locus_tag="O2692_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HCZ4299423.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            4243..5355
                     /gene="pdhA"
                     /locus_tag="O2692_001798"
     CDS             4243..5355
                     /gene="pdhA"
                     /locus_tag="O2692_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCZ4299424.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            5359..6336
                     /locus_tag="O2692_001799"
     CDS             5359..6336
                     /locus_tag="O2692_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCZ4299425.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            6427..7719
                     /locus_tag="O2692_001800"
     CDS             6427..7719
                     /locus_tag="O2692_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCZ4299426.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            7723..9129
                     /gene="lpdA"
                     /locus_tag="O2692_001801"
     CDS             7723..9129
                     /gene="lpdA"
                     /locus_tag="O2692_001801"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCZ4299427.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNGIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            9297..9572
                     /locus_tag="O2692_001802"
     CDS             9297..9572
                     /locus_tag="O2692_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCZ4299428.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            9716..10255
                     /locus_tag="O2692_001803"
     CDS             9716..10255
                     /locus_tag="O2692_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4299429.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            10268..11362
                     /locus_tag="O2692_001804"
     CDS             10268..11362
                     /locus_tag="O2692_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4299430.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            11355..12152
                     /locus_tag="O2692_001805"
     CDS             11355..12152
                     /locus_tag="O2692_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4299431.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAASITIITLTISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            12158..12967
                     /locus_tag="O2692_001806"
     CDS             12158..12967
                     /locus_tag="O2692_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4299432.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            12967..14040
                     /locus_tag="O2692_001807"
     CDS             12967..14040
                     /locus_tag="O2692_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HCZ4299433.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDRGNKYSLPYFFGTVGILYNKEKYPNESFDSWKS
                     LYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVG
                     DEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKE
                     GAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVY
                     KDLGPEVLSEYNENFLNFKMSLK"
     gene            14114..15133
                     /locus_tag="O2692_001808"
     CDS             14114..15133
                     /locus_tag="O2692_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCZ4299434.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            15416..15835
                     /locus_tag="O2692_001809"
     CDS             15416..15835
                     /locus_tag="O2692_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCZ4299435.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH"
BASE COUNT         5665 a         2287 c         2882 g         5092 t
ORIGIN      
        1 ctactgccaa tataactttg tagagcattg aacattgatt tatgtctcaa gctcatcata
       61 tataaagaaa aagctactaa caatgggtgg tgagtttaat taaaacttca cttcaaagtt
      121 agtagctttt tgattattta ttgtttgatt ctttttgttc gtttaccttc ataatgactg
      181 gtaaaatcat tggttttcta gctgtttttt caaataaata aggttgtaat gtttcaatga
      241 tagaagattt aatctgatgc cattgaatat ctttattttg atttaactta ctaataacat
      301 cagttttgat tttgcgttgt gcatcataaa ttaattgacc tgattccctc atatatacaa
      361 atcctcgaga aataatgtct ggaccagaaa gtaatttatt tgtattaaaa tcaatactaa
      421 caacaacgat aactaaacct tcttcagata atagcttacg gtctcttatt acaacattac
      481 cgatatcacc gataccacta ccatcaacaa gtacattacc agatggaatg cgaccagctt
      541 tacgtgctga atcgtgtgtt aaagctaaga catctccaat atcaaagatg aagacattat
      601 cttcttcaac gccgcattca acaccagtct caccatgtgc ttttaacata cggtattcac
      661 catgaatagg taagaaatat ttcggcttga ttaatcgaag cattaattgt tgatcacctt
      721 gagaaccatg ccctgaagta tggatgttag aaatcttgct atggataaca tctgcaccag
      781 ctttatacaa ggaattaata gttctgttaa tactttttgt attacctggg ataggtgatg
      841 aactaaatac aacggtatct tcaggtataa ttttaatttg cttatgagta ccattagcaa
      901 ttctagataa tgctgccatt ggttcacctt gtgaaccagt acatagtatc aataactcat
      961 gcttcggtac ggtattaatt ttattaggtt caataaatgt ttcaggtggt gctttaatat
     1021 aaccaagttc catacctatt ttaatattgt tttccatcga acgaccgaac gtaacaattt
     1081 tacggttatt tttgatagca gcttcaactg cttgttgaac tcggtaaata ttagaagcga
     1141 aggtagcaaa tataatacga cctttacaat tacggaagat cttatctacg ttttgaccaa
     1201 cttcacgttc gcttaaagta aaatcaggca caagtgaatt tgttgagtct gaaagtaaac
     1261 atagaacgcc ttcttcgcct aattgagcca ttttagcaat gtttgctggt ttgcctacag
     1321 gtgtaaaatc aaatttaaag tcaccggtat gaactacttt tccttcaggt gtatctacga
     1381 tgacgccata agtttcagga atactatgtg tagttaagta gaaagaaatc gtaaagtgct
     1441 tagatttaat cacactgtcc tcattgattt catttagttt agcagtacgt aataaatgat
     1501 gttcttcaag tttattacgg attaaaccta atgctaaagg accaccataa ataggtatat
     1561 taagttgttt taataggaag ggcacaccgc ctatatggtc ttcgtgacca tgtgttataa
     1621 ataggccaac aattttatct tggttttgaa ctagatatgt gtagtcaggt ataacataat
     1681 caatccctaa taagttatca tcagggaatt tgatacctgc atcgataatg acaatttcgt
     1741 ctttatactc aactgcataa gtatttttac cgatttcacc tagacctcca agtgcatata
     1801 cacctacttc atttggatgt aattgtttca ttatttagca atctccacat taaagtgttc
     1861 tgagttttct ttttcgtaat ctaaatgtgc gccctctaat ttagtgataa attcgatatt
     1921 aaaattacga tctttcaagt aacgacgtac ttgttcttct gtttgagctt caacataaag
     1981 tgattgtgta ttttcacgca caattacctc gtctctgtta tgttgataaa aaactttaaa
     2041 tactgccatg ttaaaaattc ctcctaagaa tgtttgttta atttattgtt aaccctagta
     2101 aaatcgtatt ggagtatata tcgataaatt cattccaatc atctctataa tgtaacttaa
     2161 taacgatttg gttaataact aggttcatca tgtcgttcat tttaaaaaat tagtgaaata
     2221 acactaaaat ttcagttaaa tcttatttta catgatgaat gataataaat aaagcagttt
     2281 atctcactag aaatctaagt atttcgaata aatgtgcagt tctatttatt aaaataaatg
     2341 tagagattat aaattaatgt taagaaagaa ttaaaggatt ttatatgaaa cggtaaaatt
     2401 taaatgaggg gataaaaagt ttggtagaaa ggtgaatata tttaactgtt gtttattcga
     2461 aaaagttgta aaaggaaaag cttaggacat catatttatc ctaagctttg ataattagaa
     2521 aaatgtgttt taaacttcta ctgcatctgt atgtggttgt aatgggtgat ctttgtcaat
     2581 gtgatcatag aacattacac catttaaatg gtcaatttca tgttggaaaa caattgctgg
     2641 atatcctttt agtcgtaatt gtatatcatt accttcgatg tctttggctt taattgtaat
     2701 tctattatga cggtgaacta gaccagcaac attatcatcg acactaaggc aaccttcacc
     2761 agttggtaaa taagcttctt gaacgctatg acttacaatt tttgggttca caagcatata
     2821 gtcataagat ttgccactgc catcatctgg tattaaaaca gcaatcatac gtttagaaat
     2881 attaatttga ggtgcagcca aaccaacgcc tgaacgtaaa ccatatcgtt tcgcgatttc
     2941 ctcatcttga ctatttacta aaaactctct catggcgatt aatgtttctt tttcttcttt
     3001 agttaatggt aattctaact cagctgcttt ttgacgcaaa gttggatgac catctctaat
     3061 gatgtctttc attgttaaca tatattgcac cttccttatt ttaatttgtt ttagttgaat
     3121 gacagtaaaa aggttgttaa gatactcata catttttatg tgtaaatatc tacaaagtta
     3181 accaactact gccaatgttt attttagata gtatatgtaa attttcagat atgctaattg
     3241 cttaaaaaat gattaaagtg ttggtttcaa gcaatgatac tttagaaatt tatttatcat
     3301 cttgacttta aaaattatat tataaatgac gtaactgtca acagatatac ttagtaatga
     3361 agatgtgtaa tgtaattgtt taaaattgat ttccaagcag attttattta tcatttaatt
     3421 taaatagcaa gtggaggtac aagtaatgaa atttggaaaa acaatcgcag tagtattagc
     3481 atctagtgtc ttgcttgcag gatgtactac ggataaaaaa gaaattaagg catatttaaa
     3541 gcaagtggat aaaattaaag atgatgaaga accaattaaa actgttggta agaaaattgc
     3601 tgaattagat gagaaaaaga aaaaattaac tgaagatgtc aatagtaaag atacagcagt
     3661 tcgcggtaaa gcagtaaagg atttaattaa aaatgccgat gatcgtctaa aggaatttga
     3721 aaaagaagaa gacgcaatta agaagtctga acaagacttt aagaaagcaa aaagtcacgt
     3781 tgataacatt gataatgatg ttaaacgtaa agaagtaaaa caattagatg atgtattaaa
     3841 agaaaaatat aagttacaca gtgattacgc gaaagcatat aaaaaggctg taaactcaga
     3901 gaaaacatta tttaaatatt taaatcaaaa tgacgcgaca caacaaggtg ttaacgaaaa
     3961 atcaaaagca atagaacaga actataaaaa gttaaaagaa gtatcagata agtatacaaa
     4021 agtactaaat aaggttggta aagaaaagca agacgttgat caatttaaat aattaatata
     4081 atacagatgg taggaaacaa ctaatacagt tcctattatc tgtatctttt tttattaaaa
     4141 cagaactttt tcaaatggtt taacagtccc atttatttgt ggtacaatta gtaaggataa
     4201 aatgaatttc tatacaatta tgggaaaggt atggtgaatt gaatggctcc taagttacaa
     4261 gcccaattcg atgcagtaaa agttttaaat gatactcaat cgaaatttga aatggttcaa
     4321 attttggatg agaatggtaa cgtcgtaaat gaagacttag tacctgatct tacggatgaa
     4381 caattagtgg aattaatgga aagaatggta tggactcgta tccttgatca acgttctatc
     4441 tcattaaaca gacaaggacg tttaggtttc tatgcaccaa ctgctggtca agaagcatca
     4501 caattagcgt cacaatacgc tttagaaaaa gaagattaca ttttaccggg atacagagat
     4561 gttcctcaaa ttatttggca tggtttacca ttaactgaag ctttcttatt ctcaagaggt
     4621 cacttcaaag gaaatcaatt ccctgaaggc gttaatgcat taagcccaca aattattatc
     4681 ggtgcacaat acattcaagc tgctggtgtt gcatttgcac ttaaaaaacg tggtaaaaat
     4741 gcagttgcaa tcacttacac tggtgacggt ggttcttcac aaggtgattt ctacgaaggt
     4801 attaactttg cagcagctta taaagcacct gcaattttcg ttattcaaaa caataactat
     4861 gcaatttcaa caccaagaag caagcaaact gctgctgaaa cattagctca aaaagcaatt
     4921 gctgtaggta ttcctggtat ccaagttgat ggtatggatg cgttagctgt atatcaagca
     4981 actaaagaag cacgtgaccg cgcagttgca ggtgaaggtc caacattaat tgaaactatg
     5041 acatatcgtt atggtcctca tacaatggct ggtgacgatc caactcgtta cagaacttca
     5101 gacgaagatg ctgaatggga gaaaaaagac ccattagtac gtttccgtaa attccttgaa
     5161 aacaaaggtt tatggaatga agacaaagaa aatgaagtta ttgaacgtgc aaaagctgat
     5221 attaaagcag caattaaaga ggctgataac actgaaaaac aaactgttac ttctctaatg
     5281 gaaattatgt atgaagatat gcctcaaaac ttagcagaac aatatgaaat ttacaaagag
     5341 aaggagtcga agtaagccat ggcacaaatg acaatggttc aagcgattaa tgatgcgctt
     5401 aaaactgaac ttaaaaatga ccaagatgtt ttaatttttg gtgaagacgt tggtgttaac
     5461 ggcggtgttt tccgtgttac tgaaggacta caaaaagaat ttggtgaaga tagagtattc
     5521 gatacacctt tagctgaatc aggtattggt ggtttagcga tgggtcttgc agttgaagga
     5581 ttccgtccgg ttatggaagt acaattctta ggtttcgtat tcgaagtatt tgatgcgatt
     5641 gctggacaaa ttgcacgtac tcgtttccgt tcaggcggta ctaaaactgc acctgtaaca
     5701 attcgtagcc catttggtgg tggcgtacac acaccagaat tacacgcaga taacttagaa
     5761 ggtattttag ctcaatctcc aggtctaaag gttgttattc cttcaggccc atacgatgcg
     5821 aaaggtttat taatttcttc tattagaagt aatgacccag tcgtatactt agagcatatg
     5881 aaattgtatc gttcattccg tgaagaagta cctgaagaag aatatacaat tgacattggt
     5941 aaggctaatg tgaaaaaaga aggtaatgac atttcaatca tcacatacgg tgcgatggtt
     6001 caagaatcaa tgaaagctgc agaagaactt gaaaaagatg gttattctgt tgaagtaatt
     6061 gacttacgta ctgttcaacc aatcgatgtt gacacaattg tagcttcagt tgaaaaaact
     6121 ggtcgtgcag ttgtagttca agaagcacaa cgtcaagctg gtgttggtgc agcagttgta
     6181 gctgaattaa gtgaacgtgc aatcctttca ttagaagcac ctattggaag agttgcagca
     6241 gcagatacaa tttatccatt cactcaagct gaaaatgttt ggttaccaaa caaaaatgac
     6301 atcatcgaaa aagcaaaaga aactttagaa ttttaataca ttttaaaagt taacgaagtt
     6361 agcgtatttt agtctcattg attaaaatga aatgtttaat ttacgaaatc ttaggagggc
     6421 aaaaacgtgg catttgaatt tagattaccc gatatcgggg aaggtatcca cgaaggtgaa
     6481 attgtaaaat ggtttgttaa agctggagat actattgaag aagacgatgt tttagctgag
     6541 gtacaaaacg ataaatcagt agtagaaatc ccatcaccag tatctggtac tgtagaagaa
     6601 gttatggtag aagaaggtac agtagctgta gttggtgacg ttattgttaa aatcgatgca
     6661 cctgatgcag aagatatgca atttaaaggt catgatgatg attcatcatc taaagaagaa
     6721 cctgcgaaag aggaagcgcc agcagagcaa gcacctgtag ctactcaaac tgaagaagta
     6781 gatgaaaaca gaactgttaa agcaatgcct tcagtacgta aatacgcacg tgaaaaaggt
     6841 gttaacatta aagcagtttc tggatctggt aaaaatggtc gtattacaaa agaagatgta
     6901 gatgcatact taaatggtgg tgcaccaaca gcttcaaatg aatcagctgc ttcagctaca
     6961 agtgaagaag ttgctgaaac tcctgcagca cctgcagcag taacattaga aggcgacttc
     7021 ccagaaacaa ctgaaaaaat ccctgctatg cgtagagcaa ttgcgaaagc aatggttaac
     7081 tctaagcata ctgcacctca tgtaacatta atggatgaaa ttgatgttca agcattatgg
     7141 gatcaccgta agaaatttaa agaaatcgca gctgaacaag gtactaagtt aacattctta
     7201 ccttatgttg ttaaagcact tgtttctgca ttgaaaaaat acccagcact taacacttca
     7261 ttcaatgaag aagctggtga aatcgttcat aaacattact ggaatatcgg tattgcagca
     7321 gacactgata gaggattatt agtacctgtt gttaaacatg ctgatcgtaa gtctattttc
     7381 caaatttcag atgaaattaa tgaattagct gttaaagcac gtgatggtaa attaacagcc
     7441 gatgaaatga aaggtgctac atgcacaatc agtaatatcg gttcagctgg tggacaatgg
     7501 ttcactccag ttatcaatca cccagaagta gcaatcttag gaattggccg tattgctcaa
     7561 aaacctatcg ttaaagatgg agaaattgtt gcagcaccag tattagcatt atcattaagc
     7621 tttgaccaca gacaaattga tggtgcaact ggccaaaatg caatgaatca cattaaacgt
     7681 ttattaaata atccagaatt attattaatg gaggggtaaa acatggtagt tggagatttc
     7741 ccaattgaaa cagatactat agtaatcgga gcaggtcctg gtggatacgt tgcagcaatt
     7801 cgtgcagctc aattaggaca aaaagtaaca atcgttgaga aaggtaatct tggtggtgtt
     7861 tgcttaaacg taggatgtat tccttcaaaa gcattactac atgcttctca ccgttttgtt
     7921 gaagcacaac attctgaaaa cttaggtgtt attgctgaaa gtgtttcttt aaacttccaa
     7981 aaagttcaag aattcaaatc atcagttgtt aataaattaa ctggtggtgt tgaaggctta
     8041 cttaaaggta acaaagttaa catcgttaaa ggtgaagcat atttcgtaga taacaatagc
     8101 ttacgtgtta tggacgaaaa gagcgcacaa acatacaact ttaaaaatgg aatcattgca
     8161 acaggttcaa gaccaattga aattcctaat ttcaaattcg gtaaacgtgt tatcgactca
     8221 acaggtgctt taaacttaca agaagtacca ggtaaattag ttgtagttgg tggaggatac
     8281 attggatcag aattaggtac agcatttgct aactttggtt cagaagtaac catccttgaa
     8341 ggtgctaaag atatcttagg tggcttcgaa aaacaaatga cacaacctgt taaaaaaggt
     8401 atgaaagaaa aaggtgttga aatcgttact gaagctatgg ctaaatcagc tgaagaaaca
     8461 gataacggag ttaaagttac ttatgaagct aaaggcgaag agaaaacaat cgaagctgat
     8521 tatgtattag taactgtagg tcgtcgtcca aacacagacg aattaggcct agaagaatta
     8581 ggtgttaaat tcgctgaccg tggattatta gaagttgata aacaaagccg tacgtctatc
     8641 agcaatatct atgcaattgg tgatatcgtt ccaggtttac cacttgctca caaagctagc
     8701 tatgaagcta aagttgctgc tgaagcaatt gatggtcaag ctgctgaagt tgattacatt
     8761 ggtatgccag cagtatgctt tactgaacca gaattagcta cagttggtta ttcagaagcg
     8821 caagctaaag aagaaggttt agcaattaaa gcttctaaat tcccatatgc agcaaatggt
     8881 cgtgcattat cattagatga tactaacgga tttgttaaac ttattacact taaagaagat
     8941 gatactttaa tcggtgctca agtagttggt actggtgcat cagatattat ctctgaatta
     9001 ggtttagcaa ttgaagctgg tatgaatgct gaagatatcg cattaacaat ccatgcacat
     9061 ccaacattag gtgagatgac tatggaagca gcagaaaaag ctatcggata cccaatccat
     9121 acaatgtaat aactgattat ctataaagat tcagtcatta aaagctgtag catatgctac
     9181 ggcttttttg ttttaggtaa agtaatgtaa ggaaattgat ttgagatatc gttaacatgt
     9241 gacatgcatg ttatactagc gatgctaaca aaagaattga aatggagggt tcaacaatgg
     9301 aatatgagta tccaattgat ttagactgga gtaatgaaga gatgatttca gtgataaatt
     9361 tctttaatca tgtagagaag tattatgaat ccggcgtgac ggcaggcgac tttatgggtg
     9421 catataaaag atttaaagaa attgtgcctg ctaaagcaga ggaaaaacaa atttttaata
     9481 ctttcgaaaa aagtagtggc tataatagtt acaaagcagt tcaagatgta aaaactcact
     9541 ctgaagaaca aagagtaaca gctaaaaaat aattcgttcg aaattaacac aatttaatag
     9601 gaatttttct ttaaaactat tgctaataaa gctatatttt gataccttta tcaagtgtta
     9661 aacaaaatgt ttgataaaag taaacttaat atagcttttt taggtggaaa aataaatgaa
     9721 cataggtaat aaaattaaaa atcttagaag aattaaaaat ttaacgcaag aagaacttgc
     9781 tgaacgtaca gacttatcga aaggctacat ttcacaaata gaaagtgaac atgcctcacc
     9841 aagtatggaa actttcttaa atattataga ggtgttagga acgacgccaa gtgaattttt
     9901 taaagacagt gaaaatgaaa aagtattata caagaaggaa gaacaagtta tttatgatga
     9961 gtatgatgaa ggttatatat taaattggtt agtttcaaag tcaaatgaat atgatatgga
    10021 gccattaata ttaactttaa agcctggagc atcatataaa aattttaatc catcagagtc
    10081 tgatacgttt atttattgta tgtcaggtca gataacactt aatttaggca aagagatata
    10141 tcaagcacaa gaagaagacg ttttgtattt taaagcacga gataatcatc gtttgtcaaa
    10201 cgaatcaaac aatgaaacac gaatacttat tgtagcgaca gcttcatatt tatagggggg
    10261 atcttatttg gaaccgttat tatcattaaa atcagttagt aaaagctatg atgatcttaa
    10321 tatcttagat gacatagata ttgatattga atcaggatac ttttatacat tattaggtcc
    10381 ttcaggttgt ggtaaaacaa caattttaaa attaattgca gggtttgaat atcctgacag
    10441 tggtgaagtg atttatcaaa acaaaccaat tggtaattta ccaccaaata aacgtaaagt
    10501 gaatacagtc tttcaagatt atgcattatt tccacactta aacgtctatg ataatatcgc
    10561 ttttggtttg aaattaaaaa aattatcaaa aaccgaaatt gatcaaaaag taactgaggc
    10621 attaaaatta gtaaaacttt caggttatga aaaaagaaat attaatgaaa tgagtggcgg
    10681 acaaaagcaa cgtgttgcaa ttgcacgtgc tatcgtaaat gaaccagaaa tattattgtt
    10741 agatgaatct ttatccgcat tagatttgaa attgcgtact gaaatgcaat atgaattacg
    10801 agaattgcaa tctagattag gtattacatt tatatttgta acacatgatc aagaagaagc
    10861 gttagcatta agtgactttc tttttgtatt aaaagatggg aaaattcaac aatttggcac
    10921 accaacagat atatatgacg aaccagtgaa tcgatttgta gctgatttta ttggagaatc
    10981 taatattgtt gaagggcgca tggttagaga ttatgtcgtg aatatttatg ggcaagattt
    11041 cgaatgtgtc gatatgggta ttcctgaaaa taaaaaagta gaagtcgtta ttcgaccaga
    11101 agatatatca ttaatcaaag ctgaagaagg attatttaaa gcaactgttg attctatgtt
    11161 atttagaggg gtccactatg aaatatgttg tatagacaat aaaggttatg aatgggtaat
    11221 acaaacgact aaaaaagctg aagtaggcag tgaagttggt ctttattttg atcctgaagc
    11281 cattcatatt atggttcctg gagaaacaga agaagaattt gataaacgta ttgaaagcta
    11341 tgaggaagta gacaatgcgt aatactaata aatttctctt aatcccgtat ttactatgga
    11401 tggttatatt tattattgta ccagttgtat tactcattta tttttcattt ttagatatca
    11461 atggacattt tagtttcacg aattatcaac aaatttttac tacaaaatat ttgaaaatgt
    11521 ttgcatattc aattttatat gccgcttcaa taacaattat taccttgact atcagttatc
    11581 cagctgccta ttatattact cgttcgaaat ttcaaaatat cttattaatg ataatgatta
    11641 ttccaacatg gataaattta ttgttaaaga catatgcttt tataggttta ttaagtcatg
    11701 atggcgtgat taatcaattt ttccacttat ttaatttacc atcattcaat ttgttattta
    11761 caactggtgc gtttttagta gtggcaagtt acatttatat accatttatg attttaccta
    11821 tatttaatag catgaaagca attcctaata atttattgca ggcctcaagt gatttaggtg
    11881 ccagtccttt ctatactttt agaaaagtaa tcatgccgtt aacaaaagaa ggtgttatga
    11941 ctgggattca agtaaccttt attccatcac tttcattatt tatgattaca agattaattg
    12001 caggtaataa agtcataaat ataggtacgg caatagagga acaattttta acaattcaaa
    12061 attatggtat gggatcaact atagctatat tcttaattgt atttatggca ttcattttaa
    12121 tcattacaaa atcatctaat gggagagggt gaatcatatg aaatggtatg gaaagctgta
    12181 tatcgggata cttttagcga ttttatacat cccaatattc tttttaatgt tctattcatt
    12241 caattcggct ggtaatatga ttcactttga acattttaca ttagagcatt atcaatcatt
    12301 atttcaaaat gatcatttaa tgtcggtcat ttttaatacg atagctgtag cacttttagc
    12361 agcctcaatt tctacagtta ttggtacatt tggtgccatt gctatttatt atttaagaaa
    12421 taaaaagttt aaagtaactt tactaacatt gaataatgtc ttgatggtat catccgacgt
    12481 tgtcataggt gcatcattct taattatgtt tacaacgatt ggccatttta ctggtcttgg
    12541 tttaggattt tggacagttc taatatctca tattgcattt tgcataccta tagttgtgat
    12601 tatcgtctta ccacaactgt atgaaatgaa taataatatg ttaaatgctg caagagattt
    12661 aggagcgact gaaccacaat tattaagcaa cattattatt cctaatattt taccttctat
    12721 tataggagga ttctttatgg ctttaactta ttcactagac gatttcacag taagtttctt
    12781 cgttactggt aatggcttta gtgtgttatc agttgaagtt tatgctatgg cgagaaaagg
    12841 aattagtatg gaaattaatg cgatttcaac attattgttt gctgttattg tattaggaat
    12901 actaggatat tatttgattc aatacgtgat aaataagaaa aaactaatca agcgaggtgt
    12961 aaaataatga aacgattttt acaactcatt ataggtgcat tagttgtggg tatgctttgt
    13021 cttactttaa gtcattggtt taaatctaaa gaacaagtgc atacaaatca aaaaatttac
    13081 gtatacaatt ggggcgaata tattgatcca gagttaatta agaaatttga aaaagaaact
    13141 ggcattcaag tcgtttatga aactttcgat tcaaatgaag cgatggaagc caaaattcgc
    13201 aatggcggta cacattatga tgttgctttt cctagtgaat atacagttca aaaattgaaa
    13261 agagatcatt tattgttacc aatagatcat aataaggtac ctaatattaa aaatttagat
    13321 tcagattata tgaatatgtc atttgataga ggcaataaat attctttacc ttatttcttt
    13381 ggaactgtag gtattttata taataaagaa aagtatccaa atgaatcatt tgatagttgg
    13441 aagtcattgt ataatcctaa atttaaaaac caaattttac tagttgacgg tgctagagag
    13501 attataggca tgagtttgaa taaacttggg tataatctta atgaccgtaa ttcgcaccat
    13561 ttaaaagaag cagagcgaga tttaaccaaa ctagcaccac aagtaagagg tgtcgtaggt
    13621 gatgaaatta ccatgatgct tcaacaaaat gaaggtaaca tagcggttgt ttggagtggt
    13681 gttgcagcac ctctagtgca agaaggggat aaatataatt atgttattcc taaagaagga
    13741 tcgaatttat ggttcgacaa tatggtaatt ccaaaaacgg cacaaaataa agagggtgca
    13801 tataaattta tgaatttttt attagatgct aaaaataaca agcaaaatac agaattcgta
    13861 ggctatgcaa cgccaaacaa ggctgctcga caattgttgc ctaaagagat taaagacgac
    13921 catcgttttt atccgactaa gaaagagcaa gaacgccttg aagtttataa agatttagga
    13981 cctgaagttt taagtgaata caatgaaaac tttttgaatt tcaaaatgtc attaaaataa
    14041 aatcataaat agtagttatt tactgtatat ttagttataa taacgggata agtactataa
    14101 ggagttgtaa cgaatgactg gagaacaatt tactcaaatt aaacgtccag taagtagatt
    14161 aactgaaaaa gttctaggtt ggttatgttg ggtgatgtta ttagtgctta ctgtcattac
    14221 gatgtttata gcacttgttt cattcagtaa taacacatcg attgctaatc ttgaaaatac
    14281 attaaacaat aatgcattta tccagcaatt attagctgga aatggctata atacaacaca
    14341 atttgtaata tggttacaaa atggtatatg ggctattatc gtttacttta ttgtttgttt
    14401 gttgatttca tttttagctc tcatatctat gaatataaga atcttgtcag gtttcttatt
    14461 cttaatatca gcaattgtaa cgattccttt agttttactt atcgttactt taatcattcc
    14521 gatattattc tttattattg cgatgatgct atttataaga aaagataaag ttgaaatggt
    14581 tgcgccacaa tattatgaag agtataacgg accgatttat gattatcgtg aacctgtgta
    14641 tgagcgcccc caaccgaaag atgattatta tgatgtgcct aaatatgaaa aagaattgga
    14701 taaatcaaat actgtatatg atcaagaaca ggaaagagat aaatatgatc aatttcctaa
    14761 acgtgcagtt gaaagtgaat ataatcatga tgagcgcact gaggaagaac catcagtatt
    14821 atccagacag gctaaataca aacaaaaaag tactgaagaa ctaggtattg aagatgatgg
    14881 ttattatgca gaacctgaag ttgatccaaa agaattgaaa gcacaacaaa agcgagaaaa
    14941 agctgaaatt aaagctaaga aaaaagaaaa acgtaaagct tataaccaac gaatgaaaga
    15001 acgtaggaaa aatcaaccta gcgcagttag tcaacgtcga atgaattttg aagagcgacg
    15061 tcaaatttac aacaatgata tttctgaaga acgcaattca agtgaagtta aggacaaaaa
    15121 agagcaagaa taaatattga tgatacccag attagtaagc agaggtcttt tgcttattaa
    15181 tctgggtttt tatatgaggt taattatcga taacgtttaa ttaaagtgtt taggtgtcat
    15241 aattttaaat gacgatttct cattactata cacctaaatt atcatcaatc tgaattcaga
    15301 tgtttattat aaaaattaga tgaaaaatat gttaatatac aagtaattta atgtgcgtat
    15361 atctataggc tcgtagtata cttattaaaa tattaaacaa agaaaggatt ttaagatgaa
    15421 tagaaaacct gaattaatca tggcttggat tgcaaatagt attagtatta tttatttatt
    15481 agttatgggg ctatcctatt tttctttaaa aagtggtaat gcatcacaac gtgaagaatt
    15541 agcgaagcaa ttatctcaga acggtggcaa ggtttcttta gatatgcttc agacaacaat
    15601 gggtgcatta gcaattattt tattaatttc aacactttat ggtatatttg cgacaatttg
    15661 tattaaagga cgtagaaaat tatcgattat actttttgtt atcgcgataa ttgtaagttt
    15721 gatggctctt aatttaattg caattgtctt atgggttatc gtgatgatta tgttgatttc
    15781 taaaaaagaa tcaaaagaaa caacacataa ggacgatgag tatatttatc attaatatgt
    15841 tcatagcaaa agaaaaacca ttaaaatgtt aagttgttaa ttattagata caaccaacat
    15901 tttaatggtt ttatttttaa ctttgt
//
LOCUS       DALAGF010000065        15924 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.065, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000065 DALAGF010000000
VERSION     DALAGF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15924
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.065"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..1156
                     /gene="scpA"
                     /locus_tag="O2692_001810"
     CDS             <1..1156
                     /gene="scpA"
                     /locus_tag="O2692_001810"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HCZ4299436.1"
                     /translation="FPKLIALSLIFSLSVTPIANAESNSNIKAKDKKHVQVNVEDKSV
                     PTDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVLNTEG
                     NIDYIVTISPKITKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVVTQNH
                     KAKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQYINK
                     LENFKIRETQGNNGWCAGYTMSELLNATYNTNKYHAEAVMRFLHPNLQGQRFQFTGLT
                     PREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGHAMAV
                     VGNAKLDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY"
     gene            1187..1513
                     /locus_tag="O2692_001811"
     CDS             1187..1513
                     /locus_tag="O2692_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HCZ4299437.1"
                     /translation="MEQIELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKHKS"
     gene            complement(1805..1978)
                     /locus_tag="O2692_001812"
     CDS             complement(1805..1978)
                     /locus_tag="O2692_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HCZ4299438.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIVFVGKKIQ"
     gene            complement(1959..2561)
                     /locus_tag="O2692_001813"
     CDS             complement(1959..2561)
                     /locus_tag="O2692_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCZ4299439.1"
                     /translation="MSFVTENPWLMVLTIFIINICYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            complement(2832..3653)
                     /gene="nadE"
                     /locus_tag="O2692_001814"
     CDS             complement(2832..3653)
                     /gene="nadE"
                     /locus_tag="O2692_001814"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCZ4299440.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            complement(3646..5115)
                     /locus_tag="O2692_001815"
     CDS             complement(3646..5115)
                     /locus_tag="O2692_001815"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCZ4299441.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            5299..6375
                     /locus_tag="O2692_001816"
     CDS             5299..6375
                     /locus_tag="O2692_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HCZ4299442.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            6395..7189
                     /locus_tag="O2692_001817"
     CDS             6395..7189
                     /locus_tag="O2692_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCZ4299443.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNFFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            7261..7368
                     /locus_tag="O2692_001818"
     CDS             7261..7368
                     /locus_tag="O2692_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299444.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(7379..8941)
                     /locus_tag="O2692_001819"
     CDS             complement(7379..8941)
                     /locus_tag="O2692_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCZ4299445.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGYEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(9146..10243)
                     /locus_tag="O2692_001820"
     CDS             complement(9146..10243)
                     /locus_tag="O2692_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HCZ4299446.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGRRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            10615..11175
                     /locus_tag="O2692_001821"
     CDS             10615..11175
                     /locus_tag="O2692_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCZ4299447.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            11228..12157
                     /locus_tag="O2692_001822"
     CDS             11228..12157
                     /locus_tag="O2692_001822"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCZ4299448.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(12352..12525)
                     /locus_tag="O2692_001823"
     CDS             complement(12352..12525)
                     /locus_tag="O2692_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299449.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            12577..13956
                     /locus_tag="O2692_001824"
     CDS             12577..13956
                     /locus_tag="O2692_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCZ4299450.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(14076..15104)
                     /locus_tag="O2692_001825"
     CDS             complement(14076..15104)
                     /locus_tag="O2692_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCZ4299451.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            15374..15775
                     /locus_tag="O2692_001826"
     CDS             15374..15775
                     /locus_tag="O2692_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HCZ4299452.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
     gene            complement(15793..15924)
                     /locus_tag="O2692_001827"
                     /pseudo
     CDS             complement(15793..15924)
                     /locus_tag="O2692_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5378 a         2793 c         2337 g         5416 t
ORIGIN      
        1 ctttccaaaa ttaatagctt taagtttaat attttcttta agtgttaccc ctattgcaaa
       61 cgctgagagc aattcaaata tcaaagcaaa agataagaag catgtgcaag ttaatgtcga
      121 ggacaagagt gttcctactg acgtacgtaa tttagcgcaa aaagactatc tttcttatgt
      181 tacttcactt gataaaatct ataataaaga gaaagcctcg tatacacttg gtgaaccttt
      241 taaaatttat aaattcaaca aaaagagtga tggtaattat tattttccag ttttgaacac
      301 tgaaggtaat atcgattata tagttacaat ttcaccaaaa atcactaagt attctagttc
      361 atcttctaaa tacacaatca atgtttctcc atttttatct aaagtcttaa atcaatataa
      421 agatcaacaa attactattt tgacaaactc aaaaggttac tatgtggtta ctcaaaatca
      481 taaagctaaa ctagtactaa aaacaccacg tctagaagat aaaaagttaa aaaagactga
      541 gagtatacct actggcaata atgtaacgca actaaagcaa aaagcttctg tcactatgcc
      601 aactagtcaa tttaaaagta ataattatac ttacaacgag caatatataa ataaacttga
      661 gaatttcaag ataagagaaa ctcaaggtaa caatggttgg tgtgcaggct atacgatgtc
      721 tgaattacta aatgcgactt ataatactaa caaatatcat gcagaagctg tgatgagatt
      781 tttacaccca aacttacaag gacaacgatt ccaatttact ggtttaacgc caagagagat
      841 gatttatttt ggacaaactc agggaagaag tccacaactt cttaatagaa tgactacata
      901 taatgaagtt gacaatctta ctaaaaataa taaaggaatt gctgttttag gtagtagagt
      961 agaatcaaga aatggtatgc acgcagggca tgccatggca gttgttggga acgctaaatt
     1021 agataatgga caagaggtta taattatatg gaatccatgg gataatggtt ttatgactca
     1081 agatgctaaa aacaatgtta ttccagtatc caatggcgac cattatcgat ggtattcatc
     1141 tatttatggt tattaaaatt tgtattttag aaaggtaaat aatattatgg aacaaattga
     1201 attatttagt attgataaat tcaaatgtaa ttcagaggct aagtattatc ttaatattat
     1261 tgagggagaa tggcatcctc aagatttaaa tgatagtcct ttaaaattta ttctcagtac
     1321 ctcagacgat tctgattaca tttgcaaata tataaataca gaacacaaac aactcacatt
     1381 atataataaa aataatagct caattgttat tgaaatattt ataccaaatg ataataaaat
     1441 actactaaca attatgaaca cagaagcttt gggaacttct cctagaatga ctttcattaa
     1501 gcataagtca taagactatc tagatatgat taagtaaact attaatctta ttaaaggtct
     1561 ctaggaagaa aaaatttcat gcgatgagat tgttttagaa aaagcgacga tatatataat
     1621 ctaacaataa tttttaaaat attaagaacc cccagcatat gctttatata gcttgctggg
     1681 ggtttctcat acaatatgga tcgagcatct tgcatgtata aacaaatagg ctattcaaag
     1741 taaaattact caaactttga atagcctttc ctcttcaaat agtctttatt taaattggat
     1801 tatgttattg gattttctta cctacaaaaa cgatttcttg atgtgatact tcaaccgtgc
     1861 catctttttg ctcttgaaac acgggttttt caatacgttt taccggcata tacccttgca
     1921 ttttcattcg tgacaaacaa tcagcaattg tttcattctc atttactttg aatttcatgt
     1981 tctactacac tttccagttc ttctggttca taatctttaa gctttctacg acgaatgcct
     2041 ttagtccaga atccaccatg tatgtttcga ggttcatacg caatgataaa tgctttcgga
     2101 tctaaatttt ttatcgtatc catcaattta cgttcatatt ttcttggtgt taaaatttgc
     2161 atcaccatac gactaccatc tctaccaaac gcagcatagt gcgtaacgcc atatcctaaa
     2221 tttcgaagtt cattcggtaa atctaactca tattctgctg aagttacatt tacaactgta
     2281 taacctaatg ccagtttttc ttctattttc ataccaacaa tgatacctat tgaaaaacca
     2341 aatgcgtagg caataatatt ttgaatatgg tctaaattag acataaccaa acctaaacca
     2401 acgatataaa ctaatacttc taaaaaacta actgatgcag caatataacg ataacctttc
     2461 aacgttaaaa ttgttcgcat cgttaaaaac gttacataac aaatgttaat gataaatata
     2521 gttagtacca ttaaccatgg attttctgtt acgaatgaca taaggcgccc cactctcttt
     2581 caattacact ttaaatacca aactgcttca acctaacctt aatgaatgca tattaatata
     2641 atatagtacg aaaactctca taaataaata gcgaatttgt cataaataaa aatttattct
     2701 caaatgaaca tacttacttt agttcataca ctttcaattt atacactgaa gtagtctagc
     2761 tgaatgtggt ttaatgttat taattctaac tcatatttaa tttaagtcac acgttagaag
     2821 aaaaaattaa attaggattt tggccacgtg tatcttgtat atgcaagttc acgtttgtgt
     2881 gcatttcgta tataatgatt ttcaattact ttttgttctt ctggcgtaac tggcttacct
     2941 tctaaataat tatcaatcgc ctcataagtt acacctaatg catcttcatc tggaagctgt
     3001 ggtttatcat cttctaaatc agcagttggc gttttttcat ataattcctt tggcgcacca
     3061 agatacgcta ataattgacg accttgtcgt ttattcaaac caaatatagg tgcgatatct
     3121 gcagcaccat caccgtactt cgtataaaac ccagttatat tttcagctga atgatctgtt
     3181 cctactacaa taccttgtcg gtttgaagca attgaaaatt gtactttcat acgttcacgc
     3241 gctttttcat ttcctttttg gaaatctgta agaacaatac cggcttcttt taatgattgc
     3301 acactttgat caactgcagg cttaatattg actgttacta tttcatctgg ttcaatgaat
     3361 cgcaaagctt gctcaacttc atcagcatct ttttgaactc cataaggtaa tttaactgca
     3421 ataaacgtac aatcaatgcc ttcttcacgt aattcgttaa cagacatttg tactagtttt
     3481 ccaactaatg tagaatcctg tcctcctgaa atacctaaca ctaaagattt tataaatgaa
     3541 tgtgattgta cataattttt tataaattgc tttaattcca taatttcttc agcactatcg
     3601 atacgctttt tcactttcat ttcttgtaca ataacgtctt gtaatttact cattatcttc
     3661 ttccatctcc ttaacgtgtt ccgcaacttc aaaaatacgt ttatgtttat tatcccaaca
     3721 tgccttgctt aaatcgactg gatattcttg tggattcagg aaacgcttat tttcatccca
     3781 aatagattgt aatcctagtg ctaaatattc acgtgattca tcttctgttg gcatttgata
     3841 tactaattta ccattttcat aaatattatg atgcaaatca atggcttcga aagattttat
     3901 aaatttcatt ttataagtat gcactggatg gaataatttt aaaggttgtt catcgtatgg
     3961 attttcattt tccaaagtaa tataatcgcc ttctgcctta cctgttttct tgtttataat
     4021 gcgatataca tttttcttac ctggcgtcgt aaccttttca gcgttatttg ataatttaat
     4081 acgatcacta tatgaaccat cttcattttc aatagctaca agtttatata ctgcacctaa
     4141 tgctggttga tcgtatcctg taatcagctt tgtaccaacg ccccaagaat ctacttttgc
     4201 accttgtgct ttcaaactcg taatcgtttc ttcatccaaa tcattagacg cgataatttt
     4261 agtttcagta aatcctgctt catcaagcat acgtcttgcc tctttagata aataagcgat
     4321 atctccagaa tctaatcgaa tacctacaaa gttaattttg tcacctaatt cttttgcaac
     4381 ttttattgca tttggcacgc cagattttaa agtatggaat gtatctacta ggaacacaca
     4441 atttttatgt ctttcagcat attttttgaa ggcaacatat tcgtctccat aagtttggac
     4501 aaatgcatgt gcatgtgtac cagacacagg tataccaaat aatttccccg ccctaacatt
     4561 acttgtagaa tcaaagcccc cgatgtaagc agctctagcg ccccacaatg ctgcatcaat
     4621 ttcttgcgca cgacgtgtac caaactccat taatttatca tttgatgcaa tttgacgaat
     4681 tctgctagcc tttgttgtaa ttaatgtatg gaaatttaca atgtttaata aaattgtttc
     4741 tattaattgc gcttgaatca atggtgcttc tacgcgtaac aatggttcgt taccaaagca
     4801 taattcgcct tcttgcatcg aacggatgct gcctgtgaat tttaaatctt ttaaatatga
     4861 taagaaatca tccttgtagc caatagactt taaatattcc aaatcagatt ctgaaaatcc
     4921 aaaatgttct ataaaatcaa tgacgcgttt taaaccatta aaaacagcat agccactatt
     4981 aaatggcatt tttctaaaat acaaatcaaa tacagccatt ttttcatgaa tattatcatt
     5041 ccaataactt tcagccatat ttatttgata taagtcatta tgtaacatta aactgtcgtc
     5101 ttctaattgg tacacttgta tctctccaat cgacctaaat attttcttac attttatcat
     5161 aattcatttt tttatataca taagagcccc ttaatttcca tacttttaat taaaatcaac
     5221 caacaattta atgacatata cataattttt aagagtattt taataatgta gactataata
     5281 taaagcgagg tgttgttaat gttatttaaa gaggctcaag ctttcataga aaacatgtat
     5341 aaagagtgtc attatgaaac gcaaattatc aataaacgtt tacatgacat tgaactagaa
     5401 ataaaagaaa ctgggacata tacacataca gaagaagaac ttatttatgg tgctaaaatg
     5461 gcttggcgta attcaaatcg ttgcattggt cgtttatttt gggattcgtt aaatgtcatt
     5521 gatgcaagag atgttactga cgaagcatcg ttcttatcat caattactta tcatattaca
     5581 caggctacaa atgaaggtaa attaaagccg tatattacta tatatgctcc aaaggatgga
     5641 cctaaaattt tcaacaatca attaattcgc tatgctggct atgacaattg tggtgatcct
     5701 gctgaaaaag aagttacacg cttagcaaat cacttaggtt ggaaaggaaa aggtactaat
     5761 tttgacgtgt taccactgat ttaccaatta cctaatgagt cagttaaatt ttacgaatat
     5821 cctacttcat taattaaaga agtacctatt gaacataatc attatccaaa attaagaaaa
     5881 ttgaacttaa aatggtatgc agtccctatc atttccaata tggacttaaa aatcggtggc
     5941 attgtatatc caactgcacc ctttaacggt tggtatatgg taactgaaat tggcgtacgt
     6001 aactttattg atgattaccg ttacaattta ctagaaaaag ttgcagatgc gtttgaattt
     6061 gatacactta aaaataattc atttaataaa gatcgagcac ttgttgaatt gaactatgct
     6121 gtgtatcatt cctttaaaaa agaaggcgta tcaattgtcg atcatttgac cgctgcaaag
     6181 caattcgaac tattcgaacg taacgaagca caacaaggtc gtcaagttac cggaaaatgg
     6241 tcttggctag caccgccatt atctccaaca ttgacgtcaa attatcatca cggatatgac
     6301 aatacagtaa aagatccaaa ctttttctat aaaaagaaag aatcaaatgc taaccagtgc
     6361 cctttccatc attaagttag tagaggtgta gcatatgcaa ttatattatt taggccctaa
     6421 aggtacattt tcatatttgg cttgtcgtca gtatttttct gaaaatgaag caacttttca
     6481 accaaagtca aatttttttg aagtcatcaa ggcagttgca gatgatgaca catcgattgg
     6541 tgttgtccct attgaaaatt ctatagaagg aacaatcaac attgtggcag atgcacttgc
     6601 acagcaagat gtctttgcac acggtgaaat acgtttagat attaactttg cattatatgg
     6661 taatggaaca gattccattt ctgatattaa aaaagtgtat tctattgcac cagcaatcag
     6721 tcaaacaaca aattacatac atcaacatca atttgactat gattatgtcg acagtacaat
     6781 tcaaagtttg actaaaattg aaaatggcgt tgcagcaatt gcacctttag gtagtggcga
     6841 agcttatggt tttacaccta ttgatacaca tattgaagac tatccacata acgtgacacg
     6901 ttttttagtt ataaaaaatc agcagcaatt tgatcaaaat gcgacatcat tgatgttttt
     6961 aattacacca atgcatgata aacctgggtt gttagcaagt gtgcttaata catttgcttt
     7021 gtttaatatt aatttatcct ggattgagtc tcgtccttta aaaacacaat taggtatgta
     7081 tcgatttttc gttcaggctg attctgcaat tacaaccgat ataaagaaag tcattgctat
     7141 tttagaaacc ttagatttca aagtagaaat gattggtgct tttaattaaa ctttattttc
     7201 taggttctac aatttaaata aaataaagag tcaaccaagg attaaaatag gaatccatcc
     7261 ttggttgact ttcttttgtt tgtattgttt ttacacgatg ctgcggtata cgtttcgtta
     7321 ccactcgtcc aagcaattgc taatgtaacc ttttattttt cgttctaatc tgcaattact
     7381 atttcaatgg cagtggttga tttatatcaa tacctaaaac tataggcatt acataataca
     7441 cgactaaaat aataattatt gcactgatta agtttaccca gaatcctact gatgccattt
     7501 gtttgataga tattttacca gaaccaaaga taattgcatt cggtggtgtc cctactggta
     7561 acatgtatgc acagttagcc gccatagctg caggtgccat aagtagtaat ggatgcactc
     7621 caacagcaac agacaacgtt gctaaaatcg gtaaaatcat cgttgcagtt gcagtattag
     7681 atgtcacttc agttaaaaat aagacaaaga ttgttatgac aattacaata agaatcggac
     7741 taacaccatt taatgatttc aactgttcgc ctaaccattt tgctaaacca ctttcagaaa
     7801 tacctttcgc taatgctaaa ccgccaccaa ataaaattaa tacaccccaa gggagctctt
     7861 ttgcaacttc ccagtcaatg atacggcgat gtttttcagt atttttagct ggaataataa
     7921 ataataatat tgatataaaa atagcaatcg taccatctgc aacagatgac gtaacttccc
     7981 attttttcag aagaaactct cttgtaatcc ataataagct agcaagtaca aagatagttt
     8041 gtactacctt ttcttcatac ttcattttgc caagctcgtc taacttttgt ttaattaacg
     8101 tctgaccacc aggtaaatat ttcaaatcat gtctaaacgc aacatatctt aaatagagcc
     8161 aagtaatacc taacaaaaca atgaccgttg gaatccctac aatcatccat ttagcaaaac
     8221 taatttcata tccaaaatgt tgcatgtatt gtccttttaa aataattaat ggcggggttc
     8281 cgattaatgt acccaagcca ccaatcgtac ctgcatagcc aattgctaaa actagagatt
     8341 tttcaaactt ttgaatactt gtttgattcg tattggcttc ttgtaaatca tgtgcttcct
     8401 taataattgc taaaccaatc ggaatcataa tcattacagc tgcagtgttc gatacaaaca
     8461 tagataagaa tcctgttgcc accatgaatc caagtaatat ttttgaagtg cttgcaccaa
     8521 tcaaattaat aattgtcaac gctactctcg tatgtaaatt ccatctttcc attgcaattg
     8581 ccaaaataaa tccacctaaa aacaaaaaga taatatcatt gccatattcg gatgatactt
     8641 gttctggtgt aagtatatga cctaatggta ataacacaat tggtaataag ctcgttgctg
     8701 caataggaat tgcttcagta atccaccaag tcgcaatcca taaagtaatc gctaaaacat
     8761 agacgccttt ccaaggtaag tcttgtggat gaaagaataa taatgttaat aggaaaagta
     8821 atggacctaa tattagtcca ataagttgtc ccgcactaaa cggtttcttt ttctttgatt
     8881 ttgaaaagaa tgttaaatac cttttcgata tcattgattg atgttgattg aaataagcca
     8941 taaatttccc cctcatgatg attatgacaa gctacttcaa ctcactatat tagagtatag
     9001 aaagttgtct atcttgtatt atcattaccg tcaaaatacc ccttttatca gataattggg
     9061 tttattatag catatttata ccgttttatg aatgcgcttt caaaaattat ctactattct
     9121 taatcctaat gtcatgcgaa actttttact tttttatttc atctacattg attgtagtaa
     9181 cttgaatacc cgcttcaaca ggacttaaaa acactgtatc aatatcttct aaatgaattg
     9241 attgagtcga attattgaat gtattcccaa cgattaatac atctttatgt ttaacattat
     9301 tataattacg tacatgtatc gcatctttag acattgatcc tttaaattca tttccaatta
     9361 tattcatatt tatgcctgct tgggaaccta agtcttttcc tgtcatcaca tctgctgcat
     9421 ttttaccatc tctaactcct aaatatctaa tgccaccctc acagttaata aacttattat
     9481 taataatgaa agcatcttta tacttcaagg gagttaatgc ataaccttgt atatcttcaa
     9541 atatattatt tctaatatga atattctcat agtatcgatt atgtctactt gcatgtgagc
     9601 caatagcacg attccaactt cccatttcgg gcaattctga aggtccaaaa taacaatctt
     9661 cgataatgac atttttcgtt atcgtaccat ctgtcgttcc gaattttgga aaagcaccag
     9721 gtacttgaat gtctaattgt attgcttcag aataaaagcg ttcgccacta tagtctatga
     9781 atccttcaaa tgaacagctt ttaatataga gtccgttaat cccacaagca tcaattgcat
     9841 gaccactcac tacattctta atggtcacac ctattaattg aatatcttca gcatgcccaa
     9901 tgcacatagc tgtattgtta taaggatacg atacaccatt catatcaaac ttgccgcctt
     9961 taatatgaat atgactgtgt ccattataac cacgataaaa accaaagcga cgaccatttt
    10021 ttaataaagg accagaatga catcggcgta aaatagtttc attatctaac aaaagtgttg
    10081 tattgccata tatcgttaat ggtttgcaaa tatcatacgt ccctttcggt atataaactg
    10141 tcgttggttt acatcttcca cgatttaacg cacgttgtat tgctcgagta tctttagttt
    10201 tgtttatgcc tgttaaccca taatctttca tattgatata cataaatcgt catcctttgt
    10261 ctattttcta attactcata tattaaccaa ttattcaaat tataatcgtg ttaggttgat
    10321 ttaacagata gtcataaatc tcgtgatgcc atctattagc atttttatat tcaaaacatc
    10381 aacacttgat attagttcta agtttgtcac atttagtatt ctattacagc tattttaaaa
    10441 ctatcgcaaa aacttcttat attatttgtt gattgcgtga tttgctcttc caaaaaagtt
    10501 gattaatcta tatacttaat ctatatttat atattaacca ttagggttaa aaattactct
    10561 agcatttatg aatagatggg agtttatttt attattatat aggagagatg ttgaatgaca
    10621 catcgcgcac tattagttgt tgactattca tatgacttta tcgcagacga cggcttacta
    10681 acatgcggta aacctggaca aaatattgaa gattttattg tttctcgtat caatgacttt
    10741 aattattatc aagaccatat attcttttta atggatttac attatttaca tgacattcat
    10801 catcctgaaa gtaaattatt cccaccacac aatatcgtag atacaagtgg tagagaatta
    10861 tacggtaaag taggtaaatt atacgaaaca attaaagcgc aacctaatgt acatttcatt
    10921 gataaaacgc gctatgattc gttctttggt accccgcttg atagtttatt gagagaaaga
    10981 agtattaatc aagtcgaaat cgttggtgta tgtaccgata tttgcgtgtt acatacagca
    11041 atttctgcat acaacttagg ttataaaatt tcagtacctg ctgagggagt ggcttcattt
    11101 aatcaaaaag ggcatgaatg ggcacttgca catttcaaaa actcattagg tgcagaggta
    11161 gaacaacacg tttaaatcgt gctaaaataa ttataaagaa tacaatttac aagggagata
    11221 tttgacaatg gctaaaacat atattttcgg acataagaat ccagacactg atgcaatttc
    11281 atctgcgatt attatggcag aatttgaaca acttcgaggt aattcaggag ccaaagcata
    11341 ccgtttaggt gatgtgagtg cagaaactca attcgcgtta gatacattta atgtacctgc
    11401 tccggaatta ttaacagatg atttagatgg tcaagatgtt atcttagttg atcataacga
    11461 attccaacaa agttctgata cgattgcctc tgctacaatt aagcatgtaa ttgatcatca
    11521 cagaattgca aatttcgaaa ctgctggtcc tttatgttat cgtgctgaac cagttggttg
    11581 tacagctaca attttataca aaatgtttag agaacgtggc tttgaaatta aacctgaaat
    11641 tgccggttta atgttatcag caattatctc agatagctta cttttcaaat caccaacatg
    11701 tacacaacaa gatgttaaag cagctgaaga attaaaagat attgctaaag ttgatattca
    11761 aaagtacggc ttagatatgt taaaagcagg tgcttcaaca actgataaat cagttgaatt
    11821 cttattaaac atggatgcta aatcatttac tatgggtgac tatgtgactc gtattgcaca
    11881 agttaatgct gttgaccttg acgaagtgtt aaatcgtaaa gaagatttag aaaaagaaat
    11941 gttagctgta agtgcacaag aaaaatatga cttatttgta cttgttgtta ctgacatcat
    12001 taatagtgat tctaaaattt tagttgtagg tgctgaaaaa gataaagttg gcgaagcatt
    12061 caatgttcaa ttagaagatg acatggcctt cttatctggt gtcgtttctc gaaaaaaaca
    12121 aatcgtacct caaatcactg aagcattaac aaaataatac tatattactg tctaattata
    12181 gacatgttgt atttaactaa cagttcatta aagtagaatt tatttcactt tcaatgaact
    12241 gttttttatt tacgtttgac taatttacaa ccctttttca atagtagttt ttattccttt
    12301 agctacccta acccacagat tagtgatttc tatacaattc cccttttgtc tttacatttt
    12361 cttaaaatat ttgcgatgtt gagtataaat ttttgttttc ttcctacctt tttcgttatg
    12421 attaaagtta taaatattat tatgtacacg attcatcgct ctattttcaa ctttcaacat
    12481 atataattcg aaagaccatt taaaattaac ggccacaaca ttcaaatcaa ttaatcgctt
    12541 tttccaaaat aatcatataa ggaggttctt ttcattatga atatcattga gcaaaaattt
    12601 tatgacagta aagctttttt caatacacaa caaactaaag atattagttt tagaaaagag
    12661 caattaaaga agttaagcaa agctattaaa tcatacgaga gcgatatttt agaagcacta
    12721 tatacagatt taggaaaaaa taaagtcgaa gcttatgcta ctgaaattgg cataactttg
    12781 aaaagtatca aaattgcccg taaggaactt aaaaactgga ctaaaacaaa aaatgtagac
    12841 acacctttat atttatttcc aacaaaaagc tatatcaaaa aagaacctta tggaacagtt
    12901 ttgatcattg caccatttaa ctatcctttt caactagtgt tcgaaccttt aatcggtgct
    12961 attgcagcag gtaatacggc aattattaaa ccatctgagt tgacgcctaa tgttgcacga
    13021 gtgattaaac ggttaatcaa tgaaacattt gatgcaaatt acattgaagt tattgaggga
    13081 ggaattgaag aaacgcaaac gttaattcac ttaccttttg actatgtctt ctttacagga
    13141 agtgaaaatg taggcaaaat cgtttatcaa gctgccagcg aaaatttagt tcctgtgaca
    13201 ttagaaatgg gcggtaaatc tccagtcatc gttgatgaaa cagcgaatat taaagttgct
    13261 agtgagcgca tttgttttgg gaaattcact aatgccggcc aaacatgtgt tgcaccagat
    13321 tacattttag tacacgaatc tgtaaaagat gatttaatca cagccctatc aaaaacgttg
    13381 cgtgaatttt atggtcaaaa tatacaacaa agtccagatt atggccgcat tgtaaacctt
    13441 aaacattatc atcgtctgac ttcattactt aacagtgcac aaatgaatat tgtatttggt
    13501 ggtcatagtg atgaggatga acgttatata gaaccaacat tgttagatca cgttacaagt
    13561 gattcagcaa ttatgcaaga agaaattttt ggtcctatct taccgatttt aacgtatcag
    13621 tcattggatg aagcaatagc ctttattcac caaagaccaa aacctttgag tttatattta
    13681 tttagcgaag atgaaaatgc tacacaacgt gtaataaacg agctatcatt tggcggcggc
    13741 gctattaatg atacattgat gcacctagcg aatcctaaat taccatttgg tggtgttggc
    13801 gcctcaggta tgggacgcta tcatggtaaa tattcattcg acacttttac acatgaaaaa
    13861 agctacattt tcaaatctac acgattagaa tcaggtgtcc atttaccacc atataaaggt
    13921 aaatttaaat acatcaaagc tttctttaaa aattaatttt tgagacaaaa ataaaccatg
    13981 cttccataat ttgatgttct tgaaaacaat aaatttcaag gtatacacaa attttttgga
    14041 aacatggttt tctttttatc atgattaatt aaagattaaa atatgacaca tacaccttca
    14101 gatgctacac gagtgttatc acatagcgta attttacctg tttctttatt tctttcgaaa
    14161 acagttacaa ctgaatctcc ttgctcatga gcacaaacta aaaggtcatc tgatgaggca
    14221 atattaaaat ctcttgggaa ttgaccacca ctttcagtaa ttgttactag ttctaagtgt
    14281 tgaccattat caagaacttt aaaaattgca atgctatcat gccctctatt agatacatat
    14341 aagaattgtt gatcatgaga taaacgcact gctgcaagtt tagtatctcc atcaaagttt
    14401 tcaggaattg ttaaatgacg ctcgagctct tcaaatttac cgtcattata ttctgcaaca
    14461 ctcacagtat ttgataattc gtgtacgaca taagcaaatt ttccattatc atgaaattca
    14521 atatgtcttg tcccatcact atctttaaat aaagattctt tataaaattc aaacccgttg
    14581 tcatcaaatt tataagtaac gatacgatca gcacctaaat ctgttactgc aacgtactta
    14641 ccatctggag tttgattaat ataatgtgcg tgtggatgat cttgtctttc atgtgtacct
    14701 gttggaaaat catgtgcaag ttcttgaatt agacgtataa tttcacctgt gtgcgtattt
    14761 aattcataca tacgtatgat gccagcacca tataccgctt caaataaata tcgtttatct
    14821 tctgaaatcg atacataaca acctgtacca gcttttgaag acaaacattt atttaataaa
    14881 tgcaattcac cattgtcatc aattttcaaa cttgcaacac cgcattgttc tccttctttg
    14941 ttgattccat ataaaacttc attattacgc accaaatatg tagacgcttc taattcaaat
    15001 cctgtttcta ataaatcaat acgtgactga ttttcgttta attcaaaacg ataaatccct
    15061 ttaccattct ttttagtgta agaaccaata tatccatttg tcattacttt tacccctcca
    15121 tgttagtctt attttcattt tattaaattt tagctagaat taacaatcat ttatcatttc
    15181 cttaaagcta aaactattta actactttaa aagaactatt tgctgtgata tgtcattatt
    15241 cgattttaat taatccctct tttttactta cacgagcatt aattattaac gcgtttatgc
    15301 ttccacaaaa tttttgttgc aaaatagaac acatgttcgt ataatgtaat tatcagattg
    15361 gggtgaatta aggatgacaa gttatgaaga agaaactgat tatcgtaacg ttccaagaga
    15421 attactagat aacaatatac ctatgggaag aggcatgatt aaatgggctc cttttgcaac
    15481 attacccgaa caattcgaaa cgattcaaca atacattatc gaccaaaaca aaattacacg
    15541 tcctgtatta tcagatgatc aattggctga gctaaacatt cggctacacg aggcgctaca
    15601 atatgcccaa cctgtagaag ttaaatttta taataatggc tttgtagatt cagtacgctt
    15661 aaccatttat cgtattgatg ctattaatta tgaaattgat ggttatgttt acaatcagca
    15721 acaacgtcaa aaaatttcta tctttgatat cttagatatt gtcttgttac cttaatgctt
    15781 tgaacataat taaacagatt gagtatatgc atataagatg atttttaaca tcatttttgg
    15841 atgataggat gttgcgccac gatgatgtct gaattcatcg aattcgctat caggtattgt
    15901 ttcaacaatt tcattaacat atcg
//
LOCUS       DALAGF010000066        15828 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.066, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000066 DALAGF010000000
VERSION     DALAGF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15828
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.066"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(62..1189)
                     /locus_tag="O2692_001828"
     CDS             complement(62..1189)
                     /locus_tag="O2692_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCZ4299453.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSNIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(1551..1928)
                     /locus_tag="O2692_001829"
     CDS             complement(1551..1928)
                     /locus_tag="O2692_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCZ4299454.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(2344..2937)
                     /locus_tag="O2692_001830"
     CDS             complement(2344..2937)
                     /locus_tag="O2692_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCZ4299455.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            3001..3384
                     /locus_tag="O2692_001831"
     CDS             3001..3384
                     /locus_tag="O2692_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HCZ4299456.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHDRKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            3515..5920
                     /gene="mnhA1"
                     /locus_tag="O2692_001832"
     CDS             3515..5920
                     /gene="mnhA1"
                     /locus_tag="O2692_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HCZ4299457.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            5913..6341
                     /gene="mnhB1"
                     /locus_tag="O2692_001833"
     CDS             5913..6341
                     /gene="mnhB1"
                     /locus_tag="O2692_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HCZ4299458.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            6341..6682
                     /gene="mnhC1"
                     /locus_tag="O2692_001834"
     CDS             6341..6682
                     /gene="mnhC1"
                     /locus_tag="O2692_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HCZ4299459.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            6675..8171
                     /gene="mnhD1"
                     /locus_tag="O2692_001835"
     CDS             6675..8171
                     /gene="mnhD1"
                     /locus_tag="O2692_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HCZ4299460.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVYFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            8173..8652
                     /gene="mnhE1"
                     /locus_tag="O2692_001836"
     CDS             8173..8652
                     /gene="mnhE1"
                     /locus_tag="O2692_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HCZ4299461.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            8652..8945
                     /gene="mnhF1"
                     /locus_tag="O2692_001837"
     CDS             8652..8945
                     /gene="mnhF1"
                     /locus_tag="O2692_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HCZ4299462.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            8923..9279
                     /gene="mnhG1"
                     /locus_tag="O2692_001838"
     CDS             8923..9279
                     /gene="mnhG1"
                     /locus_tag="O2692_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HCZ4299463.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            9528..10682
                     /locus_tag="O2692_001839"
     CDS             9528..10682
                     /locus_tag="O2692_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCZ4299464.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKELDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(10738..11112)
                     /locus_tag="O2692_001840"
     CDS             complement(10738..11112)
                     /locus_tag="O2692_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HCZ4299465.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(11131..12447)
                     /locus_tag="O2692_001841"
     CDS             complement(11131..12447)
                     /locus_tag="O2692_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HCZ4299466.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(12858..14333)
                     /locus_tag="O2692_001842"
     CDS             complement(12858..14333)
                     /locus_tag="O2692_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HCZ4299467.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVFYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSEVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(14464..15672)
                     /locus_tag="O2692_001843"
     CDS             complement(14464..15672)
                     /locus_tag="O2692_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ4299468.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
BASE COUNT         4997 a         2802 c         2421 g         5608 t
ORIGIN      
        1 aaatattgaa aaccactaac aaattgcata cacaatatca ttagtggtaa cagttaaaca
       61 cttatttatc tttacgtggt aatggataaa acccttcatt aaattgatgc cataaattag
      121 gagttaagtg atgattatct tctctctctg gatcaaagta attaataatt tcatcttccc
      181 gtccatcttt aattttttca acaaattgat aatccagtag tagctctcta ccaatggcta
      241 ctaagtcaac accaacattt tcaactgcat ctaaagcttc gtcagctgtg aaaattgaac
      301 caataccgat aagtggcata cgaccattta tccatttgtg aattaaaggc agtctttctt
      361 gtccagcgta tttaccttca cgtgtcgttg catgcgtatc catcattgaa acatgaatat
      421 agtcgattgg catatggcta attttattaa cgagttcctc tgtaatttcc attgtgattc
      481 ctggagactc cgcttcctct ggagataatc tgtatccaac tataaagtct ttattgccat
      541 acgcttcttt cgctttaagt acttcttcaa tcacagcgac cgggaattta tattgatttg
      601 cccatacatc atttcttcta ttatagtatg gtgatacaaa ttgatgaatt aagtaatgat
      661 tcgcgccatg tatttcaaca ccatcaaacc ctgcttcaat tgcacgtcgc gttgcttcac
      721 caaaatcctt gattgcttgt tcaatctctt cattcgtcat ttctctagca ctatgttctt
      781 gtttctgacc aaaacttttt aaagaaattg gacttggtgc tacgacgtct ccatcaggtg
      841 ttaattcagg caatgcttgt gcaccgccat gatgtatttg tacgagtgct ttggcaccgt
      901 ttttcttcat tgctgtagct aatcgtttta gtccttcaat attactgtca tgcgcgattg
      961 atggctgtcc tggaaatgct tttccgacat cactcacatt actcgcagca ttaattgtaa
     1021 taccaacatc ttgtgaacgc ttttcaatat aaggaagttc tacatctgaa atagtaccat
     1081 catcatttga agaaatatgt gttaaagggg ctaacacaaa tcgatttctc aactctactc
     1141 catttggtaa ttctacttta tcaaacaatg gttcgtattt acttttcatt acttaattcc
     1201 tccttaaaat tattgagttg actataacat gtaatttttt cattctccaa ttatctgttt
     1261 atatagttgc aattcgttta atactttaag ttgtagttct catttctaaa ataaataaaa
     1321 aaaacaaatc cacatacaat attgcaaatc aacgattcta tctaacaaag ataatggata
     1381 tgtcacctta aaccatttgt acgtgtaaat agaatcaatc atattacgtt atcgtagaca
     1441 aaaagagatt agtatctaat aaaatttaat agatactaat ctcttttata gtctaagaaa
     1501 ctttcatttc agaccctttg tatgattttc ggtacgattt atctgttcct ttagtcgttt
     1561 cgaattgctc gctttgactg ttttatccag attggtaagc gttctttaag tgtttgaaac
     1621 ccatattttt ctgtttctct gatttcatct aatactgatt gtttttcctt cttacgctca
     1681 taatttttga agtaatcatt atcctttaat gatagattaa gctttccttc atcatctata
     1741 gacaaaattt tagctttaac aatttggcct tctgatagaa atttcttcaa attatgaacg
     1801 tagtcatcca taatttctga tatatgaatc agtccttcag tatgattagg ggtctcaaca
     1861 aacgcaccgt atggttgaat accagttaca cgcaccttga tatgttggcc aattttgtag
     1921 ttattcaact taataacccc ttatttcgtt tacttaacac tattatcata gcacaaatcg
     1981 aaatggtgga ctaattaaaa catcaaatca tacgaatatt attttataaa tgaataagaa
     2041 ttctacaaac ttggatttac aaataaaatt agcaacaaat tcctatctaa atcaatatga
     2101 tatccagcta ctttgttttc aatttattta ctatttgaat agcaattctc tgtaaaaaca
     2161 taaaaagcaa cctcaactat tatctttaaa atacacatct atttaaaaac tgtgctttct
     2221 tgataaatat attgagattg ctcatcatat tagtttattt attttcgttg gaatatatac
     2281 taatccattc ataaattagc tttatccgag tttaaaatgt tggtagttaa ttatgtttag
     2341 atattattct tcaacatcaa tagattcaat tacaacatca tgaagtggtt tatcttgtgg
     2401 tcccactttt gtatttgcaa tatcttctaa tgtagtttca ccatcaatga tttgaccgaa
     2461 tactgtatgt ttttgatcta accatggtgt accacccttt tcgccatatg catcaacgat
     2521 tggttgaggc cagccaccat ctgcaagttg acttaacata ttttgaggta cttctttcat
     2581 ttgaacaatg aaaaattgtg aaccattagt attaggtcct gagttagcca ttgataatgc
     2641 gccatataag ttaaatgcat ttaatgaaaa ttcatcttca aaagcaccgc cataaatact
     2701 ttcgccaccc ataccagtag ctgttggatc gccaccttga atcatgaagt cattaatgac
     2761 acggtggaat gtgattccat cataataacc attttttgca tgtgtcacaa aattttcaac
     2821 tgtttttggt gcaatatttg gaaataattt gaatgtcatg tcacctttat ttgtgtgcat
     2881 aaccactttg atttcacctt gttgtacttc tttgtttaac tgtggatagt tagccattta
     2941 tattctccat tcatgttatg atacatatat attaacatac ttttaaggga ggaaaatagc
     3001 atgaagctat atcgattttc acacaaaaca ggtagctatg gtgtcacgat tgttgaagaa
     3061 aatggagacg atgtccttgt taaagttgaa caagttatca aacacccgaa acaaggtgat
     3121 ttacataacc ctactgaaac tgaaggtgta tttttccatg accgcaaagc attaagccat
     3181 tttgaaaaac gctatacgaa acgttcacaa ttacgcgact tcaatgttga tgtattgcgc
     3241 tatgaagatt cacttcaaca agcaattaca gatttagaaa ctaaacttaa agctcagcaa
     3301 acaaaacatg ctgaaatgtc tttagctagt ttagcacgct taaaagaaga ttattcactt
     3361 caatacaaag aaaattttaa ataaaaaatg acgtgtttct tcttatttaa atacttctga
     3421 aaataaagca caacaaggag tgtgtacgtt tttcagaact atatcattca tgtataatga
     3481 ataacataaa tattttttaa aggaggacaa catagtgagt ttgttacata ttgcggtgat
     3541 attaccgtta atattcgcac tcattattcc tatcctgtat agatttttta aacgtataca
     3601 tttaggatgg ttcgtattac ctgtccctat cgtcatattt atttatatgc ttacattaat
     3661 taaaacgacg atgtcaggta atactgtaat gaaaacctta aattggatgc ctcattttgg
     3721 tatgaacttt gatttatatc ttgatggctt aggtttactc tttagtttgc ttatttcagg
     3781 aatcggcagt ttagtagtat tatattcaat cggctactta agtaagtcag aacaactagg
     3841 gaatttttat tgttatttat tactatttat gggtgcaatg ttaggtgtcg tactttcaga
     3901 taatgttatt attctatatt tattctggga gctaacgtca ttttctagtt ttttacttat
     3961 ttcattctgg cgcgaacgac aagcatctat atatggtgct caaaaatcgc tcataatcac
     4021 agtatttggt ggtctatcat tattaggtgg tatcatctta ctcgctatac caacacaatc
     4081 atttagcatt cagtacatga ttcaacatgc ttctgaaatt caaaatagcc cattcttcat
     4141 ttttgcgatg attttaatca tgattggtgc ttttacaaaa tctgctcagt ttccatttta
     4201 tatttggcta ccagatgcaa tggaagcacc tacaccagtc agtgcttatc ttcattcagc
     4261 aacaatggtt aaagcagggt tatatttaat cgctcgaatg acaccaatct tcgcagcatc
     4321 gcaaggttgg gtatggactg tcacactcgt tggtttaatt acattattct gggcttcgct
     4381 aaacgcaaca aaacaacaag acttgaaagg tatactggca ttttcaacag tatcccaatt
     4441 aggtatgatt atggcgatgc ttggaatcgg tgccattagt tatcactatc aaggcgatga
     4501 tagcaaaata tatgctgctg catttacggc tgcaatattc catttaatca accatgcaac
     4561 atttaaaggt gccctattta tgattacagg tgcagtggat cattctactg gtacacgtga
     4621 tgttaagaaa ttaggtggct tactaacaat tatgcctatt tcatttacaa tcactgttat
     4681 tacagcatta agtatggctg gtgtaccacc gttcaatggt ttcttatcaa aagaatcatt
     4741 tttagaaaca acatttacag caagccaagc aaatttattc agtgttgata cattaggcta
     4801 cctattccca atcattggta tagtgggcag tgtcttcaca tttgtatatt caattaaatt
     4861 tataatgcat atatttttcg gacaatataa acctgaacaa ttacctaaaa aagcacacga
     4921 agtatcaata ttaatgcttt tatcaccagc tattttagct acattagtaa ttgtatttgg
     4981 tttattccct ggcattttaa ctaattcgat tattgaacca gctacatctt cgattaatca
     5041 tacggtaatt gatgatgttg aattccatat gttccatgga ttaacgcctg cctttttatc
     5101 aacattagtg atttatattt taggtatctt actaattgtg acatttagtt attgggtgaa
     5161 attgttgcaa cgacaacctg gtaaactaac attcaattat tggtacaaca gaagtgcaaa
     5221 tgttattcca aactactctg aaaagatgac gaatagctat gttaccgatt attcacgtaa
     5281 taatttagtt atcatttttg gtgcattaat tttattaaca tttgtcacta tattctcagt
     5341 acctttcaac attaatttta aagacgttag tccgattaga attttcgaag tttgtatcgt
     5401 aattttatta ttaagtgctg cctttttaat tcttttcgct aaatcaagat tatttagcat
     5461 cattatgtta agtgcagttg gatatgctgt gtctgtattg tttatattct ttaaagcacc
     5521 cgacttagca ttgacacagt ttgtcgttga atctatttca acagcattgt tcttattatg
     5581 cttctaccac ttaccaaatt taaatcgtta taatgaaaag cgctcattcc agcttactaa
     5641 tgctttaatt gctggtggtg ttggtttgtc cgtcattatt atcggtttaa ttgcatacgg
     5701 aaatagacat tttgaatcga tatctaagtt ttatcaagag cacgtttatg atttagctca
     5761 cggtaaaaac atggtaaacg tcatacttgt tgatttccgt ggtatggata ctttattcga
     5821 gtcatctgtg cttggtatcg caggattagc tgtatataca atgattaaat tacgtaaaaa
     5881 gcgtcaaacg caaggtaacg aggtgaaaaa tcatgaatag acaacaaaat gatctaattt
     5941 tgcaatttgc tgcagttatt atctttttca tggtaatggt ctttggtttt tcacttttct
     6001 tagcaggaca ttatacacct ggtggcggtt tcgtcggcgg attattattc gcaagttcac
     6061 ttgttatcat tacgattgca tttgatatag aaacaatgcg taaaattttc ccattagatt
     6121 ttaaaatctt gataggtatc ggattagttt tttgtattgc gacgcctatc gcaagttggt
     6181 ttttaggcaa aaatttcttt acgcatgtca catttgatat accgttattt atattagaac
     6241 ctgttcatat gacaacagca gtattctttg atttcggtgt tttatgtgca gtagttggta
     6301 ctgtcatgac aattattatt tcgattggag agaacgaata gtggaaatta ttatgatttt
     6361 tgttagtggt attctcacag caattagtgt ctatctcgtt ttgtctaaaa gtctgatacg
     6421 aattgttatg ggaactacac tattaacaca tgcagcaaat ttatttttaa taactatggg
     6481 cggacttaaa catggtactg ttccaattta tgaagcgaac gtaaaaagct atgttgatcc
     6541 tatcccgcaa gcacttattt taacagcaat cgttatcgcc tttgcgacaa cagccttttt
     6601 cttagtatta gcatttagaa catataaaga attaggcaca gataacgttg agagtatgaa
     6661 aggagttcca gaagatgatt gaaagtaata tgcttgtgtt aacgctagtt attccagtaa
     6721 ttacagccat attacttgtc tttattggaa agcgtcctat tataaagcgt tatgtcgcac
     6781 taggcggtac attattaacc ttggtcgcgg caattatcaa tttagcaaat gttgttaaac
     6841 atggaccaat acgcgtagag ctaggttcat ggaaagcacc atacagtatt gtctttgtat
     6901 tagacatatt cagtgcccta ctaataatta ctagtattat tattacagct attgtgattt
     6961 tatattcata ccaaacaatt ggtattgaac gtgaaagata ttattattat ttttcagtac
     7021 tatttatgtt aataggtatc attggtgcat tcacaactgg tgacattttc aacctattcg
     7081 tatatttcga agtattttta atgtcatctt atttcttatt agtaattggt tctacaaaaa
     7141 ttcaactaca agaaacaata aaatatgttc ttgttaacgt tgtatcgtca tccttcttcg
     7201 taatgggtgt agcgatttta tattcagtag taggaacatt gaatctagca gatataagca
     7261 ataaattagc taacctttca gcgcatgaca gtggcttagt caatatcgtt ttcatactat
     7321 ttatctttgt ttttgctaca aaagctggcg tattcccaat gtttgtttgg ttacctagtg
     7381 catactatgc accaccaatt ccaatcattg cattctttgg tgctttgtta acaaaagttg
     7441 gtgtttatgc aatcgctaga acattgagcc tattctttag tgataatgta agcttttcac
     7501 attatgtcat tttattctta gcactattaa cgatcatctt tggttgtgtc ggtgctgttg
     7561 catatgccaa cattaaaaaa atcatcctat acaatgtcat gattgctgta ggtgtgatct
     7621 tagtcggcgt tgcaatgatg acagagtcag gtatgattgg ggctatttac tatacattgc
     7681 acgacatgtt agtaaaactt gcattattct tgcttatcgg aattatgatt aaaattacag
     7741 gcactgctga tttaagacaa tttggtggct taattaaacg atatcctgta cttggttggt
     7801 cattctttat cgcagcatta agtttagctg gcattccccc actcagtggc ttttatggaa
     7861 aattttttat tgtccaatca acatttgaaa gaggatttta tttaagtggt gtcatcgtct
     7921 tattatcaag tttagtcgta ttatattctg tcattcgcat attcctacaa ggctttttcg
     7981 gtcaaccaaa aggatatgat cttaataaca aagtcgacgt caaatattta actacgattg
     8041 caatcgttgc agttgtgata actgttttat atggattgtc agcagattac ttatatccaa
     8101 tggtaaaagc tggtgcagaa acattctaca atccatcaac ctacgttaaa gctgtattag
     8161 gaggtaaata acatggctgt acaattagta ttaaacttta ttatcgcagt gttttggttg
     8221 tttgtgacaa atagttacac aacaaataac tttgtgttag gtttcatctt tggcctagtt
     8281 cttgtttacc tcttacacag agtgttacca ggtagatttt atgtcatcac gctatatcgt
     8341 attattaaat tagttattat tttcttaatc gaactgatta aagctaattt tgatgttttg
     8401 aaaattatta ttaaaccttc gattaaaaat gaaccaggat tctttgttta tcacacagat
     8461 ttaaaaaaag attggcaaat tgtcttgctg tctaatttaa ttacattaac acctggtact
     8521 gtagtgcttg gtgtaagtga cgatcgtaca aaaatttata ttcatgccat agatttttca
     8581 actaaagagc aagaagtaga aagtattaaa acatcattag agaaaattgt aagagaggtg
     8641 ggcgaaatat aatgaatcat aatgttatta tcgttattgc attaatcata gttgtcattt
     8701 ctatgttagc tatgctcatt cgcgttgtgc taggcccatc acttgccgat cgtgttgtcg
     8761 cattagatgc gattggtctt caattaatgg cagttatagc attattcagt attttattaa
     8821 atattaaata catgattgtc gttattatga tgattggtat attagctttt ttaggtactg
     8881 cagtattctc taaatttatg gacaaaggta aggtgattga acatgatcaa aatcatactg
     8941 attagtcttg cacttatctt tgttatcatc ggtgctttaa ttagcgccct agcagctata
     9001 ggattattga gacttgaaga tgtatattca cgtgcacatg ctgccggaaa agcatcaaca
     9061 ttaggtgcaa tgtcattact atttggtacg tttttatatt ttattgctac tcaaggtttt
     9121 gtaaatatgc aattaatcgt tgcgattatc tttgtattaa ttacaggtcc tctttcaagt
     9181 catatgatta tgaaagcagc atataatatt aaaacgcctt atactaaaaa gactaaagtc
     9241 gatgaaattt cggaagactt aaaagacaca aaattgtaga ttatataaca aaatcatgaa
     9301 tataaatcaa caacaaacag cagtaagatg attccaaatt aggaatgatt ttactgctgt
     9361 tttcttttga cattgttacc tctttttcaa tgattttttc tttgactaca gattcgccct
     9421 atctacatat atctctttaa tttaattgcc tttcatgtcg ttatgtatta tgataataat
     9481 aattataaat cgtaacgatt acgttttaaa aagagagagg ttttattatg cattggacaa
     9541 ttatcggcgg tggcatacag ggaactgcaa tcgcacaaaa actattatca agcggattaa
     9601 caacagaccg attaacaatc attgacccac acgaaacttt ttgccaaagg tttaactcat
     9661 atacaaatcg aatagaaatg ccttatttaa gatcaccgat tgtacatcac gtacatccac
     9721 aaccgttcca tctaaaacaa ttcgctaaac agcaccaata tacaaatgct ttttatggtc
     9781 cttatcaacg acctgaattg acaatgttta tggatcatat tgcacatgct tctaaacaat
     9841 atcaattaga ggattgcttg gttcaaggtt tagttcaaac tttagataaa caagaagaca
     9901 aatggcatat caagttagaa gatggacaaa ttatcactac agattgcgtc gttattgcaa
     9961 taggcagtac aaatattccg tttatgcctg acattttaaa agacaaacag aatgtaaatc
    10021 atatcttcga gaaagaactt gatcaagtag tatatgataa gaccgatcat atcgttggta
    10081 gcggcattac tgctgcacat cttgcactta aattgttaaa tcatgataac gataaaaaga
    10141 ttcatttatg gctaaataaa gatattgaaa tacatgactt tgatgctgat cctggttggt
    10201 taggtccgaa aaatatgtct tcatttttaa gtactaaaag catgcctgaa agaaatgcca
    10261 ttgtacaacg cgaacgtcat aaaggatcaa tgcctcacga actgtactta cgccttaaaa
    10321 aacatattaa aaatggtcgt ataaatgtgc ataaaacacc tatcactcaa attagtggtg
    10381 gtgtaattaa cactgaaaat gattctgttc catatcaaca gattatggtt gcaactggtt
    10441 ttgaacaaga ttttatgtca caaccactta ttaagcaatt aatacaaaat tatgatgcac
    10501 ctatcaacga atgtaattac cctgttattt ccgaaaaatt agaatggata ccaaatctat
    10561 ttgtcgcagg atgctttgca gacttagaat taggaccatt tggtagaaat gttatgggtg
    10621 gccgtaaagc tgccgaacgc attgaacaag catttctaaa actacaacaa tatagcgcat
    10681 aaaaaaactg ctagccgtag ttatgaatat ctcataacat ttcagctagc agttctttta
    10741 ttttaaaggt ttaatagcaa ctgtaccacg cataactgta attaattgtt ctttgtcatt
    10801 cttaattttt atatcccata catgtgtcga cttacctcga tgaataattt cagctgtcgc
    10861 agtaacacga ccatctttag cagaatgaat atggttagca ttcatctcta aacctaatgg
    10921 aataaatttg gttgtatcaa ttaaattagc agatcctaat gaacatgctg tttcacctaa
    10981 agcaatcgaa gccccaccat gtaaatatcc aaatggttgt tttactttat cagtaacagg
    11041 catagaaata acaactaaac catcttcctg gtgatctatt gacatctcaa atgtctctaa
    11101 taaatgagtc atattttcac ctctttatgc ttatagtacc atagcagcaa tagtaccgaa
    11161 aatcattaaa ggaatattat aaaacaagaa gtttggtaca catgtatcac gtatatgatc
    11221 atgttggcca tcaacattta atcccgcagt tggtcctaat gttgaatcac ttgcaggcga
    11281 acctgagtca cctaatgcac tcgctgttcc aatcaatgcg attaatgcca ttgtatctag
    11341 tccaattgac gctccaaaag gaatgaataa tgatgcgata ataggaattg ttgcaaatga
    11401 tgatccaata cctaaagtga caattaaacc tatcacatac atcatgataa tgctaaataa
    11461 tttattatca ccagtaatac ttgttaaagt tttaactaat tcatctatat caccagtagc
    11521 attcattaca ccagcaaatc catttgctgt taaaataact acaccaatat aagccataat
    11581 tttaatacct tcaacaaact tagcatctaa ttcataccaa ttatatgcac gtgaaataaa
    11641 gaatacgagt acccctgcca gtgcaccaaa aatcattgaa tctgtaaatg tttgtactaa
    11701 aaatgtagct agtattgcta caattgttac tattaagata tatggtttta actctgtaac
    11761 attgtcacta tctgaaattt tacgtgtttc atattcacgt ggtttacgat atacatataa
    11821 accgataagt aagccaacaa tataccccat tgaaggaata agcattgctt tccaaatcat
    11881 attaaactca attgggtgat ttgcctttgc aaagccactt tgaataattt gctggaaaat
    11941 ttgaccgaat ccatatggta ataacacata cgggaaacat aaaccaaaac cgataatcaa
    12001 accgattaaa cgtctatcta tttttaagtc attaaacaga cttaacaatg gtgggatgac
    12061 aattggaatg aatgcaatat gtacaggaat taagttttga ctcatgatac tcatagctaa
    12121 taatgcaatg attattgtca ctttgacttt aactcttgac catcgactat tttcagcgtg
    12181 aattgcattt ataatttttc ctactaaata gtctgtgata ccactgtatg aaattaatgc
    12241 tgcaaatcca cctaataaag catagcttaa tgctacctca gcaccatcga ctatattttt
    12301 cccaaataca tttataactt tttcaatgct catgcctgaa attaagccac caactagcgc
    12361 actgataaat aagcttataa ctacgtttaa tcgacataaa catagcacaa tcattaaaat
    12421 tactgctatt actactgcat ttatcatatg acaaccatcc tttatcactc tatcgaacta
    12481 aagcgtttat agactacata gtatcagtag ctttaacacc tgtcaatagc aaactatacc
    12541 aataatcttt attttttagt taattgtctt tcttaaaata attttagctt tcattaaatt
    12601 aaacaatttt acaagcttgg aacaccaatc aaaatcctaa gttctaaaat gcaatattag
    12661 tagtcgttga ctgaatgaac atatgcttat aatatttttt tgcaatgcta gtcaagttga
    12721 tttatgctca caaggatatg cgatttatat tttcttacaa caatgaaaat gcctgataca
    12781 atgcgatcct ttatactttc aagagttaaa ttagaaatgt atatcaagac acaagttgta
    12841 tcaggcaaat aattaaatta ttgttgtttt aaccattgta ctatcgtcgg aatcataaat
    12901 cctgttggcc cttttggacc attatatgaa gctttattag tcgttgctgg acctgcaata
    12961 tcgaaatgaa tgtgaggtgt ttgaccacta aaatgtgtta caaaacttgc cgcaaatagc
    13021 gctttacctt gtccattcgt atggttaact aaatcagcga tatcactgtg tttaatactt
    13081 gcacgttcgg ttgcagtaat cggtaattca aataccattt catcgacttc agaacttatt
    13141 tgtaatatat cgtttaatat cactttacta ttcgattcaa atgcagcagc tttatcatcg
    13201 cctagtgcaa caattgctgc acccgttaat gtagcaaagt ccataatcac actaggctga
    13261 tattgatttg cataaaacac agcatctgca aggactaatc taccttcagc gtctgtattc
    13321 attacttcta cagtttcacc acttaatgct gtaaatacat catctggctt cattgatgct
    13381 tcatttatca tattttcagc acacgcaagc actccgacaa tatttacagg cagttgtaaa
    13441 cgactagccg cttcaatgat accaacgaca ttcgcagcgc cacacatgtc aaacttcatt
    13501 gtagccatgc cattcttcgt tttaatacta taaccaccag aatcatacgt tataccttta
    13561 ccaactaagg caattggtgc ttcatctttg tctttgccat tatatgtgat ggttactaat
    13621 ctcggtttat gcttactacc tttacctact gcttgtaaaa gtccgaatcc ttcagaaact
    13681 aaagtgtcat aatctttaac atctactttg acctttgtat ttttaaaatg attaacaata
    13741 tcttctgcaa atgtttgtgg tgttaataca ttcggtggca tattactaaa gtctcttgcc
    13801 aaattaatgg attggccaat actgataccc tcatgaataa aatctaattc aattaatgat
    13861 tcactaataa ggtttaaatt cgtcttaaac ggtgccttct tacttgattt ataatgatcg
    13921 aattcatatg ttgcacgctc actttgaata ccgcatgcca ttaatacatc tgataactga
    13981 tcatattttg aaataaatga atccataagt aaatacgtat cttcaatgtg ttctgacttt
    14041 atgtattgga aaagatgtcc ccatattttc aacatatctt gataactacg tgtctttaag
    14101 tttcctaaac caactgtaat taaacgatat gtttgatctt gtacatcaaa tgcagttgta
    14161 taaatcttcc caactttact accaataata tgttgatgtt ttagtctttc aagtgattct
    14221 gtaatatcga tatgattaaa actaatgcgc tctaactgat ttaaatgttc tggtatacca
    14281 ataatcaatg tatttatttc gttgcttagt gtgttattta atttaaaatt cataatgtac
    14341 ctccttagat ttgatatgtt actcacttca aattgtacaa caataaagcc cttcagtgac
    14401 actgaaggac tttataaatg aataatttaa ttttatgtgt ttagctgaaa ctttatttaa
    14461 agcctagaat ttaccttttt tgaatgctaa accgatacca ccgattttga ataccgcacg
    14521 tgtatcaatc acttttttca tgaatgctgc ttttttacca gcgataggtt taccaaatac
    14581 catacctaca ccgtcatgtg aacctaaaga acaaacagtt ccacgatcaa cgtattcgaa
    14641 ttcttcagtt gattcaccgt ttaagatgcg tttaatgttt ttagcaacac tttcaccttg
    14701 ttgcattgca atttgtgctg tagttggtaa tggacgttct tctccagctg ggataaacgc
    14761 tgaacagtca ccaataacaa aaatgttgtc gtaaccattg attgttaaat cttgctttgt
    14821 aacgatacgt ccacgtttaa cgccttcaaa tgattcttcc attaatttac taccacgtac
    14881 accagctgcc catactgaag tacctgcatt taattgttgt ttttcaccat ctacttcaac
    14941 tacaaaacct ttttcgttac aagcaacgat tggtgtagca attttaaatt caacaccgcg
    15001 gtcttctaag tagctaactg cgtggttaac taattcttct gagaacattg gtaacatttt
    15061 aggtgctgct tcaacacaag tgattttaac tttattttga tccacaccat atttgctaca
    15121 taattcagga attctgtctg ttaattcacc taagaattca acaccagtga atccagcacc
    15181 accaactaag atagataaat cgttatcatc tttttctttt gatgctgcat agttagcaaa
    15241 tttgtcttcg atatgacgtg ataattcacg tgctgtgata acattttcaa tttggaaagc
    15301 atgatctttc ataccttcga tgccgaatgt ttcactaacg aaacctaatg ctactactaa
    15361 aatatcaaag tcataaatac cttgatttgt ttctaccttt ttagcatcac ggtcaatttt
    15421 tgttacttct gcttgaacaa agttcacttt gtctttcttc aagacacttt ccacaggata
    15481 taatacatct tcatagttta gtgtacctgc tgatgcttca tgtaaccatg ttgcttcata
    15541 gtgatattca tttttattaa taagcgtaat ttctgcttct tctgttgata tcgctttttg
    15601 caatttagtt acagtttgta aacctgcata accagcacca agtacaagta cttttttacg
    15661 atcttgagcc atttaatttc acctaagctt tcatattttt ttaaccaaat gctgataatt
    15721 actagtaaca actggtcatt actactttta ccacaatgta tactagttta tcatcatttt
    15781 gttatttaag tcttttaaat tatgtaaaaa cctgtgatgt tcacaaaa
//
LOCUS       DALAGF010000067        15826 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.067, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000067 DALAGF010000000
VERSION     DALAGF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15826
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.067"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(208..1500)
                     /gene="asnS"
                     /locus_tag="O2692_001844"
     CDS             complement(208..1500)
                     /gene="asnS"
                     /locus_tag="O2692_001844"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCZ4299469.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            complement(1822..4515)
                     /locus_tag="O2692_001845"
     CDS             complement(1822..4515)
                     /locus_tag="O2692_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase DinG"
                     /protein_id="HCZ4299470.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            complement(4539..5510)
                     /locus_tag="O2692_001846"
     CDS             complement(4539..5510)
                     /locus_tag="O2692_001846"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCZ4299471.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSIQLHDKNKLDRYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            complement(5497..6699)
                     /locus_tag="O2692_001847"
     CDS             complement(5497..6699)
                     /locus_tag="O2692_001847"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCZ4299472.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            complement(6704..7846)
                     /gene="bshA"
                     /locus_tag="O2692_001848"
     CDS             complement(6704..7846)
                     /gene="bshA"
                     /locus_tag="O2692_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HCZ4299473.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            complement(8087..8404)
                     /locus_tag="O2692_001849"
     CDS             complement(8087..8404)
                     /locus_tag="O2692_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCZ4299474.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(8741..9421)
                     /locus_tag="O2692_001850"
     CDS             complement(8741..9421)
                     /locus_tag="O2692_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCZ4299475.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
     gene            complement(9493..10080)
                     /locus_tag="O2692_001851"
     CDS             complement(9493..10080)
                     /locus_tag="O2692_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HCZ4299476.1"
                     /translation="MTIKAFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILYLVISISLILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMIWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            complement(10070..10645)
                     /locus_tag="O2692_001852"
     CDS             complement(10070..10645)
                     /locus_tag="O2692_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCZ4299477.1"
                     /translation="MSETFNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDN"
     gene            complement(10659..11903)
                     /locus_tag="O2692_001853"
     CDS             complement(10659..11903)
                     /locus_tag="O2692_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299478.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            complement(11910..13208)
                     /gene="aroA"
                     /locus_tag="O2692_001854"
     CDS             complement(11910..13208)
                     /gene="aroA"
                     /locus_tag="O2692_001854"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCZ4299479.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            complement(13218..14282)
                     /locus_tag="O2692_001855"
     CDS             complement(13218..14282)
                     /locus_tag="O2692_001855"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCZ4299480.1"
                     /translation="MKLQTTYPSNNYPIYVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFIAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVIADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            complement(14308..15474)
                     /gene="aroC"
                     /locus_tag="O2692_001856"
     CDS             complement(14308..15474)
                     /gene="aroC"
                     /locus_tag="O2692_001856"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCZ4299481.1"
                     /translation="MRYLTSGESHGPQLTVIVEGVPANIEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDSIAQAMRDKIDEAKNEGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGKIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNSEIGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
BASE COUNT         5046 a         2912 c         2121 g         5747 t
ORIGIN      
        1 ttaacactag attttaacaa tgaactttat ggataatcag atgaactatt tattttccaa
       61 atatagttac taaacaaaag tttgttcatg attaatataa gccgttatca atcttcattt
      121 tccaagaata aatcgcccag ctattaatat aggctggacg attcttttta ttatcataat
      181 atatatttta agtaacgacc ctaaaactta tggatataaa cggtttaata atcttgggaa
      241 tggcgctgtt tcacgaacgt gttcaacacc agaaatccat gctactgtac gctctaaacc
      301 taaaccaaat ccacagtgtg gcacactacc ataacgacgt aagtctaagt agtaactata
      361 tgcttcttcg tctaatccat gttctttaac gcgttgttct aacaattcta agtcatccac
      421 acgttcagat ccaccaataa tttcaccgta tccttcaggt gcaattaagt ctgcacataa
      481 tacagtttct tcattttcag gatttggttg catatagaaa ggcttaattt tagttggata
      541 attagtaata aacaccggta aatcataatg attagcaatg gctgtttcat gtggcgcacc
      601 aaaatcttcg ccccattcaa tatcatcaaa gccttctgct tttaagaatt caattgcatc
      661 atcatatgaa attctaggga atggtgtcgc aactttttca agttttgatg tatcacgctc
      721 taaaattttc aactctagtt tacaattttc taaaactgat tttacaacat gtgttacata
      781 ttgttcttga atttctaaac tttcagcatg atttgtgaaa gccatttctc cttcaatcat
      841 ccagaactcg atcaagtgtc tacgtgtttt tgatttttca gctctgaaag ttggaccaaa
      901 tgaaaatact tttccgtgtg ccattgctgc agcttctaag tataactgac cactttgaga
      961 taaaaacgca tcttgatcaa agtatttagt atggaataat tcacttgtac cttctggcgc
     1021 acttgctgtc aaaattggtg gatcaacctt tgtaaatcca tctttgttga aaaattcata
     1081 cgttgcacga ataacttcat ttctaatttt cattacagca tgttgttttt tagaacgtaa
     1141 ccataaatga cggtgatcca ttaagaattc tgtaccatga tttttaggtg taatcggata
     1201 gtcatgcgct tctgaaataa cttcaattga tttcacttgc atttcgtatc ctaagtcaga
     1261 acgattatct tctgtaattg tgcctgtaac gtatagagat gattcttgag taatttcttt
     1321 cgcaagtttg aatacctctt catcaacttc tgatttaact actacgcctt gcataaagcc
     1381 tgttccatca cgtaattgta aaaaggcgat tttaccactt gaacgtttat ttgttaacca
     1441 agcaccaatt gtaacgtctt ggtttaaatg atctttcgct tgtttaatcg ttgttttcat
     1501 aaccattctc ctatttattt tttcgttata caatactcat tcattttaac aaaatccgct
     1561 ttcaagttct agaactagac taaaagataa cgtgtaaatg gtaatgattt acgcacagat
     1621 ttgaacatta atttatatta agacaataca atcatcagtt tcagacaaaa tatataataa
     1681 agcctcaata ttattagtat gaaggtcgtt ttttgtgcta acattcaagt ttaattataa
     1741 aactcaaatt ttgacgacca aacaaaaatt cttgtaacag aacatagcac atgtcatgtc
     1801 acaagaattt tactttgtat ttcacttttt ctttttttga atttgtctta ataattttcc
     1861 aaactgttga atgtcgcctt ttttctgacg ataattttca agtgtttgtt caaaaaagtt
     1921 tttataatta ctgtttatga gtcgatcatc aaatgaaact attatgccgc gatcattttc
     1981 atttctaatt aatcttccaa gtccttgtct aaaacgtgta actgcatcag gtaatacata
     2041 ttccttgaaa gttgaagtga attcagaatc cataagccaa tattttgcat tatgcttgtt
     2101 cataaacggt aacttcgcta tcatcacaca tttaatacca tttgcttgaa aatcaaaacc
     2161 ttcaaaaaat gttgacgtac caagcagtat ggccttatca aaattattaa actgttgtac
     2221 tattttataa ttttggttct gctgttgtgt taatacaaca taatcttcaa attctggcaa
     2281 ttcatttagc atatcttgta ccatatgcat cattttataa ctcgtaaata agactaaaca
     2341 ttttgatgac gttatagtcg tatattcaat aatatagctt acaatcgatg ctacatactc
     2401 atctatattt ttatattgat aagatgctac atcactcggt ataaatacac ttgtattttt
     2461 tgcactttgt aacgacgtgt taacttcaaa tgtattaaag tgaacatctt tgttgaataa
     2521 ctgtttaaaa gcttcaaacg aatgattaaa ttttaaagta ccagatataa aaatgagcga
     2581 tttaaatttt tccaatactt gtttcgttaa tacatctttt acagcgtaat ctttcacata
     2641 taaacgtatt gttgattttt gagataaatt tttaatcgaa atgaaactag tatgtccagc
     2701 ttttaaactt tgttcgatat ttttaaattt atcttttaaa tacaacaact gtttccgtaa
     2761 tgatttaact gttttatgac taatgccatt gaatatttct agcgttttat ttaacttatc
     2821 gataatcgca tgtaaatcct tcaaaatgtc ttttgtttca aaagtaaata cattatggaa
     2881 gcgatgaata tcatcatcat aaacatcaga atcattgata atcgtaaata tcgttgagaa
     2941 caattgctca tttaactcat gaatctcatt catactagcc ttcaagccaa aaatatcaat
     3001 tggtgcaata tctaattttt ccaaaattcg ctgcttttcc agttgatcaa ttgcctttaa
     3061 caatttttca ttttcgtttt taccaatcaa accaagctga tatttaatat cagcataact
     3121 caactcattt gtcacttgat ttaaggcata gtctggtaag cgatgtgctt catccactat
     3181 acaatcatca aacaattgat atattgaatt ttcaacatca gaatgaatta aatgtgcatg
     3241 atttgtaata ccaatttgaa tgttctgtgc atttcgctta ataaaattat aataatgaac
     3301 atcgtgacgt gccggtacat atgtttcaat tttctggtca aaatacatct tttgaccacc
     3361 ttttaaattt aattcctgta tatctccgga cggcgtctct gtaatccaaa tcagcaattg
     3421 cattttcaag atattcactt cgtaattact tgtgtcatct tttaaaattt gactaataag
     3481 ccccaatgaa atgtaatcac ttttactttt aatcaatagt gcattaattt taaaattcaa
     3541 cgcttcattc attgctggaa tatctttttc taacaattga ctttgcagta atttagtatt
     3601 ggtagaaatc atgacatgct tcccagtttc aatattatac atcaaggccg caagtaaata
     3661 tgctaatgat ttaccactgc ctagtgatgc ttcaatcatt gctttttcac tatgcatgag
     3721 ctgatctaat atagtttccg ctaaatataa ttgttgcggt cgatatgtta agccaagttg
     3781 atctacagct ttgctatata aagacttcaa gctgccatta taatttgttg tcggcttttt
     3841 aaaatcaact tgcttacgat agataatctg ttcgaacttt tcgtacgatt tatccaatgg
     3901 ctttgcatca tattgcctaa ccatctcaaa gaaaatatca tacaaatcgt atttcaactg
     3961 tttacttaaa taatataatt gcttcaaagt atctaacggt aacttttcaa atttttcaaa
     4021 agctaatatc atcaatttag cagtagtagc ggcatcttcg tcagctcgat gggcatttgc
     4081 taaggtaata ccatgtgcct ctgctaattc acttaattga tagcttttat ctgtaggaaa
     4141 agctatttta aagatttcta gtgtatctat aacttttttg ggacgatatt gaatattaca
     4201 atctttaaat gcctttttaa taaaattcaa atcaaaatct acattatgag ctacaaaaat
     4261 gcaatctttt atcttatcgt agatttcttg tgcaacttga ttaaaatatg gcgcttgttg
     4321 tagcatattt tcttcaatgg atgttaacgc ttgaatgaac ggcggaatct ctaaatttgt
     4381 tctaatcata gaatgatatg tatcaataat ttggttattg cgcacaaacg ttataccaat
     4441 ttgaatgata tcgtcaaaat ctaattggtt gcctgttgtt tccaaatcca caacggcata
     4501 ggttgccata cccatagcta tctctccttg ctttagtgtt aaaaatctat atctgcacta
     4561 attaaacggt gtgattcacc cgcttcatct ctaacaatta gatagccatc gtaatctaaa
     4621 tcaattgctt gtcctttaaa ctgtttatca ttttctgtaa atagcaacgt tctattccaa
     4681 atattagaag ctgcagtata ttcttcacga atttcagaaa aaggtaacgt taaaaattga
     4741 ttatatcttt tttcaatttc ttgaagtaat atctctaaaa attgatatct atctaattta
     4801 tttttatcat gtaattgtat acttgttgct ctatgtctaa tactttcatc aaagttttct
     4861 agttgttgcg tcaaattaat acctatacca catattattg cttctatacc atcattatta
     4921 gcaaccattt cagttaagaa accacacact ttaccattat caatatatat atcattcggc
     4981 catttcactt tgacttcatc ttgactaaaa tgttgaatcg catctcttat ccctaatgca
     5041 ataaataaat taaatttaga tatcattgag aatgcaacgt taggtcttaa cacgacagac
     5101 atccaaagtc cttgcccttt tgaagaactc caatgtctat taaatcgccc acgacctttc
     5161 gtttgttcat cactcaagat aaaaaatgaa gattgatttc caacaagtga ctttttcgca
     5221 gcaagttgtg tagaatctat tgaatcgtat acttcactaa aatcaaacaa agcagaactt
     5281 tttgtatatt ggtctattat accttgatac caaatatctg ggagctgttg taataaatgc
     5341 cctttatgat ttactgaatc tattttacat ccctctaact ttaattggtc aatcactttt
     5401 tttactgcag tgcgtgaaat attaagtgat tccgcaatgc tttgtccaga tatataattc
     5461 ggtttatttt tatagagtaa ttgaagtaca tcttgactat attttgacat gattatccac
     5521 ccatttcaaa atttcagttt cttcgttgct tactttacct gttacaatcg ctatctcaat
     5581 ttgtcttagc acatctttta accacggacc acttttggca tttaaatgtg ccataagtac
     5641 accgccatta accatcatgt cttttctatt atgcataggt aaacgatgta atgtttcatc
     5701 aatcgtttga aggttaacga ttaatggttc atgtccttga atatcatttg cctttaacac
     5761 gtctgctgca accattacat ttttaatgag atgcgtatca taatcataaa caaacatttt
     5821 taattgttct tttgtaataa tacttggtaa tgcattcata atttgaatat attgattgat
     5881 atcttttact tgtcggttac ttagctttaa aggcttcaat gagtaattaa tatcaaattt
     5941 aactgatact atagcaatca acaattctaa atcaattgct tcagttacat taatttgatt
     6001 catatcaaga tgttcgaaat acggcatata attaaatgct ttcagcgatt ttaaatgatt
     6061 aaaactcttt tcaacattaa tacctcgcat taatttagtt agttcaatca ctatacgctc
     6121 aattgataaa aatttaatat ctgccatttg tatacgcatc gcttcgaatg tttccgttgc
     6181 aatatcaaat gataattgtg actggaacct taaacatcga atcatacgta aagcatcttc
     6241 ttggaatcgt tcctcagcta tacctacagt tcttattatt cgattattaa tatcttgttg
     6301 accatcaaaa taatcataca atttgtatgc tgtatccatt gctatcgcat tcatcgtgaa
     6361 atctcgtcgt tgcaaatctt cgtataaatc acgaacaaat gtaacaccac ttggtctacg
     6421 gtgatcgaca taatcttctt cagcccggaa tgttgtcact tcataatttt catcattaaa
     6481 aactacattt atcgtgccat gttctttacc tacaggtatc gtatgactaa agatagattc
     6541 tatttcatcc ggcgttgcac ttgttgtgat atctatatca tgaatatttc ttcccatgac
     6601 ataatctctt acagagccac ctacataata tgcttcaaaa ccattgtctt gaatttgttc
     6661 taatataggc cttgcctgtt caaataatga tttatccata ttattactcg cctttacttt
     6721 tgttatgctc atttagcatt ttttgataat aatactcata ttgatctgta ataagttctg
     6781 atccaaaacg ttcagcaata tctgctagca tgtttttctg aagtttgttg tataacacct
     6841 tatcttcaag taatcggata gcatagtcac tcgctgaatc acaatcaccc acatctacga
     6901 caaatccagt ttcaccatgt ttaataacct ctttaattcc accggcattt gaaccaattg
     6961 gaacgacgcc tgttttcata gcctcaagta aagttagtcc aaagctttct ttttcactta
     7021 ataataatac taagtcagat aattggtaaa attcacttac gcaatcttgt ttccctaaaa
     7081 ataaaacatc ctcttctacg tttaactctt tcgtcaattg acgcattggc actaattcag
     7141 gaccatctcc aagtaaaatt aatttactag gtatcttttc acgtactttt gcaaatgttt
     7201 ctataatagt atctatgcgt tttacttgtc taaaattcga tacatgtatt aacacttttt
     7261 catctggtgc tataccaaat tgtgatttta atgctgtgtt atgtttagtt ggaaactcat
     7321 tttcacgtac aaaattataa atcggtataa tttctttgtt agtttcaata atttcatgtg
     7381 tttcttgtgc taaagattta ctcacacttg tcacaatatc acttttttca atgccaaatt
     7441 taattgcacc ttggagtgaa tgatcatagc ccaaaacagt aatatcagta ccgtgtagcg
     7501 ttgtcataat ttttatatct ttacctgaca tctcacgagc taaaatccca caaattgcat
     7561 gaggtacagc atagtgcata tgcaacaaat caagatcata ttctttaata acttcagcga
     7621 ttttagtact taacgtaata tcatacggtg gatactgaaa tactgcatat tgattcactt
     7681 caacttgatg aaaaatcata ttcggtaatg gttttcttat tctaaacggg atatttgaag
     7741 tgataaaatg tacttcgtga cctcgctctg ctaatttaat tcctaattct gtggcaataa
     7801 ttccagaacc acccatggac gggtaacatg ttatacctat cttcattcgc ttgcccatcc
     7861 tttctttcta tttctctcta ttattctcga tgcgtagata attgtttaaa tttaagttta
     7921 tagtaatgtt gagtttataa tttcatatat ctaaaaacag gtgttgtata tataatcatt
     7981 catctagtta tacttacttt aaaaataata taatttcatg cgatgcaatt cattgatgga
     8041 tgtttttaat cttaatcaaa tccaaataaa gcatatattt ttaaattcac tttctttcga
     8101 atcgattttt atctcttgta ttaaactttt ccattgtttc attaaagctc tctgtcatat
     8161 ctattcccat tgaattcgct aaacataaca acacaaataa attatcacct aattctgctt
     8221 taatcgtatt tgcttcctct gaatctttct tctttttttc accataggta tgatttattt
     8281 cacgtgcaag ttcgcccact tcttcagtca atctagctaa gttagctaat ggtgaaaaat
     8341 atcctgtttt aaattgtcca atatattcat caacttcacg ttgcatttct accattgatt
     8401 tcatttctac gttctcctta tattgcattt ctaatatagt atatatcaat ttgaagtctc
     8461 atgcatgttt agtgcaatct actgactcta gctaaactag atgacaagtt gtttatttta
     8521 gaagtttaat aattcagttt atataaatgt aatgcattcc taactaaatt aaatcaattg
     8581 aaattgggat tataacttta tgatacgtac cactacaata aaataatata gtgaataatc
     8641 taccattaga aaaataagca caaaaaaact agcaaccaca caaaaatgtg attagctagt
     8701 taataagtgt ctaatttaag ttaattgtta atctataaga ttaatcactt gaacgcgcaa
     8761 tcaaaataat acgtacaagc tctgctacag cgactgcagt tgctgcaaca taagtcattg
     8821 ctgctgcaga taatacttta cgcgcatgct tgtattcttt ttcatttaca atgttcaatg
     8881 ccgtaatttg tttcatcgct cttgaactcg catcaaactc aactggtaac gtaacaattg
     8941 agaataatac cgctaatgac attaaaccag caccaatcca taaagcagtt gaaccaaatg
     9001 cactacctat cgctgttaag ataataccta acatgatgat catataactt aatgaactcc
     9061 ctaggtttgc aacaggtact aatgctgctc tgaatcttaa gaaccaatat ccttggtgat
     9121 cttgaatggc atgaccaact tcgtgggctg caattgcagt tccagcaact gatggtctgt
     9181 catagtttgc aggagatagt gaaacaactt tctttttagg atcgtaatga tctgttaaga
     9241 atccttcacc tttaacaact tcgacatcat aaataccgtt tgcatgtaaa atttctaatg
     9301 caacttcacg acccgtttta ccactagttg atctaacttg tgaatatttc tcatagttag
     9361 atttaacttt gtgttgtgcc cataaaggaa gcaccattaa tattacgaaa taaattatca
     9421 tagtaaaaat tgaagacaat aaactcactc tcctttataa atattttact gtcatttgcc
     9481 gtttttatca aatcatttac actttaataa tttgtttaat tcaatataaa gcaaaagtcc
     9541 aaaaacactt agacaacatg ataatacacc aatttgccac acatgtgtag ttataaaatc
     9601 ataatatgga aattgaaggt gaaaatagtc aatataatca ttcaaaaaca cccaaatcat
     9661 cgctacactg attccaatca tagaacgttt aaacctagga tagaagtaaa ttgcctgaac
     9721 agccattata ctgtgggaaa acattaatac caaaccattt actgtaatat caccttgttc
     9781 aataataaat aatatattca ttataactgc ccaaatccca tatttgaata atgttacaaa
     9841 tgccagtgca tcgataatac tattttgttt ttgaattaat atcaatgaga tagaaataac
     9901 taagtataat attgcagttg ggctatctgg aacaaaaatc ttaaaatgcc agggcgtatg
     9961 acttaattgt tcaccatacc atatataacc ataaatcatc cctaatatat tacaaatgag
    10021 tagcatcatt aaccaagaac gttgataaag tgtatattgc caaaatgctt taattgtcat
    10081 ctgctaagtc ctcaaattga ttatgtttat ttactagctt gagtgtattt aaaatttgcg
    10141 ttagttgata aaaacgttgc ttttcattca tctgtaaact taaatcaata ttgtgtaaca
    10201 agtaatctat taataacgca tgtttatgcc gatctatagc catactattt aagtcatgaa
    10261 gataagtttg ataactgggc gattccgtta gttgagcaac tattaaatca tctagccttt
    10321 gctcacgctt tgacacgttt gcgaagtgaa tttgaatatc aaaagcacag ttatgattag
    10381 cgatataatc aaatatttca tttgtattca ttaactttat attacgctta gtaaattgaa
    10441 ttgcagaagc gtgacttccc acttctgcaa tttctaatgt ttcatgatga ttaatttttg
    10501 tatctacaaa atgaatgttt gccaatttcg cctcattcac ttttatatag ttaagcaccc
    10561 aaactgcaat acgcgactta aatcgatatt gaaaaagtaa atattcaata aaactttctt
    10621 taatttgatt gaatgtctct gacatcaaat accccatttt aagattgcaa tcttgataat
    10681 tcgtcatgcc aattttcgtt acttggctct agttccaaca attgatttaa aatagtaatt
    10741 gcttgttcct tttgaccaat ttcaattaaa tagaaataat aatcactcat aaaatcaata
    10801 tttgttttca tcgttggata tgctaattca aagaaatgtt gagcttcttt atctcgctct
    10861 tcttgaccat aggcgaacgc taaatgccac atgaatgtag gatccaaatc ttcttcatct
    10921 acatatgtta ataattcaat gattgcttca taatcttctt cattacgata taaatcgctt
    10981 aaaatcaata aaggttcttg gtaagcatta tcaacctcta atgcttgttt taacaataat
    11041 acaccttcat tagcatcgcc gtgttctatt tccaaacatc cagttgtata cattaactct
    11101 ttataaaatt gacttagtcg taatccttct ttacccgttt caatggcatc tggataattt
    11161 ttttcatttt catataatga ttgtaaatac aagtagcctt gaatataatc aggatcttta
    11221 gaaagtaatg tagtcattat tttaattgct tcttgagtga tgtcattttt atcgtaagaa
    11281 atggcttttt tgagataatc ttctgaagtc atttcatctt cattaatttc atcgtataag
    11341 cgaatcgcat cactatagtt accactttgt aaactacaat ctgccatacg agagaataag
    11401 tttacaccat taacttgata ttcaccagtt tctaaaacgg tttcgtattc agaggtagca
    11461 cgtaaatatt gaccatcata atataacatt tcagccaatg caaaatggat tattggatca
    11521 tttggctcta gttcaagtgc ttcttgtaat ttatcaatag caacttccat catattaatt
    11581 tgttgatata aatctgcttc taacatcaac ttttcaggtg atggttcaac ataacttaaa
    11641 tattctaacg cttcgtcagt ttgattttca gacattaaac cttcaataaa ataaatcagc
    11701 aattcacttt cgtctggata tttgtgatat aacacgcgga atacttccaa accttgtggc
    11761 attaatccaa aattgtaaag tgtctctcct agaataaata atgcgtcatc gttgtcagta
    11821 gttattgctt cattaacacg agagtctaaa ttttctagtt tttgtagatt gatatcgtct
    11881 attaatttat agatatcttc cattttatat tatccctcat tttctaaaag ctttagtttt
    11941 ggtaaaaatc ctggaaatga tacatttaca gcatcaaatt gtttgatttt gacaggctcg
    12001 cttgaaagta gagaagcaac tgcaagcatc attcctattc gatgatcagt taaactatca
    12061 actgttgcat ttgttttaaa ttctgacgga tgaataatca atccatcatt agttggttgt
    12121 aattcaaacc ctaacaagtt taacatatca gccgttgtat caattctatt tgtttctttt
    12181 acttttaatt cctcggcatc tttaattgta ctcgtgccaa ctgcttgtgt acaaagtaat
    12241 gctattacag gcagttcatc aattgctttt ggaactaatt ctccttcgat tgttattggt
    12301 tgaagcattg gtgtgtattg aatacgaata gaagcagtag gttcagcacc agttgtttga
    12361 ttgaaaagtt ggatattacc gcccattttt tcaacaatat caataatacc tgaacgtgtt
    12421 ggattgattc caacattatg aattgttaca tcacttcctg gtgtgataag tgctgcaaca
    12481 ataaagaacg ctgcagatga aatatcgcca ggaacatgaa aatctgcagg tttaatgtat
    12541 cgaattgctt caggggttgt attaattgat aacccttctg cttcaattgg aatattaaaa
    12601 tgtttgaaca tcgtctcagt atgatttcga cttacatcta attctttaat gatggtcggt
    12661 tccttagaaa acaaacttgc aaataaaatg gcacttttta cttgtgcact tgcaacttcc
    12721 atttgataat ttataccttt tatgacagat ggcttaataa ttaatggtgt ataattatct
    12781 tcaatacctt caatattcgc atccataagt ttcaatggtc tcaagacacg atccattggc
    12841 cttttaccaa ttgaaacatc gccagacaaa acactttcaa tacctaaacc acttaacaaa
    12901 cctgccaata atcgtgttgt cgtaccagaa ttacctgtat acaatacttg atgtggcgtg
    12961 ttaaaagatt gatatcctgg ggaagtcaca actaattttt catcatcttc tttgatttct
    13021 acacctaaca gtcggaaaat gtccatcgta cgacgacaat cttcgccaag tagtggctta
    13081 tatatagtag atacaccttc agctagcgac gccaacatga ttgcacggtg tgtcattgac
    13141 ttatcgcccg gcacttctat ttcgcccttt aacggacctg aaatatcaat gatttgttca
    13201 tttaccattt cattcaccta cttaaaatat gtttttaatt gttcacatgc atgttgtaat
    13261 gttagttgat caacatgttg tacaacgata tctccaaatt gtctaatcaa gaccatttgt
    13321 acaccttgct tatcattctt tttatcactt agcatatatt ggtataacgt ttcaaaatcc
    13381 aagtcagtta tcatgtctaa aggatagccg agttgtatta aatattgaat ataatgatta
    13441 atatcatgct tagaatcaaa caaagcattc gcaactataa attgatagat aatgccaacc
    13501 atcactgcat gaccatgagg tattttatga tagtattcaa cagcatgacc aaatgtatga
    13561 cctaaattta aaaatttacg tacaccttgt tctttttcat ctgcaataac aatatccagc
    13621 ttcgtttcaa tacctttagc aatatattta tccataccat ttaatgactg taatatctct
    13681 ctatctttaa agtgctgttc gatatcttgc gtcgctgatt caccattcaa taacgcatgc
    13741 ttataaactt ctgcatagcc acttaatatt tgctcaaatg gtaacgtctt taaaaagtct
    13801 aaatcataaa tcacagcagt tggacgataa aatgcaccga taaggttttt accttgcttt
    13861 gagttaatac ccactttacc gccaacacta gaatcatgcg ctagtatagt cgttggcact
    13921 tgtataaagt gaacgcctcg taaaagtgtc gccgcaataa acccagcaaa atcaccagtt
    13981 gcaccaccac caacagcaat aattgctgta ttacgagtta catgatggga taaaatatac
    14041 tctaatgttt cttgatattg ctcaaatgtt ttcgtctttt caccagctgg aataataact
    14101 ttatgtacat tttcatatga taaaatatca tcaaatttat cagcaaaata ttgatttaca
    14161 tgctcgtcaa ttaatataaa actttgatca aactgatcaa tatacgtgct aatatggtca
    14221 attgcaccgt gttcaacata tattggataa ttatttgaag ggtatgttgt ttgtaatttc
    14281 atgattacac ctcaattgtt cttgttgtta aaactcaata tttaattgtc tgcgctcaat
    14341 aatttgttgt ttaagttgct caatatgatt tgattggaat tcttccaata atgcttttgc
    14401 tatttcaaat gctacgacat gttcgcagac gatacttgct gcaggaacag cacaactatc
    14461 agaacgttca attgttgctt taaagtcttc tttagtatta atgtctactg aatttaatgg
    14521 tttatataac gttggaattg gtttcattac accattaacg ataattggca ttccatttga
    14581 cataccgcct tctaaaccac ctaagtgatt agatccacga taataaccaa tttcactatt
    14641 atatagaatt tcatcttgaa tctcactacc tggcttttca gctgctttaa atccttcacc
    14701 aaagcttaca cctttaaaag catttatgct gacaacacct tgtgcaatct taccatctaa
    14761 cttacgatca taatgcacat aactacctac accaacaggc atattttcaa ctacaacttg
    14821 aacgacaccg ccaattgaat ctccttcatt tttagcttcg tcaattttat ctcgcattgc
    14881 ttgtgcgata ctgtcatcaa ttacacgaac atcattacga tcaagatttg ctttaaatgt
    14941 ttctgaatca taaaaatctt tatctttaat tccacctatt tcaacaacac gactgtatat
    15001 atcgatatct aactgttgta ataacacttt acataaggca ccgactgcaa ctcgagctgc
    15061 tgtttctcta gcagatgatc gctctagcac atttcgtaaa tcacgatgat tatatttcat
    15121 acctccaacc aaatctgcat gaccaggtct tggttttgta atagtacgtt tcatattttc
    15181 acgttcttct tcacttattg gagctgctcc cataattttt ctccaatgcg taaagtcatc
    15241 attggttaca accatagtaa ttggactacc taatgtataa ccatttctaa cgcctgatac
    15301 tatttctact gtatctttct caatttgcat acgacgtcca cgtccgtaac cgccttgacg
    15361 cttaaacatt tctttattaa tatcctcaac cttaatttct atatttgcag gtacaccttc
    15421 aacaataact gttaattgag gtccatgtga ttctcctgat gttaggtatc tcatacacac
    15481 tcgctccttt catacttcca cgataacatt ataatatgtt gttaatctta tcatattcat
    15541 tgtgcattca taaatgtttg ttttcaagct caattaagaa aatggatgac atctcatttt
    15601 aaatgtcaga attatcttaa tattaaacga actaaaatta attataatat attcgtctgt
    15661 agaaaagtat catgaatttc taaacagttg catcatataa tcattaatta aaaatgatta
    15721 gccgttttaa tataatcgta tcgcaacttt agcaaataat atactgacta catttcttaa
    15781 tacaaaagaa cgaactgtta attattaata aagttaatca gtccgt
//
LOCUS       DALAGF010000068        15759 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.068, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000068 DALAGF010000000
VERSION     DALAGF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15759
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.068"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            99..1445
                     /locus_tag="O2692_001857"
     CDS             99..1445
                     /locus_tag="O2692_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCZ4299482.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFYLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            1455..2345
                     /locus_tag="O2692_001858"
     CDS             1455..2345
                     /locus_tag="O2692_001858"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCZ4299483.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            2471..3256
                     /locus_tag="O2692_001859"
     CDS             2471..3256
                     /locus_tag="O2692_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCZ4299484.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            3298..4161
                     /locus_tag="O2692_001860"
     CDS             3298..4161
                     /locus_tag="O2692_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCZ4299485.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            4148..4558
                     /locus_tag="O2692_001861"
     CDS             4148..4558
                     /locus_tag="O2692_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCZ4299486.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            4835..5434
                     /locus_tag="O2692_001862"
     CDS             4835..5434
                     /locus_tag="O2692_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCZ4299487.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            5555..7630
                     /locus_tag="O2692_001863"
     CDS             5555..7630
                     /locus_tag="O2692_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCZ4299488.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIIFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVTINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     NDKNKDKDK"
     gene            7743..7892
                     /gene="rpmG"
                     /locus_tag="O2692_001864"
     CDS             7743..7892
                     /gene="rpmG"
                     /locus_tag="O2692_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCZ4299489.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            8101..8640
                     /locus_tag="O2692_001865"
     CDS             8101..8640
                     /locus_tag="O2692_001865"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCZ4299490.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQAA"
     gene            8652..10115
                     /locus_tag="O2692_001866"
     CDS             8652..10115
                     /locus_tag="O2692_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCZ4299491.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDNIYNKLIKDDMTSGNYDNAQNIVKQ
                     TINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFELAIANRSLND
                     DEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK"
     gene            10096..10299
                     /locus_tag="O2692_001867"
     CDS             10096..10299
                     /locus_tag="O2692_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCZ4299492.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            10296..11282
                     /locus_tag="O2692_001868"
     CDS             10296..11282
                     /locus_tag="O2692_001868"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCZ4299493.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            11282..11611
                     /locus_tag="O2692_001869"
     CDS             11282..11611
                     /locus_tag="O2692_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HCZ4299494.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            11608..12231
                     /locus_tag="O2692_001870"
     CDS             11608..12231
                     /locus_tag="O2692_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCZ4299495.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            12283..13257
                     /locus_tag="O2692_001871"
     CDS             12283..13257
                     /locus_tag="O2692_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HCZ4299496.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMHKYI"
     gene            13229..14299
                     /locus_tag="O2692_001872"
     CDS             13229..14299
                     /locus_tag="O2692_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCZ4299497.1"
                     /translation="MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     LFIVAIYLVIMLPMFQMMQSIK"
     gene            14313..14624
                     /locus_tag="O2692_001873"
     CDS             14313..14624
                     /locus_tag="O2692_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCZ4299498.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            14602..15048
                     /locus_tag="O2692_001874"
     CDS             14602..15048
                     /locus_tag="O2692_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCZ4299499.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYENSDTIKVIENNNIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIYNNNSIYRIIFHIEKGRIRYEKL"
     gene            15035..15334
                     /locus_tag="O2692_001875"
     CDS             15035..15334
                     /locus_tag="O2692_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HCZ4299500.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            15252..15749
                     /locus_tag="O2692_001876"
     CDS             15252..15749
                     /locus_tag="O2692_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCZ4299501.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDILDDFKGVDRNDIEIRQHRIILH
                     KGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
                     KTIYV"
BASE COUNT         5889 a         2045 c         2799 g         5026 t
ORIGIN      
        1 tttctaaatt ttgaaatttc gaggttttaa tatttttatt taaaatagca catttatact
       61 ttataatagt aaagatgaac atataaggag gccaaatcat ggcaaaacat ccattcgaac
      121 aatttaatct agaatctagt ttaattgacg ctgtgaaaga ccttaatttt gaaaaaccaa
      181 ctgaaattca gaatcgaatt attccaagaa tactaaagag aacaaattta attggtcaat
      241 ctcaaacggg tacagggaaa tctcatgcat ttttattacc attaatgcag ttaattgata
      301 gtgaaataaa agaaccacaa gcaatcgtag ttgcaccaac aagagaactt gcacaacaac
      361 tatacgatgc agcgaaccat ttaagccaat ttaaagctgg tgtttcagtt aaagttttta
      421 ttggtggtac agatatagag aaagatagac aacgttgtaa tgcacaacca caattgatta
      481 taggcacccc tactagaatt aatgacttag ctaaaacggg acatttacat gtgcacttag
      541 catcatattt agttattgat gaagcggatc ttatgattga cttaggatta attgaagatg
      601 tagattacat tgctgcaaga ttggaagata atgcaaatat tgcggtgttt agtgctacaa
      661 tcccacaaca gttacaacca tttttaaata aatatttaag tcatccagaa tatgtagctg
      721 tcgacagtaa aaaacaaaat aaaaagaaca tcgaattcta tttaatacct actaaaggtg
      781 cagctaaagt tgaaaagact ttaaatttaa ttgatatact aaatccatac ttatgtatta
      841 ttttctgtaa tagtagagat aatgcaaatg atttagcacg ttcactaaat gaagctggta
      901 ttaaagttgg tatgattcat ggtggcttaa cgccacgtga acgtaaacaa caaatgaaac
      961 gtatacgtaa tttagaattc caatacgtta ttgccagcga tttagcatct cgtggtattg
     1021 atattgaagg tgttagtcat gtcatcaatt ttgatgtgcc aaatgatatt gacttcttta
     1081 cgcatagagt cggacgaact ggtcgtggga attataaagg tgtagcaatt acgctttata
     1141 gtcctgatga agaacacaat atttcattaa tagaagatcg cggttttgta ttcaatactg
     1201 ttgatattaa agatggtgag ttaaaagaag ttaaagcgca caatcagcgt caagcaagaa
     1261 tgcgcaaaga tgaccattta actaatcaag tgaagaacaa agttcgaagt aaaattaaaa
     1321 acaaagttaa accaggttat aagaagaaat ttaaacaaga agttgaaaaa atgaaacgtc
     1381 aagagcgtaa gcaatttagt aagcagcaaa atagacaaaa acgtaagcaa aacaaaaaag
     1441 gttaggtgtg ttttatgtta ttaggatcac atgtttcaat gagtggtaaa aagatgttag
     1501 aaggttctgc tatagaagcg catgaatatg gtgaaacaac atttatgatt tatactggtg
     1561 cacctcaaaa cacgcgccgt aaaagtattg aagatttaaa tataactaaa ggtcatgaag
     1621 tgatggaaaa atatggttta tctaatattg ttgttcatgc accatacatc attaatattg
     1681 caaatacaac caaaccagaa acgtttaatc ttggtgtaga tttcttgcaa caagaaattg
     1741 aaagaacgca agctataggg gctaaagata ttgtattaca tccaggtgcg catgttggag
     1801 ctggtgttga tgccggaatt aataagatta ttgaaggatt aaatgaagtt ttaacgaatg
     1861 acaataatgt acgtattgcg cttgaaacga tggcaggtaa aggtacagag attggtcgct
     1921 catttgaaga gttagcacgt attattgatg gagttcataa taacgaacgt ttatcagtat
     1981 gttttgatac atgtcatacg catgatgctg gatataatgt taaagaggac tttgatggcg
     2041 tattaaatga atttgataaa atcattggag tcgacagaat caaagttgtt catgtcaatg
     2101 attctaaaaa tgaccgtggc gctcaaaaag accgacatga aaatattggc ttcggttaca
     2161 ttggttttga tgcattaaat tacattgtgc atcatgatag ttttaaagat attccgaaaa
     2221 tcttagaaac accttatgtt ggtgaagata agaaaaataa aaaaccgcca tataaacttg
     2281 aaattgaaat gttaaaacaa cagcaatttg acccagaatt aaaaaataag gttatgcaac
     2341 aataaaatga taatgctctc ttcaagaaat gcgataaata aaacttgaag ggggcatatc
     2401 tctttatttt gtctaatttt gagtcgtaaa cattactgtt tacatatgtt actaggagtt
     2461 gtatagttga atgcgaggtg agaatacgat gacaccagtc tttgaattga aaaatgtcaa
     2521 ttactactat gatcataaaa aagtgttaga aaatataaac attaaaataa ataaaggtga
     2581 atttttagca attgttggac caaatggtgc tggtaaatca acattattga agttgattct
     2641 agggttatta cctttacaaa gtggtgagat ttttgttgaa ggtattgatt ttaaaaataa
     2701 gaaaacatca attaaattaa gctatgtatc acaaaaagca aatgccttta attcaggttt
     2761 cccagcaagt gttaaagaag ttgttttaag cggattaaca aagacaaaac gtcttttcca
     2821 aacatttaat agcaaagata atgaaaaagt gattaaagta ctagaaagac tgaatataag
     2881 tgatttaatt cataaaaata tagcagaatt atcaggtggt caacaacaac gtgtaatgat
     2941 tgctcgagca ttgatttcag aacctgcagt attagtactt gatgaaccaa cgaatggtat
     3001 tgatgcaaaa catgtaagtg aattttataa tactttagat caattaaaac aagaaggtat
     3061 caccattatc ttagttactc atgatatcgg tgttgtagca gatactgcta ctgaagtagc
     3121 atgtttaaat aagcatttgc atttccatgg tacaactgat gagtttaaat cacttgatga
     3181 agttgaaatt tcaaaaattt atggacatcc tgtacgtttt gtcgatcatc agcataatcg
     3241 agaatgttgt aattaataaa gatttgagtt tatttaaaat ggaaaggcgt gtcacaattg
     3301 atagatgcat tattgaattt tgattttatg aggtactctt taattagtgg tatcttgata
     3361 ggttttattg cgcctctaat cggtgctttt atcgttgtta gacgactatc acttatagct
     3421 gatgctctaa gtcatgtaac tttaggtggt atatctttcg gtatgttttt acttactatt
     3481 atgccaacac tagtatttat taatccaatg tggtttggaa tcttattcgc aatagtaggt
     3541 gcgcttctaa ttgaaaaatt aagaacgtca tacactgctt accaagaaat tgctattcca
     3601 attataatga gtgctggtat cgccttgagt gcaatcttca tttcattagc tgatgggttc
     3661 aatcaagaaa ttgttggatt gctatttggc tcaataagcg cagtgaatat aagtgattta
     3721 acaacgatta ttgtcattgc catcatagtt gttctgttta ttacattgtt ttataaagaa
     3781 ttatttatat tgtcatttga tgaggaatat agtaaagtca ttggcattcc taaatggatt
     3841 caatttttat ttattgttat tgtagcgatg gttatttctg cctcaatgcg tgttgtaggg
     3901 atactgttag taagtgcgtt aatcacgtta ccaattgcaa taagtatgcg aataacaaaa
     3961 agttttaagc aattgatatt actaagtgta tttttaggtg aattatcagt aatattaggc
     4021 ttagtcctag ctttctatat ggacatttct ccgggtggtg taattgtagt tctacttgtt
     4081 attttactta tgattacaat ggcttatcag aaaatgcgaa tgaagtttaa aaagggagct
     4141 aatatcaatg aatacaaatg atgctattaa aattttaaaa gagaacggtt taaaatatac
     4201 agataaacgt aaagatatgt tagatatttt tgtcgaagaa gataagtata taaacgcaaa
     4261 gtatatacaa caagttatgg atgaaaatta tcctggaatt tcattcgaca caatatatag
     4321 aaacctgcac ttatttaaag atttaggaat tattgaaaat acagaacttg atggtgaaat
     4381 gaagtttaga atcgcttgta caaaccatca tcatcatcat tttatctgtg aaaagtgtgg
     4441 agatacaaag gtaatagatt attgtccaat agatcagata aaattatcac tacctggtgt
     4501 taatattcac aaacacaaac ttgaagttta tggtgtatgt gagtcttgcc aagattaata
     4561 taaagaaatg agatttatgc acatttggtc agatgtatgc ataaatctca tttctttata
     4621 ttatagaaga taaaggttca tttcatactc cgttcgtatg attttatggc aaatctgtta
     4681 actttttata tacaaaatgt ttaattattt ttgatttgag ttatattaaa tgagtagaga
     4741 aaataataag tagtagaatt taagcaaatt ctttgttgaa ccagtcactg cttgttatta
     4801 tttacttaca gacattttag gaggatgatt atttatggct tttgaattac caaaattacc
     4861 atacgcattt gatgcattag aaccacattt tgacaaagaa actatggaaa ttcaccatga
     4921 cagacatcat aacacgtatg ttacgaaatt aaatgctgca gtagaaggta cagatttaga
     4981 atctaaatct attgaagaaa ttgttgctaa tttagacagt gtaccagcta acatccaaac
     5041 tgctgtacgt aataatggcg gtggacattt aaaccattca ttattctggg agttactttc
     5101 accaaactca gaagaaaaag gtactgtagt agaaaaaatt aaagaacaat ggggttcttt
     5161 agaagaattt aaaaaagaat ttgctgacaa agcagctgca cgctttggtt caggttgggc
     5221 ttggttagtc gtaaacaatg gccagttaga aattgtgact acaccaaacc aagataatcc
     5281 attaactgag ggtaaaacac ctattttagg tttagacgta tgggaacacg cttattacct
     5341 aaaatatcaa aacaaacgcc ctgactacat tggcgcattt tggaatgtag ttaactggga
     5401 aaaagttgac gaattatata atgcaacaaa ataaagtatg attacttgtt cggtctctta
     5461 aatgagaccg ttattttttt gtcaaaaaga tagaaataat ttctaaattc atatatgatt
     5521 taaagtgaaa gactttgaat agaggtaggt agttttgtta aaaagactaa aagaaaaatc
     5581 aaatgatgaa atcgttcaaa atacaattaa caagagaatt aactttatat ttggtgtgat
     5641 tatatttatt tttgcagtac tagtactacg tttaggttat ttacaaatcg cacaaggctc
     5701 acattataaa caaattataa aaaatgatga aaacattaca gtgaatgagt ctgtgccaag
     5761 aggtcgtatt ttagacagaa atgggaaagt tttagttgat aatgcttcta aaatggctat
     5821 tacatatact aggggtcgaa aaacaacaca atcggaaatg ttggatacgg ctgaaaagtt
     5881 atcaaagcta atcaagatgg atactaagaa aattacagaa cgtgataaga aagatttctg
     5941 gattcagttg catcctaaaa aagcaaaagc aatgatgaca aaagaacaag ctatgttagc
     6001 agatggaagt attaaacaag atcaatatga taaacaactg ttatcgaaaa tcggaaaatc
     6061 acaattagat gaattgtctt ctaaagattt acaagtttta gctatttttc gagagatgaa
     6121 tgcaggaaca gttttagatc cacaaatgat aaaaaatgaa gatgtcagtg aaaaagagta
     6181 tgcagcagtt tctcagcaac tttccaaatt accaggtgtt aacacgtcta tggattggga
     6241 tagaaaatat ccatatggcg atactttaag aggtatattc ggagatgtat cgacacctgc
     6301 tgaaggtatt ccaaaagaat tgacagaaca ttacttatcc aaaggatatt cacgcaatga
     6361 tcgtgttgga aaatcttacc tagaatatca atatgaagat gtattgcgtg gtaagaagaa
     6421 agaaatgaaa tacacaacgg acaaatctgg taaagttaca tcttcagaag tgttaaatcc
     6481 tggcgctcgc ggtcaagatt tgaaattaac gatcgatata gatcttcaaa aagaagtaga
     6541 agcattatta gataaacaaa ttaagaagct tcgcagtcaa ggtgccaaag atatggataa
     6601 tgcaatgatg gttgtacaaa atcctaaaaa tggagacatt cttgcgcttg ccggaaagca
     6661 gattaataag agtggtaaaa tgactgatta tgacattggt acgtttactt ctcaatttgc
     6721 ggttggatct tctgtaaaag gtggaacatt attagccggt tatcagaata aagctatcaa
     6781 agttggagaa acaatggtcg atgaaccatt acatttccaa ggtggtttga caaaacgatc
     6841 atacttcaat aaaaacgggc atgtaactat taatgataag caagctttga tgcattcatc
     6901 aaacgtatat atgtttaaaa cagcattaaa attagcggga gacccttatt attctggtat
     6961 ggctttacct tcagacataa gttcacctgc ccaaaagcta agaagaggat taaatcaagt
     7021 aggcttaggt gtgaaaacag ggatagattt accaaatgaa acaagaggtc aaatcgaacc
     7081 attaacaaat aatccaggta attatctaga tttatcaatt ggtcaatatg atacctatac
     7141 accattacaa ttatcacaat atgtttcaac tatagcgaat gatggttata gaatacagcc
     7201 acacattgga ttaacgattc atgaatcaac taataaagat gaggttggtc cactcaagaa
     7261 gaaaattaat ggcactgtct tgaacaaggt taataatact gaaaaggaaa tcaaacaaat
     7321 tcaagaagga ttcaaaatgg catttaatga taaagatggt actggatatg ttagttttaa
     7381 agatacagta gtacctactg ctggtaaaac gggtaccgca gaagtgttcc aaaacggaga
     7441 gccaagagtt aactctactt atataggata cgcgccaatt gatgatccaa aattagcgtt
     7501 ttcaattgta tatacaaatc agcctgtacc accaccatgg ttaacaggtg gagacttagg
     7561 tagagatgta attaactact actttaagca gttaggtaaa aatgataaaa ataaagacaa
     7621 agacaaataa aatttaacct gacgattgtg tagcgcatgg ttgtaaaatt ttaactttgc
     7681 aaatattata gatgttggta taataataaa gtcgtattta gaaatgataa ggaggaataa
     7741 ccatgcgcgt aaacgtaact ttagcttgta cggaatgtgg tgacagaaac tacattacaa
     7801 ctaagaacaa aagaaataat ccagaacgtg ttgaaatgaa gaaattctgt tcacgtgaaa
     7861 acaaacaaac tttacaccgt gaaacaaaat aatcattaaa attaaattta aattaacaat
     7921 aaaaaaggtg aaatctcaaa ataagaggtt tcaccttttt aattatgcac acgtatgaaa
     7981 tgataaaaga ttttcaattg aaataataaa ttttagaatt gtcgcataat tcatcatgac
     8041 aacataatga catgtattgt tattaacgat ataatagaat tgaataaagt gggtgataat
     8101 gtgactaaaa atgagattag aaaatacatt ttacataaaa tgaagaattt taataaagct
     8161 gaaaagcgaa aagcagacac atggttaaga aatcaatttt ttgcaactga agaatacaaa
     8221 gaagcaaacg caattgcgct agttctttct tttaatcatg aagtagatac tttttctatt
     8281 attgaacaag ccttaatgga tcataaacgt atttttgtac cgaaaatgga ttatttaaat
     8341 catcaaatga cttttaaaga gatatttaat ctcaaagata ttgatgtcga taataagggg
     8401 atttactatc caacttcaaa aggtgaaaca acgaataacc tagatttaat tgttgttcct
     8461 ggtgttggat ttcaagacga tggatataga attgggtatg gtggtggcta ttacgacagg
     8521 tttttagcta attatcagac aaagacaata agcttattat acgattttca aataacatca
     8581 tttgaaccgg aatcattcga tcaaccagtc gataaattga ttatatatca agcagcatag
     8641 tggaggacta aatgaacata gacaaacaat tttggaaaac aatatattat tggattaggt
     8701 acttgaattt tgatattgtc agtagggaaa aagatgacca agaaatttgg ttagcccata
     8761 aaagaaaaaa acaagtagtc atttttaaac aacatatcaa gtctacccaa gaaatacgtt
     8821 ttgacaaagc gaaagtgctt gaacacaaag atgaaatagc aaattttatt tctttcgaac
     8881 cacaaagttt tgaattttat tattttacag aatcggaatt ttcagaagaa caattaaatg
     8941 aagtttcgcc aattagaatt aaattcaatg ttataagaca cacaaaagat ttgataaagc
     9001 atatgccgaa tatatttttg gctagactta tttcagaaga taatgataaa aagacatata
     9061 tgttctataa acgcaaagta ttaaccgata actttttaga taaatatatg cagaaatttt
     9121 caccggcaac atacacaata atatttgtaa atgtcttaat atggttatgt atgattttat
     9181 atttaaataa tttttcggat gtaaaattat tagatgttgg cgggttagtg cattttaatg
     9241 tcgtacatgg tgaatggtat cgaattgtta catcgatgtt tttacatttt agttttgaac
     9301 atatacttat gaatatgctt tcattattta tttttggtaa aatagtcgaa gcaattattg
     9361 gttcatggcg gatgttaact gtatacttta ttgcagggtt gtttggaaac tttgtatcac
     9421 tatcatttaa tacgactaca atttcagttg gggctagtgg tgctatattt ggtctgattg
     9481 gatcaatttt tgcgatgatg tatgtttcaa aaacatttaa caaaaaaatg ttaggacagt
     9541 tattaattgc attagtgata ttagttggtg tttctctgtt tatgtcaaat ataaatattg
     9601 tggcgcatat tggaggattc attggtggtt tattaataac tttaattggc tattactata
     9661 aagtgaatcg taatattttt tggattttac taattggtat gcttgttata tttattgcac
     9721 ttcaaattag aatttttaca attaaagaag ataatattta taataaattg atcaaagatg
     9781 atatgactag tggtaattat gataatgctc aaaatattgt gaagcagaca ataaataaaa
     9841 attatgccga tgatcaaaca tattatttaa gtggtatgat tatggctact atcaattcta
     9901 agtccgaagg tatgacagaa tgggagcgag gacttagaat gtttcctaaa tcaggtttat
     9961 taaattttga gttagcgata gcaaatcgtt cattaaatga tgatgaaaaa gcattaaaat
    10021 atgtgcgtaa agcattaaat gcagacccta aaaatacaga ttatattaac ttagaaaaag
    10081 agttgactaa atcaaatgag tcgaaaaata aataactttt atgatgtaca acagttattg
    10141 aaaagttacg gatttctaat atattttaaa aatccagaag atatgtacga aatgattcaa
    10201 caggagattt catcattgta tcaatatgaa ctgttatcta aggaagaata tttgaaatgt
    10261 acgttgataa ttaatcagag aaggaatgaa cagaaatgac aaaaattatt ttagcagctg
    10321 atgtaggcgg gacgacttgt aaattaggta ttttcacacc tgaattagaa caattacata
    10381 aatggtctat tcacactgat acatctgata gtacaggata tacacttttg aaaggaattt
    10441 atgattcgtt tgttgaaaaa gtaaatgaaa ataattataa tttttcaaat gtacttggcg
    10501 taggtattgg tgtaccaggt cctgttgact ttgaaaaagg tacagtaaat ggagcagtaa
    10561 acttatattg gccagaaaaa gttaatgtac gtgagatttt tgaacaattc gttgattgtc
    10621 cagtgtatgt agataatgat gctaacatag ctgctttagg ggagaaacac aaaggtgctg
    10681 gtgaaggtgc cgatgatgtt gttgccatca cacttggtac aggtctaggt ggaggaatta
    10741 tttcaaatgg tgaaatcgta catggtcata atggctctgg cgcagaaata ggtcatttta
    10801 gagcagactt cgatcaacga tttaaatgta attgtggtcg ttctggatgt attgaaacag
    10861 ttgcttcagc gacaggcgtt gttaacttag ttaacttcta ctatccgaag ttgacgttta
    10921 gatcttctat attagaattg attaaagaaa ataaggttac agcaaaagct gtttttgatg
    10981 cggcaaaagc tggtgaccaa ttctgtattt tcattactga aaaggttgca aactatattg
    11041 gatatttatg tagtattatt agtgttacaa gtaatccgaa atatatcgtt ctaggtggag
    11101 gaatgtctac tgcaggacct attttaattg aaaatattaa aacagaatat cataatttaa
    11161 catttgcacc tgctcaattt gaaactgaaa ttgtacaagc gaaattaggt aatgatgcag
    11221 gtattacagg agcagcagga ttaatcaaga cctatgtatt agataaagag ggggtaaaat
    11281 aatggctatt gttgatgtgg ttgttattcc agttggaacg gaaggtccga gtgttagtaa
    11341 atatattgca gatattcaga aaaaacttca agaatataaa gcaatgggta aaattgattt
    11401 tcaattaaca ccaatgaata ctctaattga aggtgaatta agcgatgtat tagaagttgt
    11461 gcaagtgata catgaattac cttttgataa aggtttaagt agagtttgta caaatatccg
    11521 tattgatgac cgacgagaca aatctagaaa aatgaatgat aaactaacat cagtacaaaa
    11581 acatttagaa aatagtggtg aaaacctatg aggatttcaa gcttaacttt aggcttagtt
    11641 gatactaata cgtatttcat cgaaaatgac aaagctgtta ttctgattga cccttcaggt
    11701 gaaagtgaaa aaattattaa aaaattaaac caaataaata aaccgttaaa agctatttta
    11761 ttaacacatg cacactttga tcatatcgga gcagtcgatg atatagttga tcgattcgat
    11821 gtcccggttt atatgcatga agcagagttt gattttctaa aagatcccgt taaaaatggg
    11881 gcagataaat ttaagcaata tggattacca attattacaa gtaaggtaac tcctgaaaag
    11941 ttaaacgaag gtagcacaga aatagaagga tttaagttta atgtgttaca cacacctgga
    12001 cattcaccag gaagtttaac atatgtgttc gatgaattcg cagttgttgg agatacatta
    12061 tttaataatg gaatcggacg tacagattta tataaaggtg attatgaaac gctagttgat
    12121 tctattcaag ataaaatatt tgaattagaa ggcgatttac ctttattccc tggacatggt
    12181 ccatatacga cggttgatga tgaacaatta aatccatttt tacacggtta aattgaagta
    12241 ataaaaacac ctcctacgtg attatatgta ggaggtgttt ttttgaagat tctatttcaa
    12301 gaaataatta ataaagcgat agaaatgaaa gcgagtgatg tacattttat tccagttaaa
    12361 aatgaagtaa gtattaaatt tagaattaat gataacttgg agcagtatga acaaattggg
    12421 aatagcattt atcaaaagtt attagtttat atgaagtttc aagctgggct tgatgtttct
    12481 acacagcaag tcgcacagag cggtcgatat agttaccttt tcaataaaat atattttttg
    12541 agaatatcaa ctttaccatt gtcacttggc caagaaagtt gtgttatcag aattgtacct
    12601 caattttttc aaccacagaa atcaacttat aaattcaatg attttaaaca cctcatgaat
    12661 aagaaacaag gattactatt gtttagtgga ccaactggtt caggaaagag tacattaatg
    12721 tatcaaatgg tctcatacgc gaataaagcc ttgaatttaa atgtaatttc tatagaggat
    12781 cctgtagaga tgcaaattcc tggtatcgtc caaattaatg tgaatgataa agctggcata
    12841 aactatgtaa attcgtttaa agctatttta agatgtgatc ctgatgttat tttaataggt
    12901 gaaatcagag ataaagatgt tgccaagtgt gttatacagg ctagtttaag tggtcacctt
    12961 gttctgacta cattgcatgc aactgattgt aaaggtgcta ttttaaggct attagaaatg
    13021 ggcatttctg tacaagaatt gatacaggca actaacttaa ttataaacca acgacttgta
    13081 actactatta agcaacagcg acaattagta tgtgaaattc tatctcagca acaactccga
    13141 tatttctttt cccataatca ttcattacca tcatcattta agaacttaga agataaactt
    13201 gatgatatga caaaagcagg tgtcatttgt gaaactacaa tgcataaata catttaaact
    13261 acattctaag aagcgacaat taagtaaggc ccaacaaatc gacttacttt caaatttatg
    13321 taatttgttg aaatatggtt tcactctgta tcaaagtttt caatttttaa atcttcaaat
    13381 gacatataaa aataagcaat taggtaccac cattctaagt gaaatttcaa atggtgcacc
    13441 atgcaatcaa atattatcac tgataggtta tagcgatact atcgtcatgc aagtatattt
    13501 ggcagaaaga tttggcaata ttatagacgt tctagaagaa accgtaaatt atatgaaagt
    13561 gaatagaaag tcagaacaac gattgttaaa gacactgcaa taccccttaa tactagtttc
    13621 tatctttatt gctatgatta ttatattaaa cctcacagta attccacagt ttcaacaatt
    13681 atatacttct atgaatattc aactatcttc ttttcaaaaa acattgtctt ttttcattac
    13741 cagcttacct actataattg tagtaatgct cataatagta tctatgttgg ctattattat
    13801 gaaattaatt tataacaatt taaatatgct caataagata aactttgtga tgaaactacc
    13861 gctaatatca ggctatttcc aattatttaa aacttatttt gtaactaatg aattagtgtt
    13921 gttttataaa aatggtatta cacttcaatc aatagtagac gtttatatta accatagtag
    13981 tgatccattt agacagtttc taggtaaata cttattaact tattcagaaa tgggatatgg
    14041 tttgcctcaa attttagaaa aactaaaatg ctttaagcct caattaatta agtttgtgct
    14101 acaaggtgaa aagagaggga agctagaagt agaactaaag ttatattcgc aaatattagt
    14161 aaaacaaata gaagataaag cgataaaaca gactcagttt ttacagccta ttttattttt
    14221 gattttaggt ttatttattg tcgcaattta tttagtaatt atgttaccaa tgtttcaaat
    14281 gatgcaaagt ataaaataat ggaggttaat atatgtttaa atttcttaag aaaactcaag
    14341 cgtttacatt gatagagatg ctattagtgt tattaatcat cagtttatta ttaattttaa
    14401 tcattccaaa tattgctaaa caaactgctc acatacaatc aacaggttgt aatgcacagg
    14461 taaaaatggt taatagtcaa attgaagcgt atgcattgaa acataataga aatccatcgt
    14521 ctattgaaga cttaattgca gatggtttta taaaagaagc acaaaagaca tgtaaatcag
    14581 gagagacaat cacaattagt aatggagaag cagttgcaaa ttagaaagca gtcagcattt
    14641 actatgattg agatgcttgt ggtaatgatg ttaatcagta tatttctact tttgacaatg
    14701 acatctaaag gattaagcaa tcttagagta atagatgatg aggcaaatat catttctttt
    14761 attactgaat tgaattatat taagtcgcaa gctatagcaa atcaaggata tatcaatgtt
    14821 agattttatg aaaacagtga cactattaaa gtaatagaga ataataatat acgatttcta
    14881 aaattaaaag taggcaaaat aattaatgtt gcaaaagttg atattattgc ctttgataaa
    14941 aaagggaata tcaataaatt tggtagcata acaatttaca ataacaattc aatttataga
    15001 ataatattcc atattgaaaa aggaagaatt cgttatgaaa agctataagt gtaaaggttc
    15061 attcttaata gatagtatgg ctggattttt gctaattgga ttgattacat tactattgat
    15121 accaatgatg aatcaaatgc aagcgagtat aaaccataaa ctacaaacaa ttgatgcttc
    15181 taaagtaatt ttgacgactg tatctaaaat taataaagaa gaacttaaga agggggtaac
    15241 tatagggaag tatgatatta agcaaagtga ccaacaaatt tgtgctattt caaaaaatac
    15301 cacttcttat caaaagacat gtatacagta ttaatgtcaa agctttttcg ctcattgaaa
    15361 tgttagtagc gatgatggtt ataagtataa ctttactaat tgttccagac ttaattagac
    15421 ttagtaaaac ttttctaatt gaaagtaggg atttaacaac tgtagatttc gaatttttct
    15481 caagagatat tctagatgat tttaaaggag tagatagaaa cgatattgaa attaggcaac
    15541 accgtatcat tttacataaa ggtgaagaaa tgatcgaata caaattaatt aataataaaa
    15601 ttattaaagt tgtaaatgac agaggaaata taacaatgat taataatgtt acagcattta
    15661 ctgcaaatat ctactataaa tccattatta aaataacgat aacagttaaa gtcggtacaa
    15721 atgtgcagac taaaactatt tatgtataac ttactagta
//
LOCUS       DALAGF010000069        15350 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.069, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000069 DALAGF010000000
VERSION     DALAGF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15350
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.069"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(62..397)
                     /locus_tag="O2692_001877"
     CDS             complement(62..397)
                     /locus_tag="O2692_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4299502.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            complement(486..1745)
                     /locus_tag="O2692_001878"
     CDS             complement(486..1745)
                     /locus_tag="O2692_001878"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCZ4299503.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            complement(2211..3071)
                     /locus_tag="O2692_001879"
     CDS             complement(2211..3071)
                     /locus_tag="O2692_001879"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCZ4299504.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            3289..3810
                     /locus_tag="O2692_001880"
     CDS             3289..3810
                     /locus_tag="O2692_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HCZ4299505.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            complement(3919..5529)
                     /locus_tag="O2692_001881"
     CDS             complement(3919..5529)
                     /locus_tag="O2692_001881"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCZ4299506.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(5865..6395)
                     /locus_tag="O2692_001882"
     CDS             complement(5865..6395)
                     /locus_tag="O2692_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCZ4299507.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            complement(6507..7367)
                     /locus_tag="O2692_001883"
     CDS             complement(6507..7367)
                     /locus_tag="O2692_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4299508.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            7791..8594
                     /gene="coaW"
                     /locus_tag="O2692_001884"
     CDS             7791..8594
                     /gene="coaW"
                     /locus_tag="O2692_001884"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HCZ4299509.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLEK"
     gene            complement(8724..9395)
                     /locus_tag="O2692_001885"
     CDS             complement(8724..9395)
                     /locus_tag="O2692_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HCZ4299510.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(9794..10987)
                     /locus_tag="O2692_001886"
     CDS             complement(9794..10987)
                     /locus_tag="O2692_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HCZ4299511.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            complement(10987..12171)
                     /locus_tag="O2692_001887"
     CDS             complement(10987..12171)
                     /locus_tag="O2692_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCZ4299512.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGR
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            12482..12952
                     /locus_tag="O2692_001888"
     CDS             12482..12952
                     /locus_tag="O2692_001888"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCZ4299513.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            complement(13104..13454)
                     /locus_tag="O2692_001889"
     CDS             complement(13104..13454)
                     /locus_tag="O2692_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299514.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(13468..14769)
                     /locus_tag="O2692_001890"
     CDS             complement(13468..14769)
                     /locus_tag="O2692_001890"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCZ4299515.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIGGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
     gene            complement(15048..>15350)
                     /gene="deoC"
                     /locus_tag="O2692_001891"
     CDS             complement(15048..>15350)
                     /gene="deoC"
                     /locus_tag="O2692_001891"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCZ4299516.1"
                     /translation="VKVIIETVLLDHDEIVKASELTKTAGADFVKTSTGFAGGGATAE
                     DVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADSDY"
BASE COUNT         4824 a         2986 c         2105 g         5435 t
ORIGIN      
        1 cgcaaataat gcgaagactt agaaatagtt ataatcaatg ctatgtttaa acgtcatgta
       61 attatttagc agtacgttga tctgttaaat cgacatatga ttgcgcccat gcttcaattg
      121 gatgtaaagc ctctgctaac gctttacctt tttcagttag cacataaata atttgtactg
      181 gactcgtaga aatgatttgc ttctcaacta actcccattg tgcaagctct gacaacttaa
      241 gacttaaagc acgtggtgtt attgttttca aatctctttt catatcggaa aagtgtgctg
      301 aacagtcatt acatcttgag agataattaa tgattaatcc attccaactt ctaccaagta
      361 ttttaaaagt ttcttcgaga tacggacata cttccatcat cttcacctct catcaataaa
      421 tttagaatgt aaacgaacac cattttttaa tcagtataat tattatacca caaaacaaga
      481 taaatctata cagtttccgt ccaaatatct gcacctaaag cttttaagtg ttctacaata
      541 tctgtataac ctctataaat atgtttaaca ttgtaaattg tagttacacc ctcagcaatt
      601 aaaccagcaa taattaaaca agctcctgct cttaaatcgc tagcataaac ttcagcacca
      661 tgtaatgttg atggttttat cgttgctgtg ccttcgtcaa cttcaatatt tgcacccatg
      721 cgctttaatt cttcaacatg tttaaaacgc tccggataaa tcgtatcagt tacaaatgaa
      781 ggaccatttg ccataaataa taatggtgta ataggctgtt gcaaatcagt agcaaaacct
      841 ggatatacta gtgttttaat atcaacaaat tgatatggcg cattattatt gatgcgaatt
      901 ctttcgtctc ttacatcaac attcacacct aattcactaa atttagcagt taatgtttct
      961 acatgtttcg gaacaatatt atttaatata acattttctc cacatgctgc agcgatacac
     1021 atatatgtgc ctgcttcaat tctatcaggt ataacttgat actcagaacc atgtaattct
     1081 ttgacgccat tgattttaat tgttgatgta cccgctccct taatattagc tcccatactt
     1141 gttaagaagt tagcaacatc aactacttcc ggttctttag cagcattttc aattacagtt
     1201 tgtcctgttg cataaactgc agctagcata atgttaattg ttgcacctac gctaaccata
     1261 tctagaaaaa tatgtgcacc ttttaactct ttagcttcga ttttcattga tgtcgtactt
     1321 gattcatcaa tttcagcacc taaagcttta aatcctttaa tatgttgatc aattggacgt
     1381 ggcccaagtg gacatcctcc cggtaaacca atcacacatt ttttaaatct acctaacatg
     1441 gcacccatca tatagtatga agcacgtaat gattcaactt tattatttgg taatgcagca
     1501 ttttgtattt cagttgtatc aacttctaat tccgtaccat ttagtgatgc cttaatattt
     1561 aaatcttcta ataaactcac taaagtttta acatcagaga tttgcggtaa cccttctaat
     1621 ttcacatgtc cttgagctaa taatgttgca ggaatgatag ctacagcact gtttttcgcg
     1681 ccactgatat taacttcccc atttagtgtg cgtccacctc ttatttttat tacctcttga
     1741 gccatatcat ttatctcctt tgtccattac tttttaattt tttgcattat tagcattata
     1801 aattaatttt actttatttg taaaatagtt tattttaatc gttaaaatct tgtctttcta
     1861 ttttcgattg attaataaca aatcattcac ctttactaat atatgctgtt cttccccact
     1921 tgaccatggt ctaaagtctg attattcaac taaatattga cgttgttttg acaattattt
     1981 agttttaatt ctacggtttt cacgtaaact tttttcataa attgctcatt ttccttataa
     2041 ttatatcaca atcaattttg atatatttta ccatttttac gctttcattt taaaatcaaa
     2101 ctgttttatt tattacttat acgacataaa aaagaagcaa acaccatttt gtctgcttct
     2161 tttaaaatta atatccctac gttattaata acttaaagac taaatattaa ttatttagcg
     2221 cggttagaag taccgaactc tttaatttta cctttaactg tttctttgat ggcttcacgt
     2281 gcaggtccta agtatttacg aggatcgtaa acttctttgt cgttatttaa aacgtcacga
     2341 actgcttttg ctgaagcgat ttggttttca gtgtttacgt taattttagc tgtaccaaat
     2401 ggaattgctt tttggatatc tttagtcggg ataccagtac caccgtgtaa tactaatggt
     2461 aaacctgtag ataaaccgat ttcttccatt tctttaaatc ctaattttgg ttcacctttg
     2521 tatggaccat gaactgaacc taatgctggc gctaatgcat caataccagt tttttcaact
     2581 agttcttgac attctttagg atcagcataa atgatgccgt ctgctacaac atcatcttct
     2641 tgtccaccaa cagtacctaa ttcagcttct acagaaacac ctttttcatg agcgtattca
     2701 acaacttttt tagttgttgc tacgttttct tcgaatgggc tgtgtgaagc atcgatcatt
     2761 actgatgtga aaccagcatc gatagcttct ttacattttt caaagcttga accatggtct
     2821 aaatggattg ctacaggaat agtgatgttt aagtcatgca ttaacccttc aaccatttta
     2881 acaattgtgt agaaaccgct catgtaacga gcagcacctt cagaaacacc taaaattaca
     2941 ggtgcatttt cttcttgtga cgcttctaaa attgcttgag tgaattctag gttattaata
     3001 ttgtattgac ctaccgcata accattttct tttgcatcaa ttaacatttc tttcattgaa
     3061 actaaaggca tgaaagttcc tccttgagtg cttacgcacg tatttttcaa agtaattata
     3121 tcaaaaaaat agcgtcaatg cattgaaata ctgcagatta aatgtgtatt ttttgaaaaa
     3181 tgcgatagtt atactaaatg caaacgtata catttttata caatttaagc cgttgcataa
     3241 tatgtagtca ttaattacaa tattttataa gtagattgga ggataaagat gtcaaaaatc
     3301 ttaaacacac aactaatagg tatctttaat aggctagaaa aacaatcttt agaaattcaa
     3361 atggccgccc aatgtttaat tcaagcaatt ggtggcgaag gttatgttta tgtaaaaggt
     3421 tatgatgatt tacagttttt cgaaagtttc attctacata gtgatgagcg tttaaaatct
     3481 agccgaaaac ttgacgctat taaagacttt aaagaaattg actcaactga tcgtgtatta
     3541 ttattcgcac cattttataa tgatcaagtt gcattagata ttcaaaaact tatcgactta
     3601 gatatcgatg tggtcttaat aagcaacaaa cctaaaacag atgatttccc tgatcatctc
     3661 gtacatttta tcgatttatc tacaccaaga cctatagtct acactgaaga ttacgataag
     3721 attgtccaac cacatgcgat ggcattgaat tatgtatact atgacattta tacacaaatg
     3781 attgaaatga caagagactt agaattataa aagtcactat tgttatttca taccttgtca
     3841 ctaacataat attaaaatta tacgaaaaac cggtactttc aatgtttaac aatgaaagta
     3901 ccggttttat tagcaaattt atttattttg ttgatatttt aatgaagctt caataaatga
     3961 tttaaaaatc gggtgcggac gatttggtct agataagaat tctgggtgga attgacaagc
     4021 aataaagaaa tcatttgtcg gaatctctac catttctact aaacgtccat ctggacttgt
     4081 accagaaatc accataccat ttgcttctaa ttgttctcta tagtcattat taaattcata
     4141 acgatgacga tgtctttctt caatttccgc tttaccataa acatcttgtg ccaatgtgcc
     4201 ttctttaatt gaacatggat ataagcctaa gcgtaatgta ccacctaaat cttcgatatc
     4261 tttttgttct ggtaataaat ctataattgg gtatggtgtt gctgggtcta attcagctga
     4321 atgtgcgcct tcaaggccta atacgtttct tgaaaattca actgttgcaa gttgcattcc
     4381 taaacaaata ccaaagaatg gtacattgtt ttctctagca tacttaattg cactaatttt
     4441 accttcactt gcacggaaac caaatccacc tggtactaaa ataccgtcga catctgcaag
     4501 gtattcggct gcattttcat ctgttacttc acttgaatca atccatctaa tgtcaatatc
     4561 tttggcaaaa ggatatccag catgtttcaa tgattcaaca actgataaat atgcatcttg
     4621 taagctaaca tatttaccta ctaaaccaat tgtaatttta ccatctaaat tattaacgat
     4681 atctaacaac tgtttccatt catcaagctg tgtttcatat ttcgcgttta attgtaaacg
     4741 tttaataacg atatcatcca tattttgttg gcttaattgt aatggaattt cgtataaaga
     4801 gtctgcatca cgacattcaa taacactttc tttattaatg tcacagaata atgcaatttt
     4861 atcttttaaa tcttgtgtca tttcatattc agttcttaca acgattaagt ctggttgaat
     4921 acctaagcct cgtaattctt taacactatg ttgtgttggc ttcgttttca tttctccagc
     4981 agctttaata taaggcagta atgtacagtg aacatacata acattttctc tacctaaatc
     5041 gctacgaatt tgacgaatcg cttcaataaa cggtaatgac tcaatatcac ctgttgtacc
     5101 gccaatttca gtgataacaa cgtctgcatt cgtactttcc cctgcaagta ataaacgttc
     5161 tttaatttca tttgtaatat gcggaataac ttgaactgtt ccgcctaagt aatcaccacg
     5221 acgttctttt ttcaatacgt gtgaatacac tttaccggct gtcacatttg aaaacttgtt
     5281 taaattaata tcaataaatc tttcgtaatg tcctaagtct aggtcagttt ctgcaccatc
     5341 atccgttacg aatacttcac catgttgata aggactcatt gtacctgggt caacatttaa
     5401 gtatggatcg aatttttgaa ttgttacatt tagacctcta tcttttaata atctacctag
     5461 agaagatgct gtaataccct tccctaatga tgaaactacg ccacctgtta caaaaataaa
     5521 ttttgtcatg tcttgacctc ctaattatta aagggaatgc ttcctcatca attgtaaaaa
     5581 ttacgtaaaa cataacatta aagcaagctc attcaagttg tttatttgtc gaattcgaca
     5641 aaattaaatt ctgtttactt accgtttcca tactgctatg tcactatgta tatcatttac
     5701 aactttgatt tttgcgttat catttcttga tttactcaat taaataaaaa aacgctccct
     5761 ctcacaaatt ctgtaagggg agcgtataaa ttttatacac gtgtcctatt taaaggagcc
     5821 cgaataaaat tttatcatct ttcaactaaa agtcaaacaa aaaattaatc gttgaagtct
     5881 tcttcgtctt caaacacttc ttcgtcttcg tctagttcat cttcatcttc ttcaatgact
     5941 atatccgaat gattgatttc ttcttcaacc tgctcatctt ctggatcatt tagttcttct
     6001 tgatcatctg tttgagctgg aatatcatcg tcgtcatcca tttcatcttc gcccaataat
     6061 tttaagtttt gatcttcttc atcatctgca tccagaatat cgaatttttg aatagttggt
     6121 gcgattttct cttcaatatc atctaccgaa taccaatcac gtaatcccca taaattttct
     6181 ccaacattta aaaaacgacc atctgtgttt aaatccgtgt aaaattgtac aacacgattt
     6241 tcaatttctt cgtactcata atcacctaac gctctaaatt catcgatgat atcatataag
     6301 ttcattgttt cgcctttatc attcaataat gtataagcca tatcaataaa tgatttttca
     6361 tcaaccattt gttttgtata atcttgaatt ttcattgccc aatacttcct ttcgaaggat
     6421 ataatctaaa tttatacgtc tgaattcatt tatatatagt aacaaaacta aattataaat
     6481 gacaatatca tatttcgcaa tcactgttag taatttaaat atttttcgaa aacgacataa
     6541 tcatcagttt gttcgatttc aacgaaatta ctatttaaac ataatggttt aaaatcatta
     6601 ttctctccaa acagaatcat cgtaatcaca ttaaggttgt catagttttt tcttaagtaa
     6661 gttggcaatt gtcgcagagc acttgaacca ataccattaa agcgatatga tttatccact
     6721 tcaatagcag caatgttcat gcgttcacca atctgctcca atgcgataaa gccaaccttt
     6781 tggccatcaa tcattaaatt aatgatatgt acattggtca cttctgaacg actttcttct
     6841 tcaagcttta atgatgaaac atgttttgaa tctaagtcca agtaatatag tcgttctttg
     6901 ccaattggtc cttcttcttt agtcgctgta tgcatgaatc cagcacgttc gtataggttc
     6961 caagcattgt cattttccgc atctactact agatatagat gattaaaatc aggaaataca
     7021 ccttgaacat attgcggcaa tgacatcatt attttcgtgc catatccaaa accttgatat
     7081 ttttcattaa tcgataatga acgaatataa acgacatttt caggtgtatc ataaccttca
     7141 tgctgatagt aacgatgtaa tacaaaaaag cccaccactt catttttatc gtttattgct
     7201 acgttagcaa tcctatcagc atcattaatt gcatcatcaa taacttcttt aggtaaagat
     7261 gaatatattt gttgtctttc attcaagtca aaatcattaa ttgctgaacg atattgttca
     7321 tcaaatgctt ttaaagtgat accttcaaaa caaagttctt tagccatata tttaacccct
     7381 atctttttat atatgaagca tgaatcccca tattatttta cctattattt cactacgata
     7441 ccattatttg ctctaagtta aataaattac ttatttatat tttttaaatt tcaaaattaa
     7501 tgtactattc ctcattactt atcattttaa tcagagactt acaaatacac tgcgtacgaa
     7561 ctacttttaa tattgcgtca ctaactaaca atcctataca acttcaaaat tcctagaaaa
     7621 taatatatat aacttaacgt ccacgtttgt aaaacattaa ttgcgatacg cttacaatgt
     7681 tgaaatgcaa tggcatcaaa tttgttaata attactatta ttacttgttt tttagtcatt
     7741 ttactatact attatttaag gatagacatt aataatgagg tgggaatctt atgaaagttg
     7801 gcattgacgc tggcggtaca ctaattaaaa tcgttcaaga gcaagataac caacgtactt
     7861 ttaaaactga attaactaaa aatattgatc aagttgtgga atggttaaac caacagcaaa
     7921 ttgaaaaatt atgcttaact ggaggtaatg caggtgtcat tgctgaaaac atcaacattc
     7981 ctgcacaaat ttttgtagag tttgatgctg catctcaagg actaggtatt ttgttaaaag
     8041 agcaaggcca tgacttagca gattatattt ttgcaaatgt tggtaccggt acgtcactac
     8101 attatttcga cggccaatca caacgtcgtg taggcggtat cggtacaggt ggaggcatga
     8161 ttcaaggact cggttattta ctaagtcaaa tcactgatta taaacagtta actgacatgg
     8221 cacaacacgg tgatcgtaac actatcgatt taaaggttag acatatttat aaagataccg
     8281 aaccacccat tcccggtgat ttaacagcag ctaattttgg tcatgtacta catcatttag
     8341 atgctgattt tacacctagc aataaattag cagccgttat tggtgtcgtt ggcgaagttg
     8401 ttacgacaat ggccattact gttgcacgtg aatttaaaac tgaaaatatc gtttatatcg
     8461 gttcttcatt tcataataac gctttattac gcaaagttgt ggaagattat actgtgttac
     8521 gcggttgcaa accgtactat gttgaaaacg gcgcattttc aggtgctatt ggtgcattat
     8581 atttagaaaa atagaaaaag accatttata tgcgcgttgt tttaaataaa cgctatataa
     8641 atggcctttt attttatggt ctaagtgttt tgaatacgta ttgttccatt tattatcact
     8701 taattactta caactcaatt caattaaaat gtaagttcat tagacaattt ttgcgctgaa
     8761 acaattgtac caaccacgcc agtttttaaa tctatcgcaa cggctttatg atcatctcta
     8821 agttcatcta gccattctcc gaaaacagaa ccttcaatcg taattaaatc acactcttca
     8881 aaatcttcat ctcgaacttt taacgctctg tttttaatac tccatacagg cataatatgc
     8941 ggcttttttt cgtcgctatc attttcacta atgagaataa actgcttact acctttttta
     9001 gttaagccgt atacttcatc atatttcgct acatcttgga caaattcttt gattctaatt
     9061 ttatctaatt ccgtcatcac ttcttgtgta acagctacaa cgtttagtag atgcccttct
     9121 tcttccattg tttgattcat aataatatgg tcttgttcat caaacaattc atctaattca
     9181 taagtagcaa aacgatcaac gtccattttt acaactttat caaatggaat atcacgactt
     9241 gttaaaaatg atacggccac actttcttct gtccaaacgc ctacatacgc ttcactttta
     9301 taatttaggc gtactagttt tttattttta gaggcgaaat aaatacattc atttattaat
     9361 atatctctaa agaatggata tgctttataa gacatgcttg accctcactt tttaaaatat
     9421 gtcatacata aaatagtaaa tgactccaac aagttagtca attagacata ttatgaattc
     9481 aatttaatgt catgtttttc attttaaaaa ttcaatagca ttaaatttta cgtatacaca
     9541 aattcggtct ataacacgaa acatatctag ttactaaaaa tttcaaaacg ataaccttta
     9601 gtataccttt atcaacaaaa tggcaactct tataacatag tgaaataaaa gtcgccattt
     9661 aatattagta ttacaaagta ttgccatatc ataaatttaa ttactgttga ctagaatcga
     9721 aaaaagcttg ttacaaacgc atattcagtc aactactggc aacacaacat tgtagcgcct
     9781 tatgttccga actctaatag tgttcaagtt cagctaaaaa tgcgcgttca ttctccagta
     9841 ctgtttcttt tgaatcacca tgccaaatac agccaaacct tgatttaact ttatcttcac
     9901 cgtaccaatc accatcataa taagataacg ggtataaact accttctttg acatatttca
     9961 aaatcgtatt gaagaaatgt gtgttatcac cttttgaatg ataactataa aacttttgat
    10021 atcctgaatt caactcgtta gcaagtaata acatgctcgt tgaaccattt tgtctaaagt
    10081 ttaaatcaat cgcataaacg ttatcatcct catcgacgag taggtcaaaa ccagctacac
    10141 caaagaatcc ttgattcaca ccgttttcca taatttgtct acctgcttct ataacgtgct
    10201 ctggtacatt agtagtattt tcatttccgt tataaaatcc gtacttatcc gttaattgcg
    10261 ttgccgcacc aagatattga atgcctagcg attccgagta tgcaaactga acgcaataat
    10321 tcgctttttc ttcaatcttc tgttcgataa taagcgaatt cgtttcagca gttgcctctt
    10381 taatacgcgt tatcgctttt tgtaaatctg catcatgata acaaatcata acgccatagc
    10441 cacctgctgt cggaagatca tctcccggct taattactaa cgggaattcc caattcttaa
    10501 tctcgttttc gaattgctca atttttacaa cttttctttt tggtaaaaac ttcccatttg
    10561 tccattcagg tattcttgct ttattattta aagcaacaaa taacgtttta tctaatgcat
    10621 aatattgctg attcaagatt gtttcatcat gaatatattg aaaataaatc tttttatttt
    10681 ccttatgtgc caattgtttg atcaagtttt cgtaagattg ctgattgtta aatgtataaa
    10741 ttgagttcgg tacttcctta ccaataactt gaaatagctg atgcaatttg tctgtcgcac
    10801 tagcttcgtg aacaataaca ggtaattgat ttgctattaa taactcccta ccagttaaaa
    10861 aattagattg atgttcgtcc ggtttcaacc atggattcga tatatacgaa ggtcttgacg
    10921 tatagacaac atctttgtca tataaatcac ttaacgttaa gttcggctca ttaccattat
    10981 ttgtcattac ttcccattcc ctttcaaatg cgcatgctct tcaataatgt cttgataaac
    11041 gtcttgattt gtaattaact ctaaccccat caacgccatt attttagcgc ctttaattaa
    11101 tgcttcatca ccatgtacac tcgcagccgc ttctctaaat ctatgcgtat gtcctactaa
    11161 attacgtgat cctattttaa tatgaggatg tattgttggc acaacatgac ttacgttccc
    11221 tgtatccgta gagccataac caaaatcatc atcaataact gcttcaccaa cttcttcagc
    11281 atatttagca aataaatcat ctaatttcgg cgttttaatg aattcattca caccgttttg
    11341 aattcgacca aattcataat cacaaccagt ctgtatcgca gctccacgtg cgatttgatt
    11401 tactttttct gttaatatat ccaattcttt acgcgtcatt gctctagtat aaaaacgagc
    11461 atgtgtatag tctggaataa tattagctgc tttcccgcca tctaaaatca caccatgcac
    11521 acgttgatct tttttaatat gttgtcgtag ttgtgctaca ccattaaaat aactaatcat
    11581 agcgtctaat gcatttaacg cttcatctgc attttcagag gcatgagcac tttttccgta
    11641 aaatttaaca tctaaaacat ccactgccaa agtatcaatc gttttataag tttcatttcc
    11701 cggatgaatc attaaggcaa tgtctatttg atcaatcaca ccagccttga cataagaagc
    11761 tttagcgcta ccattttccc caccttcttc agctggacat ccaagaacga ctactttacc
    11821 accaatttgg tcaatcactt gcttcaaacc aattgcacca agaacacttg cagttccaat
    11881 gatattatga ccacaagcat gacccaatcc tggcaaagca tcgtattctg ctaaaaaacc
    11941 tatagctggc ccgtcaagtc ctgaatcata tgtcgctata aacccagttg catgcccagc
    12001 aatctcggtt tcaatttcaa aatcatgctc tttcaaacga tctattaaag ttcgagacgc
    12061 aaatatttct tcattaccaa gttcaggacg ttcatgaatt ctatgactga tttcgatata
    12121 actatattta tttgtttcta tataatcgag aatttgttgt ttttcactca ttttttacta
    12181 tctccctttt accctacact cattttaatc atccacaata ttttgttctt tcaaaatgaa
    12241 ttattactta ttctatcggt tttatctcat gatgtcatct agtttttctt tatttacaaa
    12301 attttctaat aactaaaagc ctttcataaa tttataaaac agtttcaaat tgtaaaacat
    12361 aagccctatt gttacaattt aagtattgtt agaaacacaa cttacaacaa acttaagttt
    12421 tataatgcaa caaatcataa gcgttttata gttttgagta gttaatagga ggaaattcaa
    12481 aatgacaaaa atgaatgttg aaagtttcaa tttagatcat actaaggtgg ttgccccatt
    12541 tattagatta gcgggaacga tggaaggatt aaacggagat gtcattcaca aatacgacat
    12601 tcgtttcaaa caaccaaaca aagaacatat ggatatgccc ggactacatt cattagaaca
    12661 tttaatggct gaaaatatta gaaatcatag tgacaaagtt gttgatttaa gtcctatggg
    12721 ttgccaaact ggtttctatg tatcatttat taatcatgat aattatgatg atgtattaaa
    12781 tattgttgaa gcaactttaa atgatgtgct aaatgctact gaagtgcctg cttgtaatga
    12841 agtacaatgt ggctgggcag caagtcattc attagaaggt gctaaaacta tcgctcaagc
    12901 atttctagac aaacgaaacg aatggcatga tgttttcggt acaggaaaat aaatcttagt
    12961 caatcaagtt aatcagaaaa gcagtcgaac aatgatttta caatcgccat tgtccaactg
    13021 ctttttatta tgcttcaaag tcaaaaaatc gaacaaatga aaaagtaaaa tctttaacat
    13081 ttgtccgatt tatttgagaa ccactataat ttcttaatta gtcccattaa cacgaactgc
    13141 ataggtaacc ttaaatatag ttgccatgtt ggcaattgtt tatctcctaa aggtaacttt
    13201 ttaactgcca tatagatatt agctgggaat acagctagca agaatagatt gattgtattt
    13261 ttcaagcatt gagatggtct tttaattaaa agtgcaagtc caaataatat ctcaaagatt
    13321 cctgtaacaa gaaccgctgt ttttcgaagt ggcaaacatt tcggtatgat atttctaaat
    13381 tgtcgttctc gtgtaaaatg caatacacct attacactaa aacctattcc taataaatat
    13441 cttagtatgt tcaatcagct tcaactccta ttctgtaatg attttatgaa ttaatgtagg
    13501 cgatacaaca tgatcagcaa ttgttatgct tgaatctaat tttttaacaa catcgtctac
    13561 atcttggcga ttactgtgaa tcgttaataa tgattctcct tcttctactt tatcaccaat
    13621 ttttttattt aaaacaatac caaccgctaa atcaatatca tcctcttttg ttaaacgtcc
    13681 cgctcctaac atcatcgaag cgacacctat atcgttagag actaattcag tcacataacc
    13741 tgatttttta gctttatatt caatttgata ttgagcttgt ggcaaacgct ctggatggtc
    13801 aataacagtt tcgtcgccac cttggttttt aataaatgtt ttgaattttt ctaatgctgc
    13861 acctgaatta attgcctcaa ttagcaacgc tctcgcttct tcaagcgttt cagctttgtt
    13921 tgcaagtaca accatttgag aacctaatgt taatacaagt tctgttaaat ctttcggacc
    13981 ttgtcctttc aacgtatcaa ttgcttcttg taactcaagc gcattgccaa tcgcacgtcc
    14041 aagtggctga ttcatatcag aaataatcgc catcgtatta cgtcccacat tattaccaat
    14101 acgtaccatt gcgtgcgcta atgcttcagc atcttctaat gttttcataa atgcaccgct
    14161 accagttttt acatctaata caattgcatc tgcaccagca gcaatctttt tactcataat
    14221 tgaagaggca attaatggta ttgaattgac agtaccagta acatccctta aggcatataa
    14281 ttttttgtct gcaggagtta aatttcctga ttgtcctaca actgccactt tattttcatt
    14341 aaccaatttc acaaatgttg cttcatctat ttcaacatga aaaccatcaa ttgcttctaa
    14401 tttatcaatc gtaccacctg tatgacctaa tccacgccca ctcatttttg caacaggaac
    14461 atctacagct gctactaatg gtgctaaaac caatgtagtt gtatctccta caccacctgt
    14521 tgagtgctta tctactttga cacctttaat atcactcaaa tctatcatat caccagaatt
    14581 aaccatagcc atcgttaatg ctgcacgctc atcatcattc atatcttgga aataaatcgc
    14641 cattgctaaa cttgatgctt ggtaatcagg aatatcccct ttaacatagc cgccaataaa
    14701 gaaattaatt tcttccgttg ttagtgtatg accgtcacgc tttttctcaa taatgtctat
    14761 cattctcatt tttatcatcc ttttcttaaa aagcttagga caaagcatct gcgctttctc
    14821 tagtccattt ttaaaagcac aagcgaaaat tattatagca agctatcgat ttcattttta
    14881 atatcacaat ttcaatgcga tgttattatt cttaaataga ttggttataa cgttaaagtc
    14941 cctattaaat tatcttagaa tcatcatggc atttatgatg tcttaaagct gatatcgaca
    15001 tacttatata tggttacgat gtcccatgct tacatatttt tataaaatta gtaatctgaa
    15061 tctgcttcta aaccttgcat aatttgaacg cctgcgctcg caccaatacg tgtcgcacct
    15121 gcttcaacca ttttattgaa atcttctaaa ttacgtacgc cacctgatgc ttttacttct
    15181 acatcagcac ctactgtatc tttcattaat ttaacgtctt ctgcagtcgc accgccacct
    15241 gcaaaacctg ttgaagtttt aacgaagtcc gcaccagccg tttttgttaa ttcactcgct
    15301 tttacaattt cgtcatggtc caacaatacc gtctcaataa tcacttttac
//
LOCUS       DALAGF010000070        14837 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.070, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000070 DALAGF010000000
VERSION     DALAGF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14837
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.070"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..353
                     /gene="fmhC"
                     /locus_tag="O2692_001892"
     CDS             <1..353
                     /gene="fmhC"
                     /locus_tag="O2692_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HCZ4299517.1"
                     /translation="TEELIFEDGPVLDLAAALFICTDDEVYYLSSGSNPKYNQYMGAY
                     HLQWHMIKYAKSHNINRYNFYGITGVFSNEADDFGVQQFKKGFNAHVEELIGDFIKPV
                     RPILYKFAKLIYKV"
     gene            526..1398
                     /gene="dprA"
                     /locus_tag="O2692_001893"
     CDS             526..1398
                     /gene="dprA"
                     /locus_tag="O2692_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCZ4299518.1"
                     /translation="MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFPLLLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
     gene            1572..3647
                     /gene="topA"
                     /locus_tag="O2692_001894"
     CDS             1572..3647
                     /gene="topA"
                     /locus_tag="O2692_001894"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCZ4299519.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            3803..5110
                     /gene="trmFO"
                     /locus_tag="O2692_001895"
     CDS             3803..5110
                     /gene="trmFO"
                     /locus_tag="O2692_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HCZ4299520.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
     gene            5527..6423
                     /gene="xerC"
                     /locus_tag="O2692_001896"
     CDS             5527..6423
                     /gene="xerC"
                     /locus_tag="O2692_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCZ4299521.1"
                     /translation="MNHIQDAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLEL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKNLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            6420..6965
                     /gene="hslV"
                     /locus_tag="O2692_001897"
     CDS             6420..6965
                     /gene="hslV"
                     /locus_tag="O2692_001897"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCZ4299522.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            7031..8434
                     /gene="hslU"
                     /locus_tag="O2692_001898"
     CDS             7031..8434
                     /gene="hslU"
                     /locus_tag="O2692_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCZ4299523.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            8459..9232
                     /gene="codY"
                     /locus_tag="O2692_001899"
     CDS             8459..9232
                     /gene="codY"
                     /locus_tag="O2692_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCZ4299524.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            9283..9375
                     /locus_tag="O2692_001900"
     CDS             9283..9375
                     /locus_tag="O2692_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299525.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDISH"
     gene            9574..10341
                     /gene="rpsB"
                     /locus_tag="O2692_001901"
     CDS             9574..10341
                     /gene="rpsB"
                     /locus_tag="O2692_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCZ4299526.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            10375..10488
                     /locus_tag="O2692_001902"
     CDS             10375..10488
                     /locus_tag="O2692_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299527.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            10523..11404
                     /locus_tag="O2692_001903"
     CDS             10523..11404
                     /locus_tag="O2692_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCZ4299528.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            11541..12263
                     /locus_tag="O2692_001904"
     CDS             11541..12263
                     /locus_tag="O2692_001904"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCZ4299529.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            12282..12836
                     /gene="frr"
                     /locus_tag="O2692_001905"
     CDS             12282..12836
                     /gene="frr"
                     /locus_tag="O2692_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCZ4299530.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            13209..13979
                     /locus_tag="O2692_001906"
     CDS             13209..13979
                     /locus_tag="O2692_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCZ4299531.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            13986..14768
                     /locus_tag="O2692_001907"
     CDS             13986..14768
                     /locus_tag="O2692_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCZ4299532.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
BASE COUNT         5568 a         2057 c         2836 g         4376 t
ORIGIN      
        1 aaaccgaaga actaatattt gaagatggac ctgttttgga tttagctgct gctttattta
       61 tatgtactga tgatgaagtt tattatctat caagtggatc aaatccgaaa tataatcagt
      121 atatgggtgc atatcatcta caatggcata tgataaaata tgcaaaatca cataatatta
      181 ataggtataa tttttatgga ataacaggcg tctttagtaa tgaggcggat gattttggtg
      241 ttcaacaatt taaaaagggt tttaatgcac atgttgaaga attaattggt gatttcatca
      301 aaccagtaag accaattcta tataaatttg caaaacttat ttataaggtt taattataaa
      361 gtatgttgga aattgaaatt ttaaattctt tccaacatac ttttcacttt ttaagtcagt
      421 atttttactc cttaaaataa cattacatcg acaatgtttt tgtattagaa atgtaaaaca
      481 aaaatagaat atgattttgt atgattacaa aaaggagttg atttattgat taaactattt
      541 ttgcttaagt tatactgggc acacttttcg actaaacaaa ttcatcaatt tttaatggca
      601 tatcctaatg taattaaaga ggagggaaga aaaaaagata gttatttatg tgaatgggtg
      661 aatagggaag aaaatgttca tttattacgt aaatactatg cttttataaa acttgatcat
      721 aacgatatta ttaaagaact gcagaaatta aaagtaagtt acattacata tatggattct
      781 gaatacccag tgctattaaa agaaatatat caatttccat tacttctttt ctataaaggg
      841 aacatcaaat taataaataa tatgcatcat ttggcagtag taggtgcaag agattctaca
      901 agttataccc aacagtcttt agaattttta ttatcaaatg ataaaagcaa atatttaaca
      961 attgtttccg gccttgctca aggagctgat gcaatggcac atcaaatagc tttaaaatac
     1021 aatctcccta caattgcagt tttagccttt ggccatcaaa cacattatcc caaaagtaca
     1081 ttagcattaa gaaataaaat agaagaaaaa ggtttagtta tatctgaata tccaccacat
     1141 acaccaatcg ctaaatatag atttcctgag cgcaatagaa ttatcagcgg tttgtcaaaa
     1201 ggggttttaa ttactgaggc taaggaacaa agtggcagtc acatcacgat agattttgca
     1261 ttagagcaaa atagaaatgt ttatgtttta cctggatcta tgtttaatcc tatgacaaaa
     1321 ggtaatttat tacgtatcca agaaggtgct aaggtagtat taaacgctaa tgatatattt
     1381 gaagactact atatttaaaa ctaaaaataa cgagttgctt catatttctt ttgtttccct
     1441 ttgtaattta ttttttaata aaatctataa aaaatagaca gggaaaatga tttgtttaga
     1501 tataaaacgt tgacaaaagc aaaattaagc gtttatcatt tatctttagt aattagatta
     1561 gcgaggggga aatgacattg gcagataatt tagtcattgt tgaatcgcct gcaaaagcaa
     1621 aaaccattga aaagtattta ggtaagaaat ataaagttat agcttcaatg ggacacgtca
     1681 gagacttacc aagaagtcaa atgggtgtcg acactgaaga taattacgaa ccaaaatata
     1741 taacaatacg cggaaaaggt cctgttgtaa aagaattgaa aaaacatgca aaaaaagcga
     1801 aaaacgtctt tctcgcaagt gaccccgacc gtgaaggtga agcaattgct tggcatttat
     1861 caaaaatttt agagcttgaa gattctaaag aaaatcgcgt tgttttcaac gaaataacta
     1921 aagacgctgt taaagaaagt tttaaaaatc ctagagaaat tgaaatgaac ttagtcgatg
     1981 cacaacaagc gcgtcgaata ttagatagat tagttggcta taacatctcg ccagttcttt
     2041 ggaaaaaagt aaaaaaaggg ttgtcagcgg gtcgagttca atctgttgca cttcgtttag
     2101 tcattgaccg tgaaaatgaa attcgaaact ttaaaccaga agaatattgg actattgaag
     2161 gagaatttag atacaaaaaa tcaaaattca atgctaaatt ccttcattat aaaaataaac
     2221 cttttaaact aaaaacgaaa aaagatgttg agaaaattac agctgcatta gatggagatc
     2281 aattcgaaat tacaaacgtg actaaaaaag aaaaaacgcg taatccagca aacccattta
     2341 caacttctac attacaacaa gaggcggcac gtaaattaaa ctttaaagca agaaaaacaa
     2401 tgatggtcgc acaacaatta tatgaaggta tagatttgaa aaaacaaggt acgattggtt
     2461 taataacata tatgagaacc gattctacac gtatttcaga tactgccaaa gttgaagcaa
     2521 aacagtatat aactgataaa tacggtgaat cttacacttc taaacgtaaa gcatcaggga
     2581 aacaaggtga ccaagatgcc catgaggcta ttagaccttc aagtactatg cgtacgccag
     2641 atgatatgaa gtcatttttg acgaaagacc aataccgatt atacaaatta atttgggaac
     2701 gatttgttgc tagtcaaatg gctccagcaa tacttgatac agtctcatta gacataacac
     2761 aaggtgacat taaatttaga gcgaatggtc aaacaatcaa gtttaaagga tttatgacac
     2821 tttatgtaga aactaaagat gatagtgata gcgaaaagga aaataaactg cctaaattag
     2881 agcaaggtga taaagtcaca gcaactcaaa ttgaaccagc tcaacactat acacaaccac
     2941 ctccaagata tactgaggcg agattagtaa aaacactaga agaattgaaa attgggcgac
     3001 catcaactta tgcaccgaca atagatacga ttcaaaagcg taactatgtc aaattagaaa
     3061 gtaagcgttt tgttcctact gagttgggag aaatagttca tgaacaagtg aaagaatact
     3121 tcccagagat tattgatgtg gaattcacag tgaatatgga aacgttactt gataagattg
     3181 cagaaggcga cattacatgg aggaaagtaa tcgacggttt ctttagtagc tttaaacaag
     3241 atgttgaacg tgctgaagaa gagatggaaa agattgaaat caaagatgag ccagccggtg
     3301 aagactgtga aatttgtggt tctcctatgg ttataaaaat gggacgctat ggtaagttca
     3361 tggcttgctc aaacttcccg gattgtcgta atacaaaagc gatagttaag tctattggtg
     3421 ttaaatgtcc aaaatgtaat gatggtgacg tcgtagaaag aaaatctaaa aagaatcgtg
     3481 tcttttatgg atgttcgaaa tatcctgaat gcgactttat ctcttgggat aagccgattg
     3541 gaagagattg tccaaaatgt aaccaatatc ttgttgaaaa taaaaaaggc aagacaacac
     3601 aagtaatatg ttcaaattgc gattataaag aggcagcgca gaaataatat ttttatttcc
     3661 tagatacatt ttaagattgt taaatagaat cattagtgaa tcttatttta aagatagtaa
     3721 aggattaatc taaataagtg cggataatat aaacataaca acataattaa aagacataaa
     3781 tgacaataaa aggagtatag aaatgactca aactgtaaat gtaataggtg ctggtcttgc
     3841 cggttcagaa gcggcatatc aattagctga aagaggaatt aaagttaatc taatagagat
     3901 gagacctgtt aaacaaacac cagcgcacca tactgataaa tttgcggaac ttgtatgttc
     3961 caattcatta cgcggaaatg ctttaactaa tggtgtgggt gttttaaaag aagaaatgag
     4021 aagattgaat tctataatta ttgaagcggc tgataaggca cgagttccag ctggtggtgc
     4081 attagcagtt gatagacacg atttttcagg ttatattact gaaacactta aaaatcatga
     4141 aaatatcaca gttattaatg aagaaattaa tgccattcca gatggataca caattatcgc
     4201 aacaggacca cttactacag aaacccttgc gcaagaaata gtggacatta ctggtaaaga
     4261 tcaactttat ttctatgatg cggctgctcc aattattgaa aaagaatcta ttgatatgga
     4321 taaagtttac ttaaagtccc gttatgataa aggtgaagct gcatatttaa actgtcctat
     4381 gactgaggat gaatttaatc gcttttatga tgcagtatta gaagctgaag ttgcgcctgt
     4441 aaattcattt gaaaaagaaa aatatttcga gggttgtatg ccttttgaag taatggcaga
     4501 acgcggacgc aagacattac tatttggacc aatgaaacca gtaggattag aagatccaaa
     4561 gactgggaaa cgtccttatg cggtggttca attaagacaa gatgacgctg ctggtacact
     4621 ctacaatatt gttggcttcc aaacgcattt aaaatgggga gctcaaaaag aagtcattaa
     4681 attaattcca ggcttagaaa atgttgatat tgttagatat ggtgtgatgc atagaaatac
     4741 cttcattaat tcaccggacg tattaaacga gaaatatgaa ttgatttcac aaccaaacat
     4801 acagtttgcg ggacaaatga ctggtgttga aggttatgta gaaagcgcag ctagcggctt
     4861 agttgcaggt atcaatcttg cgcataaaat attaggcaag ggtgaggtag tatttccgag
     4921 agaaacaatg attggaagta tggcttacta tatttctcat gctaaaaaca ataagaattt
     4981 ccaacctatg aatgctaact tcgggttatt accatcttta gaaactagaa ttaaagataa
     5041 aaaagaacgc tatgaagcac aagctaatag agctttggat tacttagaaa atttcaaaaa
     5101 aactttataa aatagttaga aagactagat atgctattca ttcttaagtc atcaacgagt
     5161 aagtaatgac tttctaaatg gaaaatactt atcctagtct ttttaatttt ggaattgtta
     5221 cgtatttctg acaatttaga attcgcattc aaaaaatatc taaataaata acacgcaata
     5281 agttgattga tgtaacatgt aagagaatgt tttaaataaa ctttatttaa aaggcaatga
     5341 aataataaat ggcaaggcta ttaataaaga cttttagtaa ttaatttaaa aaagaggtat
     5401 tctaattaac aggttttccg attagttaca attatttaat tctcaaaaga tttagaattg
     5461 attatcaaat tactgtaagc cctttgctgt atatgctaca attcttattg atggagggta
     5521 aatgtattga atcatattca agatgcgttt ttaaatacat tgaaagttga acggaatttt
     5581 tcggaacaca cattgaaatc atatcaagat gacttaattc agtttaatca atttttagaa
     5641 caagaacatt tagagttgaa tacttttgaa tacagagatg ctagaaatta tttgagctat
     5701 ttatattcaa atcatttgaa aagaacatct gtttctcgta aaatctcaac gttaagaact
     5761 ttctatgaat attggatgac gcttgatgag aacattatta atccatttgt tcaattagta
     5821 catccgaaaa aagaaaaata tcttccgcaa ttcttttacg aagaagaaat ggaagcgtta
     5881 ttcaaaactg tagaagagga cacttcaaaa aatttacggg atcgagttat tcttgaattg
     5941 ttgtatgcta caggcatccg tgtttcggaa ttagtaaata ttaaaaaaca agatatagat
     6001 ttttacgcga atggtgttac cgtattagga aaagggagca aagagcgctt tgtaccgttt
     6061 ggtgcttatt gtagacaaag catcgaaaat tatttagaac atttcaaacc aattcagtca
     6121 tgcaatcatg attttcttat tgtaaatatg aagggtgaag caatcactga acgcggtgta
     6181 cgatatgttt taaatgatat tgttaaacga acagcaggcg taagtgagat tcatccccac
     6241 aagctcagac atacatttgc aacgcattta ttgaatcaag gtgcagacct aagaacagta
     6301 caatcgttat taggtcatgt taatttgtca acaactggta aatatacaca cgtatctaac
     6361 caacaattaa gaaaagtgta tctaaatgca catcctcgag cgaaaaagga gaatgaaaca
     6421 tgagtaatac aacattacat gcaacaacaa tttatgctgt aagacataat gggaaagcag
     6481 ctatggctgg agatgggcaa gtaacgcttg gtcaacaagt catcatgaaa caaacggcaa
     6541 gaaaagtgcg acgtttatat gaaggtaaag tgttagctgg tttcgcaggt agtgtagcag
     6601 atgcgtttac gttatttgaa aaattcgaaa caaaattaca acagtttagt ggtaacttag
     6661 aaagagctgc tgttgaattg gcacaagaat ggcgaggcga taaacaatta cgtcaattag
     6721 aagctatgct aattgtaatg gataaagatg ctattttagt tgtcagtgga actggcgaag
     6781 ttattgctcc agatgatgac cttatcgcta ttggatcagg aggcaactac gcattaagcg
     6841 caggacgtgc attgaaacgc catgcatcgc atttgtctgc tgaagaaatg gcatatgaga
     6901 gcttgaaagt agcggctgat atttgtgtct ttaccaacga taatattgtt gtcgaaacac
     6961 tataataatc agagcacgat aaataattac gagcaattaa ttttagttaa aagacggagg
     7021 aatgaaatta atggatacag ctggaataag attaactcca aaagaaatcg tatctaaatt
     7081 aaatgaatac atcgttggac aaaatgatgc taaacgtaaa gtggcaattg ccctacgtaa
     7141 tcgatacaga agaagtttat tagatgagga atcaaagcaa gaaatttcac ctaaaaatat
     7201 tttgatgatt ggaccaaccg gcgttggtaa aactgaaatt gcaagaagaa tggccaaagt
     7261 tgtcggcgcg ccatttataa aagtagaagc tactaaattt actgaggtag gttatgtagg
     7321 acgagatgtt gaaagtatgg ttagagatct tgttgatgtt tcagtaagat tagtcaaggc
     7381 gcagaaaaaa tcattggtac aagatgaagc aacagctaag gccaatgaaa aacttgttaa
     7441 gttattagtt ccaagtatga aaaagaaagc gtctcaaacg aataatcctt tagagtcact
     7501 tttcggaggt gcaattccaa atttcggaca aaataacgaa gatgaagaag aaccacctac
     7561 tgaggaaatt aaaacaaaac gttctgaaat taagagacag ctagaagaag gcaaacttga
     7621 aaaagaaaag gtaagaatta aagtcgaaca agatcctggt gctttaggta tgctaggtac
     7681 aaatcaaaat cagcaaatgc aagagatgat gaatcaatta atgcctaaaa agaaagttga
     7741 gcgagaagtt gctgttgaga cggcaaggaa aatcttagct gatagttatg cggatgaact
     7801 aattgatcaa gaaagcgcta accaagaagc gcttgaatta gcagaacaaa tgggtatcat
     7861 ctttatagat gaaatcgaca aagttgcgac gaataatcat aatagtggtc aagatgtctc
     7921 aagacaaggt gttcaaagag atattttacc tatacttgaa ggtagcgtta ttcaaaccaa
     7981 atatggtact gtgaatactg aacatatgct gtttataggt gctggagctt tccatgtatc
     8041 taagccgagt gacttgatac cagaattgca aggtcgtttt ccgattagag ttgaacttga
     8101 tagtttatcg gtagaagatt ttgtaagaat tttgacagaa ccaaaattgt cattaattaa
     8161 acaatatgaa gcattgcttc aaacagaaga agttactgta aactttaccg atgaagcaat
     8221 tactcgctta gctgagattg cttatcaagt aaatcaagat acagacaaca ttggtgcacg
     8281 tcgacttcat acaattttag aaaagatgct agaagattta tcattcgaag caccaagtat
     8341 gccgaatgca gttgtagata ttaccccaca atatgttgat gataaattaa aatcaatttc
     8401 aacaaataaa gatttaagtg catttattct ataaaaatat acaaaaggag aaaaattcat
     8461 gagcttatta tctaaaacga gagagttaaa cacgttactt caaaaacaca aaggtattgc
     8521 ggttgatttt aaagatgtag cacaaacgat tagtagcgta actgtaacaa atgtatttat
     8581 tgtatcgcgt cgaggtaaaa ttttaggatc gagtctaaat gaattattaa aaagtcaaag
     8641 aattattcaa atgttggaag aaagacatat tccaagtgaa tatacagaac gattaatgga
     8701 agttaaacaa acagaatcaa atattgatat cgacaatgta ttaacagtat tcccacctga
     8761 aaacagagaa ttattcatag atagtcgtac aactatcttc ccaattttag gtggagggga
     8821 aagattaggt acattagtac ttggtcgagt acatgatgat tttaatgaaa atgatttggt
     8881 actaggtgaa tatgctgcta cagttattgg tatggaaatc ttacgtgaga agcatagtga
     8941 agtagaaaaa gaagcgcgcg ataaagctgc tattacaatg gcaattaatt cattatctta
     9001 ttctgaaaaa gaagcgattg aacatatctt tgaagaactt ggcggtacgg aaggcctatt
     9061 aatcgcatca aaagttgcag atagagttgg tattactaga tctgtaattg taaatgcact
     9121 acgtaaatta gaaagtgctg gtgtaattga atcacgttct ttaggaatga aaggtacttt
     9181 cattaaagtt aaaaaagaaa aattcttaga tgaattagaa aaaagtaaat aagtcgatga
     9241 gtctgggaca taattcaatg tcctagtcgt gacgatgtta tcttggcggt agtcaatgaa
     9301 atgactttga tgattatgtt gaagtcattt ctttattttt gtctaatttt caatcaaatt
     9361 gatatttcac attaacttga tagaattacc tttaaattta tggaaaaatg cttgaaaaac
     9421 atttgcttta gacacggcat tatgttatat ttattttcgg ctataaagcc aatacacaca
     9481 tatcaaacgt gattgtgtaa agggtgcaat atttatatat tgtctttaca atagtttgct
     9541 atggaggtaa ttaaccaata ggaggaattt ataatggcag taatttcaat gaaacaatta
     9601 ctagaagcgg gtgttcactt cggtcaccaa acacgtcgtt ggaacccaaa aatgaaaaaa
     9661 tatatcttca ctgagagaaa tggtatttat atcatcgact tacaaaaaac agtgaaaaaa
     9721 gtagacgagg catacaactt cttgaaacaa gtttcagaag atggtggaca agtcttattc
     9781 gtaggaacta aaaaacaagc acaagaatca gttaaatctg aagcagaacg tgctggtcaa
     9841 ttctacatta accaaagatg gttaggtgga ttattaacta actataaaac gatctcaaaa
     9901 cgaatcaaac gtatttctga aattgaaaaa atggaagaag atggtttatt cgaagtatta
     9961 cctaaaaaag aagtagtaga acttaaaaaa gaatacgacc gtttaatcaa attcttaggc
    10021 ggaattcgtg atatgaaatc aatgcctcaa gcattattcg tagttgaccc acgtaaagag
    10081 cgtaatgcaa ttgctgaagc tcgtaaatta aatattccta tcgtaggtat cgttgacact
    10141 aactgtgatc ctgacgaaat tgactacgtt atcccagcaa acgacgatgc tatccgtgcg
    10201 gttaaattat taactgctaa aatggcagat gcaatcttag aaggtcaaca aggcgtttct
    10261 aatgaagaag tagctgcaga acaaaacatc gatttagatg aaaaagaaaa atcagaagaa
    10321 acagaagcaa ctgaagaata atcaactgtt gaatctgact tagatatagt ttaaatgggt
    10381 gataagatat taatgcttat cacctttttt aaaaagaaaa tcgaggcaaa ttacaaatat
    10441 tcaattagag tattggcaat cttgcctata ataatgctaa aatcataata tataaaatga
    10501 taacttattg gaggaataat gaatggcaac tatttcagca aaacttgtta aagaattacg
    10561 tgaaaaaact ggcgcgggta tgatggattg taaaaaagcg ctaactgaaa ctgatggtga
    10621 catcgataaa gcgattgact acctacgtga aaaaggtatt gctaaagcag ctaaaaaagc
    10681 agaccgtatt gcggctgaag gtttagtaca tgtagaaact aaaggtaacg acgcagttat
    10741 cgttgaaatc aactctgaaa cagactttgt tgctcgtaac gaaggtttcc aagagttagt
    10801 taaagaaatc gctaatcaag tattagatac aaaagctgaa actgttgaag ctttaatgga
    10861 aacaacttta ccaaatggta aatcagttga tgaaagaatt aaagaagcaa tttcaacaat
    10921 cggtgaaaaa ttaagtgttc gtcgttttgc tatcagaact aaaactgata acgatgcttt
    10981 cggcgcttac ttacacatgg gtggacgcat tggtgtatta acagttgttg aaggttcaac
    11041 tgacgaagaa gcagcaagag acgttgctat gcatatcgct gcaatcaacc ctaaatatgt
    11101 ttcttctgaa caagttagcg aagaagaaat caaccacgaa agagaagttt taaaacaaca
    11161 agcattaaat gaaggtaaac cagaaaacat cgttgaaaaa atggtggaag gacgtttacg
    11221 taaatactta caagaaattt gtgctgtaga tcaagacttc gttaaaaacc ctgatgtaac
    11281 agttgaagct ttcttaaaaa caaaaggtgg aaaacttgtt gacttcgtac gctatgaagt
    11341 aggcgaaggt atggaaaaac gcgaagaaaa ctttgcggat gaagttaaag gacaaatgaa
    11401 ataatctgtc ataaagtaaa acaaggaaga agacaccttt aatgttgctt tattaaaatg
    11461 taaatcattc taataaaacg acaactgtgt cttctttact tgtatatgtt acatatattc
    11521 acgatagaga ggataagaaa atggctcaaa tttctaaata taaacgtgta gttttgaaac
    11581 taagtggtga agcgttagct ggagaaaaag gatttggcat aaatccagta attattaaaa
    11641 gtgttgctga gcaagtggct gaagttgcta aaatggactg tgaaatcgca gtaatcgttg
    11701 gtggcggaaa catttggaga ggtaaaacag gtagtgactt aggtatggac cgtggaactg
    11761 ctgattacat gggtatgctt gcaactgtaa tgaatgcctt agcattacaa gatagtttag
    11821 aacaattgga ttgtgataca cgagtattaa catctattga aatgaagcaa gtggctgaac
    11881 cttatattcg tcgtcgtgca attagacact tagaaaagaa acgcgtagtt atttttgctg
    11941 caggtattgg aaacccatac ttctctacag atactacagc ggcattacgt gctgcagaag
    12001 ttgaagcaga tgttatttta atgggcaaaa ataatgtaga tggtgtatat tctgcagatc
    12061 ctaaagtaaa caaagatgcg gtaaaatatg aacatttaac gcatattcaa atgcttcaag
    12121 aaggtttaca agtaatggat tcaacagcat cctcattctg tatggataat aacattccgt
    12181 taactgtttt ctctattatg gaagaaggaa atattaaacg tgctgttatg ggtgaaaaga
    12241 taggtacgtt aattacaaaa taaatttaga ggtgtaaaat aatgagtgac attattaatg
    12301 aaactaaatc aagaatgcaa aaatcaatcg aaagcttatc acgtgaatta gctaacatca
    12361 gtgcaggaag agctaattca aatttattaa acggcgtaac agttgattac tatggtgcac
    12421 caacacctgt acaacaatta gcaagcatca atgttccaga agcacgttta cttgttattt
    12481 ctccatacga caaaacttct gtagctgaca tcgaaaaagc gataatagca gctaacttag
    12541 gtgttaaccc aacaagtgat ggtgaagtga tacgtattgc tgtacctgcc ttaacagaag
    12601 aacgtagaaa agagcgcgtt aaagatgtta agaaaattgg tgaagaagct aaagtatctg
    12661 ttcgaaatat tcgtcgtgat atgaatgatc agttgaaaaa agatgaaaaa aatggcgaca
    12721 ttactgaaga tgagttgaga agtggtactg aagatgttca gaaagcaaca gacaattcaa
    12781 taaaagaaat tgatcaaatg attgctgata aagaaaaaga tattatgtca gtataaaact
    12841 aatatacaat gacatattaa aatgccagta ttaaacgata atgtaacatt taaaatgggc
    12901 atgtttaatt aaatcaaaga tgcatgtgat aatttaaatt cacaatgagc ataaaaatgg
    12961 tgtttaaaca agttaattaa acatatactt tataaataat aggcattagg tatattgcta
    13021 taataaagtt atgtaatttt taacctcagt atgtatgtca catttctggt gtaaactgta
    13081 ccgagtcaga ctttggtaca gtttttttat ttgcttattc aatgcattaa atgagtatga
    13141 taaaatgata atgattgttt agtaacttat actatatgac agagatgatc aggctcggag
    13201 gaaagaccat gtttaaaaag ctaataaata aaaagaacac tataaataat tataatgaag
    13261 aattagactc gtctaatata cctgaacata tcgctattat tatggatggt aatgggcgat
    13321 gggctaagaa gcgaaaaatg cctagaatta aaggtcatta cgaaggtatg caaacaataa
    13381 aaaaaattac tagggtagct agtgatattg gtgttaagta cttaacttta tacgcctttt
    13441 ccactgaaaa ttggtcaaga cctgaaagtg aagtaaatta tattatgaat ttgcctgtca
    13501 atttcttaaa gacattctta ccggaactaa ttgaaaaaaa tgtcaaagtt gaaacaattg
    13561 gatttactga taagttgcca aaatcaacga tagaagcaat taataatgct aaagaaaaga
    13621 cagctaataa taccggctta aaattaatat ttgcaattaa ttatggtggc agagcagaac
    13681 ttgttcatag tattaaaaat atgtttgacg agcttcatca acaaggttta aatagtgata
    13741 tcatagatga aacatatata aacaatcatt taatgacaaa agactatcct gatccagagt
    13801 tgttaattcg tacttcagga gaacaaagaa taagtaattt cttgatttgg caagtttcgt
    13861 atagtgaatt tatctttaat caaaaattat ggcctgactt tgacgaagat gaattaatta
    13921 aatgtataaa aatttatcag tcacgtcaaa gacgctttgg cggattgagt gaggagtagt
    13981 atagtatgaa agttagaacg ctgacagcta ttattgcctt aatcgtattc ttgcctatct
    14041 tgttaaaagg cggccttgtg ttaatgatat ttgctaatat attagcattg attgcattaa
    14101 aagaattgtt gaatatgaat atgattaaat ttgtttcagt tcctggttta attagtgcag
    14161 ttggtcttat catcattatg ttgccacaac atgcagggcc atgggtacaa gtaattcaat
    14221 taaaaagttt aattgcaatg agctttattg tattaagtta tactgtctta tctaaaaaca
    14281 gatttagttt tatggatgct gcattttgct taatgtctgt ggcttatgta ggcattggtt
    14341 ttatgttctt ttatgaaacg agatcagaag gattacatta catattatat gcctttttaa
    14401 ttgtttggct tacagataca ggggcttact tgtttggtaa aatgatgggt aaacataagc
    14461 tttggccagt aataagtccg aataaaacaa tcgaaggatt cataggtggc ttgttctgta
    14521 gtttgatagt accacttgca atgttatatt ttgtagattt caatatgaat gtatggatat
    14581 tacttggagt gacattgatt ttaagtttat ttggtcaatt aggtgattta gtggaatcag
    14641 gatttaagcg tcatttcggc gttaaagact caggtcgaat actacctgga cacggtggta
    14701 ttttagaccg atttgacagc tttatgtttg tgttaccatt attaaatatt ttattaatac
    14761 aatcttaatg ctgagaacaa atcaataaac gtaaagagga gttgctgaga taatttaatg
    14821 aatctcagaa ctccttt
//
LOCUS       DALAGF010000071        14514 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.071, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000071 DALAGF010000000
VERSION     DALAGF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14514
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.071"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            197..466
                     /gene="rpsN"
                     /locus_tag="O2692_001908"
     CDS             197..466
                     /gene="rpsN"
                     /locus_tag="O2692_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCZ4299533.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            623..1599
                     /gene="guaC"
                     /locus_tag="O2692_001909"
                     /pseudo
     CDS             623..1599
                     /gene="guaC"
                     /locus_tag="O2692_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
     gene            1621..2637
                     /locus_tag="O2692_001910"
     CDS             1621..2637
                     /locus_tag="O2692_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCZ4299534.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMANNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
     gene            complement(3017..3640)
                     /gene="lexA"
                     /locus_tag="O2692_001911"
     CDS             complement(3017..3640)
                     /gene="lexA"
                     /locus_tag="O2692_001911"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCZ4299535.1"
                     /translation="MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            3783..4016
                     /locus_tag="O2692_001912"
     CDS             3783..4016
                     /locus_tag="O2692_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299536.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            4153..4392
                     /locus_tag="O2692_001913"
     CDS             4153..4392
                     /locus_tag="O2692_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCZ4299537.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            4513..6501
                     /gene="tkt"
                     /locus_tag="O2692_001914"
     CDS             4513..6501
                     /gene="tkt"
                     /locus_tag="O2692_001914"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCZ4299538.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            6779..7021
                     /locus_tag="O2692_001915"
     CDS             6779..7021
                     /locus_tag="O2692_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCZ4299539.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            7008..7106
                     /locus_tag="O2692_001916"
     CDS             7008..7106
                     /locus_tag="O2692_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299540.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            7200..7667
                     /locus_tag="O2692_001917"
     CDS             7200..7667
                     /locus_tag="O2692_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HCZ4299541.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            7791..8912
                     /locus_tag="O2692_001918"
     CDS             7791..8912
                     /locus_tag="O2692_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCZ4299542.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIIISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISE
                     KDDMSIIEMFYKHITDKELSDIQSKKIKNILENELRKED"
     gene            8916..11945
                     /locus_tag="O2692_001919"
     CDS             8916..11945
                     /locus_tag="O2692_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCZ4299543.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKEKAQIENRYQQIDLLWQEIESFDDD
                     NIKGLLEVATQQIDKLIENIPLLQARSKEILASVNESKETAIKEFEIIEKKTLENNIL
                     KDNINQLNKNKIDFVQLKEQQPEIEGIEAKLKLLQDITNLLNYIENREKIETKIANSK
                     KDISKTNNKILNLDCDKRNIDKEKKMLEENGDLIESKISFIDKTRVLFNDINKYQQSY
                     LNIERLRTEGEQLGDELNDLIKGLETVEDSIGNNQSDYEKIIELNNTITNINNEINII
                     KENEKAKAELDKLLGSKQELENQINEETSILKNLEIKLDRYDKTKLDLNDKESFISEI
                     KSAVNIGDQCPICGNEIQDLGHHIDFDSIAKRQNEIKEIEANIHAIKSNIAVHNSEIK
                     FVNEKISNINIKTQSDFSLEVLNKRLLENENALNNQRDLNKFIEQMKEEKDNLTLQIH
                     NKQLRLNKNESELKLCRDLITEFETLSKYNNITNFEVDYKKYVQDVNQHQELSKEIED
                     KLMQLSQRKLIEQNNLNHYENQLETYNNDLELNEQSIEMEMSRLNLTDDNDIDEIIAW
                     RGEQEELEQKRDTYKKRYHEFEMEIARLESLTKDKELLDSDKLKDEYELKKGKMNTLI
                     DEYSAVHYQCQNNINKTQSIVSHINYLNQELKDQQEIFQLAEIVSGKNNKNLTLENFV
                     LIYYLDQIIAQANLRLATMSDNRYQLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLS
                     GGETFQSSLALALGLSEIVQQQSGGISLESIFIDEGFGTLDQETLETALDTLLNLKST
                     GRMVGIISHVSELKNRIPLVLEVKSDQYQSSTRFKRN"
     gene            complement(12016..12378)
                     /gene="mscL"
                     /locus_tag="O2692_001920"
     CDS             complement(12016..12378)
                     /gene="mscL"
                     /locus_tag="O2692_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCZ4299544.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
     gene            12582..14228
                     /locus_tag="O2692_001921"
     CDS             12582..14228
                     /locus_tag="O2692_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCZ4299545.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT         5408 a         1925 c         2574 g         4607 t
ORIGIN      
        1 caaagcggaa tttaataagt acgaagtaat tctgggtatg ttttataaat gttcgataat
       61 acactttaat cttaaatatg atggtttaga aaatgattta acaaagaaat gaaactttac
      121 tgttgaatta tgtgaggatt gtgttattat ataaatcgta ataattacga tttgataaaa
      181 agtgaggtaa ctatatatgg ctaagaaatc taaaatagca aaagagagaa aaagagaaga
      241 gttagtaaat aaatattacg aattacgtaa agagttaaaa gcaaaaggtg attacgaagc
      301 gttaagaaaa ttaccaagag attcatcacc tacacgttta actagaagat gtaaagtaac
      361 tggaagacct agaggtgtat tacgtaaatt tgaaatgtct cgtattgcgt ttagagaaca
      421 tgcgcacaaa ggacaaattc caggtgttaa aaaatcaagt tggtaaaatc aaatttcgta
      481 ctttagccca tttacaatat caataaaaca atgtacagta tatacgaatg ctataaactg
      541 aatgttttct catattaata aggaaacatt cggtttttaa tttgcattta aaaataatta
      601 gtttttaaag gggctattta aagtgaaaat atttgattac gaagatattc aattaatacc
      661 taataaatgc atagttgaaa gtaggtctga atgtgataca actatccaat ttggtccgaa
      721 aaaattcaag ctacctgtag ttcctgcaaa tatgcaaaca gttatgaatg agaaattagc
      781 gaaatggttt gctgaaaatg attactttta tatcatgcat cgttttgatg aagaagcaag
      841 aatacctttt ataaaacata tgcaaaattc aggcttattt gcatctattt cagttggtgt
      901 aaagaaagcg gaatttgatt ttattgaaaa gttagctcaa gaaaaattaa tccccgaata
      961 tattacaata gatattgcgc atggtcactc agattcagtg ataaacatga ttaaacatat
     1021 aaaaacccat atacctgata gttttgttat tgctggtaat gttggtacgc cagaaggtgt
     1081 tagagaatta gaaaatgctg gtgctgatgc taccaaagtc ggtataggtc ctggtagagt
     1141 ttgtattaca aagattaaaa caggttttgg tactggtggt tggcagttag cggcattaaa
     1201 catatgtagt aaagcagctc gtaaaccttt gattgccgat ggtggtataa gaacgcatgg
     1261 cgacattgct aaatcaatta gatttggtgc atcaatggtc atgattggtt cattatttgc
     1321 ggcacacgaa gaatcacctg gtgaaactgt agaacttgat ggtaaacagt ataaagaata
     1381 ttttggtagt gcatctgaat ttcaaaaagg cgaacataaa aatgtagaag gtaaaaaatg
     1441 tttgtagaac ataagggttc attaatggat accttaaaag aaatgcaaca agatttacaa
     1501 agctcaattt catatgccgg tggaaaagac ttgaaatcat tacgtactgt agattatgtt
     1561 attgttagaa actctatttt caacggtgat agagattaat atttatagta ggtgatgtaa
     1621 attaaaaaat tcatagtaac tgttgttgcg tttttatcaa ttattatcat tgcgccaata
     1681 acagaattta aaccattcat tcatttacaa aatgaagtaa gacaatatat tgacattcac
     1741 atcaataaag aaacaatttc tgcggaaaat aaattggata caccgaagaa acaacaattt
     1801 gcctttaata atatacaaat gaacatgtcg aaatcagatg ttgagaaaac attaaataaa
     1861 ccaaaaagag tgacatttaa tgaatatggt acgaagtggt atacgtatta tgatgacgat
     1921 tacaataatt ttataatgat aagttacatg aaagataaag ttaatgcgtt atatacaaat
     1981 caaaatataa tcacttcaaa atcaaaaatt aaatataata cacctaaatc ggttgtaagg
     2041 caaagattag gcgaaccaga aacagagatt gttaaaggta gagtgcgtta cgaacaaaat
     2101 aataaagaat atgatgtttt ccataaaaat cacatttata cgacggtatt ttatgataag
     2161 catcgacgta ataatgtaac agctgtttta caagtaagtg atgctatgga aaatagatta
     2221 aaagaacaat atggagcacc atcgaaatcg cttgcagata gttttgaact acaaaatttt
     2281 gatttagtta atgctgaaag aaaacaacat caattatcta cattgaagta ttctaaacag
     2341 aattctgaaa ctgcacgtaa gcatagtaaa gatatggcca acaatcatta ttttgatcat
     2401 acaaatttaa aaggtcaatc accatttgat cgattgaaaa aagatggtat tacatttaac
     2461 tcagccggag agaatttagc atatggtcaa gttagtagta tctatgcaca tcaaggatta
     2521 atgaattcta ttggtcacag aaaaaatatt ttaaatgata cgtttaaaat attaggtgtt
     2581 ggtgttgatt ttaatgatga aaaacaacct ttttggacag aaaattatac tggttaatgt
     2641 ttttggcatg agattaaagg gtaatgtttg tctgtaagga agtagataaa catgatgaca
     2701 acagataaac caaaagatgc ggatatactt gaacgcgtca aagatatact taataaaaaa
     2761 gaaagaaaaa aataataatg gctcgctcct gtaaattatt acgggggcga gttttaattt
     2821 gtgtaacatt ttgcagaaat ctgtaattca aaatttatat taaagaatga gattatctaa
     2881 caattcgcaa tttctgtttg aactgaattt agattagaat aaataaaacc tcttgaaaac
     2941 gtaacttttt caagaggtcc aaaatttata aaaatgaggg cttaacaatt acgataatat
     3001 ataacggttt aaaatattac atttcgcggt acaaaccaat tactttccca attacagcaa
     3061 cattgtctag gtaaattggc tccattgtac tattttcagg ttgtaatcga taacgatttt
     3121 tttctttata gaagcgtttg acagttgctt catcttcctc agtcatagca acaataatgt
     3181 ctccattttc tgctatggtt tgactgcgaa caattacttt gtctccgtct aatataccag
     3241 cctcaatcat actgtcgcct acgacgttta atatgaatat gtcgctattg tgtgtcgatg
     3301 ttaagtgttc aggtaatgga aaatattctt caatattttc tactgcggta ataggaacac
     3361 ctgctgtgac tttaccaata actggcacat gaatcgtttc ttccatatta atattatcat
     3421 ttgtttgatc acttacaatt tctatagcac gtggtttcgt tggatctctt cttatatagc
     3481 ctttttcttc aagacgtgaa aggtgaccat gaacagttga actggatgct aagccaactg
     3541 cttcaccaat ttcgcgaaca ctaggcggat aacctttcgt ttgaacaact tgtttaatat
     3601 agttatatat ttcgctttgt cgttttgtta attctctcat atataggcac tccctaaatt
     3661 aatttaaata cagtatatca ttaatttgaa accaaaacaa acatttgttc gttaaaatat
     3721 tgacacagaa cataagttct gataaattac taatacaaac aaatgttcta ggagtgaaaa
     3781 tgatgtttta caataaatat aaaaacgtat caacatatat catcatattt ttagtttcaa
     3841 gtgcagcctt tgcaatattc ttgttaagtg cgaacattag tgctcactcg gaacaagtgt
     3901 acgaaatgac tgaccatcaa attaagaaca atacgataaa taaagcatac gaacataaag
     3961 accctacaaa caatagcgaa caaagagatg ggaaagtgtt cgctttaata aattgataca
     4021 ttgtcacaac gttattttgc ctatttttgc gaaatagcgt tttttattac tttttttgct
     4081 gatcttaaat ttgttatatt ttgttaaagt attataatga ttgaataaac aaattgaagg
     4141 taggtttttt aattgagtaa ttctgatttg aatatcgaaa gaattaacga gttagctaaa
     4201 aagaaaaaag aagtaggatt aactcaagaa gaagcaaagg agcaaacagc cttaagaaaa
     4261 gcttatcttg agagttttag aaaagggttt aaacaacaaa ttgaaaatac taaagtaatt
     4321 gatccagaag gtaatgatgt aacacctgaa aaaattaaag agatacaaca aaaaagagat
     4381 aataaaaatt aaatcacaaa tctgtaaaga attttctgac attataactt gaaataagta
     4441 ttttacttat ctttttattt taaaataagt tataatgtat ttgataaaat tgaagaaggg
     4501 aagatacaca agatgtttaa tgaaaaagat caattagctg ttgatacgct acgtgcacta
     4561 agtatcgaca caatcgaaaa agcgaattct ggtcatccag gattacctat gggagctgcc
     4621 ccaatggctt acactttgtg gacacgtcat ctgaatttta atccacaatc taaagattac
     4681 ttcaatagag accgtttcgt attatctgca gggcatggtt cagcattatt gtatagcttg
     4741 ttacatgttt ctggtagttt agaattagaa gaattaaagc aatttagaca atggggttct
     4801 aaaacaccag gtcatcctga atacagacat acagatggtg tagaagttac taccggacca
     4861 cttggacaag gttttgctat gtcagtagga ttagctttag cagaagatca cctagcaggg
     4921 aaatttaata aagaaggata taatgttgta gatcattaca catatgtatt agcttctgac
     4981 ggtgatttaa tggaaggtat atcgcatgaa gcagcttcat ttgctggaca taataaatta
     5041 agtaaattag ttgttttata cgattcaaat gatatttcat tagatggcga attaaacaaa
     5101 gctttttctg aaaacacaaa agctcgtttt gaagcatatg gttggaatta cttactagtt
     5161 aaagatggta atgatttaga agaaattgat aaagcgatta ctacagctaa atctcaagaa
     5221 ggaccaacga ttattgaagt taaaacaaca atcggatttg gttcaccgaa taaagcagga
     5281 actaatggtg ttcatggggc acctttaggt gaagttgaaa gaaaattaac attcgaaaat
     5341 tacggtttag atcctgaaaa acgttttaat gtttcagaag aggtatacga aattttccaa
     5401 aatactatgt taaaacgtgc taatgaagat gaatctcaat ggaattcatt attagaaaaa
     5461 tatgcagaaa catatcctga attagcagaa gaatttaaat tagcgattag tggtaaattg
     5521 cctaaaaatt ataaggatga attaccacgt tttgaactgg gtcataatgg tgcatctcgt
     5581 gctgattctg gtactgttat tcaagcaatc agtaaaactg tcccttcatt ctttggtgga
     5641 tcagcagacc ttgctggttc aaacaaatcc aatgtaaatg atgcaactga ttatagttct
     5701 gaaacacctg aaggtaaaaa tgtgtggttt ggtgtacgtg aatttgctat gggtgctgct
     5761 gtaaatggta tggctgcaca tggaggttta catccatatg gtgcaacatt cttcgtattt
     5821 agtgattatt taaaaccagc gttacgttta tcatcaatta tgggattaaa tgcaacgttc
     5881 atcttcacac atgattcaat tgcagtaggt gaagatggtc ctactcatga accaattgag
     5941 caattagctg gattaagagc cattccaaat atgaatgtta tccgtcctgc tgatggtaat
     6001 gaaacaagag tagcatggga agttgcctta gaatctgaat ctacacctac ttcattagta
     6061 ttgacacgtc aaaacttacc ggtattagat gtaccagaag atgtagttga agaaggcgtt
     6121 cgaaaaggtg cctatacagt ttatggctct gaagagacac cagaattcct attattagct
     6181 tcaggttcag aagttagtct tgcagttgaa gctgctaaag atcttgaaaa acaaggtaaa
     6241 tcagtacgtg ttgtttcaat gcctaactgg aatgcatttg aacaacaatc tgaagaatat
     6301 aaagaatcag ttattccatc aagcgtaaca aaacgtgttg cgattgaaat ggcttcaccg
     6361 cttggatggc ataaatatgt aggtactgca ggtaaagtta ttgctattga cggctttggc
     6421 gcaagtgcac ctggcgattt agtagttgaa aaatatggat ttacaaaaga aaatatctta
     6481 aaccaagtta tgagcttata agaataattt ataaagcgag tatgtttaga agtctaggat
     6541 gcataatctt aggcttcttt taaagtgttg aaatttagag tatagcactt aaactacatc
     6601 ataagtgata agttatgaaa gtatactatt tcagattaat ctttaaaagc tctgttataa
     6661 cagcatgatt tttgatatta tttttagtat cgatattaaa atacttgaat aaactagttc
     6721 ttgaaataat gtgatgaatt tagtaaaatt cagtaggata aagaaagcgg gtgaaacaat
     6781 ggcaacttgg ttagcaatta tttttatagt agctgcatta attttaggtt taattggagg
     6841 tttcctttta gctagaaaat atatgatgga ctacttgaag aaaaacccac caatcaacga
     6901 agaaatgctt cgtatgatga tgatgcaaat gggtcaaaaa ccttctcaga agaaaattaa
     6961 tcaaatgatg acgatgatga ataaaaatat ggatcaaaat atgaagagtg cgaaaaagta
     7021 aattcgcaat tgatagaggc tattttccag atatggaaat ggcctctttt tataatcaaa
     7081 ttaataagaa taaatatgtt tattaaaatt aaagttaaca aaatgacgaa tagactgaga
     7141 aatgctataa ttcattttgt atgatttaca gagagtttat ttaacgagaa ggtgtctgcg
     7201 tgctctattt aatattttca atcattgtag ctttatttat gggaactata gttatagtta
     7261 ttcgtatgaa agctcaaaat tatccggtaa atgagaaaaa aatagttttg ccaccgtttt
     7321 ttatggcgac cggtgcattg atgtacgtcg ttccatattt taggctaaca ggatcggaaa
     7381 tgctagaagc ctttataatt ggtttgcttt tttctacagt tctaatttgg acttctcgat
     7441 ttgaagtcaa aggtacagaa atttatatga aacgatctaa agcatttcca gttattttga
     7501 tttcattact tatcattcgt actgtgatga aaatattcat tagtaatgaa atagatcctg
     7561 gagaattagg cggcatgttc tttttattag cattctgtat gattgttcct tggagagcag
     7621 caatgctata taaatacaaa aaactaaaga aaacattaat caattaatta cttttaaaac
     7681 cacttgtgat cgacttctaa atcagtcaat gagtggtttt aattttactt gaaaaggtga
     7741 aatgattata ttaaaataaa agcgaacaaa tgttctatat ggaggcagta atgaaaatta
     7801 tacatacagc agactggcac ttagggaaaa tattaaatgg caaacagctt ttagaagatc
     7861 aagcgtatat tttagatatg ttcgtagaaa aaatgaaaga agaagaacct gatatcattg
     7921 tgatagctgg agatttatat gacacaacat atccaagtaa agatgcaatc atgttattag
     7981 aacaagcgat tggaaagtta aatttagaac tgcgtatacc aataattatt attagtggaa
     8041 atcacgatgg taaagagagg ttaaactatg gggcgagttg gtttgaacat aatcagttat
     8101 ttataagaac agattttaca tcgattaatt caccaataga gataaatggg gttaattttt
     8161 atacactccc ttatgctact gtgagcgaaa tgaaacacta ctttgaagat gacaccattg
     8221 aaacacatca acagggaatt acgcgctgta ttgaaacaat agcaccggaa attgatgaag
     8281 atgccgtcaa tattttaatt agtcatctga ctgttcaagg tggaaagaca tctgattctg
     8341 aaagaccatt aactattgga acggttgaat cagttcagaa aggtgttttt gatatatttg
     8401 attatgtcat gctaggtcac ttgcatcatc catttagtat agaagacgac aaaattaaat
     8461 atagtggctc cttattgcag tattcatttt cggaagcggg tcaagctaaa gggtatagac
     8521 gtgtaacaat taatgatggc attattaacg atgtatttat tcctcttaag ccacttagac
     8581 aattggaaat tatctcaggc gaatataatg atgttattaa tgaaaaagtt catgtgaaaa
     8641 ataaagataa ttatttacat tttaaactta aaaatatgtc tcatattact gatccaatga
     8701 tgagtttaaa acaaatttat cctaatactt tagcgctgac gaatgaaact tttaattaca
     8761 atgaagaaaa taatgctata gaaataagtg aaaaagatga catgtcaatt atcgaaatgt
     8821 tttataaaca tataactgat aaagaattat cggatatcca atctaaaaag ataaaaaata
     8881 ttttagaaaa cgaattgaga aaggaggatt aatgaatgaa accattacat ttaaagttga
     8941 ataatttcgg ccccttttta aaagaagaaa ttgatttttc taaaattgat aataatgaat
     9001 tgtttttaat aagtggtaag actggatcgg gtaaaacaat gatttttgat gcaatgactt
     9061 atgccttgtt tggtaaagca tcaactgaac aaagagaaga aaatgatttg agaagtcatt
     9121 tcgctgatgg taaacagccg atgtcagtaa catttgaatt tcaattaaat catcgaattt
     9181 ataaagtgca tagacaaggc ccttatatca aagaaggtaa tacaacaaaa acgaacgcta
     9241 aatttgatgt atttgagatg gtggatggca agtatgaaat tagagaaagt aaagtaattt
     9301 caggtaccca attcattatt gaattattag gagtaaatgc agatcaattc cgacaattgt
     9361 ttattttgcc tcaaggtgaa ttcaaacgct ttttaatatc aaacagtcgt gaaaagcaag
     9421 ggatattaag aacactgttt gacagtgaaa aatttgaagc tatacgagaa atattaaaag
     9481 aagaagtaaa aaaagaaaaa gctcaaatcg agaatagata tcaacaaatt gaccttttat
     9541 ggcaagaaat tgaatcattt gatgatgaca atataaaagg cttattagag gttgccactc
     9601 aacagataga caaattgatt gaaaatatac cacttttaca agctaggtcg aaagaaatac
     9661 tagcatctgt aaatgaaagt aaagaaactg ctattaaaga atttgaaata atagaaaaga
     9721 aaacattaga aaataatata ttaaaagata atattaatca actcaacaaa aataaaattg
     9781 atttcgttca attgaaagaa caacaacctg aaatagaggg aattgaagct aagttaaagt
     9841 tgttacaaga tattacaaac ctattgaatt atattgaaaa tagagaaaaa attgaaacta
     9901 aaattgctaa tagcaaaaaa gatatttcta aaaccaataa taaaatattg aatcttgatt
     9961 gtgataagcg aaacatagac aaagagaaaa aaatgttaga agaaaatgga gatttaattg
    10021 aaagtaaaat ctcttttatt gataaaacta gagtattatt taacgatatt aataagtatc
    10081 aacaaagtta tctcaatatt gaacgcttga gaactgaggg tgaacaatta ggtgatgaat
    10141 taaatgatct aattaaaggt ttagaaacgg tcgaagattc aataggtaat aaccaaagtg
    10201 attacgagaa aattatcgaa ctaaataata cgataacgaa cataaataat gaaattaata
    10261 taattaaaga aaatgaaaaa gctaaagctg aattagataa actattaggt agtaagcaag
    10321 agttagagaa tcaaattaat gaagaaacat ctatattgaa gaatctcgaa ataaaattag
    10381 atcgctacga taaaacaaaa ttggacttaa atgataaaga aagctttata agtgaaatta
    10441 aatctgctgt aaatattgga gatcaatgtc cgatatgtgg taatgaaatt caggatttag
    10501 ggcatcatat tgattttgac agtattgcta aacgtcaaaa tgaaattaaa gaaattgaag
    10561 caaatatcca cgctataaaa tcgaatattg ctgtgcataa ttctgaaatt aaatttgtta
    10621 atgaaaaaat atcgaatatt aatattaaaa cgcaaagtga tttttcactt gaagtattga
    10681 ataagcgtct gctagaaaat gaaaatgcat tgaataatca aagagatctt aataaattta
    10741 tagaacaaat gaaagaagaa aaagataatc taacgttgca aattcataat aaacaattgc
    10801 gtctaaataa aaatgaatct gagttgaaat tatgtcgaga tctcatcact gaatttgaaa
    10861 cactctcgaa atataataat atcactaatt ttgaggtgga ttataagaag tatgttcaag
    10921 atgtgaatca gcatcaagaa ctctcaaagg agattgaaga taagttaatg caattgtctc
    10981 aaagaaagtt aattgagcaa aataatctaa atcattatga aaatcaacta gaaacttaca
    11041 ataatgactt agaattgaat gaacagtcta ttgaaatgga aatgtcgagg ctgaatttaa
    11101 ctgatgacaa tgatatagat gaaataatag cctggagggg cgagcaagag gaattagagc
    11161 agaaaaggga tacttataaa aaacgttatc atgaatttga aatggaaata gctaggttag
    11221 aatcattaac taaggataaa gagttattgg actctgataa attaaaagat gaatatgagc
    11281 taaaaaaagg aaagatgaat acactgatag atgaatactc tgctgttcat tatcaatgtc
    11341 aaaataatat taataaaaca caatctatag tttcgcatat taattactta aatcaagaat
    11401 taaaggatca acaagaaata tttcaattgg ctgaaattgt cagtggtaag aataacaaaa
    11461 atcttacatt ggaaaacttt gtcttaattt actatttaga tcaaattatt gctcaagcaa
    11521 atctgagatt agcaacaatg tcagataatc gataccaact aattaggcga gaagcggttt
    11581 ctcatggtct tagtggccta gaaattgatg tatttgattt gcattcaaat aagtctagac
    11641 atattagctc gttatcaggt ggagaaactt tccaatcgtc gcttgcatta gctttagggt
    11701 taagcgaaat tgtacagcag caatcaggag gtatttcact agaatcaata tttattgatg
    11761 aaggattcgg tacattagat caagaaacgc ttgaaacagc gttagacact ttattaaatc
    11821 ttaaatcaac tggtagaatg gttgggatta tttcacatgt gagcgaattg aaaaatagaa
    11881 tacctttagt tttagaagtg aaatcagatc aatatcagag ttcaacaaga ttcaaaagaa
    11941 attaaaacat aaaaaataga gatggtactt tggaatgtat agaacaacct tagcccatct
    12001 ctttttattt aaaaattatt ttttctcacg taataaatct ctgatttcag ttaataacac
    12061 aacattttct tccacaactg cttcttcttc ggcttcttct ttcttcatta atgtatttgc
    12121 aatcttaaca aagatgaata aagcaaacgc gatgataata aagtcgataa cagattggat
    12181 aaataaaccg tatttaatac cccagaatga ccattcttta gcaaaatcaa ctgatccgaa
    12241 aattttacca attaatggca taatgatatt ttctactaat gaagatataa tcttgttgaa
    12301 agctgcaccc atcacaacag caattgctaa atctaagacg ttacctttta aggcgaactc
    12361 tttgaattct tttaacatta cactcaacct ctctttttta aatgtaacct caattataca
    12421 acaaataaaa aaattagaaa atagattatt caagaaaatt tgattatcgc aaagaccttt
    12481 gttatcctat aaaggtgcgt gagttattga ataaaatgtt tagataatat ttttattcta
    12541 ctacataaaa ttaaatatag aattgaaggg agtaattaat tatgaattct tcttcaccag
    12601 agaatccaaa tggaaagaag tattcaccag tcttcatcta tagtgcaatt gttgttgcta
    12661 tagtcgtatt acttggtgca tttttacctg aacaattcaa ctatgttacc aataatatta
    12721 aaatgtggat tacagaaaag ttaggttggt attatcttat tcttactacg attatcgtgt
    12781 tcttctgtat attccttatt tttagtccta ttggaaaact taaactaggt aaaccaaatg
    12841 acaaacctga gtttaataca atttcatggt ttgctatgtt gtttagtgct ggtatgggga
    12901 taggtttggt gttttatggt gcagctgaac cgatggcgca ctttgctacg ccacctacag
    12961 cagatcccaa aactactgaa gcttatactg aagctctacg ttcaacattt ttccattggg
    13021 gattccatgc ttgggctgtt tatggtgttg ttgcgttagc gttggcatat tcgcaattcc
    13081 gtaaaggtga accaggttta ttatctagaa ctttacgtcc tcttttaggt gataaagtag
    13141 aaggtcctat tgggattttt attgacgttt tatctgtatt tgcgacaatc gttggggtag
    13201 ccgtttcgtt aggtatgggt gctctacaaa ttaatggtgg tttacattac ttgttcaatg
    13261 ttccaaacaa tacgtttgta caagcgatta tcatcattgt tgttactatc ttatttatag
    13321 caagtgcatg gtctggatta agtaaaggta ttcaatactt aagtaacttg aacattggtt
    13381 taggtactat tttaatggta gctgctttaa ttgttggacc aactgttctt attttaaata
    13441 tgttaactag ctctacgggt agtttactaa acacattctt gtttaatagt tttgatacag
    13501 cagctttaaa tcctcaaaaa cgtgaatgga tgtcttcatg gacactttat tactggggtt
    13561 ggtggttaag ttggagtcca ttcgttggag tgtttattgc acgagtttca aaaggacgtt
    13621 caattagaga gttcatttct ggtgtcttgc tagttccagc aattgttagt tttgtttggt
    13681 ttagtgtctt tggtgtatta ggcatcgaga caggtaagaa acacaaagaa atttttgata
    13741 tgactcctga aacacagcta tttggagtgt ttaatcatgt gccatttggc attgttttat
    13801 cgttgattgc attattatta attgcatcat tctttattac atctgctgac tcagcaacat
    13861 ttgtattagg aatgcaaaca acatttggtt cattaaatcc atctagtatg gtaaaagttg
    13921 tttggggaat ttcacaggcc ttaatagcat ttgtactttt attagctggt ggcggtaacg
    13981 gcgctgaagc tttaaatgcg attcaaagtg ctgcaattat aagtgcattc ccattctcct
    14041 ttgtcgtcat actcatgatg gtaagtttct acaaggatgc gaaccaggaa cgtaaattcc
    14101 taggtttaac attgactccg aataaacatc gcttacaaga atatatcaag agtcaacaag
    14161 aagattatga atctgacatt cttgaaaagc gtcagtcacg tagaaatata gagaaaaaag
    14221 ataactaata aagtttagtt aagtatttta ataacaagta gtatgtcatt ctagtagcta
    14281 gaacagatat actacttgtt tgtttttgtg gaaaattgag tatattcaaa aggataagaa
    14341 cgagaatttc gttattaaac tacgaattct cgattttttt atattttaag ataggtttat
    14401 ttctgaaaac ttaatagaaa ggggtttgac aaagctaaag tgaagtttga cggtataaac
    14461 gcaaattaaa tatactttta tagaaaatta actcaggctg ggacataaat caat
//
LOCUS       DALAGF010000072        14192 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.072, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000072 DALAGF010000000
VERSION     DALAGF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14192
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.072"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..186
                     /locus_tag="O2692_001922"
     CDS             <1..186
                     /locus_tag="O2692_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299546.1"
                     /translation="KMYNFAYFRILEVCEMAKKNDYRFTYKDYKFLKQYGVSNTFICK
                     MYHISIEDLEFFEVMNR"
     gene            337..657
                     /locus_tag="O2692_001923"
     CDS             337..657
                     /locus_tag="O2692_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HCZ4299547.1"
                     /translation="MKPVLDIKKTLGQLNFLGVDEKYKYENGERTDKTVYAYKLASQE
                     QGEQITVKTPNKVELDYLQECELVEPDVKMYVQMSGDFGTIAYSWNAEDIKVVGSNSA
                     LKQK"
     gene            671..973
                     /locus_tag="O2692_001924"
     CDS             671..973
                     /locus_tag="O2692_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299548.1"
                     /translation="MDYYTADRLYRYTNSSNLSEPILNYVASRINWGDKVSLMTLAKE
                     IQSKFNDSYVKENTVKGRPKIYADLCLLCMSLSEAGHGRMLQVNLEDCIYIGDIDV"
     gene            1148..2257
                     /locus_tag="O2692_001925"
     CDS             1148..2257
                     /locus_tag="O2692_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCZ4299549.1"
                     /translation="MTLKNQCTPLTNRGVERTNKSAVEAVVDWVQVTFHIAPISAVIE
                     DVIGLPITLFKKRNSGIYFYNRGYEFSNIKLYYSSDDESMGIHLQLTGTGCREFEHHL
                     QQLNKTWQDFFDKCLSVNANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSA
                     INGFNLSDGRSKGATFYIGSKQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMR
                     KAYAVNCAKVLSRTDNISEIVKSILHNNLRFISPPKGGNDKNRKRWPLYRPWALFIKD
                     TEKLNLTTRPTLKSIEDNLDWLCKQVATTLDTVLTAESMAQSEGLLTDTDFLDKILAH
                     SQFNDEHTNRINHYLEALKQKSTYQKISVKKITGM"
     gene            2299..3354
                     /locus_tag="O2692_001926"
     CDS             2299..3354
                     /locus_tag="O2692_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCZ4299550.1"
                     /translation="MKIISKYILTRFSKNGKHFDIPKEQRRVIPKFKNRRKMIVICFY
                     SLLAILLILLLASFIKATRANNDSKEAVNKTNMIQKKYEDNANTVQYSPKLKLYADKF
                     IDTYMNISKDSKELESREKELLKYFPSDYKKPDEKISDTERKLNSKEFYNIKRKDKQT
                     IIQYIVNYDVNITEKKEVKVKKKKKSEKDKDEYETKTEEKQRKVNQNILINIPIKSEN
                     NKYVVVEYPYFTPIPDSQLNQAKMVEDNLKDNKREDNPKAKAFIEDFFNKYASSKSDD
                     MAYLMDNPEGLEGTREVSQIREIRLYPKGDDYVAKVEILMKDKDSPLENLEHYTLDIT
                     KKDGKYYVKNMTNSIGG"
     gene            3359..3619
                     /locus_tag="O2692_001927"
     CDS             3359..3619
                     /locus_tag="O2692_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299551.1"
                     /translation="MSGLFNFFVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKW
                     ASGKYGHMVILFVVGGFLFLVSKGPEQVFNAIAGVWKMIFGG"
     gene            3631..4014
                     /locus_tag="O2692_001928"
     CDS             3631..4014
                     /locus_tag="O2692_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCZ4299552.1"
                     /translation="MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWF
                     ILFLLFWYLLQPVIMSIGGLMFIYFTVAPYYITKYIVKLKQDGKKLFFFLWDFVIFVF
                     NVQLRKVKLSYDEEVEYHDKKITFK"
     gene            4049..6544
                     /locus_tag="O2692_001929"
     CDS             4049..6544
                     /locus_tag="O2692_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCZ4299553.1"
                     /translation="MNLKAPYAGLRDNLLLTKTGEVWAYYRVRSESISTANYDAKEES
                     KKRMRYFLESIKGYQDFHFEMYDHDLDLRNKFKEISKDFDDEAFNVAEYYSKETIDVL
                     EQELQMITHNSFVMGVKIRELADDAVTLKEILKSTLSDTAERIVSNFGFDVNLDDKIL
                     DKYKTFERDLADSFLGIGGERLTENELAYIIRKPFISNATHDVQEESSRRAITDIYNA
                     VIDPTRLSVLEVENDNEKFYTTTVVVDDFPLDMEYTHLFEKAQNMPFPVECHVKAKII
                     NNEKVKKKFDRAKINHKQQAKEKQDTGDSASEDVQDNLFVLNQMEREVTGSGVFIEWV
                     FMFVIKSDDYRDCKRKAKRLMKRLKETQIYAVNPLADQLQLFYQCLHGNDLFINKYWL
                     QRTTATGIAENLFGVSQSLGTKTGFYIGRIDKFIRSVSREEAVASSRDIILFSLLLAA
                     KGIKGAVSDSPHVLITGQTGKGKSFLAKLLWIYTSFFKGQMLYWDPKSEFAEWFDRVT
                     ESKEMQEKYPLFINHLKTFKYVTLNYEDSTNYGCLDPIVFLDGIEANEMLKSVFDEIK
                     SFENYHHIETAIYQAISDTVEERENGKKVGSLNVIEKLQNNEDEHVKNAGDLLFEKTQ
                     NNILKLVFSDGTNPALNIQEKATILQVKGLDMPKADDDTSSYSTSEKNGITLMLLIGK
                     FLEKFGSRRDVQTTIFIDEGWAFSASRQGKKVGKRIKRTGRSENNSLVFITQSVKDKA
                     DDDGGNFGCHFAFDEKDEREDILKSLGLEYSKESPENMEMLKDLKKGQCIFSDFYGRV
                     GKMVVHCPFEEMTEAFRTQEDSASSKAEEKFAM"
     gene            6598..7956
                     /locus_tag="O2692_001930"
     CDS             6598..7956
                     /locus_tag="O2692_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCZ4299554.1"
                     /translation="MAMNEIALFKGVRIQPYQKYIDFMFASVLAFIFLVYRIYILFID
                     YKSVNKKLDILTLFAYFKPHLLYLLIGTAIIFVICKLIAQFLIRFKTKDLAEMIETQG
                     FHNRTVIKKTTEYIPIDYKQTEYDYYPTMFYKRGKKSFVIHIKKDGSRFQDNYLELET
                     IIEPMFNSELVEKKHIGRYLRYEFMPLKYKKRIVMNGEQESNTIFYDTKIAITHQITW
                     DFVKAPHGLVTGITGGGKTYFLFYVIRELFRRHSEVRLLDPKVSDLSFMKRVIGDDKV
                     ADTKGQILKQLREANNEMEERFRLMNDSSDYKIGNDFRNFDMRPYFIIFDEVTAFTST
                     LDKKELQEMNDYLINIIMKGRQAGVFMFLTAQRPDADVIKGNVRDQLGLRVSLGNLSN
                     DGYRMTFGQTDKEFQTIHDSDIGRGYISILGQYNEPILFDAPLMEQYDFVEDVKQILN
                     KE"
     gene            7961..9808
                     /locus_tag="O2692_001931"
     CDS             7961..9808
                     /locus_tag="O2692_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299555.1"
                     /translation="MKHKSVYLLCFLILPILLLTSVSAYAVSNPVGEPSHAVKPKIEK
                     YDLSHYRSIYSEKGDWNPFGQEEISRQINNVSDFFFSMTKILAGVTDYAIENLFQLDV
                     INDFADKIGDFVGDIYQKLLSNLALTLFIIVCFNAFIIFSVQGNAREALKRAFLIMCL
                     IGFGVGILANAGNIIRGTNNIGKDLNNIIMNSTSSIDGNIDYIHENSGMNHIRNQLFD
                     MTIYRTYLVMNYGTVDEKEIKAKGKDRIDNILKLDYDKKSEKELDDIVKDEVEKKNNK
                     YMTQGYVFQKLAISVIGFIITLFMSIVFLSISFAKLIFSTFALFLFLFLVFSWIVSFI
                     PGFELSVFSAFAKTLGYIILSACMTFLFVIVGLCIKLANSFIEPDSQNAYFLNSIFII
                     VILFVMYKKRAQIINFVSRGNISFSPSAIGAGVMNRTQERFNKIRHEQAQNKKAKREN
                     QRDEPAPPLQNDNDLRRRQQDKPMPLFINKDNQKNGNKRREQQESMNGNDVKSASVES
                     NANNYSKQPQKASQQEHQVRETRQRKDIQRSPQVVNQPLNNENHSINRKEQKSVQTAY
                     DTDVQKRQIQNATQNQQSRQSGNRNQPITRNSQSKDRLKEQKDINKHGK"
     gene            9798..10844
                     /locus_tag="O2692_001932"
     CDS             9798..10844
                     /locus_tag="O2692_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ4299556.1"
                     /translation="MVNKKDIAKKVIGKTPIGVKLKLAKVIIILCVVAFFIFPVIIMF
                     LLAPDLNKGKDDAGCTVSGGNVSANGIDKFNENAKGGKLEGKGKEIQKIAEKNKVPVN
                     IFMAIIASESQWGKGENATRQNNPLSVMGSKSIHDSTYPTIEDGLNAGAKNLYDVYIS
                     KGLDTPKKIGPKYAPVGASNDPNNMNARWIPTVEKIMKDLGGSEAKTSCSNGKGKSIK
                     FNGKLPHWSNDDPGKGNLYTAGQCTWYAYGMRQKMGKPVSTYWHDAHKWNDRAKAEGY
                     KVDKNPEPGALFIAEQGAGGHDGHYGHVAVVIGVSDGGKTFRITEMNWEGAFKVNERT
                     LKMTDGYSFIHDKE"
     gene            10851..11441
                     /locus_tag="O2692_001933"
     CDS             10851..11441
                     /locus_tag="O2692_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299557.1"
                     /translation="MKQKIIMFTLVTVILFCAVLIGYQIPKQQVKMKQNQIEDLQEEQ
                     RILRDKNGELNKLVKRQSKTVISDEEKQIREVSSNFVKQMFEMKKDSSFKSKAPQIKP
                     LVTKDYYDTLFKDSKDKYDLYDDITVNDIHVYFDTYDPKKDSYKVFVQFDERIETDGD
                     DKIEHRQTSAQLDLVRTAEGWRIDNLKRFNLKPLGR"
     gene            11497..11853
                     /locus_tag="O2692_001934"
     CDS             11497..11853
                     /locus_tag="O2692_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin-like fold lipoprotein"
                     /protein_id="HCZ4299558.1"
                     /translation="MRRWFVLILGLVILLSACGQKYDKEIDEVTKLEKESIQDVKNTK
                     KYKNVERSKSYYKIYNDGEVIIMTYMPFKDSNTKVSRVYKINQTSDKYEEDSNIDAEK
                     FEKDNKPVYEENNMKK"
     gene            11962..12465
                     /locus_tag="O2692_001935"
     CDS             11962..12465
                     /locus_tag="O2692_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ4299559.1"
                     /translation="MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYR
                     EVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPRQI
                     ASQIEKDTGYYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGSRVI
                     KYRLKKY"
     gene            12446..12982
                     /locus_tag="O2692_001936"
     CDS             12446..12982
                     /locus_tag="O2692_001936"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCZ4299560.1"
                     /translation="MGSKSINNREEFGHYEIDTVWSVRPSTYCLLTIIERKTRYLYAS
                     RLNTRKSNVVCNEIINIMKPLLPKSITMDRGKEFALFEEIEDELNCSIYFSDLGCPYQ
                     RGSIENANGLLRQYYPKGTDFANITQKSLDEAVKQINARPRMIFDYKSSEEMLKYHVS
                     TQNCEPILNDCVRHEPVN"
     gene            13202..13750
                     /locus_tag="O2692_001937"
                     /pseudo
     CDS             13202..13750
                     /locus_tag="O2692_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
BASE COUNT         5170 a         1602 c         2663 g         4757 t
ORIGIN      
        1 aaaatgtata attttgcata ttttcgtata ttagaagttt gtgaaatggc aaaaaagaat
       61 gattatcgat ttacttataa ggattataaa tttttaaaac aatatggtgt gtctaataca
      121 tttatttgta agatgtatca tatttcaatt gaagacttag aattttttga ggtgatgaac
      181 cgataggttt gattaaaaat atgataggtc aggagaagca tagacactcc attatcaaaa
      241 atgcagtggt acggatttgc gggagttatt cctattcgtg cgacccttta gaagtcgttt
      301 aattttatta aaaattttgt aaaggatgga tatattatga aaccagtatt ggatattaaa
      361 aaaactttag gacaattaaa ctttttaggt gtagatgaaa aatataaata tgaaaatgga
      421 gagcgtactg ataaaactgt ttatgcatat aaattagcga gtcaagaaca aggcgaacaa
      481 atcactgtta aaacgcctaa taaagtggaa ttagactatt tacaagaatg cgaattagtt
      541 gaaccagacg ttaaaatgta tgttcaaatg tcaggcgatt ttggaactat cgcatatagc
      601 tggaatgcag aagatattaa agtagttggt tctaatagtg cacttaaaca aaaataatta
      661 ggtgatgtaa atggattatt atacagcaga tagattatat cgctacacaa atagttcaaa
      721 tttgagtgaa cctattttaa actatgttgc tagtcgtata aattggggcg ataaagtttc
      781 attaatgact ttagctaaag aaatacagtc taaatttaat gatagttacg ttaaagaaaa
      841 tacggtaaaa ggtcggccta aaatttacgc tgacctttgt ctattgtgta tgagcttgag
      901 tgaagcagga catggcagaa tgctacaagt gaatttggaa gattgtattt atattggcga
      961 tattgatgtt taacatatag tattagtgat ttaacatgac cgtgtagctt ttagcgtatg
     1021 caggcgcaga aatgcaagca cggagtgcgt taagcatttg ggcggagcct gcgacaaata
     1081 cgcgttaaaa aagcgagaac ggtcatgtat caaatcgctt gcgatttgat aggaggcata
     1141 aataaatatg actttaaaaa accagtgtac cccccttact aatagggggg tagagcgtac
     1201 aaataaaagt gctgtggagg cagttgttga ctgggttcag gtcactttcc atatagcccc
     1261 tatttcagca gtaattgagg acgttatcgg tttgcctata acgttgttca aaaaacgaaa
     1321 tagtggcatt tacttttata atcgcggtta tgaattttca aacattaaat tgtattattc
     1381 aagtgatgat gaatcaatgg gcattcactt acagttaact ggtacaggtt gtagagagtt
     1441 tgaacatcat ttacagcagt tgaataaaac ttggcaagat ttttttgaca aatgtttatc
     1501 agttaatgca aattttacaa gaattgatat tgcaattgat gattataaaa catatttaaa
     1561 agtgccttta ttaattaaaa aggctgaaaa agctgagtgt gtttcacgtt ttagagctgg
     1621 aagcgcaata aacggtttta atttgtcaga cggaagaagt aaaggcgcta ctttttatat
     1681 tggttctaaa caaagcaatt tatattgtcg tttttatgaa aagaattatg aacaagcgtt
     1741 taaacgtcat tgtgatgtcg aagacattgg gctatggaat cgttatgaaa tacaaatgcg
     1801 aaaggcatat gcagttaatt gtgcgaaagt gttgagtaga acagataata taagcgaaat
     1861 tgtaaagtct atattgcata ataatttgcg ttttatctct cctcctaaag gtggaaatga
     1921 taaaaatcgt aagcggtggc cattatatag accttgggcg ttatttatta aagatactga
     1981 aaaattgaat ttaactacta gaccaacatt aaagtccatt gaggataatc ttgattggtt
     2041 atgtaaacaa gttgctacta ctttagatac agtgttaact gccgaaagta tggcgcaatc
     2101 tgaggggttg cttacagata ctgatttttt agataaaatt ttagcgcatt cacaatttaa
     2161 tgatgagcat acgaatagaa ttaatcatta tttggaggct ttaaaacaaa aaagcactta
     2221 tcaaaagata agtgttaaaa aaataactgg gatgtgattt ttattaaaaa tttcgtaaag
     2281 gattttagaa aatcgtttat gaaaataata agcaagtaca ttttaacacg attttctaaa
     2341 aacggaaagc attttgatat tcctaaagaa caacgccgag taattccaaa atttaaaaat
     2401 cgtcgtaaga tgattgtaat ttgtttttac tcattattag ctattttgtt aattctttta
     2461 ttagcttctt ttattaaagc tacaagagca aataatgaca gtaaagaagc agttaataaa
     2521 acgaatatga ttcaaaaaaa gtatgaagat aatgcgaata cagttcagta tagtcctaaa
     2581 ttaaagttat atgctgataa gtttattgat acgtatatga atatctcgaa agattcaaaa
     2641 gaattggaat ctagagaaaa agaattgttg aaatattttc catcagatta taaaaaacct
     2701 gatgaaaaaa tttcagatac agaacgaaaa ttgaatagta aagaatttta taatattaaa
     2761 cgtaaagata aacaaacaat tatacagtat attgttaatt atgatgtgaa tattactgaa
     2821 aagaaagaag ttaaagttaa gaagaaaaag aaaagtgaaa aagataaaga tgagtatgaa
     2881 acaaaaacag aagaaaagca acgtaaagta aatcaaaaca ttttaataaa tatacctatt
     2941 aagagtgaaa ataataagta tgttgttgtt gaatatcctt attttacgcc aatccctgat
     3001 agtcaattga atcaagctaa aatggttgag gataatttga aagacaataa acgggaagac
     3061 aatccgaaag caaaagcttt tattgaagat ttctttaata aatatgcttc tagtaagtct
     3121 gatgatatgg cctatttaat ggataaccct gagggcttag aaggaactag agaagtttct
     3181 cagataagag aaattaggtt atatccaaaa ggcgatgatt atgttgctaa agttgaaatt
     3241 ttgatgaaag ataaagattc tcctttggag aatttggagc attacacatt agatataaca
     3301 aaaaaagatg gtaaatatta cgttaaaaat atgactaatt ctattggagg ttaatgttat
     3361 gagtggttta tttaattttt ttgttttagg tgctgaccgt ccatcacttg gtggagttgg
     3421 tgactggata agtaatgaag taggtacggg tattggttta gtagtattgg ttgttggtat
     3481 tgtaaaatgg gctagtggta aatatgggca tatggttata ttatttgttg ttggtggctt
     3541 tttattctta gtttctaaag gtcctgaaca ggtatttaat gcgattgcag gtgtttggaa
     3601 aatgattttt ggcggttgag gtgattccat ttggataaaa aagcttataa tttaaagcga
     3661 acttttgaac aaccgatagt tatgtatgaa tttacagata agtttcgtat taataagggt
     3721 tttcgtttag atttttgggc tacatttttg attgtgtggt ttatattatt tttgttattt
     3781 tggtatttat tacagcctgt aattatgtcg attggtggac ttatgtttat ttactttacg
     3841 gttgcacctt attatataac gaaatatatt gttaagttaa aacaagatgg aaagaaatta
     3901 tttttcttct tatgggattt tgtaatattt gtttttaatg tgcagttaag aaaagttaaa
     3961 ttaagttatg atgaagaagt agaatatcat gataaaaaaa taacatttaa atagccccta
     4021 ttggggttat ttttattgga gggatgacat gaatttaaaa gcaccgtatg caggtttaag
     4081 agataatttg ttattaacga aaactggcga agtttgggcg tattatcgtg ttcgttctga
     4141 gtctatatca acagcaaatt atgatgcaaa agaagaatca aaaaagcgta tgcgttattt
     4201 tttagagtcg attaaaggtt atcaagattt tcattttgaa atgtatgacc acgatttaga
     4261 tttgcgaaat aaatttaaag aaattagtaa agattttgat gatgaagctt ttaatgtagc
     4321 tgaatattat tcaaaagaaa ctattgacgt attagaacaa gaattacaaa tgattacgca
     4381 taattctttt gttatgggtg ttaaaatacg tgaattagct gacgatgcag ttacacttaa
     4441 agagatttta aaaagtacat tatcagatac ggctgagcgt attgtttcga attttggttt
     4501 tgatgtgaat ttagacgata aaattttaga taagtataaa acatttgaac gagatttggc
     4561 tgatagtttt ctaggtattg gaggcgagcg tttaactgag aatgaattgg cttatattat
     4621 tagaaagcct tttatttcaa atgcaacaca tgacgtacag gaagaatctt caagaagagc
     4681 tattacagat atttataatg cagtaattga ccctactcgc ttgagtgttt tagaagttga
     4741 aaatgataac gagaaatttt atacaacaac agttgttgtt gatgattttc cgttagatat
     4801 ggaatataca catttatttg agaaagctca aaatatgcct tttccggttg aatgccatgt
     4861 aaaagcaaaa atcattaata atgagaaagt taagaaaaaa tttgaccgtg cgaaaataaa
     4921 tcataaacaa caagcaaaag aaaagcaaga tactggcgac agtgcttcgg aagatgttca
     4981 agataatttg tttgtattga atcaaatgga acgagaagtg actggctcag gtgtatttat
     5041 tgagtgggta tttatgtttg taattaaaag tgatgattat agagattgta aacgtaaagc
     5101 aaagcgtttg atgaaacgat taaaggaaac tcaaatatat gcagttaatc cattagctga
     5161 ccagttacaa ttgttttatc aatgtttaca tggtaatgat ttatttatta ataagtattg
     5221 gttacaaaga acaacggcaa caggtatagc agagaattta tttggtgtgt ctcaaagttt
     5281 gggaacaaaa acgggttttt atatagggcg tattgataaa tttatacgtt ctgtttcacg
     5341 tgaggaagct gttgcaagtt caagggatat aattttgttt agtttattgt tggccgcaaa
     5401 aggtattaag ggggctgtca gtgatagtcc gcatgtttta attactggtc aaactgggaa
     5461 aggtaaatca tttttagcaa aattattatg gatttataca tcatttttta aaggtcaaat
     5521 gttgtactgg gatccaaaat ctgagtttgc agaatggttt gacagagtaa cagaaagtaa
     5581 ggaaatgcaa gaaaagtatc ccctatttat taatcattta aaaacattta aatatgtaac
     5641 attgaattat gaagattcaa ccaattatgg ttgtctagac cctattgtat ttttagatgg
     5701 tattgaagct aatgagatgt tgaaatcagt gtttgatgaa attaaatctt ttgaaaatta
     5761 tcatcatatt gaaacagcaa tttatcaagc tatttctgat acggttgaag aacgtgaaaa
     5821 tggtaaaaaa gttggttcat taaatgtaat tgaaaaatta cagaataatg aagatgagca
     5881 tgtaaaaaat gcaggcgatt tattatttga aaaaacgcaa aacaatattt taaaacttgt
     5941 ttttagtgat ggtacgaatc cagctttaaa tattcaagaa aaagcaacta ttttacaggt
     6001 taaaggttta gatatgccta aagcagatga tgatacatca agttattcaa caagtgagaa
     6061 aaatgggatt acattaatgt tattaattgg taagttttta gagaaattcg gttctcgtag
     6121 agatgttcaa acgactatat ttattgatga gggttgggct tttagtgctt caagacaagg
     6181 taaaaaagtt ggtaagcgta taaaaagaac gggacgttct gagaataatt cacttgtatt
     6241 tattacacaa tcggtaaaag ataaagcaga tgatgatggt ggtaactttg gttgtcattt
     6301 tgcatttgat gaaaaagatg aacgtgagga tattttaaaa tctttaggct tagaatattc
     6361 aaaagaatct cctgaaaaca tggagatgtt gaaagatttg aagaaaggtc aatgtatatt
     6421 cagtgatttt tatggacgtg ttggaaaaat ggttgtacat tgtccatttg aggaaatgac
     6481 tgaagcattt agaacacaag aagattctgc aagctctaaa gctgaagaaa aatttgcgat
     6541 gtaattttta gcacatacaa aattgtatgt gctttttatt ttaaaggagt ggatttgatg
     6601 gcaatgaatg aaattgcttt atttaaaggc gtacgtatac agccttatca gaagtatatt
     6661 gattttatgt ttgcaagtgt attagcattt atttttttgg tatatcgtat atatattttg
     6721 tttattgatt ataaatcagt taataaaaaa ttggatatat tgacgttgtt tgcatatttt
     6781 aaaccgcatt tattgtattt attaattggt acagcaatta tatttgtgat atgtaagctt
     6841 attgctcagt ttttaatacg ttttaaaaca aaagatttgg ctgaaatgat tgaaacacaa
     6901 ggttttcata atcgtacagt aatcaagaaa acaactgagt atatccctat tgattataaa
     6961 caaactgaat atgattatta tccaacaatg ttttataagc gtggtaaaaa atcttttgtt
     7021 attcatatta agaaagacgg ttcgaggttt caagataatt atttagagtt agagacgatt
     7081 attgaaccaa tgtttaattc tgaattagta gaaaaaaagc atattggtcg ttatttgaga
     7141 tatgaattta tgccacttaa atataaaaag cgtattgtta tgaatggcga acaagaatca
     7201 aatacaattt tttatgatac aaaaattgct attacgcatc aaattacttg ggattttgtg
     7261 aaagcacctc atggattggt tacaggtata accggaggtg gtaaaacata ttttttattt
     7321 tatgtgattc gtgagttatt ccgtagacat tcagaagtac gtttattaga cccaaaagtt
     7381 tcagatttga gctttatgaa gcgcgttatt ggtgatgata aagttgcaga tactaaaggt
     7441 caaatattaa aacaattgag agaagctaat aatgaaatgg aagagcgttt tagattaatg
     7501 aatgatagtt cggattataa aatcgggaat gatttccgta attttgatat gcgcccctat
     7561 ttcattattt ttgatgaggt aacagctttt acttcaactt tagataaaaa agaattacaa
     7621 gaaatgaatg attatttaat taatattatt atgaaaggtc gtcaagctgg tgtatttatg
     7681 tttttaacag cacaaagacc agatgcagat gtgattaaag gtaatgtgcg tgaccaatta
     7741 gggttacgtg tgtctttagg taatttgtca aatgatggtt atcgtatgac ctttggtcaa
     7801 actgataaag aatttcaaac gattcatgat agtgatatcg gccgaggtta tatttctata
     7861 cttggacaat ataatgaacc aattttattt gatgcacctt taatggaaca atatgatttt
     7921 gtggaagatg tgaaacaaat attaaataag gagtgatttg atgaagcata aaagtgtgta
     7981 tcttttatgc tttttaattt tgcctattct gcttttaaca tctgtgagtg cttatgctgt
     8041 ttctaatcca gttggagagc cctcacatgc agttaagcca aaaattgaaa agtatgattt
     8101 atcacattat cgttcaatat atagtgaaaa aggcgactgg aatccgtttg gtcaagaaga
     8161 aatttcacgt caaattaata atgtgtcaga ttttttcttt tctatgacta agattttagc
     8221 aggtgttaca gattatgcga ttgaaaattt atttcaatta gatgttatta atgattttgc
     8281 ggataaaatc ggcgattttg ttggcgatat atatcaaaag ttattgagta atttagcatt
     8341 aacattattt attattgttt gttttaacgc atttattatt tttagtgtac aaggtaatgc
     8401 aagagaagca ttaaaacgag cgtttttaat tatgtgttta attggttttg gtgttggtat
     8461 acttgcgaat gcaggtaata ttatacgtgg tacaaacaat attggtaagg acttaaataa
     8521 tataattatg aatagtacat cttctattga tggtaatatt gattatattc atgagaattc
     8581 aggtatgaat catatacgta atcaattatt tgatatgacg atatatagaa catatttagt
     8641 aatgaattat ggaactgtcg acgaaaaaga gataaaagca aaaggtaaag accgtattga
     8701 taacatttta aaacttgatt atgataaaaa atcagaaaaa gaattagatg atattgttaa
     8761 agatgaggtt gaaaagaaaa ataataaata tatgacgcaa ggttatgtat ttcaaaagtt
     8821 ggctatttca gtgattggtt ttattattac actttttatg tcgatagtat ttttatcaat
     8881 aagttttgcg aagttgattt tttctacatt tgctttattt ttattcttat ttttagtatt
     8941 cagttggata gtgagtttta taccaggatt tgaattatct gttttctctg cgtttgcaaa
     9001 aacgttaggc tatattattt taagtgcttg tatgacattc ttatttgtga tagttggttt
     9061 gtgtatcaag cttgcaaata gttttattga accggatagt caaaatgctt attttttaaa
     9121 ttcaatattt ataattgtaa tattgtttgt aatgtataaa aaacgggctc aaattatcaa
     9181 ttttgtaagt cgtggtaata ttagtttttc tcctagtgca attggtgctg gtgtgatgaa
     9241 tcgaacacaa gaacgtttta ataagattcg tcatgaacaa gcgcaaaata agaaagctaa
     9301 acgagaaaat caaagagatg aacccgcccc accacttcaa aatgataatg atttaagaag
     9361 aagacaacag gataaaccaa tgcctttatt tataaataaa gataatcaaa aaaatggtaa
     9421 taagcgcaga gaacaacaag agtcaatgaa tggtaacgat gtaaaatctg cttctgttga
     9481 atcaaatgca aataattatt ctaaacaacc acaaaaagca tcacagcaag aacatcaagt
     9541 acgagaaaca agacagcgaa aagatataca aaggtcgcca caagtggtta atcagccatt
     9601 aaataatgag aatcattcaa taaatcgtaa agaacagaag tcagttcaaa ctgcttatga
     9661 tactgatgtt caaaaacgac aaatacaaaa tgcgacacag aatcaacaaa gtagacaatc
     9721 tggaaatcgt aatcaaccta ttacgcgtaa ttctcagtca aaagaccgtt taaaagagca
     9781 aaaggatatc aataagcatg gtaaataaaa aagatattgc taaaaaagta ataggtaaaa
     9841 cgccaatagg cgttaaattg aaattagcga aagtcatcat cattttatgt gtggtggctt
     9901 tttttatatt tccagtaatt ataatgtttt tgttagcgcc tgatttaaat aaaggtaaag
     9961 atgatgctgg ttgtactgtt agtggcggta atgtttcggc aaatggtatt gataagttta
    10021 acgaaaatgc taaaggtgga aaattagagg gtaaaggaaa agaaatacag aaaattgcag
    10081 aaaaaaataa agtacctgtg aatattttta tggccattat tgcgagtgaa tcacaatggg
    10141 gtaaaggaga aaatgcgaca agacaaaata atcctttgtc tgttatgggt tcaaaatcga
    10201 ttcatgattc aacatatcca actattgaag atggtcttaa tgcgggagct aaaaatttgt
    10261 atgatgttta tatttctaaa ggtttagata ctcctaaaaa aataggtccc aaatatgcgc
    10321 ctgttggtgc gtcaaatgac cctaataata tgaatgcccg ttggatacca acagttgaaa
    10381 aaattatgaa agatttaggc ggttcagaag ctaaaacgtc atgtagtaat ggtaaaggaa
    10441 agtctataaa gtttaatggt aaattgccac attggtcaaa tgatgaccct ggaaaaggta
    10501 atttatacac tgctggtcaa tgtacttggt atgcatacgg tatgcgtcaa aaaatgggta
    10561 aacctgtatc gacttattgg catgatgcac ataaatggaa tgatagagct aaagctgagg
    10621 gttataaagt tgataaaaat ccggaacctg gtgcattatt tattgctgaa caaggtgcag
    10681 gcggtcatga tgggcattat ggtcatgtgg ctgttgttat tggtgtaagt gatggtggta
    10741 aaacatttag aataacagaa atgaattggg aaggtgcttt taaagttaat gaacgtacat
    10801 tgaaaatgac tgacggatat agttttattc atgataagga gtgaaaataa atgaagcaaa
    10861 aaattattat gtttactctt gtaacagtaa tattattttg tgctgtttta attggttatc
    10921 agattcctaa acaacaagtt aaaatgaaac aaaatcaaat tgaagatttg caggaagaac
    10981 aacgtatatt aagagataaa aatggagagt taaataaatt agtaaagaga caatcaaaaa
    11041 cagttatttc tgatgaagaa aaacaaatac gtgaagtttc ttcaaatttt gttaaacaga
    11101 tgtttgaaat gaaaaaagat tcttctttta aatcaaaagc accacaaata aagcctttag
    11161 ttacaaagga ttattacgat acattattta aggattctaa agataaatat gatttatatg
    11221 atgatataac ggttaatgat attcatgttt attttgatac ttatgaccct aaaaaagata
    11281 gttataaagt ttttgttcag ttcgacgaac gtattgaaac agatggagat gataaaattg
    11341 aacatcgtca aacatctgca caattggatt tagttcgtac agctgaaggt tggcgtattg
    11401 ataatttaaa acgtttcaat ttaaaacctc taggacgtta gtttgtttca agacgatagc
    11461 aatgatataa tttacgtaaa aaggagtgaa tttatcatga ggcgatggtt tgtattaatt
    11521 ttaggattag ttatattatt atctgcatgt ggtcagaaat atgataaaga aattgatgaa
    11581 gttacaaagt tagaaaaaga aagtattcaa gatgtgaaaa atacaaagaa gtataaaaat
    11641 gttgagcggt caaaaagtta ttataagatt tataatgatg gtgaggttat tataatgact
    11701 tatatgcctt ttaaagatag taatacaaaa gtttcaagag tatataaaat aaatcagact
    11761 agcgataaat atgaagaaga ttctaatatt gatgctgaaa agtttgagaa agataataaa
    11821 ccagtttatg aagaaaataa tatgaaaaaa taaagaatca actccctata cgctttagcg
    11881 atggtatcgg gagttgatga acatatatcc tcttataact tattttataa ataacctttt
    11941 taaaagtaaa ggaggatata catgaaaaaa agaaaaaaac gtaaagaatt agactataaa
    12001 acacgtctag ctatcgcaaa gtatttaaat gatggtttaa acatcattca gatttctaag
    12061 attttaggtc gtgatgatgc agttctttat agagaagtaa ataagcgttc tgttgatggt
    12121 gtttataatc ctttattagc tgaggaagat gctataaaat cgcgtagtat ttcagatgaa
    12181 caagttgcta aacgtagacg tttgaatgat gaaacaaaag catatattga agaaaaactt
    12241 tcattaaaat ggtctcctag acagatagca agtcaaattg aaaaagatac agggtattat
    12301 ataagttatc ctactatata tagatatatt cgcaatggat tggtaaaagt tgatgttaag
    12361 cgtgatatga gacaagcagg caagaaatat aataagtctt ctgaaaagcg tggcaaatta
    12421 gacgtagggt ctcgtgttat aaagtatcgg ctcaaaaagt attaataatc gtgaagaatt
    12481 tggacattat gaaattgata ctgtttggag tgttagacca tcaacatatt gcttgttaac
    12541 tattattgaa agaaagacaa gatatttata tgctagtaga ttaaatacac gtaaatccaa
    12601 tgtagtttgt aatgagatta taaatattat gaaaccatta ttacccaaaa gtataactat
    12661 ggatagaggt aaggagtttg ctctatttga agaaatagaa gatgaattga attgtagtat
    12721 ttattttagt gatttgggtt gtccatatca acgtggttct attgaaaatg caaatggact
    12781 tttaagacag tattatccta aaggtacgga ttttgcaaat ataacacaaa agtcattaga
    12841 tgaagctgtt aaacaaatta atgcacggcc tcgaatgata tttgactata aatcgagtga
    12901 ggaaatgcta aagtaccatg taagcacaca aaattgtgaa cctatattaa atgactgtgt
    12961 gaggcacgaa cccgtcaatt aatataggtt aatctaagcg aatggtttac aatgagcgcg
    13021 gaaggacagg acgaataaat tttgtatgta aaatgcataa tctaaaaaga agcatactat
    13081 ttaaatctgt ggtttttgac agatattact agtaaaatgc ttctttttct tcttctgtac
    13141 tcttttagta atatttatta aaaaaattta agaaattctc atttttctat tgacacactg
    13201 ccatttaatt aaagcaattg gagtattgtt actcattgtc ggcatcgtca taatgaatca
    13261 atttaacaag aataatttat tactaactga tcaaaagtat ttactgtttt ggcttctatt
    13321 aggatttatt tttggtttct ttccacctat tcaaacgaca attaatagtg ctttagctag
    13381 tcatactcat tcaccagcct ttgcatcatt agtatcattt acaattgggt caatagcgct
    13441 attgatttta accgctattt ttaatcgttc tttaaaacta aaaacaagtc atttaaaatt
    13501 cggtaaatta aagcctatct attttactgg cggtatactt ggcatggctt ttgtaacagc
    13561 taacattatc ttaatgcctc atatgggtgc agcattaaca acacttattg ggatgtttgg
    13621 ccagattcta atgggcatat tgatagatca ctttggatta tttggttcac ctaaaatagc
    13681 aatgacatcc agaaaaacta ttggtctatt atgtattttg acaggcatta tacttttaag
    13741 attattttaa attaactttt agcttatcat tttaacttgt aattattttt aaaagtgata
    13801 agctattttt ttgtggtcta aaaatcttta gaaaagcgtt gatttgggta gtatgctttg
    13861 acacaacaaa ttttaattta gcaaattcga tagtcaactc attcttaaga cctaaattaa
    13921 tgttattttt taataattta caccaaatta atagcaaaaa ttatgttatt cgtgctaata
    13981 tttcatagtt ggttattcaa ttaattaaaa ataagtcaaa atgcacaact ttttataatt
    14041 cattgagtcg agtttgaaaa ataaaagtgc tttaatgcat gatcaattat cgtactttct
    14101 attatttgtt acccgttatc aatcggaata acgtatagac actttaacgt gctatagatt
    14161 ggttttaatc actaaattaa tgtgtttttc tt
//
LOCUS       DALAGF010000073        13529 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.073, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000073 DALAGF010000000
VERSION     DALAGF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13529
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.073"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(303..1055)
                     /locus_tag="O2692_001938"
     CDS             complement(303..1055)
                     /locus_tag="O2692_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HCZ4299561.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRIVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFDQFFAIS"
     gene            1223..2209
                     /gene="pta"
                     /locus_tag="O2692_001939"
     CDS             1223..2209
                     /gene="pta"
                     /locus_tag="O2692_001939"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCZ4299562.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            2212..3048
                     /locus_tag="O2692_001940"
     CDS             2212..3048
                     /locus_tag="O2692_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCZ4299563.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            3635..4555
                     /gene="mvk"
                     /locus_tag="O2692_001941"
     CDS             3635..4555
                     /gene="mvk"
                     /locus_tag="O2692_001941"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCZ4299564.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            4560..5543
                     /gene="mvaD"
                     /locus_tag="O2692_001942"
     CDS             4560..5543
                     /gene="mvaD"
                     /locus_tag="O2692_001942"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCZ4299565.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            5556..6632
                     /locus_tag="O2692_001943"
     CDS             5556..6632
                     /locus_tag="O2692_001943"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCZ4299566.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEETDQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            6809..7150
                     /locus_tag="O2692_001944"
     CDS             6809..7150
                     /locus_tag="O2692_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HCZ4299567.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKVEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(7202..8524)
                     /locus_tag="O2692_001945"
     CDS             complement(7202..8524)
                     /locus_tag="O2692_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCZ4299568.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSPKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHDDIVDSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAYGITDLENKGIAL
                     HTNVETTELSSDNHHTTVHTNVDNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(8511..8951)
                     /locus_tag="O2692_001946"
     CDS             complement(8511..8951)
                     /locus_tag="O2692_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCZ4299569.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYNGKTIKDVIEDIQKEDLCHENI"
     gene            9009..9110
                     /locus_tag="O2692_001947"
     CDS             9009..9110
                     /locus_tag="O2692_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299570.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVDALTVRLHN"
     gene            9576..10985
                     /locus_tag="O2692_001948"
     CDS             9576..10985
                     /locus_tag="O2692_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCZ4299571.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINGNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWV
                     ILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMDSVGNA
                     VSEAKNIISNHINPMKWAW"
     gene            10989..11615
                     /locus_tag="O2692_001949"
     CDS             10989..11615
                     /locus_tag="O2692_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ4299572.1"
                     /translation="MLFNIKVINKNPRYKVVQYNDEYLLIDLVSTWLVYFFPFINWFI
                     PKRYAKLSEKELENLNVDKQNKNNIFWPVTGSSFLFVVILRKYVHTFEVQLDNKILIS
                     LCFIGFIGIAAFYIYLNKKLKLKIYDDNLDNENRVILVPTFKDGSFIVFTYLLLGGCS
                     ILFLIWLMTIKPQNLLVFIMWIIITIFFFLISMGSISNKKVYAKLKKQ"
     gene            11741..12384
                     /locus_tag="O2692_001950"
                     /pseudo
     CDS             11741..12384
                     /locus_tag="O2692_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499153.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
     gene            12472..13089
                     /locus_tag="O2692_001951"
     CDS             12472..13089
                     /locus_tag="O2692_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ4299573.1"
                     /translation="MLCETEIINKNPKYRVIKYDDEYLMVDVIRTWLVYFFPFINWFI
                     PKRCAKISREEYEKLNTVKPVKNKNFWPVVGGTILLGATSRKYIHLLNIQLEKRSVIF
                     ICFVVFLCILIFFVLLNRKLKLKVFDNKKEEQKIILVPTLKNAVLILYGYLLIGGMSI
                     LALSMLLTLENQNLITFIAWGMGLMLFFLMNITLIVNKTVKVIKR"
     gene            13164..>13529
                     /locus_tag="O2692_001952"
     CDS             13164..>13529
                     /locus_tag="O2692_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ4299574.1"
                     /translation="MLCESRVINQNPKYRIIKYNNEYFMVDLVSTWIAYFLPMINWFI
                     PKKYAKISREEFESLNIVKPAKNNTFWPVAGFAVLLTTLTRKYIYLLNIHLEKEIVIL
                     TCCMILLGVFALFIYINTKL"
BASE COUNT         4797 a         1848 c         2514 g         4370 t
ORIGIN      
        1 ccattacaaa aattgtatag agtagcgact gtataatttc tattgaggtt aacgtttata
       61 tgtagtgata gtagttaaag ttctcccaag gaagactact cgggtacact ttgctatgag
      121 caaagtgtac tttgttattg ataatacatt agcacatata tataagttta aacatacaga
      181 tttcaactat ttaccaaatc acgtcttatg tatacgggaa tatactgaag ataaacgaaa
      241 aattccaagc ttaaaccgat aagcttggaa tttttttatt aatttataaa cgtaccaatg
      301 tattaagaaa tcgcaaagaa ttgatcgaat tcgtttgtgt taataatatg tcctacaaag
      361 aaactaccga attcaccgta tcgtgctgtt gtttcatcaa agcgcatttc gtatacaatt
      421 tttttgaatt gtaatacgtc atctgagaac aatgttacgc cccattcgaa atcatcaaac
      481 cctacagaac cagtaataaa ttgtttgatt ttgccagcat attttctacc aatcatacca
      541 tggtcataca ttaatttttg gcgttcttcc atagttaaca tgtaccagtt ataagtttca
      601 ttacgacgtt tgttcattgg atagaaacaa atataatcag aatgtggtaa ttctgggtat
      661 aatcttgctt tgatatgagg gttctcataa ggatcttcat cagatttacc agctaaataa
      721 ttgctcaatt caatgactga tacatatgaa tatgtaggga ttaggaagtc agcaatgcgc
      781 aatttgttaa attcattttc aatatgattt aaagacttca tttcaggacg taagaaccat
      841 aataacaaat ctgctttttg accagttata ttataaatag cttgatcacc agattttgat
      901 gatcttacag ttgctgtatt ttctaaaaat gattgaaatt cagtgacaag tgcatcgcgt
      961 tcgtcctttg gaactatacg taatgatgcc caatcaactg cataaaataa atgtagacta
     1021 taccaaccat ctaatgtttc ggctgcttga ctcatgttta tcgctccttt tcaaaaatca
     1081 tcagttcttt ataaatttta tcataaagca aaggggtaac gataaaatta tgattacaaa
     1141 ttggtgacgt ggcattatga aataaaatgg cgtataatta taccgtgaat gattaataag
     1201 atttatatta caggaggaca ttatggctga tttattaaat gtattaaaag acaaactttc
     1261 tggtaaaaac gttaaaatcg tattacctga aggagaggac gaacgtgttc taacagctgc
     1321 aacacaatta caagcaacag attatgttac accaatcgtg ttaggtgatg agactaaggt
     1381 tcaatcttta gcgcaaaaac ttgatcttga tatttctaat attgaattaa ttaatcctgc
     1441 gacaagtgaa ttgaaagctg aattagttca atcatttgtt gaacgacgta aaggtaaagc
     1501 gactgaagaa caagcacaag aattattaaa caatgtgaac tacttcggta caatgcttgt
     1561 ttatgctggt aaagcagatg gtttagttag tggtgcagca cattcaacag gcgacactgt
     1621 gcgtccagct ttacaaatca tcaaaacgaa accaggtgta tcaagaacat caggtatctt
     1681 ctttatgatt aaaggtgatg aacaatacat ctttggtgat tgtgcaatca atccagaact
     1741 tgattcacaa ggacttgcag aaattgcagt agaaagtgca aaatcagcat taagctttgg
     1801 catggatcca aaagttgcaa tgttaagctt ttcaacaaaa gggtctgcta aatcagacga
     1861 cgtgacaaaa gttcaagaag ctgtcaaatt agcacaacaa aaagctgaag aagaaaaatt
     1921 agaagcaatc attgatggcg aattccaatt tgatgctgcg attgtaccag gtgttgctga
     1981 gaaaaaagcg ccaggtgcta aattacaagg tgatgcaaat gtctttgtat tcccaagttt
     2041 agaagctggt aatattggtt acaaaattgc acaacgttta ggtggatatg atgcagttgg
     2101 tccagtatta caaggtttaa attctccagt aaatgactta tcacgtggct gctcaattga
     2161 agatgtatac aatctttcaa ttattacagc agcgcaagcc ttacaataac gatggattta
     2221 gcgagtaaat attttaatgg cgtcaactgg cgatatatcg atcattcttc tggattagaa
     2281 cctatgcaat ctttcgcatt cgatgataca ttttgcgaaa gtgtgggcaa agatatatca
     2341 gataatgttg tgcgtacttg gattcatcaa catactgtta ttcttggtat tcatgattca
     2401 agattgccgt ttttaaaaga tggcattgat tatttaacga atgagattgg ttataatgcc
     2461 attgttagaa attctggtgg cttaggtgtc gttctagatc aaggtgtatt aaatatatcg
     2521 ctgatgttca aaggacaaac agaaacaacg attgatgaag cgtttactgt gatgtacctc
     2581 ttaattagca aaatgttcga aaatgagaat gttgatattg atacgatgga aattgaacat
     2641 tcttattgcc caggaaaatt tgacttaagt atcgatggta agaaatttgc aggcatatcg
     2701 caacgaagag ttagaggcgg tattgctgta caaatttatc tttgtgttga aggctctggt
     2761 tcagaacgtg cattgatgat gcaaacattt tatgaacatg ctttaaaagg tgaagtgact
     2821 aaatttaaat atcctgaaat tgaaccatct tgtatggcct cattagagac attgcttaac
     2881 aaaacgatta ctgttcaaga tgtaatgttt ttactattat atgcaatcaa agatcttggc
     2941 ggtgtattaa atatgacgcc aattactcaa gaagaatggc aaagatacga tacgtatttt
     3001 gataaaatga ttgaaagaaa caagaaaatg atagatcaaa tgcaatagtt aaatatttaa
     3061 cagccccttt caagttatcg tttggggctg ttatttttga aagtatccat aaaaagcata
     3121 aatatgttat tgacctaata tttaaattga acatcattaa attaaaagta atgatatgat
     3181 ggaaataata aatttaataa gaaaatttca tgttttacgt attaaataga ttgagaatag
     3241 ggtatatata atgcgtatga ctatgaaaga aacgaccttc tattgaaatg ttctaatact
     3301 ttagtagtaa atctatatat tccaattatt atttgtagta ttatagttag ggtggaaaat
     3361 tataaattgt agggctattt tataaaaaga taaagaatct gaaatatcac tcgataagat
     3421 ttaaactttg aagtaaacga aagttagttt tatactaatc aagttaagtc aaatataaat
     3481 tcaaaataaa gattatacat aatatgaata atgtgagata aagactgcaa tgaaatttaa
     3541 cgagaatgcg tatgcaactt agaatatgat gtcttttact aataaataaa aataatgaat
     3601 caagtatagg ataaatataa aaggtgatat tgacatgaca agaaaaggat atggggaatc
     3661 gacaggtaag attattttaa taggagaaca tgctgttaca tttggagagc ctgctattgc
     3721 agtaccgttt aacgcaggta aaatcaaagt tttaatagaa gccttagaga gcgggaacta
     3781 ttcgtctatt aaaagcgatg tttacgatgg tatgttatat gatgcgcctg accatcttaa
     3841 gtctttggtg aaccgttttg tagaattaaa taatattaca gagccgctag cagtaacgat
     3901 ccaaacgaat ttaccaccat cacgtggatt aggatcgagt gcagctgtcg cggttgcttt
     3961 tgttcgtgca agttatgatt ttttagggaa atcattaacg aaagaagaac tcattgaaaa
     4021 ggctaattgg gcagagcaaa ttgcacatgg taaaccaagt ggtattgata cgcaaacgat
     4081 tgtatcaggc aaaccagttt ggttccaaaa aggtcatgct gaaacgttga aaacgttaag
     4141 tttagacggc tatatggttg ttatagatac tggtgtgaaa ggttcaacaa gacaagcagt
     4201 agaagatgtt cataaacttt gtgaggaccc tcagtacatg tcacatgtaa aacatatcgg
     4261 taagttagtt ttacgtgcga gtgatgtgat tgaacatcat aactttgaag ccttagcgga
     4321 tatttttaat gaatgtcatg cggatttaaa ggcgttgaca gttagtcatg ataaaataga
     4381 acaattaatg aaaattggta aagaaaatgg tgcgattgct ggaaaactta ctggcgctgg
     4441 tcgtggtgga agtatgttat tgcttgccaa agatttacca acagcgaaaa atattgtaaa
     4501 agctgtagaa aaagctggtg cagcacatac ttggattgag aatttaggag gttaatgcgt
     4561 tgattaaaag tggcaaagca cgtgcacata cgaatattgc acttataaaa tattggggta
     4621 aaaaagatga agcactaatc attccaatga ataatagcat atctgttaca ttagaaaaat
     4681 tttacactga aacgaaagtc acttttaacg accagttaac acaggatcaa ttttggttga
     4741 atggtgaaaa ggttagtggc aaagaattag agaaaatttc aaaatatatg gatattgtca
     4801 gaaatagagc tggcatcgat tggtatgcag aaattgaaag cgacaatttt gtaccaacag
     4861 cagcagggtt ggcttcatcg gcaagcgcat atgcagcttt agcagcagct tgtaatcaag
     4921 cgctagacat gcagctgtca gataaggatt tatcgagatt ggcgcgaatt ggttcgggtt
     4981 ctgcgtcgcg tagtatttat ggtggatttg cagaatggga aaaagggtat agtgatgaga
     5041 cgtcatatgc cgttccactt gaatcgaatc attttgaaga tgaccttgcc atgatatttg
     5101 ttgtgattaa tcaacattct aaaaaggtac ctagtcgata tggtatgtca ttgacacgaa
     5161 acacatcaag gttttatcaa tattggttag atcatattga tgaagattta gctgaagcaa
     5221 aagcagcgat tcaagacaaa gattttaaac gccttggtga agtaattgaa gaaaatggtt
     5281 tgcgtatgca tgccacgaat ctaggatcaa caccgccgtt cacatatctt gtgcaagaaa
     5341 gttatgatgt catggcgctt gttcacgaat gccgagaagc ggggtatccg tgttatttta
     5401 caatggatgc gggacctaat gtgaaaatac ttgtagaaaa gaaaaacaag caacagatta
     5461 tagataaatt attaacacag tttgataata accaaattat tgatagtgac attattgcca
     5521 caggaattga aataattgag taaggaagag ataaaatgat tcaggtcaaa gcacccggaa
     5581 aactttatat tgctggagaa tatgctgtaa cagaaccagg atataaatct gtacttattg
     5641 cgttagatcg ttttgtaact gctactattg aagaaacaga ccaatataaa ggtaccattc
     5701 attcaaaagc attacatcat aacccagtta catttagtag agatgaagat agtattgtca
     5761 tttcagatcc acatgcagca aaacaattaa attatgtggt cacagctatt gaaatatttg
     5821 aacaatacgc gaaaagttgc gatatagcga tgaagcattt tcatctgact attgatagta
     5881 atttagatga ttcaaatggt cataaatatg gattaggttc aagtgcagca gtacttgtgt
     5941 cagttataaa agtattaaat gaattttatg atatgaagtt atctaattta tacatttata
     6001 aactagcagt gattgcaaat atgaagttac aaagtttaag ttcatgcgga gatattgctg
     6061 tgagtgtata tagtggatgg ctagcgtata gtacttttga tcatgaatgg gttaagcatc
     6121 aaattgaaga tactacggtt gaagaagttt taatcaaaaa ctggcctgga ttgcacatcg
     6181 aaccattaca agcacctgaa aatatggaag tacttatcgg ttggactggc tcaccggcgt
     6241 catcaccaca ctttgttagc gaagtgaaac gtttgaaatc agatccttca ttttacggtg
     6301 acttcttaga agattcacat cgttgtgttg aaaaacttat tcatgctttt aaaacaaata
     6361 acattaaagg tgtgcaaaag atggtgcgtc agaatcgtac aattattcaa cgtatggata
     6421 aagaagctac agttgatata gaaactgaaa agctaaaata tttgtgtgat attgctgaaa
     6481 agtatcacgg cgcatctaaa acatcaggcg ctggtggtgg agactgtggt attacaatta
     6541 tcaataaaga tgtagataaa gaaaaaattt atgatgaatg gacaaaacat ggtattaaac
     6601 cattaaaatt taatatttat catgggcaat aaatgatttg ataggggagc aatcatctaa
     6661 tttaattaga agataaatgc tccatttttt attgtcagcc gtgttacgta tcttgaaaaa
     6721 aatattaaaa agagtaaaat agatagagtt aacgaaaaaa ttaatgaaat cgacgatata
     6781 gatatgataa agaaaggtgg gtagcaatat gaaaaataca ttccttattt gtgatgaatg
     6841 tcaggcagtc aatataagaa cgttacaaaa gaagttggaa aaattagatc ccgatgctga
     6901 aatcgtgata ggttgtcaat cttattgtgg acctggacgc cgaaaaacat tcacttttgt
     6961 taataaccgc ccactggctg cgcttactga agaagaatta atcgaaaaag tttctcaaca
     7021 attaaagaaa ccacgtgatc ctgaagaaga agagcgttta agaaaacgac atgaagaacg
     7081 taaacgtcgt aaagtagaac aagatagaaa gcttaaagaa aaattagaaa agcgaaaagc
     7141 acaacaataa agcctgatgg cagcatcatt caatgcgtgc caccaggttt ttatgttttg
     7201 tctagaaatt aaataaatca ttaaatgatt cggccatcgt aggatgcgta taaatattat
     7261 ctcgtaatac ggtatatgga atgttttgat caatcgcaag tttaattata ttaattaatt
     7321 cttcagattg cttaccatat aatgtagcac ctaaaatcat attattttca ttattaatga
     7381 ctactttaaa taaacctctt ggatcattgt taattttatg acgaggtata gcacttacta
     7441 aaagttgatg ttcagtgtaa tcataatgtt gagcggcagc ttctttacta gttaatccaa
     7501 cacgtgataa tggtggatct ataaatactg tataaggcac gctgcctcta ttgtcagtcg
     7561 tacgtgactg attaccatat aacgctgatt tgataattcg ataatcatct aaagatatat
     7621 acgtaaattg aagtccgcct ttaacatcac ctgcagcata aatatgcggc acagttgttt
     7681 gaagatgagc attgacttta atttcgcctc tgtcgcctaa ttcgatatca gtattttcta
     7741 aagctaaatc cgtattcggt ttgcgcccga tagccaaaag tactgcatca gcctcaaagt
     7801 tatcaacgtt ggtatggact gttgtatgat gattgtcaga tgacaattca gtcgtttcaa
     7861 catttgtatg caatgcaatg cctttatttt ctaagtcagt aataccatat gcaacgacat
     7921 cttgatcttc gcgtggcata aatgattcgc cacgttctaa tactgttacc ttactaccta
     7981 aattcgcaaa cattgaagca aattctaagg cgatataacc gccacctaca ataacgaggt
     8041 gcttaggttg atagctaatg tttaataaac ctgtcgaatc gaagacgtgt ttagcttgat
     8101 caaggccttt aatgttagga atgacagagg tagcaccggt attaataatg atatgaggtg
     8161 cagtaatact atcgacgata tcgtcatgtt gatctaataa attcacttca gtattagatt
     8221 taaactgcgc tttaaaatcc agtacatcaa tgttgttatc gtctgctaat aagtggtaat
     8281 ttttattgtt tagcgcattg acaacatcgt ttttacggtt ataacttgct tcaaaagatt
     8341 tgccttctaa tccatcatgt acaagtgtct tcgaaggtat acatcctatg tttatacaag
     8401 tgcctccata cattttcgga gattgttcga taactgcgac gtgttgacct gttgatgcag
     8461 cgtatttcgc taaagtttta ccagctttcc caaatcctat tacaattaaa tcatatgttt
     8521 tcatgacata aatcctcctt ttgaatgtct tcaatgacat ctttgattgt ttttccatta
     8581 taaaaattaa taatgatatt ctgttcgtct tgctgataat gtgacatggt agttgcaata
     8641 ttacgagcaa tttgacagtg actgccttcg tcgccagtaa atagacgtgt gtggtgttct
     8701 ttctctaaga caaaatgttt atataatgtt gctagagaga catcagcact ttgatcattt
     8761 gctaaataac cgccatcttt acctcgtatt gtgtcaatca tttttaaatc gacaagttga
     8821 gtcgtcacgc gtcgtaattg aacaggattt aaacaagtta attctgctaa tgaactacta
     8881 ttgaattttt ctgaatgatg cttagttaaa aaagctaata catgcacggc aatgttaaat
     8941 tctaaattca atgtttctca cctcataatt gtaactgtat tagttacaat taaaaatcat
     9001 tttgctcaat gtgtcaatac ttatgtttga aaagtgttga attaggtgtg gttattgtga
     9061 tgatttattg tttgaaagtt gatgcattaa cggtgagatt gcacaattga aattttaatt
     9121 atgtatgaac tgatagatat gaaatgaata ttgaaaatga gagcaataaa attttgaatg
     9181 atgatgttgt gtgattgtat aaacttattc gtattttaga tacgttttga tatttcgcat
     9241 gatgaggaaa tacttttaac aactgatgac atattttaat agtgaatata ttttgagata
     9301 ttaatatatt taatgttaag gatgttgtcg caataaaatt aaaatattga aagataattt
     9361 ttattgatta attgcgaata tacaaagaca ccattatatc catatgctaa aataagaaaa
     9421 aaggcgacat gttatctcaa tttacctgca tctagactcc tttacataag aaataatcat
     9481 cgccgagttt taaaatttta caactttgta gatgtaaaaa gcatttgatt ttaaatagtt
     9541 tgaaaatcat atatttttga aaaaggagca tgccgatgag tattgacatg tatttagaca
     9601 gatctcgaaa ccaagcttca agtgtgggga atttgagtca aacaatgaat tcaaattatg
     9661 atgcgttgga aaaagcaatt actcaattta ttaatgatga tgcgcttaaa gggaaagcgt
     9721 atacgtcagc taagcaattt tttagtacgg tgttaattcc attatcaaca agtatgaaaa
     9781 cattgagtga tttaacgaag caagcttgcg ataattttgt gtcacgttat acgagtgagg
     9841 ttgatagcat atctttaaaa gaatcagagc ttgaagaaga tatcagatca ttaagtcaac
     9901 aaattacgcg atatgaaaat ttgaataaca atttgaaaaa gcatgcttcc gataatcagc
     9961 aagccatttc atcgaaccaa caaataatac gaacattagg tcaacaaaaa catgaattag
    10021 aagagaagct acgcaaattg cgtgagttta atcaaaaatc accagaaata tttaaagaag
    10081 ttgaagaatt tcaaaaaatt gtccaacaag gacttaccca agcgcagaat ttttggaact
    10141 tttcaacaaa tcaatttaat attccttcag gtaaagaact tgattgggcc aaagcaagtc
    10201 atgaaaaata tttgaaagtt gctatgggga aaattgaaca taaagcagag aaagaaactt
    10261 taaataaagc agactttgct gttataaagg catatgccaa agaacatcca gaagacgata
    10321 tcccgaaaag tatattgaaa tatataaatg gcaataaaga cagtattaaa agagatatag
    10381 gattagatat tacgtcaaca cttttagagc aaggcggtat aaatgcaagt aaattcggtg
    10441 tatttatcaa tacagcaggt ggagtgaaag gcccagcagg tccaaattca tttgtggaag
    10501 tcaaacgtac atcaggtaat gtgtttatag aaaatggtag taaatttgca aaaggcggaa
    10561 aatacctagg taaaggtgtt gctggtgtag gatttggtat aggtatgtat gatgaccttg
    10621 caaatgatga taaaacattt ggagaggcgt tgtcgcataa tggtatgacg cttgcagctg
    10681 gatctgcagg gacagcagtt ggagctggat tagcgacttt tgttttagga agtaatccag
    10741 taggatgggt gattttagca ggtttggcta tgagtacagt atttgcatta ggaacagatt
    10801 taatttatca aaataatatt tttggattaa aagataaagt agactgggta gggcataaaa
    10861 tcgataatag tatagatgtt gttaaaaaaa ctacagaaaa atccatggat agtgttggga
    10921 atgctgttag tgaagctaaa aatattatta gcaatcatat aaatccaatg aaatgggcgt
    10981 ggtgagtttt gctttttaat attaaggtta ttaataaaaa tccgagatat aaagtcgttc
    11041 aatataatga tgagtatttg ttaattgatt tagtaagcac ttggcttgta tacttttttc
    11101 cttttattaa ttggttcatt cccaaaaggt atgcaaaact tagtgagaag gaacttgaaa
    11161 atttaaatgt tgataaacaa aataaaaata atattttctg gccagttact ggtagttcgt
    11221 ttttatttgt agttatttta cggaaatatg tacatacgtt tgaagttcaa ttagataata
    11281 agattttaat atctttatgt tttataggat ttataggtat tgcagcattt tacatttact
    11341 taaataaaaa gcttaaacta aagatatatg atgataatct agataatgaa aatagggtta
    11401 tattagtgcc tacttttaaa gatggaagtt ttatagtttt cacatatcta ttattaggag
    11461 gttgttctat attattttta atatggctaa tgactataaa acctcaaaat ctactggtat
    11521 ttattatgtg gattattatt acaatttttt tctttctaat aagcatgggc tcaattagta
    11581 ataaaaaggt ttatgccaaa cttaaaaagc aatagaaatt actaaactta gattgtagtt
    11641 cgtaagttaa gtaataaaca ggaaaatagt atgttactaa aatacaaaac acttataaag
    11701 tatctgaaag tgtattagat ataaaaccaa cgagacggaa gtggagatat tgctttgcga
    11761 ggttagagtt gtaaacaaaa atcccagata tagaattatt aaatataaaa acgactatct
    11821 gatgattgat ttagtaagca cctggttagt tttgttcttt ccgtttatta attggttaat
    11881 accaaaaaaa tatgtccaaa tcagcagaga agattttgat aatttaaata ttgtcaaacc
    11941 agttaaaaat aaagctttat ggccagctat aggtagcata cttttatttg gcactatgtt
    12001 tagagataaa atatatatat acctgattct catttagaaa aaaattgcgt tattatcatt
    12061 tgttcggttt tattattaag tattctagtg ttttatatat atttaaatca gaaagtaaag
    12121 ttatctatct ataatgatcg gagtagcaat ggaaaaatta tgatttttcc atcattcaag
    12181 aatctttgct ttgtactatt ttcttatttt ttctgtggtg gattatcaat catgttctta
    12241 gatgttttga ttagtttatc tatccaaaat attatagtat ttattgcttg ggttattatg
    12301 acaatgctat ttttctttat aaatatgtct tcaataatag acaaaaagat tcatgttata
    12361 tatttaaggt catataaata ttaattttga tacaaaaggg cacaagtgtt ttgagaaaca
    12421 gaaacgataa tatagctgtg tgaatttgtt attaatagga agtgataatt attgctttgt
    12481 gaaaccgaaa ttatcaataa aaatccgaaa tacagagtta ttaagtatga tgatgagtat
    12541 ttaatggtcg atgtaataag aacttggctt gtatactttt ttccatttat taattggttc
    12601 attccaaaaa ggtgcgccaa aattagtaga gaagagtatg aaaaactgaa cactgttaag
    12661 ccagttaaaa ataaaaattt ttggcctgtt gtaggaggaa caatcctact aggtgctact
    12721 tccagaaagt acatacactt acttaatatt caattagaaa aaagatcggt tatatttata
    12781 tgcttcgttg tatttctatg tattttaatc ttttttgtgc ttctgaatcg aaaattaaaa
    12841 ttgaaagttt tcgataataa aaaagaagaa caaaaaataa ttttagtacc aacattaaaa
    12901 aatgctgttc ttatattata tggctatttg ttgattggag gcatgtcaat attagcttta
    12961 agcatgttgc tgacattaga aaaccagaat ttaataactt ttatagcttg gggtatgggg
    13021 ttaatgttgt ttttcttgat gaatataaca ctcattgtca ataaaacagt caaagttata
    13081 aaaaggtgaa taaataacta attatattgt attggccgtt cttcaaataa aaaataattt
    13141 gaataaggtg gaagtgataa acagtgcttt gcgaatctag agtcattaat caaaacccta
    13201 aatatagaat tattaaatac aataatgaat atttcatggt tgatttagta agtacttgga
    13261 ttgcttattt tttgcccatg attaattggt ttattcccaa aaagtacgcg aaaattagta
    13321 gagaagaatt tgaaagttta aatattgtca aacccgctaa aaataatact ttctggcctg
    13381 ttgcaggatt tgcagtgtta ttaacaacct taacaagaaa atatatctat ttgcttaaca
    13441 tccatttaga aaaagaaata gttatattaa catgctgtat gatacttcta ggtgttttcg
    13501 cattgtttat atatataaat acaaaattg
//
LOCUS       DALAGF010000074        13449 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.074, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000074 DALAGF010000000
VERSION     DALAGF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13449
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.074"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            269..1180
                     /locus_tag="O2692_001953"
     CDS             269..1180
                     /locus_tag="O2692_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HCZ4299575.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            complement(1320..4772)
                     /locus_tag="O2692_001954"
     CDS             complement(1320..4772)
                     /locus_tag="O2692_001954"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCZ4299576.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFNIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(5326..6552)
                     /gene="ftsW"
                     /locus_tag="O2692_001955"
     CDS             complement(5326..6552)
                     /gene="ftsW"
                     /locus_tag="O2692_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCZ4299577.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            complement(6866..7141)
                     /locus_tag="O2692_001956"
     CDS             complement(6866..7141)
                     /locus_tag="O2692_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCZ4299578.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            7282..7764
                     /locus_tag="O2692_001957"
     CDS             7282..7764
                     /locus_tag="O2692_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299579.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            7766..7933
                     /locus_tag="O2692_001958"
     CDS             7766..7933
                     /locus_tag="O2692_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HCZ4299580.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(8073..9920)
                     /gene="typA"
                     /locus_tag="O2692_001959"
     CDS             complement(8073..9920)
                     /gene="typA"
                     /locus_tag="O2692_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCZ4299581.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            10022..10213
                     /locus_tag="O2692_001960"
     CDS             10022..10213
                     /locus_tag="O2692_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HCZ4299582.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(10367..11194)
                     /locus_tag="O2692_001961"
     CDS             complement(10367..11194)
                     /locus_tag="O2692_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCZ4299583.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWIVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPHHDALIQLHEQRFKRKSK"
     gene            11351..11965
                     /locus_tag="O2692_001962"
     CDS             11351..11965
                     /locus_tag="O2692_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HCZ4299584.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLRELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLIEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            12149..>13449
                     /locus_tag="O2692_001963"
     CDS             12149..>13449
                     /locus_tag="O2692_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCZ4299585.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIIL"
BASE COUNT         4336 a         2421 c         1996 g         4696 t
ORIGIN      
        1 taagtagaat catgcatcta aaagagatta atatttaagc ttcaaatttg agtaaacgtg
       61 gattacataa ttatcccaat aaaaaaatca ttacgattaa gttcttttta tgtcgtccac
      121 atacaatact tgtaaaatta aatcatattt cctgcgttgg atcccatctt ttcatatcct
      181 acaatcaggt ctatttatag tatcatctca aatccggcta ttaattctaa ttctcagtga
      241 tgcgtttttt tatgatgggg tgtataaatt gtttggcaaa aagaatttaa aatggttagg
      301 tgtcgtagca acgttaatga tgacatttgt acaacttggt ggagccttag ttaccaaaac
      361 cggatcagct gatggttgtg gttcttcttg gccactatgt catggtgcgt tgattccaga
      421 attctttcct attgatacga ttattgagtt aagtcataga gccgtttcag ctttgtcttt
      481 attaatggtc ttatggttag ttatcactgc atggaaacat ataggctata ttaaagaaat
      541 taaaccttta tcaatcatta gtgttggatt cttattattg caagcattaa tcggagctgc
      601 tgctgttatt tggcaacaaa acgattacgt tttagcattg cactttggta tatcattaat
      661 cagtttctca tctgtatttt taataacatt gattattttc tctatagatc aaaaatatga
      721 agctgacgaa ttatatatca aaaagccatt aagacgttta acatggttaa tggcaatcat
      781 catttattgt ggtgtttata ctggtgcact agtgagacat gcggatgcaa gtttagcata
      841 tggtggttgg ccattgccat tccacgatct tgtaccacat tcagaacaag attgggttca
      901 actcacgcat cgtatcatgg cttttatagt gtttacgatt attatgatta cttatattca
      961 cgctgttaaa aattatccaa ataaccgtac tgttcattat ggttatacag ctgcatttat
     1021 acttgttata ttacaagtta tcacaggtgc attatctatt atgacaaatg ttaacctgat
     1081 aatcgcactt ttccatgcat tatttatcac ttatttattt ggtatgacaa catactttat
     1141 catgctaatg ttacgatcag taagaagtga caagcaataa caaaaaagca aaccgtaatt
     1201 ttaatggcac gcccattaaa attacggttt tttatatcaa tatttaaaat taaacctaag
     1261 ccatgtaaaa acgagattac acgtcaattg ttgtgtaatc tcgttttatt ttaatcattt
     1321 tagtcagttg ctttttcaat ttcgattaat aaatcgcctg tcgctattgt gtcaccatta
     1381 tttacagtta cttgtttaat cacaccgtca aatggtgctt gaattgttgt ttccattttc
     1441 atagcttcag taattagcaa cggctgatta gctttcacag tttcacctac actaaccttg
     1501 acttcagtta ctgaacctgg catttgagca ccgatatgac ttggattact cttatctgct
     1561 tttggcttaa cgttcgcatt tgtatgcaca ttttcatctt taatgtaaat acgtctcgct
     1621 tgaccattca tcgcatagta aatcgttcta ttaccatttt catctggttc actaatcgtt
     1681 tctagtttaa taattaatcg tttaccttta tcgatttcga tttctactgt ttcaccatta
     1741 cgcattccaa agaagaatgt aggcgtatca agtaacgata agtttccgta ttgatttcta
     1801 gtttgaatat attgttcata tacttttgga tatagtacat aactaataat atcttgctcc
     1861 gtaacaggac cttgttgctc ttcttcaagc aactcacgga ctttttcaaa atcaactggc
     1921 tctagatatt cacctggacg agctgttagt gcttcttggc cttttaaaat aaccgcttgt
     1981 aaatctttat taaaaccatt tacaggttgt cctatttcac ctttgaagaa cgacactact
     2041 gattctggga aatctaattt atagccatct gtaatcacgg attgttcatc aagatcattt
     2101 tgtaccatat aaagtgccat atcaccaact actttagacg atggtgttac ttttacgata
     2161 tcaccaaata agaaattcac tctgcgatac atatctttga cttcatcaaa tctttcgcct
     2221 aaacctaaac ttttagcttg ttgacttaaa ttcgaatact gtccaccagg catttcatgt
     2281 tgataaattt cagtattcgg tgatttgata tcactttcaa agtctgaata ataagtacgt
     2341 acagttgacc aataatgact aagtgactcc ataccttcaa tatcagttct aaggtggcgt
     2401 gggaagccat ttaatgcata atataacgaa ttggcgcttg gctgacttgt taaaccactc
     2461 attgaagcaa cagcagtatc aatgatatcg acaccagcat ctattgcttg tttgtatgtt
     2521 aataaaccat taccacttgt atcatgagtg tgaagatgaa ttggtaaatc tacagctgat
     2581 tttaactcac caatcaattc gtaagcggct ttaggtttta ataagcctgc catatcttta
     2641 atcgctaaaa tatggaaacc ttcacgttct aactctttag ctagtttgac ataatactct
     2701 aaagtataaa tgtttgatcg ctcaggattt aaaatgtcac ctgtataaca aatagtacct
     2761 tctgagattt tgcccgcttc ttgtactgct tcattggcaa ctttcatttg atctacccag
     2821 tttaatgaat cgaaaattct aaagacatct atgcctgctt tagcactttc ttgtacgaat
     2881 ttatgaataa cattatcagg atagttttta taaccaactg cgtttgaagc acgtaacaac
     2941 atttggaata atacatttgg aatagcttta cgtagacgtt caagtcgttc ccatgggttt
     3001 tccttcaaga aattatatgc cacatcaaat gtagcaccgc cccacatttc tagtgagaaa
     3061 ccatctttaa atacgtccgc tgttttggat gcgatattaa tcatatcctt agttctaact
     3121 cgtgtagcta ataatgattg gtgtgcatct ctaaaggttg tatctgttag taagacatca
     3181 tcctgctttt taacccattc agctacacct tttggaccta cttcatcaag caattgtttc
     3241 gtaccactaa atgaagcgat tttacttgaa gatacagttg gaattgatgc taattcatag
     3301 tctggtttcg gacgtttctc aacatttggg aaaccattaa ttgttacatt acctatatat
     3361 tctaatgttt tagtacctct atctagagac ggctgaatgt tgaaaagttc tggtgtttct
     3421 tcaataaatt tagttgtgta atcaccactt gtgaactttt tattcttcat tacattaatt
     3481 aagaatggaa tattagtttt aacaccacga atacgcattt ctcgtaatga gcgtaccatt
     3541 ttttcttctg cttgtttaaa tgatatcgcg tgtgtagata attttacgag taatgaatca
     3601 taataaggtg atatctcagc accttggaaa ccatctccag catcaagacg tacaccaaag
     3661 cccccgcttg aacgataagc aatgattgtt ccagtatccg gcatgaaatc atttaacgga
     3721 tcttctgttg taatacgaca ttggatggca tagcctaatg ttgtaatatc tttttgttgc
     3781 ggcatattaa tctcttcacc aaataaatcg gcacctgctg caactaaaat ttgtgtctta
     3841 acaatatcaa ttcctgttac catctctgta attgtatgct ctacttgtac acgagggtta
     3901 acttctataa agaagaattc gtcaccagat actagaaatt caacagtacc tgcattgaca
     3961 tatttaatat tttccatcaa ttgaattgca gcatcacaaa tacgttgacg taatgttgat
     4021 gataatccaa ctgatggtgc aacttctaca actttttgat gacgacgttg tactgaacaa
     4081 tcacgttcaa ataagtgtac gatatttcca tgttcgtcac ctatgacttg tacttcaata
     4141 tgctttggat tatcaatgta tctttctatg taaacttcac tattaccaaa tgatttttca
     4201 gcttctgatt ttgctctatg gaaagcatct tctaattcac tttcttcacg aacgattctc
     4261 atacctttac cgccgccacc acttgtggct ttaatcatta gcgggaaacc agcttcttct
     4321 gcaaattctt ttgctaattc atatgattta attggaccgt ctgtaccagg aataactggt
     4381 aaatctgcct tgatagccgt tgtacgagct ttaactttat ctccaaacat atctaaatgt
     4441 tctaaatgag gaccaataaa tttaattcct tcttctgcac aacgacgcgc aaattgttca
     4501 ttttcactta aaaatccata gccaggatga atcgcatcca cattcgcttg ttttgctaca
     4561 tcaatgatac gctcaatatt taaataactt tcagcaggac ctaaatcact tccaactaaa
     4621 taggattcat ctgctttata tctatgtaat gaacttttgt cttcattcga ataaattgca
     4681 actgtgctga tgtctaattc tgccgccgct ctgaatatac gaattgcaat ttctccacgg
     4741 ttagcaacaa gtaacttttt tatttgtttc aatagcgata cactcctcaa actattagaa
     4801 ttttctaact aattagataa taaaatttta tcttaaagcg ctctgttttg ctatagttat
     4861 gtttcaaatt ttcaaaattt aacattcttg agacaattaa aacctccgct tcagaaataa
     4921 taatttcaaa aatgactatg caacaacagg tagttcacgt ttttgttgtg aaacattttc
     4981 gatttctaca actctaaaaa attaaaaata aaattgcaaa acatcaacat ttattatcaa
     5041 tagcgataac tttatcttat catcatgatt ctaatttcgc caccacattt agtaattttt
     5101 agtcataaaa tttagttata attatacgtt gttttgttta taaaatttga taatcatgag
     5161 taatctcgta atatcaaaac aaaaaggaag ttaagcgttg tttggttgcc taacttccgt
     5221 tattgaactc atcagtccaa tttgacaaag agtatataaa tagccaatgt ttagcattgc
     5281 ttaatgacag aagtcatctc gtactaaata ttggctagta tttttttaat taaattgtct
     5341 tcttatatca actttttgtt gttgtttctt tcgttgctgg tctactttga tttgtttacc
     5401 tacaatcaga agtaaaccca tagcaatact taaactaatc attgatgatc caccaaagct
     5461 gataaatggc aatggcacac cagttaatgg aattgttgcc gaaataccgc caatgtttac
     5521 aaacgtttga cttccaaagt atgtggcaat cccaacacac acaagtttat aaaaatatga
     5581 tgatgtttta tttgcaaact ggaaggcacg atatacaata aagaactcta aagtaataac
     5641 tagcaatcct ccgattaaac ctaattcttc gcaaataatt gcaaaaataa aatctgtatg
     5701 tggttctggt aaatagccca atttcattgc actatttcct aatccttttc caaatacgcc
     5761 accgttacct atcgcaagca atgaattgga aatatggtat ccagttcctg attcgaattg
     5821 gaatggatct gttagcgtac taaatctggc agttaaataa cttggtaacc aaccagccat
     5881 taatgcaatg acaaatacta ctaagaatcc tagcactgct ggtataccaa atcttaggac
     5941 tttgtttacc ccaattcctg aataaaaaat gatcgcaact aaaataatta atattagtaa
     6001 tgtttgccct acgtcttttt gtaggaaaac taaaaacgta caacctaatg ctaatacaat
     6061 aggacttaaa attaattttg gtttacttaa tactctaggc atttttttac tgatcataaa
     6121 tgggatatat aatatgattg caatttttaa taactcagat gcctgtaagt tcataaatcc
     6181 taagtttatc caacttttag aaccattaat atctttacct attactaacg tcagtaataa
     6241 tagtgagacg atagttataa tcataccttt ttgcacttta atattactca gtaatttaac
     6301 attcattaaa aatgcaataa aaaatacaat tataaaactc attatgacat atgctaattg
     6361 tcggttgtaa aaatacgttc ctggaacatc gataccacca gtcaatgtgc ctttagttgc
     6421 tggaaccata cttgcactat ataccatcac taatccaatt aaactcaata caatatatgt
     6481 aactaataac ggataatcaa taaactttga ggttttacca atataccgta aaatacttct
     6541 aaaattcttc atatattcag tcatccaatt ctacaattta tacttcaaat gatttcgttt
     6601 tatactttaa ttttaaaaat ccatgttact tattatatac atgacacagt cataatcaca
     6661 aatattaaca ggtctcttga ggtaatttat agaatatttg tttacttaat atcgtgaatt
     6721 gctaaaaccc aataaaagtg actgacatgt catcaagaag acaacataac agccacttta
     6781 atcagtaatt aaactataaa tataaaatcg gcgcagctca attgtcgata tctcacgcca
     6841 ttttaatatg cttatatatt aaaccttaaa caagtgtaaa agcttcatgt aattttgaaa
     6901 gttctttctc aagacgtaac ataatttgtt tgccatcttc gcggtcaact aaacctaatt
     6961 taacagcaaa atcaacttct ttttgaagtc caaacatttg tgtatctagt acttcttcat
     7021 ataatgggca tgaaggtaat gttaaattat ctagttgaac tttaatcaga tgcaagattt
     7081 catcagcatc tttagtcaat tgtttcaaag ctgcattttt cattgttgct tgtttcgcca
     7141 tgacacgctc cccctctata taaattctca agtcttacta aaagtttatc ttactaaata
     7201 ttaaaaagca agtagattta aaatgtgtcg ttagcaattt gtgttaaaat aatgctatat
     7261 acattgatag ggagtgggaa aatgatacaa attaaaggag ccgtaaagtt tcctataaca
     7321 ttagatagta cgacttggat tttcgatgat agaaaagtta gcatcgaaga tttagaaaaa
     7381 ggtgtatttg atggttcaaa accaatcgac tttgaagata atcatgaatg gaatcgtgct
     7441 atcctagaag gacaaactaa tccgcctact ttaaattcag aaataaaata taaaaaacgt
     7501 gccatgttag aaggttcatt tgtcattaat atgacaccat ttttcaaaca ttccgaacca
     7561 catgactctg caactactat tcgattatca aatgagcatg atcatttaga tataccgata
     7621 gatctattac cctatttatt tttccaattt gcaaaagacg gtaaacgact ttacagcgat
     7681 aatggtgtag atagttttat ttacaatcaa gaagatggtt attcatatcg aatcaaccat
     7741 gtaactcata ttgaggtgat ttaatttgag acaagtgcaa tgcattatct gtgatgcaaa
     7801 agtatttata gatgagcgca caactgagtc taaacgactt aaaaacaatc ctattcgaac
     7861 atttatgtgt gatgattgta aaagtcgatt agacacacct aaacaacgtg cgcaacacta
     7921 tccgctcgat taatatctga ataaacaagt tagaaatttg aaaatgtgaa agtaacagga
     7981 aataatagca tttaaacact ttttcaaata ttatatgcca aaagagaagc taagcaactt
     8041 tcatcgctca gcttctcttt tattatgtta aattattcgt tttcttgcat catttgtttg
     8101 atacgctttg cttctttttc acgaacattt ttgtttaaaa ttttctttct taaacgtata
     8161 ctttctggtg taacctcaac aagttcatca tcattaatga attgtaacgc ttcttccaat
     8221 gttagaatac gcggtctatt cattgtttgt gtttggtctt tcgttgcaga acgtacgtta
     8281 gtttgatgtt ttgttttagt gatgttaaca gttaaatcat tttcacgatt atgttcacca
     8341 acaatcatac cttcataaac ttcagtacca ggttccatga agtttacacc tctatcttca
     8401 agtcccaaaa tggcataagt acttgcagaa ccttgatcca ttgaaattaa tgcaccatta
     8461 cgacgaccgc caatttgtgc tttaatacgt ggtctaaatt cttcaaatgt atggttaata
     8521 ataccgtaac ctcttgtcat tgacataaat tcagtcgtat aaccaatcat accacgagcc
     8581 ggtacattaa agattaaacg tgtaagtcca ttatcagttg tagtcatatc aaccatttca
     8641 cctttacgtg cacctaatga ttcaataaca gcacctgcat tttcttgtgg cacttcacat
     8701 tgcacacgtt caaatggttc acacattaca ccatctattt ctttaataat tacttgtggt
     8761 tttgaaactt gtaattcata accttcacga cgcatatttt caataaggat tgataaatgc
     8821 aattcaccgc gaccagcaac tacccatgta tctggagaat ctgtgttaga aactttcaaa
     8881 gatacatctg tttctaattg ttgatttaaa cgttcttgaa tttgacgtgc tgttacaaag
     8941 tcaccttcac ggccagcaaa tggagaattg ttaactttaa atgtcatttc aagagtaggc
     9001 tcatcaatac gtagaactgg caatgcttct tgatggtcat gtggtgttac agtttcacca
     9061 acattaatgt cttccatacc tgaaacagca attaaatctc cagcttgtgc ttcttcaatt
     9121 tctaaacgtt ttaatccaaa gtaaccaaag attttagtta cacggaagtt tttcactgta
     9181 ccgtctaatt taattagtga tacattatct ccgacacgca ttttacctct gaatacacga
     9241 ccaataccaa tacgtccaac ataatcattg tagtccaaca atgctacttg gaattgtaat
     9301 ggctcatcac tgttatcaat tggagctggt acataatcaa taattgtttc atataatgat
     9361 tgtaaattat catcttgctt ttcaggatct aagctagctg taccatttac tgctgaagca
     9421 taaacaacag ggaattctaa ttgttcatcg tttgcttcta attcaataaa taaatctaaa
     9481 acttcatcta caacaccctc tggacgtgct gatggtttat caattttatt aacaacaaca
     9541 acaggtttca ggttttgttc tagcgctttt ttaagtacaa aacgtgtttg aggcattgta
     9601 ccttcatacg catctactac taagacaacc ccatcaacca ttttcataat acgttctact
     9661 tctccaccaa agtctgcatg tcctggtgta tccaaaatat taatacgtgt acctttataa
     9721 tcaacagccg tatttttggc tagaatcgta attccacgct ctctttcgat atcgttagag
     9781 tccattgcac gttcatcgac atgttcattt tctctgaata taccagattg ttttaacaac
     9841 tcatctacta aagttgtttt accatggtca acgtgagcaa taattgctat attgcggaca
     9901 tcttctcttt tattagtcat ataaatagtt tccttctttc ttaagtaaaa tcccactttt
     9961 aattacaact tttttattat atcatataat tgagaaaaga taagcaaaag gtggggatag
    10021 gatgaaacaa aaaaaatcta aaaatatctt ttgggtattt tctatattag ctgttgtttt
    10081 cttagtatta tttagttttg ctgttggtgc atcaaatgta ccaatgatga ttttaacatt
    10141 tatattactc gttgcaacct ttggaattgg atttactaca aagaaaaaat atcgagaaaa
    10201 cgattggcta taagttatat cgacaataac acgcgttgat ttaagaccac acatgttcat
    10261 gatgaacatt taactggttt tatattggcg cgtgttttta ttatttaagg tataaaagcg
    10321 taatatgtta cctaattgtt aggactacat attacgcttt tggtaattat tttgattttc
    10381 ttttaaatcg ttgttcatgt aattgtatta acgcatcatg gtggggctct aaataatcat
    10441 tgctaatttc ttgatggaga ccacgatttc caactaagat tgaatttgga ccactaattg
    10501 ttaatggttc tcctagtaaa ttggaagctt gtccatttac ttcatataaa ataaccaatc
    10561 cgccagcaaa atcccatggt tgaagtcttg gcgtcatata tgcttctaaa ttacctgtag
    10621 caactgaaac gatttcaagc gctgcactac catatgccct tgcacttcta gaatcattaa
    10681 caatttcttt aaagatttct cctaaaattg gtttagttaa ccagttcgga ttgatcccaa
    10741 taatgctttg tcttagatta gaatcattca atggtttcaa gggttggcta ccacgatatg
    10801 caccttcccc tactttagca tgatataaga catcagccat aacatcatat acaaaacctg
    10861 cataaggttt accatcgata taaataccaa ttgaaattgc gaaattttct tgttgatgaa
    10921 caaaattcaa tgtaccgtct attgggtcaa caatccatac cgtacctttg gaagtatcga
    10981 tgtcatgacc atgcccttct tcacctaata cttgatgatt gggatatgtt tctaaaattg
    11041 tatcaaaaat gaaatcttct gttgctttat ctacatttgt aacaaggtca ttcggatttg
    11101 actttgtttc aattgttaaa ttttgctcca tcaattgttt aattctaatt cctgcttctt
    11161 gaataagtcc ttgggcaaat ccataaagtg ccataacatc atctcctaac atctttatta
    11221 tacatcaaca ttttataaaa ataacttctt atgataaaaa tgaaaatact aattttaatg
    11281 agagcgtctt aagccgcaat tgataaaaca tatgctacaa tatttttaaa cactatacag
    11341 gaggtactct atgacaaaat atacatttaa acctaaagat ttcaaagcgt tcaacgtaga
    11401 aggcttagac gcacgaatgg aagctttaaa cgaatacata cgaccacaac tccgtgaatt
    11461 aggagaatat tttagtgact tctttacaag tcaaactggt gaaacatttt atcctcacgt
    11521 agcaaagcat gctagaagaa gtgtgaatcc tcctaaagat acatgggttg cttttgcaac
    11581 aaacaaaaga ggctataaaa tgttacctca tttccaaatt ggtatgtttg aagatcaact
    11641 gtttgttatg tttggaatca tgcatgaagc aaaagataaa gcaacacgtg caaaagtttt
    11701 tgaaagaaaa tttaaagcta ttcaacaatt acctgatgat tatcgtgttt gcttagatca
    11761 tatgaaacct gataaaccat ttattaaaga tttaacggat gatgatttaa tagaagcgat
    11821 acaaagagcc atcaatgtga aaaaaggtga attctttata gcgcgtgcaa tcacaccaca
    11881 agataaaaga ttaaaaagtg acaaagcatt tattgcattt ttagaagaaa ccttcgatca
    11941 gttcttacca ttttattctg cataaataac tttgtttaaa taatagagca cttaatcaca
    12001 tccatgattt cgtgctcttt tttcttaata ttaaatcgaa cgttcaacat aataattcat
    12061 acttttaaaa aaattaaaat aaatttaggt tgacctaaac attttattag gttattatat
    12121 tgtccataag aagtagaggt gagtcaaaat gaataataaa cgacattcaa caaatgaaca
    12181 attaagttta gacgaaataa acaatacaat taaattcgat catcgcagtt caaataaaca
    12241 gaaattttta tcatttcttg gacctgggtt attagtcgct gttggttaca tggatcccgg
    12301 aaactggata acatcaatgc aaggtggcgc ccaatatggc tatactttgc tattcgtaat
    12361 tcttatttca agtttatcag caatgttact tcaaagcatg acagtgagat taggaatagc
    12421 aacaggtatg gacttagctc aaatgacacg acattattta tcaagaccta ttgctataat
    12481 cttttggatc attgcagaac tagcaattat cgctacagat attgctgagg ttattggtag
    12541 tgctattgct cttaatctcc tatttaacat acctttaatc gtcggtgcac taataactgt
    12601 acttgatgta tttttactac tttttataat gaaatatggt tttagaaaaa ttgaagctat
    12661 tgttggtaca ttaattttca cagtgttatt catctttata tttgaagtct atatttcatc
    12721 accacagttg aatgctgtgt taaatggatt tataccacat agtgaaatca ttacaaataa
    12781 cggcattctc tatattgcat taggtattat tggcgctaca attatgcctc ataatttgta
    12841 cttacattca tcaattgtac aatctagaac atactcaaga cataacaatg aagaaaaagc
    12901 gcaagcgatt aaatttgcta cgatagattc gaacattcag ttatcaatcg catttgtagt
    12961 caattgctta ttattagtgt taggagcatc actatttttc aactcaaatg ctgacgattt
    13021 aggtggtttc tatgatttat atcacgcctt aaaaactgaa cctgtactag gtgcaacaat
    13081 gggtgcaatc atgagtacat tatttgcagt tgcattatta gcgtcaggtc aaaattcaac
    13141 gattactggt actttagcag gacaaattgt aatggaagga tttttaagat tacacatacc
    13201 aaattggtta agacgtttaa ttacacgttc tcttgctgtc attcctgtta tcgtatgctt
    13261 aatcattttt aaaggtaatg cagctaaaat tgagcaacta cttgtattct ctcaagtatt
    13321 tttaagtatt gctctgccat tctgtttaat accattacaa ttggctacta gtaataaaga
    13381 cttgatggga cctttctata ataaaacatg ggttaatatc atttcatgga cacttattat
    13441 tattctaag
//
LOCUS       DALAGF010000075        13256 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.075, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000075 DALAGF010000000
VERSION     DALAGF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13256
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.075"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            108..308
                     /locus_tag="O2692_001964"
     CDS             108..308
                     /locus_tag="O2692_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299586.1"
                     /translation="MTNPTRTKRCGGDVLYGIDNHFQLLLYISENHCQLETKTFFEYF
                     LRIVNKTHDYAILENKKICISH"
     gene            385..516
                     /locus_tag="O2692_001965"
     CDS             385..516
                     /locus_tag="O2692_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299587.1"
                     /translation="MLLPSSKSHIEMMCEMFKLRSFCENKDNNYSFYNSEKSVYDNN"
     gene            778..1437
                     /gene="arlR"
                     /locus_tag="O2692_001966"
     CDS             778..1437
                     /gene="arlR"
                     /locus_tag="O2692_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HCZ4299588.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            1434..2789
                     /gene="arlS"
                     /locus_tag="O2692_001967"
     CDS             1434..2789
                     /gene="arlS"
                     /locus_tag="O2692_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HCZ4299589.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            3073..5871
                     /locus_tag="O2692_001968"
     CDS             3073..5871
                     /locus_tag="O2692_001968"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCZ4299590.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRTKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5885..7153
                     /gene="sucB"
                     /locus_tag="O2692_001969"
     CDS             5885..7153
                     /gene="sucB"
                     /locus_tag="O2692_001969"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCZ4299591.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
     gene            7733..8542
                     /locus_tag="O2692_001970"
     CDS             7733..8542
                     /locus_tag="O2692_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCZ4299592.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
     gene            8571..8774
                     /locus_tag="O2692_001971"
     CDS             8571..8774
                     /locus_tag="O2692_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299593.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            8955..9746
                     /locus_tag="O2692_001972"
     CDS             8955..9746
                     /locus_tag="O2692_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCZ4299594.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQGDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            9760..11646
                     /locus_tag="O2692_001973"
     CDS             9760..11646
                     /locus_tag="O2692_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HCZ4299595.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDILVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDI
                     LKEDMLNEYYYLPKTLYNTLASPEFDDLKRTDASQVDGQDDTSEDDDNESEKADSKSA
                     DSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGD
                     AVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIER
                     EQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKM
                     AETKKGVVLFHETLKALNIKHEILSFSEDAFDSDEHAQPNIINEIINYDYSTFEKDGP
                     RIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEA
                     VEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLL
                     LKSL"
     gene            11871..13214
                     /gene="brnQ"
                     /locus_tag="O2692_001974"
     CDS             11871..13214
                     /gene="brnQ"
                     /locus_tag="O2692_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCZ4299596.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
BASE COUNT         4919 a         1880 c         2342 g         4115 t
ORIGIN      
        1 aaacaaaagc agtaaaccta aagtgtcgta agggtttact gcttttataa aacgttgtta
       61 taacgtatat tgtcttttac gggcatataa aaggggaata tttgaaaatg accaatccaa
      121 caagaacgaa acgttgtggg ggggatgttc tatatggtat tgataatcat tttcaactac
      181 tattatacat tagtgagaat cattgtcaat tagaaactaa aacttttttt gaatattttt
      241 taagaatagt aaataaaacg catgattacg ctattttaga aaataaaaaa atttgtattt
      301 ctcattagaa ttagaatatt taaaagtgat gaggtttaaa cattatattg tttacatact
      361 ccttttgaat tcatacatta tgaaatgtta cttccaagtt caaaatcgca cattgaaatg
      421 atgtgtgaaa tgtttaaact acggtcattt tgtgaaaata aagataataa ctattcattt
      481 tacaatagtg aaaagtcagt atatgacaac aattaatatt gcggtaaggc cttgtgttac
      541 agtattctat atttaagtac tgcaatcaga attaacagaa tgccattaac tgattattaa
      601 atatttgagt taataaataa ttaatgattg tagcttgaaa aatttaaaac atggttattg
      661 atttgtgata aaatttaaac gtaaacaaac taatttaaaa agcaactatt gtatagaaaa
      721 atacaaaatt taaaatatat taccttatta gaaaaagtcg taatatgagg tgtacaaatg
      781 acgcaaattt taatagtaga agatgaacaa aacttagcaa gatttcttga attggaactc
      841 acacatgaaa attacaatgt ggacacagag tatgatggac aagacggttt agataaagcg
      901 cttagccatt actatgattt aatcatatta gatttaatgt tgccgtcaat taatggctta
      961 gaaatttgtc gcaaaattag acaacaacaa tctacaccta tcattataat tacagcgaaa
     1021 agtgatacgt atgacaaagt tgctgggctt gattacggtg cagacgatta tatagttaag
     1081 ccgtttgata ttgaagaact tttagcaaga attcgtgcaa ttttacgtcg tcagccacaa
     1141 aaggatatta tcgatgtcaa cggtattaca attgataaga acgcttttaa agtgacggta
     1201 aatggcgcag aaattgaatt aacaaaaaca gagtatgatt tactatatct tctagctgaa
     1261 aataaaaacc atgttatgca acgggaacaa attttaaatc atgtatgggg ttataatagt
     1321 gaagtagaaa caaatgtcgt agatgtttat ataagatatt tacgaaacaa gttaaaacca
     1381 tacgatcgtg acaaaatgat tgaaacagtt cgtggcgttg ggtatgtgat acgatgacaa
     1441 aacgtaaatt gcgcaataac tggattattg ttaccacgat gattacgttt gtcacgatat
     1501 ttttgttttg tttaattatt atttttttct tgaaagatac actgcataat agtgagcttg
     1561 atgatgcaga acgaagctca agcgatatta ataatttatt tcattctaag cctgttaaag
     1621 atatatctgc attagacttg aatgcatctt taggtaattt tcaagagata attatttatg
     1681 atgagcataa taataaatta tttgagacat cgaatgataa cacagtgaga gttgaaccag
     1741 gttatgaaca ccgttatttt gaccgcgtaa taaaaaaacg ctataaaggc attgaatatt
     1801 taattattaa agaaccaatt acaacgcaag atttcaaagg gtatagcttg ttaattcatt
     1861 cactagaaaa ttatgataac atcgtaaaat cattgtatat cattgcgctg gcatttggag
     1921 tgattgcaac aattataact gccacaatca gttatgtatt ttcaacacaa attactaaac
     1981 cgcttgtcag tttatcaaat aaaatgattg agattcgacg agatggtttt caaaataaat
     2041 tgcaattaaa tacaaattat gaagaaatag ataatttagc aaatacgttt aatgagatga
     2101 tgagccaaat tgaagaatca tttaatcaac aaagacaatt tgttgaagat gcgtcacatg
     2161 aattacgaac accattacaa attattcaag gtcatttaaa tttgattcag cgatggggaa
     2221 aaaaagaccc agcagtatta gaagaatcgt taaatatttc tattgaagaa atgaatcgta
     2281 tcataaaatt agtcgaagaa ttacttgaat tgactaaagg agatgtaaat gacatttctt
     2341 ctgaagcaca gaccgtgcat attaatgatg aaattcgctc gcgaatacac tcattaaaac
     2401 aattgcatcc tgattatcaa tttgatacgg atctgacatc taaaaatcta gaaattaaaa
     2461 tgaaacctca tcaattcgaa caattatttt taatctttat tgataatgca atcaaatatg
     2521 atgtgaagaa taagaaaatt aaagttaaga caaggttaaa aaataagcaa aaaataattg
     2581 aaattacaga tcatggaatt ggtattccag aggaagatca agatttcatt tttgatcgct
     2641 tttatcgagt ggataaatct cgttcaagaa gtcaaggcgg taatggactc ggattatcta
     2701 ttgctcaaaa aatcattcaa ttaaacggag gatcgattaa aattaaaagt gaaattaaca
     2761 aaggaacaac gtttaaaatc atattttaat catgactgag acgtcaatca aagtcatagg
     2821 atcaattttt taagtacaca ttagctgtga ctaatgtata agaacaacta taaaacaaat
     2881 aaacagtggt tctttatcat ttctgttgta ctcccaaaat ttacaataaa atacatctat
     2941 aaacctagaa gaatcaacgc ttttgttgat tcttcttttt agcagataaa taggtaaatc
     3001 tactttaaca aataactaaa tagtgatatt attacattgt aagcgtttca acatttttgt
     3061 ggagggtgta aaatgactaa cgaaagaaaa gaagtttcag aggctcctgt aaacttcggt
     3121 gcgaatttag gtctaatgtt agatctatat gatgactttt tacaagatcc atcatctgta
     3181 ccagaagatt tacaagtctt attcagcaca attaagaatg atgactcaat tgtaccagct
     3241 ttaaaaagta caagtagtca aaatagcgac ggcacaatta agcgtgtcat gcgtttaatt
     3301 gataatattc gccaatacgg gcatcttaaa gccgatattt atcctgtaaa tcctccaaaa
     3361 aggaaacatg tacctaaatt agagattgaa gactttgatt tagatcaaca gactttggaa
     3421 ggtatatcag caggaattgt ttcagatcac tttgccgaca tttatgataa tgcttatgaa
     3481 gcaattttaa gaatggaaaa acgttacaaa ggaccaattg catttgagta tacacatatt
     3541 aataacaata ccgaacgtgg ttggttaaaa agaagaattg aaacgccata taaagtaacg
     3601 ttaaataata acgaaaaaag ggcactattc aaacaattag cgtatgttga agggtttgaa
     3661 aaatatcttc ataaaaactt cgttggtgca aagcgttttt caattgaagg ggtagacgca
     3721 cttgtaccga tgttacaacg tactattacg attgctgcga aagaaggtat taaaaatata
     3781 caaataggca tggctcaccg tggacgttta aacgttttaa cgcatgtctt agaaaaaccg
     3841 tacgaaatga tgatttcaga atttatgcat acagatccaa tgaaattctt acctgaagat
     3901 ggtagcttgc agttaactgc tggatggact ggtgatgtga aatatcacct tggtggcatt
     3961 aaaactactg attcatacgg tacaatgcag cgtattgcac tggctaacaa tccaagtcac
     4021 ttggaaattg ttgcacctgt tgttgagggg cgtacgagag cagcacaaga tgatacacaa
     4081 cgagctgggg ctccgacgac tgatcatcat aaagcaatgc caattattat acatggcgat
     4141 gctgcttatc ctggtcaagg aattaacttc gaaacaatga acttaggaaa cttgaaaggc
     4201 tattctacgg gtggttcatt gcatattatt actaacaata gaattggatt tactacagaa
     4261 ccaattgatg cacgttcaac aacttattct acagatgtgg ccaaaggtta tgatgtgcca
     4321 atattccatg tcaatgcaga tgacgttgaa gctactattg aagcaattga tattgcaatg
     4381 gaatttagaa aagagtttca taaagacgtc gttattgatt tagtaggtta tcgtcgtttc
     4441 ggacataacg aaatggatga accatcaatt actaatccag ttccttatca gaatattcgc
     4501 aaacatgact ctgttgaata tgtgtttggt aaaaagcttg ttaatgaagg tgtcatttca
     4561 gaagatgaaa tgcattcatt tatagaacaa gtccaaaagg aactaagaca agctcatgat
     4621 aaaattaata aagctgataa aatggataat ccagatatgg aaaagcctgc agatcttgca
     4681 ttaccgttac aagcagacga acaatcattt acttttgatc acttgaaaga aataaatgat
     4741 gcattgttaa catatccgga tggctttaac attttgaaaa agttaaacaa agttcttgag
     4801 aagcgtcatg agccgtttaa taaagaagat ggtttagttg attgggcaca agcagaacaa
     4861 cttgcatttg cgacaatttt acaagatggt acaccgattc gcttaactgg tcaagatagt
     4921 gaacgtggta cattcagtca taggcatgcc gtgttacatg atgagcaaac aggtgaaaca
     4981 tatacacctt tacatcatgt tcctgatcaa aaagcgacat ttgatataca caattctccg
     5041 ctttcagaag cagcagtagt tggttttgaa tacggctata atgtggaaaa caaaaaaagc
     5101 ttcaatattt gggaagcaca atatggtgat tttgcaaata tgtcacaaat gatttttgac
     5161 aacttcttat tcagttctcg cacaaaatgg ggagaacgtt caggattaac attattctta
     5221 cctcatgcat atgagggtca agggcctgaa cattcatcag caagattaga gcgattttta
     5281 caattagctg ctgaaaataa ttgcacagtt gtcaacttat ctagttcaag taattatttc
     5341 cacttattgc gtgcacaagc ggctagttta gattctgaac aaatgcgacc attggttgtt
     5401 atgtcaccaa aaagcttact gagaaataaa acagttgcaa aaccaattga tgaatttact
     5461 tctggtggat ttgagccaat tttgacagaa tcatatcaag cggataaggt tacaaaagtt
     5521 attttggcaa ctggtaaaat gttcattgat ttaaaagaag cattagctaa aaatccagac
     5581 gaatcagtat tactcgttgc gattgaaaga ttgtatccat tcccagagga agagattgaa
     5641 gcattactag cacaattgcc aaaccttgaa gaagtgtcat gggtacaaga agaacctaaa
     5701 aatcaaggtg catggttata tgtctatcca tatgttaaag tgctagttgc agataaatat
     5761 gatttaagtt atcatggcag aattcaaagg gctgctccag ctgaaggcga tggagaaatt
     5821 cataaacttg ttcaaaataa aattatagaa aatgcattaa aaaataacta gggggaaata
     5881 agtcatgcca gaggttaaag ttccagaatt agcagaatct attacagaag gtaccattgc
     5941 agaatggttg aaaaacgtag gggatagcgt agaaaaaggt gaagctattc ttgaattaga
     6001 aactgataaa gttaatgtcg aagttgtatc tgaagaagca ggtgtattat ctgaacaact
     6061 tgcaagtgaa ggcgacactg tagaagttgg acaagcaatt gctatcatcg gcgaaggtag
     6121 tggcaatgct tctaaagaaa atagtaacga caatactcca caacaaaatg aagaaacaaa
     6181 taataaaaaa gaagaaacaa caaataattc ggtagataaa gctgaagtaa atcaagcaaa
     6241 tgatgacaat cagcaacgta ttaatgctac gccttctgcg cgtcgatatg ctcgtgaaaa
     6301 tggtgtgaat cttgctgaag taagtccgaa aacaaatgat gtggttcgta aagaagatat
     6361 tgataagaaa caacaggcac cggcatcaac acaaacaaca caacaagcat ctgcaaaaga
     6421 agagaaaaaa tacaatcaat atcctacaaa accagtgatt cgtgaaaaaa tgtcacgtag
     6481 aaagaaaaca gctgccaaaa aattattaga ggtatctaat aatacagcta tgttaacaac
     6541 atttaacgaa gttgacatga caaatgttat ggaattgcgt aaacgtaaga aagaacaatt
     6601 tatgaaagat catgatggta ctaaattagg atttatgtca ttctttacta aagcttctgt
     6661 agcagctttg aaaaagtatc cagaagttaa tgcagaaatc gacggcgacg acatgattac
     6721 gaaacaatat tatgatattg gtgtagctgt ttctacagat gatggattat tagtaccatt
     6781 tgtaagagat tgtgataaaa agaattttgc agaaatcgaa gcagaaattg ctaatttagc
     6841 agttaaagca cgagagaaaa aacttggctt agatgatatg gttaatggtt catttacgat
     6901 tacaaatggc ggtatttttg gatcaatgat gagtacgcca attatcaatg gtaatcaagc
     6961 tgcaatctta ggcatgcatt caattattac aagaccaatt gcgattgatc aagatacaat
     7021 cgaaaatcgt ccaatgatgt atattgcatt aagctatgat catagaatta ttgacggtaa
     7081 agaagcagtt ggattcttaa aaacaattaa agaattaatt gaaaacccag aagacttatt
     7141 attagaatct taatccacaa cacaaaatag tttactattc ctaaaagcgg gattaaatca
     7201 ataacaaaca gcagtaagat tatttcctag tcgaaatatc ttactgctgt actttatttt
     7261 tataatgatt cgtattaaaa tgtgggttta attttgacta cttaaaaaat atatcatttc
     7321 tattgaaata gactcacaat agaaatatag gtagaatagc gtgtttcaat atgctaatga
     7381 atgcatttta gataataaca aacgatgaaa tatatgaatt ctataagcgc ctttagttaa
     7441 tttaaaatca tctgaacatg atgtaaattc gctttatgga cacgacatat atatgttttt
     7501 catgtcctac aaaaaacgaa atattcaatt gctatataca gatattcatt aatgacacac
     7561 tcaatagcaa ccataacaat attatgttac ctatttaaat gaaatagttc ttttaaagaa
     7621 atacattttt cacatattaa tctataatca aaatcaactg accgatattc tataatttat
     7681 gattaaaata agttataata taatagtaaa gataaagata gaggtggcta taatgtgcgg
     7741 acttagaagt ataacattag gtacaacaaa tatagaacag acaaaacatt tcatggttga
     7801 catattagga ttaaattatg aagaacttct tgaaaactca attcgtttcg gcgatgcaga
     7861 tataagccca ggaacaagac ttcaatttat acaagttcca agtgagcaat tagaagaatc
     7921 tcactttgtg ggtattggat tacgtacacc aactgactca ggtttagagg agtatgcgga
     7981 aatattatcg aataaggata ttccatttac aacagttaaa gaattaaatg gcaataaata
     8041 tttcagtctc gaagataaca atggtcatat tttctcaata tattcaaacg agaataatta
     8101 tggcgttggt ttaggtatgc cttcttttga gagtgcggtc aatccgttac atcaagtgca
     8161 aggtttagga ccagtgattc ttaaagtgaa tcatgtagat attacaggtc aaattttaac
     8221 aaatatattc ggacttgaag tatttgcaga ataccaaccc ttcgacaatg ctgactatca
     8281 tgtccaagta ttcaaagttg gaacgggtgg tctaggtggc gaaatacatt taatgcctgt
     8341 agaaaccgaa atgacaatgc cggaatatgg cgcagtcgat caagttgagt ttgaaacgaa
     8401 agatgcagat ttctttaatc aagcgaaatc gcgcttagat gaagtggaaa taccatatca
     8461 aacgcttgag caagatgata ttgaatcaat tagaattact gaaaacagtg gattatcgtt
     8521 tatattcact ttacaaaaat aattttttta cgatagcgag gacaaaattt atgttacatg
     8581 aaacttggaa agaacgtaca ccaatcaaga aagtagaagt cattaataca gatgcaaaga
     8641 aatttacagt ttctgacatg ttaacggttg gcaaacaata tgatgttatc aacgaaactg
     8701 aagaatatta tcaaataata gataattctg gattagttgg cggttactat aaaacatatt
     8761 tcaaagaagt ttaaacgcat aattattgaa atgaagccta atatgagatt ttcaattagt
     8821 aaatttggta aaatataaac aattcgcata ctaagcattg cgttttatat cagaatagaa
     8881 tagtaaacta aggcatttgg agaacttatt ccatgtctta gttttttaat ttatttaaag
     8941 gagaacgttt taaaatggca ctaaaacatt ataagaattc agattcaaca gttttcaatg
     9001 atgcgaaggc attatttgat ttaaataaaa atattttact taaaggtcca acaggttcag
     9061 ggaaaacaaa gttggcagaa acattaagtg aagttgttga tacacccatg catcaagtca
     9121 attgttctgt tgatttagat acagaaagct tattaggctt taaaacaatt aaaacaaatg
     9181 cggaaggtca acaagaaatt gtctttgtag atggtccagt tattaaagct atgaaagagg
     9241 ggcatatttt atatattgat gaaataaata tggctaaacc tgaaacattg cctgtattaa
     9301 atggggtctt agattatcgt cgtcaaatta cgaatccata cactggtgaa gtaatcaaag
     9361 ctgtaccagg atttaacgtt atagcagcga taaatgaagg ttatgttggt actttgccaa
     9421 tgaatgaagc actaaaaaat cgctttgttg ttattcacgt tgattatatt gatggggaca
     9481 ttttaaaaaa tgtgattaaa gagcaaagtt tattacaagg tgataaacaa atcgaacaaa
     9541 ttattaagtt taatgaagat ttacgtacta tgtctaagca gggacaaatt tctgaagaag
     9601 ccgctagtat ccgtgcatta ttagacttgt gtgatttaat cactgtaatg ccagttgaac
     9661 gtgcaattaa acgtacaatt attgataaat tggaagatga acgtgaacaa caagcaatat
     9721 ataatgctgt agaactaaac ttttaaagag ggataaaaca tgagtgatcg tttcataaaa
     9781 tttaatgacg aacagttaga tgccaagcaa gtaatgatgt tgcaagactt agctcgactc
     9841 ttattaaaaa atgaacaaac acaagttaaa atccaaaaat tcccttacta taatcctgtt
     9901 caaaatgttt tgattacgag ttggttttgg tcacatcgac caagccatat cgaaatggct
     9961 ggtttaaaaa cagatgttat gttagctgct tacggttatc atatgatgga tgtacaaatt
    10021 gtaaatgaag tagtacaaga taaaactttt aaacacccta aattttatca acaacttttt
    10081 aaattattag aagatatgcg cgtattaaat tctattaaag ttgagcgccc aagtactgct
    10141 aaattaattg atcttcgctt agatacacgt atatcatata cggaatctca aattaaagtg
    10201 tatcgtacta aaacacaata tacagattta ttatttttgt atttagaaca tgcattttta
    10261 agccaagatt tctttgatat accatctata cattcagatt tagatgatat tttagtaaat
    10321 atgttcttat atttacctaa ttttttccaa aatcaaaatt ctgaagataa tatgtatctg
    10381 gctcaaagaa ttatgtatca agtagatgat attttaaaag aagatatgct aaacgaatat
    10441 tattacttac ctaaaacact atataataca ttagctagtc cagagtttga tgatttaaag
    10501 agaactgatg caagtcaagt cgatgggcaa gatgatactt cagaagatga tgataacgaa
    10561 agtgaaaaag ctgactctaa atctgcagat agtgagtcaa aaggtggcgc ctatttagaa
    10621 atggagcttc acgaaggaca aaatagtgaa acattaggta atgatgaagc acgtgaaggc
    10681 gatgctacag atgatatgac tgatatgatg accaaaaagg gcaaaggatc aaacgatacg
    10741 ctaaatcgtg aagaaggcga tgctgttggt caaagccaag catttcaact tgatggtgtg
    10801 aacaaaaatg tagaaataaa atggcaaatt cctgaaattg agccacaata tgttttagaa
    10861 tatcaagaat cgaaacaaga cgtacaatat gaaatcaaag acttaattca aattataaag
    10921 aaaacgattg aacgagaaca acgagatgca cgttttaatt taacaaaagg gcgattacaa
    10981 aaggatttaa ttaattggtt tatcgacgat caatataaat tgttttacaa aaaacaagat
    11041 ttaagtaaat catttgatgc gacatttaca ttgttaattg atgcgtcagc aagtatgcat
    11101 gataaaatgg ctgaaacgaa gaaaggtgta gtgttattcc acgagacgtt aaaagcattg
    11161 aacattaaac atgaaatttt atcattcagt gaggatgcct ttgattcaga cgagcatgca
    11221 caaccaaata tcattaatga aattattaac tatgattact caacctttga aaaagatggc
    11281 ccacgtatta tggcacttga acctcaagat gataatcgtg atggtgttgc tattagagtt
    11341 gcaagtgaac gattaatgcg tcgaaatcaa catcaacgat ttttaatcgt attttcagac
    11401 ggcgaaccgt ctgcatttaa ttatagtcaa gatggtatta ttgatacgta tgaagctgta
    11461 gaaatgtcac gtaaatttgg tattgaagta tttaatgtat ttttgagtca agatccaata
    11521 actgaagatg ttgaacaaac aattcataat atttatggtc aatatgctat tttcgttgaa
    11581 ggtgttgcgc atttacctgg acatctctct ccattattaa aaaaattact acttaaatct
    11641 ttataaataa atttaaaata tgagtttata tgaaattgtg aataaaagtc atcagttact
    11701 atttaaaaag tggatttgaa gaattttatt ttctgacata atttgattta aacaacacat
    11761 gtgttttgtt tctgctatgt acaacatatt tcatatagac tctttattat tttttgtgct
    11821 aaaatattta cttgtaaatt atttcgtgtt ttaaaaaaag gagtaactct atgaataaaa
    11881 atacatgggt cattgggttt acgctcttcg ctatgttttt tggcgcaggc aatctaattt
    11941 ttcctcctaa tctcggattg gatagtggtc aatttttctg gcccgccatt ttagcgtttg
    12001 ttctaactgg gattggttta ccattattag gtgtgattgt aggtgcactt gataaagaag
    12061 gatatattgg cgcattaaat aaaatttcac ctaaattttc aatattgttc ttaatcatca
    12121 tttatttgac tataggacca ctttttgcaa tacctagaac tgcatctaca tcttttgaaa
    12181 tgacaattac accaattata catagcaata gtagtatcgc tttatttata tttacgatta
    12241 tctacttcat agtcgttttg tatatttgtt taaatccatc taagttaatc gatcgtattg
    12301 gttcattatt aacaccatta ttattgatta ctattttagc gatgattatt aaaggatact
    12361 tagactttag cggtaatagt gctggaaagg gcaatgaagc actatatcat tctaattttt
    12421 caagttttgc tgaaggcttt acacaaggct atttaacaat ggatgccatt gcagcaattg
    12481 ctttttcaat gattgttgtt aatgcagtaa aactaacagg cattactaaa acaaatcaaa
    12541 tattcaaaca aactttgact gctggtttaa ttgcagccgt agctttaatt ttcatatata
    12601 tttcattagg ttatattggt aatcatatgc cagtaagtga catgacgtta gatcaattga
    12661 aatccaaaga tcgaaacatt gggacatatt tattaacgac aatggcttca acaggatttg
    12721 gttcattcgg aaaatattta ttgggcatca ttgtggcgct ggcatgtcta actacagcat
    12781 gcgggcttat tgttgcagtt tctgaatatt tccatagaat cgtacctaaa gtatcataca
    12841 aagcatttgt attagttttc attttaatga gttttattat tgctaaccaa ggtttaaatg
    12901 ctgttatctc aatgtcaatt ccggtattaa gcattgtata cccagtagca ataactgttg
    12961 tattattaat tttaattgcc aaattcatac cgacaaaacg catttcacaa caaattccag
    13021 ttattatcgt atttatattg tcgattttca gtgttattag taagttaggt tggctgaaaa
    13081 ttaactttat agaatcattg cctctaagag cgtattcttt agagtggttc ccagtagcaa
    13141 ttattgcaac gatattaggc tatctagtcg gcatatttgt aaaacaagat ccaattaaat
    13201 atcaacagga ataacgaata atataaaaga ggttgggaca taaatcccta aaaaaa
//
LOCUS       DALAGF010000076        13236 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.076, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000076 DALAGF010000000
VERSION     DALAGF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13236
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.076"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(143..556)
                     /locus_tag="O2692_001975"
     CDS             complement(143..556)
                     /locus_tag="O2692_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299597.1"
                     /translation="MKPTKVILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDCDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVAKSDFTKFINELASLH"
     gene            complement(585..695)
                     /locus_tag="O2692_001976"
     CDS             complement(585..695)
                     /locus_tag="O2692_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299598.1"
                     /translation="MDGNNQIDVCFFVLVNNYYTMSIYPRIINSNGDYAT"
     gene            complement(781..1512)
                     /locus_tag="O2692_001977"
     CDS             complement(781..1512)
                     /locus_tag="O2692_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCZ4299599.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            complement(1514..2827)
                     /gene="murT"
                     /locus_tag="O2692_001978"
     CDS             complement(1514..2827)
                     /gene="murT"
                     /locus_tag="O2692_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HCZ4299600.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            3119..3619
                     /gene="ftnA"
                     /locus_tag="O2692_001979"
     CDS             3119..3619
                     /gene="ftnA"
                     /locus_tag="O2692_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HCZ4299601.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            complement(3701..3793)
                     /locus_tag="O2692_001980"
     CDS             complement(3701..3793)
                     /locus_tag="O2692_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299602.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            4008..4562
                     /locus_tag="O2692_001981"
     CDS             4008..4562
                     /locus_tag="O2692_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCZ4299603.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKIHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            complement(4630..5700)
                     /gene="dinB"
                     /locus_tag="O2692_001982"
     CDS             complement(4630..5700)
                     /gene="dinB"
                     /locus_tag="O2692_001982"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCZ4299604.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(5947..6477)
                     /locus_tag="O2692_001983"
     CDS             complement(5947..6477)
                     /locus_tag="O2692_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCZ4299605.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            complement(6647..8008)
                     /gene="rlmD"
                     /locus_tag="O2692_001984"
     CDS             complement(6647..8008)
                     /gene="rlmD"
                     /locus_tag="O2692_001984"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCZ4299606.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPLAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            complement(8089..9036)
                     /locus_tag="O2692_001985"
     CDS             complement(8089..9036)
                     /locus_tag="O2692_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCZ4299607.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
     gene            complement(9619..9765)
                     /locus_tag="O2692_001986"
     CDS             complement(9619..9765)
                     /locus_tag="O2692_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299608.1"
                     /translation="MIADLDAIEGLFISIEVKLLLTLFIVHVNGKNINFINALALIIL
                     RQTV"
     gene            complement(9805..11232)
                     /gene="gatB"
                     /locus_tag="O2692_001987"
     CDS             complement(9805..11232)
                     /gene="gatB"
                     /locus_tag="O2692_001987"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCZ4299609.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            complement(11245..12702)
                     /gene="gatA"
                     /locus_tag="O2692_001988"
     CDS             complement(11245..12702)
                     /gene="gatA"
                     /locus_tag="O2692_001988"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCZ4299610.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            complement(12704..13006)
                     /gene="gatC"
                     /locus_tag="O2692_001989"
     CDS             complement(12704..13006)
                     /gene="gatC"
                     /locus_tag="O2692_001989"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCZ4299611.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
BASE COUNT         4163 a         2432 c         1836 g         4805 t
ORIGIN      
        1 cccgcctctt tttatcgtat atagcatgtc cctttttaaa attacaagca taaatattca
       61 gactcaaata tagccacacg cctaaactac ggagggatgt ggctgtcttt tttgattgta
      121 attacatatc agatttaaat cattaatgta aagaggctaa ttcattaata aatttagtaa
      181 aatctgattt agcaacattt atccttaatg aaataccaga ttctgttgcc atgtttgaat
      241 gccttaaacc agaatcaaaa tcaatataaa tacaaagcag ttcttgatta aaatcaataa
      301 ttgtaaaatg taaatcaggt tctataaaat atatttcagt aacaggattg tcgcaatcca
      361 tatgtttcaa attgtccatg tcactatcaa agagtgcaaa ttcaaattta acttcttgat
      421 cttcgaaagt tgcactagtg tcgaaataat aaattttatt atcttcttcg ataactacat
      481 cttttaaaat aaatgttatc gatgttttgc tatgtaaata agatgcatct tttaatatca
      541 ctttagtagg cttcatcgtc aaattccttt catcattcat tttttcatgt tgcataatca
      601 ccattactat tgataattct aggatagata ctcattgtat aataattatt cactagaaca
      661 aaaaaacaca catcaatttg gttgtttcca tccaaaataa tgtgtgtgtt tatacatata
      721 tgattaatta ctaaacttca tcgtttatca atattaaatc cattgatgcg atgttcagag
      781 ttaacgagat ttcttctgtc tatttgctct gtctattaat acttgtttcg cttgtatttc
      841 cgcttcatta tctatttctt taggctcaaa cggaataccc ttacgttcac aagctttttc
      901 taacagataa tcagtgattt cgtaattttt aggtaaaatt ggtccatgta aataagtacc
      961 taataaattt ttataatgaa tgccttcttt tttatcttca tcattattac cataaccaaa
     1021 agtaacatga ccaagtgtac cgaaatcatg atatgttcta ccaccgtgat tttcaaaacc
     1081 tacaatagtt ccaaaagtat cactttcgat aacaatatct cctgttaatc ggtttgtctt
     1141 tgattcagta taaaaatcta aaatacctaa cccttctaat tctgtaccat caggcgtgat
     1201 atattttttc cctaaaaatt gatagcctcc acaaatcgtt aatcccggca taccatcttc
     1261 aatcgcttct ttaagtggtg tcttaatttt acttaattct tttgttgcta atgcttgttc
     1321 tctatcactt ccaccaccga taaagaaaat atcacattca tcaaaggtaa taccttctgt
     1381 ttcattgatt tctacgacat taactttaat atttcgtttt ttagcacgtt gtcttaaagc
     1441 aataatattt cctatatcac tgtataaatt caatttatct gacataaaat gataaatagt
     1501 caattcatgc atattatgat tgacctcctt caaacgaacg gtttaattgt tcaagcatag
     1561 gcgctaatga tgtatagttt ggtattgcaa ctgtgaaacc tttataatcc atagtctttg
     1621 ccgttgcttt ataaatatca cgctcaacta taattggtac ttcaacctct gctaacttca
     1681 atcgcaattg aagttcttct gctcgtgtac ctgtcacgat gatagcttca atttgttgct
     1741 tagataattt ttcaaaatct gcatcataaa tccatgaagt atctcgacca tctgcagcgt
     1801 tatcatttag cgaaataaca tacacttttt cgccttctaa ttgttcacca actgataaac
     1861 ttgcattcat tcctgcagga tttttagcta aattgatcat cgcttctttt cgttcttttt
     1921 taaagtactg catacgacca ttgtctgatg tatacgtttc aaagccattt ttaattgttt
     1981 gttcatttaa ccctagctct cttaaaacag tatatgctgc taacgcgtta taagcgttaa
     2041 agtcacctgc aattttcata tcatattttt catcattgat atttaaatat aaaaacggtg
     2101 ccacatcaaa acttgatatt tcatattttg cttgctctcg tttgaaacca cactgacagt
     2161 gataatgacc aatttgatta taatgaatat aatcgtattg caataagcga ccacagtttg
     2221 gacaatatct actttcattc atcgtacttt gttcaaattc atgggcatgt gctttcatac
     2281 catagtacac aatcgtatca cttgcgattt tcaaacgact cacaaatgga tcatcagcat
     2341 ttagcaataa tttgatgcct ttattactaa ttgtctctgc aatgttatta accataatat
     2401 caatttcacc gaagcgatcc atttgatctc taaagaaatt agtaaatacc atcattgaag
     2461 gtgtaacttc ttttaacaca cgtggaatcg aaccttcatc aatttcgatt accgcaattt
     2521 tagtcttagg tgttgattgc atgatgaatg cagaagttat acctgcagcc atattagcac
     2581 cttcattatt gtgtataatt tgaatattat ttgcttttaa agtatgtcca attaagtttg
     2641 aagtcgttgt tttaccattt gttccactga taaatacaat atcatcaact tgctctgcta
     2701 attttcttaa tatatctgta tccacttttc tagcgatttg tccaggtaaa tctgttcctc
     2761 ttttacctac tgctctactt gctttacgcg ccaatttcgc tagatggatt gccgtccact
     2821 gtctcatgtg tttcctcctc aaatttccac tcgcatcatt ataacatgac aaggcaactt
     2881 caaaaaagtt tctcaatcac aaattgatac agtgtttcta aattataatc attctcattt
     2941 agataattaa aaatgatatt tattctcaat taaatatcgc taaatgatac attttctcaa
     3001 ttagagtgct aaaataaggc attaaattat aattattatt atttacaatt gagaatgagt
     3061 ttgttatact aagtttaatt catagtaatt ttaatttaca aataagaggt gtatcaaaat
     3121 gttaagtaaa aatttattag aagcattaaa tgatcaaatg aaccatgagt actttgcagc
     3181 acacgcatat atggcaatgg cagcatactg tgataaagaa tcgtacgaag gatttgcaaa
     3241 cttcttcatt caacaagcta aagaagaacg tttccatgga caaaagattt ataactatat
     3301 taacgacaga ggtgcacatg cagaattcag agcagtttca gcaccaaaaa ttgacttttc
     3361 aagcatacta gaaactttca aagacagctt atctcaagaa caagaagtaa caagacgttt
     3421 ctataactta tctgaaatcg ctcgtcaaga taaagattat gcaactatct cattcttaaa
     3481 ctggttctta gatgaacaag tcgaagaaga atcaatgttt gaaactcaca tcaattattt
     3541 aactcgtatc ggcgatgaca gcaatgcatt atatctttac gaaaaagaac ttggcgctcg
     3601 tacattcgac gaagaataat taaacatcac tacaatagac agataaatat catacgacat
     3661 gataggcatt tgggtcactt acaataaccc aatgtctata ttattttgct ttacggagat
     3721 cactagattc attttctgaa tcattgatct gcgttttttc attttcaagg ctaattattg
     3781 tatttttagt catttatttt ttaaactact aatgttaata actctaaatt tgatgttgaa
     3841 ttaatttgac gattttaaag catatcatca tttacttttt aatcagagtt acatccaaat
     3901 gatagatttc acgttatacc ttcacgtata atattatgta tcgtttgtaa gcaaatgact
     3961 aaaagtctat taatatatac atttaattaa ttgaaaggat tgactacatg atacaagatg
     4021 cgtttgttgc acttgatttt gaaacagcaa atggtaaacg tacaagtatt tgttctgtcg
     4081 gaatggttaa agtcattgat agtcaaataa cagaaacatt tcatactctt gtgaatccgc
     4141 aagactattt ttcacaacaa aatattaaaa ttcatggcat acaaccagaa gatgttgaaa
     4201 atgcacctac gtttgactac gtatttccat atatgatgca atttattgca gatttacctg
     4261 ttgtcgcaca taacgcggca tttgatatga acgtcttaca tcaaagcatt caaaatattg
     4321 gtttaccaac tccaaattta acttactttt gtagttatca acttgctaaa agaaccgttg
     4381 attcgtatcg atacggttta aaacatatga tggagtttta tcaattagat tttcatggtc
     4441 atcatgatgc attgaatgat gccaaagcat gcgcaatgat tacttttagg ctactgaaaa
     4501 attatgaaaa tttaacatat gtaactaata tttatggtaa aaatctaaaa gataaaggct
     4561 aggactaaat aaaatactcc cttcaaaagt aagcattgta aaaatgtaaa ctttgcaggg
     4621 agctttattt tatataaagt catatatcgt catattttta taagttgatt gttctaaatt
     4681 acctacagtg acaccaataa gtcgaattgg tacatcaggg tcttttaaat cgttataaag
     4741 taaatatgca atattataaa tatcttcttc agaactaacc gaatctctta aactcatctg
     4801 tttagatagc gtttcaaatt gataagtttt aattttaacc gttacagttt tagctgactt
     4861 ctgtaattta tttagacgtt cagctgtttt acctgacaat tcccatactt ttcttaaaat
     4921 ctcttcatca tcattcacgt ctgttgcaaa tgtgcgttca gtccctactg attttcttac
     4981 tcttgatgat ttcacttcac tatggtcaat accgcgtgcc ttgttatata aaccccgacc
     5041 tctttttcca aacaaacgta ttaattcaaa ttccgttttc tcatataaat ctctaccgtt
     5101 aaaaatacca ttatcatgca ttactttttt ggaagcttta cctacgcctg gaaaatctcc
     5161 aatatccaat gtcatcaaaa tatcatggac attttgataa tcaatcacag tcataccatc
     5221 aggtttattc ataccactcg ctaatttagc taaaaatttg ttataagaaa cacctgcaga
     5281 tgctgttaaa tgtgtctgct ctagaatatc ttttctaata tactgagcaa ttttcgaagc
     5341 aggaaggtct ggtctcacta attctgtaat atctaaatac gcttcatcca atgacatcgg
     5401 ttctacctta tctgtataac ttcggaaaat agacataatc tgcgcagatg tttctcggta
     5461 agcaccaaaa ttacttgtga caaagtatcc atttggacat aatttatgcg cttgtgacat
     5521 aggcattgct gaatggacgc cgtattttcg tgcttcatag gatgccgtag agacaacacc
     5581 cctactgctt gctttaccac caacaatgac tggtttccct ttcaatttgg ggttatctct
     5641 catttcgact tgtgcaaaaa aatagtccat atctatatga ataattcgtc tctcagtcaa
     5701 gtgctcacct ccctactaat ttttactttt ataacgcaca aaaatatctc aacataatta
     5761 tacgctgtgt acgatttttt tacataaatc ttgcacttag cgataactat attgagataa
     5821 ctacaagttg ttataaaatc aattgctatt taagcatgat gatgaagacg attgagtaag
     5881 aaaacatagg taatctgaaa taattcaagc aaattcattt tgttggtatc atcatattaa
     5941 aatttattat tgagtcggct tttgatgata caaataaata ctatcttcat cttgttgaac
     6001 atatttaaaa ctactagata ttgttaatgc aacaagcaga atatctataa agcaataagc
     6061 ggtatgcata ctaaacataa aaataagtga tgaataacca aataccttaa ttaaaataag
     6121 caagccagta cttaatagga ttagtggtga cagcataata attgagaatt gccatttgtt
     6181 gaagcaagca tctgctgttt ggaataagat tctgtctttt tttatattaa acataggttt
     6241 gctatctttt ttaaataaaa gaaataatgc tctatggata agttcatgta aaatcaataa
     6301 aataatgaat ccagcaaacc catatacaag attgatgatg atattttgat cgacaaccgc
     6361 tgtgacacct aacgcccact tatacgtaaa taaaatcacg aataacgcaa taacaagttg
     6421 caagataata aaccttcgca tttgaaaatt atttgtcgtt aaatcaattt tatgcattac
     6481 caaccctccc gatcatgaca ttcttattct tctttaaata tagtatacaa tgtcacattt
     6541 aatttaaaaa gttcatatca agaaagtaaa ttggctgtaa taaaatttta atatacgact
     6601 tctttcttca cttattaagg cgaaatttta tctcaaatca tgtgcgctat ttcaaattga
     6661 ataatgccac tgtctcaaca tgtgttgttt gtggaaacat atctaccggt gttacctctt
     6721 caagttgata tttttcagct aataataatg catcacgttg ctgtgttgcg ggattacatg
     6781 aaatatagac aatacgctta ggttctaatg taagcaaagt ctgaataaac gtttcgtcac
     6841 agccctttct tggcggatca accattacaa catctggttt aatcccttgt gctttccatt
     6901 gtaaaataac ttcttcagct ttcccacaga caaaagttgt attattgcat tggtttatag
     6961 tcgcattttg ttgtgcgtct tcaattgcag aaggtactac ttcaacaccg tatacatgtt
     7021 ttgcaagtgg tgccatatat agccctattg ttccaatacc acaataggta tctaatacaa
     7081 cttcattacc tgtcaattgc gcatactcaa ttgctttatt atataatttc tctgtttgtt
     7141 cagaattaat ttggtagaat gactgatcac ttattttaaa tgtactatct gttaattgat
     7201 caataattgt atctttacca tatagcgtta tagattgacg tcccataata acattagagt
     7261 ggctatcatt aatgttttgt ttaatgcttg tcacattagg aaatgcatct aatatcttct
     7321 caacaacagc atttttttgt ggccactttt taccattagt tacaaaaata atcatcattt
     7381 cgtctgtatg atatcctgtt cttacaacca aatgtctcat taaacctttt ttcaattgtt
     7441 cttgataaat acttacattt aaatctttta aaatagattt aacttcattc atcacttctt
     7501 gatgttgtga atcttgtatt aaacaacttt ccatgtcaat aatgtcatgg cttctttgac
     7561 gataaaagcc cataataact tcattctgtt cattcttacc aactggaatc tgggacttgt
     7621 ttcgatatct ccaaggatct gtcatgccaa ctgtatcgtt aatcttagaa ttatcaaaat
     7681 gcgcttttcg ctgaaacaaa ttaatcactt gttccttttt catttcaagt tgtgcttcgt
     7741 atgataagtg ttgaagttgg cacccaccac aacgttcata atatatacat ggcggtgcaa
     7801 cacgattctc acttctcgta tttatattaa gcaacttacc tatcgcaaag ttctttttta
     7861 ctttgataat tttatattca atttgttcat taattaaagc ttgtggtatg aaaataggaa
     7921 agcgatctat ttttacgaca ccatggcctt catgcgttaa atcaacaact gttcccgttt
     7981 ttatgtcatt tttagctatt gcttgcaaaa ttttacctcc aaaatgaaca ggttaggaac
     8041 aaaattatgc gcttcctaac ctgccattat atatttcact atttctgttt attcttcgat
     8101 taaattgtca tcaacatgat cattatttat taactcttca tttacaatat cattaggtgc
     8161 aaagacatct atatgacgtt ctagatttaa gaaattcgct ggtaatttac caccatattc
     8221 tccatctaca tttagttgta agtctgtgaa tgatgaaata ttaattgcct ttgctttttc
     8281 ataaataact ttaggatgct tagtatgttc tcctcttgaa gctaaagtca taatatgacc
     8341 aagttctgca aggtttgatt tttcaactat aattaacgta aaatagccgt catctaactt
     8401 agcgtccggc actaattttt caaatcctgc cattgaattt gttaaaccta aaaagaataa
     8461 taatgcttct ccttggaaaa cattaccatc atattcaatt cttaaatcta cagctttcat
     8521 ttgaggtaac atttcgaaac ctttgatgta ataagcaaat ggaccaacaa tagatttcaa
     8581 tttactcggt gtttcataag agacttgcgt caattgtccg cctgcagcta aattaataaa
     8641 gtatcgatta ttcattttac caatatctac tttagtagaa tgaccttcaa tgatgacatc
     8701 aagtgccccc atgatgtcat taggtatatg caatgcacgt ccaaagtcat taacagtacc
     8761 cataggaatg acacctagct taggacgatt aggcttttct gcgataccat taactacttc
     8821 atttaatgtt ccatcaccac ctgcagcgat taatacatca taattttcat gcatagctct
     8881 ttctgcttca agtgtggcat cacctatttt ctcggttgca tatgcactcg tttcatatcc
     8941 cgctttttct aattttatta aggcatcagg taattctctt ttaaatagct ctttacctga
     9001 tgtcgggtta taaatgattc tagcacgttt cctcatatct tatccctcta cttaaaattc
     9061 atatatttta acttcatctt tgtttcgtct aatagggagt gggacagaaa taatatttaa
     9121 caaaatttat ttcgttctac cccaacttgc attgtctgta gaatttcctt tcgaaattct
     9181 ctatgttggg gccccacccc aacttgcaca ttattgtaag ctgacagaaa gtcagcttct
     9241 ttgtttgggg gccccgccaa cttgcacatt attgtaagct gacagaaagt cagcttctat
     9301 gttggggccc cactagaatt gaaaaaagct tgttacaagc gtattttctt tcagtcaact
     9361 acagccaata taacattgta gtgcctagga cattgaattt atgacccagg ctcagtctta
     9421 tttcatcatt cttaatatcg ttaaagacca acttgtatct taaacaaata ctatctcaat
     9481 atgtacaaag cttgttattt attcagcatt ttttgccgtt cttcattata tagacttcgt
     9541 cagttatgct attttacctt taaaatgatg ttgtaaatat aatgttgtct ttatacaaaa
     9601 atgttttata cctttttatt atacagtttg ccttaatata attaaagcta acgcattaat
     9661 aaaattaata tttttaccat taacatgtac aatgaataaa gttaaaagta atttgacttc
     9721 tatagatata aataaaccct cgattgcatc taagtcagca atcaagggtt tattttttaa
     9781 atcttcatag tttgatgatt taaattatct tttatctaat tcttgtttta atagttgatt
     9841 tactaattgt ggattagctt gacctttaga cgctttcata atttgaccaa ctaagaagcc
     9901 catagctttg cctttaccat ttttgtaatc ttcaactgat tgttcgttat tgtctaatgc
     9961 ttcatttaca aattttagaa gtgttgcttc atcagaaatt tgaactaagc cattatcttc
    10021 cataatctgt ttagcattac cacctttagc tgctaactct gggaagactt tcttcgcaat
    10081 tttactgctc attgttccgt cttcgataag tttaatcata cctgctaaat tttctggtgt
    10141 taatttagta tctaataatt ctacttgatt tttatttaaa tattcgttta cgccacccat
    10201 taaccagtta gatgttaatt taacatctgc accgtgttca attgttgatt caaagaaatc
    10261 tgacatttct ttagtcaatg ttaatacgtg tgcatcgtat gcaggtaaac ctaattcatt
    10321 tacatactta gctttacgtt catctggtaa ttcaggaatt gtctgacgaa cacgctcttt
    10381 ccaagcatca tcaatatata aaggtacaat gtcaggctct gggaagtaac ggtaatcatc
    10441 agaaccttct ttaacacgca ttaaaattgt tttacctgta gattcatcaa atcgacgtgt
    10501 ttcttgtccg atttctccac catttaacaa ttcttcttct tggcgttttt cttcatattc
    10561 taaaccttta cgtacatagt taaatgagtt taagtttttc aattcggctt tagtaccaaa
    10621 tttttcttga ccatatggac gtaaagagat gttagcatca caacgtaaag atccctcttc
    10681 catcttaacg tctgatacac cagtgtattg aataattgaa cgcaattttt ctaaatatgc
    10741 atatgcttct ttaggtgaac gaatatctgg ttcagatacg atttcaatta gcggtgtacc
    10801 ttgacggttc aagtcaacta atgaatactc acctttatgt gttgacttac cagcatcttc
    10861 ttccatgtga agacgagtaa taccgattcg ttttgtttca ccgtcgactt cgatatcgat
    10921 atatccattt tcaccaattg gttgatcaaa ttgagaaatt tgatatgctt ttggattatc
    10981 tggatagaaa tagttcttac ggtcaaactt agattctgtt gcgatttcca tatttagtgc
    11041 cattgcagca cgcattgccc agtctactgc acgcttatta acaactggta agacacctgg
    11101 atatgctaag tcgataacat ttgtatttga gttaggttct gctccaaaat gcgctggtga
    11161 tggagaaaac atttttgagt ccgtttttaa ctctacgtga acttcaagtc ctataactgt
    11221 ttcaaaatgc atgatttcca ctccttataa tttttcataa acgtcatgta aattgtattg
    11281 tgtttcatat tgataagcga cacgatataa cgttttttca tcgaatggtt taccaatgaa
    11341 ctgtaaaccg attggtcggc catttgattg tccacaagga acagaaatac caggtaatcc
    11401 agctaagttt actggtgttg ttaataaatc attggcatac attgttaatg gatcatcaat
    11461 ttcttcacct aaattaaacg cagttgtagg cgctgttgga ccaactacta catcataatt
    11521 ttcgaatact ttatcaaagt catttttaat caatgttcta actttttgag attttttata
    11581 gtaagcatca tagtaacctg aacttaatgc aaatgtacct aagaaaatac gacgttttac
    11641 ttctttaccg aaaccttcag atcttgacat tttatataat tcttctaatg aatgagcttc
    11701 tttagaatga taaccataac gaattccgtc aaaacgagaa aggtttgacg aagcttctga
    11761 tgatgcaatc acgtaatatg atggaatacc aaatttagta tttggcaatg atacttcctc
    11821 aacgacagca cctaaagatt ttaaagtttc tacagcgttt tgaactgctt cttttacgtc
    11881 atcagctaca ccttcaccta agtattcttt aggtaatgca acttttaatc ctttaatatc
    11941 tttaccaatt tcagatgtaa agtctacatc atcaactggt gcacttgtag agtcattaac
    12001 atctgcacca gaaatagctt ctaatacgat tgcattatct tttacatttc gagtcaatgg
    12061 accaatttgg tctaatgaag atgcaaaagc aactaatcca aatcgagata cacgaccgta
    12121 tgttggtttc ataccgacaa cgccacaata tgcagccggt tgtctaattg aaccacctgt
    12181 gtctgaacct aagctaaatg gtactaagcc agctgcaact gctgctgcag atccacctga
    12241 tgaaccacct ggcactgctt tatggtcaaa tgggttaact gtttttttga aataagatgt
    12301 ttctgttgaa ccacccattg caaactcatc catatttaat ttaccgatta aaacggcatt
    12361 ttcattatgt agtttttcca ttacagtaga ttcgtaaatt ggcacaaaac cttctaacat
    12421 tttacttgca catgttgttt ctaatccgtt tgtaataatg ttatctttta tacccattgg
    12481 aataccaaat aatttgccat ccatttgatc ttttgcttgt aattcatcca attcttgcgc
    12541 ttttttgatt gcattttctt tatccagcgc tagaaaagac ttaattgttg gatcagtctc
    12601 ttcaattgca tcatatatat ctttaacaac atcagatggt ttgatttttt tgtcttttat
    12661 taaagttaat aaattctcaa ccgattcgta gcgaatgctc atcttacgcg tcctcctcat
    12721 tcatgattgt aggcacttta aattgtccat cttctgtttc tttggcattt ttcaaagcta
    12781 attcttgtgg aataccttta attgctttat cttcacgtaa aacgttttgt aaatctaaaa
    12841 cgtgatatgt aggttcaacg ccttctgtat cagcgctatc attttgtttt gcaaaatcta
    12901 aaatgctttc taatgtgttg gccatttctt ccgtttcttc aggagaaatt tgaagtcttg
    12961 caagattcgc gatatgctca acttcttcac gtgttacttt tgtcattaat aaaagcctcc
    13021 tttaagtcat tcatcactaa attgtatcaa atttccaatt aaaaatctaa gtatttatga
    13081 ggtgctactt taatttcata taaactgtat aaacattatc attcgtttat caaatcattt
    13141 tttatgaaaa caacactctt ttaatattag acaacccaat tcaatattat gattatgcaa
    13201 ttttaattat aaaaatttgc tcatatccaa aaaata
//
LOCUS       DALAGF010000077        13104 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.077, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000077 DALAGF010000000
VERSION     DALAGF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13104
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.077"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(102..431)
                     /gene="sspC"
                     /locus_tag="O2692_001990"
     CDS             complement(102..431)
                     /gene="sspC"
                     /locus_tag="O2692_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HCZ4299612.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(469..1650)
                     /gene="sspB"
                     /locus_tag="O2692_001991"
     CDS             complement(469..1650)
                     /gene="sspB"
                     /locus_tag="O2692_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HCZ4299613.1"
                     /translation="MNSSCKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(1732..2742)
                     /gene="sspA"
                     /locus_tag="O2692_001992"
     CDS             complement(1732..2742)
                     /gene="sspA"
                     /locus_tag="O2692_001992"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HCZ4299614.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQS
                     SKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPD
                     AA"
     gene            complement(3270..4424)
                     /locus_tag="O2692_001993"
     CDS             complement(3270..4424)
                     /locus_tag="O2692_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCZ4299615.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPLFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
     gene            complement(4620..5630)
                     /locus_tag="O2692_001994"
     CDS             complement(4620..5630)
                     /locus_tag="O2692_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCZ4299616.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIVYVIHPINADWLLGSSP
                     YTSLENEGQSIFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFK
                     DS"
     gene            5783..6202
                     /locus_tag="O2692_001995"
     CDS             5783..6202
                     /locus_tag="O2692_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ4299617.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDIKSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            6229..6339
                     /locus_tag="O2692_001996"
     CDS             6229..6339
                     /locus_tag="O2692_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299618.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(6409..10179)
                     /locus_tag="O2692_001997"
     CDS             complement(6409..10179)
                     /locus_tag="O2692_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCZ4299619.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKS
                     AAKPAALETQYKTAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYK
                     PQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTING
                     EISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASF
                     ARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSH
                     NYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGV
                     AQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVK
                     EGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQI
                     DKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINA
                     KNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVI
                     YNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIY
                     LFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNN
                     TGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNV
                     QNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVY
                     LYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYS
                     LKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVA
                     QIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISID
                     KINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNK
                     VVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQ
                     NTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(10407..10841)
                     /locus_tag="O2692_001998"
     CDS             complement(10407..10841)
                     /locus_tag="O2692_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4299620.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(10996..11466)
                     /locus_tag="O2692_001999"
     CDS             complement(10996..11466)
                     /locus_tag="O2692_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HCZ4299621.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(11514..12731)
                     /locus_tag="O2692_002000"
     CDS             complement(11514..12731)
                     /locus_tag="O2692_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HCZ4299622.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDNSLLRKEQNDTTDKDKTSNENSDSTNNSDSSNQQQPATDQNSNQNQGGTQQ
                     APQASNNQNGVVN"
BASE COUNT         4079 a         1995 c         2139 g         4891 t
ORIGIN      
        1 aaagaataaa tgttaaagat tattattaca atgaaaaaac gagctggtga gaaattgaat
       61 tctcgccaac ccgctttatg aagtgaaatc atttaaatta gttatactaa gcgctcataa
      121 acgattggtc gcgaagtgcc aataccttgt ttggatgacg tttgcatgag gatatgctgt
      181 gtatcatcat aatcaagaat ataaatgatt tcttcattat caatagatga aaatttaatg
      241 cgttgatgtg ccgtatctat aaaaagaata tgatattgat tgtgacttgt atcatcgcca
      301 tgacgtatac aaattgattt ttgtaattga tgattactcc aattaccaat gaataaattg
      361 atattggttt gttctagatg tgtgagtttg gttgtgtcgt aaactaaatt tataaattgt
      421 agttgataca tagttttacc tgcctttaat caatatctat attacttttt agtaacctat
      481 cattgaacca taccagttat aatcacgatt gaatgataaa tgtaataggc ttgaatctgc
      541 atcttggatt gataattctg tatcccaagg attccagtaa ataagttttt cttggtcatt
      601 aattttagca ttaccaacaa ctgctagcgc atgtcctaaa tgtgggtcat tagggttttg
      661 agatacactt tgtgcaagga tcataattcc tacattatct tttgtaagtt gatcaacttg
      721 ttcatatgat ggtacgcctt cttgataatg aatatctctg ccttgtgatt taccgtattc
      781 aatcatttga ttagggaatg ttgcacaatt aggaaggtct tgctcactta cttcagggta
      841 taatgtacgc ataatatcat gtgcattata agtgtctgta tttttagttg catttaataa
      901 tgctgccata ctgaatcctg cacaccatga gttatcgaat tgttgttctc taattttgaa
      961 gttttttaat gtattttcat attgaacttg atcttcttgt atcgcttcgt tttcttcaac
     1021 tttagtaggt gttactgtat tttttaactc ttgtttcaat tttgctgaaa ctgttttagc
     1081 actttctttt tcttttacat taccaggtag aggcgtagct tttactaatc taactttgcc
     1141 atcttcttca aaataaaacc ctttttcatc agtaagaaca gtgatatttg aatttttatc
     1201 tttaatttgg tctaaatctt tagcgatgaa gtttgaaatt ttaacactgt aattcatgtc
     1261 ttcttttgat ttgtttaaat catctttatt tttaggacta agtgttaaag tataaacaat
     1321 tttaccgtct tttataactg gataatagta actattatct tcttcaccat taaatttata
     1381 aattttaaaa gcttcgccaa gttcatattt accagttttt gcattacttt gtttatctaa
     1441 tgctttagca taaccagcaa attgttgttg tgctagatct tttacttttt gaggtacttt
     1501 gtcactctta acattaattt ctagctgttt agagtgtgaa tcggctttgg ctttattgtt
     1561 attagcaaat gcgcctagtg ataaaataag cattgaaacc attatgatgc ttataatatt
     1621 gaatactcta gatttacatg aactattcat ataataccct ccatttaaat caaaatattt
     1681 tacgttgcta agactttgct tctattttat ctagacgaac ttaattgtta attaagctgc
     1741 atctggattg tctgaattat tgttatcgcc attgtctgga ttatcagggt tgttagggtt
     1801 gtcagggtta tttggttcat ctgggttgtt agggttatca ggattgttag ggttatcagg
     1861 attatctggg ttattaggtt ggtcatcgtt ggcaaaatgg atatcttcaa tattttgttt
     1921 taagaagttg cgtacatttt cattaataaa taccgcacca ttaaattcat ttggtacacc
     1981 gccccaatga attccgatca cttcattttt ttcattaaat acaggtgaac ctgaattacc
     2041 accagttgta cttaaatcat attgcatagc ttcgcctttg aggtaagtga tttttccttt
     2101 actttcccac attgttgcta caggtttatc accaggatat cctgttacag taatattttg
     2161 gttaacttgt gtttcagcat tattactcat tgttgctggt ttaactactt caccaatatg
     2221 tttgttttgc tcattagggg agaatttaac tattgctaaa tcaccttcgc ctgaatattt
     2281 agtgatttgt tcagcagtga aaccaccatt tggataattg tcttggttaa ttgcagaagg
     2341 gaatgctttt aaagcatgag gatcaccgtg cgtagcatct acgacgtgtt tatttgttaa
     2401 aagagtatct ttacctacaa ctacaccgga agcaataaat gtaccagtag gtgcttcaac
     2461 ttgaatataa gttacgggtg cataatgacc attcgttgta tctgtgattt ggtgacgatc
     2521 gttatttggt aatataacat ttgcgtgttc acgttgttct aatggtttaa ggttaccgcc
     2581 tttttgaatc ttaggtgttt gctgtttgct tgactgcgtt tgttgtggat gattgtccat
     2641 agcctttgaa gataacgcgt ttgctgctgg agaactcaca agtgtcgctg ttgtcaaagt
     2701 tgcaacgaat aaagaactaa cttttaaaaa tttacctttc atctaaaaac ctccaaaaaa
     2761 tttatttaca agttaaatat aacactaaaa atttttaagt caataagaat atataaataa
     2821 attaaaaagt ttttgtaaag attagagttt acaaaaacat taagggaatt aaaattgaat
     2881 tgtattatac tttattcaat tgagttaaat tgataagatc gtcaattgtt tgcatttatc
     2941 taaaagaata aataagaaag gaattgagag cgaatggaaa gtatttgttt ataataataa
     3001 atagtaactt ttgaataata attgtttggc caaaggtaga ttaatatgaa tggtgatata
     3061 ttatttgagt gcttaagttt cgaagaacaa ttataagtta gcatattaaa accttttgat
     3121 agaatcattt tgagtgaggt gtttaaagga gaggaatata actcggtagt atttacagtc
     3181 tctatagagt attatttaaa acttatagaa tataaaaaac tgaacgtact atgaatttag
     3241 ttcacgacgt ccagttcaac atatcatttt tatttagtat ttaaatattt agcaagtctc
     3301 ttcatacctt cttttaaaac atccatttca taagcatagg aaatccttac aaatccttta
     3361 ccgaattctg taaaggatga gcctggaacg attgctaaat gtgttgattc aagtaaatcg
     3421 acacaaaatt cgaaatcgtc atcggtgata tgtttaatac ttgggaaaat ataaaacgcg
     3481 ccttcaggtt gagcggtaat ctcaaaacct aatttagtta attcagatac taaataattt
     3541 cttcgttcta cataagcttc gttcatatat ttaggagctt ctaatccttc gttaagtgct
     3601 gttatacatg ctatttgagc tggaacattg gcacaaatac aattataggc gtgcataaat
     3661 gttaatttat caatcaaata ttgaggtcct aatagaaaac caattcttat tccggttgct
     3721 gagtgtgatt tacttaaacc accaattaat attaattgat cacgaatgtc ttcaaattca
     3781 gcgaaggata catgtttacc actaaatgta ttttcagcat aaatctcatc gctaataata
     3841 aataacggat atttttttaa tacatttacg atatttaaaa cttcatttct ttttaaaact
     3901 acgccagttg gattagtcgg ataatttaac aagacagctt ttgtctttgg agaaatatga
     3961 ctttctaatg catcaggtgt aattttaaat tgtgttgctg ttgtatcaat ataaattggt
     4021 ttaccaccta gtacttcgat gagtggtatg tagcctgcat aaattggtcc cggtattata
     4081 atttcatctc caggctctat gatagaacgt aacgttgtgt ctattgcttc acttgctcca
     4141 tttgtcacaa taatttcttc aggatcatag gaaaaatgat aacgattttt gaagtattga
     4201 ctaattgctt cgcgagtttc taataaccct ttattgtgag agtatgatgt cttgtcattg
     4261 ttaatagcat caatatatgc ctttttcaca acatcaggca tcgggaagtc tggttggcca
     4321 atagttaaat taacacaatc atccaaatta ttcatacgat ttgaaaattg gcgaatactt
     4381 ggtgctctta aatatttaga attagaattt aaagaaagtt tcatttgtga cacctcaaat
     4441 ataaatcaaa tattgtctaa aaatttagaa attaataata tcataacata cttttaaagc
     4501 aaaaaagggt aaattactta agctttattt gaaaaacgaa aaatgtctaa atacactgta
     4561 gtaactactt ttcaaaatga ataaagtggt tacagttaat gtacttagac agtataaaat
     4621 tatgaatctt taaactgtga tggtctttgt aaattaatta ttggatttgt ccatttacaa
     4681 acaaagtttg tagataatac atatacgatg attacagata tacttattaa ataaagataa
     4741 gtcattaatg atataggatt atcgaatggg taccattcaa atcctctaac aatgccaata
     4801 attaaaccat gtaataaata tacgtatagc gtacgactac caatataagt atataatttt
     4861 ttctttgttg acattaaatt tagaaacgca gtcattgcga ttaatataat tccatataat
     4921 ataagtcgtt taaaaggact gaatatactc tgtccttcat tttcaagtga agtatatggt
     4981 gaacttccca ataaccaatc tgcattgata ggatgaatca cgtaaacgat aaaaaacaaa
     5041 ataaaggtaa tgatagatac tggtattagt tttttatttt taaaaatagc cgtatgtttt
     5101 ttggtgaaaa tgtaacctag ataaaatatt gggaaaaata cgattgtcct tgaaatgctt
     5161 aagtagctat cgatgttatc tgaaaaacct gctccaatag atataataat tgaaactgat
     5221 agcactttat atggattaaa tcttctaact attactaaaa tgacatgaaa gaaaaatagc
     5281 gtgatcaaaa accataacgc aaatactggg ttaaaaggat caagttgtaa ttcgtcactt
     5341 ttacctgtta agaaataata aattgaaaag aatgcaaaaa atatcatata aggtactatc
     5401 aaacgttttg aaattttttc taaatagtat ggtttatcaa tatttttcgc gaaataacca
     5461 gatataaaca aaaatgttgg catatggaaa ctataaataa ctaggtataa tgctgataaa
     5521 tatttatcgc cactagtgta gggttgtaac atatgtccaa atacgactaa taatattaaa
     5581 attgctcttg cgttgtcaaa aaaataatct ctctctttta atgaagtcat attttatctc
     5641 ctttatgtcc ataagactct tactaattta ttatattttg tattagttat gcaacaaatt
     5701 tgtctattgt gtcgaatagt attgtaaaaa ttataaaaaa agcatataat attataaata
     5761 atatgtagtg gagtagaaca acatgtataa acaacttgaa aaacttatta cactgactaa
     5821 caatgactta aacttagtga atagaagatt tggacaacgc acggatatca aatctgaaca
     5881 gctagaactt ctccgtattt tatttaatta cgatcgctta tcacagtatg atttgacgat
     5941 gaagattagc agggaacaat ctatagtttc aaggtggatt aagaaattag ttttgaaagg
     6001 gtacatcaca agtcaacaat ctagcgaaga tttaagatgt aaagaattaa ttttgactga
     6061 tcaagcacgt acattaattt cacaaataaa taatgcacgt tgcgaattga ttgaagcaag
     6121 atgtcaatgt ttatcggaag tcgaattaga caatttaaat caattacttg ataagttaaa
     6181 tcaacgacgc atatcgttgt aacacgacaa tagcaaacac ataatttagt gtcgttgtgc
     6241 acacaacaaa aattgaatca tgaattacaa gcaaaagtag cggtgattgt taaaattgat
     6301 ggtaaacaat caccgctatt tttgcttgtg tatgtataaa aaagggatca aaggtcatcc
     6361 cccatgattg atagtggggg atgacttttg atcctatgtt catgttgctt atttatattg
     6421 tgggatgtcg aagtatttgc cgacttcgcc aattttatca tagtagcctt tgatgatttt
     6481 agcattgatg ttagcccaat ctacatctgt agcatattgg tgtgttcctg gatgtgcagg
     6541 attccatctc attttgtaaa gtgtattttg accagctttt acatatgagt tgccgatgaa
     6601 tttagcacca ccaacgattg cttttgatac tgtgtcccaa ccagcttgtt tagcatattt
     6661 aataccttca cgtaaaggat cgttatcata tgcagcaata ccaaatacgt tatggtattt
     6721 cgtgtttgag ttagttacaa ctttgttgtt cactacatct gcacctttcg ctaattgaga
     6781 agtaccgtta cctgtttcta atagggcatg tgagataaga taaacttcat taatgccata
     6841 catttgagca gctttgttaa atgcagcacc ttggttttct aatacacctt taccttttaa
     6901 gaattgatta attttatcaa tagaaatatt ttgtggttgg tctaagcgta agaattgata
     6961 ttttaatgct ggatcttgag ctaaacgctt cgtatccatt gcatgcttaa catcattaaa
     7021 gttagcatct gtccacttac ctggtacacg ttgtacttgt ggtttatatt gtaaaccagc
     7081 ttgtatttga gcaacttggt ttaatgtcat acctgtttga ttatacttaa ttaattcttt
     7141 agctaaatca gttgatttaa tccatgctaa tttaccgtta gataatttac catagtacca
     7201 agtttgtcca ttaatgactt gttctttaac aactgcgaat ggttgttcat taaatgcttt
     7261 taatgagtat ttagctgtat cagaatttgg tgttacatag taataaccat taccattttt
     7321 aattacataa gtgtagttat aatctttggc agctgatgta gttggtttca cagcggttgg
     7381 tgcagttaaa tcttttgcat ttacccaacc agtgcggtta ttaatagtac cgtataaata
     7441 aacatctttg cctacagata cttgtttcgt tgcattaaat gtaccttgag caatgttatt
     7501 gcctgttaaa atgacttggt ttttagtacc ccaaggaacc attgataagc cgttatttga
     7561 tttattaaca gtatattttt gagtcgtttt aacttctttg cctaagtttt gaacatttaa
     7621 gtcttttaca ttgaaccaac ctaatgggat gttatggctt gtattgttta ataatacata
     7681 cgtttcatta ccatgagcac gctcttttgt tacatagaac gtacggtctg catatttcgc
     7741 accgtttttc gctgtttttt cataaacaga agcacgaata ccagtgttgt ttggtttaac
     7801 ttgagcaatc ttgctaactg tttgagtcgt ttgtggttta gtaacagtat aagcttttac
     7861 agctgttttt ggttgtgcta ctgctttttt aggtgcagca ggtacagcta aatatgcttt
     7921 acttacccaa ccagatttac catttacagt tccaaataaa tagatagatt tatcaatttg
     7981 ttgttgctta gtcgctttaa aagtttggtt acctgtacca gaaactgcac cagcttcttg
     8041 tttataagtg ccccaaggta ctgaatataa tttagtgcct ggttttactg tatatgtttg
     8101 cattacattt acaggtgatt ttgcattgtt ataaataacg tcaccttgtt taacccaacc
     8161 aattaaagtt ggactattgt aatctttaac taagtagaat ttgtttccac ctaaacttgc
     8221 ttcttttgtt acagcaaatg ttttttgaac ttctttcgtt ggcttaccag ttttgtcata
     8281 aactgtagtg aataagccat tgtttttagc attaatttga gcaacaccgt ttaatgatga
     8341 aactgttaat ttattatttg ttgtaggtgt tgatggctta ggtgttggtg taggcgtagg
     8401 tttagcagta tcaactaaat atgctttact tacccaacca gatttaccat tcacagagcc
     8461 atataaataa attgatttat caatttgttg ttgctttgaa gccttaaatg tttggtttcc
     8521 agagccagac acactaccag caacttgttt agatgtaccc caaggtactg tataaagttt
     8581 cgtaccaggt ttgattgaat atgattgatt tacatttaca ggtgatttag ctgtgttgta
     8641 aaccacatcg ccttctttaa cccaaccaaa tttattacca gaattgtaat cttgaacaag
     8701 atagaatttt tgattaccta atgtagctgt tttagataca gcaaatgttt tttgaacttc
     8761 attagttgct ttaccagttt tgtcgtatac agtagtatat aaaccactat ttgttggttt
     8821 gatttgtgcg acaccattgt ttgcagcaac tgttaattta ccagttgatg gtttcgacgg
     8881 tgttgttggt tttgatggtg tagtaggggt agttgtagat tgcgtacccc atggcgccac
     8941 tttacccatt tttattaaat atttttcatt aattaagtca tataattgat cataactata
     9001 attatgactt cttaaatatc catgtggatc ggcatggtca gtaccaccta aatatttact
     9061 tacagcgtag tgagtccata ctgtaccatt tccatcatac tcagcactgt ctggttttaa
     9121 accataatat tgtaattgtg tagctgcata gtcagcatag ttattcattg aacgtgcaaa
     9181 tgaagcatag tcgtgtgtgt gtacgatttc aacattgatg aatctagggt taccgactgc
     9241 accgacaccc caagataagt aatccgttgg tgctgtttcg attatacgat ccccatcaac
     9301 aaatgcatgt acgaatgcgt tttgatagtt atttttcata taactaattt caccatttat
     9361 cgtcgaacga tcattagctg tatcatgaac tacgatacct tcaggacgac ctacgccgtt
     9421 acggtatgcg tatttaggga agtaagatgt ataatcttct tcaattttag gtgcttttaa
     9481 gttattttta cgaatgtaat cgttaattga agagtttact tgtggtttat attttggcaa
     9541 actcgttttt ggtgttgcag caactgatct tggttgtgct gaagcgctaa aagtagttac
     9601 tttaggtgtc gcttcagttt tagctttagg tgctgatgta gttgcagctt taggtgctgc
     9661 ggttttatat tgcgtttcaa gagctgcagg tttagcagct gatttaatta attctggatt
     9721 aatttgattt tctgaattat catcttcatc atcaactaaa ctataaccag catttgtaac
     9781 attagtgtta gttttaggtg ctgtagtgct tgttgacttt gcaacaggct gcgtattatt
     9841 tgtagtcgct gattgattag cacgagtgtc accatttact tgtgcagtat caactttttg
     9901 acttacttga gcattgcctg ttttgttatt tgctgttttt ggttggacaa tagcagggtc
     9961 ttgatatact tgagtgccag aaatgttttg cgttggattt tttacctcag cttttgcttg
    10021 ttcagtagtt gctttaactt tattactatc taaaacgttt ttattagtag tttgatcttg
    10081 tgtcgtctca gctgcttgaa cttgatgtgc agtgactgct gaacctacaa gcgttaatgc
    10141 aaccattgat ggtagtttgt aattgaattt tttcgccatt ctatttatta ctcctaacat
    10201 ttattaatta ttactaacat tatagtacct gttttatata cctgtgcgta tttaaccaat
    10261 ttgtaatata ctcttaattt acattttcac tgtaaaaaaa tgaaaaaaat tttgaatctc
    10321 aatggtgacg tgacaaatca aatagaattg atgtcaattt aagaattggt ttattacaaa
    10381 tgtttctaaa atagatacaa ctttttttaa ttaagcgagg ttaacttttt tgtcatttca
    10441 atatgctcga tgccttcctc aagaaatata ttacctctca ttttaaagtt taaactttca
    10501 taaaatggga tagcatgaca ttgggcattc atagtagcta cgtaaaaacc ttcatcttta
    10561 gctaatgatt ctacagcttg cataagcatt ctacccattc cttgtccacg atgtgatttc
    10621 atcacagcta ctcgttctat tttgacagtt gtttcattaa tagggcgtat tcgagcagtg
    10681 gcaactggct gtccattatc atatccaatg aggtgaatag attcagattc atattcatca
    10741 atttcacttt cctcagggac gccttgttct tctacaaaca ctttctttct tatatagaag
    10801 caatcttcta acatcttttg attgtttact tttgaaaaca taaaattcct ccacatccta
    10861 acaaaatcat ttacatttaa tttactaata ataacataga ataacgattg ttattttcct
    10921 caaaaataat tagacataaa aaagctataa agatgattgt tgtgaatcat ctttatagct
    10981 aattaattat taatgttata gtgcttcttt tctaataatt gctgtatatt gccaaaagat
    11041 tcgcatttga gcaatattcc aattattcat tcccattgta aatcctgaag gcttaaatat
    11101 attcacatca gttacttcta aagctgctgc aactaaatat tgtaatggaa tgctaagtga
    11161 tttcattgtt tcggtaatgc cattttcatc atatacccat gaaaatggta actctgattc
    11221 gaaaacatca acactattga aaatttcagg tagggctaaa atataacatg caccatctac
    11281 aataggattg tcgatactat ttttgtaata aattaggagt gcttcatagt tttctcgatg
    11341 ctcatgattg acgtaaaaaa attcacttct aaaatgagcc atatcttggc tatgaatgat
    11401 ttgttgttct aacatattaa acatgccatt tatatttgca atcttttcgt atgtttttct
    11461 actcatgtat gatgctcctt tcataaatag taaatgttaa tcgtcatata atattaattt
    11521 acaacaccat tttggttatt tgaagcttgt ggcgcttgtt gtgtgccacc ttgattttga
    11581 tttgagtttt gatctgtagc aggttgttgt tgattgctgg aatcactgtt attagttgaa
    11641 tcactgttct cgttagatgt cttatcttta tctgtcgtat cattttgttc ttttctcaat
    11701 aaactattat ctaaaggcgt taatggtatt aatgaaccat aatgattaat gacacgttga
    11761 tctaagaaat catttttatc attaataggt gataattcta agtctttacg aagtaagttt
    11821 gcatattttt gaatgctttc aacacttgga tgataatagt aaataccatt taacatatca
    11881 tctttacctt ttaattgcgc agttttaatt tcaacatcat ttgttaagta catttttgct
    11941 aaagctttaa tttcagagtt agttaaatta tgctttgcat ttttacctac aatttgaatc
    12001 acgttatcaa gtttatcaat agaatcaact tcctgtgctt tttggaataa aatcttaatt
    12061 aattccattt gacgttgtcc acgttttaag tctgaatcat gatgtctagt tctagcaact
    12121 gctaaagcct catcaccatt taatttttgg tacccttttt taattttaat cttaccagta
    12181 tcatctgtgt taggttcatt taagtcgtat ggcacatcat agtatatgcc accaagctca
    12241 tttacagcct cgacaaatgc tttcatattg actctcacat aataatcaac aggtacattc
    12301 atggtagctt ctaccgaatc cattgcggca attggaccac catatgcatg tgcatgggta
    12361 atcttatcgt aatagccaac tttaggaatg tagctgatag tatcacgtgg aatactaagc
    12421 attctaattt gatgttttga ttgattaaaa gtagttaaaa tcatagcgtc tgatctagag
    12481 tgttcagcat cctgtccttt ttttcttctt ccatcgttat catcgatacc taagaaaaga
    12541 atagagatag gttgttcttc gggattgact ttattatctc ttaagttgga ttgacgatta
    12601 gcatttttgc tgtcttgaga agattcgaat gcatcttggg acgttttaaa aagtaacgta
    12661 gcgaagacta ttggaacaac aatgagaacc aatgctagaa ggatcaaaaa gtattttaaa
    12721 aatttattca tgattgatgc tcctatatta aatttttgtt aaatcataat cataatttta
    12781 aaatataatt agttgaacga tatttataca acttcattac taatacatta tagccttaaa
    12841 ttgtagtact ttatagtaaa aaatacaaat gttatgtcaa agttccttgt aatttgaatg
    12901 ttttgttaaa atcaaaaagc atttttaaaa atataatgcg actttagtat gatgtcttaa
    12961 aaaatttaaa ggcgtaatat aaattcgagt gatttataca aagtgataag gtataattaa
    13021 ttttgttgta tagataacat attagaaaaa cttattacta tgtagcgtaa agcataaatg
    13081 aatgataagt taatttttgt aagt
//
LOCUS       DALAGF010000078        13104 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.078, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000078 DALAGF010000000
VERSION     DALAGF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13104
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.078"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(120..1022)
                     /gene="cvfB"
                     /locus_tag="O2692_002001"
     CDS             complement(120..1022)
                     /gene="cvfB"
                     /locus_tag="O2692_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HCZ4299623.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDYLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKIALTKKGWSRMDSKE"
     gene            1169..2770
                     /locus_tag="O2692_002002"
     CDS             1169..2770
                     /locus_tag="O2692_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ4299624.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNISFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSYKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            3630..4835
                     /locus_tag="O2692_002003"
     CDS             3630..4835
                     /locus_tag="O2692_002003"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCZ4299625.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGMNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIISNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            4899..5888
                     /locus_tag="O2692_002004"
     CDS             4899..5888
                     /locus_tag="O2692_002004"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCZ4299626.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            5890..6777
                     /locus_tag="O2692_002005"
     CDS             5890..6777
                     /locus_tag="O2692_002005"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCZ4299627.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNIEALKTHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLRETYDTFKAGENE"
     gene            6774..7496
                     /locus_tag="O2692_002006"
     CDS             6774..7496
                     /locus_tag="O2692_002006"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCZ4299628.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKGADVAIDFSNPNLLFPLLDEDFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            7523..8242
                     /gene="dapD"
                     /locus_tag="O2692_002007"
     CDS             7523..8242
                     /gene="dapD"
                     /locus_tag="O2692_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCZ4299629.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            8385..9536
                     /locus_tag="O2692_002008"
     CDS             8385..9536
                     /locus_tag="O2692_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCZ4299630.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            9541..10626
                     /locus_tag="O2692_002009"
     CDS             9541..10626
                     /locus_tag="O2692_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCZ4299631.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTLKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            10616..11881
                     /gene="lysA"
                     /locus_tag="O2692_002010"
     CDS             10616..11881
                     /gene="lysA"
                     /locus_tag="O2692_002010"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCZ4299632.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(12121..12522)
                     /gene="msa"
                     /locus_tag="O2692_002011"
     CDS             complement(12121..12522)
                     /gene="msa"
                     /locus_tag="O2692_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarA expression modulator protein Msa"
                     /protein_id="HCZ4299633.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINILAAMMIVLVIPIMISGI
                     LFFKTNIDKTYIFFNIIFIDFYYYIYNVHLMTLPKFNNYIKAEMMELEDIDVLITSKD
                     FGFDEILFYTLYLLLILIVLYYLKKQVKHKI"
     gene            complement(12719..12919)
                     /gene="cspA"
                     /locus_tag="O2692_002012"
     CDS             complement(12719..12919)
                     /gene="cspA"
                     /locus_tag="O2692_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HCZ4299634.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
BASE COUNT         4591 a         2065 c         2336 g         4112 t
ORIGIN      
        1 cctattcgtt tgaattattt aattttttac tttgaaagga atgtttaaaa aactacttca
       61 tctgtccgat ttaatattga ataaaaaaaa cacgcatcct acgacacgtg taaaaatgat
      121 tattcttttg agtccattcg actccaacct tttttagtta aagcgatttt acctgtttct
      181 atattaataa tcttctgttt atataagtga ccgattgcac gtttgaatga acctttactc
      241 atattgaata cttctttaat cgcttcaggg cttgatttgt cccagaatgg taattcacca
      301 tcatattcaa ctagtaaatc aaagatgact tggccgtcat cgtctaaacg ttcatgtgca
      361 agtggtaaaa atgaaccatt taactcacct ttatcattat gcccgataat tctaacttga
      421 acagattcac ctaatcttgg ttcagcttta cgttctgatt catgtacgaa aattttgtaa
      481 cctgattcgc ttaataaaaa gctaccaatt cgtaatacgc ggtaaggttt ggcttcaatg
      541 acttcgtttt ttaaattatc gtcgtgtaca ggtgtaaaca tattttctac aacagattca
      601 ctcgctaaac gtccatacat atgattctca cggtcaattc gtaatgtaac tagcaaataa
      661 tcaccaggtt gtggccatag tgatttcact tttggtaaat cttcccatgg tactaacact
      721 tcacggggta atccaacatc tatacgtgcc ccatcgcgat ccgttttaag tactttagca
      781 aagtcatatt tatctttcgt aatatcaggc atattttgag ttgcaaataa ttcacctgaa
      841 cggtttggat aaatgaagaa actatattct tcacctactt ctaattcatc atcatcgttc
      901 atttctgatt ggtttaactt tacgttttca ccgtttggtc cttttaaaag gtaagttgaa
      961 ccttgtaacc ctactacttc aaggaattct atagaaccta ctatatcttt gtctaatgcc
     1021 atgttattct ccttcgattc ttgtattctt tattattata acatgtgtga gtcattctat
     1081 cattacaact tatatgaggt cgaaactgtg acgataatat gatataattc ttccgttaga
     1141 atatcgaatg aaaaaaagga gaaaatgcat gttacaagta actgatgtga gtttacgttt
     1201 tggagatcgt aaactatttg aagatgtaaa tattaaattt acagaaggta attgttatgg
     1261 attaattggt gcgaatggtg caggtaaatc aacattctta aaaatattat ctggtgaatt
     1321 agattctcaa acaggacatg tttcattagg gaaaaatgaa cgtctagctg ttttaaaaca
     1381 ggaccactat gcttatgaag atgaacgcgt gcttgatgtt gtaattaaag gtcacgaacg
     1441 tctttatgag gttatgaaag aaaaagatga aatctatatg aagccagatt tcagtgatga
     1501 agatggtatc cgtgctgctg aacttgaagg tgaatttgca gaaatgaatg gttggaatgc
     1561 tgaagctgat gctgctaacc ttttatctgg tttaggtatc gatccaactt tacacgataa
     1621 aaaaatggct gaattagaaa acaaccaaaa aattaaagta ttattagcgc aaagtttatt
     1681 cggtgaacca gacgtactat tactggatga gcctactaac ggtctcgata ttccagcaat
     1741 cagttggtta gaagatttct taattaactt tgataatact gttatcgtag tatcgcatga
     1801 ccgtcatttc ttaaataatg tatgtactca tatcgctgat ttagacttcg gtaaaattaa
     1861 agtttatgtt ggtaactatg atttttggta tcaatctagt cagttagctc aaaagatggc
     1921 tcaagaacaa aacaagaaaa aagaagaaaa aatgaaagag ttacaggact ttattgctcg
     1981 tttctcagct aacgcttcta aatctaaaca agcaacaagt cgtaaaaaac aactcgagaa
     2041 aattgaatta gatgatattc aaccatcatc aagaagatat cctttcgtta aattcacacc
     2101 tgagcgcgaa atcggtaatg acttactaat cgttcaaaat ctatctaaaa cgattgacgg
     2161 tgaaaaagta ttagataata tttcattcac aatgaatcca aatgataaag caattttaat
     2221 tggggatagt gaaattgcga aaaccacatt gcttaaaata ttagccggtg aaatggaacc
     2281 agacgaaggt tcatataaat ggggtgtaac aacgtcatta agttacttcc ctaaagataa
     2341 ctcagaattc tttgagggcg ttaatatgaa ccttgtagat tggttaagac aatacgcacc
     2401 agaagatgag caaactgaaa catttttacg cggcttctta ggccgtatgc tatttagtgg
     2461 agaagaagtt aagaaaaaag ctagtgtact ttcaggtgga gaaaaagtac gttgtatgct
     2521 aagtaaaatg atgttatcaa gtgcaaacgt tcttttactt gatgaaccaa caaaccactt
     2581 agacttagaa agtattactg ctgttaatga tggacttaaa tcattcaaag gttctattat
     2641 ctttacttca tatgactttg aatttatcaa tacaatcgca aaccgtgtta tcgatttaaa
     2701 taaacaaggt ggcgtttcaa aagaaattcc atatgaagaa tatttacaag aaatcggcgt
     2761 tttaaaataa acataatatg tagaatttct tttcgaaaat ctctgtattg aggcccgacc
     2821 aacttgcaca ttattgtaag ttggcttact gtcaccttct atattgggac ttattccgca
     2881 acttgtattg tttgtagaaa ttaggaatcc aatttctcta tgtgttgcac cgacaaggat
     2941 tgaaaaaagc tagctacaaa gtcattttca ttcagtcaac tactgtcaat ataacattgc
     3001 agggcctagg gcattgattc atgtccgaga ctatattcaa tttaatagac attaactggg
     3061 cgtctcgatg ctaatattta tagcatcgag gtgtcctttt tattttattt tttaacacat
     3121 cagcatagtg aacacccctt ctctaaatta caaatacagt tatcaacgca ttaaaagagt
     3181 acatttattt aatgtgcata ttgattatgc tataagtatt ttgaatttta tttttcaaaa
     3241 agtctcatta tcaccacctt ttaaaatata agcaacgcta taacttgaat tttcaggtta
     3301 ttccaaaaaa tctaaattat cggtttacat tcatatttgt catatgtata atgaacacat
     3361 accaatttaa taatattttg atgaggcgca tcaatcatga gtaaagttta gattactgtc
     3421 tgctaacagc taaatttgaa agggtgcgat gccgaagcaa ttataatagc agttataatt
     3481 tgttggactt tttggttaag agctgagagt ttgtcattat ttaaaaataa tggagtgcat
     3541 cacttgtata tagattaaga gcaagttcgc attccgaact tgttcttttt ttattattgt
     3601 gtgcccttcc taacaattag gaggattata tggtaacaag aagtgtgttg aaatttggcg
     3661 gatcatccgt cagtgatttt acaaaaataa aaaggatcgc tgaaatgtta aaggagcgag
     3721 tcaatcaaga tgaacaatta attgtcgttg taagtgctat gggtaacaca acagatcaat
     3781 taatgacgaa tgtatcaacc ttgactaaag caccaaaaca acaagaactg gcattattat
     3841 tgacaaccgg agagcaacaa actgtatctt atttatcaat ggtattaaat gatatcggta
     3901 tgaatgccaa agcaatgact ggctatcaag cgggtattaa aaccattggc catcatttaa
     3961 aaagtaaaat tgctcaaatt aatcctcaaa catttgaaca agcctttcaa gaaaacgata
     4021 ttttagtagt tgctggattt caaggcatca atgaacatca ggaattaaca actttaggca
     4081 gaggtggttc tgatacgacc gctgtggcac ttgctgttag taatcaaata ccttgtgaaa
     4141 tttataccga cgttgatggt gtgtatgcca ctgacccaag acttttacca aaggctaaac
     4201 gactagacat cgtctcatat gaagaaatga tggaaatgag cgctttaggt gctggtgtac
     4261 ttgaaacaag aagtgttgaa ttagctaaaa actataatat ccctttatat ttaggaaaaa
     4321 ctttatcgaa cgtgaaagga acatggatta tatcaaatga agaaatatta gagaaaaaag
     4381 cagttactgg tgtggctttg gataaacata tgatgcatgt aacaattagt tatcccctac
     4441 ctgacaatca gctacttacc caactattta cggaacttga agaaggtgct gtaaatgttg
     4501 atatgatttc acaaatcgtc aacttggatg ggctacaact atccttcacg attaaagata
     4561 gtgattttca tcaaatttct atgattcttg aaacattaaa gaatcaatat gaagcattag
     4621 cttataaaat caatgagcat tatgtcaaaa tttcattaat tggctcaggc atgcgtgata
     4681 tgtcaggtgt ggcatcaaaa gcatttttga cattaattga aaataatata cctttctacc
     4741 aaacaacaac atctgaaata agtatttcat acgtcattga tgattttaat gggcaacaag
     4801 cggtagaaaa actatatgac gcatttaaca tttaatggta aaatgattgt taaaatattc
     4861 taaaaattgg aaattattat aaaatggagt gacaagttat gacaaagtta gcagttgtgg
     4921 gtgcaacagg attagtaggt acaaaaatgt tggagacatt aaatcgtaaa aatattcctt
     4981 tcgatgaatt agtattattt tcatcagcac gttctgcagg gcaagaagtt gaatttcaag
     5041 gaaaaacata tacagttcaa gaattaactg atgctcgtgc aagtgaacat ttcgattatg
     5101 tattaatgag tgctggtggc ggtacaagcg aacactttgc cccacttttt gaaaaagctg
     5161 gtgcaatcgt tatagacaat tcaagtcaat ggcgtatggc agaagatatt gatttaatcg
     5221 ttccggaagt caatgaacct acatttacaa gaggtatcat tgccaatcca aactgctcta
     5281 cgattcaatc tgttgtacct ctaaaagtat tgcaagatgc ttatggttta aaacgagtgg
     5341 catatacaac atatcaagct gtatcaggtt cagggatgaa aggtaagaaa gatttagctg
     5401 aaggtgtaaa tggtaaagca ccagaagcat atccacatcc aatttataat aatgtgttac
     5461 cgcatattga tgtgttttta gaaaacggat atacaaaaga agaacaaaaa atgattgatg
     5521 agacgagaaa aattttaaat gcgccagact taaaagtaac agcaacatgc gcacgtgtgc
     5581 ctgttcaaga tagtcatagt gttgaaattg atgtaacgct tgacaaagaa acaacagcag
     5641 aagatattaa agcgttattt gatcaagatg accgcgttgt tttagtagac aatccagaga
     5701 acaatgaata tccaatggca atcaattcta ctaataaaga tgaagtgttt gttggccgta
     5761 tacgtagaga tgattcatta gaaaatactt tccatgtatg gtgtacatca gacaatttat
     5821 taaaaggtgc tgcattaaat gctgtacaag tattggaaca agttatgcgt ttaaaaggag
     5881 cgaattaaaa tgacacattt atttgagggt gttggcgttg cacttacaac cccttttaca
     5941 aataacaaag ttaatattga agctttgaaa acacacgtta attttttact agaaaataat
     6001 gcccaagcaa tcatcgttaa tggaactact gctgagagcc ctactttaac aacagatgaa
     6061 aaagaacgca ttctaaaaac agttattgat cttgtagata aacgtgttcc tgtcatagca
     6121 ggaactggca ctaatgatac tgaaaagtca atccaagctt caatccaagc taaagcctta
     6181 ggggctgatg caattatgtt aattacgccc tactacaaca aaacgaacca acgtggttta
     6241 gtcaaacact ttgaagcgat tgcagatgct gtgaaattac cagtcgtgct gtacaatgtt
     6301 ccttcaagaa cgaacatgac aattgaacca gaaactgtag aaatattaag tcaacatcct
     6361 tatatagttg ctttaaaaga tgctacgaat gattttgagt atttagaaga agtgaaaaag
     6421 cgcattgata caaattcatt tgcattatat agtggcaatg atgacaacgt cgtcgaatac
     6481 tatcaacgtg gcggtcaagg ggttatctct gttattgcca atgtcattcc taaagaattt
     6541 caagcgttat acgatgctca acaaagtgga ttagatattc aagatcaatt taaaccaatc
     6601 ggcacactgt tatcagcttt atcagttgat attaacccaa ttcctattaa agctctaaca
     6661 agttatttag gatttggaaa ttatgaatta cgtctaccat tggttagcct agaagataca
     6721 gatactaaag tgcttcgtga aacatatgac acatttaaag cgggtgaaaa tgagtgaaaa
     6781 tattactaat tggctatggc gcaatgaatc aacgcgttgc tagattagca gaagaaaaag
     6841 gacatgaaat cgttggggtc attgaaaata caccgaaagc aacaacgcca tatcaacaat
     6901 atcaacatat tgcagatgtt aaaggtgccg atgttgcaat agatttttca aatccaaatc
     6961 tgcttttccc tttattagat gaagattttc atttgccatt agttgtggca acaactggcg
     7021 agaaagaaaa actacttaat aagttagatg aattgagtca aaatatgcct gtgtttttca
     7081 gcgcgaacat gagttatggc gttcatgcat tgactaaaat tttagcagct gctgttcccc
     7141 tacttgatga tttcgacatc gaattgactg aggcacatca taataaaaaa gtagatgcac
     7201 caagtggtac gttagaaaaa ttgtatgatg tgatcgtatc tttgaaagaa aatgtaacac
     7261 ctgtgtatga tagacatgaa ttaaatgaaa aacgccagcc acaagatatt ggtatacatt
     7321 ctattcgtgg aggtacgatt gtcggtgaac atgaagttct atttgctggc actgatgaaa
     7381 cgattcaaat cacgcatcgt gcacaatcaa aagatatttt tgcgaatggt gcaatacaag
     7441 cagcagaacg cttagttaat aaaccaaacg gcttttatac gtttgacaac ttataaacat
     7501 attaaaggag atcgattatt ttatggtaca acatttaaca gctgaagaaa ttattcaata
     7561 tataagtgat gctaaaaagt ctacaccaat aaaagtatat ttaaatggta attttgaagg
     7621 catcacatat ccagaaagtt ttaaagtatt tggttcagaa caatctaaag taatcttttg
     7681 tgaagcggat gattggaaac ctttttacga agcatatggt agtcaattcg aagatataga
     7741 aattgaaatg gatcgtcgca attctgctat tccattaaaa gacttaacaa atacgaatgc
     7801 acgaattgaa ccaggtgcgt ttattagaga acaagccatt attgaagatg gtgctgtcgt
     7861 tatgatgggc gcaacaatta atattggcgc agtcgttggc gaaggtacaa tgattgatat
     7921 gaatgctact ctcggtggtc gtgctacaac tggtaaaaat gtacatgtag gggctggcgc
     7981 agtattagca ggtgtgattg aaccccctag tgcttcaccg gttataatcg aggatgatgt
     8041 attaatcggt gcaaatgcag ttattttaga aggtgtacgt gttggtaaag gtgctattgt
     8101 tgcagctggc gcgattgtga cacaagatgt accagctggt gcagttgttg ctggtacacc
     8161 tgcaaaagtg attaagcaag cttctgaagt acaagatact aaaaaagaga ttgtagcagc
     8221 attaagaaaa ctgaatgact agtgacgtca acgtataata atttcaaggt tgagatacga
     8281 ttatgtctca accttattgt ttagcattga ataaacttat gatcacaaat ttcgaaatac
     8341 gttacactat ttatatagta atttaacaca aaggattgat aattatgaat gaattagaat
     8401 ttgttacgaa acatcgccgt catttacatc aacatcctga attaagctta catgaatttg
     8461 aaacaactgc ttatattaaa gcgtttttag atagtttaaa tattaaatac gattgcccat
     8521 tggaaactgg cgtcattgca tacttagaag gtaatggctc acatacgata gcgtatagag
     8581 ctgatattga tgcgttacct attttagagg aaaatgatgt gccttatcgc agtcaatctg
     8641 atcatgtgat gcatgcttgt ggacatgatg gtcatacaac tgcattaatg ctttttgtac
     8701 aacgttgcaa agacatgcaa gatgcaggtc aattaccgca aaatgtcgtt ttcattttcc
     8761 aacctgcaga agaaactggt ggcggtgcaa atcgattaat aaaagccggt gcctttgata
     8821 agtatccaat tgaagcggta tttggtattc atgttaaccc atttgctgat gaaggcattg
     8881 cagtgataag agatgaagaa attacggcca gcgcaacaga gtatcgcttt ttcttaacag
     8941 gcctgtcaag tcatgttgct gataaagaac aaggtcattc ttgtggtgaa gcattacaac
     9001 atgtattaac tcaaatatca caaattcaac aatttcacct taacggtttg aaacgaaata
     9061 ttgttcatat tggtcatttt aaagctggtg aagcgattaa cactgtacca agtaatggct
     9121 atttagaagg tactattcgt acatatgata ttgatgattt aacaatcgtt aaaaatcaaa
     9181 tgcacaagat agcagaaagt gtcaagcttc tgtttaatgt agattgtgaa gttaaatttg
     9241 cagaaggtta tccccctaca atcaatagtc cgaaattacg tactcaaata gaggacgcct
     9301 taataaaagc tgatttaaat gtctatgaca aaccaacgcc attcttattt ggggaagatt
     9361 ttagttttta tggtcaacaa ctagctccag cttactttgt ttttatagga acacgaaatg
     9421 aagataaagg ttttgtaact ggtttgcaca catcacattt aaattttgat gaaaaagtgt
     9481 taataaacgt ggttaatttt tacgaaaatt tattaaataa ttacaaagag gtgtaataca
     9541 ttgacagcaa catggtctgt aaataagaaa atatttttac aaaatgcaat cacagtcaaa
     9601 aacaatcagc cattaatggc agttgttaaa aataatgcat atcactatga cctagaattt
     9661 gctgtaactc agtttatcca tgcaggtata gatacattta gcacaacatc actacgagaa
     9721 gcaattcaaa ttagacaact tgctccagat gcaacaatct ttttaatgaa tgcagtttac
     9781 gagtttgatt tagtccgtga acatcaaata catatgactt tgccgtcgtt gacatattac
     9841 tataaccata aaaatgattt agctggtatt catgttcact tagaatttga aaatttatta
     9901 catcggtctg gatttaaaga tttaaacgaa attaaagaag tattgaaaga tcaccatcat
     9961 aatcaaaatg caaaaatgat tattagtggt ttatggaccc attttggata tgctgatgaa
    10021 ttcgatgtgt cagattataa tgttgaacgt tcacaatgga tggaaattgt tgaagcactt
    10081 ttatctgaag gttatcagtt cgacctaatc catgctcaaa atagtgcgag tttttatcgg
    10141 gaaggacaaa tattactacc ccaccataca catgcgcgtg taggtattgc gttatacggt
    10201 tcaagaccat atagttcact gaatcaacat gatatagttc agtcattaac tttaaaagca
    10261 catgttattc aagtgcgcga agtacaagct ggtgattatt gcggttatag ctttgccttt
    10321 gaagtgacta aaaacaatac aaaattagct gtagttgata tcggttatgg cgatggaatt
    10381 ttaagaactc gtgctaaaca tgaagcactt atcaatggta aacgctaccc gatacgtgca
    10441 ttaatgatga gccatatgtt tgttgaagta gatggcaatg tacatgcaca agatgaagtt
    10501 attctttata ataatgatat ccgcatcgat gaatatacct ttaaaggtgt tggtgcaaat
    10561 tctgaacaat taagtgctat gaatcatgat tctttaaaaa aggagtacat ttcaaatgac
    10621 tgttaaatat aatcaaaatg gcgaattaac aatggatggt attagtttaa aaacgattgc
    10681 acaaagcttt ggtacaccta ccattgttta tgatgaacta caaattagag aacagatgcg
    10741 ccgttaccat cgcgcattta aagatagtgg attaaaatac aatatttcat acgcctcaaa
    10801 ggcatttact tgcattcaaa tggtcaaact tgtagctgag gaagatttac agttagatgt
    10861 tgtttctgaa ggtgaattat atacagcttt agaagcaggt tttgaaccga gtcgcatcca
    10921 tttccatggt aacaataaaa cgaaacatga aattaggtat gctttagaaa ataatatcgg
    10981 ttattttgtt atagattcat tagaagaaat tgaattaata gaccgctatg ctaatgatac
    11041 ggttcaagtt gtattacgag ttaatccagg tgttgaagca catacacacg aatttattca
    11101 aactgggcaa gaagatagta agtttggatt atcaattcaa tatggcttag ctaaaaaagc
    11161 aattgacaaa gtccaacaat ctaaacactt aaaattaaaa ggtgtacatt gtcatattgg
    11221 ttcacagatt gaaggtacag aagcatttat tgaaactgct aaaattgttt tacgttggct
    11281 taaagagcaa ggcattcaag ttgaattatt aaaccttggt ggtggctttg gtattaaata
    11341 tgttgaaggt gacgaaagtt tccctatcga aagtggtatt aaagatatta cagacgcaat
    11401 aaaatccgaa attaaagttc taggtataga tgcaccagaa ataggtattg aaccgggacg
    11461 atcaattgta ggtgaagctg gcgttacttt atatgaagtt ggaaccatta aagaaattcc
    11521 agagattaat aaatatgttt caatcgatgg cggtatgagt gatcatatca gaactgcact
    11581 ttatgacgca aagtatcaag cattgcttgt taatagaaat gaagaagcag atgacagtgt
    11641 aactatagct ggaaaattat gtgagtctgg tgatatcatt attaaagacg ctaaattacc
    11701 ttcatcagtc aaacgtggag actatcttgc tatattatca actggtgcat atcattactc
    11761 tatggcatcc aattacaatc aaatgcaaaa gccttctgtg tttttcttaa aagatggcaa
    11821 agcacgtgaa gttataaagc gacaatcgtt aagacaactc attattaatg atacaaaata
    11881 aaaataaaca aagtaatccc cgaagcacag aaaattatac tgtgactcgg ggaattttta
    11941 tgcattatag atgactgtag aaaacatgct tggacatata ctctaaaaaa ggaagaacag
    12001 cacattttta gttagaaagt atttatttct ttttgtcatt aatcctttat atttagttag
    12061 ccctattcaa ctaatttaaa gcatcatata cataccacac tttaaacaag catcatgaaa
    12121 ttagatttta tgctttactt gctttttcaa ataatataaa acgataagaa ttaacaacaa
    12181 atataatgta taaaacagaa tttcatcaaa tccaaaatct ttacttgtaa ttaatacatc
    12241 aatatcttct aattccatca tctctgcttt aatataatta ttaaactttg gcaaagtcat
    12301 taaatgtaca ttgtaaatat agtaataaaa gtcgataaat atgatgttaa aaaatatgta
    12361 tgttttatca atattagttt tgaaaaatag tatcccagaa atcattatcg gaatcactaa
    12421 tacaatcatc attgctgcta aaatattaat atttaatcca attgcagata atacaccgaa
    12481 aactaataaa ttagcaacta aactaaggat aagatatttc ataaaatcac ctcgtcgttg
    12541 tacaccataa ttatattata taaatatatt tttctattgc aaacaatgtt ggtgatataa
    12601 aaaaagagtt ctcaatttgg ttgagaaact catcgcgtta agtatttgta ataagcatat
    12661 aaaaacacct tacttcttgg taaggtgtta gttaaaatca atgattcaaa tctaagaatt
    12721 atagtttaac aacgtttgca gcttgtggac cgcggtcgcc ttcaactact tcaaactcaa
    12781 cagcttgacc ttcttctaat gatttgtaac catcttggtt aattgctgaa aaatgtacga
    12841 atacgtcatt ttctccttca acttcgataa agccgaatcc tttttcagcg ttaaaccatt
    12901 taactgtacc ttgtttcata atctgaaacc tccaagacta aaattcattc aatatgctta
    12961 ttcgcatatt acaaaaaata catgcaaatt tcactcaaat attctttgca atatggaata
    13021 atcatctgct taacttgtat tatagtgcag ttattaccaa attgcaatac aataataaaa
    13081 aatattcatt taatcaatta ttgg
//
LOCUS       DALAGF010000079        12956 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.079, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000079 DALAGF010000000
VERSION     DALAGF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12956
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.079"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            259..2013
                     /gene="eap"
                     /locus_tag="O2692_002013"
                     /gene_synonym="map"
     CDS             259..2013
                     /gene="eap"
                     /locus_tag="O2692_002013"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HCZ4299635.1"
                     /translation="MKFKSLITTTLALGVLASTGANFNNNEASAAAKPLDKSSSSLHH
                     GYSKVHVPYAITVNGTSQNILSSLTFNKNQNISYKDLEDRVKSVLKSDRGISDIDLRL
                     SKQAKYTVYFKNGTKKVIDLKAGIYTADLINTSEIKAININVDTKKQVEDKKKDKANY
                     QVPYTITVNGTSQNILSNLTFNKNQNISYKDLEDKVKSVLESNRGITDVDLRLSKQAK
                     YTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSIN
                     LNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKN
                     GGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSD
                     LKFTGDPRVGYKDISKKVKSVLKHDRGIGERELKYAKKATYTVHFKNGTKKVININSN
                     ISQLNLLYVQDIKKIDIDVKTGTKAKADSYVPYTIAVNGTSTPILSKLKISNKQLISY
                     KYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKD
                     IKKIDIDVKQYTKSKKNK"
     gene            2000..2314
                     /locus_tag="O2692_002014"
                     /pseudo
     CDS             2000..2314
                     /locus_tag="O2692_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
     gene            complement(2638..3924)
                     /locus_tag="O2692_002015"
     CDS             complement(2638..3924)
                     /locus_tag="O2692_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCZ4299636.1"
                     /translation="MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            4124..4222
                     /locus_tag="O2692_002016"
     CDS             4124..4222
                     /locus_tag="O2692_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299637.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
     gene            4465..4641
                     /locus_tag="O2692_002017"
     CDS             4465..4641
                     /locus_tag="O2692_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299638.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
     gene            4905..5285
                     /locus_tag="O2692_002018"
     CDS             4905..5285
                     /locus_tag="O2692_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCZ4299639.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            5282..6178
                     /gene="pmtA"
                     /locus_tag="O2692_002019"
     CDS             5282..6178
                     /gene="pmtA"
                     /locus_tag="O2692_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HCZ4299640.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            6179..6859
                     /gene="pmtB"
                     /locus_tag="O2692_002020"
     CDS             6179..6859
                     /gene="pmtB"
                     /locus_tag="O2692_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HCZ4299641.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINT
                     RKFKGGSR"
     gene            6856..7728
                     /gene="pmtC"
                     /locus_tag="O2692_002021"
     CDS             6856..7728
                     /gene="pmtC"
                     /locus_tag="O2692_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HCZ4299642.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            7728..8468
                     /gene="pmtD"
                     /locus_tag="O2692_002022"
     CDS             7728..8468
                     /gene="pmtD"
                     /locus_tag="O2692_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HCZ4299643.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(8534..9097)
                     /locus_tag="O2692_002023"
     CDS             complement(8534..9097)
                     /locus_tag="O2692_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCZ4299644.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            9218..9742
                     /locus_tag="O2692_002024"
     CDS             9218..9742
                     /locus_tag="O2692_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299645.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            9802..10359
                     /locus_tag="O2692_002025"
     CDS             9802..10359
                     /locus_tag="O2692_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCZ4299646.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            10356..11198
                     /locus_tag="O2692_002026"
     CDS             10356..11198
                     /locus_tag="O2692_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCZ4299647.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(11255..12295)
                     /locus_tag="O2692_002027"
     CDS             complement(11255..12295)
                     /locus_tag="O2692_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCZ4299648.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            12746..12919
                     /locus_tag="O2692_002028"
     CDS             12746..12919
                     /locus_tag="O2692_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299649.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
BASE COUNT         4664 a         1660 c         2142 g         4490 t
ORIGIN      
        1 aatcgacaaa tctaatcgga attaagaaat gaatgttgtt atgacattta agtttgaagc
       61 ttggtataaa caaaaccaaa caacatttta ggtattgtaa attaattagt taatcaaaat
      121 taaaagaact tttaacagtg ttaaaaaatt attgttaaag ataatttatt attaatattc
      181 agttaattca aaaaatagag aaagtctggc tataattaag ttgcaaatac gaattacaca
      241 aaaaaggaga gataatttat gaaatttaag tcattgatta caacaacttt agcattaggt
      301 gttttagcat caacaggtgc aaactttaat aataatgaag cgtctgccgc agctaagcca
      361 ttagataaat catcaagttc gttacaccat ggatattcta aagtccatgt tccatatgca
      421 atcactgtga acggtacaag ccaaaatatt ttatcaagct taacatttaa taagaatcaa
      481 aatattagtt ataaagattt agaggataga gttaaatcag ttttaaaatc agacagaggt
      541 attagtgata tagatttaag actatcgaag caagcgaaat atactgttta ctttaaaaat
      601 ggaacaaaga aagttatcga tttgaaagca ggtatttaca cagctgattt aattaataca
      661 agtgaaatta aagctattaa tattaacgta gatactaaaa agcaagttga agataaaaag
      721 aaagataaag caaattacca agttccatac acaatcactg tgaacggtac aagccaaaat
      781 attttatcaa acttaacatt taataagaat caaaatatta gttacaaaga tttagaggat
      841 aaagttaaat cagttttaga atcaaataga ggtattaccg atgttgattt aagattatcg
      901 aagcaagcga aatatacagt taattttaaa aatggaacga agaaagttat cgatttgaaa
      961 tcaggtattt acacagcgaa tttaataaat tcaagtgata ttaaaagtat caatattaac
     1021 gtagatacaa aaaaacatat cgaaaataaa gctaaaagaa actatcaagt tccatattca
     1081 attaatttaa atggtacatc tacaaacatt ttatcgaatc tttcattttc aaataaacct
     1141 tggacaaatt acaaaaattt aactagtcaa ataaaatcag tactgaagca tgatagaggt
     1201 attagtgaac aagatttaaa atatgctaag aaagcttatt atactgttta ttttaaaaat
     1261 ggtggtaaaa gaatcttaca gttgaattca aaaaattaca cagcaaactt agttcatgcg
     1321 aaagatgtta agagaattga aattactgtt aaaacaggaa ctaaagcgaa agcagacaga
     1381 tatgtaccat acacaattgc agtaaatggc acatcaacac caattttatc agatttaaaa
     1441 tttacaggtg acccacgtgt aggctacaaa gatatctcta aaaaagttaa atcagtattg
     1501 aagcatgata gaggtatcgg ggaacgtgaa ttaaaatatg caaaaaaagc aacttacaca
     1561 gtacatttta aaaatggaac gaaaaaagtg attaacataa attcaaatat tagccaactg
     1621 aatctgcttt atgttcaaga tattaaaaag atagatattg atgttaaaac aggaactaaa
     1681 gcgaaagcgg atagctatgt accatataca attgcagtaa atggcacatc aacaccaatt
     1741 ttatcaaaac ttaaaatttc gaataaacaa ttaattagtt acaaatattt aaatgacaaa
     1801 gtgaaatctg tattaaaaag tgaaagaggc atcagtgatc ttgacttaaa atttgcgaaa
     1861 caagcaaaat atacagtata tttcaaaaat ggaaagaaac aagtagtgaa tttaaaatca
     1921 gacatcttta cacctaattt atttagtgcc aaagatatta aaaagattga tattgatgta
     1981 aaacaataca ctaaatcaaa aaaaaataaa taaatctaat aatgtgaaat tcccagtaac
     2041 aataaataaa tttgaaaaca tagtttcaaa tgaatttgtg ttctataatg caagcaaaat
     2101 tacaattaat gatttaagta taaaacttaa atcagcaatg gcaaatgatc aagggataac
     2161 taaacatgac ataggacttg ctgaacgcgc agtgtataaa gtgtatttta aaaatggttc
     2221 gtcaaaatat gtagacttaa aaactgagta taaagatgaa agagtattta aagcaactga
     2281 cattaaaaag gtagatattg aacttaaatt ctaatcttta attttataat aaggcatctc
     2341 acaatcgtgg ggtgcctttt acatttgtag agatgtgatg cttgaatgtt tgaagtgatt
     2401 tgccaaaagt tatataaatt ggttctataa taaatcggac tgatagaaaa agttttttac
     2461 ttttcatctg tccgattttt tgattttgtt aggagagatg tataaaacta attaggtata
     2521 gtgtttgaca agttgctgaa taaaatatat cttatgatac tttgaaagaa ggaataattt
     2581 taaaaataaa aaaccaataa tccgagtcat actcatcaga ttattggttg aaattaatta
     2641 tcttaagtca tcaatcgctt tagcaattga atcaaataca tgtgggatat cttctttttc
     2701 tacacagcta aacgcaatac gaatatctgt tgcattaagt gcaataacac caattgaata
     2761 tttttcaatt aaatgtttac gaagttcttc agggtcaaca tcatgaactt ttattgccat
     2821 aaagtatcca gagttaaagt cataggcttg ccaatgtgaa tgatattgat cagcatagac
     2881 aacttcctta gtgacttcat agcgttcttt taatgtttga atattttgtt cgatttcttt
     2941 atcgaattgt ttattatttt ttaatacatg cttaacagcg ctttgtgttg gaagaggtcc
     3001 actagaaatg ttacttcgta taagaccttt tactttggct tctaatactt ctttagttgt
     3061 ttgatcagac gttccaaatg tcataaaccc aacacggaat ccccatgcga aaaattcttt
     3121 tgttgcacca tctaaacgaa caggtaatat tgcatttgaa tttaaattag ataatgcagt
     3181 aaataatgat tgagtataca catcttcata gaataaacca tagtatgcgt catccacaac
     3241 agctataact tttgtacctt tatcagctaa tgctttaatt gcatcgacga tggtagtcac
     3301 ttctttatgc gtaggtgtgt aacctgtcgg attattagga taatttaaaa tcataatgac
     3361 tttatcttta ttgtatgatt gtaaagcttc tacaagtgaa tcagtagtat aatgcccgtc
     3421 tttatcaaag ataggatatg tttgaaggtt tgcaccattt ctcgtattga aaacaagttt
     3481 gtaattaccc caattatgct ctggtaacaa gatagtgtca ccttgattta caaataaatc
     3541 gccaactaaa gataaaccat gtgttaatgc attcgtaaca attggtagtg acatgttgtc
     3601 gattgatagc tctggattgt cacgcaacat tttttgttgc cataaatcac gtaattcttc
     3661 gatgccttgt ggtggcgcat aagggaatat ttcatctgga gttaaatcat taaacattgc
     3721 atctaaagat gatgcaaaca tttttccgtc tttgtttgtc gccataccta tagttgcatt
     3781 atatgttgta ctctttgctt cagcagattg agataaaatg ccttttggat aaaacatatt
     3841 ttgacctaag tcagataaca ttgtaaaggc agttgcattt gactgttgaa gttgttcatt
     3901 taagctttgg gctaaaggat tcattattgc ttaacctctc tttaaaaaaa ttgtatgcct
     3961 atatattatc ttcaattata ccaaagataa agtgttgttg gtgaattaac gaataatatg
     4021 taaaatctgc tgccaaaata cgaacatatg ttctgttttt atgttagaat aggtgtaggt
     4081 catccttcag tatgatactt tagtcgaacg gaggtgtagt gtaatgatgt ggtggcaaga
     4141 agggatgatg acacttatta caggtggctt actaattatt tttcgacttt ggttagaact
     4201 gaaatggaaa aataaaaaat gatcctacac acataaataa ccccaagtct gctggtacca
     4261 gacttggggt tgtgtagtat aattctgtgg atacacttaa ataatatcat tgaacacaat
     4321 atatcgcaat caataatcat tttgattgaa tggttttgtt gcgctatttt agttgaaagt
     4381 gattagtacc ttgaactttt gaaattcaat aacttattaa aaaaatttat atttgttata
     4441 atgtcgatgt tgaggtgaaa taaattgaaa catctaacaa aaatatttgt agtacttgca
     4501 attattttat ttattattgg ttattattta caagcaacta atcatgaaag ccaaggtata
     4561 aaattattat tagcagcgat tatgtttatg atttgtgctt ttataagtag aagtaatgat
     4621 cgacgtaaaa atagtaaata gaattgactg tttaaaacga ttgtttatgt tgacgctacg
     4681 gttgtcacgt agagattaaa gacattcgtt ttagcagtct tttttattga agtagacaat
     4741 gcaagattaa atggtagtgt catttcattt tattttattc aactgctagt aatacaatac
     4801 tcagtgttat ttctgaagac tggttgataa tgagacatgc atatttacaa aactgtgtat
     4861 attgtgtata ttgtatatat acagaagttg atagggggac gttgatgaaa ataattttaa
     4921 aaaacaatag tgattttccg atttatgaac agattaagca acaagtaaaa caaaatattt
     4981 taaagggaca tgttgctcct ggagagcatt tgccgtcaat gagagaactt gccaaagatc
     5041 ttcaagtaag tttgattact accaaacgtg cttatgaaga tttagagaaa gacggttttg
     5101 ttacaacaat tagaggaaaa gggacctttg ttaaggagca agatagttct attttaaaag
     5161 agaaacaatt ttttaccatt gaaaatttgg ttaaagaatt ggttaatgaa gcgcaagcca
     5221 tcgaaatgtc acttgaggaa ctgcaggata ttttaacgtt catttatgag gaggaatcat
     5281 catgaatgcc atagaattaa gtaatgttaa ttattcaagt gatcagttta atttaaagaa
     5341 tatctctttc aaagttcctc aaggattcgt cactggtttt attggtagaa atggcgctgg
     5401 taaaaccaca ataataaggt taattatgga tttgtatcaa ccacaaactg gtgttattag
     5461 agtattagaa gaggatatgg ctctcaatcc gatagagtta aagaatagaa ttgggtttgt
     5521 ttactctgaa aattatttta atgagagatg gactacgaag caacttgaaa aaatgattgc
     5581 ccctttttat cgtaaatggg accatcaagt atttgaattt tatcttgaaa agttcgattt
     5641 accaatcaat aaatcaataa aaacattttc gactgggatg aaaatgaaat tatcactagc
     5701 agttgcattt tcacatcatg ctgaattgta tatttttgat gagcctactt caggtcttga
     5761 tcccttagct agaaatgagc tattggagat aattcagcaa gaattaattg atgaaaataa
     5821 aacaatattc atgtcgacac acattatctc agatttagaa aaaatagctg attatatcat
     5881 tcacttaagt gacggtgaag ttatcttaaa tggttcaaaa gaacaactgt tacaaagata
     5941 tcaagttgtg agtggtgcta ttgaggacct tgatgatgaa ttagcatctc tacttattta
     6001 tgaagaacat aagcgtacgg gatttatagg attaacagaa catgcgcaag tatttaaaga
     6061 aattttaggt cataaagtga atatcactac accttctatt gagaacttaa tggtttatct
     6121 tgaaaagaga aaacctaaat atcatgaaaa catcaaattg atggaagaag gtttttaaat
     6181 gaaggcattg ttaattagga attttaaatt acgacgatat acacttatca tctatgttct
     6241 attattaacc ttatatcctt tttatataat gcttgactca acaaagttct tttacttatt
     6301 gcaatctttt ataagtccaa caatattgat tatttggatt ttagatgctg gtcacttatt
     6361 tagactcaat cgtagattag gtggtaatga ttcatattat ttttatatga gtttaccagt
     6421 gtcaaaaaaa caattgctta atgccaacta tattacatgc attgttttaa cattaattgg
     6481 aacgcttgtt attagtttat atgcttatga agcagatgtg attgaaccaa attcaatcta
     6541 tttttcaact gcatatgcat ttgtcatatc taacttcttg tctataccaa ttgcatttag
     6601 tcaatttaca gaattgcgta gagtcaaagt gccatatggt atatacgtgt ttactattat
     6661 cattttagtt ccatttttat tttcaattgc aatagtattg gtgaattatt ttgttctaag
     6721 tcaatcatca ttcccagatt tatattcata tattttaaat attggttttc taattataag
     6781 cattgttata cttattgtta attattttaa acagctcaat aaaataaata ctagaaaatt
     6841 taaaggaggc agtcgatgaa attagaacat attacaaaaa aatacggctc aaatgtcgtt
     6901 ttaaatgata ttgattttga ctttggcgat agtagaattg tcggattaat aggaaaaaac
     6961 ggtgttggta aaacaaccgt tatgaaagta atgaatggta atattattaa atttgatgga
     7021 aaagtagata ttgataatgc agataatatc ggttttttaa ttgagcatcc taaattatat
     7081 gataataaat caggattgta taacttgaaa ttatttgcac aagtattagg taagggtttt
     7141 gataaagcat acacagacaa aattatagat gcatttggta tgagacctta tattaaaaag
     7201 aaagttaaga aatattcaat ggggatgaag caaaagttag caattgcagt atctttaatg
     7261 aataaaccta aatttttaat cttggatgag cctacaaatg gtatggatcc agatggctca
     7321 attgatgtgc tgactacaat taagtcttta gtaaatgaac ttgatatgag aattctaata
     7381 tcaagtcata agttagaaga tattgaatta atttgtgata gagctgtatt tttaagagac
     7441 ggacattttg ttcaagatgt aaacatggag gaaggtgttg catctgacac aacgatagtt
     7501 actgttgatc ataaagactt tgatagaact gaaaaatatc ttgcagagca tttccaatta
     7561 caaaatgtcg acaaagcaga cggacattta atgatcaatg cacaaaaaaa ttatcaagtt
     7621 atactaaaag cattatctga attagatatt tatccgaaat atattgaaac acgtaaaagt
     7681 tcattgcgtg atacgtactt caatataaat caaagaggtg ataaataatg agaattttaa
     7741 atttagttaa gtatgatttt tatagtatat ttaaaagtcc tttaacatat ttagcgatac
     7801 tagtcgtatc tagtttgatt gcaactcaaa gtatacttat ggcaaattcg atggataacc
     7861 cgaaacatat tattgtctat ggatctgtat ttgctgcagc aaaatggtta ttgttaataa
     7921 ttggattaat gtttgttgtt aagacaatta cgcgtgattt ttcacaaggt acaattcaac
     7981 tatatatgag taaagttaaa acacgcgttg gatacattat ttcgaaaaca atttcaatta
     8041 ttttaatttc aatattattt gcattaattc attatgtgat tttgattgtt gtgcaggcat
     8101 ctagtaatgg aaaaaatttg gcgttttcta aatatgtaga taatttatgg ttcttcctaa
     8161 tctttttact attctttggc ttgtttttat tcttaatcac acttgcatca caaaaaacag
     8221 caatgatatt ttcattaggt gtatttttag tactcattgt accgtttatt aaacctttta
     8281 ttacatttat cccaagatac ggtgaaaaag ttttagatgc ttttgattat atcccttttg
     8341 cttacttaac tgataaaatg attagctcta actttgattt tagcaattgg caatgggtaa
     8401 tttcattagg ttctatagtg atattcttca ttttgaatat cttatatgtc gctaaaaaag
     8461 acatttaata aaaataattt tgaggttggg aattttaaat tttcccaacc tcaaagtttg
     8521 tcttattgta aatttattta ttttctaatt tatttaggat ggaattataa actgctttcc
     8581 aaaatgaagc gtcagtttta tagcggtttg atataactaa gtgtgtttct ttttctaaat
     8641 ctgcatagtc tggatgatct ttgctcggta atttatcagc acgaacatca gttacaaatt
     8701 tttggacttc atttgctctt ggtccccaaa ctgtttcttg ttcgaattga tcattcaaga
     8761 atacgaagat aggaattgca cgtgatttac catttgttaa atattgatcg atcagttttg
     8821 tatcatcatc tctatggaac acgcgtactt ctaaatttaa tgcttcactg atgtgtttta
     8881 gaattgggag attcatcatt gcatctccac accagtcttc agtaattact aatactttag
     8941 aataattcat ctcttttatt tttttgatgc gtgaatcatc ttctggtaac tcaaatgatt
     9001 gatagatact gagaacggta tcttgatttg tcttcattcc atcaatgtat tcatttaagg
     9061 gttggctatt tttgaaataa gtttctaaat ttgtcattgt aaaaacctcc tttagcattt
     9121 acaacattat accaatttat agtaataaaa ggtaatgaaa taaattaatt gcaaattctt
     9181 tgttaatttt tgttaaggat gaaacgggaa gcaccttatg ctatatttaa ataagtacaa
     9241 agaaagggtg acatcagtgc gtattcaaaa tcgctgggtt gtgtttatat tatttttaat
     9301 ctgttctttt ggtgtattaa ttggtttata tcaatatcgt catacgaaaa ctgtggattt
     9361 gtctaatctt gaaataaacg atattaaatt aaatgagaag tttgataaaa aaggctatga
     9421 agtaaataaa aaaattaagt ttgatcgttt taaattttat aacagtaaag ctcaccctga
     9481 tcttaccgtt aaagtgagag aaaaggataa catcgttaag gggataatat tagtaagaga
     9541 tgaaaagata catactaatt ttgatggggg aattggttcg ccgataaata acgcgattga
     9601 aaatcttgga ttcggatata aaagaacaaa agttggcaat gatttctcat cggtaaagta
     9661 tattgataga gataaccatt taaaattaaa cttactttat caagatttag aaattaaacg
     9721 tattgaattt tttagtaaat agctttaggt cttaaagtta taaaaaacga atgaataatt
     9781 ttattgggat gagtgaccta tatgaacaag attacttatt taaatgaatt agaaacagca
     9841 cttgatgcgc tacctaggca tttaagagat caaaaaatgt atgaatatga acgttatttt
     9901 tatgagcaag aacttaatgg cgttgatgaa ggggaaattt taaagaagtt aaaagaccca
     9961 caagatgttg cagctgaaac aaaagctaga agtgtcattg attatgctga atctaaacca
    10021 acatttgaaa atatttcaag agctgttgct gcttcattaa gtttaggcat tctatctatt
    10081 tttgtcatcc ttataccagt atctatagtt ggattatttg tattagcatt atttttaata
    10141 tcacttttgc tgctgttttg tccaattatt ttattagcat cagcaatatc cagaggaatt
    10201 gtggactcaa ttagtaatgt attttttgcc atatcatatt caggattagg attagtattt
    10261 atcattgtca tatttaagat tttagaatac atttatcgtt taatcttaaa atatttactt
    10321 tggtatatta aaactgtcaa aggaagcgtt agaaaatgaa gaaattcttt tttattgggc
    10381 ttttagtgtt tgttgtcttt tttacagcag caaccattat ttggttcagc tatgataaaa
    10441 acaaatatgg tactaaacaa tatgataaaa cattcaaaga cgatgctttt gacaatgtat
    10501 ctataaattt ggatagtaca gaacttcgta taaaacgggg gaatcaattt agagttaaat
    10561 atgatggtga caatgatata ttaattaata tagtagataa gacgttgaag attagtgata
    10621 aaaggtctaa gacaagagga tatgcaattg atatgaatcc ttttcatgag aataagaaaa
    10681 cgttaacgat tgaaatgcct gataaaatga ttaaacgttt aaatctatca tctggagcag
    10741 gaagtgttag aatcagtgat gttgatttag agaacacaag tattcaaagc attaacggtg
    10801 aagtagttat taaaaactca aatcttgacg cactagattc aaagacaaat aatagttcta
    10861 cttatattag taaaagtaac attaaaaata gcaatattaa agttgttatt ggtacgctac
    10921 aaatcgacaa gagtcaaatt aaacaatcca tatttttaaa cgatcatggt gacattgaat
    10981 ttaaaaacat gccatcaaaa gtagatgcaa aagcttctac taaacaagga gatattcgtt
    11041 ttaagtatga tagtaaacct gaagacacta tactaaagct aaatccggga acgggtgata
    11101 gcgtagttaa aaataaaaca tttactaatg gtaaagttgg gaaaagcgac aatgttttag
    11161 aattttatac gattgatggt aatatcaaag ttgaataaat aaaggatgta agcaccgata
    11221 ttaggaagca taatttctct aatatcggtg ttatttattt gttggcaaaa gttaagtcgg
    11281 tatctatatt gccagtaaag tgagtgatat taaggtcttg accatctaac catgatttga
    11341 aatctattat ttctggtggc gcattttctc ccaatgtaaa atatgcagtt aatgtttcag
    11401 gttgatacat tgatgtatgg atggtgccag accagctttt gaatagttta ctgtaaattt
    11461 catactgagg attattgaat aacttaaatg ctgtagtcat atctaaatta tcattagttt
    11521 gtgaaatggt acgcgccagt ctttctttag attcttttgt ataattacga ttttcatgtg
    11581 ttaatatttc aaaatgattt gtacatatat tatcataacg aacatctatt gatctcggtg
    11641 tcacttcaac aattgcatgg ttcaatgatt tgtccatcag tatgtagcta aatgagcttc
    11701 tgtgtggtat ttctttcaat aattggattg cttctgttac atttcggcaa ttttcaagaa
    11761 ttagacgacc aatcatataa catacaaaac catttgctgg tttcttccgg tgcataaagt
    11821 tatagcccat agttaatcct gactcattca taccatccat tcttccagtt acccttgata
    11881 caggaccaat ttgagctaaa ccgctatctg taggttgata aagtaagtag cgaccatcat
    11941 aagttgcagg gtggtaatca taatttctaa ccatgaagtc tttgccttga aagaccgtgc
    12001 aaccactttc ttttaaatcg gtaaaacgat aatgtccaaa gtttaaaata atttggcgtg
    12061 ttggcatttt gagtatactt tgtagtccca ttaattcttc ccatatttga ggtgcgtatg
    12121 tttggaatat ttgataagtt tcatttacat ctatatcgaa acgtgggaca cgttttttcc
    12181 attctttttc tcgatttttt agaagaggtg tttgttgaag ccatttacca gttttaacac
    12241 ctaactcgaa atgtgaacct ctaaaagtca tgatatctga tgtcacttgt tgcatatcat
    12301 cggccccttt ctttttagtt gtaatatatt gtaaataaat agtaatcgta tgtatattga
    12361 atgtcatgtt aaataaagtt atattttact aaatgaaata taaaattgtt tgaggtgatt
    12421 tctcggtgta taagacttat caatcagtta aaacatattt ttatagatgg tggggatatt
    12481 gagttaaaaa cttaaaatca tcttatcata aatatcaatc ttaagttagc attcacgata
    12541 atagtcattg ttaacattag catataaggt catgtcacgt tgaaacagag gttcctcggc
    12601 atttttgaaa ataatcatta gcttgctcac tatataattt gatgaatata tttcgtgaaa
    12661 gtgggtattt atttaatgat tattctatat atgatagtat aatgaaatgt agataggtat
    12721 ttaatttaac agaggtgaaa ttgagatgtg gaattttatt aaatgtgtgt ttaaattcgt
    12781 atttagctta gttgctatta caacattagt tgctggtgtt ggtgtagtag catttgctta
    12841 tatctttaaa aaagattttg aagatattga aagaaaaact aaagaaatta tttctgatat
    12901 tgaaagtaaa aataactaat aacatttaga ggctgggaca taaatcccta aaaaac
//
LOCUS       DALAGF010000080        12460 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.080, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000080 DALAGF010000000
VERSION     DALAGF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12460
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.080"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(162..431)
                     /gene="rpsO"
                     /locus_tag="O2692_002029"
     CDS             complement(162..431)
                     /gene="rpsO"
                     /locus_tag="O2692_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCZ4299650.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
     gene            complement(546..1517)
                     /gene="ribF"
                     /locus_tag="O2692_002030"
     CDS             complement(546..1517)
                     /gene="ribF"
                     /locus_tag="O2692_002030"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCZ4299651.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            complement(1532..2449)
                     /gene="truB"
                     /locus_tag="O2692_002031"
     CDS             complement(1532..2449)
                     /gene="truB"
                     /locus_tag="O2692_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCZ4299652.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            complement(2618..2968)
                     /gene="rbfA"
                     /locus_tag="O2692_002032"
     CDS             complement(2618..2968)
                     /gene="rbfA"
                     /locus_tag="O2692_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCZ4299653.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            complement(3354..5471)
                     /gene="infB"
                     /locus_tag="O2692_002033"
     CDS             complement(3354..5471)
                     /gene="infB"
                     /locus_tag="O2692_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCZ4299654.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIIQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            complement(5476..5793)
                     /locus_tag="O2692_002034"
     CDS             complement(5476..5793)
                     /locus_tag="O2692_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HCZ4299655.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            complement(5790..6074)
                     /locus_tag="O2692_002035"
     CDS             complement(5790..6074)
                     /locus_tag="O2692_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCZ4299656.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            complement(6095..7270)
                     /gene="nusA"
                     /locus_tag="O2692_002036"
     CDS             complement(6095..7270)
                     /gene="nusA"
                     /locus_tag="O2692_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCZ4299657.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVNDVSVETNVEKESE"
     gene            complement(7291..7758)
                     /gene="rimP"
                     /locus_tag="O2692_002037"
     CDS             complement(7291..7758)
                     /gene="rimP"
                     /locus_tag="O2692_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCZ4299658.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            complement(8048..12358)
                     /locus_tag="O2692_002038"
     CDS             complement(8048..12358)
                     /locus_tag="O2692_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCZ4299659.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
BASE COUNT         3692 a         2462 c         1698 g         4608 t
ORIGIN      
        1 actctaggtg tacttatatc atctttatct cattgtaacc ttattattca ctcaatatta
       61 gcttaacata taataaatgt taatatttgt aaaaagcttt tttaatataa attaaggttg
      121 gaacataaat atgccccaac ctcaaagacg ttatattaag attaacgacg gataccaagt
      181 gatttaatta attcacggta acgttgaata tctttactac gtaagtagtt taataaatgt
      241 ctacgacgac ctaccatttt taataatcca cgacgtgaat ggtggtcttt tttgtgtgta
      301 cgtaagtgtt cgtttactgc gttgatttct gcagtaagta cagcgatttg tacttctggt
      361 gaaccagtat cagtttcgtg tacacggtat tctttaatga tttcgttttt acgttcttgt
      421 gaaattgcca taatcaattt cctccttaaa atgtttgaat actccttaat ccgagttagg
      481 cgttggagat tcgacctact aagaaaaagg tgtggtatag attgtttata taactatacg
      541 caactctaga tattataagc tacttcatca ccaaaatcaa ctgctaataa atatttagca
      601 cgcgatttat catcgttcat ttgtttaact aatgggtcga taccatcaaa tttaatctca
      661 ggacgtaaga aatgatgcca attcacggtc actcgttcac cataaatatt atcctcaaag
      721 tcaaagatat tcacttcgat gacaacttct gctttgttag gatcatgaaa tgttggcttt
      781 acacctatgt tagctacccc tcgatataat ttattttcag tgccgatttc aatactaaca
      841 gcataaacac ctttacgagg taacaaataa tcatcactag gttgaatgtt agctgttggg
      901 aagccaatag ttcttcccct tttttcacct tgcactacag tgcctttaat agaatatata
      961 tagcctaaag catcattcgc tttttgcaac tcaccattga ttaaatcttg acgaatagaa
     1021 gttgtagaaa ttttttcatt ttcaatttct tgtttactca caattgtcgt attaaacgca
     1081 tcatattctt gaagtacagt catattacct tttccaaatt taccaaaagt aaaatcaaaa
     1141 ccagcaatga cttcttttac attattttta attatataat tttcaacaaa atcttctacg
     1201 ctcacattag caaacctaga tgaaaaatta accactatac aataatcaat atcatgttgg
     1261 ctaatttttt cgattttatc tgaaagtggc gttaaatacg ttgttcgttt tcttttagga
     1321 ttcaacacga cagacggatg cggatcaaat gtcatcaccg ctttttttaa actgcgtgcc
     1381 tcagctattt cgtttaatat atcaaagact ttgtcatgac ctttatgcat gccatcgaaa
     1441 aatccgaatg ccattgcaac atcctctgta atatactgtt tagattgtat aggatgtgtc
     1501 acttctatga ctttcataaa ttctatctcc tttaattaaa gactttttta ggtttaattt
     1561 ctgattcttt tgtagggtgt accatataaa ttgctaatac tttttctgaa tcatcatcaa
     1621 taaatacaat ttggtcttta attttgttat caaattcatt tttattaaat ttctgcccat
     1681 ttaaaatacg tttttttatg tgcgaatctt taattttaat gcttggcaaa ccctttaatc
     1741 catattctaa aggaaacaat ttattttgca atgaatcctg ctcatgaagt tcttttattt
     1801 gttctaatgt aaggctatct ttcaacacaa atccaccaga ctcgattcgt gttaatttcg
     1861 acatatgtgc cggaaagcct aatttcacac caatatcagt tgctagcgtt ctaatatatg
     1921 tacctttacc acagatgacg cgtattttaa aatgacactc attttcttta aaatctaatt
     1981 cagatatacg cccaatgtct ttaatattaa ctttacgctt tggtctttca actgtttcat
     2041 tattacgcgc atattcatat aattttttac catttacttt gacggatgag tacatcggcg
     2101 gaatttgttc aatgatacct ttaaaacttt ctaacaatcg gtcaatatcg tccttattaa
     2161 aatctgctga gtgtacacct tttgtttcca atgtatcacc cgtttgatct tcagtcgttg
     2221 tacttcttcc tatcgataca gttgcttcat aagctttgcc catatccata acataatcac
     2281 taactctcgt tgcattacct atacataccg gtaacacgcc tgcaacttcg ggatcaagcg
     2341 tacccgtgtg acctattttt ttagttttta atattttacg caatttgaat acaacgtcat
     2401 gacttgttaa accgcgctct ttatatactg gtaatatccc attatacata tagtcacctt
     2461 cttttttaaa atatatttta atagcgtatc aactaaattt attcataaaa aaactggaaa
     2521 atgttaacta tttctacaca tctatgtatt aactgtttaa tgttaatata ccgatattaa
     2581 gaaattatca aacattttcc agatacctaa cactaaatta tctatcttgt ttgtgtaaat
     2641 cttgaatcat tcgttcaatt ttattaccat attcgattga ttgatcatat tcatacatta
     2701 attccggcat aatacgtaat cgcattctag aacctaattc agacttaatg aagccttttg
     2761 ctttatcaag tgctttaaat gtattttcta cttctttatc gttacctaat acagttaaaa
     2821 atactttagc ctgcgataaa tcatttgtta aaacaacatc tgtaattgta ataaaaccaa
     2881 ctcgaggatc tttgacttta ttgttgatga tatccattaa ttccttcttc atttgttcac
     2941 caacacgctc tgctctcata ctgctcattc ttgtcacctc tttatcttta agttcaattg
     3001 ttatttgcat tttttagtgt aacaatgctt aaacaattat tttagctcac acgttatttt
     3061 aataaattaa gcctataaaa tcaaataaag tttgccgtaa gataggcatt tcaacagaaa
     3121 attttccaat aatagtcgac attatatttt gtcattaaaa atcattctag gccaccacaa
     3181 aattttatac aaaatcatgt ccatataacg taacttttaa taggtaatct attctaataa
     3241 aaaaccaaaa gctagtatca aatatgctcc ctataaagta gacacttaaa aagtgcaata
     3301 tttataggga gcatatcaat cttaactata cttttagctt gtaatttatt taattaacgc
     3361 ttaatttcaa ccatttcaaa tgcttcgata acatcgcctt ctttaaggtc attgtagttt
     3421 tcaattgtaa taccacattc ataacctttt gcaacttcct tagcatcatc tttgaaacgt
     3481 ttaagtgtat ctaattcgcc ttcatattga acaataccat cacgaataat acgtacacca
     3541 gcatttcgcg taattttacc ttcagtaaca taacatccag caatagtacc aactttagaa
     3601 actttgaatg tttgacgaac ttcagcttgt ccgataactt gttcttcaaa ttctggatca
     3661 agtaaacctt tcatcgctga ttcaatttct tcgataacat tataaataac tctgtgtaaa
     3721 cgcatatcaa cattttcagc ttctgcagca cgttttgcac cactgtctgg acgaacattg
     3781 aaaccaatga taataccatt tgaggcatta gcaagtgtca cgtctgactc attaattgca
     3841 ccaaccgctg tatgaatgat acgaacattt acgccttcaa catcaatttt cattaatgat
     3901 gcagctaaag cttcaacaga accttgaaca tcacctttaa taataacgtt taaatctttc
     3961 atttcacctt gtttcatttg ttcaaacagg ttatctaatg aaacattttt actttcttga
     4021 cgttgttgta taatgctagc ttcgtgtctt gattcaccaa tacgacgagc ttgtttttca
     4081 tcactaaata caacaaagcg atccccagct tgtggcacat cattaatacc tgtaatttca
     4141 acaggcgttg atggaccagc cgttttgatt ctttgaccta agtcattaac cattgcacgg
     4201 atacggccgt aagtattacc aactacaatc gcatcaccaa catttaatgt accgttttgt
     4261 actaataatg atgcagaagg accacgtgat ttatctaatt cagcttcgat aactgtacca
     4321 acagcacggt ttttaggatt tgctttaagt tcttgaactt ctgcaactaa tcctatcatt
     4381 tctaataaat cgtcgatacc atcaccactt aatgcagaaa gtggaacgaa aattgtttcg
     4441 ccgccccaat cttcaggaat taaaccatat tcagttaatt cttgcataac tcgatcagga
     4501 tttgaagttg gtttatcaat tttatttact gcaacaataa ttggtacttc tgcttcttta
     4561 gcatggttaa ttgcttcaat tgtttgtggc ataacaccat cgtcagctgc tactactaaa
     4621 atagtaatat ctgttacttg tgcaccacgc gcacgcatcg ttgtaaatgc agcatgtccc
     4681 ggtgtatcta agaaagtgat ttttttgcca tcgttttcaa tttgatatgc accaatatgt
     4741 tgagtgattc cgcctgcttc acctgctgta acttttgtat gacgaattga atctaataaa
     4801 gtcgttttac catggtcaac atgtcccata attgttacaa ctgctggtct ctcaattgcc
     4861 tctggatcat ctttttcgtc ttcgaaatag attgataagt cttcttcatt aatcacaact
     4921 tcttcttcaa cctcaacgcc ataatcatcg gcaattaatt cgattgtttc ttgatttaat
     4981 gattgattga tattagcaac aataccaagt aagaataatt ttttgataat ttctgatgat
     5041 tcaacattta atttatccgc aaattcgcct actgtaatac cttcttgata tgtcactttt
     5101 gatggtattt cttttggagc agctggttga ttttgtggtt tattattctt gttatttttc
     5161 ttattatttc tattattctt tttattgcct ttgttgcctt tattattttg ttgttgattc
     5221 tttttgttat ctttttgttg ccctttattt tggttttgat tgtttgattt ttggtgatta
     5281 ttttgagtgc tttgtttatt gtcgttcttt tgttcttttt tgaacttttt atctaatgct
     5341 ttaatttggt catcttccaa agcttgcata tgatttgaaa cctcaatatt catgcttttt
     5401 aactcatcta taatctcttt actctttaga tttaattctt tcgcatattc gtaaattctt
     5461 tgtttactca tataatcact ccttatgata ttcatctatc attgatagca actttttagc
     5521 aaagcctggg tcagtaatcc ctacattaac acgctcacct tttccaagtg ctattcccaa
     5581 ttcatttcga tttccaaact ttctgaatgg aactttgtaa ctcttacatt tatctgtaat
     5641 taatttagct gtattatcag acgcatcatt tgcaacaata acgagcttca aatttccttt
     5701 tttaatctca ttaacaatga ctgattcacc tgtttttact ttaccagctc tcattgctaa
     5761 tcctaaaaag tttaatattt gatctatact cattttggga tctcttctct ataaattaat
     5821 ctaataattt ctttgtaaac aggatccaat tgctctttag atgctttaaa atatttttct
     5881 aaaatttctt tttgttgtgc tttttcaacc atagcaacat ctttagaaac atatgcgcca
     5941 cggccttgtt tctttccagt aacatccgca aagatttcgc cttctttatt aacaacaaca
     6001 cgaatcatat cttttttggg atgcatttca tttgaaagaa tacattttcg catcggaatt
     6061 tttttctttt tcatagatac ttcactccaa cctattattc agattctttc tctacatttg
     6121 tttcaactga aacatcattt acctcttcgg ttgattctgt tgtgtcagca tcttctaaag
     6181 caacatcttc ttcagttact ttttcagctt caactactgg atagataccc gcttcacgcg
     6241 catctgtttc tgatttaata tcaattttcc agccggttaa tttagcagct agacgtgcgt
     6301 tttgtcctct tttaccaatc gctaatgaca attgataatc aggaacaaca actactgtag
     6361 attgatttgt ttcatcaaca ataacttcta aaacttgaga agggcttaaa gcatttttta
     6421 caaatacttt tggatcttca ttccattgaa cgatgtcgat tttttcacca cctagctctt
     6481 caacaacagc ttcaacacgt gcgcctttag caccaacaca tgcaccaaca gcatctatat
     6541 cattgttttc agagaagaca ctaattttag agcgatcgcc agcttcacgt gctactgatt
     6601 taacaattac agtaccatcg taaatttctg gaacttcttg ttcaaataaa cgttttaata
     6661 aacctggatg gctacgagaa acatagattt gaggaccttt tgtcgtttgt tccactttgt
     6721 taacatatac tttgatacgt tcgttaggaa tatatttttc gttaggactt ctttctgctt
     6781 cagataaaac agcttcgata cgacctaaat tcacatatac ataacgatgg tcaacacggt
     6841 caataattcc agtaagtatg tcttcttctt tgtctataaa ttcttcaaat aaaatttcac
     6901 gttcagcatc acgaagacgt tgcattactg cttgtttcgc tgcttgagca cctacacgac
     6961 caaaatcttt aggtgttaca tcttcttcgt atatatcacc aatttcatat gcagggtttt
     7021 taacaagcgc tgtacttaaa tccacttcat ctctgtcgtc aaatacttct tcaacaacat
     7081 ctttacgagc gataactttg aaagtacctt gatccatatt taattccaca cggacatttc
     7141 ttgcactatc ataattcttt ttgtatgcag taattaaagc tgcttcaata gcatcaatta
     7201 atactgctct aggaatcttc ttttcttttt ctaaatactc agtagctaat aataattcat
     7261 tacttgacac gttttttcct cctcatcacg ttaaatcata actgcgtgac gtgcttttgc
     7321 tattttgtct ctcggtatct ctatatcttt cgttcttgct ttgattttaa cttgtactac
     7381 aattgtttca ttattgactt cttgtaaaat gcctaaccat tccttttcac cttcaattgg
     7441 tacatataaa gaaacaaata caggtttagt tattgcattt tggaaatctt gttctttttt
     7501 aattggacgt tctgcaccag gtgacgctac gtctaaataa tacatttcag gaataggatc
     7561 atttgcatcc atagcttcac ttattttttc agaagctagc gtacaatcat ttaaatctac
     7621 gccaccttct ttatcaatag agattctaag aaaatgatct ctaccctctt tgacatattc
     7681 aacgtctaca agttcaaaat tcaagtcttc cataattggt ttaacaatca cttctacttg
     7741 ttctgtaatt ttactcatac aggcctccct ttttggcaaa tagaaaagag cgggaatctc
     7801 ccactcttct gcctgagttc actaattttt aagcaactta attatagcat aagtttatgc
     7861 ttgaaacaaa tgacttcact attaatcaga gattcttgta aaagtttgtt cctttatttc
     7921 accattacat ttgaatagac tcgtaagaca ttgaaaagaa atacgggcat aattttgtgt
     7981 ccagcaacaa ttttgtattt ctttgactac gccatacgca ttaaataaat accttgatta
     8041 tttcatttta catatcaaat atcgaaagtt gagctttatc tggtaaattc ggtaatgagc
     8101 ctaactcatc taaatactca ataattttct gagataatcc agcttttttg tttaaatctt
     8161 cttttgataa aaatgggcca tcgtcacgag cttcaacaat tcgtttcgca acgttttcgc
     8221 caagcccagg cactgatatg aacggcggaa taagtgtatc gccttcaatg ataaattcga
     8281 acgcctgact cttttctaaa ctaatcggtt gcattcgata acctcgatgc gccatttcat
     8341 tcataatttc caagactgtt aatacgtctt tttctttttt acctagatcc atatagcgag
     8401 aatacatgtc ttttacagta tttcgaatgc ttgttttatc tttaatcatc gtgattaaat
     8461 caaagtctga cgcacgaatt gtaaagtaag atgcatagta ataaagtgga tgatgtactt
     8521 tgaaatatgc gatacgtact gccattaaaa cgtatgctgc tgcatgggct ttagggaaca
     8581 tgtacttaat tttaagacat gaatctaaat accaatctgg cacttcattt tctttcatcg
     8641 tttcaatcat ttcttcagtt aaacctttac ctttacgtac tgactccatt attttaaaag
     8701 ccattgatgg ttctaaacca gcatacatta aataaaccat gatatcatca cgacaaccaa
     8761 ttacacttga taaatcacat ataccggttt taattaattc ttgagcattg cctaaccaca
     8821 catctgtacc atgagataat cctgagattt gaactaattc agaaaatgtt gttggctttg
     8881 tatcttctaa catttgacgc acgaatcctg taccgaattc tggtacccca aatgtacctg
     8941 ttttacataa aatttcatct tcagtaacac ccaaactttc aggtgtacta aatatctgca
     9001 taacttcttt atcatctaca ggtattgttt ttggatcaat tcctgataaa tcttgaagca
     9061 tacgaatcat tgttggatca tcgtgtccaa gtatatcaag ttttaataca ttatcatgaa
     9121 tagaatggaa atcaaaatgt gtcgtcatcc atgctgaatt ttgatcatcg gcaggatatt
     9181 gtatcggcgt aaaatcataa atatccatgt aatcaggtac tacaataata ccccctggat
     9241 gctgtccagt tgtacgttta acacctgtac atcctttaac gagtcgatct atttcagcac
     9301 ctcttttgtg gataccttga tcattcaaat aacctttaac ataaccaaaa gcagtctttt
     9361 cagcaacagt accaattgta cctgcacgga atactttatc ctcaccaaat aatacttttg
     9421 tgtagttatg ggcattcggt tgatattcac cactaaagtt taagtcgata tcaggaactt
     9481 tatctccctt aaatcctaaa aatgtttcaa acggaatatc ttgtccttct ttaataagtg
     9541 gcgctccaca agtttcacac gtcttatcag gtaaatcaaa tcctgatcct actgaaccat
     9601 cattgaaaaa ttcactcgtt ttacagttcg gacaaatata gtgtggcggt aacgggttta
     9661 cttcagtaat ctcagtcatt gtcgctacaa aactagaacc tactgaacca cgggaaccaa
     9721 ctaagtatcc atcatctaat gattttttaa ctaaacgttg cgaaattaag taaattaccg
     9781 caaatccatt accgataata ctttttaatt ctttttctaa tcgatcaatt acgatttgag
     9841 gcaggtcttc accatacagt ttacgcgcat ttgcataact tagttctcta atttcttcgt
     9901 tagcaccttc catacgcggt gtgtataatt catctttaat aggaacaaca cgttcaattc
     9961 gatctgctaa ttcgtttgta tttttcacaa caatttcatg cgctttttct tcacctaaaa
    10021 aatgaaactc gtttaacatt tcatctgtag ttctaaaatg tgcttccggt aaagttgagc
    10081 gattaagtgg attgccgggt tgtgatgcta ttaaaatttt acgtgcgata ccatcatgtt
    10141 caaacaaata gtgtgcattt cctgtcgcaa taacaggtat acccgctgtg tcacctgcat
    10201 gtattaaacg ttgataaatt tcatgtaatg tttcagtatc tctaataagc tctctatcaa
    10261 ttaaatcttg ataaagtgcc ggtggttgaa tttcaataaa atcataatat ttggcaattt
    10321 tttcaacttg actctggtcc ttctgcataa ctgccgtaaa taattcacct tcatcacacg
    10381 ctgtacctac caataatccc tcacgatatt catctaacaa tgaacgtgga attcgaggtg
    10441 tacggtagaa atacttcacc aatgatgcac ttacaatttt aaatagattt ttaagacctt
    10501 gttggttttg tacaattaat gtgacatgac taggtcttgc acgtttatat gcatcttcat
    10561 tactgagttt tttgttgatt tcgttatgat ttaatacgcc taattctttc atttgttgaa
    10621 ccatttttat gaaaatgtaa gctgttgctt ctgtatcata aatggcacgg tgatgttgcg
    10681 ttaattctac gccatatttt ttagccaaga aattcaaacc atgtttacca tattcagtat
    10741 taatcgtacg agataattct aaagtatcga taacaccatt cgttgatggt ccaaacccaa
    10801 gacgttcata tcccgtatcg atgaagccca tatcaaacga agcattatgc gctacgaata
    10861 tcgcatcgcc aacccattct ttaaactctg taagtacttc ttcaatctca ggggcatcta
    10921 ctaacatatc atcagtaata tgcgtcaaat tgataatcgt ttccgataat cgttcatgcg
    10981 gattactaaa cctttcaaac ttatcgatga tttcaccgtt atgaactttc acagctgcaa
    11041 gctcgatgat tttatcatac tgatttgata aaccagttgt ctcaacgtcg aacacaacat
    11101 aagtagcatc ttttaatacg acatcttgtg gtttgtatgc aatcggaaca ccatcatcaa
    11161 ctaacatacc ttccataccg tatatcattt taatgccatg tttttccgct gctgcgtgag
    11221 catctggaaa tgcttgcaca acattatggt ctgtaaccgc aatggctgga tgtccccagt
    11281 ctgctgcctg tttaacatac gcaccaatat tgggtatacc atccatttgg ctcattgcag
    11341 tatgcaagtg gaattctaca cgcttttctt cagccttatc tttttttgtc gcttttttaa
    11401 tctcttcaat atcagacatc atcataacta aatctctaat aaatgtatct tcttcaatac
    11461 gaccttgagc cctaacccat ttaccaacac ttagcgcttt aaaatgttct aaatcatctt
    11521 tgtttttacg agtaaacatt tttaaaacta aagagtccgt atagtcagtc actttaattt
    11581 ctacgatatg gcgaccactt ttaagttctt ttaagtttat atcaaaaatg acaccctcta
    11641 ttgcaacttt aaactcttcc tcaataatag attcaattgg tttaatattt tcaatttgaa
    11701 tcggcttacc aatttgacac ttatcgacag cactttcgtt gttatcttgt tgtttcgctt
    11761 tttcagcttt cattttttca agtttctctg ttgccaatcg tgcactttgt tcgtcttctt
    11821 cttgaatatg tgcttctaaa gaagctaagt tttgttcttg atcattatca tttgtttcga
    11881 atatgatttt atcgatatca aaaccacaat ttctaaacgc tttgataaga cttccattac
    11941 atgccttatc aaaatgatta cgttcaatgt catttgatac cattactttt aatacttttc
    12001 cagacataat aagctttttc tgtttcaatt gacctttaac ttttggagat aaagctgttt
    12061 ggtcaataca gtgcccaaag tatttaattg catgttcatc ttgattcgtg ccatttgtta
    12121 ccgtaaaacg acatgtaacg ttggcgatat ctttaaactc ttgctctatt gcatttataa
    12181 ataataaata atcttcatga gctaagaatt gtggtaatgt aatatgaaat tcccatgttc
    12241 tgtttttgtt agaaacatct atacgtgtca gttcacctga atttaaaatt tcagcatcta
    12301 attgatttga aattttaatt tgatcagcaa gcactttaaa tttttgttgc tctgtcattg
    12361 ccaagacaat taccacctta aatagaaatt acttttacgt gttcgaacaa tatttagatt
    12421 atgtgagcat agtaatgata gaaaattaaa tgctttatat
//
LOCUS       DALAGF010000081        11812 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.081, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000081 DALAGF010000000
VERSION     DALAGF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11812
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.081"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            80..1066
                     /locus_tag="O2692_002039"
     CDS             80..1066
                     /locus_tag="O2692_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HCZ4299660.1"
                     /translation="MKKCIKTLFLSIILVVMSGWYHSAHASDSLSKSPENWMSKLDDG
                     KHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYTWPGYDDIVSEIIDSNK"
     gene            1287..2057
                     /locus_tag="O2692_002040"
     CDS             1287..2057
                     /locus_tag="O2692_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ4299661.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAEVKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTT
                     NGYYYVDVTKDEDEGKPHDNEKRYPVKMVDNKIIPTKEIKDKNIKKEIENFKFFVQYG
                     NFKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTSKAPKLLLKGSG
                     NLKGSSVGYKDIEFTFVEKKEENIYFSDSLDYKKSGDV"
     gene            2109..2879
                     /locus_tag="O2692_002041"
     CDS             2109..2879
                     /locus_tag="O2692_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ4299662.1"
                     /translation="MIKRVNKLVLGISLLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVNSQMAIQNKGEALKIKGMLLKIDRNTRSA
                     KGFYYTNEIKTEKYEVAQDNQKKYPVKMINNKFISTEEVKEENIKKEIENFKFFAQYS
                     NFKDLMNYKDGDISYNPEVPSYSAQYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKKIEFTFLENKNENIYFTDSLHLEPSEDK"
     gene            2943..3713
                     /locus_tag="O2692_002042"
     CDS             2943..3713
                     /locus_tag="O2692_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ4299663.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAKIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMATQNKGEALKVKGMVLYMNRNTKTT
                     KGYYYVNAIKNDKDGRPQENEKRYPVKMVDNKIIPTKEIKDKNIKKEIENFKFFVQYG
                     NFKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKDIEFTFVEKKEENIYFSDGLIFKPSEDK"
     gene            3780..4547
                     /locus_tag="O2692_002043"
     CDS             3780..4547
                     /locus_tag="O2692_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ4299664.1"
                     /translation="MKRLNKLVLYISFLILVISFTAGCGIGKEAEVKKSFEKTLSMYP
                     IKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMVVQPKGERMKSKGMVLYMNRNTKTTT
                     GKYIVSETLHDEDGRPKSKDKEYPVKMVDNKIIPTKGIKDENIKKEIENFKFFAQYGS
                     FKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYKIPTNKAPKLLLKGSGD
                     LKGSSVGYKDIEFTFVEKKGENTFFTDSLHLEPSEDK"
     gene            4641..6878
                     /locus_tag="O2692_002044"
     CDS             4641..6878
                     /locus_tag="O2692_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ4299665.1"
                     /translation="MQRDYLIRVETESMSDFKRLNSLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDVHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFHHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHDYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFQPTNHQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVIPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLDQYDSRDYIDAFKIFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHRLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCKNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            7029..8207
                     /locus_tag="O2692_002045"
     CDS             7029..8207
                     /locus_tag="O2692_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCZ4299666.1"
                     /translation="MNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKTMIENGVLDGVDHV
                     LGVHVMSTMKTGKVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEMCEPQPPSED
                     FAYYAKERPSAFIYTGAAVENGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            8209..9597
                     /locus_tag="O2692_002046"
     CDS             8209..9597
                     /locus_tag="O2692_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4299667.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGTDM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRVIQGVSAACIMPSTLAIMKTYYQGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEITNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(10090..11751)
                     /locus_tag="O2692_002047"
     CDS             complement(10090..11751)
                     /locus_tag="O2692_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCZ4299668.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDLWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
BASE COUNT         4268 a         1666 c         2215 g         3663 t
ORIGIN      
        1 aagttttaaa attttacaca tatttttatt taaaaaagat gtataattaa tgtattaaat
       61 atagaaagaa gttgatatta tgaaaaagtg tattaagact ttgtttttaa gtatcatttt
      121 agtagtgatg agtggttggt atcattcagc acatgcgtca gattcgttga gtaaaagtcc
      181 agaaaattgg atgagtaaac ttgatgatgg aaaacattta actgagatta atataccggg
      241 ttcacatgat agtggctcat tcactttaaa ggatccagta aaatcagttt gggcaaagac
      301 tcaagataaa gattacctta cccaaatgaa gtcgggagtc aggttttttg atattagagg
      361 tagagcaagt gctgataata tgatttcagt tcatcacggc atggtttatt tgcatcatga
      421 attaggaaaa tttctcgatg atgctaaata ttacttgagt gcttatccaa acgaaacaat
      481 tgtgatgtct atgaaaaagg actacgatag cgattctaaa gttacgaaga catttgaaga
      541 aatttttaga gaatattatt ataataaccc gcaatatcag aatctttttt acacaggaag
      601 taatgcgaat cctactttaa aagaaacgaa aggtaaaatt gtcctattca atagaatggg
      661 gggtacgtac ataaaaagtg gttatggtgc tgacacgtca ggtattcaat gggcagacaa
      721 tgcgacattt gaaacgaaaa ttaataatgg tagcttaaat ttaaaagtac aagatgagta
      781 taaagattac tatgataaaa aagttgaagc tgttaaaaat ttattggcta aagctaaaac
      841 ggatagtaac aaagacaatg tatatgtgaa tttcttgagt gtagcgtctg gaggcagcgc
      901 atttaatagt acttataact atgcatcaca tataaatcct gaaattgcaa aaacgattaa
      961 agcaaatggg aaagctagaa cgggttggct gattgttgac tatgcaggat atacgtggcc
     1021 tggatatgat gatatcgtaa gtgaaattat agatagtaat aaataaggat tcaataatga
     1081 tattaagacg agtatgaaaa tagttagatt ctaattattt tcactactcg tttttatttt
     1141 gaaaataagt aataattcaa caatattata aattgaacag attgtttgtg aaatttttga
     1201 taatattaaa gtgaaaaagt gttataaatt gataaatata tgtaattaac aaaaacaaat
     1261 cattttaaaa agaagagagt tgtaagatga tgaaacgatt aaacaaatta gtgttaggca
     1321 ttatttttct gtttttagtc attagtatca ctgctggttg tggcataggt aaagaagcgg
     1381 aagttaagaa aagctttgaa aaaacattga gtatgtaccc tattaaaaat ctagaggatt
     1441 tatacgataa ggaaggctat cgtgatgatc agtttgataa aaatgataaa ggtacatgga
     1501 ttataaattc tgaaatggtt attcaaccta ataatgaaga tatggtagct aaaggcatgg
     1561 ttctatatat gaatagaaat accaaaacaa caaatggtta ctactatgtc gatgtgacta
     1621 aggacgagga tgaaggaaaa ccgcacgaca atgaaaaaag atatccggtt aaaatggtcg
     1681 ataataaaat cattccaaca aaagaaatta aagataaaaa cataaaaaaa gaaatcgaaa
     1741 actttaagtt ctttgttcaa tatggaaact ttaaagattt gtcgaagtat aaagacggag
     1801 atatttcata taatccagag gtgccgagtt attcggctaa atatcaatta actaatgatg
     1861 attataatgt aaaacaatta cgcaaaagat atgatatacc gacgagtaaa gctccaaagt
     1921 tattgttaaa aggttcaggg aatttaaaag gctcatcagt tggatataaa gatattgaat
     1981 ttacgtttgt agagaaaaaa gaagaaaata tatactttag tgatagctta gattataaaa
     2041 aaagcggaga tgtataatca tggctcaatc agaatatgaa atcaatcccg gaaaaagaga
     2101 gtgatgaaat gataaaacgt gtaaataaat tagtgcttgg tattagtctt ctgtttttag
     2161 tcattagtat cactgctggt tgtggcatgg gtaaagaagc ggaaataaag aaaagttttg
     2221 aaaaaacatt gagtatgtat ccgattaaaa atctagagga tttatacgat aaagaaggat
     2281 atcgtgatga tcaatttgat aaaaatgata agggaacatg gattgtaaat tctcaaatgg
     2341 caattcaaaa taaaggagaa gctctaaaaa taaaaggcat gcttttgaag atagatagaa
     2401 atacaagaag tgcaaaagga ttttactata ctaatgaaat aaagacggag aaatacgaag
     2461 tagctcagga taatcaaaaa aaatatccag ttaaaatgat taataataaa ttcatttcta
     2521 ctgaggaagt taaagaagaa aacataaaaa aagaaatcga aaactttaag ttttttgcgc
     2581 aatatagcaa ttttaaagat ttaatgaatt ataaagatgg agatatatca tataatccag
     2641 aggtgccgag ttattcagct caatatcaat taactaatga tgattataat gtaaaacaat
     2701 tacgtaaaag atatgacata ccaacaaata aagcgccgaa gctgttgttg aaaggtacag
     2761 ggaatttaaa aggttcatca gttggatata aaaaaattga atttactttt ttagagaata
     2821 aaaatgaaaa tatttacttt actgatagtc tacatcttga accgagcgag gataaataat
     2881 catcttttaa ccagaatatg aaatcaatct aggacaacct aataaaagga gagagttgta
     2941 agatgatgaa acgattaaac aaattagtgt taggcattat ttttctgttt ttagtcatta
     3001 gtatcactgc tggttgtggc ataggtaaag aagcgaaaat aaagaaaagt tttgagaaga
     3061 cattgagtat gtatccgatt aaaaatctag aggatttata cgataaagaa ggttatcgtg
     3121 atgacgaatt tgataaaaat gataaaggta catggattat tggttctgaa atggcaactc
     3181 aaaataaggg ggaagctctg aaagttaaag gtatggtctt gtatatgaat agaaatacca
     3241 aaacaacaaa aggatattat tatgtaaatg caataaagaa tgataaagac ggaagacccc
     3301 aagagaatga aaaaaggtac ccagttaaaa tggtcgataa taaaatcatt ccaacaaaag
     3361 aaattaaaga taaaaacata aaaaaagaaa tcgaaaactt taagttcttt gttcaatatg
     3421 gaaactttaa agatttgtcg aagtataaag acggagatat ttcatataat ccagaggtgc
     3481 cgagttattc ggctaaatat caattaacta atgatgatta caatgtaaag caattacgta
     3541 aaagatatga cataccaaca aataaagcac cgaagttgtt gttgaagggt acagggaatt
     3601 taaaaggttc atcagttgga tataaagaca ttgaatttac gtttgtagag aaaaaagaag
     3661 agaacattta ctttagtgat gggttaattt ttaaaccaag tgaggataaa taatcatgac
     3721 tcgatcaaaa tatgaaatca atccaggaaa gataataaga agaataggat tgtgaaatta
     3781 tgaaacgatt aaacaaattg gtattgtata ttagtttttt gatattagtc attagtttca
     3841 ctgctggttg tggcataggt aaagaagcgg aagttaagaa aagctttgaa aaaacattga
     3901 gtatgtatcc gattaaaaat ctagaggatt tatacgataa agaaggttat cgtgatgacg
     3961 aatttgataa aaatgataaa ggtacatgga ttattggttc tgaaatggtt gttcaaccta
     4021 aaggggaacg tatgaaatca aaaggtatgg ttctatatat gaatagaaac actaagacta
     4081 caaccgggaa gtatatagtg agtgaaacac ttcatgatga agatggtaga cctaagagta
     4141 aagataaaga atatcccgtt aaaatggtag acaataaaat cattccaact aaaggtatca
     4201 aagacgaaaa tataaaaaaa gaaatcgaga attttaagtt cttcgcgcaa tatggcagct
     4261 ttaaagattt gtcgaagtac aaagacggcg atatatcata taatccagag gtaccgagtt
     4321 attcagcaaa atatcaatta actaatgatg actataatgt aaaacaatta cgaaaaagat
     4381 ataaaatacc aacgaataaa gcaccaaagt tattgttgaa aggttcggga gacttaaaag
     4441 gttcatcagt tggatataaa gacattgaat ttacttttgt ggagaaaaaa ggtgaaaata
     4501 ctttttttac tgatagtcta catcttgaac cgagtgagga taaataatca tccacacaca
     4561 cgattcaata tgaaattcaa atatgttgct gtaaaataat gtaaaataaa cgtattcata
     4621 ttactgaatt gagggatagt atgcaacgtg attatttaat tcgagtagaa actgagagta
     4681 tgtcagattt caaaaggctc aatagtttaa tgattggttt tgttattaaa ggtgaggcac
     4741 acatttatga tgaaaataat atgacgcaat gcaacagtgg cgacattttc atcattaacc
     4801 accgcgactt gtatcgattt caacttcaac aagatggcat catatgttat atccaattcc
     4861 aaatgaaata tttagcagac aagtttgatg atgtgcattg tctatatttt cacttaacag
     4921 atgcgaccac aacaaagaat atacatcaac tgagaaatat aatggcaaga ctggtttcaa
     4981 cacatatccg acataatgag ttgtctaaat tgactgagca acaacttgtg attcagttgc
     5041 ttatgcatat gattcattat gtcccgcgta catatcattc gaaccaaagt atcttaaatg
     5101 atgataaagt gaatcaagta tgcgactata tcgagttaca ttttcatgaa gatttaagcc
     5161 tttcagaatt aagcgaatac gttgggtggt cagagagcca tctgtctaaa aagtttacag
     5221 aatcgctagg tgtaggattc catcatttct taaatacgac gcgaattgag catgcgaaac
     5281 tcgatttgac atacacagat gaaaccatta ctgatattgc attgcaaaat ggcttttcaa
     5341 gtgcagcgag ctttgcgaga acatttaaac actttacgca tcaaacacct aaacaatatc
     5401 gaggtgatcg tccagcaatc actgaaaacc agcaatcggc acaacatgat tatcacgacc
     5461 gtgaattgat attactttta aatgactaca ttgaagaaat gaatcatttt attgaagata
     5521 ttgaaaagat gaactataaa gagattgcct ttcaaccaac caatcaccaa ctaaatcaat
     5581 ttaatcatat tattcaagtg ggctatttga ggaatttgct caatacacag tatcaatcac
     5641 agttgcttac atgtcatcat gattttcaag tcaatgaagt attagcatat gatgtgattc
     5701 catatatcat gaaaaagctc aatgcgccat tcacgtatga tgcagaaatt tcgaatatat
     5761 tttatgatat tgatttgtgt ttagactttt tattagatca taactttagt ttgaccatgc
     5821 atttggatca gtatgactca cgtgattata tcgatgcatt taaaattttt atccatcacg
     5881 ttgccctgca tgtcagtcat agaaaagatt tgaagttcaa cttgtatgtg acgacattgc
     5941 acacgtcttt gattgaaatg attgattatt ttaaagcatt attccctaat ggtggcttgt
     6001 acattcactt agatcaagct acggaaagac atctaccatt attgaaacga cttgagccac
     6061 acatcgacca ttttgtattt gatgccaatt caaatgacgc tgttgatttt aataaaatga
     6121 atgatgatga atttaaaacc gcgagtcaaa tgattattaa taaaacgaat taccttatcg
     6181 acttaatgca tcgtcatcgc ctaaagcgtc cactcatttt actcaattgg aatacattga
     6241 cgggtgatac atttattaca aacggcgaat attttagagg tggtatcatc attgagcaat
     6301 tattaaaatt aagttctaaa gtagagggta tcggttattg gttgaattat gatttgcacg
     6361 tcagtcattg taaaaatgaa cgggattata tgaattctat tgaactattt catcaatata
     6421 atggaaaacg tccggtctat ttcacggcat tgctatttaa taaattaaca agcaatattt
     6481 tgtattctga tgatacatgt attgtcacgg ggactgattc aaattttcaa atattgttat
     6541 atgatgcaaa gcattttaat ccgtacttag cgttggacaa tcaaatgaat atgcgtgcga
     6601 cggaaatgat tcatttgaac attaatgccc tggaagaagg tatgtataag attaaacatt
     6661 ttaccttaga taaagaaaat ggtgcgttat ttaatctttg gcgcaaacat catacgatac
     6721 atggcatgga caaggactct atagattacg ttaatcgaat gagttttccg aaattagaag
     6781 tatatgatat agatatcacg gacacactgg cattaaacat taaaatgatt acgaatggga
     6841 ttcacttaat tgaagtaaaa cgttacccaa gttcataaaa tgatcacaaa tcacaaattt
     6901 tgatatacat aatttgtgat tttttatatt caaagctaaa attgcaaaaa attaatggtt
     6961 aacatctctg ttgttggcaa tataaataat gattaatcat ttatgatgta actaaggaga
     7021 tgaaggatat gaatcaacaa ttaattgaaa ctttaaaatc taaagaaggc aaaatgattg
     7081 agatcagacg ttatttacat cagcatccag aattatcttt tcatgaagat gaaacggcga
     7141 aatacatcgc tgaattttac aaaggtaaag atgtggaagt agaaacgaat gtcggaccac
     7201 gtggaattaa agtaacgatt gattcaggga aacctggtaa aacattagca atccgtgcag
     7261 actttgacgc attacccatt actgaagata caggattatc ttttgcatca caaaataaag
     7321 gtgttatgca cgcatgtggt cacgatgcac atacagcata catgcttgta ttagcagaga
     7381 cgcttgctga aatgaaagat agttttacag gaaaagtcgt tgtgatacat caaccagctg
     7441 aagaagtacc accaggtggt gctaaaacaa tgattgaaaa tggtgtatta gacggtgttg
     7501 atcatgtatt aggtgtacac gtcatgagca caatgaaaac aggtaaagtg tattacagac
     7561 ctggttatgt tcaaacagga cgcgcattct tcaaattgaa agttcaaggt aaaggtggtc
     7621 atggttcatc accacatatg gccaatgatg ccattgttgc aggtagctac ttcgtcacag
     7681 cgttacaaac agttgtatct agacgactaa gtccatttga aaccggtgtt gtcacaatcg
     7741 gttcatttga cggtaaaggt caattcaatg tcattaaaga tgttgttgaa attgaaggtg
     7801 atgtacgtgg attaacagat gctacaaaag caacaattga aaaagaaatt aaacgtttat
     7861 caaaaggatt agaggatatg tatggtgtaa cttgcacctt agaatataac gatgattatc
     7921 cagcattata taatgatcca gagtttactg agtacgtggc taagacgttg aaagaagcaa
     7981 accttgattt tggtgttgaa atgtgtgaac cacaaccacc ttcagaagac tttgcatatt
     8041 atgctaaaga acgcccaagt gcctttattt atacaggtgc agcggtggaa aatggtgaaa
     8101 tttacccaca tcatcatcct aaatttaaca tttcagaaaa atcattactt atttctgcag
     8161 aagcagtagg gacagttgtt ttagattacc ttaaaggaga taactaacat gaatgaaacg
     8221 tatcgcgggg gcaacaagtt aatcttaggt attgtattag gtgttattac attttggttg
     8281 tttgcacaat cacttgtaaa tgttgtaccg aatttacaac aaagttttgg tacagacatg
     8341 gggacaatta gtattgcggt cagtctaact gcactatttt caggcatgtt tgttgttgga
     8401 gcaggtggtc tggcagataa aattgggcgc gtgaaaatga cgaatatcgg tttattatta
     8461 agtattattg gttcagcatt aattattatt acgaatttac cggcattatt aattttaggt
     8521 cgtgttatac aaggtgtatc agcagcgtgt attatgcctt ctacattggc cattatgaaa
     8581 acttattatc agggtgctga acgtcagcgt gccttaagtt attggtctat cggttcttgg
     8641 ggtggcagtg gtatctgttc actcttcggt ggtgcagttg cgacaactat gggttggaga
     8701 tggattttca tcttctcaat tatcgttgcc gtactttcaa tgttactcat caaagggacg
     8761 cctgaaacga aatcagaaat taccaataca cataaatttg acgttgcagg gctaattgtt
     8821 ctagtagtta tgttgctaag tttaaacgtt gtcattacta aaggtgcagc acttggttac
     8881 acatcattat ggttctttgg tttgattgca atcgtaattg tagcattctt tattttctta
     8941 aatgttgaga aaaaagtaga taatccactt attgatttta aattatttga aaataaacca
     9001 tatacaggtg caacgatttc gaacttctta ttaaacggtg ttgcaggtac attaattgta
     9061 gcgaatacat tcgtgcaaca aggtttaggt tatacagcat tgcaggcagg atacttatca
     9121 attacttatt taatcatggt gttattgatg attcgagttg gtgaaaaatt attacaaaaa
     9181 atgggttcta agcgaccaat gttattaggt acattcattg tggtcattgg tattgcactt
     9241 atttcattag tattcttacc aggcatattt tatgttatca gttgtgtcgt aggatattta
     9301 tgtttcggac taggcttagg tatttatgca acaccttcta cagatacagc tatttcgaat
     9361 gcaccgttag ataaagttgg cgttgcttca ggtatttata aaatggcttc atcacttggt
     9421 ggcgcattcg gtgtcgcaat tagtggtgct gtatatgctg gtgcagttgc tgcaacgagc
     9481 attcatacag gtgcgatgat tgcactttgg gttaacgtat taatgggaat catggcattt
     9541 atcgcaattt tattcgcgat tcctaatgat gataaacgtg tcaaagatgc gaaataataa
     9601 cgatgacacg ccaagtgttt tgataatgat ataaatgaaa gtaaagcaaa ttaaatgagg
     9661 aaatgtatag agcagtgcat atagataaat ctatgattgg attaacacta atataaatat
     9721 aaatgaaaat gaaaaaccat ccacttaaat ttttgataag ttaggtggat ggttatttat
     9781 tgcgttgttc taaaaatttg gacatttata tggaaagaac tatatataat gtgaaggtta
     9841 tggcaattcg cttcaatgaa ggcgacgtgt aaaagtgtat attaaacgtt tcggggtcag
     9901 actccgagaa cagtgtccgt catgcttacc gcatactaat gaagcattat tatatcagtg
     9961 tttatagccg atttaagata ataaattttg ctaaacaaaa aagccaaccc atgaatgttg
    10021 gattggcttt ttacatgcat ctgaatctct aattttaaaa aaatatgaat ataaataaga
    10081 cagtaaaaat taaatttcag ttgttgcaat ttcttcatct gtaggtacat catcgttaag
    10141 gccaacaagt gcttcagaaa catttcgtga atgataaccg atacgttcaa gaacaccaat
    10201 catatcgata tatagtaatc cgccttttgt tgtacattca ccacgattaa ggcgtttaat
    10261 atgacctttg cgtagtttat gttcaatatt aaatgattct ctactacgtt ctacaatttc
    10321 atcttttttc gttttgtcat aaacatctaa catgtcgatg gctttatcaa atgactcagc
    10381 aacatggttg aataatttat ccataccgcg ttgtgcatct tctgtaatgc gaatatcttc
    10441 atcatgttgg cgttttaatt gagcgacata ctcttctgtt agctctgcta cttttaaaat
    10501 agagcgattg acatcaaaca taactgctaa acgctcaacg tctgccttcg taatggcttt
    10561 tgtagaaatt ctaactaaat aatttcgaat gctatcattg attgtttcaa cagcttgatg
    10621 cttttgttca agctttttga tcaatttttt atcgtctttt gtaatttcgc gaatgtcttc
    10681 aaacattgat aagacaatct gacccacatt ttgtaattct ttttgagttt cttgtaatgc
    10741 aacaccaggt gcgtgataaa caagatcttt gtttaagtgc tgaggtttat agtcatcagc
    10801 aatatcttta cctgggacaa gctttgtaac aatccatgct aaacctgcta caaatggtaa
    10861 ttgaatcaaa gtatttgtta tgttgaagat accatgtgat actgcaatcg tcatcgctgg
    10921 ttttaagtgc cataaatctt gtaacaaact aatcaaatga atcacaactg gcaagaaaat
    10981 tgtgaagata attaccccga ttaagttaaa gatgacgtgt acaagcgccg cacgttttgc
    11041 agcgattgag ccggctaaac tagctaagat agctgtaatc gtggtaccaa tgttatcgcc
    11101 tagtaacaca gggattgctg cgtttaagct aattaaatct tgttgataaa attcttgtaa
    11161 aataccaatc gtcgcacttg aactttgaac tagtgctgtt aaccctgcgc cgacaatgac
    11221 agcgagtatt ggatttgtag acatatcaag cattaattgc ttaaatccat ctaatgatgc
    11281 taaaggttta acggcatcac ccataaattc tagaccgaag aatagtgaac cgaaaccgaa
    11341 tagtatgcgg ccaatgttat tgattttaga gcgtttaaag aaaaagatta agaatgcacc
    11401 taatgctaaa attggcattg catattcgcc taaatctata ccgataataa atgcagttac
    11461 cgttgttccg atattagcac ccattatcac tccaatggct tgtttcaatg tcataaatcc
    11521 agctgttacc agtccgattg tgataactgt cgtacctgaa ctactttgta ttaaaatagt
    11581 tacaacgata cctgcaataa cacctaatac tggatttgat gtaaatttgt ttaaaatatc
    11641 tcgtagcctg tctcctgctg atgcttgaag cccgtctccc atgattttta agccgtaaag
    11701 gaaaatacct aaaccaccta aaaaggagaa aatgacttct gtaaccgaca tttccattat
    11761 ttcacctcaa ataagcttta tatttagatt atcgcttata attgtaaatt ta
//
LOCUS       DALAGF010000082        11741 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.082, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000082 DALAGF010000000
VERSION     DALAGF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11741
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.082"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            319..3024
                     /gene="acnA"
                     /locus_tag="O2692_002048"
     CDS             319..3024
                     /gene="acnA"
                     /locus_tag="O2692_002048"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCZ4299669.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            3204..3671
                     /locus_tag="O2692_002049"
     CDS             3204..3671
                     /locus_tag="O2692_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCZ4299670.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDGIDSL"
     gene            complement(3878..4174)
                     /locus_tag="O2692_002050"
     CDS             complement(3878..4174)
                     /locus_tag="O2692_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HCZ4299671.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4549..5157)
                     /gene="plsY"
                     /locus_tag="O2692_002051"
     CDS             complement(4549..5157)
                     /gene="plsY"
                     /locus_tag="O2692_002051"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCZ4299672.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLQVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5363..7354
                     /gene="parE"
                     /locus_tag="O2692_002052"
     CDS             5363..7354
                     /gene="parE"
                     /locus_tag="O2692_002052"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCZ4299673.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7354..9756
                     /gene="parC"
                     /locus_tag="O2692_002053"
     CDS             7354..9756
                     /gene="parC"
                     /locus_tag="O2692_002053"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCZ4299674.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDSSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRNGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            10006..11466
                     /locus_tag="O2692_002054"
     CDS             10006..11466
                     /locus_tag="O2692_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCZ4299675.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
BASE COUNT         4100 a         1688 c         2233 g         3720 t
ORIGIN      
        1 atttctgtcc cactcccatc aaaagaatga cattgaaaaa aatattaaag tttggtgcca
       61 taattttctt tctgctcaat gaaataatac gtttaaaaat tgaattgaat cattcgcgaa
      121 attgataatt attctcaatt aataaaaaat acttattaca ccttacctgt atgataagtt
      181 ttgcttatat actctgatta aaaagtcaaa acctaaatta agcgttttct aagcatgatg
      241 agttgtttat caataaatat aataatacaa tttaatcaag gcatataaat ataaaaatgt
      301 atcaaggggg atcattaaat ggctgcaaat tttaaagagc aatcaaaaaa acattttgac
      361 ttgaatggcc aaagttatac ttactatgat ttaaaagctg tagaagagca aggtattact
      421 aaagtttcca atttacctta ttcaattcgt gttttgttag aatctttact tcgtcaagaa
      481 gatgattttg taattacaga cgatcatatt aaagctttaa gtcagtttgg aaaagatgga
      541 aatgaaggcg aggtgccatt taaaccttct cgtgttattt tacaagattt cacaggtgta
      601 ccagccgtag ttgatttagc ttctttacgt aaagcaatgg atgacgttgg gggagatatt
      661 actaaaatta atccagaagt accggtggat ttagttattg accactcagt tcaagtggat
      721 agctatgcaa atccagaagc tcttgaacgt aatatgaaat tagaatttga acgtaactat
      781 gaacgttatc agtttttaaa ttgggcaacg aaagcatttg ataattacaa tgcagttcct
      841 cctgcaactg gaatagttca ccaagttaac ttagaatatt tagcaagtgt tgtacatgtt
      901 cgtgatgtag atggtgaaaa aactgcattt ccagatacat tagttggtac tgattcacat
      961 acaacaatga taaatggtat tggcgtacta ggatggggtg ttggtggtat tgaagctgaa
     1021 gctggaatgc ttggacaacc ttcttatttc ccaattccag aggttattgg tgtacgacta
     1081 gtaaattcat taccacaagg cgcaacagca actgatttag cgttaagagt aactcaagag
     1141 ctacgtaaaa aaggtgttgt tggtaaattt gtggagttct ttggtccagg tgtacaacat
     1201 ttaccactag cagaccgtgc tacaattgca aacatggcac cagagtatgg agcaacttgc
     1261 ggattcttcc cagttgatga tgaatctctt aaatatatga agttaactgg tagatcagac
     1321 gaacatatcg cgctagtaaa agaatatttg aaacaaaacc atatgttctt tgatgttgag
     1381 aaagaagatc ctaattatac agatgttatc gaattggatt tatcaacagt tgaagcatcg
     1441 ctttcaggac caaaacgtcc tcaagattta attttcttaa gtgatatgaa atcatcattt
     1501 gaaaattctg taacagctcc agcaggcaac caaggacacg gtttagataa aagtgaattt
     1561 gataagaaag ctgaaattaa ctttaaagat ggatcaaaag ctacaatgaa aacaggtgat
     1621 attgcaatag cagcaattac atcatgtaca aatacatcta acccttatgt aatgttaggt
     1681 gcaggtttag ttgctaaaaa agcagttgaa aaaggcttga aagttcctga atacgttaaa
     1741 acttctctag caccaggatc aaaagttgtt accggatatt taagagatgc tggcttacaa
     1801 ccttatttag atgatttagg cttcaacttg gttggttatg gatgtacaac ttgtatcggt
     1861 aattcaggtc ctttattacc agaaattgaa aaagcgattg ctgatgagga cctattagtg
     1921 acatctgtat tatctggtaa ccgtaacttt gaaggtcgta tccatcctct tgttaaagcc
     1981 aattacctag cttcaccaca gttagttgtt gcttatgcat tagctggaac ggttgatatt
     2041 gatttacaaa atgaacctat tggtaaaggt aatgacggtg aagatgtata tttgaaagat
     2101 atttggccat caattaaaga agtttcagat accgttgata gtgttgtaac acctgaatta
     2161 tttattgaag aatataataa cgtatacaat aacaacgaat tatggaatga gattgatgta
     2221 actgatcaac ctctatatga ctttgatcct aattcaacat acattcaaaa tccatcattc
     2281 ttccaaggat tatctaaaga accgggtacg attgttccat taaatggttt acgtgttatg
     2341 ggtaaattcg gtgattctgt gacaactgac cacatctctc cagcaggtgc aattggtaaa
     2401 gatacgccag ctggtaaata tttacaagat catcaagtgc ctattcgtga atttaattca
     2461 tatggttcaa gacgtggtaa tcacgaagta atggttcgag gtacgtttgc taatatacgt
     2521 attaaaaacc aattagcgcc aggtactgaa ggtggtttta caacttattg gccaacaaat
     2581 gaagtaatgc ctatctttga tgctgcaatg aaatataaag aagatggtac aggtttagtt
     2641 gtattagctg gtaacgatta tggtatgggt tcatctcgtg actgggcagc aaaaggtaca
     2701 aacttattag gtgttaaaac agttattgca caaagttatg aacgtatcca tcgttcaaat
     2761 ttagttatga tgggtgtatt accattagag tttaaaaaag gtgaatcagc tgattctctt
     2821 ggtctagatg gtacagaaga aatttctgtt aatattgatg aaaatgttca accacatgac
     2881 tacgtcaaag ttactgctaa gaagcaagat ggtgatttgg tagaatttga cgctatggtt
     2941 cgttttgact cacttgttga aatggattac tatcgtcacg gtggaatttt acaaatggtt
     3001 ttaagaaata aattagcgca ataaaaaata gatatcacag taaaatttta atcggtattt
     3061 gaggcacttt gatagtgctc aaaaatactt ctataaactt tatgatataa taggtcggtt
     3121 caaaaccttt gtgttcttgg ccggcctctt cttgtatttt atgttagaat gagatataaa
     3181 gttaaactga aagaaggctt aaaatgatat atagtattac agaaatagaa gcgcgttatg
     3241 ctgaaactga taagatgggt gtaatttatc acgggaatta tgcaacttgg tttgaagttg
     3301 cgcggttgga ttatatatcg aagttaggtt ttagttatgc tgatatggaa aaacaaggaa
     3361 tcatttcacc tgtgactgac ctcaatgtca attataaaaa gtctattttt tatccagaaa
     3421 aagttaaagt taaaacttgg gttgaaaaat attcgagatt acgttcagtg tataaatatg
     3481 agatttttaa tgaaaaaggt gaacttgcaa ctacaggttc cacagaattg atttgcatta
     3541 aagaagatac ttttaagcct atacggttgg atcgttattt cccagattgg catgaagctt
     3601 atagtaaagt gcaaacgctc aataatgaag ggaaaacagt agagataatg gatggtattg
     3661 attctttata agttactgta aatgtttgga gaggcaatat taacaattaa tctataaaaa
     3721 atagccaata cgttaacatt aatgtaatta gcgtgaagat aagctaatat atagcaaaaa
     3781 cgaacttgtt catacaacat cagatgtgct aaaccaaagt acaacttccg aggttcagga
     3841 cacatcagaa tgtgtaaaca agttcgtttt attttagtta ttttgtggaa taagaaattt
     3901 catcttcgtg atcaactaca tttacaataa tgtggtcatc ttcaaagtac cacaaatctt
     3961 tttccgctac gacaacattt aaatcgtcat attgttgttc atagccaata tcaacatctt
     4021 cctttggttc aactgtaaaa gcaggactaa atccttgctt gagttggaat tcgccaccat
     4081 atcttacaaa aaacacgagc actttattat tttcaggcaa ctcaagttca tttttaaacc
     4141 aagttactgc tgcatcagta agttctattt gcataacaat gacctcctat atttattaca
     4201 gaatgggagt gggccaactt gtattatttg tggaatttct tatcgaaatt ctctgtgttg
     4261 gagcccaccc caacctgctc attattgtaa gctgaatttc cataagcttc tatgttggga
     4321 cccccgacca gaattgaaaa aacctagtta taagcgcatt ttgttcggtt aactacagct
     4381 aatataacat tgtggagcct atgacattga tttatgtctc ggtctcaaat gttcttgtaa
     4441 aaaaactaag tatagttgaa ttttaagtta aaaaaattgt ttttgcaaaa ataaaataat
     4501 ccatcaactg caaaaaatat gcaatgatgg actaatgtca aaaaattgtt acatccattt
     4561 tattttaggt tcttcgcctc taaaaatcct tgcgatatta gagcgatgtc taattatcaa
     4621 tatgattgaa actaagaaac taacgactaa taaaatatag tcttgaatga taagcgagcc
     4681 aatcacacag caaattgctg caacgatact tgctaaagaa acatatttaa aaatcttcaa
     4741 tacaataaag aagataattg caagtattag taaaagtatc ggattgactc ccaagacgac
     4801 acctgcacta gttgcaacag ctttgccacc ttggaatttt aaataaacag gataaacgtg
     4861 tccaagtata gcgaataagc caacaattaa accatttgta aaaaaagtac taatagggcc
     4921 atctgcgtga acttgtaacc ataaagggaa gaaaacagtt atgaaccctt tgaaaatatc
     4981 tagaaatgtt accaagaatc ctgcaggacg acctaatact ctaaagctat tagtagcgcc
     5041 agtattacca ctaccaaatt gtctaatatc ttttttgaaa aataattttc caattacgaa
     5101 tccacttggg aaagcgccga taagataact tagtagtaac atgacgatta tcatcataaa
     5161 tattacacat cctttaatat cttaggacta ttttatcata ttcatttaaa ttacggctaa
     5221 aaattttaaa aacggggatt aatatatgga attaagctat gaaagttaat tgatacttgc
     5281 attttacgct gatttatata agaataacta ttgtatagtt ttaaaaacga acgtacgttt
     5341 gcaggaggcg aaatcattgg caatgaataa acaaaataat tattcagatg attcaataca
     5401 ggttttagag gggttagaag cagttcgtaa aagacctggt atgtatattg gatcaactga
     5461 taaacgggga ttacatcatc tagtatatga aattgtcgat aactccgtcg atgaagtatt
     5521 gaatggttac ggtaacgaaa tagatgtaac aattaataaa gatggtagta tttctataga
     5581 agataatgga cgtggtatgc caacaggtat acataaatca ggtaaaccga cagtcgaagt
     5641 tatctttact gttttacatg caggaggtaa atttggacaa ggcggctata aaacttcagg
     5701 tggtcttcac ggtgttggtg cttcagttgt aaatgcattg agtgaatggc ttgaagttga
     5761 aatccatcga gatggtaata tatatcatca aagttttaaa aacggtggtt cgccatcttc
     5821 tggtttagtg aaaaaaggta aaactaagaa aacaggtacc aaagtaacat ttaaacctga
     5881 tgacacaatt tttaaagcat ctacatcatt taattttgat gttttaagtg aacgactaca
     5941 agagtctgcg ttcttattga aaaatttaaa aataacgctt aatgatttac gcagtggtaa
     6001 agagcgtcaa gagcattacc attatgaaga aggaatcaaa gagtttgtta gttatgtcaa
     6061 tgaaggaaaa gaagttttgc atgacgtggc tacattttca ggtgaagcaa atggtataga
     6121 ggtagacgta gctttccaat ataatgatca atattcagaa agtattttaa gttttgtaaa
     6181 taatgtacgt actaaagatg gtggtacaca tgaagttggt tttaaaacag caatgacacg
     6241 tgtatttaat gattatgcac gtcgtattaa tgaacttaaa acaaaagata aaaacttaga
     6301 tggtaatgat attcgtgaag gtttaacagc tgttgtgtct gttcgtattc cagaagaatt
     6361 attgcaattt gaaggacaaa cgaaatctaa attgggtact tctgaagcta gaagtgctgt
     6421 tgattcagtt gttgcagaca aattgccatt ctatttagaa gaaaaaggac aattgtctaa
     6481 atcacttgtg aaaaaagcga ttaaagcaca acaagcaagg gaagctgcac gtaaagctcg
     6541 tgaagatgct cgttcaggta agaaaaacaa gcgtaaagac actttgctat ctggtaaatt
     6601 aacacctgca caaagtaaaa acactgaaaa aaatgaattg tatttagtcg aaggtgattc
     6661 tgcgggaggt tcagcaaaac ttggacgaga ccgcaaattc caagcgatat taccattacg
     6721 tggtaaggta attaatacag agaaagcacg tctagaagat atttttaaaa atgaagaaat
     6781 taatacaatt atccacacaa tcggggcagg cgttggtact gactttaaaa ttgaagatag
     6841 taattataat cgtgtaatta ttatgactga tgctgatact gatggtgcgc atattcaagt
     6901 gctattgtta acattcttct tcaaatatat gaaaccgctt gttcaagcag gtcgtgtatt
     6961 tattgcttta cctccacttt ataaattgga aaaaggtaaa ggcaaaacaa agcgagttga
     7021 atacgcttgg acagacgaag agcttaataa attgcaaaaa gaacttggta aaggcttcac
     7081 gttacaacgt tacaaaggtt tgggtgaaat gaaccctgaa caattatggg aaacgacgat
     7141 gaacccagaa acacgaactt taattcgtgt acaagttgaa gatgaagtgc gttcatctaa
     7201 acgtgtaaca acattaatgg gtgacaaagt acaacctaga cgtgaatgga ttgaaaagca
     7261 tgttgagttt ggtatgcaag aggaccaaag tattttagat aattctgaag tacaagtgct
     7321 tgaaaatgat caatttgatg aggaggaaat ctagtgagtg aaataattca agatttatca
     7381 cttgaagatg ttttaggtga tcgctttgga agatatagta aatatattat tcaagagcgt
     7441 gcattgccag atgttcgtga tggtttaaaa ccagtacaac gtcgtatttt atatgcaatg
     7501 tattcaagtg gtaatacaca cgataaaaat ttccgtaaaa gtgcgaaaac agtcggtgat
     7561 gttattggtc aatatcatcc acatggagac tcctcagtgt acgaagcaat ggtccgttta
     7621 agtcaagact ggaagttacg acatgtctta atagaaatgc atggtaataa tggtagtatc
     7681 gataatgatc cgccagcggc aatgcgttac actgaagcta agttaagctt actagctgaa
     7741 gagttattac gtgatattaa taaagagaca gtttctttca ttccaaacta tgatgatacg
     7801 acactcgaac caatggtatt gccatcaaga tttcctaact tactagtgaa tggttctaca
     7861 ggtatatctg caggttacgc gacagatata ccaccacata atttagctga agtgattcaa
     7921 gcaacactta aatatattga taatccggat attacagtca atcaattaat gaaatatatt
     7981 aaaggtcctg attttccaac tggtggtatt attcaaggta ttgatggtat taaaaaagct
     8041 tatgaatcag gtaaaggtag aattatagtt cgttctaaag ttgaagaaga aactttacgc
     8101 aatggacgta aacagttaat tattactgaa attccatatg aagtgaacaa aagtagctta
     8161 gtaaaacgta tcgatgaatt acgtgctgac aaaaaagtcg atggtatcgt tgaagtacgt
     8221 gatgaaactg atagaaatgg tttacgaata gcaattgaat tgaaaaaaga tgtgaacagt
     8281 gaatcaatca aaaattatct ttataaaaac tctgatttac agatttcata taatttcaac
     8341 atggtcgcta ttagtgatgg tcgtccaaaa ttgatgggta ttcgtcaaat tatagatagt
     8401 tatttgaatc accaaattga ggttgttgca aatagaacga agtttgaatt agataatgca
     8461 gaaaaacgta tgcatatcgt tgaaggtttg attaaagcgt tgtcaatttt agataaagta
     8521 atcgaattga ttcgtagctc taaaaacaag cgtgacgcta aagaaaacct tatcgaagta
     8581 tacgagttca cagaagaaca ggctgaagca attgtaatgt tacagttata tcgtttaaca
     8641 aatactgaca tagttgcgct tgaaggtgaa cataaagaac ttgaagcatt aatcaaacaa
     8701 ttacgtcata ttcttgataa ccatgatgca ttattgaatg tcataaaaga agaattgaat
     8761 gaaattaaaa agaaattcaa atctgaacga ctgtctttaa ttgaagcaga aattgaagaa
     8821 attaaaattg acaaagaagt tatggtgcct agtgaagaag ttattttaag tatgacacgt
     8881 catggatata ttaaacgtac ttctattcgt agctttaatg ctagcggtgt tgaagatatt
     8941 ggtttaaaag atggtgacag tttacttaaa catcaagaag taaatacgca agataccgta
     9001 ctagtattta caaataaagg tcgttatcta tttataccgg ttcataaatt agcagatatt
     9061 cgttggaaag aattgggaca acatgtatca caaatagttc ctatcgaaga agatgaagtg
     9121 gttattaatg tctttaatga aaaggacttt aatacagatg cattttatgt ttttgcgact
     9181 caaaatggca tgattaagaa aagtacagtg cctctattta aaacaacgcg ttttaataaa
     9241 cctttaattg ctactaaagt taaagaaaat gatgatttga ttagtgttat gcgctttgaa
     9301 aaagatcaat taattaccgt cattactaat aaaggtatgt cattaacgta taatacaagt
     9361 gaactatcag ataccggatt aagggcagct ggtgttaaat caataaatct taaagctgaa
     9421 gatttcgttg ttatgacaga aggtgtttct gaaaatgata ctatattgat ggccacacaa
     9481 cgcggctcgt taaaacgtat tagttttaaa atcttacaag ttgctaaaag agcacaacgt
     9541 ggaataactt tattaaaaga attaaagaaa aatccacatc gtattgtagc tgcacatgta
     9601 gtgacaggtg aacatagtca atatacatta tattcaaaat caaatgaaga acatggttta
     9661 attaatgata ttcataaatc tgaacaatat acaaatggct cattcattgt agatacagat
     9721 gattttggtg aagtaataga catgtatatt agctaaaaac tatatgcaat cacgaaatta
     9781 aatgataaaa tacagtaatg ttaaattttg actaaattca agggatttat attaaatgct
     9841 gaccaagtac ttatcgttaa attagcgata tactttgacc aaactaatat aaatctcttg
     9901 tattgtataa gaaatgatga tgtatctcta tatttactga aggttcaatt cggtctaatt
     9961 ttatagaagg tacatgattc atgtagagga tgcgaggggt ttattttgaa agatttcgat
    10021 agtttaatac ctggatggtt taaagaattt gtccatgttg gtaccgattt aatatggtct
    10081 caatatttaa ttggtctatt attgacagct ggattcttct ttacgattag ttctaaattc
    10141 gttcaattac gaatgttacc tgaaatgttt agagctttag tagaacgtcc agaaacttta
    10201 gaagatggta agaagggtat ttcgccattc caagcatttg cgattagtgc tggttcgaga
    10261 gttggtactg gtaatattgc tggtgttgcg actgcgattg ttttaggcgg tccaggtgca
    10321 gtattttgga tgtgggttat tgcatttata ggtgcagcga gtgcatttat agaagcgact
    10381 ttggctcagg tttataaagt acatgataaa gatggtggat tccgtggtgg tccagcttac
    10441 tatattacta aaggtttaaa tcaaaaatgg ctaggtatcg tatttgcgat tttaattaca
    10501 attacatttg catttgtatt taacacagtg caatctaata caattgcgga gtcgttaaat
    10561 acgcaatata atattagtcc agtaatcaca ggtattattt tagcaatcgt aacagctatt
    10621 attatatttg gtggtgtacg tagtattgct acgttatctt cgttaattgt accgattatg
    10681 gctatcattt acattggtat ggttttagta atattgctat ttaatttaga tcaaattgtt
    10741 cctatgatag gtacgattat taaaagtgca tttggtatcg aacaagtaac tggtggcgct
    10801 gtaggtgctg cggttcttca aggtatcaaa cgtggtttat tctctaacga agctggtatg
    10861 ggttctgcgc cgaatgcagc ggcaactgct gccgtaccac accctgttaa gcaaggttta
    10921 atccaatcat taggtgtgtt ctttgataca atgttggttt gtacagcaac tgcaatcatg
    10981 attttactat attcaggact gaaatttggt gataacgcac ctcaaggtgt tgcagttact
    11041 caatcagcac ttaatgagca tttaggttct gctggaggta ttttcttaac aatagcagtt
    11101 acactgtttg cattttcatc tgttgtaggt aattactatt acggtcaatc taatattgaa
    11161 tttttatcaa caaaccgtgt aatattattt atctttagat gtcttgttgt agtacttgtc
    11221 tttgtcggtg cagttgtaaa aacagaaaca gtatggaata cggcagactt atttatgggc
    11281 ttaatggcaa ttgtaaacat tatttccatt ataggactgt ccaatgtagc ttttgcattg
    11341 atgaaagatt atcaaaagca gaaaaaagaa ggcaagaacc ctgtctttaa acctgaaaac
    11401 ttagaaatta acttatttgg aattagtgct tggggcgcta acaaatataa gaactctgat
    11461 aaataaatat ttttaatgta acgattcatt atataaaaat caaataaacc ctgaggtttt
    11521 caaattaaag cgaaaatctc agggttttaa atttgtactc aattatcatc tgtatcatta
    11581 cactagttaa tctaagattt gattatatat taatatagta aggttgagat ttatttcctc
    11641 cattggtgtg caaagactat accattgata agacatattg ttggatagat gaatataaat
    11701 ttgttttagc tttgcacaat ttaatagtac gttttagttt g
//
LOCUS       DALAGF010000083        11641 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.083, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000083 DALAGF010000000
VERSION     DALAGF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11641
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.083"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            56..1456
                     /locus_tag="O2692_002055"
     CDS             56..1456
                     /locus_tag="O2692_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4299676.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
     gene            2000..3376
                     /locus_tag="O2692_002056"
     CDS             2000..3376
                     /locus_tag="O2692_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCZ4299677.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            3378..4517
                     /locus_tag="O2692_002057"
     CDS             3378..4517
                     /locus_tag="O2692_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCZ4299678.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            4492..4857
                     /locus_tag="O2692_002058"
     CDS             4492..4857
                     /locus_tag="O2692_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HCZ4299679.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            4860..5129
                     /locus_tag="O2692_002059"
     CDS             4860..5129
                     /locus_tag="O2692_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299680.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(5330..5497)
                     /locus_tag="O2692_002060"
     CDS             complement(5330..5497)
                     /locus_tag="O2692_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HCZ4299681.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(6004..6834)
                     /gene="thiD"
                     /locus_tag="O2692_002061"
     CDS             complement(6004..6834)
                     /gene="thiD"
                     /locus_tag="O2692_002061"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCZ4299682.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            7018..7674
                     /locus_tag="O2692_002062"
     CDS             7018..7674
                     /locus_tag="O2692_002062"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCZ4299683.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            7675..8055
                     /locus_tag="O2692_002063"
     CDS             7675..8055
                     /locus_tag="O2692_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HCZ4299684.1"
                     /translation="MLNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTVAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            8188..8556
                     /locus_tag="O2692_002064"
     CDS             8188..8556
                     /locus_tag="O2692_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCZ4299685.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            8677..10161
                     /locus_tag="O2692_002065"
     CDS             8677..10161
                     /locus_tag="O2692_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCZ4299686.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(10303..10755)
                     /locus_tag="O2692_002066"
     CDS             complement(10303..10755)
                     /locus_tag="O2692_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCZ4299687.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(10768..11532)
                     /locus_tag="O2692_002067"
     CDS             complement(10768..11532)
                     /locus_tag="O2692_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCZ4299688.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
BASE COUNT         3926 a         1805 c         2127 g         3783 t
ORIGIN      
        1 aaaactaatt aactccgtat aattatgaaa catacaagag ggagtgtatg aattcatgga
       61 ttttaataaa gagaatatta acatggtgga tgcaaagaaa gctaaaaaaa ccgttgttgc
      121 aaccggtatc ggtaatgcaa tggaatggtt cgattttggt gtctatgcat atacaactgc
      181 gtacattgga gcgaacttct tctctccagt agagaatgca gacattcgac aaatgttgac
      241 tttcgcagca ttagccattg cgtttttatt aagaccaatt ggtggtgtcg tatttggtat
      301 tattggtgac aaatatggac gtaaagttgt attaacatct acaattattt taatggcatt
      361 ttcaacatta accattggat tattgccaag ctatgatcaa attggacttt gggcaccaat
      421 actattattg cttgcaagag tactacaagg gttttcaaca ggtggagagt atgcgggggc
      481 aatgacatat gttgccgaat catctccaga taagcgtcgt aactcattag gtagtggact
      541 agaaattggg acattatcag gttacatagc tgcttcaatt atgattgctg tattaacatt
      601 ctttttaaca gatgaacaaa tggcatcatt tggttggaga atcccattct tactcggttt
      661 attcctagga ttattcggct tatatttacg tcgtaagctg gaagaatcac cagttttcga
      721 aaatgatgtt gcaacacaac cagaaagaga taacattaac tttttacaaa tcatcagatt
      781 ttattacaaa gatatatttg tatgttttgt agctgttgta ttcttcaatg ttacaaacta
      841 tatggtaact gcatatttac caacctattt agaacaagtt attaaattag atgcaacgac
      901 aacaagtgta ttaattactt gtgtcatggc aataatgatt ccattagcat taatgtttgg
      961 taagttagcg gataaaatag gtgaaaagaa agtatttcta attggtactg gtgggctaac
     1021 attattcagt atcatcgcat ttatgttatt acattcacaa tcatttgttg taatagtaat
     1081 cggtatattt atattaggat ttttcttatc aacttacgaa gcgacaatgc cagggtcgtt
     1141 accaacgatg ttttacagtc atataagata tcgaacttta tcagtaacat ttaatatctc
     1201 tgtttcgata tttggtggta cgacgccatt agttgcaaca tggttagtta cgaaaactgg
     1261 agatccatta gcacctgcgt attatttaac agcaatcagt gttattggct ttttagttat
     1321 tacattctta catttaagta cagcaggaaa atctctaaaa ggttcgtatc caaatgtaga
     1381 taacgagcaa gatagagctt attatgcaga acatccaaaa gaagcattat ggtgggttaa
     1441 agaacgtaag aattagagat tttaataaaa agtataaatc aatcgtatat aagcacttta
     1501 aagctagtag gttctgctaa ctttaaagtg ctttttaaat tgagaactgt aattagccgt
     1561 aataaagttt ttgtatatac ataaaccccc actgcaatga ttatcgcaat gggggaaaga
     1621 ggggacttaa agcatatgtt tagctttgaa tacttaaaat tctcttgcta ttgaaatgtt
     1681 aggatgtaaa tatgtcttag agtattttgt ccaacgcaat taatattgag actctaacct
     1741 tcaatattat tatagagaac acaaacttaa atagattggg tgacttattt gtgtcagtta
     1801 ttgcgattgc gataacttct tttctctata tacatatagt aacgtcttat ctaataaaaa
     1861 acatggtact acagtatcaa atttatctag ggcttaagtt tgatttttat aataggcagg
     1921 tttacctgat aaaaatactt attcattata taatgttaac aatatgtatt ttaaagttta
     1981 cattgagtga gggatattga tgaacgtaat tttagaacag ttgaaaacac atactcaaaa
     2041 taaacctaat gacatagcat tacatatcga tgatgaaaca attacatata gtcaactaaa
     2101 tgcccgcatc actagcgcag ttgaatcttt gcagaaatat tcacttaacc ctgtcgttgc
     2161 tattaatatg aaatcaccgg tgcaaagtat tatttgttat ttagctttgc atcgtttaca
     2221 taaagtgcct atgatgatgg aaggtaaatg gcaaagtact atacatcgtc aattgattga
     2281 aaaatatggt attaaagatg taattggaga tacaggtctc atgcagaata tagactcacc
     2341 gatgtttatt gattcaacgc aattacagca ctaccccaat ttattacata ttggttttac
     2401 ttcagggaca actggactgc caaaagcata ttatcgtgat gaagattcat ggttggcttc
     2461 ttttgaagtt aatgaaatgt tgatgttaaa aaatgaaaat gcaatagcag cccctggacc
     2521 actatcgcac tcgttaacat tatatgcgtt attgtttgct ttaagttccg gtcgtacttt
     2581 tataggacag accacttttc atcctgaaaa gttacttaat caatgtcata aaatatcatc
     2641 atacaaagtt gctatgtttc ttgttccaac gatgattaaa tcattattgt tagtttacaa
     2701 caatgaacat acaatccaat cattttttag cagtggagat aagctgcatt cttctatttt
     2761 taaaaagata aaaaatcaag caaatgacat aaatttgatt gaattttttg gtacatcgga
     2821 aaccagtttt atcagctata acttgaatca gcaagcacca gttgaatcag taggtgtgct
     2881 atttccaaat gtggaattga aaacaacgaa tcacgatcac aatggtatag gaactatttg
     2941 tataaaaagt aatatgatgt ttagtggcta tgtaagtgaa caatgtataa ataatgatga
     3001 atggtttgtt actaatgata atggctatgt aaaagagcag tatttatatt taacgggacg
     3061 tcaacaggat atgttaatta ttggtggtca aaatatatat ccagcacatg ttgaacgcct
     3121 tttaacgcaa tcttcgagca ttgatgaagc aattatcatc ggtattccaa atgagcgttt
     3181 tggtcaaata ggcgtattgc tttattctgg tgatgtgaca cttacacata aaaatgtaaa
     3241 acaattttta aaaaagaaag tgaaacgcta tgaaattcca tcgatgattc atcatgtaga
     3301 aaagatgtat tacactgcaa gtggtaaaat tgctagagaa aaaatgatgt cgatgtattt
     3361 gagaggtgaa ttataatatg aatcaagcag tcatagttgc agctaaacga actgcatttg
     3421 ggaaatatgg tggcacttta aaacatttag agccagaaca attgcttaaa cctttattcc
     3481 aacattttaa agagaagtat ccagaggtaa tatctaaaat agatgatgta gttttaggta
     3541 atgttgttgg gaatggtggc aatattgcaa gaaaagcatt gcttgaagcg gggcttaaag
     3601 attcaatacc tggcgtcaca atcgatcggc aatgtgggtc tggacttgaa agtgttcaat
     3661 atgcatgtcg catgatccaa gccggagctg gcaaggtata tattgcaggt ggtgttgaaa
     3721 gtacaagtcg agcaccttgg aaaatcaaac gaccgcattc tgtgtacgaa acagcattac
     3781 ctgagtttta tgagcgtgca tcatttgcac ctgaaatgag cgacccatca atgattcaag
     3841 gtgctgaaaa tgtggccaag atgtatgatg tttcaagaga attacaagat gaatttgctt
     3901 atcgaagtca tcaattgaca gcggaaaatg taaaaaatgg aaatatttct caggaaatat
     3961 tacctataac cgttaaagga gaaatattca acactgatga aagtctaaaa tcacatattc
     4021 cgaaagataa ctttggccga tttaagcccg tgatcaaagg tgggaccgtt accgctgcga
     4081 atagttgtat gaaaaatgat ggtgcagttt tattgcttat tatggaaaaa gatatggcat
     4141 acgaattagg tttcgagcat ggtttattat ttaaagatgg tgttacggta ggtgttgatt
     4201 ctaattttcc tggcattggt ccagtaccag ccatttccaa cttactaaaa agaaatcaat
     4261 taacgataga aaatattgaa gtcattgaaa ttaacgaagc gttcagtgca caggtagttg
     4321 cctgccaaca agctttaaat atttcaaata cgcaattaaa tatatggggt ggtgcattag
     4381 catcaggtca tccatacggt gcaagcggtg cccaattagt gactcgatta ttttatatgt
     4441 ttgacaaaga gactatgatt gcatctatgg ggataggggg aggtctagga aatgcagcat
     4501 tatttactcg attctaacca gcgattaaat gtgtcatttt ctaaggatag tgtggctgca
     4561 tattatcagt gttttaacca accttataga aaagaagtac caccattaat gtgtgcgtca
     4621 ttatggccaa aatttgattt atttaaaaaa tatgcaaata gcgaactgat tttaacaaaa
     4681 tcagcaatta atcaaactca aaagatagaa gtagacacaa tatatgtagg gcatttagaa
     4741 gatattgaat gccgacagac tcgcaatatc acacgttata caatggcttt aacattaact
     4801 aaaaatgatc aacatgtcat aacggttaca caaactttta ttaaggcgat gaagtagaga
     4861 tgaagtttaa tgagatatgg ataaatgaat atttggcgct cgtaaatgat gataatccaa
     4921 tacataatga gattgtgcca ggacaattag tgagtcaaat gatgctgatg gctatgtcat
     4981 tagagacaaa ccagtgtcaa attaactacg ttaaacctat tttaataaat gaaaatatcg
     5041 aattcattga acaacacgaa cacgaaatta tagcaattaa tgacgatgga gagattaaaa
     5101 taaaaatttc tttgagcaca aaaaaataac cgatattagc tgcatgaacg catattaatt
     5161 aggagatgaa aggacagcta atatcagtta tgtattgtta ttattattgg gaacagagat
     5221 gaatataggt tacgtttctt tctttgcacg gggatgcatt aatctaaaat aataataaca
     5281 actatatcaa tgtttaataa attctggatt attggaacga ttagtcaatt taactaactt
     5341 tcatatgatc tatatcgtct tgtaataaag agagcaattt gaatatttca gtatcactaa
     5401 atgaatcgtc acatttaatt gaaacatgct gaaacgtttt ggttataatt tcataaactg
     5461 gtgcgccttc atggtgatac tgtcgataaa taatcataac ctatattacc tcctttgcta
     5521 ctctatggtt atattataaa taacattttt atgtgtgaca tcaaccttaa gtatcaactt
     5581 tttatcagac atagaacgta tgatttacta agactattta tgtataaaag ttctaaataa
     5641 atatatattt atagagtcgc ctggcagtca tttgggaaat ataacatata tgattagaga
     5701 ggcatctatc gcaaaagaat gataatgata gaggtattga gcatatagat gagtttaagt
     5761 tcatcttgaa aataaagggt tatttagtca tagatgtaga tgtataggaa atatttgtat
     5821 gtattgttcg atatgtatga aattttcaat aaaagctaat aacgcttata tgtaactttc
     5881 aaatttaaat tatatacaga gcatgatgat tataaaaaaa taaccacatc acataaattg
     5941 agttcatacc caatttaagt ggtgtggcta ataatgttga tttatagatg aaccgcctaa
     6001 tcgttaaacc tctgttactt caacatcgat atgttcaata cggttgtatg caccgtgatc
     6061 cacaggacca acaaaatcat tcattttcca accgttttta atagcagaag cgacgaaagc
     6121 tttcgcgcta atcacagctt ctttcggtga cttaccgtta gctaaatatg cagttgttgc
     6181 cgcagcaaat gtacaaccag caccatggtt ataactttgt tggaacatgt ctgttgttag
     6241 ttgataaaat gtttgaccat catagtataa gtcatacgat ttatcttgat ctaaagcttt
     6301 gccaccttta atgatgacat gctgtgcgcc tttatcaaag ataattgttg cagccttttt
     6361 catatcttca attgaattta atttacctaa tcctgataat tgacccgctt caaataagtt
     6421 tggtgtcact accgttgctt taggtagtaa atatttaatc atcgcctcag tatttccagg
     6481 attaagcact tcatcttcgc ctttacaaac catgacagga tctactacaa aatattgtgc
     6541 attagatgcc tcatatactt ctccagcacg tttgattatc tcctcagtac ctaacatacc
     6601 tgttttaata gcatcaggtc cgattgataa agccgtttca agttgttttt caaatacatc
     6661 cattggtaat ggtgtaacat cgtgtgacca tgtatcttta tccatagtaa cgatggcagt
     6721 taaagcgacc atgccatacg tatctaattc ttggaacgtt ttcaaatctg cttgcatacc
     6781 tgcgccagca cttgtgtcag aaccggcaat tgttaaaact ttctttaaag ccattgagct
     6841 tcactcctac ataataatat tgtattcatc atatcatttt taacctaatt gaaaaatatt
     6901 aagcattcaa tatttgatga ttgttgaaat gaatcattca tactattgta acttttgaaa
     6961 atgtcattca ctttagataa gtgtgatatg ttaaaatatg tcctgaggtg agattgaatg
     7021 gaatggtcgc aaatttttca tgacataaca acgaaacatg actttaaagc tatgcatgat
     7081 tttttagaaa aagaatattc gactgcaatc gtataccctg atagggaaaa tatatatcaa
     7141 gcgtttgatt taacaccgtt tgaaaatatc aaagttgtta tattaggaca agacccgtat
     7201 catggtccaa accaagcaca tggattagca ttttcagtgc aacctaacgc aaaattccct
     7261 ccatctttac gtaatatgta taaagaatta gcagatgata ttggatgcgt tagacaaaca
     7321 ccgcatttac aagattgggc aagagaaggc gtcttgttat tgaatacagt tttaaccgta
     7381 agacagggtg aagcaaattc tcatcgtgat attggttggg aaacatttac tgatgaaatt
     7441 attaaagcag tgtctgatta taaagaacat gttgtcttta ttttgtgggg gaaacctgca
     7501 cagcaaaaaa taaagcttat cgatacatct aaacattgta ttataaaatc agtgcatcct
     7561 agtccactgt ctgcatatag aggattcttt ggatcaaaac cgtattccaa agcgaatgcc
     7621 tatttagagt cagtaggaaa atcaccaatt aattggtgtg aaagtgaggc gtagatgttg
     7681 aatagagaaa ctttaatagc acgaattgag caagaattag tacaagcaga gcaggcacag
     7741 catgaccatg actttgaaaa acatatgtat gccatacata tattaacatc tttatatgct
     7801 tcaacatcaa atacaccaca tattggtgaa caacaaatga atcgtcgtat tgctaaccat
     7861 aatcaaatgc cacaatcaca aataacgcag ccaactcatc aagtgacagt tgctgaaatt
     7921 gaagcgatgg gtggtaaagt aaatacgcat tcagcacatc atcataataa gtcatattca
     7981 caaccttcaa accaacaaca aagattagcg acagatgatg acattggcaa tggtgaatcc
     8041 atatttgatt tttaaaaagc aacaatgaaa cataattact taatagcttg ttaagtatgt
     8101 aggttaataa tcaagacgca tatactttta ttcgagtgtt cggatttaaa catttattaa
     8161 tactgaatta tataaggaga ggtagcaatg aaattattta ttattttagg tgcattaaac
     8221 gcgatgatgg ctgtcggtac aggtgcattt ggtgcgcatg gtttacaagg aaaaataagt
     8281 gatcactatt tatcagtatg ggaaaaagca acgacgtatc aaatgtacca tggcttagca
     8341 ttattaatta taggtgtaat tagtggtaca acttcaatca atgttaactg ggctggctgg
     8401 ttaatatttg ctggtattat tttctttagt ggatcattat atattttagt attaactcaa
     8461 attaaagttt taggtgcgat tacgccaatt ggtggcgtat tgttcatcat tggatggata
     8521 atgttaatca ttgcgacatt caaatttgct ggttaaattt taaaacttta gattacctat
     8581 gtaactaaac attaaatttt taataaaaat aatcaagaaa aagagttaca aactcatctt
     8641 ttgggtatag aataccttcg aggtgagttt ttatttatgg aaaaaaagaa taagcaaata
     8701 gatagaggcg atttaaaaca aaacctatct gaaaagtttg tatgggcgat tgcatatggt
     8761 tcatgtatcg gatggggcgc attcatctta ccaggagact ggattaagca gtcaggtccg
     8821 attgcagcat caattggtat agttattggt gcattattaa tgatattaat tgcggttagt
     8881 tatggcgcat tagtagagag atttccagta tcagggggcg cgtttgcctt tagtttctta
     8941 agtttcggca gatatgtgag tttcttctca tcatggtttt taacttttgg ttatgtctgt
     9001 gtcgttgctt taaatgcgac cgcattcagt ttactagtta aattcttatt gccagatgtc
     9061 ttaaataatg ggaaactata caccattgcg ggctgggacg tttatattac ggaaatcatt
     9121 attgcgaccg tattactact tgtattcatg ctagtaacga ttcgtggcgc aagtgtatct
     9181 ggatcattac aatattattt ctgtgtggcg atggtaatcg tcgtattatt gatgttcttt
     9241 ggttcattct ttggtaataa ttttgcactt gaaaatttac aaccgttagc tgaacctagc
     9301 aaaggatggt tagtgtctat tgtggttatt gtatccgtgg caccatgggc atatgttgga
     9361 tttgataata ttccacaaac agcagaagag tttaactttg caccaaacaa gacatttaag
     9421 cttatcgtgt acagtttatt agcagcatca ttaacttatg ttgtcatgat tttatacact
     9481 ggttggttat caacaagtca tcaaagttta aatgggcagt tgtggttaac aggtgctgtt
     9541 acacaaacag catttggtta tattggatta ggtgtattag caattgcaat tatgatgggt
     9601 atatttactg gtttaaatgg attcttgatg agttcaagtc gcttgttatt ttctatggga
     9661 cgttcaggta ttatgccaac aatgtttagt aaattacata gtaaatacaa aacaccatat
     9721 gtcgcaatca tattcctagt aggagtgtcg ttaattgcac cttggctagg aagaactgca
     9781 ttgacttgga ttgtagatat gtcatctact ggtgtatcca ttgcctactt tattacatgt
     9841 ttgtctgcag cgaaattatt cagttataac aaacaaagta atacgtatgc accggtttac
     9901 aaaacgtttg ctattatcgg ctcatttgta tcattcattt tcttagcgtt gttattagtg
     9961 ccaggttctc ctgcagcact gactgcaccg tcttatattg cattacttgg atggttaatc
    10021 atcggtttaa tattctttgt gattcgatat cctaaattga aaaatatgga taatgatgaa
    10081 ttaagtcgct tgattttaaa tagaagtgaa aatgaagttg atgatatgat tgaagaacct
    10141 gaaaaagaaa aaactaaata ataaaagaat cgcacaataa accttcttca ttcggaggcg
    10201 tatcgtgcga ttttttgtat tataaattga catttaagac gaggcagctg aaccttatat
    10261 ataattgcta agagttaggg ctgagccatt tctaacaaat atttataatc gtttaaaaga
    10321 tttcacgaac ccagaaacaa ttaatttgga aatttggtcg gcgaataata aacctaatgc
    10381 gatggcgcct gcaataagtg taacctctag catggtattg attgctgtac tgaaatttaa
    10441 taagactaaa ttttttgtag catcgtatgc taagccacca ggtactaatg gaatgatacc
    10501 cgttaccata aaaatgatgg caggttcttt ttgtttacga gccatataat gacttaacaa
    10561 gcctaatgct aaactaccaa agaaactaga gtatatagtg tgcacattaa agccgttgaa
    10621 gaataaggtg taaaccatcc atccacacgt accaacgaaa ccacatgata gatataattt
    10681 tctaggtgca tcaaaaatga cgcagaagaa cattgaagct aaaaagctaa agataaagtt
    10741 taagatccaa aacatagtct gatactccta tactaaaatt aatacgctac caacgccagc
    10801 accgatgcca aacgcagtaa ccaatgcttc taatgatttc gttgtgaaca tcaacatgtg
    10861 tccaccaaat aaatcttgta ttgcgtttgt tattaataca ccaggaacaa taggcatgac
    10921 tgccgcaatg ataatagttg ccaagtcacc tgttggaata agtgtatgtc caataacggc
    10981 gataatccca ataactaatg aaccaatgaa ttctgggata aactgtgcgt gtaacttacg
    11041 atctaaaatc tcagtgacta ggtatcctag actacctgct aatatcgcag ttaaaacatc
    11101 aatcaatcta ccaccttgta aatataagaa actcattgca atcattgctg cagcaaaacc
    11161 tttaaaggga agactgctgt cacgcttagc aacatatatt ttttcaagtt gcgtttttgc
    11221 ttcggctaaa gaaatttcat tgtttgtaat ttgacgcgaa attttattag cttgagaaat
    11281 ttttattaag tttgtatctc gagaggtaat tctaaatatt ctaggaaacg attccgaatg
    11341 taacgtaaac tggatgacag tgtttgtaac aaagctgtta ctttcactgt aaccaagttt
    11401 ttttgcaata cgtgtcatgg tatcttctac acgcgtacct tctgcaccag attctaatag
    11461 tatgcgagca gcaagcatga caacgtcttt gataagtacc tcttgtttgt attcttctga
    11521 atttatgtcc atttcatcac cattgtttat aagaatttaa tactcattat agtttataca
    11581 ctataaaata accacatgag ctttttgata agttgttatt gattttaaaa aactaatatt
    11641 t
//
LOCUS       DALAGF010000084        11578 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.084, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000084 DALAGF010000000
VERSION     DALAGF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11578
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.084"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(143..1399)
                     /locus_tag="O2692_002068"
     CDS             complement(143..1399)
                     /locus_tag="O2692_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HCZ4299689.1"
                     /translation="MIDNFMQVLKLIKEKRTNNVVKKSDWDKGDLYKTLVHDKLPKQL
                     KVHIKEDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLFHPEGEGIYLSL
                     NQGWSKISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKND
                     YPSGYSHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKTSYDSLVNSIDE
                     IQEDSEIEEIRTAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIEKSNKENKLTGK
                     VGEKLALNYFNELIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDKAVEK
                     FIEIKTSTSSSIEEPFFMSLNEMFAMKEYKQKYLILRIFNVSGKEPQFYFIDPYANYS
                     EFKDVDDLIDKVFNVEAIQYKVFGEK"
     gene            complement(1721..2200)
                     /gene="rlmH"
                     /locus_tag="O2692_002069"
     CDS             complement(1721..2200)
                     /gene="rlmH"
                     /locus_tag="O2692_002069"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCZ4299690.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(2568..4886)
                     /gene="adsA"
                     /locus_tag="O2692_002070"
     CDS             complement(2568..4886)
                     /gene="adsA"
                     /locus_tag="O2692_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HCZ4299691.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQHTPMKAHAVTTIDK
                     ATTDKQQVPPTKEAAHHSGKEAATNVSASAQGTADDTNSKVTSNAPSNKPSTVVSTKV
                     NETRDVDTQQASTQKPTHTATFKLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDINKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSKSGKDTQPIGDDKVMDPA
                     KKPAPGKVVLLLAHRGTVSSGTEGSGRTIEGATVSSKSGKQLARMSVPKGSAHEKQLP
                     KTGTNQSSSPEAMFVLLAGIGLIATVRRRKAS"
     gene            complement(5114..5914)
                     /locus_tag="O2692_002071"
     CDS             complement(5114..5914)
                     /locus_tag="O2692_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCZ4299692.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAAHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(6304..7092)
                     /locus_tag="O2692_002072"
     CDS             complement(6304..7092)
                     /locus_tag="O2692_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCZ4299693.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPTSILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGAEYQLSEISSDSVKYEQTYDDFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(7093..8427)
                     /locus_tag="O2692_002073"
     CDS             complement(7093..8427)
                     /locus_tag="O2692_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299694.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLNSDFILFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(8420..10246)
                     /gene="walK"
                     /locus_tag="O2692_002074"
     CDS             complement(8420..10246)
                     /gene="walK"
                     /locus_tag="O2692_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCZ4299695.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(10259..10960)
                     /locus_tag="O2692_002075"
     CDS             complement(10259..10960)
                     /locus_tag="O2692_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4299696.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
BASE COUNT         3282 a         2250 c         1716 g         4330 t
ORIGIN      
        1 catgccacga aattagcatc atgctagcaa gttaagcgaa cactgacatg ataaattagt
       61 ggttagctat atttttttac tttgcaacag aaccttctat ttttattaga tcacaatttt
      121 attttagctc ttgttcaagt aatcattttt cgccaaaaac tttatactga atagcttcta
      181 cattaaatac tttgtcaatg agatcatcta catctttaaa ttcagaataa tttgcatatg
      241 gatctataaa ataaaattgt ggttctttac cggaaacatt aaatattctt aatattaaat
      301 atttctgctt atattctttc atagcaaaca tttcatttag cgacataaaa aatggttcct
      361 caatactaga agatgtagat gttttaattt caataaattt ttctacagct ttatctgtat
      421 ttgttggatc aaaagctact aaatcatagc catgaccgtg ttgagagcct ggattatcat
      481 ttaaaatatt cctaaactgt tctttcttat cttcgtctat tttattatca attagctcat
      541 taaagtaatt tagcgctaat ttttctccaa ctttaccggt taatttattc tctttatttg
      601 atttttcaat ttctgaatca tttttagtag tctttgatac acctttttta tattttggaa
      661 ttattccttt aggtgcttcc acttccttga gtgtcttatc tttttgtgct gttctaattt
      721 cttcaatttc gctgtcttcc tgtatttcgt ctatgctatt gaccaagcta tcataggatg
      781 tttttgtaac ttttgaagct aattcattaa atagttctaa aaatttcttt aaatcctcta
      841 gcatatcttc ttctgtgaat ccttcattca aatcataata tttgaatctt attgatccat
      901 gagaatatcc tgatggataa tcatttttta aatcataaga tgaatcttta ttttctgcgt
      961 aataaaatct tccagtatta aattcatttg atgtaatata tttattgagt tcggaagata
     1021 aagttaatgc tctttgtttt gcagcatttt tatcccgcgg aaacatatca cttatctttg
     1081 accatccttg attcaaagat aagtatatgc cttctccttc cggatgaaaa agatatacca
     1141 aataatatcc atcctttgtt tcttttgtta tattctcatc atatattgaa atccaaggaa
     1201 ctttactata gttcccagta gcaaccttcc ctacaactga atatttatct tcttttatat
     1261 gcacttttaa ctgcttgggt aacttatcat ggactaaagt tttatataga tcacctttat
     1321 cccaatcaga ttttttaact acattattgg tacgtttctc tttaattaat ttaaggacct
     1381 gcataaagtt gtctatcatt tgaaattccc tcctattata aaatatatta tgtctcattt
     1441 tcttcaatat gtacttattt atattttacc gtaatttact atatttagtt gcagaaagaa
     1501 ttttctcaaa gctagaactt tgcttcacta taagtattca gtataaagaa tatttcgcta
     1561 ttatttactt gaaatgaaag actgcggagg ctaactatgt caaaaatcat gaacctcatt
     1621 acttatgata agcttcttaa aaacataaca gcaattcaca taaacctcat atgttctgat
     1681 acattcaaaa tccctttatg aagcggctga aaaaaccgca tcatttatga tatgcttctc
     1741 ctcgcataat cttaaatgct ctgtacactt gttcaattaa cacaacccgc atcatttgat
     1801 gtgggaatgt cattttgctg aatgatagtg cgtagttact gcgttgtaag acgtccttgt
     1861 gcaggccgtt tgatccgcca atgacgaaaa caaagtcgct ttgcccttgg gtcatgcgtt
     1921 ggttcaattc ttgggccaat ccttcggaag atagcatctt tccttgtatt tctaatgtaa
     1981 tgactgtgga ttgtggtttg attttggcta gtattcgttg gccttctttt tcttttactt
     2041 gctcaatttc tttgtcactc atattttctg gtgctttttc gtctggaact tctatgatgt
     2101 ctatcttggt gtatgggcct aaacgttttt catattctgc tatggcttgc ttccaatatt
     2161 tctcttttag tttccctaca gctaaaatgg tgattttcat gtcgtttggt cctccaaatt
     2221 gttatcaact ttccagttat ccacaagtta ttaacttgtt cacactgttc cctcttatta
     2281 taccaatatt ttttgcagtt tttgatattt tcctgacatt tatgcccgat ttttagtgta
     2341 taacctgtgt atttgtggat aactctgtgg gcattgtggg tatttctctt taaattgtac
     2401 acaattctgg ataactatcc ccatttctgt ggataaacac cttgatgtct tattacttat
     2461 cctcgttttt atatcccata tttatcaacg gttatcgctt tttttcataa aaaaacacta
     2521 ccgtacctct tatttaagaa atacagtagt attattttca atatatttta gctagctttt
     2581 ctacgtcgta cagtcgcgat taaacctata cctgctaata atacaaacat cgcttctggg
     2641 cttgaacttt gattagttcc agtttttggt aactgtttct catgcgcgct acctttaggc
     2701 actgacattc tagccaattg tttcccactc ttgcttgata cagtagctcc ttctattgtg
     2761 cgaccagaac cttctgtacc gctactaaca gttcctctat gcgctagcaa caatacaact
     2821 ttacctggag ctggtttttt cgctggatcc atcactttgt cgtcaccaat tggttgtgta
     2881 tctttaccag acttactacc tttgctacct tgttgtcctt tagctggttg ttcacttact
     2941 gctggtttac ctaataacat acgttgtggt tctgtcgtat catacttagc taagttagct
     3001 gtttttaaat aacttgctag tacttgatct aatgaaatac cttcttctct aggaccaccg
     3061 aacatactat atccgtcgcc acctgatgct gtgaagtcat tcatcgttac gtgatataca
     3121 cgttttaaat caatattttc aaacttacct gtctctttat ttaaaatttg aatagcatta
     3181 attcgtttgc cagacggttt atttatatca tagtaaacac ggattgaatc agagatatgt
     3241 agtaaaccgc cattcgctgt taacactgtc ttaccgtcct tttgtgttgt tggtgcgcct
     3301 aaactatgtt cgaaagccgt ccagacgtct gaacctttta catcaatttg cgcaatcgta
     3361 tttccaaatg gtaatactga gattaaatca tagcgtgtca ccttaccttt tgcgatagag
     3421 gcacgaatac ctccaccatt tgtcacggca aagtcagtct ttttagagaa attcttaacg
     3481 ccatacgctt ccatagcatc tgcaatcgcg tttcctaaat ttgtttcacg cgttctaacg
     3541 tcatctcttt ctcctttgaa atcaatggta ttgtttggaa taattacctc tgcagtttgt
     3601 gctctaaatg tttgatcagc ttgattaatt tgttcagcta atgctttgtt cggtgttaca
     3661 ttttcaacgt ctttaacatt aatcaatgac ggtttaatat tcgatacctc tccattgcga
     3721 taattaaatg taatcttacc gatattcgca agtgctgtac ctgtttgtgc caatgcatca
     3781 ttgttataaa tttgaccatt ttgaagtact gtatgtgaat gaccatcaat aactgtaata
     3841 cgtttcttca attgtggatt ttgacttaat tgtttcacta agtaatcacc acgccatgtt
     3901 tcttgtgttg aaggatcaat tcctaaatgt gatataacaa caaatgtatc tacgtcttta
     3961 taaatacgca tcatttccgc tgtcacactt tgtaatggat ctctaaattc aacgccttta
     4021 atgccttcag gtcttgtttt cgtctttgtt tctggtgtcg ttacaccaat aattccataa
     4081 cgaataccat tttttgttac aatcgttgaa ggcttaaacg cgcgttttcc atctttataa
     4141 acgttagtac ttagcatcgg gaagtctaac ataccctcta actttttcaa ctgatcgtat
     4201 ccaaagtcaa attcatggtt accgactgcc atagcatcat aacctactgc attcattgct
     4261 ttagccattt cttcaccttt agactggttt gaaagtggta aaccttggaa ggcgtctcct
     4321 gcgtctaaca ttaaatcagg cttttcttgt tcttttactg tttttaattt agccataccg
     4381 atgacacgcc ctttttcttc ggctagtcgg ccatggatat catttgtatg taatatttta
     4441 tgtgttgttg tttgtggtgc attagcagca aacattcgtg gtgaaagtga tgctgtttta
     4501 gcatttgata atttgaacgt tgctgtgtga gttggttttt gtgttgaggc ttgttgtgta
     4561 tctacgtcgc gtgtttcgtt tacttttgtt gaaactactg tagatggttt gttagatggt
     4621 gcgttggatg ttactttgct gtttgtatca tcagctgttc cctgcgctga tgctgatacg
     4681 ttggttgccg cttctttgcc agaatgatga gccgcttcct ttgttggcgg tacttgttgc
     4741 ttatctgttg ttgctttgtc tatcgttgtt actgcatgtg ctttcattgg tgtatgctgc
     4801 tcagccgcgt tcgagacttg ccataatccc attacactaa aaagcaaaac gagccataca
     4861 cttgttttaa gtaataaagc tttcatttcg ttccctccat taactgtcat acaccaatat
     4921 attatcataa tccttatatt aaaatgattg attttatgta aattatttat aaattacatt
     4981 taatatcgaa ttattttaca catttcaatc aagccgattc aactcaacaa cagccatttc
     5041 ccaccaacaa cataaaaaag cacccgataa agtcgtctca cagcaatgcg gaactttatc
     5101 ggatgactct catttatatt gtatatattg gcgttggaat agctttatcc gtatcacata
     5161 gcaatacttc tttttccgta tcaatatcgt gttcgttcaa tacttggcca acactcatac
     5221 gcgccaaatc tttcatgtta ttgtcttgtg ataaatgcga taggtaaata cgtttcgtgt
     5281 tacctgtaat cacgtctgtc attgcatgag ccgcatcctc attagataca tgacccatat
     5341 cgcctaaaat acgttgtttc gtcttccatg gataacgaca cattctcaac atatcgacgt
     5401 catgattact ctcaaaaata aacgcatcgc tgccacgtat catacctttc atacgatcag
     5461 acacgtaacc cgtatccgtt aaaatcgtaa acttcttata gttattatgg aaaatataaa
     5521 attgcggatc tattgcatca tgtgacacgt taaacgattc aacatcgaaa cctgcaatag
     5581 attttgtttc ataaggatta aaaatgaatt tctgatccat agggatgcga ctatctttct
     5641 tttcaattgc ctgccaagtc ttttcattcg cataaattgg caattgatat ttacgcgcca
     5701 aaacacctaa tcctttaata tgatcaatat gttcatgggt tactaaaata ccatttaaat
     5761 cttgaatatt acggtcaatt tgactaaaca attcttccat tttctttcca gtcaaaccaa
     5821 catcaactaa tagactacct ttttcatttt ctacaaaagt ggcgttacct gtactaccac
     5881 ttgctaatac actcatgcgt atcaagcggc tcatcctttc tctttaaaca gtcaatctct
     5941 ctatatgact gcaatataca tcagtgcaaa tatacgattt tccctacaga aactatcagt
     6001 atattagctg ttttgtatac ctttcctatt atgtaacttt agttcatcga ccggtttctt
     6061 atatttaaat tttgataatc acgtgcaatg aatttcttaa ttattataac aatgattaga
     6121 caaatactaa agtttttaaa ataacagtta cactaatttt acaatgcggt tctacttaaa
     6181 atatgaataa atgcgcacgt tggagacgca aaaacatcag ccataataaa ccgtaccagc
     6241 aaaaaagcta gcaccgcttc tcacatatga tgagcttgca atgctagctt attacgattc
     6301 atattaatga ttaataattt tagggttatt atttgtcgct tcgacatagt aagtattcgt
     6361 tttatcctta ccgtcatgct tcactttaat ttcccagttt ggttgtaaca attgaacatt
     6421 cgtttcattc acgactgagt agtaaccgag tctagcatta atgacttcat cacctttttt
     6481 caagtaacga ttataatata atgcctcgat tgcttttctc acaccaatca cttgatgctt
     6541 cttatctgca ccatctgtgg gcttaatgtc atccatcatt gattgtttat aactagtcgc
     6601 tttgttatct tctatattaa agttcaacat cgctttactg ttatttaaaa tcggaaaatc
     6661 atcatacgtt tgttcatatt ttacagaatc tgaactaatc tcgcttaatt gatattcagc
     6721 acctttaaat acgcgcttgt taacataatc tttcaaatct tttaagttat tgtcactgac
     6781 ctttaccgat tgactaatgt tcgcattcaa taattttcca ccatctgatg tggtcaaatc
     6841 cgaatcgcct ttagctttag aactaaagtc ttttgatcgc cctgtaattt gctctaattt
     6901 tataccttta actgatttat tcaatatact agtcggtact ttaatttctt cttgctgaaa
     6961 attaacttca ttgttactct cgacttcatt aatgtgtgag cgattgactt tattaacata
     7021 aatcgatact aacacgatgt tgacaagaat aaacacgaaa atgaaaagtg tctttgtcag
     7081 tttccagttc atttattcaa gcctcccatc gttataaaca taccattcgc catcatattc
     7141 tacataccaa cgcggtacga gttcactgtt aatctgcact tcaatgtgat tatgatctga
     7201 attatcctgc atttcgtaac cgatagcgat gtttgttact ttttcaaaat taatatcact
     7261 attgttcgct aagcttgaac gtacagactc taatttcggc aacgatttat tatcctcact
     7321 atttaaaaca acgtcggtgc gtaataacga acgacgatag tcaaagacgc ctttttcacc
     7381 ccaagtgact tgaatttgat tagaaccttc tttattaaac gttggataac cattaaggaa
     7441 acgttgatat gttaactcgc ctgactgatt attcgtacta aacaatctaa agtcttcgtt
     7501 taagaaacca ccatgaccat taataaaatc aaaggttcct ggaatcgttt cttccatttt
     7561 gctggaactc gcttcatctt cggacaggtt tttataatga tatttttcat ttttatcgtt
     7621 atagtttgcg acacctgtat tattgttgta ggttgtaaca ccactctttg aactacgaac
     7681 gatggttgaa tcgtcaaata gtatagcatt cattttctca acactaatcg tgttaaatac
     7741 catgcgatat gtttttaact tttcaggttt acttggtgca aaaacatgcg tcgtacgatc
     7801 aattgtatct ttgtttgtga tgatatctgt gtatggttgc atatcttttt tcactgctgc
     7861 taatgcatct aaaaaatgat catttttcgt tgtagttgtt aattttacgt aatcgtggcg
     7921 atctttgctt atagcataaa gcacgatatt atcatcagca tcatgatcta tgaccaaacg
     7981 attgaaattg aaatgatttg gtactttcgc attcatgttc agtacttgac caagatatgt
     8041 tgataacggt aaatcatacg taaaatcgaa taatataaaa tcactgttca aatcaggaat
     8101 catcaagtta tgatcacgac gaacatgttc cacggacttc acttctttat ttttcaaagg
     8161 tttcgtcagt ttattcacat tagataccgt cgcaatcgtt ccttctggat gatcattttt
     8221 cgaatgaata atctgaaatg gcgtaatagt tgtatccatt ttggctgtca taggtgtcgt
     8281 taaaggtttc gtttcactct tcttactatc tgtattgtcg acatttgcaa tatcaggaga
     8341 aaagttccat accatatatg tcaatacgac actcatcaag acgagtagtg ctaaaatgac
     8401 agatttaata tgctccttat tattcatccc aatcaccgtc ttcaatgact tcacatggaa
     8461 gtgtgataaa gatagatgta ccttgacctt ctacactgtt tgcccaaata cgaccattgt
     8521 gcgcttccac aatctctttc gaaatggcta gtcctaatcc agtaccaccc attttacgcg
     8581 tacgtgcctt atctacacga tagaatcggt cgaatatctt atcgacttta ttgataggaa
     8641 taccaatgcc attatcttta atacgaatcg tcattcgatt ataaagtgga ttttgtttca
     8701 cgtggaactc gacacgttta tcgcctctag aatatttcat cgcatttgta atgacattat
     8761 caaatacttg cgtcatttta tcaggatcaa attctgtgaa aatcgtcttt ttcggaatat
     8821 ctcgaataaa tgttgtatct ttcgcagaca tttcatgtcg attaataatt ttattaatga
     8881 acatgttaaa gtcgataatt tctttgttga tttgatcaga ctcattatcc attttagata
     8941 actgtagcaa gtcattgacc agtcgaatca ttcgttctgt ttcttcacgg gtaacagata
     9001 aaaattgtgg cgcaagttcc tcatctttcc atgcaccttc ttcaagtgct tcaatgtaac
     9061 tattcataga agttaaaggt gtacgtaact catgtgatac attggcaaca aattcacgac
     9121 gctcacgttc aacttgttgt tgttcagtta cgtcatgtaa cacagcgata taaccagtta
     9181 caaatcctgt ttcctgcaca atcgtactaa agttaacacg tgcgattaga ccttcttctt
     9241 catttaaatc taataagaaa ctatcattat tctcttgaat ttcttccagt ttaaattcat
     9301 cttcaagact taatacactt aacatgtaat atccgatgat gtcttctttc gccataccaa
     9361 gcatcttgag tgccatatca ttgacgatac gaatacgtcc acggcggtct gttgcaataa
     9421 taccatcact catatgggtg ataactgagt ccagtctacg tttctcactt tcagtattag
     9481 cctgcgcttc ttgtacacgt ttagacaagt tattaaatgc taaagctaat tcgccaattt
     9541 catcattacc ataaatcttc acacgttgcg tatagttacc tctggacatt tcgaccgtct
     9601 ggttacgcat atcggtgatt ggtttggtaa tcgttcgcgc tataaagaat cctaggatga
     9661 ctgtgattaa taatgaaata gctgtaccaa caatgaatat ttgatttata ttatttaatt
     9721 ggttataaac gtcattaatt tttgattcga tataaatatt accaattacc tttttatcga
     9781 ctttaactgg gatattatat acccagacac ggtccttacc accgccataa tcttttaaaa
     9841 ttaaatgatc gtttgattgt cctagtgata gtgctttttg gacagaacta tcattcgctt
     9901 tttgattgat tagactacgg ttagactgct tcgtcgtcgc aataataatt tggtctttat
     9961 ctataaaacg aatttctcca atttcttgac ggttggcata ctcacttaat aaattttgaa
    10021 tatctttttg tgcatttacg gagccctttt cgtcatatac tttttcaata ctaatttcta
    10081 attgtttcgc gtactgcgta atattcttct taaaattatc aagcagctct ttttcaaggt
    10141 tatttgtaaa atataacccg ataatttgca taccaatgat aatcagtaat acataaacaa
    10201 ttacaagttt agtatgaagg gattgtagtt gttttagcca cttcattcgt ttcgacctct
    10261 actcatgttg ttggaggaaa tatccaacgc ctctacgcgt cacaatatat tcaggatgtg
    10321 acggatcatc ttcaatcttt tcacgtaaac gacgaatcgt tacatcgacc gtacgtacat
    10381 cgccaaagta atcatagccc catactgttt gtaataaatg ttcacgtgtc attacttgtc
    10441 ccatatgttt tgataaataa tggaacaatt caaattcacg atgtgttaat tcaatatctt
    10501 cgccacgttt tttaatagaa tatgcgtctg gataaatcac aatatcttta attgtgattt
    10561 cattcgttac atttccagtg tcttgtgctg gttgtgagta atgacgacgt aagttcgctt
    10621 tcacacgtgc gattaattca cgcgtactaa acggtttcgt tacatagtca tctgcaccta
    10681 gttctaaacc aagcacttta tcaatttctg aatctttagc agtaagcatt attattggca
    10741 tttcgtattt tttgcgcact tcacgacata cttccatacc atcacgacca ggtaacatga
    10801 tatctagtaa tacgatgtct ggttcttctt cataaattaa gtcgactgca tcattaccat
    10861 cgtatgcaca gtacacatcg tatccttctt tttttaagtt aaattctaaa atatcagcaa
    10921 tcggtttttc atcatcaact acaacaactt ttctagccat ttgcataaac ctcttttctt
    10981 aaatcattaa tattatttta tcattttttg ttctttatcg tttatgtttg tgtaaaaaat
    11041 cacaggatgt cgcttactat ttttgttatt gtgcgcattt tccgtcttat aacgtatagt
    11101 aacctattat ataatttacc atttataagc tattaattct attttccaat ataaacttat
    11161 atattactat tttttattag tttctcacgg cggattgtga caatgatatt taacacaatg
    11221 atgaggtctc tattccaata ataaaattag gataatggca acttctagtt gcacgtcatt
    11281 gctcagaaaa taaatatcat tattcgttat tcgttacttt aacaattgat gttatggagg
    11341 actggtggca tatcacaacg aagaaatatg ttgacagtag catatgtttg tacgtctaat
    11401 aaagatttta ttgtctacgt ttatctttca tatccaacca ctaagttact tgaggttcat
    11461 acattttaaa gaaaatggac gaacagataa atcaatatga ttatcattcg tccatatatc
    11521 ttttattaat ataatattaa acgcaaaaaa atgcctatcc tcttggatag gcatttgt
//
LOCUS       DALAGF010000085        11462 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.085, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000085 DALAGF010000000
VERSION     DALAGF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11462
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.085"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            397..2085
                     /gene="ilvD"
                     /locus_tag="O2692_002076"
     CDS             397..2085
                     /gene="ilvD"
                     /locus_tag="O2692_002076"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCZ4299697.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLTLPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            2113..3882
                     /gene="ilvB"
                     /locus_tag="O2692_002077"
     CDS             2113..3882
                     /gene="ilvB"
                     /locus_tag="O2692_002077"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HCZ4299698.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            3882..4136
                     /locus_tag="O2692_002078"
     CDS             3882..4136
                     /locus_tag="O2692_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCZ4299699.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            4273..5277
                     /gene="ilvC"
                     /locus_tag="O2692_002079"
     CDS             4273..5277
                     /gene="ilvC"
                     /locus_tag="O2692_002079"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCZ4299700.1"
                     /translation="MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVV
                     IGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNA
                     LAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQGASGQA
                     RNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGY
                     QPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVKENMKA
                     VLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK
                     "
     gene            5307..6836
                     /locus_tag="O2692_002080"
     CDS             5307..6836
                     /locus_tag="O2692_002080"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCZ4299701.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            6839..7885
                     /gene="leuB"
                     /locus_tag="O2692_002081"
     CDS             6839..7885
                     /gene="leuB"
                     /locus_tag="O2692_002081"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCZ4299702.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            7899..9269
                     /gene="leuC"
                     /locus_tag="O2692_002082"
     CDS             7899..9269
                     /gene="leuC"
                     /locus_tag="O2692_002082"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCZ4299703.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            9270..9842
                     /gene="leuD"
                     /locus_tag="O2692_002083"
     CDS             9270..9842
                     /gene="leuD"
                     /locus_tag="O2692_002083"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCZ4299704.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYVEIEVDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            9857..11125
                     /gene="ilvA"
                     /locus_tag="O2692_002084"
     CDS             9857..11125
                     /gene="ilvA"
                     /locus_tag="O2692_002084"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HCZ4299705.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
     gene            complement(11335..11449)
                     /gene="rrf"
                     /locus_tag="O2692_002085"
     rRNA            complement(11335..11449)
                     /gene="rrf"
                     /locus_tag="O2692_002085"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3867 a         1894 c         2211 g         3490 t
ORIGIN      
        1 atctttgaaa cagattatct attcaaagtt aattgtaaga aaatttaaaa tatttgttga
       61 catactaaag cagatatagt aaattaaatt tatcaaattt ttagacaatt ctaactatta
      121 aagtgatata taccattcac ggaaggagta taataaaatg cttaatcaat atactgaaca
      181 tcaaccgaca acttcaaata ttattatttt attatactct ttaggactcg aacgttagta
      241 aatatttact aaacgcttta agtcctattt ctgtttgaat gggacttgta aacgtcccaa
      301 taatattggg acgttttttt atgttttatc tttcaattac ttatttttat tactataaaa
      361 catgattaat cattaaaatt tacgggggaa tttactatgc gaagcgacat gatcaaaaaa
      421 ggagatcacc aagcaccagc aagaagtctt ttacatgcca cgggcgcgct aaaaagtcca
      481 actgatatga acaaaccatt tgtagctatt tgtaactctt atattgatat tgttcctgga
      541 catgttcact tgagagagct tgcagatata gctaaagaag caattagaga agccggtgcc
      601 attccatttg aattcaatac aattggtgtt gatgatggaa tagctatggg acatatcgga
      661 atgcgatatt ctctaccatc acgtgaaatt attgcagatg cagctgaaac tgtaattaac
      721 gctcattggt ttgacggcgt attttacatt cctaattgtg acaagattac acccggtatg
      781 attttagcag ccatgaggac aaacgtacca gctatctttt gctctggtgg accaatgaaa
      841 gctggcttat ctgcacatgg aaaagcatta acactttcat caatgtttga agcagtcggc
      901 gcatttaaag aaggatcgat ttctaaagaa gaatttttag atatggaaca aaatgcctgc
      961 cctacttgtg gttcatgtgc tgggatgttt actgcaaatt caatgaactg tttgatggaa
     1021 gttttaggtc taacattacc atacaacggt actgcacttg cagtcagtga tcagcgacga
     1081 gaaatgattc gccaagcagc ttttaaatta gttgaaaata ttaaaaatga tttaaaacca
     1141 cgtgatatcg ttactcgcga agcaattgat gatgcatttg cacttgatat ggctatgggt
     1201 ggttcaacaa acacggtact gcatacgtta gccattgcca atgaagctgg tattgattat
     1261 gacttagagc gcattaatgc tattgccaaa cgcacgccat atttatcaaa aatagcacct
     1321 agttcatcgt attcaatgca tgatgtgcat gaagctggtg gcgtcccagc aattattaat
     1381 gaattgatga agaaagatgg cacgttacac ccagatagaa tcacagttac tggcaaaacg
     1441 ttacgtgaaa ataacgaagg caaagaaatt aagaactttg atgtcattca ccctcttgat
     1501 gcaccatatg atgcacaagg cggtttatct atcttatttg gtaatatcgc ccctaaaggc
     1561 gcagttatta aagttggcgg cgttgatcca tctatcaaaa catttactgg gaaagcaatt
     1621 tgtttcaatt cgcatgatga agctgttgaa gcaatagaca atcgtaccgt tcgtgcaggc
     1681 cacgtcgttg tcattagata tgaaggacct aaaggtggac caggtatgcc tgaaatgtta
     1741 gcacctactt cctctattgt tggtcgcggc ttaggtaaag atgttgcatt aattactgat
     1801 gggcgttttt ccggtgccac aagaggtatt gcagttggtc atatttcccc tgaagctgca
     1861 tctggtggac caattgcctt aattgaagat ggtgatgaga ttactattga tttaacaaat
     1921 cgtacattaa acgtaaacca gcctgaagat gttctagcgc gtcgccgaga atctttaaca
     1981 ccatttaaag cgaaagtaaa aacaggttat ctagctcgtt atactgccct agtaactagc
     2041 gcaaatacag gtggcgtcat gcaagtccct gagaatttaa tttaatttat ttttatattg
     2101 gagatggtta aaatgtctaa aactcaacat gaagtaaacc aaaatattga ccctttaaaa
     2161 atggctgaat cacttgaacc tgaacaacta aatgaaaaaa ctttaaatga tatgcgttca
     2221 ggatcagaag tgctagtaga agctctactt aaagaaaatg tggattattt attcggttat
     2281 cctggtggtg ccgtactacc tttatatgac acgttttatg atggtaaaat caaacatatt
     2341 ttagcaagac acgaacaagg tgctgttcat gctgcagaag gttatgcacg tgtatctggt
     2401 aaaactggcg tcgttgtagt tacaagcggt ccaggtgcaa ctaatgtaat gacaggtatt
     2461 acggatgcac attgcgactc tttacctcta gttgtattca ctggacaagt tgctacacca
     2521 ggcattggta aagatgcatt ccaagaagcg gatattctat ctatgacttc accaattaca
     2581 aaacaaaatt atcaagtgaa acgtgttgaa gatatcccta aaatcgtaca cgaagctttc
     2641 catgtagcta attctggacg caaaggtcct gtagtgattg attttccaaa agatatgggt
     2701 gttttagcta caaatgtgga tttatgcgac gaaatcaata ttccaggtta tgaagttgtt
     2761 acagaaccag aaaataaaga cattgacact ttcatctcac ttttaaaaga agcgaaaaag
     2821 cctgtcgtat tagccggcgc aggtattaat caatcaaaat caaatcaatt attaacacag
     2881 tttgttaata aacatcaaat cccaacagtt actactttac taggtctagg tgctgtacct
     2941 tatgaggata cactattttt aggtatggga ggaatgcatg gttcttatgc tagtaacatg
     3001 gcattaactg agtgtgattt actcattaat ttaggtagcc gcttcgatga tagattagca
     3061 agcaaacctg atgcctttgc acctaacgcc aaaattgtac atgtagatat tgatccttca
     3121 gaaatcaata aagttattca tgtagattta ggtattattg cagactgtaa aagattttta
     3181 gaatgtttaa atgataaaaa tgttgagact atagaacaca gtgactgggt taaacattgt
     3241 caaaataata agcagaaaca cccatttaaa cttggtgaag aagatcaagt attttgtaag
     3301 ccacaacaaa caatcgaata tatcggcaaa attacaaatg gtgaagcaat tgttactaca
     3361 gacgtgggac aacatcaaat gtgggcagct caattttatc catttaaaaa tcacggacaa
     3421 tgggttacaa gcggtggttt aggaacaatg ggattcggta ttccttcgtc aattggtgcc
     3481 aaattagcta atcctgataa aacagtcgta tgtttcgtcg gtgacggtgg tttccaaatg
     3541 acaaaccaag aaatggcact tttacccgaa tatggtttag atgtcaaaat cgtactaatc
     3601 aataatggaa cattaggtat ggttaaacaa tggcaagata agttctttaa tcaacgcttc
     3661 tcacactcag tatttaatgg tcaacctgat tttatgaaaa tggcagaagc atatggcgtc
     3721 aaaggtttct taatcgataa gccagaacaa ctggaagaac aattagatgc agcgtttgct
     3781 tatcaaggac cagctttaat tgaggttcgt atttccccta ctgaagctgt aaccccaatg
     3841 gttccgagtg gcaaatcaaa tcatgaaatg gagggcttat aatgacaaga attcttaaat
     3901 tacaagttgc ggatcaagtc agcacgctaa atcgaattac aagtgctttt gttcgcctac
     3961 aatataatat cgatacatta catgttacac attctgaaca acctgggatt tctaacatgg
     4021 aaattcaagt cgatattcaa gatgatacat cacttcatat attaattaaa aaattaaaac
     4081 aacaaattaa tgttttaacg gttgaatgct acgaccttgt tgataacgaa gcttaatttt
     4141 aagacaaagg caatgatgcg ctaattagtt atagatatat cataggctgc tagttaacat
     4201 ctgccactat tacaaagtta tatttcagaa ttttcgaaac acaaaatatt taattatttg
     4261 gaggaattta ttatgacaac agtttattat gatcaagatg taaaaacgga cgctttacaa
     4321 ggcaaaaaaa ttgcagtagt aggttatgga tcacaaggtc acgcgcatgc acaaaactta
     4381 aaagacaatg gatatgatgt agtcatcggc attcgcccag gtcgttcttt tgacaaagct
     4441 aaagaagatg gatttgatgt gttccctgtt gcagaagcag ttaagcaagc tgatgtaatt
     4501 atggtgctat tacctgatga aattcaaggt gatgtataca aaaacgaaat tgaaccaaat
     4561 ttagaaaaac ataatgcgct tgcatttgct catggcttta acattcattt tggtgttatt
     4621 caaccaccag ctgatgttga tgtattttta gtagctccta aaggaccggg tcatttagtt
     4681 agacgtacat ttgttgaagg ttctgctgta ccatcactat ttggtattca acaaggcgct
     4741 tcaggtcaag cacgtaatat tgctttaagt tatgcaaaag gtattggtgc aactcgtgca
     4801 ggtgttattg aaacaacatt taaagaagaa actgagacag atttatttgg tgaacaagca
     4861 gtactttgcg gtggtgtatc gaaattaatt caaagtggct ttgaaacatt agtagaagcg
     4921 ggttatcaac cagaattagc ttattttgaa gtattacatg aaatgaaatt aatcgttgat
     4981 ttgatgtatg aaggcggtat ggaaaatgta cgttactcaa tttcaaatac tgctgaattt
     5041 ggtgactatg tttcaggacc acgtgttatc acaccagatg ttaaagaaaa tatgaaagct
     5101 gtattaactg atatccaaaa tggtaacttc agtaatcgct ttatcgaaga caataaaaat
     5161 ggattcaaag aattttataa attacgcgaa gaacaacatg gtcatcaaat tgaaaaagtt
     5221 ggtcgtgaat tacgcgaaat gatgcctttt attaaatcta aaagcattga aaaataagat
     5281 agacctacaa tgaggagttg ttaaatatga gtagtcatat tcaaattttt gatacgacac
     5341 taagagacgg tgaacaaaca ccaggagtga attttacttt tgatgaacgc ttgcgtattg
     5401 cattgcaatt agaaaaatgg ggtgtagatg ttattgaagc tggatttcct gcttcaagta
     5461 caggtagctt taaatctgtt caagcaattg cacaaacatt aacaacaacg gctgtatgtg
     5521 gtttagctag atgtaaaaaa tctgacatcg atgctgtata tgaagcaaca aaagatgcag
     5581 cgaagccggt cgtgcatgtt tttatagcaa catcacctat tcatcttgaa cataaactta
     5641 aaatgtctca agaagacgtt ttagcatcta ttaaagaaca tgtcacatac gcgaaacaat
     5701 tatttgacgt tgttcaattt tcacctgaag atgcaacgcg tactgaatta ccattcttag
     5761 tgaaatgtgt acaaactgcc gttgacgctg gagctacagt tattaatatt cctgatacag
     5821 tcggctacag ttaccatgat gaatatgcac atattttcaa aaccttaaca gaatctgtaa
     5881 catcttcaaa tgaaattatt tatagtgctc attgccatga cgatttagga atggctgttt
     5941 caaatagttt agctgcaatt gaaggcggtg cgagacgaat tgaaggcact gtaaatggta
     6001 ttggtgaacg agcaggtaat gcagcacttg aagaagtcgc gcttgcacta tacgttcgaa
     6061 atgatcatta tggtgctcaa actgctctta atctcgaaga aactaaaaaa acatcggatt
     6121 taatttcaag atatgcaggt attcgagtgc ctagaaataa agcaattgtt ggccaaaatg
     6181 catttagtca tgaatcaggt attcaccaag atggcgtatt aaaacatcgt gaaacatatg
     6241 aaattatgac acctcaactt gttggtgtaa gcacgactga acttccatta ggaaaattat
     6301 ctggtaaaca cgccttctca gagaagttaa aagcattagg ttatgacatt gataaagaag
     6361 cgcaaataga tttatttaaa caattcaagg ccattgcgga caaaaagaaa tctgtttcag
     6421 atagagatat tcatgcgatt attcaaggtt ctgagcatga gcatcaagca ctttataaat
     6481 tggaaacact gcaactacaa tatgtctcta gcggccttca aagtgctgtt gttgttgtta
     6541 aagataaaga gggtcatatt taccaggatt caagtattgg tactggttca atcgtagcaa
     6601 tttacaatgc agttgatcgt attttccaga aagaaacaga attaattgat tatcgtatta
     6661 attctgtcac tgaaggtact gatgcccaag cagaagtaca tgtaaattta ttgattgaag
     6721 gtaagactgt caatggcttt ggtattgatc atgatatttt acaagcctct tgtaaagcat
     6781 acgtagaagc acatgctaaa tttgcagctg aaaatgttga gaaggtaggt aattaattat
     6841 gacttataac attgttgccc tacctggtga tggaatcggt ccagaaattt tgaacggatc
     6901 tctatcattg cttgaaatta taagtaataa atataacttt aattatcaaa tagagcacca
     6961 cgaatttggt ggtgcctcta ttgatacatt cggcgagcct ttaactgaga aaaccttaaa
     7021 tgcgtgtaaa agagcagatg ctattttact gggtgcaatc ggtggaccta aatggacaga
     7081 tcctaacaat cgaccagaac aaggattatt aaaattgcgt aaatccttaa atttatttgt
     7141 aaatatacgc cccactaccg ttgtcaaagg cgctagttct ttatcacctt taaaggaaga
     7201 acgcgttgaa ggcacagatt tagttatagt ccgtgaattg acaagtggta tttattttgg
     7261 agaacctaga cattttaata atcacgaggc cttagattct cttacttata caagagaaga
     7321 aatagaacgc attgttcacg tagcatttaa attggccgct tcaagacgag gaaaactaac
     7381 atcagttgat aaagaaaatg tattagcttc tagtaaattg tggcgcaaag tcgtaaatga
     7441 agtaagtcaa ttatatccag aagtaacagt aaatcactta cttgttgatg cttgtagtat
     7501 gcatttaatc acaaatccaa aacaatttga cgtcatcgta tgtgaaaact tatttggcga
     7561 tattttaagt gatgaagctt cagtgattcc tggttcactt ggtttatcac cttctgctag
     7621 ttttagtaac gatggtccaa gattgtatga gcctattcat ggatcagcac cagatattgc
     7681 aggtaaaaac gttgccaatc catttggaat gattctatct ttagcgatgt gtttacgtga
     7741 aagcttaaat caaccagatg ctgcagatga attagaacaa catatttata gcatgattga
     7801 acatgggcaa acgacagcag atttaggcgg caaattgaat actactgata ttttcgaaat
     7861 tctatctcaa aaattgaatc actaaggggg agatgtaaat gggtcaaaca ttatttgaca
     7921 aggtgtggaa cagacatgtg ttatacggga aattgggcga accgcaacta ttatacattg
     7981 atttacacct tatacatgaa gttacttctc ctcaagcatt tgaaggactt aggcttcaaa
     8041 acagaaaatt aagacgccca gatttaacat ttgcaacact cgatcacaat gttcctacta
     8101 ttgatatatt caatattaaa gatgaaattg caaacaaaca aatcacaaca ttacaaaaaa
     8161 acgccataga ttttggggtg catatttttg atatgggttc tgatgaacaa ggtattgttc
     8221 acatggtagg acctgagaca ggacttacac agcctggcaa gacaatcgtt tgtggtgact
     8281 ctcacacagc aacacatgga gcatttggtg ctattgcatt tggaattgga acaagtgaag
     8341 ttgaacatgt tttcgcaact caaacgctat ggcaaacaaa acccaaaaac ttaaaaatcg
     8401 atattaatgg taccttacca acaggcgtct atgctaagga cattattctg catttaatta
     8461 aaacgtatgg tgttgacttt ggtacaggct atgctttgga atttactggc gaaacaatta
     8521 aaaacctttc aatggatggt cgaatgacta tttgtaacat ggctatcgaa ggtggtgcca
     8581 aatacggcat aatccaacct gatgatataa catttgaata tgttaaaggg agaccatttg
     8641 ccgataactt cgctaaatca gttgataagt ggcgtgagct atattctgat gacgacgcga
     8701 tatttgatcg tgtaattgaa cttgatgttt caacattaga accacaagtg acatggggaa
     8761 ctaatcctga aatgggtgtt aatttcagtg aaccattccc tgaaatcaat gatatcaacg
     8821 atcaacgtgc gtatgattat atggggttag aaccaggtca aaaagctgaa gacatcgact
     8881 tagggtatgt ttttctcggt tcatgtacaa atgctagact atcagatttg attgaagcta
     8941 gtcatattgt taaaggaaat aaagttcatc caaatattac agctattgtc gtaccaggtt
     9001 ctcgtacagt aaaaaaagaa gcagaaaaat taggtctaga tactatcttt aaaaatgcag
     9061 gatttgaatg gcgtgaacca ggatgttcaa tgtgtttagg catgaatcct gaccaagtac
     9121 ctgagggcgt acattgtgca tctacaagta atcgaaactt tgaaggacga caaggcaaag
     9181 gtgcaagaac acatttagta tcccctgcta tggcagcagc agcagctatt catggtaaat
     9241 ttgtggacgt aagaaaggtg gttgtttaaa tggcagcaat caaacctatt acaacatata
     9301 aaggtaaaat agtccctctc ttcaacgaca atatcgatac agaccaaatc attcctaagg
     9361 tacacttaaa gcgtatttca aaaagtggct ttggtccatt tgcttttgat gaatggcggt
     9421 acttacctga tggttcagat aatcctgatt tcaatcctaa caaaccacaa tataaagggg
     9481 cttctatttt aattactgga gataattttg gatgtggttc aagtcgtgaa catgctgctt
     9541 gggctcttaa ggactatggt tttcatatta ttattgcagg aagtttcagt gacatatttt
     9601 atatgaattg cactaaaaat gcgatgttgc ctatcgtttt agaaaaaagt gcccgtgaac
     9661 atcttgcaca atatgttgaa attgaggtcg atttaccaaa tcaaactgtg tcatcaccag
     9721 acaagcgttt ccattttgaa attgatgaaa cttggaagaa taaacttgta aatggcttag
     9781 atgacattgc aatcacccta caatatgaat cattaataga aaaatatgaa aaatcacttt
     9841 aagggagttg aatattatga cagtcaaaac aacagtttct acgaaagata tcgatgaggc
     9901 atttttaaga cttaaagata ttgtcaaaga aacaccttta caattagacc attacttatc
     9961 tcaaaagtat gattgtaaag tctatttaaa acgagaagat ttacaatggg tacgttcttt
    10021 taaattaaga ggtgcttaca acgctatttc tgttttatca gatgaagcta aaagtaaagg
    10081 tattacatgt gcaagtgcag gtaatcatgc tcaaggtgtt gcctatacag ctaaaaaact
    10141 taatttaaac gctgttatct ttatgccagt cactacacct ttacaaaagg taaatcaagt
    10201 aaagttcttt ggaaatagta acgttgaagt tgtactcact ggtgatacat ttgatcactg
    10261 tttagctgaa gctttaactt atacaagtga acatcaaatg aactttatag atccattcaa
    10321 taatgttcat acaatttctg gacaaggtac gcttgctaaa gaaatgctag aacaagcaaa
    10381 gtctgacaat gttaactttg attatctatt tgccgcaatt ggtggtggcg gtttaatttc
    10441 aggtattagt acttacttta aaacctattc acctaccacg aaaattatag gtgttgaacc
    10501 ttcaggtgca agtagtatgt atgaatctgt tgtggtaaat aatcaggtag tcacattgcc
    10561 taatatcgat aaatttgtgg acggtgcatc tgtagctaga gttggcgata ttacatttga
    10621 aattgcaaaa gaaaatgtag atgattacgt tcaagtagat gaaggtgcag tttgttctac
    10681 gattttagat atgtattcaa aacaagcaat tgtagcagaa cctgctggcg cattaagtgt
    10741 aagtgcgctt gaaaactata aagatcatat taaaggtaaa acagtggttt gtgtcattag
    10801 tggtggtaat aatgatatta atcgaatgaa agaaattgaa gaacgttcat tactatacga
    10861 agaaatgaag cattacttta tcttaaattt ccctcaacgt ccaggtgcat tgagagaatt
    10921 tgtaaatgac gtattaggac ctcaagacga tattactaaa tttgaatact taaaaaaatc
    10981 ttctcaaaat acaggtactg tcattattgg tattcaactt aaagatcatg atgatttaat
    11041 acaactcaaa caacgtgtaa atcatttcga tccttccaat atttatatta atgaaaataa
    11101 gatgttatat tcattgttaa tttaacacat agtaagaaaa acagtcataa attgatttct
    11161 aattgaaatc atcttatgac tgctttttat tatactttac atttctcatt tcgtcagatt
    11221 caaacgtttt cacttcgcca agccatcttt ctttgtgttt gcttttattt tgacgtttta
    11281 gacataaaaa aagagacctt gcggtctcaa tgcggctcat cgcatccact ttttgcctgg
    11341 caacgttcta ctctagcgga acgtaagttc gactaccatc gacgctaagg agcttaactt
    11401 ctgtgttcgg catgggaaca ggtgtgacct ccttgctata gtcaccagac atatgaatgt
    11461 aa
//
LOCUS       DALAGF010000086        11354 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.086, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000086 DALAGF010000000
VERSION     DALAGF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11354
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.086"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            23..976
                     /locus_tag="O2692_002086"
     CDS             23..976
                     /locus_tag="O2692_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299706.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(1139..1342)
                     /locus_tag="O2692_002087"
     CDS             complement(1139..1342)
                     /locus_tag="O2692_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCZ4299707.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            1641..2129
                     /locus_tag="O2692_002088"
     CDS             1641..2129
                     /locus_tag="O2692_002088"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCZ4299708.1"
                     /translation="MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            2122..3057
                     /locus_tag="O2692_002089"
     CDS             2122..3057
                     /locus_tag="O2692_002089"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCZ4299709.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPNLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            3054..4361
                     /gene="rsmB"
                     /locus_tag="O2692_002090"
     CDS             3054..4361
                     /gene="rsmB"
                     /locus_tag="O2692_002090"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCZ4299710.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            4364..5458
                     /gene="rlmN"
                     /locus_tag="O2692_002091"
     CDS             4364..5458
                     /gene="rlmN"
                     /locus_tag="O2692_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCZ4299711.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            5465..6208
                     /locus_tag="O2692_002092"
     CDS             5465..6208
                     /locus_tag="O2692_002092"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HCZ4299712.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            6205..8199
                     /gene="pknB"
                     /locus_tag="O2692_002093"
     CDS             6205..8199
                     /gene="pknB"
                     /locus_tag="O2692_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HCZ4299713.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            8427..9302
                     /gene="rsgA"
                     /locus_tag="O2692_002094"
     CDS             8427..9302
                     /gene="rsgA"
                     /locus_tag="O2692_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCZ4299714.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERENELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARILVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            9303..9947
                     /locus_tag="O2692_002095"
     CDS             9303..9947
                     /locus_tag="O2692_002095"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCZ4299715.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            9954..10595
                     /locus_tag="O2692_002096"
     CDS             9954..10595
                     /locus_tag="O2692_002096"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCZ4299716.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(10976..11164)
                     /locus_tag="O2692_002097"
     CDS             complement(10976..11164)
                     /locus_tag="O2692_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCZ4299717.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
BASE COUNT         4226 a         1560 c         2121 g         3447 t
ORIGIN      
        1 ataacttaag aggagaaagt ttatgaaaaa gacactggga tgtttacttt taattatgct
       61 tttagtcgta gcaggttgtt cttttggtgg gaatcataaa ttatcatcaa agaaatcaga
      121 agaatcaaaa caagaaactg taaaaaaaga atcggaagaa gagaaagatc cagatttaga
      181 gaaatatgaa gaaatagaga agaaaatgaa aggaattaaa gatgcgccat ctcttgataa
      241 gttggatcca ttaatgacag aaaagtcgtt tacgaatagt aaagggattc aaggatggaa
      301 agattacaaa gaattaatgg gtaaagtgga acttgcagat tatagattta ctaaagattc
      361 aaaaggatct tcaataaaag atgttgatgc attctttaaa ggtaagaaag gtataaaaag
      421 gaaagtgatt gaaacacacg atgatgtaaa acaagttgat tattggtatg tagatccaga
      481 tggaaagaaa attggcaatt caaacacacc tgttttttac gcagaaatta tgacaaaata
      541 taaagatgga aagttagttt atgcatcagt cgaaccagga tcttacgtaa tacataaaga
      601 tgatgcaatt aaatatgacg attattctaa gttaaaaaaa ttaagtcagc taactaaact
      661 tgatcatcca aaaccagttc catatagcgt agctcaaatc aaatctttcg gagtaccttt
      721 aacaagcgtt tcatttatga cacatggatc aaaggatact aaagatgaag tgttgccggc
      781 attggcctat ttcacttttt caccaaaaaa ttatgaagac aagtctaatc cagatccaaa
      841 agttttaaat ttagtacata tggatttctt aaatgcatct agtgattttg gtaacgcaca
      901 ttttgttgtt ttaagtaaat atattaaaga gtatgaatca aactatgaaa cagcgtcaga
      961 tgattcttta aaatagtatt tactgtgtga aaaataaata gtgtactaca ttaaataatc
     1021 gcaataataa tcccgataaa caatcagcat tactgcttat cacatagagt tcgtaataac
     1081 tataactcta tgattcgcaa ataataaatg attgtcatcg ggatttattt ttatcaattt
     1141 ataaagtgac attaccttgt tcatcagcag gtttgaaaac agtaatcact gcactaataa
     1201 ttgctaaaat gtgtgggata cctgtccaac agaatattaa gtgtagaata ccttgcatat
     1261 tcttgccggc ataaaattta tgaataccaa aactacctaa gaacaatgct aataaaatat
     1321 aaataacttt gtttacttgc atttctttcc ctccagttga attgcttata atgacattag
     1381 cttctctttt tattataccc acttttagtt caaacattct agtttaagca ttcccaatca
     1441 tctaaatttc agttattcaa tccttacaat aaatttagga ttacatttca gttgcattgt
     1501 attattttac gtgtgaaata tacgtaatga atcacatgac aatcttcaaa ttgaaaaata
     1561 tacattctat gatgtaaggt cgcattttta atatatttac gttataatag tttgatgaat
     1621 tttattgagg agtaagagtt atggcgatta aaaagttagt accagcatcg catcctattt
     1681 taacgaaaaa agcgcaagca gttaaaacat ttgatgattc gttaaaaaga ttattacaag
     1741 atttagaaga tacaatgtat gcacaagaag ctgctggctt atgtgcacct caaattaatc
     1801 agtcattgca agtggcaatt attgatatgg aaatggaagg attattacaa cttgttaatc
     1861 cgaaaattat tagtcaatca aatgaaacaa taacagactt agaaggttca attacattgc
     1921 cagatgttta cggcgaagtg acaagaagta aaatgatagt tgtcgaaagt tatgacgtca
     1981 atgggaacaa agttgaacta actgcacatg aagatgtagc aagaatgatt ttgcatatta
     2041 tagatcaaat gaacggtatc ccttttacag aacgtgcgga ccgtatttta acagataaag
     2101 aagtggaggc atattttata aatgactaaa ataatattta tgggtacacc agacttttca
     2161 acaactgttt tagaaatgct tattgcagaa catgatgtca ttgcagtcgt aacgcaacca
     2221 gatcgacctg ttggacgtaa acgtgttatg acaccaccac cagttaaaaa agttgcaatg
     2281 aaatatgatt tacctgtata tcaacctgaa aaattaagtg gatcagaaga attagaacaa
     2341 ttgcttcaat tagatgtaga tttaattgta actgctgctt ttggacaatt attacctgaa
     2401 tcattgttgg cattaccaaa tcttggggca attaatgtac atgcatcatt gttaccgaag
     2461 tatagaggtg gtgcaccaat tcatcaggca attatcgatg gtgaacaaga aaccggcata
     2521 acaattatgt atatggttaa aaaattagat gcgggtaata ttatttcgca acaagcaatt
     2581 aaaatagaag aaaatgataa tgtcggtacg atgcatgata aattaagtgt attaggggca
     2641 gatttattaa aagaaacttt accatctatt atagagggca caaatgaaag cgtacctcaa
     2701 gatgatacgc aagcaacatt tgcttccaat attcgacgcg aagatgagcg aattagctgg
     2761 aataaaccag gaagacaagt gtttaatcaa attcgtggat tatcaccatg gccagttgct
     2821 tatacaacta tggatgacac taacttgaaa atatacgatg ctgaactcgt tgagactaat
     2881 aagataaacg agcctggaac cattatagaa acgactaaaa aagccattat tgttgctaca
     2941 aatgataatg aagctgttgc aattaaagat atgcaattag ctgggaaaaa gagaatgtta
     3001 gctgccaatt atttaagtgg tgcgcaaaac acactagtag ggaagaaact tatatgatag
     3061 aaaacgtgag aagtcttgct tttgacacga ttcaagatat attaaatgaa ggtgcgtata
     3121 gtaacttgcg tatcaatgaa gtgttgtcag aaaatgaatt aaatgcaatg gataaggctt
     3181 tatttacaga aattgtctac ggaaccgtta aaagaaaata tacgttagat ttttatttaa
     3241 agccttttgt gaaaacaaag attaaggcat gggttaggca attattatgg atgagtattt
     3301 atcaatatgt ttatttagat aaagttccaa atcatgccat tattaatgaa gcagttgaaa
     3361 tagcaaaaga acgcggtggc tatcataatg gtaatgtcgt aaatggtatt ttacgtacaa
     3421 tgatgcgtag tgacttacct gattttaatg aaattgcaga tcctaaaaaa agaatggcaa
     3481 tcgagtatag tatgccgaag tggattatag atcattgggc aacacattat ggtctcgaag
     3541 aaactgaaac aattttacag tcatttttag aaacgacatc aacaactgtg cgtgccaacc
     3601 tgacgcgagc atcattagat gatattattg aaaagttgca agacgaaggt tatgacgttg
     3661 aaaaagatca tgacttacct tattgtctcc atataggagg acaaccaatt attcattctc
     3721 gttcatttaa agatggattc gtttcaattc aagataaaag ctcaatgttt gttgcacaca
     3781 ttatgaatgt agaccgacat gatcacgtat tagatgcatg tagtgcacct ggcggtaaag
     3841 cttgtcacat tgctgaagtt ttaatgccag aagggcaagt tgacgcttca gatatacatg
     3901 atcacaaaat agacttaatt aattttaata taaaaaaatt acgattaaca aatattaaag
     3961 cttttcaaca tgatgcgaca aaaccttatg ataaaacata cgataagata cttgttgatg
     4021 caccatgtag cggattaggt gtaatgagac ataagccgga gattaagtat actcaaagca
     4081 aacaacatat tgagtcacta gttgaattac agcttgaaat attggaaaat gtaaaaaaca
     4141 atgtaaaaat aggtggcgaa atcatctatt caacatgtac aattgagcaa ctagaaaatg
     4201 aaaacgtgat ttatacgttt ttgaaaaata ataaaaactt cgaatttgaa ccgtttcaac
     4261 atccgataac tggagagttg gtcaaaacgt tacaaatcat gccgcaagac tttaattcag
     4321 atggattctt tatcactaag ataaaaagaa aggacaatta ggaatgataa ctgctgaaaa
     4381 gaaaaagaag aataaatttc ttccaaattt tgacaagcaa tcaatatatt cattgcgatt
     4441 tgacgaaatg caaaactggc tcgttgaaca agggcaacaa aaatttcgag cgaaacagat
     4501 ttttgaatgg ttatatcaaa aaagagtaga ttcgattgat gaaatgacga acttatcgaa
     4561 agacttacga cagcttttaa aagataactt tactgttaca actttaacaa ctgtagtaaa
     4621 acaagaaagt aaagacggta caattaaatt cttatttgaa ttacaagatg gctatacaat
     4681 tgaaactgtt ttaatgagac atgattatgg aaattcagta tgtgtaacga cacaagtagg
     4741 ttgtcgcatc ggatgtacgt tttgtgcttc tacacttggc ggcttaaaaa gaaaccttga
     4801 agctggcgaa attgtttcac aagttttaac agttcaaaaa gcccttgatg ctacagaaga
     4861 gcgcgtatct caaattgtca taatgggtat cggtgaacca tttgaaaatt atgatgaaat
     4921 gatggacttt ttaagaatcg tcaatgatga taacagttta aatattggtg cacgtcacat
     4981 tacagtatca acatcaggta tcattcctag aatatatgac tttgcggatg aagatattca
     5041 aattaatttt gctgtaagct tacacgccgc aaaagatgaa gtgcgatcac gcttgatgcc
     5101 aattaaccgt gcatataatg ttgagaagtt aatcgaagca attcaatatt atcaagaaaa
     5161 aacaaatcgt cgtgttactt ttgaatatgg tctgtttggt ggtgtgaatg accaactaga
     5221 acatgcaaga gaattagcac atttaataaa aggcttaaac tgccatgtta acttaattcc
     5281 tgtcaaccat gttccagaaa gaaattatgt gaaaacggct aaaaatgata tctttaaatt
     5341 tgaaaaagaa ttaaagagac taggaattaa tgccacaata cgtcgtgaac aaggttcgga
     5401 tattgacgca gcttgtggtc aattaagagc aaaggaacga caagtagaaa cgaggtaaag
     5461 acaaatgcta gaggcacaat tttttactga tactggacaa catagagata agaatgaaga
     5521 tgcgggtggt attttttata atcaaactaa tcaacaactt ttagttctgt gtgatggtat
     5581 gggtggccat aaagcaggag aagttgcaag taaatttgtt acagatgagt tgaaatcccg
     5641 ttttgaagcg gaaaatctta tagaacaaca tcaagctgaa aattggttgc gtaataatat
     5701 aaaagatata aattttcagt tatatcacta tgcacaagaa aatgcagaat ataaaggtat
     5761 gggtacaaca tgtgtttgtg cacttgtttt tgaaaaatca gttgtgatag caaatgtcgg
     5821 tgattctaga gcctatgtta ttaatagtcg acaaattgaa caaattacta gtgatcactc
     5881 atttgttaat catcttgttt taacgggtca aattacgccg gaagaagcat ttacacatcc
     5941 acaacgtaat attattacga aggtgatggg cacagataaa cgtgtgagtc cagatttgtt
     6001 tattaagcga ttaaattttt atgattattt attattaaat tcagatggat taactgatta
     6061 tgttaaagac aatgaaatta agcgtttgtt agtaaaagaa ggtacaatag aagatcatgg
     6121 tgatcaatta atgcaattgg cattagataa ccattcgaaa gataacgtta ctttcatact
     6181 cgcggctatt gaaggtgata aagtatgata ggtaaaataa taaatgaacg atataaaatt
     6241 gtagataagc ttggcggcgg tggcatgagt accgtttatc ttgctgaaga tacgatactt
     6301 aacattaaag ttgcaattaa ggcgattttt ataccaccta gagaaaaaga agaaacatta
     6361 aaacgttttg aacgagaagt acataactca tcacagctat cacatcaaaa tatagtaagt
     6421 atgatcgatg ttgatgaaga agatgactgt tactacttag taatggaata tatcgaaggt
     6481 ccgactttgt ctgagtatat tgaaagtcat gggccattaa gtgttgacac agcgattaat
     6541 tttacgaatc aaatattgga tggcattaaa catgcgcatg atatgcgtat tgtacataga
     6601 gatattaagc cacaaaatat attaattgac agcaataaaa cgttgaaaat atttgatttt
     6661 ggaattgcta aagctttaag tgagacgtct ttaactcaga ctaatcatgt gttaggtact
     6721 gtgcagtact tttcgccaga acaagcaaaa ggtgaggcaa cggatgaatg tacagatatt
     6781 tattctatag gtattgtgtt atatgaaatg cttgttggtg aaccaccctt taatggagaa
     6841 actgcagtta gcattgcgat taaacatatt caggattctg tgccaaatgt gacaacagat
     6901 gtacgtaagg atattccgca atctttaagt aatgtcattt tacgcgctac agaaaaagac
     6961 aaagcgaatc gttacaaaac aattcaagaa atgaaagatg atttgagtag tgttttacat
     7021 gaaaatcgag cgaatgaaga tgtctatgaa ctcgataaaa tgaaaacgat agcggtacct
     7081 ttgaaaaaag aagatctagc aaagcatatt agtgaacata agtcgaatca acctaaacgt
     7141 gaaacgacgc aagtacctat tgtaaatggg cctgctcatc atcagcaatt ccaaaagcca
     7201 gaaggtacgg tgtacgaacc aaaacctaaa aagaaatcaa cacgaaagat tgtgctctta
     7261 tcactaatct tttcgttgtt aatgattgca cttgtttctt ttgtggcaat ggcaatgttt
     7321 ggtaataaat acgaagagac acctgatgta atcgggaaat ctgtaaaaga agcagagcaa
     7381 atattcaata aaaacaacct gaaattgggt aaaatttcta gaagttatag tgataaatat
     7441 cctgaaaatg aaattattaa gacaactcct aatactggtg aacgtgttga acgtggtgac
     7501 agtgttgatg ttgttatatc aaagggccct gaaaaggtta aaatgccaaa tgtcattggt
     7561 ttacctaagg aggaagcctt gcagaaatta aaatcgttag gtcttaaaga tgttacgatt
     7621 gaaaaagtat ataataatca agcgccaaaa ggatacattg caaatcaaag tgtaaccgca
     7681 aatactgaaa tcgctattca tgattctaat attaaactat atgaatcttt aggcattaag
     7741 caagtttatg tagaagactt tgaacataaa tcctttagca aagctaaaaa agccttagaa
     7801 gaaaaagggt ttaaagttga aagtaaggaa gagtatagtg acgatattga tgagggtgat
     7861 gtgatttctc aatctcctaa aggaaaatca gtagatgagg ggtcaacgat ttcatttgtt
     7921 gtttctaaag gtaaaaaaag tgactcatca gatgtcaaaa cgacaactga atcggtagat
     7981 gtaccataca ctggtaaaaa tgataagtca caaaaagtta aagtttatat taaagataaa
     8041 gataatgacg gttcaactga aaaaggtagt ttcgatatta ctagtgatca acgtatagac
     8101 attcctttaa gaattgaaaa aggaaaaaca gcaagttata ttgttaaagt tgacggtaaa
     8161 actgtagctg aaaaagaagt cagctatgat gatgtataaa tataattgaa gtaaatgtac
     8221 cgaggtttct atttggaagt ctcggtattt ttatgttgag attgcggtag ttttaaaatg
     8281 cttcatgtca tcatatacag gtgagcattg tctatcaatt ctgtatcact ctcacaatat
     8341 ttatatcatt tgaaatgatc atttatatat taattttcca gtctttgata taataaatgc
     8401 agtaaaatta atcgagaggt gccattttga agacaggtcg aatagtgaaa tcaattagtg
     8461 gggtatatca agtagacgtt aatggcgaac gtttcaatac aaaaccacga ggattattta
     8521 gaaagaaaaa attttcaccg gtagttggtg atatagtgga atttgaagtt caaaacatta
     8581 acgaaggtta tattcatcaa gtgtttgagc gggaaaatga gttgaaaaga ccacctgtaa
     8641 gtaatataga tacactagta attgtaatga gtgctgtcga gccaaatttt tcaacgcaat
     8701 tattagatcg atttttagtt attgcacatt cgtatcagtt aaatgcgaga attttggtga
     8761 ctaaaaaaga taaaacacca attgaaaagc agttcgaaat aaatgagttg ttgaaaatat
     8821 atgaaaatat tggctatgag actgaattta ttggaaatga tgatgatcga aaaaaaattg
     8881 tagaagcttg gccagctgga cttatagtac ttagtggtca atcaggtgtc ggtaagtcca
     8941 ctttcttaaa tcattatcgt ccagaactta atcttgagac aaatgatata tcaaaatcat
     9001 taaatcgagg aaagcatact acaagacatg tcgaactatt cgaacgtcaa aacggttata
     9061 ttgcagacac acctggattc agtgctttag attttgatca tatagataaa gatgaaataa
     9121 aagattattt tcttgaatta aatcgatatg gtgaaacatg taagtttagg aattgtaatc
     9181 atatcaaaga acctaattgt aatgttaagc atcaattaga gatagggaat attgcgcaat
     9241 ttagatacga ccattattta caactattta atgaaatttc aaatagaaag gttagatatt
     9301 aaatgacaaa actatatcca tcattattat ctgttgattt tttggattta caacatgaat
     9361 taaaacgact tgaagaagca ggcgtcgacg gagttcattt tgatgttatg gatggtcaat
     9421 ttgtgcctaa tatatctatt ggtttaccaa tattagatgc agtaagaaaa ggcacaacat
     9481 tacctataga cgtacatttg atgattgaaa atccagaaaa gtatattgca tcatttgcag
     9541 aacatggtgc cgatatgatt tcaattcatg tcgaatcaac gcctcatatt catcgtgcta
     9601 ttcaaatgat taaacattta gataaaaaag ctggtgtagt aattaatcct ggtacaccaa
     9661 tatcacaaat tgaacctatt ttagacattg ttgattatgt actagtgatg acagttaacc
     9721 cagggtttgg tggtcaatca tttattgatc aatgcgtaga aaaaatagcg ggtcttaatg
     9781 ctattaaaat ggaacgtcaa ttaaactttg atattgaagt tgatggaggc gtaaataccg
     9841 atacagcgaa agtttgtgtt gaaaatggtg ctacaatgct agtaacaggt tcatttttct
     9901 ttaaacaaga ggattataaa aaagtcacac aagcattgaa aggttgagtg tatatgcata
     9961 taaatttatt atgttctgat cgacacttgc cgcaagatat ttgggccaaa agtaatgaag
    10021 gtaaatgggg cggcgttgat agaggtgctt tgattttatt gaagcatcaa attatccctt
    10081 ttttctcagt gggagacttt gattcagtca gtaaagaaga acgccaactt ctaacagaac
    10141 agttacaaat caaaccagtt caagctgaaa aagctgatac ggatttagct ttagcggttg
    10201 ataaagctgt tgcacttgga tttgatagta ttacaattta tggtgcaaca ggcggacgat
    10261 tagatcactt ttttggggca attcagttat tattgaaaaa agcatattat aaacatgatg
    10321 ttcatataga agttatcgat caacaaaata aaattgaatt attgcctaaa ggtcaacata
    10381 cagttgaaaa agataagagt tatccgtaca tttcatttat accgatgact gatgatgtag
    10441 aactttctct agcaggtttt aaatataatt tagctagaca aatgcttaat ataggttcta
    10501 ctttaactat ttcaaatgaa attgagtctt tgcaagcgaa agtaactgta catgatgggt
    10561 tgattttgca aattagaagt acagatttaa attaagtata ttcaaatttg tgaggatttt
    10621 taataaagtg catgagttat ttaataaagt gacgtttata gatagaattt ataacataat
    10681 aatagatgaa tcacgctgta attaatgaag attatggcgt gatttattat tgaaaggaaa
    10741 attgtaggtc ctagtacatt gatttatgtg ccaaaatcga tattgaaccc ataagtccaa
    10801 tttgacagag aaccattatt aaacgtatta accaaattat attgatagtt cgaatttcac
    10861 tatgaaccat tatgtattgt ttggtaacaa aaaaagaact aagaggttga gctcttagtt
    10921 cttaagtaaa tgtgtcctcc acataaggtc acatttttta tgtgcaatat atttattaaa
    10981 ctctagttac tttaccagat tttaaagcac gtgcagaaac ccaaactttt ttaggtttac
    11041 cgtcaactag gattctaact ttttgaaggt tagcgttcca tctacgttta gtagagttta
    11101 aagcgtgtga acgtctgtta ccagtcgaag ctttacgacc tgttacgaaa cattgtttac
    11161 ccatatgagt acctccttta ataaatataa atacacataa ctacttatat acttaattaa
    11221 gatagcatag tttcatttga aaaacaatga ataattttca cataaaagtc aaaaatactg
    11281 agttttgtga tataattgta gactgtgaag ttatgtagta tgatatttta gagaaataag
    11341 tgaatgaaac attt
//
LOCUS       DALAGF010000087        11318 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.087, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000087 DALAGF010000000
VERSION     DALAGF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11318
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.087"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            122..6682
                     /gene="sasC"
                     /locus_tag="O2692_002098"
     CDS             122..6682
                     /gene="sasC"
                     /locus_tag="O2692_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HCZ4299718.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDKDVANNRGLANSAQNTPNQSATTNQATNQALVNHNNGSIVNQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVSNANNNDVVSNNTALNVPTKTNENGSGGHLT
                     LKEIQEDVRHSSNKPELVAIAEPASNRPKKRSRRAAPADPNATPADPAAAAVGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNGIRPSTNRSVPTVNVVNNLPGFTLIN
                     GGKVGVFSHAMVRTSMFDSGDNKNYQAQGNVIALGRIHGTDTNDHGDFNGIEKALTVN
                     PNSELIFEFNTMTTKNGQGATNVIIKNADTNDTIAEKTVEGGPTLRLFKVPDNVRNLK
                     IQFVPKNDAITDARGIYQLKDGYKYYSFVDSIGLHSGSHVFVERRTMDPTATNNKEFT
                     VTTSLKNNGNSGASLDTNDFVYQVQLPEGVEYVNNSLTKDFPSNNSGVDVNDMNVTYD
                     AANRVITIKSTGGGTANSPARLMPDKILDLRYKLRVNNVPTPRTVTFNETLTYKTYTQ
                     DFINSAAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNGLKRRAQT
                     ILDENRNNVPLNKRVSQAYIDSLTNQMQHTLIRSVDAENAVNKKVDQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDVTEDEIQDALNQLATDETDAIDNVTNATTNADVETAKNNG
                     INTIGAVVPQVTHKKAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDAT
                     QEERQAAIDKVNAAVTAANTNILNANTNADVEQVKTNAIQGIQAITPATKVKTDAKNA
                     IDKSAETQHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDTRNVVNDKAREAITNINATTGATREEKQEAINRVNTLKNRALT
                     DIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQI
                     NQHYNAKLAEINATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAINQ
                     INQNQTNDQVDTTTNQAVNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDN
                     EKEVASQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKIN
                     QKANELRAKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDS
                     IALVTPDHIVRAAARDAVKQQYEAKKREIEQAEHATDEEKQVALNQLANNEKRALQNI
                     DQAIANNDVKRVETNGIATLKGVQPHIVIKPEAQQAIKASAENQVESIKDTPHATVDE
                     LDEANQLISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQIN
                     HADKTAQVNQDSINAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQ
                     NIAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVINRKASAREQLTTL
                     FNDKKQAIEANIQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIH
                     DLDVHPIKKPDAEKTINDDLARVTALVQNYRKVSNRNKADALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKV
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEATKVSPKEIQPAPKV
                     CTPIKKEETHESRKVEKELPNTGSEGMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(6799..8067)
                     /locus_tag="O2692_002099"
     CDS             complement(6799..8067)
                     /locus_tag="O2692_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCZ4299719.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKHISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
     gene            8474..10135
                     /locus_tag="O2692_002100"
     CDS             8474..10135
                     /locus_tag="O2692_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCZ4299720.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            10132..10827
                     /locus_tag="O2692_002101"
     CDS             10132..10827
                     /locus_tag="O2692_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCZ4299721.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            10844..11266
                     /locus_tag="O2692_002102"
     CDS             10844..11266
                     /locus_tag="O2692_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCZ4299722.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFKEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
BASE COUNT         4358 a         1805 c         2010 g         3145 t
ORIGIN      
        1 ttgagtgaac ttaaaatatt gtacaaaata atatagctat aaatataata tgagggagcg
       61 tatattttta gcataattct taacaacaca gcagagaaca gacaaccagg aggaaaatga
      121 aatgaatttg ttaaagaaaa ataaatatag tattaggaag tataaagtag gcatattctc
      181 tactttaatc ggaacagttt tattactttc aaacccaaat ggtgcacaag ccttaactac
      241 ggataataat gtacaaagcg atactaatca agcaacacct gtaaattcac aagataaaga
      301 tgttgctaat aatagaggtt tagcaaatag tgcgcagaat acacctaatc aatctgcaac
      361 aaccaatcaa gcaacgaatc aagcattggt taatcataat aatggtagta tagtaaatca
      421 agctacgcca acatcagtgc aatcaagtac gccttcagca caaaacaata atcatacaga
      481 tggcaataca acagcaactg agacagtgtc aaacgctaat aataatgatg tagtgtcgaa
      541 taataccgca ttaaatgtac caactaaaac aaatgaaaat ggttcaggag gacatctaac
      601 tttaaaggaa attcaagaag atgttcgtca ttcttcaaat aaaccagagc tagttgcaat
      661 tgctgaacca gcatctaata gaccgaaaaa gagaagtaga cgtgcggcac cggcagatcc
      721 taatgcaact ccagcagatc cagcggctgc agcggtagga aacggtggtg caccagttgc
      781 aattacagcg ccatatacgc caacaactga tcctaatgcc aataatgcag gacaaaatgc
      841 acctaacgaa gtgctgtcat ttgatgacaa tggtattaga ccaagtacca accgttctgt
      901 gccaacagta aacgttgtta ataacttgcc gggcttcaca ctaatcaatg gtggcaaagt
      961 aggggtgttt agtcatgcaa tggtaagaac gagcatgttt gattcaggag ataataagaa
     1021 ctatcaagca caaggaaatg taattgcatt aggtcgtata catggaactg atacgaatga
     1081 ccatggcgat tttaatggta tcgagaaagc attaacagta aatccgaatt ctgaattaat
     1141 ctttgaattt aatacaatga ctactaaaaa cggtcaaggc gcaacaaatg ttattatcaa
     1201 aaatgctgat actaatgata cgattgctga aaagactgtt gaaggcggtc caactttgcg
     1261 tttatttaaa gtacctgata atgtgagaaa tctcaaaatt caatttgtac ctaaaaatga
     1321 cgcaataaca gatgcgcgtg gcatttatca actaaaagat ggttacaaat actatagctt
     1381 tgttgactct atcggacttc attctgggtc acatgttttt gttgaaagac gaacaatgga
     1441 tccaacagca acaaataata aagagtttac tgtaacaaca tcattaaaga ataatggtaa
     1501 ttctggtgct tctctagata caaatgactt tgtatatcaa gttcaattac ctgaaggtgt
     1561 tgaatatgtg aacaattcat tgactaaaga ttttccaagt aacaattcag gcgttgatgt
     1621 taatgatatg aatgttacat atgatgcagc aaatcgtgtg ataacaatta aaagtactgg
     1681 aggaggtaca gcaaactctc cggcacgact tatgcctgat aaaatactcg atttaagata
     1741 taaattacgt gtaaataatg tgccgacacc aagaacagta acatttaacg agacattaac
     1801 gtataaaaca tatacacaag atttcattaa ttcagctgca gaaagtcata ctgtaagtac
     1861 aaatccatat actatcgata tcatcatgaa taaagatgca ttacaagccg aagttgacag
     1921 acgtattcaa caagctgatt atacatttgc gtcattagat atctttaatg gtctgaaacg
     1981 acgcgcacaa acgattttag atgaaaatcg taacaatgta ccattaaata aaagagtttc
     2041 tcaagcatat attgattcat taactaatca aatgcaacat acgttaattc gaagtgttga
     2101 tgctgaaaat gcagttaata aaaaagttga ccaaatggaa gatttagtta atcaaaatga
     2161 tgaattgaca gatgaagaaa aacaagcagc aatacaagtt atcgaggaac ataaaaatga
     2221 aataattggt aatattggtg accaaacgac tgatgatggc gttactagaa tcaaagatca
     2281 aggtatacag accttaagtg gggatactgc aacaccggtt gttaaaccaa atgctaaaaa
     2341 agcaatacgt gataaagcaa cgaaacaaag ggaaattatc aatgcaacac cagatgttac
     2401 tgaagacgag attcaagatg cactaaatca attagctacg gatgaaacag atgctattga
     2461 taatgttacg aatgctacta caaatgctga cgttgaaaca gctaaaaata atggcatcaa
     2521 tactattgga gcagttgttc ctcaagtaac tcataaaaaa gctgcaagag atgcaattaa
     2581 ccaagcaaca gcaacgaaaa gacaacaaat aaatagtaat agagaagcaa ctcaggaaga
     2641 gaaaaatgca gcattgaacg aattaactca agcaaccaac catgctttag aacaaatcaa
     2701 tcaagcaaca acaaatgctg atgttgataa cgccaaagga gatggtctaa atgccattaa
     2761 tccaattgct cctgtaactg ttgttaagca agctgcaagg gatgccgtat cacatgatgc
     2821 acaacaacat atcgcagaga tcaatgctaa tcctgatgcg actcaagaag aaagacaagc
     2881 agcaattgac aaagtgaatg ctgctgtaac tgcagcaaac acaaacattt taaacgctaa
     2941 taccaatgct gatgttgaac aagtaaagac aaatgcgatt caaggaatac aagcaattac
     3001 accagctaca aaagtaaaaa cagatgcaaa aaatgccatc gataaaagtg cggaaacgca
     3061 acataatacg atatttaata ataatgatgc gacgctcgaa gaacaacaag cagcacaaca
     3121 attacttgat caagctgtag ccacagcgaa gcaaaatatt aatgcagcag atacgaatca
     3181 agaagttgca caagcaaaag atcagggcac acaaaatata gtagtgattc aaccggcaac
     3241 acaagttaaa acggatactc gcaatgttgt aaatgataaa gcgcgagagg cgataacaaa
     3301 tatcaatgct acaactggcg cgactcgaga agagaaacaa gaagcgataa atcgtgtcaa
     3361 tacacttaaa aatagagcat taactgatat tggtgtgacg tctactactg cgatggtcaa
     3421 tagtattaga gacgatgcag tcaatcaaat cggcgcagtt caaccgcatg taacgaagaa
     3481 acaaactgct acaggtgtat taaatgattt agcaactgct aaaaagcaag aaattaatca
     3541 aaacacaaat gcaacaactg aagaaaagca agtggcttta aatcaagtgg atcaagagtt
     3601 agcaacggca attaataata taaatcaagc tgatacaaat gcggaagtag atcaagcgca
     3661 acaattaggt acaaaagcaa ttaatgcgat tcagccaaat attgttaaaa aacctgcagc
     3721 attagcacaa atcaatcagc attataatgc taaattagct gaaatcaatg ctacaccaga
     3781 tgcaacgaat gatgagaaaa atgctgcgat caatacttta aatcaagaca gacaacaagc
     3841 tattgaaagt attaaacaag ctaacacaaa tgcagaagta gaccaagctg cgacagtagc
     3901 agagaataat atcgatgctg ttcaagttga tgtagtaaaa aaacaagcag cgcgagataa
     3961 aatcactgct gaagtggcga agcgtattga agcggttaaa caaacaccta atgcaactga
     4021 cgaagaaaag caggctgctg ttaatcaaat caatcaactt aaagatcaag caattaatca
     4081 aattaatcaa aaccaaacaa atgatcaggt agacacaact acaaatcaag cggtaaatgc
     4141 tatagataat gttgaagctg aagtagtaat taaaccaaag gcaattgcag atattgaaaa
     4201 agctgttaaa gaaaagcaac agcaaattga taatagtctt gattcaacag ataatgagaa
     4261 agaagttgct tcacaagcat tagctaaaga aaaagaaaaa gcacttgcag ctattgacca
     4321 agctcaaacg aatagtcagg tgaatcaagc agcaacaaat ggtgtatcag cgattaaaat
     4381 tattcaacct gaaacaaaag ttaaaccagc tgcacgtgaa aaaatcaatc aaaaagcgaa
     4441 tgaattacgt gctaagatta atcaggataa agaagcaaca gcagaagaaa gacaagtagc
     4501 actagataaa atcaatgaat ttgtaaatca agccatgaca gatattacga ataatagaac
     4561 aaatcaacaa gttgatgata caacaagtca agcgcttgat agcattgctt tagtgacgcc
     4621 tgaccatatt gttagagcag ctgctagaga tgcagttaag caacaatatg aagctaaaaa
     4681 gcgcgaaatt gagcaagcgg aacatgcgac tgatgaagaa aaacaagttg ctttaaatca
     4741 attagcgaat aatgaaaaac gtgcattaca aaacatcgat caagcaatag cgaataatga
     4801 tgtgaaacgt gttgaaacaa atggcattgc tacactaaaa ggtgtacaac ctcatattgt
     4861 aattaagcct gaagcacaac aagcaataaa agcaagtgca gaaaatcaag tagaatcaat
     4921 aaaagataca ccacatgcaa cagttgatga attagatgaa gcgaatcaat taattagcga
     4981 cacactcaaa caagcgcaac aagaaataga aaatacaaat caagatgctg ctgttactga
     5041 tgttagaaat caaacaatca aggcaataga gcaaataaaa cctaaagtaa gacgtaaacg
     5101 agctgcgctt gatagcattg aagaaaataa taaaaatcaa ctcgatgcaa tccgaaatac
     5161 gttggatact actcaagatg aaagagatgt tgctattgat actttaaata aaattgtaaa
     5221 tacaattaaa aatgacattg cacaaaacaa aacgaatgca gaagtggatc gaactgagac
     5281 tgatggcaac gacaacatca aagtgatttt acctaaagtt caagttaaac cagcagcgcg
     5341 tcaatctgtt ggtgtaaaag ccgaagctca aaatgcacta atcgatcaaa gcgatttatc
     5401 aactgaagaa gaaagactag ctgctaaaca tttagtagaa caagcactta atcaggctat
     5461 tgatcagatc aatcatgcag ataagactgc ccaagttaat caagatagta taaatgctca
     5521 aaatattatt tcaaaaatta aaccagcgac aacagttaaa gcaacagcat tacaacaaat
     5581 tcaaaatatc gctacaaata aaattaattt aattaaagca aataacgaag cgacagatga
     5641 agaacaaaat attgcaatag cacaagttga aaaagagtta attaaagcta aacaacaaat
     5701 tgctagtgca gtgactaatg cagatgtggc atatttattg catgatgaga aaaacgaaat
     5761 tcgtgaaatc gaacctgtta ttaacagaaa ggcgtctgct cgagaacaat tgacaacatt
     5821 attcaacgat aaaaaacaag caattgaagc gaatattcaa gcaacggtag aagaaagaaa
     5881 tagtatatta gcacagttac aaaatattta tgacactgct attggacaaa ttgatcaaga
     5941 tcgtagcaat gcacaagttg ataaaacagc atcattaaat ctacaaacaa tacatgattt
     6001 agatgtacat cctattaaaa agccagatgc tgaaaaaacg attaatgatg atcttgcacg
     6061 cgtcactgct ttagtgcaaa attatcgaaa agtaagtaat cgtaataagg ctgatgcatt
     6121 aaaagctata actgctttaa aattacaaat ggatgaagaa ttaaaaacag cacgcactaa
     6181 tgctgatgtt gatgcagttt taaaacgatt taatgttgca ttaagcgata tagaagcagt
     6241 aattactgaa aaagaaaata gcttactgcg aattgataac attgctcaac aaacatatgc
     6301 gaaattcaaa gcgatcgcaa caccagaaca attagctaaa gtaaaagtat taattgatca
     6361 atatgttgca gatggcaata gaatgattga tgaagatgcg acattaaatg acatcaaaca
     6421 acacacgcaa ttcattgttg atgaaatttt agcaattaaa ttaccagctg aagcgacgaa
     6481 agtatcacca aaagaaattc agccagctcc aaaagtttgt acgcctatta aaaaagaaga
     6541 gacacatgaa tcgcgcaaag ttgaaaaaga acttccaaat acaggttctg aaggaatgga
     6601 tttaccattg aaagaatttg cactgattac aggtgcggct ttgttagcta gaagacgtac
     6661 taaaaacgaa aaagaatcat aattaacgaa atgaccttaa tataacgaca ctttttagtg
     6721 actcagttat attaaggtca tttttatttg aatgaaaaag ttgatctact aaagagaatc
     6781 gaaccaaatt ttatactatt attccattga tgcatgtgag taatgtccgg catataatcc
     6841 agcgacatgt cctgttacga gtgcacttgt aatattataa ccaccagtat aaccatgtat
     6901 atctaatact tcaccacata aaaataatcc cggaactaat ttagacatca ttgttttagg
     6961 ttgaatttct ttaagtgaca caccaccacc tgtgacaaat gccttatcta taggtaatgt
     7021 cccgttcacc ttaaatacaa accctttaaa catatttact aagtcgttca attgttgatt
     7081 tgataagtga tgtgatgtgg tattttcatc gattcctgct tgttccagca tgaacagtaa
     7141 gtagcgctct tcaattagac catgcaaact gtttttaatg attttatctg gtgtgtccga
     7201 taataatgat gtgatgtgtt gttttaattg ttcatggttt aattcaggaa atgcatcgat
     7261 tgccatagaa atgtgctgtg tcttttgatt tttttgttct ttataaacaa actgactaca
     7321 tcttaatgca gctggaccac tgataccaaa atgagtaaat aacatatcca tttgatgact
     7381 gatgtgtttt ttaccatttt tcttaagtac tgacaattca acatctttta aacttaaacc
     7441 ttttagacga ttggatttga tgaaaggttc agctgatgta attggaactt cggtcgggaa
     7501 taactcagta atggtatgac ctaaatcttg tgcgaactta taaccatcac cagttgaacc
     7561 agtttgaggg acacttgtac cacctgtagc aatcactagc gaatggcttt cataactatt
     7621 attttgagta tgtacagtga aagtttggtc tgtattaact tcgattctac taacagcttc
     7681 ttcttcttta atcgttacat gttggcgttc gatagttgtc actaatgtat caaccacgtc
     7741 ttgtgctttg ttggaaactg gaaacatacg cccgtgatct tcttctttta atttaacacc
     7801 cctagactca aaaaaatcta tgatggattc attatcaaaa attgaaaagg gactatataa
     7861 aaatttccca tttccaggaa tgttcttaat aatttcagca tatggtaatc gattagttac
     7921 gttacatcta ccgccaccag atattttgag tttacgacct agacctttct ttttttcaat
     7981 gagtaacaca ctgctacttt gttcgcttgc agctactgcc gccattaagc cgctaggtcc
     8041 gcctccgata ataattgttt gatacatata cgcttcctcc aaatttgtta ctggacattc
     8101 aattgctatt ttatagtact tttttaaact ttgctatatc tcttgtgtta tgagatgaca
     8161 attatgttga atatatttat aatgtgaact tcataaaaaa tgaagatgta tgtgtatgat
     8221 aagaatgatt tttctactgt tatgcgatgt tcatctcaaa tagtagtata atgtttatat
     8281 gaaatttcgt aatattctaa tcaaaatcta gtattcaatg acaatgtgtt gataattttg
     8341 ttatgataat tcattgaata tacgacaatg aaataaagtt ttgtgatata ttcataataa
     8401 tgaaaaatga ttttacagag aaacagcttt tagtttacaa aactttaata aacatgagat
     8461 agggagattc gtaatgagtg aaagtaaaga aatggtgcgt ggaacctttt taattaccat
     8521 tagtatatta attaccaagg tattgggcgt actttttatc attccattca actatttaat
     8581 tggtggacaa gaaaatatgg cgccgttcac atacgcttat gcaccatata atattgcaat
     8641 tgcagttgcg acagcaggtg taccattagc agcttcgaaa tatgttgcga aatataatgc
     8701 tattggggca tataaagtaa gtcagaaatt ttataaatcg agttttattg taatgagtat
     8761 taccggtgtt ttaggtttct tggtactgta tttcttagca ccttatattt ctgaattaac
     8821 attagctaga aacattcatg ataaaaacgg ttggtcagtc gatgatatta cttggattat
     8881 aagaatcatt agtatggttg ttatctttat tcctgtatta gcgacatgga gagggatatt
     8941 ccaaggttat aaatctatgg ggccaacagc agtgtcagaa gtaactgagc aaattgcacg
     9001 cgtgattttc atattaatcg gaagttattt agtactaaat gtctttgatg gctctatttt
     9061 attagccaat ggtattgcta cttttgcagc agcagttggt gcgattattg gtatattcac
     9121 actctggtat tactggagaa agcgtaagca taatattgat cgtatggtcg aatctgatta
     9181 cacagatata gatgtgtcat atggcaagat gtataaagaa attattgctt atagtatacc
     9241 gttcgttatt gtaagtttga attatccatt atttaactta gtagatcaat ttacacataa
     9301 cggtgcttta tctttagttg gtataccttc acagttacaa gatatattct ttaatatgct
     9361 aaatatgtca actaataaaa ttgtaatgat tccgacatca ttaagtgctg gttttgcagt
     9421 gagtttaatt ccttatatta caaaaacatt tgcagaaggt cgcttacatg aaatgcatca
     9481 tcaaattaga acatctattg gtgtattaat gtttataact gttccagcta gtattggaat
     9541 tatggccttg gcacaaccat tattcacagt tttctatggt tacgatccaa ttgttttagg
     9601 tcatgaccct aaccatgatg gtagtcgctt attattctat tatgcgcctg tagcaatact
     9661 aatttcacta ttaagtgtaa cggcatcaat gttacaaggt atagataaac agaaattaac
     9721 agtttatgtt attttagctt ctgtagttat caagttagcg ttaaactatc cattaattat
     9781 gttattccat acacctggtg caattttaag tactagtatc gcattattat ttgcaattgg
     9841 atgtaatttc tatattctta aaaaatatgc gaagtttaaa ttcagctata gttggattca
     9901 ttttgcaaaa atattcttgt attcatttat tatgatgcta ggtgtagaat tagtattttt
     9961 ccttgcaaat ctattcttag aacctactaa acttggttac ttaatcatta ttattttagg
    10021 tgttacagta ggtattctaa tttacggtac aatcacaatt aaaacgcgtc ttgcggacga
    10081 attcttaggt gaaattcctg aaaaattaag acgtagagtt aggtttttac gatgagaata
    10141 gataaatttt tagcaaatat gggcgtcggt acacgtaatg aggtcaaaca gttacttaaa
    10201 aaaggccttg taaatgtcaa cgaacaagta attaaatcac caaaaacaca tatagaacca
    10261 gaaaacgata agataacagt tcgtggagaa ttaatagaat acattgaaaa tgtctatatt
    10321 atgttaaata agccaaaagg ttatatatca gcaactgaag atcatcattc aaaaaccgtt
    10381 attgatttaa tacctgaata ccaacatcta aatatttttc cggttggtcg tcttgataaa
    10441 gatacagaag ggcttttatt gataacgaat gatggtgatt ttaatcatga attaatgagt
    10501 ccaaacaaac atgtttctaa aaaatatgaa gttatttcag caaatcctat cactgaagac
    10561 gacattcaag cttttaaaga gggtgtaaca ctgactgatg gcaaggttaa gcctgcaata
    10621 ttaacttata ttgataatca aacgtcacac gtaacaattt atgaaggaaa atatcatcaa
    10681 gttaaacgaa tgtttcatag cattcaaaat gaggtattac atttaagacg tatcaaaatt
    10741 gctgatttag aattggacag caatttagat tcaggagaat atcgtttatt aactgaaaat
    10801 gattttgaca aattaaatta taaataaaag ggagacgatt gatatggcaa aaggtaattt
    10861 atttaaagcg attttaggta taggtggcgc tgtagcagct gtacttgtta cacgtaaaga
    10921 tagtcgtgac aagctgaaag cagaatataa taaatataaa caagatcctc aaagctataa
    10981 agataatgct aaggataaag cgacgcaatt aggaacaatt gctaatgaaa caattaaaga
    11041 agtaaaaaca aatccgaaag aatatgctaa tagattaaaa aataatccaa aagcattttt
    11101 caaagaagaa aaatcaaaat ttaccgaata tgacaataag actgacgaaa gtattgaaaa
    11161 aggtaaattt gatgatgaag gtggcgcagc accaaataat aatttacgta tcgtcactga
    11221 agaagattta aaaaagaata aaaatgcatt atctgataaa gaataagatg tgagtaggac
    11281 agaaatgata agggagtggg acagaaatca tattttag
//
LOCUS       DALAGF010000088        10542 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.088, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000088 DALAGF010000000
VERSION     DALAGF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10542
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.088"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            46..504
                     /locus_tag="O2692_002103"
                     /pseudo
     CDS             46..504
                     /locus_tag="O2692_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            491..793
                     /locus_tag="O2692_002104"
     CDS             491..793
                     /locus_tag="O2692_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299723.1"
                     /translation="MRTNDHITDLDAIELSSGWNYNSKLETGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINSMSQNEGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            947..1306
                     /locus_tag="O2692_002105"
     CDS             947..1306
                     /locus_tag="O2692_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299724.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALNL
                     LGLATLSLLDTFSCNKK"
     gene            complement(1391..2002)
                     /locus_tag="O2692_002106"
     CDS             complement(1391..2002)
                     /locus_tag="O2692_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCZ4299725.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(2081..2557)
                     /locus_tag="O2692_002107"
     CDS             complement(2081..2557)
                     /locus_tag="O2692_002107"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HCZ4299726.1"
                     /translation="MAVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGQKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(2560..3060)
                     /locus_tag="O2692_002108"
     CDS             complement(2560..3060)
                     /locus_tag="O2692_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4299727.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(3295..4062)
                     /locus_tag="O2692_002109"
     CDS             complement(3295..4062)
                     /locus_tag="O2692_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HCZ4299728.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(4059..4751)
                     /locus_tag="O2692_002110"
     CDS             complement(4059..4751)
                     /locus_tag="O2692_002110"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HCZ4299729.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNRDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(4768..5430)
                     /locus_tag="O2692_002111"
     CDS             complement(4768..5430)
                     /locus_tag="O2692_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HCZ4299730.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
     gene            complement(6278..6838)
                     /gene="icaR"
                     /locus_tag="O2692_002112"
     CDS             complement(6278..6838)
                     /gene="icaR"
                     /locus_tag="O2692_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HCZ4299731.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            7002..8240
                     /gene="pgaC"
                     /locus_tag="O2692_002113"
     CDS             7002..8240
                     /gene="pgaC"
                     /locus_tag="O2692_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCZ4299732.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            8204..8509
                     /gene="icaD"
                     /locus_tag="O2692_002114"
     CDS             8204..8509
                     /gene="icaD"
                     /locus_tag="O2692_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HCZ4299733.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            8506..9378
                     /gene="icaB"
                     /locus_tag="O2692_002115"
     CDS             8506..9378
                     /gene="icaB"
                     /locus_tag="O2692_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HCZ4299734.1"
                     /translation="MKYRKFIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            9365..10417
                     /gene="icaC"
                     /locus_tag="O2692_002116"
     CDS             9365..10417
                     /gene="icaC"
                     /locus_tag="O2692_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HCZ4299735.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDSSFNKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQM
                     ISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIF
                     RFIIGKQPYKLNINAY"
BASE COUNT         3581 a         1735 c         1512 g         3714 t
ORIGIN      
        1 taattaatta tagttacttc aatcatgatt agtttataat aataaagtga aattgaaaaa
       61 gacagctatt atgcgatgag cgaaaaactt caagtaaaac aagaatttaa cggcgttaca
      121 aggcaatact tgaatttcag aacagatagt atcgatgatc tggataagtt caaaaaagaa
      181 ttcaaacctg ttatgcactt aaaaggtgat gcattcaatc aacagttaca atctttgatt
      241 aacaaatatc cacaaataca aaaaaatatg aaatcagagt tcattgctta ttatgataaa
      301 gaaaaaaata gagaaacagt aaaaaactat gcttggaacc ttcaaaaatc tataaatgac
      361 attatgcaat catatcctag cacaaaattt gtacagtttt ataaagatga tgtttccccg
      421 tcaatggtag atggaaatgg ccgtttaaaa tcggatacta atgtaatttc catcgaggga
      481 ggaaagtatg atgagaacaa atgatcatat aaccgattta gatgctattg agttaagtag
      541 cggttggaat tacaattcga aattggagac tggcaaaaat gcactactat cagatagcct
      601 tttaagagat attaaagtat taagttctaa tgctaaaggc gacgctgaca tactagaaac
      661 agaacttcgt gctgtaataa actcaatgtc tcaaaacgaa ggtaaatctg ttgatgcact
      721 taaaaatagt accgcttctc taaaaaacaa tctaaccaaa ttaaaagagc aactcgacaa
      781 aactacacat taatttcata cattttgagt gaacagaatt atatttccat agcaaacatt
      841 cctaaactac acatacgtta cataattgat tcatttttat agaaacgggt aaaaatgata
      901 aagtaatcat ctttcacaaa aaatcaaata ttgggaggaa ttaaatatga ttttgcgtta
      961 cgcaacgaat ttaaaacttg cgaaagagct ataccaagcc agtaaaccta aattgcaaga
     1021 agataagggt atgattgaac tgtttgaaca tacttttggc ttgcaaaaag aacttgtaaa
     1081 atacgtcggc attgcagaag caactactgc tgctttatac tcagctagct ttatcaataa
     1141 aaacatttct cgccttgcat cactatcaac gataggcatt ttaagtgtag ctgcttacaa
     1201 acatttcgaa gctggccaag gtaaaaaagg tgcacaacat gcattaaatt tattaggtct
     1261 tgctacttta agtttactag atacattttc ttgtaataaa aaatagtctt cattttagaa
     1321 gcacatactt tagcacctgt gtacttttga ttcaattcat aaacggtata ccgctatagc
     1381 atgtgtttta ttatttttct ggtctcttta tcgtaaatat tccccctaat ttagcataat
     1441 catttccggc taaacgagaa acagcctgta atgcgtgtgg attgatttta aaatcaccct
     1501 cataaatact gtcatcaata tgataagtta caatttcacc tattattaaa tcagccccat
     1561 ctaatacatc tccaagcaat atcatttgcg atagtttaca ttcgaatctc attttcgcat
     1621 ctttaattcc tggcgtctta atcgttgtag atgttaaaag tgataattct gtacgactca
     1681 actcactgtc accatatgct aacggcgctg cagtctcatt aatatcttga acattatctt
     1741 cgtctgtaat atgcacaaca aagtctccag tccgttctat atttaatgca gtatcttttc
     1801 tcttacctcc tgcacgttga actgcaatag caatcattgg cggatgatta ttaacaatat
     1861 taaaaaagct aaatggtgct gcatttactg atgcatcttg atttaatgtt gtaacaaaag
     1921 ctataggtcg tggaataatt gaaccaatta ataatttata gttttctcta gcagttaatg
     1981 attgtgcatc aaacgtatac ataataccta cctcttttct aagtatatct aggtatttct
     2041 ccgattttgg ttaatttaaa catctattct cctctgaaaa tcacttgtat ttatttagca
     2101 aatcttttga aatatgacac atatgcatat cttctggata tttttctaaa tgttgctgat
     2161 gttcttcagc acttttaatg tagttagaca gcggtaagac ttccactgca atttgatctc
     2221 tgtctttacg tcgttcaatg aactgacgcg cttcaattaa gtggtcatct acacaactat
     2281 ataaacccgt tcgatacttt tgtccaatat catttccttg ttgattcaca ctgtaaggat
     2341 caatgatttc aaataaataa ttcataatgt ctgtaattgt taacatacga tcatcgaaat
     2401 gaagtttgac acattcagca taaccatcat acggaccgtc taatttagag cttcttccat
     2461 ttgctcttcc tgcttctgta tgtataattc caggtattgt tgcaaaaaat gcttcaacac
     2521 cccataaaca tcctcctgct acataaacaa ctgccatatt tacacctcat catccttttt
     2581 tatattttta acaaggttat accatttaat accgccatga catgattctg atacaccttc
     2641 attacgatac ccatattttt cataaaatga aattaatgat tctcgacatg ttaacgttac
     2701 accatgtcga tgatgattct tagcaagagt ttcaaaatag tttagtaagc gacctgcaat
     2761 accctgacct tgataatttg gtgctacaac aagacctaac acactaatat agccaccttc
     2821 actattattt gtggagacat ttttaaataa atcatcgcta atgtaacgct cttttatgac
     2881 tggaccgttg atgtatccaa ttacttcatc attcttttca gctacaataa aagtttcttg
     2941 aatttgttca attctcaatt tcaatgcctc tcgtgttgca gcttcttccg gtgaaaaacc
     3001 aatattttca agtgcaataa tttgatctaa gtcctgcatg attacttttc ttaataccat
     3061 aaaaacactt ccttagccct atatatcaaa agtaatacct tcatctttat attcattatt
     3121 ttcaaactac cttcactata aaaaacgagt ctttttattg aaagccattc gccttaatca
     3181 attagacaag ttgtattata cgaattagta aataatcatg attataattc attttaagca
     3241 tactcaaaaa gactggtaca tgtataccag tctaattcga aaaaatatat ttagttaaaa
     3301 ccatttcaaa aacgacttca cttttcctgg cttagtcggt ctgtctaaat aaacaaaatt
     3361 atcattgatt actgaactgg catttcgaag taatgcttca atatcattcg aatatttctt
     3421 caatttatga ttgtgaaata attcttgcat caaaaatggt ctttggtcac atgaatgtgc
     3481 atctgaagct acaaaatgag ccaaattaca ttctataaat tgtaatgata acttttgaat
     3541 gtttttacca aatccaccaa ctaaagaact cgatgttaat tgactcagtg ccccatttgc
     3601 aaccaattca tataatattt ccggattttt ggcgatactt ctatttcttt caggatgtgc
     3661 aatgattggt atgtaacctc tcgattgtat ttcaaaaaac aattgttttg tataatgtgg
     3721 tacttcgccc gttggaaatt caattaataa atatttcgaa cgattaatac cttgaatact
     3781 accattatct aagcctttca gaatcgaatc tgtaattcta atttcttgcc cgggaaataa
     3841 tttaatatcc aatgcttgaa cttctggatg cgttcttaac tccgccaatt tcacaagcac
     3901 ttgttgaaat gtattatcat atctcggatg caaatgatga ggtgtcgcta caatacttgt
     3961 tacaccttca tccttagctt gctttaatag tgcaatactc ttttcaattg ttttaggacc
     4021 atcatctata tcaactaata tatggttatg aatatcaatc atgattcatc agtcccataa
     4081 tatgcatagt aactagcact tttatcttta ggcattctat ttaagactac acctaataat
     4141 ttagcacctg ttgcttcaat aagttctttt ccttttttaa cttcatctct attattattt
     4201 tccgaattaa ctacgtagac aacattgccg gtaaactttg aaaataattg cgcatctgta
     4261 actgtgttca ctggtggcgt atcgataatt acaaagttat aattcatcaa taatgtgtca
     4321 tacaaatttg caaatgccct tgatgtaatt aactctgacg gattcggtgg gattggccca
     4381 gacgtcaaga cgtctaaatc ttgaatttca gttgagataa tactgtcttg ataagttgac
     4441 caatttagca ataaacttga taggccttca ttgtttggca aattaaaaat ataatgctgc
     4501 gtaggtttac gcatatcccc gtctacgatt agtgttttat aacctgcttg cgcatatgca
     4561 actgctaaat ttgctgcaat tgtagactta cctgcgcctg gtgcctctga agtgattaca
     4621 atgctttgaa ctgcactgtc aggatttgca aacataatat ttgatcttat gcctcgaaat
     4681 ttctcgctaa taggtgactt tggttgttca tggacaatta aacttgatgt acttcttcgt
     4741 gtattcgtca tggtaattcc tcgtaaatta aaatttttgt attgaaccta aaataggtaa
     4801 tcctagttgc gattcaacat cttcttctgt cttaatacgc ttatctaata attcttttaa
     4861 gaaaataatc aatattgcta aaacaatacc aacaataatg ctgataacta agttgacaga
     4921 tactattgga gatactttta cagcattatc atgtgctgag gaaagtatcg taacattatc
     4981 aacactcata attttaggca tgtcatgagc aaaaacttta gatattttat taacaatttt
     5041 gtcagattca gatttattcc cagtggtaac tgatacagta ataatttgag agtttgtttg
     5101 attggttact tttaaaaatg aattcaactc agctgttgaa tactgaccat caaattctct
     5161 agatacttta tctagaattc taggactttt gataatttcc gtatatgtat taacagactg
     5221 caaactactt tgaacatttt ggaaagctaa atcacttgag gactttttca tgttcactaa
     5281 tatttgagta gaagcagtat atttgtcagg cataacaaaa aaggttaatg ccgcacttac
     5341 tacaagacat attgccggta aaataagcaa taatttaata ttcttcttta gaatatttaa
     5401 tagttttact aaatcaaact tttctttcat ggtttcctcc acataatcaa tcattgtatt
     5461 cattatgtat gttttataaa tcggacaatt atatctagtt taacgaccac aaaacataca
     5521 caactacatt ttctctaatt atttatataa atattttatc gtttaaaatt atatcatgat
     5581 tctctaccat tatgtataac ttatttatat ttttgcacaa gatataatat tgtccaactt
     5641 taaatatcca aacctattaa taataaaact agataccatc gtactctatc atggctttct
     5701 tataatcgag tagaagcatc atcattactt gattatttgc tctttacaac accgagcgtg
     5761 cccgtactcg gtaattcaat accttgcgta acccgtcact gtgagttggg ttaatgataa
     5821 taaagcccac accttttaaa aagatgtggg taatttatat aatttttatt tacattttta
     5881 acttataaaa aaagcgccta tgtcatgatt taccatcaca taggcgctta tcaataaatt
     5941 attacttatt actttccatt tcatctaatt tatgcggatt tcctgtaatt agatgacaac
     6001 ttattctttt caggggaaca ttacactttt ataatatgtt caaagacaaa cttaaccatt
     6061 cacaaatata aagaataata ttatcaaatc attgaacaaa tcgtattttg caacaattga
     6121 tatttatatt aatgtattgc atttaattta taaaattcat atacatctta atattctcaa
     6181 tatcgatttg tattgtcaac tttatataga tttaaaaaaa taatctcatg tcttttttta
     6241 caaaagtaag ttaattatta caaactagta acaaaaatta tttcttcaaa aatatattta
     6301 gtagcgaata cacttcatct ttgaattgac ttttactttc ttccactgct ccaaattttt
     6361 gcgaaaagga tgctttcaaa taccaacttt caagaaacag caatattaaa ttctgaaagt
     6421 cttcttttgt catctttatc tttgattcat catagaattt tgctatctct ttacttaatg
     6481 attgatttaa atcttgtatt tgtccgtaaa tatttccaga aaattcctca ggcgtattag
     6541 ataattgaac gtacattcta atataccttt cttcgatgtc gaaaataaac tcaaataaga
     6601 attgatataa agcatcaatt gaatagttcg atttattttg attcatcata ataatattat
     6661 taaggtaatc aaaacaacat ttaacacttt gttcgtaaat actttttttc gagtcaaaat
     6721 ggtaatataa actcgctttc tttatattta cacttttagc tatatcatca agtgttgtac
     6781 cgtcataccc cttctctgaa aataaggtta ttgcgttatc aataatctta tccttcaatt
     6841 tttataaccc cctactgaaa attaatcaca ctatgttaca ggaaaattaa gttgcaatta
     6901 caaatatttc cgtttaatta taacaacaat ctattgcaaa ttaaaatact atcaattacc
     6961 atatggctta caacctaact aacgaaaggt aggtaaagaa attgcaattt tttaactttt
     7021 tgctttttta tcctgtattt atgtctattt actggattgt cggttcaatt tatttctatt
     7081 ttaccagaga aattagatat tcattgaaca agaagcctga cataaatgtg gatgaattag
     7141 aaggcattac atttttactt gcctgttata acgaaagtga aacgattgaa gatacgttgt
     7201 ctaatgttct tgcactcaaa tacgagaaga aagaaattat tatcattaat gatggaagtt
     7261 cagataatac agcagaactc atctataaaa tcaaagaaaa taatgacttt attttcgtcg
     7321 atttacaaga aaacagaggt aaagccaacg cactcaatca aggcattaaa caggcttcat
     7381 atgattatgt aatgtgcttg gatgcagata ctatcgttga tcaagatgca ccatattata
     7441 tgattgagaa tttcaaacat gatccaaaac ttggtgcagt tacaggtaat cctagaattc
     7501 gaaataagag ttctatttta ggtaaaattc aaacgataga atatgcaagt ttaattggct
     7561 gtattaagcg aagtcagaca cttgctggcg cagtcaatac tatttcgggt gtcttcactc
     7621 tatttaaaaa aagtgcagtt gtcgacgttg gctactggga tactgatatg attaccgaag
     7681 atattgcagt ttcttggaaa ttgcatttac gtggatatcg tattaagtat gaaccgcttg
     7741 ccatgtgttg gatgttggtt ccagaaacat tgggaggtct ttggaagcaa cgcgtgagat
     7801 gggctcaagg gggacacgaa gtattactac gagacttttt tagcacaatg aaaacgaaaa
     7861 ggtttccttt atatattttg atgtttgagc aaatcatctc gattttatgg gtatatatag
     7921 tgcttctata tttaggctat ttgttcataa cagcaaactt cttagactat acatttatga
     7981 catatagttt ttcaatattt ctactatcat catttactat gacttttata aacgttattc
     8041 aatttacagt cgcactcttt attgatagtc gctacgagaa aaagaatatg gctggactca
     8101 tatttgtaag ttggtatccg acagtatact ggattattaa cgcagcagta gttcttgtcg
     8161 catttccaaa agcattaaaa cgtaagaaag gtggttacgc aacatggtca agcccagaca
     8221 gagggaatac ccaacgctaa aatcatcgct aaatattgta agagaaacag cacttatcgc
     8281 tatatcttgt gtcttttgga tatattgttt agttgttcta ctcgtttata ttggtactat
     8341 atttgaaatt catgacgaaa gtatcaatac aatacgtgtt gctttaaaca ttgaaaatac
     8401 tgaaatttta gatatatttg aaactatggg cattttcgcg attatcattt ttgtattttt
     8461 tacaattagc atattgattc aaaaatggca gagaggaaga gaatcgtgaa gtatagaaaa
     8521 tttataattt tagtgttgag tatcttgatc atattgcctg taagcacact ggatggtcat
     8581 catattgcaa atgcagatga cgattcacct aaaaaactga aatataaaga aaatagtgct
     8641 ctggcattaa attatcaccg tgtaagaaaa gcgaattttc tgaataattt tatttacttc
     8701 ttttctagta gtaaagaaat taaaaattat agtgttagtc aatcacaatt tgaatctcaa
     8761 ataaaatggc taaaatcaca tgatgctaaa tttttaacct tgaaagaatt tttatattac
     8821 aagaaaaaag gtaagtttcc aaaacgaagt gtatggatta actttgatga tatggatgaa
     8881 actatttatg aaaatgctta tccaatctta aaaaaatata aaataccggc aactgggttt
     8941 attatcacag gtcatgttgg ggaagaaaac tttcacaacc tcgatatgat tagtaaaaaa
     9001 gaactaaaag aaatgtataa aactgggtta tgggaatttg aaacacatac ccacgatttg
     9061 cataacttat ctaaaaataa taagtcaaaa ttaatgaaag cttctgaagc tacaatcata
     9121 aaagatttaa acaaaagtga aaaatatcta actaaaaact ttaaaaagtc gcagaaaact
     9181 atagcctatc cttatggctt gatgaatgac gataaattac cggtaatcaa aaaagctggg
     9241 ttaaaatacg gtttttcatt agaggaaaaa gcagtcactc cgaactccaa tgattattac
     9301 atccctagaa tattaattag tgatgatgct tttgagcatt taattaagag atgggacgga
     9361 ttccatgaaa aagattagac ttgaactcgt atatttacgt gctattatat gtgcaattat
     9421 tattatcaca catttactta cacaaattac tttaaaacat gaaaatatgg agggtggatc
     9481 cttagtgtta caattttaca ttcgtaatat tgtgattttt ggtacacctt gctttattat
     9541 cttgtcacag ttactgacaa ccttgaatta ccaaaaagtc acctatagat acttaactac
     9601 acgcgtaaaa tatatactta ttccttacat attaatggga ttgttttaca gttatagtga
     9661 atcattatta acagattcaa gtttcaataa acaattcatt gaaaatgtcc tattaggtca
     9721 atggtatggc tattttatcg ttgttatcat gcaattcttt attttgagtt atatcatttt
     9781 taaaattaac tataacctat tcaacagtaa aatattatta ttgttatctt ttattttaca
     9841 gcaatcattt ttatattact ttacgaacaa cacagcgttt cacgataccg tgctacacta
     9901 ttatccatta agtgaaaata ctataatatt cggatggatt ttttatttct tcttaggtgc
     9961 atatatgggt tataactacg aacgtgtatt aaatttctta gaacgttatt tagttattat
    10021 gattgtatta gctgtagcta cttattttgt gtttattgcg ttagcaaatg gagactattg
    10081 gaacgttacc agcttttcat attcattaac accatataat agtattatgt ttattgttat
    10141 cttgggtatt tgcacgcatt ttaaaacaat gttatttaat acgattcaaa tgattagtgc
    10201 tttctcattc tttatttatt tattacatcc aatcattcta gactcattgt ttgcatatac
    10261 aaatatattt gaggataata caatggtctt tctagcgata tcactactat tcattttagg
    10321 attatgtata ggtgtcggca tgatattgcg tgaattctat atctttaggt ttattattgg
    10381 aaaacaacca tataaattga acattaatgc ttattaatta ttaagctatg ttaaaaacac
    10441 gcggtgggcg aaatcagttt gaattgactg acttcgtttt accgcgtgtt taatattgtt
    10501 atacatatat tctaattgca catttaaact tcgtaaatgc ca
//
LOCUS       DALAGF010000089        10437 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.089, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000089 DALAGF010000000
VERSION     DALAGF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.089"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            29..1510
                     /locus_tag="O2692_002117"
     CDS             29..1510
                     /locus_tag="O2692_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCZ4299736.1"
                     /translation="MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYK
                     GNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEI
                     EESKGISRYFDITTGTYIAYIRKSKSEKVIDFFKDNKRIERFSFIDNKVHMKETFNVD
                     NKVCYQVFYDEKGYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDS
                     KDSIMICDGPGSFPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKI
                     NGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRID
                     LLIEVAELVVKKDNAVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLED
                     FKLVVSTSQYEGQGLSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNINDMA
                     DKILQLVNNDVLAAEFGSKARENIIEKYSTESILEKWLNLFNS"
     gene            complement(1599..1955)
                     /locus_tag="O2692_002118"
     CDS             complement(1599..1955)
                     /locus_tag="O2692_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCZ4299737.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
     gene            2432..3391
                     /locus_tag="O2692_002119"
     CDS             2432..3391
                     /locus_tag="O2692_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ4299738.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(3437..3568)
                     /locus_tag="O2692_002120"
     CDS             complement(3437..3568)
                     /locus_tag="O2692_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299739.1"
                     /translation="MRYVTMRQFIKRTVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(3632..3847)
                     /locus_tag="O2692_002121"
     CDS             complement(3632..3847)
                     /locus_tag="O2692_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCZ4299740.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            4012..4563
                     /locus_tag="O2692_002122"
     CDS             4012..4563
                     /locus_tag="O2692_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4299741.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWELLDLPEKIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(4615..5553)
                     /locus_tag="O2692_002123"
     CDS             complement(4615..5553)
                     /locus_tag="O2692_002123"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCZ4299742.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            5735..7096
                     /locus_tag="O2692_002124"
     CDS             5735..7096
                     /locus_tag="O2692_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCZ4299743.1"
                     /translation="MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGGITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            7083..8756
                     /gene="menD"
                     /locus_tag="O2692_002125"
     CDS             7083..8756
                     /gene="menD"
                     /locus_tag="O2692_002125"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCZ4299744.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTVNRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            8743..9546
                     /gene="menH"
                     /locus_tag="O2692_002126"
     CDS             8743..9546
                     /gene="menH"
                     /locus_tag="O2692_002126"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCZ4299745.1"
                     /translation="MTHYKFYEANVETNQVLVFLHGFLSDSRTYHNHIEKFTDNYHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            9539..10360
                     /gene="menB"
                     /locus_tag="O2692_002127"
     CDS             9539..10360
                     /gene="menB"
                     /locus_tag="O2692_002127"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCZ4299746.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
BASE COUNT         3910 a         1414 c         1913 g         3200 t
ORIGIN      
        1 aatatactga ggtaaaggaa taattataat gaaaaaaata tttatgatgg tacatgagtt
       61 agatgtgaat aaaggtggta tgacctcttc gatgttcaat agaagtaaag agttttatga
      121 tgcggacata cctgctgata ttgttacttt cgattacaaa ggaaactatg atgaaattat
      181 taaagctttg aaaaaacaag gtaaaatgga tcgaagaacg aaaatgtata atgtatttga
      241 gtattttaaa caaatttcaa ataataaaca ttttaagtct aataaattgt tatataaaca
      301 tatttcagaa agactaaaaa atacgattga aattgaagag agtaaaggta tttcaagata
      361 ttttgatata acgactggta catatattgc ctacattaga aaaagtaaat ctgaaaaagt
      421 gattgatttc tttaaagata ataaacgaat tgaacggttt agttttatag ataataaagt
      481 gcatatgaag gaaacattta atgtagataa taaagtttgt tatcaagtat tttatgatga
      541 aaagggatac ccatatattt caaggaatat taatgctaat aatggtgctg taggtaaaac
      601 ttatgtgtta gttaataaaa aagaatttaa aaacaattta gcactgtgtg tttactattt
      661 agaaaaacta ataaaagatt ctaaagatag tattatgatt tgtgatggac cagggagttt
      721 tccaaaaatg tttaatacaa atcataaaaa tgctcagaaa tatggcgtta ttcatgttaa
      781 tcatcatgaa aatttcgatg atacgggtgc atttaaaaaa agtgagaaat atattattga
      841 gaatgcgaat aaaattaacg gtgtaattgt attaacagag gcacaaagat tagatattct
      901 taatcaattt gatgtagaaa atattttcac tattagcaat tttgttaaga tacataatgc
      961 tccaaaacat tttcaaactg aaaaaatcgt aggtcatatt tctagaatgg taccaacgaa
     1021 gcgaattgat ttgcttattg aagtggctga gttagtcgta aaaaaagata atgctgttaa
     1081 atttcatata tatggagaag gatctgtcaa agataaaata gctaaaatga ttgaagataa
     1141 aaatttagaa agaaatgttt ttcttaaagg atatacaaca actccacaaa aatgcttgga
     1201 agattttaaa ttagtcgttt ctacatctca atatgaaggt caagggttaa gtatgataga
     1261 agcaatgatt tctaaaaggc ctgttgttgc ctttgacatc aaatacggac caagtgattt
     1321 tatagaagat aataaaaatg gttatttaat agaaaaccat aatattaacg acatggctga
     1381 taaaatactt cagcttgtta ataatgatgt attagcagcg gagtttggtt cgaaagcgag
     1441 agaaaacatt atagaaaaat attcaacgga atcaatatta gaaaaatggt taaatctttt
     1501 caatagctaa atgaaaaaat cctaggtatt catgagtacc taggattttt acgcatgata
     1561 aatacaaaaa aagagaggat agtcctctca aacaatagtt aataaaattg tagccatttc
     1621 ttccagctgt aactactaat acaagctaag ttcattacga tatgtatgat ttcaaattca
     1681 taaccactta catatcctct tgtaagttta agtgtgaaga ttgagcctaa cataacacaa
     1741 atataaaaaa cattaacata aggcgttaaa aagccgataa taagtgctaa acctccaaca
     1801 acttcaccaa ttgacatcaa tactgcgatg gtaggtggta aacctaaaga gcctaagaag
     1861 tgaattgttc catctaaatc aacaaatttc aaagtaccat gcactaagaa tgatgttgct
     1921 aacatccatc taattaaaag catacctaat ttcatcatgt cacctccaac attcatattt
     1981 ttaaaacata gtccaaatta tatttgtaaa catgtggaaa gtaaagattt tttataagat
     2041 taattattaa atattctatt gtattgaaca attgttgtag aaagatgttt aagtcatcag
     2101 ttactttttg tatttataaa taaagattag tacagcatag tgcaaagatt atagggaaat
     2161 gaattaacta ttgcatcgct atttatgaat cgtaaacact attatgaaat tgcgagagta
     2221 gtatggacaa ttagaatgat ggtttgagat aaatgagtgt tattaaccag tgcgtcattt
     2281 tcaaaattag atgccatttg ctgtttataa taagaagatt tgataattaa ctatgataag
     2341 aaaaaataat aatcccctaa atacattata aacaaattaa atgataaaat aataatatat
     2401 atttctacta aagttgtttg gaggtaaatg tgtgaatagg aatatcgtta aactagttgt
     2461 gttcatgcta attttagttg tagcagtagc gggttgtggt caaaaagata ctgaagagaa
     2521 aactgaaatg acgacaataa aagatgaatt aggaactgaa aaaattaaga aaaatcctaa
     2581 acgtgttgtt gtattagaat atagttttgc tgattattta gcagcattag atatgaaacc
     2641 tgttggtatt gcagatgatg gcagcactaa aaatataaca aagtcagtaa gagataagat
     2701 tggggcatat gaatcggttg gatctagacc gcaaccgaat atggaagtga taagtaaatt
     2761 aaaaccggat ttgatcattg cagatgttag cagacataag aaaatcaaat cagaattgag
     2821 caaaattgct ccgacaatca tgttagttag cggtacggga gattataatg caaatattga
     2881 agcatttaaa acagtcgcta aagcagtagg caaagagaaa gaaggcgaga agcgtctgga
     2941 aaagcatgat aaaatattag cggagattag aaagaaaatt gaacagagta cgttaaaatc
     3001 tgcatttgca ttcggtatct caagagcagg tatgtttatt aataatgaag atacatttat
     3061 gggacaattc ttaattaaaa tgggtattca acctgaagtc acaaaagaca aaactacgca
     3121 tgttggtgaa cgcaagggtg gtccttatat atatttaaat aatgaagaac ttgccaatat
     3181 caatccaaaa gttatgattt tagccactga cggaaaaacg gacaaaaata gaacgaaatt
     3241 cattgatcct gcagtttgga aatcattaaa agctgtgaaa gataacaaag tttatgacgt
     3301 tgaccgaaat aagtggttga aatcaagggg gattatcgca agtgaaagta tggcagaaga
     3361 tttagaaaaa attgcagaaa aagcaaaata aaaatacagc gctactcgta aatcatataa
     3421 gagtggcgct gtatttttaa ttatgtttat tatctgtcgg atgtgatgat ttacctgaaa
     3481 gtttatttcg aataaattta attacataac cgacaaggat tgttttaaca gttcttttaa
     3541 tgaattggcg catcgttaca tacctcattt ctctatatct tacgaactat atacccattc
     3601 atatatgctt tttaaacgtc attgtcacaa tttaattttt agggaatata atataaccat
     3661 ctttatctgc ttttttagta aaaatgacaa aaattgcatg tattattgag atgatggtag
     3721 ggatacctgt ccagaaaaat aataagtgaa aaagaccttg tccaaattta tcagcataaa
     3781 atttatgaat acctaaacct ccaagaaata atgcaacaat aacataaatg gctttattga
     3841 ctttcatttg taaatcctcc ttaactataa ttctacttaa attcgttgtg aaaaccaata
     3901 tttctaactt tagaattttc aaactttcta aaattataag tatatctttt taaaataagc
     3961 tagaatttct atataataaa tgttaataac gtaaaaggga atgatgacat agtgatacgt
     4021 caagcacgtc cagaggaccg atttgatatt gcgaagttag tttatatggt ttgggatgat
     4081 atggaattag aattggtaaa gcatctacct aaagacatgg tattagatgc aattgaaaaa
     4141 agctgtgttg atgcaacata tcgaactttt tatcagcata ttttagttta tgaagtagaa
     4201 aataaagtag caggttgtat tattagctat agtggtgaaa atgaattgaa atacgaaaaa
     4261 gcatgggaac tacttgactt gccagaaaaa ataaaacaat atggcacgcc attacctgta
     4321 aaagaagcta aagacgatga gtattatata gaaacaattg cgacatttgc agcatataga
     4381 ggtagaggca tcgcgacaaa gttattaacg tcattacttg aatcaaatac acatgttaaa
     4441 tggagtttga attgcgatat taataatgaa gcagcattaa agttatataa aaaagtaggc
     4501 tttatatctg atggacagat tgaattatac aagcacatgt atcatcattt aattgttaaa
     4561 taaaaaaact cgacagttcg atgtaagtcg attgccgagt agtatcattt tctattaaat
     4621 gcctgcaaat aatgcactaa tataaatacc taatgcatat aataaaccga aaaatgtatt
     4681 tgttttacca gcagcagcca ttgctggcat cattgtaggc ggtgtatcat tcttcttgaa
     4741 acgtctgata actttaacag gcattgggaa tgataacaac gcaagtaagt aaaataatga
     4801 gccaccaggt ttaataatga tcgtaagtac aataaaggca taagcgataa agtacatgat
     4861 tgccataaat gttaaagaag catttttacc taatagaatg ggtaaagttt tgcgaccact
     4921 tgctttatct ttgacacggt cgcgaatatt gttagccata ttaattaaac cgatagtgat
     4981 tactataggt acacttaacc aaattacata actttgaata ttgccagttt gaataaagaa
     5041 tgcaataacg ataataaaca tacccataaa tacgcctgag aataattcac cgaaaggcgt
     5101 ccatgaaata gggaaagggc cacctgtata taggtaacca acagccatac atactaatcc
     5161 aactggtaat aaccaaaatg aagagttagc agctaaaaac aaacctaata ttgctgctaa
     5221 gatgtaaaat gcaatggcta atcgtagcac aagctctggg ctcataccgt tgcgaacaat
     5281 ggcaccacca atgcctacag attcatgatc atcaaggcct tttttataat catagtattc
     5341 attaaacata ttagttgctg cttgaataag taagcatgct agtaacatgg caatgaatag
     5401 gctgatttta atatgatctt cgctaccaag aaaatatatt ttagatgctg ctgtaccaac
     5461 taaaacgggt actacggaag cagttaatgt atgaggacgc attaaatgcc aatatttctt
     5521 aactgtagaa tattgctgat attgattact cattgaatac aaaacctctt taaatcatat
     5581 tttttataac aaagatattt taatagaaag tagcataaag gtcaattgac gtgaaaaatt
     5641 gcaagataag tttaagcaat tcttgtaaca caattatcca aaaatgatac aatataataa
     5701 tgagtgaaaa aagacgaaga aagaagtgaa atggatggct acgggcgtat tagaggacga
     5761 tattgtcaaa gagatatatg gcagctcaaa ggaatgggtt tcagttgaag tgaaattatc
     5821 acagtcacta gacccgagca cattatttca tctcactgac aatgaggcag gagatcgctt
     5881 ttatatgcgt ttgaatgata atcgaacgtc atattttggc tacaaagcaa ttcaattatt
     5941 caaaaataat tctaaaaata aacaatctat ttttaaagac tgggaaaaat taaaacataa
     6001 catcacattt atacatccgc aatctgagaa acatcatctt cgagttgttg gagggttcca
     6061 attttcaagt cataaatcag atgatgaatg gcgagagttt ggactaaatc attttgtatt
     6121 acctgaagtt ttaatttcaa ctgataataa tgggacattt ttaacttata cagttaaaag
     6181 ggaaagtttt actgttgagg cattgaacga tttaatggat ttgttcaaca atatatcgga
     6241 catagatgtg gacgagcaaa ttgggggaat tactagaaat gaagatattt ataaagatga
     6301 ctggcgtcaa cttgtagtag aagctataga atctattaat aatgaagaaa aaattgtact
     6361 agcacgtaga cggttaataa agttcgataa agatatcagt attccatata ttctaaagca
     6421 agcatattct aaagaaaaaa acagttatat attcttgtta gaatcacaag attctatatt
     6481 cttttcacaa acacctgaac aattaataaa ggtcaataat aaaatactat cgactaaagc
     6541 tgtagcaggt acaattaaac gttcacaaga tgaggacgaa gatacaaaaa atgttgaagc
     6601 atttttaaaa gataataaaa acttaatcga acatcgattt gttgttgaca gtattttaca
     6661 tgatattaaa ccttatatca ctgaattaca ttatgataag acgcctaaaa ttctaaaaaa
     6721 tgatcattta tatcacttgt acactgaaat aaaggcgcca ctgaaggatg attcgtatat
     6781 tagtttaatt gataatttac atccaacacc tgctttaggt ggctatccaa aagaatttgc
     6841 aatggatttt attgagcaga aagaatttgg tacgcgagga ttatatggtg caccggttgg
     6901 ctatatagat atatacgatg attgtgaatt tattgttgca attcgttcga tgcttattaa
     6961 gaaagcacaa gcaactttat ttgctgggtg tggcattgtt aaagattctg atccagatag
     7021 tgaattggca gaaacgaacc ttaagttcac acctatgatg aatgcattag gagtcgatat
     7081 gaatgggaaa tcataaagca gctttaacga agcaagtttt tacatttgca tctgagttat
     7141 atgcgtacgg cgtaagggaa gtagttatca gtccgggatc acgctcaacg ccacttgcac
     7201 ttgcatttga agcacatcca aatattaaaa catggataca ccccgatgag cgaagtgcag
     7261 cgttttttgc agttgggtta attaaaggca gtgaaagacc tgtcgctata ttatgtacgt
     7321 caggtacagc agcagcgaat tatacgcctg caattgctga aagccaaatt agtagaattc
     7381 ctttaatcgt tttaacaagt gaccgtccgc atgaattaag aagtgtaggc gcaccacaag
     7441 cgattaatca agtaaatatg tttaataatt atgtaagtta tgagttcgat atgcctattg
     7501 cggatgatag taaagagacc attgatgcaa tttactatca aatgcaaatt gctagtcaat
     7561 atttatatgg accacataaa gggccaattc attttaactt gccatttaga gatccgttaa
     7621 cacctgattt gaatgcaaca gaattgttaa cttctgagat gaagatttta ccgcactatc
     7681 aaaaaagtat agatgcatcg gcattaagac acattttaaa taagaaaaaa ggtttaatta
     7741 ttgtagggga tatgcagcac caagaagttg atcaaatact aacgtattca acgatatatg
     7801 atttgcctat tttagctgat cctttaagtc atttaagaaa atttgatcat ccgaatgtta
     7861 tctgtacata tgatttgctg tttagaagcg gcttagactt aaatgtggat ttcgtaattc
     7921 gtgttgggaa accagtgatt tctaaaaagt taaatcaatg gttaaagaaa actgatgcat
     7981 ttcaaatatt agtgcaaaac aatgataaga ttgatgtctt tccgatagcg ccagatattt
     8041 catatgagat ttctgcgaat gatttcttta ggtcattaat ggaagacacg acagttaatc
     8101 gcgtaagttg gttagaaaaa tggcaatgct tagagaaaaa agggcgtaaa gaaattaaat
     8161 gttatttgga acaagctaca gatgagagtg cattcgttgg tgaattgatt aagaaaacat
     8221 ctgaaaaaga tgcattattt attagtaata gtatgcctat cagagatgta gataacttgt
     8281 tattgaataa aaatatagat gtctatgcga atcgtggtgc gaatggtatt gatggtatcg
     8341 tttcaactgc actgggtatg gctgtgcata aacgaataac attattgata ggtgatttat
     8401 cattttatca tgatatgaat ggactattaa tgtcaaaatt aaataatatt cagatgaata
     8461 ttgtattatt gaacaacgat ggtggcggta ttttttcata tttaccacaa aaagaaagtg
     8521 caactgacta ttttgaacgg ttgtttggca caccgacggg attggatttc gagtatacag
     8581 ctaagttata tcaattcgat tttaaacgtt ttaacagtgt ttcagaattt aaaaatgcca
     8641 cattgttatc tgaaacttcg acgatttatg aattgataac gaatcgcgaa gataacttta
     8701 aacagcatca aattttatac cagaaattga gtgaaatgat tcatgacaca ttataaattt
     8761 tatgaagcaa acgttgagac caatcaagtt ttagtatttc tgcatggatt tcttagcgac
     8821 agccgtactt atcataatca catcgaaaaa tttactgata actatcatgt catcactata
     8881 gacttaccag gccatggcga agatcagtct tcaatggatg aaacgtggaa ttttgattat
     8941 attacgacgt tgttagaccg aattttagat aaatataaag ataaatcaat aacattgttt
     9001 ggatattcaa tgggtgggcg tgttgcatta tattatgcaa ttaatggtca catccctata
     9061 tctaatttga tattagaaag tacgtcacca ggtattaaag aagaagcaaa tcaattggaa
     9121 cgccgtcttg ttgatgatgc acgtgctaaa gtattagaca tagcaggtat tgaattattt
     9181 gttaatgatt gggaaaagtt gccattattt caatcgcaac tagaattacc agttgaaata
     9241 caacatcaaa taagacaaca acgattgtct caatcgccac ataaaatggc caaagcatta
     9301 agagattatg gtacaggtca aatgccaaac ttatggccgc gcctgaaaga aattaaagta
     9361 ccaacattaa tattagctgg agaatatgat gaaaaatttg tacagattgc gaaaaaaatg
     9421 gcaaatttaa ttcctaatag taaatgtaaa ttaatttctg ctacaggtca tacaattcat
     9481 gtggaagata gtgatgaatt tgatacaatg atattaggat ttttaaagga ggagcaaaat
     9541 gactaacaga caatgggaaa cacttagaga atatgatgaa atcaaatatg aattttacga
     9601 agggattgct aaggtaacaa taaatcgccc tgaagtacgc aatgcgttta cacctaaaac
     9661 agttgctgaa atgattgacg cattttcacg tgcacgtgat gatcaaaacg tttcagttat
     9721 cgtattaact ggtgaaggtg atttagcatt ctgttctggt ggtgaccaga agaaacgtgg
     9781 acatggtggt tatgtaggtg aagaccaaat ccctcgctta aatgtattag atttacagcg
     9841 tttaattcgt attattccaa aaccggttat cgcgatggta aaaggttatg ctgtaggtgg
     9901 cggtaatgta ctaaatgttg tttgtgactt aacgattgct gctgataatg ctatttttgg
     9961 acaaactggt cctaaagtag gttcatttga tgcgggttat ggttcaggat atttagcacg
    10021 tatcgttgga cataagaaag cacgtgaaat ttggtactta tgtcgtcaat acaatgcaca
    10081 agaagcttta gatatgggtc tagtaaatac agtggtacct ttagagaaag ttgaagatga
    10141 aactgtgcaa tggtgtaaag agattatgaa acactcacca acagcgttac gattccttaa
    10201 agcagctatg aatgctgaca cagatggttt agctggttta caacaaatgg ctggggatgc
    10261 aacattgctt tattacacaa ctgatgaagc gaaagaaggc cgtgatgcgt ttaaagaaaa
    10321 acgtgatcct gacttcgatc aattccctaa attcccataa gttattttga atatgatatt
    10381 aagtcacttg cgtcgtttat tagcgacgat aggtggcttt tttattttat aagaatt
//
LOCUS       DALAGF010000090        10389 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.090, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000090 DALAGF010000000
VERSION     DALAGF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10389
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.090"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..576
                     /gene="capA"
                     /locus_tag="O2692_002128"
     CDS             <1..576
                     /gene="capA"
                     /locus_tag="O2692_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HCZ4299747.1"
                     /translation="SAIVTFFVLSPKYQANTQILVNQTKGDNPQFMAQEVQSNIQLVN
                     TYKEIVKSPRILDEVSKDLNDKYSPSKLSSMLTITNQENTQLINIQVKSGHKQDSEKI
                     ANSFAKVTSKQIPKIMSVDNVSILSKADGTAVKVAPKTVVNLIGAFFLGLVVALIYIF
                     FKVIFDKRIKDEEDVEKELGLPVLGSIQKFN"
     gene            592..1278
                     /gene="cap8B"
                     /locus_tag="O2692_002129"
     CDS             592..1278
                     /gene="cap8B"
                     /locus_tag="O2692_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HCZ4299748.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            1281..2045
                     /gene="cap8C"
                     /locus_tag="O2692_002130"
     CDS             1281..2045
                     /gene="cap8C"
                     /locus_tag="O2692_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HCZ4299749.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMMDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVINGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            2065..3889
                     /gene="cap8D"
                     /locus_tag="O2692_002131"
                     /pseudo
     CDS             2065..3889
                     /gene="cap8D"
                     /locus_tag="O2692_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
     gene            3879..4907
                     /gene="cap8E"
                     /locus_tag="O2692_002132"
     CDS             3879..4907
                     /gene="cap8E"
                     /locus_tag="O2692_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HCZ4299750.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKQFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVYYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            4920..6029
                     /gene="cap8F"
                     /locus_tag="O2692_002133"
     CDS             4920..6029
                     /gene="cap8F"
                     /locus_tag="O2692_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HCZ4299751.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            6033..7157
                     /gene="cap8G"
                     /locus_tag="O2692_002134"
     CDS             6033..7157
                     /gene="cap8G"
                     /locus_tag="O2692_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HCZ4299752.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGL
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            7160..7786
                     /locus_tag="O2692_002135"
     CDS             7160..7786
                     /locus_tag="O2692_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCZ4299753.1"
                     /translation="MRIAIEKIIGLLKNQSSKESNVKIHRLAYITNSKFDGNNYIDRW
                     CKIRNSHIGEYSYIGFGSDFNNVEVGRYCSISSDVKIGLGKHPTHFFSSSPIFYSNNN
                     PFNIKQKFIDFNDQPSRTTIKNDVWIGANVIIMDGLTINTGAVIAAGSVVTKNVGAYE
                     VVGGVPAKVIKKRFDNKTIEKLLESKWWEKTPDKLKGFSVEYLNKKDT"
     gene            7791..8900
                     /locus_tag="O2692_002136"
     CDS             7791..8900
                     /locus_tag="O2692_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCZ4299754.1"
                     /translation="MRILNIVSSNIVQDPRVLKQIETIKGVTDDYKIVGMNNSQATNK
                     RLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAYKPDVIHANDFDVLLMV
                     YLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKE
                     YYQSKGYKKEANVITNAPILNDSREFKEIENFKEIVYQGQIVMDRGYEEFIIASSAFK
                     QNAPSFIIRGFGPHEEVIKELISYNPENIRLDKPVEVKELVDKLAESNVGVVLTKPVS
                     INFEYTVSNKIFECIHAGLPVILSPVKEHIYLNEKYKFGIVLKEVTPLEIEKAVRKLR
                     DNHDLFNHLRQNAIKASKILNWQIESERLVELYKF"
     gene            8914..10080
                     /locus_tag="O2692_002137"
     CDS             8914..10080
                     /locus_tag="O2692_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCZ4299755.1"
                     /translation="MKFFVLCAIISMNIFIVISTFTKEVLGFPIEPVYYSTMVGIALI
                     TTVFAIYKIIVTQEIPRGLILLIAICLLYLAFYYFSPDKEEKLAKNNILFFLTWAVPA
                     AISGIYIKYINKATVERFFKLVFFIFSVSFIFVILIPKLTGEIPSYINFGLMNYQNAS
                     YLSAFTAGLGIYFIMKGSVKHKWIYVLFTIIDIPIVFIPGGRGGAILLILYGLFAFIL
                     ITFKRGIPIAVKSIMYIFALSISSVLIYFLFTKGSNTRTFSYLQGGTLNLEGTSGRGP
                     IYEKGIYFIQQSSLLGYGPFNYYKLIGNIPHNIIIELILSFGLLGFFIIMICILLLVY
                     KMIRNYDPNTIDLLVMFIAIYPITLLMFSSNYLVVSEFWFVLFYFITKGRRHHG"
     gene            10073..>10389
                     /locus_tag="O2692_002138"
     CDS             10073..>10389
                     /locus_tag="O2692_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCZ4299756.1"
                     /translation="MAKKVFIMDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSI
                     LTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFASILLISILIESIALIIVFL
                     YFF"
BASE COUNT         3756 a         1419 c         1977 g         3237 t
ORIGIN      
        1 agcgctattg ttacattttt cgtcttatca cctaaatatc aagctaatac tcaaatttta
       61 gtgaatcaaa ctaagggtga caatcctcag tttatggcgc aagaggttca aagtaatatt
      121 caacttgtaa atacgtataa agaaattgtt aaaagtccta gaattttaga tgaggtgtca
      181 aaggacttaa atgataagta ttcaccatct aaattgtcga gtatgttgac aattacaaac
      241 caagaaaata cgcaacttat caacatccaa gttaaaagtg gtcataaaca agattcggaa
      301 aaaattgcga atagcttcgc taaagttaca agtaaacaaa ttccgaagat tatgagtgtg
      361 gataacgtat caattttatc taaagcagac ggtacagcag ttaaagtcgc accaaaaact
      421 gtagtgaatc taatcggtgc attcttttta ggattagttg tcgcgcttat atatatcttc
      481 ttcaaagtaa ttttcgataa gcgaattaaa gatgaagaag atgtagagaa agaattagga
      541 ttgcctgtat tgggttcaat tcaaaaattt aattaaggat ggttgctact tatgtcaaaa
      601 aaggaaaata cgacaacaac actatttgta tatgaaaaac caaaatcaac aattagtgaa
      661 aagtttcgag gtatacgttc aaacatcatg ttttcaaaag caaatggtga agtaaagcgc
      721 ttattggtta cttctgaaaa gcctggtgca ggtaaaagta cagttgtatc gaatgtagcg
      781 attacttatg cacaagcagg ctataagaca ttagttattg atggcgatat gcgtaagcca
      841 acacaaaact atatttttaa tgagcaaaat aataatggac tatcaagctt aatcattggt
      901 cgaacgacta tgtcagaagc aattacgtcg acagaaattg aaaatttaga tttgctaaca
      961 gctggccctg tacctccaaa tccatctgag ttaattgggt ctgaaaggtt caaagaatta
     1021 gttgatctgt ttaataaacg ttacgacatt attattgtcg atacaccgcc agttaatact
     1081 gtgactgatg cacaactata tgcgcgtgct attaaagata gtctgttagt aattgatagt
     1141 gaaaaaaatg ataaaaatga agttaaaaaa gcaaaagcac ttatggaaaa agcaggcagt
     1201 aacattctag gtgtcatttt gaacaagaca aaggtcgata aatcttctag ttattatcac
     1261 tattatggag atgaataagt atgattgata ttcataacca tatattgcct aatatcgatg
     1321 acggtccgac aaatgaaaca gagatgatgg atcttttaaa acaagcgaca acacaaggtg
     1381 ttacagaaat cattgtaaca tcacatcact tacatcctcg atataccaca cctatagaaa
     1441 aagtgaaatc atgtttaaac catattgaaa gcttagagga agtacaagca ctaaatctaa
     1501 agttttatta tggtcaggaa ataagaatta ccgatcaaat ccttaatgat attgatcgaa
     1561 aagttattaa cggtattaat gattcacgct atttactaat agaatttcca tcaaatgaag
     1621 ttccacacta tactgatcaa ttatttttcg aattacagag taaaggcttt gtaccgatta
     1681 ttgcacatcc agagcggaat aaagcaataa gtcaaaacct tgacatacta tacgatttaa
     1741 ttaacaaagg tgctttaagt caagtgacaa cggcgtcatt agcgggtatt tccggtaaaa
     1801 aaattagaaa attagcaatt caaatgattg aaaacaatct gacacatttc atcggttcag
     1861 atgcgcataa cacagaaatc agaccgttct taatgaaaga cttatttaat gataagaaat
     1921 tacgtgatta ttatgaagat atgaacggat ttattagtaa tgcgaagtta gttgttgatg
     1981 ataaaaaaat tcctaaacga atgccacaac aagattataa acagaaaaga tggtttgggt
     2041 tataaacagc aaatgagggg ttttatggca catttatctg tgaaattgcg gcttttaata
     2101 ctagcattaa tcgattcact gatagtgaca ttttcagtat tcgtaagtta ttacatttta
     2161 gaaccgtatt tcaaaacata ttctgtcaaa ttattaatat tggcagctat atcactattc
     2221 atatcgcatc atatttcagc atttattttt aatatgtatc atcgagcgtg ggaatatgcc
     2281 agtgtgagtg aattgatttt aattgttaaa gctgtgacga catctatcgt tattacgatg
     2341 gtggtcgtga caattgttac aggcaataga ccgtttttta gattgtattt aattacttgg
     2401 atgatgcact tgattttaat aggtggctca aggttatttt ggcgtattta tcggaaatac
     2461 cttggaggta agtcatttaa taagaagcca actttagttg ttggtgctgg tcaagcaggt
     2521 tcaatgctga ttagacaaat gttgaaaagt gacgaaatga aacttgaacc ggtattagca
     2581 gtcgatgatg acgaacataa acgcaatatc acaattactg agggtgtaaa agtccaaggt
     2641 aaaattgcgg atattccaga actagtgagg aaatataaga ttaaaaaaat catcattgca
     2701 attccaacta ttggtcaaga gcgtttgaaa gaaattaata atatttgcca tatggatggc
     2761 gttgagttat tgaaaatgcc aaatatagaa gacgtcatgt ctggtgagtt agaagtgaac
     2821 caacttaaaa aagttgaagt agaagattta ctaggcagag atcctgttga attagatatg
     2881 gatatgatat caaatgaatt gacgaataaa actattttag ttacgggtgc aggtggttca
     2941 ataggatcag aaatttgtag acaagtttgt aatttctatc cagaacgtat tattctactt
     3001 ggccatggtg aaaacagtat ttatttaatc aatcgtgaat tgcgaaatcg cttcggaaaa
     3061 aaatgttgat atcgttccta ttatagcgga tgtgcaaaat agagcgcgta tgtttgaaat
     3121 tatggaaacg tataaaccat acgcagttta tcatgcagca gcacacaagc acgtgccgtt
     3181 aatggaagac aaccctgaag aagcagtacg taataatatt ttaggtacga aaaatactgc
     3241 tgaagctgct aaaaatgcag aggtaaagaa attcgttatg atttctacgg ataaagccgt
     3301 taatccgcct aatgtcatgg gagcttcaaa gcgaattgca gaaatgatta ttcaaagttt
     3361 aaatgatgaa acgcatcgaa caaattttgt tgcagtgaga tttggtaatg tacttggatc
     3421 gagaggatct gtgattccac ttttcaaaag tcaaattgaa gaaggtgggc cagttactgt
     3481 gacacatcct gaaatgacac gttactttat gacaattcct gaagcttcta gactagtttt
     3541 gcaggcaggg gcattagcag aaggtggcga agtatttgtg ctagatatgg gagaaccagt
     3601 gaaaattgta gatttggcac gtaatttaat taagctaagt ggtaaaaaag aagacgacat
     3661 acgcattact tatacaggga ttagacccgg cgaaaaaatg tttgaagagc ttatgaataa
     3721 agatgaggtt catcctgaac aagtatttga aaaaatttat cgtggcaaag tacaacatat
     3781 gaaatgtaat gaagttgaag cgattattca agacatcgtc aatgacttta gtaaagaaaa
     3841 aattattaac tatgccaatg gcaaaaaggg agataattat gttcgatgac aaaattttat
     3901 taattactgg gggcacagga tcattcggta atgctgttat gaaacagttt ttagattcta
     3961 atattaaaga aattcgtatt ttttcacgcg atgagaaaaa acaagatgac attcgaaaaa
     4021 aatataataa ttcaaaatta aagttctaca ttggtgatgt gcgtgatagt caaagtgtag
     4081 aaacagcaat gcgagatgtt tattacgtat tccatgcagc agctttaaaa caagtgccgt
     4141 catgtgaatt ctttccagtt gaggcagtga agacaaatat tattggtaca gaaaatgtct
     4201 tacaaagtgc tattcatcaa aatgttaaaa aagtcatatg tttatctaca gataaggcag
     4261 cgtatcctat taatgctatg ggtatttcaa aagcaatgat ggaaaaagta ttcgtagcca
     4321 aatcaagaaa tattcgtagt gaacaaacgc ttatttgtgg tacaagatac ggtaatgtga
     4381 tggcttcaag aggatcagta atacctttgt ttatcgacaa aatcaaagct ggagaacctt
     4441 taacgattac agatcctgat atgacaagat ttttaatgag cttagaagat gcggtagaac
     4501 tagttgttca tgcatttaag catgcagaga caggagatat tatggttcaa aaagcaccaa
     4561 gctcaacggt aggggatctt gcgaccgcat tattagaatt gtttgaagct gataatgcaa
     4621 ttgaaatcat tggtacgcga catggagaga aaaaagcaga aacattgttg acgagagaag
     4681 aatacgcaca atgtgaagat atgggtgatt attttagagt gccggcagac tccagagatt
     4741 taaattatag taattatgtt gaaaccggta acgaaaagat tacgcaatct tatgaatata
     4801 actccgataa tacacatatt ttaacggtgg aagagataaa agaaaaactt ttaacactag
     4861 aatatgttag aaacgaattg aatgattata aagcttcaat gagataggag agattgacgt
     4921 tgaatattgt aattacagga gcaaaaggtt ttgtaggaaa aaacttgaaa gcagatttaa
     4981 cttcaacgac agatcatcat attttcgaag tacatcgaca aactaaagag gaagaattag
     5041 agtcagcatt gttgaaagca gactttgtcg tgcatttagc gggtgttaat cgacctgaac
     5101 atgacaaaga attcagctta ggaaacgtga gttatttaga tcatgtactt gatatattaa
     5161 ctagaaatac gaaaaagcca gcgatattat tatcgtcttc aatacaagca acacaagata
     5221 atccttatgg tgagagtaag ttgcaagggg aacagctatt aagagagtat gccgaagagt
     5281 atggcaatac ggtttatatt tatcgctggc caaatttatt cggcaagtgg tgtaagccga
     5341 attataactc agtgatagca acattttgtt acaaaattgc acgtaacgaa gagattcaag
     5401 ttaatgatcg gaatgttgaa ctaacgctaa actacgtgga tgatatcgtc gctgaaataa
     5461 agcgtgctat tgaaggaact ccaacgattg aaaatggtgt acctacagta ccaaacgtat
     5521 ttaaagtgac attgggagaa attgtagatt tattatacaa gttcaaacag tcacgtctcg
     5581 atcgaacatt gccgaaatta gataacttgt ttgaaaaaga tttgtatagt acgtatttaa
     5641 gctatctacc tagtacagac tttagttatc ccttacttat gaatgtggat gataggggtt
     5701 cttttacaga atttataaaa acaccggatc gtggtcaagt ttctgtaaat atttctaaac
     5761 caggtattac taaaggtaat cactggcatc atactaaaaa cgaaaaattt ctagtcgtat
     5821 caggtaaagg ggtaattcgt tttagacatg ttaatgatga tgaaatcatt gaatattatg
     5881 tttctggcga caaattagaa gttgtagaca taccagtagg atacacacat aatattgaaa
     5941 atttaggcga cacagatatg gtaactatta tgtgggtgaa tgaaatgttt gatccaaatc
     6001 agccagatac gtatttcttg gaggtatagc gcatggaaaa actgaaatta atgacaatag
     6061 ttggtacaag gcctgaaatc attcgtttat catcaacgat taaagcatgt gatcaatatt
     6121 ttaatcagat attagtacac actggtcaaa attatgatta tacattgaat caaattttct
     6181 ttgatgattt ggaattaaga caaccggacc actacttaga ggcagttgga agtaaccttg
     6241 gagaaacgat ggggaatatt attgcgaaga catatgatgt tttattacgc gaacaaccag
     6301 atgcactttt aattcttggt gatacaaata gttgtttagc agcagtatct gctaaacgat
     6361 taaagattcc tgtgttccac atggaagcgg gtaatagatg ctttgatcag aatgtacctg
     6421 aagaaatcaa tcgtaaaatt gttgaccatg tcagtgatgt gaatctacct tatacggaac
     6481 atagcagacg ttatttatta gatgaaggcc tcaataaagc gaatatcttt gtgacaggat
     6541 caccgatgac agaagtgata gaagcgcatc gagataaaat taatcacagt gacgttttaa
     6601 ataaactagg attagaaccg caacaataca ttttagtatc tgcgcataga gaagagaata
     6661 tcgataatga aaagaatttt aaatcattaa tgaatgcgat aaatgatatt gccaaaaagt
     6721 ataaaatgcc tgtgatttat tcaacgcatc caagaagttg gaagaaaatt gaagaaagta
     6781 aatttgaatt tgatccatta gttaaacagt taaagccatt tggtttcttt gattataatg
     6841 cattgcaaaa agatgcattt gttgtgctat cagatagtgg aacattgtca gaagagtcgt
     6901 ctattttgaa gttccctggt gtccttattc gaacttccac agaaagaccg gaagtactag
     6961 ataaaggtac ggttattgta ggtggtatta cctataacaa tctaatccaa tccgttgaac
     7021 tagcaagaga gatgcaaaac aataacgaac cgatgattga tgctattgat tataaagaca
     7081 ctaacgtttc gacaaaggta gttaaaatta ttcaaagcta taaagatatt atcaatcgaa
     7141 atacttggag gaaatgacga tgaggatagc gattgaaaag ataattggtt tgctgaaaaa
     7201 ccagtcctct aaagaatcga atgttaagat tcatcgcttg gcgtatatta caaactcaaa
     7261 atttgatggc aataactata tagatagatg gtgtaaaatc aggaattctc acattggtga
     7321 atacagttat attggatttg gtagtgattt taataatgta gaagtaggaa gatattgttc
     7381 gatatcttcg gatgtaaaaa ttgggttagg aaaacatcct acacactttt ttagctcatc
     7441 accgattttt tattctaata ataatccatt taacataaag caaaagttta tagactttaa
     7501 tgaccaacca agccgtacaa caattaaaaa tgatgtgtgg attggtgcaa atgtaattat
     7561 tatggatggt ttaacaataa atactggtgc agtcatagca gccggctcag ttgttactaa
     7621 aaatgtagga gcatatgagg ttgttggtgg tgttcctgca aaagtgatta agaagcgatt
     7681 tgacaataaa acaattgaaa aacttttgga aagcaagtgg tgggagaaaa cgcctgacaa
     7741 actaaaagga ttttcggttg aatatttaaa taaaaaggat acttaatgat atgagaattt
     7801 taaatattgt atcgagtaat attgttcaag acccaagggt acttaaacaa atagaaacaa
     7861 ttaaaggcgt tacggatgat tataaaattg ttggaatgaa taattcacaa gctactaata
     7921 agcgattgga aaatttagat tgtaattatc gtttgttagg tagcaaggta gatccaaaaa
     7981 atattctttc taaattaatt aagcgtataa gatttgcaac aggtgttatc cgagaaatta
     8041 aagcttataa acctgacgtg attcatgcaa atgatttcga cgtattatta atggtctatt
     8101 taagcaatta taaaaaagct aatattgttt atgatgcgca tgaaatatat gcgaaaaatg
     8161 cctttattaa taaagttcca cttatttcaa agtttgtaga aagtatagaa aaacacatag
     8221 taaaacatcg tgttaatgcc ttcgtaacag taagtcatgc agcaaaagaa tattatcaat
     8281 ctaaaggata taagaaggaa gcgaatgtta ttacgaatgc acctatttta aatgatagca
     8341 gagaatttaa agaaatcgaa aactttaaag aaattgtata tcaaggtcaa attgtaatgg
     8401 acagaggata tgaagagttt attattgctt catcagcttt taaacaaaat gctccttcat
     8461 tcataattcg agggtttggt ccgcatgaag aagtgataaa agaactgatt agttataacc
     8521 cggaaaatat taggttggat aaaccagttg aagtaaaaga attggttgat aagttagcag
     8581 aaagtaatgt tggtgttgtc ttgacgaaac cagtatctat taattttgaa tatacagtat
     8641 ctaataaaat ttttgaatgt atacatgctg gtttaccagt aattttatct cctgtcaaag
     8701 agcatattta tctcaatgaa aaatataaat ttggcattgt tctaaaggaa gttacgccgt
     8761 tagaaattga aaaggcggtt agaaaattaa gagataatca cgatttgttt aatcatttac
     8821 gtcaaaatgc aattaaggcg tctaaaattt tgaattggca aatagagagt gaacggttag
     8881 tagaattata taaattttaa agagaggtag actatgaaat tttttgtact ttgtgcaatt
     8941 atcagcatga acatatttat agtaatctct acatttacta aagaagtatt agggttccct
     9001 atagagccgg tgtattactc aaccatggtt ggtatagcat taattactac ggtgtttgct
     9061 atttataaga taattgtcac ccaagaaatt ccgcgagggt taatattatt aattgctata
     9121 tgtttgcttt atctagcttt ttattatttt tcaccagata aggaagagaa actagctaaa
     9181 aataatattc tattcttttt aacatgggca gttccagcgg caattagtgg tatttatatt
     9241 aaatatataa acaaggctac ggtagaaaga ttttttaaat tagtattttt catattttct
     9301 gtttcattta tttttgtaat tttaatacca aaacttacag gtgagatacc tagctatatc
     9361 aattttggac ttatgaacta tcaaaacgct tcgtaccttt cagcatttac tgccggatta
     9421 ggcatttatt tcattatgaa aggttcagtt aaacataagt ggatatatgt tctatttaca
     9481 ataattgata tccctattgt gtttatacca ggagggcgtg gaggtgctat tttattaatt
     9541 ctttacggct tatttgcatt tatacttatt acgtttaaaa gaggaatacc tatcgcagta
     9601 aaaagcatta tgtatatttt tgcattaagc atatctagtg tattgattta ctttcttttt
     9661 acaaaaggtt cgaatactag aacattttca tatctacaag gtggaacact taatttagaa
     9721 ggtacttctg gaagaggacc gatttatgaa aaaggtattt actttattca acaaagttcg
     9781 ttattaggct atgggccatt taactattat aaactaatcg gaaatatacc acataacatc
     9841 attattgagt tgattctatc atttggctta ttagggtttt ttatcataat gatttgcatt
     9901 ttgctactag tttataaaat gattaggaac tatgatccaa acactataga tttactcgtt
     9961 atgtttatag caatctatcc aatcacatta ttaatgttta gttcaaatta tttagttgta
    10021 agtgaatttt ggtttgtgtt gttctatttt attacaaaag gacggcgtca tcatggctaa
    10081 gaaagttttt attatggata gcgtaaagac aataattggt acgttgctta tagctttagg
    10141 attacaattt ttagcttatc caattattaa tcaacgagta ggtaatgaag cgttcggttc
    10201 tattttaacg atttatacaa taataacaat cacgagtgtt gtattaggca atacgcttaa
    10261 caatatacga ttgattaata tgaatctata caaatccaat cattactact ggaaatttgc
    10321 atcgatactt ttaatctcaa ttctgattga gagtatagct ttaattattg tatttcttta
    10381 cttttttaa
//
LOCUS       DALAGF010000091        10292 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.091, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000091 DALAGF010000000
VERSION     DALAGF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10292
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.091"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(900..1094)
                     /locus_tag="O2692_002139"
     CDS             complement(900..1094)
                     /locus_tag="O2692_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299757.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYC
                     LLPLIVNLSIYGFYVFVKRK"
     gene            1204..1896
                     /locus_tag="O2692_002140"
     CDS             1204..1896
                     /locus_tag="O2692_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299758.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKS
                     KNIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEAN
                     DKNAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKN
                     YVEYKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAV
                     CGTAFAAGCSVG"
     gene            complement(2064..2213)
                     /locus_tag="O2692_002141"
     CDS             complement(2064..2213)
                     /locus_tag="O2692_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299759.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIV
                     FYEDK"
     gene            complement(2760..3076)
                     /locus_tag="O2692_002142"
                     /pseudo
     CDS             complement(2760..3076)
                     /locus_tag="O2692_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            complement(3069..4029)
                     /locus_tag="O2692_002143"
                     /pseudo
     CDS             complement(3069..4029)
                     /locus_tag="O2692_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181110.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            4444..5187
                     /locus_tag="O2692_002144"
     CDS             4444..5187
                     /locus_tag="O2692_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCZ4299760.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            complement(5414..5599)
                     /locus_tag="O2692_002145"
     CDS             complement(5414..5599)
                     /locus_tag="O2692_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4299761.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(5604..5942)
                     /locus_tag="O2692_002146"
     CDS             complement(5604..5942)
                     /locus_tag="O2692_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299762.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            complement(6188..6796)
                     /locus_tag="O2692_002147"
     CDS             complement(6188..6796)
                     /locus_tag="O2692_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ4299763.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            complement(7282..8181)
                     /locus_tag="O2692_002148"
     CDS             complement(7282..8181)
                     /locus_tag="O2692_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HCZ4299764.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            complement(8280..9746)
                     /gene="msr(A)"
                     /locus_tag="O2692_002149"
     CDS             complement(8280..9746)
                     /gene="msr(A)"
                     /locus_tag="O2692_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCZ4299765.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
     gene            10220..>10292
                     /locus_tag="O2692_002150"
     CDS             10220..>10292
                     /locus_tag="O2692_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCZ4299766.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRY"
BASE COUNT         3497 a         1584 c         1439 g         3772 t
ORIGIN      
        1 ataaatgcgt gtctatgcgt agacatggag acacttcaga attatggagt gattacatga
       61 gtttaactat ttttctttat tcagttttag gaagtttatt agcaaattat atctacgaaa
      121 aatcttcaaa ataaataact ttttcggttc tactttgtac actttatcta cctggtagac
      181 ctaattgtta ttgctttaca ggtctaaaat tcgtcgttat aatctagtta aatcccgtaa
      241 gggcgcactt atacgtcgga taaatcgaca tgtgcaaaaa cgtaatcgac cttcgtcgat
      301 ttttttattt caaaattaaa gaaacggctt gagatattcc gagcagttat aaaaattaat
      361 ttgcgttgaa tattgctact acattcttga aaaagtgatg tagatttata gcccataact
      421 tttttgaatt acatcgacga tacggcgtcg aaagaaaaat aaatagaccg tatgaaagaa
      481 aaagctacct cactttatct ggggtagctt ttatttttta tgcccttatt ttgaatttta
      541 agagggcatt ttaaagattt aggggtgatt catatagttt tatatctaaa acttatatat
      601 gcttttaaaa cgcaaatatg agccaaataa atatatgcta ttttcaaaaa caaaaatttg
      661 agcaaaaaca ataactattt ttagacactg cccatttaca tgcaaattaa aaattagcat
      721 aaaattcttt aaaacttccc cacgtttttc taaattcctg tagggcgttt ttatcaaaaa
      781 tcaagacaag aagaaactcg ttcacactcg tttcaaaaaa acaagaaatc taatctatac
      841 acctaccgtt ttccaacccc ctcaaagcat acaaattaaa aacaaaatta ttcataatct
      901 tactttcttt taacaaaaac ataaaaacca taaatagaca aattaactat caaaggcaat
      961 aaacagtata aaaaaaccac gaataaatct tcatcaacat ctaaaataaa aaagaataaa
     1021 acaattaaca aaatactaat taacaaacct aaaagtggca aactttttat atatttttct
     1081 aaattttcat tcataaaata gactcctttc aaaaatatgt ttataaatat attaaaataa
     1141 atattgattt tataaagcgc ttacttatac aattaaaata tcttattatc ttggaggtat
     1201 caaatgaaaa ttataaaatt attttcaata atttccttgt tcctactggt atttagcgcg
     1261 aatttttcta atgcttacgc aaatgaagaa caaaaaagtt ctttactaga aaatcaaaaa
     1321 gaaaaattga aaagcaaaaa catcgaagta tcacaaatga ccaagaaaga aataagtttt
     1381 ctagaacaat tgcaaaataa aaatgaagat atgcaaaaat tcgaaaagca aatgaaaaaa
     1441 gaaggattta aaaaaataaa gctagaagac aaaaattcta aattaaaata tctagctaca
     1501 gaagcaaatg ataaaaatgc atatggctac atagctaata acgcttataa aaatgaaaaa
     1561 acaaaagaaa ttgtagtttc tgaaacaata tatgataact acaacaaaaa aatcacaaaa
     1621 ttcattgccg aaaaaagaag tatgactgaa aacaaagagg gaaaaatact tattaacaaa
     1681 aattacgttg aatataaggc tgaaaaagtt gataaaaaca gaccggtaac atatgcagga
     1741 ttcaaatgga acggcaaagc attcgcttgt agtatggctg gactttatgc atgtgctcac
     1801 tactgtggag tatgggcaat cgtaaatcca atagccggcg gtgtatgtga agcagtttgc
     1861 ggaactgcat ttgcagctgg atgctctgta ggttgatttt attccaattg ttttattgac
     1921 gttgagcctc ggaaccctta acaatcccaa aacttgtcga atggtcggct taatagctca
     1981 cgctatgccg acattcgtct gcaagtttag ttaagggttc ttctcaacat caataaattt
     2041 tttcggcata aatacgatgt tccttattta tcctcataaa aaacaatagc tgaaatatat
     2101 aaccaattac tgtgtgtttg aatttctaat acatgaatat cattctttaa aaacctatta
     2161 acttgtttat ctaattgacg atgagtaaaa gtagcgaaag ttttaatctt catgatatct
     2221 ctccttttat aaaaatagca atccttatta caatattaaa cacttttatt ttggatttta
     2281 agaggcattt tgaagactta gaggcaaatc atataggttt tgtacttaaa aatctataca
     2341 gtctcttaaa acgcaaatat gagccaaata aatatatgct aatttcacaa acaaaaattt
     2401 cagcaaaatc agtgactaat tttttagata ctgcccattt acatgcaaat ttaaaaatta
     2461 gcataaaata ctttaaaact tccccatgtt tttctaaatt cctgcagggc ttttcttatc
     2521 aaaaacttag aaattttaat ttaactctaa aatctccgta agagcataag ggaaatttgc
     2581 tatcattcaa aaatgataga tctggcaagg ccagacattc caagggtttt aagttatttt
     2641 aaagtgatat catacaaagc tgtagtgtca cacttttgtg acactacata cagcatttgt
     2701 cacaaaaata agacagcact tttttacaga aaaacaataa aaaaagacat cagttatcac
     2761 taatgtctta aataataatt ttgcttttcg aaattccaaa ttgcggtaat tgaatgtgct
     2821 ttttttcttc gtctgtcgtt ttatcttcat cacttgtatt aatcaaatta ccatcttcgg
     2881 catcgtctaa gtttaaaatt ttatgctttt gtttaagcaa tccaccataa cttaacatac
     2941 gttttcgata caaacctttt tctaaatcac ttacaatttc ttgatttttt tcgtcgtcat
     3001 cagttaaaaa gtcagatgat ttaacggaat attttgatgt ctcttcgagt gttataaaat
     3061 catttgaatt attgattaaa gatattatat tgtttcatgt ttttacggcg catataaagg
     3121 gcatgtgttt cttcaagttc agttagaatt gtttgcatta aataagcttg cattgacttg
     3181 attgtttcct tttggccttt aactccccta tcatgtatag cttttttaag tctttttaaa
     3241 gaagttaata tttcatcctc aatgtcctct aacatgtaat gcatatcttc atgccttgaa
     3301 ttaaatgatt tttttgcttt aagaataaca gcctttatga tttctaaatc tctataatca
     3361 tattttaata aataattagt tagttgttct gggaattctt gcaatagcat ttctttatca
     3421 tcaaaattat gtgtaaattg atttgaatga tttgaatgat tactaatttc atttttgtac
     3481 tttatatcat tcaagtcatt catatcatta ttctcagtat cattaaagtc aatgtcatta
     3541 aagtcagtat cattagtgtt cgattttcga acttctggac gttcgatttt cgaacttctg
     3601 gacgttcgat tttcgaactt ccttctcgcc gtaaggttca acatcgtttt cttctttttc
     3661 tattttgtaa atatcacgtt ctgtgacaat aggtttcatt aaatatagaa tatttggttt
     3721 atttaaacca cgtcgttttt gaatgagtag accatcgttt tctaaccctt tttttatttt
     3781 tgttaacttt tcttttttac aatttaagat ttccattagc ttttcatttg aataaacaaa
     3841 gtaaatgtta ccatcttcat caacccagct atttttaata gataaattta agcgatcacg
     3901 taagatagca taggctattt tcatatcatt agttagattt ttataatttt cactggtaaa
     3961 gaatacttta gggatttgat aaaatctttc tttgtaattt tcttgcactg taaaatattg
     4021 attttgcatg acaaaaaacc tctttccata ttttagtttg gtaagaggta ataaaacgtt
     4081 tttatttagc tttacattta tttaatgatt taggtgcttg cactaagata ttattcatta
     4141 tataatacaa gtatcgaaaa ttatataaat aaacagtttc taaacgtttt attaccaaac
     4201 cccgactatt accagtagtt ggggtttttg ttatattaag tttattatag cactttaaat
     4261 aaacttataa aagggaaaat atagctttat caagggttta taaacccttg ataaatatca
     4321 taaacccttg ataaaaaggt tgatggatgt ttatttttat ttattaatca ctttattatt
     4381 aagcattata agcttataat attttatcaa gggtttataa acattttata aagggttgat
     4441 gatatgaaaa gtgtgaaaaa gttgagtgaa gaattagggg ttagtaagca aacgattttt
     4501 aataatatta agcgattgaa tatagagaca ataaaacagg aaaacacttc gtttattaaa
     4561 gaagataccg atatagaaaa aatcattcaa agagtaaatg aaaataaaaa gaaatatggt
     4621 tttgaaagta ctactgaaga caaacaaaaa aaagggtcgg ataatattaa ttttgaatcg
     4681 aaaagcgatg ctcaaatagt agaaatcctt aaaaatcaga ttgatacttt aaataatcaa
     4741 attgaaaaac aagaaagtcg tcatgaaaca acaattgaat tttacagaaa agagttgcaa
     4801 gaaagatcaa aattgcttga aaatcagcaa gttctggcat tagagagtaa taaaaagatt
     4861 caaaaattag aaaatcaatt agaaaaagaa aaacacctta attatccctt tgatacatct
     4921 actaacacta gacaaaacgt tgatgcacaa gaaaaaacat atactactaa tcctgtaaat
     4981 attaacagag atcaggaaga aactaaagaa acagagattc aatataagga tatatctgga
     5041 agtcaaagcg acgaaagtac acaaggtgaa gaagcaaaaa aagaagacgt tccagctaat
     5101 ctaaatgaca atgatagtga tatagaagaa aagagtgaag aaacggaacc taaaaagggg
     5161 ttttggagtc gtttgtttgg tagctaaaat aattgattag gcactaaaag ttataaaatt
     5221 ttagtgtctt ttatatagtg agaacttctg gtaatcatcg attatgtaaa ctaaagtatt
     5281 tcaaataatt aactgtatta atactctcat catgattgcg ttataaacgt tcgttattga
     5341 gacaaaattt aatagtacta ctacacaaaa agagagtgcg aattcgcact ctctttttgt
     5401 gataaatgaa tggttaagaa aataattcat ccacagttgt atcaaacaat cttgccaact
     5461 taaatgctaa tgtcaatgta ggatcatatt tattgttttc tattgcatta atagtttgcc
     5521 tagacacttt taattcttta cttaaagttt cttgagaaat tcctttttgt tttctatatt
     5581 ctctaattct atttttcatt aatttaacct ttagatgtat attttttatt tgtagtatag
     5641 taattaaaca gcataagaaa tagtgctaca attataaaga aaatttctgg atattgagaa
     5701 aaccaagatc cttcaatact tggtgacaaa cctgtaaatt taagcactag tgtaaataac
     5761 atacaatata caatccaaat aaaactttta gccagtattt caataaacat tttacgttgt
     5821 ctttcatcgt atcgatcttg ttgtcctttt ttatctaatg tttctgaagg taaatatttg
     5881 ttagttatag ccgtccataa acaagaaaaa attaaaataa taataaaagg aattaaattc
     5941 attataacac ctcataaatg taaaaagtat ttgacattta tgagtgtaaa tatttttgat
     6001 acaaatgtca aatacttttt acattataat atattttaaa acaccttatt agtaaaacaa
     6061 ttactatata gaaagctgcg aactcgtact cttttttctt gtataattat taaaatctca
     6121 attcaataat atgttatgga atttgagaca ctgtgagaga cgctcatttg cattgacaat
     6181 caatttatca agataatccc ttatcatgtt taattctata aactgtttgt cttgtgatat
     6241 taacttcttt cgcaatctta ctaatcgctt tgccttcttc taacatttcc accacgcgat
     6301 gataaataac acgtttctgt gggtctttag catttggtga ataaagtaaa gggcgccctt
     6361 tatatacgcc tttttccttc gcaacttgaa tcccttgcgc ttgtcgacgt ttactttcat
     6421 ttctctcttg ttctgaaacc attgctaata tttgaatgat taaatctttc ataaacttat
     6481 ccagtaatgg attgccaatt acttcattca tcataggtaa gctggtaatc attaactgaa
     6541 cgtctttttc ttttaaataa ttcacagttt caataatttc atcatagtta cgtcctaaac
     6601 gatcaataga ttccacaacg aatcgatctc ccattctgac aaaattgagt gcttcttgaa
     6661 atacaggtct attttccact gactttcccg actgtttctc tgtaaagatt ttctccacac
     6721 caaacgtctt taaattatcc aattgtcttt ctaaattttg atctatcgat gatactctcg
     6781 cataacctat aatcattcat cttcactcca taataaatat acaccctaat catacgtttt
     6841 aattgttata atttcaacaa ttctttttgt atggttaggg tgtactcaaa gtgtacatat
     6901 ctataattaa aacttactaa aaataaatat agattttttc tttgtaacag aaccgttcat
     6961 ttgaaggtca tctctatcat aaatatttaa attaactaaa aattttaatc atctgtttaa
     7021 tacttatgaa atgtatttac ggtaggtatt aaaattgatg aaatttatat aagtgctttc
     7081 aatgaaaatg accgtttatt tatcctcctg atttaaggag ttagaaattt cgaaaacgat
     7141 tctttccatt tgttgcattt gttccatgtc cagatattta atacattata gaaaaccaaa
     7201 gaccatcact aattaaacgc agttttaaaa tttcatcagg attgccttcg tctttaaaat
     7261 gggcctcttt taatataagt tctactcttt catacctaac tctttcaatg ccatcgtttc
     7321 atacgccgat tctcctgatt ccatagcaaa ctcagcgata aacattggga atactgcatt
     7381 gagttctatt atatgctctt tcattcgtgg ccatatttca cctccagctt taccgtatgc
     7441 tgttataagt tgctctaatc cttcgtcgtc gaatacacga tggtgtccta taaagtccat
     7501 tgatgggtcg gagtgggttg cttcagtcca gtctatgaga cctgtgacgt ttgcttggtt
     7561 atctaccatt atatgtcctg gatgtaagtc cccatgtatc atggtcgcat gtcgaggcca
     7621 tagttcgtcg ttttctaacc attgtttcca tctgttccat aattcatctg atacgccata
     7681 agtttcttta actttattca ttcttctttg aaagtcattt tttatttctt gtatggtttt
     7741 gatatttata tgctgaacgt taatgttttc ttctggtatg ttgtgtaaat ctacgagtgt
     7801 ttcagctaat gtgttaataa agttttctgg taatggtttg tgttcaattt cccatacata
     7861 attttgtatt tctggatcta ttgtggctgc gggtttacct gtaagttttg ggtaagcaat
     7921 aaggtctttt gcatgtactt tccacttcgg tatttcaaat gaaacattct tctgtaagaa
     7981 gtctaccgtt tgtttttcgg gttttgttcg tttataaacg tcaggtcttc ttggtagtct
     8041 caaaacccat tctactccat gtttatcttt tccaaatgca acttgaaaat caagtcccga
     8101 ttcattcaat gagattgttt gaggttggat gtgtaattga tatttttctg cacatttaat
     8161 aatttcatta tgtcgagtca ttttattgga tccttatttc tattatattt ttcttgtgaa
     8221 cgtatttttt atattgatat aaatgaacct aatattcaat ataaattagt gtatagtttt
     8281 taagttatat catgaagaga ttgtcctgtt aattcccatt ttttatctga tacatgttta
     8341 acaaaccttg tatcatgtga tgtaaacaaa atgattccag gatatttatt cataaacatt
     8401 tctaatgctt ctaatgtttt aatatctaaa aaattagttg gttcatccaa aattaacata
     8461 ttcgctttcg ttgaaaataa tactgctaac gataatttcg ttctttcccc accactcaaa
     8521 acattacaag aacgatcaag tgcttcattt aaacccaagt tatttaaaat agctctactg
     8581 aatgattctg atgaatccgt ttcatccatt aaatattgca ataatgaaac gtcacgcatg
     8641 tcttcataag caagttgacg atagtatgcc atttgcactt taggagaaca atcaattccc
     8701 tctatttggt ggtaaatagc ttcaagtaaa gttgtcttac ctacaccatt tgcacctacg
     8761 agcgctatat ttttgccata tggtatttgg aatcgtactt gtgttagcag tttttgactt
     8821 cctttaacca atgttagatt ttgtgcaatg attggataat tattatggat atcataaatt
     8881 ttattttgtg ggaaattgaa ttcatgataa ctttgtggtt tttcaacttc ttccaaatgt
     8941 tccattcttt tttcaatatg cttagcttgt ttttgagcag ccttctcaac cgtgcctttt
     9001 tgttttgatg cacttaaacg atctggtgct atacttttgt ttttttgttt tgatgatgct
     9061 tgtgccattt gttgcgcttg atttcgttta gctttactgg cttgggacaa tctttgtttt
     9121 tcacttatat actgttcata ttgacgttgt tgttctaact gttcttgttc atattgattt
     9181 tgatactgtg tgtaattacc tttaaacact cttattgtgc catcttcttg tatatcccaa
     9241 attgtgtcag caatttggtc tataagtgct ctatcatgac ttactataat taaagtacca
     9301 tagtaatatt ttaaaatatt tttcagataa tctttaccaa ttttatccaa gtgatttgta
     9361 ggttcatcta aaagtaatat cggactataa tttgatatga cattagctaa tttatatttt
     9421 gctttttcac caccactcat actgtcggtt gtatgcatag gtatatggag ttcactcatt
     9481 aaactaccgt ctaacgtgtt aaaatcattt tccacatcca tgttaagctg ttcaaaatat
     9541 tgaatttcgc caaccgtttc aactgttcca gattctggtt ttgttttttg agcaatcata
     9601 tttagtaatg tggttttgcc agtaccatta cccccaataa gtgctatttt ttcaaattgg
     9661 taagcgtaaa gatgaccgat gttaagtgat cgtaaatctg tcaatttatg attgatttgg
     9721 ttaaatttaa ttgtatattg ttccatattt atacactcct tgtatattta aaggacgtgt
     9781 acgcaaaaaa atgagacagg ctactcccgt ctcattcaca ttacactttc aaacacttaa
     9841 aaatttatta tgatgtttta gtatttgaaa gcgtttatta ttcctatcga aggataaaaa
     9901 atgacataag tcatcctatg agtgtgtatt gcggacaagc ctttttgaat taatttagtt
     9961 ttgtattcaa aaaggtttat ttgagtctca tagaagctgt cattttaatc ctccttcatc
    10021 gataaaatag taatgttaat ataacatttt gttggttttt ttacaatata tctctaagta
    10081 gaaagattat ataacaaaag tataaaaata caattttaga ttgattatac atctccttaa
    10141 tttagagtgt actcataaaa tacggttctg ttgcaaagtt gaatttataa tataatttta
    10201 acaaaaagga gtcttctgta tgaactattt cagatataaa caatttaaca aggatgttat
    10261 cactgtagcc gttggctact atctaagata tg
//
LOCUS       DALAGF010000092        10142 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.092, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000092 DALAGF010000000
VERSION     DALAGF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10142
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.092"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(14..841)
                     /locus_tag="O2692_002151"
     CDS             complement(14..841)
                     /locus_tag="O2692_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4299767.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
     gene            complement(844..963)
                     /locus_tag="O2692_002152"
     CDS             complement(844..963)
                     /locus_tag="O2692_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299768.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            1075..2076
                     /locus_tag="O2692_002153"
     CDS             1075..2076
                     /locus_tag="O2692_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HCZ4299769.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            complement(2198..2662)
                     /locus_tag="O2692_002154"
     CDS             complement(2198..2662)
                     /locus_tag="O2692_002154"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCZ4299770.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(2675..3856)
                     /gene="ribB"
                     /locus_tag="O2692_002155"
     CDS             complement(2675..3856)
                     /gene="ribB"
                     /locus_tag="O2692_002155"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCZ4299771.1"
                     /translation="MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            complement(3867..4499)
                     /locus_tag="O2692_002156"
     CDS             complement(3867..4499)
                     /locus_tag="O2692_002156"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCZ4299772.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            complement(4506..5549)
                     /gene="ribD"
                     /locus_tag="O2692_002157"
     CDS             complement(4506..5549)
                     /gene="ribD"
                     /locus_tag="O2692_002157"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCZ4299773.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     regulatory      complement(5676..5811)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(6030..7532)
                     /locus_tag="O2692_002158"
     CDS             complement(6030..7532)
                     /locus_tag="O2692_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCZ4299774.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSGQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECADYDI
                     DFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDI
                     YNVVINATGAKSHLNELDQDDQLIRNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ"
     gene            8055..8369
                     /locus_tag="O2692_002159"
     CDS             8055..8369
                     /locus_tag="O2692_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCZ4299775.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            8369..9661
                     /gene="arsB"
                     /locus_tag="O2692_002160"
     CDS             8369..9661
                     /gene="arsB"
                     /locus_tag="O2692_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HCZ4299776.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
     gene            9679..10074
                     /gene="arsC"
                     /locus_tag="O2692_002161"
     CDS             9679..10074
                     /gene="arsC"
                     /locus_tag="O2692_002161"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HCZ4299777.1"
                     /translation="MTKKTIYFICTGNSCRSQMAEGWAKQILADDWNVYSAGIETHGV
                     NPKAIEAMKEVGIDISNHTSDLIDNNIIKNSNLVVTLCSDADVNCPSLPTNVKKEHWG
                     FDDPAGKPWSEFQRVRDEIKIAIENFKSR"
BASE COUNT         3238 a         1791 c         1471 g         3642 t
ORIGIN      
        1 cctttttaga atattaaatt tctacaattt cgtcatcttc aacaataaag cccattgtat
       61 tgacgctgtt atttaagaaa gtcagaatat aacgcattac ttcatcacgt tctggctcat
      121 tgtgaacctc gtggtaaaaa ccttgccaag ctttaaaata taattcaggt gtttgatatt
      181 tttctttaaa ctcatcaatt gccctagtat caacaattaa atccttcgtt ccatacatta
      241 atagcgttgg cattggttga atgtcatgaa tatgagccat cgtatctttc atcgtctcat
      301 taattgtatt ataccaatga tacgttgctt tttttaacat taaaccatcg ttaactgttt
      361 cttcaacaat ttctaaatta cgtgttaaat cttttggttc tacaccaaca ttaaaacgtg
      421 tgtcttttga aattttacct atatttgaaa caagtttatc tttacgattt tttccattct
      481 tttgaagttc tagcatagga gaaattaaca tcatcccctc gattggcaat tctacttttt
      541 caagtaaatt taataaaatc aaaccgccaa gtcctacccc taatacataa gtaggaattt
      601 tatattcatt agctatcttt aaccagtcta gcaaactttc gtgatacgtt tgaaagtttt
      661 caatttgtcc tttattagct cttgaagttt gaccttgacc aggcaaatct cccataatca
      721 catgatagcc atttcttctt aacatcgtaa taacatatgc atatcttccc gtatgttcta
      781 atatattatg agcaataaca acgacgcctt tcgcatcatt ttcagcttcc cacttccaca
      841 ttattatact gccccttttt cattaatctt caataacata attatagcaa attcactatg
      901 tagatttcta tttatagtat tattgttgtc catattatta tatataaatg aaatcaacat
      961 caataatagt gtaattatac ataattattt ttgattgttt ttgatgaaaa cgctttctcg
     1021 aatatttttt tcatgctaaa cttattgtaa acacaagggt ttggaggagt agcaatggca
     1081 ctattaaaga atttttttat cggattatct aataatagtt ttttaaacaa cgcagcaaaa
     1141 aaagtgggcc cacgtttggg cgccaataaa gtcgttgccg gaaatacaat tccagagtta
     1201 attaatacaa tcgaatactt aaatgacaag aatatcgctg ttacggtaga caatttaggg
     1261 gaatttgtcg gtacagttga agaaagtaat catgctaaag aacaaatttt aacaattatg
     1321 gacgcgcttc atcaacatgg cgtaaaggca catatgtctg ttaaattgag tcagttaggt
     1381 gcagaattcg acttagaatt agcttaccaa aatttaagag agattttact taaagcaaat
     1441 acttacaaca atatgcatat aaatattgat actgaaaaat atgctagcct gcaacaaatt
     1501 gttcaagttt tagatcgctt aaaaggcgaa tttagaaatg ttggtactgt aattcaagca
     1561 tatttatacg atagccacga attagttgat aagtaccaag atttacgatt acgtttggtt
     1621 aaaggtgcat ataaagaaaa cgaatcaatt gcatttcaat ctaaggaaga cgtagatgca
     1681 aattacatca aaataattga acaacgtttg ttaaacgcac gcaatttcac ttcaattgca
     1741 acacatgacc atcgcatcat taatcatgta aaacaattta tgaaagaaaa tcacattgaa
     1801 aaagatcgta tggaattcca aatgctctat ggttttagat cagagttagc agaagaaatc
     1861 gcaaatgaag gctataattt cactatttat gtaccttatg gcgatgattg gtttgcgtat
     1921 tttatgagaa gattagcaga acgcccacaa aacctatctc ttgctgtaaa agaatttgtg
     1981 aaacctgctg gcttaaaacg tgttggcata attgcagctt taggagctac agttatgtta
     2041 ggtttaagta caattaaaaa attatgccgt aaatagagca agacataaac aataatttag
     2101 gagtctggaa caataatcaa tgttctaggc tcctaaatgt tatattggca gttctgaaac
     2161 atttataaat ctttgattat gaactcacaa cagtgctcta tgcttttata gattttaata
     2221 aattagccat ttcaattgca cttactgctg cttcagcacc tttattgcca gctttcgtac
     2281 ctgctctttc cacagcttgt tcaatacttt cagtcgttaa aataccaaat atgactggta
     2341 cattagtttg atcattcact ttagaaacac ctttcgcgac ttcattacaa acataatcat
     2401 aatgagacgt agcaccgcga attacgcatc ctaatgtaat tactgcatca taatttcctg
     2461 atgaggctaa ttttttagct actaaaggaa tttcaaacgc acctggcaca aatgctacat
     2521 caatattgtc ttcattaaca tcatgtcgaa tcaaagtatc ttttgcacct tcaagtaatc
     2581 ttccagtgat aaaatcatta aatcgactaa ctacgattgc aactttcaaa tcttttccaa
     2641 ttaatttacc ttcaaaattc atgttaaaat cctcctatat taaatgaccc atttttattt
     2701 ttttcgtttc catataatca tgattatgta ccgtttctgg tacgataact tcaattcttt
     2761 ctgcaatatc aatgccatat tgttttaatc cctcaaattt acttggatta ttacttaata
     2821 aattgatatg ttcgatgtta aaatatttta aaatctgtgc agcaatatga taatctcgca
     2881 aatcttcatc aaaacctaat gctaaatttg cagttactgt atcatatcct tgctcaatta
     2941 attcatatgc gcgtaatttg tttaacaatc ctatgccacg accttcttga ggtagataaa
     3001 taatcatgcc accatgttca ttgatatact tcatagacga ttcaagttga gcaccacaat
     3061 cacaacgttg actatggaaa atatcgcctg taaggcacgc agaatgtaag cgtacatttt
     3121 catgttgtcg aattgcacct tttgtcagta caactatctc ttcatctgtg tatgtcgctt
     3181 taaaaccata catatcaaat gttccgaaat ctgtaggcat tttcactttt gccttaaatt
     3241 caatttctgg ttctaatttt ttacgatatt caattaaatc atcaatcgta atcatcttta
     3301 attgatgttt ttctttaaac ttttgtaaat cttgtccttt cgccatcgtg ccgtcatcat
     3361 tcataatctc acaaatgaca ccagcgggct tggcaccagt aagtttagct aaatcaacag
     3421 ccgcttctgt gtgtccattt ctagctaata cgcctttatc ttgtgctact aatggaaata
     3481 aatgaccagg acgattaaaa tctttagctt cactactagg atcaatgagc tttttggcag
     3541 tcaatgtacg ttcataagca ctaattcctg ttgttgtatc tacatgatca atactcactg
     3601 taaattgcgt accaaagatg tcggagttat catcaaccat ttgtaccaaa tccaaacgtt
     3661 gtgcaatatc tttagacact ggtgcgcata ttaatcccct tgcttctttc gccataaaat
     3721 taatggtatt atcgttcatc cattcagtaa ccgctactaa atcaccttca ttttcacgat
     3781 tctcatcatc tactacaata attggttctc cattttttaa agccattaaa gcactgtcaa
     3841 tattatcgaa ttgcatgcta cccctcctaa aaaccaaatg ctcttaattt atctacagat
     3901 aattggtctt tatctttatt taaaatattt tcaacatatt taaacaaaac gtctgtttct
     3961 aaatgtactt tatctcctaa ttttttggat gataaaatcg ttgaacgcct cgtttctgga
     4021 ataagatgaa tgtcaaaact gttatcatgc ttatcaaata ccgttagact aacaccatcc
     4081 acagtaatag acccttgctt aactaactga ttattaatat gttggctaca ttgaatcgta
     4141 ataatttttg cattggctgt ttcatttatt tttgaaactg ttcctagttc atctacatga
     4201 ccgaggacaa aatgtccacc aaacctaccg ttaccactca tggcacgctc taaatttact
     4261 tctgattgtc gcttaacatc tgctaaatag gttttatttt cagtgccttt aattacttga
     4321 acagtaaaag atgtctgatt aaaatcaatc actgttaaac atgcaccatt aacactgatg
     4381 gaatcaccaa tatgcatatc tgccgtaatc ttatgtgctt caatttcaat cgtcctgact
     4441 gattgacgaa tttgaacact tttaacgaca cctatttctt caacgatgcc agtaaacatg
     4501 catcatcact tctttcgtaa agttaattta acattttgat ttaataactc ggaatgaaca
     4561 atttcaaatt ggttcgcatc tggtatctca atcacatcat ttgtttgata aaattgataa
     4621 tttccagatc cgccaattaa tttcggggca taatagagaa taaattcatc tatataatta
     4681 gattggagaa attctgaagt agtggttgga cctgcctcaa ctagcaaagt tccaactcct
     4741 cttttatata aattgtgaag aattgttgtt aaatcgcaag acttcaagta aataatttca
     4801 atatgtgttt gattggttgt taaatttgga ttttcagtat atatccaaat tggtgttgat
     4861 tcatcttgat aaatttgctg attaaaatga atattcccag acttagacaa tattactttt
     4921 atagggtttt ttccatcttg aatacgtgta gtatattgtg gatcatctaa ttcaactgta
     4981 cgtcttccag ttaacactgc gtcgtgtcga tgtcttaact tatagacatc ttgtttaacc
     5041 tctttgttag taatccattg actttgtcca ttatcattcg cttgtttacc atctaaactt
     5101 gcagatactt tcactgtaat ttgtggcagt tgctttgctt ttgctttaaa aaagtcttgg
     5161 tataattgtg atgcccgttc atcatcaacg cattcaacct caataccgtg agcccgtaac
     5221 gtctcatcac catgtgtgtc taacgaattg tcttttgttg cgtatactac ttttgctatc
     5281 ttacaatcaa ttattttgtt aacacagggt ggtgttgaac caaaatgact acatggctct
     5341 aacgtaatat aaatcgtcgc accttcagca ttttgttgtg ccatatcaag tgcttgaacc
     5401 tccgcatgct tgtcaccttt tctcaagtgt gcaccaatac caacaatcct accttcttta
     5461 actacaacag cgccaacggg tggattaaca cctgtttgac cttgtaccat atttgcaagt
     5521 tgaatcgcat aatccataaa ttgactcaaa tgatcacctc tataaacaaa aatcctcaca
     5581 tcatgaatta agatgcaagg agaaaaattt atcgttaaat aagcctattt gtacacattt
     5641 ttacaaatac gctacattat ctttgtcgat aattaacatt ctttctccca tccagacttt
     5701 aactgtcggc tctagaatct cactagatca gccactaata tgaaacatat tagcaggtcg
     5761 caggctttat ttactgccgg ttgggaattt caccctgccc cgaaagaatt atatatgaaa
     5821 ttgttataga ttatttgagt acgtagtatg tcaactacat ttaaaatgat actatatgtt
     5881 ttctgaaaaa acaattaatg acggttttaa tttaatataa tctgagtact ataggcatct
     5941 cattgatatg attcttacta acagacatta aaatcaaacc ttcaattcgt ctctatagag
     6001 cgttctcttt attatcttct agttacaaat tattgattgt cactgcgctg ttgttgctca
     6061 ttcgattcta aagcatcata taattgagat actgtatgcg caacttgttc tacaatcatt
     6121 ttcacaccgt ttcgtagttt attaacaccg tttgtcattt gacctatcgc aatcatattt
     6181 gttaatgttc caaaccttgg actaataact tgattggttt ccggaatgat ttgtatgcct
     6241 cccattgggt gtgcttgtac aatttgtcta ttttcaagat ttctaattaa ttgatcatct
     6301 tgatccaatt catttaaatg actttttgca cctgtcgcgt taatgacaac attatatatg
     6361 tctactgatt cttggttttt gtatgaaaaa taatacaact tgccatcatg tttcacatct
     6421 tctaaatctt ttttcaaaat taaagactta ttttctatta attcaataat tagttcagca
     6481 gttcttggag gcattggatt tgaatttaat tgaatcatct ttgagtattt ttgattaaat
     6541 tgatgttggt cttcaatact taagctattc catatccaat ttaaattctc tttcaaatgt
     6601 tcaatcatac tttggaaaat gcccatttct gttggacgcg ctaaatcata cttcaaatct
     6661 gcaatatgat ttcctgtacg tctatgtact aattttttaa aatcaatgtc ataatcagca
     6721 cattctttta aaaataaaga aactaaagta tcaagcggtg cattgccgaa atgatgtttt
     6781 ttaatgtcat ttaatttgtc tttagttaag tacttgaatg tcacgtctat cattgtacct
     6841 cttacacttg gtaaatgagc agaacgactc gtcatagtaa ttggtaattt tggatgatga
     6901 gcagcaacat aacggacaac atctaaactg gcaaggcctg taccaataat cgcaatatcg
     6961 tccagttcat ttacttcgtc taacgtatta tatgttggat aaggcgtagc gatatatcct
     7021 tttttaccct ttaagttata tggatcatgg taggcaaatg taccacatgt taaaaataca
     7081 taatcgtacg cttgccatga ttgtcctgaa tttgtagtac atatgtaata agttaaattc
     7141 gtttcatcga tattagaatt tgtataaatc tcttgaactt tattataatt agttgatata
     7201 tttggatatt ttttcgtgaa catagataaa taagatttca tataatgtcc gaatacaaat
     7261 ctcggtaaat atgcaggttc atcaaaatta aaatcagttt gttgtttata ccacttccaa
     7321 aattcagtct catcatctaa atttaaactc atctttttcg aaggcatatt aattagcagc
     7381 tcagaactat cattttgaaa tggtacgccc tgtcccatat ttactttatc atcgtataaa
     7441 tctatatcta attgattaaa cttcgggtgc ttaactaact ctctcaatac acttacacca
     7501 gcagttccca tgcctattat tgctacacgc atatatcatc catccttttg taattcaaaa
     7561 tattatattt aaactattac ctataattat agcaatttgg tatttccaat aaaatttatt
     7621 aaggtaagct attatttcac tttcacaacc attaaaccat ttaaaacttt tcatttaata
     7681 tcaaccttag tgtttgtgtt aaaatatttt taacgaggtg aattaaattg aaatttatag
     7741 caaagatatt aatctcatta gtaattctat ttatgatttt tagtcgtaaa aacaaagctt
     7801 cttaatcaat acccttgatt acaaaataaa ttcaactaag gtgattggcc atacactcaa
     7861 gatatggcta atcacctttt tcaatgcaga acaaagagta tattttgcaa aatcaatatc
     7921 tataatttat ctttaatacc cttttaatac taaaaccaaa tttaaattac atagtcatat
     7981 cgtgtaatat accaaaggaa catagacatt catctatata gatgataatc tatttaacgt
     8041 ctaggaggta tgttatgacg tataaagaac tagcaacatt tttaaaagtt ttatcagatt
     8101 caagcagatt agaaatacta gatttacttt cttgtggaga gttatgcgct tgtgatttgt
     8161 tagcacattt tcaattctct caacctacac ttagctatca tatgaaagca ttagtaaaaa
     8221 ccaacttagt tacgacacga aaaatcggaa ataaacattt ataccagctc aatcataata
     8281 tttttgagtc cgtaattaat aacttgtcta aggttcatac ctctaaccaa cgatgtattt
     8341 gtcataacct taagactggt gaatgctaat gatgacaact ttagcgacac tcatttttct
     8401 agtaacttta ttatttgtat tatggcaacc taaaggccta gatattggca ttaccgcact
     8461 aactggtgcc tttattgctg ttattactgg tgttgtaagt ttttccgatg ttttcgaagt
     8521 aacaggtatt gtttggaatg ctactttgac ttttgtctca gtcattctta tttcattaat
     8581 attagataaa gttggattat tcgaatggtc agctattcac atgcttcatg cttcaaaagg
     8641 caatggttta aaaatgttcg tttatatcat attattgggt gccattgttg ctgcattttt
     8701 cgcaaatgat ggcgcagcgt taatcttaac gcctattgta ttagcgatgg ttaaaaatat
     8761 aggttttagt aagcgggcca tattcccttt tattattgcg agtggtttta tagctgacac
     8821 aacttcttta cctttaatcg tgagcaatct agtgaatatt atatctgctg attattttca
     8881 tgtaggattc gttcgatatt ttagtagaat gattatacct aatttattct cgcttttagc
     8941 aagtattata gtattgtggt tatattttag aaaagcgata cctaaaacgt ttgatgataa
     9001 taatataaag catcctaaag atgccattaa tgatttaaag ctatttaaaa tttcatggat
     9061 tgttctagtt atattacttt tcggctatct aatcagtgaa tttactaaaa ttccggtatc
     9121 aattttcact ggaatcattg cttttatttt tctaatgttg gctcgtaaat caaatgctgt
     9181 aaatattaag caagtcatta agggcgcacc ttggaatata gtattatttt caattggtat
     9241 gtatatcgtc gtattcggct taagaaatgc tggcattact ttaatattgg ctaaaatatt
     9301 agaatatatt tccaattacg gtctatttag cactattttg ggaatgggct tcatttcagc
     9361 gtttttatca tcaataatga ataatatgcc tacagtttta atagatgcga ttgctattgg
     9421 tcaatcaaat gtccatggca tgttaaaaga aggcctaatt tatgcgaatg ttatcggttc
     9481 tgatttaggt ccaaaaatta caccgatagg ctctttagct acattactgt ggttacacgt
     9541 cttaacacaa aaagatgtta agatttcttg gggcacatac tttaaaactg gtatcatcat
     9601 tacaattcca gtactattta taaccctcat agggttgtat ctaacactta tcatatttta
     9661 attaattagg agattgttat gactaaaaaa acaatttatt ttatatgtac aggcaactca
     9721 tgtcgaagtc aaatggctga aggttgggct aaacaaatct tagcggatga ttggaatgta
     9781 tattctgctg gtatcgaaac acacggtgtt aatcccaaag cgatagaagc tatgaaagaa
     9841 gtaggcattg atatatcaaa tcatacatca gatttaatcg ataataatat tattaaaaat
     9901 tcaaatttag ttgttacatt atgtagtgat gcagacgtaa attgcccttc tttaccaaca
     9961 aatgttaaga aagaacattg gggatttgat gatcctgcag gcaagccttg gtcagagttc
    10021 caacgtgtaa gagatgaaat taaaatcgca attgaaaatt tcaaatcacg atgagagtgg
    10081 gacagaaatg ataaaaaaac actaatgatt tattatgtag tggttcttta tcattagcta
    10141 ta
//
LOCUS       DALAGF010000093        10131 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.093, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000093 DALAGF010000000
VERSION     DALAGF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10131
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.093"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(28..1086)
                     /gene="hisC"
                     /locus_tag="O2692_002162"
     CDS             complement(28..1086)
                     /gene="hisC"
                     /locus_tag="O2692_002162"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCZ4299778.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            complement(1326..2840)
                     /locus_tag="O2692_002163"
     CDS             complement(1326..2840)
                     /locus_tag="O2692_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCZ4299779.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(2833..3810)
                     /locus_tag="O2692_002164"
     CDS             complement(2833..3810)
                     /locus_tag="O2692_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4299780.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQLLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(4032..5813)
                     /gene="recQ"
                     /locus_tag="O2692_002165"
     CDS             complement(4032..5813)
                     /gene="recQ"
                     /locus_tag="O2692_002165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCZ4299781.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(5825..7708)
                     /locus_tag="O2692_002166"
     CDS             complement(5825..7708)
                     /locus_tag="O2692_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCZ4299782.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LIEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREENEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(7979..9919)
                     /gene="ltaS"
                     /locus_tag="O2692_002167"
     CDS             complement(7979..9919)
                     /gene="ltaS"
                     /locus_tag="O2692_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HCZ4299783.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
BASE COUNT         3106 a         1842 c         1381 g         3802 t
ORIGIN      
        1 tatcatttct gtcccactcc ccacttacta ttcgtattta aagtttgata aaacttctaa
       61 cattttatca ttttgttctt taaaaccaat tgtaattcta acaccagttg gaaatggtcg
      121 cgtaatacac cctacattta aaagtgcttc ataaagttca tttacccgct ttgtttttac
      181 aaatataaaa ttcgtttgac ttggcaagaa atactcactt tgaggtaatt gataaaatct
      241 ttcgcgttca acactatttt tatgtgttac ctcttttaaa tattgttgat cttcaagtgc
      301 tgcaactgct gcgtattcag atatacgtgt cacattaaat ggtggtctaa tgatgttcca
      361 tttttcaatc gcatgttcac ttgctaccac atatcctaca cgtaaacccg ctaatccata
      421 cgcctttgaa aatgtacgta ataagaaagc attgtcatat ttttgttgca aagcaagtgt
      481 atccgggtag tcctctgctg tcacaaattc aaaataagct tcatctataa ttactggtac
      541 atgtggaggt acttgagata aaaacgaatc taagctctca tgattaaaat atgtacctgt
      601 aggattattt ggattacata accataccaa tgacgtatct tcattaactt cttttaaaat
      661 accttctaaa tcgaagccac catcttttaa aggtacttgt atcacattag ctgattcaac
      721 aatcgcattg tgataatatt gaccgaatgt cgcttcactt gtaacaatag tatcccctgg
      781 cgttaataca gctctagaaa tcattaatat aacttcatct aatcccgcac caaataaaat
      841 gcgtgattga tctacattta aatgtttact aatcgccgct tttaatgtcg gtgatcctgt
      901 ttcaggataa taatataact catctaagtg tgctgatatc gcttctttaa ctttaggcga
      961 tggtccatac aaattttcat ttgatgcaag tttatataaa tctccttcaa tgccatactt
     1021 ttctttcaat gcccttggag ataaaccagg ctgatatgct gatagttgat taagttgttc
     1081 tttcataata aaaacaacct ccttagaatt aactgaaatt tcaaaaaact tatattcatt
     1141 atatcgctct atgtttctaa aatgcaatgc tactattttc acatcataat agcttacatt
     1201 gtgtttaaca tgtcatattt tttaatcatt attgcatgtt taaattgaac ataattatag
     1261 atttaaatat aacaacgcca cataagatat aatttggtgt tacgcatctt atgtggcgtt
     1321 ggtcgttatt tgattaaccc ttttgctttt aaataatctt tcgcaactgt atatgggtct
     1381 tcatttttaa ctgttacttt atagttcatc atttgcatat cttcatcaga tattttgttt
     1441 tctagtttgt ttaacggttt cttaatttct ggatgtttct ttaaaaagct ttctttaaat
     1501 aatggtgctc cttgatatgg tggaaatacg tgcttatcat cttttaacac aaccatatca
     1561 tattgtttta attcagcgtc agttgaatat gcatctatta aattaatatt acctttattg
     1621 atcgcttgat atctcaactt aggttccatt gtacgtatgt tatctaaatt taaattataa
     1681 gccttttgaa cagctttgta accatctgga cgatcattaa attccaatgt aaatcctggt
     1741 ttaagttgat ctttaacctt atttaaatca ccaattgtac gtatattatg ttgtttagca
     1801 aaatcacgtt ttacagctaa agcatacgta ttgttatact tcattggttt taacatagtc
     1861 atttgatatt tcttttcaag actttgctta gcttgttcat aaactttttt ctcttctttt
     1921 gacttcaatg gttcttttgt taattcacct aaaactgttc cagtaaattc taaataccca
     1981 tctatatcgt cagattttaa agcattaaat aaaaatgctg ttttgcccat accatctttc
     2041 acttctacag tatttttggt ctcttcttct attaaaattt tatacatatt tgtaataatc
     2101 gatggctcgg agccaagctt tccagctaac gtaattttat cacctttttg tgcaaacata
     2161 ggaatagcga tagccagtat aataatcatc actataacac ctaacgtcat caataacttt
     2221 ttataagata atttagccat aaatcttaaa attaaatcaa atataattgc caataaggct
     2281 gctggaattg cacctaataa tatcaacgat gcattgttac ggtctatacc taataaaatt
     2341 aaatctccta gtccgcctgc accaattaat gctgctagtg ttgctgtacc tataattaat
     2401 accatagccg ttcttacacc agccattata acaggcattg ctatcggaag ttcgacttta
     2461 gttaaacgtc taaatggttt catacctata cctttagccg cttcaatgag tgatggatca
     2521 acttctttaa ttccagtata cgtattcctt aaaattggta acaacgcata cactacaagt
     2581 gcaataattg ctggcacacg accgatacca aataaaggaa tcattaaacc taataatgcc
     2641 aacgatggta tggtttgaag aattgccgca atattcatta cgatttcaga tatcgtttta
     2701 gtcttcgtta ataaaatacc taatggtacc gcaatagcag ttgcaatcaa taatgcgata
     2761 aatgatattt gaatatgttc tatcattgtc gaaaagagtt gccccttacg ttcactcaat
     2821 atgtcgaaaa agttagtcat gttgagctac ctcctttttc tgggacaaat atttgaagat
     2881 atctttccta tcaataacat attgacctac gctatcttct tgcatgacaa tgacacgctc
     2941 gctctctgat aaaagttgat acaatacttc aattggttga ttgtcataaa caattggata
     3001 agcgctcata gatgtaacct catcgattgg tttcataata tccaagtcac ggataattgc
     3061 gttctcttca acacatggcg catcatcttc taaatgacta cccataaatt gtttaacaaa
     3121 ttcactttga ggattatttt taaatccttc tggtgtgtca atttgttcaa tatgcccttc
     3181 attcaaaaga caaatcttat caccaagttt catcgcctct tgaatatcat gtgtaacaaa
     3241 tatgattgtc ttcttaattt tagtttgtaa ttcaattaaa tcatcttgaa gtttttctcg
     3301 gctgattggg tctaatgcac taaacggttc atccattaaa ataactggtg gatcagctgc
     3361 taacgcacgt ataactccta cacgttgtcg ttgcccccct gacaattcat caggttttct
     3421 gtttttatat ttttcaggtt ctaatccaac catttcaagt aattcatcta ctcttttatc
     3481 tatatctttt tctttccact ttttcatttg tggcacttgt gcaatatttt ctttgattgt
     3541 catatgtggg aataatgcaa tctgctgcaa tacgtatcca atatcccaac gcatttcgta
     3601 tactggataa tcacttattg gtttatcttt aaaataaata taaccttcac ttaagtgaat
     3661 gagtcgatta atcattttta atgtcgtagt ttttccacaa cctgaaggtc caattagcac
     3721 aaaaaattca ccctcattaa tattgaaact aatgttatcg acagcaacat gtttgccata
     3781 acgcttagtt acatttttaa acttaatcac tttgccacct ctttttttct catagcataa
     3841 aaccgagatt atatgtatgt attccctatt taaccacgtt tattacaatt ttcaaattta
     3901 aatgatttat ccttgaactt ttttaataaa ataatgaata ataggtaatc tccagttaag
     3961 aaatagtgtt attttacctt gaattcaaaa aaacacccag taaaacaagg aatgcttact
     4021 aggtgtcttc actatacttt ggctttataa ttttgaatcg tttctaaaaa tgctggacaa
     4081 taatgtttta atttgtaact acctacgcca tcaatattaa tcatatcttg tttcgaagca
     4141 ggcttacgtt tagcaaattc ctccaacgtg taatcagaaa atatacttac aggtgctatc
     4201 gttaatttgt cacttaactt tttacgaact tctaccaact gactgaataa tactcggtca
     4261 accccttcaa ccgtatttat aaatactttt tcagtcgctt tttgcttaaa tggtgttgtg
     4321 aatacttcta cttcattact gagtaatttt ttaattgaag tatcacacat taatatttcg
     4381 tcattttcat ttaagaaccc tttgaatctt aattcatcta ttaagtgact taattctgat
     4441 gttgtgtaac ctttcattaa accatgggtt gaaatttggt cataaccttt atacttaata
     4501 taatctgttg actctcctct taacacttga atgataacac tataactctc ttgttgtttc
     4561 atacgagcga tgcaactaat aatcatctta gcttcttgtg tcatattata tgatttatct
     4621 tgttgaacac aattactaca ttgttcacat tcttctaatt tttcattcgg ttcaaaataa
     4681 tggacaattg ttgcttctag acattttttt gtttttgtat attgaatcat tttagttaac
     4741 ttttcgccca ttttatcttt atagtcatca tcagcttgag agactgttat aaaatactcg
     4801 tgtaaattga tatcgcgttc gctaaataac aaaatacatt cacttttcaa cccgtcacga
     4861 cctgcacgac ccgcttcttg ataataagat tctaaatctc caggcatatt ataatgaata
     4921 acaaagcgta cattggattt atcaataccc ataccaaaag catttgtagc aacgactact
     4981 ttaacacgat caaataagaa atcattctgc gcttcttctc tttctttatt gctcaaacct
     5041 gcatgatata taacactttc aattttctga ctttctaagg cttcttgaag ctcttcaact
     5101 tgcttacgtg tagaacaata aataatacct gcatcttcat cgtgtgtttt aatataatcc
     5161 aatataaatt tttgacgttg ataagtagga tttactttaa aaattaagtt tctacgctta
     5221 gtactcgttt taatttgatc agtttgagcg atatttaact tttctctaat atcttgctgt
     5281 acttcaaccg tggcagttgc tgtcaacgct attattgtaa aatcttgagg taacgtaaat
     5341 acttttgaaa taacattttg gtaactcggc ctgaaatcat gaccccattt agaaatacaa
     5401 tgcgcttcat caaacgcgac taagtgaatc tttatacgct gaagcatatt taaaaaatat
     5461 cggttttcaa atcgttctgg tgcaacatac aaaaattgaa tttctccatt tgataatgct
     5521 ttttcaatac gttgttgctc tttttgagtc aaactactat ttaaaaaagc agcttgaatt
     5581 cccatcgctt ttaattgatc cacttgatct ttcattaatg atattagtgg acttattaca
     5641 attgttgtac cacctaacaa taaacctggt acttgatagc atatagactt acctccacca
     5701 gttggtaaga caccaagcac attacgatgg tctaatactt tgctaataat ttcttcttgt
     5761 cctggtcgaa acgtttcata cccaaagtaa tgcgataatg tttgttgcat cataaattga
     5821 ccccttattg ttgttcttta atttcttcta actcactcca tcttgtgatg tctaaatcat
     5881 attgaatttc aagttgttct ttttcttcgt ttaattcttt aattttccca taatctgcac
     5941 ttgcctcaat catgagcaca tcaatttctt ccattcttac ttccgcttgt tctatgcgtt
     6001 tcatcaattg ttcatattct aatttttctt tatatgataa accatttttc ttacgtacag
     6061 ttgtagaaga tttagattgt tgcttcaatg tggatttatt tttatctaat gattttttat
     6121 aactttcata atcttcaaaa gttccgataa tcttttccat ctgaccatca tgaataaacc
     6181 aatatgactg tgcaacttta tttaagaagt agcgatcatg gcttacggta atcactgtac
     6241 caccgaaagt atgaatataa tcttcaagta ttgttaaagt ctcagtatct aaatcatttg
     6301 tcggttcatc taacaacaga acatttggct ggtgtacgag tagacgtaat aaatacaaac
     6361 gcttttgctc tccaccagat aatttataaa cttttttacc atgagttgca cttggaaata
     6421 aaaatcgttc aagaagttgt gtaatagata ccgaggttcc atctttttct tttgcgatct
     6481 cattttcttc tcttaaataa tcaatcatac gaatatctct atccagggtc tcatctgttt
     6541 gcttaaaata agctacttta accgtctgcc cagtcttcaa tttaccttcg aattgttggt
     6601 cttctccact caaaatattt aagagtgttg ttttaccagc tccatttggc ccaacaacac
     6661 caatacgctc acctttttga ataatttccg tcagatgttc aattaatact ttatcattaa
     6721 tagcctttga taagtcttct aattcgaaca cttgcttacc taatcttgaa taagcaagat
     6781 tcaattcacc tttatcgtct ttatattgct ggttaacttc attttctagg tcattaaatc
     6841 gattaattct agcttgttgc tttgtagtac gagccttagc tccagccctc atccaagcaa
     6901 gttcttcctt atataaagct cgttgctttt gttgttgctt ttgaagtgtt acttctcttt
     6961 cagcgcgcat ttcaatataa gattcatagt taccaggata tgacgctaac ttacctctgt
     7021 ttagttcaat aattctagtg gaaacttcat ttaaaaaata tcgatcatgg gttacgaata
     7081 aaacagtatg aggatattgc ttcacataat tgatcaacca gctgattgat tcgaagtcta
     7141 aatggttcgt aggttcatct aataacaata aatctggttg ttctattaat gttttagcaa
     7201 gtacaacacg tttttgttgt ccgccggata attctttaat gtatttagta gtatcatgta
     7261 tacctagttt tgagaggatt gttttaattt cagcgttata gtcccaagca tcatgttgat
     7321 ccatagcatc ttgcgcatcc atcattcgct tgaacaattt atcactttgg tcatccgcat
     7381 aagcttgtac tgcctgctca tattgcttga tgatgcgtaa agttgttgta tcagaactta
     7441 atactgcatc aaaaactgtc atatcttcat taaggtcctg tttctgagac gaatatcgaa
     7501 ttcgatattg atttggatgc ataacattgg ctgtaaaatc atcatcaata ccaccaatta
     7561 cttttaacaa cgtacttttc cctgtaccat ttatgcctac taaacctatt ttttcacctt
     7621 ctgaaattga taaatctagg ttatcgaata tagtcttatc ggcataagat ttatttaaat
     7681 gttcaatttt atatgcttcc atacccatct tccttcttct gttgaaatct ctacgtactt
     7741 aaatcagttc tatacattat acaccttttt gaaatcaaaa gaaatcgata ccgttaagtg
     7801 tcataattta atatattgtt gcttcaacta taaataaaac ttgttaaggc tttgatgact
     7861 atgctaatga tctttataat ataccttttt atcaacacat ataatgtaaa acataatata
     7921 aaagaacata ataaaaaccc ggattgatat tttatcaatc cgagttcgtg tttagatatt
     7981 attttttaga gtttgcttta ggtcctgttt catatttata cttcgaagga tttacctttt
     8041 tgaagtctgg atttttgtag aatctaaaca aatcaccatt aagcacgttg tcactcattt
     8101 cgagatcctt ttcaacttgc tttttattct tttcgaaatc agctggttga gtagttatga
     8161 gttcattatt tttattagaa taaatcttac cattaacata tttataatct tttgttataa
     8221 agtcaccatt tctgaatgga actacttgat tatgaccttt agagaataaa tcagtaccga
     8281 acattaaata gttcttcgta tctataccag ccaaatgtaa aattgttggc attacatcga
     8341 cttgaccagc atattcatta ttgataccac cagatttacc agggatttta atccagaaac
     8401 cagttctgtt taaatctgta aatttagccg gtgtgatttt ttcacctaat agtttttcca
     8461 tggcattgtt atggttttca gagataccat agtggtcacc ataaatcata atcactgaat
     8521 tgtcatataa tccttttttc ttcaagtcat taatatattc ttctaatgct tcgtctaaat
     8581 aacgtgctgt ttgaatataa ccatcaactg ttgcatcacc tgtgtttgat ttctcaatag
     8641 ttgcatcctt ttcatctaat gtgaatggat agtggttagt caatgtaatt aaatgagaat
     8701 agaatggtga tttcatctta gcttgataat tagcagaatc tttaaagaaa attttgtctt
     8761 tcaagcctaa gtttacaacg tttttatctg acatgtcata gtatgttgca tcatagaatt
     8821 tatcgatacc aaagtgttta tatacttggt ctctgttcca gaatgtttta tagtcaccgt
     8881 gcatgacatc agatttgtag ccttgctttt gatctaaaat tgctggtaat gactgatacg
     8941 tattatctcc ttttaatgaa aaggcagaac cttgcggtaa accgtataaa ctgttatcca
     9001 ttgtaaattc agagtcagat gttttacctt gacctgtttg atggaaaaag ttagggaagt
     9061 atgtgaattg ctctttccca cttgataatt tgtttaaaaa cggtgttact tctttaccat
     9121 taaccttttt attaattaag aaggtttgga aactttctaa atgaatctta ataatatttt
     9181 tcttctttgc caccccataa tattctgggt taggctctgt ttgacgttgt ttcgtataat
     9241 ttaatacttt tgttaagtca tcttcagatg ctagcgcttt ttgttgatta ttttcgatag
     9301 ttttaacacc atcgtatact gtaaagttat aaggtcctaa atatttcact aaatatttat
     9361 ggtcaaatgt acgtgttaat aattctggtc tgtcagtttc agcaaaagct aagtttaaga
     9421 agaataatgc tactgaagcc gccattacga caggaacaaa tttcttacta aatgcttttg
     9481 tgtctaacca ttttgtttta aatattaaaa tgaataagta aactaacgta tcaatgaaat
     9541 aaacaaagtc ataccatttg aatgatgcac taaccgcacc acccatagat tctacgttac
     9601 ctacttggtt taaagtacta aacgttaaaa aatcagagaa gaatctaaag tacacaacat
     9661 tggcatataa taggaacgtc aataagaagc cgcctatgaa catgaaccaa aatgcttttt
     9721 tgcctttaaa gaataggaac acacttaaaa ccagtgctac taaactataa ggattcatca
     9781 ataatattaa gttttgtact aaacctttaa cacctaaaga aaaatcaaca taataagaaa
     9841 aatacgtctt caaggtaatc gttattacgg ttaataagaa gaacgcaaaa agactaattt
     9901 tctttttttg tgaactcatg attctttccc ccgttattta gataataaat cttgcgaaag
     9961 tacttgaata aattagatat taacattatg tagttaatta ataagtgcgt ttaaaagtta
    10021 acattgtcat aattatagta attactttca gattcaagtt tataattttc gttatttgta
    10081 gctttcaact ttgatttaac aagtattttg ggctacaact catatttgct t
//
LOCUS       DALAGF010000094        10109 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.094, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000094 DALAGF010000000
VERSION     DALAGF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10109
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.094"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(217..1992)
                     /locus_tag="O2692_002168"
     CDS             complement(217..1992)
                     /locus_tag="O2692_002168"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCZ4299784.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHISGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKIDVTTSQKIAREILIDRNGNAE
                     SIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFT
                     INRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSPFDLRVLMDAIYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
     gene            complement(2166..3038)
                     /locus_tag="O2692_002169"
     CDS             complement(2166..3038)
                     /locus_tag="O2692_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCZ4299785.1"
                     /translation="MNKLQDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLI
                     LATIILLPFVIIKKLPTPELRDIPVIFILGFCGFVIYHTALNFGEALISAGISGILVS
                     TTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVFIILLAS
                     FSESLYFTFQKKYIEKYGFIAFTLYTIMASSPFMLIFIPEIINDIHGATFTSIVSVLY
                     LAIFPTIIPYVLLAYIVKSVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGII
                     TLLGVSLSNFFQNT"
     gene            3151..4548
                     /locus_tag="O2692_002170"
     CDS             3151..4548
                     /locus_tag="O2692_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCZ4299786.1"
                     /translation="MAKYKDIASDIRDKIITGDWFYGMKIPSHRQLAIQYNVNRVTII
                     KSIELLEAEGFIYTKVGSGTYVNDYLNEAHITNKWSEMMLWSSQQRSQYTVQLINKIE
                     TDDSYIHISKGELGISLMPHIQLKKAMSNTASHIEDLSFGYNNGYGYIKLRDIIVERM
                     SKQGINVGRENVMITSGALHAIQLLSIGFLGQDAIIISNTPSYIHSTNVFEQLNFRHI
                     DVPYNQINEIDTIIDRFINFKNKAIYIEPRFNNPTGRSLTNEQKKNIITYSERHNIPI
                     IEDDIFRDIFFSDPTPSIKTYDKLGKVIHISSFSKTIAPAIRIGWIVASEKIIEQLAD
                     VRMQIDYGSSILSQMVVYEMLKNKSYDKHLVKLRYVLKDKRDFMLNILNNLFKDIAHW
                     EVPSGGYFVWLVFKIDIDIKYLFYELLSKEKILINPGYIYGSKEKSIRLSFAFESNEN
                     IKHALYKIYTYVKKV"
     gene            4738..5211
                     /locus_tag="O2692_002171"
     CDS             4738..5211
                     /locus_tag="O2692_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HCZ4299787.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYPKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
     gene            5473..7065
                     /locus_tag="O2692_002172"
     CDS             5473..7065
                     /locus_tag="O2692_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCZ4299788.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISVDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
     gene            complement(7394..8920)
                     /gene="spa"
                     /locus_tag="O2692_002173"
     CDS             complement(7394..8920)
                     /gene="spa"
                     /locus_tag="O2692_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HCZ4299789.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQLNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDNNKPGKEDNNKPGKEDNNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNKKPGK
                     EDGNKPGKEDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIK
                     PGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL
                     "
     gene            complement(9341..10093)
                     /gene="sarS"
                     /locus_tag="O2692_002174"
     CDS             complement(9341..10093)
                     /gene="sarS"
                     /locus_tag="O2692_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HCZ4299790.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE"
BASE COUNT         3244 a         1490 c         1758 g         3617 t
ORIGIN      
        1 gacttgtaaa atatcgtcac ttattatgta aattttcagt gtgaaatggc aggtttgcaa
       61 tcacttgttt aacaaaatga tgcaatcaat catgtaatta tgtttcatca aaaaaatcat
      121 gagagtggaa caacgaaata atttttatga aaacatcatt tctgtcccag tctcatgatt
      181 tgaaatcacc aaataaaaat ctattaatgg ttttcgttat aacaatttgt gttcttttaa
      241 taatgactca atgtacgtac cttttatctt tttaaggaat cctgctaatg cgagtttttg
      301 cattttcgaa tctttagtaa tctcacgcaa atcttggtgg tcattcagtt cgtatatggc
      361 atccattaag acgcgaagat caaatggact attgatgact tctggaatac cacgatctat
      421 atttagtaat tgataaacag cttccatggc agtacgaacc gaatattctg ttgtaaatac
      481 agtgtctcgc tctgtttctg caaagttacc aataaatgct aagttctgag attgatgcgg
      541 gacgactaaa ggtctgtcgc cgatagcacg cgtcatgaaa taagatgtga tatatggcat
      601 ataaacagga atcgtattag atgcatgttt tgctaagtct tcaattttgt cagttgatac
      661 acctaagtga tacagccatt cttggcatat ttcattacca ctacattctg taattggctt
      721 tttaatataa tcgccgttta catctgaata taaggcataa atccatgtag atatttcatt
      781 ttcaggttgg tctttaaact gttgctgacg attgattgta aaactcattt gccatgcaga
      841 atcattgatt gtaataatac cgcctgtaac tgttttgcct gcaagtgggt cacgtttaca
      901 aatactttct attgtatcga taatctcttt attgtttgtt gtagaagttg ctgaaacaaa
      961 ccaacttttt ttaggaatat tttggcaaaa cttatcagga ttaccaaatt caggactttg
     1021 tcgcgctaaa tttttccata gtgtccaact accacctaat tcgtcagttg gtggcgctgg
     1081 tgtatcatta tcaccataag tagagctttc tgtaatacta ccgtttgtca caaagacaag
     1141 atcgtttata gtcagtttaa tagattctgc attaccatta cggtcaatta atatttctcg
     1201 ggcaattttt tgacttgtcg taacatctat tttaatatct tcgactttta catcgtattc
     1261 aaattgaacc ccatgcgatt ttaaatattc aaccataggt aatactaaag attcatattg
     1321 attatattta gtgaatttta aagctgaaaa gtctgcgaga ccactaatat gatgaacgaa
     1381 tcgcattaga tagcgacgca tttccattgc agaatgccac ggttcaaatg caaacatcgt
     1441 tttccagtaa atccaaaagt ttgaattaaa gaagtcatcg gaaaatacat ctgttatttt
     1501 gacatcatct aaatcttctt cattcgttaa gcataaatct aaaatttctt taatcgccgt
     1561 tttagtcaaa gtgaagtctc cgtctgtgac taaacgttga ccctgtttct caataacacg
     1621 acagcgagaa tagttagggt cttctttgtt tagccaatag aactcatcta atacagacgc
     1681 gttatcgatt tctaatgaag ggatagatct gaataagtcc cacaaacatt caaagtggtt
     1741 ctccatttca cgaccaccgc ggacaacata gccttttaaa ggcatatttt caccatcaag
     1801 actaccacct gctttaggta actcttctaa aatatgaatc ttcgaacctt ccatttgacc
     1861 atcccttatt aaaaaacaag ctgcagcaag tgaagctaga ccagatccga ttaagtaagc
     1921 ggatttgttt tctacatttt caggtttttt agggcgcgca aatgcttcat aatttccata
     1981 actgtaatac atatccacca agtcctttcg tattagaata cactcagact ataccccttt
     2041 gtgaattgca aatattagta tttatattgg taaatttgac gaaattagat catttttata
     2101 tttttgattt ttaaaatttt atcactcgaa tgtttttgga ttctgaggtg cgggacttgg
     2161 aataattatg tattttgaaa gaagttactt aaactaacgc ctagtagagt gataattccg
     2221 cctataatag caagggttgt tggtagctcg tctaataaca gataagataa taataaagaa
     2281 acgataggtg ttaaataaag agacattgtt gcatcagaga caccaactga cttcacaata
     2341 taagcaagca aaacgtatgg aattatagta gggaatatag ctaaataaag taccgatact
     2401 attgatgtaa aagtggcgcc gtgtatatcg ttgatgattt caggaataaa aataagcata
     2461 aatggtgagc ttgccattat tgtatatagt gtgaaagcga tgaagccgta tttttctatg
     2521 tattttttct ggaaagtaaa atacaaactt tcactaaaag atgcaagtaa aataataaaa
     2581 acacctaata cattaatagt tgtgtaatca tctttactta ttgaaataat ggatattcct
     2641 ataaatgcga cgagtgaact taaccaattc cattttgaaa aatgctcttt taaaaatata
     2701 taagctaaag cactagaaaa aataggcgtt gtagagacta gaattccaga tatacctgca
     2761 ctaatcaaag cttcaccaaa atttaaagct gtgtgatata tcacaaatcc acaaaatcct
     2821 aaaataaaaa taacagggat atctcttagt tcaggggtag gcaatttctt tataattacg
     2881 aacggcaaga gaattattgt tgctaaaatt aaacgaaatg ccgacaatga ttctgcacta
     2941 aaatcattta acgcaatctt tatcattgga aatgcagatc cccacaatat gatagtaaat
     3001 aaatatgata gaaaagtagt gtcttgaagt ttattcatta atatcatcac tcctttaatt
     3061 atgtgtttct atattaaaaa atatgattta aaatgagtac aaccaatttt gaatggattt
     3121 acctatccaa ttttaaaagg gagggagaag atggctaaat ataaagatat tgctagtgac
     3181 ataagagata aaataatcac aggggattgg ttttatggaa tgaaaatacc ttcacatagg
     3241 cagttggcga tacagtacaa cgtaaataga gtaacgatta ttaaaagtat tgagttatta
     3301 gaagctgaag gatttatcta tactaaagta ggtagtggaa catatgttaa tgactatttg
     3361 aatgaagcac atattacaaa taagtggtct gaaatgatgt tatggtcctc tcaacaaaga
     3421 agtcagtata cggtgcaatt aattaataaa attgagacag atgattcgta tatacatata
     3481 agtaaaggtg aattgggtat atcgttaatg ccacatattc aattgaaaaa agccatgtct
     3541 aatacagcca gtcatattga agacttatct tttggttata ataatggcta tggttatatc
     3601 aagttaagag atattatcgt tgaacgaatg tcaaagcaag gtataaatgt aggtagagaa
     3661 aatgtaatga tcacttcagg cgctttacat gccattcaac ttttatctat tgggttttta
     3721 ggtcaagatg ccataataat ttcgaataca ccatcatata ttcactctac aaatgttttt
     3781 gagcaattga attttagaca tattgatgtt ccttataatc aaattaatga aattgatacc
     3841 atcattgata gatttattaa ttttaaaaat aaagcgattt atatagaacc taggtttaat
     3901 aacccgacag gtcgttcttt aacgaatgag caaaagaaaa atataattac ttatagcgaa
     3961 agacataata ttcctatcat tgaagatgat atctttagag atattttctt tagcgatcca
     4021 actccttcta tcaaaactta tgataaattg ggaaaagtta tacatataag cagtttttca
     4081 aaaacgattg caccagcaat aagaataggt tggattgttg cttctgaaaa aataatagag
     4141 caattggcag atgtaagaat gcaaattgac tatggatcca gtattttgtc acaaatggtt
     4201 gtatatgaga tgttgaaaaa taagtcttat gataaacact tagtaaagtt aaggtatgtt
     4261 ttaaaagata aacgagactt tatgttaaac atcctcaata atttatttaa ggatatagca
     4321 cattgggagg ttccaagtgg aggttatttt gtatggttag tctttaaaat agatatagat
     4381 attaaatatt tattttacga attgttaagt aaagaaaaaa tattaatcaa tccgggttac
     4441 atttatggca gtaaagaaaa gagtataagg ctatcttttg cctttgaatc aaatgaaaat
     4501 attaagcatg cgctctataa aatttataca tatgtgaaaa aggtttaatt aaaacaataa
     4561 ttcgaatcat tatgtggcat gttaaacagc taaatataag ctatgcacat ttaacaagcg
     4621 gatactcctg acgcaattat agtatcatta catattgttt aatcgtttgg ggtaatgtaa
     4681 ttaaaaggta aaataggcag agtgttatac ataaaatgta aagaaagagg cgatagaatg
     4741 tctaaaatca ggtcttttac aatattaagt ctacttattt acttagctat gatgtgctat
     4801 acagtagtga cctatccaaa attaccaacc aaagtgccta ttcattataa tttagcaggg
     4861 gatgctgata attttgctga taaatgggtg ctgcttttga ttaatagcgc atttatagtg
     4921 atttggctta tatttttcat tgcaggtaga tactatgaac gatttgccaa atggtcacat
     4981 tataatcata caccacgtga aattcgagcg attaaattat ttttaagtac gttaaattta
     5041 gagattatga gctatatgtc tatcttcaca gtattagaaa tttggcaaat acaacaccat
     5101 catcaattca atttactatg gtttaatatg atatttatca ttatcattgg tttgacgctt
     5161 gtcatatttt gtttacttcc tacaattcat aaaatgagag attctcaata aaaatataaa
     5221 ctctcatgat gcagttttga agcgaattcc tgagattatt ataaataata tctagtattt
     5281 ataattaaaa tgatatatta tttattatag ttaaatattg tgtatatttt gtgaactttt
     5341 tgtaaaaatt cgattgcctg tcacatatag gagtgttaca ttttaaatat gtgatcatcg
     5401 caaaatataa gttgaaatag gttgtagatt aatcagaatg ataaatattt tatataaaga
     5461 gagggagtca ttatgacact acttactgta aatccattcg ataatgtcgg attatcagcc
     5521 ttagttgcag cagtacctat tattttattt ttattatgct taaccgtttt taaaatgaaa
     5581 ggcatttatg cagcattgac aactttggtt gttacattga ttgtggcttt atttgtattt
     5641 gaattaccag cgcgtgtatc agcaggtgcg attacagaag gcgttgttgc cggtattttc
     5701 ccaataggat atatcgtttt aatggcagtt tggttatata aagtttctat taaaacagga
     5761 caattttcta ttattcaaga tagtattgca agtatttcag tggaccaaag aatccaacta
     5821 ttattaattg gattttgttt caacgcattt ttagaaggtg cagcaggatt tggtgtgcca
     5881 attgcgattt gtgcagtatt attaattcaa cttggatttg aaccattaaa agcagcgatg
     5941 ttatgtttaa ttgctaatgg tgcggcgggt gcctttggtg caattggttt accagttagt
     6001 attattgata cgtttaactt aagtggaggc gttacaacat tagatgttgc gagatactca
     6061 gcattaacac ttccaatttt aaactttatt attccatttg ttttagtatt cattgtagat
     6121 ggtatgaaag gtattaaaga aattttacct gtcattttaa cagtgagtgg tacatatact
     6181 ggattacaat tattattaac aatattccat ggtccagaac tagcagacat tattccatca
     6241 ctagcaacaa tggtggtgtt agcatttgtt tgtcgtaaat ttaaaccgaa aaacattttc
     6301 agattggaag cgtctgaaca taaaattcaa aaacgaacgc ctaaagaaat tgtctttgct
     6361 tggagtccgt tcgtaatttt aactgccttt gtattagtat ggagtgcacc attcttcaaa
     6421 aaattattcc aacctggagg tgcacttgaa agtttagtaa taaaattgcc aattccaaat
     6481 actgtgagtg atttatcgcc taaaggaatt gcgttgcgtc tcgatttaat tggtgcaact
     6541 gggacagcga ttttattaac agtaattatt acaattttaa ttacgaagtt aaaatggaaa
     6601 agtgcaggtg ctttattggt cgaagcaatt aaagaattat ggttaccgat ccttacaatt
     6661 tcagctatcc tagctattgc taaagttatg acatacggtg gtttgactgt agcaattgga
     6721 caaggtattg ctaaagcggg agcaattttc ccattattct ctccagtatt aggttggatt
     6781 ggtgtgttta tgactggttc agttgtaaat aacaatactt tattcgcacc tattcaagcg
     6841 acagtggcac aacaaatttc aacaagcggt tcattacttg tggcagctaa cactgcaggt
     6901 ggtgtagcag cgaaacttat ttcaccacaa tcaattgcca ttgcgactgc agctgttaaa
     6961 aaagttggtg aagaatctgc attattaaaa atgacgttaa aatacagtat tatatttgtt
     7021 gcttttattt gtgtttggac gtttatacta acgttaatat tctaaatata aataatgttg
     7081 tcacttggat tcaaatgaca ttttaaatct aattattcat gaatcgaact agtacgaaat
     7141 gcaatgagca tcttgtctag ttcgattttt taatgcctaa aaatgtcata tatgtaatca
     7201 gagtagaaag tgttgaggcg tttcagaagt tgtttagaaa agtaagtaaa ataaaaaatg
     7261 cactgagcaa caaaagatgt tgctcgtgca tttagatgat tcttatcatt tcaaataaga
     7321 atgtgttaat caacgtatat aagttaaaat tggtttggat aaaatgatat ctatcgttgt
     7381 gtattgtttg tttttatagt tcgcgacgac gtccagctaa taacgctgca cctaaggcta
     7441 atgataatcc accaaataca gttgtaccga tgaatggatt ttcttcacca gtttctggta
     7501 atgcttgagc tttgttagca tctgcatggt ttgctggttg cttcttatca acaacaagtt
     7561 cttgaccagg tttgatcatg tttttatcag ctaatttgtt atctgcagca attttgtcag
     7621 cagtagtgcc gtttgctttt gcaatgtcat ttactgtatc accaggttta acgacatgta
     7681 ctccgttacc atcttcttta ccaggtttgt tgccatcttc tttgccaggc ttgttgccgt
     7741 cttctttacc aggttttttg ttgtcttctt taccaggctt gttgccatct tctttaccag
     7801 gttttttgtt gtcttcttta ccaggcttgt tgccgtcttc tttgccaggc ttgttgttgt
     7861 cttctttacc aggcttgttg ttgtcttctt tgccaggctt gttattgtct tctttgccag
     7921 gcttgttatt gtcttcctct tttggtgctt gagcatcgtt tagcttttta gcttctgcta
     7981 aaatttcttt gctcactgaa ggatcgtctt taaggctttg gatgaagccg ttacgttgtt
     8041 cttcagttaa gttaggtaaa tgtaaaattt catagaaagc attttgttgt tctttgttga
     8101 atttgttgtc agcttttggt gcttgagcat catttagctt tttagcttct gctaaaaggt
     8161 tagcgctttg gcttgggtca tcttttaggc tttggatgaa accattgcgt tgttcttcgt
     8221 ttaagttagg taaatgtaag atttcataga aagcattttg ttgttctttg ttgaatttgt
     8281 tatccgcttt cggtgcttga gattcattta actttttagc ttctgacaat aggttagcac
     8341 tttggcttgg gtcatctttt aagctttgga tgaaaccatt gagttgttct tcgtttaagt
     8401 taggcatatt caagatttca tagaaagcat tttgttgttc tttgttgaaa ttgttatcag
     8461 ctttcggtgc ttgagattcg tttaattttt tagcttcacc taaaacgtta gtgctttggc
     8521 ttgggtcgtc tttaagactt tgaatgaagc cgttacgttg cgcttcgttt aagttaggca
     8581 tgttcaagat ttcatagaag gcgctttgtt gatctttgtt gaagttattt tgttgcgcat
     8641 cagcttttgg agcttgagag tcattaagtt tttgagcttc acctaaaacg ttagcacttt
     8701 ggcttggatc atctttaagg ctttggataa aaccattgcg ttgatcagca tttaagttag
     8761 gcatatttaa gacttgataa aaagcatttt gttgagcttc atcgtgttgc gcagcatttg
     8821 cagcaggtgt tacgccacca gatataagta atgtacctaa agttacagat gcaataccta
     8881 cacctagttt acgaattgaa taaatgtttt tctttttcaa attaataccc cctgtatgta
     8941 tttgtaaagt catcataata taacgaatta tgtattgcaa tactaaaatc tatatttata
     9001 attaaattta aaggtaagtt ttacaactta taaaataaat attttgctga aagatttatt
     9061 caggaagtaa aaggcttaaa accgcaaaat cacgctattt cgattaataa aaacagataa
     9121 aatatagaga tattttttat aatgaaagcg gtttaataca tatgagttaa aaaatatttt
     9181 taagaataaa gtggggcttt gaatgtgctg aggtttaatt tcggaaatgg ttttaagtga
     9241 tatagaaaat agaaagttgt tcattatgtg ctgaggtttt attaaaaaat agaaaacaca
     9301 agtgcacttt agaatacagc acacttgcgt aatagagata ttattcaaaa acaagatgta
     9361 aatgatcttt atctgctaat aattgattca cttgagctaa taattgttca gcatggtctt
     9421 gctgcgcgtc atccatatga attaaaattt ttctttcatc ttcagttgag cgttctttta
     9481 ttagatagcc ttgctttttt aaattattga gagctctaac agtttgaggg tatttatggt
     9541 ggattgtttc aattaaatct ttaagaagaa cgatgttttt attttgagaa gtgataatag
     9601 ctagaattgt gaattctaca aaacttaatg ttagatgttt tttgataata ttcttgaaat
     9661 acattgtata catcatcaag tttagaaatt ctttactatc ttttggtatc atctgtgatt
     9721 cactttgatc tgcaaggtta aattgtttaa tgatttgatc aaacaatgta acacgttctg
     9781 caattttctc tcgttgttct tcagatattg aaatgtaagt attacgctca tcaattttac
     9841 ttcgaacttt actaatatat gaatgtttca caagtacttt tatatgctgt actaaatccg
     9901 attgtttata acataaatct gaaacaatct tcttaaatgg aagtgtgttt tcttgttgat
     9961 gaaataaata agtcagtaat ataaattctt ttatagtcat atcgacttca ggcttgactt
    10021 ttttcttaaa acgaaacata tatgcttcaa tgattataaa atctctaatt ttgtcatggt
    10081 tattatattt cattgtttta tctccttgt
//
LOCUS       DALAGF010000095         9990 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.095, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000095 DALAGF010000000
VERSION     DALAGF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.095"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(250..1128)
                     /locus_tag="O2692_002175"
     CDS             complement(250..1128)
                     /locus_tag="O2692_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HCZ4299791.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            complement(1148..4588)
                     /locus_tag="O2692_002176"
     CDS             complement(1148..4588)
                     /locus_tag="O2692_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCZ4299792.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNAQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDMSYSTIEDFINSDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQALKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTRVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIINPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLELLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            4892..6232
                     /locus_tag="O2692_002177"
     CDS             4892..6232
                     /locus_tag="O2692_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCZ4299793.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWMIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGAL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     EQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKN
                     TAIKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVFIIELILYIKDRRAKQ"
     gene            complement(6438..6770)
                     /locus_tag="O2692_002178"
     CDS             complement(6438..6770)
                     /locus_tag="O2692_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCZ4299794.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
     gene            complement(6854..7999)
                     /locus_tag="O2692_002179"
     CDS             complement(6854..7999)
                     /locus_tag="O2692_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCZ4299795.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            complement(8208..8587)
                     /gene="rnpB"
                     /locus_tag="O2692_002180"
     ncRNA           complement(8208..8587)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="O2692_002180"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(8650..8994)
                     /gene="gpsB"
                     /locus_tag="O2692_002181"
     CDS             complement(8650..8994)
                     /gene="gpsB"
                     /locus_tag="O2692_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCZ4299796.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            complement(9008..9571)
                     /locus_tag="O2692_002182"
     CDS             complement(9008..9571)
                     /locus_tag="O2692_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCZ4299797.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            complement(9564..9914)
                     /locus_tag="O2692_002183"
     CDS             complement(9564..9914)
                     /locus_tag="O2692_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HCZ4299798.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     ILNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTDG"
BASE COUNT         3088 a         1720 c         1420 g         3762 t
ORIGIN      
        1 aattaatcaa atatgttttt tagattaata aagttgtaac tgtactattt tgtagtgtat
       61 ggtaatttat ttggatgaaa tataattctt tatactctat tgattaatca aatatgtatc
      121 tatcaaaatt cggcttattt attcactctg acatatccaa atacgcaaaa agactatttc
      181 taccttgtat cgatagaaat agtcttttta tacttaattg gtttaatttt tcataaaatc
      241 ttatactttt taaaaatggg atgaaatata ttttcctgaa acatgtaatt catgttcgtt
      301 acaaattgat aaaatatgat taagcgttgt tgcaaatgac attttctcaa aaagcgcttc
      361 actatcaatt ggtacttgtg tgtgaatttc agccagtcgc ttcgataaat atagttcatc
      421 taaattgtca ttaatcttat tacgttgccc agctgataat gcatcaatgt tttcaaccac
      481 attttcaacg ctttgatatt gctgaattaa cttaatagcc gttttctcgc caataccttt
      541 aacacccgca tatccatctg ctgtatcacc cataaacgct ttaatatcga ttagttgttg
      601 tggttcaagg gcatattctt cgttaaaacg atgtaatgta tatctattat aaatgttaaa
      661 acctttttta attagccaaa cttcaacatt gtcattaata cattgcagta aatctttgtc
      721 gcccgtaata atatagacat cgttatcagt tgaatattgt tgtgctaatg tacctataac
      781 atcatccgct tcatagtttt taacgccaat atttacaaag ccaaattgct ctgaaatttc
      841 tttaacataa tcaaattgtg gtatcaattc ttctggtggt gcagaacgat tttgcttata
      901 accatcaaac atatcatttc taaaagttga ttgtcccata tcccaacata cagctacatg
      961 tgtagggcgt atttcatgta ttgccgaaaa gatatgacgc acaaatcctt gtattccatt
     1021 tgtaggttca ccttgtgaat tgtacataaa ttgtttatga agacttgtag catagaaatg
     1081 tctaaataat agcgccatac catctacaag taatatttta ttaggcattt aaaacgttcc
     1141 ttctttctta atataaaata gttttcagtt gttgatgttt atctttcaat tgattgataa
     1201 gtgttgtatc taaatcaaat gctaataaat cgtttatttg cgtttgaata ttagcttcaa
     1261 aactttcaaa ttgcgattca gcttctacag ccatttgctt tacatataat tcaagctgtt
     1321 gcctcaataa tcccatttgt ggttgtaata attctaacgt actttgacaa attgattcat
     1381 gtatatctct ttgcccattt ggattcaaaa ttttacgttt tgttaattgt ttaggcaatg
     1441 catttagcat atcgttgaaa tcgatgtgca ataatggttg ctctatatta gctgattcaa
     1501 agttaaactg aggattaata atgacatgta aatctgctaa ttgttgaacg attggtgcaa
     1561 tttgctcagt gagttgctta ttaaagtatt tttttatacg ttctgtaatt aaagattgct
     1621 ctaaaaacaa tcgttgatga atttgatcta agtatactct cgtagacact ttcttttctt
     1681 cattaaaatc actattttgc gtcatttgag aattaaacac tgatttaaca tcgtcaagta
     1741 gttgtagttt taaacgggca tttaaatgat aaacttgttc ttcaacttcg ttgtctgtat
     1801 gttgtaacgt cgcatcaatt aattgatgtt gtaaacgctg tttatcttta taatctgtta
     1861 acctttgttg gcgacgtgaa atatcagctt tatttgtttc aaattctgta atcatctcta
     1921 cataagaacg atccatttgt tgaagctgat gaatcatttg ttgttctaaa attgatttag
     1981 attcaacatc aacaaattgt tgtatgcttt gttttaattg atcaatgccc ttatcttcag
     2041 cttgcaatgc atttcgactt gatacagcaa aaatgtctga ttgtaagtgt acttgtctta
     2101 atgcatctga tacatatgtt tcaactgctt caagatcatc ttgactttct gctaaatcag
     2161 cagcattaat taccattttt aatgcttggt tttcattcaa ctggttcata tctttcatgt
     2221 gttctataaa cgctttgtca ttatcagtaa atgaatgatt aaaataactt acatacaata
     2281 ttaagtctga agaagttaaa atttgttcgg tttcatttgt atgcctttgg ttatttgagt
     2341 gtagccctaa tgaatcaaca attattttac cttttaacca atcatgcatt aatgcaatgt
     2401 gtactgtctt aacaaatgtt gcatattcat cttctgcact ccactttttc aattcctgtt
     2461 gattaatggc atgtttttct ccattttcca acatattgac atacaattta taatgttttt
     2521 caactgcctg aataaaagcg agttgatttt tatttaaatg tgattttaac ttttctaaat
     2581 ctgaattaat aaagtcttct atagtcgaat aagacatatc ttggtattca actactgcat
     2641 taatttcatc taataattgc gattgtgatt ttagcgttat ataactctcg tctccataag
     2701 atatttctgt agtagctgct gtagtaggat ttggagaact gactaaaata tgctcgccta
     2761 ataatgcatt tatcaaacta cttttaccag cactaaatgt tccaaataca cctattttta
     2821 ttaatttatt atctatacgt gttaatgttt cgtggatatc ttgctttgta cgcttgaaca
     2881 aaggcacatc cgaaattata tcaagcgctt tttgaatatc tatagacata tttgttgtag
     2941 ctgtattttg atttagctgc tgattatcac gattatcctg agcattatca gtagccactt
     3001 gatatgttgt ctcttgtcga cctattaatt tatctagaga ttcatctaaa tgaatatagt
     3061 agtgacgata attcttagtc gtcaatgact gacgcagctc atttaattct gtataacgtt
     3121 gatattcttt taaatcatca ctttcttctg ttggtaattc atctgcctgc acattttcta
     3181 ttatttcttt aaaaattggt gttgattgtt gttcaacata tttcttaatg gctttaacca
     3241 cttcgtctga aaatgtaagt acataagtat tgctgattga tgtttgtggt tgatataaac
     3301 cttcaatcat ttctggctta acgtcataat gctgatttaa tactttatct gaagcttctt
     3361 ttttattgat aaaacgcgta acaaatgaca tatcttctcg cattggttga cgaatttgtt
     3421 gattaacatg ttcttgtaac gcatctgtcg ctgttaataa tcgctgttgt tgaatttgta
     3481 gttttttctt ctttttatta aaaaatccgc caacattaaa gtcttgagac atactttcca
     3541 aataacttcg taacatttct ctcatattat gcggcataat gtatgcattt tctaaaatat
     3601 ttttacgctt attctttaaa aatgccatta attcatctgg attatttaaa agttgtgcct
     3661 cttcactaat tgcttgatgt tgttgactat ttaaaaatgc ttgttcaaac tccgcttctt
     3721 cgatacctaa atcttctagt acttcctgaa tttcagactg aatgtaatct agctgagctt
     3781 cggtaatgta ttcaaccgtt cttgatgtat aatcctctat tgtctctcta tgttgatcta
     3841 atgaaattag ataacttgat aatacttcaa gttcattttc agggtgatca aatttagata
     3901 cataaaaggt gcgttctaat ttaatacccc aatctgcaat tgatttttca actcgagatt
     3961 taaacgtaga gaatgacaat tcatcgtctt gatgcttgtc aatttgatta atgataaaca
     4021 caacaggtat tccaacatca tttatatgct tcataaactt aaagttaagt tcagattgaa
     4081 cgtggttata gtcaaccgta taaaatatca tattacttgt atacatatat tgttctgtta
     4141 ttgactgatg tgatgcaaca tttgaatcaa cacctggtgt atcttgcaac gtaaacccat
     4201 tttcaaattt agctgattga aaattaattt ctacagattc aacgtcgaca ttttggcgat
     4261 tcatttccct tacttcatca taattagata atttggcata cgtttgattc ggcaaattag
     4321 caataatatc gtgattgtct gaaactgaca caatagcagt attacttgtc gttggtacag
     4381 gtgaacttgg taagatatct tgttcaatta ataaatttat cagtgtcgat ttacctgcag
     4441 aaaaatgtcc aacgaacgaa catgtatatt gctgcaaata tactttctta attacttggt
     4501 taattgtatg taaaagtgct tcatttcgcg acttttcaac ttcttttttt aatttatata
     4561 aaagatctaa ttgttcttta ttaatcatgt tactttcccc tttggttcgt tcacatagaa
     4621 atgcttgctt gttacaacga attcatacaa tatcgatgtc catttaaaca tagatcgtta
     4681 aatggctcgc attgtattat gtttaaattt tatcatagct gcttgataaa acatacgaat
     4741 aatcaatttt cttcaaacat tccaacttaa aagttcattt ttctaaaaat cacaaatcat
     4801 cgttaaattt ttctagaaaa taaatattgt cacagtttgt tcacacacat cacagaattg
     4861 acaccgtata atagccaatt gggagggaaa tatgaaaaat acatttaatc cattacaaag
     4921 attacatttc tatgcagcaa tatttattgc tccactgtta atcactttaa ccatttcggg
     4981 cattggttac ttgttctttc cagaagttga aaataatatt tataagaatg agttttttgg
     5041 tgacagtgat gtaaaaacgc atcaaacatt aaatgatgca gtacatcaag ttgaacaaca
     5101 atatgaagga ttctttgtaa gtaaagttag catacttgat gaaccatata acaaacgaat
     5161 tacactgagt gatatggcag gaaatcaacg ttacgtcttt ctagatcata acaatcaatt
     5221 tgttgcagat caaaatgcga aacatacgta ttctaatgtg atgcgaagta tacatagttc
     5281 tttgtttact gaaaatacta ttattaatta tttagtagag ttaaccgcat gttggatgat
     5341 attcatgatt ttatctggta cttatttact cattaagaag catttaattt ctaacaaaag
     5401 taaggcactt cgttggcaaa agtggcacgc aatgattgga gttatcattg caattccagt
     5461 atttgtatta gtcttaactg gattgccatg gtctggtttt atgggcagta aaattgccgg
     5521 tatgatggac acaaacggtg cccttggtca aggtgaatta gcgattaatc cacctaaatc
     5581 agatttgaac gaattacctt gggctacacg taaaaataaa cagccagctt catccgaaaa
     5641 aggttcaagt ggtcatcatg gtaatgcagc aatgcctcaa accaaattag attatcaaat
     5701 atctattgat aaggtcgttg aacaggcgca aaaagctggt attaaaaagc cgttttcaat
     5761 cgtatatcca agtgataaaa atggtacctt tattgtatct aatactagta attcaggtgt
     5821 tactgggcta gatgtatcac catacaagga acaaacactt tatttcgatc aatatagcgg
     5881 taaaaagcta ggtacgatta aatatgatga ctacggtatt attgctaaat ggtttacatg
     5941 gggcattccg cttcacgaag gtcatttatt cggcatttta aataaaatca ttaatttatt
     6001 tgtatgtatc gctttattag tagccattgg catggggttt gtctcttgga taaagcgtac
     6061 aaaaaatact gcaataaaag taccacatcg cgtaaaaaaa ccagcatcta tatcactcat
     6121 aatatgttta attgtattag gattattaat gccattattt ggattatcac ttatccttgt
     6181 atttataatt gaattaatat tatatattaa agatcgtcgt gctaaacaat aatgcactta
     6241 aagttttgaa ctgacgaaat ttacaaaatg attctcgtct ctctaattac ttaaaacggg
     6301 ttctataata aatcgtactg atggaaagtt tttacttttt atctgtccga ttttttgaat
     6361 ttgaagataa aaaaacatct aaaacgcata agaagataac tctacttctt caaaattacg
     6421 ttatagatgc ttttttatta attcatatgc atgtcgtgaa ttttcatcat taaaccgtgt
     6481 gggacgtcat catgtttaac aatctcttgt ttataaaatg tcttcccatc atctttagaa
     6541 actaatttta caaagtctcc ttttttcggc ttgaaatcat tagatggttc tactttgata
     6601 ttatgtttga ctaatccatc tttttcactg acaacttttt ctgcatttgt attactattc
     6661 atataaccaa aataagtcgt tttttgacta gaaaaaatca ttaaacatac aacggcaata
     6721 ccaataacaa ccaatgcaat agataaacct agacccattt ttttacccat ttatagactc
     6781 cttcattttt caaaaatacc tattatcatg agaacttaat tattactaat tttagtcacg
     6841 cattagaaac actttagttt tcaatagttt tacgttctgt ctttttaccc cagtattgat
     6901 aataagtaca ttctatatat ccattaaata acttacgtcg ctttgttgct ttacgatcta
     6961 ctaaatattc aaattcttta ttacttgtta aaatgtatgt agataaaaat ggatgttgtt
     7021 tcattagttt accaatataa cggtacattt cttcaacttc ttcacgatca ccaatacgtt
     7081 caccatatgg aggatttcca attaacgcca ccggttcttc tgtatcaatt gttaatgtat
     7141 tgacatcttt tacactaaat ttaataatat cagacaatcc aacttcttca gcgttacgct
     7201 tagcaatctc taccatttct ggatcgatat cagaagcata tacttcgatt tctttatcat
     7261 aatcagccat cttatccgct tcatcacggt aatcatcata aatatttgct ggcatgatgt
     7321 tccattgctc tgatacgaac tcgcgattaa aaccaggtgc gatattttga gcaattaaac
     7381 aagcttctat agctattgtt cccgaaccgc aaaatggatc aattaaaggt gtatcacctt
     7441 tccagtttgc aagacggatt aaacttgctg ccaacgtttc tttaattggt gcttcacctt
     7501 gtgctaatct ataaccacgt ctgttcaaac cagaacctga tgtgtcgata gtcaataata
     7561 cattatcttt taaaatggca acttcaacag ggtatttggc acctgattca tttaaccaac
     7621 ctttttcgtt atatgcgcga cgtaatcgtt caacaatagc tttcttagtt atcgcctgac
     7681 aatctggcac actatgtagt gttgatttaa cgcttctacc ttgaactggg aagttaccct
     7741 ctttatcaat tatagattcc caagggagcg ctttggtttg ttcgaataat tcgtcaaacg
     7801 ttgttgcgtt aaaacgtcca acaacaattt tgattcggtc tgctgtgcgc aaccataaat
     7861 ttgcctttac aattgcactt gcgtctcctt caaaaaatat acgaccattt tcaacatttg
     7921 tttcatagcc taattcttga atttccctag caacaacagc ttctaatccc atcggacaaa
     7981 ctgcaagtaa ttgaaacata tatgattctc cttttataca ggtattttat tcttagcttg
     8041 tgttttttat acatttccaa caaatttaat cgctgataca ttaacgcatc cgcttactat
     8101 tttaaaacaa ggcagtgtca ttatatcaag acaaggcgtt aattttaagt gttcttttca
     8161 tgaaaaaagc tctccatcat ctaggagagc taaactagta gtgatatttc tataagccat
     8221 gttctgttcc atcgtactca tcacgtgcac tagtcacact ggtactcagg tgataaccat
     8281 ctgtctacac cacttcattt cgcgaagtgt gtctcgttta tacgttgaat tccgttaaac
     8341 aagtgctcct accaaatttg gattgctcac tcgaggggtt taccgcgttc caccttttat
     8401 atttctataa aagctacgtc actgtggcac tttcaaatta ctctatccat atcgaaagac
     8461 ttaggatatt tcattgccgt caaattaatg ccttgattta ttgtttcatc aagcacgaac
     8521 actacaatca tctcagactg tgtgagcatg gactttcctc tatataatat agcgattacc
     8581 caaaatatca cttttaaaat tataacatag tcattattag taagacagtt aaacttttgt
     8641 atttagtaat tatttaccaa atacagcttt ttctaagttt gaaatacgtt ttaaaatatc
     8701 tacattattt gaagatgtat ttgttgttgt attattcgaa gaaaaacttt tattgtcctg
     8761 aggtcttgat gttgctacac gtagtcttaa ttcttctaat tcttttttaa gtttatgatt
     8821 ctcttctgat aattttacaa cttcattatt catatcggcc attttttgat aatcagcaat
     8881 aatgtcatct aaaaatgcat ctacttcttc tcttctatag ccacgagcca tcgttttttc
     8941 aaaatctttt tcataaatat cttttgctga taatttcaat gaaacatctg acattttttc
     9001 cacctcatta gaaactttga tcttcagacc actgtaagtc attgatgaat gctgttaatt
     9061 catcgaacgt cacaatatca caagtatagt ttgttttatc cataaaatca actaacatct
     9121 gcttgaagaa cttaggactc gcctcttgtt cctcatcata aatgagcaat gtttgatccg
     9181 aatgttcaag cataaattga tctgcttgtt taaattgaaa aggaccttga tacgacgtat
     9241 gaaaaatact atcaacataa tctgcatgct taattatgtt ggcatactta ctttgattat
     9301 gttcattcca cttttctgta tgtccttgga acggtgtaat tacagcaaac tttaacgaat
     9361 catatgttcg ttgtaattca ataacaactt ctgcagtcca taattctata cccatttgcc
     9421 cttgtattaa cacccattct aatccttcat ccaacagttg ttcaatttta tgttttataa
     9481 attgttttaa ataatgtact tcaggtgcgt catctttaaa aatgtttaat tcgaatgatt
     9541 tgtaacctgt tacataaact gttttaacca tcagtaccct cttttgttac gccatctaat
     9601 atattttgta aatcatattg aacactttta agcttttcta taaataactt tcgacttgtt
     9661 cgcttaaaat gacattcgac agaaagttgt tcaatgttag caatcagtag ctcaaatttc
     9721 cttgaattca tataaggtac ttctataata aattcacgat gtaatttgat ctcattgagt
     9781 atgctatcaa tatgttcagt atatggcttt acagtttggt aaaaatcatg atcttgttgt
     9841 tgtgatttca cattttcaaa attttgttgc atgttgttaa cttcataaat tagtgattcg
     9901 actacatcat tcatgaggac gcctcctaca ttttttaatt tatcacaata tactgtattc
     9961 gtcatgtttt aacactctat ataatttgat
//
LOCUS       DALAGF010000096         9976 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.096, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000096 DALAGF010000000
VERSION     DALAGF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9976
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.096"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(134..319)
                     /locus_tag="O2692_002184"
     CDS             complement(134..319)
                     /locus_tag="O2692_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299799.1"
                     /translation="MLSNKRVEKEAGCEHKKFLKKLLTLNSKVDNMIKSSKTTQLFLK
                     KNFKSVKLTIEITIKTI"
     gene            complement(408..1472)
                     /locus_tag="O2692_002185"
     CDS             complement(408..1472)
                     /locus_tag="O2692_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HCZ4299800.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            complement(1620..3062)
                     /gene="lmrS"
                     /locus_tag="O2692_002186"
     CDS             complement(1620..3062)
                     /gene="lmrS"
                     /locus_tag="O2692_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HCZ4299801.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTATGLI
                     IDQASWRVPFIIILGIAILAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALILSMVIIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            complement(3384..3857)
                     /gene="sepA"
                     /locus_tag="O2692_002187"
     CDS             complement(3384..3857)
                     /gene="sepA"
                     /locus_tag="O2692_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HCZ4299802.1"
                     /translation="MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            complement(3956..5299)
                     /locus_tag="O2692_002188"
     CDS             complement(3956..5299)
                     /locus_tag="O2692_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4299803.1"
                     /translation="MRLKSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLIS
                     LIFTAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRV
                     IQGFGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWH
                     WLFYINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQL
                     LLLNFLSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNL
                     YIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVS
                     SIIISFGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNL
                     GASIGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
     gene            complement(5476..6159)
                     /locus_tag="O2692_002189"
     CDS             complement(5476..6159)
                     /locus_tag="O2692_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCZ4299804.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            complement(6178..7365)
                     /locus_tag="O2692_002190"
     CDS             complement(6178..7365)
                     /locus_tag="O2692_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HCZ4299805.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVRVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDKN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            complement(7409..7924)
                     /locus_tag="O2692_002191"
     CDS             complement(7409..7924)
                     /locus_tag="O2692_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCZ4299806.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            8101..8361
                     /locus_tag="O2692_002192"
     CDS             8101..8361
                     /locus_tag="O2692_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299807.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            complement(8493..9863)
                     /locus_tag="O2692_002193"
     CDS             complement(8493..9863)
                     /locus_tag="O2692_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCZ4299808.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
BASE COUNT         3555 a         1861 c         1320 g         3240 t
ORIGIN      
        1 atagttacgt ttttggaatt aacgttgaca tattgtcatt cagttttcaa tgttcattaa
       61 tgttcaatct cttttattct acttcattta tttcttgaag tcaataactt tttgaacgat
      121 tactttattt attctatatt gtttttatag ttatttcaat ggtaagtttt acacttttga
      181 aattcttctt taaaaacaac tgcgtcgttt ttgacgactt tatcatatta tcaactttgg
      241 aatttaaagt caataacttt tttaaaaact ttttgtgttc acaacccgct tctttttcaa
      301 cgcgtttatt gcttaacaca agaacttatt ttaccagcat tccaaaacaa atcaacataa
      361 aaacgtacaa aataaaagta attttgtacg tttagcatat attataccta tttatttgta
      421 gcagctataa ctttttgtgc aatcgagcta taaattttac ctagacgatc atctgattga
      481 tatattgacg gtgcaaaatc ttttggattc caagatggtt gctctaaagg taattcccca
      541 agtaattgag tattaagttc atcagctaac ttagtaccgc cacctttgcc aaagacatat
      601 tctttattac ccgtctcttt actttcaaaa taactcatgt tttcaattac gccaagaata
      661 gaatgatccg tatgttttgc catcgcacct gcgcgagctg caacaaatgc tgctgtagga
      721 tgaggtgtcg ttacaataat ttccttactt gaaggtaaca tcgtatgaac atctaaagct
      781 acatctcctg ttccaggtgg aagatcgagt attaaatatt caatgtctcc ccatttaact
      841 tctgtaaaga aattcgtcaa cattttacct aacattggcc ctctccatat aactggcgca
      901 ttttcttcca caaaaaaggc cattgatata actttaacgc catgacgttc aactggaatt
      961 acttccttcc ctttaattcc aggcttttca tcaataccca tcatatctgg tacactaaat
     1021 ccatatatat cggcatctac taatccgact tttttccctt cacgagctaa ggcaacggct
     1081 aaatttactg caacagtaga tttaccgaca ccacctttac cggaggctat tgcaataaat
     1141 tcgactggat tattttgaga taataggcct tctatcgttt taggtttatt ttcttctttt
     1201 ggtttaaatt gatttacttt ttcttccggc aatgtttcaa atcgtatacc gaccgttttc
     1261 gcaccgtttt cttttaatgc attaacaaca gccatctgta aatctaattg cggtgcacca
     1321 cctaattgtg ccattgctaa tttaacactc acatgttctt tttcttcctt aatagaaact
     1381 tcaacaatac cttctgtttc ttttaaaggc acatctataa taggatcttt aatttctcct
     1441 accaattctt tcacttgatc taccgttaac aaagcaaatc ccccttaatc attttaagac
     1501 atctacatca tagcaaataa caatttttgc ttagtctatc actaaaagta taaaactttt
     1561 tattaaataa aaaaagcagt tagcctatta ttttaaatag accaactact ataattattt
     1621 taaaatttcc ttctattact ttctatttct ttaggttctc ttataaaaag tgctatcaca
     1681 taagctgcca taacaagcac agctacaaaa gcaaatgaga tatccacccc tatagcaatg
     1741 gattgctgtt taatttcagt tgcagttaaa tgcgatggcg atgtagataa aaatattttt
     1801 gcaccaaaac tcattaatgt aaccattaaa gctgtgccta gtgaaccagc cattacacga
     1861 ccaaagttca taatagccgt gccatgtgag atttcttcat ttctcaaaga attaattcct
     1921 gtagtattta tcggcatcat gagtaaagaa actgaaaaca ttctaattgc atatacgact
     1981 attaaatacg tataagatgt gtctgtatgc aagaaacaca acataattgt agtaattgtt
     2041 aaaattgtaa aaccagtata aatcaatggt ctaggaccat acttatcata aaatttacct
     2101 gtaaaaactg acataatacc atttattatt gcaccaggca tgataacaag tcctgataac
     2161 aaggcagata aagataaact gttttgaaca tatagcggta taagcagcgc aggtccaacc
     2221 attgacatca ttataatcat tgaactaatc gtacataatg caaatgttct atttttaaaa
     2281 acccttaaat ttaaaagtgc attagtaata ttaaattgtc tacgtataaa taaatagata
     2341 ataaccatac ttaatatcaa cgcacctatt actattggac ttgtaaatcc taaatctcct
     2401 gcactactaa atgcgtatag cattaacccg aacccaatag ttgaatacat tactgaacgc
     2461 ttatctaatt tcgtatattt cacttcattg taactcgaga ttgaaaccaa accgaaaaca
     2521 aaggcaagta tagcaattcc taaaattata ataaatggaa ctcgccaact cgcttgatca
     2581 ataattaatc ctgtagctgt aggtcctatt gctggtgcaa attgaattac taaacctgct
     2641 agtcccattg caaaacctcg atgttcttta ctgaacaatg taaataacgt aaattgcatt
     2701 aagggcataa gtacgcctgc acccatcgct tgaattacac gagctaacat taaaactcca
     2761 aaattcggag ctaaggctgc aacaatagaa cctaataaaa atgtccccat cgccgctaag
     2821 tataaaggtc tagttttaat tctatccatt agatatgccg tcagaggtat catgacgcca
     2881 ttaacaagca taaacccagt aactagccat tgcgatgtac tttcattgat atttaattct
     2941 ctcattatac taggtaacgc tgtatttaat aatgtttgat ttaaaatagc tacaaacgca
     3001 ctaatcaaca taacagcaac tatgaagttt cgtctccggg ttgttaattc aactttagcc
     3061 atcataattc ctccttttta aaacagatat acacttgtcc ctttaatacg tatagccatg
     3121 aacataatgg gagtagtact caaataataa agaagcacta cataataaat cattattggt
     3181 tcttacacat taggcacagc taatgtgtac ttaaaaacta acaatataac attgtagggc
     3241 ctaataattt attttatgtc tcagactcat acaatggtta tttaacttat ttaccacaaa
     3301 ttgagcacta agactcacta ttatcgattc ttctattgaa caaaaagaag ttggaacaca
     3361 tatgtgccct caacttcaga tatctatttt ctattattta aatttttact ttcagggtaa
     3421 aattgaagct tacaaacata taagaaccct ataataacca tgatgaccca aaaatcgata
     3481 ttgatataca catagttcag atgaataata aaataaaata acgttgttgc aactgtgtaa
     3541 gaaatcaaat gaaaagtcgc gcctctaaaa tatgcatttt caggcaacat ttttctaaaa
     3601 taatccatta aatgctcgac tgcaaatatg atgaagtacc caaagattat ataactgcca
     3661 taatataata ggttatcgta catacttcta ttatatgtaa agtcacccaa cctaaaataa
     3721 ataagtattc tacttaatcc atataaacca aaccctagaa atgttaaaaa aatcgcacca
     3781 gaaagtacaa aaataaagag aacaatcatt gtagttaaca agttataaaa cttatgttta
     3841 agataattca caatcattgg cctccttatt aataaaatat tgaacgattt atctagtatc
     3901 taggaaattt attatttctt actttaatta cactacatca attttaatca taattctatt
     3961 cttttgattg agatgactgt tctttaaaaa atacgaccca taaaacaata agcccaatgc
     4021 taattacagc aacagcactt aacacattgt gtaagtttgg accaaagatt cctgattgta
     4081 tcgcataaag atatcccatt attgttgaac ctattgatgc cccaagattt ttagttaatc
     4141 cataaaatga catcattttc ttcatttgta atgggctagt ttcctcttgg acaatcacac
     4201 tatctttcgt ataaatatat ccaaaactta atcctgccaa aattaacaca aatgctataa
     4261 gtaccggcaa tttaatacca aatgaaatga taatactact tactagtagc aatgtaaatg
     4321 atagtaagta aataactttc cttgatagtt ttgcttcaat tcgatgtaaa ttaaaattca
     4381 atgtaatcca agctacagat aaaggaaaaa taaccaatcc actttgcaat ggagataatc
     4441 ctagttgttc ttgaaggtag actggaatat ataaattgaa tcccattaaa caaatagctg
     4501 ttagaaggtc agttataaaa actaaagtaa tcgaacgatt aaattcaacc acaggtaaaa
     4561 atggtgaaga aacatgtttt tctactttaa ataaacacat tgccacaacg atagctaaga
     4621 taaaactcaa gaaatttaaa agtaacagct gttggtttaa taaagcaaac ataatcaatc
     4681 caataaatac ataaaatagc gtgagccctt tagtatcaaa ttttgattta gcaactgttt
     4741 cttcctctgg aaaatgaaat gtccatataa ctaatataat ggcaatgatc gcaatcggta
     4801 tatttatgta gaataaccaa tgccatgttg caaattctaa tataccacca cctagaagtg
     4861 gcccaatgat actcgaaata ccccaaacac tgccaactat gcccataatt ttatatcgta
     4921 acggaatttc aaatgctaac tttggaacaa tttgcgataa tgacatcaat acacctgatc
     4981 caaaaccttg tatcacgcgt gatataatta gcatagtaaa attagtactt aaaccacaca
     5041 taaaactacc tatactaaat aaaagtaatc ctgcaattgc cacataaata attttaaatc
     5101 tcgaaagtaa ttcgcccact ataggattgg caattactaa tgcgataaaa tacgctgtga
     5161 aaattaatga aattaaattt cctgcattta aatcttgttt aattgtaggt aatgcaagcg
     5221 aaataataga tgattcaatc gctgacataa acataattaa tattaaagct ataacagtta
     5281 tgattgactt taatcgcatg aaatacctcc ttctatatat ttatttataa gattttattt
     5341 acaaataatt ggttttcata tgtataaaca cttttgactt tcaaatctta acgataattc
     5401 tattacaata caatccccta ttagaatgat ttatgtaata aaaaaagcgg agtttccccc
     5461 agcttttcta aacgactaca taaaatataa gattgcaatt aaatgcaata gtgatgctat
     5521 tacaataaaa atatgccaaa tcatatgaaa atatggtcta ttcttttgtg cataaaacca
     5581 tgcaccaatt gtataagaca caccacctaa gaaaatgaat aatatgaata tccatgatgt
     5641 gcgaataaaa ataattggta acaagataat acctacccag cccatcacta aatagacaat
     5701 taagcttaac ttttggttaa ctttgacagc tatggattta tataaaatcc cccaaagtgt
     5761 cgttccccat aataaaataa atactgtcca acctatccaa ccacctacaa ctgttaacaa
     5821 aataggcgta tatgtaccag aaattgctac gtaaatcata ctgtgatcga taatccttaa
     5881 aatatattta tgaggtgttt cattttgcat agaatgataa atagtagatg aaataaacat
     5941 cataaagata gaaataacat aaatagacat actcacagac gcaagcgtac catgtgcaat
     6001 ataactgtgc acagccgcat agggtaacac taataatgtt aacaaagctg caacaccatg
     6061 agatgccgca ttaccaattt cttctccaaa tgataaaggg atgatatcct taaatgtttc
     6121 tacgattgtg tcattacttt ttttagttga tttggacatc atagtcaccg cctatcatta
     6181 aattaaaccc attctacgta aatcttctgt tgcagttgca acactatctt taccttcttt
     6241 atttttaaga ggtgaaaatt cttcttctct aggaacttgt aacgtgacaa aactagttcc
     6301 ataagtaaat atgtcaaaat agaacaattc gaattttaaa gttggttgtt caataacacc
     6361 aaagttctta tctaattgct tcaaattctt aatagctaaa tgataaggta actcacgatt
     6421 tacagcgttt aaaataaaac caagtttaaa tgcatgaata ccgatattag cattgttaaa
     6481 ttgatttgca acttctggat ctaattctga atactccagt actgtatctt tacaatcgac
     6541 attaactaaa cgtccaacac tttctcctgg ttttggttga atcgttttag aagtaatatc
     6601 gtaatcatgt tctaccgtaa atcctgcaaa tagtggatcc aacactctta ctaaaacatt
     6661 gtcgatatta ttcaagaaaa tatacttaac gccattatta gacatttctt ctaagtatcc
     6721 tgccttgtct aaagatttaa acacgccacc attaccattc ggcgtttcca taatacgacc
     6781 ctgttgattt aaaattaact tcccttcttc actaagcgcc acaatattat cttgtttaaa
     6841 gaaatggatt gcttcttgat catatccaaa ataactatgt gcttcgaaat aagctaaagt
     6901 ttcttcatga ttgatatcac ttgtcataat ataccattga atcgtatgtc cagattgatg
     6961 attcaatgtt tttaattggt tagcttgaag ttcaaataaa cttacacctt caatttcaaa
     7021 cgaaccttta gggcccttgt aaccaagtct tgttccttga ccacctgcca tcaaaagtac
     7081 tgcaaattgt ccttctttaa tagcttgaag tccttgttcc tctaaacgct taatctcatc
     7141 atcactcatt tgtgatttaa tatcatattt cacttcagaa actgcagata catcatctat
     7201 tgttttctta ttgatgtata aatcgttata taatttggca ataaaatcta aatctaatga
     7261 cgctaccttc tcttcaagtg cttctttttc attgttactc attatttttt catattcaca
     7321 caaatgatct tgtttatatt tagctaattg atttttatct agcattttat gcctcctgct
     7381 gagtttgtaa cctttaataa tttattttct ataaaaactt agtattccag ttgcttatta
     7441 tatcattgat gaaaggctga aataaaacat aaactgttcc gaccattaac gctgtagcta
     7501 aagataagtc tacaagtcca cctgttttaa attgaatcgg tgtcttcaca ttaaacggta
     7561 acggataaaa taatttcacg ccttttggtg ttatcatatc tagtataaca tgtgacacta
     7621 atccaattat aattgctgcc atataataat taggcgtctg aataatttgc aataaaaatg
     7681 ctatgattgc gataaataat attgaatgcg taaaggttcg atgtccaaag atcaatctca
     7741 caaaaaaact aattacctta aaccttctgc caatcttact ttgagtgtga catatatcgg
     7801 gtaataagct agctagagtt gctagaatga taaccgtaac cgacgaaaaa atatccgttt
     7861 gaaaatattg tgttgttagc gctccaacga gcatgccgca tgaagcatgt gttttacctg
     7921 tcatatttgt tctcctttaa tactcacatt ttaccacatc cctaacaaaa acacgaacat
     7981 attttcgggt taaaattcat tagtatgaca caatttaaaa aagtatcaca taactcttga
     8041 aaacgattac aaaatcgttt atgatgtatt tacaaaatat ttaaaggatg tgtttgaata
     8101 atggcaatga cagtaaaaaa ggataataat gaagtgcgta ttcaatggag agttgctgat
     8161 atcaaaattc ctacaagtga aattaaaaat attacacaag accaagatat tcatgcagtt
     8221 cctaaattag acagcaaaga tgtatctaga atcggctcaa cgtttggtaa aacgaatcgc
     8281 gttattatcg atactgaaga ccacgaatac attatttata ctcaaaatga tcaaaaagtt
     8341 tacaatgaat taactaaata aattgtataa aaaaatcatt catggtgagg gcttcatgaa
     8401 tgattttttt aattgattca acacccagca taaacaaata caaaaggaca actgttccca
     8461 taattttaac agttgtcctt tttcacatat atttataaca aaagatgtgc catcaaagaa
     8521 ataattggta gtgtaatgat tgttctaatc aagaaaatca taaacaattt gccgatgctt
     8581 acaggaatct tcgaaccaag tatgacgcca cctacttcag acaagtatat taactgcgat
     8641 atactaagtg ccccaataac aaaacgagtt atatcatttt gtacaccttc aattaatata
     8701 gaaggtaaaa acatatcggc aaaaccgata ataatcgttt gagaagcctg tgccgcttca
     8761 ggtatttgca ttaactctaa aaatggaaca aaaggtttac ccaatatgac aaaaaagggc
     8821 gtgtagttcg caataatggt agcaatagta ccaatactca ttactacagg caaaataaca
     8881 aaccacatat caatgactgt ttttaatcct gacttaaaaa agtcaataac gcccggtgct
     8941 ttaataccta cttctgttgc agtatcaaag ccatgtctca atgccgtctt tccttctggc
     9001 aatgcctcag tacgcgcact ttcaggtacc tccttagcat actcatcagg aattttattt
     9061 aaaggccaaa ttcttggcat aatgactgct gcaacgaggc aggatactat cactgataaa
     9121 tagaaagcaa aaaattgatt ttgcatgtgc actgtttcag caactacaat tgcaaaggtg
     9181 atagaaacta cactaaatgt cgttgaaata actgttgcct cacgacgaga ataatatcct
     9241 tcaccatatt gtctacttgt aattaagaca ccaacagttc cgtctccaat aaatgatgct
     9301 aaattatcta ccgtcgaacg tcctggcaat gtaaataaag gtctcataac cggtctaaaa
     9361 ataggaccca acatctctaa caaaccgtat tccattaata gcggtaaaaa tagagctgca
     9421 aataagaata ctgccactaa tgttggcaat aaacttgaga acaataatcc acccgtttca
     9481 tctgagtaaa taacctttga accaattcgt aaaaatgtca tccatgcaaa aacaactgct
     9541 aatattcgta aaattaacca accaattcta acgttaaaag cattgttcat tagcccgtca
     9601 ggtttcaatt tatcttttaa aatagttgaa caaatcagag ttatgatacc cgataaagta
     9661 attatcgtca caattaaaaa tggcattacg ccacctaata catctttaag cacgcctgct
     9721 aaaaatgcca cgggcaacgt tgtttgcttc tgtccatctt gttcgactgg aattggtact
     9781 aaaaataata agataccaat taaagacatc gtaataaact taagtctccc aataactatc
     9841 tctttccttg aaaagctatc cataaaatca atccatttct ctatgtattc gttttaagta
     9901 tatacagaat tctattcagt taacaaacat attccttatc attctatctt tcaaaatgtt
     9961 tatgtatgca aaataa
//
LOCUS       DALAGF010000097         9946 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.097, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000097 DALAGF010000000
VERSION     DALAGF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9946
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.097"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..704
                     /locus_tag="O2692_002194"
     CDS             <1..704
                     /locus_tag="O2692_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299809.1"
                     /translation="QYKKNVVLDKVYYSEVYKYSDNLIKVFSEDVWNIMGYKKYNEYF
                     TNRDDTTLKITNLYIGQSFGTEGNRNVIDRIGKGHEKLQEIMTYQPENRKTVLIFYKI
                     QPDNIYITNQYGKEAVSRLKRILEQSIPNKEYIDLSEISLITFFKPEYNKQHVNEEFN
                     SLSSSKIKNLAKENDGIIIYLNFKDVNYQLISEGNTFFSAKPYLHCVFNKDILNMDKV
                     DIENFFKSIEVKYSK"
     gene            1258..1440
                     /locus_tag="O2692_002195"
     CDS             1258..1440
                     /locus_tag="O2692_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299810.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSIYLMSIFL
                     ICLGTASIIYNYKTNR"
     gene            complement(1422..1661)
                     /locus_tag="O2692_002196"
     CDS             complement(1422..1661)
                     /locus_tag="O2692_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299811.1"
                     /translation="MCVVIMRDVGLIDVEDCYDIFGMWCLVNGGLKLSFYGGLKMLFY
                     VGLEFVVLWRYKKPQQDLDLTVTYTFHTYIYRLVL"
     gene            1894..3558
                     /gene="alsS"
                     /locus_tag="O2692_002197"
     CDS             1894..3558
                     /gene="alsS"
                     /locus_tag="O2692_002197"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCZ4299812.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            3595..4299
                     /gene="budA"
                     /locus_tag="O2692_002198"
     CDS             3595..4299
                     /gene="budA"
                     /locus_tag="O2692_002198"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCZ4299813.1"
                     /translation="MTNVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDKDFTK
                     ANIDYKDIADEIREAE"
     gene            complement(4663..5088)
                     /locus_tag="O2692_002199"
     CDS             complement(4663..5088)
                     /locus_tag="O2692_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCZ4299814.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTVDGY
                     HTVPYTISVDGITALHRTYFIFPENKNVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            5382..6197
                     /locus_tag="O2692_002200"
     CDS             5382..6197
                     /locus_tag="O2692_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HCZ4299815.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TAVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDSRILEALVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMRKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
     gene            6408..7262
                     /locus_tag="O2692_002201"
     CDS             6408..7262
                     /locus_tag="O2692_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCZ4299816.1"
                     /translation="MNKTNFGAYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTR
                     QSDVHELKVCTQNDGTIEQIWIDEANQFGVSVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEIFWGGDNELYNYHYLYPHQRYAIDLVIKENHKTYKNQGKVNTDYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTNESQFLGNYIVIKHAENEYSLIAHLHQYSI
                     IVNEGQNVKYGDIIGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFINNRELIKGD
                     VVCGLQAE"
     gene            complement(7322..9745)
                     /gene="hysA"
                     /locus_tag="O2692_002202"
     CDS             complement(7322..9745)
                     /gene="hysA"
                     /locus_tag="O2692_002202"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase HysA"
                     /protein_id="HCZ4299817.1"
                     /translation="MTYRIKKWQKLSTITLLMAGVITLNGGEFRSVDKHQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDKYDENNPDMKKKFDATEKEATNLLKEMKTESGRKYLWS
                     GAETLETNSSHMTRTYRNIEKIAEAMRNPKTTLNTDENKKKVKDALEWLHKNAYGKEP
                     DKKVKELSENFTKTTGKNTNLNWWDYEIGTPKSLTNTLILLNDQFSNEEKKKFTAPIK
                     TFAPDSDKILSSVGKAELAKGGNLVDISKVKLLECIIEEDKDMMKKSIDSFNKVFTYV
                     QDSATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDSTK
                     AKYKKIVKSSVESDSSYKQNDYLNSYSDIDKMKSLMTDNSISKNGLTQQLKIYNDMDR
                     VTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYHDNFWV
                     TADMKRLSGTTTLDNEILKDTDDKKSSKTFVGGTKVDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTNSDNQENNSVF
                     LESTDTKKNIGYHFLNKPKITVKKESHTGKWKEINKSQKDTQKTDEYYEVTQKHSNSD
                     NKYGYVLYPGLSKDVFKTKKDEVTVVKQEDDFHVVKDNESVWAGVNYSNSTQTFDINN
                     TKVEVKAKGMFILKKKDDNTYECSFYNPESTNSASDIESKISMTGYSITNKNTSTSNE
                     SGVHFELTK"
     gene            complement(9742..9909)
                     /locus_tag="O2692_002203"
     CDS             complement(9742..9909)
                     /locus_tag="O2692_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299818.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVDSHLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
BASE COUNT         3435 a         1452 c         1662 g         3397 t
ORIGIN      
        1 aacaatataa aaaaaacgtt gtattggata aagtatatta ctctgaagtt tataaatata
       61 gtgataattt gataaaagta ttctctgaag acgtttggaa cataatgggg tataaaaaat
      121 ataatgaata ttttacaaac agagatgata ctacattaaa aattactaat ttatatattg
      181 ggcaatcttt tggtacagag ggtaatagaa atgtcattga tagaataggg aaaggtcatg
      241 aaaaattaca agaaattatg acttatcagc cagaaaatag aaagactgtc ttaatatttt
      301 acaagataca gcctgacaat atttatatta caaaccaata tggtaaagag gctgtgtctc
      361 gattaaaaag aattttagag caaagtatac ctaataaaga atatattgat ttatcagaga
      421 tatcattaat tacatttttt aaaccagaat ataataaaca acatgtgaat gaggagttta
      481 attctttatc ttcttcgaaa ataaagaatt tagccaaaga aaatgatggt attattattt
      541 atttaaattt taaggatgtt aattatcaac tgataagtga aggcaatact tttttttctg
      601 caaaaccata tttgcattgt gtgttcaata aagatatttt aaatatggat aaagttgata
      661 tagaaaattt tttcaaaagt atagaagtca aatacagtaa ataagatcaa ttattgaatc
      721 caccgaccag cagatttttt taaaaaatta ataccccgtt aatacccttt gcttcaattt
      781 gatgaaaatc aatgaaattc aaaattgaag aaatccttta atatcaaggt gtacgacagt
      841 ctatataatc atgcgaaatt ctaaaatttt ctgaacgtaa aaaaacaaat tcttaaagca
      901 gctcgtcgtt cacctcaatt ctcaaaacgt taattgtcgg acgatatata tacaaaacac
      961 ctcgatgtta tgtcgaggtg tttttttgcg tttgtgtggg gaatatggaa tagagtataa
     1021 atgaattaat gtctcaggta tagaattaat tcaactatga attattgatt tacacgtata
     1081 atttgtacat gcttacacaa tcaaaagtgt acattattaa attgtcattt agcttgcgga
     1141 gaccgtacga tagtgttcta ataatagata ttcaagggaa agagctatcg gatgataacc
     1201 agatgataac tcaagaacca tgatttaaga gtgttatcaa aataaggagt gacttaattg
     1261 aaagattcta tattttggaa gaaagctttt attcctgttt attttattgt tgcgatgctg
     1321 gtgtttctac tttttaggtt ttatattaaa acagataact tttctatata tttaatgagt
     1381 atcttcttaa tttgtttagg aactgcttct atcatttata actataaaac caatcgataa
     1441 atataagtat gaaatgtata agtcacagtt agatctaagt cttgctgtgg ctttttatat
     1501 ctccataaaa cgacaaattc aagcccgaca taaaacagca ttttcagccc accataaaac
     1561 gacaatttca gcccgccatt gactaaacac cacatcccaa aaatatcgta acaatcctct
     1621 acatcaatca atccaacatc cctcataatc acaacgcaca aaatctattc atgcattttt
     1681 ggaatactta gtattacaaa taacgatttt tattcatcta acaaaggtta tataatgtac
     1741 tgaaggcaat ttttatgtat tacaaatcta atcgtacatg taaaattttg ataaacatca
     1801 ttaattttgc gtaactatca ttagattaca aatcacaaag taattacatg taatacacat
     1861 ctatacatca catttgaagg gaaatgaata taaatgactg ataaaaagta cactgcagcc
     1921 gatatggtta ttgatacttt gaaaaataat ggggtagaat atgtttttgg tattccgggt
     1981 gcaaagattg actatctatt taatgcttta attgatgatg gtcctgaact tattgtcact
     2041 cgtcatgaac aaaacgccgc aatgatggcg caaggtattg gaagattaac aggcaaaccg
     2101 ggtgtagtac ttgttacaag tggtcctggt gtaagtaatt taactactgg attattaacc
     2161 gcaacatctg aaggggatcc tgtattagcg ttaggtggcc aagtgaaacg taatgattta
     2221 ttacgattaa cgcatcaaag tattgataat gctgcgctat taaaatattc atcaaaatat
     2281 agtgaagaag tacaagatcc tgaatcatta tcagaagtta tgacaaatgc aattcgaatt
     2341 gctacttcag gaaaaaatgg cgcaagtttt attagtattc cgcaagacgt tatttcttca
     2401 ccagttgaat ctaaagctat atcactttgc caaaaaccaa atttaggagt accgagtgaa
     2461 caagatatta atgatgtcat tgaagcgatt aaaaatgcat catttcctgt tttattagct
     2521 ggtatgagaa gttcaagtgc agaagaaaca aatgccattc gcaaattagt tgagcgcacg
     2581 aatttaccag ttgtagaaac attccaaggt gcaggtgtaa ttagtcgtga attagaaaat
     2641 catttcttcg gtcgtgtggg cttattccgc aatcaagttg gtgatgaatt attacgtaaa
     2701 agtgatttag ttgttacaat cggttatgat ccaattgaat acgaagctag taactggaat
     2761 aaagaattag aaacacaaat tatcaatatt gacgaagttc aagctgaaat tactaattat
     2821 atgcaaccga aaaaagagtt gattggtaat attgctaaaa cgattgaaat gatttctgaa
     2881 aaagtggatg agccatttat aaatcaacaa catttagacg aattagaaca attaagaaca
     2941 catattgatg aagaaactgg tattaaagcg acgcatgaag aaggaattct acatccagtg
     3001 gaaattattg aatctatgca aaaggtatta actgatgata ctactgtaac agttgatgtt
     3061 ggaagtcact atatttggat ggcacgtaat ttcagaagtt acaatccaag acatttatta
     3121 tttagcaatg gtatgcaaac gcttggtgta gcattaccgt gggcaatttc agctgcactt
     3181 gtgcgcccta atacgcaagt tgtgtccgtt gctggcgatg gtggcttttt attttcatca
     3241 caagatttag aaacggccgt acgtaaaaat ttaaatatca tccagcttat ttggaatgat
     3301 ggaaaatata acatggttga attccaagaa gaaatgaaat ataaacgttc gtcaggtgta
     3361 gacttcggtc ctgtagattt tgtaaaatat gcagaatcat ttggcgcgaa aggtttacga
     3421 gttactaatc aagaagaatt agaagcggca attaaagagg gctatgaaac agatggtcca
     3481 gtattaattg atatacctgt aaattacaaa gataatatca aactttcaac aaatatgtta
     3541 cctgacgtat ttaactaaaa taaagataaa tgttaaagag gagtgggaga ttttatgact
     3601 aatgttttat accaacatgg tacattaggc acattaatgg caggattatt aaaaggaact
     3661 gcatcaataa atgaattatt gcaacatggt gacttaggta tcgctacact aacaggttca
     3721 aacggtgagg taatcttttt agatggaaaa gcttaccatg caaatgaaca taaagaattt
     3781 gtagaattaa aaggtgacga gttaacacca tatgcaactg taactaaatt tgtagcagat
     3841 acaagctatg aaacgaaaga taaatcttca gaagcagttt ttgcagaaat taaggaaaag
     3901 atgttgagtg aaaatttatt ttcagcagta aaaatttcag gcttatttaa aaaaatgcat
     3961 gtacgtatga tgccggctca agaaccacct tatacacgtt taatcgattc agctagaaga
     4021 caacctgaac aaactgaaac gtatgtcaaa ggttcagtcg ttggtttctt tacaccagaa
     4081 ttattccatg gtatcggatc agcaggattt catgtacact ttgcgaatga tgatcgtaac
     4141 tttggtggac atgtcttaga ttttgaagta gaagatgtta aagtagaaat ccaaaatata
     4201 gaaacatttg aacagcattt tccaattcaa gataaagatt tcactaaagc aaatattgac
     4261 tataaagata ttgcagacga aattagagaa gctgaataat gaatccagaa atataatgac
     4321 ggtttatgaa aattgacttc ataatgcgcg atttagaaat gatagtttgt aaatatgatt
     4381 aaccatgact acaatagaac aaatatattt ataattacgt ctaagtaata aaataaatcc
     4441 cttcactatt agcagtagtg aggggattta ttaggttcca gatatttgag atttgctgtt
     4501 atgtttagat tataaattgt ggtacacact catataaaat ttactattgt ataggccaat
     4561 ctgttactac gagaagcaaa caacaataat ttacaagttc aataactaaa aagacaaacg
     4621 ccaatttttc agcgcttgcc ctataaaact attttcaaat tattatttaa ctacaatttg
     4681 tatctgtttg attgtacttg gatcaattga atttttagcg tcgtcatttt tctttagatt
     4741 cactactttt ttattaccat cattcaaagt aagcgtataa gttgctgttt gggcattatt
     4801 aattttttct gttgtaacac cacgttgaga agctaattca ttttttactt tactgtcaat
     4861 ttcttgataa agaacatttt tattttctgg gaagataaag taagttcgat gtaatgcagt
     4921 aataccatct actgaaattg tgtaaggaac agtgtgataa ccatccacag tcatttcttt
     4981 atagccgtta ttactatctg cagatgcttc gtgactcggt aatgcagctc cgaccgttga
     5041 taataatccc aatgctaaag tggcagtaac aaatgatttt agtttcatat ctcaatctcc
     5101 ttttcgggtt gtatgatatt acactatttg tataacacgt ttcataaagt ttaaaacata
     5161 ctttttgcat tttttatatt ttaattttta gtaatagttt ttaacgaggg atatgactcg
     5221 tgcattttga aaagtggtaa gcaatgtttt tagtttatag ggattgatta gttgtatata
     5281 ataatcatga aaagcatata gtcgatctta tacttttaat tgagacttat agtgtattta
     5341 agttatagat aaattcatta aaatattatg ggggaatgag tatgtcaaag cgtctattat
     5401 tgtttgattt tgatgaaaca tattttaaac ataatacaaa tgaagaagat ttaagtcatt
     5461 taagagaaat ggaaaaattg ttggaaaaac taactaataa taatgaagtg atcactgctg
     5521 tattgacagg aagtacattt caaagtgtca tggataaaat ggatcaagtt aatatgacgt
     5581 ttaaaccatt acatatcttt tctgatttga gttctaaaat gttcacttgg aataatggcg
     5641 aatatgttga atcagaaaca tataaaaaga aagtcttgag cgaaccattt ttatttgaag
     5701 atattgaaga tatattacgt catatttctg cgcaatataa tgtcgaattt attccacaaa
     5761 gagcatttga aggtaatgaa acacattata atttttattt tcattcaaca ggtaatcaca
     5821 acaatgatag tcgtatccta gaagcacttg tcagatacgc aaatgaccaa aactatacag
     5881 cgagatttag tcgaagcaat ccattagctg gtgatcctga aaatgcgtat gatattgact
     5941 tcacaccgtc aaatgctgga aaattatatg ctactcaatt tctgatgagg aaatataata
     6001 taccagtaaa atcaatatta ggttttggag atagcggcaa tgacgaagca tatttaagct
     6061 atcttgaaca tgcatatttg atgtcgaata gtagagatga agcattaaag cagaaattta
     6121 gattaacaaa atatccatat tatcaaggga taacactgca tgttaaagaa tttgttgaag
     6181 gtaagtatga ttattgatta gttgagtgat atgtatactt tttaagtgca aatattaata
     6241 tttaagggat gattagaaaa tatggtgaat cgactacact ttagctttaa aaaattattt
     6301 atatgttgat ttatccaatt aagtgtagac gattcaggta gcgcaaaaat gacgcaggaa
     6361 ctggagaaag aattgatacc aaaattaata taataggtta agtatatatg aataaaacaa
     6421 actttggggc ttatttaaat aaaagtaaga gcaggaaaat atataagatt tttgatcaaa
     6481 atttgaagcg agagtttagt tattggcagt ttaggaaact tttcaaatct tatacaaggc
     6541 agtctgatgt acatgaatta aaagtgtgta ctcaaaatga tggtactatt gagcaaatat
     6601 ggatagatga agcaaatcaa tttggagtgt cagtgacttg gaaagataat agtattatcg
     6661 gatttgttat gcgtccgtta attaattatg atgcgattaa gaatgaaacg actttaaaat
     6721 acgcagtgcc gacaacagat tgctatgaga ttttttgggg tggagataat gaattatata
     6781 attatcacta tttataccca catcaaaggt atgctattga tttagttatt aaagaaaatc
     6841 ataaaacata taagaatcaa ggaaaagtaa atactgatta tttttgtttt ggaaaagata
     6901 tcattgcgcc agcaaatggt acagttgaaa aagtagtgaa tggggttcaa gacaacagta
     6961 ttggaagtac gaatgaatca cagtttttag gaaattatat tgtaattaag cacgcagaaa
     7021 atgagtatag cttaatagct catttacacc aatattcaat cattgtgaat gaggggcaaa
     7081 atgttaaata tggtgatatc attgggaagg ttgggaattc tggcaattct actgaacctc
     7141 atatacattt tcaagtaatg aatgataaga atattgaagc atgtacatct ttaaaaattc
     7201 gatttataaa taatcgagaa cttatcaaag gggatgtggt ctgcggatta caagctgaat
     7261 gatggcgata cttataaaat ctcgacacta taaaaatggt atagtgtcga gattttcttg
     7321 cttatttagt taattcaaag tgcacgccgg attcattaga agtcgacgta tttttgtttg
     7381 taatagagta accggtcatt gaaattttag attcaatatc tgaagcggaa tttgtagatt
     7441 caggattata gaagctacat tcataagtgt tatcatcttt ctttttaagt ataaacatac
     7501 ctttggcttt aacttcgact ttagtgttgt taatgtcaaa agtttgagtg ctattactat
     7561 aattaacacc agcccaaacc gattcattat ctttcacaac gtggaagtca tcttcttgct
     7621 taacgacagt tacttcatct ttttttgtct taaagacatc tttagataag cctggataca
     7681 acacatatcc atatttattg tcagaattag aatgcttttg agttacttca taatactcat
     7741 cagttttttg tgtatccttt tgacttttat ttatttcttt ccacttacca gtatgacttt
     7801 ctttttttac agttattttc ggtttgttta aaaaatgata accgatgttc tttttggtat
     7861 ctgtggactc taaaaagact gaattgtttt cctgattatc agaattggtt gtttgtttat
     7921 cgtctgtata taacgtatac ccattcgctt tgcgattttc aatcgttgta actggattct
     7981 ttgatgaatc agtactttta atgccagttc ctaagaagac aattttatcg tttaatatga
     8041 aatatgattt tttggcagtt aaagttttgt cctgattttc aaaatccatt ccgatactag
     8101 catgttggtc atcaactttt gttccgccaa caaaagtttt actcgacttt ttatcatccg
     8161 tatcttttaa tatttcattg tctaaagttg ttgtacctga taaacgtttc atatcggctg
     8221 tcacccagaa gttatcatga tagtgtttga catcgctgtt atataaataa gacattccag
     8281 caccagtgtg ccaacctttt aaattctctc cgttgatact ttcatagcgt gctacgtttt
     8341 tcgacgtcat acttaaacca aatgcaaagt ctaagtcttt gttatgatag gtgacacgat
     8401 ccatgtcatt atatatttta agttgttgtg ttaatccgtt tttagaaata ctgttatctg
     8461 tcattaaaga cttcatttta tctatgtccg aatatgaatt taaataatca ttttgtttat
     8521 aacttgaatc tgactctact gaagatttga caatcttttt atacttagct tttgttgaat
     8581 catccatggc atcacttaat ctcaacaatg atttcattac tgttgcagat gctgagtgac
     8641 tggtttcatt ttcacgactg atagctctac ctcgtgataa atccatcatt tcacctttat
     8701 aaatgagtgg cataaatccg tcgtcaatcc atgactttaa ggttgtatca ttttgggttt
     8761 tatcattaaa aggtgtttct tttatcatcg gcatcatttg agaaatcccc tctaagagta
     8821 caacgccata agcaccagtg tatggaacgt cttgatgatc aatgtaagag ccatctttat
     8881 aaaatccatt acgttcttta ccagtggcag aatcttgaac gtaagtgaag actttattaa
     8941 atgaatctat agactttttc atcatatctt tatcttcttc gataatacat tctaaaagtt
     9001 tcaccttaga aatgtctact agatttccgc ctttagcaag ttcagctttt cctacagaag
     9061 ataatatttt gtcactatct ggggcgaaag ttttaatagg ggcagtgaat tttttctttt
     9121 cttcatttga aaattgatca ttcagcaata taagcgtatt tgttaatgat ttaggtgttc
     9181 caatttcata atcccaccaa tttaagttgg tattcttgcc agttgtttta gtaaaattct
     9241 cacttaattc ttttactttt ttatctggtt cttttccata tgcattttta tgcagccact
     9301 caagggcatc tttcactttc ttcttatttt cgtcagtatt taaagtggtt ttaggattcc
     9361 tcatcgcttc tgcgattttc tcaatattac gataggtacg agtcatatga gaagaattag
     9421 tttcaagggt ttccgctcct gaccacaagt atttcctacc actttcagtt ttcatttcct
     9481 tgagtaaatt cgtcgcctct ttctctgtag catcaaactt cttcttcata tctggattat
     9541 tctcatcata cttatcataa ccatagttaa cgtccagcca tgtgttcctc aatttttcat
     9601 aatctggcgt ttgaacattc gtatcagcca cagcgatttg atgtttatca acacttctga
     9661 attcaccacc attcaaagta atcacaccag ccattaataa cgtaatggtg gataattttt
     9721 gccatttctt tattctatat gtcattgaca tgtctccttt ttgtgttgcg cgtgcgcaat
     9781 gaatattatg attaaataat gattcaattt ttcaaaattc gttaacgtat acaaatgact
     9841 gtctactgtc aaacaatcca caaagaatgt tgatgcatat aaacaatcga tcacccaaat
     9901 tttccgcatc acccaaaaat tgcaactaaa ataattttca aaaaat
//
LOCUS       DALAGF010000098         9842 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.098, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000098 DALAGF010000000
VERSION     DALAGF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.098"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            152..1447
                     /gene="purB"
                     /locus_tag="O2692_002204"
     CDS             152..1447
                     /gene="purB"
                     /locus_tag="O2692_002204"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCZ4299819.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            1556..1858
                     /locus_tag="O2692_002205"
     CDS             1556..1858
                     /locus_tag="O2692_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299820.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            2030..2722
                     /locus_tag="O2692_002206"
     CDS             2030..2722
                     /locus_tag="O2692_002206"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HCZ4299821.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKMAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            2719..4911
                     /gene="pcrA"
                     /locus_tag="O2692_002207"
     CDS             2719..4911
                     /gene="pcrA"
                     /locus_tag="O2692_002207"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCZ4299822.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            4915..6918
                     /gene="ligA"
                     /locus_tag="O2692_002208"
     CDS             4915..6918
                     /gene="ligA"
                     /locus_tag="O2692_002208"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCZ4299823.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            6931..8130
                     /locus_tag="O2692_002209"
     CDS             6931..8130
                     /locus_tag="O2692_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HCZ4299824.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGSTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(8219..9757)
                     /gene="putP"
                     /locus_tag="O2692_002210"
     CDS             complement(8219..9757)
                     /gene="putP"
                     /locus_tag="O2692_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCZ4299825.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIIRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
BASE COUNT         3707 a         1457 c         1833 g         2845 t
ORIGIN      
        1 agaatgagaa tatacggaaa ttttttatac atttataacc aattctgtta aactgtgaag
       61 agtagagtta aaaacgaact ttttgtttat ttttattaaa aaatgttctg tttttgaaaa
      121 ttcgagtata catacgatag gagttaaaaa aatgattgaa cgctattcta gagaagaaat
      181 gtctaatatt tggactgatc aaaatcgcta tgaagcatgg ttagaagtgg aaattttagc
      241 atgtgaagca tggagtgaat taggacatat accgaaagct gacgtacaaa aaattcgtca
      301 aaacgcaaag gtaaatgtcg aacgtgcaca agaaattgaa caagaaacgc gccatgatgt
      361 tgtagccttt actagacaag tttctgaaac gctaggtgaa gaacgtaagt gggtacatta
      421 tggtttaact tctactgatg ttgtagatac agctttaagt ttcgttatta aacaagcaaa
      481 tgacattatt gaaaaagatt tagaaagatt tatcgacgtt ttagctgaaa aagcaaaaaa
      541 ttataaatat acattgatga tgggacgtac acatggagtg catgcagaac caactacatt
      601 tggtgtcaaa atggcattgt ggtacacgga aatgcaacgt aacttacaac gtttcaaaca
      661 agtaagagaa gaaattgaag tagggaaaat gagtggtgca gtaggtactt ttgctaacat
      721 tcctcctgaa atagaaagtt atgtgtgtaa acatttaggt attggcacag cgcctgtatc
      781 aacgcaaaca ttgcaacgcg atcgacatgc atactatatt gcgacattag cattaatcgc
      841 gacatcacta gaaaaatttg ccgttgaaat acgtaacctt caaaaaaccg aaacgagaga
      901 agttgaagaa gcatttgcaa aaggccaaaa aggttcatct gcaatgcctc ataaacgaaa
      961 tccaattggt tctgaaaata tcacaggtat ttcaagagtc attcgtggtt atattactac
     1021 agcttatgag aatgtgccat tatggcatga aagagatatt tctcattctt ctgctgagcg
     1081 tattatgtta ccagatgtta caatagcact agattatgca ttgaatcgtt tcacaaatat
     1141 tgtggaccgt ttaactgtat ttgaagataa tatgcgtaat aacatagata aaacatttgg
     1201 tctgattttc tcacaacgtg tcttattagc attaattaat aaaggtatgg ttcgtgaaga
     1261 agcatacgac aaagtacaac caaaagcgat gatatcttgg gaaacgaaaa caccgttccg
     1321 tgaattaatc gaacaagatg agtctattac aagtgtttta acaaaagaag aattggatga
     1381 atgtttcgat cctaaacatc atttgaatca agttgacact attttcgaac gcgctggatt
     1441 agcataaaat taaataaagt aacgattcag atttaataaa atataaatcg ctacacttta
     1501 atagattaat gcgttaaaat gacataaaga ataaaattat aggggttgtt tcgatatgca
     1561 aattgaaaaa ttacgaggtg cagcgttaga tgaattgttt gatgcaatac taacgttaga
     1621 aaatagagaa gaatgttacc aatttttcga tgatttgtgt actgtaaatg aaattcaatc
     1681 actgtctcaa agattacaag ttgctaaaat gattaagcaa ggttatacct atgcaacgat
     1741 tgaacaagaa tctggagcat cgactgcaac gatttctaga gtgaagcgtt cattacaatg
     1801 gggtaatgat gcttatacaa tgattttaga tcgtatgaat attgaaacaa atgaataata
     1861 aatgaacgat aaacaatggt tatctatctg cactaataag gtagataatc attgtttttt
     1921 cacgaaaaaa tttacagagt aaaagaactt aaatttcata ttaagtcttt agaactcgac
     1981 acttaaaaat gctataatca tatgtatgtt aaaaaaggag tttcggaaaa tgtatgacat
     2041 taaaaaatgg cgccatattt ttaaattaga cccagctaaa catatttcag atgatgattt
     2101 agatgcgatt tgtatgtctc aaacagatgc aattatgatt ggtggaactg atgacgttac
     2161 tgaagataat gtcattcatt taatgagcag agtaagaaga tacccattgc ctttagtact
     2221 tgaaatatca aacatcgaaa gtgtaatgcc tggttttgat ttttattttg tacctacagt
     2281 actgaacagt acagatgttg tatttcacaa tggtacatta ttagaagcgc ttaaaacata
     2341 tggacatagt atagattttg aggaagtaat atttgaaggg tatgtcgtgt gcaatgctga
     2401 tagcaaaatg gcaaaacata ccaaagcaaa tacagattta acaacagaag atttagaagc
     2461 atatgcccaa atggtcaatc atatgtatcg attaccggtt atgtatatag agtatagtgg
     2521 catttatggc gacgtatcaa aggttcaagc tgtctcagaa catctaacag aaacgcaact
     2581 tttttatggt ggcggtattt cctcagaaca acaagcgaca gagatggcag ctattgcaga
     2641 tacaattatc gtcggtgata ttatttataa agatattaaa aaagctttaa aaacagtaaa
     2701 aataaaggag tctagtaaat gaatgcgtta ttaaatcata tgaatacaga gcaaagtgaa
     2761 gctgtaaaga caacagaagg accattgtta attatggcag gtgctggttc agggaagaca
     2821 cgtgttttaa cacatagaat tgcttattta ttagacgaaa aagatgtctc accatacaat
     2881 gttttggcta ttacttttac aaataaagct gcaagagaaa tgaaagaacg tgttcaaaaa
     2941 ttagtaggtg atcaagcaga agttatttgg atgtcaacat tccactcaat gtgtgttcgt
     3001 attttacgtc gtgatgcaga tcgaattggt atagaacgca attttacgat aattgatcct
     3061 acagaccaaa aatctgttat taaagacgtc ttaaaaaatg aaaatattga tagtaaaaag
     3121 tttgaacctc gtatgtttat cggtgcgatc agtaatttga aaaatgaact taaaacacct
     3181 gcagatgctc aaaaagaagc cacagattat cactcgcaaa tggtagcaac ggtttatagt
     3241 ggatatcaac gccaattgtc acgtaatgaa gcgttagatt ttgatgacct tattatgaca
     3301 acgattaact tatttgagcg tgtaccagaa gttctagaat attatcagaa caaattccaa
     3361 tatattcatg tagatgagta tcaagatact aataaagcac aatacacatt agttaaatta
     3421 ttagcaagta agtttaaaaa cttatgtgtt gtaggtgact cagatcagtc aatttatggt
     3481 tggcgtggtg ctgatattca aaatatctta tcatttgaaa aagactatcc agaagcgaat
     3541 acaatctttt tagagcaaaa ttatcgttcg acgaaaacga ttttaaatgc ggctaacgaa
     3601 gtgattaaaa ataattctga acgtaagcca aaaggactgt ggactgcaaa tacgaatggt
     3661 gagaaaattc attactatga agcaatgacg gaacgtgatg aagcggaatt tgtaatacga
     3721 gaaattatga agcatcaacg taatggtaag aaatatcaag atatggcaat tttatataga
     3781 acgaatgcac aatcacgtgt acttgaggaa acattcatga aatctaatat gccatacaca
     3841 atggttggtg gccaaaagtt ctatgaccgt aaagaaatca aagatttatt aagttatttg
     3901 cgtataattg ccaatagtaa tgatgacatt agtttgcaac gtattattaa tgttccaaaa
     3961 agaggtgtag gtccttcatc tgttgaaaaa gttcaaaact atgcacttca aaacaatatc
     4021 agtatgtttg atgcacttgg agaagctgat tttatcggat tgtcaaaaaa ggtgacacaa
     4081 gagtgtctta atttttacga gttaatccaa agcttgataa aagaacaaga atttttagaa
     4141 attcatgaaa tagttgatga agtattacaa aagtctggct atcgagaaat gcttgaacgt
     4201 gaaaatacat tagaatctcg aagtagatta gaaaacatcg atgaatttat gtcagtacca
     4261 aaagactatg aggaaaatac cccattagaa gaacagtcat taattaactt tttaacggat
     4321 ttatcgttag tagctgatat tgatgaggca gatactgaaa atggcgtaac actaatgacg
     4381 atgcactcgg ctaagggcct tgaatttcca attgtcttta taatggggat ggaagaatct
     4441 ttattcccac atattagagc gattaagagt gaagatgatc atgaaatgca agaagaacgt
     4501 cgtatttgtt atgtagcaat tacaagggct gaagaggtgt tatatatcac tcatgcgaca
     4561 tcaagaatgt tatttggtcg ccctcagtca aatatgccat ccagattttt aaaggaaatt
     4621 ccagaatcac tattagaaaa tcattcaagt ggcaaacgac aaacgataca acctaaggca
     4681 aaaccttttg ctaaacgcgg atttagtcaa cgaacaacgt caacgaaaaa acaagtattg
     4741 tcatctgatt ggaatgtagg tgacaaagtg atgcataaag cctggggaga aggcatggtg
     4801 agtaatgtaa acgagaaaaa tggctcaatc gaactagata ttatctttaa atcacaaggg
     4861 ccaaaacgtt tgttagcgca atttgcacca attgaaaaaa aggaggatta agggatggct
     4921 gatttatcgt ctcgtgtgaa cgagttacat gatttattaa atcaatacag ttatgaatac
     4981 tatgtagagg ataatccatc tgtaccagat agtgaatatg acaaattact tcatgaactg
     5041 attaaaatag aagaggagca tcctgagtat aagactgtag attctccaac agttagagtt
     5101 ggcggtgaag cccaagcctc tttcaataaa gtcaaccatg acacgccaat gttaagttta
     5161 gggaatgcat ttaatgagga tgatttgaga aaattcgacc aacgcatacg tgaacaaatt
     5221 ggcaacgttg aatatatgtg cgaattaaaa attgatggct tagcagtatc attgaaatat
     5281 gttgatggat acttcgttca aggtttaaca cgtggtgatg gaacaacagg tgaagatatt
     5341 accgaaaatt taaaaacaat tcatgcgata cctttgaaaa tgaaagaacc attaaatgta
     5401 gaagttcgtg gtgaagcata tatgccgaga cgttcatttt tacgattaaa tgaagaaaaa
     5461 gaaaaaaatg atgagcagtt atttgcaaat ccaagaaacg ctgctgcggg atcattaaga
     5521 cagttagatt ctaaattaac ggcaaaacga aagctaagcg tatttatata tagtgtcaat
     5581 gatttcactg atttcaatgc gcgttcgcaa agtgaagcat tagatgagtt agataaatta
     5641 ggttttacaa cgaataaaaa tagagcgcgt gtaaataata tcgatggtgt tttagagtat
     5701 attgaaaaat ggacaagcca aagagagtca ttaccttatg atattgatgg gattgttatt
     5761 aaggttaatg atttagatca acaggatgag atgggattca cacaaaaatc tcctagatgg
     5821 gccattgctt ataaatttcc agctgaggaa gtagtaacta aattattaga tattgaatta
     5881 agtattggac gaacaggtgt agtcacacct actgctattt tagaaccagt aaaagtagct
     5941 ggtacaactg tatcaagagc atctttgcac aatgaggatt taattcatga cagagatatt
     6001 cgaattggtg atagtgttgt agtgaaaaaa gcaggtgaca tcatacctga agttgtacgt
     6061 agtattccag aacgtagacc tgaggatgct gtcacatatc atatgccaac ccattgtcca
     6121 agttgtggac atgaattagt acgtattgaa ggcgaagtag cacttcgttg cattaatcca
     6181 aaatgccaag cacaacttgt tgaaggattg attcactttg tatcaagaca agccatgaat
     6241 attgatggtt taggcactaa aattattcaa cagctttatc aaagcgaatt aattaaagat
     6301 gttgctgata ttttctattt aacagaagaa gatttattac ctttagacag aatggggcag
     6361 aaaaaagttg ataatttatt agctgccatt caacaagcta aggacaactc tttagaaaat
     6421 ttattatttg gtctaggtat taggcattta ggtgttaaag cgagccaagt gttagcagaa
     6481 aaatatgaaa cgatagatcg attactaacg gtaactgaag cggaattagt agaaattcat
     6541 gatataggtg ataaagtagc acaatctgta gttacttatt tagaaaatga agatattcgt
     6601 gctttaattc aaaaattaaa agataaacat gttaatatga tttataaagg tatcaaaaca
     6661 tcagatattg aaggacatcc tgaatttagt ggtaaaacga tagtactgac tggtaagcta
     6721 catcaaatga cacgcaatga agcatctaaa tggcttgcat cacaaggtgc taaagttaca
     6781 agtagcgtta ctaaaaatac agatgtcgtt attgctggtg aagatgcagg ttcaaaatta
     6841 acaaaagcac aaagtttagg tattgaaatt tggacagagc aacaatttgt agataagcaa
     6901 aatgaattaa atagttagag gggtatgtcg atgaagcgta cattagtatt attgattaca
     6961 gctatcttta tactcgctgc ttgtggtaac cataaggatg accaggctgg aaaagataat
     7021 caaaaacata acaatagttc aaatcaagta aaagaaattg caacggataa aaatgtacaa
     7081 ggtgataact atcgtacatt gttaccattt aaagaaagcc aggcaagagg acttttacaa
     7141 gataacatgg caaatagtta taatggcggc gactttgaag atggtttatt gaacttaagt
     7201 aaagaagtat ttccaacaga taaatatttg tatcaagatg gtcaattttt ggacaagaaa
     7261 acaattaatg cctatttaaa tcctaagtat acaaaacgtg aaatcgataa aatgtctgaa
     7321 aaagataaaa aagacaagaa agcgaatgaa aatttaggac ttaatccatc acacgaaggt
     7381 gaaacagatc ctgaaaagat tgcagaaaaa tcaccagcct atttatctaa cattttagag
     7441 caagattttt atggtggtgg agatacaaaa ggtaagaata ttaaaggtat gacgattggt
     7501 ttagctatga atagtgttta ttactataaa aaagaaaaag atggatcgac ttttagtaaa
     7561 aaactagatg atagcgaagt taaaaagcaa ggtaaacaaa tggctagtga gatattatca
     7621 aggttacgtg aaaatgatga tttaaaagat ataccaattc attttgcaat ttataagcaa
     7681 tcaagtgaag attcaatcac accaggtgaa tttatcactc aagcgactgc agaaaagagt
     7741 caaacaaagc ttaatgaatg gcataatatc aatgaaaaat cagctttatt accttcttca
     7801 acagcagcag attatgatga aaatttaaat aataatttca agcaatttaa tgataatttg
     7861 caatcatatt tttctaattt cacacaagca gtagggaaag ttaaatttgt tgataaaaag
     7921 ccacaacgat tagtagtaga tttaccaatc gattactatg gacaagctga aacaattggt
     7981 attacacagt acgttactga acaagcgaat aaatatttcg ataaaatcga taactatgaa
     8041 attcgtatta aagatggtaa ccaaccacgt gctttaatta gtaagacaaa agatgacaaa
     8101 gaaccgcaag ttcatattta cagtaattaa ttgtaaatta aagataataa aaaggtacca
     8161 atgttagtca taattttgat taacattagt acctttttaa tttgttaaga cttatgaatt
     8221 atttttctct aacgatgtca cgaactttgt ttaagtcagt ttcaacaaat gcaccaggtt
     8281 ttttagtaag tttacttaca acatatgtaa cgattacact tacaataaat ccaggaataa
     8341 tttcatataa gccgaatatt tcgttgatat gtgccaatgg tttaatccac gcaatccaaa
     8401 cgataacgac taaggcacct gaaaccattc cacttacagc accggcacgt gtcaaacctt
     8461 tccagtaaag tgcaaatagc acaagtggac tgaacgatgc accaaatccg gcccaagcgt
     8521 tacctactaa gtttagaatt gtgtcgtttg gattccatgc aatcgcgatg gcaacaattg
     8581 ctacaactaa tacagataat cttccaatca taacaaattc tttttggtgc gttttagctt
     8641 tttcttcacc acgaattaat ttataaaagt cttccgttag tgaactagat gttacaagta
     8701 attgtgaaga aatcgtgctc ataattgcag ctagaatcgc agcaagtaag aaaccaccta
     8761 caagaggatg gaagagtact tgactcatca cgatgaataa tgtctcagga tcttctagtt
     8821 taatatgata agcaggtacg aatgcaatac ctgttaaacc aacagccaca gcgcctaata
     8881 aaccaacagc catccagcta atacctaaac gtctagcttt aggtagcatc ttgtgtgatt
     8941 taatagacat aaaccttata atgatatgag gttgaccgaa ataacctaat ccccatgaaa
     9001 atagagagat aattcctata aatgataacc ctttaaataa atttaaattt gtaggtttca
     9061 tagctgctac atcatgaaac gttccccagc cgtttaaatt catcatagca acaataggga
     9121 ccataaccat cgcaattaac ataatgacac cttggaagaa atctgtaatt gatacagcta
     9181 aatatccacc aaagaaagta tagaaaatga caatgaaagc aactaatatt aaaccgaaat
     9241 gataatctaa tccaaaagca ctttcaaata gtttaccacc agatacgaaa ccagaatgtg
     9301 tatataatgt aaagaatact acgataatca atccagaaat aatctttaac acattatttt
     9361 tatcgtttaa acgattttta aagaaatctg gtaatgtaat tgcatctcca gctaattcgg
     9421 tataaacacg aagtctagga gcaacaacaa agtaatttat ataagcacct aatgttaaac
     9481 cgattgtaat ccacatagct gatagaccag tgctatagac agaaccaggt agccccataa
     9541 tcatccatcc actcatatct gaagctccag ctgataatgc agtaatatac ggtccaatac
     9601 tacgtccacc taacatgtac tcgcttaggt taccagttgc ttgcttgtaa ccgtaaaagc
     9661 caataacgat tagtatcaag aagtagacgg caatcataat ataagtttgc caattggcat
     9721 ctacttgttg acttaatgct gtccccattg taagcatgat aaacactccc tttatttaat
     9781 ttataattta gttacttgaa aacattatac ataaagataa atgtttagaa aagtaggaaa
     9841 at
//
LOCUS       DALAGF010000099         9792 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.099, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000099 DALAGF010000000
VERSION     DALAGF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9792
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.099"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(59..967)
                     /locus_tag="O2692_002211"
                     /pseudo
     CDS             complement(59..967)
                     /locus_tag="O2692_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            complement(1192..1404)
                     /locus_tag="O2692_002212"
                     /pseudo
     CDS             complement(1192..1404)
                     /locus_tag="O2692_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc peptidase"
     gene            complement(1479..3014)
                     /locus_tag="O2692_002213"
     CDS             complement(1479..3014)
                     /locus_tag="O2692_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCZ4299826.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            complement(3065..4609)
                     /locus_tag="O2692_002214"
     CDS             complement(3065..4609)
                     /locus_tag="O2692_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCZ4299827.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVKYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSL
                     KASSLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNEKVTFDIPQNVSVNEPFEMTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNVTIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            4838..5794
                     /locus_tag="O2692_002215"
     CDS             4838..5794
                     /locus_tag="O2692_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCZ4299828.1"
                     /translation="MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            6008..6781
                     /gene="phnC"
                     /locus_tag="O2692_002216"
     CDS             6008..6781
                     /gene="phnC"
                     /locus_tag="O2692_002216"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCZ4299829.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            6783..7583
                     /gene="phnE"
                     /locus_tag="O2692_002217"
     CDS             6783..7583
                     /gene="phnE"
                     /locus_tag="O2692_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCZ4299830.1"
                     /translation="MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            7580..8395
                     /gene="phnE"
                     /locus_tag="O2692_002218"
     CDS             7580..8395
                     /gene="phnE"
                     /locus_tag="O2692_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCZ4299831.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(8526..9704)
                     /gene="deoB"
                     /locus_tag="O2692_002219"
     CDS             complement(8526..9704)
                     /gene="deoB"
                     /locus_tag="O2692_002219"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCZ4299832.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
BASE COUNT         3168 a         1779 c         1543 g         3302 t
ORIGIN      
        1 aaaaatactt attttaagta gatgagcata agtgaacata gttctttagt tataataatt
       61 aattcaacca aaagtcgatt tgtttttgca attggttttc atttcctctt aaagatattt
      121 tcattaaatc tgtcaaatca atagacgcta tatttttcaa cttatctcta tatttatttt
      181 tagtacgtct ttctaaattt ccccattcct cttcttcgtg agttaataaa tgaagcattg
      241 ctcgttcttg tatattttca atcattttta aattcggttt taaaatatgc aaatcatcaa
      301 aacaatcttt ccaacaatca accatatctc gttttaattc aatttccaca cgccatagaa
      361 atgttgaatc aatttcaaca tctgcattat ctttacgttc ttgtttttat tataaatccg
      421 aataaaccta tcactattac gcacaccaaa atattttgtt tctggtttta cattacgtcc
      481 ataaaatata gttttcttta ccgacttatc tgacaatgca taatagtcat ttaaatcaaa
      541 ttcaaaatca aaagccaaat ctaatctcgt aaaactaaca tcgtccaaat aactgatgat
      601 attttgtttt aaccaaagca cttcatcatg cgaaagctta ttaggattaa attcaacgcg
      661 catactacgt ctattccaaa gagttgcttt tattttgtca tattcaatat aaactttttc
      721 tttaagagct ttagctttaa agtttgtttg taaaatatcc caaagccgaa tttcaggatt
      781 agtactcata aaatgtgaaa gtctctctgc gttagacatg ctaagattcc caacaatcgt
      841 tatagcgtca aaagacaatt ttggaatagc tagtgacatc ctatgtcgat ttaaccggct
      901 attaccggat attagagtat ccagttttac aaatggatga aacgaaattc aaaacactaa
      961 aaaatatgtt ccactaacag caaaaaaata ccattatgtt cctactaaaa aactaaaaat
     1021 actggagaac aaatgtcagg atataactta ggatactatg taataaaaat ttacaataaa
     1081 aaaacaggaa aacaaatttc aagtaaaaga atacccatac aaagaggata aaataaaaaa
     1141 cctcgaactg aaatgatgat cttttcagct cgaggtttaa atattggtgc cttatttata
     1201 tagattcgtt atattatatt ctctattttc attaacataa tccttaaaga gttttaaatt
     1261 aatacctgct agatgattca aaaatgtttc atcaactttt aaataattca ataatttttg
     1321 tggtgtcagt aaatttctat caaaatacaa ctttaataaa ctattcattt tgacaggacg
     1381 tgacatttca atcacgtcgt ctaaagataa tactttctcg ctttaaacaa aaacaaaaac
     1441 ttacccgatt aaaatcaagt aagttttaca tatatccgtc acttttcaac tttaaaatca
     1501 acaacttgcg ggatgcgcat caaattatta tttgataaaa aatcaataag taattgtgcg
     1561 ccttcaactt gaatatcttt tacaactggc gcgtcgatat acatatcata ctgaccaccg
     1621 cctactgcac gataattatt tacacaaatt gtatatgtct gctttaaatc aactgcgtga
     1681 ccttgaatca tcatattgct cacacgttgt ccctttggtc ttccaacatg aatggtataa
     1741 cttacgccac catatatatc ataattaaag tgttgtggtt tgggttcaag gaagtctgcg
     1801 ctcacactaa cttcatcatt tttcacgtca aaatattctg ctgatcgttc aatggcttct
     1861 ttaagtttgg caccacttac agctaaaact ttaaatgtat ttggaaatgg gtaattgtta
     1921 ataacatctc gcatcgtcac gacttgcttg aaaccactag cagaatcaaa caaagctgta
     1981 caggcaacat ctgcgtcact tttttctaat aaagcgtaat tcataaaatt tgtaaaagga
     2041 tgcggtgcca cacgtgcctc aaatgcatga ttaatcgtca tatcatatgg caatgtagta
     2101 atttcgtaat ctaaccagtc ctctaactgc tttcgtaaat gttggtcatc ttcatcaata
     2161 gtaaatgtgg aatcatctat aacaggaagt aattcacatg attcaacgga tagattttca
     2221 tattcatcag tactcaagac tactctgcct acagttgtac ctctcgtacc aggttgaatc
     2281 acagccgttt gcttaaacct ttcagcaatt tgtcgatgtt ggtgacccgt aataaagata
     2341 tctatatctt tagaaaacgc ttctaacatg gcatatcctt cattttcacc cgttaatact
     2401 tcggtcggcg taccactttc taaatccttt tcaaatccac catggtaaca aaccacaatg
     2461 atatctgcat gtcgcttcat ttcaggtaag tattgttgaa gtatttcaaa agcactatga
     2521 aacgtaagtg actgaatatg ctctggttgt tcccaatggg gaataaattg tgtcgttaaa
     2581 cctatcacac caacagtttg atctccaacc tgaaaatact tcacaccgtt atcagtcaat
     2641 gtactatcat tttcatatat attagcgcac aaaactggat aattgagtct gcgtaaagtg
     2701 tcttttaagt atggtaatcc ataattaaat tcatgattac caagcgtacc aaagtcgaat
     2761 gccattcgat tataaaaatc aactaaaggc tggctactgc cgctatgcgc gattaagtaa
     2821 ttacaaaatg gtgacccttg caaaaaatca ccattatcta ttttaaaact ttggtcatac
     2881 tgccttctgt cttgttctat aacatgattc gctagtaaca atcccatagg ttgatattga
     2941 tttctactcg taaaatctgt tgggaaaata taaccatgta cgtcactcac gacataaaat
     3001 gctatgtttg acatcctcac tcactccttc aatcacaaac atctttctta tttctattat
     3061 atatttattt gaagtctgtt gtaatcaagg ttttgtcacc gagttttaaa cgaatctttg
     3121 aaccttccat actttcaagt actttagcat tgaccttaat tgtgacattt ccgttttcat
     3181 ctgctttaac tgttggcaaa gtactgtaac ctggtgggtt ataatcgtta tctttacttg
     3241 aaaattgtcc gatttgacgt ccgccttctt tgtaaatacc aactctaaga ttttcaagtg
     3301 tttgatttgc ttcaaatcct tttaaatgta ttgtcatttc aaatggctca tttacagaaa
     3361 cattttgcgg gatatcaaat gttacttttt cgttctgatt tggtggtgta tgatcatctg
     3421 gtgtgtttgg ctgaggatct gcgccttttt cgctgccata actacctgct ttaaatgttg
     3481 ttggatcata ccatttataa ccactcggcg gttgtgacca tggctctttt tcaggctcag
     3541 ttgaacgctc tggtcgttca aaatcaagca acttagtctt tgtatctaat gttaggctac
     3601 tcgccttaag tgatttccca tcattatctt tagacatcca agccgttata ttatttaata
     3661 gcttaccgtt gtcttgttct ttaaaaccat catatgtttt cttcttttct ccattatctt
     3721 ctcttacata tttgggcgaa ctatcttcca caagtgatga atcaccgata aatgctgctt
     3781 taccttttcc aactttagaa attgctacat aggggccttc tgctttaccg cccccattat
     3841 aaataccttg atctacagca tgtgaccatt tacttttcgc tggcaattgt tctggtgtat
     3901 acacaatacc ttttgctttc tctggattag taattgctaa tgtcgatccg gcatgcatag
     3961 agacagattt cacaccttca gtaataccga aactttcttt tgaagaaaca atattgctcg
     4021 tatttaaatc acctagtgca ttatatcgaa aacgtacgcc aaagtttgta gataaccaat
     4081 ctgaactttt cacaccttgc attgcagtag aacttttttc ttctgcattc atacctttcg
     4141 acatatcttc atatgctcca cgtcgataac cattcattgc ctccgatgaa tcaatacgat
     4201 ttaaatttcg gtcagcattg taatgatctg aaataaagac aacattgcca ccttgtttca
     4261 catatttaac aattgctgcc tgttctgatt ctttgaaagg aatgttagcc tcaggaatta
     4321 caaatatttt ggaacttttc aaacttgctt ctgttatgtt cgaatgacca tcaatagctt
     4381 taacgtcata accttgtttt tgtattgaat ccgcataatc tgaaaatgca ccatcactaa
     4441 cccaatctgc agcaccagct gtttgaccat gagaacgatc gaataatacc gttcgctgtt
     4501 gctttgtagg ttgcgattca tgcgttatag ctaaagattg cggtaaagca cttaatgata
     4561 ccgttgcaac aattgcagag acagttaatg acttatatat ttttttcatt ttgtgaggct
     4621 ccttttaaaa taaatttgtt cttgaattat aggataaaaa ttcgttgcat atgagcaatt
     4681 taacgaaaaa tttacaaaat cttatcaaac tcttaaagaa agttattaaa attcattttt
     4741 ataaaatact ttttaacatt taaatgtggt acgctataag tgtaatttca ttgcatacat
     4801 attacacgat taagaatgtg aaggggacag ttatcaaatg aaaaatttta agtgtttatt
     4861 tgtattaatg ttagcagtca ttgtttttgc agcagcatgt ggaaactcaa gttctttaga
     4921 taatcaaaag aacgctagta atgattcgga ttctaaatca ggaggataca aacctaaaga
     4981 attaaccgtt caatttgtac cttcgcaaaa tgctggaaca ttagaagcta aagcaaaacc
     5041 attagaaaaa ttactatcta aagaattagg gattccagtt aaagtgtctg tatcaactaa
     5101 ctacaataca attgttgaag ctatgaagtc taaaaaagtt gatgttggtt tcttaccacc
     5161 aacggcatac acattagcac atgatcaaaa agcagctgat ttattattac aagcacaacg
     5221 tttcggtgta aaagaagatg gttcagcaag taaagaactt gtagatagtt ataaatcaga
     5281 aattcttgtt aaaaaagact caaaaattaa aagcttgaaa gatttaaaag gtaagaaaat
     5341 tgccttacaa gatgtaacat caactgctgg atatacattc ccacttgcga tgttaaaaaa
     5401 cgaagcaggt attaatgcaa ctaaagatat gaaaattgtg aatgttaaag gtcatgacca
     5461 agcagttatc tcattattaa atggagatgt agatgctgcg gctgtattta acgatgcacg
     5521 taatactgtg aaaaaagacc aaccaaatgt atttaaagac acacgaattt taaaattaac
     5581 acaagctatt ccgaatgaca caatttctgt aagaccagat atggataaag attttcaaga
     5641 aaaattgaaa aaagctttta tagacattgc taaatcaaaa gaaggtcaca aaattattag
     5701 cgaagtttat tcacatgaag gatacacaga aacgaaagat tcaaatttcg acattgtaag
     5761 agagtacgaa aaattagtta aagatatgaa ataatcatta tttaacaaat gaatcattag
     5821 cgaatttggt attaaaagct ttcgttcaat agatatattc tagattaata ttgaaaagct
     5881 aggcgctaaa ctgaaacaga tatagaaagg tgtcgctgta catttgaaac catttgtaca
     5941 cagaaaccca atgtctatga tatttcagtt taccttggct tttctttatt aaagaaaggt
     6001 gtcaaacatg agtcaaatcg aatttaaaaa cgtcagtaaa gtctatccta acggtcatgt
     6061 aggcttgaaa aatattaact taaatattga aaaaggtgaa tttgcagtta ttgtcggact
     6121 atctggtgct gggaaatcca cgttattaag atctgtaaat cgtttgcatg atatcacgtc
     6181 aggtgaaatt ttcatccaag gtaaatcaat cactaaagcc catggtaaag cattattaga
     6241 aatgcgccga aatataggta tgattttcca acattttaat ttagttaaac ggtcaagtgt
     6301 attacgaaat gtactaagtg gacgtgtagg ttatcaccct acttggaaaa tggtattagg
     6361 tttattccca aaagaagaca aaattaaggc aatggatgca ctagaacgcg tcaatatctt
     6421 agataaatat aatcaacgct ctgatgaatt atcaggtggc caacaacaac gtatatctat
     6481 tgcacgtgcg ctatgccaag aatctgaaat tattcttgca gatgaaccag ttgcttcatt
     6541 agacccatta actacgaaac aggttatgga tgatttaaga aaaatcaacc aagaattagg
     6601 catcacaatt ttaattaatt tacattttgt tgacttggca aaagaatatg gcacacgcat
     6661 cattggttta cgtgatggtg aagttgtcta tgatggtcct gcatctgaag caacagatga
     6721 cgtatttagt gaaatatatg gacgtacaat taaagaagat gaaaagctag gagtgaacta
     6781 acatgccttt agaaatacct acaaagtatg actccctttt aaagaaaaag gtttctttaa
     6841 aaacgagttt taccttcatg ttaatcattg tgcttatcat ttggagcttc atttatacgg
     6901 gatttaattt tggagattta atgataggaa tacctcaaat aggtgatcta ttcaaacaaa
     6961 tgattccacc tgatttcgag tatttacaac aaattacaac gccaatgtta gataccattc
     7021 gaatggctat cgtaagtaca gtattaggta gcatcgtttc aataccaatt gcgttattat
     7081 gtgctagcaa tatcgttcat caaaagtgga tttcaatacc ctcgcgcttt attttaaata
     7141 tagttcgtac tattccagat ttgttattag cagcaatctt tgtggctgta tttggaatcg
     7201 gtcaaattcc agggatatta gcactgttta ttttaactat ctgtattatt ggaaaattat
     7261 tatatgaatc attggaaacg atagatccag gtccaatgga agcaatgacg gctgttggcg
     7321 ctaataaaat aaaatggatt gttttcggtg ttgtaccaca agccatatcg tcatttatgt
     7381 catacgtatt atatgcattt gaagtaaata tacgtgcttc agctgtgctt ggattagtcg
     7441 gcgctggcgg tattggattg ttttatgatc aaacacttgg tttatttcaa tatccaaaaa
     7501 cagcaacgat tattttattt actttagtta tcgtcgtcgt cattgattac atcagtacga
     7561 aagtgagggc acatctcgca tgacacagga aatagcaaaa tataatgttc acacaaaagc
     7621 acacaaacga aaattgatta aaagatggct tattgcaatt gtcgtcttag ctattatcat
     7681 ctgggcattt gcaggtgtac caagtttaga acttaaaagt aaatcattag aaatcttaaa
     7741 atccatattc agcggattat tccatcctga tatcagctat atctatatac cagatggcga
     7801 agacttatta cgtggtttac ttgaaacctt tgcgatagcc gttgtaggta ctttcatcgc
     7861 cgcaattatc tgtattccat tagcatttct aggtgcaaat aatatggtaa agctacgccc
     7921 agtttcaggt gttagcaaat ttattttaag tgttatacgt gtcttcccag aaattgtaat
     7981 ggcacttata tttatcaaag ctgttggccc aggttcattt tcaggtgtat tagctttagg
     8041 tatccattcc gtaggtatgc ttgggaaact tttagctgaa gatattgaag gtctagattt
     8101 cagtgctgta gaatcattaa aggccagtgg tgcgaataag attaaaacac tcgtatttgc
     8161 agtcatacca caaattatgc ctgcctttct atcactcata ctttatcgct ttgaactaaa
     8221 cttacgttca gcttctatac tggggctaat tggggctggt ggtatcggga caccactcat
     8281 atttgccatt caaacacgtt cttgggaccg tgtaggtatt atattaatcg gtttagtact
     8341 aatggtcgca attgtcgatt taatttccgg ttcaatccga aaacgtattg tttaacatta
     8401 aatcaggata ctcctaaata agaagtccta ccgtcttacg tttctctatt ataataaaaa
     8461 cagcagtgaa gaaaactatt gttatagtta acttcactgc tgtttttata atatctaaat
     8521 ttattctatt tcaattcctt taaataactt ttaccgaact ctggtaatgt tacgttgaaa
     8581 ttatctgcta tagttgcacc gatagaactg aatgtagtat cactttctag tgcatgacca
     8641 cctttaaatt tcggactgta cataattact gggatatatt ctctcgtatg gtccgtacct
     8701 ggcgctgtcg ggtcattacc atggtctgct gtaataatta ctaaatcgtc ttcttttaag
     8761 ttgctaaaca gttctggcaa gcgatcatcg aaatctttaa ttgcttgtgc ataacctggt
     8821 ttatcacgac gatgaccgta taatgcatca aagtctacta agtttaagaa gctaatacct
     8881 gtgaaatctt tcttaacaat tttcatcaat tgatccatac cgtccatgtt actcttcgta
     8941 cgaaccgctt ctgttacacc ttcaccatca taaatgtcat taattttacc gatggcaata
     9001 acatcataac caccgtcttt caaatgatct aagacagttt taccaaaagg ttttaacgca
     9061 tagtcatgtc gattagatgt acgtgtaaag tttcctggtt caccaacata tggacgtgcg
     9121 ataatacgac caattaaata tttagggtct tttgtcaact cacgaacctt ttcacaaata
     9181 tcatataact cttctaatgg gataatgtct tcatgtgcag caatttgcaa tactgggtct
     9241 gcacttgtat aaacaattaa gtcaccagtt ttcatttggt gctcgcccca ctcatcgata
     9301 atttgcgtac ccgatgccgg tttgttagca acaactttac gacctgtcat ttcttcaatt
     9361 tgttgaatta actcttcagg gaatccatta gggtatactt taaaaggttg cataatattt
     9421 aatcccataa tttcccagtg accagtcatt gtatctttac caactgaagc ttcactcaat
     9481 ttagtatagt atgcttctgg ttgttcaact gcatttacta ctggtaattt atcgatgttc
     9541 cctagaccta acttttcaag gtttggtaaa gtttgatcga aaccttctaa ggtatgtctt
     9601 aaagtatgtg aaccttcatc tttaaaatca gctgcgtctg gcgcttcacc aatacctact
     9661 gaatccatta cgattaaatg tacacgatta aatggtcttg tcatagctat cactcccaaa
     9721 atttatatat attagtaatc tgaatctgct tctaaacctt gcataatttg aacacctgcg
     9781 ctcgcaccaa ta
//
LOCUS       DALAGF010000100         9751 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.100, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000100 DALAGF010000000
VERSION     DALAGF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9751
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.100"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..338)
                     /gene="deoC"
                     /locus_tag="O2692_002220"
     CDS             complement(<1..338)
                     /gene="deoC"
                     /locus_tag="O2692_002220"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCZ4299833.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYHFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVV"
     gene            653..1363
                     /gene="deoD"
                     /locus_tag="O2692_002221"
     CDS             653..1363
                     /gene="deoD"
                     /locus_tag="O2692_002221"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCZ4299834.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(1484..1927)
                     /locus_tag="O2692_002222"
     CDS             complement(1484..1927)
                     /locus_tag="O2692_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCZ4299835.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(2127..2540)
                     /locus_tag="O2692_002223"
     CDS             complement(2127..2540)
                     /locus_tag="O2692_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HCZ4299836.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
     gene            complement(2998..4371)
                     /locus_tag="O2692_002224"
     CDS             complement(2998..4371)
                     /locus_tag="O2692_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HCZ4299837.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRISNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
     gene            complement(5043..5273)
                     /locus_tag="O2692_002225"
     CDS             complement(5043..5273)
                     /locus_tag="O2692_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299838.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            complement(5304..6242)
                     /locus_tag="O2692_002226"
     CDS             complement(5304..6242)
                     /locus_tag="O2692_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HCZ4299839.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            complement(6677..7342)
                     /locus_tag="O2692_002227"
     CDS             complement(6677..7342)
                     /locus_tag="O2692_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCZ4299840.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGDQDIKDALTQFEN
                     ETD"
     gene            7494..7814
                     /gene="czrA"
                     /locus_tag="O2692_002228"
     CDS             7494..7814
                     /gene="czrA"
                     /locus_tag="O2692_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HCZ4299841.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            7816..8796
                     /gene="czrB"
                     /locus_tag="O2692_002229"
     CDS             7816..8796
                     /gene="czrB"
                     /locus_tag="O2692_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HCZ4299842.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
     gene            9062..>9751
                     /locus_tag="O2692_002230"
     CDS             9062..>9751
                     /locus_tag="O2692_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ4299843.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     AQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSTAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKF"
BASE COUNT         3106 a         1737 c         1358 g         3550 t
ORIGIN      
        1 accactgctt caatgtcttg ttgtacatca tcaaaacgtc catcttttaa tgcgccgatg
       61 ttgatgacca tgtcaatttc atctgcacca ttttgaatcg catcttctgt ttcaaatgct
      121 ttcgtcgcag ttgtcgatgc acctaatggg aatcctatta ccgtacaaac taacacctct
      181 gaatcagcta gtcgctctgc tgcatattta acatgcgttg gattcacaca tacagattta
      241 aaatggtatg ctttcgcttc atcgatgatt tgatcgattt gcgtacgtgt tgactcaggc
      301 ttcaataaag tgtgatcaat caattttgca ctattcattt tctatctcct cctttatggt
      361 tgattataaa aatacggttg taaattagtt gattgagcgt caggttcatt taaatatcag
      421 gttagatgtt cgctttttat gtaaccgcat acatatacta ttacattaat tcatttccca
      481 taaacaaaca atacaattga acgtgatatc ttcattatga acgatgactt gacaacaagc
      541 taatcaggat tatattttta taattcttta attctatagt acaaaaattc gcaaaaaagg
      601 gaaacaaatg ttatcttaaa attattaatg aatattaagg agaagataac aaatgacaaa
      661 aggtacacca catattcaac caaatggagt aaaaattgct aaaacagtat taatgcctgg
      721 cgatccgcta cgtgcaaaat atattgctga taatttttta gaaaatgttg aacaatttaa
      781 cgatgtacgt aacatgtttg gttacactgg tacatataaa ggtaaagaag tttctgtaat
      841 gggttctggt atgggtattc caagtattgg tatttactca tatgagttat acaacttctt
      901 tgatgtagat acaatcattc gtatcggttc ttgtggcgca ttacaagaaa atgttaactt
      961 atacgatgtt attattgcac aagctgcatc aactaattca aattatgtag atcaatacaa
     1021 tattccaggt catttcgcgc ctatcgctga cttcgagtta gtaactaaag ctaaaaatgt
     1081 cgctgaccaa atcggtgcta ctacacacgt aggtaacgta ttatcttctg atacatttta
     1141 caatgccgat ccaacattca atgatgcttg gaaaaaaatg ggtattttag gtatcgaaat
     1201 ggaatcagct ggtttatatt taaatgcgat tcatgctggt aaaaaagcac ttggtatttt
     1261 cacagtaagt gatcatattt tacgtgacga agctactaca cctgaagaac gtcaaaattc
     1321 atttacacaa atgatggaaa tcgctttaga aatcgcagag taacttattt aaattgactt
     1381 taattgctct ttaacaatgc gattaaactc aaaaagccaa cacattctgg gcgtatcccc
     1441 atttatgtgt tggcttttat ttatattatt acttatctgt agattagctt aagtaagatt
     1501 taaacatcca attatgttta tctactgatg tttgcatacc tataaacata tcttctgata
     1561 catcatcgcc agcattacca gcaatttcga ttgcgttttc taattgtttt gagatatttg
     1621 tgaagtcttg tgataattct tcaaccattt gttctgcaga gtaacctttc gcagcttctt
     1681 taacaattga ttgctctaag cattcagtta atgtacctac agggtttcct cctaccgcta
     1741 aaattctttc agctaattcg tctacatatt ggcttgcttc attatataat tcttcaaatt
     1801 taacgtgtaa tgagaagaag ttaggccctt tcacatacca gtggaaattg tgtagctttg
     1861 tgtaagctac tgtccagttt gctacttgtt gattcaattc ttttacaaca tcttgttgat
     1921 tactcatatt taatacactc cttaaaattg tctacgtctt gcttgtttat attaattatt
     1981 atattataat aattctaatc tgtaaagtgt ttttgctctc ttttttataa ttattctaat
     2041 ctaaccgtaa agtgttccgc tatttaaatg cctaaatttt aattcatcat ctaatatatc
     2101 cattatcaat tagcaagtat gttttatcaa tcagtaagtt ttactttcgc attatcatct
     2161 aaaaagaacg atttttcata atcatttggt tggtgccatg ttgttttcca cattctattt
     2221 ctattattag caaacattcg ataaccgaaa ttacggactg gtttaggtac aagccataaa
     2281 cctacgccca ataatttagt tcgattaggt agtgctgtga ttagtttaat aatcgcctgc
     2341 gattcaaaat atatacgatc acctttctgt aaaatcacac tatttttatt tgccgcttct
     2401 ggatgttgtt taaaaaattg ttggccaact tcacctttta acgtggcaaa ttgataattt
     2461 cttggtaggt catgttgaat taaccaaatg acatagttat aacaatatat acagtttcca
     2521 tcataatata cgattggcat aatttattcg ctcctttaat agtacttata gtcatttttc
     2581 ctaatttaaa aacatttaaa caaggttgtc acttgataag tcctcgcgac acgaaattgc
     2641 cataaaattt atttttcagt tttatattta ttgtataatg tattttttat ttactttaaa
     2701 tttctcaatc tacagtgtaa tcttatttct tattattaac ttagcgtttg gtcattcgtc
     2761 ttattcaaca taccactgga aaactcatat aaagttttaa ttctgtaaca taaattcttt
     2821 tatattcata tactgtcttt attatttatt aaaataaaaa cgagtaatct atcataagct
     2881 cttaattagc tcttatggtt gattacccgt cattttttct ttatgcggtt attcaaatat
     2941 gcaattatgc aatttttaat cctatcctta cttcttttca aaaactacca tatatcatta
     3001 gtcatgtttg actttgacta tacgtgtgta ttttaaaaac tgctctgaat atttctcctt
     3061 aatttcctct aaactgtcat atgtaatacc tacaatatgc caattaggat tgaaataata
     3121 gaaagaatct ttttgtcgtg accatttaat catattaccg tctttattat aacgtggtaa
     3181 tgttacttca tagccttcta acacattaat atacgtttgg aaaatgtctg tatctatacg
     3241 attaaaatta tcaataacta aataactttt atccttttta ggcattaatt gagtaataaa
     3301 cccttcacga tagtataatg cccctgtttc atttggtaaa tacttgccat aaagcatatc
     3361 ttctgaaaaa tcaggatgac ctgttgttat aacaggattt gcattcgctt tttgcaataa
     3421 gtcattagcc gcttttaagc cttcttcttt tcgatctaca acaagtaaaa taaatggctt
     3481 taacgcttct tctttttcac tttcaagcat atctggtttt tgaaccattt caaatggaga
     3541 tttcaatcca ttattatcgc tcatttcaat aattgcatca tactgtgctt gtgacatact
     3601 tgcaatagcc tgttttgcat tttcttgaag gaaatataag tttttcaatt taggatgctt
     3661 atttaatgta cttaatgtaa tcggtgtaat gtctttctca taagacactt caatcactgt
     3721 actatttgtt ctaccaggaa ttggtggttt ttcatgaata tgctttgata cttctccaat
     3781 tccaacgaca gattgatttt tcgttcgatt ataaaaaata atattgtcgc cttcttctaa
     3841 ctgagtataa aaatgataac cattacgttt aataccgttg tacgtgtgcg tataaatcgt
     3901 atattggttt ccaggttcaa attcttcagt ttcagctaaa aagaaataac gcggtatctt
     3961 aatttcgcct ttaccaagac cacttattaa atcaaactct tctgcagtga tttgattgaa
     4021 caatgtctct ttcatattac ttatacgaaa ttccaaagct tcactacgct ttaaataatc
     4081 tgctgttaat ggtttcaatt gttcattaaa acgaaactgt acacgtattt tattttgtgc
     4141 acctgtttca acactaataa tttcaccaca tccaagtagt ccagtatccg tctgaacttg
     4201 ataaaagatg acttgatctc ctactttagc ctttttaaac gctctaaatc cttgagatgg
     4261 gttaaaatgt gcgcctgatt caaataaagc tgtttgtcct actaacggtt cattatgatt
     4321 ccaacggtta tatccacaat tcaaccaaaa ataattcgtt tctgctgtca tcttaatact
     4381 ccttaacctg aataaatttt agaaacacta tgaattacat tcttttagtg tttcttatgc
     4441 agttggacgc gtatgcgaac aactgtatac cctttgttca ctgcgatttt aatcgcattt
     4501 cctataacat tgtagcgccc aggacattaa tttacgtccc agacccttat cgttttcact
     4561 tctaagtaag tcgaactatt ttgctttaca acaagtgcga ctctaaatac agttggacac
     4621 acatacgagc aactgtatac cttttaatca gttttctata ttttatttat tatatctgtc
     4681 ttaatgataa aaattgttac aaacagttta acatatttag ctacctattt tgcatataaa
     4741 aaacatgctt gcatacacta tgcaataaaa attacaaatt tatatatgat accactatgc
     4801 ttgcttatct ctatagcgcc attgatacac atttttaaat atctatactg ccgttagaat
     4861 tttatcatgt cttaattttc attaaatatt aattacttca ttttatataa accaacaaaa
     4921 accccctcac tactatgcaa gtgagaggtt atgttgatgt gctttatttt catcaatgtc
     4981 attaacacaa tcatatttct tctgttaatg cactgtatta tattcaaacg cgctatcgac
     5041 aattaatttg aagctacaaa ggtaccgcat aatggcagca cattaattaa caatgcgcta
     5101 aactttaatg aggcgctgat ttgcttgtct ttaaaagtca acatagcact gataattcct
     5161 aaaatagcta ctatacgtgg aattgtcatg ctcggatact gcggtatatg taagaaagca
     5221 ccaagacctg ctatcacacc acatactaac gcaatatata gaaattgctt caaatcattc
     5281 actcctaaat tgttattaca ctattacaca tagctaatca tcaatgtgaa atcaccttca
     5341 aagacactat ccaaatcttc agaagtcaaa ataaagtttg taccagtagt cagtttgaaa
     5401 atttcaccat cgacaatcat ttgcccttcg ccttccaaca ctgtaactaa acagaactct
     5461 ctaggcttca tataatttaa cgtgccagaa atttcccatt taaccaatgt aaagaaatca
     5521 ttcgatacaa tgtgtgtaca cttatggttt tcaataattt cgctttcagg caaaatatta
     5581 ggtaatggtg cattgtactg aataacgtct aaagcttttt caatatttaa cggtctatca
     5641 ttatattgat tatcttgacg attgaaatca taaagtctat atgtaatgtc tgacgattgc
     5701 atcgtttcgt atgctaatat tcctgaagaa atagtatgta ctgttcctgc tggaataaaa
     5761 tagaattctc caggttttac tttaatatat ctaagtatcg actctatcgt tccgtgttga
     5821 acatgattcg caacttcttc tctagactct gctaatgtcc ctataactat ttctgcatct
     5881 tcttctgcat ctataatata ccaacattca gatttgccat attgcccgtt ttcatgctca
     5941 taagcataag aattatcagg gtgcacatga atagaaagtg attctcttgc atccactatt
     6001 ttagttagaa gcggaaaatc tttgcttggg aaatcaccaa acaattcacg atgttctgac
     6061 caaatacggt ctaatgtttg accttgatat ggtccattaa taatctcgct cgtaccattt
     6121 ggatgtgctg acacacacca acattcccca gttgtatcat tgtctaattg atatccaaac
     6181 tcacttagac gttgaccgcc ccataatttt gtttttaaaa ttggttgtaa aaataatggc
     6241 attgttgcac ctccattgtg attaagtaag caatagaact ctgatgttgt tgttccatta
     6301 tattttgatt ttgttctcat ttacatcgta ttattaactt ccacatttca aattaactat
     6361 tagtgattgt accatattta ctaacattgc agtactgcca attaaagcgc ttcacttaaa
     6421 tttacagtac tttaacattt tcaaaaattt atagcataga gattatatct ctcttacatt
     6481 tgtacatatt tccctttaaa tttactcgcc cattatacca attaataaac aactttaata
     6541 gttgtgccat acattgttca aattctttgt aaaacgcata gacaatacgt acttattcat
     6601 acttataatt catcattttc aaaaaataac gagttacgaa aaagtaaccc gcttcaaatc
     6661 atatttacta tccttattaa tccgtttcat tttcaaattg agttaaagca tctttaatgt
     6721 cctgatcacc actaataatt tgaaactctt ggtgattaaa atgattggat gtgacaattt
     6781 cttttaatac tgtcgcaaca tcttctctag gaatttcacc tttaccatca aaatattgtg
     6841 cagcttctat ctttccagat cctgctgcat ttgtaagtgc ccctggatgt aaaattgtat
     6901 aattcaaacc tgaacgtctt aaatagtcat cagcgtaatg tttagctatt gtatatggct
     6961 ttaaatcacc gctatcatca aaagcctgac gtctcgaatc atatgttgaa accatgacat
     7021 agtgtttaat attggcctct ttactcgcaa tcattgattt aacagcacca tctaaatcga
     7081 caataattgt tttatctgca cccgtgttcc ctccagaacc tactgaaaag ataactttat
     7141 cgaatggttt aaacgtctca gttaaagtct ctattgaatc attttcaaca tcaacaagaa
     7201 ttgctttcat accttgtgat tttaacgcat taagttgatc tgattgccta acaccagcag
     7261 taaatggtac attttctttt gctaattgtt gcactagtaa cgaacctaca ccgccattag
     7321 cacctataac caaaatattc atttacaaca ctctcctatt tattattctc tatgccatac
     7381 cactttatga gatatgtaaa acttgttaca actataaaaa tcaattgaca tactactggg
     7441 aacgtattaa attaatatat gaacaaatat tcacatgaaa ggattgtcat ataatgtcag
     7501 aacaatattc agaaataaat acagatacat tagaacgcgt aactgaaatt ttcaaggcat
     7561 taggcgatta caatcgaata cgtatcatgg aattgttatc agtcagcgaa gcaagtgttg
     7621 gtcacatttc acatcaattg aatttatctc aatcaaatgt ctcgcaccaa ttaaaattac
     7681 ttaaaagtgt gcatcttgtg aaagcaaaac gacaaggcca atcaatgatt tattcattag
     7741 atgacatcca cgtagcaact atgttaaagc aagccataca tcacgcgaat catcctaaag
     7801 aaagtgggtt ataatatgtc tcattcacat catcatcatg accatatgca tagtcatgta
     7861 actacaaata ataagaaagt attgtttata tcgtttttaa taatcggtct atatatgttt
     7921 atcgaaatca tcggcggtct ccttgctaac agcttggcat tactatctga cggtatccat
     7981 atgtttagcg acacattctc attaggtgtt gcacttgtcg catttattta tgctgaaaag
     8041 aatgccacaa ctacaaaaac atttggttat aaacgtttcg aagtactcgc agcgttattt
     8101 aacggtgtaa cgctttttgt aataagtatt ttgattgttt ttgaagcgat taaacgtttc
     8161 tttgttcctt ctgaagttca atcaaaagaa atgttaatca ttagtattat cggtttaatt
     8221 gtcaatatcg ttgttgcatt ctttatgttt aaaggcggcg acacttcaca caatttaaat
     8281 atgcgtggtg cttttctaca tgttatcgga gacttattag gttcagttgg cgccattact
     8341 gcagctattt taatttgggc atttggatgg acaatcgccg atcctatcgc aagtatttta
     8401 gtttccgtta ttattttaaa aagtgcttgg ggtatcacaa aatcttcaat taacatttta
     8461 atggaaggca caccaagtga tgttgatata gatgaagtta taactactat taaaaaggat
     8521 tcacgaatac aaagtgtgca tgattgccat gtttggacaa tttcaaatga tatgaatgca
     8581 ttgagttgtc atgttgttgt agaccataca ttgacaatga aagaatgtga attattatta
     8641 gaaaacattg agcatgattt attacattta aatattcacc atatgactat tcaattagaa
     8701 acgcctaatc acaaacatga tgaatcgatt atatgttcag gaacacatag tcattcacat
     8761 aaccatcatg ctcatcatca cgcgcatgta cattaataat tttaacctac tgccattgca
     8821 tcgattaaac ttttcaatgg cagtaggttt tttatgtctt tatggcgact tgtttggtct
     8881 ttgatgatgc aatgtttatt aacaaatttt caactattat ttcttacatt agtcatattt
     8941 ttgacaattt actattataa ttctctaact ttagtcactt taattaattt ttattagata
     9001 ttaatatgaa aataacgtgt tttttgttat tgtatttata tgaagaaaag gaggcaccac
     9061 tttgtttact tactttaaat cagcatttaa aaatgctaaa ccacaattat taataacatt
     9121 gatatatgca cttatagcat ttgctgtaat tgcagttgta tatttacttg ctaatttcca
     9181 gcttgctaag tatgcacaaa ctattgcaat ttattcacaa tttgggcaaa aaccaccagt
     9241 agatgcatat ttaaaagtta tcgctgtatt acttatagca gctgtagttt cactgtttgt
     9301 tttagttcaa atttttatcg gaattacaaa tgttatgaaa cgagcaatga gtcatgaaaa
     9361 agtaaaattt accgatttat ttattgcatt taaaaaaggt aattatttaa agagcgttct
     9421 gatcggtctt gtatctacag caatgattat cgttctttca cttctgacat cattactata
     9481 caaattattt tctccagtat cagaaatgat tatgaattca gtacaatcat catatgcaga
     9541 tagtacacac ctgattggta ttgctattac aactcaaagc atcataatca ttgttgtttt
     9601 attgattaaa gcaattatta cttggttatt gttaatacct attttcaact ttatgactag
     9661 tttcgttgaa tcaactaacg ataaagtaaa aacacactta gctaatggat ttaaagcaat
     9721 gaaaaatggt caaaaaacat ttttcaaatt t
//
LOCUS       DALAGF010000101         9679 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.101, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000101 DALAGF010000000
VERSION     DALAGF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9679
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.101"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(122..2098)
                     /gene="sfaD"
                     /locus_tag="O2692_002231"
                     /gene_synonym="sfnaD"
     CDS             complement(122..2098)
                     /gene="sfaD"
                     /locus_tag="O2692_002231"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HCZ4299844.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPLIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWVETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            2200..3393
                     /gene="sfaA"
                     /locus_tag="O2692_002232"
     CDS             2200..3393
                     /gene="sfaA"
                     /locus_tag="O2692_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HCZ4299845.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDASRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            3380..5137
                     /gene="sfaB"
                     /locus_tag="O2692_002233"
     CDS             3380..5137
                     /gene="sfaB"
                     /locus_tag="O2692_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HCZ4299846.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            5144..6214
                     /gene="sfaC"
                     /locus_tag="O2692_002234"
     CDS             5144..6214
                     /gene="sfaC"
                     /locus_tag="O2692_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HCZ4299847.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNHY"
     gene            complement(6315..6440)
                     /locus_tag="O2692_002235"
     CDS             complement(6315..6440)
                     /locus_tag="O2692_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299848.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
     gene            6606..7589
                     /locus_tag="O2692_002236"
     CDS             6606..7589
                     /locus_tag="O2692_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ4299849.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            7601..8632
                     /locus_tag="O2692_002237"
     CDS             7601..8632
                     /locus_tag="O2692_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCZ4299850.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIIHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPALEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWIIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            8629..9597
                     /locus_tag="O2692_002238"
     CDS             8629..9597
                     /locus_tag="O2692_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCZ4299851.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MTFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
BASE COUNT         3267 a         1354 c         1745 g         3313 t
ORIGIN      
        1 cctgtttttt gtgattttta ataatttgaa tatggaaaat gtattattct ctcatttgta
       61 tagattgtat ttaataagtt aatgtaatcc ttgagctcac gattaataaa attctataac
      121 cttaattatt ttctcgatac aaagggttat taactttaat ataagtataa tgatgtgcct
      181 catcttcaag acgcattgtt gtaacacact tataatctat aaatggcgcg aacatggtat
      241 ctttaatttc atttatgcga ttctcattta cttgattaga tttgtgtgtc gaaagtacaa
      301 gttgatcaaa aatgttatct agtacatcac gaacgatata ccacatatgt ctttctaagt
      361 ttgaatcatt tgatgcttta gagattgtaa gaattaattc gcctaaatgg ttttgaacgg
      421 tagaataaaa ggctttgtta aacacagacg tttttgaatc agtaagaatt cttgattttt
      481 catggaaatg agatgtactg tatcccattt catttagttg tgctttatca attcttaaac
      541 cttcaaagtc tctaatatac atcgtgtcca atagaccatc tttccgaaat gttgcaatag
      601 cattttgcaa gtgagcctct aatgcaatgc catatttagt aactagtggg attacgagac
      661 caagcaatgc tttactataa gtttcaaccc acgatttcgc cgatgattca aaatcagaca
      721 atgatgcagc tgattgataa cgtttaatca atgtcacgat aggtgattca ttgttaaatg
      781 ggtaggttgc aactaagctt gaaggaatca atggtgtgac ttcttgtgga atcatttggt
      841 atatattttt tctaaataat gtacctaatt gttcacttcg ttcagtttga tagtctgctt
      901 cgtcttgctc attataaaaa tgaataccag caacttcatc aataattgtt gatgcatagg
      961 acttaaatat gacatctttc tccaaaatat catttaaaat acgtgtcatt agtggaccat
     1021 tgtgcgtcgt ttgttctgat aatgtacgaa tctcacctgt aatatgaacg tttgtcgaca
     1081 atttgatgtg tggcgacata gctgggtatt taggaactaa tgttctgaaa gataaaccag
     1141 cataataatc caacgtatgt tttgcttcaa tgattaattc tttatctact tctgcttgat
     1201 aatcagaatg taatacgtca tctaattgcc atggatgaac aatcataatg tgataatcat
     1261 taaagttaaa ctttggcgta aattcatttt ctaattgttt aattaagtcc ggaaatagtt
     1321 gatgaacagt tgtatcataa tctttagaca gtgacatagt acggcttaat ttactgtgaa
     1381 tcagtactat tttcaactta ataggttgat tgaattctga agaatatagg aatgtttgta
     1441 atgcatttaa acctttacgt aatttagccc caggatgtag cggatgacct tcaataacgg
     1501 cttgctctga acgcaagtaa ctatcttcgc tattttcgat aatattaaat aaaggtgcag
     1561 aatcatgttg cattgacagt gcttgatagc taattgcaaa tgtcatatta gttgcactgt
     1621 ttattaaatc ttgctgaaat tgatcactag cagcattttt taaatctggt gcttcaatta
     1681 aaatacactc aagaatttca tttggatggt gtactcgtgt aatcgtattt gtaatgtcat
     1741 ctttaatgta gaaagggcct tcaacatcaa ttcgatcaaa ggcgtgttct ccagtgatag
     1801 gagcatataa tgtttgctta gcttgtggga actggatttc tagtatatga gtcgttgaga
     1861 tatctaacat aatcaaatca cgactcaata ttttcttact ttgagtgcgc gctttaacta
     1921 agttttcgcg atgcattgat gtgaccaatc tctgagtgac tttatctctt ccttgtaaaa
     1981 tcatctcttt aaaaatatta gcccaatcgc tattatgttg ttgtaaaaat aaatatgttt
     2041 cttgttcttc tttgttaaat tttaatgtct gttctttaaa aattaagttc aagttcataa
     2101 ttcacctcta tgaaatattt tacaaaagca agatagattt gtataatcca tattaatgat
     2161 aatgattctt attatcaaca gaatgcgggt gtaagtttta tgacaaaata tttttttagc
     2221 agttcttttc tactatttct aggtaattgg attggacaaa tagggctaaa ttggtttgta
     2281 cttaccactt atcataacgc agtttatctg gggattgtca atttttgcag acttgtacca
     2341 atattattac taagtgtgtg ggcaggggca attgccgata aatatgataa agggcgattg
     2401 ctgagaatta caatttcatc atcattttta gtaactgcaa ttttatgtgt gctcacgtat
     2461 agtttcactg caattccaat tagcgtcatt attatatatg cgacattaag agggatttta
     2521 agtgcggttg aaacaccttt aagacaagca atcttaccag atttatcaga taaaatatct
     2581 actacacaag ctgtatcatt tcattcattc atcattaata tttgtcgttc aatagggcct
     2641 gccattgctg gtgtcatatt agcagtctat catgcgccaa caacatttct tgcacaggca
     2701 atttgttatt ttatcgcagt tttattatgc ttaccattac attttaaagt aactaaaata
     2761 cctgaagatg catcaagata catgccgtta aaagttatta tagattactt caaattacat
     2821 atggaaggtc gacaaatatt tataacatca ttattgatta tggcgacagg tttttcatat
     2881 acgacacttt taccagtttt gacaaacaaa gtatttccgg ggaaatctga aatatttggt
     2941 atcgctatga cgatgtgtgc cattggtggt attattgcaa cgctagtttt acctaaagta
     3001 cttaaatata ttggtatggt aaatatgtat tatttaagtt catttttatt tggcattgct
     3061 ttgttaggtg tggtatttca caatattgtc atcatgttca tttgtattac attgattggg
     3121 ttatttagtc aatgggcacg tacgacaaat cgcgtttatt ttcaaaataa tgttaaagat
     3181 tatgaacgtg gtaaagtact gagtattatt atgatggata gaggtatgat tccattggga
     3241 agtctattaa tgagtatatg tgcagatgtg tttggcattg ttagaacttt ttcaataatg
     3301 ggaataagta ctatatgcat tacaatggta ttctatttta taaatagaaa gttgaagtta
     3361 aagttggagg aaagtaatca tggtatatct tgaatgggca aaggcagata gaaatattca
     3421 atatcgtgta attaacgcca ttattaaaga acgtatttac cccgagcaaa catttatttc
     3481 gcaaaaagga tctttaattg aaattcagta tcatatgcat gtgttgacta ttgaagttgt
     3541 tagaaaaagt gcattagaac gctatgagtt tacaggtgat attacttatt taaataaagg
     3601 tgaaacgtca ttaattataa ctttagaagg tttattagat gtgttgaatc atgactttga
     3661 tatccctatt tcagagcgac tacgcgaaga gttaatacac agtcgagata gtttagttga
     3721 aacatataag caaatgtctc acagacaaac gttaataagt caaagtttta aattttcaag
     3781 gttaccacaa gatattaact ttttttcatg gttacaacat gtaaaagata gtgataagac
     3841 agatgattta acttattctg agagtttggt accagagggg catccaacac accctttaac
     3901 caaaacgaaa ttgcccttaa ctatggaaga agtacgagca tatgcacctg agtttgaaaa
     3961 agaaatccct ttgcaaatta tgatgattga aaaagaccat gttgtgtgca cagctatgga
     4021 tggtaatgat caatttatta ttgatgaaat aattcccgaa tactacaatc agattcgtgt
     4081 gtttttaaag agtttaggtt tgaaaagtga agactataga gcgattttag tacatccttg
     4141 gcaatatgat catacgatag ggaaatattt tgaagcatgg attgctaaaa aaatattaat
     4201 tccaacgccg tttacaatac tttcaaaagc aactttatca tttaggacga tgtctttaat
     4261 tgataaacca taccatgtta agttgcccgt cgatgcacaa gcaacaagtg ccgttagaac
     4321 agtctcaact gtgactactg tagatggacc aaagttaagt tatgctttac aaaacatgtt
     4381 gaatcaatat ccaggattta aagttgctat ggaaccgttc ggtgaatatg caaatgttga
     4441 taaagatagg gcacgtcagc ttgcttgtat tataagacaa aagcctgaaa ttgatggaaa
     4501 aggtgcaaca gtagttagtg caagtctagt taataaaaat ccaatagatc aaaaagttat
     4561 cgtggatagt tacttagagt ggttaaatca aggaattact aaagaaagta ttacgacatt
     4621 tattgaacga tacgctcaag cattaatccc gcctttaatt gcttttattc aaaattatgg
     4681 aattgcttta gaagcacaca tgcaaaatac agtagtgaac ttggggccac attttgacat
     4741 tcaattttta gtgagagatt taggtggttc tagaattgat ttagaaacat tacaacatcg
     4801 tgtatcagat attaaaatta caaatgatag tttaatagct gattctatag atgcagtgat
     4861 tgcaaaattc caacatgctg ttattcaaaa tcaaatggca gaattaatcc atcattttaa
     4921 tcagtatgat tgtgttgaag aaaccgaatt atttaacata gtacagcaag tagtagcgca
     4981 tgccattaac ccaacactac cacatgcaaa tgagttaaaa gatattttgt ttggaccaac
     5041 aattactgtc aaagcgttgt taaatatgag aatggaaaat aaagtaaagc aatatttaaa
     5101 tattgagtta gataatccga taaaaaaaga ggtgtagtac tacatggcac acgttaacat
     5161 aaatatatcg aagattaaat ataacgccaa agtacttcaa acagtttttc aaagtaaaaa
     5221 tatgcaattc acaccagtaa ttaagtgcat agctggtgac cgtacaattg tagaaagctt
     5281 aaaagcgtta ggtatcaatc atgttgcaga atccagattg gataacataa ttagtattgc
     5341 agatcaggat ttaacatata cgttattgcg tacgcctgcc aaaaaagaga tttcagatat
     5401 gatagaaaaa gttgatatga gtatccaaac agaattatct accatacatc aaataaatga
     5461 agtagcagaa gttttaggaa aaaaacataa gattttatta atggtagatt ggaaagatgg
     5521 ccgtgaaggt gttctgactt atgatgtttt agattatatt aaagagatta ttcacttgaa
     5581 aaatatccat tttgtaggtt tagcattcaa ttttatgtgt tttaaatctg atgcgccaag
     5641 tgatgatgat atctttatga ttaatcgatt cgtctcagct gttgaaagag aaataggata
     5701 tcgattgaaa attatttcag gtggtaattc gagcatgtta ccacaacttt tatataatga
     5761 tttgggtaaa ataaatgaat tgcgaatagg tgaaacgttg tttagaggtg ttgatacaac
     5821 gacaaatcaa gcgatcgcta tgttatatca agatgcgatt acattggagg ctgagatttt
     5881 agaaattaaa cctcgagtta atacacaaac gcatgaatca ttcttacaag ctattgtaga
     5941 tattggttat ttagatacaa aagtcgataa tatttcacct atggatcagc atatcaatat
     6001 tttaggcgct tcgagtgatc atttaatgct tgatttaaat ggtcaaggac actatcaagt
     6061 tggagatcat atatcttttt cattaaatta tgaagcactt tctcattcga tgtatatgaa
     6121 aaatttacat aaggtatata ttgatgattc aaagattgat acgctacttc aaaattttga
     6181 tgtgaaatct cctgctatgg taaaccacta ttaatatatt tatcaataag tctaagttga
     6241 caagttatac tacatatcgt ttgcacggtt gtatcataat tgttcaactt agattttttg
     6301 tatttgttga tttatcaaat taagtgcaac agttcgtcac ataaaattgc aacagataat
     6361 atcagctgaa ttacagggat aacggtcatg ctaaatggtg tcaattgtat taatgcaaag
     6421 atgatatagc aatgatacat tatcagtatt ttgtctaagg aaatgtgcta attgtagtca
     6481 taattattag aggaaatata tagtcataca ttttagaaat acaaaaaaga ttgaacgcta
     6541 cttgacaatg ataattgtta tcaataaaat aataaatgaa gttatacata ttaaggagtg
     6601 gaacgatgag aggtctaaaa acttttagta tattgggatt aatagttgcc ttacttttag
     6661 ttgcagcttg tggtaatacg gataattcaa gtaaaaaaga atcatcaact aaagatacta
     6721 tttcggtaaa agatgaaaat ggtacagtaa aagtacctaa agatgcaaaa cgtatcgttg
     6781 tattagagta ctcatttgca gatgcattag cagcattaga cgttaaacca gttggtattg
     6841 ctgatgatgg taagaaaaaa cgtatcatta aaccagttag agaaaaaatt ggggattata
     6901 cttctgtagg tacacgtaaa cagccaaact tagaagaaat tagtaaatta aaaccggatt
     6961 taattatcgc tgatagcagt agacataaag gtattaataa agaattaaac aaaattgcac
     7021 caacattatc attaaagagt tttgatggag actacaaaca aaatattaat tcgttcaaaa
     7081 caattgctaa agctttaaat aaagaaaaag aaggcgaaaa acgtcttgct gagcatgata
     7141 aattaatcaa taagtataaa gatgaaatta aatttgatag aaatcaaaaa gtgcttccag
     7201 cagtagttgc taaagctggt ttattagcac atccaaacta ttcatatgtt ggacaatttt
     7261 taaacgaact aggatttaaa aatgcattaa gtgacgatgt aacaaaaggt ttaagtaaat
     7321 atttgaaagg accttactta caattagaca ctgaacattt agctgattta aatccagagc
     7381 gtatgatcat tatgacagat catgctaaaa aagattctgc tgaattcaag aagttacaag
     7441 aagatgcaac atggaaaaag ttgaatgcag ttaaaaataa tcgcgtggat attgttgacc
     7501 gtgatgtttg ggcaagatct cgtggcttaa tttcttctga agaaatggct aaagaacttg
     7561 ttgaattatc aaaaaaagaa caaaagtaag gtggaagtaa atggctataa aagaaataag
     7621 tagccaatct gccatagatc ataaaagaaa aagacgcaca acactcacgt atatagtgag
     7681 tttgtgcttt ctttttattt gtatatattt aaatatggcg attggttctt cgaaaattaa
     7741 ttttagcgat atcattcact atgttactgg tcatacagat acgaaagcaa cgtttttatt
     7801 gcataatgta cgtatgccaa ggatgattgc agggttattt attggcggtg cattagcggt
     7861 atctggtttg ttaatgcaag caatgacacg caatccgcta gcatcaccta aaatttttgg
     7921 tgtcagttct ggtgcatcgt ttttcattgt atttgtgacg attatcattc cagcattaga
     7981 atattatgca ttatatttag gtgtgatagg tgcatttata ggtggtttaa ctgtttatac
     8041 actttcaggt gctacgaaag gtatgacacc cattaaatta gcattggctg gtatggccat
     8101 ccatttgttt tttagtagta tgaccgaagg tattattatt ttgaatgaga attcaaacga
     8161 acaagtgatg ttttggcttg tcggttcatt atccagtatg aagtgggatg aaattttaac
     8221 cattctacca tggattattg gtgctttaat tgtaacgatt tttattggac gtcaactaac
     8281 gataatggaa ctaggcgatg acattgctaa gggattgggt caaaatataa ataaagttag
     8341 aattatcatt ggactattag tcattattct tacaggtatg tcggtgtcag tggcaggacc
     8401 aattggattt gttggcttaa tcgtaccgca tatagtcaaa cgctatgtga gtaagaatta
     8461 tttagtcatg attccattaa catttattat aggtgcagat ttgttactac tatcagatgt
     8521 attaagtcgt ttaattacat atccttatga atcaccagta gggattgtta catcattcgt
     8581 aggtgcactt tacttcttat ttataacgat taaaggggtg aaaagaatat gatgacaacg
     8641 aaaattatac gtcgttatag tttaatcaca atccttttaa ttgtgagtat attcataagt
     8701 ttatgtgtag ggtctgtcat gattcatccg attgatgcta ttaagggaat ctttacacaa
     8761 gatgatttta ttttaaatga gtatcgtata cctagaacat tacttggtat tattatcggt
     8821 agtagcttgg ctatttcagg tgcaattata caagctgtaa taagaaaccc gttagcctca
     8881 cctgatgtta taggtatttc taaaggtgcc agtttagctg ctgtgattat tattatgacc
     8941 tttccaacag caccattgtt tgtattacct ataggttcat tcttaggtgc ctttgcagta
     9001 agtttatttt tatcattttt aatttcaaaa tatgatgtta aaggttcaaa gcttgcctta
     9061 attggtttag caataggcgc aatatgtacg gcgattgtgc agttcttatt aatcagaaat
     9121 ccactggaag caaacaaagc gcttgtttgg ttgacaggta gtctatatgg acatgatatg
     9181 tcgaatattt atactatatt accttggttt ttaataacga taccaatcat cattttattg
     9241 agttatcaat tagatgtatt gaatctaggt gatgctgttg caaccgcact cggtttaaaa
     9301 gttaaaacta ttaaaatgct attgttaatt ttagcagtaa tattagcagg atccgcaatt
     9361 tcagttgtcg gtggtattag ctttttagga ttaattgcac ctcatattgc gagacaatta
     9421 ataggaaata aaacattaca cgttattatc atgtcggggt taattggtgc tatattgtta
     9481 acatttggag atggtttagc ccgtggcatt catccgccac tggatattcc tgttggggtt
     9541 atcattgcaa ttatcggtgc accatacttc ttaatattat tacgaagaat gaaataacta
     9601 acatatgatt agagtttaaa aaaggttaag tacgaaatca gtaattgtac ttaacctttt
     9661 ttgaattcat cttgaaaaa
//
LOCUS       DALAGF010000102         9661 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.102, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000102 DALAGF010000000
VERSION     DALAGF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9661
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.102"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(125..1933)
                     /locus_tag="O2692_002239"
     CDS             complement(125..1933)
                     /locus_tag="O2692_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCZ4299852.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     ITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASE
                     LASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLQSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELAN
                     KSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGFEGKKKI
                     NAIMTKFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS"
     gene            2050..2442
                     /locus_tag="O2692_002240"
     CDS             2050..2442
                     /locus_tag="O2692_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HCZ4299853.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEKMSLPLMWEFPGGKVEKNE
                     TEKDALIREIREEMKCDLIVGDKVITTEHEYDFGIVRLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            2444..5305
                     /locus_tag="O2692_002241"
     CDS             2444..5305
                     /locus_tag="O2692_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HCZ4299854.1"
                     /translation="MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLIL
                     KAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKN
                     LVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLNEVKDITEDDIDTSLRILDF
                     SFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNMFLEDLIELSKYNNEKYQK
                     GKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPIFITYDKHEDISDSTKY
                     EDEFLSQDELKWFTKSNRTLESKEVQKILSHRAKGIKMYIFVQKKDDDGIYFYYLGTA
                     GYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN"
     gene            5611..6384
                     /locus_tag="O2692_002242"
     CDS             5611..6384
                     /locus_tag="O2692_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ4299855.1"
                     /translation="MIHSKRLRLWLYLVLLAVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDQEFEKGDKGTWIIHSKMTIETNGKNMESRGLVLYVDRNT
                     RTTKGEFIVRELWEDKKGYSRSKEKEYPVKMEHNKIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNDDYNVKQLRKRYDIPTKKAPKLLIK
                     GDGDLKGSSIGHKNLEFTFIENKEENIYFTDSINFKPTE"
     gene            6496..7280
                     /locus_tag="O2692_002243"
                     /pseudo
     CDS             6496..7280
                     /locus_tag="O2692_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            7277..8601
                     /locus_tag="O2692_002244"
                     /pseudo
     CDS             7277..8601
                     /locus_tag="O2692_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8601..8927
                     /locus_tag="O2692_002245"
     CDS             8601..8927
                     /locus_tag="O2692_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299856.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQIDELRHQTRKKCEQMY
                     DHFLYLKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            8930..9217
                     /locus_tag="O2692_002246"
     CDS             8930..9217
                     /locus_tag="O2692_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299857.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKKFEDDFKNAAQGAWVKNATDELKDISNDLEKIQDIKV"
     gene            9534..9641
                     /locus_tag="O2692_002247"
                     /pseudo
     CDS             9534..9641
                     /locus_tag="O2692_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
BASE COUNT         3768 a         1230 c         1694 g         2969 t
ORIGIN      
        1 aatatacttt tgaaaaatta ttacaaatta gccccttcaa aacgcgaaaa cataaggatt
       61 ctagtttcaa aagggctgat aagcataaaa tgaaatgtaa tatttcgatg tataaaattt
      121 ttaattagct aaaaatcatc gcattaattt tttgagctac atcatcaaaa ttcggacatt
      181 ttaacgacac atataattta attttaggtt cagtaccaga aggacgtaaa gcgataaatc
      241 cttcgtcaaa taagacacga ataacatttg atttaggaga attaatctgc gacgttgtat
      301 ctttatccaa atgataaacc tcgctagtta aataatcttc aattgctttc actttgagtc
      361 cttgaatctc ttgcggtgga tttgaacgga atttggtcat tattgcatta attttctttt
      421 tcccttcaaa tccttctagc gtatgcgaga ataatgtatc ctcatgtcta ccaacagttt
      481 gataaatctg ttctaattca tctttcaatg ttttgccata taattttaac tcagaagcgt
      541 attttataat gagtggcaca atttgtacgg catctttatc acgtacaaaa ggctctgata
      601 gaaaaccgta actctcttca aatgcgaaaa tcatattttg atgatcatcc agttgtctta
      661 tttcctgagc aataaattta aagcccgtca gcacctcttt gtattcaaca ttattatagc
      721 gtgctaatga ctttgttaac tcactactta caatagattg aatcattaag cggtgacgta
      781 attgagacgt ttgttgaata cgataattaa gtaacagcgc accaatttgg ttgccattaa
      841 aatatgtgat atgaccgtgt gcatcacgtt cagcaatacc aagtcgatct gcatcagggt
      901 cagttgatat taataagtct gcatgacttt tatttgcaag ttcgactgct tgatcaaaag
      961 cgcgatgatc ttcaggatta gcactttgaa ctgagctgaa attcggatca ggtttacatt
     1021 gtgcttctac aagattaaat tgattgaaat tcaaagattg taataactca ggtacaatcg
     1081 gaacacttgt accatgcaaa ctagtgaaca caacttgtaa atcagattta ggaatatagc
     1141 caatcatatt ttgaatgtgt ttcatatagt catcagttac agatttcgga aaaggcttga
     1201 tataagatgt attttgttta gaaataggta tatcaatctg taatggatcg ccaacttctt
     1261 cgatataacg acttgcaagc tcagatgcat cagtcgataa ttgcgcacca tcagaaccat
     1321 atactttgat gccgttatag tctttcggat tatgacttgc tgtaatcata atgccagcag
     1381 tagtattaag attgcgcacc gcgaacgata attccggtgt cgttttatat gtatcaggta
     1441 aataaactgt tatttgatgg tttgctaata cattagcaat tatttgggcg aattcagttg
     1501 aaagatgtct aatatcataa tgaatgacta ttgttggact gtttgtttgg gcatttaaat
     1561 aacgcgctaa acctaatgcc aatttttcaa tagtaaactt attaagtcga ccttcaccaa
     1621 gaccgaattt tccgcgtata cccgcagtac caaatgttaa tttactttca aaaccttctc
     1681 gctgttcaat gtcagattgc tgctcataaa aatgtttaac taaactatca ttagctcgtt
     1741 ctatccataa ttctttatcc attgttgcta aacatccttt caaaatctca gttagactta
     1801 ataaaacatg aaaactaaag cccttacatt tatgtaatga attataaaga aatacgcccc
     1861 aaaagtaaaa aaacacagcc ccaagacaat acttttcaca agtattatat aatagatgtg
     1921 tatgaaaatg catggagtag agcgttcatg tgaatgtaaa atattaattt ttaatattga
     1981 tttatatata gttttgtaat ttttgataga ataacaatta agatgatttt aaattgaagg
     2041 gtgagtctta tgaaaaaagt aatcaatgta gtaggagcta ttattttttc tgataacaaa
     2101 attctttgtg cacagagaag tgaaaaaatg agtctgcctt taatgtggga atttcctggc
     2161 ggtaaggttg aaaagaatga aactgaaaaa gacgctttga ttagagaaat tagagaagaa
     2221 atgaaatgtg atttaattgt tggagacaaa gttataacta cagaacatga atatgatttt
     2281 ggaattgtta ggttaacaac atacaaatgt actttaaaca aagagttacc aactctaact
     2341 gaacataaga gtattgaatg gttgtcaata aacgaacttg ataaattaaa ttgggcccca
     2401 gcggatatac cagccgttaa taaaattatg actgagggat aatatgagta gattactaaa
     2461 tgatttcaat caatctttac ataaagggtt tatagacaaa catatttctc ataaagggaa
     2521 ttatacaccg aagcttttag taaataataa aaatgaaaaa gttctttcaa caattataga
     2581 tgaacttcaa aaatgtgaaa cgttttattt ttctgtggct tttataacag aaagcggttt
     2641 agccagctta aaggctcaac tattagattt aagcaataag ggtgttaaag ggaaaatatt
     2701 aacatctaat tacttagggt ttaatagtcc taaaatgtat ggagaattac ttaaattaaa
     2761 aaatgtagag gtaagattaa ctgatattgc tggattccat gccaaaggat atatttttga
     2821 gcataaggat tatagttcta tggtaatagg aagctctaat ttaacatcta atgcgttaaa
     2881 ggttaattat gagcataatg ttttactgtc tactatgaaa aatggcgatt tagttgatag
     2941 tgtaaaaagt gaatttgact tgttatggca aaagagtacc ccactgactg agcaatggat
     3001 taattcatat aaagaatcat ttgagtaccg ttccttagaa aaattagcgg aggttgaaca
     3061 aactcaaatg ctgctagctg ataaggtgaa aaaatcagtg gaaattgtac ccaatttaat
     3121 gcaagcagaa gcattaaggt cattaaaagc tattagggat aaagccaaag ataaggcgtt
     3181 aataatatct gcaactggta caggtaaaac gattttatgt gcattagatg ttagagaagt
     3241 aaaccctaat aaatttttat ttattgttca taatgagggg attttaaata gagctaagga
     3301 agaatttaaa aaagtattac ctataaaaaa tgatagtgat tttggattgt tgacaggaaa
     3361 acatagagat gtcgatgcca aatatttatt tgcaaccatt caaacattat ctagagatga
     3421 taattttaag caatttgatg aaaatgaatt tgattatatt gtttttgatg aagcacatcg
     3481 ttctgctgca tctacttatc aacgagtatt taactacttc aaacctaagt tcatgctggg
     3541 aatgactgct acaccagaaa gatcagatga attaagtata tttgaactgt ttgattataa
     3601 tattgcatat gaaataaggt tacaagcagc attagaaagt gatattttat gtccatttca
     3661 ttattttggt gtgactgatt atgtacatca aggtattaaa gaagatgatg taactaaact
     3721 gagatattta acttctgatg aaagagttaa ttatattatt caaaagacag attactatgg
     3781 atattcaggt gaaattttac aaggattgat ttttgtaagt agcaaaaaag aagcttatga
     3841 tttagcggat aaactaagtt caaaagggat taaaagtgtt gctttaacag gagatgatag
     3901 tgttaattat cgccaaattg taatagaaaa attaaaagaa ggtaagatta actatattat
     3961 aactgtagat ctttttaacg aaggtattga tattccagaa gtcaatcaag ttgtaatgtt
     4021 gagaccaaca gagtctagta ttatattcat tcagcaactt ggtagaggat taagaaaaag
     4081 ttcaaataaa gagtatgtta cagtaattga ttttattggt aattataaga caaattattt
     4141 aattccgatt gcgctttctg gggatcaatc gcaaaataaa gataattata agaagttttt
     4201 aacgaataac gattcgatta atggagtatc tacaattaat tttgaagaag ttgctaaaaa
     4261 acagatttac aattcattgg atgcagtatc attaaatcaa aataaattaa tattaaaagc
     4321 ttatgaagaa gttgaaaata gattgggaca catgccgtta ctaatggatt tcatacaaca
     4381 acattctata gatccaagcg ttatattttc taaatttagt aattattacg agttcttagt
     4441 gagatataaa aaaatagata cattattgac ggaaaatgaa tctaaaaatc tggttttctt
     4501 ttcaaggcaa atagcacctg gattgaaaag aatcgatagt ttagttttag aagaactttt
     4561 aaaaaatgaa ttaacatatg atgaattaaa aaataaaatg ttgaacgaag ttaaggatat
     4621 aacagaagat gatatagata cttcattaag aattttagat ttttcatttt acaacgcagg
     4681 aattgaaaaa atctatggaa gtcccattat agaacgtaat gaaagaatga taagactaag
     4741 cgatgcattt actaatgctc taagtaatca aacatttaat atgtttttag aagatcttat
     4801 tgaattatca aagtataaca atgaaaaata ccaaaaaggt aaaaatggac tgattttata
     4861 taataaatat tcaagagaag attttagtaa aatatttaat tggaataaaa atggttcgtc
     4921 tgtaatcatg ggatacatga taaaaagcca agaaatgcca atatttatca cttatgataa
     4981 gcatgaggat ataagtgata gcacaaaata tgaagatgaa tttttaagtc aagatgaatt
     5041 aaaatggttt actaaatcta atagaacgct agaatcaaaa gaagttcaga aaattttgtc
     5101 ccatagagca aaaggaatta aaatgtatat ttttgtacag aaaaaagatg atgatggtat
     5161 atatttttat tatttaggaa ctgctggata tatagaaggt tcagaaaagc aagataaaat
     5221 gccgaatggt tcaaacgtag taacgatgga tttagcactg gataaagctg taagagatga
     5281 tatatatcgt tatctaacaa attaatgtaa accgaaaaat gaatgttagt aaagttgtat
     5341 ttaaacatca attttaataa gtaaatacat tgagtttcag tgttattatt ttaaatataa
     5401 gcgcaattta tcttaattaa ttagttggtt aggccaataa aaattataaa gatatttgcg
     5461 ctttttaaat aatttgtgat gttgatatgt gaaaaataca actaacaatt aaaatccacg
     5521 aggatttaat cataaaattt cctctaatat tattaatatt ggaatttgaa cttaactttg
     5581 catcaaaaat aaaaatgggg atgtgaaatg atgattcact caaaaaggtt gaggctctgg
     5641 ctgtatttag tattattagc tgtttttata ggggcttgcg gaatgaaaaa ggaagaaagc
     5701 agtaaagata aacaaattaa agaaaacttc aacaaaacgt tgagcctgta tccaactaaa
     5761 aatcttgaag acttttatga taaggaaggc tttcgagatc aagagtttga aaagggcgac
     5821 aaaggcactt ggataattca ctctaaaatg acaatagaaa caaatggaaa gaatatggaa
     5881 tccagaggat tggtcctgta tgtagaccgc aataccagaa cgacaaaagg tgaatttatt
     5941 gtgagggaat tatgggaaga taaaaaagga tactcacgta gtaaagaaaa agaatatcca
     6001 gtaaaaatgg aacataataa aattatccca accaaaccga tagctgatga taaattaaga
     6061 aaagaaatcg aaaactttaa attctttgta caatacggtg actttaaaga tattaatgat
     6121 tataaagatg gtgacatttc atataatccg aatgtgccaa gttattcagc aaagtatcaa
     6181 ttaagtaatg atgactacaa tgtgaagcaa cttagaaaga ggtatgatat accaactaag
     6241 aaagcaccta aattattaat aaaaggcgat ggtgatttaa aaggatcatc tataggacac
     6301 aaaaatttag agtttacatt tatagaaaat aaagaagaaa acatctattt tacggatagt
     6361 attaatttca aacctactga atagggttaa tagaagtttg aaatgaaaat ttcataaagt
     6421 gttcaaaata atttttcgct ttagttgtgt aaattaaaga ttgagtcagt attaaaaata
     6481 taaggtggag aaatgatgat tcattcaaag aaattaacgc tcggtatatg tttggtatta
     6541 ctcattatat tgattggagg ttgtgtcatt atgacaaaaa caaatggtcg aaacgctcaa
     6601 attaaagaaa atttcaacaa aacattaagt gtatatccaa ccaagaatct cgatgatttt
     6661 tacgataagg aaggttttcg agatcaagaa tttgataaaa gagataaagg gacttggatt
     6721 atttattctg aaatggttat cgaaccaaaa gggaaaaata tggaatcgag aggaatggtg
     6781 ctctatatca atcgcaatac tagaacaacg aagggtaatt ttattgtcac cgaaataact
     6841 gaagatagta aaggatattc acgtagtaaa gaaaaaaata tcctgtcaag atggaaaata
     6901 atcgaattat tccaacaaag cctataccgg atgacaagtt aaaaaaagag attgaaaact
     6961 ttaagttctt tgtacaatat ggaaatttta aagattttaa agattataaa aatggtgata
     7021 tttcatataa tcctaatgtg ccaagttatt ctgcaaagta tcaattgaat aatgatgact
     7081 ataatgttca acagttaaga aaacgatatc atattccaac caaacaagcg cccgaattaa
     7141 aattgaaagg atccggcaat ttaaaaggct catccgtagg atctaaggat ctagaattta
     7201 cgtttgtaga aaatcaagaa gagaatatct atttttcaga ttcggtcgaa tttacaccta
     7261 gcgaggatga taaatcatga gtcaaacgga atatcaaata aaatctggca atataaaagg
     7321 taactctgaa gaaacaagta cagtatctaa tataagttat gaaatagaaa acgcaaataa
     7381 cagtggttta aaacaaaata aaattgataa acaaattaaa aagttacaag aaaaaaataa
     7441 attccctaaa aatctttcat atcttaaaag ttatacggac cccaaaacag gcacgactac
     7501 aagcgccttt ttaaataaag acactggcaa agttacttta ggtatgacag gtactaatgt
     7561 acacaaagac gcaatattaa aacaaacatt tggtgttcct tcttatcaag gatatataga
     7621 tgtgagtgaa acgctaaagg atattggggc cgatgtcaat attggccttc attccgtcac
     7681 agataaagat ccacattata aaaataccca agactttatc aaaaatatca aaaaagacta
     7741 tgatattgat attattaccg gacattcgct gggcggtaga gatgcgatga ttttaggtat
     7801 gagtaatgat attaaacata tcgttgtgta taatccagct ccattagcga tcaaagatgt
     7861 gagcggcctt tatgccgatc aagaagaact taaaaaattg attgaaaagt acgatggtca
     7921 cattgtaaga tttgtgtctg atgaagacga attagatgca ggtgtccgca atcatttata
     7981 tgaaactgct ggagaaaaaa tagtacttaa aaatggagaa ggccatgcaa tgagtggtat
     8041 tttaatgagc agaacacagg ctataatctt agctgaatta aacaaagtta aaggctacca
     8101 agacgaaaat aataaagcat taaaatccgt tcgtaaacaa acgaggcata gattacataa
     8161 agtagagacg ttaagagcga attggattca aacaacgggt ggatcactct cttcctccca
     8221 acaacaatta ttagaagctt taacagcact aaccattgcc gaaggcttaa atcaattagt
     8281 gaatgaagaa agccaacatt tgaaaaaatg tatcacgcga tggcacataa atttggagac
     8341 aactggaaaa aagcgcaaga agttggaaat gaaattggtg aaaaattaac ctctgaagag
     8401 gttatagatg aattaagaaa aggtggcgcg tatgaaagta aacttgaaac agatcccaaa
     8461 agaaaaattg atgataagat aaagaaatta aatgatgttt ataaaaattg taatggctat
     8521 atcgcaaaaa ttaaacagag tatcgaagca attgtttcta atgaccaaat gttagcgagc
     8581 cagattgatg ggatgatgta atgtttacta cgtataaaaa tattaatgaa cttgaaaatg
     8641 cctatgatga agaaagaaaa caattgaatg atgcattcaa tcaaattgat gaattaagac
     8701 atcaaacacg caagaaatgt gaacaaatgt atgatcattt cttatatctc aaacataaaa
     8761 tgaattatag tgaagacgct atgatcagga tgacacgtat tatagaatct ttcgatagag
     8821 aaacgaatca acgtatccga catcacgaaa tgaaattaga agattataaa gatgagttaa
     8881 gaagagaata tctaaaacaa tctgacagaa ttgaaggaga tgaataagcg tgggacttga
     8941 ttttagtggt ttaccagatt tagcagtatt ggaacaaatg aaggaaaaag aacagattag
     9001 tgaggttatt gcgcctgaac atgttcgtat gcatcatgat catcaaaata agctgaaaag
     9061 tgatgagaaa atattacttg accaaatggt aagtcatttc aaaaaatttg aagatgattt
     9121 taaaaatgcg gcacaagggg cttgggtgaa aaatgccaca gacgaattaa aagatattag
     9181 taatgattta gaaaaaattc aagatattaa agtataaaaa ggtattaaga aacacaataa
     9241 gtatataatc catttaataa taaatgaatt atatagttca taatttcgac tataagtggc
     9301 tattagccat tatttttcgg gatctatgtc aaatcggact aatgaattca ataatggaag
     9361 ttaagcaacc aatctttgtt taacttcttt tttatttttt ggaaaataaa gttttgaaca
     9421 taataaaatt tgattatgtt ttaacgaatt ttgatgtttc ttgaactata tatcatctag
     9481 tcgtcattta caattggtaa atatgacttc aaactgtatg aaggtgatgg cgattaaaag
     9541 gctcatccgt aggttctaaa gaactagaat ttaactttat aagaaataaa gaagaaaatg
     9601 tgtatttttc tgatagtatt aatttcaaac ctactgaata gggttaatag aagtttgaaa
     9661 t
//
LOCUS       DALAGF010000103         9465 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.103, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000103 DALAGF010000000
VERSION     DALAGF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9465
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.103"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(67..621)
                     /locus_tag="O2692_002248"
     CDS             complement(67..621)
                     /locus_tag="O2692_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCZ4299858.1"
                     /translation="MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKAEQTTD
                     NELWKNVRDALKEANIIDKTDNENVKVTYKLANGGENSIEGTANLNELSNSNNKPINS
                     DSVKSIDLSRKNPNGDRFDANDAWKKLTDKLKEKQIVKDGDTVTIHSKDEKDPKISAK
                     VGEVYNDNKRLMLFKKDIDKITIN"
     gene            1655..1849
                     /locus_tag="O2692_002249"
     CDS             1655..1849
                     /locus_tag="O2692_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCZ4299859.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(2082..2933)
                     /locus_tag="O2692_002250"
     CDS             complement(2082..2933)
                     /locus_tag="O2692_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCZ4299860.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTSLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
     gene            3261..4022
                     /gene="sufC"
                     /locus_tag="O2692_002251"
     CDS             3261..4022
                     /gene="sufC"
                     /locus_tag="O2692_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCZ4299861.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            4120..5427
                     /gene="sufD"
                     /locus_tag="O2692_002252"
     CDS             4120..5427
                     /gene="sufD"
                     /locus_tag="O2692_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCZ4299862.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            5533..6783
                     /locus_tag="O2692_002253"
     CDS             5533..6783
                     /locus_tag="O2692_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCZ4299863.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            6773..7237
                     /locus_tag="O2692_002254"
     CDS             6773..7237
                     /locus_tag="O2692_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCZ4299864.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            7388..8785
                     /gene="sufB"
                     /locus_tag="O2692_002255"
     CDS             7388..8785
                     /gene="sufB"
                     /locus_tag="O2692_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCZ4299865.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            8972..9109
                     /locus_tag="O2692_002256"
     CDS             8972..9109
                     /locus_tag="O2692_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCZ4299866.1"
                     /translation="MMVDKATTQIQHDCGIRVLVFIKLIESYIKYSLDNSILVRLRFV
                     V"
     gene            complement(9115..9429)
                     /locus_tag="O2692_002257"
     CDS             complement(9115..9429)
                     /locus_tag="O2692_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299867.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLATSVVNLILLIVF
                     LF"
BASE COUNT         3382 a         1369 c         1687 g         3027 t
ORIGIN      
        1 atttgagata ctgcctctta aaaaaagaca agttacatta aaaaagtaac ctgtctttat
       61 ctataactag tttatagtga ttttatctat atccttttta aacaacatca atcgtttgtt
      121 atcattatag acttcaccga ctttagctga gattttaggg tctttttcgt ctttactatg
      181 aatagttact gtatcaccgt ctttcacaat ctgcttttct tttaatttat cagttaattt
      241 tttccatgca tcatttgcgt caaatctatc tccattggga tttttccttg acaaatcaat
      301 gctttttaca ctatcgctat tgatcggttt gttattagaa ttgctaagtt catttaaatt
      361 ggcggtacct tcaatactgt tttcaccgcc atttgctaat ttataagtca ctttgacatt
      421 ttcattatct gttttatcga taatattcgc ttcttttaaa gcgtctctta catttttcca
      481 caattcgtta tctgtcgttt gctcagcttt tgcaacgtta ttaataccat tataatttga
      541 agaagaatga aaacctgaac ctactgttgt taaaactaaa gcacttgcta tcaatgtttt
      601 tgttaatagt tttttattca ttttattttc tcctataatt tatttgcaat cgattacaaa
      661 gtaattttac aattattatt tatgtaaatc aattaaataa ttattaacaa agatataaaa
      721 tattattatt acaatatatt aattttgagc tggaaatatt cgttatttat gctataatca
      781 ttttagacac agcaatgtgt tcaaattttc atctattcgt aagttagcct tcgggctgac
      841 tttttatttc cattattcac atgttaatct tgttgttatt taggcaggta cttcggtact
      901 tgtctatttt tttatgttat aattacatgt gtatatagta ggagtgaact atatagcccg
      961 gcagaggcca tatatctggc tgttggtctc acaggagaca tcttccttgt catcactcat
     1021 atacatatat cttgataaca tagagttgtt atagtcgcta cgccactcat actagttact
     1081 gggtggttgt ttttatgtta tattataaat gatcaaacca caccacctat taatttagga
     1141 gtgtggttgt tttcaagggc aaaaaaaggg cataattttg aaacaaaggg caagggtatg
     1201 aataataaat tatcttgaaa attttataaa tgcatatata acaatgtttt gattgccctt
     1261 gaacttcctt gataatctgg ctaattattc ctgctaaata aatcatatta atggctcagt
     1321 aacgtgatgt tgctgggctt tttaatttaa acaggtattt atatgatatt taggaatgag
     1381 atgctattac ggataaagta aaatcctata tgcatgcgtg ctactcattt attcttattt
     1441 ttaatttcac atggctagtg actaatacat cataaatcat tagtgattct ttattatttc
     1501 agtcccactc cctcaagtta atgatgaatc tatttcgcta gttataaaat gacgtcaaat
     1561 caatatcaaa aattatttaa gtaaaatgtt tagataattt ttcagtgggt aagtattata
     1621 tataacattt aattattccg aggaggcatt tattatggca gacgaaagta aatttgaaca
     1681 agcaaaaggt aatgttaaag aaacagtagg taatgttact gataataaaa atttagaaaa
     1741 cgaaggtaaa gaagataaag cttctggtaa agcgaaagaa ttcgttgaaa atgcaaaaga
     1801 aaaagcaact gattttattg ataaagtaaa aggtaacaaa ggcgagtaat tttacttttt
     1861 attcctaata cactgcacat gccacttagt cggtataacc atcggctagg tggtttttgt
     1921 attaaaaaag tggataccaa aatttattaa taattatttt aatgttagaa aaaaactaaa
     1981 taaaaactcg ctaatgatat ccaataatat gtatacaaaa cgagacatat attgcatatg
     2041 attaacgaga tactgaaaat attttatcac ccctaaaatg attattcatt ttcagcggta
     2101 attcgaccta aagtcaaact tacaataaaa ccgatgataa atactactaa tgaaacgaac
     2161 cacatcacga tattagttgg taaacctgga aatactgcaa agagggagcc aacaacaaaa
     2221 ccaatgatta atgcaaaagt cattagttta tgatgtgtta ggaaatactg gataattttg
     2281 cttgaaataa tgaatccagc aagcacgcca aatccgactg caagtaatat aggaagacct
     2341 gcaaagttaa gtttaacaac ttcagatatt gctagcatga ccgtaccata gacgccaaat
     2401 actaataaca taaatgaccc tgaaatacct gggagtaaca tagcactaga tgcacacata
     2461 cctgcaataa aatatttaat aataagacta gttgatagag taagtgtttc tccagcatgt
     2521 ttatcaccat tattcattaa tgtaataaca attaagatag cgataccagc tataaccatc
     2581 atgtaatgtt tagttgtaaa tgacgtttta tagttagaaa ttttcaataa atatggaacg
     2641 ataccaatga ttaatccacc aaagaaaaac atagttggaa tatggtgttg gcttaataaa
     2701 taattaaaaa gattacttag tgatcccatt gccagtaaca ttccaattat aatggggatt
     2761 aaaaatgtaa aacttggcca aaaacgtcgt gagaatatgc cgctaattga agcgataaat
     2821 tgattgtaaa tacctaacaa taatgcgata gtcccaccgc taacaccagg taccaagtca
     2881 ctcgttccca tagcaaaacc ttttagaata ttaatccatt taaattgttg catgaataac
     2941 tcctttcaaa cgattggaat aaaatcataa atagcatcat accatattac aaatgtccta
     3001 gtgaaatgat aacatatttt aaattcataa aatccattga gaaattatgt gcacttatta
     3061 tcatttatat ttttaaagag agcggattag agttgtactt tagagttatt aaaaataata
     3121 aaaagggttt aagttgttta tattaaggta taagtaagtt ataattaact gaacgcatta
     3181 ttacaaagtc tttttgacta caaattaaaa ttattataaa ctagttaaga aaactttata
     3241 ttttacggag ggaatataaa atggcatcaa cattagaaat caaagaccta catgtgtcta
     3301 ttgaggataa agaaatctta aaaggtgtta acttgacaat taacactgat gaaatacatg
     3361 cgattatggg accaaacggg acaggtaaat caactttatc atctgcaatt atgggacacc
     3421 caagctatga agtaactaaa ggagaagtac ttttagacgg tgtaaatatt ttagaattag
     3481 aagttgatga aagagcaaaa gcaggattat tcttggcaat gcaatatcca tcagaaatta
     3541 caggtgttac aaatgctgat ttcatgcgtt cagcaatcaa tgcgaaacgt gaagaaggac
     3601 aagaaatcaa cttaatgcaa tttattaaga aattagataa aaacatggat tttctagaca
     3661 tagataaaga catggcacaa cgttatttaa atgaaggttt ctcaggtgga gagaagaaac
     3721 gtaacgaaat cttacaatta atgatgttag aacctaagtt tgcaatctta gatgaaatcg
     3781 attcagggtt agacatcgat gcattaaaag ttgtatctaa aggtattaac caaatgcgtg
     3841 gggaaaactt tggtgcatta atgattacac actatcaacg attattaaat tacattactc
     3901 ctgataaagt acatgtaatg tatgctggta aagtcgttaa atctggtggt ccagaattag
     3961 caaaacgtct tgaagaagaa ggatatgaat gggttaaaga agagttcggt tcagctgaat
     4021 aatcttatta atacagtatc catgagatgt tcatctatat atgatgaaaa tgaacattta
     4081 tacgaaatag taaatttcat caagtaggag gaaaaagtta tgacaactga tattttgaac
     4141 atttctgaag aacaacttgt tgattattct aaagcccaca atgaaccttc ttggatgaca
     4201 gaattacgta aaaaagcttt gaaattaaca gaaactttag aaatgccaaa acctgataaa
     4261 acaaaattaa gaaaatggga ttttgattct tttaaacaac acgatgtaaa aggtgatgtt
     4321 tatcaatctt tatcacaatt acctgagtca gtaagagaaa ttattgacgt agatcattct
     4381 aaaaacttag taattcaaca taataatacg attgcgtaca cacaagttga tgataatgca
     4441 tcgaaagatg gcgttatcgt tgaaggttta gcagacgctc ttatgaacca tagtgattta
     4501 gtacaaaagt actttatgaa agatgcagta acagtagatg aacatcgtat cacagcgcta
     4561 cacacggcat tagttaatgg tggcgtattt gtttatgttc ctaaaaatgt agttgtagaa
     4621 catccagtac aatacgttgt gttgcacgac gacgaaaatg caagctttta taaccatgtt
     4681 atcatcgtta ctgaagaaag cgccgaagtc acatatgttg aaaattactt atcaaatgca
     4741 tctggtgaag gaaatcaatt aaatattatt tctgaagtga ttgctggtgc aaattcaaat
     4801 atcacatatg gctcagtgga ctatatggat aaaggcttta caggtcatat cattcgacgt
     4861 ggtattactg aagcggatgc ctcaattaat tgggcactag gtttaatgaa tgagggtagc
     4921 caaattattg ataatacaac aaatttattt ggtgatcgtt caacaagttc acttaaatca
     4981 gtagttgtag gtacaggcga acaaaaaatt aatctaacat ctaaaatcgt acaatatggt
     5041 aaagaaacag atggttatat ccttaaacat ggtgttatga aagaacatgc atcgtctgta
     5101 tttaatggta tcggctacat taagcatggt ggaactaaat caattgctaa tcaggaatca
     5161 cgtgtattaa tgttatctga acatgctcgt ggtgacgcga atcctatttt attaattgat
     5221 gaagatgatg tacaagctgg tcatgctgca tcagtaggtc gtgttgatcc agatcaactt
     5281 tactatttaa tgagtcgtgg tatttctcaa agagaagcgg aacgtcttgt tatacatggt
     5341 ttcttagatc cagtagtacg tgaattacct atcgaagacg ttaaacgtca attgagagaa
     5401 gtaattgaac gcaaagtttc taaataatat tttgaaaata aaagtttgta atagatatag
     5461 actgtcgata ttggtataag actaatacaa cgtcagtatt taaatgatta ggatttttat
     5521 ttaagaaagg tcgtgaatga agtggccgaa cactcatttg acgttaatga agtaatcaag
     5581 gattttccga tattagatca aaaagtcaat ggcaaacgtt tagcatatct tgattcaaca
     5641 gcgacaagtc aaacgcctgt gcaagtgtta aatgttttag aagattacta caagcgttat
     5701 aattcaaacg ttcatcgtgg tgttcataca ttaggatcat tggcaactga tggttatgaa
     5761 aatgcccgtg aaaccgttcg tcgttttatt aatgcgaagt attttgaaga aatcattttt
     5821 acacgcggaa caactgcgtc gattaacctt gtagcacata gctatggtga tgcaaatgtt
     5881 gaagagggcg atgaaattgt tgtcactgaa atggaacatc atgccaatat tgttccttgg
     5941 caacagttag caaagcgtaa aaatgcgaca ttgaaattta taccaatgac agctgacggt
     6001 gaattaaaca tcgaggatat taagcaaacg attaatgata aaacaaagat cgttgctatt
     6061 gcacatatat ctaatgtgct cggtacaatt aatgatgtta aaaccattgc agaaatagct
     6121 catcaacatg gtgcaattat cagtgttgat ggggcgcaag cagcaccaca tatgaaactt
     6181 gatatgcaag aaatgaatgc tgatttttat agttttagtg gtcataaaat gcttggacca
     6241 acaggtattg gcgtattatt tggtaaacgt gagttactac aaaaaatgga accgattgag
     6301 ttcggtggcg acatgattga ttttgtaagt aagtatgatg caacatgggc tgatttacct
     6361 actaaatttg aggcgggtac tccattaatt gctcaagcaa ttgggcttgc agaagctatt
     6421 cgctatttag aacgcatagg ttttgatgca attcataaat atgaacaaga attaacgata
     6481 tatgcttatg agcaaatgtc tgcaattgaa ggaattgaaa tttatggccc gccaaaggat
     6541 cgtcgtgcag gtgtaataac gtttaattta caagatgtac atccacacga tgttgctaca
     6601 gccgtagata cagaaggtgt agcggttaga gctgggcatc attgtgcgca accgttaatg
     6661 aaatggttaa atgtgtcttc aacagctaga gcgagttttt atatatacaa cacgaaagaa
     6721 gacgttgatc agttaataaa tgccttgaaa caaacgaagg agtttttctc ttatgaattt
     6781 taataatcta gatcaattat atagatctgt cattatggat cattataaaa atcctagaaa
     6841 taaaggtgta ttagataacg ggtctatgac agtagatatg aataacccga catgcggtga
     6901 ccgtatacga ctaacatttg atatagaaga cggcattata aaagatgcta agtttgaagg
     6961 tgaaggttgt tcgatttcaa tggcaagtgc atcgatgatg acacaagctg ttaaaggtca
     7021 ttcacttgga gaagcaatgc aaatgagcca agaatttacg aaaatgatgc ttggtgaaga
     7081 ctatgtgatt acagaagaaa tgggagatat tgaagcattg caaggtgtat ctcaattccc
     7141 agctcgtatt aaatgtgcca cattagcttg gaaagcattg gaaaaaggta ctgttgctaa
     7201 agaaggtaaa gcagaaggta cgactgaaga agaatagaat gctgttaatc atagataatt
     7261 ttgatattag acatataaaa gtataaaaat ttttataaga tgtcatgtca ttgttataat
     7321 atggtttaca tcatgaatta aaaacttacg cacgccgttg taaatatatt tttaaggagt
     7381 gattgaaatg gctaaaaaag cacctgatgt tggggattat aaatatggat tccacgacga
     7441 tgatgtgtcc attttcagat cagaacgtgg tttaactgag aatatcgtta gagaaatttc
     7501 taacatgaaa aatgagccgg aatggatgtt agatttccgt cttaaatcat taaaattgtt
     7561 ttataaaatg ccaatgcctc aatggggtgg cgacttatca gaattgaatt tcgatgacat
     7621 tacttactat gtaaagcctt cagaacaagc tgaacgttca tgggatgaag tgccagaaga
     7681 aattaaaaga actttcgata aattaggaat tcctgaagct gaacaaaaat atttagctgg
     7741 tgtttctgct caatatgaat ctgaagttgt ttaccataat atggaaaaag aacttgaaga
     7801 aaaaggtatt atctttaaag atacagatag tgctttacaa gaaaatgaag aattattcaa
     7861 aaaatacttt gcttctgtag tacctgcagc agataacaaa tttgcggcgt taaactcagc
     7921 agtatggtca ggtggttcgt tcatttatgt acctaaaaat atcaaactag atacgccact
     7981 acaagcttat ttccgtatta actctgagaa catgggtcaa tttgaacgta cattaatcat
     8041 tgctgatgaa ggtgcttctg tacattacgt agaaggttgt actgcaccag tttatacaac
     8101 tagttcttta cactctgctg ttgtggaaat cattgtgcat aaagatgcgc acgttcgtta
     8161 tactacgatt caaaactggg cgaacaatgt atacaattta gttacaaaac gtacttttgt
     8221 ttatgaaaac ggaaatatgg aatgggtaga tggtaactta ggttctaagt taacgatgaa
     8281 atatccaaac tgtgttcttt taggtgaagg tgcaaaaggt agtacattat ctattgcatt
     8341 tgctggtaaa ggacaagttc aagatgccgg tgctaaaatg attcataaag cacctaatac
     8401 atcttctaca attgtttcta aatctatttc taaaaatggt ggtaaagtta tttatcgcgg
     8461 tattgttcat tttggacgta aagcaaaagg tgctcgttca aatattgaat gtgatacatt
     8521 aatcttagat aacgaatcaa catcagatac aattccatat aacgaagtat tcaacgatca
     8581 aatatcatta gaacatgaag ccaaggtttc aaaagtttct gaagaacaat tattctatct
     8641 aatgagtcgt ggtatttctg aagaagaagc gacagaaatg attgttatgg gattcatcga
     8701 accatttaca aaagaacttc caatggaata cgcggtcgaa atgaaccgtt taatcaagtt
     8761 cgaaatggaa ggtagtattg gatagcttta aaccgcgttg ttaagccatt cttgacttcc
     8821 gaaaatggct attgatacca ttttgatact gaatataaca aaaagccaca ttactgtggc
     8881 tttttttgtt ttataactaa atcggattga tagataagct ttgtacttat ttatatcagt
     8941 tcgatttttt gattggtgta aaaaataatc attgatggtg gataaagcga caacacaaat
     9001 acaacatgat tgtggcatta gagtgctggt ctttattaaa ttaattgaaa gctacatcaa
     9061 atattcttta gataattcga tattagttcg attaagattc gttgtataag tgagttaaaa
     9121 taagaaaact attaataata ttaagttcac tacagatgtt gctaatggac cataagtttt
     9181 aaagacatct tcacttttat aaccaacaat cgcatctaaa aattgaacta agatcattgc
     9241 aatggatata gttatcaaaa atatagcact atgaatgact aaagaaaaaa tagctaataa
     9301 aaataaaggt aagcttcgac taagtgcata atatgcattt atattatggc tagatgcaca
     9361 tgcttgaatt gaataaccta aacttacact ggcactgatt attgtaaata ttgctaaaac
     9421 aaaatacatg ttaatccttc tttctatatt tggatataaa caagt
//
LOCUS       DALAGF010000104         9420 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.104, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000104 DALAGF010000000
VERSION     DALAGF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9420
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.104"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(237..743)
                     /locus_tag="O2692_002258"
     CDS             complement(237..743)
                     /locus_tag="O2692_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299868.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            complement(879..1679)
                     /locus_tag="O2692_002259"
     CDS             complement(879..1679)
                     /locus_tag="O2692_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4299869.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLTLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTAAFINKHHDEKHV"
     gene            complement(1663..2382)
                     /locus_tag="O2692_002260"
     CDS             complement(1663..2382)
                     /locus_tag="O2692_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ4299870.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            complement(2437..3387)
                     /locus_tag="O2692_002261"
     CDS             complement(2437..3387)
                     /locus_tag="O2692_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCZ4299871.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVTGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            complement(3535..4422)
                     /locus_tag="O2692_002262"
     CDS             complement(3535..4422)
                     /locus_tag="O2692_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ4299872.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSNDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            complement(4801..5436)
                     /locus_tag="O2692_002263"
     CDS             complement(4801..5436)
                     /locus_tag="O2692_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HCZ4299873.1"
                     /translation="MLGTDELYKVLYEHLGPQFWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            complement(5824..7485)
                     /locus_tag="O2692_002264"
     CDS             complement(5824..7485)
                     /locus_tag="O2692_002264"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCZ4299874.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(7482..7898)
                     /locus_tag="O2692_002265"
     CDS             complement(7482..7898)
                     /locus_tag="O2692_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCZ4299875.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(7991..8098)
                     /locus_tag="O2692_002266"
     CDS             complement(7991..8098)
                     /locus_tag="O2692_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299876.1"
                     /translation="MKDSENLVFFGFEVLFVFYKMAYIYEALIKYGIVN"
     gene            complement(8173..9183)
                     /gene="adhP"
                     /locus_tag="O2692_002267"
     CDS             complement(8173..9183)
                     /gene="adhP"
                     /locus_tag="O2692_002267"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCZ4299877.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
BASE COUNT         2938 a         1813 c         1218 g         3451 t
ORIGIN      
        1 caaatgcggt gataaacctt tatgattaaa gtaatcttct atattttcta gtaaaatttt
       61 atcgttaata cgcattggta gtactccttt aaaatgaaac atctttattt attgtattgg
      121 tttaatttcc ccttcgcaaa tcataaaaat taatattcac tgtattatgc catgtaataa
      181 aataaccgac aagtattgcg atttaacttg tcggttaagc acgattaatt taattattat
      241 ttttccaaat caatacgata taattttatg tttttatctt ttggtgctga ggcaaattga
      301 taagtcgttt tgcctttttc aatatatcca aaattaccag caactttgct atattccgta
      361 tgtgttactg ctttgccaac ataattttta acagcttgat atgttggacc attagcatca
      421 ttatacatca ttgaaatatg agttacttta ccattgtttt tcttaccatc agtagttact
      481 aataacatac cttttttagt atggaattcg taataatgtt cagttccatc ttcattgtaa
      541 gaatacatcg gatttttgta cttagttaaa acatctttaa tagattcgcc aattttcaca
      601 ttttctaatg actgatcacc cttaattaat tgtttaacag tatcaataga gttgccactt
      661 gctgcttcgg cactagtgtt cactaagcct actcccatta caacagaaca tgcaattata
      721 cttgccgtta gtagcttttt cataagcact ctctcctttt atttatatcg tcttgtgctc
      781 acaaccatta tacaacaatc atttctaaaa caacagtcat attacaatta tattacaaat
      841 aataattact acttttatat ttcacttatc actaaaaatt aaacatgctt ttcatcgtga
      901 tgtttgttga taaatgcagc tgtatcttcg acgaatcttt cttgctcttc aacaaatggg
      961 aatgcacttg attcttgata tacttcaaag tccgcatttt ggattaaatc agctacttct
     1021 ttagcttcta atcttgttgt tctttcaccg aattctcctg caataatttt tgtcggtact
     1081 acgacatttc tataagtttg agaaatatca gcgtttttga atacctcttt aactgcttga
     1141 atttcttctt tagttgatat ttcatttgta tctacgacat gtttgaggaa tcgattcatt
     1201 tttctcggac gataatactt acgcttattt aaaaatttat cttgtttttc aggatcccag
     1261 tttcgaataa tatgggcata ttttctaaat aaacgttctt ccggtaattc accttcaata
     1321 gatgttggat ttaccaacgt aagtgatgat gtaaattcag gataacgtac tgaaatatcc
     1381 gcaccaatga ttccacccat ttcatggcat acaaatgcaa cttcttcaat atataaatat
     1441 ttaagtaatt caacaatgtc atcagaaaaa tcttttattt caatgtgacg aggtttatca
     1501 gaatagccat gtccacgtaa atcaattaac acaacttgaa atgattttgc taattctgct
     1561 gctaatttat taaacacaga ataattatca agtacagtat gaatcaatac gataggatag
     1621 ccttcaccta atgtactgta atgtatcgat gttccatctt ttctagtaaa tagatccata
     1681 attttctcct ttattatcat tttttattgg taaaatggtt aataattctt ccactgttgt
     1741 aattgtaaaa tcaacttctt cttcgagtgg ttcaatttca gcatcttctt gtttatacca
     1801 tacactaacc atgcccatag ctcgtgctgg agctacatca tttaacgcat catcgccaac
     1861 atacataatt tgctcaggta atacccctag ttgatcaatc atatcttcaa aaattttagg
     1921 atgtggttta cgaaaaccaa ctgtttctga tgttgataaa taattaataa catgcatcaa
     1981 accaagtgaa tgtaatcgaa attgtttaat cttagattta ccatttgcga taacaccaat
     2041 ttgaaagcct ttttgcgata gcttttctag tgtatacaaa gtatcgtaat aaggaaatac
     2101 ataacgataa aaatgcattt caaaatcatt aaataagtct ttccaagtta acctatctac
     2161 atggaaacgt ttaatgattt ctttatataa atcaggttta tcattatctt catcgtcatc
     2221 tagctcaata aatgcttttt taaaatctgc cagttgaaca tgaattaagt agtcatgaaa
     2281 tcgttcatat tgctcttcga taaatttctc tcgagattta actctgtcca acaacgtacc
     2341 ttctaaatca aatacaactg ctttaatttg attcaaatcc atcgcttatg ccctctttat
     2401 gtttatagtt atttttattt tagcaaatgt cgtgtgctag tgtaaccgtt tcgactgaga
     2461 tattagcatg taaaataaca ctggtatacc aattaatgca atgataatac ttgccggtat
     2521 ttgtactggt gacaatatat ggctaccaat aaaatctgcc ataaccatta tttgtcctcc
     2581 agttacaata tttaaagcca attgtcttcc taatgattgt ttccactgcc attttctaat
     2641 gagttgcggt atcaccatac caatgaatcc aatgatgcca acatatgcga catttatcgc
     2701 cgttatcata gaagctatac ataacgcgat atatgtaatc aattgaacat ttaagcctag
     2761 tgactgactt tttagttctc ctaattgcaa taactttagt tgattaagaa tgataaatat
     2821 accaaacaat gcaatgataa atgtgattgc tattatagat acatttgagt attctgcatc
     2881 accaaaacca ccaaacagat aattggcaat tgtatttaat ttgcgaggtt ttaataaaat
     2941 caaaaaatat agaagtgaat tgaataacgc accaatcatt aaaccactta atattaagat
     3001 tctaactgga tagccttggc tcaataccga cgtaatgact aatacagtta ttaatgttat
     3061 caaactaaat gttattgaaa atacaggaat ccataacgtt gttaaaccta aaaataatgc
     3121 taatcctgaa ccaaatgtag cgccgcttgc caatcctaat gtaaagctat ctgccaatgc
     3181 attatttaaa actgtttgaa atataaggcc tgcaacagtt aatatcatac ctgtcaacaa
     3241 tgcttctaat aatctcggta atctaacatt gattaaaata gactggttaa atacatcatt
     3301 gatatcgccc aactgccaaa atagatatat gctagttgtt ataatcaata tgactatcca
     3361 gctcaataat actttattaa atgtcatagc attctctttt ctttcatcga taattacatt
     3421 cattatagca atcacatact cgcgttgcta tcattcacta aatatatttt aaaaacccct
     3481 tatctcaaac atgtattttt tacatgtagc aataaggggc ataattagaa tggtttattt
     3541 tctataaatt gcatctctta attcttttaa tccttcatca atacgtggac ctggtcttga
     3601 tacttcatca ccatttacaa cttcaatacg tgtattcttg acagcattaa ttttattgaa
     3661 tccacctctt tttttgatga tatccatata atctgatctt gtcttagctt ccgtcgaaat
     3721 taatatatct gggttctttt taataatact ttccttcgtt acagggttcc aaccattaat
     3781 gtcactatac acattttggg cttctaattt ttctaacata tcattaaaaa atgtatgctt
     3841 ccctgctgta tatatttcag gctttgatga aacctcaata aatacttttg attttttatg
     3901 atgagcagga attgaatcta tgactttatc gatattatct ttagtttcct caacaagttg
     3961 ttcagcctgc ttatcatgat gcgttaattt cccaatttgc ttaaatgtgt tgtaagtttc
     4021 atcaattgat tgtgcatctt taacatacac tactttgatg ccttgtttct ctaatgatga
     4081 caatacttta ttagcagttg ccttttgcga ctcatgcgca agaattagat ctggctttgc
     4141 ctttaaaagt tcctctttat ttagattcaa agcatcaaat tgtttcttac cctttttcac
     4201 atcttttgga taatcatcaa ccgttgaaac accaactatg tatttaccta atcctaattc
     4261 atataaaatt tcagtattac taggcattaa cgaaacaatt ctatggtacg gcgatttaga
     4321 cgagccatta tcattgctac cttgtttatc attatctttc ataccacagc cactcaggac
     4381 aagcataaaa atcaaaataa aagcaattaa cgatttcttc acactatcac atctcctttt
     4441 taatcccact tttcgcttta gcgattagag ggatttatgc agttgtgcgg tagcatccaa
     4501 ctgcgttcct cttcatcttt agcgattaga ggcttttatg cagttgtgcc ttagcatcca
     4561 actgcgttcc tcttcacctt tagtgattag aggcttttat gcagttgctg cacaaacaac
     4621 tgtattcctt tactcccctt gagctttagc gattaggggc tcttatgcag ttgctgcaca
     4681 aacaaacaac tgtttttttc tttatatgct tctatttatt atataagtcc acatactaaa
     4741 agaaaatagt aaattatttt ttatatttca tataaactca tctatctaac ttcacagcgt
     4801 ttacttttta aagtaaggtt ctaaaaaatc ataattcttt atatctttgt ctctaaagta
     4861 atgtttacca aatacatcta acagagcatg aaattcatta gcatcttgat ttgtaaaatg
     4921 atttggtaat gtgactactt tttttaattg atcataattt ttagtgtttt catatcctaa
     4981 tttatcatat atttttcttg tatagctatc tggaataaat tcaatacgtc cgaatatata
     5041 aacaagtaag acatctgctg tttcacttcc aatacctttc aaagataata attcttttct
     5101 taatccacct ttatatcgct cattaatctc ttgataattg aaatgatgtc gtgctaacca
     5161 tgttaataat gttttaatcg tcagtgattt acttttataa aagccacttg aatgtatcaa
     5221 tgattgtaac gtttcaatag gtagttctaa tatatgattt ggattaaaat gcgtatgttc
     5281 tttaatctga ttcaatgcaa tttctgcatt tcgccatcta gtattttgaa ctaaaattgc
     5341 acctaacatc atttcaatgt cattatcagc aggccaccaa aattgtggtc cgagatgttc
     5401 atataaaact ttatataatt catcagttcc taacatttac accctccatt ttagagggag
     5461 tgggacagaa atgatattat cgtaaaattt atttcgtcgt cccaccccaa cttgcattgt
     5521 ctgtagaaat tgggaatcca atttctcttt gttggggccc cgccggcaag gttgactaga
     5581 attgaaaaaa gcttgttaca agcgaatttt cgttcagtca actactgcca ttataacttt
     5641 gtagagcata gaacattgat ttatgtctca ggctctctta tgcagttgtg cagagcatcc
     5701 aactgtgttc ctcttaacct tattcacata ctaagcattc cccaatatgt cacataccta
     5761 aaaaaacact ccaacacaaa gtatctttcg agaaactcta cgttggagtg tatataaatg
     5821 ttcttacatt gattcaggtg cgcttacacc gaccattgcc aatgcatttt tcaatgtaat
     5881 tctgaccgct tcaatcatag caacatgtgc ttttgttttt tcaatatcat ctgttaacac
     5941 tttttcagca ttatagaatt tatggaaatg agaagctaaa tcttgaatat aattagtaat
     6001 tctatgtgcc gatctatgct cagcagcact ttcaattgta ggttcgaaat cagctacttt
     6061 tttcaacaat tcaatcgctt tttcattagt aatcgttgta aaatcattcg cagcagtcac
     6121 ttcaatacct tgctctttcg cttgttttaa aattgaacaa atacgcgcat gtgcatattg
     6181 agcatagtaa actggattgt cttgagattg ctctttcgct aattccatat caaaatcaaa
     6241 gtgactatca ggactacgca tagttaagaa ataacgtgca gcgtcaacgc caacttcgtc
     6301 cataatttct cttaatgtaa tcgcattacc agtacgttta ctcatcttca cttctttacc
     6361 attttccatt aaacgaacca tttgcatgat ttgaatttct aaacgattac tatctacacc
     6421 aaacgtttca agagatgctt tcaaacgatt aatataacca tgatgatcag caccaaataa
     6481 atcgattaaa atgtcattac cacgttttac tttatcgaag tggtacgcaa tatctggtaa
     6541 gaaatacgta tatgtaccgt catttttaat taatactctg tctttgtcgt ctttaaaatc
     6601 agttgtacgt aaccatgtag cgccatcagc ttcatacgta taacctaatt ctttcatttt
     6661 tgctaaaact tcaagaattt ctcctttttc atataaagat gtttcactaa accaattatc
     6721 aaaatgcgta ttgaactctg ctaaatcatt tttcaattta gccatttcgt attctacgcc
     6781 taattttcta aattctttca aacgtgcttc ttcagaataa tctttaattt cagggtgttt
     6841 ctctgctaaa tctttaccta tttcaataat atcttttcca ttatagccat cttctggcat
     6901 tgaataacta ttgtcaccta aagcttcaaa gaaacgtgtt tcaatcgaac gcgctaagtt
     6961 agtaatttga ttaccagcat cattaatata atattcacgt gttacattat agccagctgc
     7021 agttaaaata ttagctaaag catcaccaac tgctgcattt ctagcatgac caatatgtaa
     7081 atctcctgta gggttagctg aaacatactc aagcaataca ttttgacctt ttgattcatt
     7141 tacatgtcca aattgatcac ctttttcaat tgcttcagga ataattgctg ttaaatactg
     7201 attatctaag taaaaattaa tgaatcctgg accagcaatg tcaatttgtt ttacatgtgc
     7261 tttttcagta tctaagttat caacaatcgc ttgagcaatt tcacgaggat tacgctttgc
     7321 aatcttagtc agtaccatcg caatattagt agcataatct ccattttttg tatctttagg
     7381 aacttcaatt ttaatatcag gaatctcatc tgctaatcct gctttgttaa tacttgctgc
     7441 aatttcttct actaatgttt gtttcacttg atcaataata ttcattcgcc tatctcctta
     7501 tagttaattt catattgata agaacccatt ttttcattat cttgatataa ttcataatga
     7561 atctttagct tgccaccgtc tccacttacg aaatgtaaaa tgcgtaatgt tttaacttct
     7621 agtggaattc gtccagccga tatatcgtaa aaagttgttg tcgtttgtcc ttcaacaaaa
     7681 tgcaaattca tattaatgtc gcctttacga atcaacttaa cgctatcatc atccactttt
     7741 attgtaacat taactgttgc atcctcaatt tgttctacat accgaataaa gttagattgc
     7801 ctttgttgcc aagttccttc agtagtaaat tcaaattttt ctttttcgtt gtgctgtttc
     7861 aacacttgct ttgtttgaat acttactttt ttatccaatt tactactcac cttaatcttc
     7921 gcataataga aatattatag cataaaataa ggtcatcttg ttatgccttc ttaaagctaa
     7981 ataggttcaa ttaattaaca attccatact taatcaacgc ttcatatatg taagccattt
     8041 tataaaatac aaacaacacc tcaaaaccaa aaaacacgag attttcacta tctttcatga
     8101 aaacctcgtg tttgattata atgatagtgt tcagagcact accttcttta tctatatagt
     8161 cagttgatat ttttaaaatt taataaccat tctaccagtt atagtaccat tttccatttc
     8221 ttcaaaaata tcattgattt cttctaattt tcttaattga actttaggtg ttactttatt
     8281 ttcagcagca aattcaaacg cttcacgtaa gtcttgtctt gtaccaacaa gtgaacctac
     8341 tacttcaata ccatcaagca ctaatcttgg gatatctaag ttcattttat caacaggtaa
     8401 accaacggca acaactcttg caccagcttt tacaacatca accgcttggt taaatggcgt
     8461 cttagcaact gaagttacca ctgttgcatc taatcctttg ttatcagtta atttcataac
     8521 ttctgcaact ggatcaacat ctttagaatt aataatagca tcagcaccta attctttcgc
     8581 gaatgctaat ttatcatcat tgatgtcgaa ggcaacaatt ttagccccca taacgttttt
     8641 agcatattgt aaagctaggt tacctaaacc acctatacca aaaacaccta accattgtcc
     8701 aggttttaca ttacttactt ttacagcttt ataagttgtc acacctgcgc atgtaataga
     8761 agacgctgct gctggatcta atttttcagg tactttcaca gcatagtctg cagtaacaat
     8821 aacttgttca gccattgcac catctactgt ataaccagca tttttcacac tacggcaaag
     8881 tgtttcacga cctgttgtac aatattcaca tcttccacag ctttcgaaca tccaagcgat
     8941 agacacacgg tctccaattt ttaatgattc tacatcttcc gcaacttcga tgactttacc
     9001 aataccttca tgacctaaag taacgcctgt aacatcacca aaatcagcat tcttaacatg
     9061 taaatcggta tgacaaacgc cacaatattc cgtttgtacc aacgcttcac caggttttaa
     9121 agctcttaac tttttgtcct caatacttac tttgtgatct ttcgttacaa ctgctgctct
     9181 cataataatg tcctccttat agtcactcca gtatgtatga tattaattgg ctattacttt
     9241 caaaaaagaa aacgaataat tatagatgta atttttattt gtaatagacg ccagattaaa
     9301 acatactact gtgaccttgt ttttagttag atatcaaccg ttaactttta acgtttccct
     9361 agttaatatg ttaaaagaaa tgacataatc gatatgctaa cgtgtaattt ttacttcaca
//
LOCUS       DALAGF010000105         9275 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.105, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000105 DALAGF010000000
VERSION     DALAGF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9275
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.105"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            67..528
                     /gene="tsaE"
                     /locus_tag="O2692_002268"
     CDS             67..528
                     /gene="tsaE"
                     /locus_tag="O2692_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCZ4299878.1"
                     /translation="MIKINNLDEMNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            509..1171
                     /gene="tsaB"
                     /locus_tag="O2692_002269"
     CDS             509..1171
                     /gene="tsaB"
                     /locus_tag="O2692_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCZ4299879.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            1144..1608
                     /gene="rimI"
                     /locus_tag="O2692_002270"
     CDS             1144..1608
                     /gene="rimI"
                     /locus_tag="O2692_002270"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCZ4299880.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            1601..2626
                     /gene="tsaD"
                     /locus_tag="O2692_002271"
     CDS             1601..2626
                     /gene="tsaD"
                     /locus_tag="O2692_002271"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCZ4299881.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITATINEALGDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(2728..4338)
                     /locus_tag="O2692_002272"
     CDS             complement(2728..4338)
                     /locus_tag="O2692_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HCZ4299882.1"
                     /translation="MSTNQTFLIFVIAIILLTSVIGIVGRYMSRQRLLKSMETLWQTI
                     SPLEAFIRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNIL
                     FLCPFLPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLK
                     QGYTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLF
                     HSIQKSYTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELT
                     HPLIADAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPG
                     IVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAAS
                     ESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKAN
                     TRNAIELLKITSFPAKIYERAKDNVPKI"
     gene            4427..4555
                     /locus_tag="O2692_002273"
     CDS             4427..4555
                     /locus_tag="O2692_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299883.1"
                     /translation="MIKTMSIENMRTYQQRVNNLWIKGVDNVIYCGYQVGKQKIND"
     gene            complement(4699..6627)
                     /gene="abc-f"
                     /locus_tag="O2692_002274"
     CDS             complement(4699..6627)
                     /gene="abc-f"
                     /locus_tag="O2692_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HCZ4299884.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDCLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLYGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPMSIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLLLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHKKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
     gene            6880..7515
                     /locus_tag="O2692_002275"
     CDS             6880..7515
                     /locus_tag="O2692_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCZ4299885.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            7819..8898
                     /locus_tag="O2692_002276"
     CDS             7819..8898
                     /locus_tag="O2692_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HCZ4299886.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVHQQSAKVKQQTQIV"
     gene            8958..9182
                     /locus_tag="O2692_002277"
     CDS             8958..9182
                     /locus_tag="O2692_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCZ4299887.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
BASE COUNT         3081 a         1340 c         1663 g         3191 t
ORIGIN      
        1 ttagatacga ttgaacaatg gataatcatt tttatgaatg aaaagcataa tataggagaa
       61 tcaacattga taaagataaa taatttagat gaaatgaatc aatttgctat atttttagtt
      121 gagcaattga aaagtggtga tttgatttta cttaacggag atttaggagc aggtaaaaca
      181 acgttaacgc aatttatagg aaaagctctt ggtgtaagac gtacgattaa ttccccgaca
      241 tttaacatca ttaaatcata taggggtaaa aatttaaaat tgcatcatat ggattgttat
      301 cgcttagaag attctgatga agatttagga tttgatgaat ttttcgaaga tcaggcaatt
      361 actgttattg aatggagtca atttataaaa gatttacttc cagcgacgca tttatctatt
      421 aacatttcaa caatatctga aaatacaaga caaattgagt tgttcgcgca aggagaacat
      481 tatgaacaaa ttaaggaggc aattatccat gaattcgctg ctcattgata catcgaatca
      541 accattatca gtagcattga tgcaaaacga tgaagtttta gctgaaataa ctacggattc
      601 taaacaaaat cattcagtgc aattaatgcc tgcgattagt cagctatttg agcagagcaa
      661 aatagctaaa cagcaattag atgcgattat tgttgctgaa ggtcctggtt catatactgg
      721 cttacgtatc ggtgttactg ttgctaaaac attagcgtat gcgttagatg ttaaattata
      781 tggtgtttca tcattaaagg cattggcagc aacaatcgat catactgata aattattggt
      841 tcctgtgttt gatgcaagac gacaagcagt ttatacaggc atattccagt ggcaaaatgg
      901 tcaattagtg actattttag aagatcaata tatgtcgata gaggcattgc gagctttttt
      961 agaagattta aaccaaccat ttatttatat tgggaaagat actgtaaaat tacaagatga
     1021 attacaaggt gaagtgattg cgcagctacc aaatgcatca gtcatgtatc aattgctaga
     1081 taaaccttca gacgtacata catttactcc taaataccat aaattagctg aggcggaacg
     1141 aaattggatc aacagtcaaa agaacaatta aacattagag agatgactaa agaagatgtg
     1201 ccacaagtct ttgatcttga gcgtcggagt ttcaatgaca gttcttggac aattgacgca
     1261 ttttatcatg aaatagagca aaataacttt gcgaaatatt ttgtcttaga atttgaacaa
     1321 caaattattg gttatttagg tttatggatt gttatcgatc aagctcaaat tacaacagtt
     1381 gcaatcgatg ataattatag aggctatggt ttaggtcaaa tgcttttaaa atacggaata
     1441 aactatgcaa gtcacacttg tgatgtgatg agtttagaag taagagttaa taataaagtg
     1501 gcacaacatg tatatgaaaa tttaggattt caatatggtg gtaaacgtaa aaattattat
     1561 ggtgaaggtg aggacgcaat ggttatgtgg gtgaatttaa atgactaaag atatattaat
     1621 actagctgtt gaaacaagtt gtgatgaaac aagcgttagt gttataaaaa atggcagaga
     1681 tattttatca aatacagttt taagtcagat tgaaagtcat aaacgatttg gcggtgtcgt
     1741 tcccgaagtg gcaagtagac atcacgttga aggtataaca gcaacaataa acgaggctct
     1801 aggggatgcc gatgtatcaa tagaagatat tgatgccata gcggttacag aaggccctgg
     1861 actaattggt gcgttactaa taggtgttaa tgcagccaaa gcattggcat ttgcttacga
     1921 taagccactt attcctgttc atcatattgc aggacatata tatgctaatc acatagaaga
     1981 gccattaaca ttcccgctaa ttgcacttat tgtttcaggt ggacatactg aattagttta
     2041 tatgaaggat catttatcat ttgaagtcat tggtgaaaca cgagatgacg cagtaggtga
     2101 ggcttatgat aaagtggcac gaacaattgg tttaaattat ccaggtggtc cacaagttga
     2161 tcggttggct gctgaaggtg aagatactta ttcattccct cgtgtttggt tggataaaga
     2221 tagttatgat tttagtttta gtgggttgaa aagtgccgta atcaatcaac ttcacaatca
     2281 acgacaaaaa aatattccaa tcattgaagc taacgtagca acgagctttc aaaacagtgt
     2341 tgtagaggtg ctcacgttta aagctattca agcttgtaaa gaatatggtg ttcagcgatt
     2401 aattgttgct ggtggcgtgg cgagtaataa aggattacgt caatctttag cggatcaatg
     2461 caaagtcaat gacattcaat taactatccc aagtcctaaa ttatgcacag ataatgctgc
     2521 aatgataggc gttgccggcc actatttgta tcagcaaggt cgatttgctg atttagcatt
     2581 aaatgggcac agcaatatag atttagaaga gtattctgca gaataaaata aacgtcagtt
     2641 attaacatga ttactatgaa cattattgaa actgttcata gtcttctgtc atgtgatagc
     2701 tgacgttttt atgtttaaag ttaaatgcta aattttcggg acattatctt ttgctcgttc
     2761 atatattttt gctggaaatg aagtgatttt taataattcg atggcattac gtgtatttgc
     2821 tttgccaggc ttaattttgt aatcaaaatg tatgttatta ttttctatta cctcattgaa
     2881 atggtaattt tcataacgtt gttttaagag ttcagctaac tcaatatcat gtgttgctgc
     2941 aataactcta aagttagatt tttcatgtaa aaatgatagt actgattctg aagcggcaat
     3001 tcgttcagtt gtgttggtac ctttaaaaat ttcatctata aagcagtata ttttttgatt
     3061 atctggaatt tcaacaatac gcttaataga ttttaattca gccataaaat aactatcgcc
     3121 tgataacaca tcatcggcat ttgccattga cgtgaacaca attccaggtt gatatacaaa
     3181 tttgcttgcg gtaactgttt gtatagccga tgcaagaata atgtttattg ctatggattt
     3241 cataaatgtt gatttacccg aagcattaga cccagttaac agtatatttt gtgatagtga
     3301 gaaatcattg gctactgcat ctgctatgag tggatgtgtt aactcagaga atactatgcc
     3361 atcatttgaa tcatcaattt gtggttcagt ataacattct agcgttctac gatacattgc
     3421 taatgcataa tgattatcta attcagctat gtaatcgaaa caatttttaa gctcattcat
     3481 atgtgttgta tagctttttt gaattgaatg aaataaaaca taatcaagca tgaaaataag
     3541 tttaactagt ttaattacca tagctccgat atcttcatca ttaacttcag ctaaaacact
     3601 cgttaaatga cgtgctgttc taaattgctt aaaattgact tcaggttgtg gagcatgttt
     3661 aatttttgag atagtatagc cttgttttaa aacattggat gcataaaaga tagattttaa
     3721 gtcatcctca tatgtacgct ttaaagttgc tgataaaatg atattaaata ggcaactcat
     3781 aagaaataat aaaataccaa cttgtgaatt aataaatata actgcaaatg agataactgg
     3841 taaaaacggg catagaaaca atatattttg acgttttacc ggtgtgattt ggtctggaaa
     3901 tgttggataa acagttttac caatcaaagc taaatgatat gttacgtttt ttctaaattc
     3961 agcattatca ttaaataaac taagtaatga tttgttcgta attgaaagca ttccacgtaa
     4021 acaggcatat agcttcattt caccaatagc tgttaaatta taattcatct tatgaaagat
     4081 tgcattcata tttaagtcgg accaagtttt atcatcaact aatgaatgtg attcaaattt
     4141 ttccttgtag agcttatact catagtcgaa atgtgagttc ggtctgataa aagcttctaa
     4201 tggagaaatc gtttgccata atgtttccat agattttaat agacgttgac gactcatgta
     4261 tcgtccaaca attcctatta cagatgtaag tagaataatt gctataacaa atattaaaaa
     4321 tgtttgattt gtgctcataa gtatcccctt atttattttt aataataata aaaatatact
     4381 tcaatattat tattatttca taaagaaaat gtcttaacaa tgaattttga taaaaacaat
     4441 gtcaatagag aacatgcgta cttatcaaca aagagtgaat aacttgtgga taaaaggtgt
     4501 tgataacgtc atttattgtg gatatcaggt gggaaaacag aaaataaatg attgattagt
     4561 gttaatttac ttgtgggtaa ctaggataag tctgtggata gctataatat cagcgttttt
     4621 attgtgaata aaaaaattaa aatttgtaaa aaaataactc taactatagc gattttacag
     4681 ttagagttat gtatattttt atagtttttg ttgtaattct tcccatttag acatagcgtg
     4741 ttctaatttt tgttcgctat cttgtttttg gatagctaat tcgtttgctt tttgtggatt
     4801 gttatacact tctggttggg ttaattgctc gtctatttgt aagatagtag tctctaaagt
     4861 ttctatttca ttttcacact cggatatttg tcgttcaagt ttgcgttgtt cacgtctttg
     4921 ttctttttgg ttatgatacg acgattgttc catagacttt ttatgtgctt gtacgttaac
     4981 tgactgttct tcttggtgcg ctttcaacgc ttccgcttcc tcaacttttt caatgtaata
     5041 ttgataatct cctaaataca tctttccgcc atcaattgtt aaatcaaata ccttgtttgc
     5101 taattggtta ataaagtaac gatcatggga aacaaatagt attgttcctt caaaatgttg
     5161 gagtgcttgc tctaacattt cttttgaatc tatatcgagg tggttggtag gttcatctaa
     5221 aataagtaca ttgtcacgtt gcaacataag tagtgctagt agtaaacgtg ctttttcacc
     5281 accagataaa tcattaatta tctttttaac atcgtcttgt acaaataaga aacgtccaag
     5341 aactgctcga atatcttttt cattcattaa cggatattga tcccacacat aatctaaaat
     5401 cgttttacta gatttaaatt ctgcttgctt ttgatcataa taaccaattt gtaaatttgc
     5461 gccgaaagta atatcgccat taagcgcttt ttgttgatta gcaatagttt taattaaggt
     5521 cgattttcca ataccatttg gcccaatgat tgctatatga tcgcctttag agacctctat
     5581 actcataggt ttggtaattg cagtttgata accgatttct aaatttttta catgcatgac
     5641 gtcattacct gtattccggt caaagccaaa ttgaatattt gcacttttgg catctaacat
     5701 tggtttatca atgcgttcca ttttttctaa aatcttacgt ctactttttg ccattccact
     5761 tgttgaagca cgggtaatat ttttctcaac aaaagtttct aatcgtttta tttctgcttg
     5821 ttgactttca tattcttgca ttcgtttttg ataatataaa tcccgttgct gtataaattc
     5881 ctcgtaatta ccaacatagc gtttgacatc acctaaagcc acatcataaa tttgagtaac
     5941 tattttatct aaaaagtaac gatcatggct gatgatgacg attgcacctt taaaataacg
     6001 tagataatct tcaagccact ttgtcgtttc taaatctaag tggttagtag gttcatctaa
     6061 aagtaataaa tcaggttcgt ttaatagcat ttgagctaaa gataaacgtg ttttttggcc
     6121 accgctaaaa tcattgatag gtttattgaa atcttcttca ctaaaattta acccgtaaag
     6181 tactgtttta attttacttt catattgata tccttctaat tgttcaaatt gattcgataa
     6241 agattcataa cgggacatat gtgttttata tgtatcacta tcgtaatcat ttgcatgttt
     6301 tgataaacaa tctgtttctt ctttgattaa gctttccatt cgtttaatat gttcaaatgg
     6361 ttttgacatt tcttcaaaaa ctgttgcatt agaatttaaa gtcatttgtt gagttaaata
     6421 acctagtttt aagtttttaa tttttgacac atttccagaa tcatagtttt ctacgccagc
     6481 tataattttc atcaatgttg atttaccggc gccatttctt cctacgatac caattcgttc
     6541 accagttttt acttcaaaat caacatcagt aaatatatct tcaccatcga acgattttga
     6601 tatatgattg agttgtaaaa gtatcattcg atcttcacct ttcgtctttc tcaaatttta
     6661 ttaacttatg aaaaaagtcg taattattac attaataacc attttacagt ataaaacgcc
     6721 gtcttgaaac taagatattt atttttaaaa ttcaataatg tataatattt aggtattcat
     6781 tgtaatcaat tatttgtttt ttaataatct aacaattacg ctctaactcg tttgaataca
     6841 tagatataat tcattcaaac acgaaggagg aaatagcgaa tgagtgacca agttaaaatt
     6901 cctcgagcaa ctttaaaacg tttgccgtta tattatagat ttgtcagttc attaaaatct
     6961 aaaggtatag atcgtgtaaa ttcaaaagcg attagcgatg cgttacaaat tgactcggca
     7021 acaattcgtc gtgacttttc atattttggc gaattaggta aaaaagggta cggatataat
     7081 atagatagtt tattggattt ctttaaatct gaactaagcg agagtgacat gatcaaaatc
     7141 gcaattgtcg gagttgggaa cctagggaaa gctttgctca catataactt ttcaatacat
     7201 gacgatatga cgattacaga agcgtttgac gtaaaagaag atgttattgg ccagaaaata
     7261 gggaacgtta ttgttaaaga taacgatgaa ttaataacaa cattgaagaa ggaagaaata
     7321 gatgttgtga ttctaactac accagaaaga gttgcacaga aagttgcaga tgaactcgtc
     7381 caagctggtg tgaaaggtat tttaaacttc actcctggta gaattaatac gccttcagat
     7441 gtgcaagtac atcaaattga cttaggtata gaattacagt cattattatt ctttatgaaa
     7501 aattacagtg aataaatgtg atgttagctt tgaatgatta accttgttat atctttaatt
     7561 aattatagaa cagtctggga ctaagtatgt ctcaggcttt ttctgttatg tttgaaaatg
     7621 aaaggaaaaa ctgttgtgtt gataaatata gtataggtta ataaattaac acgtagagat
     7681 ataatgatga atcgtaatta atcgccttcc ttatatgaat ataatataaa tatcaaaaat
     7741 attgtgtata aggttgaaaa caagaaaatc atcacctata atacatacta aacttatcgg
     7801 aaatgagagg ttaagattat gtggatgatt tttagtggct taattgtcgg gggactctta
     7861 ggttttgtca tgcaaagaac aagattttgt ttaacaggtg gctttcgaga tatgtatgtg
     7921 caaaagaata ataagatgtt ctatgcatta ttaatcgcta ttactattca aagtatagga
     7981 ttattgattt tgacggcaac agatatttta caaattcctg cacatagttt tccaatattg
     8041 ggaacaatta taggttcttt tatttttgga attggaatag tattggctgg aggatgtgca
     8101 acaggtactt ggtatcgcgc tggtgaaggg ctaattggta gttggattgc attagtatta
     8161 tatgctgtta ctgcagcaat cactaaaaca gggattttaa agccagtaat ggataaaatt
     8221 aatcaaccaa cgaatgtaaa tagtgatatg tctcaaacaa ctggcattcc gttttgggga
     8281 ttagtcgtta tattaactat aatcaccatt tttctagttg taagaacact caataataag
     8341 aaagttcgag ttgctgttcc aaaactaaag caacgttata caggtattag atattacctt
     8401 ttcgaaaaac gataccatcc atttattgca gcaattgtaa ttggacttat cgcactctta
     8461 gcttggccaa tgagtgcatc aactggaaga aatgacggtt taggtataac aacgccttca
     8521 gcaaatttag tacacttttt gattacaggt gaaactaaat ttattgattg gggtgtcttt
     8581 ttagttctag gaattttcat tggttcatat attgcagcta gaggatcaag agaatttaaa
     8641 tggcgattgc cagacaagat tacaatacga aacagtgcca ttggtggcat atgtatggga
     8701 tttggtgcgt cagttgctgg tggttgttct atcggtaacg gtttggttga aacggcaacg
     8761 atgacttggc aaggatggat tgcgctagca tcgatgatag ttggtgtatg gacaatgagt
     8821 cattttatct ttgttcgtcc aatgaaaaaa gtacaccaac aatctgcaaa ggttaaacag
     8881 caaacgcaaa tagtatagaa gattattatg caaatgatgt tgatcaaata aaagtgattg
     8941 gaaaaggaga aataattatg atacacgaat taggtacagt aggaatggta tgtccatttc
     9001 cgttaattga agcgcaaaag aaaatggcaa cattgcaatc tggagatgaa ttaaaaattg
     9061 attttgattg cacgcaagcg acggaagcca ttccaaattg ggctgcagaa aatggttatc
     9121 ctgtaacaaa ctatgaacaa attgataatg cttcatggac aattacaatt caaaaagttt
     9181 aacgttatca ttttaacaat aaaatagata ttagattcta tggctacttc cgctaattta
     9241 aaagtgagta agtagtcttt tttgttttag ttcat
//
LOCUS       DALAGF010000106         9009 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.106, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000106 DALAGF010000000
VERSION     DALAGF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.106"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            175..3156
                     /locus_tag="O2692_002278"
     CDS             175..3156
                     /locus_tag="O2692_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCZ4299888.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFVDITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFSKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(3268..4206)
                     /gene="manA"
                     /locus_tag="O2692_002279"
     CDS             complement(3268..4206)
                     /gene="manA"
                     /locus_tag="O2692_002279"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCZ4299889.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(4220..6172)
                     /locus_tag="O2692_002280"
     CDS             complement(4220..6172)
                     /locus_tag="O2692_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HCZ4299890.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTAKHSHLNKPSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMGSTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(6249..8123)
                     /locus_tag="O2692_002281"
     CDS             complement(6249..8123)
                     /locus_tag="O2692_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCZ4299891.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            complement(8363..8821)
                     /locus_tag="O2692_002282"
     CDS             complement(8363..8821)
                     /locus_tag="O2692_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299892.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKSTHK
                     KTETEENDQQDDSADKTDKNQNNGNNVTNDNQPSSPSNVPNNHSTVPSNTQPAQPKDS
                     NSNKNNTHGGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQAQSPKN"
BASE COUNT         2986 a         1459 c         1614 g         2950 t
ORIGIN      
        1 tgtgataata aactatgatt tgttataatt tatcgtatgc tgaaaatagt tgataggtat
       61 caatcgacta aatatcttcc agtaaattga ttatactaat tcacaacgca aaaataaatt
      121 aatttacaaa aaatatataa aaaatatgaa taattcctac ataggagtgt gacaatgaag
      181 aacgcattta aattatttaa aatggatctg aagaaagtag ctaagacgcc agctgtgtgg
      241 attatcttag caggcttagc tattttgcca tcgttctacg cttggtttaa cttatgggca
      301 atgtgggatc catatggcaa cacgggacac atcaaggtcg cagtcgttaa tgaagataaa
      361 ggcgacacaa tcagagggaa aaaagttaat gtcggtaata cgatggttaa tacactcaag
      421 aaaaataaaa gttttgattg gcagtttgta agtagagaga aagctgatca tgagataaaa
      481 atgggtaaat attttgcagg tatttacatc ccatctaagt ttacacatga aattacaggg
      541 acactacgta agcagcctca aaaagcagat gtagaattta aggtgaatca gaagattaac
      601 gctgttgcgt ctaagctaac agatactggt tcgtcagttg tcgttgaaaa agcgaatgaa
      661 caatttaata aaacagtaac tcgagcatta ttagaagaag ctaacaaagc aggtttaact
      721 attgaagaaa atgtgccgac aattaacaag ataaaaaatg cggtatattc agcagataaa
      781 gctttaccta agattaatga ctttgcgaat aaaattgtat atttgaataa ccaccaagcg
      841 gatttagata aatatgccaa tgattttaga aaactaggaa attataaagg tgatatttta
      901 gatgctcaga aaaaattaaa cgaagtcaat ggtgctattc cgcaacttaa tgaaaaggct
      961 aagttgatat tagctttaaa taattatatg ccgaaaattg aaaaagcgtt aaattttgca
     1021 gctgatgacg tgccagcgca gttccctaaa attaatcaag gacttaacat tgcgagtcaa
     1081 ggtattgatc aagctaatgg acagttaaat gatgccaaag gcttcgtcac acaagttaga
     1141 agtagagtcg gtgattatca agatgcaatt cgacgcgcgc aagatttaaa tcgaagaaac
     1201 cagcaacaga ttcctcaaaa tagcgcggcg aacaacgaaa catcaaatag tgcacctgca
     1261 gctggtaatg gtgtagcatc aacgccacca agtgcaccaa gtggcgatac tgcaccaaat
     1321 aataatgtta cgcaaaatac cgcaccaaat agtaataatg cgcctgtatc gactacacca
     1381 caaagtacaa gcgggaaaaa agatggtcaa agttttgtag atataacaac aacacaagtc
     1441 agcacagcta acgagaacac acaaaacatt acagataaag atgttaaatc aatggaagcg
     1501 gcattaacgg gctctttatt atcattatca aataatttag atacccaagc gaaagccgca
     1561 caaaaagata gtcaggcatt acgtaatatt tcgtatggga ttttagcatc ggacaagcct
     1621 tctgatttta gagagtcttt agataatgtt aagtccggtt tagaatacac aacgcaatat
     1681 aatcaacaat ttatcgatac attaaaagag attgagaaga atgaaaatgt tgatttatca
     1741 aaagaaattg ataaggtaaa agcagctaat aatcgaatta atgaatcatt aaggttagtt
     1801 aatcaattaa gcaatgcatt aaagaatggt agttcaggaa ctgctgaagc tactaaatta
     1861 ctagatcaac tttcaaaact agattcatca ttatcatcat ttagagatta tgttaaaaaa
     1921 gatcttaaca gctctttagt atcaatatca caacgtatta tggatgaatt gaacaaaggg
     1981 caaactgcat tatccaatgt tcagtctaaa ttaaatacaa ttgatcaagt cattaacagt
     2041 ggacaagcta ttttaaaaaa tggtaaaaca cgtattgatc gcttacaaac agtattacca
     2101 agtattgaac aacaatacat tagtgctgtt aaaaatgctc aagcaaactt ctcgaaagtg
     2161 aaaagtgatg tagctaaagc tgctaacttt gtgcgcaatg acttaccaca gttagaacag
     2221 cgattaacta atgcgacagc aagtgtgaat aaaaatttac caacgttatt aaatggttat
     2281 gatcaagcgg taggattact aaataaaaat cagccacaag cgaaaaaggc tttatcagat
     2341 ttagctgatt tttctcaaaa taaattgcct gatgttgaaa aagatttgaa aaaagcgaat
     2401 aaaattttca agaaattaga caaagatgat gcagtcgaca aattaatcga cacacttaag
     2461 aatgatttga aaaagcaagc gggtattatt gcaaatccta ttaataagaa gactgttgat
     2521 gttttcccag ttaaggatta tggttcaggt atgacaccat tctatactgc actgtcagta
     2581 tgggtaggtg cactcttgat ggtaagttta ttaacggttg ataataaaca taagagtcta
     2641 gagtcagtct taacgacaag acaagtgttc ttaggtaagg caggattctt tataatgctt
     2701 ggtatgttgc aagcactcat tgtatcggtt ggagatttgt taatcctaaa agcaggagtt
     2761 gagtcacctg tattatttgt acttataacg attttctgtt cgattatttt caactcaatc
     2821 gtatatacgt gcgtatcatt acttggtaac ccaggtaaag ccattgcaat cgtattgctt
     2881 gtattacaaa ttgcaggtgg tgggggaaca ttcccaattc aaactacgcc acaatttttc
     2941 caaaacattt cgccatactt accatttacg tatgcaattg attcattacg tgaaacagta
     3001 ggcggtattg ttccggaaat cctaattaca aaattaatta tattaacgtt atttggtata
     3061 ggattcttcg ttgtaggttt aattttaaaa cctgtaacag atccattgat gaagcgcgta
     3121 tctgaaaaag ttgaccaaag taacgttaca gaataaaaat taaatccaca cattagggtt
     3181 atagctcctt aatgtgtgga tttttatgtt tttagacaga agagatagta atttctgtct
     3241 tttatgggac ggttgttatc attgctatta tccaggatga cttactatag cactaatatt
     3301 accgacaaag tgaatatcct cgtcttccgt agttaaaata aagctagaac ctttttggat
     3361 gtcatagtgc ttatcgttta ctgttaaagt accagtacca tcgataattg taactaagca
     3421 ataagcatgt ggtttattga attttaaatc tccatgaata tcccatttat atactgcaaa
     3481 atattgatta tctacaaatt gagttacagt gtgtgtgtcg atgtgagttg ttataggagt
     3541 agtatttggt tcatgattgc ctaattcaat cacatcttta ctttgctcta agtgcaaatc
     3601 acgcaattga ccattttgat ctcgtctatc atagtcataa atacggtatg tcgtatcgga
     3661 ggattgttgt gtctctaaaa ttaaaatacc cgaaccaatg gcatggacag tgccagcagg
     3721 aacataataa aagtcaccgg gcttaacagg tatacgtttg aaaagactgt caaattcatg
     3781 attatcaatc atgtctatta acgtctgttt attatgtgca tgtacgccat atataatttc
     3841 agcacctggg gctgcatcta aaatatacca acattctgtt ttacctagtt cgccttcgtg
     3901 ttttaaagcg tagtcatcat ctgggtgaac ttgaacagat aatttatcat tggcatctaa
     3961 tactttagtt agcagaggga aactatctcg tgaatcatta tcgaataatt cacgatgttg
     4021 tgaccaaagt tgatctaggg tcatatcctt gtatggacca ttgataattg tattaggacc
     4081 atttggatgt gcagaaattg cccagcattc accagttgtt tcattaggga tatcatagtt
     4141 aaatgctttt aatgcatgac cgccccaaat tctgtcttta aaaacgggtt gtaaaaataa
     4201 tgccatagtt aaaactcctc tatattttca ttaataagtt ataaatttct gtagtactgt
     4261 ttgcattaat tagtgattgg cgtgtctcat cattcattaa cgctttagat aagcgctgaa
     4321 gtatttttaa atgtgtatcc tgactgttgt ttggtacggc aattaagaat atcaattgag
     4381 gtagactacc atctagactg tcccatttaa caccatgatt atttttcata acagctacaa
     4441 tcggttgttt tacaacatca gactttgcat gtggaatggc cacgttcatg ccaatagctg
     4501 tcgtagaccc catttcacgt tctagtattg cattttttaa atgcgatgtg tgctctacat
     4561 aacggcaaat tttaagttta tgaatcaaca tatcaattgc ttcgtttcga gacatgtcgt
     4621 gatcagtaat tatcatagtt tgttgatcaa aaacatgaga aggtttattg agatgtgaat
     4681 gtttcgcggt gttatctaca ttgtcaacct ctgtatcatg ttgtgtaata tctgtatcat
     4741 gaagttgcgt gtgttgcgct ggtgcatcta ctgctataac tggtgtattg cgttttaata
     4801 atagtacagt agtcattgtg acaagactac ctactatcac tgcaaagata aaccataata
     4861 catgatcaat accacctaat acagccacga ttggacctcc atgtgcgact ctatcgccga
     4921 caccaccaat ggctgcaatg actgatgcaa tcattgcacc aatgatgttt gcaggtataa
     4981 tgcgcaatgg atcttgggct gcgaaaggaa tagcaccttc agtaatacca aatagtccca
     5041 tagtgaagga agccttaccc atttctcttt cggaatgatt gaatttatac ttttgaacaa
     5101 acgttgctaa acctaaaccg attggtggtg tacatacagc aactgcgacc atacccataa
     5161 cggcgtaatt accttcagca ataagtgctg agccaaataa aaatgctacc ttgtttactg
     5221 gaccgcccat atcgaaggca atcatcgcac ctataatcat cgcaagtata ataatattag
     5281 caccttgcat actttttaac caggttgtta atgcctcaaa aatattagaa attggtgcac
     5341 cgattaaaaa tataaatatc aatcctacaa cgaccgatga aataatggga ataataatga
     5401 taggcataat tggtgccatt gcttttggaa ctttaatatc tttaatccac tttgcgatat
     5461 aacctgctaa gaaaccagca acaataccac ctaaaaatcc tgcgcctgca tcactgccat
     5521 aaaaactacc gtcagcagcg atagcgccgc caatcatacc aggaacaaga ccgggcttgt
     5581 cagcgatact aacagcgata tatccagcta gtattggaac cataaattta aaggctaaac
     5641 taccaatgtt ttcaatggat ttccaaaatg aatcatctgg gatgactaat ccttttgatg
     5701 tcgtttcacc gcctagagtc agcgcgatgg cgataaggag tccaccaact acgataaaag
     5761 gaaccataaa cgatacaccg ttcattaaat gttgatacac catttgaata ccacttttag
     5821 acttaccgcg atctttcgaa tgataatttg tttcagattg ataaataggc gcatcttgat
     5881 taatgatacg ttgaattaga cctctcggat tatgaatccc ttcgcgaaca ttttcattaa
     5941 tcaaccgttt accaacaaat cgggacagat caacttgttt atcagctgca attatgacac
     6001 cgtcagcttc ttcgatgtct tgcgtagtta aaacattttc agcaccaaca ccgccctgtg
     6061 tctctacttt aatatccaca cccatttctt ttgctacctg ctcaagcttt tcttgagcca
     6121 tatatgtatg tgcaatgcca tttgggcatg aggtaatagc tacaattttc ataaaatcat
     6181 ctccttttct atattgtaag cgtattctcg atactaaaaa aaagaataat taccgttact
     6241 agtggcaatt attcttgtaa gtattcaaat aactgttgct ttaaactatg atcatctaaa
     6301 ctacataaat ggttcactga atcatcatcc aagttagcaa ttaattgcat catttgtttt
     6361 gtaaaagctt tgtctttatg cgaaatcgct aagaaaaaga caagtttgac atcgtgttgt
     6421 cgccaaggaa aaacatcttt tgtgcgaaaa ataagcacat gtgattgtaa aactttttca
     6481 ggatctccat gaggaatcgc cataaaatta cctatgtatg tagaagatga tttctcacgc
     6541 tctaaagctg attcgatata tccttctaca atcgcatgat gtgcttgtaa tattttttga
     6601 gcttcttcaa aaatttgcac agtatgccgt gatttttgtt cagtatttac gacaaggaaa
     6661 ttgacagtgt ccatatgatg atgtgcttga accggatttt gcttttgctt cacaacgtgt
     6721 ctgattttgt gacgatcatc ttcagaaaat aatggtgcaa ccttgatagt cgtcaggtgc
     6781 ttaggaagta tgtttagcgt ttgtttagga atatcatggg tcgttattaa taaatctaca
     6841 ttgtcaaagt gatagtgtgt tatattttct agtttaatcg tatttatcac tgacaactct
     6901 tcggataagt tatttatttt agtttctaaa aaattcgaca cacctagacc ataataacaa
     6961 gcaatgacta catttaattg tgttttggta cgacgctcga tggcagcttg aaaatgaatt
     7021 gttaaaaatg caatttcatc ttcgctcatc tctatatcag tatcaattgc taatttatca
     7081 atcgcttcaa aaagtgtgtt aaacacaaag ggatagagtt ttttaatctc tataactaaa
     7141 ggattgttta aataaatgtt ttgagtgata cgtaaatatg ctttactaaa atgattatat
     7201 aaattttgtt gtaaaatcga atcttcattg aaaggtacat gaatacgttg ctgcatcaat
     7261 tcgattaagc gatcaatata actttgtata aatatacgtt ctatgccaat atcgagttta
     7321 ttaaaatgat aagcaataaa gaatgaaaac atattgatta ctttttcgtt caagtcataa
     7381 cctaatcttt cgttgatttg cttaatgcaa gattgagata tcaattttga atcgtggttt
     7441 aacgtgtcta gctcacttaa agattgctta cgtttaatga ttaaaatgag atgaattaaa
     7501 agctgttgta tttgaatatc agttgtttca atactatgtt gttgaagtgt ctcttgtata
     7561 atatgcgaaa tcatcctttg gtgtgaatca ggtaattcat ttaaaattag gtcttcaaca
     7621 tgtacatgcc ctgatgataa ttgatttaaa tggatgatgg cattagtgat atcattatct
     7681 gttccatcga ccacaatacc ttgttttttc ttgattgtta aagcgatatt aaatttttga
     7741 caccattgtt gaattcgaac gaaatagtcg ttgagcacag ttttagataa tagataatct
     7801 tgctctagtt gttgcaatgt ctgcgattta ttgtgcatta gcagttgata cgccatagat
     7861 aataatagtt tttctttata tgattgaata tgcgtgtaac gttcagttat cgtttctaag
     7921 gtatactgag atgtatttaa ttgatatcct ttagatttaa tacttacgat gatatcatcc
     7981 ataaaattgc tattgataac atggatatca ttgcgaactg tgcggtttga aacattgaca
     8041 tgtttagcaa tttcattaga actaatgtgc tttgatggat ttttaataaa aaactggagt
     8101 agttttaagt gtctatcaag catttaacat gtacctcctt tctaaatttt tcgtgtaagc
     8161 gtttttaagg gtgtattaat attattaaac atgagagctt atacatacgt caatgacatt
     8221 aaagcgaact tttatatgat tttaacagag tgcgaattat gcaaataaag aacagcagta
     8281 agatatttca aatagaaaaa tatctcactg ctgtttttct gaatttatgc atctggtact
     8341 tgtggacgta tcaggcaaat gattaatttt taggtgattg tgcttgaggt gtttgtttag
     8401 aaggcgtatt ggtattattt gatttattag caggtggtgt gttctgttgt tgattatttt
     8461 ggttgtttgt tgacgattga ctgccaccac cgtgagtatt atttttattt gaatttgagt
     8521 cttttggctg agctggttgc gtattcgatg gtactgttga atggttgtta ggaacatttg
     8581 acggtgaaga tggttgattg tcattagtaa cgttattgcc attattttga tttttatctg
     8641 ttttgtctgc actatcatct tgttgatcat tttcttcggt ttctgtcttt ttatgcgtag
     8701 atttattttc ttgttcttgg ttattacttg atgatgtaga atgatttatt gcagcataaa
     8761 ttgcgaaccc aagtgctccg attaatacga taattacgat ggtaataaga agttttttca
     8821 aatataacgc ctccatgtta atgaaaagta gttgatacgt gaagctaaat aatttatttc
     8881 aatattgcca taatcatttt aaaaaataaa tattagaaaa gtaggcgata ttaatatttt
     8941 cattgttttt tgtgtacttc aattagtata acaaaactgt tatgtattta taaatataat
     9001 cattagtat
//
LOCUS       DALAGF010000107         8879 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.107, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000107 DALAGF010000000
VERSION     DALAGF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.107"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(555..2042)
                     /gene="lysS"
                     /locus_tag="O2692_002283"
     CDS             complement(555..2042)
                     /gene="lysS"
                     /locus_tag="O2692_002283"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCZ4299893.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            complement(2095..2187)
                     /locus_tag="O2692_002284"
     CDS             complement(2095..2187)
                     /locus_tag="O2692_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299894.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            complement(2581..3057)
                     /gene="folK"
                     /locus_tag="O2692_002285"
     CDS             complement(2581..3057)
                     /gene="folK"
                     /locus_tag="O2692_002285"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCZ4299895.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK"
     gene            complement(3054..3419)
                     /gene="folB"
                     /locus_tag="O2692_002286"
     CDS             complement(3054..3419)
                     /gene="folB"
                     /locus_tag="O2692_002286"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HCZ4299896.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            complement(3397..4200)
                     /gene="folP"
                     /locus_tag="O2692_002287"
     CDS             complement(3397..4200)
                     /gene="folP"
                     /locus_tag="O2692_002287"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCZ4299897.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAINRVKAMIDEGADII
                     DVGGVSTRPGHEMVSLEEEMNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            complement(4416..5348)
                     /gene="cysK"
                     /locus_tag="O2692_002288"
     CDS             complement(4416..5348)
                     /gene="cysK"
                     /locus_tag="O2692_002288"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCZ4299898.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            complement(5527..6408)
                     /gene="hslO"
                     /locus_tag="O2692_002289"
     CDS             complement(5527..6408)
                     /gene="hslO"
                     /locus_tag="O2692_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCZ4299899.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            complement(6637..8730)
                     /gene="ftsH"
                     /locus_tag="O2692_002290"
     CDS             complement(6637..8730)
                     /gene="ftsH"
                     /locus_tag="O2692_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCZ4299900.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHQQA
                     PNIEKPYDPNHPDNK"
BASE COUNT         2694 a         1848 c         1158 g         3179 t
ORIGIN      
        1 aataacttac ttattcatga tacacgctta attaataagg tgcaatagtt aattttacac
       61 tttgatgtaa actttgtcac atcagctttt agtgtgtttc gtctgttttt ggcgactttt
      121 atatattact tcattcaatt gataaagtca acacttttaa tcaacttttt tattcttcaa
      181 aactgacatt tctcgtgccc tctaattcat aacactttta gtattctatc atacacatat
      241 gtacaaaacc atacacattc tatacaattt taaagtactt ttatagctga ttaagataaa
      301 ttatactaat atagcaattt ttgttataag atatctcaat gtctatcgtt aacatttctc
      361 atccgaacta ctctatatac gtatcgtcat tttcatctat cagccaacta ccttgtttaa
      421 cagtcaaaaa tagcaccact atcgttaact attactatca aaaacattca aacacgaact
      481 aattaccata attaagaaag ccctcgcata cacatgttgt atacacgaga gctcttacta
      541 acaatcaacg tcatttattt ttgtctcata taagggaata ataatacgtc tctgattgat
      601 ggagagtcag ttaataacat aaccaatctg tcaataccga taccaagacc acctgtcgga
      661 ggcataccat attctaacgc ttcaatgtaa tcttcatcca tttcatgcgc ttcatcatta
      721 ccttgcgctt tttcaacaag ttgcgcttca aaacgacctt tttgatcaat aggatcattt
      781 aattcagtaa atgcatttgc atgctctcta cctacaatga acaattcgaa acgatcagta
      841 aatctaggat cttcaggatt tttcttcgct aaaggtgaaa tttcagtcgg atgaccatag
      901 ataaacgttg gctgaataag tgtttcttca actttttgct caaagaattc atttaaaata
      961 tgaccatatt tcattgtatc tttaatttca ataccatgtt ctttagctaa agctttagct
     1021 tcttcatcac ttttaacttc ataaaaatct acaccagtag cttcttttac agcatcaaca
     1081 atatgcaaac gagtccaagc agattctaaa tcgatcgttt ccccattgta ttgtactttt
     1141 gcagaaccta acacttcatt ggcaatatgt ctcaccatag attctgttaa atccataatg
     1201 tcatgataat ctgcatatgc ttcatataat tcaatcattg tgaattcagg gttatgtcta
     1261 gttgatacac cttcattacg gaatactcta ccaatttcat atactttttc aagtccaccg
     1321 acaattaaac gttttaaatg caactcaata gcaatacgca tgtataacgt tgcatctaat
     1381 gcattatgat gtgttacaaa tggtctagca gctgctccac cagcaatttg gtgcatcata
     1441 ggtgtttcta cttccaagaa acctttatta tttaaataat tacgcatttc ttgaatgatt
     1501 ttactacgat taataaatgt acgagtgcta tcttcgttcg taattaaatc taaatatctt
     1561 tgacgatatc tctgttcaat atcctgtaaa ccgtggaatt tatccggtaa tggtcgcaat
     1621 gatttagtta gtagcgtgaa tttcttcgct ttaaccgata attcgccagt atttgttttg
     1681 aacattacac cttcaacacc aacgatatcg cctaaatcag catttttcca taaatcaaat
     1741 tcgtcatcgc caacttgatc tttacgaacg taaatttgaa tttgtccagc taagtcctga
     1801 acgtgtgcaa atcctgcttt acctttacca cgcttagtca ttaatcgtcc agctatagcg
     1861 acatgactat ccgcttcttt ttctaccaat tcttctttag aatactggtc ccactcttct
     1921 ttcaaatcac tagataaacc tgaacggtca aatttagaac caaacgggtc tataccaaga
     1981 tcatataatt cttgtaattt ttgacgtcga accaacattt ggtcattcat ttcttctgac
     2041 ataactttct ctcctttaac ttgttgtaat cctttagtat tccaactggt acactcaaac
     2101 attttccttt aaagttttat aaaatatcag acatttttgc tcaacataca tctctttcga
     2161 tgtttctaat actttatcat taaccattac accttaaagt tatgatgtgg catgttttct
     2221 tatattcata acatcaattt tatcactttt ttaattttac aagagcttgt aaatgtaata
     2281 aagcaatttc tgttttcgtt tatgaattac ttattcattt aaaatacgat tatcattaat
     2341 acaaccctaa gtaaatgtat aaaaattatc ttccacaaac ttcaacaaaa gcctaaataa
     2401 attacagcaa tttatcaaat attgcttact ttgattttat gaaataactt aattctaaca
     2461 catactaaat catcatatac taattcgaaa tcaaatgcat ttagagataa tcaaaatgcg
     2521 gaaacatctc caataatcaa taatctattc ccaataaata tgaatgttct caacaataca
     2581 ttatttatat ctctttacac tgtcatcgac aaaaactaaa tctttcactt tcaatttcga
     2641 acgtggttct acgacatttg ctgctatatc atttaatggg attaaaacaa atgcacgttc
     2701 attcattctc ggatgtggca ccgacagttt tggtaaatct atcatttctt ctccatacaa
     2761 caaaatatcc acatctaaag ttctaggacc ccatcgttcc tttctaatac ggtgtaaaca
     2821 ttcttctgtc ttcaaacaac attccaacag ttgtaatact gtgagtgttg tttgaatttc
     2881 aacacacaaa tttaaaaagt taggttgctc agtataccca actggtgctg tttcataaat
     2941 cggagaaata ttagatacgt taataccatc atattcattc aaaatcttta tagcatcgtt
     3001 taactggctt tctctatcac caatattact acctaatcct aagtatgctt gaatcattta
     3061 ttctccctca ctatttcgat acctactcca tcataatgac ccggaatcgg tgggttttct
     3121 ttagtgattc tcactttcgt ttccattaca cgattatatt gtgaatttat acgatttgca
     3181 atacgttcag ctagatgctc aagtaaatta acggccttac cttccataat tgatttaacc
     3241 tcttcgaaca cttcaccata atgaactgta tcaataacat tatcagtacg cccagcttca
     3301 gctaagtcta ctttcaaagt tacatccact ttgaaaattt gccctatttc attttcagct
     3361 gataaagcac catgatatcc ataaaagcgc atacctttaa gaaagattgt gtcttgcatt
     3421 ttcattctcc tttaaaaaat ctataccttt agctaattta gcattcaact cgacattatg
     3481 aacgcgtact gctctaacgc ctttcataat accatatgca gtcgtagctg cagttacttc
     3541 atctctttca accggtgttg tatcataacc catcatctct ttagtgaaac gtttccggct
     3601 tgtcgctaat aaaactggat attctgttgc aacaagttca tccagtcttg ccataacttc
     3661 ggcttcttca tttctagttt tagcgaaacc tatacctgga tctagccaaa ttttatttga
     3721 aggtatacca gctattttag cttgatgtgc ttgtgctaac aaagatgtta acatttcttc
     3781 gacaaccggt tcatcacgat taccatttcc attatgcatt aaaataattt ccgcgtcata
     3841 tttagctaca atttggaaca tacgatgatc atacagaccc gcccattgat cattaatcat
     3901 atcaacgcct aattttaaac atgcttcagc aacctcactt cgaaatgtat cgactgaaat
     3961 ttttacatca aaaccgacaa tagcttcaac aacaggtaat actctgttca tctcttcttc
     4021 taatgaaacc atttcatgac cgggtctcgt tgaaacacct ccaacatcta taatgtcagc
     4081 accttcatct atcatggctt tcactctatt tatagctgat tcaacattat taaattttcc
     4141 accatctgag aatgaatcag gtgtgacgtt taatatgccc ataatttttg ttttagtcat
     4201 gttaaccacc ctttaattat gtattgttta actctaataa aatttactcc tgctatttac
     4261 tagttattag tatgtacgtt tattatctta tcattcactg gatattttta tcagaaaaat
     4321 gtttaattta acgctcatat ttttaaaaat atacatggta gaaaaaaaga gcaagttatc
     4381 tcaaaaagat aactcactct tttaaatgac attaattagt catcgaatga atataaaggt
     4441 gttgataagt agcgttcacc attactcggc aatactgtta ctactgtttt accttttcct
     4501 aattcttttg ctttttgaat ggcagcataa atcgcagcac ctgatgaaat acctgctaaa
     4561 ataccttcct ctttagcaac tcgacgagac atttccatcg ctgtatcatt tcctacttta
     4621 ataatactgt catagatttc tgtattcaaa gtgcctggaa taaatccagc acctaaacct
     4681 tgtaatttat gtggacctgg ctcaccaccg ctcaatactg gagaagcctc aggctctata
     4741 gcaacaattt cgatgttagg atattctttt ttcagaactt tacctacacc agataacgta
     4801 ccaccagtac caacaccagc taggaacgca tcgatagttt tcccttcaaa ttgttgtaat
     4861 aactcaggac ctgtagttaa ctcatgaact tcagggttcg cagggttttc aaattgttgt
     4921 ggctcgaagt aaccatgttc ttctttcaat tctttagctt ttttaattgc acctttcatc
     4981 gcttctgatc caggcgttaa aactaattcc gcaccgtatg cttttaataa attacgacgc
     5041 tcttggctca ttgtttcggg catagtaaat actgctttat atcctttagc agcacataca
     5101 aatgctaaac cgatacctgt attaccactt gttggttcta caattgtatc gccaggttta
     5161 attttgcctt ctcgctctgc tttttcaatc attgctaaag caattctatc ctttacagaa
     5221 ccacctggat tttgatattc caattttaca taaacatctg ctgcattgtc atctactaca
     5281 tttctcaatt tgactaccgg tgtaccgcca ataatttgag taatattatc tactggtttt
     5341 tgtgccatta agaacactcc tttttagtta gttgttgaaa tcttagttta tttatcggat
     5401 atagaattat tttatcaaat aaattctaga aagcaaacaa aatcactaag gattatacaa
     5461 ctatttagtt ctacatttca ctaaaccgct aaaaataaac attttagcgt attgatttaa
     5521 attaaattac gctaaacttt ctagcaacac gtttaattct tcttcagtat atttatattt
     5581 atttccacag aaatgacata ctgcttcagc accatgatct tctttaatca tattttgaat
     5641 ctcagcctcg cccaatcctt taatagcatt taaaaatttc tcatgactac aattacattc
     5701 aaattgaaca ggcattttct ctaaaatttg cacatggtct tcacctaaga tttcgtttag
     5761 taatccttct ggcgttaatc cttgttcaat taatttagaa actggtgtca tttcactaat
     5821 tgctttttct aattttgaaa ttgtttcatc tttggcacct ggcataactt gaatgataaa
     5881 tcctcctgct gctttaatcg tattatcagg atttaccaat acaccaagac ctaccgatga
     5941 aggtgtttgt tcacttgtag cataataata agtaaaatct tcaccaagtt ctcctgaaac
     6001 aattggactt gctccagaga aatagtcttt cattccaacg tctttaacaa ccataataga
     6061 tccatttgtc cctaccgctc gtcttacatc aagtttacct tgctcattta atggaaaatg
     6121 agtttgtgga tggtctacat aagcacgcac ctcgccttta gcatttgcat cggcaataat
     6181 tcgtccaata ggtccttggc catctacagt gacagttaat ttttgatcac ctttcaacat
     6241 tgcgcccatc atagctgttg ctgtcattgt tcttcccatt gcagcagatg ctgtcggcca
     6301 tgtataatgt ctcgtttgtg cttcttgaac agtttcagtt gtcaaagcag cataagccct
     6361 aatctctcca tcaaatgcta atgctttaac aatataatcg tgtgtcatta tttcaatctc
     6421 ctctattact ctatatttaa aaaattactt tacttcataa aatgcaacaa ttgtacttat
     6481 tctacaccca tcattctaaa taatgaagta acttgtttta caattatttt ctgctataac
     6541 aattcaacga cttaaaatct aatacgtatt ttcaaaaacg ataaaagtac ctctttctat
     6601 aactttatca tagaaagagg tactgaatat aatcgattat ttattgtctg ggtgatttgg
     6661 atcgtaaggt ttttcgatat ttggggcttg ttgatgtgct ggttcatctt tttcatcaga
     6721 tttatcagct tcttttttat cctcagcaat atctttttct tctttacgat cttcactttc
     6781 gtcacgttgt ccatcttcta attgctcttt acgaatctct tcataagatt taccgaattt
     6841 accatcatta aattcagaat cttcatcttt aacaacttta gctgcatcat aatcaatttc
     6901 aggtaattta ccttcgtaga ataatgattg aatttgttca gcaactaatg tttcttctgt
     6961 taataatgtt tcagcaatta aaattaattg ttctttgtgc tctaataaaa tttgtttaca
     7021 acgttcgtat tgttctttaa cgattcgttg aacttcttta tcaatttcat atgcgatttg
     7081 gcttgaataa ttaggctcac cttgcatatc tttacctaag aatacttgac cattgctatg
     7141 accgaactgt aatggtccta attttttact cataccatat tgcgtaacca ttgagcgtgc
     7201 gatttgtgtt gcacgttcga agtcatttga agcacctgtt gatacttcgt taaagttaat
     7261 atcttctgat acacgtccac caagtaaacc acagatttta tctaataact cttgttcagt
     7321 cattaagaaa cgatcttgtt taggtagcat cattgcataa ccacctgctt gtccacgtgg
     7381 aacaatagta actttatgca ctacttctgc ctcatcaagt accataccga taattgtatg
     7441 accagcttcg tgatgagcaa caatattacg ttctttctta gaaataactc gagatttctt
     7501 agcaggtccg gctataactc tatccgttgc ctcttcgata tctctcatgt caattttctt
     7561 tttaccttca cgtacagcaa ttaaagatgc ttcatttaat aagttctcta aatcagcacc
     7621 tgagaaacca ggtgtacgtt gtgaaattgc ttttaaatca accgtttcat caagtggttt
     7681 gtttttagca tgaacatgaa gaattgcttc acggcctttc acatctggac gaccaacttg
     7741 aatttgtcta tcaaaacgac ctggacgtaa taaggctggg tcaaggatat caggacggtt
     7801 tgtagcagct atcataatga taccttcatt ttcaccgaaa ccatccattt caactaataa
     7861 ttggtttagg gtttgttcac gttcatcatg accgccacca acacctgcac cacgttgacg
     7921 accaacagca tcaatctcat cgataaagat gatacaaggc gcgtttttct tagcattatc
     7981 gaataagtca cgaacacggc tcgcaccaac accaacaaac atctctacaa agtctgaacc
     8041 actaatagag aagaatggtg cgccagcttc acctgcaacc gctctagcaa gtaatgtttt
     8101 accagtacct ggaggtccaa caagtaagac acctttagga atcctagatc ccatttcttt
     8161 gaatttttta ttatctttca agaaatcaac aatttcaatt aattcttgtt tttcttcatc
     8221 tgcccctgct acatcagaga aacgaacacg acgtttatta ttatcgtaca tttttgcttt
     8281 agatttacca aagttcatca tacgaccgcc actaccgcca ccttgtgctt ggcttaggaa
     8341 gaaaataaat aataacgcta tgactacaac tggaattaat gttgaaagta tactcacaaa
     8401 gacactttgt ttttcttctt ctttaatcgt taattttaca ccgttttgct ttttagcagc
     8461 atcagtaatt ttttgtaatt ctttttcgtt gttatataaa atagttgatg aatagtcttc
     8521 atcatttttc gttttaccac ttaccatata gacattttgt tgtggttgga tttctaaagt
     8581 tttaaggtca cctttttcca acttctcagt aaattgatta tatgtaagct gtttcggcat
     8641 atttccatta ccgtttaaat atgaaaatag accaaaaata ataacgccta ttattacgat
     8701 aactagcaca ttgcgaaaag ctttctgcat gcgtcatttc ctcctacttc ccataataaa
     8761 actaacaaaa attgtaacat atgacatgtt ttataagcta gtaattgagc ttaacatcaa
     8821 tgtcattcac acccgtatta gttttatcaa tctctatatt tttcaacttt tcaagacat
//
LOCUS       DALAGF010000108         8844 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.108, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000108 DALAGF010000000
VERSION     DALAGF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8844
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.108"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            268..1224
                     /locus_tag="O2692_002291"
     CDS             268..1224
                     /locus_tag="O2692_002291"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCZ4299901.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1424..1903
                     /locus_tag="O2692_002292"
     CDS             1424..1903
                     /locus_tag="O2692_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCZ4299902.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1918..2757
                     /gene="fakB2"
                     /locus_tag="O2692_002293"
     CDS             1918..2757
                     /gene="fakB2"
                     /locus_tag="O2692_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCZ4299903.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2843..3376
                     /gene="msrA"
                     /locus_tag="O2692_002294"
     CDS             2843..3376
                     /gene="msrA"
                     /locus_tag="O2692_002294"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCZ4299904.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            3369..3797
                     /gene="msrB"
                     /locus_tag="O2692_002295"
     CDS             3369..3797
                     /gene="msrB"
                     /locus_tag="O2692_002295"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCZ4299905.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3809..4309
                     /locus_tag="O2692_002296"
     CDS             3809..4309
                     /locus_tag="O2692_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCZ4299906.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            4309..4530
                     /locus_tag="O2692_002297"
     CDS             4309..4530
                     /locus_tag="O2692_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCZ4299907.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            4722..6212
                     /locus_tag="O2692_002298"
     CDS             4722..6212
                     /locus_tag="O2692_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HCZ4299908.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDKALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            6612..7121
                     /locus_tag="O2692_002299"
     CDS             6612..7121
                     /locus_tag="O2692_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4299909.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            7133..8203
                     /locus_tag="O2692_002300"
     CDS             7133..8203
                     /locus_tag="O2692_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCZ4299910.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTLIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            8220..8834
                     /locus_tag="O2692_002301"
     CDS             8220..8834
                     /locus_tag="O2692_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCZ4299911.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFVYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGLFCIILSTLLLRNKLIN"
BASE COUNT         3307 a         1208 c         1578 g         2751 t
ORIGIN      
        1 aacataatat tcaaatcatg tgttaacctc ttttttaaaa ttttttagca ttaaagttat
       61 agatttgggt aaacaattac caattggaaa catatatcac gttacgatgg ggtaggtact
      121 taatcagcat tttataaata aagtaacgga attcatgata ttaatatcat attcctaaaa
      181 tgagtgataa caaaatgcta cataaagtta agttatatca aactaaatat acatactata
      241 aataatgaaa atgaggtgtt atcgcatatg ttgaattcat ttgatgcagc atatcacagt
      301 ctttgtgaag aagttttaga aataggaaat acacgaaatg atcgcacaaa tacaggtacg
      361 atttcgaaat ttggtcatca acttcgcttt gacttatcta aaggatttcc actattaacg
      421 acaaagaaag tttcttttaa attagtagca accgaattat tatggttcat taaaggagat
      481 acaaacatcc aatacttatt aaaatataat aataatatat ggaacgaatg ggcttttgaa
      541 aattatatca aatcagacga gtataaaggt ccagatatga cagatttcgg gcatcgtgca
      601 ttgagtgatc ctgaatttaa cgaacaatat aaagaacaaa tgaaacaatt taagcaacgt
      661 attcttgaag atgatacatt tgcgaagcaa ttcggggatt taggaaatgt ttatggtaaa
      721 caatggcgag attgggttga taaagatggt aatcattttg atcaacttaa aacagtaatt
      781 gaacaaatta agcataatcc agattcaagg cgacacatcg tatctgcatg gaatccaaca
      841 gaaattgata caatggcact tccgccttgt cataccatgt tccagtttta tgtccaagat
      901 ggtaagttaa gttgccagtt ataccaacgt agcgcagata tctttttagg tgtgccattt
      961 aatatcgcaa gctacgcttt attgacacac cttattgcca aagaatgtgg acttgaagtg
     1021 ggtgaatttg tgcatacatt tggagatgca catatttatt caaatcatat tgatgcgatt
     1081 caaacacaat tagcacgtga aagcttcaat cctccaacat taaaaattaa cagtgacaag
     1141 tctattttcg acataaatta tgaagatttg gaaattgttg actatgaatc acatccagca
     1201 ataaaagctc caatagcagt gtagtcattg catagttagc taaccatata gacatcaaaa
     1261 tgacatcata gtattttcaa gtgcaaaaaa gtactttttt gtgttaaacg ttttcataaa
     1321 ttatgcaaaa tcattatttc tatcacactt tatgataaaa attgtgttaa attaaagata
     1381 acttagtaat aaaaaatgaa atgatagaag aaggaggata attatgactt tatccattct
     1441 agttgcacat gacttgcaac gagtaattgg ttttgaaaat caattacctt ggcacctacc
     1501 aaatgatttg aagcatgtta aaaaattatc aacaggtcat actttagtaa tgggtcgtaa
     1561 gacatttgaa tcgattggta aaccactacc gaatcgtcga aatgttgtac ttacttcaga
     1621 tacaagtttc aacgtagagg gcgttgatgt aattcactct attgaagata tttaccaact
     1681 accgggccat gttttcatat ttggagggca aacattattt gaagaaatga ttgataaagt
     1741 ggacgacatg tatattactg ttattgaagg taaattccgt ggtgatacgt tctttccacc
     1801 ttatacattt gaagactggg aagttgcctc ttcagttgaa ggtaaactag atgagaaaaa
     1861 tacaattcca catacctttc tacatttaat tcgtaaaaaa taagggggaa aacgaccatg
     1921 acaaaacaga ttatagtaac agactcaaca tccgatttat ctaaagaata cttagaagca
     1981 aacaacattc atgtaattcc tttaagttta actattgaag gagcttcata cgttgaccaa
     2041 gtagatatta catcagaaga atttattaat catattgaaa atgatgaaga tgtaaagaca
     2101 agtcagccag ccataggtga atttatatct gcttatgaag aactaggaaa agatggctct
     2161 gaaatcataa gtattcatct ttcttcagga ttaagtggta catataacac tgcttaccaa
     2221 gcaagtcaaa tggtagatgc taatgtaact gttattgatt caaaatctat ttcttttggt
     2281 ttagggtatc aaatacaaca cctagtagag cttgtaaaag aaggtgtctc aacttctgaa
     2341 atagttaaaa agttaaatca tttaagagaa aacattaaat tatttgtagt tatagggcaa
     2401 ttgaatcaat taattaaagg tggcagaatt agtaaaacaa aaggtttgat tggtaatctt
     2461 atgaaaatta aaccaattgg tacactagat gatggtcgct tagagcttgt gcacaatgcg
     2521 agaactcaaa attctagtat ccaatacttg aaaaaggaaa ttgctgaatt tataggagat
     2581 catgaaatca aatccattgg tgtcgcacat gctaacgtca ttgaatatgt tgataaattg
     2641 aagaaagttt ttaatgaagc ttttcatgtg aataattacg atataaatgt aactacacca
     2701 gttatttctg cacatactgg tcaaggtgcg attggcctcg tagtccttaa gaagtaaatt
     2761 taatcttttc agtgttaatt acttccattt caatccttta tagactaaat ttataattag
     2821 atagatagag gaggtaattc atatgacaaa agaatatgca acattagcag gaggatgttt
     2881 ctggtgcatg gttaaaccat ttacatcata tccaggcatc aagtcagtcg tatctggtta
     2941 tagtggcggt catgttgaca acccaactta tgaacaggta tgtacgaatc aaaccggcca
     3001 tgtcgaagca gtacaaatta cgtttgatcc agaggttact tcctttgaaa atatattaga
     3061 catatatttc aaaacatttg acccaactga tgatcaaggg caatttttcg atagaggcga
     3121 aagctatcaa ccagtcattt tctatcatga tgaacatcag aaaaaggctg ctgagtttaa
     3181 aaagcaacaa ttaaatgaac aaggtatttt caagaaacca gtgattacac ctattaaacc
     3241 atataaaaat ttctatccag ctgaagacta ccatcaagat tattacaaaa agaacccggt
     3301 acattattac caatatcaac gtggttcagg tagaaaagcg tttatagaat cacattgggg
     3361 gaatcaaaat gcttaaaaaa gataaaagtg aactaacaga tatagaatat attgttacac
     3421 aagaaaacgg cactgaacca ccatttatga atgaatattg gaatcatttt gctaaaggaa
     3481 tttatgtaga taaaatttct ggtaaacctt tatttacatc tgaagaaaag tttcattctg
     3541 aatgtggatg gcctagcttt tccaaagcgc ttgatgacga tgaaattata gaattagtcg
     3601 acaaatcatt tggtatgttg agaactgaag tgcgttcaga agaatcaaat agtcatttag
     3661 gacatgtctt taatgatgga cctaaagaaa gtggcggctt aagatactgt atcaattccg
     3721 ctgcaattca atttattcca tatgaaaagt tagaagaatt gggttatggc gatttaatat
     3781 cacattttga taagtaggag tggaaagtat gtttaaaaaa ttattcggaa aaggcaaaga
     3841 agttcagaaa gatatcgcaa tttatgcacc attaactgga gaattcgtga aaattgaaga
     3901 tattccagat cctgtattcg cacaaaaaat gatgggcgaa ggttttggta ttaatccaac
     3961 tgaaggagaa gttgtgtctc caattgcagg acgtgttgac aatgtctttc caactaagca
     4021 tgcgattggg ttaaaagcag ataacggatt agaattatta gttcatatcg gtttagacac
     4081 agttcaatta gacggtgaag gatttgaagt gttagtgtct agtggtgacg aagttaacgt
     4141 cggagatcca ttagtaagat tcaaccttga atatattaat aataacgcta aatctgtaat
     4201 ttcaccaatt ataattacta acactgatca agcagcttca attaatattt atgatgaaaa
     4261 tgctgtgatt aaaggtgaaa caaaagtgat tgatgtgaca atgaactaat gaaaaactat
     4321 tcgttttatc aatttgtcat gacagttcgt ggtcgacacg acgataaagg tcgtctagca
     4381 gaagagatat ttgacgatct tgctttccca aaacacgatg atgattttaa catactgtct
     4441 gattatattg agacacatgg tgatttcaca ttgccaatgt ctgtatttga tgatttatat
     4501 gaagaatata cggaatggct aaaattttaa tataattttt aataataata gttagaacca
     4561 gggtgatgca attcgttatc ctggttttaa tttaaaataa actaagttgt gactaaaaat
     4621 taatcaatta tagtgaaata tggtgcgcta tcttgcataa attgatatga ttaactacac
     4681 agaatttaaa agtacataat acataataag gaagtgatac aatggatgat aagcaacaca
     4741 catcttcatc cgatgatgaa cgcgctgaaa ttgcaacaag caatcaagac caagaaacta
     4801 attcatcgaa acgcgttcac ttaaaacgtt ggcaattcat atcaatatta attggtacaa
     4861 tcctaataac agctgtcatc acagttgttg catatatttt tataaatcaa aaaataagtg
     4921 gtttaaacaa aactgatcaa gcaaacttaa ataaaattga aaatgtgtat aaaatcttaa
     4981 atagtgatta ttacaaaaaa caggactctg acaagttaag taaagctgca attgatggca
     5041 tggtcaaaga attaaaagat ccttattctg aatatttaac aaaagaacaa acgaaatcct
     5101 ttaatgaagg tgtttcaggt gattttgtag gtattggtgc agaaatgcaa aagaaaaatg
     5161 atcaaattat ggttactagt cctatgaagg gatctccagc agaacgtgct ggcattcgtc
     5221 ctaaagatgt cattactaaa gtaaatggaa aatcaattaa aggtaaagca ttagatgaag
     5281 ttgtcaaaga tgttcgtggt aaagaaaaca ctgaagtcac tttaactgtt caacgaggta
     5341 gtgaagaaaa agacgttaag attaaacgtg aaaaaattca tgttaaaagt gttgagtata
     5401 agaaaaaagg taaagttgga gttattacta ttaataaatt ccagaatgat acatcaggtg
     5461 aattgaaaga tgcagttcta aaagctcaca aagatggttt gaaaaagatt gttttagatt
     5521 taagaaataa tccaggtgga ctactagatg aagctgttaa aatggcaaat atttttatcg
     5581 ataaaggaaa aactgttgtt aaactagaaa aaggtaaaga tactgaagca attcaaactt
     5641 ctaatgatgc gttaaaagaa gcgaaagaca tggatatatc catcttagtg aatgaaggtt
     5701 cagctagcgc ttctgaagtg tttactggtg cgctaaaaga ctataataaa gctaaagttt
     5761 atgggtcaaa aacattcggc aaaggtgtcg tacaaactac aagagagttt aaggatggtt
     5821 cattgttaaa atatactgaa atgaaatggt taacgccaga tggtcattat attcacggta
     5881 aaggcattaa accagacgtt actattgaca cacctaaata tcaatcttta aatgtcattc
     5941 ctaatacgaa aacatttaaa gttggagacg atgataaaaa tattaaaact attaaaattg
     6001 gtttatcagc tttaggttat aaagttgata atgaatcaac gcaatttgat aaagctttag
     6061 aaaatcaagt taaagctttc caacaagcga ataaacttga ggtaactggt gaatttaata
     6121 aagaaacgaa taataaattt actgagttat tagttgaaaa agctaataaa catgatgatg
     6181 ttctcgataa gttgattaat attttaaaat aagcgataca cactactaaa attgtattat
     6241 tattatgtta atgacacgcc tcctaaattt gcaaagatag caatttagga ggcgtgttta
     6301 tttttattga cgtctaactc taaaagatat aaattagaca tttacaaatg atgtaaataa
     6361 cgcaatttct atcatcgctg ataacaattc atggtttaat atgcaatgag catatacttt
     6421 ttaaatagta ttattcacta gttttaacaa tcaattaatt ggtatatgat acttttattg
     6481 gttattttta tcccatagtg tgataattac tatttttcat tcataataaa ggtttaaagc
     6541 atgttaatag tgtgtaagat taacatgtac tgaaaaacat gtttaacata atgatataag
     6601 gagtgacgta catgatccgt ctaggtaaaa tgtcagattt agatcaaatc ttaaatctag
     6661 tagaagaagc aaaagaatta atgaaagaac acgacaacga gcaatgggac gatcagtacc
     6721 cacttttaga acattttgaa gaagatattg ctaaagatta tttgtacgta ttagaggaaa
     6781 atgacaaaat ttatggcttt attgttgtcg accaagacca agcagaatgg tatgatgaca
     6841 ttgactggcc agtaaataga gaaggcgcct ttgttattca tcgattaact ggttcgaaag
     6901 aatataaagg agctgctaca gaattattca attatgttat tgatgtagtt aaagcacgtg
     6961 gtgcagaagt tattttaacg gacacctttg cgttaaacaa acctgcacaa ggtttatttg
     7021 ccaaatttgg atttcataag gtcggtgaac aattaatgga atatccgcca tatgataaag
     7081 gtgaaccatt ttatgcatat tataaaaatt taaaagaata gaggtaatat taatgacgaa
     7141 aatcgcattt accggagggg gaacagttgg acacgtatca gtaaatttaa gtttaattcc
     7201 aactgcatta tcacaaggtt atgaagcgct ttatattggt tctaaaaatg gtattgaaag
     7261 agaaatgatt gaatcacaac taccagaaat taagtattat cctatttcga gtggtaaatt
     7321 aagaagatat atttctttag aaaatgccaa agacgtattt aaagtattga aaggtattct
     7381 tgatgctcgt aaagttttga aaaaagaaaa acctgatcta ttattttcaa aaggtggatt
     7441 tgtatctgtg cctgttgtta ttgcagccaa atcattaaat ataccaactc ttattcatga
     7501 atctgactta acaccaggat tagcgaataa gatagcactt aaatttgcca agaaaatata
     7561 tacaacattt gaagaaacgc taaactactt acctaaagag aaagctgatt ttattggagc
     7621 aacaattcga gaagatttaa aaaatggtaa tgcacataat ggttatcaat taacaggctt
     7681 taatgaaaat aaaaaagttt tactcgttat gggtggaagc ttaggaagta aaaaattaaa
     7741 tagcattatt cgcgaaaact tagatgcatt attacaacaa tatcaagtga tacatttaac
     7801 tggtaaagga ttaaaagatg ctcaagttaa aaaatcagga tatatacaat atgaatttgt
     7861 taaagaggat ttaacagatt tattagcaat tacggataca gtaataagta gagctggatc
     7921 aaatgcgatt tatgagttct taacattacg tataccaatg ttattagtac cattaggttt
     7981 agatcaatcc cgaggcgacc aaattgacaa tgcaaatcat tttgctgata aaggatatgc
     8041 taaagcgatt gatgaagaac aattaacagc acaaatttta ttacaagaac taaatgaaat
     8101 ggaacaggaa agaactcgaa ttatcaataa tatgaaatcg tatgaacaaa gttatacgaa
     8161 agaagcttta tttgataaga tgattaaaga cgcattgaat taatgggggg taatgcttta
     8221 tgagtcaatg gaaacgtatc tctttgctca tcgtttttac attggttttt ggaattatcg
     8281 cgtttttcca cgaatcaaga cttgggaaat ggattgataa tgaagtttat gagtttgtat
     8341 attcatcaga gagctttatt acgacatcta tcatgcttgg ggctactaaa gtaggtgaag
     8401 tctgggcaat gttatgtatt tcattacttc ttgtggcata tctcatgtta aagcgccaca
     8461 aaattgaagc attatttttt gcattaacaa tggcattatc tggaattttg aatccagcat
     8521 taaaaaatat attcgataga gaaagaccta cattgctgcg tttaattgat ataacaggat
     8581 ttagttttcc tagcggtcat gctatgggat caactgcata ttttggaagt ggtatctatc
     8641 tattaaatcg attaaatcaa ggtaattcaa aaggtattct tatagggtta tgtgcagcta
     8701 tgattttatt gatttccata tcacgtgtat atctaggtgt acattatcca acagatatta
     8761 ttgccggcat tattggtgga ttattttgca ttattttatc aacgttatta cttagaaata
     8821 aattaataaa ttaaatagta aaat
//
LOCUS       DALAGF010000109         8834 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.109, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000109 DALAGF010000000
VERSION     DALAGF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8834
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.109"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            469..1428
                     /locus_tag="O2692_002302"
     CDS             469..1428
                     /locus_tag="O2692_002302"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCZ4299912.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            1706..2410
                     /gene="budA"
                     /locus_tag="O2692_002303"
     CDS             1706..2410
                     /gene="budA"
                     /locus_tag="O2692_002303"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCZ4299913.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(2496..3356)
                     /locus_tag="O2692_002304"
     CDS             complement(2496..3356)
                     /locus_tag="O2692_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCZ4299914.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HISFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIAHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            3429..4247
                     /gene="panB"
                     /locus_tag="O2692_002305"
     CDS             3429..4247
                     /gene="panB"
                     /locus_tag="O2692_002305"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCZ4299915.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            4240..5091
                     /locus_tag="O2692_002306"
     CDS             4240..5091
                     /locus_tag="O2692_002306"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCZ4299916.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITIVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            5093..5476
                     /locus_tag="O2692_002307"
     CDS             5093..5476
                     /locus_tag="O2692_002307"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCZ4299917.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(5605..7287)
                     /locus_tag="O2692_002308"
     CDS             complement(5605..7287)
                     /locus_tag="O2692_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HCZ4299918.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGTIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKQQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            7344..7442
                     /locus_tag="O2692_002309"
     CDS             7344..7442
                     /locus_tag="O2692_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299919.1"
                     /translation="MPIINVGDLWDNEHNVYNHWGGLDINDYRSTY"
     gene            7420..8007
                     /locus_tag="O2692_002310"
     CDS             7420..8007
                     /locus_tag="O2692_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCZ4299920.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(8088..8537)
                     /locus_tag="O2692_002311"
     CDS             complement(8088..8537)
                     /locus_tag="O2692_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HCZ4299921.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
BASE COUNT         2952 a         1369 c         1691 g         2822 t
ORIGIN      
        1 ctaacaattt tgcacattat atttttaaaa tcaatttcat gtttatgtct gattggctag
       61 tttgtatgaa atgtaattct ttgactataa aatgatgcat caaatatgat gagggtaagg
      121 cggaaaatag gaggttatat agtaagtcat ttaagtcgaa tagccatcct ttttaaaatg
      181 aaaaaaatag aaagctcaat agtttgttaa agcctttaaa tagcgtcgtt acgcaatttt
      241 agaatgctaa aaattgtcac aaatttcaaa ttacgtgcta aagcttgaga tatcaatatt
      301 tattggcgat agagtgtaat ttgactccgc ttacatctca gttttatgtt tgtaatttgg
      361 tagcataata ttataataaa ataaaattgt taatctttaa tttcagtata gatattttta
      421 cgtgtagtca cgtgtaaaat aaattcaatt aggttaggag acataattat gaaaacattt
      481 ggtaaaaagg ttgtattaat cggagatgga tctgtaggat caagctatgc ctttgcaatg
      541 gttacgcaag gtgttgctga tgaatttgta attattgaca ttgcaaaaga caaagtaaaa
      601 gcagatgttc aagatttaaa ccatggtaca gtccacagtc cttcaccagt tgatgtgaaa
      661 gcaggtgaat acgaagactg taaagatgca gatttagttg ttattacagc tggtgcacct
      721 caaaagccag gtgaaacacg tttacaatta gttgaaaaaa atactaagat tatgaagagc
      781 atcgttaaga gtgttatgga tagtggcttt gatggatatt tcttaatcgc ggcaaaccct
      841 gtagacattt taacaagatt tgtaaaagaa tatactggat taccagcaga gcgtgttatc
      901 ggttcaggta ctgtattgga cagtgcacgt ttacaatatt taattagcca agaacttggt
      961 gttgcacctt caagtgttga cgctagtatt attggcgagc atggtgatac tgaacttgca
     1021 gtttggtcac aagcaaatgt agcaggtatt tcagtatatg acacattaaa agaacaaact
     1081 ggtagcgaag ctaaagcgga agaaatttat gtgaatacac gtgacgctgc ttatgaaatt
     1141 atccaagcta aagggtcaac atactatggt attgcattag cattgatgcg catttcaaaa
     1201 gccattttaa ataatgaaaa taatgtctta aatgtttcta tacaattaga tggtcaatat
     1261 ggtggtcaca aaggcgttta cctaggtgta ccaacattag ttaaccaaca tggcgcagtt
     1321 aaaatttatg aaatgccatt aagtgccgaa gaacaagcgt tgttcgataa atctgttaaa
     1381 acattagaag atacatttga ttcaattaaa tatttattag aagactaagc ctattttaag
     1441 tattaattag aaatatttta atggtaagag gatctattag tgttgcagta acacgtggca
     1501 ctgataggtc cattttttta gcaagcattg atagaggcaa tacttttgcg taaaataata
     1561 attgaataaa gtattatgac tcgtgaaaat ttagtgacat gaataaaaat gttgaggcgt
     1621 cattgagtat aaaggaaagt agttctgcat taatcacgaa gtagagcatg acaacgagga
     1681 ataactatag ggagatggtt ttggaatgac gaatgtcttg tatcaacatg gtacattagg
     1741 tacgttaatg gctggcttac tagaaggcac agctacaatt aatgaattgt tagaacatgg
     1801 gaatttaggg attgcaacgt taacagggtc tgatggcgaa gtaatatttt tagacggaaa
     1861 ggcatatcat gctaacgagc ataaagaatt tatagaatta aaaggcgatg agaaagtacc
     1921 gtatgcatcg attactaatt ttaaagcgag taagacattt ccattgcaac aattatcaca
     1981 agatgatgta tttgcacaaa ttaaaaatga aatgttaagt gagaatttat tttcggctgt
     2041 taaaatttat ggcacattta aacatatgca tgtacgaatg atgcctgctc agcaaccgcc
     2101 atatacacgt ttgattgatt cagcacgcag acaacctgag gaaaaaagac aagatattcg
     2161 tggtgccatt gttggatttt ttacaccaga attatttcat ggcgtagggt ctgctggttt
     2221 tcatatacat tttgcggatg atgaaagagc ttatggtgga catgttcttg actttgaagt
     2281 ggatgacgtt gtcgttgaga tacaaaactt tgaaacattc caacaacatt tcccggtaaa
     2341 taacgagacg tttgttaaag cgaaaataga ctataaagat gtggcagaag aaattagaga
     2401 agctgaatag tctaatataa ttaaaagacc ttagcgatat taggaacagg tggttctaaa
     2461 tgcatcgtta aggtcttttt atattatata tgtgcttaca tattttgttg atacgcgcgt
     2521 aaaaagctat aaatagtatc aagatatggg gtgtcaagat tgtgttcacg tgcacgtcga
     2581 taaataaagc cttgaatcgc ttcgacttca agaggttgct gatgcgcaat gtcgtaatac
     2641 atgctcgttc ccatttcgtc gggataacct tgatatatag tcataatagt atcgacagtt
     2701 tgttctgaaa agtttaaccc ctctgcctgt gcaacacgac aaccatctag taatagctgt
     2761 ctacacagtg tacgtatttc aggattatgc atgattgcaa cggtctgtct accgagtgct
     2821 gtgatagaat taatgcccaa gttaactaac aatttatacc aaatagcttg ttgaatattt
     2881 gcttctaata caatgtcgat ttgactgtct tggaccaaat ctctaaattg tcgagttaat
     2941 gcattatctt gtatacgtaa ttgataatct ctgaagtgcg taacaacatc gcctttcttt
     3001 tgaccactta tatagacaac tgcttggcat acgtttttaa atgaaatatg ttcgagttga
     3061 ccataaccat tttgggctaa aatgatgagc gtgtcctcat gtgcaagatg agttaaatga
     3121 ggaataactg catcaagttg atgtgttttg actgcaataa taataacatc aaatgtgttt
     3181 gtgacatctt cataaccttt cacaacaata tcttgtgcag gtgcatgtgg tacagtataa
     3241 tatgttattg ttttggcgtg tctcccgata agcgttgtat gaggcaatga ttgttgtaat
     3301 tcataggcaa tagttgtacc gacagcgcct gggccgataa tcgcaactga taacatcttc
     3361 ttctttcctt tctataaaag attcattata ctatttctaa taatactgca aaaagaggtt
     3421 gaataacatt gaaaacagta agtcaattaa tagatatgaa acaaaagcaa actaaaattt
     3481 ctatggtaac agcttatgat tttccaagtg ctaaacaagt ggaagcagcg ggtattgata
     3541 tgattcttgt tggggattca cttggtatga ctgtattagg ttatgaaagt accgtacaag
     3601 tgacgttagc agatatgatt catcacggtc gtgcagtaag aagaggcgca ccaaatacat
     3661 ttgtagttgt tgatatgccg attggtgcag taggcatctc tatgacacaa gacttaaatc
     3721 atgctctaaa gctttatcag gagacgaatg caaatgcaat taaagcagaa ggagcgcata
     3781 ttacaccatt tattgaaaaa gcaacggcaa ttggtattcc tgttgttgca catttaggat
     3841 taacacctca aagtgttggc gttatggggt ataaattaca aggtgcaacg aaagaagcag
     3901 cggagcaact tattttagac gcgaaaaatg tagaacaagc tggtgcagtt gcgttagtac
     3961 ttgaagcaat tccaaatgat ttagcagaag aaattagcaa gcacttaaca attccagtca
     4021 ttggtattgg tgcaggaaaa ggtacagatg gtcaagtatt ggtttatcac gatatgttaa
     4081 attatggtgt tgaacataaa gcgaaatttg tgaagcaatt tgctgatttt agtgttggtg
     4141 tggatggttt aaaacaatac gatcaagaag ttaaatctgg tgcatttcca tcagaagaat
     4201 atacgtataa aaagaaaatt atgaatgagg ttaataacaa tgactaagct gattactacg
     4261 gtaaaagaga tgcaacacat tgttaaagca gccaaacgct caggcacaac gattggtttt
     4321 atcccaacaa tgggtgcctt acatgatgga catttaacga tggtacgcga atcagttagt
     4381 acaaatgata ttacaattgt gagtgtattt gttaatccat tacaatttgg tccaaacgaa
     4441 gatttcgatg cttatccgag acaaattgat aaagatttag aattagtatc agaagtagga
     4501 gcggacattg tttttcatcc tgcagtagaa gatatgtatc caggtgaatt aggtattgat
     4561 gtcaaagtag gcccattagc tgatgtgtta gaaggagcga agcgtccagg gcattttgat
     4621 ggggtggtaa cagtagttaa taagctattt aatattgtta tgccggatta tgcttatttt
     4681 ggtaaaaaag atgcccagca attggctatt gtcgagcaaa tggtaaaaga cttcaatcat
     4741 gccgttgaaa ttattggtat tgatatcgtt cgagaagcag atggtttggc gaaaagttca
     4801 agaaatgttt atttaacgga gcaagaacga caagaagcgg tacatttaag taaaagtttg
     4861 ctattagcac aagcgttgta tcaagacggt gaacgtcaaa gtaaagtaat tatagacagg
     4921 gttactgaat atcttgaatc acatataagt ggacgcattg aagaagttgc tgtttatagt
     4981 tatcctcaat tagtagaaca acacgaaata actggacgca tatttatctc gttagctgtt
     5041 aaattttcaa aagcgcgttt aatagataat ataataattg gagctgaata aagtgataag
     5101 aacaatgatg aatgctaaaa ttcatagagc aagagtgact gagtcaaatt taaattatgt
     5161 aggtagcatt acgattgatt cagatatatt ggaagcggta gacatcttgc caaatgaaaa
     5221 agtagccatc gtgaataata ataatggtgc acgttttgaa acatacgtaa tagctggtga
     5281 aagaggtagt ggtaaaattt gtttaaacgg tgcagcgtca agacttgttg aagtaggcga
     5341 tgtagtcata atcatgactt atgcacagtt gaatgaagaa gaaattaaaa accatgcacc
     5401 taaagtagca gtaatgaatg aagataatgt cattattgaa atgattcatg aaaaagaaaa
     5461 tacgatagtg ttataaaatt aaatagccat caataataaa aagggccaca ccttggttta
     5521 agattccaag cgtgtggctt tctcttgtta tacagtccta tcactatgtc acttttaatc
     5581 aaaagcgacc gtaattgctg catatcaatt aacgatagga atgattaatt gtgaatcata
     5641 tttaccacca gtataaatca tgtgcatgcc tttatttacg gtttcttcat gttcataacg
     5701 tgttttcatg ccaggagtac tattaccaat tacaatttca ctacccttta caacaacttc
     5761 caatgtttcg ccttgtttaa atagcgtgcc tgaaggtaac aattcgattt caacaggtac
     5821 aatctcatct tgtgacaact ttaattctgt ttcatgttta tgccaaggtt gcgcgattga
     5881 ggatttttct tgatctaatt cacgatgtga tacgcgtaac caaccagtag ctacttgacc
     5941 attttcaata tgattaaaat cagggaagtt aacttcatta ccacgacgat ctaacttttt
     6001 aatacctgca aataaatcca tatcatctga gtctttagtg cttacccata gttttaagtt
     6061 catgtttcca actaactcag tatctttgtc aaacgtatat ttaaaactta catcttgttg
     6121 tttatcttca gagtcatatt gtgcgacatg cgcgctacta atctttgcat gattcaatgt
     6181 gtgattttca gcattcaaat acaatggtgt atattctgcg ttaggtaaag ggaacgctga
     6241 cgctgatttg aattcgcctt tataaaattg atctctaact tcataaatga catgaggcgt
     6301 atctttccaa tcgttatttt cttctttaag gtaaaaatca aagaatgatt tttggcgttc
     6361 gagattttct ctagcgtagt aactttccca ctctttacgt ccatgcacat atagccattt
     6421 ttcttcagat gcagcttgtt taaatccttc aaaagagcca cggttgtgca aaccttgtgt
     6481 agaccaacta gcacatgtta gtagaggtgt tttaatttgt gataatggca cttgacgctg
     6541 tttccaaaaa tcatcgaaca gaggatgttc ttgttgtgct tgaatcaaat cttcgatatt
     6601 tggattatct gtccatctcg caaaaatacc ttgagtccag aaacgataaa agccagtatc
     6661 tggtatacct ccgtgaaagg ctacttctct atacatatca tttaagcctt cccaaggaat
     6721 cattgctttt aaatgtggtg gatttaatga tgcgacccac cattgagtca ccgcaagata
     6781 agaaacacca tttgtcccga tatttccatt actccatgac tgatttgctg cccattcaat
     6841 cacttcgtaa taatcttccg cttctctttt tgaccatgga gataagacgc ctttggattt
     6901 gtcactaccg cgtaatgcaa ctttaactac aacataatca tttggcaccc aaaatcctgg
     6961 gtctggtgat tcttcaggtg taaaactaga tgtcggaatg gtacctaatg ttggccaaag
     7021 ggcacccata tttgtgattt taggcttatt atctttaccg taagtatctg cagacataac
     7081 tacagggaat ttgccatctt tatttggtct gaaaatatta atatataatt tttcgccatc
     7141 gcgcatttcc acagtgacat ctttttccat aatcatttct tgatttccat attgaacact
     7201 gtcgacaacg atgtggttaa taccggcttt aacttcattg acatgagtta cagttaattt
     7261 tggatttcct agtaaatgtt ggttcattgt tatcaccttt ttctgtttgt ttgtatcacc
     7321 tatattaaac aggtaaacat tgtgtgccaa tcatcaatgt tggagatttg tgggataatg
     7381 aacataacgt ttataatcat tggggaggac ttgatataaa tgactataga tcgacgtatt
     7441 agaaaatcac aagttgcaat gagaaatgca tttattgaac ttttacatca acaccaatta
     7501 gaagagatta ctgtgcaaca aattgcagat ttagctgatg ttaaccgtag tacattttat
     7561 acgcattatt atgataagta tgatttgctt gaaaaattag aggaccagca gttggaggaa
     7621 ataagagact tcattcatga tgagaagtta aatggagcag ttaagttatc cacggataac
     7681 atacatcaaa ttatgaccgt tttaatagaa atgatagaaa aagacatcgc attttatcaa
     7741 ttgatgtttc gtatgggtaa agattcaaat atccacgaaa aactttatga tttaattatg
     7801 tgtcatttac aacgttataa aaatgaaaat gatcaaatta atggtatacc attttcttat
     7861 ttcatgagtt atgtttcagg tgcgggattg tcttttataa gacattgggt agaagatcca
     7921 aaccgtatag acaaagttga cttaataagg cattttaata cgattgtcaa tcaagggcca
     7981 gcaacgataa ttaaaaatat acaataaaat aaggcaagcc atgaatcgag agtggcttgc
     8041 cttgctaaat aacataagct tattaaatga tatctttatc aataacatta ttgttggatt
     8101 gcagtatagt cttgaccatg taacatccaa cgaacaccgt atttatcggt aaagacgccc
     8161 attttgccac cccagaattg gtcagcaaac ggtaattcta tttcaattga agaatgatct
     8221 ttaatttgct catagaatgc ttcaacttta tcagcatctt ccttattgtt aacatcataa
     8281 tcaattaata atgatatgcc attattaatt ttgtcagcgc gaccaaaaga atcagaacat
     8341 aacactttta cgccaagcac ttcaaattca gcatgcatag ttgcttcttg cgcttcttcc
     8401 ttagtcatac caaaatgtga cgcttgttct tcgccaactt ctaaacgttt aacgtcagtt
     8461 gcaccaaata cttcttcgta atatgcaagg gcttcttttg aattttcaaa agcgatataa
     8521 gggaataatg ctgtcatgta aaacacctca atgtattatt tttattacta acaccatgtt
     8581 acattaaaaa atggcaaaag tataactttt acgatgaatt gaagtcaatc ttttgtaaaa
     8641 tacacgataa attataagat gaaattgatt taaatgaagt aaacatatat atgacataca
     8701 aaaaatgaaa atgacacaga gacgtatgtg ttgtttagtg ttttaaaatt caagtttaaa
     8761 aaatgctttt agatacataa gggattttcg aaatataaat gcattaaaat gaatttctaa
     8821 cttttttgac tgac
//
LOCUS       DALAGF010000110         8720 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.110, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000110 DALAGF010000000
VERSION     DALAGF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8720
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.110"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            162..317
                     /locus_tag="O2692_002312"
     CDS             162..317
                     /locus_tag="O2692_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299922.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            325..846
                     /locus_tag="O2692_002313"
                     /pseudo
     CDS             325..846
                     /locus_tag="O2692_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..1515
                     /locus_tag="O2692_002314"
     CDS             985..1515
                     /locus_tag="O2692_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCZ4299923.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNTLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            1778..4561
                     /gene="clfA"
                     /locus_tag="O2692_002315"
     CDS             1778..4561
                     /gene="clfA"
                     /locus_tag="O2692_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HCZ4299924.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSS
                     STNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSL
                     AAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLT
                     MPAYIDPENVKKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFV
                     NPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRST
                     LYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSD
                     NDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSESDSDSESDSDSDSDSDSDSDSDSDSDSDSASDSDSGSDSDSSSDSDSESDSNSD
                     SESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLLASIGSL
                     LLFRRKKENKDKK"
     gene            4782..6308
                     /gene="vwb"
                     /locus_tag="O2692_002316"
     CDS             4782..6308
                     /gene="vwb"
                     /locus_tag="O2692_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor binding protein Vwb"
                     /protein_id="HCZ4299925.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTN
                     NKNKSRTVEEYKKSLDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFS
                     EEKKIKNGNTDNLDYLGLSHERYESVFNTLKKQSEEFLKEIEDIKKDNPELKDFNEEE
                     QLKCDLELNKLENQILMLGKTFYQNYRDDVESLYSKLDLIMGYKDEERANKKAVNKRM
                     LENKKEDLETIIDEFFSDIDKTRPNNIPVLEDEKQEEKNHKNMAQLKSDTEAAKSDES
                     KRSKRSKRSLNTQNHKPASQEVSEQQKAEYDKRAEERKARFLDNQKIKKTPVVSLEYD
                     FEHKQRIDNENDKKLVVSAPTKKPTSPTTYTETTTQVPMPTVERQTQQQIIYNAPKQL
                     AGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALPGRKSGSLVGISQIDSSHLTER
                     EKRVIKREHVREAQKLVDNYKDTHSYKDRINAQQKVNTLSEGHQKRFNKQINKVYNGK
                     "
     gene            6659..7681
                     /gene="emp"
                     /locus_tag="O2692_002317"
     CDS             6659..7681
                     /gene="emp"
                     /locus_tag="O2692_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HCZ4299926.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKKFVVPESGIN
                     KIIPAYDEFKNSPKVNVSNLTDNKNFVASEDKLNKIADSSAASKIVDKNFVVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
     gene            8016..8486
                     /locus_tag="O2692_002318"
     CDS             8016..8486
                     /locus_tag="O2692_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299927.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVTESGANDTKQFTEIVS
                     EEKVITVEHAQINIFQSNSNSNLMEFNILTMGGKSGAMVGYSEIDSSHFTDRDKRVIR
                     RDHVKEAQSLVENYKDTQSADARMKAKQKVNTLSKPHQNYFNKQIDKVYNGLQR"
BASE COUNT         3376 a         1283 c         1556 g         2505 t
ORIGIN      
        1 cgtttgtaaa aataaaaaaa tcaacttatt tgtatgagat aaatatgtat tgaagaagac
       61 gtgttattaa tttggaaaat actggtcaaa gatgggagct cttaaaagcg ttattgtatt
      121 ttttagtcaa tacaaataga ttgccgtaat aataatcgta cttgatggtt aaaaaattac
      181 ttaaggctat aaagcaaaac tttttatatg agcagtcgaa tataacgttt aaaatgattg
      241 tttttggata taaacgatta agtaaaatgc tttttcagtt tgaaattaat catataaatt
      301 tcttatggga gggttgatat cttaatgatt aacattattt cagctatagg atctattgga
      361 acatttatta tggctttatt ttattttgta tcagtttcag ttcaacttta tcaaatgaaa
      421 attagctttc tgccagcttt aggttttaac caaattttat tagaaaggga ggaggatcaa
      481 cttaatataa tgaattcggc aacagaagag catcatcata aagattatat taaactatat
      541 aatttaggtg gcggtgctgc taaaaaaatt gcaatagagg ttttattggg gaaggataaa
      601 gtcattcaga aaaaatacgt gcatatttta cctagtaaag aagggtacat gttaccaatt
      661 aataaaaatg tgtacgaaga attagaaaga acgattgaga acaatggtca tgaagctgat
      721 ttgaatgtac gtatgactta ttatcataat gtaagtcgca aacaatagga agttatatta
      781 aaaggtcaaa tcgaccgttt taatacttat aataataaag aaatttatga tttgcagttt
      841 atctaaaaat tgatttaaga gggtagttgt ttattgcgaa aaatatcatt caattttaat
      901 gaaataatgg cgtcattact ataaaatatt actttatgtt gtaatgcatt tttctataag
      961 atagaactaa aaggaggggc aaagatgcaa attagacaaa tacatcaaca tgactttgct
     1021 caagtggacc agttaattag aacggcattt gaaaatagtg aacatggtta tggtaatgaa
     1081 tcagagctag tagaccaaat tcgtctaagt gatacgtatg acaatacctt agaattagta
     1141 gctgttcttc aaaatgaagt tgtagggcac ggtttactaa gtgaagttta tcttgataac
     1201 gaggcacaac gggaaattgg attagtgtta gcacctgtat ctgttgatat tcatcatcaa
     1261 aataaaggta ttgggaagcg attgattcaa gcattagaac gagaagcaat attaaaagga
     1321 tataatttta tcagtgtatt aggatggccg acgtattatg ccaatctagg atatcaacgc
     1381 gcaagtatgt acgacattta tccaccatat gatggtatac cagacgaagc gtttttaatt
     1441 aaagaattaa aagtgaacag tttagcggga aaaacaggta ccataaatta cacatctgct
     1501 tttgaaaaaa tatgatttca agctaggatt acattaggta gagttcatat taataataaa
     1561 aaatgtttgc aatcaaatcg tacgttgtcg tttgtaattc ttaaaatagc aataaataaa
     1621 atgtttgtta gtaaagtatt attgtggata ataaaatatc gatacaaatt aattgctata
     1681 atgcaatttt agtgtataat tccattaaca gagattaaat atatctttaa agggtatata
     1741 gttaatataa aatgactttt taaaaagagg gaataaaatg aatatgaaga aaaaagaaaa
     1801 acacgcaatt cggaaaaaat cgattggcgt ggcttcagtg cttgtaggta cgttaatcgg
     1861 ttttggacta ctcagcagta aagaagcaga tgcaagtgaa aatagtgtta cgcaatctga
     1921 tagcgcaagt aacgaaagca aaagtaatga ttcaagtagc gttagtgctg cacctaaaac
     1981 agacgacaca aacgtgagtg atactaaaac atcgtcaaac actaataatg gcgaaacgag
     2041 tgtggcgcaa aatccagcac aacaggaaac gacacaatca tcatcaacaa atgcaactac
     2101 ggaagaaacg ccggtaactg gtgaagctac tactacgaca acgaatcaag ctaatacacc
     2161 ggcaacaact caatcaagca atacaaatgc ggaggaatta gtgaatcaaa caagtaatga
     2221 aacgacttct aatgatacta atacagtatc atctgtaaat tcacctcaaa attctacaaa
     2281 tgcggaaaat gtttcaacaa cgcaagatac ttcaactgaa gcaacacctt caaacaatga
     2341 atcagctcca cagagtacag atgcaagtaa taaagatgta gttaatcaag cggttaatac
     2401 aagtgcgcct agaatgagag catttagttt agcggcagta gctgcagatg caccggcagc
     2461 tggcacagat attacgaatc agttgacgaa tgtgacagtt ggtattgact ctggtacgac
     2521 tgtgtatccg caccaagcag gttatgtcaa actgaattat ggtttttcag tgcctaattc
     2581 tgctgttaaa ggtgacacat tcaaaataac tgtacctaaa gaattaaact taaatggtgt
     2641 aacttcaact gctaaagtgc caccaattat ggctggagat caagtattgg caaatggtgt
     2701 aatcgatagt gatggtaatg ttatttatac atttacagac tatgtaaata ctaaagatga
     2761 tgtaaaagca actttgacca tgcccgctta tattgaccct gaaaatgtta aaaagacagg
     2821 taatgtgaca ttggctactg gcataggtag tacaacagca aacaaaacag tattagtaga
     2881 ttatgaaaaa tatggtaagt tttataactt atctattaaa ggtacaattg accaaatcga
     2941 taaaacaaat aatacgtatc gtcagacaat ttatgtcaat ccaagtggag ataacgttat
     3001 tgcgccggtt ttaacaggta atttaaaacc aaatacggat agtaatgcat taatagatca
     3061 gcaaaataca agtattaaag tatataaagt agataatgca gctgatttat ctgaaagtta
     3121 ctttgtgaat ccagaaaact ttgaggatgt cactaatagt gtgaatatta cattcccaaa
     3181 tccaaatcaa tataaagtag agtttaatac gcctgatgat caaattacaa caccgtatat
     3241 agtagttgtt aatggtcata ttgatccgaa tagcaaaggt gatttagctt tacgttcaac
     3301 tttatatggg tataactcga atataatttg gcgctctatg tcatgggaca acgaagtagc
     3361 atttaataac ggatcaggtt ctggtgacgg tatcgataaa ccagttgttc ctgaacaacc
     3421 tgatgagcct ggtgaaattg aaccaattcc agaggattca gattctgacc caggttcaga
     3481 ttctggcagc gattctaatt cagatagcgg ttcagattcg ggtagtgatt ctacatcaga
     3541 tagtggttca gattcagcga gtgattcaga ttcagcaagt gattcagact cagcgagtga
     3601 ttcagattca gcaagcgatt ccgactcagc gagcgattcc gactcagaca atgactcgga
     3661 ttcagatagc gattctgact cagacagtga ctcagattcc gacagtgact cagattcaga
     3721 tagcgattct gactcagaca gtgactcaga ttcagatagc gattcagatt cagatagcga
     3781 ttcagattcc gacagtgatt ccgactcaga cagcgattct gactccgaca gtgattccga
     3841 ctcagacagc gattcagatt ccgacagtga ttccgactca gatagcgatt ccgactcaga
     3901 tagcgactca gattcagaca gcgattcaga ttcagacagc gattcagatt ccgacagtga
     3961 ctcagattcc gacagtgact cggattcaga tagcgattca gattccgaca gtgactcaga
     4021 ttccgacagt gactcagact cagacagtga ttcggattca gcgagtgatt cggattcaga
     4081 tagtgattcc gactccgaca gtgactcgga ttcagatagc gactcagact cggatagcga
     4141 ctcggattca gatagcgatt cggactcaga tagcgattca gaatcagaca gcgattcaga
     4201 atcagacagc gattcagatt cagacagcga ctcagacagt gactcagatt cagatagtga
     4261 ctcggattca gcgagtgatt cagactcagg tagtgactcc gattcatcaa gtgattccga
     4321 ctcagaaagt gattcaaata gcgattccga gtcaggttct aacaataatg tagttccgcc
     4381 taattcacct aaaaatggta ctaatgcttc taataaaaat gaggctaaag atagtaaaga
     4441 accattacca gatacaggtt ctgaagatga agcaaatacg tcactaattt ggggattatt
     4501 agcatcaata ggttcattac tacttttcag aagaaaaaaa gaaaataaag ataagaaata
     4561 agtaataatg atattaaatt aatcatatga ttcatgaaga agccacctta aaaggtggct
     4621 tttttacttg gattttccaa atatattgtt tgaatataat taataattaa ttcatcaaca
     4681 gttaattatt ttaaaaaggt agatgttata taatttggct tggcgaaaaa atagggtgta
     4741 aggtaggttg ttaattaggg aaaattaagg agaaaataca gttgaaaaat aaattgctag
     4801 ttttatcatt gggagcatta tgtgtatcac aaatttggga aagtaatcgt gcgagtgcag
     4861 tggtttctgg ggagaagaat ccatatgtat ctgagtcgtt gaaactgact aataataaaa
     4921 ataaatctag aacagtagaa gagtataaga aaagcttgga tgatttaata tggtcctttc
     4981 caaacttaga taatgaaaga tttgataatc ctgaatataa agaagctatg aaaaaatatc
     5041 aacagagatt tatggctgaa gatgaggctt tgaagaaatt ttttagtgaa gagaaaaaaa
     5101 taaaaaatgg aaatactgat aatttagatt atctaggatt atctcatgaa agatatgaaa
     5161 gtgtatttaa tactttgaaa aaacaaagtg aggagttctt aaaagaaatt gaagatataa
     5221 aaaaagataa ccctgaattg aaagacttta atgaagagga gcaattaaag tgcgacttag
     5281 aattaaacaa attagaaaat cagatattaa tgttaggtaa aacattttat caaaactata
     5341 gagatgatgt tgaaagttta tatagtaagt tagatttaat tatgggatat aaagatgaag
     5401 aaagagcaaa taaaaaagca gttaacaaaa ggatgttaga aaataaaaaa gaagacttag
     5461 aaaccataat tgatgaattt tttagtgata tagataaaac aagacctaat aatattcctg
     5521 ttttagaaga tgaaaaacaa gaagagaaaa atcataaaaa tatggctcaa ttaaaatctg
     5581 acactgaagc agcaaaaagt gatgaatcaa aaagaagcaa gagaagtaaa agaagtttaa
     5641 atactcaaaa tcacaaacct gcatctcaag aagtttctga acaacaaaaa gctgaatatg
     5701 ataaaagagc agaagaaaga aaagcgagat ttttggataa tcaaaaaatt aagaaaacac
     5761 ctgtagtgtc attagaatat gattttgagc ataaacaacg tattgacaac gaaaacgaca
     5821 agaaacttgt ggtttctgca ccaacaaaga aaccaacatc accgactaca tatactgaaa
     5881 caacgacaca ggtaccaatg cctacagttg agcgtcaaac tcagcaacaa attatttata
     5941 atgcaccaaa acaattggct ggattaaatg gtgaaagtca tgatttcaca acaacgcatc
     6001 aatcaccaac aacttcaaat cacacgcata ataatgttgt tgaatttgaa gaaacgtctg
     6061 ctttacctgg tagaaaatca ggatcactgg ttggtataag tcaaattgat tcttctcatc
     6121 taactgaacg tgagaagcgt gtaattaagc gtgaacacgt tagagaagct caaaagttag
     6181 ttgataatta taaagataca catagttata aagaccgaat aaatgcacaa caaaaagtaa
     6241 atactttaag tgaaggtcat caaaaacgtt ttaataaaca aatcaataaa gtatataatg
     6301 gcaaataatt aatgcatggc tgcaaagcaa ataatgagtt tgtcgtaaaa ataacaacat
     6361 tttaaactag caataaataa tatcaaagtc atcatttcaa tgatgcaatc tagtatagtc
     6421 cacattctaa acaggtgtgg actattactt ttttcacttt atattacgaa aaaattatta
     6481 tgcttaacta tcaatatcaa taattaattt taagctgaaa aacaataaaa atgttaagac
     6541 aacgtttact tcaagttaat tattatactg aaaattctgg tatataatgc tgttagtgaa
     6601 tataacaggg aaattatatt ggttataata ttgagtctat ataaaggaga aataacagat
     6661 gaaaaagaaa ttattagttt taactatgag cacgctattt gctacacaaa ttatgaattc
     6721 aaatcacgct aaagcatcag tgacagagag tgttgacaaa aaatttgtag ttccagaatc
     6781 aggaattaat aaaattattc cagcttacga tgaatttaag aattcgccaa aagtaaatgt
     6841 tagtaattta actgacaata aaaactttgt agcttctgaa gataaattga ataagattgc
     6901 agattcatcg gcagctagta aaattgtaga taaaaacttt gtcgtaccag aatcaaagtt
     6961 aggaaacatt gtgccagagt acaaagaaat caataatcgc gtgaatgtag caacaaacaa
     7021 tccagcttca caacaagttg ataagcattt tgttgctaaa ggcccagaag taaatagatt
     7081 tattacgcaa aacaaagtaa accaccactt cattactacg caaacccact acaagaaagt
     7141 tattacttca tacaaatcaa cacatgtaca taaacatgta aatcatgcaa aggattctat
     7201 taataaacac tttattgtta aaccatcaga atcgcctaga tatacacatc catctcaatc
     7261 tttaattatc aagcatcatt ttgcagttcc tggatatcac gcgcataaat ttgttacacc
     7321 agggcatgct agcattaaaa ttaatcactt ttgtgttgtg ccacaaataa atagtttcaa
     7381 ggtaattcca ccatatggtc acaattcaca tcgtatgcat gtaccaagtt tccaaaataa
     7441 cacaacagca acacatcaaa atgctaaagt aaataaagca tatgactata aatacttcta
     7501 ttcttataaa gtagttaaag gtgtgaagaa atatttctca ttttcacaat caaatggtta
     7561 taaaattggg aaaccatcat taaatatcaa aaatgtaaat tatcaatatg ctgttccaag
     7621 ttatagccct acacactacg ttcctgaatt taagggtagc ttaccagcac cacgagtata
     7681 aaaattggca ctaagtttac gagatatgat aaatacctat tattttaaat atagtctaca
     7741 atctatgtgg ttgtaggctg tattttttgc agtttatcaa taaacaccca tcaacaaatt
     7801 ataccgtttt tctactttga aagttggaag taacataatc ttaaataaat atattattaa
     7861 ttaagataaa tataatactc aagattattg ttaatagttt gttcatcgca agttaattat
     7921 tgtttctaaa atattggtat ataattttca atggcgaaga aaacagggtg aaaaagtcgg
     7981 tttttatatc aaagcaaata agggagcata aacaaatgaa aaggaaagta ttagtactaa
     8041 caatgggtgt aatttgtgca actcaattat ggcattctaa tcacgcaaac gcattagtaa
     8101 cagagagtgg cgcaaatgat actaagcaat ttactgaaat tgtatcggaa gagaaagtta
     8161 taacagttga acatgctcaa attaatattt ttcaatctaa tagcaattca aacttgatgg
     8221 agttcaacat attaacaatg ggcggtaaat caggagctat ggttggttat agtgaaattg
     8281 actcatcaca tttcacagac cgtgacaaac gcgttattag acgtgatcat gttaaagaag
     8341 cacaaagctt agtagagaac tataaagata cacaaagtgc tgatgctagg atgaaagcca
     8401 aacaaaaagt taacacatta agcaaaccgc atcaaaacta tttcaataaa caaattgata
     8461 aggtttataa tggattacaa cgctaatcca aagtaaatta taagttatac atctcgtttt
     8521 taaatgacaa tttatccccg taaatattat aaataatctt ttcaaattcc acatagatat
     8581 agagacacta ataaacctct ttgtctcgat atgatagtct gcaacgattc atgttgtagg
     8641 ctttttaatt ttacaaataa ggctaaatat ataagttctg gcacctaaaa tatagaaaat
     8701 acataaaagt aagtatagtt
//
LOCUS       DALAGF010000111         8566 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.111, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000111 DALAGF010000000
VERSION     DALAGF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8566
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.111"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            159..1688
                     /gene="aur"
                     /locus_tag="O2692_002319"
     CDS             159..1688
                     /gene="aur"
                     /locus_tag="O2692_002319"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HCZ4299928.1"
                     /translation="MRKFSRYAFTSMAALTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            2055..2504
                     /locus_tag="O2692_002320"
     CDS             2055..2504
                     /locus_tag="O2692_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCZ4299929.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            2507..2674
                     /locus_tag="O2692_002321"
     CDS             2507..2674
                     /locus_tag="O2692_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299930.1"
                     /translation="MYQMNNKNLFRITCTQVSYQILTCVRVFLWKERIHSHSIDCIKN
                     FIQHVFMGVFE"
     gene            2851..4086
                     /gene="arcA"
                     /locus_tag="O2692_002322"
     CDS             2851..4086
                     /gene="arcA"
                     /locus_tag="O2692_002322"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCZ4299931.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            4119..5129
                     /gene="argF"
                     /locus_tag="O2692_002323"
     CDS             4119..5129
                     /gene="argF"
                     /locus_tag="O2692_002323"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCZ4299932.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            5220..6650
                     /gene="arcD"
                     /locus_tag="O2692_002324"
     CDS             5220..6650
                     /gene="arcD"
                     /locus_tag="O2692_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCZ4299933.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSVIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFSMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYTIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            6667..7608
                     /gene="arcC"
                     /locus_tag="O2692_002325"
     CDS             6667..7608
                     /gene="arcC"
                     /locus_tag="O2692_002325"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCZ4299934.1"
                     /translation="MKEKIVIALGGNAIQTTEATAEAQQTAIRCAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFVEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            7707..8411
                     /locus_tag="O2692_002326"
     CDS             7707..8411
                     /locus_tag="O2692_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCZ4299935.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLICHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
BASE COUNT         3149 a         1180 c         1650 g         2587 t
ORIGIN      
        1 ttagctacag ataacatttt taaaaaagaa aagaatcaat aaataaaatc aacgaacaaa
       61 aagtatagaa ataaatagaa ataatcattt acttttctga aaaattaaat taatatttta
      121 tttataagct gtttttaaga tttcaggagg aatgaaatgt gaggaaattt tcaagatatg
      181 catttacaag tatggcagca ttaaccttgt tgagcacttt atcaccagca gcattagcga
      241 ttgattcaaa aaataaacca gctaattctg atattaaatt tgaggtgact caaaagagtg
      301 atgcggtcaa agcattaaaa gaattgccta aatccgaaaa tgtaaaaaat atttatcaag
      361 attacgctgt tactgatgta aaaactgata aaaaaggatt tacgcattat acattgcaac
      421 cgagtgttga tggtgttcat gcacctgaca aagaagtgaa agtacacgca gacaaatcag
      481 gaaaagtcgt tttaatcaat ggggatactg atgcgaagaa agtaaagcca acgaataaag
      541 tgacattaag taaagatgac gcagccgaca aagcatttaa agcagttaag attgataaga
      601 ataaagcgaa aaatcttaaa gataaagtca ttaaagaaaa caaagttgaa atcgatggtg
      661 acagtaataa atacgtttat aatgttgagt taattacagt gacaccagaa atttcacatt
      721 ggaaagttaa aattgatgct caaactggcg aaattttaga aaaaatgaac ttagttaaag
      781 aagctgcaga aactggtaaa ggaaaaggtg tacttggcga tacaaaagat atcaatatca
      841 atagtattga cggtggattt agcctagaag atttaacgca tcaaggtaaa ttatcagcat
      901 ttagctttaa tgatcaaaca ggtcaagcaa cattgattac taatgaagat gaaaacttcg
      961 taaaagatga gcaacgtgct ggcgtagatg caaattatta cgctaaacaa acatatgatt
     1021 attacaaaga cacatttggt cgtgaatcat atgacaacca aggtagtcca attgtttcat
     1081 taacgcatgt taataactac ggtggtcaag ataacagaaa taatgccgca tggatcggtg
     1141 acaaaatgat ctatggtgat ggtgatggtc gcacattcac aagtttatcg ggtgcaaatg
     1201 acgtagtagc acacgaatta acacacggtg tgacacaaga gacagcgaac ttagaatata
     1261 aggaccagtc aggcgctcta aatgaaagct tttcagatgt ttttggatac tttgtagatg
     1321 acgaggattt cttaatgggt gaagatgtct acacacctgg aaaagaggga gacgctttac
     1381 gcagcatgtc aaacccagaa caatttggtc aaccagctca tatgaaagac tatgtattca
     1441 ctgaaaaaga taatggtggc gtacatacga attctggaat tccaaataaa gcagcttata
     1501 acgtgattca agcaataggg aaatctaaat cagaacaaat ttactaccga gcattaacgg
     1561 aatacttaac aagtaattca aacttcaaag attgtaaaga tgcattatac caagcggcta
     1621 aagatttata tgacgagcaa acagctgaac aggtgtatga agcatggaat gaagtaggcg
     1681 tggagtaaaa atatataaac aagaagaagt aatgttaaac acttataaat aattaaattt
     1741 taaatacagc ttatatatgg ggtataccta cattgataat tgactttgta ggtataccct
     1801 ctctttgtaa ttagatgaga ggagtgtgag ggttgtctgc cgaaagacta ctcggcagtc
     1861 taaaatcatt acaagtagta gatatgtgat aattaaatgc tgacttagaa tacaaaattc
     1921 attttaaaag ttgtcacaaa aaatttacat gtatttttat tatattttgc aaaacaaagt
     1981 gttaaattat aaatgaaaca tgcatgaatt tattttttaa tacaagaaac gtaactacca
     2041 aaggagttta caatatgaag aaaagtaaac gattagaaat tgtttctaca atagttaaaa
     2101 agcataagat ttataaaaaa gaacaaatca tttcatatat tgaagaatat tttggtgtaa
     2161 gatatagtgc aacaacaatt gctaaagact tgaaggaact aaatatatat cgtgtcccta
     2221 tcgattgtga gacatggatt tataaagcta ttaataatca aacagaacaa gagatgagag
     2281 aaaagtttag acactattgt gaacatgaag ttctaagttc aatcatcaat ggttcataca
     2341 ttatcgtcaa aacctcacct ggtttcgccc aaggcataaa ctattttatc gatcagctaa
     2401 atatagaaga gatattaggt acggtgagtg gaaatgacac tacattaatc ttaactgcct
     2461 caaatgatat ggcagaatac gtatatgcaa aattatttaa atagacatgt atcaaatgaa
     2521 taataaaaat ttgtttcgta tcacgtgtac tcaagttagt taccaaatat taacttgtgt
     2581 acgcgttttt ttatggaaag aaagaattca tagtcattca attgactgta taaaaaactt
     2641 tatacaacat gtttttatgg gtgtttttga ataaaaaatg tatattttga cccaaaatgc
     2701 ctttatttat gtataaaaat ccattattat gtattgtata acaaaaagat atgaaatttt
     2761 cgactttctt tatgtgaata taatcacatg taagcgtttg aagattgtct atactctaaa
     2821 tgaattcaaa gataaaagga ggaaatagac atgacagatg gtccaattaa agtaaatagc
     2881 gaaattggag ctttaaaaac tgtgttactt aagcgtcctg gaaaagaatt agaaaattta
     2941 gtacctgatt atttagatgg attactattt gatgatattc catatttaga agtagctcaa
     3001 aaagagcatg accattttgc gcaggtgcta agagaagagg gtgttgaagt actttacctt
     3061 gagaagttag cagctgaaag tattgaaaat cctcaagtaa gaagtgaatt tattgatgat
     3121 gtattagcag agtctaaaaa aacaatatta ggtcatgaag aagaaattaa ggcattattt
     3181 gcgacacttt ctaatcaaga acttgtagat aaaataatgt caggggtacg taaggaagaa
     3241 attaatccga aatgtacaca tctagtagag tatatggatg ataagtatcc attctattta
     3301 gatccaatgc caaaccttta ttttactaga gatccacaag cctcaatagg acacggtata
     3361 acaatcaatc ggatgttctg gagagcacga cgacgagaat caatatttat tcaatatatt
     3421 gtaaagcatc atcctagatt taaagatgcg aatattccaa tctggttaga tcgagattgc
     3481 ccattcaata ttgaaggcgg cgatgaactt gttttatcta aagatgtctt ggctataggc
     3541 gtttcagaac gtacatctgc acaagctatt gaaaagttag cgcgacgtat ttttgaaaat
     3601 ccgcaggcga cgtttaaaaa agtagtagca attgaaattc caactagtcg aacttttatg
     3661 cacttagata cagtatttac aatgatagat tatgacaaat ttacaatgca ttcagccatt
     3721 ttaaaggcag aaggcaatat gaatatattt attattgaat atgatgacgt aaataaagat
     3781 attgccatca aacaatctag tcatttaaaa gatactttag aagacgtact aggtatagat
     3841 gatatccaat tcattccaac aggaaatggt gatgtcattg atggtgctag agagcaatgg
     3901 aatgatggct caaatacatt atgtataaga ccaggcgttg tagtgactta cgatagaaac
     3961 tatgtatcga atgatttatt gagacaaaaa ggcattaaag tcattgaaat atctggtagc
     4021 gagttggtac gtggacgtgg gggccctaga tgtatgagtc aaccattatt cagagaagac
     4081 atttaacgta ataaattata gaaacgaggg tgaaaataat gacagaaatt caaaaaccgt
     4141 atgatttaaa aggcagatca ttattaaaag aaagtgattt taccaaagca gaattcgaag
     4201 gacttattga ttttgcaatt acattaaaag agtataagaa aaacggtatt aagcatcact
     4261 acttatctgg aaaaaatatt gcactactat tcgaaaagaa ttcgacgaga acgcgtgctg
     4321 cgtttacagt tgcgtctatt gatttaggtg cgcatccaga atttttagga aaaaatgata
     4381 ttcaattagg caaaaaagaa tctgtagagg atactgcgaa agtattaggt agaatgttcg
     4441 atggtattga attccgtggt ttttcacaac aagctgttga agatttagcg aagttctctg
     4501 gtgtaccggt gtggaatgga ttaacagacg attggcatcc tacacaaatg ttagctgatt
     4561 ttatgacaat aaaagagaat tttggatatc tagaaggaat aaacttaact tacgttggag
     4621 atggacgtaa taatattgcg cattcattaa tggtagcagg tgctatgtta ggtgttaatg
     4681 taagaatttg tacacctaaa tcattaaatc caaaagaggc atatgttgat attgcaaaag
     4741 aaaaagcgag tcaatatggt ggttcagtca tgattacgga taatattgca gaagcagttg
     4801 aaaatacaga tgctatatat acagatgttt gggtatcgat gggtgaagaa agtgaatttg
     4861 aacaacgtat taatttatta aaagactatc aagtgaatca acagatgttt gatttaacag
     4921 gtaaagattc aacgatattc ttacattgtt taccagcatt ccatgataca aatacacttt
     4981 atggacaaga aatttatgaa aaatatggat tagctgaaat ggaagttaca gaccaaatct
     5041 ttagaagtga acattcaaaa gtgtttgatc aagctgaaaa tagaatgcat acaattaagg
     5101 cagtaatggc agcaacattg gggagttaat cactaaatgg aacgatatga atatgatgtg
     5161 tctgatgata taagtgtcat gtacagacac ctcatattgg tattaaagga gaaatgaata
     5221 tgaacgaatc aggagataac aaactcagta aatcttcttt aattggacta gttataggat
     5281 ccatgattgg tggcggtgcg ttcaatataa tgtctgatat gggcggtaaa gccggtggat
     5341 tagccattat tattggttgg attattacag ctataggaat gatttcatta gcgttcgtat
     5401 ttcaaaattt aaccaatgaa cggccggagc tagacggtgg tatttatagt tatgctcaag
     5461 caggatttgg cgattttgta ggatttatca gtgcttgggg atattggttc tcagcgtttt
     5521 taggcaatgt tgcctatgca acactattga tgtcagcagt aggtaacttt ttcccgattt
     5581 ttaaaggagg caacacatta ccaagtgtta ttgtcgcctc gttactactc tggggtgtcc
     5641 atttcttgat tttaaaaggc gttgaaacag cagcatttat caatagtatt gttactgttg
     5701 caaagttaat accgatttta cttgtaatca tatgcatgat aattgcattc aattttgaca
     5761 cttttaaaac aggctttttc agtatgacgt cagagggtgt attgccattt agttgggcga
     5821 gcacaatgag ccaagttaaa agtacgatgc tagtgacagt ttgggtgttt atcggtatcg
     5881 aaggtgcagt aattttttct agtagagcta aaaataaaaa agatgtaggt agtgccacgg
     5941 ttataggact tatatcagtt ttaattatct atttcttatt aactgtatta gctcaaggcg
     6001 tgattttgca aaatcatatt tcgcaattag attcgccaag tatggcacag gtgcttgcaa
     6061 ctattgtagg tggttgggga tctacacttg taaatattgg tttaattatt tcggtactag
     6121 gtgcatggtt aggatggaca ctgcttgctg gtgaattacc tttcattgtt gcaaaagatg
     6181 gattatttcc aaaatggttt gctaaagaaa ataaaaatgg agcacctgta aatgcactgc
     6241 ttattaccaa tatattagta caattatttt taataagtat gctatttaca cagagtgcgt
     6301 atcaatttgc attttcacta gcatcaagtg ctattttata cccttacatg ttcagtgcat
     6361 tttaccaagt taaatacact ttagagcatc gacagcaagc aactactaaa caatggacga
     6421 ttggtatcat agcctcaatt tatgctatat ggcttatata tgcagcaggt atcaattact
     6481 tattattgac gatgttactt tatattccag ctcttcttgt ttatacaatc gttcaaaaga
     6541 ataatcagac acgtttgatt aaatcagact atattctttt tatgattatt atcgtacttg
     6601 cagttatcgg gttaattaag ttattgatgg gaacgataaa tgttttttaa aaggagcgac
     6661 aaaaatatga aagagaaaat tgtcattgca ttaggcggta atgcgataca gacaacagaa
     6721 gcaacagctg aagcacaaca aacagctatt agatgtgcga tgcaaaacct taaaccttta
     6781 tttgattcac cagcgcgtat tgtcatttca catggtaatg gtccacaaat tggaagttta
     6841 ttaatccaac aagctaaatc gaacagtgac acaacgccgg caatgccatt ggatacttgt
     6901 ggtgcaatgt cacagggtat gataggctat tggttggaaa ctgaaatcaa tcgcatttta
     6961 actgaaatga atagtgatag aactgtaggc acaatcgtta cacgtgtgga agtagataaa
     7021 gatgatccac gatttgataa cccaactaaa ccaattggtc ctttttatac gaaagaagaa
     7081 gttgaagaat tacaaaaaga acagccagac tcagtcttta aagaagatgc aggacgtggt
     7141 tatagaaaag tagttgcgtc accactacct caatctatac tagaacacca gttaattcga
     7201 actttagcag acggtaaaaa tattgtcatt gcatgcggtg gtggcggtat tccagttata
     7261 aaaaaagaaa atacctatga aggtgttgaa gcggttatag ataaagattt tgctagtgag
     7321 aaattagcaa cgctgattga agcagatacc ttaatgattc ttacgaatgt agaaaatgta
     7381 tttattaact ttaatgaacc taatcaacaa caaatcgatg atattgatgt agcaacactg
     7441 aaaaaatacg cggcacaagg taagtttgtg gaaggatcga tgttgccaaa aatagaagct
     7501 gcgatacgat ttgttgaaag tggggaaaac aaaaaagtta tcattaccaa tttagagcag
     7561 gcatacgaag ctttgattgg taataaaggt acacacattc acatgtagca ctttatcacg
     7621 cgacaaaaca ttaaatatgt ttctccgttg attcaaatga aaaagttgtc tgctgacact
     7681 ttgcaaggtt tgaaggagtt taacttatga cagaaaactt tattttgggt agaaataata
     7741 aattagaaca tgaactaaag gcattagcag attacattaa tataccatat agtatattac
     7801 aaccatatca aagtgaatgt tttgtcagac attatacgaa aggccaagtt atttattttt
     7861 cgccacaaga aagtagcaat atttactttt taattgaagg taacattatt agagaacatt
     7921 acaatcaaaa tggagatgta tatcgttatt ttaataaaga gcaagtatta tttccaatca
     7981 gtaacttatt tcatccgaaa gaggttaacg aattgtgtac agcattaacc gattgtacag
     8041 ttcttggatt gcctagagaa ttgatggcct ttttgtgcaa agctaatgat gatatatttt
     8101 tgacactttt tgcattaata aatgataatg agcagcaaca catgaactat aacatggcat
     8161 taacaagtaa atttgctaaa gatcgaatta tcaaattgat atgccatcta tgtcagacag
     8221 taggatacga tcaagatgaa ttttatgaaa tcaaacagtt tttaactatt caactcatga
     8281 gtgatatggc tgggatttcc cgggaaacag ctggtcatat tattcatgaa cttaaagatg
     8341 aaaaacttgt tgttaaagat cataaaaatt ggttagtaag caaacattta ttcaatgatg
     8401 tatgtgttta atatacaatg taaaatgaat aagttgaaca tgaggtctaa cgtacattta
     8461 tacgttaggc cttttttgct agcatgatga ataatttaaa atgttagtta aatttgattg
     8521 ttgaaattac agtaaaattt aaggtgatga aaaatttaga acttct
//
LOCUS       DALAGF010000112         8544 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.112, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000112 DALAGF010000000
VERSION     DALAGF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.112"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(123..272)
                     /gene="rpmG"
                     /locus_tag="O2692_002327"
     CDS             complement(123..272)
                     /gene="rpmG"
                     /locus_tag="O2692_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCZ4299936.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            complement(363..1880)
                     /locus_tag="O2692_002328"
     CDS             complement(363..1880)
                     /locus_tag="O2692_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HCZ4299937.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            2084..3538
                     /locus_tag="O2692_002329"
     CDS             2084..3538
                     /locus_tag="O2692_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ4299938.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
     gene            3677..3781
                     /locus_tag="O2692_002330"
     CDS             3677..3781
                     /locus_tag="O2692_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299939.1"
                     /translation="MLPFFILDILHIVYNIDNNNFSGVKFNDWTNKFI"
     gene            3756..4070
                     /locus_tag="O2692_002331"
     CDS             3756..4070
                     /locus_tag="O2692_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299940.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFMHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(4363..5166)
                     /locus_tag="O2692_002332"
     CDS             complement(4363..5166)
                     /locus_tag="O2692_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCZ4299941.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL"
     gene            complement(5224..6138)
                     /locus_tag="O2692_002333"
     CDS             complement(5224..6138)
                     /locus_tag="O2692_002333"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCZ4299942.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            complement(6140..7201)
                     /locus_tag="O2692_002334"
     CDS             complement(6140..7201)
                     /locus_tag="O2692_002334"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCZ4299943.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            complement(7207..8487)
                     /locus_tag="O2692_002335"
     CDS             complement(7207..8487)
                     /locus_tag="O2692_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HCZ4299944.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEL
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
BASE COUNT         2625 a         1609 c         1233 g         3077 t
ORIGIN      
        1 gttctaccga tgaaactcaa tccattaatc actcttcaca aaaatttcat aaaaaagcga
       61 gctccccaat ataataggta agcccgcttt attttcaaat cgtcgtattt gaatgataag
      121 aattacttag tttcacgatg taacgtatat ttgtttaatc ttgggcaata ttttttcatt
      181 tcaatacgct caggattatt acgtttattt ttagtagtga tatagttacg atcgccacat
      241 tctgtgcatg ctaatgttac gtttacgcgc acgttaaaca ccctctcttg ttaggaatct
      301 ttacgattta ttatgatatc ataaactgct caactacgca atatagaaac tacatatcaa
      361 atttattttt caaagttttc gtatgtttca tcattttcag tttcaagatc aatagaattt
      421 atatcaatac ctaatgcttt tgcaacacct ttaccatatt ctgggtcagc tttgtaacaa
      481 tgacgaatat gacgtcgttt aacatcatcc gttacgcctt ccattgcatt tgctgtattt
      541 gtaaaaattc tttctttagc gtcctctgat tgtaatctaa acaatttacc tggttgttca
      601 aaataattat catcatcttg acgttgatta tattcatagc cgtatccatc agttgggaat
      661 ggtggctttt tatattcagg ttgagaatca aatttaccat ggttatttgg ataataatgt
      721 gttcctccac cttggttatt gtcaactacg cgcatttgac catctctact aaaaggacaa
      781 atattttcaa taccaacacc tttaggttgg tttacaggaa tctgccaatg attaactcct
      841 aatcgatatc tttgcgcatc gccatatgag aataaacgcc cttgcagcat tttgtctgga
      901 gaaaaatcta atcctggaat aatattagtt ggtgcaaacg cagcttgttc aacatccata
      961 aagtaattat ctggatttct atttaattca aactctccaa cttcaattag aggatactca
     1021 tcgtgatacc atacttttgt taaatcaaat ggattatctt tatggttttt agcttgttcc
     1081 tcagtcatta cttgaatata cattgtccat tttggataat cacctttttc aatggcttcg
     1141 aataaatcgc gttgagatga atcacgatct gtagctataa tttcagcagc ttcttcatca
     1201 gttaagtttt caataccttg ttgcgttcta aaatggaatt taacccaaac acgttcacca
     1261 gaatcattat acatagagta tgtgtgagaa ccgaacccat gcatatgacg taaatcttta
     1321 ggaatccctc tatctgacat taagatcgtt acttggtgca atgcttctgg aagacccgtc
     1381 cagaaatccc agttattttg tgcatctctc atatttgttc taggatctcg tttcaccgca
     1441 cgatttaaac taacaaataa ctttggatct ctaaagaaga atactggtgt gttattccct
     1501 actaaatccc agttcccttc ttcagtgtag aactttaacg caaatcctcg aatgtcacgc
     1561 tccgcatcag cagcaccacg ttctcctgct acagtagaga aacgggcaaa catttcggtt
     1621 tgcttaccta tttcagagaa tattttagca ttcgtatatt ttgttatatc tttagttaca
     1681 gtaaatgtcc caaatgcacc agaacctttg gcatgcattc gacgttctgg tattacttct
     1741 ctatcaaatt gagacatttg ctctaaaaag taaatatctt gcattaaaag aggtccccta
     1801 ggccctgctg tcatactatt ttctcggtct gatactggat gcccaaaaac accagttaac
     1861 tttttgtctt gttgtgacat agtcatccct ccacaattta taataattat aatttaatac
     1921 atattataat ttaaccccat caaaattaca attaaacatg ctcaagatga ttaaaaaatc
     1981 agaaaatttt aattttttcg ctttacagta ctaaatagtt attgaatagt acacttaaaa
     2041 agcttacaat aatgggtata tttaaataga tgggagacta aatatggaag ataataaaat
     2101 gaaccgtagt cttaactcaa gacacatttc catgattgct ataggtggtg caattgggac
     2161 tggtctattt gtagctactg gtaatatcat ttctcaagct ggtcctggag gcgctatact
     2221 cgcttatctt gttattggtg tcatgctata tttcttaatg tcatcaattg gagagttggc
     2281 aacattttat ccagtatcag gttcattcag ctcttattca acacgcttta ttgactcatc
     2341 tcttggcttt accatgggat ggttgtattg ggcattgtgg tcattagtta caagtgttga
     2401 tgtcatagta gcgtcaaatg tgctttattt ctgggacaca tttaaatttt tccaccccat
     2461 tacttggagc ttaatcttta ttacaatttt actattatta aacatttttt ctgtaaaatc
     2521 atttggagaa actgagtttt ggttatcatt gattaaagtg ttaacaatta tcgtattcgt
     2581 tatttttggc tttttaatga ttttcggtat cttaggtggt catacatatg gatttgaaaa
     2641 ctatacaaaa ggccaagcac cgtttgttgg tggtatctct ggtttcttag gcgtattatt
     2701 agtcgccgga ttttcggttg gtggtacaga agtagtagca gtaactgctg gtgaatcaga
     2761 tgaccctaaa aagtctatgc ctaaggcaat taaacaagta ttttggcgta ttcttttatt
     2821 ctatgtctta tcaattgcag taattggtgc aattattccg tacacagatc catcattatt
     2881 aagagcaagt agttcaataa gtcaaagccc atttacaatt gtattcgata gagtaggcat
     2941 agcctttgca gcatcagtaa tcaacgcggt tattttaact tcattattat ccgctgcaaa
     3001 ttcaggtgtt tatacaacag gcagaatgtt gtattcctta agttcagaca aaaaagcacc
     3061 ccaattttta agtaaattaa acaagacaac taagttacct ttaagagcat tattaactac
     3121 ttatgcagtc gttgttattg ttattattta tgcaaacttt aattcaaatg ccgtttttaa
     3181 tttacttgaa attattggtt caatgattat agttgtttgg ggatcaagca tttggtcaca
     3241 aatacgattg cgacaagcta ttaaaaaaca aggtcaagac cctaataagg tcctaccata
     3301 taaagcacct ttttatccat taggaccaat cattgtcatc actacactat tattcttgct
     3361 atttggtggc tcagttgaat atattttaaa agatcaatgg ttaaatgctt ttaaaaactt
     3421 tttaccttta atcattctag cgttgattta ctttattcat aaaatcattc acaaaacaaa
     3481 atttgtaaag ctagaaacaa ttaatttaaa accacacgat tatgacaatc aaaaataaat
     3541 aaattctcat acggcagatg ctttagatta tctatagcat ttgccgcttt ttaatggtgt
     3601 aaagcagata tactttaata tctttttcta gtataaagtg tattaactag tattctgttt
     3661 atattttatt attaccatgt taccattctt tattttagat attttgcata tcgtgtataa
     3721 tatagataac aataactttt caggggtgaa attcaatgat tggacaaaca aatttatttg
     3781 atgatgtact aaagcaagat gaaagatttg ttattgtagt tcaagcatta gaagaaaaga
     3841 acggacaatt attaaagaga actttaaggg aatatcccgg tttaaaccat aaacaaatga
     3901 atgatttatt tatgcactta aaggaattat tttccgaaga atcatttgct gaaaaccaat
     3961 cagcgttcag tattacagtt tatacaaatt tagattatac tgctgaccaa atatatgctc
     4021 atgtaaaacg tttcagaggt aagcatgact ggacacaaac agctaaataa aatcaagatg
     4081 cttgcagtga ttttaacaat taatgttaga ttgtcactgc attttttaat gcattcgtta
     4141 ttattcagaa tgatcaaatt tatttatcta ccttccgtga ctagttaata cttgatttat
     4201 tcaaaaaata tacatttact ctattcgttt gatatcatta ctaatctaaa atatgtattt
     4261 caaatcctcc atagtataac tgaagtcatt ttcacaaaaa taaaaagcct taaccacaag
     4321 atatcttatt caaatatcat gtgttaaggc ttattttaaa atttataaca attttttgag
     4381 cgcatgtgga ataccatttt cattatgatc caacgtaact tcatttgcat attgcttaag
     4441 ttcatctgat gcattcccca tagcaatagc tagacctgcg acttcaaaca ttgatttatc
     4501 attcatacta tcaccaaaag caatgacttg atccaccgaa tatcccaatt tgtgacataa
     4561 cgctttaatt gcattacctt ttgaaacgtc tttggccata aattctaaga agaatggctt
     4621 acttgtcgta gcatctacat tatcattgaa cacaccattc aaatcaattc tagcttctgt
     4681 aatattcgct acataatcta cacccatgac cttgggtaca tcgccttgaa tatacgcttt
     4741 gatatcatca acacgtttca tcggtaaacc tgttaattct gcttcaatat tcatatactc
     4801 atgttcgctg tcgtaaataa tttgaccatc atgatatgta agaacgaaaa agcctctatc
     4861 tcgacaataa tctacaattt catcgaaatc ttgcttacca atcgatttac ttacttctac
     4921 ttcttcatta gtcatgttaa tcgttttact accgttataa ctaataatat agctatgatg
     4981 ttcaggtaat tttaaatctc tagcagttgg aatcatacct tcagtaggtc taccagatgc
     5041 tagaacaaca taataacctt catcttgaat tgctgttaaa taatttgcag tttcttctga
     5101 tatcacatta tctgaattta gcaatgtgtc atccatatct aaaacgacaa ctttataatt
     5161 tgtcatatcg cattaaatct cctctctatt ctgcatttaa tactatttta caatataaag
     5221 cgtctattgg tatacaattc gttctttaac accactgata ttaatatcga ccaattcaga
     5281 atggcatgat actacctgac tatttaaaga gcgaaccaat tccccactat tttctttacg
     5341 actaaatatt aaaatagttg gtccagcacc actaattaca gttgcatagg cattatgttg
     5401 actagcaatt gttttcactt catcaaattc agcaattaaa tgctgacgat acggttcatg
     5461 aaagccatct tgttgcatga gtttacctgc taattcataa ttgtgctgtg ctaatgcaca
     5521 aatcattgta ttactaattg cactactttt aaccgcttca ctatgtgtta atttttgtgg
     5581 taaagcacgt cttgatgctt ctgtttttag ttcataagtt ggtatcgtta caatcacatc
     5641 cacgtctggt atgtcgatat gtgcaactga cgtttctttc gagacatcat tataatatcc
     5701 agcgattaaa ccaccataaa tggtcggcgc aacattatca ggatgtcctt cgatttcagt
     5761 cgctaattgt aatacctcat gtttagacag ttggatatca ccaaaataat ttgcgatata
     5821 tatagctcct actaaagcag aagctgacga acctaaccct cttgccaatg gaatatcact
     5881 tctcatttcg atacataaat taggcaagtc aacactatat ttagaagcaa cttgttgtgc
     5941 tacatgataa ataaagtttg tttcgtcagt aggcaattgc ttagatgcat catcatggaa
     6001 aatatattcc cattttgtcc ctgatgtttc ctttacagac agatgcaaaa atttatctaa
     6061 agccatacct atagaatcaa agccaactcc aaggttggct gttgatgcag gaattgttaa
     6121 ctccaaaaca ttcgacatgt tataaagctc ctttaatata atcgataatg ctatctttat
     6181 catttggcaa tggctttatc ggattatcta gtagtgaaat agcagtatca ggatctttta
     6241 atccattacc agttaaaata gcaactactt ttttaccttg aggtaattta ccttgtctat
     6301 gcaatttaat taaacctgca atagaagcat tactcgctgg ttcactaaag acaccttctt
     6361 tagttgtcat taactgatat gcttctagaa tttcatcatc agtaacacta tctattaatc
     6421 catttgattc tttaagagca ttagtcgcct tatcccaact agcaggatta ccaattcgaa
     6481 tagcagttgc aatcgtttca ggatttttaa tgactttatt ttgaacaatt ggtgatgcgc
     6541 cttcagcttg gaagccaaac attttcggca attgtgatcc tttagcttca tgatattctt
     6601 taaagccttt ccaatatgca gtaatattac ctgcattacc tacaggaatc gctaaaatat
     6661 caggtgcttc accgtctaat tgttgtacaa tttcaaatga gcctgtcttt tgtccttcga
     6721 ttctaaatgg attgacagag tttacaagct cgatttcgcc actttttgca atttctttta
     6781 caatttctaa agcttcatca aagtttcctt caatagaaac gatttctgca ccatacatta
     6841 ctgcttgcga caatttacct aatgcaattt taccttctgg tattacgacg atagctttta
     6901 aacctgctct cgctgcatat gctgctgcag acgctgatgt atttccagtc gaagcgcata
     6961 ttacaatttt cttaccttgc tcttttgctt ttgtcacagc cattaccata ccgcgatctt
     7021 taaatgaacc tgtcggattg gcaccttcat acttcacata taaatctatg cctagtattt
     7081 tagacatatt ttcacaatga atgagtggtg tatttccctc gttcaatgtt aattttggtg
     7141 tattttcatt tactggtaaa tgtgctttaa actcttctac taatccttgc catcttctca
     7201 ttataattaa actccttcta ctgggtatac tttgtcaact tcgtatccat gcttagtgat
     7261 taattcttcc ggtgatgatt ccaatccaac tataacagcg gcatatgatt gattatcgta
     7321 atttgcaact cttaatgatc ggtgaaatgg taatattgcc tttaactcat tttcaaaatt
     7381 ttcaattgaa cctttgacat gattcactac tacaaagaaa ttggattttt ctttaatatt
     7441 aatttctgca tctgaatcca tcatttcccg tgttttatct gtctttaatt caaaatgtgg
     7501 tggcaatgtg tgtaaatctg attcaaagaa taatgctaca ttcaataaat cactgacaac
     7561 ggcactacct gttgctaaac tgcctgctcc ttttccataa aacatcgtgt caccaacggc
     7621 atcaccaata acataaatcg cgttatattc atcctctaca gctgctaatt gatgcttttt
     7681 atcaattaac gttggttcta ccgatgcatt aactttgcca ttttcatata ttcccttacc
     7741 aatcaattta attttatacc ccagttgatc ggctacatta atatcagtta aagttacgcc
     7801 actaattcct cttcgtttaa cgtcgtttaa tttaatgact tggttaaatg ataaatatga
     7861 tgtaatgaca actttacgcg ctgcatctac cccttctaca tcatcagttg gatccgcttc
     7921 agcaaaacca agtcttttcg cttcatcaag tgcttcctca aatgtcgttt gctctttagt
     7981 cattttagat aaaataaaat tagaggtacc atttaaaatt cccataaatt ttgaaatatt
     8041 attcgcattc aaaccattat ttatggcatt tacgatcgga ataccacctg ctacactcgc
     8101 ttcaaacttt aaagctacac cattttcttc tgctaaatct tctaaaagtt taagatgtac
     8161 tgctaataaa tctttatttg cggtaataac atgtttttta tttttaagtg ctgttcttaa
     8221 ccaatctaca gttggttcaa ttcctcccat gacttcaacg ataatatcta atgaatcatc
     8281 atttaaaatt tcattaacat cttcagttaa atgatattgg ctaatattta gcggtctctt
     8341 tttagattta tctcgaacaa gaatatgctt tatgacaata tctttattta atgtatcttg
     8401 aatttgctgt cggttctctt cgatgatttt aacaacacca gatccgacag tacctaatcc
     8461 taataatgct atatttaatt ttttcatttt atgtacctcc gtttgtgctt ttgagaaaaa
     8521 caaatgtatt attgaaaaac acta
//
LOCUS       DALAGF010000113         8513 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.113, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000113 DALAGF010000000
VERSION     DALAGF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.113"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(1..346)
                     /locus_tag="O2692_002336"
                     /pseudo
     CDS             complement(1..346)
                     /locus_tag="O2692_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(408..1319)
                     /locus_tag="O2692_002337"
     CDS             complement(408..1319)
                     /locus_tag="O2692_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCZ4299945.1"
                     /translation="MKRLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKV
                     PEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGGDYIGENDVERVAK
                     AKPDLIVVDAMDKNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEW
                     DDKTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKD
                     KLQEDKKGYASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYK
                     AEDYWFTDPITLEHLRSKLKKEILNKK"
     gene            1480..2787
                     /locus_tag="O2692_002338"
     CDS             1480..2787
                     /locus_tag="O2692_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCZ4299946.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNR
                     FLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTS
                     VSAFNNAGFALFKNNLIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLH
                     SKLVLTTTSILIIIGAITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIA
                     SINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISD
                     KHIKLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYH
                     GITKIIIIFVMLCGKVGLLTLLRTFIPPKSPKNYRYTKGQIYL"
     gene            complement(3640..4083)
                     /locus_tag="O2692_002339"
     CDS             complement(3640..4083)
                     /locus_tag="O2692_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4299947.1"
                     /translation="MSIITRLFNNSDFEKLNQLCKLYDDLGYPTNENDLKKRLKKITN
                     HDDYFLLLLIKENKIIGLSGMCKMMFYEKNAEYMRILAFVIHSEFRKKGYGKRLLADS
                     EEFSKRLNCKAITLNSGNRNERLSAHKLYSDNGYVSNTSGFTKQL"
     gene            complement(4248..4625)
                     /locus_tag="O2692_002340"
     CDS             complement(4248..4625)
                     /locus_tag="O2692_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299948.1"
                     /translation="MFISLAIWSINSMGNKQCISLNLLLSDDTFAVISVMTTLGTAIL
                     GGIAPSIYGIFTSPIDKKSSFRAISDWEQEQRNNVKDLMMLLPFTLLSILIVIFIFQF
                     AYSKLFNTIAIIIFIIILVVMFF"
     gene            complement(4943..5533)
                     /locus_tag="O2692_002341"
     CDS             complement(4943..5533)
                     /locus_tag="O2692_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCZ4299949.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYNANSAHVTACRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNVPIFINIGEWDGG
                     DEGLDKAVKDVSNDNPNHTVIVDDIPLEDYESIRFL"
     gene            complement(5530..5742)
                     /locus_tag="O2692_002342"
     CDS             complement(5530..5742)
                     /locus_tag="O2692_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HCZ4299950.1"
                     /translation="MTDDNVNDHIIKNHIEMIVDRLATDKEFYIFDSLIQGLSYQDIS
                     SALDCSEQSVILWYETILDKIVGVIK"
     gene            5803..6349
                     /locus_tag="O2692_002343"
                     /pseudo
     CDS             5803..6349
                     /locus_tag="O2692_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(6444..8060)
                     /gene="groL"
                     /locus_tag="O2692_002344"
     CDS             complement(6444..8060)
                     /gene="groL"
                     /locus_tag="O2692_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCZ4299951.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEEGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(8136..8420)
                     /gene="groES"
                     /locus_tag="O2692_002345"
     CDS             complement(8136..8420)
                     /gene="groES"
                     /locus_tag="O2692_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCZ4299952.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
BASE COUNT         2737 a         1577 c         1033 g         3166 t
ORIGIN      
        1 gtttttcgta tttctattga ttctggtaca tctaaatcta tccatgatat tttttcttta
       61 ttaaatcttt gaaaccttgt atctaagcca caacctatat tgaccacgat taaatcttta
      121 ttatccttga taagcctctt agtaacacaa tctattatca cagtacgtat actaattcca
      181 atttgagaca tgtcatcaca tgtaacattt ttgtacatat catctaaacc atcaaatatt
      241 tttttgatag tgcgtctttt attattggtt tttcgttttc gtactctttt gctcgagcta
      301 tcaaaggaat taacattgat tcgggaattc caaccagttg cttcatttaa tcactcctta
      361 aaaacattag taccaatgca taagaaaatt tttacaactt atttctatta ttttttattt
      421 aaaatttctt tttttaattt acttctcaaa tgttctaatg taataggatc tgtgaaccaa
      481 taatcttctg ctttatatga aattacatgt ccttttttta cagcttcaat attctgccat
      541 gtatgtgttt tttcaaaatc aaattttccg tacgaaggtt tacttaaaaa tatataatca
      601 ccagcatatt tactaatatt ttcttttgaa atggaagcat agcctttttt atcttcttgt
      661 aatttatctt tatattgctt tgtcattggc ataccgaatg catcatgaac aatatccaaa
      721 ccacgacccc atgtagagtt atatatgtat atttgctttt catctggttc aaacacagat
      781 gctgttgctt gaccaatttt actttgaatt tcttttttat cttttctagt tttatcgtcc
      841 cactcttcaa tccatttttt agctttatct tcattattag tcaacttgcc tatttctttt
      901 aatatttctt tatgattgta tttattgtat gtatatggga ttgttggcgc tattttttgg
      961 tattttttta tatttttatc catagcatct acaacaatta aatctggttt tgctttagca
     1021 actctttcaa catcattttc tccaatataa tcaccccctt ttaaataagg ttttaaaatt
     1081 gaagaatctt tagttatatc tgaaacagca attggtttga ttcctaactt tataaaatca
     1141 ccaacataaa atccagttaa tacagcaact cttttaggtt tctctggtac tttcagtgtt
     1201 tcaccatttt caattttata gtctgtagtc tttgtcgagt tacttgtttt atcgacagat
     1261 gctgaacacg cagtcaaaat aaataaatta cataataaaa ttccaattaa tcttttcata
     1321 atatcatcct tttgtttttc aattgatatt cattttcaat tataaaatat taaaagaatt
     1381 agtcaacgcc tgttagtaat acacatcagt aacatttcta ttttcattta tgatattatc
     1441 taattattaa tttataatat aaaggagtat gaaattaaaa tgaacaaagt ccacaaacct
     1501 ttatattttt atttaatgct tttcttctct acaactatca taggtgcact tttattgtat
     1561 ttacctttta ctggtaaaaa gccaatatct tttttagatg ccctatttat agcttcaagt
     1621 gcatttacag ttactggctt gtccccagtt gatataggat cacagtttaa tatacttggt
     1681 gagatagtaa tactattatt aattcaaata ggtggtctgg gtatcgtgac cgtaacccta
     1741 ttgacactag tatttttaaa tagaaagata tcaatgaaaa atagattctt gattatggtt
     1801 acatggaata ttgacgaacc tggtggtgtt attaagctaa ttaaacactt ggctatttat
     1861 agtttagtca ctgaattaat tggtatgatt tgtttgtgtt tatcttttat accaaaattt
     1921 ggtataggca aaggtttatt tttaagctta ttcacatcag tatcagcttt taataatgct
     1981 ggatttgccc tttttaagaa taacttaata gattattcta gtgatccaat tgtcattatt
     2041 acaatctcaa tactcataat atttggaggt attggacatt ttgtcgtgat agactttatt
     2101 aattgtaaaa aattgagtaa attatcttta cattctaaat tagtcttaac tacaactagt
     2161 atcctaataa ttataggagc tattacattc tttttattag aacagtttaa tactatgcaa
     2221 catatgggac tagttgaaaa aatcggaaat tcttttttcc aatcagtaac aacacgaaca
     2281 gcgggtttta acagtataga tatagcaagc attaacaaat ctaccgcatt aatgttaatg
     2341 ctacttatgt ttattggtgg tgcccctctc agtgcagctg gaggaattaa aataactact
     2401 tttgcagttg cgtttatttt tgtactaaat tatatacgta aagaaaataa tgtttcagta
     2461 ttcaataaag aaatatctga caaacatata aaactatcta ttgttaccat taatatctca
     2521 tttctattta tcagcatcat tacttttata ttatcgataa ttaatccgaa catatcatta
     2581 atcaagttat tattcgaagt ggtttctgca ttcggaacag tagggttaag tatgaacctt
     2641 accacagaat atcatggtat tactaaaata attattatat tcgttatgct ttgcggtaaa
     2701 gtaggactat taactttatt aagaacattt ataccaccaa aaagtcctaa aaattaccgc
     2761 tacactaaag gacaaattta tctataaaat aactatccat ctttaataag aaatgaacta
     2821 gtgttttaat ctattacatt aaaaaaacta cctaaacagg tagtttttta tttattttag
     2881 gccgattaaa tgtcttatgt ctttaattaa gtaaaatgca caagtacacc tttaaaaaga
     2941 tttactcaaa actaaactat acctagttaa ccgcttgtga ttaagaaatt ttaaatgtgg
     3001 tttcttttaa ttatgtcaac cacccataag gattttaatc aagcactcat tcattcctgc
     3061 actcgcaata tgcattactt atagaagtgg atgcactctc atattatggc aataccataa
     3121 ctcccgcacc ataatgattt aaataccatt atgaatctca attacaaatt agcacataaa
     3181 aaagacgcct cccataagtc tagtcaccaa atggaaaacg tctcgtaatt aaaatatatt
     3241 tgcgtgcatt ctaatttgta cttagaatac atcatactcg ttcataatgt aattttctaa
     3301 ttagtcaaaa ctatatacag ttttacatca ttcccggcat tatgcactaa taaaaactta
     3361 ctttaataca cgctttaaag attattaaat cagcgtttta atttttagac aacgctatta
     3421 tttttagttt ttcaattcta ttatgtcata attatgtcac tcaaaaactg ttttccaaca
     3481 ttgatttatt gttggaaaat ctcgaaaatc tagtctattt ctccaacaat cgaagaatta
     3541 tgcccttttt tctcccttta aataagtcat aatacgaggc atacatgcaa catttacaat
     3601 aaaatatgtt cctacatcgt attatacgaa tgctcacact tatagttgtt tagtaaaccc
     3661 agatgtattg ctaacatacc cattatcact atatagttta tgtgcagata gtctttcatt
     3721 tctattacca ctatttagtg ttattgcttt acaattcaac cgtttagaaa attcttcaga
     3781 atcagctaat aatctctttc cataaccttt tttcctaaat tcagaatgta taacaaacgc
     3841 aaggattctc atatactctg catttttttc gtaaaacatc attttacaca taccacttaa
     3901 accaattatt ttattttctt ttatcaaaag cagtaggaag taatcatcat gattcgttat
     3961 tttctttagt ctctttttta aatcattctc atttgttgga taacctagat catcatataa
     4021 tttacatagt tgatttaatt tttcaaaatc actgttatta aacaatcttg taattatgct
     4081 catataaccc accctaaaaa tattatgtac ctattgttac atacaataga tacgcaaaat
     4141 catttctcac tgcaaatcag gacgtttctc agcgtaaaca aatcatttct attcttttga
     4201 aaaatatctg catatcttca cagtaatttt ataacagatt aataaaatta aaaaaacata
     4261 acaacaagta ttataataaa tattataata gctatcgtat taaatagttt agaatatgca
     4321 aactgaaaaa taaaaatcac aattaaaata ctaagtaatg taaatggtaa tagcatcatc
     4381 agatctttaa cattatttct ttgttcttgc tcccaatcgc taatagccct aaaactactt
     4441 tttttatcta taggcgatgt aaaaatacca tatattgaag gtgctatacc tcctaaaata
     4501 gcagttccca aagttgtcat tactgaaatt actgcgaaag tatcatccga aagcaataaa
     4561 ttcaaactaa tgcattgttt attacccatc gaatttattg accaaatagc tagagaaata
     4621 aacaacccaa aatttaaaat aaatgatata gtaatagcaa ttgtttacaa aacacgaaat
     4681 ttttcatttt tatttatatt atccattttt ctcccttttt cttaaatcat tttattatat
     4741 attacaataa tcaatctgaa atgttgatgt aatttgaaaa aaatatcata cttttactcc
     4801 tgaaaacctc cctaaatcat caatatgaaa atcagtatta ggatattgcg tttacaactc
     4861 ttttaaatct cactcattct tctcatcgtc ttaaccgata ctatcaataa atactggcaa
     4921 caccataaaa tatataccca atttataaaa acctaattga ctcataatct tctaacggta
     4981 tatcatccac aatcacagta tgattaggat tatcgttaga tacatctttc actgccttat
     5041 ctaacccctc atcacctccg tcccattcac caatattaat gaatataggt acattaccgt
     5101 ttatatcatg cttatctgta aataatttat gatatttacc caacatatca cgagctttta
     5161 aacgatcact aggttttatt ggtacctcta tcagttcaac atgttcatta tagactaatt
     5221 gtactttgcc actttgtgga ttttctttat attctccacg cttgactaca acttctttcg
     5281 tttctgtttc atcaccgact gccgcattcg ttagcacatg taataactct tttgcggtta
     5341 atacattctc atctataatc ttatcttttt gttcttgtat atattgcttg atgtgcggct
     5401 ttttcaataa cctacacgct gtcacatgtg cgctatttgc gttatatcct gcttttatgg
     5461 cactttgtgt tacattcagt gttctaatat actcattcac aaaacgtgct tgctttgcag
     5521 ttaactcact cattttatca cccccacaat tttatctaat atggtttcat accataatat
     5581 tacagattgt tctgaacaat ctaaggcact actaatatct tgataactaa gtccttgtat
     5641 aagggagtca aaaatataaa actctttatc ggtcgctaat ctgtcaacaa tcatttctat
     5701 gtgattcttt ataatatgat cattgacatt atcgtctgtc atcaattcgt cagaatcttc
     5761 atcacctatt aaaaagaaat catcagtatt tatttcatca tcgccccgtt aactagcttt
     5821 gaagtcttta gcacacttgc atataccggc tgtcgtgctg gcgagatact agcattgaag
     5881 tggtctgata ttgattttga aaacaacacg attagtatta ctaaaacata ttacaatcca
     5941 aataataaca agaagaaata tcagatactt ccccctaaaa ctgaaagttc tatcggtaag
     6001 atttccgttg acccaaatgt aataaaggtg ttgcgtgatt ataagataaa cgttcaaaat
     6061 aattggaaaa acgaattata taatgataac cattgtaatg aaaaaactat caatgtggat
     6121 acaaaccatt atgtcacgta ccagcatact caaaaatatc tctacgcact cattcagata
     6181 cacacattgc gcattgctca tagaatcagg tgtacacatt aaagaaatac aagaacgatt
     6241 acggcataaa gatatcaata ccactatgaa tatctatgct aaaatcacaa attcatacaa
     6301 aaaagacgcc tcccataagt ttagtcacca aatggaagac gtctcgtaat taaaatatat
     6361 ttgcgtgcat tctaatttat acttagaatg aatcatactc gtgcataatg taattttcta
     6421 gttagtcaaa actataaaca gttttacatc attcctggca tgccacccat gttaggttgg
     6481 tcattatttt tttctggaat tgatgctaca accgcttcag tcgttaagaa cattgctgca
     6541 acacttgcag catgttgtaa tgctgagcgt gttactttag ttggatcaac gataccttct
     6601 tctaacatat taacccactc gtttgtagca gcgttaaaac caacacccgg ctctgcgttt
     6661 ttcaaacgtt ctacaataac agaaccttct aatcctgcat tttcagcaat ttgacgaact
     6721 ggtgcagtta atgctttaag tacaatattt acacctgttt caatgtcacc ttcagcttca
     6781 atttcactta ctttttggta aacatttact aatgcagtac caccacctgc aacaatacct
     6841 tcttcaactg ctgcacgtgt agaatttaat gcatcttcaa tacgtaattt acgttcttta
     6901 agctctgttt cacttgctgc acctactttg ataactgcaa caccacctgc taatttagct
     6961 aagcgctctt gtaatttttc acgatcaaag tcagattcag tttcttcaat ttgagatttc
     7021 aattggctaa cacgtgcatc aatgctgttt tcgtcaccgt caccatcaac aacagtggta
     7081 ttatctttag ttacttctac tttacttgca gtacctaaca tatcaattga tgcatctttt
     7141 aaatctaagc ctaaatcatc agtaatcact tgcgcaccag ttaaaatagc taaatcttca
     7201 agcatcgctt ttctacgatc accaaaacca ggtgctttta ctgcaacagc tgtaaatgtg
     7261 ccacgcatac ggtttagcac gatatttgtt aatgcatcgc cttcaacttc atcagctaca
     7321 attaagattg gacgattaga ttgaaccact tgttctaata aaggtaagat atcttggaaa
     7381 gacgagattt tcttatctgt tactaaaatg tatgggcgtt ctaattcagc aaccatttta
     7441 tctgaatcag taaccatata cggtgattga taaccacgat caaattgcat accttcaacc
     7501 acttctagtt cagtgtttag tccatttgat tcttcaattg taatgacacc atcgttacct
     7561 actttttcca tagcttcaga aatataacgt ccaatttctt catctgctgc tgaaatcgca
     7621 cctacttgcg caatttcatt tttattttca actttttgag aattttcatg taacgcttca
     7681 acagcaactt taactgcttt gtcgatacct tgtcgtaaac caactgggtt cgcaccactt
     7741 gtaacatttt tcaagccttc ttgaatcatt gcttgagcta atactgttgc agttgtcgta
     7801 ccgtcaccag caatttcatt tgtcttattt gcgacttctt gaactagttt agcccccata
     7861 ttttcatatg gatcttctaa ttcgatttct ttagcaatcg tcacaccatc attcgtaatt
     7921 aaaggtgctg taaactcttt atctaataca acattacgtc ctttaggacc aatcgttact
     7981 ttaactgcat ttgcaagttg gtcaacacca cgtaacattg cttgacgtgc atcttcagag
     8041 aatttcaatt gtttaaccat aaatgataaa cctccatttg tttagttgtg tttcattttc
     8101 gttctattta atttatttat gaattaagtt ctgtattatt caataactgc taaaatatct
     8161 tcttcattta ataccagata tgtttcatta tctcgtttaa cttctgtacc agcatattgt
     8221 tggaacacga cacggtcccc ttctttcact tcaggagtca ctcttgtacc atcatttaat
     8281 aggcgtccag ttcctactgc aacgataacg ccttcgtttg atttttcttt agcactatca
     8341 gttaaaacaa taccactttt agttgtttgt tcttgttctt ttttctcaat aatcacacga
     8401 tttccaattg gttttagcat gattgttcct ccttaaaaaa cctaaagttt agcacttaac
     8461 attaaagagt gctaacatac atttataata atcaaatttg gtcaaaattt caa
//
LOCUS       DALAGF010000114         8459 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.114, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000114 DALAGF010000000
VERSION     DALAGF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8459
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.114"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(261..587)
                     /locus_tag="O2692_002346"
     CDS             complement(261..587)
                     /locus_tag="O2692_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCZ4299953.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSANVEMFVEYV
                     KQHMIV"
     gene            complement(651..1394)
                     /locus_tag="O2692_002347"
     CDS             complement(651..1394)
                     /locus_tag="O2692_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCZ4299954.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDSLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELLRNLKCYLNMCQLSLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVNLNNK"
     gene            complement(1420..3375)
                     /locus_tag="O2692_002348"
     CDS             complement(1420..3375)
                     /locus_tag="O2692_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4299955.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDL
                     KRNYVTIIMLNDLYQIDGNDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSAERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPKELNTRYDKEIDHLIDY
                     FILPLRQIEQVSIARSKIICDVDLINENNDLKEKITMSDINKVSELLTNYTKFRGLIM
                     HMSIISESSKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
     gene            3985..4788
                     /locus_tag="O2692_002349"
     CDS             3985..4788
                     /locus_tag="O2692_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ4299956.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNNYNNGYSYNNYSRYN
                     NYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYT
                     SGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQ
                     GAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            complement(4805..5047)
                     /locus_tag="O2692_002350"
     CDS             complement(4805..5047)
                     /locus_tag="O2692_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299957.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
     gene            complement(5221..6621)
                     /gene="nhaC"
                     /locus_tag="O2692_002351"
     CDS             complement(5221..6621)
                     /gene="nhaC"
                     /locus_tag="O2692_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCZ4299958.1"
                     /translation="MMKRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNESMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVEEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            complement(6716..7798)
                     /locus_tag="O2692_002352"
     CDS             complement(6716..7798)
                     /locus_tag="O2692_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HCZ4299959.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
     gene            8046..>8459
                     /locus_tag="O2692_002353"
     CDS             8046..>8459
                     /locus_tag="O2692_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HCZ4299960.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDIDFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNGQEYIIPLTIR"
BASE COUNT         2899 a         1504 c         1075 g         2981 t
ORIGIN      
        1 taatttgatg tttaagatat tttgccaaat tgaaaattat tagtctgaat attttgtatc
       61 ttaactgata tgtaaacttt taaaatttta atctatatca acgtgtttga tttgatattt
      121 tttatattaa ttggatggct tgctcacaaa atttataaaa tataaaaaaa gaagagcttt
      181 aaatcataac gcatgccatt tatctttaaa tgcaatgagt tatcattcaa actcttctta
      241 tcaacctaca tacattgaca ttatactatc atatgttgtt ttacatattc aacaaacatt
      301 tcaacgttag cacttttaat atcatgcatt gggacattac caatattaaa tttgaacatt
      361 atagtttgac catcataatc tatcttctct atttcattat aactaatact tctataataa
      421 aattggccat tcatatctac attcataatt aatctttcat ttgttgctat aaatgcccct
      481 tcaaattcat tctcaccttg aacttgatac tcaattattc ctagcactga cggacccttt
      541 ttttcagttg gaaataaatc atttggattc acattatcta aaatcataat atccctccca
      601 cttaaaacta taaactattc ttcataggta tatgaaatac aagtgattaa ctatttatta
      661 ttaagattaa cttgtattcc cttttcaaga taattataac atgcttgaat aagcatagtg
      721 actttagaaa acttttctcg cttgattgtt acaataacac gcctttcatc taattcacat
      781 ctctcttttt taactaatcc cttcaaaatt aaacacttta aaatcgggct aaccgcaaat
      841 atcgggtgat ggaattcacc ctgtaattct ttaaccgtca attgaccttt atgcagattt
      901 aatattccta aaacgtataa ctcttctaat gataattgac acatatttaa ataacacttt
      961 agattccgca ataattcttg tgacgacaat aaatacgata catagtcaca actaaaatca
     1021 aatgtatatt gaccaaaata acgttcaata tagtcactaa gctcattatt catatattct
     1081 attttatcta tatgcatttt attaatcgaa atagtcaatt tacgttgatc cctttgatct
     1141 cttcctttca atagccattg atgattgttt aaataactta acatttgaag aagggccatc
     1201 ttagattgga tttctttcat ttttaataaa ctatctattg aagcgctatt ggaatcagaa
     1261 ttatcatatt taactaatag atttaatagt ttcacttgag tcaaagtgag atgaaatgaa
     1321 tatttcaaca tatcattaat gactttcaac attagtatgt gtgcagttaa acgcttaact
     1381 ttaaatttag acatctttaa aacctctctt aaaccatgcc tatatctcaa gatgatattt
     1441 caaatgaaca atactattgc ttgagaccat taatgaatga tcataaatat ttctttctat
     1501 aaaattagct ttccaataac tgtgttgttg cataatatca ttcacaagta caccattttc
     1561 ggaagtatga ttatctttat ctatacttaa aacaatttgt ttagttttag catggctaaa
     1621 ttgttgaaga cccttacacg ataaacgtat agcgtctgaa ttctcattta acaatgcggc
     1681 tggcaaacaa atgacacatt gtacttcatg tttgaacttc gttacaatca tcgtgtcatt
     1741 ttgataaata acaatccctc gtaattgatt aagtatattt atgataaaaa tgaatattga
     1801 taatctagtt aaatttattt ctttgctcga ttcacttata attgacatgt gcataataag
     1861 tcctcgaaat tttgtataat ttgtcaaaag ttcagaaacc ttattaatat cagacattgt
     1921 gattttttct tttaagtcat tattctcatt aatcaaatct acatcacata taattttaga
     1981 acgtgcaata gacacctgtt ctatttgtct tagcggtaag ataaaatagt ctattagatg
     2041 atcaatttct ttatcatagc gcgtattcaa ctcttttggt tccagatgat atattaattc
     2101 aaatactcct atataattat ctattaaacg tttcagtttt tgaattgcag tagtatttaa
     2161 gtcgttaatt aaaaatgcaa tcttacatct attactctca aaagtcatat taaaaattgt
     2221 agctactagc ttttctatct tgcctctttc agcactcgtt aacaattcct tatattcatg
     2281 tttaatacga aacactacat ttacattgta attcactatc ttttgaagta ataaagtgat
     2341 ttgttcaatc gatagctcat tgcttgtgtc gattgtaaca attatatgca agttttcagg
     2401 attaacacct aatctttgaa tgatttgttt aatagtataa taatccatcc aataaaaatt
     2461 acttccttta atataaatgt ttttaggttg atacatttga ctcctttttg taggctcaaa
     2521 aggtatatca atctcgcgca tacttgaaga actttgatta gtatcatcaa ataattcaat
     2581 tatattttta tcaatttcaa ctaattggga atggttaatt gaatgagatg ttggactata
     2641 tctttttcta attaatttag gagtgttacc atacgtttct ttaaagaggt gtataaaacg
     2701 agaataatga ttaaaaccat gactacttgc gatttcttca ataggcttct tagaagttaa
     2761 aatatcaatt aagcaatgct ccagtctaat atgatttaaa tattgaataa aattactata
     2821 aggtgtcgct ttaaacatgt cacttaaagc tttgtttgta atactaactt gattaatgac
     2881 atctttccta tttatctttt tatggtggtt gtttgttaaa taatcgtgca cttcttcggc
     2941 tactaaatga cgactaccat cgtatgtatt taatgacatt aattcaacac acatgtaatt
     3001 aataatctta tcattagcat tataagactg ttgtttaatt ttgctgtaga ttaaatactt
     3061 aatcaagatt ctaaaagtgc tagcaacctc atttgttaat atcttgccac gcatcatata
     3121 gtttgcattc ataaacttca aaaatgtctt tgcacttact tcaacaacac aacatacact
     3181 atcatcatta ccatcaattt gatacaaatc attcaacata ataatggtga cataatttct
     3241 ttttagatca tattgttgta gattgattga aactacacct gtaccttgta accaatatat
     3301 aatttttaag ttcgcttcct tcacactact cattttcata cttttataaa tatgtagtga
     3361 ataattatct gtcatcttac atcctcacat atacaaatat attgatttac tcctgtttgt
     3421 ctttctcaaa tcaattgtca acatctaata ttcactacca attatatcgc cacatgtttt
     3481 ttagcagtaa caaaacataa atcgattata tcaaataata cttttattaa aacatttttt
     3541 tatgctaaat taggatattt tatccattaa atatacattt tcatccaaat accaaagctt
     3601 tcataatcat agaaacttga tcgttcgtgt cgaattttta tgcattttct gattaaaagt
     3661 cctatattat gcacaaaata tacatttaga attttcagta atattacaaa tttgtaacag
     3721 acttattttc gaaacattca gttttcacaa agaatgccgt agttataagt tttgcaaaat
     3781 gcaaatttct atacttttaa aattctattg tgtcaagata ttagcaaaaa attacaaaac
     3841 gttaacaaag cacacaagga cgctaatttc tgttatagtc tttcttgtcg tttaattacg
     3901 agagataact tataacatta attttgtcac acggacatta cgaaaatatt ttagatttaa
     3961 aatttttagg aggatatttt taaaatgaag aaaatcgcta cagctactat cgcaactgca
     4021 ggattcgcta caatcgcaat tgcatcagga aatcaagctc atgcttctga gcaagataac
     4081 tacggttata atccaaacga cccaacatca tatagctata cttacactat tgatgcacaa
     4141 ggtaactacc attacacatg gaaaggtaac tggcatccaa gtcaattaaa ccaagataat
     4201 ggctactaca gctattacta ctacaatggt tacaataact ataacaatta caacaacggt
     4261 tatagctaca ataactacag ccgttacaac aactactcaa ataataatca atcatataac
     4321 tacaataact ataatagtta caacacaaac agctaccgta ctggtggttt aggtgcaagc
     4381 tacagcactt caagcaacaa tgttcaagta actacaacta tggctccatc atcaaatggc
     4441 cgttcaatct caagtggtta tacttcagga cgtaacttat acacttctgg tcaatgtaca
     4501 tactacgtat ttgatcgtgt aggtggtaaa atcggttcaa cttggggcaa tgcaagtaac
     4561 tgggctaacg cagctgcaag agctggttac acagtgaaca atacaccaaa agctggtgca
     4621 attatgcaaa caactcaagg tgcatacggt cacgttgcat acgttgaaag tgttaacagc
     4681 aatggttcag taagagtttc agaaatgaac tatggttatg gcccaggtgt tgtaacttca
     4741 cgtacaatct cagctagcca agctgctggt tataacttca ttcactaatt agtgatggct
     4801 atgtttacgc caacaaggtt tcaagagcct tgttggcttt tttaatttct atgaaaaaaa
     4861 tcgaattggt aaaaatctca aaatttcgat tttcaccaat ccgattcact ctgtaataaa
     4921 gaataaacaa catacagcca ttgacacacc caaatttagg catgtcaaat cggctataca
     4981 ttgataaatt tttagttaaa gtacttacaa atatacttgc cttcaaattc tcaattaatt
     5041 tagtcattac aaatcatgac ataaaactta cattacatat atcaacatgt tccgctacaa
     5101 aacatattca gtatatgtat agaggatatt tattctaagt taaaaccttt tcaaattata
     5161 accgctacaa ttataaatga ttaaagtgtt tcaacatatt atatatattc cacgctcaca
     5221 ttaagttaaa cgtgaattcg atgacttttt aatacctatc cctgtaaaac catagataat
     5281 tgctataatt gcgcataaat aacatggtac tgtccatatg aaaaattctt caacagagac
     5341 atgaagttga ttcgtatagt aaatacctga tgttccccat ggtattaaag gtaagaccat
     5401 cgtacttgag tcttcaagtg ttcttgataa aacggaacga gagacttggt atttttcgaa
     5461 caaatctttc attaacacac cgaccataat aattacaatc gaagcaacac ctgcagcgaa
     5521 tacaagcgca atacaacaaa taacagtaat acatattaaa cttcctacag aatggatgcc
     5581 tttagaaata gtagttaata agacttctaa acatcctgct ttttctacaa tacctgcaaa
     5641 tgcatatccg caaaatatcg ttactaatat ttgggtcata ctcatcatac caccctgttc
     5701 taacaagctt ttcacactgg atgaaatatg agactgatgt atcattgatt cgttaaaacc
     5761 actaaatgtt gctttgaaac catctgtcat tttgaaatga tgattaaaag tccccactat
     5821 aatggcagaa aagcttgata ttagcatagc tggcactgta gccattttaa atagcaaaca
     5881 aacaatgatc acaattaagg gaacccagac ccatatgtta atttgataaa tctgtgcaag
     5941 ctctgataac aaagtttgaa tctgtttatc atttgaatgc cctttaaatt gaaatccagc
     6001 aataaaccat actaataaac ctatgattga tgcaggtatc gtcgtccaca tcatcgaatg
     6061 tatatgttta aatatattaa ctttagtaac aagcgccgct aaattagttg tatctgataa
     6121 tggtgacatt ttatcgccaa acacagcccc tgctataata gcacccgctg ccatccctgg
     6181 aggaatcccc aattgattac caatagaaat aagtgcaatc cctgcagttg atgcagagcc
     6241 ccatgctgta ccagttgcta cagatgtaac agcacttata aaaaaggctg atattaaaaa
     6301 atagcttgga ttcaataaat ctaagccata ataaatcaag gctggcactg tgccagaaaa
     6361 catccaactg cctactataa ttcctaccgc tagtataatt aaaattgcag gcattgcagt
     6421 atttaaacgt tctgcaatgc ctttttctaa atcttgccat gttaagccta cacgttttgc
     6481 aatccatgtg gcatatgctg aggcaataat taataatact tgaattggaa tatcaaaaaa
     6541 cacaaagcct gttacaacaa caataaccat tacaatcatt gtcgaaattg attctaaaaa
     6601 ggtgggtttt cttttcatca tctatcgtcc ccttcgctta ttgatgagaa acctcttttc
     6661 ttcaatttat tgcttttgca taatgctata tccttttcta tcttcaagcc atcatttatt
     6721 ttaaatattt ttctaaatca agcttatcta aaccaatttc ttcaactgtt aagccttcct
     6781 caaagaagtc tctctctaaa atggttgatg caattacaat tactgcatct atatttggtg
     6841 tcggtacatc aataacacga cctaagcttg accatagtac taaaccatat gcaatatctt
     6901 cagttaaata tctgctttct acatgatttg gtcctggaat ttgtgaaaat actgggcttg
     6961 tattaaacaa acgatttaat ggttcatctt ctttatcacg ttctaaataa ccacgttcaa
     7021 tacgtgattc tttagctgtt gataattcaa aacctaatct acggcctaaa ttcaaacgtt
     7081 ctaattcgat tgcatgaagt aatctaactg tatgtttagt aattccttct ttatataaag
     7141 cgaactcgcc agcataatca atgcgaccga cattcaataa tgttggtcct ggatgcactt
     7201 caggattacc attttcaaga tttgttttaa ttaagctttc ctcttttact aaatgatcat
     7261 aaatacttga aactttgtca taacaatcat ttagacagct tctatcatat gttgaaaaga
     7321 agatacgacg tacatttaga gataaatcaa ctgctgcatt ttcaaaatcg acacgcgtac
     7381 catacgtcaa cgtattagct tccgctagtt gtggttttgt ttcaatatgt ctatcttcta
     7441 aaacattcat aaaacgaatt gaccccattg ctgcagccat gttgaagaat atcaactgat
     7501 tatcagttac atgctctgcc attacatcag cataatactc tatgtatgaa gatggaataa
     7561 tcacttgaac aatttcagca tcttttaaaa catattccat atcatcacta atatcagtga
     7621 attttacgaa acgttcatca ccttcattat taaaatcaaa tccgcccttt tcgattgcgt
     7681 tttgaaactt acttatagat tgattacgac aatataattt aacatcgtgg cctttgctca
     7741 ccatatctac tgctgccgta actgcgccat ttcctgatcc tacaattgct attttcataa
     7801 acaaccctct ttctatcttt ggttaaaatt aaaaacaaat tatacgtaag acaatataaa
     7861 aatgatgcga attcattacg catcattata aatgttaagt atatataacc ttaaattaac
     7921 ctatttaaac gccgacgtcc accaattatt tttattttta ataattgttt aataaacatg
     7981 tttatttttg attatatttt gttatagtta aagataatat tacaattttc aaaggagtgt
     8041 ctaatatgac aactgaaatg aacgacgtaa caaatcatca accgaatcgc aaatccaatg
     8101 atgagaacat tatggcgatg cttatctatt tattaagttt attcacatct attattggtc
     8161 cactgataat atggttattg aaaaaagatg aatcgaaact agttgatcgt gctggaaaaa
     8221 attatcttaa ctatacgatt tcttatatta tttggtctat cgttttagtc gttatcactt
     8281 taataggcgt tttcctaatt gctacagata ttgatttcat tattattata ggtttcatca
     8341 ttacgtttat cggtatttta tccatattcg ctttctcaat attatctttt gtattcacaa
     8401 ttattgcgtg tgtcaaatat tataatggcc aagaatatat aataccttta acaattcga
//
LOCUS       DALAGF010000115         8436 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.115, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000115 DALAGF010000000
VERSION     DALAGF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8436
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.115"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(60..325)
                     /locus_tag="O2692_002354"
                     /pseudo
     CDS             complement(60..325)
                     /locus_tag="O2692_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            complement(325..576)
                     /locus_tag="O2692_002355"
     CDS             complement(325..576)
                     /locus_tag="O2692_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCZ4299961.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSVMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(741..842)
                     /locus_tag="O2692_002356"
     CDS             complement(741..842)
                     /locus_tag="O2692_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299962.1"
                     /translation="MLRSVSDTNLLGQYEKAISISDMIEIVFLMYEV"
     gene            complement(849..1448)
                     /locus_tag="O2692_002357"
     CDS             complement(849..1448)
                     /locus_tag="O2692_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCZ4299963.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVVYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(1467..1826)
                     /locus_tag="O2692_002358"
     CDS             complement(1467..1826)
                     /locus_tag="O2692_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCZ4299964.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDEVHYYAYDFSDKRVSYKQDFDSR
                     RYYQQHDADYHEENMTN"
     gene            2081..3331
                     /gene="fmhA"
                     /locus_tag="O2692_002359"
     CDS             2081..3331
                     /gene="fmhA"
                     /locus_tag="O2692_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HCZ4299965.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            complement(3428..4159)
                     /locus_tag="O2692_002360"
     CDS             complement(3428..4159)
                     /locus_tag="O2692_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCZ4299966.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     GAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(4156..4875)
                     /locus_tag="O2692_002361"
     CDS             complement(4156..4875)
                     /locus_tag="O2692_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCZ4299967.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(4856..5635)
                     /locus_tag="O2692_002362"
     CDS             complement(4856..5635)
                     /locus_tag="O2692_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCZ4299968.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(5756..7195)
                     /gene="mdeA"
                     /locus_tag="O2692_002363"
     CDS             complement(5756..7195)
                     /gene="mdeA"
                     /locus_tag="O2692_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HCZ4299969.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVVSFFCSFMLQGKKKEVDSRQ"
     gene            7369..7473
                     /locus_tag="O2692_002364"
     CDS             7369..7473
                     /locus_tag="O2692_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCZ4299970.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(7627..8313)
                     /locus_tag="O2692_002365"
     CDS             complement(7627..8313)
                     /locus_tag="O2692_002365"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCZ4299971.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
BASE COUNT         2815 a         1554 c         1166 g         2901 t
ORIGIN      
        1 ttgccttttc atattgtcct aaaataactt accctatttt tatagtgtga cttttagatt
       61 taataatggt atttacatga tgctattttt atatgattgt catctactgt ataaaccaat
      121 ctatgttcgt gattgatacg tctactataa tacccagtca gattcgattt taacttttca
      181 ggcttaccta taccttccaa tgcaccattt ctgtcaatac ttttaagtaa ctcattaatc
      241 ttcttcacca ttttttatcg ttctgctgaa aatacttata atcttcaaag gcttgaggcg
      301 aaaacgtaat atttaaccta gccattatac atctatatct ttcgttatag ttttcccacg
      361 ttctagatct gcaattgatt gagctaagtg ttcagcatta tttgggttct gttggaggta
      421 aagtgtttcc atcatggagt tataatctga ttctgacatg attactacgt ttttatcatc
      481 agtagatgtt acagttacca taacactatc atcatttact tttgtcataa gtgcctttaa
      541 attctgtcga gcgtatgaat aatttttaat aatcatattt atcgcctcct tttaacacac
      601 ctcaattgta caaatatctg tacaataaaa caagcgacac agaaaaaaac tactaatcaa
      661 gatttcgcac ggggaagaat caatacctat cctgtactga ttccctacta aacaatgttt
      721 acatgataat ttccacctat ttagacttca tacattaaaa agacaatctc tatcatatcg
      781 ctaatagaaa ttgccttttc gtattgtcct aataaattgg tatcacttac tgatctcaac
      841 aattaagact atttgatttt atcttttaat aacttctcat aactttcata atcatatgga
      901 tcttcaactt tctcgccatt aataaatgca gttggtgtcg tttttatatg attatctttc
      961 gctatttttt tatctttctc tgcagctttc caagacttgc tatcttttgt cttgtaatct
     1021 tttataattt tattttccgt ctctttatct aaatgcagtt gtttaatatg tttatctaat
     1081 agttcttttg ttaaccattc tttattttca tcttgctggg cagcaaataa ttgcttttga
     1141 aaatctaaaa atgatttagg tgcatacatc aatactgcat gactcgcacg cgaaccaaca
     1201 attgagtctt tacctaagaa agctaaattg acaaattggt attccacttt gtgattatct
     1261 atataatttt tacgcaactt tggcatgact ttttcatcta attctttaca ataaggacat
     1321 ttatagtcgc catatacgac aactaatggt ttgccgtttt tcgaagatgt cgttgctgat
     1381 tcttttttac cgcaagctgt taaaattaac atgctcacta taaataatgt tagtaatttt
     1441 ttagtcataa taactcctat tcatatctag ttcgtcatat tttcttcatg ataatccgca
     1501 tcatgttgtt gataatatcg tctcgaatca aaatcttgct tatatgatac acgtttatca
     1561 ctaaaatcat atgcataata atgcacttca tcattactca aaggtttata agctaaaatg
     1621 ataattttat ccttatcata gacataaata ttagcatctt tgcgttcaaa atgatcaaca
     1681 cgatcaccgt tgcctttttt ggcaagttgt tcttgttttt gattttgaat tttaactgct
     1741 tcatctattt ctttcacata tttatcatta ccacatcctg atataaagac tagtaataat
     1801 aataccgtcg ccacaaatcg cttcaatgct cccactccta ctatcaactt ccaactaact
     1861 ttcaatataa ttaatactat acaccgtcaa acattaatgc gctacaacta ctaccaatgc
     1921 atttaatata aatatccaaa gaaataacag ttatgtatag tcaaagaaaa atatatttca
     1981 ttttctatgt gtatttgtaa tcagtttttg ataaaataaa aaaatagcat tattaataaa
     2041 ttcaaaaatt atagttcaat tcagagaggg atgggataaa atgaactttg taacgttgac
     2101 ttcggatgag ttcaatgcgt ttacaacaaa gcatttttca cattacacac aatcagctat
     2161 tcattacaat catagagttg atttaaaagg cgatgtgcat cttgtagggg ttaaagatga
     2221 caatggtcaa gtgattgcag gatgcttatt gacagaagca cgcacactta aatttttcaa
     2281 atatttttat acacatcgcg ggccagtgat ggattataca aatcaatcat tagtagcatt
     2341 tttctttaaa gcattaacgt catatttaaa gaaacacaat tgtttatatg tccttgtaga
     2401 tccatattta attgaaaatt tacgcaatgc agacggtgaa attgttaaat cttatgataa
     2461 ccgagcattt gttagaacaa tggataaatt aggttataaa caccaaggtt tccctgtagg
     2521 ttatgattca atgagccaaa tccgttggct gtcagtgtta gatttaaaag ataagactga
     2581 agaccaactt ttaaaagaaa tggattatca aacgagacgt aatattaaaa aaacatatga
     2641 tattggtgtc aaaactaaaa cgttaacgat tgatgaaacg caaacttttt tcgacttatt
     2701 ccatatggct gaggaaaagc acggtttcaa attccgtgag ttaccatact ttgaagaaat
     2761 gcaaaagtta tacgatgacc acgccatgtt aaagttggcg tatattgatt taaacgagta
     2821 tttaaaaacg ttacaattaa agcaacaaca attaacagct gaactctcag gtgttgaaga
     2881 agcattggaa gaaagtccta attcaaagaa aaataaaacg aaacgcacac agctagaaca
     2941 acagctaaat agcaataagc gtaaaatcga caatacaata gaacaaattg aacaagatgg
     3001 tgcagtctta aacttagctt ctgctttatt tatctacaat gagcatgaag tttattactt
     3061 atctagtggt tcaaacccta aatataatgc ttatatgggt gcctaccatt tgcagtggga
     3121 aatgattaag tttgctaaag cgcatcatat tgatcgctat aatttttatg gtattaccgg
     3181 tgacttttca gagtcatcag aagactatgg cgtccaacaa ttcaaaaaag gttttaacgc
     3241 acatgtagaa gagtatatcg gtgactttat taagcctatc aaaccattac tatataaggt
     3301 acaaacgtac ttaaatcata aacgtcgtta aaatatcgct ccaagattat gacaacataa
     3361 caaagaacca cgcatcatgt atatggcttc atcgccatac taaaacatga tacgtggttc
     3421 tatttgatta ttcttcattt ataacattta agaaacgtct taattcttct gttttcggac
     3481 gattgaacat ctcttctgga gccccttgtt ctccgatcat gccttcatga ataaatacaa
     3541 tgttattaga tacttcttta gcaaaacgca tttcatgtgt cacaatgacc attgtcatgc
     3601 cttcattagc caaatcttta ataaccttta aaacatcatt cacaagttca ggatcaagtg
     3661 cagatgttgg ttcatcaaac aacatcactt tagggttcat tgctagtgct cttgcaatag
     3721 caacacgttg ttgttgacca cctgataatg catgtggacg ttgatctttg acatgtgtta
     3781 aaccaacttt ctcaagtaac tcaagtgatt tcccacgtgc ctcatccttt ttcaactttt
     3841 taactgtgat aagaccttcc attacatttt ctaatgccgt cttatgcgga aaaaggttat
     3901 aactttgaaa taccatacca gactgtttac gaacttctat ttgtgatttt ttatctttag
     3961 atgtatatgt tttgccgtta acataaactg taccttcagt tggaatttct aatgcattaa
     4021 tcatacgtaa caatgttgtt ttacctgaac cagatcgacc gattaaggtt acaacctcac
     4081 cttgttcaac agataaatca atacctttga tgacttcaac atcattaaat gatttatgga
     4141 tattgttcaa ttgaatcatg agcgataccc tctttcaata taagattcat agataccttg
     4201 gataattgaa ataataaagc ataccaccca gtacattagc gcaactaaca cataaattgt
     4261 taaatattca tatgttgtag acgcaacttc ttgagctttt ctaaacattt cagccactaa
     4321 aataaatcct aataatgatg tatctttgat taaacttaaa aatgtattac ctagtgcagg
     4381 aatcgacact cgaattgcct gcggtaaaat gatacgttgt atcgtttgac catacgtcat
     4441 accgatggaa tatgcagctt ctgtttgtcc tttcggtata gaaataatac cgccacgaat
     4501 aatttccgaa gcatacgcac ctacatttaa tgataaacca ataatagcag ccactacagg
     4561 tgccaatgtc cattggttgt cagcgtcatt tgttaataat ctacctaatt ctggaatacc
     4621 ataaaatatg ataaatagtt gtactatcat tggtgttcct cgaataatag atacatagac
     4681 acgtgaaata cttcttaaaa ttttactcgt tgaaattcgc attaatgctg taaataatgc
     4741 aataatcaat cctaaaacaa atgtaactaa tgtaatagga attgaatatt tgaccaaacc
     4801 ttctagcata ggtccgaaag cttgttttgc agcatctagc gcatgttgtt gttcgctatt
     4861 tagatttaga aacatcttga ccaaaccatt tcttacctat tttagctaat tcaccgtttt
     4921 cctcgatttt tttcaagcca tcattgaatt tttgaactgt ttcatcatct gcttttttag
     4981 aaaatgcaaa tgcagatcta ctttgttcag cattaccttt gattgctttg atcttagcat
     5041 taggtttttg ttttttataa tccaagtatg acagactatc attaaatgta ccatcaacac
     5101 gcttagacaa taataaatcc attgattggt taaagccatc aacttttgta atatcagcac
     5161 ctttatcctt agctaattta ccataattag atgtgaatgt ttgtgctaac ttcttacctt
     5221 taacatcatc aaaatcttta atatcttttt cattttcacg aataactaaa acagcacttg
     5281 agaatgtgta aggcttagaa aatttatatt tcttttctct atcaggatta atacctactt
     5341 ggttcgcgat tacatcaaaa cgccctgcgt ctaaacctgc aaacatagaa tcccaagaag
     5401 tttcattaaa tttaagtttt aaaccttctt ctttagccac tgctttaata acatcaatat
     5461 cgtaaccagt taatttacca tctttatcgt ggaaactaaa tggtgcataa gtcccctcag
     5521 tcccaacatt aattgtcttg ctatctttac tagcttcctt gtctttcgac gaattgtttc
     5581 cgcatgcagc caatatgaaa acgaaagcta tcatcacaaa taaaagtctt ttcatagaat
     5641 atatctcctt attcttatta ttctaatcgg aataaaaata ttttaatctg aaatgccgga
     5701 taagtcaata tgttgaactt ttgctaaaaa gctttatata gctagcaact tatatttatt
     5761 gacgagaatc aacttctttt ttcttacctt gtaacataaa tgaacaaaag aaactaacaa
     5821 ctgtaatcaa taatgcaaat aaaaatccag cgtgataacc atgcaacatc gtatcaactt
     5881 taatttgatt catcatatca attttgttca tgccattata gtctgacata gttggagaaa
     5941 agtttttgct tgcatgggat aaaatagtaa ttaatgcagc tgttccgata gaaccagcaa
     6001 tttgttgtac tgtgtttgtc atagatgaac catgtgcatt catttctggc tttaattgat
     6061 tcatcgtatg ggtcattact ggaattaatc ctaacgcgat accaaccatt cgaatcgcat
     6121 aaactgttgc caacatgatt gttgatgttt gttcatccat tacaacaaaa taagccgtag
     6181 taataacaac aatcgccata cctactaaag caagatttct aggaccaaac ttttcatata
     6241 aagcacccga agtcatcgac ataaatgcca taacaattgc tcctggtaat aaagttaatc
     6301 cagaatctaa agctgaacga tgcaataaat tttgcacaaa gataggtaaa atcgtttccg
     6361 aaccaatcat cgataccatt gtgacaccca taataatgat accaactgta aattgtttat
     6421 ttttaaatac agcaaaattt aataatggtg ttgatagccg atattgacgt aatacaaata
     6481 atgcgactaa aacaacgcct ccaatcatag atactaatac gataggatta tcaaaacctt
     6541 tttctgaaat tgaactcgtt ccatacaata acccgccgaa acctagtaca gaaaaaatga
     6601 cagaaataaa atcaataggt actttaattg tagttccaac attttttatc gttttaaatc
     6661 caattaaaaa tgtcacagct gcaattggag cgacaacatg gaaaagcgat ctccagttaa
     6721 aatattcaac aaggtaacct gaaagagtag gtccaatagc tggtgctaaa ccaattacca
     6781 aaccaaatat ccccatatat ttaccgcgtt catgtggttg gaaaacatcc aaaatcgttg
     6841 tcatcatcaa tggcatcata atacctgcgc ctaacgcctg aataattcta gcaactaata
     6901 atgttgcaaa gtgcggactc aagccgccga gtaatgtacc taagaaaaag atagaaatac
     6961 ccactaaaaa cacttgtctt gttgtataac gttgtataac aagcgcggac aaaggaataa
     7021 caatcccatt agtcaataaa aaagctgttg tcagccattg aacttgtgta tatgaaattt
     7081 caaaatcttt cataatactt ggtaaagccg tcactaatag tgtttcattt aataatccaa
     7141 aaaatccacc catcagcata gtaataatca tcgttaattt ttgtttcttc gacatttaaa
     7201 gcttccccct caccatctaa aaaacgacac acatttttag atatccggta attataccca
     7261 ttttccaaaa aattagcacg actttattac aatttcttta aaacgtaatc attactattt
     7321 acatatgagt agttatcggt cataatatat ttacaaggag ggatagagat gaaattagat
     7381 ttacagactg ctcgtcgtaa tttaaatagc cctaacatta aaacaagaaa gcgtgcttta
     7441 aagattatta agcaacataa aagagcgaaa taattgtttt aatttgacta tattgtatgt
     7501 ctaactatat tttcgagtat ccctagctcg ttaatataaa ctctatactt aactatttcc
     7561 ttacattact tcacatattt aagagctgca tatacactca ctccttgtgt acatgcagct
     7621 ctttttttat aagtagtatt tatctataac ttctaaatca tccgttaatt cataaacaag
     7681 cggtgcacct gttttaattt cataattaat gatatcttca tctgacacat cttcaagata
     7741 tttaatcaat gcgcgaattg aatttccgtg tgcagaaact aataccgttt gaccatctag
     7801 caaatattgt gaaatatgat ctgtccaaaa tggtatcact cgaactaaag tatctttcag
     7861 actttcagaa taaggcatca tacgtttatc taaatgatta tatcgacgat cagctaagta
     7921 agcttcacgt tgttcttcgg tttcagcagg tggtttcaca tcataagaac gacgccaaat
     7981 atgtacttgt tcttctccaa attcttttct agcatcatct ttatttaagc cttgcaatcc
     8041 accatagtgg cgttcattta aacgccagct tttatataca ggaatccatt gttgtttaga
     8101 ttcagttaaa atataatgcg ttgtatctaa agcacgtgtt aataacgatg taaaagctac
     8161 atcgatggca atgttatttt ctcttacttt ttcacctgct ctagtcgctt cattaatacc
     8221 ttgttcagat aaattaacat cttcccatcc agtaaataag tttttagcat tccactcgct
     8281 ttgtccatga cgacataaaa ttaattttgg cataacttat aacctctttc ctttaatctt
     8341 taggacgtta tttcagggat attttaagat gatttaatct tagtaatttt cttttcaata
     8401 tattttaaaa ttcaatgatt aaatcaatta tcacta
//
LOCUS       DALAGF010000116         8400 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.116, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000116 DALAGF010000000
VERSION     DALAGF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.116"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            48..1571
                     /locus_tag="O2692_002366"
     CDS             48..1571
                     /locus_tag="O2692_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299972.1"
                     /translation="MSKKLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFNNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKD
                     FEKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYNDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            1639..2805
                     /locus_tag="O2692_002367"
     CDS             1639..2805
                     /locus_tag="O2692_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4299973.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRTLITWFYTMLWSIVGVVWIVFSIPNIFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            2802..3722
                     /locus_tag="O2692_002368"
     CDS             2802..3722
                     /locus_tag="O2692_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4299974.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            3736..4395
                     /locus_tag="O2692_002369"
     CDS             3736..4395
                     /locus_tag="O2692_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4299975.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWTYTY
                     Y"
     gene            complement(4447..5397)
                     /gene="lytM"
                     /locus_tag="O2692_002370"
     CDS             complement(4447..5397)
                     /gene="lytM"
                     /locus_tag="O2692_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HCZ4299976.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(5723..6715)
                     /locus_tag="O2692_002371"
     CDS             complement(5723..6715)
                     /locus_tag="O2692_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCZ4299977.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWKSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHTVEVSFKEGE
                     VVIKDNPIGVLTNHPDLNWHYSNLRQYINISPYPATANLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNELVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            6949..8325
                     /locus_tag="O2692_002372"
     CDS             6949..8325
                     /locus_tag="O2692_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4299978.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAS
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSIICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
BASE COUNT         2816 a         1430 c         1358 g         2796 t
ORIGIN      
        1 gtattgttct gatatgatta actcataaat aattgggagg aagcaaaatg tctaaaaagt
       61 taaaaattat aattcctatt attattgtct tactattaat aggtggaatc gcatggggag
      121 tttatgcatt ttttgctaat acaccgaaaa atacatacct aaaaagtgaa caacaaacag
      181 caaaaatgta taaagattat tttaatgacc gttttgaaaa tgaagtgaag ttccaagaaa
      241 agatgaaaga taattcgttt ttatcttcat tagaattaag cgcagatgca tcagatgaaa
      301 ttgttaaagg gcttggcatt cctaaatctg ttgttaatgc atcgaaaatt aaaatgtcat
      361 atggacatga tcctaaaaaa gagaaatcaa tgattaatct tgaaccaaca atcgcagact
      421 ctgcattagg gaaattccag ttagctgcag ataaagataa gcattatttc gaatcaccat
      481 tatttaaagg gaaatatagt gttaataatt ctgatttatt atcaacttat tcaaaactta
      541 caggtgaaga tgaagaaaca gcaaaagaaa atggtattac aaaccaacaa ctaaatttaa
      601 atactctttt taataatgct caagcacaac aaagtgacta cagcaaaatt gccgaaaaat
      661 attccgaact tattgtcgac aaattagatg acgataattt tgataaaggt aaaaaagaag
      721 aaattaaggt taatggtgaa aagtacaaag ttagacctgt cacgttaaca cttagcagag
      781 ctgacactaa aaaaattaca ttagctgtat tagaagaagc taaaaaggat aaagacctta
      841 aaaaattaat ggaagaacaa ggtgctacaa aagactttga aaaagacatc aaaaaagcaa
      901 ttgacgatgt caaagaaact aaaaaggatg aatttgctaa aattcaatct aaaatttata
      961 ccgaaaaaca tacgattgta aaacgagaaa ttactattac agacaaagaa aataataaaa
     1021 ctaaaatcaa aggtactaat actttagaag acgataagtt aaaactagat tacgcacttg
     1081 atttcgatca agataaatac acgtatgctg aagcgaaata tacaattaaa ggcgtatctt
     1141 ctaaagaaaa agataataaa tataatgata aatacgaatt tggtaaaaag acagaatatg
     1201 atgaatcaaa aatcaaatta gataaccaag aaaaagtaga tggcacaaaa cgtcaagata
     1261 aaggtaaaat cactgtcgca ttagataaat atagcgacga aaatgaattc acttttgaaa
     1321 ataatataga ttctgacgta aaaaataaca ctcagaaatc tacgttaaat atcggcatca
     1381 aatatgctga agaaccaatt aatttcattt taaaatctag cacaaaattg aaagcagata
     1441 ttgattttga tgatagtggt gcaaaagatt tcaatagtct atcttcaaaa gaccgtgaaa
     1501 aacttgaaaa agaaatcgaa aaaaatggcg gcaaaatgtt tgaatcaatt ttaaaaaagg
     1561 catctaaata actaaaatat aaaaataaga ttggaaggtc atcttgcctt tcaatcttat
     1621 cattggagga gtggttaaat gaaaacaata catttgtttc gcatctacca ctctttttta
     1681 ttgaaaaagt ggtatttgat tatctattta ctatttatat tagcggcact ccttattaca
     1741 ttaacgacaa tccaacatgt aacagaagat gacaatcatt ttaatatagg tgtcgtagat
     1801 aaagatcaat caagtgaaac gaaattaatc ttaaactcta ttggtaaagg gagtaaccta
     1861 ggaaaaaacg tgagcattaa agcatatgat gataagcaag cacatacttt gttaaaaaaa
     1921 cataaacttc aaggctattt tgtttttgat aaaggtatga ccaaggcatt ttataaacaa
     1981 ggcgaactac caatttcagt atatacatat gatcaacaat ccatgaaaag tgtcgtgcta
     2041 tctcagctaa cagattctgt ttaccaacgt cttatgcgat caatgggtgg catcttagct
     2101 tttcaagact tagcaccgaa agcatcacat tctgacagta tcaatgttat gactgatttg
     2161 ctgattacag gattaaaccg ttcaggtgca tttaacttag aaccgattca tttatacgat
     2221 acgggcagtt attatgcaat tacaggattt ttaacaacgg tattcatctt tgcactatct
     2281 ttatttacag ttttgaaaat gaatcaagat actgtattga aagcgcgatt gaaaatgttt
     2341 catttttcta aagagcgttt attaatcatt cgtacgttga ttacatggtt ttatactatg
     2401 ttatggagta tcgttggtgt agtttggatt gtgttcagta ttccgaatat ctttgaatta
     2461 tataattggc caacgttagc cattcattta agctattatg ttaccttttt aatactgtgg
     2521 ttattactca tcgaactttt aacaacaggc ttgttgaata gtattagtaa agtcatatta
     2581 gctattgtca tcctggtgtt atctggttta acaataccta cgattttttt acaacatata
     2641 gcaaatggtg ttttcaatat tcaaccgttc gcagttgtaa caaatcaatt gttagaaatt
     2701 attttaaaca actacatttt agaactgcac cctagtttct atcttagctt catcgcactt
     2761 ttgataatta atttagctgt attggtttgg aggtatcgtc aatgaagcct tatatacaac
     2821 ttgttgtgtt caagcaatgg ttacaataca tcttgctcgt aacaaccatt gtcatcgcac
     2881 tcgtacttat tggtatcggt taccgtgtag cacatgacaa cttcaaaata ccgattacca
     2941 ttcaagattt agaccaaacc actgcatcaa aatcattcgt caataaaatt aaacaatctg
     3001 actatgtaac tattaaaaaa gtcgatgaag atgaaagcta tattgaagat gatgttacta
     3061 aaaaggaagc tattttaagt atgcaaattc ctaaaggttt ctctcaaaaa ttaaaagaga
     3121 accgtttaaa agaaacgata cagttatatg gtagagatga ctttataggt ggtattgctg
     3181 tagaaattgt tagtagttca ttatatgagc agcaaattcc taacattatt tatgaacacc
     3241 ttgaggatat gaaacagcat caatccatcg atgctatcaa caagtcctat cataaacata
     3301 cacctgaatc taaaatcaaa tttgtgtcgc ttactaaaca agcacaacac tctatttcaa
     3361 ttagcttaat ctttgcggtg attttatttg ttagcgctgt tcaagtagta cttcattatc
     3421 gtttaaacca acaagcagca ttgcaacgat tatcacaata tcatttaagc cgtttcaaac
     3481 tatatagtac ttatgtaatg acacatacga ttttgttatt gttggtacta ttggcagtta
     3541 gtctatattt gtctcaacca ctcagcttaa tattttactt aaaatcactg ttacttatat
     3601 tgatttatga gataggtatc gtctttatct tattccatat tcaaacaata agtcatcgat
     3661 tattcatgac atttatatat gcacttgcta tgggtatcgt atacttgatt attttcatgt
     3721 aaaggagcgt aactgatgat agaaattaat aacctttcaa agcgttaccg taacaaacag
     3781 attttcaatc atttaactat gtcctttgat agtaatcgtt taaccgtatt acttggtgat
     3841 aatggtgctg gaaaatcaac attacttcgt atgattgctg gtattgaaaa agctaatgat
     3901 ggaactatca actatttcgg cgaaaaatgg aatcaaagac aaatacaaaa tcacatcggt
     3961 tatgtgccac aagacattgc gttatttgaa cacatgacag tggctgaaaa cattaaattt
     4021 tttaaatcac tttgtaaaaa tccaattaac gatacaacta tcaacgaata tttacagcaa
     4081 ttaaactttg atgatacgtc tgccaaagta tctacattgt ccggtgggaa taaacgtaaa
     4141 attaatatat tagtaggttt actaggtcaa cctcgaattc tcattttaga tgaaccgaca
     4201 gttggtattg atttaaaatc tagacatgac atccaccaac tacttaacat catgaaatct
     4261 aaatgtttaa ttatattaac tacccatcat ttagatgaag ttgaagcact tgcagatgat
     4321 atcaagttaa ttggccaaga tcctttttat caacatgttt tagaggacaa acaatggact
     4381 tatacctatt attaaaacga aaaaatccca agctgcgtat gatatcgcaa cttgggattt
     4441 tctgtattat ctactttgca agtatgacgt tgggtctact gcatattgat taccgatgcc
     4501 accagacata cgttggaagt gtacgtgagg cgctgttgaa ttacccgtac tacctgaata
     4561 tgcaatttgg tcaccagctt tgactttatc accagctgaa acagttaaac gattattatg
     4621 catataccat tggtagttat tactgttcgc ttctttaatc gttacttgat tgccgccacc
     4681 atagttactc caacctgctt gtactactgt accatcagtt aatgagtaaa ctggtgaatt
     4741 ttcaggcatt gcatagtcga caccgtaatg cgcaccacca ccgtgatatt gtccatatgg
     4801 ttgtagttgt ttacgacttg ttaaccagct tgcgtctttc gcatgaccac tagctgtcgc
     4861 tttacttgct gatccaccat tttgattaga tgtgccatta ggataattga cctttccatt
     4921 accatcatgg ctgttatacg cttggttgtt gttactatgt gaataatagc tcgcgtctgg
     4981 acctacattt gattgataac catattgatt aatatgctgt tggctttgac tcgctgtgta
     5041 gtcattgtta tctcctgctg ttgctggatt cacatatgtt tggccgcttc cattggcatt
     5101 tgcatttttt ggataacagt tataaaaata atgcgtatgt ccttgagcat ctacgaatgt
     5161 atagctatat tctttattat caaacattgc ttgattccag ttaccatcag gtgtgtgatg
     5221 ataatcccca ttagaatcaa ttgtataata agtaccataa gatacgtctt gtgattgtgt
     5281 tgacatttgt gtatgtgctt gttgggtgtt tgtcgtttct gctgcatctg cttgatgcgc
     5341 cattgtaaat gtagcgaagc ccatcgttgc aatcgctgct gctgttaatt ttttcatgta
     5401 taaaacatcc tccattaaag ttaaagttag ttttcaatta aactgtactg cacatactaa
     5461 aagaattaga caactgagta aaggatttaa ttctcatttt ccaactattt aatattcccg
     5521 aaatgtttta ctaaactcat tacattgtca ttacaaaata gccatacatt gatattaaaa
     5581 tgacatctct cactgcattc gtttaacctt ttataaattt tcaaaattaa caactaatcg
     5641 ttcgtccatg ttcgcgattc aacgctaatg catgatagta atcatccatc aaatcatatc
     5701 aaccaaattc cattatcaat cgctattgat tgtcattcaa ctttctaata gtgatatgct
     5761 tctcaggctt aaaaatcgtc atatcttttc tattaattaa atcatctgtg agctttaatg
     5821 ctactaattc attgctgcca taatacttaa tatataacgt tcttgtagtt aaatttatta
     5881 cggtctgata catcgtatag tgattgtcag catcatgcgg acgtacaatt ccaatcggta
     5941 tatttaccgc atctaataaa taaaatgcat tcattaaatc catttcttta tcattgtttt
     6001 gagcaatgtt tgctttcata aatgccattc tcacaaagcg ctcagttgaa gtaaatccac
     6061 ctggcaatcc aaatgtacct gcttcattgc ctaaaggttc aatcgttaca ccttccaata
     6121 aatttgctgt tgctggataa ggagaaatat tgatatattg tcttaaatta ctataatgcc
     6181 aatttaagtc tggatgattt gttaagacac caataggatt atcttttata accacttcac
     6241 cctctttaaa tgaaacttcg actgtatgtc cagttgcatc ggaaacatga taatgcaatg
     6301 gcggaacttc accgatgtca tttaaatata cagctacaac atgtatttgg gatgcttgtt
     6361 gtttcatatc ttcaatgctt gttgtatatc ccaaaatcca tgtcacaatt tcattttgcg
     6421 taatattcat cgcgtccgct ttgtgtgttg atccatatga actataacct cggaaatatt
     6481 gtgttgaaat ggcaacgcca tgttcattaa caccatcacc ataaataaaa ccttccatat
     6541 ctgttcctgt gccaataaag ccatattgcg tttggcctgt cgtgccagtg cgagatttcc
     6601 aacgataatt tctaggcgtc actgctggcg aaccatctaa tggataatca taatccatcg
     6661 tgcgtccaag aagtacttga ttatttaaag tttgtattgt gaatcctgtg cacattgttc
     6721 tcactcctct gtaccttcat ttactttaat cactttcaaa taaagctgtt tcacttaaac
     6781 atactataaa aaatcaatta tacaagcaat taattgatat tcattctcaa taactgtggt
     6841 atgatatgta aggaaatcat gacttatgtg tgagtgaacg atcatctata catccgttca
     6901 cttcatctca tgactttcta tatttaattt ttacaaggag tgacatctgt gaataacaca
     6961 caatcttcac cacgcagtaa tattattatt gcgattatgt tgtctgcatt aacatattgg
     7021 ttgtttgcac aatcatttat taatatagga cctctcgttg gtcaaacgta tcaaacctct
     7081 cctgccgtgt taaatttatc tattagttta acttccttcg ccacaggtat cttcatggtg
     7141 gctgcaggtg atattgctga taaaatagga caactgagaa tgacatacat gggtctcata
     7201 atcagtatgt ttgcatctct tctattaatt atatcggaca tcactgcact gctcatcatc
     7261 ggtagaattt tacaaggtct atcagcagct atcttgttac cttcaacagt tggcgtgtta
     7321 aataatcaat ttaaaggaga acatttaaga cgagcgatta gttatctaat gattagtact
     7381 gttggtggca tcggcctagc tggtgttatc ggcggtttaa ttgcctcaaa tttcggatgg
     7441 caaacgaatt tcatcattag tatagtcatt gctttcattg ccatattgct tctaaaaggc
     7501 acacctgaaa aagtaagtca acatagccac cgtcatccat tcgattacaa aggtatgtcg
     7561 attttcgctg ttatgattgg tagctttaca ttattgttaa cacaaggatt cgaacaaggt
     7621 tggtttagta cattttcaat catttgtctg agcattttta tcatcactac gttgatattc
     7681 atcatcatcg aacgtcgaca tgaagtacct tttattgatt tctcagtatt acgcaaccgt
     7741 ccgttcattg gtgcattttt aaataacttt gttttaaata gcggtctagg cgtaacagtg
     7801 gtctttttca tatatgctca aacacacctt ggtttatcag ctgcgcaatc tggacttgtt
     7861 acattgccat atgccattgt ggcagttgcg atgattcgtt taggtgaaaa agcaacatta
     7921 cgtttcggtg gcaaattgat gctcatcatt ggtccgttgt tccctgtcat cggcatcact
     7981 attattagca tgactcaatt attggcatca caatatgtca ttgcagttat cattggtttc
     8041 gtcatatgtg cgataggtaa tggtttagtc gcaacacctg gacttacgat tgcaattttc
     8101 agtatgccta atgaaaaagt tggtttagct acaggattat ataaaatgag tggtacatta
     8161 ggtggcgcct ttggtatagc actaagtact acagttttca gtatgttaca actaaactat
     8221 gcaccaagtg tagctgcaac cgtaacattt atagtcagca ttgtattgat gatccttggc
     8281 tcattgtctg catacatgat cattccaaaa acagttaaat cttaaatata atagaagaat
     8341 tatgtttcga aatatcttta tcactttaaa atgatataca agaaatccaa gaaaaataag
//
LOCUS       DALAGF010000117         8399 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.117, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000117 DALAGF010000000
VERSION     DALAGF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.117"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            199..3153
                     /gene="fdhF"
                     /locus_tag="O2692_002373"
     CDS             199..3153
                     /gene="fdhF"
                     /locus_tag="O2692_002373"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCZ4299979.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTAIKEKHGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRQPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            3153..3626
                     /locus_tag="O2692_002374"
     CDS             3153..3626
                     /locus_tag="O2692_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HCZ4299980.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            3854..4087
                     /locus_tag="O2692_002375"
     CDS             3854..4087
                     /locus_tag="O2692_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299981.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            4109..4885
                     /locus_tag="O2692_002376"
     CDS             4109..4885
                     /locus_tag="O2692_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCZ4299982.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            5196..6320
                     /locus_tag="O2692_002377"
     CDS             5196..6320
                     /locus_tag="O2692_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HCZ4299983.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLFVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(6412..7365)
                     /locus_tag="O2692_002378"
     CDS             complement(6412..7365)
                     /locus_tag="O2692_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HCZ4299984.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(7727..8227)
                     /locus_tag="O2692_002379"
     CDS             complement(7727..8227)
                     /locus_tag="O2692_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCZ4299985.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
BASE COUNT         2948 a         1204 c         1607 g         2640 t
ORIGIN      
        1 taatttcgcg caaaatattt caaataaaaa tgattgtaaa aaggcaaaat acaaaatttt
       61 acttaacaac tagtacataa agtaatacaa ttaaattaat tctatctgaa agatgtgtgg
      121 ggcatcgtta ttttaggtgg atatgagcaa tttattaaaa gtcatttacg gaaaatatat
      181 atagacgggg tgagtaatat gcaagaacat ttggtggtta cacttgatgg aaaagattat
      241 cttgtagaac caggtacgaa tttacttgaa tttattaaat cacaagatac ttttgtacct
      301 tcaatttgtt ataacgagtc gatgggacca attcaaacat gtgatacatg tactgttgag
      361 attgacggta aaattgaacg ttcatgtagt acggtgattg atcgtccaat gactgtaaat
      421 actgtgaaca atgatgtgaa agatgctcaa aaagaagcgc ttgatcgaat tttagaaaag
      481 catatgctgt attgtacagt atgtgattat aataatggtg attgtgaaat tcataatacg
      541 atggatgcat gggggcttca gcatcaaacg tatgaatata aagagaagcc atacgaaaaa
      601 gattacggcc cattttatcg ttatgatcca aatcaatgta tcttatgtgg gcgttgtgta
      661 gaagcatgtc aagatatcga agtgaatgaa actattagaa ttgattggga tcgtgaacat
      721 ccacgtgtta tttgggataa tgatgtaccg attaatgagt cttcatgtgt atcttgtggt
      781 caatgtgcga cggtatgtcc atgtaatgcc atgatggaag tgaatatgga aggtaatgcg
      841 ggttatatga ctgatacgga acctggttca ttagcagcaa tgattgattt aactaaaaaa
      901 gcagaacctg gttatggtcc actatttgca atctcagatt cagaagcaga aatgcgtaaa
      961 gagcgtatta agaaaactaa aacagtgtgt acttattgtg gtgtgggttg ttcatttgaa
     1021 gtttggacta aagatagaga aattttaaaa gtacaaccat cacatgattc tccagcaaat
     1081 aaaattgcga cttgtgttaa aggtaagttt tcatggggac atattaattc agatcaacga
     1141 ttaactaaac cactagtaag gaaaaatggt gagttccatg aagtagaatg ggatgaagct
     1201 ttaaacgtca ttgcagataa ttttacagct attaaagaaa agcatggccc agatgcacta
     1261 tcattcattt cttcttctaa agcgacgaat gaagaatcgt atttaatgca aaaattagca
     1321 agacaagtta ttggcacaaa taacgttgat aactgttcaa gatattgcca agcacctgca
     1381 acaaaaggct tatttagaac ggttggacac ggcggtgact caggtagtat tgaagattta
     1441 gaaaaagcgg caatgtctgt attgataggt actaatacag ccgaagctca tccagttatc
     1501 gcatcacgca tgaaacgtgc acaaaaatta tttggtcaaa aaatacatgt atttgatatt
     1561 agaaaacatg aaatggcaga acgtgcggat cgtttttatc aacctaaacc aggtacggat
     1621 ttagcgtggt taagtgcagt gactaagtat attattgatc atgatttaca cgataaagca
     1681 tttattgatg agtgggtaga tgattttgat gaatattaca aatcattaga aacatttaca
     1741 atggcttttg ctgaagaagc aacaggtatt cctgaatcag aattgattaa atttgctgaa
     1801 gaatgtgcta aagctgaatc tgttgtaatt tgttgggcaa tgggtattac acaacaagac
     1861 attggtagtg actcaagtac agcgatttca aacttattat tagtaacagg taattatcgt
     1921 cgtcctggta ctggtgcgta tccattacgt ggacataata atgttcaagg atgtagtgat
     1981 atgggaagta tgcctgataa gattactggt tatcaaagta ttgaagcgga tgatattcgc
     2041 gctaaatttg aaaaagaata tggcgttaaa ttgaatccaa aagctggtaa agataatcat
     2101 gaaatggtag aaggtataca tgacggagaa gtacactcat tgtacttata tggtgaagat
     2161 acgggtattg tggattcaaa tattaatttt gtacaagctg cgtttgaaaa attagatttc
     2221 atggtagtcc aagatgaatt tttaacattc acagcaacat acgcagatgt tgtattgcca
     2281 gcaagtcctt cacttgaaaa agacggtact tttacaaata ccgaacgtcg tattcaacgt
     2341 ttataccaag cattagaacc tcttggtgat tcaaaacctg actggaaaat tttccaagca
     2401 attgctaata gattaggatt tgattggaat tacaagcatc ctagtgaaat tatggatgaa
     2461 gtagcacgct taacacctct atatgctggg gtaagttatg atcgtttaga aggattcaat
     2521 agtttacaat ggccagtaca acctgatggt actgatgagc ctattctata cttagaagga
     2581 ttcaattttg ataatggtaa agcaaaatta ttcccattat catttgataa ttactttaag
     2641 caagatgaaa tttatgatat tcatgtaaac aatggtcgat tgttagagca cttccatgaa
     2701 ggtaatatga cttatcaaac accaatgatt aaatacaaag tgccacgtgc atttgttgaa
     2761 atttctccag agcttgctga agatagaggc attcatgaag gtgcagaagt taagctgatt
     2821 tctgaaacag gagaagcggt attacaagtt cacgtgacag atcgtgttaa aggtaaagaa
     2881 atctatattc cattaaataa tgatgcgatg gaaaatggag atctaggtgc aattaactta
     2941 ttaactaata gtgatgttga tcaatacacg gatacaccat cttataaacg tactagctgt
     3001 cgtttggagg taattacgaa acgtggtaag tcaccgttga accctaataa tttccgtgtc
     3061 aataaaaaac gtcaaccgca gtacagtgtt caagtacaga aaaaatggga acgttcagat
     3121 tatgttttcc cgggaaatca ggtggataaa taatggctga gagaatatct tctaaaatta
     3181 gacgcttaga aaaatcagaa gaacaaatta agttagaaag tttaaatgaa gttacagagg
     3241 caattgcagc gaataaagat agtatattaa aagcaatcaa gcttattaaa acattagatg
     3301 atgcaaaact attagatgct ttaaatggtg caattcgagg acgtcaagtc atcataaata
     3361 aatttgcagt tgaacttaat aaagatattt atacagggct attatctaat atggcttcaa
     3421 tggtattttt attaggtgaa ctcaatgtat cagatttaag cgacttccta aataaagtta
     3481 ataaaggatt acacgttgcc aatcaagcga gtccaaatgc taagacaact ataagaagtc
     3541 tattgggcgt attgaaagat gacgacatga accgtagttt aacatacatg cttaatatgc
     3601 taaaaggtat gtctcgagaa gaataacatt acaatgaaaa agtgtttcta catgcaagca
     3661 gtgtaggagc acttttttta atttttgtaa aaattaaaaa gggtgttttt gatggaataa
     3721 atgaataata ttgaaaaaca aaaaaatcac gtgctattgt gaatcaatca tcgacaggcc
     3781 tatgaaacat catgtattat tatgattatg taagttattt aaataaaaag ggtgagtgca
     3841 tattaattcg agaatgttaa aaacgtcagc tgtaatttct aatacattat tagtcatagg
     3901 tttagtctgt ctatttatga tgaaattagt gttagctatt actttttttg ctgtttcatt
     3961 atcaattagt ctagttgtct ttaatatgat gttcagagat agaacaacga tgaaagtaat
     4021 cgttaacgct tcttttttaa tcgtaatatt agcgattgta gtggcatatt ttttattgtc
     4081 taagtaagaa aaatgaggtt ttgtctatat gaagaagaat ttcaagttac gcatttcaac
     4141 gctactattg atagttattt tagttgtttt tgctgtatta ctcatcgtga atgaaactaa
     4201 attgtttaaa aatgatgtga attactcttt tgatgaggct gtttcaatgc aacaagggaa
     4261 aggtattgta cagacaaaag aagaggatgg taaatttgtt gaagcaaata ataatgaaat
     4321 tgctaaagca atgactattt cacataaaga caatgatatg aagtatatgg atatcacaga
     4381 aaaagtgcca atgtcggaat ctgaagttaa ccaattgcta aaaggtaagg ggattttaga
     4441 aaatcgaggg aaagtttttc tagaagctca agaaaaatat gaggttaatg tcatttatct
     4501 tgttagccat gcattagtag aaacaggtaa cggcaaatca gaattagcaa aaggcattaa
     4561 agatgggaaa aaacgctatt acaacttttt tggtatagga gcattcgata gtagtgctgt
     4621 tcgtagtggg aaaagttatg ctgaaaagga acaatggaca tcaccagata aggcgattat
     4681 tggtggtgca aagttcattc gtaatgaata ttttgaaaac aatcaactga atttatatca
     4741 aatgcgatgg aatccagaaa atcctgcgca acatcaatat gcgagtgaca ttcgctgggc
     4801 agataaaatt gccaaattaa tggataaatc ctataagcag tttggtataa agaaagatga
     4861 tattagacaa acatattata aataagacat cggtgcttaa aggagctgga acaatttatt
     4921 gtttcgagct cctttagcgc attctgagtg tgttagttaa atggatttta acctaacaaa
     4981 aaacgctata tagcatcaaa tatgctatat cccacatcat tgttacaaat gtacatgatg
     5041 taaatgaata ttgctgtcta aatgtgcatg taatatacaa tggtgcagat aatacactta
     5101 agtccttaaa aatgaaacgt tagttccaag agtcattttt aaacaatagt gcatgtgata
     5161 aaatagaaaa gaatgaaaaa tatagaggtg acaatatgaa gatagcaatt ataggtgcag
     5221 gcatcggtgg attaacagct gctgcattat tacaagaaca aggtcatact attaaagtct
     5281 ttgaaaaaaa tgagtcagtt aaagaaattg gcgctgggat tggtatcgga gataatgtgc
     5341 ttaaaaaact aggtaatcat gacttagcta aaggtattaa aaatgctggg caaatcttat
     5401 ctacaatgac agtgttagat gacaaagatc gcctgttaac tactgttaaa ttaaaaagta
     5461 atacattgaa tgtgacgtta ccacgccaaa cattaattga cattattaaa tcttatgtaa
     5521 aagatgacgc aatatttaca aatcatgaag tcacgcatat agataatgag acagataaag
     5581 ttaccataca tttcgcggaa caagaaagtg aagcatttga tttatgtatt ggtgctgatg
     5641 gaattcattc taaagtgaga caatctgtaa atgctgacag taaagtatta tatcaagggt
     5701 atacatgctt tagaggttta attgatgata ttgatttaaa gcatccagat tgtgcaaaag
     5761 aatactgggg aagaaaagga agagtaggta ttgttccgtt attaaataat caagcatatt
     5821 ggttcattac aattaactcg aaggaaaaca atcataaata tagttcgttt ggtaaacctc
     5881 atttgcaagc atactttaat cactatccaa atgaagttag agagatctta gacaaacaaa
     5941 gtgaaacagg tatcttattg cataatattt atgatttgaa accactcaaa tcttttgttt
     6001 atggtcgtac tattttacta ggagatgcag cacatgcgac aacgcctaat atggggcaag
     6061 gtgctggaca agcaatggaa gatgctatcg tattagtaaa ttgttttaac gcatacgact
     6121 ttgaaaaagc attacagcgt tatgataaaa tacgtgtcaa acatactgca aaagtaatta
     6181 agcgttctag aaaaatcggt aaaattgccc aatatcgtag tcgtttattt gttgcagtta
     6241 gaaatcgtat tatgaaaatg atgccaaatg cattagcagc tggacaaact aaattcttat
     6301 ataaatcgaa agaaaaataa tacaacaata tgaaaacccc cgtatgttga aacgagagct
     6361 caacatatgg gggttcttgt ttttataatg ttattataat aaattcaatt attagttaac
     6421 gacaaattgt ggtttctcac cttgaacggc actaattgca gcattagcaa caattttaga
     6481 catcatgtca cgtgcttcaa atgtagcatt accaatatgc ggtgttaata ctacattatt
     6541 aagtgatttt aagtcatcgg taatatctgg ttcaaattca tatacatcaa gtgcagcacc
     6601 ttcaatttca ttatctttca atgcttgcac tagtgcttgt tcgtgcacga ttggaccacg
     6661 agaggcattg attaaatacg ccgtagattt catcatttta aattgttctg tatcaattaa
     6721 atgatgcatt ttaggattat aagcagcgtt gatagtgata aaatctgcat tctttaatag
     6781 tgtatctaaa tctacatatt ttgcaccgat ttctcgttct ttttcttctt tgcgattagg
     6841 tccagtgtat agcacatcca tgtcaaatgc tcttgcacga cgagctactg cactaccaat
     6901 ttcacctaaa ccgataatgc cgattgtttt cccagatact tctctacctc tgaaaaataa
     6961 aggtgcccat ccatcaaatc cagttgtacg tgataattgg tccccttcaa caatacgacg
     7021 cgctactgca agtactaatc caattgttaa atcagcagtc gcgtttgttg atgctttagg
     7081 tgtgtttgta acatctatac ttttttctcg ggcatactcg atatcaatat tattaaaacc
     7141 agcgccatag ttggcaatga tttttaagtc tttaccagca tcgataacat ctttatcaac
     7201 gtttgtagat aataaactaa ttaaggcagt cgcgttttta acacctttaa ttaaagtgtc
     7261 tttatcgact aatcctttac cttcatacat ttcaacttca aaatgttctt gtaaaagttt
     7321 taaacctact tctggtattg caccagcaac ataaactttt tccataaaag atcactcctt
     7381 ttatcttagt atagtagaag attagacagt atacaactat gtcatgatgt cttgtgtatc
     7441 aatgatgtaa gcgcgtactt ttgatggagg tgatataact taggcactgt agaactatga
     7501 atattgtaat gtggaaaaac tggatcaatt aaattagata acgtagtttt aaagttaata
     7561 gtattagaaa aaattaatat tttgaatatg ggaggaaata taaataagta ggtggcaacg
     7621 aaaaatagca aaaaaagagc ttctcctata aaggaaagct caaagttttt tgatgacata
     7681 tgtactagaa ttaagtttca agacaatatg tatcatcgtg tttatattaa atatggatgt
     7741 agttgtagtt acctgcttca cttgcagaaa tagttctaga acttactgag aaaggtccgc
     7801 cactataatt catttctgaa attgtaactg aaccatcact gtttacactt tctacatatg
     7861 caacgtgacc aaatggtcct tcagaagatt gtaggatagc gcctttagaa ggtgtatgat
     7921 ttactgtgaa tccagcacct tgtgcagcag cagcccaatt attagcattt ccccaagtag
     7981 aaccgatttc tccgccaact ttatcatata cataccaagt acattgtcct gcagtgtata
     8041 agttaccaga atgtgaaatt gatgatgtag ttgtcgtagt tgtcgtagtc gttgtagttt
     8101 gagtcgtgtt gtagttatag ttgttgtaat ttgtataatt ttcagcagca tctgcatgat
     8161 gtgcttgacc tactaatgct gtgccgattc ctgctgttaa cgtagttgct gttactaatt
     8221 ttttcatgaa taaagtcctc caaagttcta tatctttttt tataaataaa acgtagcgac
     8281 tgttttattc tcacatctcg aattgatgac aatagttact ttaacaaaat taatgcttct
     8341 tgtggggaat gttattgatt tgtaaaagaa taaaaaaact ttgactaatt ttgtaataa
//
LOCUS       DALAGF010000118         8273 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.118, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000118 DALAGF010000000
VERSION     DALAGF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.118"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..173)
                     /locus_tag="O2692_002380"
     CDS             complement(<1..173)
                     /locus_tag="O2692_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCZ4299986.1"
                     /translation="MNYFRYKQFNKDVITVAVGCYLRYALSYRDISEILRERGVNVHH
                     STVYRWVQEYAPI"
     gene            435..620
                     /locus_tag="O2692_002381"
     CDS             435..620
                     /locus_tag="O2692_002381"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299987.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
     gene            complement(843..1421)
                     /locus_tag="O2692_002382"
     CDS             complement(843..1421)
                     /locus_tag="O2692_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ4299988.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(1685..2065)
                     /gene="blaI"
                     /locus_tag="O2692_002383"
     CDS             complement(1685..2065)
                     /gene="blaI"
                     /locus_tag="O2692_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCZ4299989.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(2055..3812)
                     /gene="blaR1"
                     /locus_tag="O2692_002384"
     CDS             complement(2055..3812)
                     /gene="blaR1"
                     /locus_tag="O2692_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCZ4299990.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            3919..4764
                     /gene="blaZ"
                     /locus_tag="O2692_002385"
     CDS             3919..4764
                     /gene="blaZ"
                     /locus_tag="O2692_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCZ4299991.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            complement(5290..5709)
                     /locus_tag="O2692_002386"
     CDS             complement(5290..5709)
                     /locus_tag="O2692_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ4299992.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            5833..6804
                     /locus_tag="O2692_002387"
     CDS             5833..6804
                     /locus_tag="O2692_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCZ4299993.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            complement(6976..7068)
                     /locus_tag="O2692_002388"
     CDS             complement(6976..7068)
                     /locus_tag="O2692_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCZ4299994.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(7225..7572)
                     /locus_tag="O2692_002389"
     CDS             complement(7225..7572)
                     /locus_tag="O2692_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4299995.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNHEGF"
     gene            complement(7591..8208)
                     /gene="cadD"
                     /locus_tag="O2692_002390"
     CDS             complement(7591..8208)
                     /gene="cadD"
                     /locus_tag="O2692_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HCZ4299996.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
BASE COUNT         2943 a         1206 c         1087 g         3037 t
ORIGIN      
        1 aaaattgggg catattcttg aacccaacgg tagaccgttg aatgatgaac gtttacacca
       61 cgttccctta atatttcaga tatatcacga taactcaatg catatcttag atagcagcca
      121 acagctacag tgataacatc cttgttaaat tgtttatatc tgaaatagtt catacagaag
      181 actccttttt gttaaaatta tactataaat tcaactttgc aacagaacct catttactat
      241 atgggcatct ctttcattat taatacaaag ctttgtatct ccgatattag gcaaaggtgt
      301 aaacgaatta ggtgatacta taggattcaa cattatatta atcgtttctt taataatgat
      361 tatcttctta ttttttataa atcgtagtaa aaatatctcc tatgattata taaaagttca
      421 aagaggtgaa gccaatgaat aaattagaaa aatggtcttt ggtatttatt atttttacat
      481 ttctctcttc attagcactt ttatatttca aatataattt tagttcggtt atgccagtcc
      541 taagtctttc tattttaact atactaatat tgttgacgag tattattact gtagtttcag
      601 ttaatgatga agatacttaa aacgttcatt agtgaaacag gtattatatt aaaaaagcgt
      661 acgaatttgt acgctttttt cttgtgttat tattaaaatc tcataacagg aaaatattat
      721 gagattttag acgctgtcag aacgtctctt ttacatcgag tgacattgaa tcaaagtctt
      781 tgtatgttaa tgatatataa aattatttag tagattcatt atagtaagct agattagttg
      841 ctctacttgt ttttattctc ttttgcctct aatttattga gataacgata aatagttgta
      901 cgtgaaactt gccactgctc tgcaattgtt ttaattggcg taccattatc ataaagtgtc
      961 ttaagtaagt ttaaatcttg tttatttaat ttttcaggtc tacctccata tcggcctcta
     1021 gcacgtgctg caattcgacc ggctgatgat cgctctaaaa ttaaattacg ttcaaactct
     1081 gcaaatgctg cgaataaatg aaataataat tgtcctgtag aacttgattt atccatcgtt
     1141 atattttctt ctaaactatg aaaactcaca cctcgttcgt taagttcatt aactaacgtg
     1201 attaaatcct ccatgttccg tcctagtcta tctaatctcc aaacaacaat cgtatctcct
     1261 gacctcgcaa attcaattgc tttatctaaa ccaggtcgct tactttttga accactcata
     1321 tggtcattaa atatcttttc acaaccatat gtatttaatc gatcctcctg taaattcaaa
     1381 ttttgtaatc cagttgaaac tcttgcataa cctattttca aaatacagac ctcctatata
     1441 cacctttagt gtaccataac ttgattacga ggtcttttgt gaacatagat tttttgttgg
     1501 gtttttgaac acataattct atgtaatact tacttattta gacaaaaaac aagagtgtac
     1561 agaaaatggt tattttctgg acactctcat ctttaataat atattttaaa tgtcattaac
     1621 aaaactaatt taataagagt caagtatggt ttacatatgt attatgtttg aaacatacct
     1681 ctaattactt tttactaata tcatttaaaa tgtctcgcaa ttcttcaatt tctttgttat
     1741 ttaattcttc atttttcgca aaattcagca ctaaactttt catatcccct ccatacagtt
     1801 tattaagaaa ggttttagca gttttcattt taatatcgtc ttctttaata tttgatgagt
     1861 aaaaataaat attttctgat ttgtatcgtt ttataatctc ttttttatat agtcttgtga
     1921 ttaatgttct aatcgtttta tcactaactt ctttatattt ttgaatttca actacaattt
     1981 cattagctga tactgatttt ttatcccata ttatattcat aacatcccat tcagccatag
     2041 atatttcaac ttgcttattg gtcatttaaa acacccattc cttttaatat tttttcactt
     2101 attaattcag catttttccc agacgggttt ccatctgata aatgtgtagc aaaataatac
     2161 ttatcatgat ttgtaattac gtaacctaca aaccacccat tattatactt cccgtttact
     2221 atacctgtac ctgttttccc atacagttca tacttttcat ttttcttaat caataatgaa
     2281 gaagataatt gattctttgc ttttttacta aaatggttat tttgttccat catattttta
     2341 aaaactatta cttgttcaag attagatatt ttcaaactat cttccatcca atagctttta
     2401 taacttccca aattttcatt accataattt aattgcttga gttgagccgc agtatagttc
     2461 tttggtattt gattactaat acgttcgaag taccaattaa ctgaattttg cattgctgta
     2521 tttaaatctt gttccttatt ccaagactcg aaaagataat gtttatgatt ccatgacata
     2581 cgtgaatttt tatcacttat aatatgacgg tcaagtccaa acagtgctaa ataaattttg
     2641 taagttgaat caggagaata tctttttcta ctttcttttt cattataaat atagtatttg
     2701 tctttcttca tgctgtacat aacgaaagat ccactatttg aaccaaaatt tttactttca
     2761 tctaaaattt ggtaatcgct ttgtaagggc tttttataat tataatcagt taaggattgc
     2821 cccattaaaa actggctttg aattatcatg ataaaaaaag taaaaataca aataaaaatt
     2881 agaattaatt ttgactgctt tttcagatcg gcttctttta tattaattaa tctacttttg
     2941 agtaatgatt ttttaccatt aaatgaatgg cttaatatat ttttgttgga aaatggggtt
     3001 tttaatacgg agtccataat agattccgca taagttttaa attcattttt gttaatatta
     3061 ttaaggacaa atctatcggc ttctacttca ttgtcatgaa ttatctttct ttttacaata
     3121 tgtactaatg gattgtaact cataataata gagaagatat taaagataat taaatgtaaa
     3181 gtatctctat ttttagcatg agcatattca tgcagtatta tatattttag tcttttatca
     3241 attacacttt taaaatatga actaggaatc aaaataatat atttcccata ccaaaaagtt
     3301 attggagatt gaatagcctc tgcttttcga atcacaatat tttttttata ttgatggttg
     3361 aaaagtatcg tatctatttt atttttttca ttttcgttta gataaagtga ctgtttcttt
     3421 aaatatttaa gatataataa ggctttcaaa aatttaaaac ttaaaataat aactaaaact
     3481 atccaaataa ctgtgcagat attatcaatt gaatcccaat taaacttatg gatatctgtt
     3541 gtgaactctt gaataggttt ggtggtattt atgttatggt tcaaatcgtg tgacttactt
     3601 tcaactgtgg gcgcttgatt attcacatta ttaaatttta taaaagagaa tttaattggg
     3661 ataaaaggaa ttaatccagc aagcatagtt agataccaaa ctttataatt taaactataa
     3721 ttaaaatagc gttttaatat gtacctaaaa aatactaaca atagaaatat aaaacaaaag
     3781 cttactacgc tcattattag taatttagcc attttgacac cttctttcaa atatttataa
     3841 taaacaattg acatcaatat tacaattgta atattattga tttataaaaa ttacaactgt
     3901 aatatcggag ggtttatttt gaaaaagtta atatttttaa ttgtaattgc tttagtttta
     3961 agtgcatgta attcaaacag ttcacatgcc aaagagttaa atgatttaga aaaaaaatat
     4021 aatgctcata ttggtgttta tgctttaaat actaaaagtg gtaaggaagt aaaatttaat
     4081 tcagataaga gatttgccta tgcttcgact tcaaaagcga taaatagtgc tattttgtta
     4141 gaacaagtac cttataataa gttaaataaa aaagtacata ttaacaaaga tgatatagtt
     4201 gcttattctc ctattttaga aaaatatgta ggaaaagata tcactttaaa agaacttatt
     4261 gaggcttcaa tgacatatag tgataataca gcaaacaata aaattataaa agaaatcggt
     4321 ggaatcaaaa aagttaaaca acgtctaaaa gaactaggag ataaagtaac aaatccagtt
     4381 agatatgaga tagaattaaa ttactattca ccaaagagca aaaaagatac ttcaacgcct
     4441 gctgctttcg gcaagacttt aaataaactt atcgcaaatg gaaaattaag caaaaaaaat
     4501 aaaaatttct tacttgattt aatgtttaat aataaaaacg gagacacttt aattaaagat
     4561 ggtgttccaa aagactataa ggttgctgat aaaagtggtc aagcaataac atatgcttct
     4621 agaaatgatg ttgcttttgt ttatcctaag ggccaatctg aacctattgt tttagtcatt
     4681 tttacgaata aagacaataa aagtgataag ccaaatgata agttgataag tgaaaccgcc
     4741 aagagtgtaa tgaaggaatt ttaatattct aaatgcataa taaatactga taacatctta
     4801 tagtttgtat tatattttgt attatcgttg acatgtataa ttttgatatc aaaaactgat
     4861 tttcccttta ttattttcga gatttatttt cttaattctc tttaacaaac tagaaatatt
     4921 gtatatacaa aaaatcataa ataatagatg aatagtttaa ttataggtgt tcatcaatcg
     4981 aaaaagcaac gtatcttatt taaagtgcgt tgcttttttc tcatttataa ggttaaataa
     5041 ttctcatata tcaagcaaag tgacaatagc attttacaag tattttttat tgttcgatta
     5101 gggtaaacct aaataataca taaattatat tacaatatgt ttattataga ggctaaagta
     5161 tcttagaaat ttggatagta ggtttttcgg gaataaaagt atagttggct ttaatatata
     5221 attaagctaa gctatcattt atatttactc gattataatt cgcacttcaa cttttttata
     5281 acgtaaaaat taaaacgtag ttttatttat ctttagtaat atctttttaa acactacagt
     5341 ttcttcatta gttaaattag cgaagtaatg attattaagt tgataggctt tcttttctaa
     5401 tgttggccat aagtcttttg ctttatttgt actgtttatt agcacagttc ttctattttc
     5461 attatcagtt tgacgtgtta ttagatcctt tttggacatc ttatctaata ttccagatat
     5521 tgttgaaggt tctaaattta aagtattacc aatcgcttta ggtgtattca aattactatt
     5581 aatcaagcaa ttcaaaacac catattctgc tggagtaata ttatagggac ttaataaact
     5641 tttaaaatgt ttaaaaactc tattctgaga agtactcaac aaataattta tacaattatc
     5701 taattccaca actaaaacct ctcttaaatc attagtattg actcgcttat catatcatga
     5761 taagttttgt aggaaaatag ttttgcaaca aaaacatact tctacaaaac aattgtgaaa
     5821 gaaggctaat aaatgttcaa tgcggtagta gtgagaaaaa gtgcatcaaa tgttagttat
     5881 aaagtagaaa aagttgatga atctcattta tcgaagggtg atgtattagt aaaagtggta
     5941 tactcttcaa taaattataa ggatatgcta gctttacaat ataaaggcgg tgtaattaga
     6001 gattatccta tgattcctgg tatagatttt gcaggtattg ttgaatcaag ttcgaatgat
     6061 aagtttaaag aaggcgataa tgtattagtt actggttatg atgttggcgt gacacataca
     6121 ggtggatttt cagaatatgc acaagtgcct ggagagtgga ttgttcctat tccccaagaa
     6181 atgtctatta aagagtctat ggtatatggc acagccggtt ttacggcagc attatcaatt
     6241 tttgaactag aaaaatatgg aatgaatatc aaatcacaac ctgaaatttt agtgtctggg
     6301 gctacaggtg gtgtgggcag tgttgctata caaattttac ataaaattgg atatgaaaat
     6361 atcactgcta tcgtaagaaa agaaaagcaa atagaaatcg caaaaaaatt aggtgcaaaa
     6421 caagtaataa taattgaaga tgataataaa ccgcttaaaa aaggtatttt tgattatttc
     6481 ttagatacag tggggagtaa tgtaacatta tacggtttaa aacgtctaaa ttatcaaggt
     6541 gttgctactg tatgtggaaa tgtagcagga aattcactta acgctaatat attaccgttt
     6601 attcttagag gtattaaatt aattggcatt gattctgtat atgttgatca taatatccgt
     6661 gaagatatat ggagtaagtt agcaaatgaa tggaatatag gaaattcact tctatacaat
     6721 gaaattggat ttgaagaatt ctataaaacg atagatcagc ttttaaaagg acaacattta
     6781 ggaagaacta ttttaaaagt ataagtgtga gtattcatta atacatttac tattaattca
     6841 atcattttgc atttctctac taaccagtgt tataatttat ataacgaaaa tgattaacaa
     6901 ttaatgcacc aatccccaca ctattgcagt agtgtgggga ttttttcgat gctggcttat
     6961 aaagtcgcct attaattatt tgttgcgttt actaagccaa taagtataat acgcaacagc
     7021 acagccactg atgactggtg ctatgatgtg aacgaaaatg attagcaaag taatgcacct
     7081 cctctcttcg tcaattgacg cctgagagat aggcgactct attattatac catctgtgaa
     7141 tatacaagca tatctacttc cgataagtcg tcttatgtta tataactttg ttttttatag
     7201 tatcaagaca agaagaaact cgtttcaaaa accctcatga tttttagaga aaaactctct
     7261 tatttcatcg tctttaataa aataatatgc catctttcca tctttataaa aatcaagtac
     7321 ctcattttta tataaaagcc ttaaatgatg tgaagttgaa gcaacactca ttttcaatat
     7381 caaagaaata tcacaaacac acaactcatc ttctttaatc aaagataata tgattttcaa
     7441 tttcttctca tcacaaattt tttctaaaat gttaagtaat ttcttagatt tatcatcttc
     7501 taaaaaactt aaagcattgt taactttatc ctcatgtaca cagatcacat cacaagtatt
     7561 ttcataactc atataaaatc acaccctaaa ttaaaatata aatcctaaaa ttgtttgaat
     7621 agtgtcattt tcaataataa taaataaacc taaagctata taaataatag ccataatcca
     7681 acgactaaat ttctcaacaa tttctccaat tcctggaata ttagctaatt tttgtgcagt
     7741 aaacaccaag aagaaaatta aaattaaaaa gacaaacaga gtaagtaata aattagtaac
     7801 acttaatgtc acaaaatacg gaacaaataa accaatatta tcggcgccac aacttgctat
     7861 cgtaacaatt gcaaccgtac caactaattt agacaatcct ttttcattca attctttttt
     7921 agctctcttt tctccttcac aatcatcata aatagccact ttaattccaa gataaattgg
     7981 tattaaaccc aataaaccta atatccactt ttctggaaca taatttaata caaaagctaa
     8041 aaacaaacta actaatatta aaataataga ccctaaatat tgaccaacat aaatatctct
     8101 atattctttt ctagtctttg ctctagcaaa aaatattaat aaaattacta ataaatcaac
     8161 tgctgtagca atataaagaa cagcagcagc aacaaccgtt tgaatcataa tacacctcat
     8221 tcaaaaatat ttttgaatgt attataactt atatttttaa ataatcatag gtg
//
LOCUS       DALAGF010000119         8200 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.119, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000119 DALAGF010000000
VERSION     DALAGF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.119"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            203..1690
                     /locus_tag="O2692_002391"
     CDS             203..1690
                     /locus_tag="O2692_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCZ4299997.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGNPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            2336..3295
                     /locus_tag="O2692_002392"
     CDS             2336..3295
                     /locus_tag="O2692_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCZ4299998.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(3357..3560)
                     /locus_tag="O2692_002393"
     CDS             complement(3357..3560)
                     /locus_tag="O2692_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4299999.1"
                     /translation="MDKESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKEIDENHLNDAT"
     gene            3738..4250
                     /locus_tag="O2692_002394"
     CDS             3738..4250
                     /locus_tag="O2692_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HCZ4300000.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            4592..5332
                     /locus_tag="O2692_002395"
     CDS             4592..5332
                     /locus_tag="O2692_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4300001.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGLQQKISDE
                     EINAQLKLVDLEDRGKHFPEQLSGGMKQRVALCRAHVHKPNVISMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            5346..6320
                     /locus_tag="O2692_002396"
     CDS             5346..6320
                     /locus_tag="O2692_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCZ4300002.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKERSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            6317..7078
                     /locus_tag="O2692_002397"
     CDS             6317..7078
                     /locus_tag="O2692_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4300003.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWSFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            7091..8122
                     /locus_tag="O2692_002398"
     CDS             7091..8122
                     /locus_tag="O2692_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCZ4300004.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNVQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
BASE COUNT         2789 a         1205 c         1568 g         2638 t
ORIGIN      
        1 aaaagtgcaa tatgttttcg aaaataagat tatttttatg tgcaaaaacg acgcaaaagt
       61 tttaaaaatg agacttctgt gagctgatta ttttataaaa tgtaaacgct tactatataa
      121 tgtgaatcat atcgtttaaa agcattatta aatatgatgc taagagattt atattatagc
      181 caataaacaa aggagagata atatggcagt aaacgttcga gattatattg cagagaatta
      241 tggtttattt atcaatgggg aatttgttaa aggtagcagt gacgaaacaa tcgaagtgac
      301 taatccagca actggagaaa cactatcaca tattacaaga gcaaaagata aagatgtcga
      361 tcatgcagtc aaagtggcgc aagaggcatt tgaatcatgg tcattaactt ctaaatcaga
      421 acgtgcacaa atgttgcgtg atattggtga taaattaatg gcacaaaaag ataaaattgc
      481 aatgattgaa acattaaata atggtaaacc gattcgtgag acaacagcaa ttgatattcc
      541 atttgctgca agacatttcc attatttcgc aagtgttatt gaaacagaag aaggtacagt
      601 gaatgatatc gataaagaca caatgagtat cgtacgacat gagccgattg gcgtcgtagg
      661 tgctgttgtt gcttggaact tcccaatgct attagctgca tggaagattg cgccagccat
      721 tgctgcaggt aatacaattg tgattcaacc ttcgtcttca acaccattaa gtttattgga
      781 agttgctaaa attttccaag aggtattacc taaaggtgtt gtcaatatac taacgggtaa
      841 aggttcagaa tcaggtaatg caattttcaa tcatgatggt gtagataaat tatcatttac
      901 gggctcaact gatgtaggtt atcaagttgc cgaagctgca gcaaaacatc tagtacccgc
      961 tacattagag cttggtggta aaagcgccaa tatcatatta gatgatgcta atttagacct
     1021 tgcagttgaa ggtattcagt taggtatttt attcaaccaa ggtgaagtat gtagtgcagg
     1081 ttctcgatta ttagttcatg aaaaaattta tgatcaattg gtgccacgtt tacaagaggc
     1141 attttcaaat attaaagttg gaaatccaca agatgaagct acacaaatgg gtagtcaaac
     1201 tggtaaggat caattagata aaattcaatc atatattgat gcagcaaaag aatcagatgc
     1261 acaaatttta gcaggcggtc atcgcttaac tgaaaatgga ttagataaag ggttcttctt
     1321 tgagccgaca ttaattgctg tgccagacaa tcatcacaaa ttagcacaag aagaaatatt
     1381 tggaccagtg ttaacagtga ttaaagtgaa ggacgatcaa gaagcaattg atatagctaa
     1441 tgattctgag tatggtttag caggcggtgt attttctcaa aatatcacac gtgcattaaa
     1501 tattgctaaa gctgtacgta caggacgtat ttggattaac acttacaacc aagtaccaga
     1561 aggcgcacca tttggtggtt ataaaaaatc aggtatcggt cgagaaactt ataaaggtgc
     1621 gttaagtaac tatcaacaag ttaaaaatat ttatattgat acaagcaatg ctttaaaagg
     1681 tttgtactag aataaatatc gtttctgaag cgtgtttgta ggtcagtcta gcggtaagtc
     1741 ttaacattta acggcgttgt ttagatttta agcaaaacaa aatatatagg aacacgtatc
     1801 atgatattag gatataatga ctaaaataat agcagtagga tggtttttaa ttgcaaatca
     1861 tcttactgct gtttttaatt atgctaattt gcgatgcggc tattataagg acagagttgt
     1921 ttattaatta tggtgattta gaaatatgaa gttcaatatg caaagtcatc gtttgtttta
     1981 atatgcggaa caatcattaa agttattgcg attttttgaa cttaatgaaa ctaaacaata
     2041 aatttgagat acttttttgt catttttatg taactaacac aataatctcg tacattatta
     2101 aaattttcta tatgatagga ataaagcaaa gcgcgagtgt gctgtaaaag ttttccaagg
     2161 tgatattaca taaagctata aagggtaaag attaatgagt tgtcatgtaa atgacgatga
     2221 tgtataaatc atggttaatt acggaagcat taatattaac ctgagaagct ataaagaatt
     2281 atttttaaaa gcgacaatat taaatacgac gcatttattt aggagtggca aacgtatgaa
     2341 tgggaaaaag gcgaatacga taaacagata caaatatttt catcatgtca atcatcaaaa
     2401 aattcaacaa agttctaaaa agacgctgtg ggcatcacta atcatcacat tgttatttac
     2461 agtgattgaa tttgtcggag gtttagtatc taattcattg gcattactgt cagattcatt
     2521 tcatatgctt agtgatgtat tagcacttgg tttatctatg ttggccattt attttgcaag
     2581 taaaaagccg actgcacgat acacatttgg atatttaaga tttgagatat tagctgcatt
     2641 tttaaatggt ttagcattaa ttgtaatttc aatctggatt ttatatgaag ctattgtacg
     2701 tattatttat ccgcaaccaa ttgaaagtgg cattatgttt atgattgcta gtattggttt
     2761 actcgtcaat attattttga ctgttatcct tgtaaggtct ttaaaacaag aagacaatat
     2821 caatattcaa agtgcattat ggcatttcat gggagactta ttgaactcta ttggtgtcat
     2881 cgttgcagtt gtattgattt actttacagg atggcgcatc atcgacccaa tcattagtat
     2941 tgtaatttca ctcatcattt tacgtggtgg ttataaaatt acgcgtaatg cgtggttaat
     3001 tttaatggaa agtgtgcctc aacatttgga tactgatcaa attatggcag atattaaaaa
     3061 catagatggc atattagatg tacatgaatt tcatttgtgg agtattacaa cagagcatta
     3121 ttcattaagt gcccatgttg tgttagataa aaaatatgag ggtgatgatt atcaagcgat
     3181 tgatcaagta tcatcattgt tgaaagaaaa atatggcatt gcacattcaa cgttgcaaat
     3241 tgaaaacttg caattgaatc cattagatga gccatacttc gacaaattaa cataaataaa
     3301 acattgtagc gcctaaaaca ttaatctatg tcataggcgc acgtttcgtt ttatacttat
     3361 gttgcatcat ttaaatgatt ttcgtcaatt tctttgatgc tatctacatc taacacgaca
     3421 tctttaggtt tcaaaatatg aatatgtttt tcatcatttg tatgtaaaat gcgttctatg
     3481 atgtaccttt gaccggccat tgtttctaca gcaatctttt tgtttctagc taaacttgct
     3541 acgacagatt ctttatccat aatgatagcc ccctatatat atgtttattt acttataccc
     3601 taacatgatt tttatactct ttgaaaatat attttacaga attttatcta aatatttaaa
     3661 aaatatctta atatccttgt aatccgataa gaattatagt aatatttttt caaccattgt
     3721 tataggaggt cttattaatg acattatttt tattagaagc taacaatctt gattttgcat
     3781 caacgaaaga agaactagaa gcaaaggcag catcactatc tacgaagaca attccaacat
     3841 taattgaagt acaagctact gaaaatttaa ctcatggtta ttttattgtg gaagcaaatg
     3901 acgaagcaga agctaaacaa tttttaacag aagcagatat tagtattcaa ttagtcaaag
     3961 aagtacgctt agttggtaaa gatttagatg aagttaaaaa tggtgatgca catgttgatt
     4021 accttgtaac ttggaacatt ccggaaggca ttacgatgga tcaatattta gcacgtaaaa
     4081 agaaaaattc tgttcattat gaagaagtgc cagaagttga atttaaacgc acatatgtat
     4141 gtgaagatat gtctaaatgt atttgtttat acaacgcacc tgatgaagaa gcggtacgtc
     4201 gcgcgcgcaa agcagttgat acaccgattg atggcatcga aaaactttaa taagacaaca
     4261 agttgatgag atatatgtat ataggtttgg catggatttc gattgcagtt aattagaata
     4321 gctcaatgct ataaatgtaa gtagttgata tgaagaaact aatgaactaa atgcaagtat
     4381 tgtctaaaac aatcatttta ttgaaattta gtagagctga aattaatata acgtcgttaa
     4441 ttgaataacg cttatgttat aagagcactc ataccaaacc ataatcatct atagatataa
     4501 caattcacga tataagggct gtgtttggca tagcccttta gatatacact taattcctat
     4561 taaaatagta ggggttaaaa gggggcttgt catgattaaa attcaacaat tacaacatca
     4621 ctttggatca cataaagtaa ttcataactt taatttggac attagcaagg gagaaatagt
     4681 cactttcata gggaaaagtg gttgcggaaa gtctacttta ctcaatatta tcggtggatt
     4741 tattcatcca tcgtctggtc gtgtcattat tgataacgaa attaaacaac agccatctcc
     4801 agattgttta atgctatttc aacatcataa tttgctgcca tggaaaacga ttaatgacaa
     4861 cattaggatt ggattacaac agaaaattag tgatgaagag attaacgcac agcttaaatt
     4921 agttgattta gaagacaggg gaaagcattt tcccgagcaa ctgtccgggg gtatgaaaca
     4981 acgtgtggca ctatgtcgag cgcatgtgca taagcctaac gttatatcga tggatgagcc
     5041 attaggtgca ttagatgcat ttacacgtta taaacttcag gatcaactag tgcaactaaa
     5101 acataaaacg caatcaacta ttattttagt gacgcatgac attgatgaag ctatttatct
     5161 ttccgaccgc attgttctgt taggtgaagg gtgcaatatt atttctcaat atgaaattac
     5221 agcatcacat ccacgcagtc gtaatgatag ccacctactt aagattcgta atgaaattat
     5281 ggaaacattt gcattgaatc atcatcaagt tgaacctgaa tattatttat aaggagtgag
     5341 tgacgatgaa aaggttaagc ataatcgtca tcattggaat ctttataatt acaggatgtg
     5401 attggcaaag gacgtctaaa gaacggtcta aaaatgccca aaatcagcaa gtgattaaaa
     5461 ttggatattt gccgattaca cattcagcta atttgatgat gactaaaaaa ttattatcac
     5521 aatacaatca tccgaaatat aaactagaat tagttaaatt caataattgg ccagatttaa
     5581 tggacgcatt aaacagtggt cgtattgatg gtgcatcaac tttaatagag ctagcgatga
     5641 aatcaaaaca gaagggctca aatataaagg ctgtggcatt gggccatcat gaaggcaatg
     5701 tcattatggg acaaaaaggt atgcacttaa atgaatttaa taataatggc gatgattacc
     5761 attttggtat accacatcgt tattcaacac attatctttt acttgaggaa ttacgtaaac
     5821 aattaaagat taaaccgggg cattttagct atcatgaaat gtcgccagca gaaatgccag
     5881 ccgcattgag tgaacacaga attacagggt attctgtagc cgaaccattc ggtgcactgg
     5941 gtgaaaagtt aggcaaaggt aagactttga aacatggtga tgacgttata cctgatgcgt
     6001 attgctgtgt gctagtactg agaggggaat tgcttgatca acacaaggat gtagcgcaag
     6061 catttgtaca agattataaa aagtctggct ttaaaatgaa tgatcgcaag caaagtgtag
     6121 acattatgac gcatcatttt aaacaaagtc gtgacgtttt aacacagtca gcggcatgga
     6181 catcctatgg tgatttaaca attaagccat ccggctatca agaaattacg acattggtaa
     6241 aacaacatca tttgtttaat ccacctgcat atgatgactt tgttgaaccg tcattgtata
     6301 aggaggcatc gcgttcatga cacgtcccac aaataacaaa tttatattac ctattatcac
     6361 atttattatt ttcttaggca tttgggaaat ggtcattatt attgggcatt accaacctgt
     6421 attgttaccg ggtcctgctc ttgtaggaaa aagtatatgg tctttcattg ttactggaga
     6481 aattttccaa catttagcaa ttagtttatg gagatttgta gcgggctttg ttgtcgcatt
     6541 gttggttgct attccattgg gcttcttgct tggaaggaat cgttggctat acaacgctat
     6601 cgaaccgcta tttcaattga ttaggccgat atctccgata gcatgggcac catttgttgt
     6661 tctatggttt ggtattggta gtttgccagc gattgcgatt atttttatcg ctgctttttt
     6721 cccaattgtg ttcaatacta ttaaaggcgt tagagacatt gaacctcaat atttaaaaat
     6781 agcagcaaat ttaaatttaa ctgggtggtc attgtatcgc aatatattat ttcccggggc
     6841 atttaaacaa atcatggctg ggatacatat ggcggtagga acaagttgga tatttttagt
     6901 ttctggtgaa atgattggtg cacaatcggg attaggtttt ttaatcgttg atgcacgaaa
     6961 tatgttgaac ttagaagatg ttttagcagc aatattcttt atcggattat ttggttttat
     7021 tattgatcga ttcattagtt atattgagca gtttatactt agaagatttg gtgaataagg
     7081 agagatgatg atgactttag aaacgcttat caaagaacaa ttagatcctc atttagtaga
     7141 agttgatgaa gggacgtatt atccgagaac atttattcag caattatttg tagatggtta
     7201 tttcggtgag gcggcattga gaaaaaatgc tgaagtaatc gaagctgtat cgcagtcttg
     7261 tttgacaaca ggattttgtt tatggtgcca attagctttt tcaacgtatt tagaaaatgc
     7321 cacgcagcca catttaaata atgacttaca acagcaattg ttatctggag aaatattagg
     7381 tgctaccgga ttgtctaatc cgatgaagtc atttaatgat ttagaaaagt tgaaccttga
     7441 acacacttat gttgatggac aattggttgt cagtggacgt atgccagctg taagtaatat
     7501 tcaagaagac cattattttg gtgcgatttc gaaacatgaa tcatcagatg aatttgtcat
     7561 gttcattcta cgtgccaatc aagatggtat cactcttgtt gaaaaaacca attttttagg
     7621 agtcaacggg tcagcaacgt atcaaatcac attgaatcaa gtcgtagtgc cacaatcaca
     7681 aattatcacg catgatgcga agcagtttgc ggcaactatt cgcccgcaat ttattgctta
     7741 ccaaattcca ataggattag gctcaattaa aagttcttta gagttaattg atgcattttc
     7801 aaatgtgcaa aacggaataa atcaatattt agagtatgat gttgaagctt ttaaaaaacg
     7861 ttatcgtcaa cttagagagg aatattatgc aatattagat gacggtaact taacttcaca
     7921 tttaaatgaa ttaatatcat tgaagaagga catcggctat ttattgttag atgtaaatca
     7981 agcttctgtt gtcaatggtg gttctagagc gtacacacca tattcgccac aagttcgcaa
     8041 gttaaaagaa ggattcttct tcgcagcatt gacaccgaca ttaagacatt taggtaaact
     8101 tgaagcagag ttgaaggggt aagtgtgata agctgatttt ttgtttagat gcgtttgttg
     8161 aaacattttt taaaataata taaatcttag tttataaaca
//
LOCUS       DALAGF010000120         8025 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.120, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000120 DALAGF010000000
VERSION     DALAGF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8025
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.120"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            86..1336
                     /locus_tag="O2692_002399"
     CDS             86..1336
                     /locus_tag="O2692_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCZ4300005.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            1519..2469
                     /locus_tag="O2692_002400"
     CDS             1519..2469
                     /locus_tag="O2692_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCZ4300006.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            2618..4102
                     /locus_tag="O2692_002401"
     CDS             2618..4102
                     /locus_tag="O2692_002401"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCZ4300007.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYDEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            4099..5058
                     /locus_tag="O2692_002402"
     CDS             4099..5058
                     /locus_tag="O2692_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCZ4300008.1"
                     /translation="MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(5246..5353)
                     /locus_tag="O2692_002403"
     CDS             complement(5246..5353)
                     /locus_tag="O2692_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300009.1"
                     /translation="MHCIAGFLILLTKLNMTRELKNDVTSLCIPIPITT"
     gene            complement(5425..6141)
                     /locus_tag="O2692_002404"
     CDS             complement(5425..6141)
                     /locus_tag="O2692_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4300010.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(6160..7452)
                     /locus_tag="O2692_002405"
     CDS             complement(6160..7452)
                     /locus_tag="O2692_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCZ4300011.1"
                     /translation="MELLNSYNFVLFVLTQMILMFTIPAIISGIKYSKLDYFFIIVIS
                     TLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAY
                     ITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLL
                     TFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIE
                     TYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMPGLRDYFNKNIVPMKDNLQ
                     MNAIKLNGIENLKVREIKGLITAKILRAQEMNFPISIEIPDEVSSINLNMIDLSRSIG
                     IILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEG
                     RGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN"
     gene            complement(7477..7617)
                     /locus_tag="O2692_002406"
     CDS             complement(7477..7617)
                     /locus_tag="O2692_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HCZ4300012.1"
                     /translation="MNTLFNLFFDFITGILKNIGNIAAYSTCDFIMDEVEVPKELTQL
                     HE"
     gene            complement(7614..>8025)
                     /gene="agrB"
                     /locus_tag="O2692_002407"
     CDS             complement(7614..>8025)
                     /gene="agrB"
                     /locus_tag="O2692_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HCZ4300013.1"
                     /translation="NIFLFTLITNLTFYLIRRHAHGAHAPSSFWCYVESIILFILLPL
                     VIVNFHINFLIMIILTVISLGVISVYAPAATKKKPIPVRLIKRKKYYAIIVSLTLFII
                     TLIIKEPFAQFIQLGIIIEAITLLPIFFIKEDLK"
BASE COUNT         2850 a         1095 c         1401 g         2679 t
ORIGIN      
        1 tttattccaa aagatgtaat aaaacttaac gcatttttgc tttttataaa ttgtcagatt
       61 attatgaaaa aaaggagtgg taagtatgaa tcttaacgat acgatattta tgtttttgtg
      121 tacattatta gtttggttaa tgacaccagg attaagttta ttttatggtg ggttagttca
      181 atctaaaaat gcgcttaata ctgtcatgca aagtatggca gcaattgtgc ttgttacatt
      241 tgtatggata acagttggtt ttacaattag ttttgggaat gggaatttat ggttcggaaa
      301 ttgggaatat acttttctta atcatgtagg ttttgcgact caagaagata ttagcccaca
      361 tattcctttc gctttgttta tgttatttca aatgatgttt tgtacgattg caatttctat
      421 tttatctggt tcaatcgctg agaaaatgaa gtttattcct tatttattat tcgtagtaat
      481 atggactgct cttgtataca gtccagtagc acattgggtt tggggcggcg gttggattaa
      541 caaactcggt gtattagatt tcgctggagg tacggttgtt catattacat caggtgtttc
      601 tggtttagta ttagctatta tgattggaaa aggaaacaaa cattctgaat caacaccaca
      661 taatcttatc attacgttga ttggcggtat attcgtgtgg attggttggt atggatttaa
      721 tgtaggtagt gcttttacat ttgataatat tgcgatgctt gcatttacaa atactgtcat
      781 ttcagccagt gcaggtgcta taggttggtt aattttagaa tatattttta aaaagacgac
      841 aagtttactt ggacttttac tcggtgcatt agcaggatta gttgtcatta ctcctgcagc
      901 aggatatgta acatatctta gtgcaacaat aatggcttta ataggaggta tctgttgtta
      961 tattgtcatt aattacatca aggtaaaact aaaatatcat gatgcattag atgcatttgg
     1021 tattcatggt gttggtggta ttattggtgc tgttttaaca gcagttttcc aaagtaaaaa
     1081 agccaatcct gacattgaga atggctttat ttatactggt gacatacata ttatacttgt
     1141 acaaatatta tgtgtaacag cagttgtaat ttttagtata gtcatgacgt ttattattgc
     1201 gaaagtaatt aaattaatta caccattatc tgttacggaa caagaaacga atataggatt
     1261 agacaagatt gttcacggtg aacatgctta ctttgaaggt gagctaaata gattcaataa
     1321 acatattcga tattagaata tatttacata gaatattcat tgtcctgaca tttaactaaa
     1381 ggttgatgtt gggacatttt gttatacaaa agttttattt tgaaatcttt ttatgaaaga
     1441 agcagaaata ttatttaaag cggttacaca tatgctaaaa taaggctaag tgtcacaaat
     1501 aatgataggt gaataagtat gaaaaatata tctgatattg ccaaattggc aggcgtttca
     1561 aaaagtacag tatctagatt tttaaataat ggatctgtca gtaaaaaaac aagtgaaaaa
     1621 ttaacaagaa ttatagcaga acatgactat caaccgaatc aatttgctca aagtttaaga
     1681 gcgagacaaa cacaccttat tggtgccatt atacctagaa tgaattcata tgcagtagat
     1741 gaaacaatca aaggattggc aaaacaatgc caaaaatatg aatcacaatt aattttaaat
     1801 tacacaggtt taaatatcga agcagaaata caagcgcttg aaacattagc acgcagtaaa
     1861 gtagatggta ttgttttaat ggctacagac ataacagaga gacatattga agtcattaat
     1921 aaaatgaatg taccaatcgt tattgttggt caacaacatg aacaacttca tagtattgtg
     1981 catgatgatt ataaagcagg tcaaattata ggcgaatgga ttggtcaaca gggatatcag
     2041 caagttgaag tgtttagtgt aagtgaaaaa gatattgcag ttggtataca tagaaaacgt
     2101 ggtttacttg accagttagc taaataccaa attaaaccta atatacatga aacaaatttt
     2161 acttatgtgg aagcacaaaa agatgttgca aatgttttgg aaaatgtgga gcaagtagat
     2221 gcggttgttg gagcaactga tacgattgca ttagctgcct ataaatatta ttctgataaa
     2281 aaagatgtta tgaaaccaca tcaaatatat ggttttggtg gtgacccaat gacacaatta
     2341 gtgtctccat cgataaaaac aattcattat aattattttg aagctggcca atgcgcgatg
     2401 gaagagatac aacagatgct taaaaagcaa gatatgccat atagcgtcac agtagatgtt
     2461 aatatttaga cgctgtattt tttaaaataa atgtggaacc gataccatat aactataaat
     2521 ggataggtta aaagttaaag aacgtaggta aaatttgcta taatagaata taaattgtta
     2581 ttagcataaa ttataaaagg aggactgggt aaatattatg accgaatgga ctagagaaga
     2641 acgttatcaa cgaatcgagg acgttgatac tgagtatttt aaaacattaa aacaacaagt
     2701 tgatcaatca aaatttcgtc aacaatttca tatacaacca gaaacaggct tattaaatga
     2761 ccccaacgga cttatttttt ataaagggaa gtattatgtt tcacatcaat ggttcccatt
     2821 aggcgcagta catggcttaa agtattggta taactacacg agtgatgact taataaactt
     2881 taaagctgaa gggccaattt taaatccaga tactaaatat gacagccatg gtgtatatag
     2941 cggtagcgct tttgaatata acgggcattt atattatatg tacacaggaa atcatcgaga
     3001 taatcattgg caacgacatg cgagtcagat gatcgcacga ttgaaagaag acggttcagt
     3061 tgaaaagttt ccaaagccag taattagcca acaaccagaa ggatatacga gtcattttag
     3121 agatcctaaa gtttttaaat atgatgagaa atattatgca atcattggtg cacaaaataa
     3181 tgatcagcaa ggtcgattat tactttataa tactgaagat ataattaatt ggcattattt
     3241 aggtgaaata aatacagagt tggatgattt tggatatatg tgggaatgcc cagattactt
     3301 taatgtagat aatcaagatg tcatacttat ttgtccacaa ggtattgaac caaaaggcga
     3361 tcagttcaaa aatatttatc aaagtggtta tatacttgga aagtttgata ttgaaaagtt
     3421 aacatatgaa catgaaaatt ttgtcgagct tgataatggt tttgatttct atgcacctca
     3481 aacattttta gatgaaaaag gccgacgagt actaattgga tggatggggt taccggaaat
     3541 cgaatatcct actgataatg aaggatgggc ccattgctta acaattcctc gtgtattaaa
     3601 tgtagaaaat ggtcaactta agcaacgtcc atatccagcg ttggaaaaat tacgtcacaa
     3661 taaagagaca gcattaggct acgcaaataa atttactcga aaattacatc cgtatgaagg
     3721 taaacagtat gaattaatca tagatatttt ggataatgat gctaccgaag tgtactttga
     3781 attacgtaca tctaagactt cttcaacatt aattgcttat aacaagcgtg aaaataaaat
     3841 aacattagat cgcagcgaca gtggtttatt gccgacaaat gttgaaggta cgacgcgtag
     3901 tacgatatta gacacgccat taaagcaatt acaaattttt gttgatacat ctagtataga
     3961 aattttctgt aatgatggag aacgtgtttt gacatctcga attttcccga cagaagacgc
     4021 actaggtatt aaaacatcaa cagaatcagg gcaagtatat ttacaattta ctaaatatga
     4081 tttaaaggat gaacataaat gagacgtcta ttttcaatag gagaagcgtt aattgatttt
     4141 attccaaatg ttacaaatgc gaatttaaaa gatgttcaaa catttacgaa gcaaattggt
     4201 ggcgcgccat gcaatgtagc ttgtacagtt caaaagttag gacaacaagc gtatatgatt
     4261 acacaattag gtaatgatgc atttggagat agcattattg aaacgatatc aagtattggt
     4321 gtagatgtat caaaagtgta tcgcacgaat gaagcaaaca ctgcgttagc ttttgtgagt
     4381 ttgactgaag cgggcgagcg agatttttct ttttatcgta agccctctgc tgatatgtta
     4441 tttgaaccaa gttttgtgaa tgatattgat gtgaatgaaa atgatgtagt acatttttgt
     4501 tctgttgatt tggtcgatag tccgatgaga gatgcacatt atcaattgat taccaaaaca
     4561 ttaaatgcaa atggtactgt cgtatttgat cctaatgtaa gattgccatt atgggataac
     4621 gctgaagatt taagacaaac tattcatacc tttttgccat tggcgcatat tgtaaaagtt
     4681 tcagatgaag aattagaatt tattacaggt attcatgatg aaaatgaagc cattcaatca
     4741 ttatttacag gtaatgttac tgtagtcatt tatacgaagg gagcagatgg tgctgccgta
     4801 tatttgaaaa atggtattaa tcattatcat agtggctata aggtaaaacc tgttgataca
     4861 actggggcag gggatgcatt tattggtgca gttattagca ggattttagc aaccgatgtg
     4921 ttaaatttaa ctcagttatt tgaaaatgaa ggtgaggaaa ttttagcgtt tagcaatcgc
     4981 gttgctgcaa tagtgacaac gaagtatggt gctattaata gtttgccaac attacaagag
     5041 gttgaacaag cattttaaat aattcattta taaaacagtg taggaaacaa gttgaatctt
     5101 gtgctacact gttttttaaa acaaaataag attcacggag taggaaatta tagcatttta
     5161 tttatggaaa ctgtcaattt caaatttagt atattttgtt aaaaattgcg ccataggatt
     5221 gtagagtgaa atagaaattg ataaattatg tagtaatagg aataggtata caaagagacg
     5281 ttacatcatt ttttaattca cgcgtcatat ttaattttgt taataaaatt aaaaagccag
     5341 ctatacagtg catttgctag ttatcttgtt aaaatccaac aagatttaca attgaatacg
     5401 ccgttaactg actttattat cttattatat ttttttaacg tttctcaccg atgcatagca
     5461 gtgttcttta tttttaaaat agacaattcg ctctttcgaa tctatagatt caatattatg
     5521 gcgattgacg acaaagctat tatgacatct aaagaaacga tcatctaatt gactcagttc
     5581 ttttaaatta ccataaaatt caatttgacg gttatctaaa tgggcaatga gtctgtgaga
     5641 ttttgttgat gattcaaaaa acataatatc atcatattga acatacactg aattactgcc
     5701 acgttttaat tcaatcgttt caacgctatt atctttagac aacaattgta agcgtgtatg
     5761 tgcagtttct aaacagtcta taattcgagt tcttaattca gctggatcat ctttaaaaat
     5821 aaaatccatc gctgcaactt tgtagacaaa tgttaaatag gtaagttcac tgtgactcgt
     5881 aacgaaaata atgttaccaa ctgggtcatg cttacgaatt tcactgccta atttgatacc
     5941 attaatatca gttgaaagtt gaatatctaa aaagtaacag cctatgtcat tcatattttt
     6001 agcttgctca agcacctcat aaggattatc agttgcgagg gcaatttcca taggcttttc
     6061 ttctatcatt atataatttt taataatggt aaccatgttt tctctttgtt ttggatcgtc
     6121 ttcgcaaatg aaaattttca tacattcaca tccttatggc tagttgttaa taatttcaac
     6181 tttttgaata aagaaaccat tttcgataat tgtatctaat aagacattgt ctgcattatc
     6241 agcaatttct tttaaagttg atagacctaa accacgacct tcacctttag tagaaaaact
     6301 ttcttggaac aattcatgaa tgcgtggtat atcatcagcg catttattca taacaataaa
     6361 cgttactgaa ttttcacttt caataaatgc aacgcgaatg atagggtcat caatttcagt
     6421 tgatgcctca attgcattat caagaataat gccaatactg cgacttaaat cgatcatatt
     6481 caagttaatg ctacttactt catcgggtat ttcgatacta atcggaaaat tcatttcttg
     6541 tgcacgtaaa attttcgcag taattaagcc tttaatttca cgtactttaa gattctcgat
     6601 accatttaat tttatagcat tcatttgtaa attgtctttc ataggtacaa tatttttatt
     6661 gaaataatca cgtaggccag gcatgtcatc ttctcgaatg tattctgaaa gtgtcgttaa
     6721 gatattgaca taatcatgac ggaacttgcg catttcgttg ttgatagctt caatcttcaa
     6781 tgtatattca taataggttt caatttcttc ttgattacgt ttatatttca tctctttaag
     6841 gagaaattga gaaataacaa atgttaatat acttaaaaat atagtgatac caataaaaat
     6901 aaaagaatac tgccttatta ctttagcttc atccgagttt atttgtgaat aaaagaaaaa
     6961 taatgaaaaa gtaagcagta agatagtcga aataactatt aaaaatcctt tgtttagtat
     7021 tagatatggt gtgctaattt ttttgagaac tctatttatt atatatgaga atagtatact
     7081 aatagtcaca taaactacaa aaaagctagg gaatattaca aatatactat cagaaatttt
     7141 ggtgatatat gcatatataa ctatatacat gtagttagca cagtatagaa taatctgcga
     7201 agtcataatc aacaaaatag aataccattt gattttgaca aaatacatta taataataaa
     7261 tgaagttaat atgattaagg acgcgctatc aaacatttta aatagaaata acgataatgt
     7321 cgaaattact atgatgaaaa aataatcaag tttactgtac ttaataccac taattatagc
     7381 tggtattgta aacattaata tcatttgagt taatacgaat aaaacaaaat tataactatt
     7441 taataattcc acctactatc acactctcta tttaaattat tcgtgtaatt gtgttaattc
     7501 ttttggtact tcaacttcat ccattatgaa gtcacaagta ctataagctg cgatgttacc
     7561 aatgtttttt aaaatcccag taataaaatc aaaaaataag ttaaataatg tattcatttt
     7621 aagtcctcct taataaagaa aataggtaat aatgtaatag cttctattat gatgcctaat
     7681 tgaatgaatt gggcaaatgg ctctttgatg ataagtgtga taatgaaaag ggttaaacta
     7741 acaataatcg cataatattt ttttcgttta ataagtcgca caggaatggg cttcttttta
     7801 gttgctgcag gagcatatac tgagattaca cctaaagaaa taactgttaa aataatcata
     7861 attaaaaagt taatatgaaa atttactatt actaaaggta aaagtataaa tagtataata
     7921 ctttctacat aacaccaaaa agaagaaggt gcatgtgcac catgtgcatg tcttcttatt
     7981 aaataaaatg ttaaattcgt aattaacgta aacagaaaaa tgttt
//
LOCUS       DALAGF010000121         7928 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.121, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000121 DALAGF010000000
VERSION     DALAGF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7928
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.121"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            652..1029
                     /locus_tag="O2692_002408"
     CDS             652..1029
                     /locus_tag="O2692_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCZ4300014.1"
                     /translation="MNKKYLMIVIIILILILGGIFLKMKYDEKKYYDEQKERITIYMK
                     YNVKGYKNISFANFKENPMDGYSISGYINNDKKLSFTAGIRSVDDFQFDTDISYTDEL
                     GRKFNKNPKSVSEIKKEQNTSNK"
     gene            1223..1399
                     /locus_tag="O2692_002409"
                     /pseudo
     CDS             1223..1399
                     /locus_tag="O2692_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1377..1478)
                     /locus_tag="O2692_002410"
     CDS             complement(1377..1478)
                     /locus_tag="O2692_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300015.1"
                     /translation="MQGFKEKHQELKKALCQIGLMRSISEVKQLNIA"
     gene            complement(1601..1696)
                     /gene="pepA1"
                     /locus_tag="O2692_002411"
     CDS             complement(1601..1696)
                     /gene="pepA1"
                     /locus_tag="O2692_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HCZ4300016.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            1899..2042
                     /locus_tag="O2692_002412"
     CDS             1899..2042
                     /locus_tag="O2692_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCZ4300017.1"
                     /translation="MTRERRSFSSEFKLQMVRLYKNGKPRNEIIREYDFTPSTFVNGG
                     YKM"
     gene            2646..3029
                     /locus_tag="O2692_002413"
     CDS             2646..3029
                     /locus_tag="O2692_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300018.1"
                     /translation="MSNQNLFDELEKKGYKLEDIFTKEEIKKYKAEDQLRAGKTQYVE
                     TGKDTATLYLSSAYTKTIAALGAGAISVISALTGGLVGAGVGGFLGSIAASNIDTSKG
                     IYIKLKTKKNAAGEYVLTGEKWGYQ"
     gene            3040..3216
                     /locus_tag="O2692_002414"
     CDS             3040..3216
                     /locus_tag="O2692_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300019.1"
                     /translation="MDRLKYSLKVGILALLLFCTLNYLVPMQSNAFSIIIYSAIFAVL
                     LMLLVYIFLGILKK"
     gene            3218..3403
                     /locus_tag="O2692_002415"
     CDS             3218..3403
                     /locus_tag="O2692_002415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300020.1"
                     /translation="MKQISIGYEEIYGDRIFHSHFLKEHEVKNWKTVNQIIEKEHLDK
                     NEYEAEPPLFFFSGSLD"
     gene            3517..4158
                     /locus_tag="O2692_002416"
     CDS             3517..4158
                     /locus_tag="O2692_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCZ4300021.1"
                     /translation="MFTPIVINLLNDVGSIFLVRVGNQLKVIEKVHYEDILESKIVRL
                     FQAFIKDKMDQLFITSEHFAIDIDKLLFQLNLNVRYIKMEGNTSGYLSGRTIYINNNH
                     SINNTRFSIARELGRYLFKNTDLMRDSNDNSLKNLHEMIYESDVNQFSVDLLMPKKQI
                     EALVYNFYEVNNINLSSGLSEKERNKLLNLISTKLEVSKVAAGLRLYNLGIHI"
     gene            complement(4376..4927)
                     /locus_tag="O2692_002417"
     CDS             complement(4376..4927)
                     /locus_tag="O2692_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300022.1"
                     /translation="MEKNEYIAKYNEYSQLLDATYSQAVAYLLSKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISKFKYSYDVQKKENL
                     VYCDLIEHLILHAIITKESHGQFGVAGLCQMIKPTVIDWYIGEYNPKPAWMQATKARA
                     YLPGILVEKLLIKIDDMLKGIEI"
     gene            complement(5025..5369)
                     /locus_tag="O2692_002418"
     CDS             complement(5025..5369)
                     /locus_tag="O2692_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HCZ4300023.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIENAEHTLFTPYM
                     LETLSSLGVKDSIVDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLKQYKNVEFND
                     KILINWRIIQEK"
     gene            complement(5410..6036)
                     /locus_tag="O2692_002419"
                     /pseudo
     CDS             complement(5410..6036)
                     /locus_tag="O2692_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6112..7107)
                     /locus_tag="O2692_002420"
     CDS             complement(6112..7107)
                     /locus_tag="O2692_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCZ4300024.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVSYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDKSSVEKSESNEETAPVEPMPHSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            complement(7188..7838)
                     /locus_tag="O2692_002421"
     CDS             complement(7188..7838)
                     /locus_tag="O2692_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HCZ4300025.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSVKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTNNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTE
                     EVPANENTENNQHEIDEITTKDQSDDDINTPNVAEDKSQDDLKDDLKEKQQSSNHHQS
                     TQPKTSPSTETNTQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
BASE COUNT         2655 a         1126 c         1268 g         2879 t
ORIGIN      
        1 ggaaaatata tctagaaaaa atttctcttc gagatagaca agaatggtgg tacaatgttt
       61 tttatgacga tggcaaatat aataagaaaa taattaagaa tgacatgtca aaaattaggc
      121 gtaattttta gatttatcta tatatttatg ccaccgctca aacttttata aaaagtaaaa
      181 aaacaatatt tatattgaac ttgtgacgtg aatttctaaa acacaaccaa caatccacaa
      241 ggaaactagt aaataagttt aaataagatt tcgaaaaaat gtgtaatatt ataatatgaa
      301 aatctttaag attatagaag aatcatactg actaaaaaga ttttgaaagt gattaagtat
      361 ttaaaacacg ccaatcttaa accgctattg acaaatatga agcggaacca ccgttgtttt
      421 tcttttcagg tccaattgaa taaatgttat gaatagaatg gataaaaata taattattta
      481 agtgttaaag taaaccaaat catgatttaa gattataaga aagaaaaatt gactaatgtt
      541 aaaaccaaca atcatatggg ttgtaacgtt tatacaatca tcgtaaaact caatatgctt
      601 aatttattca aaatgttcgt caataagtac aatgaaaagg agattgattt tttgaataaa
      661 aaatatctta tgattgtaat tataatttta atattgattc taggcggaat ctttttgaag
      721 atgaaatatg acgaaaagaa atattatgat gaacaaaaag aaagaataac gatttatatg
      781 aagtacaatg tgaaaggtta taaaaatata agcttcgcta attttaaaga aaacccaatg
      841 gatggttatt ctattagtgg ttatataaat aatgataaaa agttatcatt tacagctggt
      901 ataagatctg ttgatgattt tcaatttgat accgatattt cttatacaga tgaattgggt
      961 agaaaattta ataaaaatcc taagtcagtt tctgaaataa aaaaagagca aaatacgtcc
     1021 aataaataat tgttcatatt gtgatgaaac aaaaatataa gtcattagat gagtttaact
     1081 atgttataaa tatttgtagt atctataaaa atctcgacac tattaaaatg ataaagtgcc
     1141 gaggttttct tacttattta gttaattcaa agtttatgcc agattcataa gaatttgtga
     1201 cacttttaat agtgtaacca ttgattatta caatttatca aatggtcctt tagaaggtat
     1261 aaataacaaa ataaaattaa ttaaacgtac atcttttggt tatggaagtt acaatcattt
     1321 gcgcaatcga atattattat gttcaaaact ttacgctcca aaaagtaaaa aggaagttaa
     1381 gcaatgttta gttgcttaac ttcggatatt gaacgcatca gtccaatttg acatagagcc
     1441 ttttttagtt cttgatgttt ctctttaaaa ccttgcatat tttacaaaaa gaaaaattag
     1501 cagtataatt aagacaacga aaataagtat ttacttatac accaatcccc tcactatttg
     1561 cggtagtgag gggattttta ttggtgcggc tatatgtcac ctattttgta ttgcgtctac
     1621 ttagccaata agaaaaaaac gcaatggcac agccactgat gactggtgct atgatgtgaa
     1681 cgaaaataag catcacctta tacacctcct ctctgcgtct aaattgacgc ctgagagata
     1741 ggcgactcta ctattatatc atcggcaaat atacaagcac agtcacttgc ttctgataag
     1801 ttatatgatt ctagctgata gattgaatcg tctacactta attggacaaa ttctatgaga
     1861 atagatattg ttaatttaag aaagtaggcg attttattat gacaagagaa agaagatcat
     1921 ttagttcaga gtttaagtta caaatggtta gattatataa aaatggtaag cctaggaatg
     1981 aaattatacg cgagtatgat ttcacacctt cgacgtttgt aaatggcggt tataaaatgt
     2041 aggaaaatgg ataaagcaac atcaaaacac gggtacattc aatcaccaag ataacttatc
     2101 ggatgaagaa aaagagctga ttaaattacg caaagaagtt caacatttaa aaatggagaa
     2161 cgatatttta aagcaagtag cgctgattat ggggcaaaaa tagaagtcat tcaaaagaat
     2221 gcacatcaat attcagtatc agcaatgtgt aaagtcctga taatactaag aagtacctat
     2281 tatgattcta taaaaagaaa agataataaa atcactaaag atgattcaaa catagaacat
     2341 gccgtcataa atatttttaa ttctaataga aaagtctttg gtacaagacg aattaaaaat
     2401 catttaaatg acaagggtct cactgtatct ggacaaaaga taggtcgatc atgaaaaaat
     2461 ctagtttctg tttatacgaa atctaaatac aaaaatcatc taaaagaaac taatgaaaaa
     2521 cgaattaaaa atctttatta ttagctgctg gtgtattatt tgttagtcca atttcattat
     2581 cttataattc agatgtagct catgctgaag ataagttaga ccattctcaa gcaaaggtaa
     2641 tatatttgag taaccaaaat ttatttgatg aacttgagaa aaaaggttat aaactggaag
     2701 atatatttac aaaagaagaa ataaaaaaat ataaagctga agaccaattg agagcgggta
     2761 aaactcaata tgtagaaaca ggtaaagata ctgcaacatt atatctttct tctgcatata
     2821 caaaaacaat agctgcttta ggtgcggggg ctattagcgt tatttctgca ctaacaggag
     2881 gtttagttgg cgcaggtgtt ggtggtttcc ttggatctat agctgcatcg aatattgata
     2941 ctagtaaggg aatatatata aaattaaaaa ctaaaaagaa tgcagccggg gaatacgttc
     3001 tgacaggaga aaaatgggga tatcagtaag ggtgatttta tggatagatt aaaatattca
     3061 cttaaagttg gaattttagc attattatta ttttgtactt taaattattt agttccaatg
     3121 caaagcaatg ctttttcaat aattatatat tcggcaattt ttgctgtgtt acttatgctt
     3181 ttagtttata tatttttagg aattttaaag aaatgacatg aaacaaatta gcattggcta
     3241 tgaagaaatc tatggggata gaatttttca tagccatttt ttaaaagagc atgaagtaaa
     3301 aaattggaaa accgtcaatc aaataattga aaaagaacac ttggacaaaa atgaatatga
     3361 agcggaaccg ccgttatttt tcttttcagg atcgttagat tagattcggt tgtatagtat
     3421 aatgaaaaat attataataa atttataaaa aaggtatttt attttgaaat catgtccctt
     3481 cgttacatat agtagtagag gggttttatt ttaataatgt ttacacctat agttataaat
     3541 ttattaaatg atgtaggttc gattttttta gttagggtgg ggaatcaatt gaaagttatt
     3601 gagaaagttc attatgaaga tattttagaa tcaaaaatag ttcgattatt tcaggcattt
     3661 ataaaggaca aaatggatca actttttatt acttctgaac actttgctat tgacatagac
     3721 aagctattat ttcaacttaa tttaaatgtt agatatatta aaatggaggg taatacttcg
     3781 ggttatttaa gtggaagaac aatctatatt aataataatc actcgattaa taatacaaga
     3841 ttttcaatag ctcgtgaatt gggccgttat ttatttaaaa atacagactt aatgagagat
     3901 agtaacgata atagtctaaa gaatttacat gaaatgattt atgaaagcga cgtaaatcag
     3961 ttttcagtcg acttacttat gcctaaaaag caaatagagg cattagtcta taatttttat
     4021 gaagtcaata atatcaattt aagttctggt ttatctgaaa aagaacgaaa caaactattg
     4081 aaccttatat caactaagtt ggaagtttcg aaagtagctg ctggattaag gctgtataat
     4141 ttaggaatac atatttgata aatattgtga aaatctagtc gatttattaa aaccaaaaga
     4201 aatttaatac aattcaagac catgcaaact catttataaa cattgtccgt ttatagatag
     4261 gtatgcgtgg tctattttat aaaatatatt taattacagg tttaaacatc tataacaaaa
     4321 tctcaacagc aaaaaataaa tctacattaa tcatttatct agactcaagg aaatcttata
     4381 tttctattcc ttttaacata tcgtcaattt taatgagtaa tttctctact aatattccag
     4441 gcaaataggc acgtgctttg gtggcttgca tccatgctgg ttttggatta tattcgccaa
     4501 tgtaccaatc aatgactgtt ggtttgatca tttgacataa tccagctaca ccaaattggc
     4561 catgggattc ttttgtaata attgcatgta aaattaaatg ctcgattaga tcacagtaca
     4621 ctaagttttc tttcttttga acgtcgtatg agtacttaaa tttggaaatg aatcttagat
     4681 cagatagatt ttggaatttg tcctcgctta tatgatggca atataatcct tcgctagctc
     4741 tagtgtattt tccttttgaa atacttttga tttctccatt taaaaatcgc gtgtatgatt
     4801 tttctttata ataatcatcg gttacagcgc catatttact taaaaggtat gctacagctt
     4861 gcgagtatgt agcgtctaat aattgactat attcattata tttagctata tattcatttt
     4921 tctccatttc aatatacctc ttttcaaact ttataatatg aaatgcttgt aatttacaaa
     4981 gaataaaatc aatactcacg tagttacatt cgacttttat atgtctattt ctcttgtata
     5041 atacgccaat tgattaatat tttgtcattg aattccacat ttttgtattg ttttaatagt
     5101 tcttcagttc tttgtaaaca gactgtaact gtgtcttcaa ttgataaatt aaatgccgca
     5161 aagtcttcta attcagtccc tttatgaatt aaatcgacaa tgctgtcttt cacgcctagg
     5221 gaagagagcg tttccaacat ataaggtgta aaaagcgtat gctctgcgtt ttcgattgaa
     5281 ataatgccga gtttaagttg attaaaaaga ccatgcatag tcattaaggt gtatttctca
     5341 agttgttgta tattctcaaa tgttatcata gcgtttctcc taaaaattac gattaatcaa
     5401 agtatgccat tatttaactt tagtttcttc agaattattt gccccattat catgtttttc
     5461 ttctttatta acagcatttt tatcattttt atcgtcgact ttttgatctg gtatgtcatt
     5521 ttgaattgct ttgttattat caatgtcatg atgttgaatg tcttctgact caccatctgt
     5581 ttcatcatga ttgttcatac ctggattgta tttagggctt ctctttttaa atgcttcaaa
     5641 ttcttctttg tctttttctg acatatcatc aaaaatatta ataattgtgc cattgtgtcg
     5701 attaacaata gcacaacgtt ttaatggtgt gccaactgca tcgcggacta gatgttcaac
     5761 ataatattca tgttcattgc ttcgttcaag atttgtttta taatttaatg tttgatattc
     5821 attaacgcct cttgctttgt aatcgtttcg aactatatct tcggcttctt gtggtgtcat
     5881 ttacttttct gtatttgact gtgtagtgtt gtcagtttgt tgcgtctttt tattagtgtc
     5941 atttgattta ctactatttt ctttatgttg attagcacca caggcagtta atagtgacaa
     6001 tgataatgtg attgcaacga tagctttgaa tttcattttt ttatctcctt gatttgatta
     6061 tgtataatta atttttccgt aaaagaaggg tagccgagct accctgaaat tttattctgt
     6121 tgaagcgtcg tacatgctat cttcattgtg aatattttgg ctaggtggtg cttcactagt
     6181 ggtaggtttg ctatggggca tgggctctac aggagcagtt tcctcattag attcactttt
     6241 ttctacacta gatttatcac ttttagacgc attctgctta tcctcatctt tactagactt
     6301 ttcgctatct tcagaatcag tatccgcact agaagttgta gcaggtgtag tagccgaagc
     6361 ttcataactt acattatcgc tatttgaact cgcaacagct acttctgtat cttgcttgtc
     6421 ttttttagct tttgatgttt ttttagcatc ggataaatca aatgtaatct tcttgacgct
     6481 aaataaacta gaaataactt ctaatttttt atcttttgcg ttggctatgt tttctgacac
     6541 atcgaaaaca attttacctt gagcagtgct atctggattt atacgctgta agaaaaatga
     6601 attttctata ctaccattgt cactttgatt agcagacatt gaacctgtat tatctgcttc
     6661 aaatgtttta tcaccggatt ttagcttaaa cattgaacta tcaattgtta acgcttcttt
     6721 acctttgttt ttaatcgtca catcagcaac gacaaatata cctttagcgt ttgttggtgc
     6781 aagagatggt cctacagatt tcatagtttc cactgaattt acagtgactt caaggtcgcc
     6841 atttttaaca gtttcaccga ttttatgggt tttgttcgtt tctttagaac tatttccgcc
     6901 aaggttacct gtaataccag ctgtacaagc tgaaataccg atgataatta aaataaataa
     6961 tactagacaa ccaccacagc cccagaacca tcctttttta gatttctttt gtccgtattg
     7021 ctggttctgt tggttttgtt gattttggaa agcttggaat tgttcccatt gtcttcgctc
     7081 ttcttcgctt ttgaattgat tgctcatgcc taaaaaatct ccctcattat ttactatgtg
     7141 attagtgcat aaagctaaat acaatttgaa ttcaattcgc ttgttgttta atatttatca
     7201 ggttcacatg cacgtttatc tttatctcta tctaaatgtg gtcgatatgc tggatgatcg
     7261 gcagtgacac cattcggata tacttgtcta agttgcttac aattagcaaa tgattgttgc
     7321 gtgtttgttt cagttgatgg tgaggtctta ggttgcgtgg attgatgatg gttacttgat
     7381 tgttgctttt cttttaaatc atctttcaag tcgtcttgtg atttatcttc tgcaacgttt
     7441 ggtgtgttaa tatcatcgtc tgattgatct tttgttgtta tttcatcaat ttcatgttga
     7501 ttattttcag tattttcatt tgcaggtact tcttcggttg attgtaaatc ctcatctgtt
     7561 gctgtttctg atttttcctc aggcttatct tcatttttat tttcatcttt ttgagttaat
     7621 tgcgtttttg atgattgatt attagtggaa ttgtcattat tactaaacat cattgaacag
     7681 gctgataata agattattaa taaaataaaa gaaataaggc aaccgccgca tccttttaac
     7741 atgccgcgtc tttcagaatt tttaacagat tggttttgat tcttgttgtt ctctgtattc
     7801 aaaggtaaat ctcctctata tttatatgtt attttcatga tttactacat aaaacctgga
     7861 tgcatttaag agggagttat taagtgtaaa acgctttcac tatatcacat aatcatattt
     7921 taccaaat
//
LOCUS       DALAGF010000122         7628 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.122, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000122 DALAGF010000000
VERSION     DALAGF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.122"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            109..312
                     /locus_tag="O2692_002422"
     CDS             109..312
                     /locus_tag="O2692_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCZ4300026.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            complement(309..545)
                     /locus_tag="O2692_002423"
     CDS             complement(309..545)
                     /locus_tag="O2692_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300027.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            770..1351
                     /locus_tag="O2692_002424"
     CDS             770..1351
                     /locus_tag="O2692_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCZ4300028.1"
                     /translation="MAKTLYIMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGIFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEERIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1424..2041)
                     /locus_tag="O2692_002425"
     CDS             complement(1424..2041)
                     /locus_tag="O2692_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HCZ4300029.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGETQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF"
     gene            complement(2196..2690)
                     /locus_tag="O2692_002426"
     CDS             complement(2196..2690)
                     /locus_tag="O2692_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4300030.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
     gene            complement(3089..3511)
                     /locus_tag="O2692_002427"
     CDS             complement(3089..3511)
                     /locus_tag="O2692_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCZ4300031.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            3659..4375
                     /locus_tag="O2692_002428"
     CDS             3659..4375
                     /locus_tag="O2692_002428"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCZ4300032.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDSYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     VPQAPGQIDVTDLKAQVTLY"
     gene            4458..4997
                     /locus_tag="O2692_002429"
     CDS             4458..4997
                     /locus_tag="O2692_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HCZ4300033.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEGDMPKAPRKNRNLITLY"
     gene            complement(5147..5467)
                     /locus_tag="O2692_002430"
     CDS             complement(5147..5467)
                     /locus_tag="O2692_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCZ4300034.1"
                     /translation="MQSIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            5611..5967
                     /locus_tag="O2692_002431"
     CDS             5611..5967
                     /locus_tag="O2692_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCZ4300035.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            6125..6505
                     /gene="gcvH"
                     /locus_tag="O2692_002432"
     CDS             6125..6505
                     /gene="gcvH"
                     /locus_tag="O2692_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCZ4300036.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVAIIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQIEALLTAEKYSEMIGE"
     gene            6684..7562
                     /locus_tag="O2692_002433"
     CDS             6684..7562
                     /locus_tag="O2692_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HCZ4300037.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLNQSKIGGMPFLKYFKDIPLDENNVPMVLLAQINLDDLPEQQELFPVKEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
BASE COUNT         2755 a         1071 c         1287 g         2515 t
ORIGIN      
        1 tcaaaaaaat gtgcaaaatc acttttgcac atttacaaat actagttttc tgataagatt
       61 aataataaca ctttaaatag cgcttaataa atgaaggggg caagtcttat gacgttttac
      121 aatttcatca tgggttttca aaatgataac acaccatttg gtatattggc cgaacacgtt
      181 agtgaagata aagcattccc tcgattagaa gaaagacacc aagtaattag agcatatgtg
      241 atgtctaatt acacagatca tcaattaatt gaaactacaa atagagctat tagcttatat
      301 atggcaaatt aatttgagta gtaccaatta tgatgtatta gtgcatccca aatatctttt
      361 gttttaaagt ttatttcatc atttcttatc gaaaatggtg taataatgtc tttatctaac
      421 caagtgttga taagttcatt tggtacacca tctaacaaca tttcactttt actaattata
      481 aaacattccc agtcaagtga aacattttgt ggattcacat aattacattg attatgatta
      541 tccataaaca ctcactcctt taaaattctg tactcttcat tgcgttttac cccgtcacat
      601 tatcttttaa actaaaatca tcattactta tgaaaaaaat gtacatcaaa agcaaaggtt
      661 ttcgctaccg aaaaagttta aataatggtt taatatattt ggtactcatt ttaataaaaa
      721 gagaatacat tttgagctat caatactttt tattgaagag gtgttattgt tggctaaaac
      781 gttatatata atgcgccacg gacaaacttt gtttaatttt aagggactaa ttcagggatt
      841 tggagattcg ccgctaacag aacttggaat tgctcaagct caaaaggcac gtagttatta
      901 tgaaactaag gggataaact tcgatttata tgcatcatca acgcaagaac gcgcaagtga
      961 cacacttgaa aatgttgcac ctaaccaatc gtatcaacgt tttaagggac tgaaggaatg
     1021 gcattttgga atatttgaag gtgagtcagt ctatctattt gataatctat acaagcctga
     1081 ggacttattc ggagatcgaa ttgttccttt caaaggagag gcaaggcaac aagttgaaga
     1141 acgcattgtg aaaactttac atgacattat gtctcaaaca aagaataatg cattagtcgt
     1201 gagtcatgga acaataatgg gagtattttt aagatattgc cttaaactag atgaagcatt
     1261 aaagcataat atcggtaatt gtaatatcct gaaatttgaa tatgacaatg gaacatttaa
     1321 atttgttgag ttaattgatc caaatttata ataaataaag cgttatacat agacacaagt
     1381 atgtcttttg tataacgctt ttaaaataca actacataac gctttagaat aataattgaa
     1441 taagtttttg taatatcatc agagcaacaa taataattat tatgcttgat attttattga
     1501 ttattgttaa taattttccg gttttatcaa ttgatccaac catttttcct aaaattgcga
     1561 gtaaaaagaa ccataaccat gaaacgctaa tacaagcgat tgtaaacgca attttattgc
     1621 tgccactata taatgcagca ctactaccaa ttactccaat tgtatctaaa atagcatgtg
     1681 gattgagtaa tgaaactgat aaagcaaaac ttacttgttt cattggagac ataatttgag
     1741 tttctccatc tgttgagggt ttatcatgcc aaatggtcca agccatatac atcaagaaaa
     1801 ttaaaccaac tatataaata attgcttgaa gtacaggtaa agacataata atgatagata
     1861 ctcctaccac tgcaataata ataagtaagc tgtctgacaa cccggctgta attattgcag
     1921 gcaatacata tctatatttt ggttgattag ctccttggtt aaaaataaat acattttgtg
     1981 cacctaaagg taaaattaga ccaatcgcta agataaatcc atgaataatt gcggttacca
     2041 ttaagttttg acacccctaa aacatagaat attctgaaaa aacttattgt tatcatatca
     2101 aataaaaaac aactactcaa tagtagagta gttgtatgaa aaatagaacc tatatgattt
     2161 gaatttatga gaaatgtaga atatataaaa ctgtattact tattaatcaa atcataaaaa
     2221 agccaatgtt catcgaaata ggtatttccg attttaattg catttttctc aaaaccaaga
     2281 atgtcgaatc ctagtgcact atagaaaact ttagcaccta tgttgtttga cacaattgat
     2341 gtaagtaaag attcgtaatt gttctgccgt gcataattaa taatgaaatt aatgagctcg
     2401 ctattaatcg atttatcgtt attagtaaca aagttgtatt taattaatga tttatgttct
     2461 tttccaacgt atcgaatttg ttctaaggca gctgttgcga taagtgtttc gttttcataa
     2521 cagccaaata caatacattt cggtgaacct tgatttaaaa tgtcatgtat taaggtgtca
     2581 gtcattgttt gactataatg tgaatcttga gtgaattctt cattgactgt cgaaacaagt
     2641 ctcttatact cattaaaatc attattcgtt aatgttttaa tttcaatcat tactgacctc
     2701 cttaatcatt attaagaaag attttgtact aaaaaaattt cttaattaca tattaaaatg
     2761 tttattaaaa ttttgcaatc actgatttat atgcacattc ttgtaaatgg attatcaatt
     2821 taattatatt tatttttaag ttcaggaaat tatggaattg gttattggtc gacactggac
     2881 ttttatttgt agacggtatg cgaagaagtc aagccagtat tggaaccggt cacatttatg
     2941 atgactaagg tacaatcatt ttcagttaaa tattgccaga aatttcaatc acgaatagaa
     3001 aaaataacca accaaaaatt tttggttggc aaatttgaat tttatgtgaa ttttattaat
     3061 aacgttgaat aatctcttta agttaatgct aatctacaac atttacattt aaatcgacat
     3121 taatatttcc ttgagtcgct tttgaatatg gacaaaattc atgagccatt tgtaaatatt
     3181 tttcagcttc ttcttgagat ataacatttt taattgtcgc atcaattgaa acacttaatt
     3241 taggactttc tgagtctgaa tcatcttcta gtctcactgt tagtgttact tctggatgag
     3301 catcacgcac tttgttttgc tttaaaatta ggtcgaaagc accgttgaag caagatgcat
     3361 aacctgctgc aaataattgt tcggggttag tagctttacc atctgcttga gcaggcggaa
     3421 cgatatcaat atctaatgct cgatcatcag tataaacatg tcctttacgt ccgccaacat
     3481 tcgtcgcttt agtttcataa tgtattgcca tacttataac ctcctaattt aaaatacatt
     3541 tatgctttac ccattcgaaa ataccttaat cattttcatt tatagcattg tttgattgaa
     3601 ggataaaaag ttgttgttta caataaaaat aatgagtatc tgaaatgagg gattcactat
     3661 gacacatgtg gaagtagtag cgactatcgc gccacaatta tctatcgaag aaactttaat
     3721 tcaaaaaatt aatcatcgta ttgatgcaat agacgtatta gaattacgaa ttgatcaaat
     3781 tgaaaatgtc acagttgatc aagtggcaga aatgattaca aagctgaagg ttatgcaaga
     3841 ttcattcaaa ttattagtta cgtatcgtac aaagttacaa ggtggctatg ggcaatttac
     3901 aaatgactcg tatcttaatt taatatcaga cttagcaaat atcaatggca tagatatgat
     3961 tgatatagaa tggcaagcag atattgacat tgaaaaacat caacgaatca ttacacattt
     4021 gcaacagtat aataaagagg tggttatatc acatcataat ttcgaaagta cgcctccatt
     4081 agatgaattg caatttatat tttttaaaat gcaaaaattc aacccagaat acgttaaatt
     4141 agcagtaatg ccacataata aaaatgatgt gttaaattta ttgcaggcaa tgtctacatt
     4201 ttcagatact atggactgca aagttgttgg tatttcaatg tctaaacttg gactaataag
     4261 tagaacggct caaggcgttt ttggtggtgc gttgacttat ggttgtatcg gagtaccaca
     4321 agctccagga cagattgatg ttactgattt aaaagcacaa gtgactttat actaataaaa
     4381 tgaattaggt tgcttttgtg taagtgctgt cttataattg agaagtatta tcattgttcg
     4441 tggaggagcg attttagatg gaattacaac aagcaatagc taatagaaga agtgtgaaaa
     4501 aatttaaaag agatatgcac atagatgacg cattgctata tcaagcaatt gagaaagctg
     4561 ctgatgctcc aaatcacgga atgagggaac catggagagt tgtgcatgtt ccgaaagaca
     4621 gattaggaga tatgagtaag gatatttcta aatttgcatt tcctaatgaa ttagataagc
     4681 aacaatgtca ttatgatgca gttacgaaac taggtggcat gttattgctt attttaaaaa
     4741 cagatccaag acaacgtcaa aatgatgaaa actactttgc atttggtgca tatgcacaaa
     4801 atcttatgtt gttactttat gaagcgggaa taggtacatg ttggaaatcg ccattatata
     4861 tctatgatcc taaagtaaga aaaacacttg gtataaagaa agatgaagtt cttgctggat
     4921 tcttatattt aacggattta gaaggagata tgcctaaagc accacgtaaa aatagaaact
     4981 taattacatt atattaatat gtataattat agaaacatta ataaaagctg agtcatgaat
     5041 tgatggacat ctatcgagtt agagatttaa tctaacttac tagagtcggt acaataacag
     5101 tctcagcttt ttattgtgca gtatatacac atttttattt tagtatttat ttaaaagttt
     5161 ctgctaaaaa tgattcaact tgttcaggtg acttagcatt tgctgaatga aggtgtgcaa
     5221 ttttatcgcc gtttttaaat actagcaagc tagggatacc cataacttca ttttcaacaa
     5281 ctacatcttc taattcatca cgattaacag tataccattg gtaatcatta tattgttcta
     5341 cgattgggtc aatccataaa tccatagcac gacagtctgg gcaccatcct gcctcaaatt
     5401 taacaattac aggtgtatcg ctattaatta cagatttaaa tgattcatta cttttgattg
     5461 attgcattgt aacaactcct ctagatagtt taataatttt tattatagct aaatttatat
     5521 cataataaaa aattttagct tcaaaatgaa agccttttgc tttggaaaat gatatattta
     5581 ttttaaatac ataaaggagg ttgcagtcgt atgattaaat tttaccaata taagaattgt
     5641 acaacttgta aaaaggcagc aaagttttta gatgaatatg gcgtaagtta tgaaccaatt
     5701 gatatcgttc aacatacacc tacaataaat gaatttaaaa caataattgc aaatacaggc
     5761 gtagaaatta ataaattgtt taatacacac ggcgcgaaat atcgtgagct tgatttgaaa
     5821 aataaattac aaactttatc agatgatgaa aagttagagt tgttatcatc tgatggtatg
     5881 ttagtaaagc gtcctctagc agtaatgggc gataagataa cattaggatt taaagaagat
     5941 caatataaag agacttggtt agcgtaagtg aaatgtaagc gtttactaaa tatctcgata
     6001 tttagattca ttacatgtaa aatgaaataa gctatacaat tgttaatttt tataaatata
     6061 gttgaatagc atctagcctt atggcatcat taatgatgta aagattaatt aggaggggat
     6121 tctcttggca gtaccaaatg aattgaaata ttcaaaagag catgaatggg ttaaagttga
     6181 aggtaatgta gcaataattg gaatcacaga atacgcacaa agcgagttag gtgatattgt
     6241 tttcgttgaa ttaccagaaa cagatgatga aattaatgaa ggggatacgt ttggtagcgt
     6301 agaatcagtt aaaactgtat cagaattata tgcaccaatc tctggtaaag tagttgaagt
     6361 caacgaagaa ctagaagata gtcccgaatt tgtaaatgaa tctccatacg aaaaagcatg
     6421 gatggtaaaa gtagaaatta gtgatgaaag tcagattgaa gctttattaa cagctgaaaa
     6481 atattcagaa atgattggtg aataatcacc gtgtaactcc ttaatctaag attgaggagt
     6541 ttatttttag tctgagaaaa taaatgatat gaaagaaaaa ttattaggta ctattatttg
     6601 gagtattgct acattttatt attcaagaat gatggaaata atgaatttag ctattttaaa
     6661 aataaaaatt gggggaagtt aatatgctaa acattcaaga cgttagtcat ctttctaaaa
     6721 aggagcaaaa agcatataac cgtttcgtag aatctgtaga aaacggtaat ttaccagtac
     6781 taccatgtat tgaaatggat ctaaaagaga tgcaagaaga aacattaaac cagagtaaga
     6841 ttggtggaat gccattttta aaatatttta aagatatacc attagatgaa aataatgtac
     6901 caatggtatt gctagcacag attaatttgg atgatcttcc agaacaacaa gaattatttc
     6961 ctgtaaaaga agggatattg cagttttgga ttagttcaga agatcaaatg tatggaatgt
     7021 ctgaaaattt aaagggaaac aatataaact caaggcttgt ttatataaaa gagccaatta
     7081 cagatttatc actcgaaaat attcaagcgc atttgaagtc attagatgct gataatgagg
     7141 atatcccgtt cagtggagca ttttctatag aatttagatt gtcgaaacaa actattacat
     7201 gtactgatta taagtacgat gaggacgtgc ttgcattgtg gaataaagtc aatccatcct
     7261 tcgcgctaaa atcaatgttt ggtggttatg atgaattgat ggaacctgtg tgtaacacat
     7321 ttactgctaa ggaaccattt aatcaacttg gtggttatcc atattttgac caaatagatc
     7381 caagaacgaa cgatcaagaa ctgaaaatgt atgatagagt cttactgcaa attgattcta
     7441 caagagatgg taattcttcg attatatggg gtgatttagg tattgccaat atcttagtga
     7501 aatctactga ccttgaggct atgaagtttg atgattacat gtattcatgg gattgcagct
     7561 aagagaatca taaaaatata ttactttaat aagtgtagta tctatagatc aatctaggga
     7621 gtgggaca
//
LOCUS       DALAGF010000123         7420 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.123, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000123 DALAGF010000000
VERSION     DALAGF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7420
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.123"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..136
                     /locus_tag="O2692_002434"
     CDS             <1..136
                     /locus_tag="O2692_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300038.1"
                     /translation="YIVFLIIIWLNIFLGNEIIHTLTVLITTLYIVNSRKGIKNDRVE
                     "
     gene            93..317
                     /locus_tag="O2692_002435"
     CDS             93..317
                     /locus_tag="O2692_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300039.1"
                     /translation="MLIQERGLKMTELNNIINSLQSLFESESGYKISKNSGVPYQTVQ
                     DLRNGKTKLEDARFRTIIKLYSYYVSLKEH"
     gene            complement(762..1478)
                     /locus_tag="O2692_002436"
     CDS             complement(762..1478)
                     /locus_tag="O2692_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HCZ4300040.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KDRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYNENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
     gene            complement(1586..2311)
                     /locus_tag="O2692_002437"
     CDS             complement(1586..2311)
                     /locus_tag="O2692_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HCZ4300041.1"
                     /translation="MNNNITKKIILSTTLLLLGTASTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLTLKELDFRIRQTLIKSKKLYNNSYNKGQIKITGADNNYTIDLSKRLPS
                     TDANRYVKKPQNAKIEVILEKSN"
     gene            complement(2406..3131)
                     /locus_tag="O2692_002438"
     CDS             complement(2406..3131)
                     /locus_tag="O2692_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HCZ4300042.1"
                     /translation="MKKNIMNKLVLSTALLLLETTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKL
                     TDTNRYVKNPRNAEIEVILEKSN"
     gene            3569..4570
                     /gene="argF"
                     /locus_tag="O2692_002439"
     CDS             3569..4570
                     /gene="argF"
                     /locus_tag="O2692_002439"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCZ4300043.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEIMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            4593..5525
                     /gene="arcC"
                     /locus_tag="O2692_002440"
     CDS             4593..5525
                     /gene="arcC"
                     /locus_tag="O2692_002440"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCZ4300044.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            5697..7253
                     /locus_tag="O2692_002441"
     CDS             5697..7253
                     /locus_tag="O2692_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCZ4300045.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
BASE COUNT         2494 a         1051 c         1294 g         2581 t
ORIGIN      
        1 ttatatcgta tttcttatta ttatatggct gaatatattt ttaggaaatg aaatcatcca
       61 tacactgact gttttaataa caacattgta tattgttaat tcaagaaagg ggattaaaaa
      121 tgacagagtt gaataatatt ataaactctc ttcaatcttt gtttgagtct gaatcaggct
      181 ataaaatttc gaaaaattca ggagttccat atcaaacagt acaagattta agaaatggga
      241 aaaccaaact agaagatgct agatttagaa cgattattaa actttatagt tactatgtct
      301 cattaaaaga acactaatca tgggacaata aagtattgct ataataattt aatcattaaa
      361 tgaaggaata gagcctaaga cataataaat caatgtctta ggctctacaa tattatattg
      421 gtagtagttg accgaatgaa aatgcccatg taacaagcat ttttcaatta taggcggggc
      481 cccaacatag agaatttcaa aaagaaattc tacagacaat gcaagttggg gtgggcccca
      541 acacagaagc tgacgaaaag tcagcttaca ataatgtgca agttggggag tgacaacgaa
      601 ataaatttta taaaatacta tttctgtcac attcctactc ccaattccat ttaaatatat
      661 agaaaatttt acctattata tacatcatac actttaaaaa ttatcattta aataatctga
      721 tttggaatta aagtgcatga agtataagtc accttcatat actaatcaaa gaggacgtca
      781 acagttattt tattaggatt tttaacataa acatttgcta gatctgaatg taatcttttg
      841 cttaaatcaa tagtgtagtt attaccgcca ccggtgatct taagcttacc tttattacga
      901 ttttcgttat ataatatttt attttttatt aacgcttctc gtgcacggaa atcgatttct
      961 ttcaatgtta atactggttt attgccttgg tatattttat gcgcaccaat aatcgtttgt
     1021 agtttatctt tgtattgcac aaaaagatta taagttttat cagaaggttt tgcggctggt
     1081 gtaacgccac ctgtaaatgt ctctctataa gaccaccata actgatcagt atctttgtct
     1141 tttagtccaa acacatctac gtaacgatct tttaactgat taatatttcc ccaactttca
     1201 gcgccccata aagatatatg tgctgaccat gaatatctct taagttcaac ataaatgttt
     1261 ccattatcat attgatatag ccatttattt gaaaatgaaa aatgaggctg tgtgtaatat
     1321 ttaattaatt cattgatgtt agtttcatct tgaccaatgc tataagcttt agcttcagag
     1381 taaaaactaa atactggttt ttgattttga ggtaatacag tacctaatag taataatgtt
     1441 gtcgttaaaa ttatattttt cgtgatgttc ttactcatta gaacatctcc tttcagagga
     1501 atcatgatac gaggaataag aaatttaaat gtgagcgaag tcaatatagt atttgcgatt
     1561 atttttatta actccattat tattgttagt ttgatttttc gaggataact tcaatttttg
     1621 cattttgagg ttttttaaca tatctatttg catcagttga tggcaacctt ttacttaaat
     1681 ctattgtgta gttattgtct gcacctgtta ttttaatttg tcctttatta taagaattat
     1741 tatataattt tttactttta attaatgttt gacgaatacg aaaatctaat tcttttaaag
     1801 ttaaaacagg cttattgcct tcataaactg gaaatccgcc agtaaacgtt tctgctttat
     1861 ctttatatgt tacattcagt ttatagtgtt tatcgttaga tgttgctgca ggagtaacac
     1921 caccagtaaa cgtttcttga gataatgcaa aagaatcaat ggtttcttgg tcttttatgc
     1981 caaaaatatc aacgctttta tttcttaatt ggttgatatt gccccaactt tcaggtccat
     2041 aaacttgaat atgactatac caagaaaact gtaacaacgt tgcatgaatc gtaccgttat
     2101 ctttttgcca taacgtactg ttagagaagg ttaaatattt ttgcgagtaa tatttagtta
     2161 actcattaac gttagtttcg ttttgattta tataataagc tttcgcttca gatgaagaat
     2221 tgataggtgt attaggaaat tgtgtagatg ctgtacctaa tagtaacaat gttgttgata
     2281 aaataatttt tttcgtgatg ttattgttca ttcgaatttc tcctttgagt attgttggaa
     2341 tgtttaatta taaaaagggg ttaattagat aattgaaatt atccgcattt acaaaaggta
     2401 ataggttagt tagatttttc gagtatgact tcaatttctg cattacgagg atttttaaca
     2461 taacggtttg tgtcagttaa ttttaacttt ttacttaaat caatcgtgta attatttcca
     2521 tcagcagtta tcttaatttg acctttatta aattctccgt tatataactt tttatttttt
     2581 attaatgttt gacgaatacg gaaatctaat tcttttaaag ttaatactgg tttatttcct
     2641 ttgtaaaatt catgtccacc gatgatagtt tgttgtttat cactatattt taaaaatagt
     2701 ctataaggct tatcagatga agtagctgct ggcgtaacac caccagtaaa tgtttcatca
     2761 taagtccagt aaccttcaac tgtgtcctca tctttagttc caaatatatc aacgtattta
     2821 tttcttaact gattaatgtt tccccaactc tcggatccaa acacttgaat atgactatac
     2881 caaacccacg tttgcaatgt tgcatgaatg ctaccattgg gcttttgcca taaccatttt
     2941 ccagataatg aaaaatgcgg ctgagtgtaa tatttgatta gttcattgat attagtctcg
     3001 ttttcactga tattataggc ttttgcttca gatgaaaaac tgattggtgt tttaggaagt
     3061 tgtgttgatg tagtttctaa aagtaacaat gctgttgata aaactaattt attcatgatg
     3121 ttctttttca tatgaaaatc tcctttgcgt gaattaccca aagtatataa gctattacac
     3181 cgattcggaa ttaaataaaa gctaaaacta tgttaaataa acttaaacag ttagtagtgt
     3241 tatttaagca aaacttatca tttttaagtt ttggacagaa acagtatcta ataaagtagg
     3301 cgggagttat atattaaaaa cgatacgtta gaattattct ctaatcaatt gtattaaatt
     3361 gtttgataat tgaattttct aactacttga aaaatagtta tactttaaat gtagtactta
     3421 ttttaattat ttcctactac ttaaatttaa tattaataaa atgttcattt aattattgat
     3481 aaaatattac aaattttaat agtaggttgt gtttattttg tatgcgctta caatttaggt
     3541 gtaactaaaa taaaaggagt tgttattaat gaaaaattta cgaaacagaa gttttttaac
     3601 tttattagac ttttcacgac aagaggtaga attcttatta acactctccg aggatttaaa
     3661 acgtgctaaa tatattggca ctgaaaagcc tatgttaaaa aataaaaata ttgcactgtt
     3721 atttgaaaaa gattctacaa gaacgcgatg tgcatttgaa gttgcagcgc atgatcaagg
     3781 tgcaaatgta acttatttag gcccaactgg atcacaaatg ggtaaaaaag aaacaactaa
     3841 agatactgca cgtgtgcttg gtggaatgta tgatggcatt gaataccgtg gtttttcaca
     3901 aagaacagta gaaactttag ctgagtattc aggcgtacca gtgtggaatg gtttaactga
     3961 tgaagatcat cctactcaag ttcttgctga tttcttaaca gcaaaagaag tcttaaaaaa
     4021 agattatgca gatattaact ttacatatgt tggagatggt cgtaataacg ttgcaaatgc
     4081 attaatgcaa ggtgctgcca ttatgggtat gaacttccat ttagtttgtc caaaagaatt
     4141 aaatccaaca gatgaattat taaatcgctg taaaaatatt gccgctgaaa atggtggcaa
     4201 catattaatc acagatgata ttgaccaagg tgtaaaaggt tcggatgtaa tttacactga
     4261 tgtttgggta tcaatgggtg aacctgatga agtatggaaa gaacgacttg aattattgaa
     4321 accatatcaa gtaaataaag aaataatgga taaaactggt aatccaaatg ttatttttga
     4381 gcattgctta ccatctttcc ataatgctga tacgaaaata ggtcaacaaa tttttgaaaa
     4441 atatggtatt cgagaaatgg aagttacaga tgaagtattc gaaagtaaag cttcagttgt
     4501 attccaagaa gctgagaaca gaatgcatac aatcaaagca gtcatggttg ctacattggg
     4561 tgaattttaa atgatataag gaagtgaata tgatggcgaa aatagtagta gcattaggtg
     4621 gtaatgcttt aggaaaatca cctcaagaac aactcgagct tgttaaaaat actgcgaaat
     4681 cattagtagg attaataaca aaaggacatg agattgttat tagtcatggt aatggaccac
     4741 aggttggaag cattaatttg ggacttaact atgctgcaga acataaccaa ggtccggcat
     4801 ttccatttgc tgaatgtggc gcaatgagtc aagcttacat cggctatcaa ttacaagaaa
     4861 gcttacaaaa tgaattgcat tctattggaa tggataaaca agtggtaaca ctagtgacac
     4921 aagttgaagt tgatgaaaat gatccggcat ttaacaatcc ttcaaaacca attgggttat
     4981 tttacaacaa agaagaagct gaacaaattc aaaaagaaaa aggatttata tttgttgaag
     5041 atgctggaag aggatataga cgcgttgttc cttcaccaca acccatctct attattgaat
     5101 tagagagtat taaaacactt attaaaaatg atacactcgt tattgctgct ggtggtggag
     5161 gtataccagt aattagagag caacatgatg gttttaaagg tattgatgca gttatagaca
     5221 aagataaaac aagtgcgttg ttgggtgcta atattcaatg cgatcaattg attattttaa
     5281 cagcaattga ttatgtatat attaatttta acactgaaaa ccaacagcct ttgaaaacaa
     5341 caaatgttga tgaattaaaa cgatatatag acgaaaatca atttgcaaaa ggaagtatgt
     5401 taccaaaaat tgaagcagcc atatcattta ttgaaaacaa tccaaaagga agtgtgctta
     5461 taacatcatt aaatgaatta gatgctgcct tagagggtaa agtaggtact gtgattaaaa
     5521 agtaattgaa ttgaaacgct tttcaattac tatatgtcaa atgcatgatt tttattattt
     5581 atgtgcaccc cctgaaaata atgcctctat tttgatgcgg ggtgcacttt cttaatttat
     5641 atttataaaa tctttaaggt agaaatgcta ggttaaatgt cgaaggagat gaaaccgtgg
     5701 aaaatacaat taatgaaagt gaaaagaaaa aacgatttaa attaaaaatg ccaggtgcat
     5761 ttatgatttt attcatttta acggttgttg cagttatagc aacatgggtt attcctgctg
     5821 gtgcatattc taaactttct tacgaacctt catcccaaga actaaagata gttaaccctc
     5881 ataaccaagt gaaaaaggtt ccgggtacgc aacaggaact agacaaaatg ggggttaaaa
     5941 ttaagattga acaatttaaa tcaggtgcaa ttaataagcc ggtatcaatt ccgaatactt
     6001 atgaaagatt aaagcaacat ccagctggac cagaacaaat aacaagtagc atggttgaag
     6061 gtacgataga agcggtcgat atcatggtat tcattcttgt actaggggga cttattggcg
     6121 tagttcaagc cagtggttct tttgaatcgg gattgttagc tttaacgaag aaaacaaaag
     6181 ggcatgaatt tatgctaatt gtgtttgtat caatactaat gattatcggc gggacattat
     6241 gtggtattga agaagaagct gtagcatttt atccgatttt agtccctata tttatagcgt
     6301 taggatacga ttctatcgtt tcagttggtg ccatattcct tgccagctct gtcggtagta
     6361 cattttcaac tattaacccg ttctcggttg taattgcctc taatgccgct ggtacaactt
     6421 ttacggatgg cttgtattgg agaataggtg cttgtattgt cggtgcgatt tttgttatta
     6481 gttatttata ttggtactgt aaaaaaatta aaaacgatcc taaagcgtca tattcttatg
     6541 aagacaaaga tgcttttgaa cagcaatggt ctgtattaaa agatgacgat agtgcccatt
     6601 ttactttgcg taagaagata atccttacat tatttgtact accatttcca attatggtat
     6661 ggggagttat gacgcaaggt tggtggttcc cagttatggc ttcagcattt ttaatattta
     6721 caattataat aatgtttatt gctgggacag gtaaatctgg attgggagaa aaaggaactg
     6781 tagatgcatt tgtcaatggt gcatcaagtt tagtaggtgt atctttaatt attggtttag
     6841 ctcgaggtat taatttagtg ttgaatgaag gtatgatttc agatacaatc ttacactttt
     6901 catcatcttt agttcaacat atgagtggac cattatttat catcgtatta ctatttattt
     6961 tcttctgttt aggttttatc gtgccatcat cttctggctt agcggtatta tcaatgccta
     7021 tctttgcacc actagctgat acagtaggta taccaagatt cgtcatcgtt acgacatatc
     7081 aatttggtca atatgcgatg ttattcttag cgccgactgg acttgttatg gccacactac
     7141 aaatgttaaa catgcgatat tcacattggt tccgatttgt atggccggta gttgcttttg
     7201 tattgatttt cggtggcgga gtactaatta cgcaagtact aatttattca taatttgaaa
     7261 tgctatatta taaaaaatac taatggggtt ttatgcatct cgtaggtttg tagaaatact
     7321 aaactaagcg aggtgcatta ttatttttga ttaagaaaat aatgacggta atgataacac
     7381 tagtaagtaa ttgatacaat gctctattta ataatgatat
//
LOCUS       DALAGF010000124         7302 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.124, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000124 DALAGF010000000
VERSION     DALAGF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.124"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            372..1433
                     /locus_tag="O2692_002442"
     CDS             372..1433
                     /locus_tag="O2692_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCZ4300046.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFEVPKQ"
     gene            1691..3148
                     /locus_tag="O2692_002443"
     CDS             1691..3148
                     /locus_tag="O2692_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCZ4300047.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            3141..3863
                     /locus_tag="O2692_002444"
     CDS             3141..3863
                     /locus_tag="O2692_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCZ4300048.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            4014..5141
                     /gene="queG"
                     /locus_tag="O2692_002445"
     CDS             4014..5141
                     /gene="queG"
                     /locus_tag="O2692_002445"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCZ4300049.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPLIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            5146..5616
                     /gene="trmL"
                     /locus_tag="O2692_002446"
     CDS             5146..5616
                     /gene="trmL"
                     /locus_tag="O2692_002446"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCZ4300050.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            5775..6374
                     /locus_tag="O2692_002447"
     CDS             5775..6374
                     /locus_tag="O2692_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HCZ4300051.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            6399..6551
                     /locus_tag="O2692_002448"
     CDS             6399..6551
                     /locus_tag="O2692_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300052.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
BASE COUNT         2575 a          957 c         1351 g         2419 t
ORIGIN      
        1 tttaacttct ttttttggta ttacattaat gataatgatt atattatctg gggttatata
       61 tttgtttcaa cttttctaat aatatatttg taaaagtgct attttattgt gctttatacc
      121 aagtcgattc aatttatcta tcttaatagt aatatggcta ttttaatatt tacaatttat
      181 tacattatta gtcacaggat gtaattagta gtattttaaa attaacatga cattgattag
      241 tgagtagatt gtttaaattt gctttattca atttgtggat tgttatattt agattaaatt
      301 agccaactaa tcattgttaa agttaatttt ctataatatg atgagtttaa tgttcaaatt
      361 ggaggtcgtt tatgagtaac gcaaataatg ctgataataa acagtttttc agtaagattt
      421 taaattctat tggtgctggt gttgttattg cacttgttcc gaatgcttta ttaggtgaag
      481 tattaaaaat atttaaaagt ggtaatgaaa ttttagaatt aacgtatcag ttagtgatac
      541 taattcaatc atttatggca tttattattg gggttttagc tgctcatcaa tttaaattta
      601 atggtgcagg tgctgctata gtaggtacat cagctatgat tggttcaggt gctgttgtat
      661 atagcaataa tagttttatg ttaaaaggta taggtgacat tataaatacg agtttggttg
      721 ttattattgc atgtcttata tatatggtat tacaaaacaa acttggatct tttgaattaa
      781 ttatattacc agttttagta cctatcgtta gtgggggtat cggtctaatc acattacctt
      841 acattcgaaa aattactcaa gcaattggaa atgtaattca ttcttttaca gacttaaatc
      901 cgttgttaat gtctatactt attagtgtgg cattttcatt attaatggta acaccaatat
      961 cgttagtagc gattgcaact gcgattagtt taaacggact aggaagtgga gcagcaaacc
     1021 taggtatagt ggctgcctgt gttactttct tatttggctc attgcgtgtc aattctattg
     1081 gtgtaaatgc agttttactc ataggagctg caaaaatgat gatcccagtt tacttgaaaa
     1141 atttaattat ttcgatacca ttgactataa atggaataat tactggaatc attgcttacg
     1201 ttcttcaggt taaaggaaca ccattgtctg ctggatttgg atatacagga ttagttggcc
     1261 caataaatgc atttaaccga atgtcaggtg atccaacaat gaatattatt ttattagcac
     1321 ttggttattt tgtcatacca tttgtatcgg catttattgt tcatgaattg tgtaaaaaat
     1381 tcattcctat atattctaat gatatttata aatttgaagt tccaaaacaa taatttatag
     1441 cttgatgaca agaataagtt ttaatatatg ataaatatag gcgttactgt caataagata
     1501 ttgatagtag cgcttttttg attgatttta aaatgtgaga tttgttcacg tataatagcg
     1561 gattttttat gtacagtctt ttaaaaagaa gcttttcttt atattgataa aaatacattt
     1621 ataaaacact attgcatcaa aatgtattaa tatgtataat tatgaatatt aattagggag
     1681 ggctgtataa atgaagtgtt taattaggtt tatattagta ttagggttgt taattagcag
     1741 tgctatggtg tatatcaatc ccacagcaca tgctgaacaa gatcaaacat gggagaagat
     1801 taaagaacgc ggtgaactta gagtgggtct ttctgcagat tatgcaccaa tggaatttga
     1861 gcatacagtt aatggtaaga ctgagtatgc aggtgtagat attgatttag ctaaaaaaat
     1921 tgcgaaagat aataatttaa aattaaaaat cgtcaatatg tcatttgata gtttgttagg
     1981 agctcttaaa actggaaaaa ttgatattat tatttccgga atgacttcaa cgcctgaacg
     2041 taagaagcaa gttgattttt cagattcata tatgatgact aaaaatatca tgcttgtaaa
     2101 gaaagataaa gttaatgaat ataaagatat caaagacttt aataataaaa aagtaggggc
     2161 acaaaaggga actgaacaag aaaaaatcgc tcaaaccgaa attgaaaatg catctattac
     2221 ttcattaagc cgtttaccag acgttatatt agcattgaaa agtggaaagg ttgaaggtgc
     2281 tgtagttgaa aaacctgtag cagaagcata tttaaaacaa aatcctaaat taggaatttc
     2341 caatgtgaaa tttaatgaag aagaaaaaga tacagtgata gcagtgccaa aagattcacc
     2401 aaaattattg tcacaaatta ataaaacgat taaggaggtt aaagataaag gattaatcga
     2461 taaatatatg actaatgctg caaatgcgat gaatgatgac agtggtttta tttctaagta
     2521 tggtagtttt ttcttgaaag gaattaagat tacaatatta atttcactta tcggtgttgc
     2581 attaggttct attttaggtg cattcgttgc gttaatgaaa ttaagtaaaa taaaaattat
     2641 ttcatggatt gcttctatct atatcgaaat attaagagga acaccaatgt tagttcaagt
     2701 atttatcgta ttctttggta taactgctgc attaggacta gacatttcag ctcttgtttg
     2761 tggaacaatt gcattagtta ttaattcctc agcttacatt gctgaaatta ttcgtgcagg
     2821 tataaatgct gttgataaag gccaaatgga agccgcacgt agtctaggtt tgaattatag
     2881 acaaacgatg aaaagtgtaa ttatgccaca agcaattaaa aatattttac cagctttagg
     2941 taatgaattt gtcactttaa ttaaagaatc atctattgtg tcaacaattg gagttggcga
     3001 aattatgttt aatgcacaag tggttcaagg tatttcattt gacccattca caccattaat
     3061 agtggcagca gcattatact ttgtcttaac atttgtactt acacgcatta tgaacatgat
     3121 tgaagggaga ttgaatgcca gtgattaaaa taaacaatct taataaagtt tttggagata
     3181 atgaagtttt aaaagatatc aatcttgaaa tcaatcaagg ggaagtagta gcaataatag
     3241 gtccatctgg tagtggtaaa agtacattgt taagatgtat gaatttatta gaagtaccta
     3301 ctaaaggtca agtgattttt gaaggcaatg acttaacgga aaaagggaca caagtagata
     3361 aactacgtca aaaaatgggt atggtatttc aaaacttcaa cctatttcca cataaaaaag
     3421 ttgtcgataa tattatttta gctcctaaat tattaaagaa agataataac gatgaattac
     3481 ataaggaagc attgtcgtta ttagataaag tgggattaaa agaaaaagca gatgtatatc
     3541 cgaatcaatt atcaggtggt caaaaacaaa gggtagcaat tgcaagagct ttagcaatgc
     3601 atccagatgt tattttattc gatgaaccaa cttcagcatt agatcctgag gtagttggtg
     3661 atgtattaaa agtaatgaaa gacctagcca aagaaggtat gaccatggtg gttgtgacac
     3721 atgaaatggg atttgccaaa gatgtaagtg acaaagtcat atttatggca gatggcgttg
     3781 tcgtagagtc aggcacacca gtcgaaatat ttgaacaacc gcaacatgaa agaacacaaa
     3841 atttcttagc aagagtatta taacaaccta acgaagcttg aatatatgat acgcaccaca
     3901 aagttatatc atatattcaa gctttttatt attaaaatta tgtgaaatga aacctcaata
     3961 tgaataatgg ttgtgttaaa ataacatagt attattttat ggggggcata cgattggata
     4021 caaagcaatt aaagcaagat atcattgact atgcatatac aattggtatc gacagtattg
     4081 gatttactac tgccgatccc tttgatgaat tgaagcaaaa gctagaagca tatcatgcaa
     4141 atggttatgc ctcaggattt gaagaatctg atattgcttt acgaacggag cctaaattat
     4201 ccttaccaac agcaaggtca atcatagcaa ttgcagttgg ttatcctaac aaactgaaag
     4261 gtgcacctaa gagtgttaga ggtgatcgca gaggcttatt tgctagagca tcgtggggtc
     4321 aagattatca tacaattatg cgtaaacgat tagacatgtt agctgcattt attgaatcta
     4381 aagttccaga tgttgaaatc aaatctatgg tagatacggg tgtattatca gatagggcag
     4441 tagcagaacg tgcaggttta ggatttgttg gtcgtaatgg ctttgtcatc aatcctaaac
     4501 taggaacatg gacatacctc ggtgaaatgt tagtcagtat accttttgaa cctgatgatc
     4561 cattattaga tagctgtggt gattgtacaa tttgtgttga tcgttgtcca acaagtgcat
     4621 tagttggaaa tggccaatta aatagtcaga aatgcattag ttttttaacg caaacaaaag
     4681 gctatatgcc tgaccaatat cgttataaaa ttgggaatag attatatggt tgcgatacgt
     4741 gtcaacaagt ttgtccgaaa aatagaggta ttaataccga acaagatgac atcattttgg
     4801 aaccagaaat tttaaagcca agattagtac ctttactacg tatgtctaat aaagaattca
     4861 aacaaacata tggtcacctt gcaggtgctt ggcgtggtaa aaagccgata caacgaaatg
     4921 ctattttagc attggcgcat tttaatgaag tggatgcaat tccagaattg aaaaaagttg
     4981 caacaacaga tgaaagaccg ttgattcgag caactgctta ttgggccatt ggtcaaattc
     5041 ttggggaaga agcaagagat tttatcaatg ctaattatga tcaagaggat gcagaagttc
     5101 agaatgaaat gataaaagga ttagacacaa ggagagaata gtaatatgac aaatcatatc
     5161 gttttatatc aaccagaaat cccagctaat actggtaaca ttgcacgtac gtgtgcaggt
     5221 acaaacacac atttacattt aattaaacct cttggattta gaactgatga taaaatgtta
     5281 aaaagagccg gtttagatta ttgggaattt gtgaatatta cgtatcatga tagtattgaa
     5341 gcattttttg aagtcacaaa tggcgagtat tacttattaa caaaatttgg taaaaagact
     5401 tatagtgatt ttgatttttc aaatcatgac aaagattttt acttcatttt tggaaaagaa
     5461 acgacaggat taccagactg ggtgaaagag aaatatcaag acacagcgtt aagaattcct
     5521 atgagtgaac atattcgttc attaaattta tcaaatactg ctgcgttgtt aatttatgaa
     5581 gcgttacgtc aacaagattt tccgggattg aattaaaatt tactcatttt cattataaaa
     5641 atgatgctta agtgctatat ttagagtata agcatgttaa agttaaatgg tttgatatat
     5701 tgaaagttag ggtataataa ttattaatat cgccgttttt atgtaaaatg caaactaaga
     5761 ggagtgtaga aatcatggca atgaacttta aagtctttga caatagtcaa cttgtagcag
     5821 aatatgctgc tgatattatt agaaagcaat ttaacaataa tcctactaca attgcaggtt
     5881 ttcatttaga tacagatcaa gcgccagttc tagatgaatt aaagaaaaat gttgaaaaac
     5941 atgctgttga ttttagccaa ataaatattt tagattatga cgataaaaaa tcatatttcg
     6001 aagcgttagg tgtaccagca ggtcaagttt atccaattgc ttatgaaaaa gatgcaatcg
     6061 aattaatcgc tgataagatt aaaactaaag aaaataaagg gaaattaaca ttacaagttg
     6121 tttctatcga tgagcaaggt aagttaaatg ttagtattcg tcaaggacta atggaagcaa
     6181 gagaaatttt cttagtagtg acaggtgcta ataaacgaga tgtagttgaa aaattatatc
     6241 aagaaaatgg taaaacaagc ttcgaaccag ccgatttaaa agcacataga atggtaaatg
     6301 ttattcttga taaagaagcg gctgcaggtt tacctgaaga tgttaaagct tactttacgt
     6361 cacgctttgc ttaattaaaa atctgtgagg tattgattat gtctaaagac aaagatccaa
     6421 aattaaatta tcatgaagaa gaaaacagta tggtaacgga ttttgaagat ttaaaagaat
     6481 taggtaaaga aatggaacaa atctctgatc aaaatgatca agaaaagaat tctgaagaag
     6541 acagtcagta aagtattata attgtgtaat attcagttat ttatcatgta aataatgtat
     6601 agtatagaac atatatcaaa taggattgat tatcttgaat atgaagttag gtagcatttt
     6661 gttacctagc ttttttatta tattcataga agctgacttt ctgctagttt cagtgttggg
     6721 gctgtttccc caacttacat tgtttgtaga aattgaggat ccaatttttc tatgttgtgg
     6781 ccccgccaac ttgcatgtta ttttaagctg actttccacc aacttctgag tttgtggccc
     6841 gtttccctaa tttgcaatgt ctgtagaaat tagaaatccg atctcactat tttggggcct
     6901 cgccaacttg catgttattt taagctgact ttccaccagt ttctgagttt gtggcccgtt
     6961 tccccaattt gcaatgtctg tagaaattgg gaatccaatt ccactatgtt ggagctccac
     7021 caacttgcat gaaatagaag ctgacattcc gaagcctcta tgttgtggac tgcagtcaac
     7081 tactgccaat ataactttgt aaagcataaa acattgattt gtgtaccaga catagtaata
     7141 tatcaaaagt tcaattttaa atattaaaac accatttaaa cggaattgat aattaattac
     7201 tccagacata attgaaagaa gtcatctatc agtaatattt ttaatgaaat cattaaaccg
     7261 cgtttcttaa tatgccaaga ttatgatatg ctattgaaga at
//
LOCUS       DALAGF010000125         7285 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.125, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000125 DALAGF010000000
VERSION     DALAGF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.125"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            8..1201
                     /gene="fmtA"
                     /locus_tag="O2692_002449"
     CDS             8..1201
                     /gene="fmtA"
                     /locus_tag="O2692_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HCZ4300053.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKKYPDEYRYGFY
                     AKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
     gene            complement(1738..2016)
                     /gene="qoxD"
                     /locus_tag="O2692_002450"
     CDS             complement(1738..2016)
                     /gene="qoxD"
                     /locus_tag="O2692_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HCZ4300054.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(2025..2630)
                     /gene="qoxC"
                     /locus_tag="O2692_002451"
     CDS             complement(2025..2630)
                     /gene="qoxC"
                     /locus_tag="O2692_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HCZ4300055.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(2620..4608)
                     /gene="qoxB"
                     /locus_tag="O2692_002452"
     CDS             complement(2620..4608)
                     /gene="qoxB"
                     /locus_tag="O2692_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HCZ4300056.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(4608..5708)
                     /gene="qoxA"
                     /locus_tag="O2692_002453"
     CDS             complement(4608..5708)
                     /gene="qoxA"
                     /locus_tag="O2692_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HCZ4300057.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            6010..6132
                     /locus_tag="O2692_002454"
     CDS             6010..6132
                     /locus_tag="O2692_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300058.1"
                     /translation="MIERKRKHFNKEFAKSNLSLNNEKWKFVCSKSKHFFKKSS"
     gene            complement(6280..6597)
                     /locus_tag="O2692_002455"
     CDS             complement(6280..6597)
                     /locus_tag="O2692_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HCZ4300059.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
BASE COUNT         2584 a         1142 c         1232 g         2327 t
ORIGIN      
        1 ttatcaaatg aaatttaata aagtaaaact agttatacat gcgtgtgtac tattatttat
       61 cattatttct atagcgttaa tttttcatcg attacagacg aagacacatt ctatagaccc
      121 aatacataag gaaacaaaat tatcagacaa tgaaaaatat ttagtggatc gtaataagga
      181 aaaggttgcg ccgtctaaac taaaagaggt atataatagc aaggatccta aatataagaa
      241 aattgacaag tatttacaaa gttcattatt taacggttca gtagctatat atgaaaatgg
      301 caaattgaaa atgagtaaag gttatggata tcaagatttt gaaaaaggta ttaaaaacac
      361 accgaatacg atgtttttaa taggttcagc tcaaaaattt tcaacagggt tactgttaaa
      421 acagttagaa gaagaacata aaataaatat caatgatcca gtaagtaaat accttccatg
      481 gtttaaaaca tctaagccta tcccattgaa agatttaatg ttgcatcaaa gtggattata
      541 taaatataaa tcctcaaaag attataaaaa tttagatcaa gcagttaaag cgattcaaaa
      601 acgtggtatt gatcctaaga aatacaaaaa gcatatgtat aacgatggga attatttagt
      661 acttgcgaaa gtaattgaag aagttacagg taaatcttat gctgagaatt attatacaaa
      721 aataggagat cctttaaaac ttcagcacac agcattttat gatgaacaac cttttaaaaa
      781 atatctagca aaaggttatg cttataatag tacaggactt tcattcttaa gacctaatat
      841 tttggaccaa tactatggtg caggtaattt atatatgaca ccaacagata tgggtaaatt
      901 aattactcaa atacaacaat ataaattatt cagtcctaaa ataaccaatc cattattaca
      961 tgagtttggt acgaaaaagt atccagatga atatcgatat ggtttctatg ctaagccaac
     1021 attgaataga cttaacgggg gattctttgg acaagtcttt actgtttact ataatgataa
     1081 gtatgtagtt gtacttgcat taaatgtaaa aggaaacaat gaagttcgaa tcaaacatat
     1141 ttataatgat attttaaaac aaaataaacc ttacaatacg aagggtgtta ttgttcaata
     1201 attaatatag aagatataac atgtatatgg cattaaggca tcgaccttat ctgaccagta
     1261 tacgagttat atcttctttt ttatagtggt aaaaagttta aagtataagg ttgaagaagg
     1321 atgagtttaa aaatatgtgt taactgataa aaggggaaat catttggtga gttggcatca
     1381 gactaaaatg aatgaagacg aatacgttgg tccatgcgtg gtgtgaatgt tttctaaatc
     1441 taactggaaa tgataattta ataatgtaca cttttgattg tttaaacatg tacaattttt
     1501 aaaaacctag ggataatttt attgacgtga acaacattga gattatcttt agacataaat
     1561 atggattttg aagttactat atttattagt tgtggttgtt aatgtattaa ttgaattgat
     1621 tataatgatt gtcttataag atatagaagg ggacttaacc atttaaaaat tgaaggaaaa
     1681 taaaaaccag ctaacaaaag tgttagctgg ttaaacttta cattattaaa atgttgttta
     1741 taagtgtgaa gagtgaccgc cttgcataac ccaatatgtt ccgacaacga aacaaagtgt
     1801 aattacaaga gcaaagataa ctttgaatgt ttgtaaacgt ccatctttac cttcagttaa
     1861 atgcatgaac attaataatt gaagtcctgc ttggacgaat gcaaagccaa agataattgt
     1921 caacttcgcg tggaatgtta atgacgtgta tagtgttacg taaactgcta aaagcgttaa
     1981 tacgatagat gcgataaatc ctacagtatg tttcattatt gtactcatcc gctatacacc
     2041 atccctatca tatatacggc agtaaagatg aaaacccaaa caacatctaa gaagtgccag
     2101 tataaactta ctataaataa ttttggcgca ttatatttgt ctaatccgcg tcgttggatt
     2161 tggattaata aacaaatggc ccaaacgata cctagcgata cgtgacaacc atgcgttcct
     2221 aataggataa agaaactaga ccagtaagaa ccaattgttg ggttaacgcc ttctgatgca
     2281 tagtgtgcga attcataaat ttcgaatcca acaaagacta aacctaaaag taacgtaatg
     2341 atcatccaaa acatcattaa cttttgtttt tcttggcgca tgtagtaaat agcaatacca
     2401 catgtgtaag aactgaataa taatgcaaac gtcattatta aaacaagagg caattcaaat
     2461 aactcagtag tcattttacc tgcataatcg ccaccatgtt gcaaagttaa tagtgtcgca
     2521 aatagggtac cgaataacgc aaattcggct gtaatgaaaa tccaaaagcc aagcttattt
     2581 aattcgcctt catgtgtgcg tgaatcaata gtgtttgtat catgactcat gacttacagc
     2641 ctccctttct ttaatacgcg cttctcttaa tctagcttca gtttctgcaa cttcagcagc
     2701 agggatatga tatccgtgat cgatttggaa actgcgataa atcatagtac caaaaatacc
     2761 gaataaacaa attaatgctg gaattacagt ttcgaaaatt aagaagaaac cgccgatagt
     2821 cataaagata ccaatccaga atccaacagg agtattgttt ggcatatgaa tgtctttgta
     2881 attatggttg tctaagtaat gacgaccatg ttctttcata tcaacaaatg tgtcgtagtc
     2941 attccaatct ggtgtaatgg caaagttgta tttaggtgga attgctgatg cagtagtcca
     3001 ctctagagta cgaccaaggc catcccagtt atctccagtt gcttcacgtg gagatttgaa
     3061 gtgactgtat acgatactaa caacaaggaa taagaatccg attgccatta ataatgcacc
     3121 gatagttgaa attaagttta ataagaacca accatctgat ggcatataag tgtataaacg
     3181 acgtggcata ccatctaatc caagaatgaa ttgtggtaag aaacaaacgt taaatccgat
     3241 catgaagaac cagaagcacc atttgtttaa tgtttcgttt aatttgtaac ccatcatctt
     3301 tggataccag aagattaaac cagctaagca ggcaaataca acaccagtaa ccaatgtata
     3361 gtggaagtga gctactaaga agtacgtatt gtgatattga tagtcagctg atgccattgc
     3421 taacattaca cccgtaacac cacctaaaag gaagttaggg ataaatgcta atgagaatag
     3481 cattggtgac tcaaatgtaa tacgtccttt atataatgtt aataaccagt taaacaattt
     3541 cacaccagtt ggaataccaa tcagcattgt tgaaattgag aagaatgagt tgattaacgc
     3601 accattaccc attgtgaaga aatggtgaac ccaaactaag aaactaagga acgcgatgcc
     3661 ggcagttgcc cataccatac tttgatgtcc gaataaacgc ttacgagcga atgtcgggat
     3721 aatttctgag taaataccaa atgctggaag gataacgata taaacttcag ggtgccccca
     3781 tacccagaag aagttagccc aaagcattgg cataccgcca tgtgcaactg tgaagaatgc
     3841 tgtgtcaaat attctatcag ttgtcattaa tgctaacgct actgttaaag gagggaaagc
     3901 aagaataaca attaatgtag taataaatgt tgttactgta aacattggca tttgcataaa
     3961 cttcatagtt ggtgttttac atcttaaaat tgttacaaag aagttgatac ctgtagctaa
     4021 ggtaccaagc cctgaaattt gtatagctat taagtaatag ttaacacccg gaccaggact
     4081 gaattcacct gctagtggcg catagtttgt ccaaccagct gctggtgaac caccaataat
     4141 aaatgacagg ttgaataaaa tcatacctgc aaagaatagc cagaaactta cgttgtttaa
     4201 tactgggaat gcaacatcac gtgctccaat ttgtaatgga acaacgatat tccataaacc
     4261 aaagataaat ggcattgcca tgaagataat catgattaca ccatgtgtac taaaaatttc
     4321 gttatagtgg ttagattcta aaaatttgtt atcaggtact gttaattgcg cacgaataag
     4381 taacgcatca ataccaccac ggacgaacat taatacggca cagattaaat acataatacc
     4441 gattttctta tggtctacag atgtgaacca ttctttgtaa agatatttcc ataatttaaa
     4501 gtaagtaatt actgcgatta aaccaataac taagaatggg gcaccaattt gtgccattgt
     4561 aatcatccag ttacctttaa ctagtaattg atcccatgga aaattcatta atgtccacct
     4621 ccatgatcat cattgtcttg atcttgcgca tcttttgaaa ttttcttcat ttctttcgca
     4681 tttttcgatt catctttctt gaactcattg ttatatggtt cgtcatttcc aagaatcatc
     4741 aacttcatac catgtcgttt atagttcgca tttgtaattt gagctttacg agcaggtatt
     4801 aatggtttgt ctgatacatc tttaaacata ttttcttcac tagtgaagtt tggatctttt
     4861 aattcgaaat tgaaacgttt atatgcatag aagatgtatt ctggatcggc tgctggatca
     4921 acaaacgcca tatgtgtacc attaaattct aaagctttat taggtgtgct tggtaataat
     4981 tgtttatcaa atgtatcttg atctaacgtt ttcttacctt taacttcttt cacccatttg
     5041 tcgtagtctt tttgactaac ggcatttact ttaaatgttt gacgtgagaa tccttcacca
     5101 ttgaagttag agttacgacc tctgaacgta ccagtttgag atgcttctaa cgtccaattc
     5161 attgtcatgc cagtcatggc atatttttga ccacctaatt gtggaatcca gaaacttgtc
     5221 attgtatcca tagcttgaag cttaaataca acaggacgat ctttagggat tgttaatgta
     5281 ttaacagtct ctatatgttc atctggataa gcaaagaacc atttgtatcc tgcacttact
     5341 gcatatacaa ccattggatc tttctcactc ttcggtggtt tttcgtaatc gtataaagtt
     5401 ttaactgtag gaatagctaa agcagcaacg attatgatag gtattacaaa ccatattgtt
     5461 tcaatgatgg cattatggtg catcttacca gattcggcat tcttattata actatacttg
     5521 taaataaaaa tggcgaacat gccaagtaca acgaaacaaa taacaagcat gaagacgatt
     5581 gaataaagaa tcaagaactt ctgactactt gctactggcc cttttgcgtt gaaaatttct
     5641 atatttgaac aaccactaag taaaattagt gtgccaaata atagaagcaa agacttaaat
     5701 tttgacactt ttttgacctc ctaatactac aaatgtaggg cttaacatta attttaagtt
     5761 attacacaat atttacaagg gcttatggga aaaaaattaa taaaattgta tcaaaaatgt
     5821 tgataaatca aggtgtgacg tgggttcaca catttgttaa aattatgtgt acattttgtg
     5881 actaatagcg tttttgaaaa aattgcttca tatttaaaag gaaagttgag aatagctttc
     5941 aattaaaggt taattgaatg aaaataaaaa aagtttgaaa ttgtctaata aattgaaaat
     6001 gatggctagt tgattgaaag gaagcgcaaa cattttaata aggaatttgc caaaagtaat
     6061 ttatcgttaa acaatgaaaa gtggaaattt gtttgttcaa aatcaaaaca tttttttaaa
     6121 aagagcagtt aagttttaat aatttgatag gtagtccttt ggtatacaga aaaaatacaa
     6181 tatttgggtt aaagttgcag atgaagttag aataacaaat aaaatggtag caaaacatgt
     6241 tcgtttgcta ccattttatt tgcagtatag tgatgagggc tatttaacct caatatacct
     6301 agtggaaatt ttaattgcac catctgaatc agtgacatga tatttaattt gatatttacc
     6361 agatttagat gtatcaattt ggccatcgac tttaatttta tcggttaaat ctccatcttc
     6421 tttatcaaat gcacttatgc cgtttagaag gttataatct tgacctttct taataacgat
     6481 atcattagcg cctttaattt gtggtgtagt attcgtcgtt gcatctgcat ttaaatttgg
     6541 tgttactagt gtagcagaaa caccgagggc tgataatgac tgtaatagtt tattcataat
     6601 gtaacctccg aatgataagt catttctgag ttagctttac tataccttaa tttatattat
     6661 ttacacatca tactatagtc ctaatcttac cctataaaga atgaaattta aatataaatt
     6721 tataaataat gaggaaataa ttattatttt gaaaatctgc cacaattgta aaattaataa
     6781 tttagggata atttgaaatc aaatataact ttattcgaca gtttgaactt aaatttttgt
     6841 gtatcaaaac aataagaaaa gggaggcgaa aatgataaag aaccactaat gattaactat
     6901 gtagtagtac tttatcatag tcacagcttc tgtgttgggg cccatcccca acttgcacat
     6961 tattgtaagc tgacttttcg tcagcttctt tgttggggcc ctgactagaa ttgaaaactt
     7021 gttacaagct tattttcatt caattaacta ctgtctatat aaaattgttg agccaagtac
     7081 aaagtgaaat gctcccttca aagccttcat ttattcaatg tctactttga agggagcatt
     7141 tgatcgatta atgtcccaga tttttcttgt tattcattgc aattgcaaaa taatatttaa
     7201 tgtgggttaa aaactttatt aaaactcagt tattagggag tgggacagaa atgatatttt
     7261 cgtaaaattt atttcgtcgt cccac
//
LOCUS       DALAGF010000126         7263 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.126, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000126 DALAGF010000000
VERSION     DALAGF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.126"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(11..1372)
                     /locus_tag="O2692_002456"
     CDS             complement(11..1372)
                     /locus_tag="O2692_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ4300060.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
     gene            1720..2841
                     /locus_tag="O2692_002457"
     CDS             1720..2841
                     /locus_tag="O2692_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HCZ4300061.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            2890..4158
                     /gene="icd"
                     /locus_tag="O2692_002458"
     CDS             2890..4158
                     /gene="icd"
                     /locus_tag="O2692_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCZ4300062.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            4548..5252
                     /locus_tag="O2692_002459"
     CDS             4548..5252
                     /locus_tag="O2692_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4300063.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            5252..6913
                     /locus_tag="O2692_002460"
     CDS             5252..6913
                     /locus_tag="O2692_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HCZ4300064.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKKIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
BASE COUNT         2625 a         1031 c         1355 g         2252 t
ORIGIN      
        1 aaacttgttg ttatttattt gatttagcag cgatacgttt atatcttaag tacataaatg
       61 ctaaaagtat aaaccaaatc ggaataaaat aaattgcacg tcttgtatca acattaataa
      121 ataataaccc gaacacaaaa atgaagaata caaatattaa gtagcccata tatttgccac
      181 ctaatagttt gtacgtagca tttttatgta gatctgggtt tttacgacta taattgatat
      241 atgcaatgat aatcagaccc catacaacta aaaataacac tgtagagatg gtagtcacat
      301 acgtaaatac ttttgtcgca tctgggaaaa tatagtttag taatgctgcc acaagtaata
      361 atgctgatga agcaaagatt gcaacatgtg gaacgccata tttattcgtc ttagaaaagt
      421 tcggaggtgc ttgttgttga cttgataaac cgaaaagcat acggctattt gagaatatac
      481 cactgttaca tgatgaagca gcagcggtta atactacaaa attaatcaag cccgcagcaa
      541 acggaattcc gatcaatgcg aataatttta cgaatggact gttatcagga tcaacttgct
      601 gccaagggat aatagacatg ataaccgcta acgccccaac gtaaaatatt aaaattctaa
      661 tgggtacact gttaattgct tttggaattg ttttaactgg atcttttgtt tccccagctg
      721 taacaccaat catttcaatt cctacaaatg agaatagtgc catttggaaa gacataaaga
      781 atccagaagc acctttagcg aatatgccgt gttcatatag atttgtgaaa ctggcatgcc
      841 cgaattgagt tttaaatgca aatagaatca tgacgaaacc aactactatt aaaccaataa
      901 ttgtcgctat tttaatgata gagaaccaaa attctaattc tccgaaaagt cttgcgctaa
      961 gtaggttgaa tgacattaat aacagtacac aaaatagtgc acttatccag tttggaattt
     1021 ctgggaacca aaagctaaca tattttgcca cagccgttac ttcagccata cctgtaataa
     1081 tccaacagaa ccagtatgtc catccggtaa caaatcctgc aaaaggccca atatatgtat
     1141 tggttacatc tgcgaaagat ttaaattcag tattctgtat aatgatttct cctaaacctc
     1201 gcataaacat aaataacata aatcctataa tgatgtatgt taatagaatt gaagggccgg
     1261 ttaatgcaat cgtttgacca gcacctaaga ataagcctgt accaattgca ccgccaattg
     1321 caattaattg tatgtggcga ttgctcagtt ccctttgtaa tttttcagcc ataatacatc
     1381 tcccttaaat atagatatgt ttattatgca cttatattga gatatataca attattttcg
     1441 gtaaaaatgt gtaaaattcc atgttaatat actttggttt ttataatcat atataataac
     1501 caattgaaaa tttaattcta ttgtaaaatt catggattat tcacatcttg aaaaagcttt
     1561 aatggtgcta tttgtggcta ttctgtgaca tttacataga tttacaaaaa aattgttgca
     1621 catataatgc cagtttttat atttcacaaa cgaaatgcgt ttagctataa tattagttga
     1681 aagccatttc ataaagaaac agtaaagggg aaatttatca tggcagaatt acaaagaggt
     1741 ttagaagggg ttatcgcagc ggagactaaa ataagttcaa ttattgaaag tcaattgact
     1801 tatgccggct atgatattga tgatctagct gaaaatgcgc aatttgaaga agttattttc
     1861 ctattatgga actatagatt gccaaacgaa gaagagcttg ctcatctaaa agggaaatta
     1921 aatcaataca tgacattaaa tcctcgtgtg tatacacatt ttgaggagta tgttacagat
     1981 cacgtgcatc caatgacagc attacgtacg tcattatcat atattgcaca tttcgatcct
     2041 gatgctgaaa atgaatcaga tgaaaatcgt tatgaaagag caatgcgtat acaggctaaa
     2101 gtagcatcat tagttacagc gtttgctcga gtaagacaag ataaagaacc acttaagcct
     2161 aatcctgact taagttatgc ggcaaacttc ctatatatgt tacgtgggga attaccaaca
     2221 gatatagaag tagaagcctt caataaagca cttattttac acgctgatca tgagttgaac
     2281 gcatctgcat ttacggcacg ttgtgcggta tcatcattgt cagatatgta ctcaggtatt
     2341 gtagcagccg taggttctct gaaagggcca ttacatggtg gtgcaaacga acaagttatg
     2401 acgatgttat ctgagattgg gtcaattgaa aatgttgatg cttacttaga tgaaaaattt
     2461 gctaataaag ataaagtaat gggcttcggt catcgtgtat ataaagatgg tgatcctaga
     2521 gcgaaatatt taagagaaat gagccgtcaa attacgaaag acgctggtcg tgaagaatta
     2581 tttgaaatgt cagtgaaaat ggaaaaacgt atggcagaag aaaaaggatt aattcctaat
     2641 gttgattttt atagtgcgag tgtttatcac tgtatggaaa tacctcatga cttattcacg
     2701 ccaatctttg ctgtaagtcg ttctgcagga tggattgctc atattttaga acaatataaa
     2761 gataatagaa ttatgcgtcc tagagcgaaa tatattggcg aaacgaatcg taagtatatc
     2821 ccgcttgaag aaagaaaata atcaatacaa attaaaaatg aagatgtaaa atttggaggt
     2881 aaaataacta tgactgcaga aaaaattact caaggaactg aaggattaaa cgtacctaat
     2941 gaaccaatta tcccatttat tatcggtgat ggaattggac cggatatttg gaaggcagca
     3001 agccgagtta tagatgctgc tgttgagaaa gcctataatg gcgaaaaacg cattgaatgg
     3061 aaagaagtgc tagctggcca aaaagcattt gatacaactg gtgaatggtt acctcaagaa
     3121 acacttgata caattaaaga atatttaatt gctgttaaag gacctttaac aacaccaatt
     3181 ggtggtggta ttagatcatt aaatgtggct ttacgccaag aattagattt atttacttgc
     3241 ttaagaccgg tacgttggtt taaaggagta ccatcacctg ttaaacgtcc acaagatgtt
     3301 gatatggtta ttttccgtga aaatactgaa gacatttatg ctggtattga atttaaagaa
     3361 ggtacaacag aagttaaaaa ggtaattgac ttcttacaaa acgaaatggg tgcgacaaac
     3421 attcgattcc cagaaacttc aggtattggt attaaaccag tttctaaaga aggaactgag
     3481 cgattagtta gagcagctat acaatatgct atcgataata accgtaaatc agttacttta
     3541 gttcataaag gtaatattat gaaatttaca gaaggctcat ttaagcagtg gggttacgat
     3601 ttagcattat ctgaatttgg tgatcaagta ttcacttggc aacaatatga cgaaattgtt
     3661 gaaaatgaag gcagagatgc tgctaatgct gctcaagaaa aagctgaaaa agaaggcaag
     3721 attatcatta aagattctat tgctgacatt ttcttacaac aaattttaac tcgtccagct
     3781 gagcatgatg ttgtagcaac tatgaacttg aatggtgact atatttcaga tgctttagct
     3841 gcacaagttg gtggtattgg tattgcgcca ggtgcaaaca ttaattatga aacaggtcat
     3901 gctatttttg aagcaacaca tggtacagct ccaaaatatg caggtttaaa taaagtgaat
     3961 ccatcttcag taattttaag ttctgtatta atgttagaac atttaggatg gcaagaagcg
     4021 gcagataaga ttacagattc aattgaagat acaattgctt caaaagttgt tacttatgac
     4081 tttgcccgtt taatggatgg tgctgaagaa gtttctacat cagcatttgc agatgaattg
     4141 attaaaaatt taaaataagc aaaataaaat taggtgagtc tagcaacttt aattgctagg
     4201 ttcgcctttt taaatgaatt gaagttggca acaggataga aataataaag agtcaccaat
     4261 gattgattat gtagtgtttc ttacacatta gacaaagcga atatgtacat aagcttagga
     4321 ataaatgagt aaaactcatg cataggtagt gggatagaaa tgctattttt acaaaattta
     4381 tttcgttgtc ccagagtaag tcatgcaaat gatagaagga aatataagaa gtattttatt
     4441 tatatgtaaa gtaatgtaaa aaatgtgtaa agataatgtg taggatgtta tttttataaa
     4501 tagttcggtt ataatgtaaa tataattata agttagggag gcataccatg tcgcaaaaag
     4561 tgttggtagt agatgacgaa cattcaatcg taacattact taaatataac ttagaaacag
     4621 ctggttatga agttgttgtc gcatttgatg gtgatgaggc tttagaaaag gtagaaagtg
     4681 aacagccaga tttaattatt ttagatgtta tgctacctaa aaaagatggc attgacgtat
     4741 gtaagactgt aagaacaaat aaaaatttag tccctatatt aatgttaacg gcgaaaaatg
     4801 atgaatttga tcgggtatta ggtttagaat taggtgccga tgactatatg actaagcctt
     4861 tttcacctag agaagttgtt gcccgtgtga aagccatttt aagacgttct caatttgtaa
     4921 aagaagtaga agagattgac gatgatatta ccatcggatc gatacgtatt agacccgatt
     4981 attttgaagt atataagcat aatgagttat tggagctaac accgaaagaa tttgaactct
     5041 tgctttattt gatagaaaga caaggcagag tcattacgag agagcatatg ttaaatacgg
     5101 tatggaatta tgaatttgca ggagactcaa gaattgttga tgtacatatc agtcatttaa
     5161 gagataaatt agaagataat cctaagaagc cacaacttat taaaactgtg cgtggcttag
     5221 gctataaatt ggaaagacct aaagaacaat gatgaagttt caccaccgct taatgttatt
     5281 gataagcagt atattaatta tcagttttgt tacattaggc attattattc acgcttccat
     5341 cacatatgtt ttatcagaaa aacacgaaca agctctagca acagatgcaa gatcatatat
     5401 ttatttagtc caagataatg agattgataa agttaaaaaa attgtaaaac agcaaaatat
     5461 agatttattg ataactaagc atgataaaaa ggtgtttagt agtggcaaac tgaaagctat
     5521 cccaaatagt gatagctata ttcaaaaaag ggacgttttt atttttaata agaaggttaa
     5581 cggttatcat gtatggatta aaggttacaa taacgatatt acagaaatgc attggacgtt
     5641 gtggaaatat cttattttga catgtttagt tgtactcatt tgtctgtatt ttgcaagccg
     5701 tagttttaag cgtacattaa tccgtcccat tcaagaagtt acttatgcaa cacaattgtt
     5761 ggcaaatggt tattatcaca tacgtgtacc tgaaagtaat gttgtagaaa ctaaagcgct
     5821 ttttgtgtct accaatgatt tagcacgacg ccttcaaaag cttaataatg agcaaaaaat
     5881 tcaatccaac cgtttaaaaa caacgattga aaatattcca agtgctattt taatgataga
     5941 tcgaaatgga aaaatcgttg ttgcaaataa agcgtattat gaacagttca acatctcgca
     6001 taatattgaa caagtaggct atcacggcta tgttaatact gaaattgagc aattaatttt
     6061 agaaagtttt aaagttgaaa aacctattta tgaacaattg gaagttgcaa taaaccaagt
     6121 ccatgccaag tactttgata tttcatgcgt tccgatttta actaggtctc aaaaaagttt
     6181 gcaagggatt ttagttgtga tgcatgatat aacaaatctg aaacaacttg aaaatctaag
     6241 acgtgaattt gtagcaaatg tttcacatga attaaaaaca cctattactt caattaaggg
     6301 gtttgctgaa acgcttattg atggtgcaaa aaatgatgcg gaatcattag acatgttttt
     6361 aaacataatt ttaaaagaat caaatcgaat agaatcttta gtaacagatc tgttagattt
     6421 atcgcatatt gagcaacata cagagttaga tacagactat atgaatttat cagatttaac
     6481 gcggcgtatt attgataata tgatgactca agccaatcaa aaaaatattt ccattcatac
     6541 tgatattgaa aaagatgtca ttgttaaagc acaagaaagt aaaattgctc aagttatcac
     6601 gaatttgtta acgaatgcaa ttaactattc ttatgaagat ggagatatta acgttcgtgt
     6661 gtatcgagat gactttcgtg tcattttcga agtacaagat tttggtatag gtattaaatt
     6721 agaagaccaa caacgtattt ttgaaagatt ttatagagta gataaagcac gaagtagaga
     6781 ttctggtggg acaggtcttg gattatcaat tacaaagcat atagtagagg cccaccaagg
     6841 caatattgaa gtgaatagtc aagttggcaa aggctcaacg ttcaaagtta ttctaaaaga
     6901 ttataaagaa taaaaattaa agtggtaaca gcgcgtgtat ttttcacgaa gttgttgctg
     6961 cttttttatt tcgttcaaat ctctattggt attaaattag agttaatgaa gtggaataaa
     7021 ttcggttgtc aattttgtca tttgtatatg tcgatttgta agttataagt aatagattgc
     7081 tgtgaatcaa gcaattttat aatatattta ttataataaa gaaattttag taacgcgctt
     7141 gaaatagatg cttaattaaa aagtaataag ttttatttga acggaaataa atcttaattt
     7201 aaacacttga atagacaagt gtaatcattc gtcatagaaa aagacgaggg gcgatttaga
     7261 taa
//
LOCUS       DALAGF010000127         7007 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.127, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000127 DALAGF010000000
VERSION     DALAGF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.127"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(136..705)
                     /locus_tag="O2692_002461"
     CDS             complement(136..705)
                     /locus_tag="O2692_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCZ4300065.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
     gene            915..1133
                     /locus_tag="O2692_002462"
     CDS             915..1133
                     /locus_tag="O2692_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HCZ4300066.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
     gene            complement(1214..2200)
                     /locus_tag="O2692_002463"
     CDS             complement(1214..2200)
                     /locus_tag="O2692_002463"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCZ4300067.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            2399..2575
                     /locus_tag="O2692_002464"
     CDS             2399..2575
                     /locus_tag="O2692_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HCZ4300068.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            2590..3192
                     /locus_tag="O2692_002465"
     CDS             2590..3192
                     /locus_tag="O2692_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCZ4300069.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            3425..3570
                     /locus_tag="O2692_002466"
                     /pseudo
     CDS             3425..3570
                     /locus_tag="O2692_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
     gene            4110..4211
                     /locus_tag="O2692_002467"
     CDS             4110..4211
                     /locus_tag="O2692_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300070.1"
                     /translation="MKFAANKLTLTIFRKFCINNEKEIWIKYEEVFL"
     gene            4221..4493
                     /locus_tag="O2692_002468"
     CDS             4221..4493
                     /locus_tag="O2692_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCZ4300071.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNWWVNRAYYNIY"
     gene            4537..6501
                     /locus_tag="O2692_002469"
     CDS             4537..6501
                     /locus_tag="O2692_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCZ4300072.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRIDQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKSKAATITPFNAKY
                     NIHYLSDDEVLKKDIKGSYFVKDKNFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIK
                     QMNIVVPLSSLLIVYFIYYIFEKNINFKAYAIKYLNGFTLRKIIFENFSKKCTYWVTL
                     IITQILLTTSVLWILNYTGNLDLFILRIVLLSCLFILTISVINLWTFLMLLNLNIANM
                     IKGKQHFKTIRFINTVCKSILLVLIANVMIENTSVIKDLNKIKETEKYWNVLDDYYTI
                     EFAPYHETKQSLIDNMVRSEQLVKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVN
                     NQFWSIYYKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAYHSWFEFVQNKNNKEN
                     KLSIQFINKNDCRIFSFDARDSRHLSFIEAPIIVNVQASDLSNDFYYAMISQGGYLFK
                     NYDALVKNIEKYHLDGEISGITNYKDSVMEMYHENNLKLTVLNFSQIIIAIILIIIIL
                     FDVKYYFEQHRKLLVIKKLYGYSTLRANYQYLLINNIVVIFIGILTNVILHSHYIMML
                     FATILVVQILLQICSLYYHGRRFNEVIKEF"
     gene            6504..6824
                     /locus_tag="O2692_002470"
     CDS             6504..6824
                     /locus_tag="O2692_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCZ4300073.1"
                     /translation="MKFIIAILLGLILSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKE
                     VRQE"
     gene            6821..>7007
                     /locus_tag="O2692_002471"
     CDS             6821..>7007
                     /locus_tag="O2692_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ4300074.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNG"
BASE COUNT         2512 a          935 c         1050 g         2510 t
ORIGIN      
        1 tgtatgtgtt tgaatatcaa gcacaaatat tattataaca agattaagtt aatttgcaat
       61 agatacctta aaaaaatatg cagaacattt acactatatt tataaaacat tccgcatatt
      121 ttaaaaataa acaatttatt tatcttcaaa taaagaatag cgtgtcaatg cttcaaacac
      181 attcaatgtg gctttattat aatctattgg ttggtcggga ttttttgata tcatgcgaga
      241 ttgtttatct accatgtaat agtaaaaaat ggaattgtta tattgatgcc ataaactgtg
      301 gtatgaaaca tgaagaatga ttgattcatt attaataaat gtcactttac atttacgatt
      361 ttttaattct ttaatacttt cgatataatg catatttaac caaatatttt cattttgtct
      421 gtcagaatgt gttgggaaaa aataagttgg aaataatggt gttagtaaaa taggtggttt
      481 actagaaatg ccagtaatgc ggtttgtctc tgctttcttg ccaagataag tattaccata
      541 aaatttgcaa gagcgttcaa tgattttctg gactttaaaa ggattttgaa tacgcgtttt
      601 gtcaaaccga attatttcac taccgtttct ttgatcgtca tcatcaaatg ctggtcgaat
      661 aaccatgtct cctttgcgta tcacataaat attttgagaa tacataagca aaacctcgct
      721 ttattaagtt ttaaaccatt aatataaaaa tacaattaat gatataaaga tattttataa
      781 taaaattctg agttgtcaag ttattatttt ggtgaaaatg tacaataatt tttaactaat
      841 agtcagaata ttcattgatt tcaaaattag aaaaatgtat acttattata aattaaagaa
      901 aggtagtggt ttctatgaaa tacaatacta atgttaaaca tacaacttta gaagcgtttg
      961 tcacaactgt caatgatttg ggtattgaat taattatcaa tgaagcactt cgagaggtaa
     1021 gaaaacgaca gctcatagaa cttatagatg acgcactcgt caataaagat gaagcagcat
     1081 ttaatcaata tacggcagaa tacaaaaatt tggaggcatt tctcggtgaa taacattgat
     1141 tcgaattcca taacgcaatc ataaaaggac tattcttaac atctcataca tgtaagaata
     1201 gtcctttaat cgtttatgac attaatctaa ttaattcatg tctatctatg tcaccgaaat
     1261 aatgatataa atcatattct gatagtgctt cttctatgtg ctcaaagtcg tgtagacagc
     1321 caactaatgc attttcaaga tcagtgacat ctccgacacc aaagaaatca ccgaatattt
     1381 ttgcatgttc gattttacct cgtttaacat caaacttaat ttgtacaaat cctttttcaa
     1441 atttttcctc gcgttcaaag ttatatttag ggtttctacc ataattccat tcccaagttc
     1501 tatatttgtc gttacttaac ttttcaatat tttcccaatc ttcatccgtt aatttatatt
     1561 cttctacttc agtttctcca aagatagttt tcaagatgat ttttttaaat tcttcaattt
     1621 ctaaagggtc atttaaaaat tcttgaatgt ttgctacccg tttacgaaca gatttaatac
     1681 cttttgattt aatcttagct ggattaactt ttagtgcatt ctgaacttcg tctaaatcac
     1741 tatttaacat caacgtacca tgactaaaca ttctattttt aactttaacc atagcattcc
     1801 ctgagatttt cgcttgtcca acttgaatat cgttacgacc agttaattca gcatttacgc
     1861 ctaaagattg taatgcttga acaattggtt cagtgaattt ttggaaattg tggaaactgt
     1921 taccatcatc atctgttata aaactaaagt ttaaattgcc agtatcatga taaacagcgc
     1981 caccaccaga aattcttctt actacatcga tgttgtgagc atcgatatat gtctgattta
     2041 cttcctctat cgtattttga ttctttccaa caatgataga tggtctattt atgtaaaata
     2101 aaaagtaact ttcttctgct ggtaaatttt ttaaaacata ttcttccatt gctaagttta
     2161 aagttggatc tgtaatatta ttattactaa tgaatttcat tacaatctct cccttatatc
     2221 tatatatatt ctctacttat ttatgcctta actttgctca aaatgccaat tatatactca
     2281 caaatcattc taaatatcgg cttaaagtta cattttatta tttttgattg ttaatagttt
     2341 atttgttaag tgaattttag tacaatataa tttagttatg atttaaggag gacttaaaat
     2401 gactgttgca gaagtgggta acattgttga gtttatggat ggattaagag gtcgtgttga
     2461 aaaaatcaac gataactctg ttattgttga cttaacaatt atggaaaatt ttaatgacct
     2521 tgatttaccg gaaaaaactg ttatcaatca taaacgatat aagattgttg aataagaagg
     2581 taagttataa tgaataaaat ctcgaaggct ttaacttggt ttattataag tttcattata
     2641 tttcatctca tattatttat tatgtggggc gaacaccaag aatactggta tttatataca
     2701 ggtataatgc taattgctgg tatcagttat gtattttatc aaagagatat tgaatctaag
     2761 cggttgctta catcaattgg tgttggtatt attacggcaa ttattttaat tatgcttcaa
     2821 cttttattct cacttataac ttctaattta agttatagtt cattaattaa agaattagca
     2881 agaacaggtg tcaattggaa gtggcaaatg ttagtaactt tactttttgt cattccatgt
     2941 cacgagttat atatgagaac tgttttacaa aaagaattaa cacatttttc aataccgaaa
     3001 tgggtagcta tattaataac tgcaatttgt tctagttcat tatttattta tttagataat
     3061 tggtggatag taacatttat atttgttgca caagtgatat tatcattaag ttatgagtat
     3121 actcgacgta tagcaacaac atccgttgca caaattgtag ctattatatt gttattaatt
     3181 tttaatgctt aaataagctt ttcttagacg tatgtgttct aagaaaagct tttttgctta
     3241 agaatagcaa tacgaagatt cgaggcatgc ttcattatag tggtaatatt ttttaacagc
     3301 aagcggttat tattgagatt aggttttagt cagtttttat tactaaatat tttataaaaa
     3361 tggtataata tttttgagca agttgaattg atggtggcta tctgagtaaa ggggggtggt
     3421 gcctatggca ttacttaaca cttttgaaag gaaaagccta ttgtgatatc tattgcaaac
     3481 gcattacatt taatgttaag tttcggtatg tttatcgtca ctttcattgg tatagtagta
     3541 gcaataataa atttaagcaa taaaaaataa ccatcatttc aaaactttga catagaatga
     3601 tggttaaaga aaatctcatt tagtcaccgt ctttttaacg ggctcattag gcgacatgtt
     3661 cctgcatgtc gtctttttac atttttatag taacatatct tttatttaat tgtctatttt
     3721 tattagatgt gtaacaaaat tttgaagtat tttccgtata ttgcctgcta aacatgtcag
     3781 atttgaaagg aaaatcatac tgtggtagaa atcaattatg attatatggt agtacttata
     3841 aataagtgat ttctattgaa tatgtaatga agttcatata ttaatataac tttcattgat
     3901 atagaagtcg caatgattaa tttaaacaat aaaaaaacaa ccatcatttc caaactttga
     3961 caacgaatga tggttaaaga aaatctcatt tagtcaccgt ctttttaacg ggctcaatag
     4021 gcgacatgtt cctgcatatc gtctatttat acactgcaca atttaataat aatacaatta
     4081 tactttgatg tccatttaac ataaagtggt tgaaatttgc agcaaataaa ttaaccttga
     4141 ctattttcag aaaattctgt ataaataacg aaaaagagat atggattaaa tatgaagagg
     4201 tgtttttata gaagaataaa atttttggca ctatattata tttaacttta gcacttggat
     4261 tatcaacagc agcttatgca tctacagaat acgcagaagg aggcacttgg agtcatggtg
     4321 tcggcagtaa gtatgtttgg tcgtattatt atcatggtca taaaggacat ggtgcaacag
     4381 ctattggaaa atatagatca tttagtggtt atacaagagc tggtgtaaaa gcaaaagcat
     4441 cagctactaa acataattgg tgggtcaata gagcgtatta taacatttat taaatactaa
     4501 aaaatcatag aaaataaaca acgcaagagg tacaacatga aatggttcaa attaatactt
     4561 gatgttacaa tcttcattct gattgctata ttgttatttg tttatacata taaagaaaac
     4621 gaagaaatat tgcctgatac taaataccct atagcggtaa ctgactggaa taaaaaatat
     4681 agtaaaaatg agatttataa acgtatagat caattcgcta aaaatgagaa cgtagcaatc
     4741 tataaatcaa cttcaaatta tacaaacaaa aacgtagata aagatatata tgtatttaat
     4801 aaatcaaaag cagcaactat cactcctttt aatgctaaat ataacattca ttatttaagt
     4861 gacgacgaag tattaaaaaa agatatcaaa gggagttatt ttgtaaaaga caaaaatttt
     4921 gacgtgtcta aattcataaa ttttttaaaa gaatatggtg ttactgctga atcatacaaa
     4981 atagatcata tgatgattgc cgttggtgtc attaaacaaa tgaatatagt agttccatta
     5041 tcctcacttt taatcgttta ttttatttat tatattttcg aaaagaacat taatttcaaa
     5101 gcgtatgcga ttaagtattt aaatggtttt acattaagaa aaataatttt cgaaaatttt
     5161 tcaaagaaat gcacgtattg ggtaacgtta atcataactc aaattctttt aactacaagt
     5221 gtactttgga tattaaatta cacgggtaat ttagatttat tcatattaag aatagttctg
     5281 ctttcatgtc ttttcatttt aacgattagt gttattaatt tatggacttt cttaatgtta
     5341 ctaaatttaa atattgcaaa tatgattaaa ggtaagcagc actttaaaac aattcgtttt
     5401 attaatacag tttgtaaaag tattctctta gtactaatag ctaatgtaat gatagaaaat
     5461 actagtgtta ttaaagattt gaataaaata aaagaaactg agaaatattg gaatgtatta
     5521 gatgattatt acacgattga atttgcacct tatcacgaaa caaaacaaag tttgattgat
     5581 aatatggtgc gatcagaaca attagtaaag gctagtgaag cagaaaataa tgcgatttta
     5641 ttcaaaccaa agggtgactc cgttgacaat gacaactttt cgcctgatga ggggaatgta
     5701 atattagtaa ataatcagtt ctggtcgatt tattacaagc agtttcaacc tgatattccg
     5761 ataaaaaatc aaaaaaataa tgtcgaagta attattccac aaaagtttca tgcaatgcgt
     5821 aatgaaatca atcaagcata tcattcatgg tttgaatttg tacaaaataa aaataataaa
     5881 gagaataagt tatctataca gtttatcaac aaaaatgatt gtcgaatttt ttcatttgat
     5941 gcacgagata gtcgccattt gtcatttata gaggcgccaa tcattgtgaa tgttcaggca
     6001 tcagatttat cgaatgattt ttattatgcc atgatcagtc aaggcgggta tttattcaaa
     6061 aattatgacg cgctagtaaa aaatattgaa aagtatcatc ttgatgggga aatcagtgga
     6121 ataaccaatt ataaagatag cgtgatggaa atgtatcatg aaaacaattt gaaattaaca
     6181 gtactcaact tttcacaaat cattatcgca atcattttaa taattattat tttatttgat
     6241 gtgaaatatt attttgaaca gcatcgaaaa ttactcgtaa tcaaaaagct atatggttat
     6301 tcaacattaa gagccaatta ccaatactta ttaataaata atatagttgt tatttttatt
     6361 ggaatattga cgaatgtaat tttacattct cactatataa tgatgttatt tgcaacgatt
     6421 cttgtcgttc aaatattatt gcagatttgc agcctatact atcatggccg acgttttaat
     6481 gaagttatca aggagtttta actatgaaat ttatcattgc aatattatta ggattaatat
     6541 tatccattac tattgctttt acaatcatac atcatcctat acttgatcgt tttaatcctt
     6601 tcttaaaaac ggagtatagt tatgccaaag tgccaaaagg tacgcaacaa tatgttaata
     6661 ttacggctta tagtgaaaga ggggaaaagc ttgattataa attaacattt aatggatttt
     6721 cacctagtag aacgtatgtc gaaataaagc ataaagggca atatgtcata tcgatcacat
     6781 atgttgaaaa agaggataca ccaaaagagg taagacaaga atgatagaat taaaagattt
     6841 aaccatacaa aaaggtaata cacatatttt gaagaaactt aatttgaaat ttcaatgtgg
     6901 aaaatcatat gcacttattg gtaaaagtgg atgcggtaaa tctacattat taaatactat
     6961 tgctggactt gaaaaaacag gaaaacaata tgtctatttt aatggtc
//
LOCUS       DALAGF010000128         6995 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.128, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000128 DALAGF010000000
VERSION     DALAGF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.128"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..825
                     /locus_tag="O2692_002472"
     CDS             <1..825
                     /locus_tag="O2692_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCZ4300075.1"
                     /translation="LNVFFRMTLKYNQILYIALIQFLGLLIGLFLYYLTQNWIVCFIT
                     SELFATIYTLVKLRGLTIGEYQSEDNNVVKDYVMLLSTNSLNNLNLYLDRLILLPIIG
                     GTAVTISFLSTFIGKMLATFLYPINNVVLSYISVNESDNIKKQYLKTNLIAIAALCLV
                     MIICYPITIIIVSLLYNIDSSLYSKFIILGNIGVLFNAVSIMIQTLNTKHASITLQAN
                     YMTLHTITFIFITILMTIAFGLNGFFWTTLFSNIIKYVILNIIGLKSKFINKKDVD"
     gene            826..2031
                     /gene="cap8L"
                     /locus_tag="O2692_002473"
     CDS             826..2031
                     /gene="cap8L"
                     /locus_tag="O2692_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HCZ4300076.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQSQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKALVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            2042..2599
                     /gene="cap8M"
                     /locus_tag="O2692_002474"
     CDS             2042..2599
                     /gene="cap8M"
                     /locus_tag="O2692_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HCZ4300077.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            2599..3486
                     /gene="capN"
                     /locus_tag="O2692_002475"
     CDS             2599..3486
                     /gene="capN"
                     /locus_tag="O2692_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HCZ4300078.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGHVGKSDQVDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSMLNKYFNKLSVFRKLFGNLIYSNTLYENNNALEIIPG
                     KMSLVIADIMDETTTKDKA"
     gene            3540..4802
                     /gene="cap8O"
                     /locus_tag="O2692_002476"
     CDS             3540..4802
                     /gene="cap8O"
                     /locus_tag="O2692_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HCZ4300079.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILSFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTEACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKVLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            4879..6024
                     /gene="cap8P"
                     /locus_tag="O2692_002477"
     CDS             4879..6024
                     /gene="cap8P"
                     /locus_tag="O2692_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HCZ4300080.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK"
     gene            complement(6089..6415)
                     /gene="isdI"
                     /locus_tag="O2692_002478"
     CDS             complement(6089..6415)
                     /gene="isdI"
                     /locus_tag="O2692_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HCZ4300081.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(6422..6805)
                     /locus_tag="O2692_002479"
     CDS             complement(6422..6805)
                     /locus_tag="O2692_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HCZ4300082.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRVGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
BASE COUNT         2441 a         1036 c         1286 g         2232 t
ORIGIN      
        1 ctgaatgtat tttttaggat gactttaaaa tataatcaga ttttatatat tgctcttatt
       61 caatttttag gtttgctgat aggactattt ctatattatt taacccaaaa ctggattgtt
      121 tgttttatta ccagtgaatt gtttgcaacg atatatacat tggttaaatt acggggatta
      181 actataggcg agtatcaaag tgaagataat aatgtggtaa aagattatgt gatgctactg
      241 agtacaaata gccttaataa tttgaatctc tatttagata gattaatctt attaccaatt
      301 ataggtggaa cagctgtaac tatatcattt ctttcaacat ttattgggaa aatgttagct
      361 acatttctat atccgattaa taatgtagta ctttcatata tttctgtaaa tgaaagtgac
      421 aatataaaga agcaatattt gaaaactaat ctaattgcta tagctgccct atgtttagtc
      481 atgattatat gttatccaat tacaataatt attgtctctt tactgtataa cattgattca
      541 agtttatatt cgaagtttat tattttaggt aatataggtg ttttattcaa tgcagtgagt
      601 attatgatcc aaactttaaa tacaaaacac gcatcaataa cattacaagc gaattatatg
      661 acgcttcaca cgattacatt tatattcata actattttaa tgacaattgc gtttggtcta
      721 aatggattct tttggacaac gctgttcagc aacattatta agtatgtgat tttaaatatt
      781 ataggtttaa agtctaaatt cattaataaa aaggacgtcg attagatgag tgaaaaaaag
      841 attttgattt tatgtcagta tttttatccg gaatatgtat cttctgcgac gttaccaact
      901 caattggcgg aagatttaat tgcgaatcac attaatgtcg atgtcatgtg tggatggcca
      961 tatgaatata gtaatcataa acaggtttct aaaaccgaga tgcatcgtgg tattcgcatt
     1021 cgacgtctca agtattcgag gtttaataac aaaagtaagg ttggaaggat catcaatttc
     1081 tttagtttat tttcaaaatt cgtgattaat atacctaaaa tgttgaaata tgatcagatt
     1141 cttgtttact ctaatccacc aatcttgcca ttaataccag acgttttaca cagactgctt
     1201 aagaaaaaat attcttttgt ggtgtatgat atagcacctg ataatgcgat taagacaggt
     1261 gcaactcgtc caggtagcat gattgataag ctgatgcgtt acattaatag acatgtctac
     1321 aagaatgctg aaaatgtcat tgtccttggt acggaaatga aaaactactt actaaatcat
     1381 caaatttcta aaaatgctga caatatccat gtgattccta actggtatga catgcgtcaa
     1441 ttacaagaca atcgtatcta taatgacaca tttaaagctt accgtgagca atacgacaaa
     1501 attttattgt atagcggtaa tatggggcag ttacaggata tggagacact tatctcattt
     1561 ttaaaattaa ataaggatca gtctcaaacg ttaacaatac tttgtggtca tggtaagaaa
     1621 tttgcagatg tcaaaacggc aatagaagac catcgtattg aaaatgttaa aatgtttgag
     1681 tttttaacag gtacagacta tgctgacgta ttaaaaattg cggatgtatg tattgcatcg
     1741 ctgattaaag aaggcgtcgg tttaggcgtg ccgagcaaga attatggcta tcttgcagct
     1801 aagaaagcgt tggtactcat catggataag caatctgata tcgttcaaca tgttgaacaa
     1861 tatgatgcgg gtatccaaat tgataatggc gatgcacatg ccatttataa cttcatcaac
     1921 actcactcga gtaaggaatt gcacgagatg ggtgagcgcg cacatcaact gtttaaagat
     1981 aaatatacga gagaaattaa tactatgaag tattacaatc tgttgaagtg aggagataat
     2041 tatgaagcga ttattcgatg tagtgagttc aatatatggt ttagtagttt taagtccgat
     2101 tctgttaatt acagcattac taattaaaat ggaatcacct ggaccagcca ttttcaaaca
     2161 aaaaagaccg acgattaata atgaattgtt taatatttat aagtttagat caatgaaaat
     2221 agacacacct aatgttgcaa ctgatttaat ggattcaaca tcgtatataa caaagacagg
     2281 gaaggtcatt cgtaagacct ctattgatga attgccacaa ttattgaatg ttttaaaagg
     2341 agaaatgtca attgtaggtc ctagaccagc gctttataat caatacgaat taatcgaaaa
     2401 acgtacaaaa gcgaacgtgc atacgattag accaggtgtg acaggactag ctcaagtgat
     2461 ggggagagat gatatcactg atgatcaaaa agtagcgtat gatcattatt acttaacaca
     2521 tcaatctatg atgcttgata tgtatatcat atataaaaca attaaaaata tcgttacttc
     2581 agaaggtgtg catcactaat gagaaaaaat attttaatta caggcgtaca tggatatatc
     2641 ggtaatgctt taaaagataa gcttattgaa caaggacatc aagtagatca aattaatgtt
     2701 aggaatcaat tatggaagtc gacctcgttc aaagattatg atgttttaat tcatacagca
     2761 gctttggttc acaacaattc acctcaagca aggctatctg attatatgca agtgaatatg
     2821 ttgctgacga aacaattggc acaaaaggct aaagctgaag acgttaaaca atttattttt
     2881 atgagtacta tggcagttta tggaaaagaa ggtcatgttg gtaaatcaga tcaagttgat
     2941 acacaaacac caatgaaccc tacgaccaac tatggtattt ccaaaaagtt cgctgaacaa
     3001 gcattacaag aattgattag tgattcgttt aaagtagcaa ttgtgagacc accaatgatt
     3061 tatggtgcac attgcccagg aaatttccaa cggttaatgc aattgtcaaa gcgattgcca
     3121 atcattccca atattaacaa tcagcgcagt gcattatata ttaaacatct gacagcattt
     3181 attgatcaat taatatcatt agaagtgaca ggtgtgtacc atcctcaaga tagtttttac
     3241 tttgatacat cgtcagtaat gtatgaaata cgtcgccaat cacatcgtaa aacggtattg
     3301 atcaacatgc cttcaatgct aaataagtat tttaataagt tgtcggtctt tagaaaatta
     3361 ttcggcaatt taatatacag caatacgtta tatgaaaata ataatgcact tgaaattatt
     3421 cctggaaaaa tgtcacttgt tattgcggac atcatggatg aaacgacaac caaagataag
     3481 gcataagtca tctattaaat aaaatcaaca tacaaatcgt tttatttgga ggttatagta
     3541 tgaagttaac agtagttggc ttaggttata ttggtttacc aacatcaatt atgtttgcaa
     3601 aacatggcgt cgatgtgctt ggtgttgata ttaatcagca aacgattgat aagttacaaa
     3661 gtggtcaaat tagtattgaa gaacctggat tacaagaggt ttatgaagag gtactgtcat
     3721 cgggaaaatt gaaggtatct acaacgccag atgcatctga tgtttttatc attgccgttc
     3781 cgacgccgaa taatgatgat cagtaccggt catgtgacat ttcgctagtt atgcgtgcat
     3841 tagatagtat tttatcattt ttagaaaaag gaaataccat tattgtagag tcgacaattg
     3901 cgcctaaaac gatggatgat tttgtaaaac cagtcattga aaatttaggg tttacaatag
     3961 gtgaagatat ttatttagtg cattgtccag aacgtgtact gccaggaaaa attttagaag
     4021 aattagttca taacaatcgt atcattggcg gtgtgactga agcttgtatt gaagcgggta
     4081 aacgtgtcta tcgcacattc gttcagggag aaatgattga aacagatgca cgtactgctg
     4141 aaatgagtaa gctaatggaa aacacatata gagacgtgaa cattgcttta gctaatgaat
     4201 taacaaaaat ttgcaataac ttaaatatta atgtattaga tgtgattgaa atggcaaaca
     4261 aacatccgcg tgttaacatc catcagcctg gtccaggtgt aggcggtcat tgtttagctg
     4321 ttgatccgta ctttattatt gctaaagacc ctgaaaatgc aaagttaatt caaactggac
     4381 gtgaaattaa taattcaatg ccggcctatg ttgttgatac aacgaagcaa atcatcaaag
     4441 tgttgagcgg gaataaagtc acagtatttg gtttaactta taaaggtgat gttgatgata
     4501 taagagaatc accagcattt gatatttatg agctattaaa tcaagaacca gacatagaag
     4561 tatgtgctta tgatccacat gttgaattag attttgtgga acatgatatg tcacatgctg
     4621 tcaaagacgc atcgctagta ttgattttaa gtgaccactc agaatttaaa aatttatcgg
     4681 acagtcattt tgataaaatg aagcataaag tgatttttga tacaaaaaat gttgtgaaat
     4741 catcatttga agatgtatcg tattataatt atggcaatat atttaatttt atcgacaaat
     4801 aaaatgtgtc aaactagggc atacatgatt aaggaaagat aagctgtcat gtgtttgaac
     4861 ttcagagagg ataatgttat gaaaaaaatt atggttattt tcggtacgag acccgaagca
     4921 ataaaaatgg caccattagt aaaagaaatt gatcataatg ggaactttga agcgaacatt
     4981 gtgattacag cacaacatag agatatgtta gatagtgtgt taagtatatt tgatattcaa
     5041 gctgatcatg atttaaatat tatgcaagat caacaaacat tagcaggcct tacggcgaat
     5101 gcacttgcta aacttgatag catcattaat gaggaacaac cggatatgat tttagtacat
     5161 ggtgatacta caacgacttt tgtaggaagt ttggcagcat tttatcatca aattccggtc
     5221 ggacatgtag aagctggact tcgaacacat cagaaatact caccatttcc tgaagagtta
     5281 aatcgagtca tggtaagtaa tattgctgaa ttgaattttg cgccaacagt aattgcagct
     5341 aaaaatttac tttttgaaaa caaagacaaa gagcgtatct ttattactgg aaatacagtt
     5401 attgacgcat tgtcaacaac agttcaaaat gattttgttt caacgattat taataaacat
     5461 aaaggcaaga aagttgtttt actaacagcg catcgtcgtg aaaatattgg ggaaccgatg
     5521 catcagattt ttaaagcagt aagagatttg gcagatgaat ataaagatgt tgtcttcatt
     5581 tatccaatgc atcgtaatcc aaaggtaaga gcgattgccg aaaaatattt atctgggaga
     5641 aatcggattg aattaattga gccattagat gcgattgagt tccataattt tacaaatcaa
     5701 tcgtacctcg tgctgacaga ttctggtggt attcaagagg aggctcctac atttggaaaa
     5761 cctgtgttgg tattaaggaa tcatacagag cgtcccgaag gcgttgaggc gggaacatcg
     5821 agagtaattg gcacagatta tgacaatatt gttcgaaatg tgaaacaatt gattgaggat
     5881 gatgaagcgt atcaacgtat gagtcaagcg aataatccat atggtgatgg acaagcatca
     5941 cgacgtattt gtgaagcaat agaatattat tttggattgc gcacagacaa gccggatgaa
     6001 ttcgtacctt tacgtcacaa ataataaaaa acccctaatc atgaagttgg tttagacaac
     6061 cagcggtgac taggggtttt taatatattt atttttgata gtggtagcca atatcatatt
     6121 tgaatacttt atttgataat attggacttt gctgtccatc gtcatcactt tttaaacgta
     6181 catttttatg agcttcttta aatacatcgg aattcaacca attattaaag ctatcttcag
     6241 attcccaaat agttaagatt ttaacttcgt ctgtatcctc ggtatttaat gttttagtga
     6301 caaacatttg ttggaagcct tcaatagttt caataccttg tctattgtaa aaacgttcaa
     6361 tcgtttcttc cgcactgcct ttttgtaatt gtaatctatt ttctgccata aacatgggca
     6421 atcactcctc tattttatga tttgatttgg gtaatgtttt tacaaatgta aagagtacag
     6481 cggtttgtat gataaccatt atgattaatc ctacacggac tgcaagaaca tccaccatat
     6541 aaattgaaaa acctattaca atgtataagc taattaaaat tttaattttc tgttgtagcg
     6601 tgtagcctcg atgtaaataa aagttttcta catattcttt ataaattttt tgattaataa
     6661 gccaattgta aaagcgatct gaacttcgag caaaacaaaa aactgctacg agtaaaaaag
     6721 gggtcgttgg cagtaaaggt aatacggcac ctgcaatacc aagcgctgta aatattaagc
     6781 caatgacgat taaaataagt cgcattgaaa aaactccatt ctagtactaa tgcgcatgta
     6841 atattgtttt agtaatataa ctcatgctaa atataatgtg tatgataagt gcaatgactc
     6901 agtaaaatga aacgatgttg aattatcctt gtcacattaa cgcattttaa gcgcgacttt
     6961 cataacaacc aaactattta atgagaatta ttctc
//
LOCUS       DALAGF010000129         6938 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.129, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000129 DALAGF010000000
VERSION     DALAGF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.129"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(206..901)
                     /locus_tag="O2692_002480"
     CDS             complement(206..901)
                     /locus_tag="O2692_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4300083.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            complement(901..1842)
                     /locus_tag="O2692_002481"
     CDS             complement(901..1842)
                     /locus_tag="O2692_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCZ4300084.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKNPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            complement(1859..2494)
                     /locus_tag="O2692_002482"
     CDS             complement(1859..2494)
                     /locus_tag="O2692_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4300085.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIVLTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            complement(2491..3717)
                     /locus_tag="O2692_002483"
     CDS             complement(2491..3717)
                     /locus_tag="O2692_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4300086.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAEISGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDAIFVVDSNNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQTEHVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            complement(4044..4163)
                     /locus_tag="O2692_002484"
     CDS             complement(4044..4163)
                     /locus_tag="O2692_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300087.1"
                     /translation="MGGCPSVTMDACALLQKFDFCNNISHFRHFFAIKQPIER"
     gene            complement(4243..4383)
                     /locus_tag="O2692_002485"
     CDS             complement(4243..4383)
                     /locus_tag="O2692_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300088.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCVCHGQHFDVGIPLQA
                     WE"
     gene            complement(4387..4988)
                     /locus_tag="O2692_002486"
                     /pseudo
     CDS             complement(4387..4988)
                     /locus_tag="O2692_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
     gene            5168..6805
                     /locus_tag="O2692_002487"
     CDS             5168..6805
                     /locus_tag="O2692_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCZ4300089.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
BASE COUNT         2155 a         1357 c         1035 g         2391 t
ORIGIN      
        1 aataaaacat ctcatttttt agtattttta caataattaa cgctttttat ccaaaaactt
       61 tcatgataat aattgctatt ttagtgtaaa cgcatacaaa cacaacctat aaaaaggcta
      121 ctcaaagatt atttgagctc cttgagtagc ctcatttttt ataaatcatg aaacgaaatc
      181 gctaaattat aaacatcttc tactattaca tattaatact ttgcggccga tgattagatt
      241 gatttttacg atgtcgtgtt gttgggtcta atcgtttttc taaaaatctt aatagtacat
      301 caatgacgat tgcaatgata gcaatcggaa tcgcacctgc taaaataaac gttgtgccat
      361 ccgtcgcatt tgtaccacga atcacaatgt caccaagcgt aggtgctcct ataaatgatc
      421 caacggcaac aacacctatc gcaacaacca aggcaatacg aatgccaccg ataataaccg
      481 aaacagataa cggtaattca atcattcgta gcacttgatt gcgtgtcatt cccatacctt
      541 tgccagcatc cttaatattc gcatcaacac tagctatacc agtataagtg ttttttataa
      601 ttggaagtaa cgcatataaa aacactgtta aaactactgt ttctgaacct aagcccatga
      661 ctaacattaa aatagctaac attgcaatga ctggaactgt ttgaattata tttgcaattg
      721 taattacaaa tccagaaagt tttgtgtatc ttgcaagcaa gattcccaat ggaataccaa
      781 ttaaagctgc aaacagcaca ccatagacag acattaataa gtgtttgaaa aataaatccc
      841 atagataacc aaagttcgta acataataat tgaataattg ctgtaataaa ttaccttcca
      901 ttacttatga ccacctttct gtttatcaaa atagtggtgt tttttcaaaa attcttctgc
      961 gacgacagca ggttctttac ctttaccatc cgcttcataa ttcaagcgtt gcatctctga
     1021 agtcgaaatc tttcctgtca acttattaat cgtcgtttta agttctgggt gttgccgtaa
     1081 taattcattt gttgcaacag cactcgcagc ataaggtggg aaaaattgtt tatcatcttt
     1141 aagtactttc aaatcatacg ccgcaattcg accatctgta gaataaccta atgcaacgtc
     1201 taacttctct gagtttaatg cgtcgtagac tagacctatt tgcattggtc tcactgtacc
     1261 aaagtcaaaa ccatactctt ttttaaatcc ttcatagcca tcgcctttac gattcatcca
     1321 tgaactatcc atacctaaac gtaaatcttt actatgcttt gctaaatctg aaactgtctc
     1381 taaatgatat tttttagcgg tttccttcgt taccatgaat gcatacgtat tcgcaaaacc
     1441 atacgaatca aaaaacgttt gatcaaattt ctttttaaat ccttgttgtg ttgctatcat
     1501 tgctttctta ggatttttaa ttggtgcttc cttcaaagct cccgttaaat ctgtgccatt
     1561 atatctaaca cctgatatat tagcatcccc attaattaag gcattatgtt gaatcgtact
     1621 tgaccctaaa ttatttacta atgttggctt tatcttaccg tgtgtatcat gctctattaa
     1681 caaccgtaac atatgtgaaa taatttgcga ttcgcttgtt gataatgctg taattttgac
     1741 atcatttttc gtgctcttac tacctagtcc gggcaaacta catccagata atacggtaag
     1801 cgataagaca aacacgacaa gtatatattt aattttcttc attatcttag cctcctaatt
     1861 atccagatac ttttaagcct ttgggaacta cccatttttc aactaaagct aataaggcat
     1921 caacacctaa tgctagtgca gtaacgagta ccgttgcagt tacaatcatc agtggatcat
     1981 ataaatttaa accattgaaa atgaaatcac ctaatccacc cgcacctaca taacttgcaa
     2041 gtgtagccca actaattaca tacacagatg acaaacgaat gccaccaatg ataagcggca
     2101 atgctaacgg caattcaaca tccttcatca attgaaattg tgtcattccc atactttttc
     2161 cagcttcttt aatgttgcta tcaatatttt gaacaccgag taccgtgtta tttaaaatag
     2221 gtaataatac ataaataaat agcgctacaa ttgcaggcgt tttaccaaca ccaaaaatcg
     2281 gtatcataat agcaagtaca gctagtgttg gaatagtttg taagacacct gccacagtta
     2341 atacaatatt ggcagttcgc tttgtttttg ataataaaat gcctataggt actgcaacaa
     2401 tgatggcaag taataatgcc actatagaaa tatagaaatg ttccagtgtt ttcgacatca
     2461 actgtccacc atgttcatgt aggaactcaa tcatgattta tcatctccta tgtcacgtac
     2521 tttgacatta gtagtgtgtt gctcatgcac ttttgaggac gcagtgtctt cccccacatg
     2581 ttctgtttgc actgtatcct cactatcgcc ccaaatcgtg tcatatacaa tatcaacaac
     2641 attggcacgc gtaatcagtc ctactaaacg ctgttgatca tctacgacag gtacattcct
     2701 aacgtttctt tttaaaatcg tacgtacaga atcttgtaat ttagaatcaa tttgaacggt
     2761 ataaatatgt tgttgcatgg tgtctcgtaa acttttgtgt ccacgtatac cctgatttat
     2821 atcttcaatg tctaagaaac ctagtaaatg gttattacta tctactacaa aaatagcatc
     2881 aacacgtttt tgtctcataa tatgaacggc gtcattcagt gttgcttctg cttgtatcgt
     2941 gattggttta atcattacac cttctacagt cttgtcattg ggacggtctt gaatcagtct
     3001 attttgtcct ataaaatcac gtacaaaatc atttgcggga tgtcttaaaa tattgtctgg
     3061 cgtatcaaat tgcaccacct taccttctga cataatacaa attttgtctg ctaatttaat
     3121 cgcttcatcc atatcatgtg ttacaaagat aaacgtcttg cctaattttc gttgtaacgt
     3181 tttaactaaa tcttgtaacg tatctctcgt aataggatcc aatgcaccaa aaggttcatc
     3241 cattaaaata atatcttgtt cggccgcaag tgctcttaca acaccgatac gttgttgttg
     3301 cccacctgat atttctgctg gataacgctc taaaaatgac tccggtaaat ccacaagttt
     3361 aattaattcc tttgcacgtt tatccttttc ctctttagtc catttcaaca atttgggtac
     3421 caacacaata ttctctttaa tcgtcatatg aggcattaag ccaatttgtt gaataacata
     3481 gccaatattt ctacgcaatt cgacaggatt catactccga acatctttac catcaatttc
     3541 aatttgtcct tctgtcgctt caatcatacg gtttatcatt cttaaagcag tcgttttgcc
     3601 acttccactg gttccaataa atgcgataaa ttccccagat tgaatatcta aagagatgtc
     3661 atctactgcc tttttattac cagaataaat tttcgttaaa tgcttaatac ttaacataat
     3721 catttctcct tcctcataaa aaatatgacg aatgatacgc ataaaataaa aagaccatta
     3781 tcgcaattgt taaacaaata gagtcataag ttaaacaagt acacatcatg gtcttgatta
     3841 tgtagaattt tatatttttc atctgtaatg tgaatactta acagtaaacg gcgctatgta
     3901 tgttgaattt ctaccgatta ctttgtttgc acattcgtat attcgacatt taaaactaac
     3961 atactctata tgatgttatt aaacattatt attttatctt tggcaactaa atagataact
     4021 catatttttt aaaagcgaat ttattatcgc tcaattggtt gttttatagc aaaaaagtgt
     4081 cgaaaatgtg aaatattatt acaaaagtcg aatttttgta ataatgcaca cgcatccatt
     4141 gttacagacg gacaaccgcc catccgccac tcacaaagtc atccttatat tttaatatct
     4201 tcatcattca ttgtagacac ccttgccttt tgctaacatt ttctactccc aagcctgtaa
     4261 cggaattcca acgtcaaagt gttgcccatg acagacacac ctaaaaaaac aaccaacagc
     4321 atgagccatt ggttgtttcg tgtcatcttg tatgttgttg tataaccggt atgctttact
     4381 cattaattac ttatcatgca tccctttacc ttctagtatt tggttaatat acttttcaac
     4441 acgcttttgt cttgtttcac tgcgtttagc ttgtccaata tgatatatat atattgatgt
     4501 tgtctgcctg gcgttaattt ataaaatgaa gactctaact gtggcatttc ttcgaactta
     4561 gcttctaact ctttaggaat aacatattcc tctgttttct tcatttcaac ttttttacca
     4621 gctttttcag ctttaattgc ttcggctaga taatatttaa tttcttcggt acgtgcttga
     4681 atctctgtta aattttcaaa tcgtaactga cgagctgctt gcaccttttc agtctgttga
     4741 atgagtgtat gatatttatc ctccaaaatg gcacctttat gaaataatag tgcaacataa
     4801 tttttgaagc catggataag tactacattt ttattattca acgtgtaaca aggatgcatc
     4861 catttatacg cttcctctaa ttctgtttca ttaaagatta acgctctcaa atatttatat
     4921 tcttcttgcc attgactctc tttatttaaa aatgcttcaa cttgttcatt tgattgtttc
     4981 tcactcatag tcatcacttc cattttgttt ttcaatacac attgtattat atatcctaat
     5041 tttataaaaa actaatttta caaatgcttt tgcgttctta caaaaaatgc atttgactat
     5101 tattataata agcgtataat tgtcgcatat tattttttgt atttttggca ataacgaagg
     5161 agtatttatg aataaagaca agcaattgca caacgacaaa atcaatctat cccaattagt
     5221 cttattaggg ttaggctctt taataggatc tggttggcta tttggtgcgt gggaagcatc
     5281 atcaatagct ggaccagcag caatcatatc atgggttctt ggattcctag tcattggaac
     5341 cattgcctat aactacattg aaatcggcac aatgtttcct caatcaggtg gcatgagtaa
     5401 ctatgcccag tatacacatg gctcattatt aggctttatt gctgcttggg cgaattgggt
     5461 gtctttggtg acaataatac ctatcgaagc tgtgtcagct gttcaatata tgagttcttg
     5521 gccgtggcat tgggcgaaac caatgagata tttaatggaa aatggctcta ttagcacata
     5581 cggattgcta gctgtatatc tcatcattgt tattttttca ttattaaact attggtccgt
     5641 aaaactttta acatcattta cgagtttaat ttctgtattt aaattaggcg tacccatgtt
     5701 aaccatcatc atgttgatgc tatcaggatt cgacacttca aattacggcc attcggcaag
     5761 cacatttatg ccttacggaa gtgcaccgat ttttgctgca acaacagcat cagggattat
     5821 tttttcattc aattcattcc agacaattat taatatgggt tcagaaatta aaaatcctga
     5881 aaaaaatatc gcaagaggca tcgctatctc actgtcaatc agtgcagtgt tgtacatcat
     5941 tttacaaagt acgtttatca cttctatgcc tcaatcaatg ttacaacata gtggatggaa
     6001 tggcatcaac ttcaattcac catttgctga tttagctatc ttattaggaa ttaattggct
     6061 cgcaatttta ctatacattg aagcttttgt atcaccattc ggtactggcg tgtcatttgt
     6121 cgccgttaca ggtcgagttt tacgagcaat ggagaaaaat ggacatatcc ctaaatttct
     6181 tgggaagatg aatgaaaaat atcatatccc acgtgtagca atcatcttta atgccatcat
     6241 tagtatgatt atggttacat tatttagaga ttggggtacg ctagcagcag ttatttctac
     6301 tgcaacttta gtagcctatt taactggccc aacgacagtg attgcattaa gaaaaatggg
     6361 accaacaatg actcgtccat ttagagcaaa aattttaaaa gtaatggcac cattatcatt
     6421 tgtattagct tcattagcta tatattgggc aatgtggcca acaacggctg aagttatttt
     6481 aatcattata cttggattac caatctactt cttctatgaa tatcgtatga attggcgtaa
     6541 tacaaagaaa caaattggtg gtagcttatg gattattgta tatttaatcg tgctatcaat
     6601 actgtcattt ataggaagca aagaatttaa aggcttaaat atgattcact atccatttga
     6661 ctttatcgtt attattattg tggcacttat cttctattac atcggtacaa cgagttcatt
     6721 tgaaagcgtc tatttccgtc gcgcaacacg aatcaatacg aagatgcgtg agtcactaaa
     6781 taatgaatca aagtcatctc attaaatatt ttaaaataca gtgccatata tgatttcaac
     6841 acacacacat taaccaacca ttgatttcaa catcttggtt ggttttttat tttgaaaatc
     6901 ggttataaat aactaacata acaagatgat gatcaggc
//
LOCUS       DALAGF010000130         6668 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.130, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000130 DALAGF010000000
VERSION     DALAGF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.130"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            158..559
                     /locus_tag="O2692_002488"
     CDS             158..559
                     /locus_tag="O2692_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCZ4300090.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(679..1242)
                     /locus_tag="O2692_002489"
     CDS             complement(679..1242)
                     /locus_tag="O2692_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ4300091.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1239..2192)
                     /locus_tag="O2692_002490"
     CDS             complement(1239..2192)
                     /locus_tag="O2692_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCZ4300092.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2302..3483
                     /locus_tag="O2692_002491"
     CDS             2302..3483
                     /locus_tag="O2692_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4300093.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            3771..6188
                     /locus_tag="O2692_002492"
     CDS             3771..6188
                     /locus_tag="O2692_002492"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCZ4300094.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYK
                     EKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLAIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            6210..6521
                     /locus_tag="O2692_002493"
     CDS             6210..6521
                     /locus_tag="O2692_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCZ4300095.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
BASE COUNT         2237 a          956 c         1283 g         2192 t
ORIGIN      
        1 aattttattc aactttgaca attgaataga aagcaagttt atttacactt gtagttttat
       61 gcataagtta gcacatacaa gttttgggat tgttgggatg tttgttaata cttgtatagt
      121 agctaaatat gtgattatta attgggagat gtttagcatg aaaaaagtaa ataacgacac
      181 tgtatttgga attttgcaat tagaaacact tttgggtgac attaactcaa ttttcagcga
      241 gattgaaagc gaatacaaaa tgtctagaga agaaatttta attttactaa ctttatggca
      301 aaaaggttct atgacgctta aagaaatgga cagatttgtt gaagttaaac cgtataagcg
      361 tacgagaacg tataataatt tagttgaatt agaatggatt tacaaagagc gtcctgttga
      421 cgatgaaaga acagttatta ttcatttcaa tgaaaagtta caacaagaga aagtagagtt
      481 gttgaatttc atcagtgatg cgattgcaag tagagcaaca gcaatgcaaa atagtttaaa
      541 cgcaattatt gctgtgtaag tttaatagca taaaaagagg ttttcattaa gttgaaaacc
      601 tctttttgtt gttggcatta atttttcaaa tgttgactac tcaatcctaa attataaata
      661 gtatagcgca gcaaatgctt aagaaatttt ttctatggca caaatgaatg gagcatgatt
      721 acgttggttt aaaaattgat attgcaaaac ttgcgcatgc ttttgatcca aagtactcaa
      781 gtaatcaagc aatgcatgct tctcaatttg tccttcgcta tgaccatgat atataacaag
      841 tacaataata ccttcaattg acattaatga tagcaatgaa ttaatagctt ggattgtcgt
      901 gtcaggcttt gtcacgatag atttatcacc tttaggcaaa taacctaggt taaagatggc
      961 tgcatcaata tgacctttat gtgcatcatt tatatgatgt tcaatatttt catgtccatc
     1021 ttttattaaa gaaacatgat tgaaatcctt aactttatca cgtgtatttt ccaaagctaa
     1081 atcttgaatg tcgaaaccat aaacatgtcc ttctggtact tgttcggcta aaaataaagt
     1141 gtcattgccg ttaccgcaag ttgcgtctac aacaatactt tctggtgtta tatgttgttt
     1201 aataagtgtt tttgaaaaag ggagtatacg ttctaatttc attgcttcac cttagacttg
     1261 taacgcaagc cttgataaga atttctacgt gctaattcag catcgatgcc atttaatact
     1321 tcccatttat taacactcca cattggacct accatgatat ctattggacc atcaccggta
     1381 attcggtgaa cgatcatttc agggggaatc acttctaatt ggtccacaac taggtttgtg
     1441 tactcttctt gagtcataaa agttaataaa cctttatcgt attgttttac catcggtgta
     1501 ccttttaaca aatgaagtaa atgaatttta ataccttgta catccatttg tgcaacttct
     1561 ttggcagtag ccatcatcat gtcatagtct tcgccaggta agccattaat gatgtgtgta
     1621 catacattga tattatgctt acgtaatttt gccacaccat cataataagt tttcatatca
     1681 tgggcacgat tgattaaatc agatgttgac tgatggattg tttgtagtcc taattcaacc
     1741 cataagtatg ttcgttgatt caaatctgct aaatattcga caacatcgtc tggtagacag
     1801 tcaggacgcg taccaataga taatcccaca acacccggtt ctttaagtac aggttcgaat
     1861 ttttctttta atacttcaac cggtgcatgt gtatttgtaa atgcctgaaa ataagcaata
     1921 tattttcctt cgtgccattt ctcatgcatc ttttctttaa tttctttaaa ttgtactgcg
     1981 attgaatctg cacgattacc tgcaaagtct ccgctacctg cagcagaaca aaatgtacat
     2041 ccaccatgtg ctacagtgcc atcgcggtta ggacagtcaa acccgccatc caatgcaact
     2101 ttaaatattt tttgtccaaa tttatttttt aaatggtaat tccatgtgtg ataacgtttg
     2161 ttttcaaaag cgtattggaa atgattgccc atatgtcatt ttcctttcta taaaaaaaga
     2221 gttctaagta cagattttaa catattttaa tgttatagtg tttattatag tttgacaaaa
     2281 aagagagagg aactatgaaa tatgaatata cctaaatcag tctggtggct agtaattggc
     2341 atggcgttaa atattactgg ttccagtttt ttgtggcctt taaatacaat ttatatgaaa
     2401 caagaacttg gaaaaagttt aactgttgct ggtttagtgc taatgataaa ttcatttggc
     2461 atggttattg gaaacttatt aggtggttca ctatttgata aattaggtgg atacaagacg
     2521 attttaattg gaactttcac ttgtctttgt agtacaacgc tacttaattt ctttcacggg
     2581 tggccttggt atgctgtatg gcttgtaatg ttagggtttg gtggcggaat gattattcct
     2641 gcgatatacg ctatggctgg agcagtgtgg ccaaatggcg gaagacaaac gtttaatgcg
     2701 atatacttag cgcaaaatat tggtgtggct gtcggtgctg caatgggcgg ctttgtcgca
     2761 gaatttagct ttaactatat ctttttagcc aatcttatta tgtatgttgt gtttgcgctt
     2821 gtcgcggtaa cgcaatttaa tattgaaatt aatgcgaaag ttaaatatcc aactcattta
     2881 gatattactg gtaaaaagaa taaagcaaga tttatttcat tagtactaat ttgtgcaatg
     2941 tttgcaattt gttgggttgc atatattcaa tgggagtcta caatcgcttc atttacacaa
     3001 tctattaata tttcaatggc acaatatagt gttttatgga caattaacgg aataatgatt
     3061 ttagtagcac aaccattaat taaaccgatt ctctatctgt taaaaggaaa cttaaagaag
     3121 caaatgtttg tcggcatcat catttttatg ttgtcgttct ttgtcacgag ttttgccgaa
     3181 aactttacaa tatttgttgt cggtatgatt attttaactt ttggagaaat gtttgtatgg
     3241 ccagcagttc caactatagc caatcagtta gcgccagatg gtaagcaagg acagtaccaa
     3301 ggttttgtga attcagctgc tacagtagga aaagcatttg gtccatttct tggtggtgta
     3361 ttagttgatg cgtttaatat gcgcatgatg tttatcggta tgatgctact acttgtattt
     3421 gcattaatat tattaatggt tttcaaggag aataatacgc aacctaaaaa aatagatgca
     3481 taatgagtaa atagaattaa cgttatagac ttgaaataaa tgtcgttata acataatatt
     3541 aatttgtata atttaatttc gtttggagct tttctacaga aagctagtga tgctgagagc
     3601 tagtgttaag gactaaatgt aaatcgtatt aattttaaat tgaatgaatg acatctctta
     3661 ctattaaaat gagtgcacaa tttttgtgaa atagggtggt aacgcggcaa atgtcgtccc
     3721 tatgtaaata gaatagttag aggtgtcttt tttattgaat aggaggaaat gtgttgaatt
     3781 ataaccacaa tcaaattgaa aagaaatggc aagactattg ggacgaaaat aaaacattta
     3841 aaacaaatga taacttaggt caaaagaaat tttatgcttt agacatgttt ccatatccat
     3901 caggtgctgg tttacatgtt ggacatcctg agggctatac agcaacagat atcatttcaa
     3961 gatataaaag aatgcaagga tataatgtat tacatccgat ggggtgggat gcattcggat
     4021 taccagcaga gcaatatgct ttagacactg gcaacgaccc acgtgaattt acaaagaaaa
     4081 atatccaaac ttttaaacga caaattaaag aattagggtt cagttatgat tgggatcgtg
     4141 aagttaatac aacagatcca gaatactata aatggacaca gtggattttc atacagttat
     4201 ataacaaagg tttagcatac gttgatgaag ttgcagttaa ctggtgtcca gcattaggca
     4261 ctgttttatc taacgaagaa gtgattgatg gtgtctctga acgtggtgga catccagttt
     4321 atcgtaagcc gatgaaacaa tgggtactta aaatcacaga atatgcagat caattattag
     4381 cagatttaga tgatttagat tggcctgagt ctttaaaaga tatgcagcgc aattggattg
     4441 gacgttctga aggggccaaa gtttcatttg atgtagataa tacggaagga aaagtagaag
     4501 tatttacgac tagaccagat acaatctatg gtgcatcatt cttagtctta agtcctgaac
     4561 atgcattagt taattcaatt acaacagatg aatataaaga aaaagtaaaa gcttatcaaa
     4621 cagaagcttc taaaaagtca gatttagaac gtacagattt agcaaaagat aaatcaggtg
     4681 tatttactgg tgcatatgca actaatcctt tatctggtga aaaagtacaa atttggattg
     4741 ctgattatgt attatcaaca tatggtactg gagcaattat ggcagtacca gcgcatgatg
     4801 acagagatta tgaatttgct aaaaagtttg atttgccaat cattgaagtc atcgaaggtg
     4861 gaaatgttga agaagcagca tacactggtg aaggtaaaca tattaattct ggtgaacttg
     4921 atggtttaga aaatgaagcg gcaattacta aagctattca attattagag caaaaaggtg
     4981 ctggcgaaaa gaaagttaat tacaaattaa gagattggtt attcagtcgt cagcgttatt
     5041 ggggcgaacc aattcctgtc attcattggg aagatggaac aatgacaact gttcctgaag
     5101 aagagctacc attgttgtta cctgaaacag atgaaatcaa gccatcaggg actggtgagt
     5161 ctccactagc taatattgat tcatttgtaa atgttgtaga tgaaaaaaca ggtatgaaag
     5221 gacgtcgtga aacaaataca atgccacaat gggcaggtag ttgttggtat tatttacgtt
     5281 acatcgatcc taaaaatgaa aatatgttag cagatcctga aaaattaaaa cattggttac
     5341 ctgttgattt atatatcggt ggagtagaac atgcggttct tcacttatta tatgcaagat
     5401 tttggcataa agtcctttat gatttggcta tcgtacctac taaagaacct ttccaaaaat
     5461 tatttaacca aggtatgatt ttaggagaag gtaatgagaa gatgagtaaa tctaaaggaa
     5521 atgtaatcaa tcctgatgat atagtacagt ctcatggtgc agatactttg cgtctttacg
     5581 aaatgtttat gggaccttta gatgctgcaa ttgcatggag tgaaaaagga ttagatgggt
     5641 ctcgtcgatt cttagatcgc gtatggcgtt taatggtaaa tgaagatggg acattgagtt
     5701 caaaaattgt aactacaaat aataaatctt tagataaagt ttataaccaa actgttaaaa
     5761 aggtaacaga agactttgaa acattaggat ttaatactgc tattagtcaa ttaatggtat
     5821 ttattaatga gtgttataaa gttgatgaag tttataaacc ttacattgaa ggcttcgtta
     5881 aaatgttagc acctattgca ccacatatcg gtgaagaatt atggtcaaaa ttaggacatg
     5941 aagagtctat tacgtaccaa ccttggccaa cttatgacga agcactactt gtagatgatg
     6001 aagtagaaat cgttgttcaa gtgaatggta aattgagagc taaaattaaa attgctaaag
     6061 atacatcaaa agaagaaatg caagaaattg ccttatctaa tgacaatgtt aaagcgagta
     6121 ttgaaggtaa agacatcatg aaagtcatcg ctgttcctca aaaattagtc aatattgtag
     6181 ctaaataatg ttttaaggag gactttgaaa tgaagtcaat tactacagat gaattaaaaa
     6241 ataaactttt agaatctaaa ccagttcaaa ttgttgatgt tcgtactgat gaagaaacag
     6301 caatgggata tattcctaat gcaaagttaa ttccaatgga taccattccg gataatttaa
     6361 attcatttaa taaaaatgaa atatattata ttgtatgtgc tggtggagtt cgaagcgcta
     6421 aagttgtaga atatttagag gcaaatggca ttgatgccgt aaatgtcgaa ggcggcatgc
     6481 acgcatgggg cgatgaaggt ttggaaataa aaagtattta aagtagtgac ataatttaaa
     6541 ataatattac atttgtaatg acaccaagta acgtttcggt tgcttggtgt tttttggtat
     6601 gaattacttt ctgttacaaa acaatctaaa gcgttcttgt tatgttttat taagatttta
     6661 attacaaa
//
LOCUS       DALAGF010000131         6624 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.131, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000131 DALAGF010000000
VERSION     DALAGF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.131"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            188..1636
                     /locus_tag="O2692_002494"
     CDS             188..1636
                     /locus_tag="O2692_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCZ4300096.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            complement(1717..3054)
                     /locus_tag="O2692_002495"
     CDS             complement(1717..3054)
                     /locus_tag="O2692_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HCZ4300097.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            3312..3728
                     /locus_tag="O2692_002496"
     CDS             3312..3728
                     /locus_tag="O2692_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300098.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPYILEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            3851..4741
                     /locus_tag="O2692_002497"
     CDS             3851..4741
                     /locus_tag="O2692_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HCZ4300099.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVESKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDNLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNAELIASFSRALASDLRADQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(4934..6430)
                     /gene="mqo"
                     /locus_tag="O2692_002498"
     CDS             complement(4934..6430)
                     /gene="mqo"
                     /locus_tag="O2692_002498"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCZ4300100.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
BASE COUNT         2021 a         1166 c         1120 g         2317 t
ORIGIN      
        1 aatatctgtt ataatcagtt ttatcattta aaaaatctat ggatgttatc aagacaagtc
       61 attaatacgt aacataaagt tgtgattttt cagaattttt agacaaaata ttgattattt
      121 gtgctatgat ataacatatt aaaaaacacc gtatagaatt gtttatgaat gtttgggagg
      181 aaagtttatg ggaagttttt tcaataaaat agcacgaaaa gaggatccgg ctatctatca
      241 aaataaagat ggtcatttaa agcgtacact tcgggtgcgt gatttcttag ctttaggtgt
      301 aggaacaatt gtatcgacat ctatctttac gctacctggc attgttgctg cagaacatgc
      361 aggaccggcc gttgcgttat cattcttact cgctgctatt gttgctggtt tagttgcatt
      421 tacttatgca gaaatggctg ccgctatgcc atttgcaggt tcagcctatt cttgggtcaa
      481 tgtattattt ggtgaatttt ttggatgggt tgccggttgg gctctattag ctgaatattt
      541 tatcgccgta gcctttgttg catcaggatt ctcagcgaat ttacgcggac ttgtgaaacc
      601 aattggcatc gaattacctg cagcattatc aaatccattt ggtacaaatg gcggttttat
      661 cgatattatt gctgctatcg ttattttatt aactgcatta ttactatcac gtggtatgtc
      721 ggaagcagct cgtatggaaa atattttagt tattttaaaa gtattagcta ttattttatt
      781 tgtcatcgta ggtttaacag caataaatgt tagtaactat gtgccattta ttccagaaca
      841 caaagtaact gctacaggtg actttggtgg atggcaaggc atatatgctg gtgtttcaat
      901 gattttctta gcgtatatcg gtttcgattc tatcgcagca aactcagcag aagcacttga
      961 tcctcaaaag acaatgccta gaggtattct tggttcttta agcgttgcta tcgtattatt
     1021 tattgctgta gcacttgtgt tagttggtat gttccattac tcacaatacg caaacaatgc
     1081 tgaacctgtt ggttgggctt tacgtcaaag tggtcatggt gttgtagcag ctattgttca
     1141 agctatctct gttatcggta tgtttacagc attaattggt atgatgttag caggctcacg
     1201 tttactttat tcatttggac gtgacggctt attaccttca tggttaagcc acttaaacga
     1261 caaacattta cctaatcgcg cactagttat acttactatt attggtgttt taattggttc
     1321 tatgttccca ttcgcatttt tagcacaatt aatttcagca ggtacacttg ttgcatttat
     1381 gttcgtttca ttagcaatgt atcgtttgag aaagcgtgaa ggtaaagatc taccaattcc
     1441 tgcatttaaa ttacctttat atcctgtgtt accagcaatt acatttgttt tagtattgct
     1501 agtattctgg ggattaggat tcgaagcgaa attatacact ttaatttggt ttattgttgg
     1561 tatcatccta tatttaagtt atggacttag acattctaag aaaaatgacg tagctgaata
     1621 ccacccacca aaataaaaaa taaaaaaaat catttcccat ctgattaaca tccagatgtg
     1681 gaaatgattt tttattttat attaagataa tcgctattac caaccttgtc cagatatgtc
     1741 atattgatct aagttacctg cttccaaagc agtcagtgca tcttctatag tgtttaacgc
     1801 cgtgtctaat tgctcatatg ttattaccaa tggcggttgg aatcgcaaca catttcctgc
     1861 tacagctata ataactacgc catgctcaaa gcagtaatta caaattttaa gtgccgcact
     1921 ggcatcacgt gttttgagtt ttttgtcgga aacaatatca ataccaatgc tcagaccttt
     1981 acctctaaca tcgcctacac tattgtattt agatacccat tgatccattc gtttcctaac
     2041 atattcccct ttttccgcac tagcctgaag aagcgactga tcttcaatca tttgaattgt
     2101 ggctaatgca gcttcacaac taactggatt agcacctgtt gtaaataaat gtgctggtgc
     2161 ttctaaacaa ttcataatct ctttgcgtcc aacaattgct gacataggca taccacctgc
     2221 taaggatttt ccgaaagtga ttaaatcagg cgtaaaatta aaatgcgaga ctgaactcca
     2281 tgtacctgtt ctcccaaaac cttgttgaat atcatcgaca gcgattaaaa taccatgttc
     2341 acgacaaatc ttttctaacg cttcaaaata ccctggaact ggttctaaaa gtccaccatc
     2401 gccttgtatc gtttcaataa caatacatgc tacttcgtca gcaggtacat acttcgcaaa
     2461 catttctttt aagggtgcta aatattcttc tactgaatta gcttgtggct gctcgtacat
     2521 accacgatat ttatctggaa acggaatatg ataaaaacca ttcaataacg gaccataatg
     2581 tttgcgcata tttaaactaa tagctgacat agacaatgag ccaaaagttg aaccatgata
     2641 tgcattagtg aaactaatga tataaggacg ccctgtatat gctctggcaa atttaatgat
     2701 gccatcattt gcgtctgatc cggttaatcc gaaggtcact cttttttcaa aatctccagg
     2761 tgcaatctca caaagcttct tagctaaacg taccaacggt tcatgataca tatatgctgg
     2821 tgtataatga ataaatttat caacttgtgc ctttatcgct tctgttactt ctctaggtgc
     2881 atgacctacg ttttgagaac tcgcactcga taacaaatcg atatatgtct tcccctcaat
     2941 atcaaccaat gttgctccat atccatgatc aatcactaac ggataatatt taatacgtcc
     3001 tgatttcgca aaataatgtt catcctcttg aattaattga tgtgctttac tcatatacat
     3061 tcccctccca ttgacagtat taatgtgcaa gcaaagtggc ctctcttaaa tcgattataa
     3121 aatccgattt aaaatcctgc tcaaataccg tcatgcctct atgaatacta tccaatatac
     3181 taataaaatt cagatttgta aatatattct gataattaag aattttatat caatctactt
     3241 tgtttatgcg cataattttg ttcgattagg gaattgttta ctatattcca tttgactctg
     3301 aggtgatacg aatgcgttta aaagtattgt tccatttcat tgctgctatt ttcatatcat
     3361 tcatgttgct atggatgacc atgttgtttg acttaataag caatcaatca catttaaaag
     3421 ctttattatt gaatttagat tttctaattc cttctgacaa tacaccatat attctagaaa
     3481 taatatgtca cttactaatt ggtagcgtca tttattttgt atttgtgttg ctgtttcata
     3541 cttctaaaag gctttactat ctatgttata tccctttatt tttcttattt atagcattgt
     3601 atcccttttt agtatttatt gcgcaacgtc caatttttca atttagtgtg acagaattaa
     3661 tcggttggat tattacacat attttcttta tgtcattaat ggcgttagtc atacctagaa
     3721 taaaataata tttttaatta aactgacaat tatttttcac attttataca cttttcaagg
     3781 tttatgactt gagaaatgtt ctcattttta tcatactgga ggtgtattga aaagttaagg
     3841 agtggaaagt atgaataaag agcaattaga aaaaatgaaa aatggaaaag gctttattgc
     3901 cgcattagac caaagtggtg gtagtacacc aaaagcactt aaagaatatg gtgtaaacga
     3961 agatcaatat agcaatgaag acgaaatgtt ccaacttgtt cacgatatgc gtacacgtgt
     4021 ggtaacttca ccttcattct caccagataa aattttgggt gctattcttt tcgaacaaac
     4081 aatggatcgc gaagtagaaa gcaaatacac tgcagattac ttagctgata aaggtgttgt
     4141 tccgttctta aaagtagaca aaggtcttgc tgaagagcaa aatggtgttc aattaatgaa
     4201 accaatcgac aacttagaca atttattaga ccgtgcaaac gaacgtcaca tttttggtac
     4261 aaaaatgcgt tctaacattt tagaattaaa tgagcaaggt atcaaagacg ttgttgaaca
     4321 acaatttgaa gttgctaaac aaattattgc taaaggttta gttccaatta tcgaaccaga
     4381 agttaatatt aatgcaaaag acaaagctga aattgaaaaa gtattaaaag ctgaacttaa
     4441 aaaaggttta gatagcttaa atgctgatca attagtaatg ttgaaattaa caattcctac
     4501 tgaaccaaac ttatacaaag agttagctga acatcctaat gttgttcgtg tcgttgtatt
     4561 atcaggtggt tacagcagag aaaaagcaaa tgaattactt aaagataacg ctgaacttat
     4621 cgcaagtttc tcacgtgcat tagctagtga tttaagagct gatcaatcta aagaagaatt
     4681 cgataaagct ttaggtgatg ctgtagaatc aatctacgac gcatctgtaa acaaaaacta
     4741 atttatacat taaactattt tttgatataa actactaaaa aggactcata catgtgcttt
     4801 aaaggcattg tgtatgagtc ttttttgatt aaagttgatt ttgccgtatg tattagatta
     4861 cgtaaaaaaa tcggacagct gaaaaatctt taagatttct cagttgtccg attttaaata
     4921 tagctattag aatttagttt tcgtagtaac ctaattctaa gtcttttgaa gtttgtttac
     4981 ggatttttct cattaatttt tcgtcttcaa ttaatgattc accgtatgat ggaatcattt
     5041 tcttaatttt aggtgcccat tcagttttgt attcagggaa gttacgttct aatacttcta
     5101 acgcaactga aactgaagtt gaagcccctg gtgattcacc taataatgca attacagtgt
     5161 ggtcttgtga gttaaccact tctgtaccga attggatgaa tcctttaccg tgttcaggtg
     5221 tatctttgat aacttgtaca cgtttaccag cagtgtataa ttgccaatct tcattacgtg
     5281 cttctggata gaaagtacgt aagtggttca tacaaccttc ttttgtcata attacttggt
     5341 caaatgagta tttaattaaa ggtaagtttt taactgctgc tgctaataaa gttgtaatgt
     5401 tgtatgtttt aacagacttg aataaatcta agttagaacc atttttcaag aatttaggtc
     5461 caacattagc aaatggtcca aataataatg ttctttgacc atcaatgtaa cgcgtatcta
     5521 aatgaggtac agtcattggt ggtgtaccag gtggctcttt accataaact ttggcatcgt
     5581 gttgttcaat aacttgtggg tttgtacaag ctaagaattg accactgata gggaatccac
     5641 ccaaatgttt actttcaggg atacctgttt tttgtaataa tggaattgct ccaccgccag
     5701 caccgatgaa tacgtagtca gttacttgtt tgaatttctc accagttagg cgatttttaa
     5761 cagtaacttc ccattgacca tttgataatt gttcaaaatc aacaacttca tggttaaatt
     5821 gcactgtagc atttggatgt gcttcaatgc ttttagccat tttacgtgtt aattcaccga
     5881 agtttacatc tgtaccttcg tcaattttac ttgccgccat gataccaggg ttatcttcac
     5941 ggcctttcat catcaatgga atccattttt tcattacttc gatgtcttca gtatattcga
     6001 tattatcgaa catagggaaa gctttcatcg cttcgtaacg atcttttaag aatttaacat
     6061 tgtttttacc tctaacataa ctgatgtgtg gtaatggatt gataaattct cttgggttct
     6121 cgatgctacc gcttttcact aagtgacccc agaattgttt tgaaatctca aactcttcgt
     6181 taatcacttt cgctttttcg atgtcgatag aaccatcagg ttgtaaaact gtgtagttca
     6241 actcacataa tgctgcatga cccgtaccag cattatttct ttcgtttgaa ctttcgattg
     6301 caggacgatc caagcgttcg taaacgtgga tattccagtc tggctcaatt tcttttaaca
     6361 ttgaaccaaa tgttgtgcta agtacaccgg ctccaattaa aacgatgtct ttactattag
     6421 acttagccat tggtttcacc tctccaaaaa ttgtaaatgt gtatcatcaa tatgaaagtt
     6481 acataaaact gacatatttc tttaaaatat caacgccatt gataacttcc tgttttaatt
     6541 gatacgctgt aacaaaatac tatagttagt gcttacatgt atatgttaaa gcaagcagtg
     6601 gtaaatgtaa attataatta ttca
//
LOCUS       DALAGF010000132         6618 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.132, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000132 DALAGF010000000
VERSION     DALAGF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.132"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            54..1436
                     /locus_tag="O2692_002499"
     CDS             54..1436
                     /locus_tag="O2692_002499"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCZ4300101.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
     gene            complement(1490..2065)
                     /locus_tag="O2692_002500"
     CDS             complement(1490..2065)
                     /locus_tag="O2692_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300102.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            2396..3250
                     /locus_tag="O2692_002501"
     CDS             2396..3250
                     /locus_tag="O2692_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300103.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEISNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
     gene            complement(3393..3926)
                     /locus_tag="O2692_002502"
     CDS             complement(3393..3926)
                     /locus_tag="O2692_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCZ4300104.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            4065..4253
                     /locus_tag="O2692_002503"
     CDS             4065..4253
                     /locus_tag="O2692_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HCZ4300105.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            complement(4366..4968)
                     /locus_tag="O2692_002504"
     CDS             complement(4366..4968)
                     /locus_tag="O2692_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCZ4300106.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVFLDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(4965..6056)
                     /locus_tag="O2692_002505"
     CDS             complement(4965..6056)
                     /locus_tag="O2692_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCZ4300107.1"
                     /translation="MKFLKDTSIAEISSIVYLIFPIAGIFFNEVYGPKWLYIISVIVF
                     SLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKN
                     NVKKTATNLFILTMIICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKE
                     IAEKNRHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDADVEKVKAELL
                     AINKLSRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTK
                     QSMLVMITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQ
                     RVQHLNGTLAVDSTNGTKIIIEISTGGIA"
     gene            complement(6060..>6618)
                     /locus_tag="O2692_002506"
     CDS             complement(6060..>6618)
                     /locus_tag="O2692_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4300108.1"
                     /translation="YSLLSFSLLTFPLDIINEKQNEWRQRLMVTPFTFTSYYISKVVK
                     TMLQFAIAILVIFMVGHFYKGVAMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQK
                     TSALANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLIS
                     FAIILLYALGSIIAVYCISHFKRAE"
BASE COUNT         2346 a         1038 c          893 g         2341 t
ORIGIN      
        1 acttacaaaa ctgaatacaa ttttctgatt ttttagaaag gttggcatgc actatgaaag
       61 tttccaaatt cggtggtagc tccgtctcca atgctactca aattaaaaag gttttaaata
      121 ttgttaattc tgatccagag cgaaagattg ttatcgtttc tgctccaggt aaaagacatg
      181 ataatgatat taaaacaact gatttgttaa tcagattata tgaaaaggtc attaatcatc
      241 ttgattatca tgataaaaaa agagaaatta ttcagcgtta tgatgatatt gtaaaagaat
      301 tgcaaatgga tgaaagtatt ttacggacga tagatgtgac tttggaacat tatattaatc
      361 aattaaaaaa tgaaccaaag agactattag atgcattact ttcttgtggt gaagatttta
      421 atgcgcaatt gatagcttta tataataata gtcaaggtat accaacaaaa tatatatcac
      481 cgaaggaagc gggtattttt gttactgacg tcccacaaga agcacaaata ttagatagtg
      541 cttactctga aattaaaaaa ttaagagatt atgatgaaaa actaattatc ccaggatttt
      601 ttggcgtatc tcatgaagga tatatagtta catttccacg tggcggatca gatataactg
      661 gcgccatcat atcaagtggt gttagagcca caatttatga aaatttcacc gatgtgtctg
      721 gtatttataa agctaatcca aatatcatca aagatcctga attaatcgaa gaaatcactt
      781 atcgagaaat gcgtgaatta tcttatgctg gttttagtgt tttccatgat gaagccttac
      841 aaccacttta taaagatcgc atccctgtgg tgattaaaaa tacgaaccgc cctcaagata
      901 agggtacatt tatagtgcat gatagagaaa taaatgcgaa aaatgtgatt agcggtataa
      961 gttgtgataa agattttacc gttattaata tcaaaaagta tttaatgaat agacaagtcg
     1021 gctttactag aaagatatta ggtgtcttag aggataataa tatttcattt gatcatatgc
     1081 cttcaggtat tgatactatc agtatcgtta tgcgttcaaa acaaattcaa aataaagaaa
     1141 caaaagtatt aaatgaaatt cgtcaaaaat gtgatgttga tgagctaagt attgagcatg
     1201 atttagcaat attaatgatt gtcggcgaag gtatgaataa agtcatcggt actgcaaata
     1261 aaattacaca tgccttagct gaatcaaaaa ttaacttaaa aatgattaac caaggtgctt
     1321 cggaaatctc gatgatgttt ggaattcatg tagatgatgc tgaaaaagct gtattatcta
     1381 catatgagtt ttgctatcac ggggttgcat taaaaaactt gtgtgataaa aactaatata
     1441 aaagcaattc gtaacacatg tattcaactg cgttatgaat tgctttttct taattattta
     1501 ttgttctctg gattgcatcc ttaatacgtt gctcacctac tacaaatttt aaaataaaaa
     1561 attgatagta gcttaatgca tctatgactc gtttataatt ggaataatga tgatactgat
     1621 ggtgttgcat tagatcatac attagaacga tctccggttt aatataatcc acatcccaat
     1681 ttaaactatg gaagtatatg ttactcttag gtatacgtat attcggatcc aatctaaaaa
     1741 tccattcatc gtttactaca tcataaacga aaatactcat tattgttgta tcatcatatt
     1801 gcacataaac ttttgcaatt tttgaaagtg ataaatcttc tttagctatt gcttgatctt
     1861 gcttatctat gtatagtaac gcatatgatt gcggaataac tttcacaata tcacttaaac
     1921 gcttacgttc cacgcatatt tcaacaatag aaggtaattt taattcatca tttaagaaaa
     1981 gctgcatggc aacttcaccg tgaaatttaa ataattgcga tgatgttttc attaaattgt
     2041 ccactaattg ttctttagca ttcatttcat tcactctcaa atacacctcc taagcaagcg
     2101 ctttcacaaa tatcattata cattttaaaa ttaatgaatt caatcttcaa aatactatga
     2161 gtgacctaat tctgtcattt aaatgaccgt tataacataa attcgacata aacttgaatt
     2221 cacgaatctt tcacgtcata ttcattgaac atcaatatct gtcagtttat aatttgatta
     2281 taacaatgat gaactaaaat caactaataa aaatgtggga aattgaataa caattaatct
     2341 ttattaatta ttattgtata caaagttaaa gaagtcaaaa aggattggtg aaaaaatgtc
     2401 tttttatgtt gtacttatca ttatcattgt agcattaatt ggtatattag ttttaaatca
     2461 aaggtatagt aatagtaaaa tagataccga agtttatgca agaaagcaac ttattaaaaa
     2521 gaacaaagca ttaagtgcag aaaatgctga attaagaagt caaatgctga gttctaataa
     2581 tgatgttggc caccatgcat acaaaaatgc taaaagagaa ttacgcaaga ttttagatag
     2641 ctatctcgaa aatggtaaat taaaatatta cgatattatc gttacaagca atttagctac
     2701 taaacatccg tttttcgagt atgcacgttc atttgatttt atcattgttt cagatatcgg
     2761 tttaataaac gttgatgtaa aaagctgggg tgaaaaaaca ttttatcact ttgatgtacc
     2821 tgatgagcat gatacagaaa taagcaatag taatattgaa aaagttgtcg gtcattacat
     2881 tagccagcaa tatcatgatc agtttaattc atcaagaaag tctatctata cttttacaga
     2941 aacagttcaa ccaaatcgtg ttatttatga tttttatgat tatgatccgt atcaattagc
     3001 agcaaataat gcgaaagcat taaaggatca tattgaacaa aactttaact ttaaagtcca
     3061 aagtaccggt gttatttact ttagtgatgg tacagttaat atcattcaag gttctgaaga
     3121 aagagataaa tatgttgata cagtttcaac aaaatcttcg ttacgacgta ttattagcga
     3181 agccatagag ctatctaaac accctttaaa taaagaacag gttgatcaaa tcacagctat
     3241 attcaagtaa aatcaaaggc ataataatcc attacgctcg atacgcacta tggttattat
     3301 gcctttaaat tatttctata tataaattta tggtaataat aaatcgatgc ttcaagtata
     3361 ttttacttta atatatataa tcacctattt aattatttac tccaaatatt taatttctgt
     3421 tgtttagctt tattttgtgc ttctataaat acatttctat atttgccatt tggtgaaaaa
     3481 tacttctctc tagcaagtcc cttttccact aattccttat tgtacatacg atctttactt
     3541 atccatacat acgccaatgt tctaccatag cgatcttgtt tttctttatc atattctaaa
     3601 taaacatctt gatttgttaa tgtcttctta ctataatttg atgcttcttt gccaaatggt
     3661 tgtacaggcg tattcggttt caccgtttct ggcgtatcaa cccctataag cctaacttta
     3721 atttctttac cattttgatt tgcaataaat gtatcaccat ccacaactct ttgaacatgt
     3781 actttatctt taccatttaa atcttgtaca gtttcatgat tcgtcccctt tttgaaagga
     3841 cccgtatgat tcataaattg aaatgctaat acacctacaa taatgatggc taccacaatc
     3901 atagcaagcg atttatttga cttcatattg ccacctactt ctcttttttc attccgatta
     3961 atcatataaa agttaagaaa tatcgtcaat agccatatct acatatttca atattttgga
     4021 aagctatagc atgatataat gttattgtta aggagtgaaa ataaatgaac atttcaaatg
     4081 aaaatacagt cacattaatt gcagtaatta tcgcaattat cattggtatt ttcctacaaa
     4141 tatttttcaa attaccatta atagtgacgg cagtactatc tattttattg ggtatctttg
     4201 taggctttat cgtttacttg attgtttcgt tttataataa gcgaaaaaat tagttaacaa
     4261 atctaaattg cgagtaccaa aataaaagta ttgagtaaga ttttgattat tgactcactt
     4321 tccaagtctc ttaaatcaaa acactcaata cttttttgtt tgtatttaga tccagccttt
     4381 ttcatttgcc tttttccaag catcaaaacg attatctgca aataatttat caattataac
     4441 agatgtataa tttctaactg ttccatctgt caaaaataat ttttcactta tttctttact
     4501 acttaaacca ttgccaattt cccttaatac aatttgttct ttgggcgtta atgggttttt
     4561 atctacaaaa aatgaagtca tcaatgtggc gctatattct ttctctccgt tatttacttt
     4621 attaatggtt tccaccaatt cttctataga acgttctttt aaaacatatg catccacatc
     4681 attcacaact gctttttcaa agtatcccgg tcttttaaaa gttgttacaa taatcacttt
     4741 aatattcaaa tgcttttttc taatttccgc taaaacttca agtccagtca tgcctggcat
     4801 ttctatatct aaaaaaacaa cgttaggatt atattcttca ataagtttca ttgcatcgag
     4861 accattatca gtatctgcta aaatttcaaa atcaccatgt agtttaatta attgaaccat
     4921 tgcctgtcgt aacatatttt gatcttctgc aataattaaa gatgtcatgc tattcctcct
     4981 gttgagattt caatgattat tttagttcca tttgttgagt cgactgctaa agttccattt
     5041 aaatgttgta cacgctgtga aatacttttc acctcacaat cactatcgat acctttgcca
     5101 tcatcttcaa tcataagcag taatttatga ttgtttacag tttttaattt accatgaact
     5161 tttgaagcat ttgcatgttt aataacatta tttatcgctt cacgcgtaat cataactaac
     5221 atagattgtt tagtaggact taatacttgc gctaattctt tattttcaaa tgtaaaatca
     5281 atatcagcat cttttaaaac tttacgtata ctatcaatct cttcaataaa tgatggtaat
     5341 tttacatcat caataatttc tcgaactttg ttcaatgatt cacgagataa tttattaatt
     5401 gctaataatt cagcttttac tttttctaca tcagcatcta ttagtttata agctaattct
     5461 gattttaatg ataaacttgc aaacacatgc cctaacgtat catgtaagtc ttgaccaatt
     5521 ctatgtcgct cttgttcagc aattaatgta ttaatatgtc tatttttttc tgctatttct
     5581 ttttgatatt cacggttttt cattttttta aaattatcta gcattattaa cgatataacg
     5641 acataataaa ccatcattgc aacaaaatag ttgttataca ataagtacgt tattattgta
     5701 catataatca ttgttagtat gaaaagatta gttgccgttt ttttaacatt atttttaaaa
     5761 gtaaatggaa cggcaaaagc actataaaag aaaaacaaac ttagcattgg atgtacactg
     5821 aaaacaaaat aacaaataat aaaataatga ataatcaaca aaatgtaaaa cattaatgta
     5881 ttaagtctat tatttactat aactaatata agatacgaca acgaaaagac aatgactgat
     5941 ataatataca accatttggg accatatact tcattaaaaa atataccggc aataggaaaa
     6001 atcagataaa ctatagacga tatttcagca attgaagtat cttttaaaaa tttcatattt
     6061 tattccgccc ttttaaaatg actaatacaa tatactgcta ttatactccc taaagcatac
     6121 aagagtatta tagcaaatga tattaaattg atatgatgat ttgaagcaat atctacacct
     6181 agtttacgca aatgatagct cggtaaaaca tgagcaacat gttgaagcca atttggaaat
     6241 gtgtttatcg gaaaccacaa tccacctaat actgctaaac caattgttac gatattagct
     6301 aaagcacttg ttttttgaat atcatttaac aaagaaaata atatgccaaa agttattaat
     6361 agagacgcac ctaaccataa aaatattcct gactctaacc attgaactgc actcattgca
     6421 acacctttat aaaaatgtcc aaccataaaa ataactaata tcgctattgc aaattgcagc
     6481 atagttttca ctacttttga aatataataa ctagtaaaag taaatggtgt taccattaat
     6541 ctttggcgcc attcattttg tttttcatta ataatatcta atggaaaagt tagtaaacta
     6601 aaacttaaca aactataa
//
LOCUS       DALAGF010000133         6233 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.133, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000133 DALAGF010000000
VERSION     DALAGF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.133"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            1..2124
                     /locus_tag="O2692_002507"
                     /pseudo
     CDS             1..2124
                     /locus_tag="O2692_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic dehydratase"
     gene            2117..3361
                     /locus_tag="O2692_002508"
     CDS             2117..3361
                     /locus_tag="O2692_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HCZ4300109.1"
                     /translation="MIDMNLILKKKFQEISEVDDFISKASTETDYFEPSTLSHGIPGI
                     ILFLDAYQKVFDINTEQIVHKYIMKLAPYLQSGQYNNSLFGGLSGIAFSMDLASQNGK
                     NYQNILNNIDGHIVNEIENKFDQILQEPLNPLNYDTVSGLAGIGRYLLNRVDENEFNV
                     KALKSILVYFKDIQYSKNSWVVPQESQFLESDKNYFTEGNINLGLAHGVLGPMSLFAL
                     CVIKGITIENHQHILKDMYKFIMDEKFCNHERWLQRYDLISERNHFNFIRNGWCYGNT
                     GVMTTLFLIGQALQDDEIIKMSKKVMLQVVNDKDENLISPTICHGLSSQILMLTIMNL
                     NFELNEVSDYITVLINKLISHYKEDYLVNFIDINENKQDVFKSRKVGLLEGELGVYLT
                     LMTLKNTKILNEKNWTNAFLIS"
     gene            3377..3895
                     /locus_tag="O2692_002509"
     CDS             3377..3895
                     /locus_tag="O2692_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HCZ4300110.1"
                     /translation="MGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFI
                     NGEILKQFCDNYYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTI
                     CHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNV
                     VAPEPYKVLEFI"
     gene            3905..5278
                     /locus_tag="O2692_002510"
     CDS             3905..5278
                     /locus_tag="O2692_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HCZ4300111.1"
                     /translation="MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFN
                     KLVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDR
                     TSNESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKN
                     LVPLNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGS
                     KKTELLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINY
                     AKKKKSIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSN
                     LSEFSNFGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGT
                     SLATPKVSGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALN
                     VANQKAS"
     gene            5301..5993
                     /locus_tag="O2692_002511"
     CDS             5301..5993
                     /locus_tag="O2692_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="HCZ4300112.1"
                     /translation="MAKLVTENISKRFKNQDVLKHINITLENNEVYGLLGINGAGKTT
                     LMKIICGILQQDSGEIKLDNRPMTRNDLHKVGSLIETPATYNHLSAQDNLKIVCLNES
                     VDFSEINSVLSLVNLNVDKKKKVKDFSLGMKQRLGIAMALIKKPEILVLDEPSNGLDP
                     YGIQELRELLKLLTEQGTSIIISSHILSEIQVLADHIGIIHEGELKYQQRNNKDENLE
                     EIFFKITKGDYK"
     gene            5990..>6233
                     /locus_tag="O2692_002512"
     CDS             5990..>6233
                     /locus_tag="O2692_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HCZ4300113.1"
                     /translation="MIHLKIEGIKFKNSFSMYVLLISPLVFLCFAIFTVLFAKSNTGT
                     ANSVSPYITLLFNIWPIAFIPIVLCMACNSLFKIEMR"
BASE COUNT         2382 a          784 c         1063 g         2004 t
ORIGIN      
        1 atatgaaggc actattcaaa tgtaaaaact atcttcaaat tgataccaaa attgacatga
       61 ttaacaatta tttacatcaa gatatagcaa caaatatatc tgaagcagcg tacttattgt
      121 ggcttttatc aagaaataat attggattca cagatttgaa agttcttcac aaccgtttta
      181 ttgaaaagta tggatttgaa caactcgtga atattaaaga tttgatttca gatataacag
      241 gtttcgggac aacaattttt caagaagaag aaaccgatgg aaataatatt gtaatgctta
      301 agcaaaaatt tttacatgcc ttgagaaata acgatgagat agtcataaat gaaaaagatg
      361 tagagtcatt gattaacgat aatgaaatca atcattatca tgcgccaatg agtgcagatg
      421 tttatgctga actttattta ggacgttttt acaatcagta caatgagctc attgttatta
      481 gtcctttaac ggcgtctttt aatgctggcg ctacatttgg gcgatttcat catttaattg
      541 atactgaaac tttagcaaaa ttagaacatg aaaaaggaca ttattatcag aagatgatat
      601 gtgatgacaa tgtagaaatg atttctataa ataacatacc gaaatatccg agaaatcata
      661 atgtattaac taatcatgac tcatacgaat attcattgaa tttaggaagt agtaatagtt
      721 attcaaagta tgagcttacc ttagatgata tttatgttgg tgctaccttt aacaaattat
      781 atttatattc tagccaacta aataaaaggg tactatttga atcaaacaat atgtataact
      841 ttttaaaaga atgtaattta tatcgattgt tacgagaaat ttcaatggaa tctgtgaaat
      901 gcattgaacc tatgaatgat gtgagtattg attcatttag ttattcaccg aatcagatat
      961 aaaaatgtca ttcttaaacc agcatattgg aaaattaacg agatggtatt acctttacct
     1021 aaaaatgaag aatgggatca acaatttttg aaatatcaag aacaattcaa tatacctaat
     1081 atcgtgaatt tagtttatgg agataacaag cttctactaa atttatcatt agctaatcat
     1141 agatatttat tgatgaaaga atataagaag cataaacgtg tacgtttagt agaatcattt
     1201 ttacctcaat caaagaatga tcatgtgtat gaaattgtta caccaattta taaaaaatca
     1261 tcttatcgag gacccgaaat cgaaattcca aaatataaaa atacagatat tgaatatgac
     1321 aaagactggt ttgcattaca tattcatatc gataagccat cgcaagatac atttatcatc
     1381 gacaatttgt atccatttgt aaaacatctt aaagataagg gagatataga tcaatatttt
     1441 ttaatgcgtt atatcaaaca aggtgacatt ttaaagttga ggttattcag gaatgacgaa
     1501 aattacgcag aaatatacag catcttgaag aattggttac cacatgttcg acaaacgaca
     1561 gaagtttcag attatgaatt tgtatcatac gagccagaat tttttagata tggtggaaag
     1621 aacacgatta atgaaataga agccttcttt gaatatgaca cgaatttagc tgtcaatatt
     1681 attgaaaatg attttaagtt tgatcgacca tatattgttg ctatttcaat aatgtattta
     1741 tttgagatgt tttcaatttc taatgaagag cgtatggaaa ttgttaataa ctatgtacct
     1801 acatcattta aaagtaaaga tattagacct tttaaaaatg aacttgtaac tatatgtaat
     1861 cctgctaata attttgaata tatggctaaa cactatagtg gtatttatcg aattttaaaa
     1921 gatggcaacc aaattttgag caaattaaat gaagggctta aaaaaacttt aactactaaa
     1981 aggtcaagga ttattggcag cttaattcat atgcgctgca atcgaatatt tggcattgat
     2041 aaggatcaag aaacatttgt tttatctatt gtaaaagaaa ttgttaaaac tcaaaagcat
     2101 tggtgtggtg acaaaaatga ttgatatgaa tctaatattg aagaagaagt ttcaagagat
     2161 ttcagaagta gatgatttca tttcaaaagc atctactgaa accgattatt ttgaaccttc
     2221 aactttatca catggtatac caggtatcat tttgttttta gatgcttatc aaaaagtttt
     2281 cgatattaat actgagcaaa ttgtgcataa gtacatcatg aaattagcac catatttaca
     2341 gagtggtcaa tataacaatt ctttgtttgg tggactttca ggtattgcat tttcaatgga
     2401 tttagcatcg caaaatggca aaaattatca aaatatttta aataatattg atggtcacat
     2461 tgtaaatgaa atagaaaata aatttgatca aatattacaa gaaccattaa atccattaaa
     2521 ttatgatact gtcagtggat tagctgggat agggagatat ttgctaaata gagtagatga
     2581 gaatgaattt aatgttaaag cattaaaaag catattagta tactttaaag atattcaata
     2641 ttctaaaaat agctgggtag tcccacaaga aagtcaattt ttagagtctg ataaaaatta
     2701 ttttactgaa ggtaatatca atcttggcct tgcacatgga gtgctaggac cgatgtcttt
     2761 atttgcatta tgcgtgatta aaggaattac gattgaaaat catcagcaca tattaaaaga
     2821 catgtacaaa tttatcatgg acgaaaaatt ttgtaaccac gaaagatggt tgcagcgtta
     2881 cgatttaatt tctgaacgta atcatttcaa ttttattcgg aatggttggt gttatggcaa
     2941 tacgggtgta atgacgacgt tgtttttaat cggccaagca ttacaagatg atgaaataat
     3001 taaaatgtct aaaaaagtga tgctacaagt agtaaatgat aaagatgaaa atttaataag
     3061 tccaactatt tgtcatggat tgtcatcaca aatattaatg ttaacaatta tgaatttgaa
     3121 ttttgaatta aatgaagtgt ctgattatat cactgtatta ataaataaac tgatttctca
     3181 ttataaggaa gattatctgg tgaattttat agacattaat gaaaataagc aagatgtatt
     3241 taaaagtagg aaagttggcc ttttagaagg tgaattaggg gtctatctaa cattgatgac
     3301 actgaaaaat acaaaaattc tcaatgaaaa aaattggacc aatgcgtttt taattagctg
     3361 aatggaggcg tatatcatgg gagaaaatgt attgatttgt ttgtgtggct cagttaatag
     3421 tatcaatatt agccactata ttattgaatt aaaatcaaaa tttgatgaag taaatgtaat
     3481 cgcgtcaaca aacggtagaa agtttattaa tggtgaaata ttaaagcaat tttgcgataa
     3541 ctattatgac gaatttgaag accctttttt aaatcatgta gatatagcaa ataaacatga
     3601 caagattatt attttacctg cgacttctaa tacgattaat aaaattgcaa atggtatatg
     3661 tgacaattta ttattaacta tttgtcatac agcttttgaa aaactttcta tatttccaaa
     3721 tatgaattta cgaatgtggg aaaatccagt tactcaaaat aatattcgat tattaaaaga
     3781 ttatggtgta tcaatatatc cagcaaatat ttcagaaagt tatgaattag cgtcaaaaac
     3841 atttaaaaag aatgttgtcg cacctgaacc atataaagtt ctggaattca tttgagatta
     3901 gaatatgaaa atcataaagc gcgctattat tagtttaata atattgagtc ttttaatatc
     3961 tattacaatg agtaatgcat cagcgtcaga agaactatat tacagtgttg aatacaaaaa
     4021 tacagcaaca tttaacaagt tagttaaaaa gaagtcctta aacgttgtct ataatatacc
     4081 ggaattacat gtggcacaga ttaaaatgac gaaaatgcat gctaatgctt tagcaaacta
     4141 taaaaatgat attaaatata tcaatgccac atgttcaact tgtattacta gcgagaaaac
     4201 aatagacaga acatcaaatg agtcattatt ttcaagacaa tgggatatga ataaaataac
     4261 caataatggt gcatcgtatg atgatttgcc aaaacatgct aacaccaaaa tagcaatcat
     4321 agatacaggt gtgatgaaaa accatgacga tttgaaaaat aatttctcga ctgattctaa
     4381 aaatttagta cctttaaacg gttttagagg tactgaaccg gaggaaacag gtgatgttca
     4441 cgatgtcaat gataggaaag gacatggcac gatggtgtcg ggtcaaacga gtgctaatgg
     4501 taagttaata ggtgttgcac cgaataacaa atttacaatg tatcgcgtgt ttggtagtaa
     4561 aaaaacagaa ctgctttggg tatcaaaagc gattgttcaa gctgcaaatg atggaaatca
     4621 agtcattaat attagtgttg gtagttatat tattttggac aaaaatgacc atcaaacatt
     4681 tagaaaagat gaaaaagtag aatacgatgc gttacagaaa gcaatcaatt acgccaagaa
     4741 gaaaaaatct atcgttgttg ctgcagctgg taatgatggt attgatgtca atgacaaaca
     4801 gaaactaaaa ttacagcgtg aatatcaagg taatggcgaa gtgaaagatg ttcctgcatc
     4861 tatggacaat gtcgttacag taggatctac agatcaaaag agtaatctat ctgagttttc
     4921 caattttggt atgaattata cagatattgc tgcgcccgga ggatcatttg cttatttaaa
     4981 tcaattcggt gtggataaat ggatgaatga agggtatatg cataaggaga acattttaac
     5041 tactgccaat aacggaagat atatttatca agctggaact tcattagcca cacctaaagt
     5101 ttcgggagca ctagctttaa tcattgataa atatcatctt gaaaaacatc cagataaagc
     5161 gattgaattg ttatatcagc atgggacatc taagaataat aaaccattta gtagatatgg
     5221 gcatggtgag cttgatgtgt ataaagcatt aaatgtagca aatcaaaaag caagttaata
     5281 aatcaaagga gtttttgatt atggcaaaat tagttactga aaacatttcg aagcggttta
     5341 aaaatcaaga tgtattaaag catattaata tcactttaga aaataacgaa gtttatggat
     5401 tacttggtat taatggagcc ggtaaaacga cacttatgaa aattatatgt ggcatacttc
     5461 aacaagattc aggggaaatt aaattagata atagaccaat gacacgaaat gatttgcaca
     5521 aagttggttc gcttattgaa acacctgcga catataatca tttaagtgca caagataatt
     5581 tgaaaattgt gtgtttaaat gaaagcgttg atttcagcga aattaatagt gttttaagct
     5641 tagtcaattt aaatgtcgat aaaaagaaaa aggttaagga cttttcttta ggtatgaaac
     5701 aaagacttgg aattgcaatg gcattaatta aaaagccaga aattttagta ttagacgaac
     5761 catctaatgg tttagaccca tatggaatcc aagaacttag agaacttcta aaattattaa
     5821 cagaacaagg tactagtatt attatttcaa gtcacatttt atctgaaatc caagttttag
     5881 cagatcatat cggtattatt catgagggtg agctaaaata tcagcaaaga aataacaaag
     5941 atgaaaactt agaagagata ttcttcaaaa taacgaaagg tgattacaaa tgatacattt
     6001 aaagatagaa ggtatcaaat ttaaaaattc tttcagtatg tatgttttat taataagtcc
     6061 gctggtattt ctttgttttg ctattttcac agtcttattc gccaaaagta atacgggaac
     6121 agcgaatagt gtgtcaccat atataacttt actatttaat atttggccaa ttgctttcat
     6181 cccgattgta ttatgtatgg cttgtaattc gttatttaaa attgaaatga gaa
//
LOCUS       DALAGF010000134         6026 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.134, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000134 DALAGF010000000
VERSION     DALAGF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.134"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            277..1188
                     /gene="rapZ"
                     /locus_tag="O2692_002513"
     CDS             277..1188
                     /gene="rapZ"
                     /locus_tag="O2692_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCZ4300114.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1185..2180
                     /locus_tag="O2692_002514"
     CDS             1185..2180
                     /locus_tag="O2692_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCZ4300115.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2290..3234
                     /gene="whiA"
                     /locus_tag="O2692_002515"
     CDS             2290..3234
                     /gene="whiA"
                     /locus_tag="O2692_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCZ4300116.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            3808..4395
                     /gene="clpP"
                     /locus_tag="O2692_002516"
     CDS             3808..4395
                     /gene="clpP"
                     /locus_tag="O2692_002516"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCZ4300117.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(4638..5540)
                     /locus_tag="O2692_002517"
     CDS             complement(4638..5540)
                     /locus_tag="O2692_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCZ4300118.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKALFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
BASE COUNT         2193 a          827 c         1091 g         1915 t
ORIGIN      
        1 cctgtagaat ttcttttcga aattctctgt gttggggccc ctgactagag ttgaaaaaag
       61 cttgttgcaa gcgcattttc attcagtcaa ctactagcaa tataatatta tagaccctag
      121 gacattgatt tatgtctcaa gctcctttta aatgatgtat atttttagaa atttaatcta
      181 gacatagttg gaaataaata taaaacatcg ttgcttaatt ttgtcataga acatttaaat
      241 taacatcatg aaattcgttt tggcggtgaa aaaataatgg ataataatga aaaagaaaaa
      301 agtaaaagtg aactattagt tgtaacaggt ttatctggcg caggtaaatc tttggttatt
      361 caatgtttag aagacatggg atatttttgt gtagataatc taccaccagt gttattgcct
      421 aaatttgtag agttgatgga acaaggaaat ccatccttaa gaaaagtggc aattgcaatt
      481 gatttaagag gtaaggaact atttaattca ttagttgcag tagtggataa agtcaaaagt
      541 gaaagtgacg tcatcattga tgttatgttt ttagaagcaa gtactgaaaa attaatttca
      601 agatataagg aaacgcgtcg tgcacatcct ttgatggaac aaggtaaaag atcgttaatc
      661 aatgcaatta atgatgagcg agagcatttg tctcaaatta gaagtatagc taattttgtt
      721 atagatacta caaagttatc acctaaagaa ttaaaagaac gcattcgtcg atactatgaa
      781 gatgaagagt ttgaaacttt tacaattaat gtcacaagtt tcggttttaa acatgggatt
      841 cagatggatg cagatttagt atttgatgta cgatttttac caaatccata ttatgtagta
      901 gatttaagac ctttaacagg attagataaa gacgtttata attatgttat gaaatggaaa
      961 gagacggaga ttttctttga aaaattaact gatttgttag attttatgat acccgggtat
     1021 aaaaaagaag ggaaatctca attagtaatt gccatcggtt gtacgggtgg acaacatcga
     1081 tctgtagcat tagcagaacg actaggtaat tatctaaatg aagtatttga atataatgtt
     1141 tatgtgcatc atagggacgc acatattgaa agtggcgaga aaaaatgaga caaataaaag
     1201 ttgtacttat cggtggtggc actggcttat cagttatggc taggggatta agagaattcc
     1261 caattgatat tacggcgatt gtaacagttg ctgataatgg tgggagtaca gggaaaatca
     1321 gagatgaaat ggatatacca gcaccaggag acatcagaaa tgtgattgca gctttaagtg
     1381 attctgagtc agttttaagc caactttttc agtatcgctt tgaagaaaat caaattagcg
     1441 gtcactcatt aggtaattta ttaatcgcag gtatgactaa tattacgaat gatttcggac
     1501 atgccattaa agcattaagt aaaattttaa atattaaagg tagagtcatt ccatctacaa
     1561 atacaagtgt gcaattaaat gctgttatgg aagatggaga aattgttttt ggagaaacaa
     1621 atattcctaa aaaacataaa aaaattgatc gtgtgttttt agaacctaac gatgtgcaac
     1681 caatggaaga agcaatcgat gctttaaggg aagcagattt aatcgttctt ggaccagggt
     1741 cattatatac gagcgttatt tctaacttat gtgtgaatgg tatttcagat gcgttaattc
     1801 attctgatgc gcctaagcta tatgtttcta atgtgatgac gcaacctggg gaaacagatg
     1861 gttatagcgt gaaagatcat atcgatgcga ttcatagaca agctggacaa ccgtttattg
     1921 attatgtcat ttgtagtaca caaactttca atgctcaagt tttgaaaaaa tatgaagaaa
     1981 aacattctaa accagttgaa gttaataagg ctgaacttga aaaagaaagc ataaatgtaa
     2041 aaacatcttc aaatttagtt gaaatttctg aaaatcattt agtaagacat aatactaaag
     2101 tgttatcgac aatgatttat gacatagctt tagaattaat tagtactatt cctttcgtac
     2161 caagtgataa acgtaaataa tatagaacgt aatcatatta tgatatgata atagagctgt
     2221 gaaaaaaatg aaaatagaca gtggttctaa ggtgaatcat gttttaaata agaaaggaat
     2281 gactgtacga tgagctttgc atcagaaatg aaaaatgaat taactagaat agacgtcgat
     2341 gaaatgaatg caaaagcaga gctcagtgca ctgattcgaa tgaatggtgc acttagtctt
     2401 tcaaatcaac aatttgttat aaatgttcaa acggaaaatg caacaacggc aagacgtatt
     2461 tattcgttga ttaaacgtgt ctttaatgtg gaagttgaaa tattagtccg taaaaaaatg
     2521 aaacttaaaa aaaataatat ttatatttgt cgtacaaaga tgaaagcgaa agaaattctt
     2581 gatgaattag gaattttaaa agacggcatt tttacgcatg aaattgatca ttcaatgatt
     2641 caagatgacg aaatgagacg cagttacttg agaggagctt ttctggcagg tggctcagtg
     2701 aataaccctg aaacatcttc gtaccatttg gaaatttttt ctcaaaatga gagtcatgca
     2761 gaaggcttaa cgaaactaat gaatagttat gagttgaatg ccaaacattt agagcgaaaa
     2821 aaaggaagta ttacgtattt aaaagaagcg gaaaagattt cggattttct tagtttgata
     2881 ggtggctatc aagcgttatt aaaatttgaa gacgtacgta ttgtaagaga tatgcgtaat
     2941 tctgttaacc gactcgttaa ttgtgaaacg gccaatctaa ataaaacagt tagtgctgcg
     3001 atgaaacaag ttgagagcat taaattgatt gataaagaaa ttggtattga aaatttacca
     3061 gacaggttga gagagattgc tagaattcga gtagaacatc aagaaatttc gttgaaagag
     3121 cttggagaaa tggtatcaac tggtccaatt tcaaaatcag gtgtaaatca ccgattaaga
     3181 aaactgaatg atttagccga taagattaga aatggtgaac aaatagaatt ataagtaaga
     3241 aggtgttttt ggtagtgaat tatcaaaaac acttttttta tgattaaaaa gtgtttaatg
     3301 aataatagtc gactaactat taaattgagc tggaaagttt aatgaaggaa attcaataac
     3361 caagtgatgc ctaattttct cattaaatag catcatatgt atcaatttgg tcataagaaa
     3421 atatgaatgt gataattaat tgagtccctg aaagtccctg atcatagaca aaaacaaaat
     3481 cagcataaat aagaatcccg acattgcggg attctctgta ttgaaaagta ttgtatttta
     3541 ttaaaacagc ctccttgaag ggaattgaac ccctatctta agaaccggaa tcttacgtgt
     3601 tatccattac accacaagga gcaaatatgt aatcatatct ctgtactatg agaagtaaaa
     3661 taagtacaat ctaaaataga tattatcagt ttacaaagga aagagaaaag cgtcaaacaa
     3721 tgtaactatt taaagtcaaa gtgtttgacc aaatttgact taatatgtaa aataatgagt
     3781 aacagttatt acaaggagga aatatagatg aatttaattc ctacagttat tgaaacaaca
     3841 aaccgcggtg aacgtgcata tgatatatac tcacgtttat taaaagaccg tattattatg
     3901 ttaggttcac aaattgatga caacgtagca aattcaatcg tatcacagtt attattctta
     3961 caagcgcaag actcagagaa agatatttat ttatacatta attcaccagg tggaagtgta
     4021 acagctggtt ttgcgattta tgatacaatt caacacatta aacctgatgt tcaaacaatt
     4081 tgtatcggta tggctgcatc aatgggatca ttcttattag cagctggtgc aaaaggtaaa
     4141 cgtttcgcgt taccaaatgc agaagtaatg attcaccaac cattaggtgg tgctcaagga
     4201 caagcaactg aaatcgaaat tgctgcaaat cacattttaa aaacacgtga aaaattaaac
     4261 cgcattttat cagagcgtac tggtcaaagt attgaaaaaa tacaaaaaga cacagatcgt
     4321 gataacttct taactgcaga agaagctaaa gaatatggct taattgatga agtgatggta
     4381 cctgaaacaa aataattcaa agtaaagagt agactaagct gtctgctctt tttgtatgag
     4441 taaaccaagg tgtcaataat ttgtttacta tactttgagc ggaaatatga ttgaatgaag
     4501 ctagttgaac cgtaactata tgaaatgttc ccttcaaagt agacattgaa aggaacattt
     4561 caatcctttg tttgtaagtc gctctagaca ttacatttag tacatatgtt gtttctaatg
     4621 ctcattaatg gtattgatta ttctttaatt aaatcttcaa gtgccatttt taaattacta
     4681 tatttaaatt ggaatcccaa tgcttgaatt ttattaggta atactttttg agtatccaat
     4741 actactgttg acatttgacc aagtatgaga cgcattgcaa gacttggtgc ccaagtttca
     4801 tgaggcttat gcatagctct tgctaaagtg tagccaaata aattttgacg ttcaggtata
     4861 ggtgcagtta aattaaacgg accactagct gactcgttat ttattaaaaa taaaatagct
     4921 tgaattaaat cattgatatg aatccatgaa taccattgtt gaccagaacc taatttacca
     4981 ccaatgtaat attcgtatgg tagtttcatt gtttgtaacg caccgccttc attcgataaa
     5041 attataccga aacgaccgat gacaactcgt gtacctaatt gttcaaattg ttgtgcgaaa
     5101 cgttcccatt gatacacaat atctgataag aaatcaaaag gtaaagtttt ataaacttct
     5161 gtgtaactca taaataaatc aggaggatag taaccagtgg cactagcatt aaataaagct
     5221 ttaggtgctt tattacgtga tttaaacaat tcatataaag cttgcgtaga ttgaattcta
     5281 cttagcatta gcgtttgttt atattccggt gtccatcgtt tattcaatgt agcacctgct
     5341 aagttgatga ccacatcgat attttgagga actttgtgtt cccacccaga tttagcccag
     5401 ttgacatatg aaattttctt atcatttgaa atttggtcgt gtcgcgttaa tatcgtgata
     5461 tgtgaatctg attttttaat ttcattaact aattgagatc caaccatacc agtcccacca
     5521 gtaattaagt attgtttcat tatcattcac cccatgtaat tttgtattta gttactattt
     5581 aataatcatt attgttgttc aaaggttata cattatttag acaataatat gtcaataact
     5641 ttttttgaat tgtattttaa taaaaatgat ataagttata ataaaggtgt accttcgata
     5701 acgaataaac atctcttaaa agtatgtgta aaacgctgca tgatacaaac gaaggtaaaa
     5761 atttgactcc ctttagtagt ggacccgtac gttaatcgtg cgggtcgttt ttatttattt
     5821 cttattccca ttatacatca atttaaagca ttaatttttt aaacaaattt aagaatacat
     5881 agtaatataa caatctaaac ataaaaactt ttaacacaac acttaaacca atgctttaat
     5941 tttcaatacg tagctataat ttgttgtaaa atcaaaaagg ttaaaatgtt aattttcaaa
     6001 aaaggctcaa aatatgtttg atttag
//
LOCUS       DALAGF010000135         5801 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.136, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000135 DALAGF010000000
VERSION     DALAGF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.136"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            26..1006
                     /locus_tag="O2692_002518"
     CDS             26..1006
                     /locus_tag="O2692_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCZ4300119.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNNHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            969..1661
                     /locus_tag="O2692_002519"
     CDS             969..1661
                     /locus_tag="O2692_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCZ4300120.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDKLEEVRKSYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            1871..3037
                     /locus_tag="O2692_002520"
     CDS             1871..3037
                     /locus_tag="O2692_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCZ4300121.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHNDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRIVFTAHGWAFTEGVKPAKKFLYLVIEKLMSLITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENEVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            3018..4256
                     /locus_tag="O2692_002521"
     CDS             3018..4256
                     /locus_tag="O2692_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCZ4300122.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMSVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            4246..5676
                     /locus_tag="O2692_002522"
     CDS             4246..5676
                     /locus_tag="O2692_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCZ4300123.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVINLVLCIILIYFYGIYGAAIARLITEFF
                     LLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
BASE COUNT         1889 a          793 c         1054 g         2065 t
ORIGIN      
        1 gatatatttt taatgtataa atgaggtgga agatttggaa agagttttga taactggtgg
       61 ggctggtttt attgggtcgc atttagtaga tgatttacaa caagattatg atgtttatgt
      121 tctagataac tatagaacag gtaaacgaga aaatattaaa agtttggctg acgatcatgt
      181 gtttgaatta gatattcgtg aatatgatgc agttgaacaa atcatgaaga catatcaatt
      241 tgattatgtt attcatttag cagcattagt tagtgttgct gagtcggttg agaaacctat
      301 cttatctcaa gaaataaacg tcgtagcaac attaagattg ttagaaatca ttaaaaaata
      361 taataatcat ataaaacgtt ttatctttgc ttcgtcagca gctgtttatg gtgatcttcc
      421 tgatttgcct aaaagtgatc aatcattaat cttaccatta tcaccatatg caatagataa
      481 atattacggc gaacggacga cattaaatta ttgttcgtta tataacatac caacagcggt
      541 tgttaaattt tttaatgtat ttgggccaag acaggatcct aagtcacaat attcaggtgt
      601 gatttcaaag atgttcgatt catttgagca taacaagcca tttacatttt ttggtgacgg
      661 actgcaaact agagattttg tatatgtata tgatgttgtt caatctgtac gcttaattat
      721 ggaacacaaa gatgcaattg gacacggtta taacattggt acaggcactt ttactaattt
      781 attagaggtt tatcgtatta ttggtgaatt atatggaaaa tcagtcgagc atgaatttaa
      841 agaagcacga aaaggagata ttaagcattc ttatgcagat atttctaact taaaggcatt
      901 aggatttgtt cctaaatata cagtagaaac aggtttaaag gattacttta attttgaggt
      961 agataatatt gaagaagtta cagctaaaga agtggaaatg tcgtgaaaat gacattgaag
     1021 ctgtccataa taataagggt tatgcctatc aaagaaaatt agacaaacta gaagaagtga
     1081 gaaaaagcta ttacccaatt aaacgtgcga ttgacttaat tttaagcatt gttttattat
     1141 ttttaacttt accgattatg gttatattcg ccattgctat cgtcatagat tcgccaggaa
     1201 accctattta tagtcaggtt agagttggga agatgggtaa attaattaaa atatacaaat
     1261 tacgttcgat gtgcaaaaac gcagagaaaa acggtgcgca atgggctgat aaagatgatg
     1321 atcgtataac aaatgtcggg aagtttattc gtaaaacacg cattgatgaa ttaccacaac
     1381 taattaatgt tgttaaaggg gaaatgagtt ttattggacc acgcccggaa cgtccggaat
     1441 ttgtagaatt atttagttca gaagtgatag gtttcgagca aagatgtctt gttacaccag
     1501 ggttaacagg acttgcgcaa attcaaggtg gatatgactt aacaccgcaa caaaaactga
     1561 aatatgacat gaaatatata cataaaggta gtttaatgat ggaactatat atatcaatta
     1621 gaacattgat ggttgttatt acaggggaag gctcaaggta gtcttaattt acttaataag
     1681 ttcaaataaa agttatattt taaagattgt gaccaattgt tacagtataa cgaggaatcc
     1741 cttgagacag tatcaaatgg cattaagaaa tatgtgccat cattgatttg catggctata
     1801 aatactattc atctgatgag atagccatgt taagaaattg aaagtatagc attaaagggg
     1861 tttgtaacag ttgaaaatta tatattgtat tactaaagca gacaatggtg gtgcacaaac
     1921 acatctcatt caactcgcca accatttttg cgtacacaat gatgtttatg tcattgtagg
     1981 caatcatgga ccaatgattg aacaactaga tgcaagagtt aatgtaatta ttatcgaaca
     2041 tttagtaggt ccaattgact ttaaacaaga tattttagct gtcaaagtgt tagcacagtt
     2101 attctcgaaa attaaacctg atgttatcca tttacattct tccaaagctg gaacggtcgg
     2161 acgaattgcg aagttcattt cgaaatcgaa agacacacgt atagttttta ctgcacatgg
     2221 atgggctttt acagagggtg ttaaaccagc taaaaaattt ctatatttag ttatcgaaaa
     2281 attaatgtca cttattacag atagcattat ttgtgtttca gatttcgata aacagttagc
     2341 gttaaaatat cgatttaatc gattgaaatt aaccacaata cataatggta ttgcagatgt
     2401 tcccgctgtt aagcaaacgc taaaaagcca atcacataac aatattggcg aagtagttgg
     2461 aatgttgcct aataaacaag atttacagat taatgccccg acaaagcatc aatttgttat
     2521 gattgcaaga tttgcttatc caaaattgcc acaaaatcta atcgcggcaa tagagatatt
     2581 gaaattacat aacagtaatc atgcgcattt tacatttata ggcgatggac ctacattaaa
     2641 tgattgtcag caacaagttg tacaagctgg gttagaaaat gaagtcacat ttttgggcaa
     2701 tgtcattaat gcgagtcatt tattatcaca atacgatacg tttattttaa taagtaagca
     2761 tgaaggtttg ccaattagca ttatagaagc tatggctaca ggtttgcctg ttatagccag
     2821 tcatgttggc ggtatttcag aattagtagc tgataatggt atatgtatga tgaacaacca
     2881 acccgaaact attgctaaag tcctggaaaa atatttaata gacagtgatt acatcaaaat
     2941 gagtaatcaa tctagaaaac gttatttaga atgttttact gaggagaaaa tgattaaaga
     3001 agtggaagac gtttataatg gaaaatcaac acaatagtaa attactaaca ttgttactta
     3061 tcggtttagc ggtttttatt cagcaatctt cggttattgc cggtgtgaat gtttctatag
     3121 ctgactttat cacattacta atattagttt atttactgtt tttcgctaac catttattaa
     3181 aggcaaatca ttttttacag tttttcatta ttttgtatac atatcgtatg attattacgc
     3241 tttgtttgct attttttgat gatttgatat ttattacggt taaggaagtt cttgcatcta
     3301 cagttaaata tgcatttgta gtcatttatt tctatttagg gatgatcatc tttaagttag
     3361 gtaatagcaa aaaagtgatc gttacctctt atattataag cagtgtgact ataggtctat
     3421 tttgtattat agctggtttg aacaagtccc ctttactaat gaaattgtta tattttgatg
     3481 aaatacgttc aaaaggatta atgaatgacc ctaactattt cgcgatgaca cagattatta
     3541 cattggtact tgcttacaag tatattcata attacatatt caaggtcctt gcatgtggta
     3601 ttttgctatg gtctttaact acaacggggt ctaagactgc gtttatcata ttaatcgtct
     3661 tagccattta tttctttatt aaaaagttat ttagtagaaa tgcggtaagt gttgtgagta
     3721 tgtcagtgat tatgctgata ttactttgtt ttacctttta taatatcaac tactatttat
     3781 tccaattaag cgaccttgat gccttaccgt cattagatcg aatggcgtct atttttgaag
     3841 agggctttgc atcattaaat gatagtgggt ctgagcgaag tgttgtatgg ataaatgcca
     3901 tttcagtaat taaatataca ctaggttttg gtgtcggatt agtggattat gtacatattg
     3961 gctcgcaaat taatggtatt ttacttgttg cccataatac atatttgcag atctttgcgg
     4021 aatggggcat tttattcggt gcattattta tcatatttat gctttattta ctgtttgaat
     4081 tatttagatt taacatttct gggaaaaatg taacagcaat tgttgtaatg ttgacgatgc
     4141 tgatttactt tttaacagta tcatttaata actcaagata tgtcgctttt attttaggaa
     4201 ttatcgtctt tattgttcaa tatgaaaaga tggaaaggga tcgtaatgaa gagtgattca
     4261 ctaaaagaaa atattattta tcaagggcta taccaattga ttagaacgat gacaccactg
     4321 attacaatac ccattatttc acgtgcattt ggtcccagtg gtgtgggtat tgtttcattt
     4381 tctttcaata tcgtgcaata ctttttgatg attgcaagtg ttggcgttca gttatatttt
     4441 aatagagtta tcgcgaagtc cgttaacgac aaacggcaat tgtcacagca gttttgggat
     4501 atctttgtca gtaaattatt tttagcgtta acagtttttg cgatgtatat ggtcgtaatt
     4561 actatattta ttgatgatta ctatcttatt ttcctactac aaggaatcta tattataggt
     4621 gcagcactcg atatttcatg gttttatgct ggaactgaaa agtttaaaat tcctagcctc
     4681 agtaatattg ttgcgtctgg tattgtatta agtgtagttg ttatttttgt caaagatcaa
     4741 tcagatttat cattgtatgt atttactatt gctattgtga cggtattaaa ccaattacct
     4801 ttgtttatct atttaaaacg atacattagc tttgtttcgg ttaattggat acacgtctgg
     4861 caattgtttc gttcgtcatt agcatactta ttaccaaatg gacagctcaa cttatatact
     4921 agtatttctt gcgttgttct tggtttagta ggtacatacc aacaagttgg tatcttttct
     4981 aacgcattta atattttaac ggtcgcaatc ataatgatta atacatttga tcttgtaatg
     5041 attccgcgta ttaccaaaat gtctatccag caatcacata gtttaactaa aacgttagct
     5101 aataatatga atattcaatt gatattaaca atacctatgg tctttggttt aattgcaatt
     5161 atgccatcat tttatttatg gttctttggt gaggaattcg catcaactgt cccattgatg
     5221 accattttag cgatacttgt attaatcatt cctttaaata tgttgataag caggcaatat
     5281 ttattaatag tgaataaaat aagattatat aatgcgtcaa ttactattgg tgcagtgata
     5341 aacctagtat tatgtattat tttgatatat ttttatggaa tttacggtgc tgctattgcg
     5401 cgtttaatta cagagttttt cttgctcatt tggcgattta ttgatattac taaaatcaat
     5461 gtgaagttga atattgtaag tacgattcaa tgtgtcattg ctgctgttat gatgtttatt
     5521 gtgcttggtg tggtcaatca ttatttgccc cctacaatgt acgctacgct gctattaatt
     5581 gcgattggta tagtagttta tcttttatta atgatgacta tgaaaaatca atacgtatgg
     5641 caaatattga ggcatcttcg acataaaaca atttaagtac cggtaatgct atactttaga
     5701 aaattaagat taagaagaaa aggcaatttc ttattgaaaa atggaagttg tcttttttaa
     5761 ttctctttaa aagcgggaaa caaaagcagt taaatgcctt t
//
LOCUS       DALAGF010000136         5636 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.137, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000136 DALAGF010000000
VERSION     DALAGF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.137"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..289)
                     /locus_tag="O2692_002523"
     CDS             complement(<1..289)
                     /locus_tag="O2692_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HCZ4300124.1"
                     /translation="MSVNARDLLVLHTNVNRLVGEEIFANKCLANNDVQIMNSIKKLI
                     EAELLTTTNDFEVSIYKKTRPELQSILKSFGIKTTGNKPDLIKRIDDNFHII"
     gene            578..670
                     /locus_tag="O2692_002524"
     CDS             578..670
                     /locus_tag="O2692_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300125.1"
                     /translation="MFIFKTTSKSHFHNNVKSLECIKIPINKNR"
     gene            complement(1375..2232)
                     /locus_tag="O2692_002525"
     CDS             complement(1375..2232)
                     /locus_tag="O2692_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCZ4300126.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAEHFLAE"
     gene            complement(2300..3082)
                     /locus_tag="O2692_002526"
     CDS             complement(2300..3082)
                     /locus_tag="O2692_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4300127.1"
                     /translation="MLIQLDQIGRMKQGKTILKKISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATSGTVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEIRNEAHQLLKLVGMFAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPTLAMIYVTHFIEEITANFSKILLLK
                     DGQSIQQGAVEDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            complement(3314..5119)
                     /gene="glmS"
                     /locus_tag="O2692_002527"
     CDS             complement(3314..5119)
                     /gene="glmS"
                     /locus_tag="O2692_002527"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCZ4300128.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
BASE COUNT         1821 a         1048 c          735 g         2032 t
ORIGIN      
        1 taataatatg aaaattgtcg tcaatacgtt taattaagtc aggtttatta cctgttgttt
       61 ttataccaaa actttttaaa atactttgta attcaggtct tgtcttttta tatatactaa
      121 cttcaaaatc attagtcgtt gttaataact cagcttcaat taattttttt attgaattca
      181 taatttgaac atcattatta gcaaggcact tattagcaaa tatctcttct ccaaccaatc
      241 tatttacatt agtgtgtaat actaataaat cacgtgcatt tacgctcact agctttcatc
      301 tcctcatttt tgcttttcaa atttattaag tatgaataaa aaatacatcc gaacaaacaa
      361 aatctctata ttctaattcg atttacatag attatacact aaaaaaatga ttctcaatgc
      421 aaatgaatgt gtctctcata gtctaaaaac tcgtatgttt cgaattttat gattcattga
      481 taatagtacc agttaaaaga actaagattg taattatccc cccttaaaat tcaagaatat
      541 attttcttaa tctatcagca agaggttata tttcaaagtg tttattttta aaacaacatc
      601 taaaagccat tttcataata atgttaaatc attagaatgt ataaagattc caattaataa
      661 aaatagataa aatgcaataa agttccaaat acttattatt attcatcaca aattacgtga
      721 tgccctctac aacactaaat caaggatgat atttatttta tatacattag cattcattgt
      781 ttgcttaccc acattatcct gtttagtctt ttttataact ctagtcttga atgtcatatt
      841 tacaaagaaa aaaacactaa aatatttaaa actaacacta tttattcact ttgttctttt
      901 atagcacctt ttatgtttat tttcttgtat tcaataaata gtatggagat gataatttaa
      961 agtagatata tttgaagtat actatagagt aaattatatt ggggaaatat gtaaacgaac
     1021 cggttgattt tatagtggat taacacttca tagagaatat aatcaagaac aaaaacagtc
     1081 aataggtgtg aattttttta aagacggatc tgttgctact aaaaaaagaa gtatacaatt
     1141 caatagttaa accttaaaac aagaaatatt attcaaaatc aatgaatttc ctatcttatt
     1201 agttttaaca atatttattc tcatagagtt tgtccaatta aatatagatg attcaaatat
     1261 ttataagcat acaaaagagc agtaagacat tttctaatag aaaataactt tactgctctt
     1321 tgtacattac tcatcaacta ttgtaaatta aataataaat aactacctat tttattattc
     1381 agctaaaaaa tgctctgcta aatattttgc tgcgccatcc tcttcattcg tgtatgccgt
     1441 tacatctgaa gttaatgctt ggatttcagg gcgtgcattt ttcatagcaa ctgtataatg
     1501 accaaattca aacattgctc tatcattgtc gctatctcca ataactaacg tttcttcttg
     1561 atgaatacca aaatgtgcaa tcatttcttt aatgcctgta cctttatcag tttgataagc
     1621 cattgtttcc gcattaaatc ttgatgaatt tgaaacacta atctgtagtt gcacatgatt
     1681 ttgctttaat tcatctctaa atgctgttat tttttctaaa ttagaactga atagataaat
     1741 ttttgaatat tcaccttcag gaaacttagt tacccaatct atcttaccag caagcgcatc
     1801 ttgtcttgaa gaccattcac tatgacttac gccattaata ggatcttgac tacgaatcat
     1861 atctcgcatc catgtttcat cttcttttaa agaaactcta ttaccttcaa aaggaaatac
     1921 ctcataataa atttgttggc gcttagctaa attagtaatt tgttgcactt gagccaatga
     1981 taaaccatgc ttgaagataa tttctccatc tacttctcca attgttccat ttgaactaat
     2041 gatgccatta accgcaaaat cttgaggtac aagttgatgt atttcagaat gcgaacgtcc
     2101 tgttgccaaa aatactttat atcctttctc tctcaattga ttaatgacat ctttcgtata
     2161 cgttgatgct tgattatttt catgtaaaat tgttccatcc atgtccaaaa atattgcttt
     2221 tacgttatcc atattgccct ccactgtcca ttttatctta attaaatttt gtcattatta
     2281 cttattattt gcaaaatatt tactctaaca ttgccataga aaatcgatta ttccatcttt
     2341 gaactgctac atttttctgg aaaaatcgtg acatgttttc agaagttaat atgtcttcta
     2401 cagcgccttg ttgaatactt tggccatctt ttagcagtaa aattttggaa aagttagcag
     2461 ttatttcttc aataaagtgc gtcacataaa tcatcgcaag cgttggatat gaatctgaca
     2521 atgagtcaag tatacttaac aacgattctc gtgcaataaa gtctaaacca gctgctggct
     2581 catctaaaat taaaacctgg ggttgcccca ttaaagctcg tgcaatcatc actcgttgtt
     2641 tttcaccggt agataaataa ccaatatatt gttgcgcttt agcaaacatt ccaactaatt
     2701 taagtaattg atgtgcttca ttacgtatct catcatcaat atcttgataa acaccaattg
     2761 atttaaaggc accgcttatc accacatcga ttactctttc accctcttga aacttttcca
     2821 gtaaactatg agatacaaaa cctatatgtt gtcgtacagt ctctgcagaa taccctacct
     2881 tgcctggcat tttaccgaaa aggttaacag ttccagatgt tgcaggctca tacgcattta
     2941 aaatatttag aagtgttgtc ttgccagcac cattcaaccc atataatatc catttatcac
     3001 ctttagcaat ttgccaagaa atctttttta aaattgtttt tccttgcttc attcgcccaa
     3061 tttgatctaa ttgaattaac atgcgaggtc acccctttat tgctactcca tacttctcat
     3121 aaaatcatat taacataaca cccttaattg tcagactatt taaataaata aaacacttca
     3181 tttttacgca tttctgccaa attaagatga agtaaaagct aagtcgacct aaaaaagcac
     3241 ccttctagtc gattaatcta aaagggtgcc atatacttta attttaatac atgattgatt
     3301 ctaaaaaagt gaattattcc acagtaactg atttagcaag gttacgtggt ttatcaacat
     3361 ctaaatctct gtgtaatgct gcatagtatg aaattaattg taatgcaacc actgatacta
     3421 atggcgttaa caattcatgt acatgaggaa tgacataagt gtcgccttct ttttcaagac
     3481 cctccataga aataatacat ggatgtgcac cacgtgctac tacctcttta acgttaccac
     3541 gaattgataa attaactttc tcttgtgttg ctaaacctac aactggtgta ccttcttcga
     3601 ttaaggcaat tgtaccatgt ttaagttctc caccagcaaa accttctgct tgaatgtaag
     3661 aaatttcttt aagttttaac gcaccttcta aacttacgtt atagtcaata gtacgtccga
     3721 taaagaatgc attgcgtgtt gtttctaaga aatctgtagc aatttgttcc ataattggtg
     3781 catcgtcaac aattgcttct attgctgttg ttacttttgc taattctctc aataaatcaa
     3841 tatctgcttc acgaccatgc tcttttgcaa cgatttgaga caagattgat aatactgcaa
     3901 tttgtgcagt atatgctttt gtagatgcaa ctgcgatttc aggacccgcg tgtaataaca
     3961 atgtgtggtc tgcttcacgt gataaagttg aacctgcaac attagtgatt gttaatgatt
     4021 tatgacctaa tttattagtt tcaactaata cggcgcggct atctgcagtt tcacctgatt
     4081 gagaaatata aacgaacaat ggtttttcag ataataatgg catgttgtag acaaactctg
     4141 atgcaacgtg tacttcagtt ggtacgccag cccatttttc taaaaattct ttacctacta
     4201 aacctgcatg gtagcttgta cctgctgcaa taacgtaaat gcggtctgct tctttaacat
     4261 cattgatgat gtcttgatca attttcaagt taccttctgc atcttgatat tcttgaataa
     4321 tacgacgcat tactgctggt tgttcatgaa tttcttttaa catgtagtgt gcataaacac
     4381 ctttttcagc atctgatgca tcaatttcag caatatatga atcacgttct acaacgtttc
     4441 catctgcatc tttaataata acttcatctt ttttaacaat aacgatttca tggtcatgga
     4501 tttctttata ttcgcttgtc acttgtaaca ttgcaagtgc gtctgatgcg ataacattga
     4561 aaccttcacc aacacctaat aataatggtg atttattttt agcaacatag attgtgtctt
     4621 tgtcttcagc atctaataaa cctaatgcat atgaaccatg taataatgac acaacttttg
     4681 taaatgcttc ttcagttgaa agtccttgat ttgaaaagta ttcaactaat tgaacgataa
     4741 cttctgtatc tgtttctgaa atgaatgata caccttgtaa gtattcacct tttaactctt
     4801 catagttttc aataacaccg ttatgaacta gagtaaaacg gccatttgat gattgatgtg
     4861 gatgagagtt ttcatgattc ggtacaccgt gtgttgccca acgtgtgtga ccgattccaa
     4921 caggtccatc aaaatcgcta ctatcagcaa ctttacgtaa ttctgcaata cgaccttttt
     4981 ctttaaatac agttgtatta tcatcattta ctactgcgat acctgcagag tcataacctc
     5041 tgtattctaa tttttctaaa ccttttaata ataattcttt ggcattatca tagccaatat
     5101 aaccaacaat tccacacata acgacatttt cctccatatt ggaatagtac gcgtaaatta
     5161 tgatttattg ccgataattt agattgacaa tctgctttca taatataaat aggaacatgc
     5221 tatcatcgca ttcatccata acaaattaag catagttatt tttacaacta tacaaattgc
     5281 tcacactgta ctttccatat taatattttt tatattcaat ttctggcgat cttattaact
     5341 ttgtccatta agtcacccta atgttttact taataagcta acgaatgagc cacatccggg
     5401 atagcatccg ccgatctatt cgatcactat cctcctcgtc tacaaataca tatattgcac
     5461 tctataaagg ccactcatat attaaccttt aatcttcaaa tacaaatatt tatttgcaca
     5521 ggcgctttaa ctgtactgcc gaactttccc cctttccatt aatcattatt gtacaacggt
     5581 gttgttttgt tttgcaaata ttttcacaat aaaattttaa aaatcctaaa acaatt
//
LOCUS       DALAGF010000137         5562 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.138, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000137 DALAGF010000000
VERSION     DALAGF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.138"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(107..1699)
                     /gene="pckA"
                     /locus_tag="O2692_002528"
     CDS             complement(107..1699)
                     /gene="pckA"
                     /locus_tag="O2692_002528"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCZ4300129.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     regulatory      1854..1964
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2070..3263
                     /locus_tag="O2692_002529"
     CDS             2070..3263
                     /locus_tag="O2692_002529"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCZ4300130.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     gene            3388..4296
                     /locus_tag="O2692_002530"
     CDS             3388..4296
                     /locus_tag="O2692_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HCZ4300131.1"
                     /translation="MDLSSPIVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNLKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            4508..5341
                     /locus_tag="O2692_002531"
     CDS             4508..5341
                     /locus_tag="O2692_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCZ4300132.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNNVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQRIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHAVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKKFE
                     G"
     gene            complement(5404..>5562)
                     /locus_tag="O2692_002532"
     CDS             complement(5404..>5562)
                     /locus_tag="O2692_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCZ4300133.1"
                     /translation="RGINKVKRELGFVLMALNIRKIAAQRAVHYKIHIKKADFYQIIN
                     RNQLFYIA"
BASE COUNT         1873 a          860 c         1033 g         1796 t
ORIGIN      
        1 gttggctgac ttgtcagtgc ccttttaggg ctggccagta aggaaggctt atagccgcag
       61 aacaaaccac ccgttcacac gggtggtttt tatttagtat tcaaatttat ttgttgaagc
      121 tacctttttc agcaatatgt tcaacttttt caccaaattt ttcgaagttc ttttcaaaac
      181 gttgaattaa atcttctgct tgtgctttat atttctcttt gtcgctccaa gcattaattg
      241 gatttaaaat tgttttcggt acatcttcaa tttctacagg aatgcttaaa ccaaacgtac
      301 tatcttttgt atattctgca tttttcaatt tgccagaaat cgcttggttt accatttgac
      361 gtgtgtaatg taagctgatt ctacgtccta caccatattt tccgccagtc catccagtat
      421 taacaagata aacatcaaca tcatgtaaat cgataagttc acctaataga tcagcgtaaa
      481 cagtagggtg taacgggaag aacggtgctc cgaaacatgt tgagaatgat ggttcaggtt
      541 ctgtcacacc acgctctgta ccagctaatt tagaagtgaa accactcaag aaatgataca
      601 ttgcttggtc tttatttaac tttgaaatcg gtggaataac accaaatgca tccgcagtta
      661 agaaaataat tgtatttgga tgtgctgctt tagatggtac tacaatattg tcaatgtgat
      721 taattggata agcggcacgc gtgttttctg tataacgatt gtcttcaaag tccactgaac
      781 catcttctgc aactacagtg ttctctaaaa ttgcaccata tttgattgcg tcaaaaatct
      841 gtggttcttt ttctttggaa agattaattg cttttgcata gcagccacct tcgatattaa
      901 agaccccgtt tttattccag ccgtgttcat catcaccgat tagtttacgg tgtgggtcag
      961 ccgataaggt tgttttacca gtgccagata gaccaaagaa taatgcaaca tcgccttttt
     1021 caccaacgtt tgctgagcaa tgcatgctca taatatcttg catcggtaag agataattca
     1081 ttacagagaa gatacctttt ttcatttcac cagcgtattc agtaccgccg attaaaatga
     1141 ctttgtgttt aaatgaaata atgacaaagg tttcagattt agtaccatca acttctggat
     1201 ctgctttaaa atgtggtgca gaaacgatag tgaagttagg tttaatcttt gtagcttctt
     1261 cttttgattc aggtctaata aacatatttt tagcaaataa attatgccat gctaattcat
     1321 tgatgactgt aagttttaac attgtatctt tatcgctacc agcgtagcct ttaaatacgt
     1381 atagttcatc ttttttatct aaatagtcta gtactttatg gtataacttc aagaaagttt
     1441 cttcatcgat aggttgatta atttctcccc aatcaatgtt atctctatat gaaggttcag
     1501 agacaaaaaa tttgtcttta ggcgaacgac cagtatattt tccagtactt gcattaacag
     1561 caccaagttc tgttaatacc ccttcgttat tgtctaagat tttattataa agttgtgtcg
     1621 tcgaaagttg aaaatgtgac gttggttttt tcagtaattt gtcaatttta gttgtttcag
     1681 tgtatgtgtc tactgacatc ctaaatccct ccaaagcgat aatttaaatg taagccttta
     1741 catttaatta gtataacaca ttaacataat taatctacaa tattttaccg taaatttatt
     1801 tgtgaaaaat tgacattaat tcattaatta ggtactatta tttttaacgg attctcttat
     1861 cctgagtggt ggagggacat ggacccaatg aaacccagca acctcttttt tataaaagaa
     1921 aggtgccaaa ccgtttgcag acaaataggt ctgaacgata agagcgaatg gacgtattag
     1981 gccttctctc tatattaata gttagaaggt cttttttatt tagctcacag agagagaatt
     2041 ttcgtaatat aaatttaaag gagcaaacta tgttaaataa caaacgatta tttacttcag
     2101 agtctgttac agaaggacac ccagataaaa tcgctgacca agtgtcagat gcaatattag
     2161 atgctatttt aaaagacgat ccaaatgcac gtgtagcttg tgaaacaact gttacaacag
     2221 gtatggcatt gatcgccggc gaaatttcta caacaacata tgttgatatt ccgaaagttg
     2281 ttagagaaac aattaaagaa attggataca caagagcaaa atatggttat gattatgaaa
     2341 caatggcaat tttaactgcg attgatgaac aatcacctga cattgcacaa ggcgtggata
     2401 aagcattaga gtatcgtgat aaagatagcg aagaagaaat tgaagcaaca ggtgcaggtg
     2461 accaaggttt aatgtttggt tacgcaacga atgaaacaga gacgtatatg cctctagcaa
     2521 tttatttatc acatcaattg gcaaaacgtt tatcagatgt gcgtaaagat ggtacgttaa
     2581 attatttacg accagacggt aaagttcagg tcactgttga atatgatgaa aatgataacc
     2641 cagtacgtat tgatacgatt gtcgtatcaa cacaacatgc tgaggatgtg acacttgaac
     2701 aaattcaaga agacattaaa gcacatgtca tttatccaac tgttccagaa aacttgatta
     2761 atgaacaaac taaattctac atcaatccta caggacgctt tgtaatcggt ggacctcaag
     2821 gtgatgcagg attaacagga cgtaaaatca ttgttgatac gtacggtggc tatgcacgtc
     2881 atggtggcgg atgcttcagt ggtaaggatc ctacaaaagt agaccgttca gctgcatacg
     2941 ctgcacgtta tgtggctaaa aatattgttg cagcaggttt agcagatcaa tgtgaagtac
     3001 aattggcata tgcgattggt gttgcagaac cagtgtcaat tgcaattgat acatttggaa
     3061 caggtaaagt ttctgaaggg caacttgtcg aagcagttag aaaacacttt gacctaagac
     3121 ctgcaggtat tattaaaatg cttgatttga aacaaccgat ttacaaacaa actgctgctt
     3181 atggtcattt tggacgtaca gatgtcttat tcccatggga aaaattagac aaagtggaag
     3241 aattaaaaga cgcagtaaaa taataagatg aattgaatat agctaaataa aagattttcg
     3301 gtatataaat gataatatgt atcgaaaatc tttagctttt ctattataat taaagttatt
     3361 catatattaa aaaggagcgt tgttgtgatg gatttatctt caccgatagt cattggactc
     3421 attattgcag tagtcattgc attaatcttt tttgtccttt ttttagttgc cttaggtagt
     3481 aagaaaaaag ttaagcgaca aacagaagaa aagtatgaac aacaagaaca aaacattaaa
     3541 aagtctcatg aagaagcttt agagaaagag cgtattcaaa ataaaaagac aattacaaaa
     3601 caacaagaag attataatca catggttagt acaaaagatc gtgagattga cgcattaaaa
     3661 ttgttctcga aaaatcatag tgaatatgta acggacatga gattaattgg tattcgtgaa
     3721 agattagtta aagaaaaacg aattcgacca gaagatatgc acattatggc aaatatcttt
     3781 ttaccaaagg atggctttaa taatattgaa cgaattagtc atttagtatt aacaagaact
     3841 ggtctttata ttattgattc tcaattgtta aaaggtcatg tttataatgg tattagtggc
     3901 ggtcaattta aagatttacc accaatggaa caagtatttg atacattgga tttagataaa
     3961 tctagaccgc aaactattgt gatggatcaa aatgatgaca aacgttcatt atcatttgta
     4021 aattattcag atcaaattga ggcgattaaa caattggctg aagacttaca aaaagaatta
     4081 ggtgccaaat atacaccaac ttcaatttta tactttaatc ttaaaaatga aggcgatgta
     4141 acgatttcaa attataatca aaatagtgct gtcaaagtgc ttgtcggtgc tgaacaatta
     4201 gacgagttct ttaacaaatt tgtattccat ggtcgtattc aatacaacgt ggaagactta
     4261 caacaaatga tggataaaat tgaatcattc aattaaatgt taggctgaag tgatgactat
     4321 atgttgttac ttcggctttt ttagtttgtc ataaattaaa cagaattact tttacaattt
     4381 gattaatatt tgagagatat tactagaatt ttagtggttg attgctctaa gtgttttttc
     4441 tcatgtattc aactcagatt tgttatgata acagggtata atacatcaaa aaggtgaggc
     4501 gatatttatg gaggttaaaa cattttataa tggaaacacg atgccacaaa ttggtttagg
     4561 gacatttcgt gtggaaaatg atgaaaattg catggaaagt gttaagtatg ccattgaaca
     4621 aggatatcgt agtattgata ccgcaaaagt ttatgggaat gaagaacaag taggtgctgg
     4681 cattcgtgcc ggattggagt caactggtat tgctagagaa gacttattta ttacttcaaa
     4741 attatatttt gaggatttcg gtcgtgaaaa tgtagcagct gcttacgaag ctagtttatc
     4801 tagattaggt ttaaaatact tagatttgta tctagtacat tggccaggta cgaacgaagc
     4861 cgtaatggtt gatacatgga aaggtatgga agatttatat aaaaataata atgttaaaaa
     4921 tataggtgtc agcaattttg aacctgaaca tttggaagct ttgttggcac aagtgtcaat
     4981 taaacctgta attaatcaag ttgaatatca tccatattta acccaacata aattgaaatt
     5041 atatttggca gcacaacgta tcgtgatgga atcttggtca ccattgatga atgcacaaat
     5101 tttaaatgat gagacaatta aagacattgc tcaagaatta ggaaagtcac ctgcccaagt
     5161 tgttttaaga tggaatgtgc agcatgctgt ggttacaatc cctaaatcgg tgacaccaaa
     5221 cagaatctct gaaaatttcc aaatatttga tttcgaatta tcagatgaac aaatgacgcg
     5281 aattgatggt ttaaatcaag ataagagaat tggacctgat ccaaaaaaat ttgaaggcta
     5341 gattaaaatc gctcaactga tgaaaaggtt agatgaattg tcagggcttg ggacattaag
     5401 ttcttaggca atgtaaaaaa gctgatttct attaattatt tgatagaaat cagctttttt
     5461 gatatgtatt ttataatgta cagctcgttg agctgctatt ttccttatat taagtgccat
     5521 taatacaaaa cctagctctc gtttaacttt atttattcct cg
//
LOCUS       DALAGF010000138         5432 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.139, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000138 DALAGF010000000
VERSION     DALAGF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.139"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            99..425
                     /locus_tag="O2692_002533"
     CDS             99..425
                     /locus_tag="O2692_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HCZ4300134.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            427..906
                     /gene="rsbW"
                     /locus_tag="O2692_002534"
     CDS             427..906
                     /gene="rsbW"
                     /locus_tag="O2692_002534"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HCZ4300135.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            881..1651
                     /gene="sigB"
                     /locus_tag="O2692_002535"
     CDS             881..1651
                     /gene="sigB"
                     /locus_tag="O2692_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HCZ4300136.1"
                     /translation="MAKESKSANEISPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHQ"
     gene            2085..4235
                     /locus_tag="O2692_002536"
     CDS             2085..4235
                     /locus_tag="O2692_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCZ4300137.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLQEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            4228..4683
                     /locus_tag="O2692_002537"
     CDS             4228..4683
                     /locus_tag="O2692_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCZ4300138.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
     gene            5330..5412
                     /locus_tag="O2692_002538"
     tRNA            5330..5412
                     /locus_tag="O2692_002538"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5363..5365,aa:Leu,seq:gag)
BASE COUNT         2069 a          677 c         1046 g         1640 t
ORIGIN      
        1 attatttcga tgggtatata ataatttgaa atataaatat ggtggataca gcgcttaaaa
       61 tgaagataaa tatttttaat aagtaggagt gtaatgaaat gaatcttaat atagaaacaa
      121 ccactcaaga taaattttac gaagttaaag tcggtggaga attagatgtt tatactgtgc
      181 ctgaattaga agaggtttta acacctatga gacaagatgg aactcgtgat atttatgtta
      241 atttagaaaa tgtgagttat atggattcga caggtttagg tttattcgta ggtacattaa
      301 aagcattaaa ccaaaatgat aaagaactat acattttagg tgtgtcagat cgtatcggta
      361 gactatttga aattactggt cttaaggatt taatgcatgt taatgaagga acggaggtcg
      421 aataacatgc aatctaaaga agattttatc gaaatgcgcg tgccagcatc ggcagagtat
      481 gtaagtttaa ttcgtttaac actttctggc gttttttcga gagctggtgc tacatatgat
      541 gatattgaag atgccaagat tgcagttagt gaagctgtga caaatgcagt taaacatgca
      601 tacaaagaaa ataacaatgt gggcattatt aacatatatt ttgaaatttt agaagataaa
      661 attaaaattg ttatttctga taaaggtgac agttttgatt atgaaacaac taaatcaaaa
      721 ataggtcctt acgataaaga cgaaaatata gactttttac gcgaaggtgg cctaggttta
      781 tttttaatcg aatctttaat ggatgaagtc acagtatata aagaatctgg tgtgacaatc
      841 agtatgacta agtatataaa aaaagagcag gtgcgaaata atggcgaaag agtcgaaatc
      901 agctaatgaa atttcacctg agcaaattaa ccaatggatt aaagaacacc aagaaaataa
      961 gaatacagat gcacaggata agttagttaa acattaccaa aaactaattg agtcattggc
     1021 atataaatat tctaaaggac aatcacatca cgaagattta gttcaagttg gtatggttgg
     1081 tttaataggt gccataaata gattcgatat gtcctttgaa cggaagtttg aagccttttt
     1141 agtacctact gtaatcggtg aaatcaaaag atatctacga gataaaactt ggagtgtaca
     1201 tgttccgaga cgtattaaag aaattgggcc aagaatcaaa aaagtgagcg atgaactaac
     1261 cgctgaatta gagcgttcac cttctatcag tgaaatagct gatcgattag aagtctcaga
     1321 agaagaagtg ttagaagcaa tggaaatggg acaaagttat aatgcgttaa gtgttgatca
     1381 ttccattgaa gctgataaag atggttcaac tgttacgcta ttagatatta tggggcaaca
     1441 agatgaccat tatgacttaa ctgaaaaacg tatgatttta gaaaaaatat tacctatatt
     1501 atctgatcgc gaacgagaaa tcatacaatg tacgtttatt gaaggattga gtcaaaaaga
     1561 gacaggtgag cgtatcggtt taagtcaaat gcatgtatca cgacttcaga gaacggcaat
     1621 taagaaatta caagaagcag cacatcaata gaatttgttt attaatgata cgttttataa
     1681 tgaaaaatcc atataattat cccttgatta ttaaattgaa atcgaggggt atttttaatt
     1741 taattaagat tttcgaatta atacattatt agtgtagttt aatgtgtatc cacataaatg
     1801 tcgcgatata gtattaataa tttaagtgaa gaagatatct aattgtcgtt ttaaataggt
     1861 gggttgctat tagaataaaa aaagtagtct tagattatga aatttagaaa tgatggtgtg
     1921 tcattttcaa taatcttagt gcgttttaaa atatagtatg acctaatcat tcgttttaaa
     1981 tgttttggaa gtgaaaatta cattaagtat cataccttaa tagaagtatt ttagaatatg
     2041 ttaaaataaa tgagtaaatt taagaaaaag tgtgggttaa gtaaatggac aatcaattga
     2101 ttaattcaat catagagaaa tatcaattta gtaaaaaaca aattgaagca gtattaacac
     2161 tgctagaaga aaaaaataca gtaccattta ttgcgaggta tcgaaaagag caaactggtg
     2221 gactagatga agttcaaata aagcaaattg atgacgaata ccaatatatg gtcaatttac
     2281 aaaaacgtaa agaagaagtt atcaaaaata tagaacagca aggattactt actgaggaat
     2341 taaagaagga tattttaaaa cagaacaaat tacaacgtgt tgaagaccta tataggcctt
     2401 ttaaacaaaa gaaaaagaca agggcaactg aggcgaaacg taaagggtta gagccattag
     2461 cgatatggat gaaggcacgt aaacatgaag tctcaattga agaaaaagca caacaattta
     2521 taaatgaaga agtgcaatcg gttgaagatg ctatcaaagg tgcacaagat attattgcgg
     2581 aacaaatttc agataatcct aaatatagaa caaaaatttt aaaagatatg tatcatcaag
     2641 gtgtgttaac tacatctaaa aagaaaaatg ctgaagatga aaaaggtatt tttgaaatgt
     2701 actatgcata tagtgagcca attaaacgca ttgctaatca tagagtttta gctgttaatc
     2761 gtggtgaaaa agagaaagta ttatctgtaa agtttgaatt cgatacgaca tcagtagagg
     2821 atttcattgc acgtcaagaa atcaatcata ataatgtaaa tcgcagttat attttagagg
     2881 cgattaaaga tagcttgaaa cgcttaattg tcccttcgat agagcgtgaa atccatgctg
     2941 atttaactga aaaagctgaa aatcatgcaa tagatgtttt tagtgaaaac ttaagaaatc
     3001 tattactgca acctccaatg aaaggtaaac aaatattagg cgtagatcca gcatttagaa
     3061 caggttgtaa attagcagtc attaacccat tcggtacttt tatagcaaaa ggtgtgattt
     3121 atccgcatcc accagtttct aaaaaagagg cagcagagaa ggattttgta caaatggtta
     3181 aagcgtatga tgtgcaatta attgcaattg gcaatggtac tgcaagtcgt gaaacagaac
     3241 aatttgttgc agatttaatt aaaaagcatc agttgccagt acaattcatc attgtcaatg
     3301 aagcgggcgc ttcagtatac tcagcatcag aaattgctag agatgaattt cctgattttc
     3361 aagtggaaga aagaagtgcc gtatcaattg gacgacgcgt tcaagatcca ttaagtgaat
     3421 tagttaaaat cgatcctaaa tctattggtg tcggacaata ccagcatgat gttaatcaaa
     3481 aagcactaga aaatgcatta actttcgttg ttgaaacagc agttaaccaa gtaggtgttg
     3541 atgtcaatac agcatcatca tcattattac aatatgtgtc tggtctaagt tcgcaaattg
     3601 ctaaaaatat aattgcatat agagaagaaa atggcgcaat aaagcataat aaagagttaa
     3661 gcaagattaa gcgtttgggt gctaaaactt ttgagcagag tataggattt ttaagaatag
     3721 tagacggttc agagccattg gataatacat cgattcatcc cgaaagctat aaagtaacgt
     3781 atcagttatt agataaactc ggatttggtg gcaatgattt aggtagtgat gcattaaaag
     3841 ctaaattaaa ttcattggat atggatgaac tggcgattga attacaagtc ggtgtaccta
     3901 cattagagga tattattaaa tccttgaaag cacctaatcg agatccaagg gacgagtttg
     3961 aaacaccaat ccttaaatcc gatgtgctat caattgaaga tttacaagaa ggaatgaaat
     4021 tgagtggtac agttagaaat gtagtagatt ttggagcatt tgtagatata ggtgttaaac
     4081 aagatggact tgtccatgtt tcgaaacttt ctaagaagtt cgtaaaaaat ccaatggata
     4141 ttgtcagtgt tggtgatatc gttgacgttt ggatttacag tattgacaaa aataaagaca
     4201 aagtatcatt aacgatgatt gatccacatg aataatgaca aacttcagcg aatggttgag
     4261 aatctttcgg aagaaaaatt tggacgtacg tttcgacatt gtgcatattt taataaaagg
     4321 ttacgtacaa caggcggacg ttatctttta aagtcccatg atattgaaat taatccaaaa
     4381 caatatgagc attatggaga agatgctgtt gtaaaaatta tactgcacga attgtgtcat
     4441 tatcacttgc atattgctgg taaaggttat caacataaag atcaagattt taaacgattg
     4501 agccaacagg ttggagcgcc tagattttgt aacagcattg aaagctatca acaacgggca
     4561 aattatgaat attattgcac taaatgtcat gcaaaatata tacgtatccg taaagttgat
     4621 acgaatcgta tgcgttgcgg acattgcaat ggaaaattaa gaatgaaaag acaattgaaa
     4681 taagtacaat acaaatagag ctaattcgtg tatatgtaag attatttatg agttgtatgg
     4741 aatttatata gaatttatca gtgagttatt ttgattggta aacatagtaa ttgaataaga
     4801 agaaattgtg tttataaaat gtttaaagcc gagattgtcg taatttaata cttcgcaatc
     4861 tcggcttttc tacttatgat ataggtaatg gcataaattg tggcttgata ggatgaaatt
     4921 aaaatgatta gattaaaaat cttggcgtat tttagtaata attaatatat aaactttacc
     4981 caattaaagc tttaaagtag cacaaaagat aattatacat ttttcatgac attttgattg
     5041 aacttttcta ttgactgtac agcttatagt tagtatgatt aaacacgttc cttaaaaaag
     5101 aacaaattaa ataaactttg atttgaaaga cagtcaattc taaatttaat ttataaaata
     5161 tgttgacaat aagttctaaa tgttttatta taaatagcgt tgtgttaaca actgataaca
     5221 caaattaatt taatgtaaaa aaacgattaa aagttattga caaatatttt taaacataat
     5281 ataataaata acgttctgaa aaacgaagat gttttaaatg cctatatccg cggtcgtggc
     5341 ggaatggcag acgcgctagg ttgagggcct agtgggagaa atcccgtgga ggttcaagtc
     5401 ctctcggccg catcaaaatt cttaatttaa at
//
LOCUS       DALAGF010000139         5336 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.140, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000139 DALAGF010000000
VERSION     DALAGF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.140"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            78..785
                     /gene="splA"
                     /locus_tag="O2692_002539"
     CDS             78..785
                     /gene="splA"
                     /locus_tag="O2692_002539"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HCZ4300139.1"
                     /translation="MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNIEK"
     gene            910..1632
                     /gene="splB"
                     /locus_tag="O2692_002540"
     CDS             910..1632
                     /gene="splB"
                     /locus_tag="O2692_002540"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HCZ4300140.1"
                     /translation="MNKNVVIKSLAALTILTSVTGIGTTLVEEVQQTAKAENNVTKVK
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            1690..2409
                     /gene="splC"
                     /locus_tag="O2692_002541"
     CDS             1690..2409
                     /gene="splC"
                     /locus_tag="O2692_002541"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HCZ4300141.1"
                     /translation="MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVK
                     DTNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQAFNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            2530..3249
                     /gene="splD"
                     /locus_tag="O2692_002542"
     CDS             2530..3249
                     /gene="splD"
                     /locus_tag="O2692_002542"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplD"
                     /protein_id="HCZ4300142.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENSVKLIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVTSDAVVQPGSSGSPILNSKREAIGVMYASDKPTGEST
                     RSFAVYFSPEIKKFIADNLDK"
     gene            complement(3198..3305)
                     /locus_tag="O2692_002543"
     CDS             complement(3198..3305)
                     /locus_tag="O2692_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300143.1"
                     /translation="MLILLLISKNHLSMYGWMIYLSKLSAINFLISGEK"
     gene            3407..4123
                     /gene="splE"
                     /locus_tag="O2692_002544"
     CDS             3407..4123
                     /gene="splE"
                     /locus_tag="O2692_002544"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HCZ4300144.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIK
                     NTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKV
                     KKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
                     NKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTK
                     GYGVYFSPEIKKFIADNTDK"
     gene            4274..4993
                     /gene="splF"
                     /locus_tag="O2692_002545"
     CDS             4274..4993
                     /gene="splF"
                     /locus_tag="O2692_002545"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HCZ4300145.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGEST
                     RGFAVYFSPEIKKFIADNLDK"
     gene            5058..5192
                     /locus_tag="O2692_002546"
     CDS             5058..5192
                     /locus_tag="O2692_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300146.1"
                     /translation="MHYLICIKANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI
                     "
BASE COUNT         2102 a          771 c          921 g         1542 t
ORIGIN      
        1 tttttattta aaatgtatat agtaaatgtg tatctagata cattaactat aaaaataaat
       61 ttggaaggag gtaaaatatg aataaaaatg taatggttaa aggtttaact gctttaacta
      121 ttttaacatc tcttggtttt gctgaaaata tttctaatca gcctcattca attgccaaag
      181 cagaaaagaa tgtcaaagaa attaccgatg caactaagga accatacaat tcagtggtag
      241 catttgtggg tggtactggt gtagttgttg gtaaaaatac aatcgtaact aacaaacata
      301 tcgctaaaag taatgatatt tttaaaaata gagtatcagc acatcattcg agtaaaggta
      361 aaggcggagg aaactacgac gttaaagaca ttgtagaata tcccggaaaa gaagaccttg
      421 cgatagttca tgttcatgaa acaagtacag aaggtttgaa ttttaataag aacgttagtt
      481 atacaaaatt tgcagacgga gcaaaagtga aagatagaat ttctgttatt ggttatccaa
      541 agggtgcaca aacaaaatat aaaatgtttg aatcgacagg aacgattaac catatcagtg
      601 gaacgtttat ggaatttgat gcgtatgcac aaccaggtaa ttcaggatct cctgtattga
      661 attctaaaca tgaactgatt ggtattttat atgcaggtag tggaaaagat gaatctgaaa
      721 agaatttcgg tgtttatttc acaccacaat taaaagaatt tattcaaaat aatattgaaa
      781 aataagtatc atcaattcat tcgtgaagtt gattttttaa agagtgatta agaaaacggt
      841 tacataatta actaaatata ctgaattatg tatctagata tcaaaataat taaaagagag
      901 gaacttaaaa tgaacaaaaa cgtagtcatc aagagtttag cagcattaac aattttaaca
      961 tctgtaacag gtattggaac aacattggtt gaggaagtac aacaaactgc caaagcagaa
     1021 aataatgtca caaaagttaa agatactaat atttttccat atactggtgt agttgctttt
     1081 aaaagtgcaa ctggatttgt agttggaaag aatactattt taacaaataa acatgtgtcg
     1141 aaaaattaca aagtgggcga tcgtattact gcacatccaa atagtgataa aggtaatggt
     1201 ggtatttatt cgattaaaaa gattattaat tatccaggta aagaagatgt atcagtcatt
     1261 caagttgaag agcgtgcaat agaacgtgga ccaaaaggct ttaattttaa tgataatgta
     1321 acgccattca aatatgcggc aggggctaaa gctggtgagc gaattaaagt gattggttat
     1381 ccacacccat acaaaaataa atatgtttta tatgagtcaa ctggccctgt gatgtcagta
     1441 gaaggtagca gtattgtata ttcagcgcat actgaaagcg gaaactctgg atcacctgta
     1501 ttaaacagca acaacgaatt agttggtatt cattttgctt ctgatgtaaa aaatgatgat
     1561 aacagaaatg catatggcgt ctactttaca ccagaaatta aaaaattcat tgcagaaaac
     1621 atagataaat aaacaaattg actttaaacg agcgttgcaa catatctcga attgtaaagg
     1681 agcttgaaaa tgaataaaaa tatagtcatt aaaagcatgg cagcattagc cattctaacc
     1741 tcagtaactg gaataaatgc tgcagtcgtt gaagagacac aacaaatagc aaatgcagag
     1801 aagaatgtta cgcaagttaa agatacaaat atttttccat ataatggcgt cgtttcattt
     1861 aaagatgcga caggttttgt aattggaaaa aatacaatta tcaccaataa acatgtatca
     1921 aaagattata aagttggcga tagaattact gcccatccaa acggtgacaa aggaaatggt
     1981 ggtatatata aaattaaaag catttctgat tatccgggtg atgaagacat ctctgtcatg
     2041 aatattgaag aacaagcagt cgaacgtgga ccaaaaggct ttaattttaa tgaaaatgtc
     2101 caagcattca attttgcgaa agatgctaaa gttgatgaca aaattaaagt tattggttac
     2161 ccattacctg ctcaaaatag ttttaaacag tttgaatcta caggaactat aaaaagaatc
     2221 aaagacaata ttttaaattt tgatgcatac attgaacccg ggaattcagg atcaccagtt
     2281 ctaaattcta acaatgaggt cataggtgtg gtgtatggcg gtattggaaa aattggttct
     2341 gaatataatg gtgccgtata ctttacgcct caaatcaaag attttattca aaagcacatt
     2401 gaacaataaa caaatttaaa tatacaccat gagcatgtgt tcaataattt taatgaaaaa
     2461 catcggtcga atataacata aaaaaacgtc tatatcaaaa gcatcatgaa taaacagagg
     2521 agcacaaaaa tgaataaaaa tataatcatc aaaagtattg cggcattgac gattttaaca
     2581 tcaataactg gtgtcggcac aacagtggtt gatggtattc aacaaacagc caaagcagaa
     2641 aatagtgtga aattaattac caacacgaat gttgcaccat acagtggtgt tacatggatg
     2701 ggcgctggaa caggatttgt agttgggaat catacaatca ttaccaataa acatgttact
     2761 tatcacatga aagtcggtga tgaaatcaaa gcacatccta atggttttta taataacggt
     2821 ggtggacttt ataaagttac taagattgta gattatcctg gtaaagaaga tattgcggtc
     2881 gtacaagttg aagaaaaatc aacgcaacca aaaggtagaa aattcaaaga tttcactagc
     2941 aaatttaata tagcatcaga agctaaagaa aatgaaccta tatcagtcat tggttatcca
     3001 aatcctaatg gaaataaact acaaatgtat gaatcaactg gtaaagtact atcagtgaat
     3061 ggaaatatag tgacatctga tgcggttgtc caacctggca gctctggttc acctatatta
     3121 aatagtaagc gagaagcaat tggtgttatg tatgctagtg ataaaccaac aggtgaaagt
     3181 acaaggtcat ttgctgttta tttctctcct gaaattaaga aatttattgc agataattta
     3241 gataaataaa tcatccatcc atacattgat aaatgatttt tagaaattaa caacaaaatc
     3301 aacaatttta aacatctctg tgattctatt tattcgaaat gatttaaaaa ataaaacttc
     3361 aaaaacctaa ccttatattt atacgaatac ttagaggagc acaaaaatga ataaaaatat
     3421 aatcatcaaa agtattgcag cattgacgat tttaacatca gtgactggcg tcggcacaac
     3481 agtggttgag ggtattcaac aaacggctaa agctgaacat aatgtgaaac taatcaaaaa
     3541 tactaatgta gcaccataca atggtgtcgt ttcgatagga tctggaacag gtttcattgt
     3601 cggtaaaaat acaattgtta ccaacaagca tgtcgttgca ggtatggaaa ttggtgcaca
     3661 tattatagcg catcccaatg gtgaatataa taatggcgga ttttataaag ttaaaaaaat
     3721 tgtccgttat tcaggtcaag aagatattgc cattctacat gtggaagata aagctgttca
     3781 tccaaaaaac aggaatttta aagattacac aggcatttta aaaatagcat cagaagctaa
     3841 agaaaatgaa cgcatttcaa ttgttggcta tccagaacca tatataaata aatttcaaat
     3901 gtatgagtca acaggaaaag tgctgtcagt taaaggcaac atgattatta ctgatgcttt
     3961 cgtagaacca ggcaactcag gttcagctgt atttaacagt aaatacgaag ttgtaggtgt
     4021 tcactttggt ggaaacggcc ctggaaataa aagtacaaaa ggatatggtg tttatttctc
     4081 tcctgaaatt aagaaattca ttgcagataa cacagataaa taaatcctta catagataaa
     4141 tgattttaaa aattaacaac aaactcaaca attcaaatca tctctgtgat tccatttatt
     4201 cgaaatgatt aaaaaaaata aaacttcaaa aagctaacat tataattata caaatactta
     4261 gaggagcaga aaaatgaata aaaatataat catcaaaagt attgcagcat tgacgatttt
     4321 aacatcaata actggtgtcg gcacaacaat ggttgaaggt attcaacaaa cagccaaagc
     4381 cgaaaatact gttaaacaaa ttacaaatac aaatgttgca ccatacagtg gtgttacatg
     4441 gatgggcgct ggaacaggat ttgtagttgg aaatcataca atcattacca ataaacatgt
     4501 tacctatcac atgaaagtcg gtgatgaaat caaagcacat cctaatggtt tttataataa
     4561 cggtggtgga ctttataaag ttactaagat tgtagattat cctggtaaag aagatattgc
     4621 ggttgtacaa gttgaagaaa aatcaacaca accaaaaggt agaaaattca aagatttcac
     4681 tagtaaattt aatatagcat cagaagctaa agaaaatgaa cctatatcag tcattggtta
     4741 tccaaatcct aatggaaata aactacaaat gtatgaatca actggtaaag tattatcagt
     4801 gaatgggaat atagtgtcat cggatgcaat tattcagcct ggtagctctg gttcacctat
     4861 attaaatagt aaacacgaag ctattggtgt aatctatgcc ggtaataagc catcaggtga
     4921 aagcacaaga ggatttgctg tttatttctc tcctgaaatt aagaaattca ttgcagataa
     4981 tttagataaa taattaaaac ttagacattc acccaatcct gacaaaatat actataacta
     5041 acatttatta atatatattg cattatttaa tatgcatcaa agccaatcaa cgattgattt
     5101 tcaccaactc aattgttgat tggttttatt tatgtatgaa tgaacaactt tttgacatca
     5161 ttaagaatat aaatgatttt gaaagcattt gaaagctaca acatttctat aaaatttttc
     5221 aataacaatt gcgccactaa aactcaaaat ttccaccacc aacatccaaa ttatcaacat
     5281 cgcaacataa ccaaatgtta taataaatct attacacaaa gagataaatt acttat
//
LOCUS       DALAGF010000140         5299 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.141, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000140 DALAGF010000000
VERSION     DALAGF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.141"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(390..1307)
                     /locus_tag="O2692_002547"
     CDS             complement(390..1307)
                     /locus_tag="O2692_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCZ4300147.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            1398..1499
                     /locus_tag="O2692_002548"
     CDS             1398..1499
                     /locus_tag="O2692_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300148.1"
                     /translation="MVINEIDFYRVGRENIVIAVYNEHIKFLFILIS"
     gene            complement(1577..3082)
                     /locus_tag="O2692_002549"
     CDS             complement(1577..3082)
                     /locus_tag="O2692_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCZ4300149.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAISPLIIKAMKGIR"
     gene            complement(3423..3923)
                     /gene="queF"
                     /locus_tag="O2692_002550"
     CDS             complement(3423..3923)
                     /gene="queF"
                     /locus_tag="O2692_002550"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCZ4300150.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            complement(3943..4809)
                     /locus_tag="O2692_002551"
     CDS             complement(3943..4809)
                     /locus_tag="O2692_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCZ4300151.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
BASE COUNT         1865 a          835 c          823 g         1776 t
ORIGIN      
        1 gtgtaactca tgcattcata tttttaagta cacattagct gtgactaatg ataaagaatc
       61 gctacataat caatcattag tcgttcttta tcatttccgt cccgctctca ataaatgtta
      121 gtttatctta ttattataaa tcggatgaat gtgttaatct atggcagatt acacgtcatc
      181 cgatttttta tagaatttga aaaagacgca taaaccacta tgatttaaaa tacaacatca
      241 atcattttag tggcatgcgc caaaattata tgtctgtttt tgaaacaggg taatagctta
      301 aagctaataa aaacgaatat aaggtgcgtt gaatcttatg attacactcc aaacctaata
      361 taatatcggg ttaagatcat tccggatgct tacaaatcat tgacagtaag taactgaatg
      421 gcatttggta taacctcaat atcaataggt gtttctaatg aaatttcgcc atcaatatca
      481 actttcattg ctggatctgt tgtaagtgaa atctttttac caggtatatg ctcaatacct
      541 tgagtaattt cattccaatt catgctatca cgctttttaa aaatatcatt taaaatactg
      601 aaactttgtt cattaaaaat gaaagtgttc agttcaccat cttgaggaga caaatcagtc
      661 aatggtatac gactaccacc aatgaatgga ccatttgctg ttagtatcat ggtcgtttcg
      721 ccagaatatg tcttatcatc tattgataat tgataattaa attgtgttgg atttagcagt
      781 gttttgacag ttgatccaat ataactcaat ttaccaaata tatcttttga accatcttgt
      841 acgttttcag cgttttgaac aatgagacct aagccaacaa agttgagtgc atattgatta
      901 tttattttaa ttacatcgta tgtaccaact tgtgcagaaa tcatttgttc actagcttgt
      961 ttatgattag gtgctatatt tagcgttttt gtaaaatcat taaaagtacc gcctggtaaa
     1021 atgccaatag ggagttgaag gtcatgtgtc ataacaccgt ttataagttc gttaaccgtg
     1081 ccatcaccgc caagaataaa taatatatct acatcttttg catagttttt agttttgatt
     1141 tcttggcaat atttaataat gtcaccttcg ttttcactca attgaataga aagatgctta
     1201 caaattgaac ttaatgctgt tgtaacttcc ccaatacctt gattaatatt ttttaatcca
     1261 ctgtgttcat ggtaaaagag gacaccatgt gtatatttat tttccatagt ttagcctact
     1321 ttctaaaaat tggttcatta aatatatata cccactttta attgttaata ccaaaaatat
     1381 gtttttaaat agagaaaatg gtaataaatg aaattgattt ctatagagtg ggacgagaaa
     1441 atatagttat agctgtctat aatgagcata ttaagttttt atttatactg atatcttgaa
     1501 tttaattaat agaaacctat aaaaaaacag taagccattt aaatgactta ctgttttttg
     1561 aattaggcca acaatattaa cgtatacctt tcatcgcttt gatgattaaa ggtgagattg
     1621 ctaatacaat tgttgtaaca ataattgcaa caacacctag gaaaataaag taatttgttt
     1681 gacctagtgg ttctattaac ttaactaaag taccattgat tgcttgtgca gaagcgttag
     1741 ttaagtacca aatactcatc atttgggcat taaatgcttt aggtgctaac ttaacagcag
     1801 cactattacc cgttggtgat aagcatagct caccgataac acaaataatg tacgataaaa
     1861 taacccagtt aactgaaaag tttgatgaac ctgatgcata acctacaata ccaattagta
     1921 tgtatgacgc acctgctaag aacgtaccaa ttgcaaattt tactggcagg ctaggttgtt
     1981 tagttccaag cttttgccat aaaagtgaaa taattggagc tagtaataaa ataaataatg
     2041 ggttaattga ttggaagatc gcttcaccaa agtttgtttt ccaaccaaat aagtttaatt
     2101 tcatatctga atgttcaatt ccatatatgt ttaatacatt agacccttgt tcttgaatag
     2161 cccagaacac cattccaaga ataaataatg gaataaatgc tttaacacga gaacgttcag
     2221 tatcagtgac atctttactt ctaataatta aagtgaagta aatgattggt aatgcaatac
     2281 ctaatactaa aacagtatta ctaactaagt taaatgataa tgagttagtt aatgcaccaa
     2341 taacgataat taatacaatt gctaaaacaa cacttccgat aataagacca tactttttct
     2401 tttcagctgg tgtcaatggg ttagtaggtt tcataccaac gctacctaag tttttgcggt
     2461 tgaaaagtac ataccatact aaacctaatg ccataccaac tgctgcaatc aagaatccgc
     2521 cgtggaagtt tttaacatta acaaagtgtt gcaaaataat aggtgataat aatgcaccca
     2581 tattaactga catatagaaa ataacaaaac ctgcatccat acgtctatca ttttcaggat
     2641 ataaacggcc aacgatattt gaaatgtttg gcttcattaa acctgaacca ataatgatga
     2701 agaacattga tgtgaataag ccgattaatg caaatggtaa gcttaaacaa atatgtccga
     2761 taataataaa gactgcacct aataaagtag cgcctctagt gcctgtaatt ctgtcagcaa
     2821 tccatccgcc tggtattgat gtcatataga ttaatgaacc ataaactgac ataattgaca
     2881 tagctgttgt tttatcaatt ccaaggccat tatctgttac ggcaaagtac atgtagaaaa
     2941 tgagtagggc acgcatgcca taataactaa acctttccca gaactctaca aagaagagta
     3001 cgcctagtcc tcgaggatgc ccgaaaaatc ctgtttgagg tatgtcttga atttgatttc
     3061 catgggagtt ttgttgtgtc atgtatacat cccatccttt cttcccctaa tacaatagtt
     3121 taagtaaatt tgattgtgcc ttatcaacta aatattccaa caaatcacaa aaaaataaaa
     3181 atattgagaa tattctgtta ttaagcgcgc acaattaatt agagataata taaaaatata
     3241 cttattgacg ttaaagtgca ataggtttct ataaaaatgt tgtttaattc gtgtactaat
     3301 attcttttgg tgtactttgg tataaaatgt tattttaatg taacctatta tgtatgctaa
     3361 atgaaaaaca caccaataag ttaggaaaat caacttattg gtgtgtttat taaatgatac
     3421 atttaacgat tatctatttt ttcgggatat aaatcatgat tcatcaaacg atgctcagcc
     3481 attttttcat atttagaatt tggacgtcca tagtttgtat aaggatcaat agaaattcca
     3541 ccacgtggtg tgaacttgcc ccagacttca atataatgtg ggtccataag ctctatcaaa
     3601 tcattcataa taatattcat acaatcttcg tgaaaatcac cgtgatttct gaaactaaat
     3661 aagtataatt tcaaagattt tgattcaacc attttaacat ttggaatata tgaaatatag
     3721 atagttgcaa aatctggttg cccagtaatt ggacataatg atgtaaattc tggacagttg
     3781 aattttacga aatagtcacg accttgatgc ttattatcaa acgattctaa tacatcagga
     3841 cgatagtcaa aattgtaagt attgtcttga tttcctaata aagttatatc ttgtaattca
     3901 tcttgttgac ggccatgtgc catataaagc gctcctttaa atttattttt ttattatttt
     3961 ggcgtctcgg cgtgcttttt caaacatgta ataacttgca ccgataataa cgacgtaacc
     4021 taatgttgca tagaaatctg gagattctcc gaatagaata aatccaagta ttgctgtgaa
     4081 aattatagat gcatacgtaa aaatagaaat atcttttgct gctgcaaaac tatatgctaa
     4141 agtaacacca atttgaccca cagcggcagc taagccagcc cctaatagat aaagtatttg
     4201 catctgactc attggttcat aagtatatgc agtgaaaggt attaaaacga tgacagaaaa
     4261 taaggagaag taaaatacta tagtatatgg tgcttctctt gtactaagtg ctcgaacaca
     4321 tgtatatgct gatgctgcaa aaatacctga gaataagcca gctaatgatg gaatcataga
     4381 tgatgaaaat tcaggtttca ctattaaaag catacctaaa atagcaatta tcattgctgt
     4441 aatttgatac ttccttacct tttcatgtaa gaaaacaatg cttaataaaa tcgtccagaa
     4501 aggattgagt ttcattaatg aatcggcatc actaagtacc atatgatcaa tggcataaat
     4561 atttaacaat acaccaataa gtccaagtgt tgatcgtgtt attaataagg gttgacttga
     4621 aagtctgcca aacattggct gatggtattt atatataaaa aataatggaa taaacattgc
     4681 tactaagttt cgtgctaatg atttttgaaa aacaggaagg tcacctgcaa gtctgaaaaa
     4741 cactgacata aaactgaaac caatagccga aattaaaatg gcaatgatac cttttacttt
     4801 aggattcaat tttatcgcct cttttatata aaattaacgt atttatatta gcataaaaca
     4861 acatgttgtg cataaatagt tgaaatttac tataaaaaga ctataataga ctgtagcgaa
     4921 caaacgttct gtgtttattt gtcggaataa tagggcatta cacttttatg aatgtttgtg
     4981 ttattacata aaacaaatat caattcagta tcaagctaat aagctttttc ttgatttctg
     5041 ttgatacaat tgagattgac acagatttaa aaaaatcaag tgatatctac taaaaaattt
     5101 ttttaaattt gttcaagttt ttctaattta gtattggtgc ctagttggaa cgttttacga
     5161 acattcgatt agaaaatggc actttaaatc atagtgtgtc ttatgtataa tgaaacacat
     5221 aatatagtgt tggtgaaacg aaaaagacac aatatcttgt gttttgtatg caaatgcttt
     5281 atttatgaag aaattacat
//
LOCUS       DALAGF010000141         5289 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.142, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000141 DALAGF010000000
VERSION     DALAGF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.142"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(150..1004)
                     /locus_tag="O2692_002552"
     CDS             complement(150..1004)
                     /locus_tag="O2692_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCZ4300152.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(1280..1504)
                     /locus_tag="O2692_002553"
     CDS             complement(1280..1504)
                     /locus_tag="O2692_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300153.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
     gene            1703..2173
                     /gene="sigS"
                     /locus_tag="O2692_002554"
     CDS             1703..2173
                     /gene="sigS"
                     /locus_tag="O2692_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HCZ4300154.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRL
                     QDIFKLLNQRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN"
     gene            2286..2730
                     /locus_tag="O2692_002555"
                     /pseudo
     CDS             2286..2730
                     /locus_tag="O2692_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
     gene            complement(2717..3160)
                     /locus_tag="O2692_002556"
     CDS             complement(2717..3160)
                     /locus_tag="O2692_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300155.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMFASIYFIKIVTSYFTKKLLEIKFNSK"
     gene            3456..4091
                     /gene="sdpA"
                     /locus_tag="O2692_002557"
     CDS             3456..4091
                     /gene="sdpA"
                     /locus_tag="O2692_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HCZ4300156.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIVVSEVHL
                     LYSMLIVLVGLILLRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPAN
                     EQQLEQAIRNTPFYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLIVSSLVFAS
                     LHESDTWIGYLPYLYSGLIFGLIYIKTKRLEVVIFMHFLNNLLALLFIIWR"
     gene            complement(4531..5244)
                     /locus_tag="O2692_002558"
     CDS             complement(4531..5244)
                     /locus_tag="O2692_002558"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCZ4300157.1"
                     /translation="MAKLNIEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
BASE COUNT         1767 a          883 c          645 g         1994 t
ORIGIN      
        1 ttctttatcg atgtcctgcc ccacttgcat tggtgggatt atcaatcaat gtgttttctc
       61 taatgcaaaa agacgctttc tatcaaaaca agatagaaag cgtcttttta gtatgtccga
      121 gtcataaaaa caactcgtag cttatcaaat tacttattca aatgtttact gtcatcttta
      181 tacacaaagt atttgaagta ttttccttca gtcttcatgt tcttataaaa gtcagcgata
      241 attgttgcat tactttctgc ccacttaata tctgtagcat attgatgttc tcctggattt
      301 tttggattcc atctcatact atacaatgta ttttgatctg tgcttgataa gaagtgctta
      361 tgaatgaaat cagcaccgcc tgaaatagct ttttcaggtg tatcccaacc atgcttttta
      421 gcatattctg cacctgtttt aattgggtct ttatcaaggg ctcctactcc atagaaattg
      481 tagtactttt tgccatcaat ttcgactcca ttagctaatt cacttttaac tgcgccagtt
      541 tctaataatg catgtgaaat taaataaact tcgttaacgt gcttatcttt agcagctttt
      601 aagaaatcat ccgtatgttt caataacgtt ggtctatcta ctaacatacg tttaattcta
      661 tttttatcaa tcccttgata ctttgataaa tctaaaaatt gatatttttg cttttcatta
      721 tcgataaaag taccgctatc cattgcactt ttaatttcag ttgcagatgc atctctccat
      781 gcatcattct ttttatttga tacctgttga ctcgtataat tatttatttg tttctttgct
      841 gcatcgttta atgtaacatt taacttttca atcttaatgt cggatttaac atgtttgaaa
      901 aatatctgat cggatatcat tgagaaaaat aaaaatgaga caacagcaaa tatgacaaca
      961 agtcctatta ttccaaaaat agaacctttc ttgtgtttat tcatatccac acctcttagg
     1021 tcattgttgt tatacattca gtttgactac gataataaag tttaacatta ttaaaaccgt
     1081 ttaacaattt tatctatgca ttgttacata ttcataaata aacagtaatg taaatgaaac
     1141 gttttaaacc atttatcaac attactttac gttgatctac gaatcataaa tgttttatct
     1201 ttttgtataa cctttgaaaa cctttatatc aataatctta ttattcacgt attaaaaaac
     1261 aaaaaattct tttgttcgtt tacttagttt cttttttata atccatcata ataaaacttg
     1321 ctgcatttga tctattttta taatagtact gatttaaatc aatcgtgcct tcaatttgac
     1381 catcacgata catcatttca ttagtgaagt tatttatcat cacgaaatca gcgcgatctc
     1441 ttataacatc taaatcgtcg tttcgtgcaa aaaaatgttc taaatttaaa tgtgcgtaat
     1501 ccattgttac ctcccgatta caaattttgt tctatctact atttagcaaa gtaataatac
     1561 atcatatcac attaaattaa ttacttatgt cttatattta acacttaatt tagcgattac
     1621 gcaaatgaat gatttttcta aatattttat tctaacttta tccggaaata tttgttatac
     1681 gtataacttg gagaatgact acttgaaatt taatgacgta tacaacaaac accacaaaat
     1741 catacaccat cttttaaaaa aatataatat tagctataat tatgatgagt attatcaact
     1801 actcttgata aaaatgtggc aattgagtca gatatataaa ccctcaagca agcaatcttt
     1861 atcctctttt ttattcactc gattaaattt ttaccttatc gatttattca gacaacaaaa
     1921 tcaattaaaa gatgtcattt tatgtgagaa taattcacca acattaactg aacaaccaac
     1981 atactttaat gaacatgacc ttcgtttaca agatatcttc aagcttttaa atcaaagaga
     2041 aagactatgg ctcaaactat accttgaagg atacaagcaa tttgaaattg ctgaaatcat
     2101 gtcattatcg ctttcaacga ttaaattaat taagatgtcc gttaagcgta aatgccaaca
     2161 taattttaat tagtttattg tgaaatttcg gtcgatgtgt taatgatgtt gaaacaccct
     2221 aacatcttca tataaattta ttttaaccat taaattaaca acttattaaa cgaggagtac
     2281 tatatttgca agacaattct actaaatatc tactttatat ccaaactgct acttcaaacc
     2341 atctcgaaac aaattgtgtc tttttacatt gcgattacat tcttaaagtt ccaattaaca
     2401 aactcgtttc atattatgcg aaattgcatt tatcatcaca aagtgtgcta attgagactg
     2461 caaaaaaaca tactaaatat taataaactg gttcctattt acatcaaccc aaaaactata
     2521 ctttttccgt taaaacataa acgtgcaccg atacaaattt atatcaatgc acattatatt
     2581 gttggtatga ctgccataga gaattcaaca ttaatacatt ttcaagaagg cattcagcta
     2641 gaagttgatg agccattttc tctagtttca aaaaaatgtc atgaaagttt agctttgaag
     2701 cacttcattg aaaatactat ttcgaattaa atttaatttc taaaagtttc ttagtaaagt
     2761 atgatgtcac aatttttata aaatatatgc tagcaaacat taataaaatc gctccaataa
     2821 ttgttaaaat aacaacagtt gtagataaat ctaccttagg actaacgaaa aattgtacgc
     2881 cagatagact tgtaattagc ccaaataaaa acaaacctaa taaagcaaca taagcaaaca
     2941 tcatcactac taatgtaaca aacatactat aactttttgt ttctttattt cgaaatacat
     3001 tttgtcttaa gtaagcatta gcaaaggtaa caggcttatt taagttaacg ctgtcatcat
     3061 cagatataga agacaatcgt tgatctaatg cttgtttctc cttcttgttc aaaaaccata
     3121 attgcttttt aactttttta tcaaatgatg ctttattcat aattaaggat ttctctcctt
     3181 tataatattg acgtaatttt aaacattagc gtcaattgaa agtgtatttt atatatttta
     3241 cagaaatata tgtatcaaat ctacaaaaat ccatattaca atttcattta tggtgatttg
     3301 acgctaaaat acagtctgct aatttttcaa actaatgctt tttaggtagg tacaatgata
     3361 aattttaatc gtaaatatgt ttcatatatt atgaatctag ccttaatcat attgcattca
     3421 aaataatttt aaaaaatgaa aagaaaggat ttagcatgca aaaattcaaa gacttttttt
     3481 acgatgattt atcggttaca cgaggaaatt attttttaac tttaatggca gcatttttta
     3541 ttactatcat tttatttatc ggcatagttg tcagtgaagt acatttactt tatagcatgc
     3601 taattgtatt agtaggttta attctattga ggctattcaa aatcaattta ttctctttta
     3661 aaaaattaac attgtctcaa gttatttata ttataggcgg tgcactatta atttatgggt
     3721 tagataatct ttatttatat tttcatgacg taccggcaaa tgaacaacaa ttagagcaag
     3781 caatacgaaa tacaccattc tatatttcta ttttcactgt taccatcatc cccgctattg
     3841 tggaagaaat tgtttttcgc ggtatgataa taagggttat cttcagaaaa cacttgtttt
     3901 tagggttaat tgtgtctagt ttagtttttg catcattaca cgaatctgac acttggattg
     3961 gttatttacc ttacttatat tctggtttga tttttggcct aatttatata aaaacaaaac
     4021 gattagaagt tgtaatattc atgcacttct taaataactt gttagctctg ctctttataa
     4081 tatggagata attttgagat aacctttcat attattttca aattatctat ctgatattta
     4141 ttttattgaa cttgagtcac cacttaaact caaaaatacg tgtcatcaag tgcaaaataa
     4201 tttagaacta atacaaacat aaaaactttt actttacgct taaaatcaaa agtcacatac
     4261 ctagatataa gtacaacttg tatattttgt actctaatag gtatgtggct taaataatat
     4321 ttaattcttg agttgcatat agtaactgtt tttattcatt ttctaaaata tgcaagacta
     4381 atttcctttt ctactcctgt tgaatcaatt tagacttttt tataactcga gcttttactt
     4441 agctgaattt tgacttcttc ttcattaata actatgatgg aaaaaccccc ataagcacaa
     4501 tgcctatgag ggtttaaata tatttatata ttatagaata gaaagacctg aagattgaat
     4561 atctttcgca aagcctttaa ctgtatctac tgataattcg ttaatatcgc gacctaagtt
     4621 tgtattcact tttttcacaa catctgctgg gcatgtaata atatctgcac caatttcatc
     4681 agcttgaatc acattgaata attcgcggca acttgcccat aataatttaa cgccgtcttt
     4741 actatgcgta acttttacag cctctttcat taatggtaat ggatctacgc ctgtatctgc
     4801 aatacgtcct gcaaatactg aaacatatgt tggcacacct tcagttactg cttcagttat
     4861 ttctttaact tgttcaattg tgtaaacagc cgtaacgttt aatctcacat tgtcagctga
     4921 aagtttttta attaaaggaa tcgttgattc accttttgta tttacaatag gaattttaac
     4981 aaatacattt tcgccatatt gttttaaaat tgctgcttct ttttccatag tttctaaatc
     5041 gtctgcaaat acttcaaatg aaattgaagc atctggaatt tctttcacag cttcttcagc
     5101 aaaagctttg taatctgtta cgcccgcttt agccattaaa ctaggatttg ttgtaaaacc
     5161 atccacttgt ttgtttttat aagctgcttt catttcttca atatctgcac cgtccgcaaa
     5221 tacttctata tttagtttag ccatataata tagcctcctt gattcttatt aaaattttaa
     5281 caacatctg
//
LOCUS       DALAGF010000142         5288 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.143, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000142 DALAGF010000000
VERSION     DALAGF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.143"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            64..1554
                     /locus_tag="O2692_002559"
     CDS             64..1554
                     /locus_tag="O2692_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HCZ4300158.1"
                     /translation="MHRESKYIEYKKSRKGLSNDIWSTYSAFANTEGGTIYLGIEEKK
                     IEDKKVFVSVGVEDPEKMIEDFWNALYGRSKVSQNILSNKDVKIVNIENKACIEIHVP
                     EAPYSKKPIYVDNKKDLVYKRVDDADRIATEEEYKFMIVNSQDDIDTELLDNYDMSDL
                     NHESIENYRKLLLKNTNDERYANMSQLDLMIDLGAYRKDRSSKDKQYKMTTACLLFFG
                     KYNAISDRFPGFQLDYFKKTNYLDTDWKDRISSGDLGNEDLNVYSFFEKVLIKLTDNI
                     EESFSLNDGLTRQNYARDLKVAIREALVNTLMHAYYDTKQSIKIVNCEDFIEFYNPGN
                     MRINKEDFIHGGHSKDRNSILSTLFRRVGYSEKAGSGGPRIFDVVNRHKLKTPEIELT
                     DMDTNVVLWKQDLMKEFEKYPELDKKVIKYIIDYGSISKGEALKMENMTEYQFRNILK
                     KLKDDNLIKKEGEGPATKYVLIESKEADILRTKKVIKSLESFFRNK"
     gene            complement(1927..3245)
                     /gene="cstB"
                     /locus_tag="O2692_002560"
                     /pseudo
     CDS             complement(1927..3245)
                     /gene="cstB"
                     /locus_tag="O2692_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002488497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
     gene            3301..3438
                     /locus_tag="O2692_002561"
     CDS             3301..3438
                     /locus_tag="O2692_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300159.1"
                     /translation="MELKLLNKLDDDQLDLEFLQDNGLKGNVQGPMTLKEPLKSYIQK
                     S"
     gene            3435..3806
                     /locus_tag="O2692_002562"
     CDS             3435..3806
                     /locus_tag="O2692_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCZ4300160.1"
                     /translation="MKTNILNLDNELENTEYVEGKPYVQYGIKYERNQALRNEAIKIH
                     GTTCKVCGFDFKAKYGDLGEGFIEIHHLKPMFSIKREIKVNPQKDLVPLCSNCHKMIH
                     RNTKKPLTIKELTKIVNYNSK"
     gene            3935..4555
                     /locus_tag="O2692_002563"
     CDS             3935..4555
                     /locus_tag="O2692_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300161.1"
                     /translation="MKFTEVEVIEHLVKAYKEAGKPTYPHENLYRGRNHSISGIGEDL
                     LGAYLISRLEGVQIFIDQPLSMIDKSLSTRYPDLLICEDNEIKNILEVKMDLGYQRKD
                     FIDYCRKKEEWISNIVGKQCVLSRKREDKIPMNIADDIKFHVVIYSENNGPKRFDEEI
                     MPIVNETCPHIEVYVLTSGQHPNLVNVNLEGININKDEFEILVNAL"
     gene            complement(5083..5217)
                     /locus_tag="O2692_002564"
     CDS             complement(5083..5217)
                     /locus_tag="O2692_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300162.1"
                     /translation="MKLHWYIYYFLSISVILFMIIYHYLATSMTVDYEITVSIAKLLL
                     "
     gene            5181..5273
                     /locus_tag="O2692_002565"
     CDS             5181..5273
                     /locus_tag="O2692_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300163.1"
                     /translation="MTKNSKYTNEVSKEQFQEIENVNNKVKEFY"
BASE COUNT         1991 a          634 c          916 g         1747 t
ORIGIN      
        1 tatggtagac ggtttgagta aattaatgga gtattttaat atttatggtt tggaggaatt
       61 gcgttgcata gagaaagtaa atatatagag tataagaaat cacgaaaagg attatctaat
      121 gatatttggt ctacgtatag tgcttttgca aatactgaag gtggtactat atatttagga
      181 attgaagaaa aaaagatcga ggacaaaaaa gtctttgttt cagttggtgt tgaagatcca
      241 gagaaaatga ttgaagattt ttggaatgca ctatatggaa gaagtaaagt tagtcaaaat
      301 attttatcaa ataaagatgt taaaattgtt aatattgaaa ataaagcgtg cattgaaatt
      361 catgtaccag aagcgcctta ttcgaagaaa ccgatatatg tagataataa aaaagattta
      421 gtatataaaa gagttgatga tgctgataga attgcgactg aagaagagta taaattcatg
      481 attgtaaatt ctcaagacga tatagataca gaattattag ataactatga catgtctgat
      541 ttaaatcacg aatctatcga aaattatagg aaacttctat taaaaaatac taatgatgag
      601 agatatgcga atatgagcca actggattta atgatagatt taggagcata tagaaaagat
      661 agaagttcga aagacaaaca gtataaaatg actacagcat gtttattatt ctttggtaag
      721 tataatgcga ttagtgatag attcccagga tttcaattag attattttaa gaaaacaaat
      781 tacctagata ctgattggaa agatagaata tcaagtggag atttaggtaa tgaagattta
      841 aacgtgtata gtttttttga aaaagtattg ataaaattaa ctgataacat tgaggaatca
      901 tttagcctaa atgatggttt gactagacaa aattatgcaa gagatttaaa agtagcaatt
      961 cgcgaagcac tggttaatac attaatgcat gcgtattatg atactaagca aagtattaaa
     1021 atagttaatt gtgaagattt tatagagttt tataatccgg gtaatatgag aataaataaa
     1081 gaagatttta ttcatggagg gcattcaaag gacagaaata gtatattatc gacgcttttc
     1141 agaagagtag gatattcaga aaaagctgga tctggaggac caaggatatt cgatgtagtt
     1201 aatagacata agcttaaaac gcctgaaata gaattaacgg acatggacac taatgtagta
     1261 ctttggaaac aagatttaat gaaggagttt gaaaaatatc ctgagttaga caaaaaagta
     1321 ataaagtata ttattgacta tggatcaata agtaagggtg aagccttaaa aatggaaaat
     1381 atgacagaat atcagtttag aaatatttta aaaaaactaa aagatgataa cttgataaaa
     1441 aaagaaggtg aaggtccggc tactaaatat gtgttaatag aatcaaaaga agctgatata
     1501 ttgcgaacta aaaaagtaat taaaagttta gagtctttct ttaggaataa ataaaaaaac
     1561 aagagatagg tgcgaagtgt ttggattata caaatgcttc gcatcttata tttattaata
     1621 aatatcatag aaaaccgtat cattaaccga tacgcagaga tgcggttttt tagacacttc
     1681 ataaagggat tttgaacgta tcagaacata tgaggtttat aggaattgct gttatgtttt
     1741 ttgatcacat caataaacaa aaaaggtatg tactatgtaa aatatttatt aaatgatata
     1801 aagcgagggt atataaatga tttttaaata gatatttatc caataatata aaaaggaact
     1861 ataagctata tctaaaagct atatctaact acttatagtc cttcttcatt agtataaata
     1921 taattattaa gaaaggtata tatctttgta accttcgttt acattaataa tgttgtgata
     1981 acctttgtgt tctaaaatac caatagctat agaacttcta atgccagact gacagtctac
     2041 ataaataaca tcgtttctat tgaaaggtaa atctgtttct aaaagtttgc cgtgtggcac
     2101 atgaaccgct tgggataagt ggccattatt ccattcatta tcattacgta catctaagac
     2161 atgtgcttca ttaccagtta tgtctttact atgaacagat tgtgtttgaa tttgaggttg
     2221 tggtaactga tatccagaca cattatcata tccaataagt tgtaaagtat gttatgtgtt
     2281 gcttttgaaa caaggtgata gtctccaatc aagttaattt cttgattata gtttagatac
     2341 cagccaattt gattgatgaa atttttatca tatggaatat tgattgtacc ttcaatatgt
     2401 ccaccatgat aagcctcctt actgcggaga tcaaaagtta atctgtttgt acttgtagct
     2461 ggataaaccg tataaggttg atataaattc ataccgaatt gattaatttt tttcatttgt
     2521 gcaaaatgat gtggtggtgc aggttggtca gaaatgagtt tatcgataaa ggtagcttca
     2581 ttattttcag aaaaagccca gttcgtttgt ttttcatagc caagcgtaga tgttggaata
     2641 gcacctaaag atttaccaca aggactacca gcgccatgac caggccaaat ttgaatgtag
     2701 tctggcaagt ctttaatact ttcaatagat ttaaacattt gttttgcgcc tatttcagat
     2761 aatccttcta ctttaacagc tttttctagt aaatcaggtc taccgatatc tcctacaaaa
     2821 ataaaatcac cactgaatag tcccattgga acttgtgctc cagcaccttc gtcagtaagt
     2881 aaaaaactta tactttctgg cgtgtgacca ggtgtatgaa gcacttttaa ttttatattt
     2941 cctacataaa tatcatcatt atgttgaaca aaatgagtgt ggttaggcat atttttataa
     3001 cctaacatgt catcactttc gcccgataca taaatactag catttaactt tatagcaaca
     3061 tctctaattc ctgaaacaaa atttgcatgt atatgtgttt cagctgcatg agtaatggtt
     3121 aaactctctt catcggcaac tcgaatatat gaagataagt cacaaatagg atcaatgatc
     3181 atggcttctc cagttttttg acaaccgatt aaataagatg cttgagataa atgtttatca
     3241 taaattgatg atagtcaatt tgttatcaag ggtgataatt atattaacta tggtaattac
     3301 atggaattaa agttattaaa caaacttgat gatgaccaat tagatttaga atttttacaa
     3361 gataatgggt taaaaggtaa tgttcaaggt cctatgactt taaaggaacc tttaaaaagt
     3421 tatatacaaa agtcttgaaa acgaatattt taaatttaga taatgagtta gaaaacactg
     3481 aatatgtcga aggtaaacca tatgtacagt acggtattaa atatgaaaga aatcaggcat
     3541 taagaaatga agctattaaa attcatggaa ctacatgtaa agtatgtgga tttgatttta
     3601 aagctaagta tggcgattta ggtgagggtt ttattgaaat tcatcattta aaaccaatgt
     3661 tttcaataaa aagagaaata aaagtaaatc cacaaaaaga tttagtccca ctatgttcta
     3721 attgccataa aatgattcat agaaatacta aaaaaccttt aacgattaaa gaattaacca
     3781 aaatagttaa ttataatagc aaataattta atattttata aactatcatt caaccctctt
     3841 aatttattag gaggtttttt gtatttatgc tttcaaatgt gtgatatact ttgtttgttg
     3901 aaatatagag tatctataga tagggtgatt gagtatgaaa ttcactgaag tggaagttat
     3961 cgaacatctt gtaaaggcat ataaagaagc aggaaagcct acttatcctc atgaaaattt
     4021 atatcgagga cgtaatcata gtatttcagg tattggagaa gacttgctgg gtgcttattt
     4081 gattagtaga ttggaaggtg tccaaatatt tattgatcag cctttatcta tgattgataa
     4141 atctttaagt acaagatatc cggatttatt aatttgtgaa gataatgaaa ttaaaaatat
     4201 actagaagtt aaaatggact taggatatca aagaaaagat tttatagatt attgccgaaa
     4261 gaaagaagaa tggatttcaa atatcgtagg aaaacagtgt gtattgtcta gaaagagaga
     4321 agacaaaatt cctatgaata tagctgatga tattaaattt catgttgtga tttacagtga
     4381 aaacaatgga ccgaagcggt ttgatgaaga aatcatgcct atcgttaatg aaacatgtcc
     4441 acatattgaa gtatatgtcc taacaagcgg tcaacaccct aatttagtaa atgttaatct
     4501 tgaaggtatt aatattaata aagatgaatt tgaaatatta gtaaatgcgt tataaaaaaa
     4561 tagagcatcc tccacgttat ggaggtgctc tgttttttat tgaaaagtat caagttaatt
     4621 aatttaatat gcttaataag ttctaccttg acctttttct ctagcttctg ttcgatctct
     4681 tatgtactca gtacatactg gattttctgt taattcatca actgtattag tttaatcaga
     4741 agacgtgtct actttgtaag cttctaagaa cttattatat gatatagcgt ttgagttttg
     4801 ttgttcttct ataaatttct gtagttattt ttcaaaaacc gcatcattaa ctgataagca
     4861 gaggcgtatc ataagtaaaa ctaaaaaatt ctgtatgagg agataataat ttggagggtg
     4921 ttaaatggtg gacattaaat ccacgttcat tcaatatata agatatatca cgataattgc
     4981 gcatataact taagtagtag ctaacagttg aaattaggcc ctatcaaatt ggtttatatc
     5041 taaaatgatt aatatagaat gcttcttttt gtccttatta aattataaaa gtaactttgc
     5101 aatagaaaca gttatttcat aatcaacagt cattgacgta gctaagtaat gataaataat
     5161 cataaataaa attacagata ttgacaaaaa atagtaaata taccaatgaa gtttcaaaag
     5221 aacaattcca agaaattgag aatgtaaata ataaggtcaa agaattttat taagatttga
     5281 aagagtat
//
LOCUS       DALAGF010000143         5268 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.144, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000143 DALAGF010000000
VERSION     DALAGF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.144"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            78..764
                     /locus_tag="O2692_002566"
     CDS             78..764
                     /locus_tag="O2692_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCZ4300164.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKELNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQ
                     AALDEANSLNKLSFKYIDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            754..1413
                     /gene="nth"
                     /locus_tag="O2692_002567"
     CDS             754..1413
                     /gene="nth"
                     /locus_tag="O2692_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCZ4300165.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            1418..1759
                     /locus_tag="O2692_002568"
     CDS             1418..1759
                     /locus_tag="O2692_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300166.1"
                     /translation="MTNITKEVFDNLEQEIDLFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDKLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(2325..4508)
                     /locus_tag="O2692_002569"
     CDS             complement(2325..4508)
                     /locus_tag="O2692_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCZ4300167.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(4505..5182)
                     /gene="recU"
                     /locus_tag="O2692_002570"
     CDS             complement(4505..5182)
                     /gene="recU"
                     /locus_tag="O2692_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCZ4300168.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNTFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
BASE COUNT         1776 a          832 c          886 g         1774 t
ORIGIN      
        1 ttaagcattt tagtcgaaaa ttaaagaaaa tgatttatac tattaagtaa tggttaaatt
       61 taaaaggggg cacgaacatg gataaatatc aattaaaagc aagacctgta gtgatacgaa
      121 gagaattatt agaccattac agtgacttag gtttagatga gcaagattta gtcattttgc
      181 ttaaattgat ttatgcttcc gaaacatcaa ataaacagcc ttcaattgaa ttgcttcaga
      241 agggctcaac tatgcaacca cgtgatatta caatggttat tcaaaatttg atacaacgtg
      301 aattgctaga attacaagtt caaaaagatg aagaaggtag atttaccgaa tatatgaatc
      361 tagatccttt tttcgaaaaa ttaagtcata tattgaagca acaaagtatg gaaacgaaag
      421 agctaaatag taaagaaaaa tttaaacaat tatttagagt tttagaggat acatttgcaa
      481 ggccgttatc tccatatgaa atagaaacgt taaatcaatg gattgatgtt gataaacacg
      541 atactgcaat tatacaagcg gcattagacg aggcgaatag tttgaataaa cttagcttta
      601 aatatataga tcgtatttta cttaattgga agaaaaacaa tgtcaaaaca attgacgatt
      661 ctagaaaaat aagagaaaaa ttcaataaac cgaaaatgac gcacacagtt aaaacggtac
      721 ctaaatttga ttggttaaac ggggagaacc ttgatggtaa gtaagaaaaa agcattagaa
      781 atgattgatg ttatagcaaa tatgtttccg gatgcagaat gtgaattaaa acatgataat
      841 ccgttcgaat taactattgc tgtattattg tcagcgcaat gtacagacgt tctagtaaat
      901 agagtaacaa ctgagctgtt taaaaaatac aaaacgcctg aagattattt ggcagtcagt
      961 gatgaagaac ttatgaatga tattcgttct atcggtctgt atcgtaacaa agctaagaat
     1021 attaagaaac tttgtcaatc tttgattgat caatttaatg gagaaatacc acaaacacat
     1081 aaggaattag aaagtttagc aggtgtagga cgtaaaactg ctaatgtagt catgagtgta
     1141 gcatttgatg aaccttcatt agctgttgat acgcatgtag aacgtgtttc taaacgctta
     1201 ggtattaatc gttggaaaga taatgtgaga caggtagaag accgtttatg ttctgtcata
     1261 cctagagata gatggaatag gagccatcat caactcattt tctttggaag ataccactgt
     1321 ttagcccgaa aacctaaatg cgatatttgt ccactattag aagattgtag agaaggacaa
     1381 aaacgttata aagctagttt gaaagaagcg tgataacatg acaaatatta caaaagaggt
     1441 atttgataat ttagaacaag agattgattt atttgccaaa aataagacat taggttctag
     1501 tgaagcaaag ccctacttgg atgaatacca tagtaaaatt attgattatt ttaagcaggt
     1561 taatgacata actggcaata ttgattttga taaattaaat caatatcctg ttgtgccaat
     1621 gaattttaaa gaaagatatg attatatgat tgaacgtaaa tatcatttta tgggctatcg
     1681 acaaatgaag acctttaaaa ctgaattgat taaaatgaat gctagttatc aaacaaggtt
     1741 aaagaataag caggtataga caagattgat catttcctaa tgttgaaaat atataaaact
     1801 aattcatgca cacatattta taattatgtt aggtgtcgtg tcattacaac gtcttaatat
     1861 aaaaactgtc catctacatc ataatggata tcttgagata tgctttaaga tatctatttc
     1921 gaatcgtagg tggatttctt atatctatga ttgtgaaaat atgttgcaaa catgttgata
     1981 tttcactgat taacgtacac agggaattaa taaatatatc aatttaaaac agcgtctaag
     2041 atgactatga gaaaatataa tcataatttt aaaaatggga acaaccactt attcaaattc
     2101 aataatttta catagctaaa gatgtgaata tgaaaaacta tttagatgtt tcaaaatgta
     2161 tgtgaatgac tgattttacg gtttctacta atatagcaat agcgtatgaa tacttataaa
     2221 aaaagcagga agttttacct tcccaccata aaagatgaag aacctaaaaa aatcctcggc
     2281 tacaccataa atatggtata actgaggatt ttatgttgag tggattagtt gaatatacct
     2341 gttaatccac cgctgtttct agattgttga gatctagtat tgttatttga ttgtgcagta
     2401 ccactgctgt ttgctgatgt gtcactacca ccgtgtgtac tacgatttgt agtgttgtta
     2461 tcttgactac cagaaacatt gattgatggg atactaccac ttactgagct aggacgttta
     2521 aagtcttcgc catctctaga tgaaattttt gacatcacat tttcatataa gaactgtgga
     2581 tattcttgtt ggctatgtcc cacaaatgag ttttcaccat attgtttaac tttactgaag
     2641 cccatccaca ctgacatagt gtattgaggt gtaaagccgt taatccacac gtctttcgct
     2701 gcattatcag gtaaattata ttgtgaataa gtttcagcac cgtaagtacc agtacctgtc
     2761 ttagcaccca tatttactcc agatacacca tggccatatg cagaaccata tggtttaaat
     2821 gtacctttta gcatctcagc taacatgtat gcagtgtaat cactcatcgc tttatggcta
     2881 gtatgatcgt attcgattgt ttcaccatca cgagtaacta ctttttgaat tgaatgcgcg
     2941 ttgttataag taccaccgtt agcgattgca gcaaatgctg atgctaattg tgttggtgag
     3001 aattctgaag cagaaccacc aagtacttca gatggaccaa tatcgccttc gtagtttaag
     3061 ccaagtttgg cagcgaattt cttaggtgca tcattaccag cattttgctt aactgattgc
     3121 caagctttta aagctgggat attgaaactt tgtcgtaaag catcataaat agatacagta
     3181 ccgtgactct tcgtatcata gtttctaaat gtagaaccat caacttgata tgaagattca
     3241 tcttgaatcg catggtttgt tgcccatttc atattttcaa tggcaggtcc atacgctaag
     3301 aaaggtttta aagatgaacc agtagggtga ggatctgttg cttggtttct gttaacgacg
     3361 tctttgaaat cacgtccacc agatatagca actaaaccac cagttttact atcaagaatt
     3421 gttgcaccaa cttgttggtc tttattcttg tagaagctac cattatcaac atcattttgt
     3481 aatgtttttt gaacatcttt atccatgttt gtataaattt taataccact ttgtaataca
     3541 ttacctaaat tttcatcttt gaatgcttta ttattcatta attcagattt tacaaagtta
     3601 acgtatgaat tatactcaga atcttgattt gtatcaatgt tttgacgttc ttcaggagta
     3661 cgatttacta agttcgcttt taaatcgatt ttcttagcat cttcccactg tttatctgta
     3721 atgcgtttat gataatgcat taagtataaa acagtgtttt tacgatcttc agcagctttt
     3781 ggatgatcat aaatattata gttgtttgga acctgaggta aaccggctaa ataagcttct
     3841 tccgctaagt ttaaatcttt taaatcttta ttaaagtaat acttagcagc agctttaata
     3901 cctgttacgc catcagaata gtaaattttg tttagatata cttggaagat atcatcttta
     3961 ctatactctt gttctaaacg atatgataag tatgcttctt gagctttacg tccaatagat
     4021 ttatgttgtg ataaaaatgc atctttaaca acttgttgtg ttaatgttga ggcaccttca
     4081 gaaccaaatc caccagtcaa gttcttacca attgcaccga ataaacgttt ataatcaagt
     4141 gcgccatgtt cgtagaaacg attgtcttca gttgcaagta ctgcgtcttt cattgatttc
     4201 ggcacgtctt ttaaatttac atgctcatgt ctttggccat tatctaatgt tttaacaagt
     4261 tctccgttct tgtcatatat ctttgcagga atcggatctt gtaatttagc ttcggtaaaa
     4321 gcaggtgctt tccaagcata ataagcaaac aataagatac ctagtaaaag aacaacgaaa
     4381 aatgcaataa tcatgaagcc aataatctta ataatcgttc tcttcacatt tctattcttt
     4441 tttgagttgg atttcccacc attattaccg tttttcttag gctgagaaga tcctttgttt
     4501 tccgtcatac gcggtcctca ctttcatcta atatcaactt atcaactgct tttagatagt
     4561 ctaatcttgg ttgatactga taaggaatat ggtaaccatt ttttcgtatt tcatcaactg
     4621 ttatagactt tttaatatta tctttatatc tcttccaaaa tacttcgaat tttgaatagg
     4681 gtaaaagata aacttcatct agcgttttaa aacgaatcat taaaaacaca ataccttttt
     4741 gttgatatgc atttttcata tgttcgactt gatggtcatg aatattattt aaaggaaagg
     4801 acgtcttgtt tttagtttcc tttgcttcaa aatcaatata ataaccttga taaacgccgt
     4861 tgtaatcagt tgttgaaggt gtacgaaaat aagcttcgtt aatcacagct ttactccgct
     4921 taggatagtt gacattaact atttgtactg gcgtaggctt tttgtgaata actgcaatgt
     4981 cgcttttaag ataaaacgta tttgaatgtt cgatatcttt ttcaagtgac ataccacgtc
     5041 caccatactc aatattacta aaggcagcgg tctttttccc tccgtctata gcactatttt
     5101 tacgatatgg tttaccattt ggataattca taaatttcac cacacaagtt tggtagtaaa
     5161 cgttgtttat tatacacaac attatagtga aacaccaaac ttaaattagt ataaatgcga
     5221 aagacacaat acaccaaagc ttattttaac gtgatttcag cattaaca
//
LOCUS       DALAGF010000144         4907 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.145, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000144 DALAGF010000000
VERSION     DALAGF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.145"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(219..740)
                     /locus_tag="O2692_002571"
     CDS             complement(219..740)
                     /locus_tag="O2692_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HCZ4300169.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(768..2063)
                     /locus_tag="O2692_002572"
     CDS             complement(768..2063)
                     /locus_tag="O2692_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCZ4300170.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVAPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(2103..2579)
                     /locus_tag="O2692_002573"
     CDS             complement(2103..2579)
                     /locus_tag="O2692_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCZ4300171.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(2748..3284)
                     /locus_tag="O2692_002574"
     CDS             complement(2748..3284)
                     /locus_tag="O2692_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCZ4300172.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATKWNSILQ"
     gene            complement(3495..4433)
                     /locus_tag="O2692_002575"
     CDS             complement(3495..4433)
                     /locus_tag="O2692_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCZ4300173.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDNNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED"
     gene            complement(4703..>4907)
                     /locus_tag="O2692_002576"
     CDS             complement(4703..>4907)
                     /locus_tag="O2692_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ4300174.1"
                     /translation="TLKNFCLTLFRYAFFILWTVIFSYALLSMSYQNIIVYFVWIAVI
                     MGFFLVNIALIIDKNIHVILKNQ"
BASE COUNT         1588 a          910 c          649 g         1760 t
ORIGIN      
        1 ttattataat gtgaattaat tcacaaagta tatagctatg atcaaaaaaa tgtgtcagtt
       61 attcaaattt tttattaact acagattaat ttaatttcaa aaaaattaaa aaaactccca
      121 atcaatctaa gacattattt tcacctcacg atttacgtcg tcattgagaa atgaatcata
      181 tctttataga ttaattggga aattttagtt tctaatattt aattaaatgc ttgatgtaaa
      241 gcatcagaag aatgcttcca cataccttca tcatggttca ttaaaaagtc ttttaaaata
      301 gctttattct cgtcaccaat ttgatctatg ataatatgat gtttcattga tttatccatc
      361 atattaacat gttcaggcat acttttataa ccacgacgaa ttgctttatt cactaataaa
      421 tagcacgctg ttaaaccagc gtaatatggt ccttgttcat tacgttctgt tgttacccaa
      481 cacaaccaat attctttagc ttcatcgcct tcaacagcag ctttatcagt aatccatttc
      541 acaccttttt caactttggc acgagcatgc atgcctccaa tatcaataaa tgcttctttg
      601 ttagcaacat cgataaaaac tggtgcaata ttgtctaagc taattgaacc aatatttgta
      661 cctttatgac catctagagg gtcatttttt atgatattaa aattaaagcc ttttttctca
      721 gacaatgata taacctccat atttaacgat aacaatttta gccctgttta attaagctca
      781 ttaatcaaat tttcaatagc ttctctaatt gtcttatctt taatctttgc aaccatttct
      841 ctaggataat ataatttata gtcttggcga ttaatgcctg taatactatg aatgactaat
      901 gattgattgc ttatttctct aatcgtacca tcttgtctta ataaatgaat cggtttgcga
      961 tttgaccccg gtcgatcata gtcatatggc aaatcagaaa atgcttcact cacaaaataa
     1021 taatctggat taataccacc tgcttcaaac agttcttgca gttctgatat tgtaataatt
     1081 gagccatcaa atggaatata tttaaataag tctcgattaa taaatcgact tgctaaatca
     1141 cttaaaatag catcatcttc ttttgtccat ttttccaaat aatacgtaac taccgcttca
     1201 tcgagttcaa catattgttc aatcgtaact gtctcttcaa aaaatggaat aaaatcatgt
     1261 ggatgcaact taaattcata gccttcatta taaagctgtt ttgcgcgttt caaacaatta
     1321 ttaagcagca cttctccacc acgactaact gggtggaagt aaatttgcca atacatttga
     1381 taacgactca taataaagtt ttcaactgca tgcataccac tttctttgat tagtacttca
     1441 tctttagaag gtcgcattaa tcttaaaata cgctccatat caaaagcacc atatgataca
     1501 cctgtgaaat acgcatcacg ttgtaaataa tccattctat ccgcatcgat ttgtgacgaa
     1561 atcatcgaaa tgaccaattt attatgatgc gttttattaa ttacttccgc aacttctctt
     1621 ggaaactcag gcgccacttt acgtaataca gcattcacct cagtatctcc agtaataatc
     1681 gcttgtgtgt atgcttcatg gtctgtatta aatatttttt caaaactatg tgaaaatgga
     1741 ccatgtccta aatcatgtaa taatgcagca cataatgcca acggtctatc tttattgtcc
     1801 catgcatcat gaccaataaa tgactcatca attaatcgtc taactatttc atacacacct
     1861 aaagaatgtc caaagcgact atgttctgct gtgtgaaaag ataggtacag tgttcctagt
     1921 tgtctaattc gacgtaacct ttggaattcc ttagttttaa ttaaatccca tatcaattga
     1981 tcttcaacat gaatatatcg atgtatcggg tctttaaaaa ctttttcttc tactaatttt
     2041 aaatctacat atgcgttagt cattattccc ctccttttcg tttaatataa tatttaattt
     2101 acttaaaatg ctttgtacat aagtgctaag tctaactttt cgccatacat ttctggctca
     2161 taagagcgta agattgtaaa accttgctct ttatagtaag ctactgcttc ttcattttta
     2221 ttatctactt ctaagtaaac accttcaaat ttatcttcaa aacgtgataa tccttcattt
     2281 aacaatgctg taccataacc tgtatgttgc gattctggtt taacataatg agctgataaa
     2341 tataattctt caccgtaaat aaagttagca aagccaacga tgtcattacc ttcttcaacg
     2401 actaagaata attgttcttg aagtcttttc tttaaatgat gttcattata tgaagcttct
     2461 aacaagtgat taactgttgt cgcagcgtat atatttaagt atgtattaaa ccaagcttta
     2521 gttgcgacat ctctaatttg aacaacatct ttttcagttg cttgtcttac cttgaacatg
     2581 actttctccc cttattaaca agttttaata acggcattat accacaactt gctcaatact
     2641 taataaacaa tgattgtcta ttcaatttat atatttatat tttccgttaa aattaaaaat
     2701 aaaaaataac gaagcaaaaa atcacttcgt ttagtatgag gtatgtctta ttgcaatata
     2761 ctattccact tagttgcacg tgctaaggca tagttgtctt tcatgatgtc accaggcttt
     2821 tcagcagttc caataatata accatttaaa gtggcaccta aaaagtctaa actatatttc
     2881 atttgcgtaa ttgctggctt cgcttttatt tttggacaat ctccaccaac taaaataact
     2941 ctaaaatcct tttcggccat ttgtgcctta aaattaggat atcgtttatc ttgtaatgtt
     3001 tctgaccaat gttcgataaa tgctttcaat ggtgctgaaa tgctatacca atacactggt
     3061 gatgcaaaaa taattgtatc actagccaat attttatcta gaatcggcaa atagtcatcg
     3121 tcatatgaag taatagtctc tgctgtatgt ctcacgtcac gtatcggttt aaactgatgt
     3181 tgtgtcacgt caatccattg atactctaaa tcttgcaaag cgaattttgt taattgtgca
     3241 gtattaccgt ttggtctact cccaccaaac aaaacagtaa tcattttagc ctaacctcac
     3301 ttttgattaa taaatatctg tgtttttcgt tacctaatta tactatcata agctttgcct
     3361 accgaatagt aaaacgctta caacttttat ataaatttga cgaaatttcg tcatgcctta
     3421 tataacgtcg tttgtgatac ggggctaatt catgatgaaa ttagatacat atatcaccat
     3481 taaatacaat tcatttagtc ttcaatcgga aacagttcat cgatatattg aatctcatca
     3541 tctgataaaa cgatatctgc agctttaata ttttcaacga cttgttctgc acgttttgca
     3601 ccaggaataa tcacatcgat agctggtctc gttaaataaa atgctaatac aatgttcgca
     3661 attgaagttt gatgtgctgc agctatgctt tccaaagctt ttacgcgacg cacattttct
     3721 tcaaatacac ctggtttaaa atcacgacgt gtagtacgat ggtcactaaa tttagtgttc
     3781 tcatcatatt ttccagctaa aataccggat gctaatggga aatatggaat aaatgtgatt
     3841 tggtgatcaa cacaatattg taatactgcc tcattttcgc gatgcaataa attatattct
     3901 aactgtacaa catcaacgta accatcttta tttgcttctt taagttgatc taatgtgaaa
     3961 tttgatacac caattgcttt aatcttccct tgttccttaa gctcttgtaa tgctgcaact
     4021 gcttgatctt tcggagtgtt gttatccgga aaatgaatat aatataaatc gatataatca
     4081 gtttgtagac gtttcaaact attctcaact tgttgtttta aatattccgg ttgattgttc
     4141 tgatgtactt cttgattttc atcaaattca tgagaccctt tcgtagcaat tttaatttgc
     4201 tctcgcggat attctttaac aacttctcca accaattctt ctgatcgttc tggcccataa
     4261 atatatgccg tatctaataa attaatacca tgattaatgg cttgacgaac aacatctttt
     4321 ccttgttctt catctaagtt cggatataaa ttatgcccac ctactgcgtt cgtcccaagt
     4381 gcgattggaa acacttcaac atcagattta cctaagttta caaattgctt cattagaccc
     4441 agctccttat ctttataact tttccactat cacactatca tgatttaata caacttccaa
     4501 atgttagaat tagagcttga atttaagcgg tgtaatagga aaacgttata taaaactcag
     4561 ttaaaataag tattgcttaa attgatatgc atatagcgcg attgctcgtt gacctttaaa
     4621 ttacatgtac ttttgaatca gaaattgaaa aacatttaag aacgacaata ttaaagccat
     4681 tgcaaattta atttggtaat ttctattggt ttttaagtat gacatgaatg tttttatcta
     4741 taattaaagc tatattaact agaaaaaaac ccattataac tgcaatccaa acaaaatata
     4801 ctattatgtt ttgataactc attgataata gagcatatga gaaaatcact gtccataaaa
     4861 taaaaaaagc atatctaaac aatgttaaac aaaaattttt tagtgtt
//
LOCUS       DALAGF010000145         4845 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.146, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000145 DALAGF010000000
VERSION     DALAGF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.146"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(239..985)
                     /locus_tag="O2692_002577"
     CDS             complement(239..985)
                     /locus_tag="O2692_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HCZ4300175.1"
                     /translation="MPIEYFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVESHS"
     gene            complement(990..1463)
                     /gene="ytkD"
                     /locus_tag="O2692_002578"
     CDS             complement(990..1463)
                     /gene="ytkD"
                     /locus_tag="O2692_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HCZ4300176.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIYYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            1529..1786
                     /gene="yidD"
                     /locus_tag="O2692_002579"
     CDS             1529..1786
                     /gene="yidD"
                     /locus_tag="O2692_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCZ4300177.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(1783..2784)
                     /gene="menC"
                     /locus_tag="O2692_002580"
     CDS             complement(1783..2784)
                     /gene="menC"
                     /locus_tag="O2692_002580"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCZ4300178.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVADGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGAKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(2789..4267)
                     /gene="menE"
                     /locus_tag="O2692_002581"
     CDS             complement(2789..4267)
                     /gene="menE"
                     /locus_tag="O2692_002581"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCZ4300179.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMKSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGRDITTNSLLDNTMGIQYETSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNVYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(4426..>4845)
                     /locus_tag="O2692_002582"
     CDS             complement(4426..>4845)
                     /locus_tag="O2692_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HCZ4300180.1"
                     /translation="LSSCSTNNKIVGQRFEIQQHNETIGSIYFSADYAHIRGIEKGTA
                     KYFIDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYLIENLDDEVFHLS
                     ALQDMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
BASE COUNT         1435 a          991 c          746 g         1673 t
ORIGIN      
        1 taaagagcca accgttgtta tagtctaaca atggttggct cctcttattt tatgtgctaa
       61 aaatttatag gcaattttat tacaacaatg tacatttaag gtgaccttca tgccaaaatc
      121 gcatcactca tttaatggaa gcagcacgtc ttcatataaa gtaccgatcc ctaattcaac
      181 gcatgtagta ccacatcttc aaagcttgat agttcccatg cacacaccac gtttcatact
      241 agctatgcga ctcaacttgg ttcataaact ctttaatata agtcaatgtt tcaaccatcg
      301 ctggtggtct tggcacatgt ccttctgcca tttgataaaa tgtttcatgc gtggcacctt
      361 ttaactctag ttggtccgct aaataatacg catgatgaat accaacttgc tggtctttcc
      421 ctccatgtac aattaatatt ggcggactgt tttcattaat gtttggaatc gcttggcgtg
      481 cctcatatgc cgctcgatct tttttcggat gaccaatcat tcttcgtagc atgcctctta
      541 aatcgacacg ttcttcatac attaaatcaa tatctgagac accaccccag attgtataac
      601 ttgttactgg taagtcttga aatgtcaaca atccttgtaa accacctcgc gaaaaaccaa
      661 ccatgtggat aaatgcatgt ggatatttat catgtagcaa ccttaataat tgcgtcacat
      721 catttaaatc gccacggtaa aattcgtctt tgccttcact cccattgtta cctcggtagt
      781 atggcccaat cactaaagtt tgactatctg aaaattgcat taatctacct gcgcgcacac
      841 gtcctacttg acctttgcca cctcgcaaat aaactacaat gcgatttact tcatgatgtg
      901 gtgtcatcat taaagctttt acttgtaagt catctgacaa atatgtaatt tcttcgaatt
      961 gatgcgtaaa atattcaatt ggcattcgtt tacgtttgat aaaacccaag tgattgcacc
     1021 ctctctacgc attttaaaat ggtactatct tgcagtaaga aactccgttg tgcgagttca
     1081 atatcattga tacagttaaa caacactggc cctgctgttt ctaaataatc gttcttgctt
     1141 accaatgatt caacttcgat aaaatataca tcttttacaa aatcagtttg atcatgtgtt
     1201 tcaatggtat attgtgctat gtaataaata tttttaactt tggcgcctgt ttcttcatat
     1261 aattcacgtg taactgcttc agcactactt tccccgcgtt cccttttacc accaggaaat
     1321 tcaatccccc gtaaattatg tttggtaaaa agcaattgat ttttaaacgt tggaatagct
     1381 agcacatgat tgccatctgc tatctcatta tcctttttaa atgtcaaatt aacttgacga
     1441 ttatctttat ccctaaactt cacgcgcatc acatccctac attgtatgtt aatataatag
     1501 ttaattacta tcgttggagg cattaattat gaaaaagata ttcttggcga tgattcattt
     1561 ttatcaacgt ttcatttcgc cactcactcc accaacttgt cgtttttatc caacatgttc
     1621 agagtacact agagaagcga ttcaatacca cggtgctttc aaaggccttt atttaggtat
     1681 ccgtcgtatt ttaaaatgtc atccgcttca taaaggcggc tttgaccctg ttccgttaaa
     1741 aaaagacaag tcagcaagca agcattcaca taaacataac cattaatatg gttgtaattg
     1801 agttatatcc actaaagggg ggcgaaattc gagtcgcccc tcttttaata tgcctgaatg
     1861 cgccaccaca tcttgttcaa aataataacc tgctggtgta acatctcctg gataatcacc
     1921 tttacgagca agcatcgctg taaaatagcg gcttaaacca tattcgtaca tgccgccaat
     1981 aaccactttt gcaccatgac ttttcaaagt atcaattgcc gtttgcactt tatcaatgcc
     2041 acctagacga aatggtttta atacaacaac tttcacattg tataattcta tcaaattaat
     2101 tatgtccaac aacgatgttg ccttttcatc aagggctatt ggaggtattg ttccatccgc
     2161 tacttcatca agcatggaga tatctttaaa tggctcttcg atataaagaa cctgttcacg
     2221 cgctaataac tgtaactgtg tgaaatcttg acgatccaag gactcatttg catctataac
     2281 caattgaaag tgaaagtcta attcccgtaa cactctaatt tgatgcatga tttgaggcgt
     2341 ccattttaat ttaattctgg tcggctttgt tgcttttaat gactctagtt gtttatttga
     2401 taagccgctc gctgtcgctc catatgctac tgaaaatgaa ggcagtacat gaaacatttg
     2461 atacaatgcc atgacaatag ttgcccttgc agcaggcgta ttttccaatg aatctactaa
     2521 ttttagtgct gcttcatacg tttcaaatga tttatttcta ttatcttcga accattgctc
     2581 aattacatgt ttcactgagg caattgtttc atgatcatac caatctgttt gaaaagcgtt
     2641 acattccccg aaatatgcat ttcctttgtc atcaatcaat tcgataaaca aacaatcacg
     2701 atgcgttaaa gtgactttcg gtgttacaat ttgtgactta aatggctcac tatatttata
     2761 aaaatgcaaa gctgtcaact tcatcaaatc atcctctata caacttattt ctttgtaatt
     2821 tacctgttga tgtataaggt aaagtatcaa ccttttcaaa gtgtttcggt actttatatt
     2881 tcgctaaatg ttgtgataaa tatgcaatca attgtgcctt tgaaatgtca ctttcactga
     2941 caaaatataa tttaggcact tggccccaag tatcatcagg atgccctaca catactgcgt
     3001 cactgatacc tggaaattgc ttcgctaccg tttcaatttg atatggataa atattttcac
     3061 cgccactaat aattaaatct ttacgtcggt cataaatcat gacataacct tcatgatcta
     3121 tttcagcaat gtcacccgta ttaaaataac cattttcaaa cgtacccgtt aaatctgttg
     3181 gatacaaata tacattcatc acattggcgc ctttaatcat taattctcca tgaccttctt
     3241 tattaggatt tttaattttt acgtcaacat tggcacttgg catccctaca gtgtcaggac
     3301 gtgcatgcaa catttccggt gttgctgtta aaaattgcga acatgtctca gtcataccaa
     3361 atgaattata aattggcagg ttatattgta atgccgtctc tatcatagtg gcagataatt
     3421 tagcaccgcc gagtaatatt ttttgtaaat tataaggttc atgtaaacct tgttgcataa
     3481 gccaatttaa agtttgtggc acaagcgaaa tgtgcgtgat tcgttcattt ttaatcatcg
     3541 ttaaaatttg ttcggcattg aatttatcaa caatgcgcac agtaaaacct tcaataacag
     3601 ctcttaaaag tacactgaga cccgaaatat gataaatcgg caagacagat agccaattag
     3661 tgtcacgatc aaatcccaag ctctctttac atccgattgc actggcataa tgattacgaa
     3721 acgtttgtgg caccgctttt tgagggcccg ttgtccctga tgtaaacata atcgatgcaa
     3781 tgtcatctaa attaaatgat gtatttaata tgttggacgg cgactctttc ggcaccacag
     3841 tttcattcga tgtttcatat tggataccca ttgtgttgtc caacaaactg ttcgttgtaa
     3901 tatcccttcc agcgaattca atatcatcca gcgatacaat ttgaaaccct cgtaattcca
     3961 gtggcaaggt acaaaaaatc aattgtacat cgattgactt catctgattc gtcatctcat
     4021 taggtgtcaa ccttgtatta atcatcgcaa tttcaatatt tgccaaccaa catgcatgta
     4081 ttaaaatgat cgattgaatc gaattatcta tgtatagccc aacacgagat tgttgataag
     4141 ccttgagtct tttagccaat agactcgctt cacagtataa attttgataa gtataagatt
     4201 cttgaccgtc tgttatcgca atatgatgtc cattttgttg tgcttgttta tataaccaaa
     4261 agtccatgcg ttattcctcc aaaatcattt acattataat tataacgatt ttatgacatt
     4321 ctagcagtgg ttatgtttaa aaatataaaa aagtagacga attgatgcat tgatatgatt
     4381 gttataatgc tcaatacata tcgttatatc attcgtctac tattatcagt tatttttatt
     4441 taattttagt gtcattctgt cattttgatg tggtgattta cccattgttg ccacatcatc
     4501 tgcaatgtca attggtatac ggttcatgtc ttgtaatgca cttaaatgga atacttcatc
     4561 atctaaattt tcaatgagat atacataata tgttaccttg tcctttttat attttaacgt
     4621 tttccaaaag tccggcttgc aattcaatac attatccgga atatattcaa taaataagta
     4681 acgtttgctg cctactttgt ctatgaaata ttttgcagtg cctttttcta tacctcttat
     4741 atgtgcatag tctgctgaaa agtaaatact acctattgtt tcattatgtt gttgtatttc
     4801 aaatcgttgg cctactattt tattatttgt gctacaacta cttaa
//
LOCUS       DALAGF010000146         4793 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.147, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000146 DALAGF010000000
VERSION     DALAGF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.147"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..252)
                     /gene="lpl1"
                     /locus_tag="O2692_002583"
     CDS             complement(<1..252)
                     /gene="lpl1"
                     /locus_tag="O2692_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl1"
                     /protein_id="HCZ4300181.1"
                     /translation="MGYLKRFALYISVMILIFAIAGCGKGNETKEDSKEEQIKKSFAI
                     TLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWTIYTDFAK"
     gene            620..928
                     /gene="spn"
                     /locus_tag="O2692_002584"
     CDS             620..928
                     /gene="spn"
                     /locus_tag="O2692_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HCZ4300182.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
     gene            complement(1031..2539)
                     /locus_tag="O2692_002585"
     CDS             complement(1031..2539)
                     /locus_tag="O2692_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HCZ4300183.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASENQNALISNIN
                     VDNQEKQNNVNQAVQPQNNTNETSKVPANFVKLNDIKPGDTSIQGTTLPNQFILLTID
                     KKDVSSVEDSDSSFVMSDKDGNFKYDLNGRKIVHNQEIEVSSSDPYLGDDEEDEEVEE
                     TSTEEVGAEEESTEAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKG
                     HTSVKGKVALSINNKFINFETNANGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRF
                     DDLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLK
                     DVKEDLHVDEIYGSLYHTEKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEGQEFPD
                     LQVDEKGEFSFDVDHAGFRLQNGETLNFTVVDPITGELLSGNFVSKNIDIYESPEEKA
                     DREFDERMENTPAYHKLHGDKIVGYDTNGFPITWFYPLGEKKVERKAPKLEK"
     gene            complement(2561..3238)
                     /locus_tag="O2692_002586"
     CDS             complement(2561..3238)
                     /locus_tag="O2692_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
                     /protein_id="HCZ4300184.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKASTLEVRSQATQDLS
                     EYYNRPFFEYTNQSGYKEEGKVTFTPNYQLIDVTLTGNEKQNFGEDISNVDIFVVREN
                     SDRSGNTASIGGITKTNGSNYIDKVKDVNLIITKNIDSVTSTSTSSTYTINKEEISLK
                     ELDFKLRKHLIDKHNLYKTEPKDSKIRITMKDGGFYTFELNKKLQTHRMGDVIDGRNI
                     EKIEVNL"
     gene            complement(3621..>4793)
                     /locus_tag="O2692_002587"
     CDS             complement(3621..>4793)
                     /locus_tag="O2692_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCZ4300185.1"
                     /translation="FPGFEGEWEEKQLGDLTDRVIRKNKNLESKKPLTISGQLGLIDQ
                     TEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRYDSGVLSSLYICFS
                     IKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFFTILIKYPSLEEQQ
                     KIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEDYPDWENSKIE
                     KYLKERNERSDKGQMLSVTINSGIIKFSELDRKDNSSKDKSNYKVVRKNDIAYNSMRM
                     WQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKFKINSQGLTSDTWN
                     LKYKQLKNINIDIPVLEEQEKIGDFFKKMDILISKQKMKIEILEKEKQSFLQKMFL"
BASE COUNT         1435 a          848 c          567 g         1943 t
ORIGIN      
        1 tttggcgaaa tctgtatata tcgtccacat ccctttgtca ccttttttaa attcgctatc
       61 tcgatatcct tctttgtcat ataagtcctc gagattctta attggataca tatctaatgt
      121 tatcgcaaag ctctttttga tttgttcttc ctttgaatct tcttttgttt cattaccttt
      181 gccacaacct gctatcgcaa atattaaaat cataacgctt atgtacaatg caaacctttt
      241 taaatatccc atcgcttcac gtctctctgt tttatacttc atttatattc tcttttcata
      301 taaataatta attattttgt atatttctct tttacgttac caaaatatac atatacaatc
      361 aatttaattt cttaattaaa tttgtactta ttattatatt gcgtcatttt tttattattg
      421 tttcatttga tttaaaccct ttttgaaatc aaggttttat taggaaaaat cctcaagcat
      481 cgtattaaag attaattgca tttaaacaat atttgtcact ttaactattt attaaaaatt
      541 attaaatcga cttaataaac gtaattttct taatattcag tgttatgatg gagttacaat
      601 ttttagagga gtgatagata tgaaatttaa aaaggtttta gtagctacgg caatggtagg
      661 tgttttagca actggcgttg ttggatatgg taatcaagca gatgcgaaag tttattctca
      721 aaatggactc gtactacatg atgatgcaaa cttcttagaa catgaattaa gttatattga
      781 tgtgttatta gataagaatg ctgaccaagc tacaaaagac aacttaagat catacttcgc
      841 cgataaagga ctacattcaa tcaaagacat tatcaacaaa gctaaacaag acggctttga
      901 tgtttcaaaa tatgaacacg taaaataaat tcaataatgc tttgtaacaa agcacaaaaa
      961 acagctcaat gacgtagaag tcagtgatta actcactaat ttcattacat cattaagctg
     1021 ttttatttaa ttatttttct aattttggtg ccttacgttc aactttcttt tcacctaatg
     1081 gataaaacca ggtaatcggg aatccgttag tatcgtagcc gacaatttta tcaccatgta
     1141 atttatgata tgcaggtgtg ttttccattc tttcatcaaa ctcacgatct gctttttctt
     1201 caggagattc ataaatatct atgtttttag aaacaaaatt tccacttaat aattcacctg
     1261 taataggatc aactactgtg aagtttagtg tttctccatt ttgtaatcta aatccagcat
     1321 gatctacatc aaagctgaat tcaccttttt catcgacttg caaatcaggg aattcttgac
     1381 cttcacctag ttcagagtct accttcacaa ctgcatttgc gaatttagtc ttacctttaa
     1441 ttactttagt accttcttta tcaagaatac ctttaccttt ttctgtatga tataagcttc
     1501 cgtaaatttc atctacatgt aaatcttctt taacatcttt caatactttt actttttcca
     1561 ctttagtatg gtcatattta gtttctgctt tatcaatatc ttctaaactc gttaccttag
     1621 ttttgaaaat taatgacttc aatgcctcat cttcgtcatc aggtgcaaat gttaatgaga
     1681 tctcatcatt tttctttaac tctaaatcat cgaaacgttt cgttttgaag tcaaaattaa
     1741 agtatccttt gtcatcaata ggcatccaga ttccttctga tccagatttc gcttcttctt
     1801 tatttggacc accattagca tttgtctcaa agttaataaa tttattatta atagatagag
     1861 caactttacc ttttacagag gtatggcctt taataatacc tgaaccttca gtaataggtt
     1921 cgataaaaac ttggtgatgt ccatcttttt cttttagctg ttctttcggt atttcatacg
     1981 ctttttcata tcgcggtgtt gtatatgtag ctttagcttc tgtactttct tcctcagcac
     2041 caacttcttc agttgaagtt tcttctactt cttcatcttc ttcatcgtca cctaaatagg
     2101 gatctgatga agacacttca atttcttgat tatgaacaat tttgcgacca tttaagtcat
     2161 acttaaaatt cccatcttta tcagacataa caaagctgct gtcagaatct tcaactgagc
     2221 tcacatcttt tttatcaata gttaatagta taaattgatt tggtaaagtt gttccttgta
     2281 tagaagtatc acctggttta atatcattca atttgacaaa attagccggt acttttgatg
     2341 tttcattagt attattttga ggctgaacag cttgatttac attattctgt ttttcctgat
     2401 tgtctacatt tatattagag attaaagcgt tttgattttc agatgcttct gcttgtccgt
     2461 ttgtcataat atataacatt gtaattgcaa cagatactaa accgactttc attttacgta
     2521 acttaaaatt ttccctcatg atatgctccc tcgaataatt ttataaattc acttcaattt
     2581 tttctatatt tctgccatca ataacatcac ccatacggtg tgtttgtaac tttttattca
     2641 attcaaatgt gtagaaccca ccatctttca tagtaattcg aattttactg tctttaggtt
     2701 ctgtcttata aaggttatgt ttatcaatta aatgctttct taatttaaaa tcaagttctt
     2761 ttaatgaaat ttcttcttta ttaattgtat atgtagatga tgttgacgtt gatgtaacac
     2821 tatcgatgtt tttagtaatt attaaattta catcttttac tttatcaata taatttgaac
     2881 cgtttgtttt agtaatacca ccaattgaag ctgtattacc agatctatca gaattttctc
     2941 ttacaacaaa tatatctaca ttagaaatat cttcaccaaa attttgcttt tcattcccag
     3001 ttaaagttac atctataagt tgataattag gagtaaacgt cacttttcct tcctctttat
     3061 atcctgactg atttgtatac tcaaagaacg gtctattata atattcactc aagtcttgag
     3121 tagcttgtga tctaacctct aatgtacttg cttttactgg ctgagcagta gttgtaatca
     3181 tccctgttgt taaaatccct agtgctaaac ttgctttagc aatatttttt aatttcataa
     3241 ttctatgctc ccaattttta gtctatttga tttattctat tacgcaatac gaacaatcct
     3301 cattcattat aatgagcaat ggtcgtgctt cacattaaac ttactttaac taaaaattaa
     3361 tcattattaa gtaatttaat aaatagttaa ctaatatcat tcaattccta tcgatctact
     3421 ctcttttatt tacgaataac actttatctc aacttaatct ttatttaact acttattctg
     3481 tacacaattt cgacacaaaa aagacactgc gctttcacag tgcctaaatt taatgttcgt
     3541 ttttaattca ctttactttt acaactttta tgttaaatta tacaaatttt attcttatgc
     3601 aatctatgta tttatcaaag ttataagaac attttttgta aaaaggattg tttctctttt
     3661 tctaatattt caattttcat tttctgttta cttatcaata tatccatttt tttaaagaaa
     3721 tcacctatct tttcttgttc ctccaataca ggtatatcta tatttatatt ttttaattgt
     3781 ttatatttta agttccatgt atctgatgtt aatccttgtg aattaatttt aaatttatga
     3841 atcattctat gtgttttaaa cttatatcca ataaataatg agctagtatt ttgtgttgga
     3901 taaagcacag tatatgcagg gctaacaatc ccattataat ttgatttacc actagcccct
     3961 tgccacattc tcatagaatt atatgcaata tcatttttcc taactacttt ataattactt
     4021 ttatctttac ttgaattatc ttttctatcc aattcactaa attttataat gccactattt
     4081 atagttactg aaagcatttg ccctttgtca gaacgttcgt ttctctcttt taaatatttt
     4141 tctattttgc tattttccca atctggataa tcttcaccat tctcatcttt gaatcgcagt
     4201 tcctgtgaga aaattttctg catatagcct tttttctgtt gttgaagtaa ttcaagcttt
     4261 tgttcttcta attcaatttg tcggtcgagt ttgctgaaga acttgcctat tttttgctgt
     4321 tcttctaaac ttggatattt aattagaata gtaaaaaaat cattcacaga aacatttaat
     4381 aatccgtgat ttcttgcacc ctcaactgca attccagaaa cttctctata ccagtgtgtc
     4441 gaatcaaaat atgcttccat gaagtcttta gacatttcac ttttaataga aaaacaaata
     4501 tacaaagagg acaatacacc actatcatat ctagttaatc ttttaatagc ccctaatggg
     4561 tatccattag aataactttt gttatacgcg aattctccat tctttattag tgtataattt
     4621 tctagatttt tcgacgaaac tgatttacta aaatattctg tttgatcaat taaacctaac
     4681 tgtccggata ttgttaaagg ctttttcgat tctaagtttt tatttttcct aattactcta
     4741 tctgtaagat cccctaactg cttctcttcc cattcgcctt caaaccctgg gaa
//
LOCUS       DALAGF010000147         4615 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.148, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000147 DALAGF010000000
VERSION     DALAGF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.148"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(359..1003)
                     /locus_tag="O2692_002588"
     CDS             complement(359..1003)
                     /locus_tag="O2692_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCZ4300186.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLNIIKDILYLANMFRR"
     gene            1364..2161
                     /locus_tag="O2692_002589"
     CDS             1364..2161
                     /locus_tag="O2692_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCZ4300187.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
     gene            complement(2749..3756)
                     /locus_tag="O2692_002590"
     CDS             complement(2749..3756)
                     /locus_tag="O2692_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HCZ4300188.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
     gene            complement(3772..4389)
                     /locus_tag="O2692_002591"
     CDS             complement(3772..4389)
                     /locus_tag="O2692_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HCZ4300189.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
BASE COUNT         1652 a          776 c          627 g         1560 t
ORIGIN      
        1 ttgagatgag ctcaaactaa cattaaattg tttatattat ctatacttct tcctattaat
       61 gtttcgattt taaaattaat acgtagctat actttacttt tctaataaaa gtgtaattag
      121 aaaggcaagt acacctgttt aaagtttatc ttctaagatt tgtttacttt agaactatag
      181 cagttaatca tttcaatgat taataatatc tttacaatta aggtaatata tttttattgc
      241 tttatcagta aaatctcagt ctaagtttct aacttatgac aatcgctcca gtttaaggtt
      301 gatattcatt attgattatt ccaaaacttt atacaaattt ctttattaat ttaactgttt
      361 atcttctgaa catattagca aggtaaagta tatccttaat aatattcaac aaattaataa
      421 atagattaaa tcccatttct cttggtgaat agtcacctct ttttaaacga ttaaaatcat
      481 acaaagtata aagtagaaat aacaacagac taacgacggt aataatagtg taaaaaatag
      541 gattttgaag aaaaatacca attagactcg caataattaa tgttattaat gtaacgaata
      601 aatatttgcc tatactcgat gcatctccaa cgaagaaata accaataata ccaaatgcta
      661 taaatgcaaa aattgctaat gcgatatttt tatagaaaat atctggtcct aaattttgta
      721 aatacgtggt aaacgttgcg tatgacaata agccaatcac tacagcgtaa atatgtgaaa
      781 taattaaacc gaaccgtctc gctctttcaa aaacaagtgt tgctaaaata ataatcagta
      841 atccaaatga caagggttgt cgccaactta atggtaaaaa ctgacctaga tagcatccta
      901 tgccaaatat aatccaataa tacataaaat aaagccacac tttacttatt gattgagcat
      961 gctggttttg atgataatat gaatgatttg tattttgcga caaaataatt cctccttcta
     1021 ccgtcgtttt atgcgtcaaa ttttacagta aattttgctt catcaaaaga aataaccttt
     1081 aacaagtata gtaattttac attttacaat gttacaaaat ataatttttt ataattagtt
     1141 aaaatcacta aaacgctttt atacactatc aaatcagcat ttataaaaat atgaaccgat
     1201 atcctaaaat gttaataata ttacaagata ataacaaacc acacaaagct acttattttt
     1261 gataatatgg aaatcgtaat ataaaacgaa aacttaattt actatataaa ttgtcttaat
     1321 aatttttaaa agtagtaaaa cataatttta aggaggagtc cctttgaaaa aattagcatt
     1381 tgcaataaca gcaacatctg gtgcagctgc atttttaacg catcatgatg cacaagcttc
     1441 tacacaacat acagtacaat ctggtgaatc attatggagt attgctcaaa aatacaacac
     1501 ttcagtagag agtattaaac aaaataacca attagataac aacttggtat tccctggtca
     1561 agttatctca gtaggtggaa gtgatgcaca aaatacgtca aacacttctc cacaagctgg
     1621 ttcagcatca tctcatactg tacaagctgg tgaatcatta aatatcattg ctagcagata
     1681 tggtgtttca gttgatcaat taatggcagc caataactta cgtggttatt taattatgcc
     1741 taaccaaaca ttacaaattc ctaatggtgg atcaggtggt acaacaccaa cagctacaac
     1801 aggtagcaat ggcaatgcat catcttttaa tcaccaaaat ttatacactg ctggtcaatg
     1861 tacatggtac gtatttgacc gtcgtgctca agctggtagt ccaattagca catattggtc
     1921 agacgctaag tattgggctg gtaacgcagc taatgatggt taccaagtaa acaacacacc
     1981 atcagttggt tcaattatgc aaagcacacc tggtccatat ggtcatgttg cttatgttga
     2041 acgtgtcaat ggtgatggta gtatcttgat ttctgaaatg aattacacat atggtccata
     2101 caatatgaac taccgtacaa ttccagcttc agaagtttct agctatgcat tcatccatta
     2161 attaaataaa ttgtactgat atatactagc aattcacatc atgtgagatt gctagttttt
     2221 tatttttgaa aaaaattttc attttggtac aaaaaattat ctcacccttc cctatcatac
     2281 atatttatat tttgtatgaa tggtagttag gtaaaaatta acaacctacc tatttgatta
     2341 aattaaatgc ataatatatt aaaaatcaaa ataatgcaac taagttgact atataatgat
     2401 ttaatttgtt ataccagtat tttacgcttt ttcgtctaca tatacaaatt tatattaaat
     2461 aaagcccaat acaatttagg ttaattaaac aagttgataa ctatttaatt attccttcat
     2521 tgaagaatat aaactattaa atcattattt tgctcttaca tatattttaa tgacctaact
     2581 gattatgttc catggaatac atttataata tagcctccta attaaaatgc tttgtcttgg
     2641 tcattctacg taaattctat aaaatatgtt atctacttac ataaaaaact gaacttcaat
     2701 accaccatat gtttgtgata ctgaagttca gtttagtttt attttcaatt agaaaaataa
     2761 gttaagtata tagaatagta aacctgctaa caatgctgaa ataggtaatg taatcaccca
     2821 tgtaatgatc attcgttgcg cagtgctcca ttttacacct ttagctcggt tagaagcacc
     2881 aacacctaag attgatgatg acacaacgtg agttgttgat aatgggaaat gtagcgatga
     2941 tgcaacaaaa attgttaatg cagatgataa atcggccgca gcaccatttg ctggacgtat
     3001 tttcataata ttaccaccta cagttttgat aattttccag ccaccaattg cagtaccaag
     3061 ccccattgct gtcgcacagg caaattttac ccataactgt ggttcaacac tgccatcatt
     3121 ctgtacatta gcgacaatca atgccaacgt aataataccc attgattttt gcgcatcatt
     3181 cgtaccgtga gagaatgatt gtaacgctgc tgtgaaaatt tggaaaaatc taaagttacg
     3241 attcgctctt gttaaatttg catttttaaa gataacttta aaaattgaat acatcaagaa
     3301 accaacacaa aatgcgataa tcggtgaaac gattaataca ataataattt ttgtgaaacc
     3361 ttggtaatgt aacactccaa atgagccttc agatgcgatt gctgcacccg caattgaacc
     3421 tataagtgca tgtgaagacg aacttggaat tccgtaaaac caagtagcta aattccaaat
     3481 aatagccgca agtattgcag ctaacacaac aactaatcca ttttccaatt taaatggatc
     3541 gacaatgtct ttagtaatgg tgcctgcaac gcccgtaaat gttaaagcac ctataaagtt
     3601 catcactgct gccattaaaa ttgccgtttt aggcgttaac gctctagtag atacagcagt
     3661 agctactgca ttggctgtat catggaatcc attgataaag tcaaatatca gcgagaaaat
     3721 aactacagct atagtgacga tgattatata tgacataaat atatactccc cttagctatt
     3781 tttcataata atagtttcaa aattatttgc tacgatttga catttatcag cgatttcttc
     3841 catgctttca taaatatctt ttattttaat taaagtgatt ggatctgttt cgctattgaa
     3901 aatatgttta attgactgtc ttaaaatacc atcacagttt gtttcaaatt ctttaatatt
     3961 aattgaatga atacgcatat gtgataattt tttatcgact aataagccga cagcaagttt
     4021 catttctgca actgcttttt gaatgttatc aacaaactca gccatatatt catctgtgta
     4081 ttcgattgaa tacatttcaa acatagctgc cgtttcttca attgcatcta aaacatcatc
     4141 aattgcatca cataatgata aaatatcttc acgttcaatt ggtgtgataa atgtttgatt
     4201 taaatcagta attacttgat gtactaattc gtcaccatgt gactcataag ttttaatgtt
     4261 gtctgagtat gcttttaaat ctaaatgtgt attgaaatcc attttaccga attcaatagc
     4321 agcacgatcc agattgaaaa ccatctcttc taattgaacc ataaacttat cttttttctt
     4381 actaaacatt taaaatcctc catttaagcg attgtcacca atcacattca gttataattt
     4441 gtttcaaatt aagacaagtg aatttacaaa ctaatgatac aaatttgtta ttatcaatcg
     4501 tcagtataat tttagtgtac tgatattaat ttcaaaaatg cctcacagta aacaatttac
     4561 tgtatttgcc cttatatgaa aatagtacaa aatttacaat cccacaattc aattt
//
LOCUS       DALAGF010000148         4043 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.150, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000148 DALAGF010000000
VERSION     DALAGF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.150"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..495
                     /locus_tag="O2692_002592"
     CDS             <1..495
                     /locus_tag="O2692_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCZ4300190.1"
                     /translation="GFEYRAQKEKYDNLYKFFKENEKKYQYTGFTKEAINKTQNVGYK
                     NEYFYITYSSRSLKEYRKYYEPLIRKNDKEFKEGMERARKEVNYAANTDAVATLFSTK
                     KNFTKDNTVDDVIELSDKLYNLKNKPDKSTITIQIGKPTINTKKAFYDDNRPIEYGVH
                     SKDE"
     gene            488..2514
                     /locus_tag="O2692_002593"
                     /pseudo
     CDS             488..2514
                     /locus_tag="O2692_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2557..3762)
                     /locus_tag="O2692_002594"
     CDS             complement(2557..3762)
                     /locus_tag="O2692_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCZ4300191.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIR
                     NIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPN
                     EYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIAS
                     ILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKR
                     SISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTK
                     ALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDREL
                     TQKLENIPS"
     gene            3888..>4043
                     /locus_tag="O2692_002595"
     CDS             3888..>4043
                     /locus_tag="O2692_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCZ4300192.1"
                     /translation="MTQFLGALLLTGVLGYIPYKYLTMIGLVSEKNKVINTPVLLIFS
                     IETCLIWF"
BASE COUNT         1500 a          501 c          668 g         1374 t
ORIGIN      
        1 ggctttgaat atcgagcgca aaaagaaaag tatgataact tatataaatt cttcaaagaa
       61 aatgaaaaga aatatcaata tacaggcttt acaaaagagg caattaacaa gacacaaaat
      121 gtcggatata aaaatgaata tttttatatt acatactctt ctagaagttt aaaagaatat
      181 cgaaagtatt atgaaccact gattcgaaaa aatgataaag aatttaaaga aggaatggaa
      241 cgagcaagaa aagaagtgaa ttacgctgca aatacagatg ctgttgctac acttttttct
      301 actaagaaaa actttactaa agacaataca gtagatgatg taatcgaact aagtgataaa
      361 ttatataatt taaaaaataa accagataaa tctacaatca caatacaaat agggaaaccc
      421 actattaata ctaagaaagc cttttatgat gataatcgtc caatagaata tggggtgcac
      481 agtaaagatg aataaaatta atgataggga tttaacagaa ttaagtagtt actgggttta
      541 tcaaaatatt gatataaaaa aagaatttaa agttaatgga aaaaggttta aacaagtaga
      601 cagttataat gatgataaga atagtaattt gaatggtgct gctgatatta aaatatatga
      661 gttattagat gataaaagta aaccaactgg tcaacagaca ataatttatc aaggaacatc
      721 taatgaggca attaatccaa ataatccatt aaaatcatcg gggtttggag atgattggct
      781 ccaaaatgct aaattaatga ataatgataa tgaaagcaca gattatttaa agcaaacaga
      841 tcaattatca aatcaatata aaataaagtt agaagatgca gatagattat caaatagtga
      901 ttttttaaaa aaatatagaa tggaatcaag taacttcaaa aacaaaacca ttgtggcgga
      961 tggcggtaat tcggaaggcg gtgcaggagc aaaatatcaa ggagcgaaac atccgaatga
     1021 aaaagttgtt gctactgact cagcaatgat tccttatgct gcttggcaga aatttgctag
     1081 accacgcttt gataatatga ttagttttaa tagtaccaac gatttattaa catggttaca
     1141 agatccattc atcaaagata tgccaggaaa acgcgttaac attaatgatg gtgtgcccag
     1201 gttagatact ttaatagaca gccatgtagg ttataaaagg aagttaaata gaaaagataa
     1261 cacatacgat actgtaccac taatcaaaat aaagtcggta aaagatacag aaattaaaaa
     1321 tggaaaaaag taaaaaagac tattaacata acattagata tggatgggcg aattccaata
     1381 aatgtttgga caggagattc gattgcacgt tctggaagag gaactttaat taaacttaat
     1441 ttagaaaatc ttgatgcgtt gagtaaactg attactggtg aaacaagtgg tatgttagca
     1501 gaatgcgtaa tctttttaaa tgaaagtttt aacatctcag aaaatgaaaa taaaaatttt
     1561 gcagatagaa agaaacaatt atcagaagga tttaaggata agattaactt atttcagtta
     1621 gaagaaatgg aaagaacttt aattagtaaa ataaactcac ttgaagaagt tgcagatgaa
     1681 acaatagaaa gtattagtgc tgttaaacac ttattacctg attttgcatt ggatgcatta
     1741 aaagaaagaa ttaatgagtt gtttaaaggt ataaaatctt ttatagaaaa agtgtatgat
     1801 agtatagata atgaaatttt agaaattttc aaaaatatag atcacgactt cagagatgga
     1861 gtatctgaag aaatgatgaa acatttgaaa gtagtgaaac agaatataga ccaaataaaa
     1921 aatcaaaatg atatttatgg taggcaaatt gcagatatta gaagtattat gaaacaacaa
     1981 gatgcaacaa ttttagatgg aaattttcaa attaattgta gcggcgaaaa tatggtacag
     2041 ggtctagtta taccttctaa ttatttagga agaaaaatga aaatattaaa agaccatatc
     2101 gatgatggta ttaaaaaaat agcagactat gttcaaggta tatatgatga atatgcatcg
     2161 aaaattgtcg atgtaataaa atatttgatt aatacaattc ccaaaatacg taagaattta
     2221 agacatgcaa ttgaaatgtt aaatgtaaaa aagaaagaat ttttgtccct gattcctaat
     2281 gtaacttgta attatattaa aactaaatta gaagaattag ataatacttt aggcaaatgg
     2341 gagccttttc ttaatgattt aaaagcagtg tcaccaattt tagataacca tttagatgat
     2401 attgttaaga acatgaagcc tttgattgta caaatgatat ttgaaccatc acattatgat
     2461 gatatgttta tttcaagaaa agctttaacg ccagtgttct caagcgtttt ataaagcttg
     2521 taaaaaatat aagggcaaaa aaagggcgga tttaagctaa cttggaatgt tttcgagttt
     2581 ttgagttagt tctctatcca ttttttcagt tacatgagta tatatgcgaa tggttgtttt
     2641 ttcatctaca tgtcctaccc ttttcataat tgcttttaaa gaaacattca tttctactaa
     2701 taaagttatg tgtgtatgtc taaatgtgtg cgtggtaact ttcttattca tatttaaagc
     2761 ttttgtagtt ttcttaagca caccggcgat ttgattatta cataaaggat tccctttttt
     2821 tgttgtgaat atgaaccctc tgtcaacata gctcgaattc catcttttca acattttgtt
     2881 ttccagtatt atctttttaa aaatttctac ggttctagaa ttgatgctga tacttctttt
     2941 tgaacttata gtctttgtag tgtctttgta tccgaatcct tcctcgtatt taatgcggtg
     3001 aattgtacct gttatattga tagttttgtt taataaatct atatcttttt cctgcagtgc
     3061 ttgtagttct cctatgcgca taccagttaa agcctgtact tctaagatgc tggcaattaa
     3121 aatgcgattt cgcttgtgta acttattatc atttagtata tgatcacgta tctgtaggac
     3181 ttggttcatt tctaaatagt tgtacatttt agattcatct ttttcgatat cctctattgt
     3241 ttttcttctt ttaggaattt tgacattagt taacaaatat tcatttggat aattgtaaaa
     3301 tttaactgca tatttaatag ctcctttcat atctccgagt tgacgggtta cttgattttg
     3361 agaatagata tctgataatt tattaataaa tatctgcata tattttgtat ctagtttgtt
     3421 taaaagcaag ttctcagagc tgtatcgttt aatgtttcta attcttattt ttatattatt
     3481 aagagtagtc aactttgaac ctgatgtttt tatatgatat tcaagccatt catctaatag
     3541 cgcgtgaaaa gtcaaagttt ttaattcgct tgacgacttg ttgttcagtt tttcttttat
     3601 tttttcttct aaacgaaaca ttgcttcttt ttgagattgt tttgtattct tgttcaacac
     3661 aacacttacg cgcttccatt tatctgtgta tggatcttta tacttctcgt agtatctgta
     3721 tttagtttcg ttatttttgt ttttaaattt ttcaatccac atgtttatac ctcctgcaag
     3781 aacgtatgtt ctataaaata ttaaaaaata ataagggtag tcgggctacc cgtaatttag
     3841 tactaggtac taaatgtgtt ataataaaat aaaaagtagg tgataagatg actcaatttc
     3901 taggggcgct tcttcttaca ggagttttag gttacatacc atataaatat ctaacaatga
     3961 taggtttagt tagtgaaaaa aacaaggtta tcaatactcc tgtattattg attttttcta
     4021 ttgaaacatg tttgatatgg ttt
//
LOCUS       DALAGF010000149         3981 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.152, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000149 DALAGF010000000
VERSION     DALAGF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.152"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            130..501
                     /locus_tag="O2692_002596"
     CDS             130..501
                     /locus_tag="O2692_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCZ4300193.1"
                     /translation="MLKKWLGMALITPILTFIIWLFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
     gene            complement(556..1800)
                     /gene="fabF"
                     /locus_tag="O2692_002597"
     CDS             complement(556..1800)
                     /gene="fabF"
                     /locus_tag="O2692_002597"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCZ4300194.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVNS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(1812..2753)
                     /locus_tag="O2692_002598"
     CDS             complement(1812..2753)
                     /locus_tag="O2692_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCZ4300195.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASLKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSDGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKIKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            3048..3233
                     /locus_tag="O2692_002599"
     CDS             3048..3233
                     /locus_tag="O2692_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCZ4300196.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
     gene            complement(3490..3924)
                     /locus_tag="O2692_002600"
     CDS             complement(3490..3924)
                     /locus_tag="O2692_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCZ4300197.1"
                     /translation="MKLKSFITVTLALGMIATTGATVAGNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
BASE COUNT         1232 a          745 c          584 g         1420 t
ORIGIN      
        1 gctttaaact aatgaagaat gattgatttt agaatttcaa taaagctgaa gaaattaaaa
       61 aatcattcct caatgtctga ttttaatgta aactatgcgt aagagttttc tataagtggg
      121 agtgatcaaa tgttgaaaaa atggcttgga atggcactaa taacgccaat attaacgttt
      181 attatatggc tttttaatag ccatactatc attacatatt taaatatatt gttttatgtt
      241 tcattaatta tttttattag catatttttg attttacttg tacaagaagg catttttgat
      301 gcgacaagtt atggttttag aagattaaag tatcaaatgt catcttcgaa aaagaagaag
      361 tctatctctg atgatccatt tttcaaccca caagaggtaa aaaaagagca ttactttgtt
      421 tctacatgga taatcccctt attattaata aatattttat attttattat gacgattgtg
      481 ctgtcgctca tcttagtata aagaaactga aaaaatcttc agtaactaaa acaacgttac
      541 tgaagatttt tatagttatg cttcaaattt cttgaatact aatactgcgt tatgtccacc
      601 gaatcctaag ctattactca ttgcataagt aatatcaagg tcttgcgctt catttggaac
      661 aatatccaaa tcacattctg gatctggtgt taccgcatga attgtcggtg cgactttaga
      721 gtctttaatt gaaagcgctg agaagattgc ttcaattcca cctgttgcac caagtaagtg
      781 accagtcatt gattttgttg agttaacttt taagtgttta gctgcttcac caaatgtatt
      841 tttaatagct ttaacttcat ttaagtcacc aacaggagta cttgtaccat gggcatttaa
      901 gtattgtaca tctttaggtt caataccagc atcatccata gctgcttgca ttgctctaga
      961 accaccttca ccttctggag ctggcgctgt aatatgataa gcatcacctg tagtaccata
     1021 gccaactatc tcagcataaa tattggcacc tcgagcttgt gctgattcta aagattcaat
     1081 tactaaaata ccagcacctt cacccataac aaaaccatct ctaccttctt ggaatggacg
     1141 acatgctgtt tcaatgtcat catttgtaga aagcgctcga cttgcactga aaccagcaat
     1201 tgccatatga gtaattggtg cttctgtacc accagtaatc attgcatctg catcaccgcg
     1261 ttgcacaatt ttaaatgctt ctccgattga atttgtacct gttgcacatg ctgtaactgt
     1321 tgcaccattt ggtccttttg cacctaagtc aattgatact tgcccagttg ccatatcagg
     1381 aattaacatt ggtacgaaaa atggactcac acgtcttggg cctttatcca ttaattgttt
     1441 atgtgcaatt tcaaatgttt ccataccacc gataccagaa ccaatccata caccgattcg
     1501 atctgcagta ttttcattga tatctaattg cgcatcttta acagcctctc tagctgctac
     1561 aattgcatat tgagtaaatc tatccatacg acgcgcttct tttttgtcga tatgatcttc
     1621 aatattaaag tttttaagtt ctcctgctaa gtgaacgcta taaggttcag tatcgatacg
     1681 tgtaatttta tcgataccat ttacgccttt tagagcattc tcccatgttg ttttgacatc
     1741 attaccgatt ggagaaaggg ctcccatacc tgtaataact actcttttat tttgactcat
     1801 tcgttatcct cctattttcc ccattttatt gtcattgcgc cccaagttag gccgccaccg
     1861 aatccgacaa gaacaattgt atcatcatct ttgattttac cattttttaa ttcttgatcg
     1921 atacttaaag gtattgacgc agctgaagta tttccatatt tatttacaga aacactcatt
     1981 ttgtcttttg aaatacctaa gcgttctcta gctgattcca taattctaat attagcttga
     2041 tgaggaataa ataaatctat atcatctgat gttaaattcg ctttttcaac tacacgtgtt
     2101 gatgcatcac ccataattct aacagcaaat ttaaatactt ctcgaccatt cattttcagt
     2161 ttaccagtat ctttatctaa atataaatgt ttaccacctg tgccatcaga acccatttca
     2221 taacttataa tacctctgcc atctgaaact tcaccgatga taaccgcacc tgcaccatct
     2281 ccaaatagaa ctgcagtaga acggtcagtt aaatctgtta ttttagataa tttatctgca
     2341 ccgacaacta aaatgttatg ataatctcca gattgaacat attgtttagc tgtaatcatt
     2401 gaatacataa atccagaaca tgctgcaagt tgatccatag aggcaacttt gcccgtccct
     2461 aaacgttctt gcaacatatt tgcgacagtt ggaaatggca tatctccagt tgctgtggca
     2521 acaattatca tatctatatc ttcgggctga ataccagcgt cagcgattgc ttttaaactt
     2581 gcttcatatg ctaaatctga agtatcttga tcatcatctg cccaatgtct ttctttaatt
     2641 ccagtcatct tagaaatcca ttcatcagat gtatctaaaa attgctcaaa ataggcattg
     2701 tcaataatct tttctggcgc atatgcacca aaacctttaa tacccacgtt catagttgaa
     2761 caccttctta ataaaatttt taataccagg tattaattta acatagatta ctttttaaac
     2821 tcaagtcaga ggtaggaata ttgactagtt aataaaattt taatgcttta ttatcgtcaa
     2881 atttcagtat gaaattggtt ttaatgcgct taatttagaa tagccattca gataaaatca
     2941 tttatttaaa ctgtataata tggtcaaaat atatgagatg ttactataca aatttctaac
     3001 tttcacttaa aataaaatat atataattaa agtggaggta gaatgacatg aaacacctcg
     3061 taacattctt ttgggcatta ttattaatgc aaatggttaa ctttgtactt aatagcctag
     3121 taggtggaga cagcttaaat gtagtgaatc caattatcat ggcagtattg ttcacgattt
     3181 tcacagcaat ttttgctgca gtaattaaac cgccgaaaga ttcatcacaa taactattta
     3241 gtataaaatg aaatatgtct ttcttattat tgaagctgcc cagataaagg cggcttttat
     3301 ttttggccat taatgttttg tattttgaat attttaaagc tcaatgacct ctaacccatc
     3361 acttttaaat ttagaaaaag ccttaataca aagatgtcat ttacgagtaa tctttgtatc
     3421 aaggcaatta ttttataatc atcactgtaa aattaaatgc ttcatgttga atttgataat
     3481 tttaagtgtt tatttagttt ttacattaat atcaatttgt ttaattgaat ttgaatcaaa
     3541 caagtttgct gtgtagctat ctttcttaag atccacttct tttttactac catctttcca
     3601 agtaatcgtg taaacggcag attttgcatt tcgaatcttc tcaggtgtta caccgcgttc
     3661 atcatataac aaagctttaa ctttatttcc taaatctaaa tatgataatt ggctatcttt
     3721 tggtaaattt aagtaagatt gattgaaagc cataatgcca tctactgcaa ttgtatatgg
     3781 aacattttgc atttctttag ctttttgagt tgccggtagt ttgtcctttt ctgctgcaga
     3841 tacctcatta cctgccacag tagcgccagt cgttgcgatc atgcccagtg ccaaagttac
     3901 agttataaat gattttaatt tcatataatc tctctccttt gtgagttaaa gtatcttaca
     3961 aaatcaatat aacacaaatt g
//
LOCUS       DALAGF010000150         3780 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.154, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000150 DALAGF010000000
VERSION     DALAGF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.154"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(12..245)
                     /gene="nrdH"
                     /locus_tag="O2692_002601"
     CDS             complement(12..245)
                     /gene="nrdH"
                     /locus_tag="O2692_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCZ4300198.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHHIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            443..1804
                     /locus_tag="O2692_002602"
     CDS             443..1804
                     /locus_tag="O2692_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCZ4300199.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            1801..2820
                     /locus_tag="O2692_002603"
     CDS             1801..2820
                     /locus_tag="O2692_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCZ4300200.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFGISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            2953..3615
                     /locus_tag="O2692_002604"
     CDS             2953..3615
                     /locus_tag="O2692_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCZ4300201.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
BASE COUNT         1184 a          532 c          703 g         1361 t
ORIGIN      
        1 ttagatgaaa attaatcttg gatatttaat actttgttga tttcatcaag atcaacatgg
       61 tacattggat tgccattcaa caaaataaac ggagttgaaa aagcatcaaa atctatcatt
      121 tcgtttcgat attgttgatt gttgatattt ctctcttcaa aatcaatgtg atgctcattt
      181 agataatttt ttacaaatgt acaaggtgga caatcattct gcgtataaac gattatttct
      241 gacatgtatc atgctccttc gtgctttatt ctaatgtaat atacacaatt atacattgaa
      301 attcaaattt gtgaacacat tgtgaactga cataaattat acacaatttc atagcgcata
      361 atgttttaaa aatacacatt atagcaatta taattaagtt cattgtgttg tcatcaaaaa
      421 aagaaaaagg tgatgttttt aaatggatac agttgaaatc agtcggtttt tgacagctat
      481 gactttagca gttcatatca tttttgcaac gattggtgtt ggtatgcctt taatgttcgc
      541 aattgcagaa tttttaggta ttcgcaaaaa tgatcttcaa tatatagcta tggccaaaag
      601 atgggctaaa gcttatacaa ttactgtagc agtgggagtt gttacaggta caattatagg
      661 acttcaatta tcattgattt ggcctacatt tatggaaatg ggtggacacg ttattgcact
      721 tccattattt atggaaacat ttgcgttctt ctttgaagct attttcttaa gtatatattt
      781 atatacttgg gatcgtttta aaaataaatg gacacatttc ttaattagta taccagtaat
      841 tattggtggc tctttctcag cattcttcat tacttcagtg aattcattta tgaatacgcc
      901 tgcaggtttt gagttgaaga atggaaagat ggtcaatgtt caacctatag aagcgatgtt
      961 taacccatcg tttatagttc gatcatttca cgtaattact acagccggta tgacgatggc
     1021 atttgttatt gcatcaatag cagcttttaa attattgcgt aatcgtcaac ctaaagatac
     1081 tgtctaccat aagaaagctt tgaaaatgtc tatgatagtt ggattctttt caacattact
     1141 ttctatgttg gcaggggatt tatctgcaaa atttttgcat aaattccaac ctgaaaaatt
     1201 agcagcttat gaatggcatt tcgatacatc ttcccatgcc aaattattat tatttggtgt
     1261 gttagatgaa aagactcagc aagttaaagg tgcgattgaa ttacctggac tattgagttt
     1321 cttagcagat aatagtgtca aaactaaagt acaagggtta aatgattttc caaaaagttt
     1381 acatccgcct atgatcgtcc attatttctt tgatttaatg gtaacgatgg gaattttatg
     1441 ttttgtcatt tcaggtgttt atgtcttaac tttaatgttt aaaaagctta gaaagttttc
     1501 tactcataaa tggatgcttt atggaatatt attaacaggc ccagcttcaa tgctagctat
     1561 agaatttgga tggttcttaa cagagatggg tagacagcct tggattgttc gtggttatat
     1621 gcgcgtggca gaagcagcaa cacaagcagg cggaataacc ttcgttacaa ttttatttgg
     1681 catattgtac atcattttaa tgtatacatg tgcatacgta ttaattcgta tgtttaaaaa
     1741 taaaccggcg tatgaagatg taaatcgttt agccaagaag caaggaggag aaatagaaaa
     1801 atgatttatg catttatagg tataacagtg ttatggttgt ttttattttg ctatatcatt
     1861 attgcttcta tagattttgg ggcaggtttc ttcgcattgc attcaaagtt aactggtgat
     1921 gaaaagaaaa ttaatcactt aatttcacgt tatttaaacc cagtttggga agttacgaat
     1981 gtattctttg ttttcttctt cgtaggattc gtaggtttct ttccagagtc aatcaaatat
     2041 ttaggcacgg tattgttaat accaggttca atagcactga ttatgatatc gttgagaaac
     2101 agtttttatg catttgaaaa ttatggtcaa gatacaaaat tagcatggat gatcatgtat
     2161 ggggtaagtg gattattaat tccagcttca ttatctactg ctttaactat tacagaaggt
     2221 ggctatatta atgttcgaaa caatgttatc gatctagatt gggtgcagtt attattaagc
     2281 ccatttgctt ggtctgtagt attcttggca attatttcag ttttatatat ttcatcagga
     2341 tttttgacat attatgctaa aaaagcaaat gacgaaccag catataattt aacaagacaa
     2401 tggcacatat ttttagggcc gccgatgatt atcatttgtt tattcgtatt tctatcatta
     2461 cgcattcaaa attctgaaca tttttattca gctgtttttg actattggtg gatgtttggt
     2521 ataagtttct tattctttgc attagcttca ttgttaacat tctttaagaa aaaacatggt
     2581 ttggcttttg tatttgttat tttacaaatg atgttcgcgt tctttggcta tggaattagt
     2641 aaattgccat accttttata tccgtttgta aaaattacag atgcatatgt taatccagaa
     2701 atgggctgga cattagtgat tgtctttatt ttaggtttac ttttattact tccatcgtta
     2761 atattattat taagattatt tgttttcgac aaagaatatg ttgaaggaaa gaaatcataa
     2821 gttgagacga taccccaact ttcatttact tgcattactg catttagata atttagttgg
     2881 ggtactcatt tataataatt ttaaatttat attttcaatg aattgatgaa aatcatttaa
     2941 tggagggcga gtatgggtaa agaatatgta gtcatcggtc taggtcgttt tggaggtagt
     3001 atcgttcgtg agttgaatgc attagacatg gatgtaatgg ccatcgacca tgatgaaaat
     3061 agagtgaatg aatatagtga tatcgctact catgcggttg ttgcagacac tacagatgaa
     3121 gcagttatga aaagtttagg tatccgtaac tttgatcatg tcattgtggc aattggtgaa
     3181 aatattcaat caagtacgtt gacgacttta attttaaaag agttaggtgt aaaaaaagta
     3241 actgctaaag cacaaaatga ttatcatgca aagattttaa ataaaattgg agcagatacg
     3301 gttgtgcacc ctgagcgtga tatgggtaga cgtattgcgc ataatgttgc gagtgcaagt
     3361 gtacttgatt atcttgagtt ggcagacgag cattctattg tagaattgaa agcaactgaa
     3421 aagatggcgg ggcagtctat cattgattta gatataagag cacaatatgg aattaacatt
     3481 attgcaatta aacgaggcaa agagttcatc atttcaccaa atccaaatat taatttagaa
     3541 ataggtgata ttttaatcat gattggacat gataatgatt taaatcgctt tgaaaaaaat
     3601 attgcgacga gataatcggg agtgggacag aaatgatatt ttctcaaaat ttatttcgtc
     3661 gtcccacccc aacttacatt gtctgtagaa attgggaatc caatttctct ttgttggggc
     3721 ccacacccca actcgcattg cctgtagaat ttcttttcga aattctctat gttggggccc
//
LOCUS       DALAGF010000151         3718 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.155, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000151 DALAGF010000000
VERSION     DALAGF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.155"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            29..490
                     /locus_tag="O2692_002605"
     CDS             29..490
                     /locus_tag="O2692_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCZ4300202.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            495..2696
                     /locus_tag="O2692_002606"
     CDS             495..2696
                     /locus_tag="O2692_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCZ4300203.1"
                     /translation="MLFVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSAWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTDKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEVNEQFKERVKEIVIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLVITKQIKI
                     KSIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPSFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIVFIISVYYIFWLLAKRKYILVTFW
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYS
                     ISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKGYSSNTL
                     MLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKK
                     VLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKN
                     NMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSSSYGNASGL"
     gene            2753..>3718
                     /gene="holA"
                     /locus_tag="O2692_002607"
     CDS             2753..>3718
                     /gene="holA"
                     /locus_tag="O2692_002607"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCZ4300204.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLILELFIL"
BASE COUNT         1370 a          463 c          604 g         1281 t
ORIGIN      
        1 taaattttaa gtatggaagg tgaacatctt ggaaagaatc aaatgggaag aatattttat
       61 ggcacaaagt catttgctag cattacgttc aacttgtcaa agattatctg taggtgcaac
      121 gattgttaag gataatcgta ttattgctgg tggttataat ggctctgtag ctggcgaggt
      181 gcattgtata gatgaaggat gtttaattga agatggacat tgtatcagaa cgatacatgc
      241 agaaatgaat gctttattac aatgtgcaaa acaaggtgta tctactgaag gtgcaacaat
      301 ctatgttact cattttccat gcctaaattg tacaaagtca attattcaag caggtataaa
      361 gcgtatctac tatgcagaag attatcataa ccatgaatat gcaactaaat tactcaaaca
      421 atctggtatt gaatttaaaa aaattccatt ttcaccagaa tatgttgcta aatatctgac
      481 taaaggttaa tgttttgctg tttgtcgcgt tatcaatgat tgtaggagtg ctttggaatt
      541 ctagcaaagt gctctctaca tttcttttca ttttactttt gtatattact tatcgtaaaa
      601 ataaaatcgt ttatgcccct atttctctct ttttaatcat tttctccgca tggtatttac
      661 attattcaca acaagcaata tttaattata tcaattatat tgaacgtaat tctcagttta
      721 atgagcgtgc tcaagtaatc caaattcaac gtcaaggtag tgacacatat aaaggtaggt
      781 tgagtttaaa aaatgaaata tatcctttct ttttaacaga taaaaagaat tttgatttaa
      841 agaaaattga aagtcgtaat tgtattgtta aaggacaatt caaagttaat gacaataagt
      901 ttgtaactct taaattacaa agtatagttg tacaaagctg cctagaatcg aaccggtcta
      961 atttaattga gaaacataaa cagtttataa tgaatcgaat ttatgattcg ggtattaagt
     1021 ttccggatcg tattatggca ttgattactg gtgacgtaaa agaagttaat gagcaattta
     1081 aggaacgtgt taaagagata gttatatatc atttgctggc agttagtggc tcgcatatag
     1141 ctgcaattgt atttttaatt taccaacctt taaaacgatt aaatttacct ttatttgtca
     1201 ttaaaggaat tacaatcatt gtattagctt tatttgctca atacacaaat tatgcaccta
     1261 gtgctgtaag agctataata atgacaactc ttgtactggt tattactaag caaattaaaa
     1321 taaagagtat tcagctatta gcatttgcat ttataattat gtttatttta aatccactag
     1381 ttgtttatga tattggattt caattttcat tcatcatttc attttttatt atgctacttt
     1441 ttcctttttt acagcaattg tcaaagttac aatcattatt cataattacg tttattgcac
     1501 aattagcttc atttatcgtt gccattccaa gctttcatca acttcaatgg gtgggatttt
     1561 tatctaattt gatttttgta ccgtactatt cgattatatt gtttccgcta tctattttat
     1621 tctttattac aagtcatttt attgtgggat taacgccgct aaattacttg gttgacctaa
     1681 gttttaattt tcatgactgg ttactagacc tattcacaag aatcaagcaa tcacattttt
     1741 ctgttcccaa gtttaatgat tggatattta tagtatttat aatttctgtt tattacatat
     1801 tttggttatt ggctaaacgt aaatatatat tggttacgtt ttggactata attattctga
     1861 cattattaat aacgtttcca acaaattcac atcacaaaat tacaatgtta aatgtggggc
     1921 agggagacag tattttatat gaaggcggta agaaccaaaa tgtcttgatt gatacaggtg
     1981 ggaaagtgat tgatgatact aaacaaccta gttattcaat ttctaaatat catattttac
     2041 caacgctaaa tgaaagaggg ataaatgaat tagagtatct aattttaaca catccacaca
     2101 atgaccatat tggtgaagtg gaatatatta ttagtcatat taaaattaaa catatagtga
     2161 tatacaataa gggatatagt agtaatacat tgatgttatt atcgaaatta agccataagt
     2221 acaacattaa acttatggat gtaagacaag ttagtagttt taaacttgga gatagtagtt
     2281 ttctattttt tgatagtttt attccaaata gccgagataa aaatgagtat tcgattatta
     2341 ctatgattac atatcaaaat aaaaaagttt tattaatggg cgatgctagt aaaaataatg
     2401 aatctttact actaaaaaaa tataacttgc cggagattga tattttaaaa gtaggacatc
     2461 atgggagcaa gacaagtagt tctaaagaat ttatagagat gattaagcct aaaataagtt
     2521 tgatttcttc tgggaagaac aatatgtatc atcttcctaa tatagaagtt gttaaacgat
     2581 tgcaaaggat tcgcagtcgc atttacaata gtcaacaaaa cggtcaagtt acaattgact
     2641 tagatgataa tttaaaagtt gattcaagct cttatggaaa tgcaagtggt ttatagatac
     2701 agaaacatga tataatctat tgatgtgtca aacgaatgaa agggtgtaat gaatgagcga
     2761 caatattgta gctatttatg gagatgtgcc tgaattggtt gaaaaacaaa gtgcagaaat
     2821 catatcacaa tttttgaaaa gtgatagaga tgactttaac tttgtgaaat ataatttata
     2881 cgaaacagag attgcaccaa ttgttgaaga aacattaaca ttgcctttct tttcagataa
     2941 aaaagcaatt ttggttaaaa atgcatatat atttacaggt gaaaaagcgc caaaagatat
     3001 ggctcataat gtagatcaat taatagaatt tattgaaaaa tatgatggcg aaaatttgat
     3061 tgtctttgag atatatcaaa ataaacttga tgaaagaaaa aagttaacta aaactctaaa
     3121 aaagcatgca aggcttaaaa aaatagagca aatgtctgaa gaagaaataa aaaaatggat
     3181 tcaaagtaaa ttaaatgaga atttcaaaga tatcaaaaga gatgcattag atttatttat
     3241 tgagttgaca ggtattaact ttaatattgt ctcacaagag atagaaaagt tgattttatt
     3301 tttaggcgat agaccaacaa ttaataagca ggatgttaac caaattatta atagaagttt
     3361 agagcaaaat gtatttttac tgactgaata cattcagaaa agaaagaaag aacaagcaat
     3421 tcatttagta aaagatttaa taactatgaa agaagaacca attaaattac ttgcactaat
     3481 tacaagtaat taccgattat tttatcaatg taagattctg agtcaaaaag gatatagtgg
     3541 acagcaaatt gctaaaacaa taggagtaca tccatacaga gtaaaattag cgttaggaca
     3601 agtaagacat tatcaacttg atgaattatt gaatattata gatgcttgtg cggaaactga
     3661 ttataaactt aaatcatcat atatggataa acagttaata ctggaattat tcattcta
//
LOCUS       DALAGF010000152         3704 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.156, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000152 DALAGF010000000
VERSION     DALAGF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.156"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..297
                     /locus_tag="O2692_002608"
     CDS             <1..297
                     /locus_tag="O2692_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCZ4300205.1"
                     /translation="PQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLNNLIEQDH
                     RHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIMLAS"
     gene            355..732
                     /locus_tag="O2692_002609"
                     /pseudo
     CDS             355..732
                     /locus_tag="O2692_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            729..1610
                     /locus_tag="O2692_002610"
                     /pseudo
     CDS             729..1610
                     /locus_tag="O2692_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1726..2655
                     /locus_tag="O2692_002611"
     CDS             1726..2655
                     /locus_tag="O2692_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4300206.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2648..3385
                     /locus_tag="O2692_002612"
     CDS             2648..3385
                     /locus_tag="O2692_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4300207.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3399..3602
                     /locus_tag="O2692_002613"
     CDS             3399..3602
                     /locus_tag="O2692_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300208.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLGGSVAKLNL"
     gene            3632..>3704
                     /locus_tag="O2692_002614"
     CDS             3632..>3704
                     /locus_tag="O2692_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCZ4300209.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRY"
BASE COUNT         1354 a          507 c          654 g         1189 t
ORIGIN      
        1 cctcaaaagg tagttacaga tcaggcacct tcaacgaagg cagcaatggc taaaataatt
       61 aaagctttta aacttaaacc tgactgtcat tgtacatcga aatatctgaa taacctcatt
      121 gagcaagatc accgtcatat taaagtaaga aagacaagat atcaaagtat caatacggca
      181 aagaatactt taaaaggtat tgaatgtatt tacgctctat ataaaaagaa ccgtaggtct
      241 cttcagatct acggattttc cccatgccac gaaattagca tcatgctagc aagttaaacg
      301 aacactgaca tgatatatta gtgattagct atatttttta ctttgcaaca gaaccagcca
      361 tttaatatga ctgttacaat tactaagatt caagcactat tacgccgcac gtatgatttc
      421 accactcaag ttaataattt aacagtagat gattgcgagt tactacttga tgcagcaaag
      481 cttaaattta atgaagaaac catagacctg acacacactg aattgcaaat catgcatagc
      541 ttattccaga acaagggcaa attcgtcagt cgtaatgcgt taatcgaaaa atgctgggaa
      601 tcagaacact tcattgatga caatacgctt gctgtcaaca ttacacgttt acgcaaaaag
      661 ctacaaaaac taggacttac agattttatc gaaacgaaga aaaatgtcgg atatcgtgta
      721 ggtgacgtat gaatttcttg aaatcaatcc aacgtgaaat ttatattgtc atccttactt
      781 gtgctttatt ctactttata ttattcatgt ataatgtgcc gatgcaagca tatatgattg
      841 gtctaggtat tgtattgttt gtttcaatga tttactgggg tatccattac atgaacttta
      901 aaaaagaata aacactacag gaacagaacg aagatttaag aatcgaaaac cataaacttc
      961 gtaatgactt tattaactac caaagtgagt tagagtcata ttttttaatg tgggtgcatc
     1021 aaatcaagac accgattaca gcatcaaagt tactgcttga agatcctgat gaacacgtga
     1081 tttctaacgt gcgtaatgaa gtcttacaaa ttgataatta cacgaatctt gcactgaact
     1141 atttgaaact catgaaccaa aagacataca tggtctttat ggaagtgagt gtagatgaat
     1201 taattcgacc actgattaaa cgttatgcga tacagtttat tcatcatcaa acaaagttgc
     1261 attatgagaa aagtgaagcg gttatattaa cagatgccaa atgggcatca attatgattg
     1321 agcaaatatt aaacaatgca ttaaaatatg cacgtggcaa agatgtgtgg attacgtttg
     1381 acgaaaatac aaagaaatta tcaatccgag ataacggtat cggcattaat gcgagtgatt
     1441 taccgaaaat ctttgataaa ggtttctcgg gctataacgg tagaataagt gagaaatcaa
     1501 gcggtattgg tttatatatt gtcagcacaa tctcaaagcg cttaggccat aaagtaagtg
     1561 ctacatctac attaaacgat ggttcaatat tcaccattca atttaaataa ctccaatctt
     1621 tcatttttgt aagattagcg gctgtttttg taagaataga taaaggcagc cttttctaat
     1681 cgcgcgtaag ataaaggtaa caacaaacta agaaaggtgc cagaggtgaa aaatttgaat
     1741 aatataattg aaataaataa cttaacaaag caatatggaa gtcagatagc agttaataat
     1801 ttgagtataa ctgttgaaag tggacaaatt tatggttttt taggaagaaa tggtgcaggc
     1861 aaaacaacta ctataagaat gcttttaggc ttaattaaac ctacgaaagg taatattaaa
     1921 atatttgaaa aagagttcga aagtaataaa actgaaattt taaaaaatat tggtgctatg
     1981 gttgaaacgc caggttttta tagtaatttg acagctaaag agaacttaac tataaatgct
     2041 aaaatattag gtatacaaaa agaaaatgca attgaagaag tgttagaaat agtaggacta
     2101 gcttctgaaa aagtaaaatt gtttaaaaat tattctttag gtatgaagca acgtttaggg
     2161 attgcgagag ctttattgca caatcccgaa ttattgatac ttgatgagcc gacgaatgga
     2221 ttagatccta ttggcataaa agaaattaga aagttgatta aggatctggc tcaatctaga
     2281 aatataacca ttttaatttc aagtcatata ttgtcagaaa tagagcagtt agtagacaag
     2341 gtgggtatta ttcataacgg agttttgtta gaagaaatta tgtatgaaga tttaaaaaaa
     2401 aagaacagac aatttataag tatatcagta tcagatgaag aaaaagttgc atgtttgttg
     2461 gaaactaact tcggaattta tgactataaa attaaagaaa atggcgaatt taaaatttat
     2521 tcacatattg atagacaaca agaaattaat agattattag tattaaacaa tattgatgtt
     2581 ttccagatga gagtgataga agataatcta gaaacttatt tcgaagactt agtaggaggt
     2641 ggcacaattg gataatctac tccgtacaga atttattaag ttaaaaagat catatatgtt
     2701 tctaataagt attttaggta catgcgtagc accttttatg gtggttgtag cttcttttat
     2761 tagtatgcgg acgaaagtac cgactcctaa tattgtattt aaacagttat ttgaagaagt
     2821 cagtttatat catatgctaa ttattggacc tcctctatat ggggttataa tagcctattt
     2881 gtttggaaga gaatatatag aagatacttt aaaaaactta ttaattatac ctatatctag
     2941 agaaagtttt ttagtaagta aaataatatt attattcttg tggattcaaa cactatctgt
     3001 tattgcatgg ttgctaactt taatgctagg tatgctattg cattttcaag ggttaagcca
     3061 gagtttaata attgattcat tctttacatt tgttattggc ggagtgttat tatggatatt
     3121 atctccacca ataattattt tgtcgatata ttttaaaaat tatgttccca caattgtttt
     3181 aaccataata attactttga taaatttaat gactgctaat tctgaacata gaggattatt
     3241 tccttgggct gcagttggag acatatctag aggaacatta cctaatactt atccatttct
     3301 attatcttta attatcataa ttgttacatc tatattcagt gtaattatat tattctcata
     3361 ctttaaaaaa actgatataa gttaaaagga ggagaaaaat gggttttata gagcagatta
     3421 aagcatttat tttaggtgta gttgaagggt tgacagaatt tgctccagta tcatcaacag
     3481 gtcatatgat tattgttgat gatatgtggt taaacactgc tgacgattta ggaagtgcgt
     3541 ctgcaactac ttttaaggtg gtaatacagt taggaggttc tgttgcaaag ttgaatttat
     3601 aatataattt taacaaaaag gagtcttctg tatgaactat ttcagatata aacaatttaa
     3661 caaggatgtt atcactgtag ccgttggcta ctatctaaga tatg
//
LOCUS       DALAGF010000153         3667 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.157, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000153 DALAGF010000000
VERSION     DALAGF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.157"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..935
                     /gene="lukS-PV"
                     /locus_tag="O2692_002615"
     CDS             <1..935
                     /gene="lukS-PV"
                     /locus_tag="O2692_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     S"
                     /protein_id="HCZ4300210.1"
                     /translation="KKRLLAATLSLGIITPIATSFHESKADNNIENIGDGAEVVKRTE
                     DTSSDKWGVTQNIQFDFVKDKKYNKDALILKMQGFINSKTTYYNYKNTDHIKAMRWPF
                     QYNIGLKTNDPNVDLINYLPKNKIDSVNVSQTLGYNIGGNFNSGPSTGGNGSFNYSKT
                     ISYNQQNYISEVERQNSKSVQWGIKANSFITSLGKMSGHDPNLFVGYKPYSQNPRDYF
                     VPDNELPPLVHSGFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGNSY
                     LEGSRIHNAFVNRNYTVKYEVNWKTHEIKVKGHN"
     gene            931..1914
                     /gene="lukF-PV"
                     /locus_tag="O2692_002616"
     CDS             931..1914
                     /gene="lukF-PV"
                     /locus_tag="O2692_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     F"
                     /protein_id="HCZ4300211.1"
                     /translation="MDMKKIVKSSVVTSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDSDKLKISQILTFNFIKDKSYDKDTLILKAAGNIYSGYTKPNPKDTISSQF
                     YWGSKYNISINSDSNDSVNVVDYAPKNQNEEFQVQQTVGYSYGGDINISNGLSGGGNG
                     SKSFSETINYKQESYRTSLDKRTNFKKIGWDVEAHKIMNNGWGPYGRDSYHSTYGNEM
                     FLGSRQSNLNAGQNFLEYHKMPVLSRGNFNPEFIGVLSRKQNAAKKSKITVTYQREMD
                     RYTNFWNQLHWIGNNYKDENRATHTSIYEVDWENHTVKLIDTQSKEKNPMS"
     gene            2163..2348
                     /locus_tag="O2692_002617"
     CDS             2163..2348
                     /locus_tag="O2692_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300212.1"
                     /translation="MFILNTQAHARLDQMAQQFEVVCNGLNENEGQAIQTMDQSASLI
                     RSNLIQVKEQLEKLAVY"
     gene            2439..3356
                     /locus_tag="O2692_002618"
     CDS             2439..3356
                     /locus_tag="O2692_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCZ4300213.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDVVEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGKGVEVFVHCDDH
                     DIVFNASIPFDKSIIESDSSLRSEDKGDDMSTLVGTVLSGFEYRTQKEKYDNLYKFFK
                     DNEEKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIHKNDKEFKE
                     GMEQARKEVNYAANTDTVTTLFSTKENFTKDNTVDDVIELSDKLYNFKNKPEKSTITI
                     QIGKPTINTKKAFYDDNDPIEYGVYRKDE"
     gene            3413..>3667
                     /locus_tag="O2692_002619"
     CDS             3413..>3667
                     /locus_tag="O2692_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCZ4300214.1"
                     /translation="MFKKAKLILIATLLLSGCSAMNNESKKDTNTETNTETKSVPEEM
                     EASKYVGQGFQPPAEKDAIEFVKKHRKEFEKVGEQFFKDNF"
BASE COUNT         1495 a          504 c          612 g         1056 t
ORIGIN      
        1 tcaaaaaaag actattagct gcaacattgt cgttaggaat aatcactcct attgctactt
       61 cgtttcatga atctaaagct gataacaata ttgagaatat tggtgatggc gctgaggtag
      121 tcaaaagaac agaagataca agtagcgata agtggggggt cacacaaaat attcagtttg
      181 attttgttaa agataaaaag tataacaaag acgctttgat tttaaaaatg caaggtttta
      241 tcaattcaaa gactacttat tacaattaca aaaacacaga tcatataaaa gcaatgaggt
      301 ggcctttcca atacaatatt ggtctcaaaa caaatgaccc caatgtagat ttaataaatt
      361 atctacctaa aaataaaata gattcagtaa atgttagtca aacattaggt tataacatag
      421 gtggtaattt taatagtggt ccatcaacag gaggtaatgg ttcatttaat tattcaaaaa
      481 caattagtta taatcaacaa aactatatca gtgaagtaga acgtcaaaat tcaaaaagtg
      541 ttcaatgggg aataaaagct aattcattta tcacatcatt aggtaaaatg tctggacatg
      601 atccaaattt atttgttgga tataaaccat atagtcaaaa tccgagagac tattttgttc
      661 cagacaatga attaccccca ttagtacaca gtggtttcaa tccttcattt attgcaactg
      721 tttctcatga aaaaggctca ggagatacaa gtgaatttga aataacgtat ggcagaaata
      781 tggatgttac tcatgctact agaagaacaa cacactatgg caatagttat ttagaaggat
      841 ctagaataca caacgcattt gtaaacagaa attacacagt taaatatgaa gtgaactgga
      901 aaactcatga aattaaagtg aaaggacata attgatatga aaaaaatagt caaatcatca
      961 gttgttacat caattgcatt gcttttgcta tccaatacag ttgatgcagc tcaacatatc
     1021 acacctgtaa gtgagaaaaa ggttgatgat aaaattactt tgtacaaaac aactgcaaca
     1081 tcagattccg ataagttaaa aatttctcag attttaactt ttaattttat taaagataaa
     1141 agttatgata aagatacatt aatactcaaa gctgctggaa acatttattc tggctataca
     1201 aagccaaatc caaaagacac tattagttct caattttatt ggggttctaa gtacaacatt
     1261 tcaattaatt cagattctaa tgactcagta aacgttgtag attatgcacc taaaaatcaa
     1321 aatgaagaat ttcaagtaca acaaacggta ggttattctt atggtggaga tattaatatc
     1381 tctaacggct tatcaggtgg aggtaatggt tcaaaatctt tttcagagac aattaactat
     1441 aaacaagaaa gctatagaac tagcttagat aaaagaacta atttcaaaaa aattggttgg
     1501 gatgttgaag cacataaaat tatgaataat ggttggggac catatggcag agatagttat
     1561 cattcaactt atggtaatga aatgttttta ggctcaagac aaagcaactt aaatgctgga
     1621 caaaacttct tggaatatca caaaatgcca gtgttatcca gaggtaactt caatccagaa
     1681 tttattggtg tcctatctcg aaaacaaaac gctgcaaaaa aatcgaaaat tactgttact
     1741 tatcaaagag aaatggatag atatacaaac ttttggaatc aacttcactg gataggtaat
     1801 aattataaag atgaaaatag agcaactcat acatcaattt atgaagttga ttgggaaaat
     1861 catacagtta aattaataga tactcaatct aaggaaaaaa atcctatgag ctaaacagat
     1921 agataatcaa aaaatcttaa atatgttaaa atttacaaac actttctttc tatattaggg
     1981 taaccacgtc ttaattgacg tggttatttt ttcagggcaa aaaaagggcg gattatttaa
     2041 ataaggacaa acacttgtgg aaaatttaaa aggttaaaaa taataaagaa cttggtataa
     2101 caagggtttt atacatttgc gtacaacgac gaaatgtcaa tttaccatca cattatgacg
     2161 atatgtttat tttaaacaca caagctcatg cgcgtcttga tcaaatggca caacagtttg
     2221 aagttgtttg taatggcttg aacgaaaatg aaggacaagc aattcaaacg atggatcaat
     2281 ctgcctctct aatacggtca aacttaattc aagttaaaga acaattagaa aaactagctg
     2341 tatactaatt aatttattaa atgctacttg ttttcattga gaataagtag ctttttcaaa
     2401 cataaaagtt ttacaaacac ataaatgggt gatgagctat gtttaaaaga actaaactaa
     2461 tcttaatagc aacattactg ctatcaggat gttcaactac caataacgaa tctaataaag
     2521 aaacaaaatc agtgccagaa gaaatggaag cttcaaaata tgtaggtcaa ggcttccaac
     2581 cacctgcaga aaaagatgtg gttgaatttg cgaaaaagca taaagataaa attgccaaac
     2641 gaggcgaaca attttttatg gataatttcg gtctaaaagt taaagctacc aatgttgtag
     2701 gtagtggaaa aggcgtagaa gtattcgtgc attgtgatga ccacgatatc gtatttaatg
     2761 cgagtattcc atttgataaa tcaataattg agagtgatag ctcattaaga agtgaggaca
     2821 aaggcgatga tatgagtact ttagttggta cagtgttgag tggctttgaa tatcgaacac
     2881 aaaaagaaaa gtatgacaat ttatataaat ttttcaaaga taatgaagag aaataccaat
     2941 atacaggctt tacaaaagaa gcaataaaca agacgcaaaa tgtcggatat aaaaatgaat
     3001 atttttatat cacttactcg tcaagaagct taaaagaata tcgtaagtat tacgaaccat
     3061 tgattcataa aaatgataaa gaatttaaag aaggaatgga acaagccaga aaagaagtga
     3121 attacgctgc taatacagat acagtaacaa cattgtttag tacaaaggaa aattttacta
     3181 aagacaatac agttgatgat gtaattgaac tgagtgataa actatataat tttaaaaata
     3241 agccagaaaa atctacaatt acaattcaaa taggaaagcc tactattaat actaaaaaag
     3301 ccttttatga tgataatgat ccaatagaat atggagtgta tcgtaaagat gaataaattt
     3361 ttttaaaatg ctttttaata ttctatataa acacataaat gggtgatgag ctatgtttaa
     3421 aaaggcgaaa ttaatcttaa tagcaacgct actgttatca ggatgttcag cgatgaataa
     3481 tgaatcaaaa aaagacacaa atacagaaac aaatacagaa acaaaatcag taccagaaga
     3541 aatggaagct tcaaaatatg taggacaagg ctttcaacca cctgcagaaa aagatgcgat
     3601 tgaatttgtg aagaagcatc gtaaagaatt tgaaaaagta ggtgaacaat tctttaaaga
     3661 taacttt
//
LOCUS       DALAGF010000154         3629 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.158, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000154 DALAGF010000000
VERSION     DALAGF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.158"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            113..379
                     /locus_tag="O2692_002620"
     CDS             113..379
                     /locus_tag="O2692_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300215.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            523..831
                     /locus_tag="O2692_002621"
     CDS             523..831
                     /locus_tag="O2692_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300216.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTAIADKHK
                     NQIAYIFTLCISLSAVFLMSSILSFEYDFIALAFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(939..1172)
                     /locus_tag="O2692_002622"
     CDS             complement(939..1172)
                     /locus_tag="O2692_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300217.1"
                     /translation="MLRSLCKHYRILINAIKVGIEMKYKISLAYNLAIIIGSLIILCI
                     LISRGYDIYVILIPILTILASLINLFCDIKKHK"
     gene            complement(1153..2346)
                     /locus_tag="O2692_002623"
     CDS             complement(1153..2346)
                     /locus_tag="O2692_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCZ4300218.1"
                     /translation="MLYLEVLKNRNFTYLLIGNFLRRSCFVLFSLQIIWFTVELTNQS
                     SLKLSMMVMSQTLPFIIFGIFGGAYSDKHNKKKILYLSDLILSLIIIIIPLLAITSNL
                     NYLTLLTISTAITIINCYTDPAFRAILPEIIDEEHLATSNALIDSLQRGSNIILPALI
                     GVIVILVGNVGVFFICSILLFLGFIFNALLKYTNNNMIDRHSKEDFSETWEFLKQSKE
                     IPFIIIIQFACILINTGLWRVVLPLFISNILKEGVGVYGLATSCLGIASLLMSLIMGL
                     LSEKRLIFKFSIGVLVWGIGLSIINVFPSVAILYIGATLLGLGQSIEGLTRSVAIQIK
                     TPNHLMGKVFSISSTSNYAADTLSLGLISIIIPLLSLSNIFILGGVIISILSLAGLKF
                     VKKSM"
     gene            complement(2730..>3629)
                     /gene="sasG"
                     /locus_tag="O2692_002624"
     CDS             complement(2730..>3629)
                     /gene="sasG"
                     /locus_tag="O2692_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
                     /protein_id="HCZ4300219.1"
                     /translation="IPFKKERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEI
                     ISKGESKEEITKDPVNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTG
                     KVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTIT
                     TPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSG
                     PVNPNNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTGLESTQ
                     KGLIFSSIIGIAGLMLLARRRKN"
BASE COUNT         1193 a          630 c          477 g         1329 t
ORIGIN      
        1 ataattaata ccgcgaccta tttaattgtt ttcgcgatga aaaaggttta gaattacaaa
       61 aataactatt ttaaatttag ttacaaatat attttagaaa ggatgtgaaa taatgaaagg
      121 ctctaaacaa atacttttga ttatgggcat tatatctctt attgttttat ttatttttac
      181 actattcatc atggcgcaat atgcaaaaca ttatgaacaa aaatccgaca gttccaacgc
      241 acatacttta aattcatcat cagccataat tgagcaacat actatgtcga atttagcgtc
      301 tctagattta tacgctcctg ttcgtaacat tacttctagt cgtgatattc atcccattta
      361 tttcatgaca aaagattaaa acttcctttt tcataacccc attcattcaa ctaataaatt
      421 aaaaaatgat aaaaacgtac tgttttttag aaacatacat atataagaaa cgcatccatt
      481 actaaaagac agtacattat attctatcag gaaggtgcaa caatgaccgt caacatcgta
      541 tttagtatcg tattctgcat aagtatggtt atattaggca tttatgtcgc aataactaaa
      601 gatttcacac taatctcttt tataaatcaa acggccattg cagataaaca caagaaccaa
      661 attgcatata tatttacgct atgtataagt ttgagtgcag tatttttaat gtcttcaatt
      721 ctaagctttg aatatgattt tattgcatta gcattcttat ttttaacgat agcattatta
      781 cttatcgcat tattctacgt ttgcttttat aaaattacaa aatacccata aatgtttgtg
      841 atagtgtgtt ggttagattt attgatgcgc tgtcagtaag taattgtttg cgaaaaaaat
      901 acaaccttgt acgaatacaa agttgcatta atatagaatc atttatgctt tttaatatca
      961 caaaacaaat tgattaaact agctaaaata gtcaaaattg gaatcaagat tacataaata
     1021 tcataacctc tagatattag tatacataat ataattagcg accctattat aattgctaaa
     1081 ttataagcca aactaatttt atatttcatt tcaattccca ccttaatagc atttattaaa
     1141 attctataat gtttacatag acttcttaac aaactttaac ccagctaagc taagtattga
     1201 aataattaca cctcctaata taaatatatt acttaagctt agtaaaggta taattatact
     1261 tatcagtcct aaagacaatg tatccgctgc ataattcgat gtagatgaga tactaaatac
     1321 tttccccatc aaatgatttg gcgtttttat ttgaatggca actgatcttg ttagtccctc
     1381 tatagattgt ccaagtccca ataatgttgc acctatatat aatatcgcca cacttggaaa
     1441 cacattaata atcgataagc caattcccca aactaaaaca ccaatactaa atttaaagat
     1501 taatcgcttt tctgacagca aacccataat caatgacatt aataaagatg ctatacctaa
     1561 acatgatgta gctagtccat atacaccaac gccctctttt aatatattgg aaataaacaa
     1621 tggtaataca acacgccaaa ggccagtatt aatcaatatg caggcaaatt ggatgattat
     1681 aataaatgga atttctttag attgtttcaa gaattcccaa gtttcagaaa aatcttcttt
     1741 tgagtgtcta tcaatcatgt tgttatttgt atattttaaa agtgcattaa aaataaatcc
     1801 taaaaatagc aatatactac aaataaaaaa gacgccaaca ttaccaacta gtattacaat
     1861 gacaccaatt aaagcaggta aaataatatt tgagcctctt tgcaaactat cgattaacgc
     1921 attacttgtt gctaaatgct cctcatcaat aatttcagga agaattgccc taaacgcagg
     1981 atccgtatag cagttaataa tggtgatagc tgtagatatg gttagaagcg tcagataatt
     2041 taaatttgat gttattgcaa gtaaaggaat aattattata atcaaactta gtataagatc
     2101 agatagataa agtattttct ttttattatg tttatcagaa tatgcgccac cgaaaatacc
     2161 aaatataata aatggaagtg tttgactcat aaccatcatt gataatttta aagatgattg
     2221 gtttgtcaat tcaacagtaa accaaattat ttgtaacgaa aacagcacaa aacaactccg
     2281 acgtaagaaa ttaccaatca ataaatatgt aaagtttcta tttttcaaaa cttctaaata
     2341 caacatattt atcacctctc ataaaaataa ttgaatgcat ccaccagctt ttttagacct
     2401 tcttctaaac tctctttatc caaagcgcaa ttaattctaa tataatttag tcagttaaat
     2461 atcaattatt tcgaaatata catactactt gaaacaccat acataacccc caaaatgact
     2521 actcagaggt tatattctac taattatgat tatattaaat atgaaaatat tatcaaaaaa
     2581 atcaaattta taacaaaaat acacccctta aagttaggtc tttcaatcca acttttgggg
     2641 tgtatatcat tctcatcata ttctaggttg tttttaacaa actaaatata gtgaatgcaa
     2701 atcaactatt atttaaatta tgaattattt taattctttc ttctacgagc caataacatt
     2761 aatccagcaa ttccaattat actactaaag atcaaacctt tttgcgtgct ttctaaacct
     2821 gtttttggta attctgctcg ttttttctct tgattagcta ctgattcttt agcaatttta
     2881 gattttttaa ctttatcatt tttatccatt gaatgaactg ggccatttgg ttttgctctg
     2941 tctttcgata atcctggatt gttaggattt actgggccac ttggatgagt tggtctgctc
     3001 ggcttctctg ggttttcagg tccttttgga tcttttggtt tctctccacc gaactctaca
     3061 atcttatcta ctggttgttt tgtgatctct tctgttggtt gaccctcgcc aactttttca
     3121 cctgttaatg ggttcactgt gattggtgtt gtgattgtct tacttcctgg ttgtccttct
     3181 tgtttcactc gctcttcacc aggttgtaat tttggattaa actcacgttt tgtttcaaac
     3241 ggtatctcta ctgtttttgt ttctggtgta cccgtttttg gtccgtgttt aatcacatca
     3301 tccactggct cttcgatcac ttttcctgtg tctggattct tgattcctgg tttacctggt
     3361 actttttccg tttgatctgt gggtaagttt ggatcaaaga tatctttatg accttgcggt
     3421 attttctcgc caccgaattc tgttaattca ttaactggat cttttgtgat ttcttctttc
     3481 gattcacctt tactaataat ttctccagtt aatggatttt ttagtgttgg cgtcgttatt
     3541 gtcttctcac ctttttgtcc ttctcttgtt actttttctg tccctggtgc taaatcagga
     3601 ttaaatttac gttctttctt gaatggaat
//
LOCUS       DALAGF010000155         3374 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.160, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000155 DALAGF010000000
VERSION     DALAGF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.160"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..365)
                     /locus_tag="O2692_002625"
     CDS             complement(<1..365)
                     /locus_tag="O2692_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMEK domain-containing protein"
                     /protein_id="HCZ4300220.1"
                     /translation="MKRSTNQEKFLDTLIRLNTKIEELGKINILNNHIYSEYFFRDLL
                     NIVYGYSLENHNKKQKNAPAFDLIDNTNKIIIQVTATCKKQKIEDTLKKEYLTNKMEE
                     GYRLKFIFIGNQNNNIKNK"
     gene            complement(1214..2413)
                     /locus_tag="O2692_002626"
     CDS             complement(1214..2413)
                     /locus_tag="O2692_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCZ4300221.1"
                     /translation="MSNTQKKNVPELRFLGFEGEWEEKKLGNLTTKIGSGKTPKGGSE
                     NYTNKGIPFLRSQNIRNGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRT
                     AINSIVETHANLNQHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFK
                     EIANLKIFTPTIFEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQELRF
                     KDENGEEYPEWENKFIKDIFIFENNRRKPITSSLREKGLYPYYGATGIIDYVKDYLFN
                     NEERLLIGEDGAKWGQFETSSFIANGQYWVNNHAHVVKSNDHNLFFMNYYLNFKELRA
                     FVTGNAPAKLTHANLCNINLKIPCLTEQDKVSALLKSIDNKMNNQMNRIELLKERKKG
                     LLQKMFI"
     gene            complement(2406..>3374)
                     /locus_tag="O2692_002627"
     CDS             complement(2406..>3374)
                     /locus_tag="O2692_002627"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCZ4300222.1"
                     /translation="GKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLR
                     VGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAV
                     IANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPH
                     GVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLF
                     IDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRY
                     VDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE"
BASE COUNT          973 a          576 c          451 g         1374 t
ORIGIN      
        1 ttcttatttt taatattatt attttgattt ccaataaata taaactttaa tcgataacct
       61 tcttccattt tatttgttaa atactctttt tttagagtgt cctctatttt ttgcttttta
      121 cacgttgcgg taacttgaat aataatttta ttcgtattat ctataagatc aaatgctggt
      181 gcattttttt gttttttatt atgattctca agtgaatacc catatacaat atttaacagg
      241 tctctaaaaa aatattcgga atatatatga ttatttaaaa tatttatctt tcctagttct
      301 tcaatcttag tattaagtct aattagtgta tcaagaaatt tttcttgatt cgttgatcgt
      361 ttcaagtttt aagtctcctt ttaaaatgaa tatttcactt cttataacta atatttaaac
      421 aaaatgatca cataataata tttaatttac aatatgccac tacagtgtca acattttata
      481 aacactattc atttgaacat ggtctaaaac gaataggaca ttgtaaatga ttaaatagtc
      541 tgtctctagt tgatgtatat ggtcatttgt tatgcagata ataattaatc taattaattt
      601 ttacgtttta gttacttggt tgtgattgag gctttttgta tctagttgta atattctcct
      661 tttttagaat cataataggt acttcttgaa atttttagaa ctttgcacat tgctgatact
      721 gaatattgat acgcattttg ttgaaagact tcattttttg tctcaaaatt agcactgctt
      781 gctttaaatc atcattttac attatcaatc gatgtttttc tttgcgtaat tttattaact
      841 ctttttcatc acttgttaaa atgtcttgat gattgaatga ctcagtattt tggttttgtt
      901 ttatctaatt tgagaaagtt aatgatgtta gatcatattc tcgtataatt tcgtttctaa
      961 gcttaccatt ttcataaagt ttaattattt taatttaaat tatttactaa aagctcttca
     1021 gtctcttgtc ataataaaat cgcctaccct cttagtttaa caatgtctat tctcatagaa
     1081 tttgtccaac taaatgtaga cgattcacta aacttctaaa aaataaaccc caaattataa
     1141 tagtctcaat attaatatat tacaatttat tcataggatt tttatcattt caattaatag
     1201 gttgttatca gggttaaata aacatttttt gtaatagtcc ttttttacgt tcttttaata
     1261 actcaattct attcatttga ttattcattt tattgtctat agattttaac aatgcactta
     1321 ctttatcttg ttcagtgaga caaggtattt taagatttat attgcataag ttcgcatgag
     1381 ttaatttagc tggtgcatta cctgtgacaa atgctcgtag ttctttaaaa tttaaataat
     1441 aattcataaa aaacaaatta tgatcattac ttttaactac atgcgcatga ttatttaccc
     1501 agtattgccc attagcaata aagctactcg tctcaaactg cccccatttt gcaccatctt
     1561 ctcctattag taatcgttct tcattattga ataaataatc ttttacgtaa tcaataattc
     1621 cagttgcacc atagtaaggg tataacccct tttctcttaa tgaagaagta attggttttc
     1681 ttctattatt ttcaaagata aagatgtctt ttatgaattt gttttcccac tctggatatt
     1741 cttcaccatt ctcatctttg aatcgcaatt cttgtgtgaa gattttctgc atatagcctt
     1801 ttttctgttg ttgaagcaat tcaagttttt gttcttctaa ttcaatttgt cggtcgagtt
     1861 tgctgaagaa cttgcctatt ttttgctgtt cttcaaatat agttggggtg aagattttta
     1921 aattagcaat ttctttgaag tttagacctt ctcgactacc tccactttgt gcaaggaaaa
     1981 ttttcctttt accttttctt gataatagat actgtccaaa aaaattataa taatactctt
     2041 ttttcaatct aataatacat acatgttgat ttaaattagc atgcgtttca actatcgaat
     2101 taatggctgt tctacctatt gatgctcctg taatatttaa aagaacatca ccatagtacg
     2161 ttctactatt tttcatctca tcatctatat ctttactaat ataaactaag tcattaagat
     2221 ttaatttacc atttctaata ttttgactcc ttaaaaatgg tatgcctttg tttgtatagt
     2281 tttcacttcc acctttggga gtctttccac tacctatttt ggtagtaaga ttccctaact
     2341 tcttctcttc ccattcgcct tcaaatccta ggaatctcaa ttctggcaca tttttctttt
     2401 gtgtattact catctttcaa caccccaagt tctttcaggt atgcattgat ttcttgctca
     2461 atttctgcga tttctttatc gatatttttc aaatcttgtt ggacttgatc taaatcaatc
     2521 ggtgcttctt cttcgaatgt atcgacatat ctcggtatat ttaggttgta atcgttatcg
     2581 gcaatctctt gtagtgtcgc gctgtagcta tatttatcaa ttgtttcctt acgcttatat
     2641 gtgtctataa tacgttcgac ttgggcatcg cttaaatgat tttgattttt tcctttttca
     2701 aaatcattgg atgcatcgat aaatagtacg ttgtcgtctt gttggcgaca ttttttaaat
     2761 actaaaatac atgttggaat acttgtccca tagaaaatat tcgctggcaa accaatcaca
     2821 gcttctaagt agttcttttc ttcaattaaa taacgacgaa tgacaccttc tgcagcacct
     2881 cggaataata caccatgtgg gagtacaacg gccatggtac cttcatcgtc taggtaatgt
     2941 accatgtgtt gaataaaggc aaagtctgct ttagacttag gcgcaagttt gccgtaacca
     3001 ctgaatcgtt cgtcattttc aaactttgaa tctgcagtcc atttcgcact atacggtggg
     3061 ttcgcaataa ccgcatcaaa tgtattgcct aaaaaggctg ggttttccaa tgtgtcatca
     3121 ttacggatat cgaagttctc ataacgcaca tcatgtaata acatattcat gcgtgctaag
     3181 ttgtatgtag tattgttacg ttcttgaccg aaataacgat acacttgtgt ttctttacca
     3241 acacgtaaca acagtgaacc tgaaccacat gttgggtcat acacgtgacg taatttatct
     3301 ttaccgtctg tgacaatctt cgccagtatc ttagatactt gttgtggtgt atagaactcg
     3361 cctgcttttt tacc
//
LOCUS       DALAGF010000156         3319 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.161, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000156 DALAGF010000000
VERSION     DALAGF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.161"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            221..727
                     /locus_tag="O2692_002628"
     CDS             221..727
                     /locus_tag="O2692_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300223.1"
                     /translation="MIIKILTILLLLCILSYLVTNRKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVV
                     LEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIF
                     NYRMRSYK"
     gene            986..1483
                     /gene="efb"
                     /locus_tag="O2692_002629"
     CDS             986..1483
                     /gene="efb"
                     /locus_tag="O2692_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HCZ4300224.1"
                     /translation="MKNKLIAKSLLTLAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVK"
     gene            1637..1987
                     /gene="scb"
                     /locus_tag="O2692_002630"
     CDS             1637..1987
                     /gene="scb"
                     /locus_tag="O2692_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HCZ4300225.1"
                     /translation="MKFKKYILTGTLALLLSSTGIATIEGNKADASSLDKYLTESQFH
                     DKRIAEELRTLLNKSNVYALAAGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVA
                     LEKIYKEIDEIINR"
     gene            complement(2640..2825)
                     /locus_tag="O2692_002631"
     CDS             complement(2640..2825)
                     /locus_tag="O2692_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300226.1"
                     /translation="MNKHINKKAAKLLEDYKKVQQRKSKETDRGCLKSVIMLIVLFFL
                     IIFGIVACSTNVNFFFQ"
BASE COUNT         1262 a          418 c          506 g         1133 t
ORIGIN      
        1 tgattaatat ataagttaaa aattatacta tttaattgaa ttatgcattt tcgaagtaaa
       61 cataattcaa cacttacatt tttaaataga taaaatagtg taaaatcacg tgccaagatt
      121 actatagagt aggcattgag tattattggc aatcacttaa gtgattgtta tttttcgtgg
      181 tcaataataa agatataaat aaatatattg aggtcaaacg atgataatta aaattttaac
      241 aattctatta ctactttgta tattgagcta tttggttaca aatagaaaga agccttttct
      301 gttcttaaag acactcttta tgggtgtggt atttatcttt ataggatata tttcactggc
      361 aatatctgcc gtaattattt atggtattat tcaatttatc acaattgatt ttggtagttt
      421 tttcttaatg ggtattatat tgatcttgat ttcaagtata ttccaattat ttatagttag
      481 attacttttt agaaaaaaga atgtcgattt gacagaggtt gtcgttttag agcatttaat
      541 tcaatggttc ttagtttact ttgcgatcta tcaagcagta aatgaaaaaa tggacattaa
      601 tgatattaat atcgacaatt tccaatctgt cttttttgac gtgtctaatt tgaatttagt
      661 aattctacca acgttaatca ttagctgggt cacaatattt aactatagaa tgagaagtta
      721 caaataaaat ctatgagatt ataccttcag acaccaacat tcaaatggtg tcttttttgt
      781 tgtgtggttt tatttttgaa attcgaaaaa gtagaggcat gaattttttg actagtgtat
      841 aagtgctgat gagtcacaag atagatagct atattttgtc tatattataa agtgtttata
      901 gttaattaat aattagttaa tttcaaaagt tgtataaata ggataactta ataaatgtaa
      961 gataataatt tggaggataa ttaacatgaa aaataaattg atagcaaaat ctttattaac
     1021 attagcggca ataggtatta ctacaactac aattgcgtca acagcagatg cgagcgaagg
     1081 atacggtcca agagaaaaga aaccagtgag tattaatcac aatatcgtag agtacaatga
     1141 tggtactttt aaatatcaat ctagaccaaa atttaactca acacctaaat atattaaatt
     1201 caaacatgac tataatattt tagaatttaa cgatggtaca ttcgaatatg gtgcacgtcc
     1261 acaatttaat aaaccagcag cgaaaactga tgcaactatt aaaaaagaac aaaaattgat
     1321 tcaagctcaa aatcttgtga gagaatttga aaaaacacat actgtcagtg cacacagaaa
     1381 agcacaaaag gcagtcaact tagtttcgtt tgaatacaaa gtgaagaaaa tggtcttaca
     1441 agagcgaatt gataatgtat taaaacaagg attagttaaa taaaacttca atcgttgctg
     1501 ttatctggaa ataattaatt aaatgttatg ttaatttttg ttaatgaaaa aagtaatcta
     1561 tttaatgaca ggttaatgta attgtcctga aattgactat atactcagta agtatcaatt
     1621 ttaaggagag cttataatga aatttaaaaa atatatatta acaggaacat tagcattact
     1681 tttatcatca actgggatag caactataga agggaataaa gcagatgcaa gtagtctgga
     1741 caaatattta actgaaagtc agtttcatga taaacgcata gcagaagaat taagaacttt
     1801 acttaacaaa tcgaatgtat atgcattagc tgcaggaagc ttaaatccat attataaacg
     1861 tacgattatg atgaatgaat atagagctaa agcggcactt aagaaaaatg atttcgtatc
     1921 aatggctgat gctaaagttg cattagaaaa aatatacaaa gaaattgatg aaattataaa
     1981 tagataataa ataaaacagg ttgagacaaa aaatggtctt aacctgtttt caatttgcat
     2041 atgtgataaa ttctatatca aaatgcttat gtataatgaa tgacatttaa aagtagggga
     2101 gacaaatata aatacaatag ttcctaggat tactctcaaa ataactatat caattattta
     2161 ctttgctctc ctatttttta aaatatctac atgtttaaac aatcaaaagt gtacaatatt
     2221 aaattatcat ttccagttct agtgctatat tggtagtagt tgactaaatg aaaataagct
     2281 tataacaagt tttttcaata ctcgtggggc cacaacagag agaaatagga tcaccaattc
     2341 caacaaacaa tgcaagttgg cgggggcccc aatatagaag ctggcgaaaa gtcagcttac
     2401 aaaaatgtgc aagttggcgg ggccccaaca tagagaaatt ggaaccccaa tttctacaga
     2461 caatgcaagt tggggtagga catcgataaa gaaatacttt ttctttagca attagtattt
     2521 cttatgcatg agctttactc atgtattcat tttttaagta cacattagct acagctaatg
     2581 ataaagaacc actacataat aaatcattag tggttcttta tcatttctat ctcactcttt
     2641 tactggaaga aaaagtttac gtttgtagaa catgccacaa taccaaaaat aattaagaaa
     2701 aataagacga taagcatgat gacacttttc aaacaacctc tatcagtttc tttcgatttt
     2761 ctttgttgaa cctttttata atcttcaagt agttttgcgg cttttttatt tatatgttta
     2821 ttcatgatgt tgactcctta taatatatgt ttaattcatt aaaatagttg aaaacatgac
     2881 ttgaaataaa gatataaagc taaagtaagg atgttcaatt gtttcaaatt ttatgaaagt
     2941 ataacgttaa atatgttaat ttgatgatga tatttgcttt ttattttcca aatggaattt
     3001 acttaaactg atgcattaaa atattaatga agcactagaa tacataaatg aatagtaatg
     3061 gtgcacagta tagaataatt aaggctatat taagtataaa tatcgttaac tgtaagctat
     3121 ctttagtttt aatataaact attaggataa tcgacgtaag aagaatcata tatattaatg
     3181 atgaagaagt ccatacaaaa tccgcatcat ttgttgttaa taatgggact ataattaatc
     3241 cgaaattaat catgcatgct atatatacta taatgttata cacaatgtta atttttgttc
     3301 accaccttat acttctatt
//
LOCUS       DALAGF010000157         3301 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.162, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000157 DALAGF010000000
VERSION     DALAGF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.162"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..1487
                     /locus_tag="O2692_002632"
     CDS             <1..1487
                     /locus_tag="O2692_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCZ4300227.1"
                     /translation="YFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRYLY
                     RNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRIYD
                     QERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLCEF
                     FYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGDLF
                     FSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYRAI
                     IVSTEQQKLDVEKRINHTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEKQL
                     HQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQEYS
                     DAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITSDFNGYLITKNDEDAL
                     FDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(1611..3083)
                     /locus_tag="O2692_002633"
     CDS             complement(1611..3083)
                     /locus_tag="O2692_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCZ4300228.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGAGNGIKSFYKTVFNNLTRYKAIVV
                     STEKQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(3202..>3301)
                     /locus_tag="O2692_002634"
     CDS             complement(3202..>3301)
                     /locus_tag="O2692_002634"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCZ4300229.1"
                     /translation="ENNGSNNATLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1085 a          507 c          490 g         1219 t
ORIGIN      
        1 attattttgt aggtaatagt ttaggtgtta atttaactgg gatagagaaa gcaattatta
       61 atcgtttaaa tttatttaaa gaaatgggaa gaccagcgca atgtgtattt ctgtcatgga
      121 atagatattt atatagaaat gcccaaaatt atattactag ttctgactat attaatatgt
      181 atgatttctt ccaagaagca acatatttag aacgcaatga accatttgac tggttgtcgt
      241 attggactga tgaatgtcat tatactttaa aacatgttga aaatagtcat gactttagaa
      301 tatatgatca agagcgtttc ttaatgtatg cccattttca agatcctaaa tatcgtatat
      361 tagattatgt aaatcatttt gatagtcaaa gacgaaaagt aaaacgtgat ttttatgatg
      421 tgcgtggctt tttaagttgt agtaggattc ttgttgataa acaacaaaca ttatgtgaat
      481 ttttctataa tccagaaggt gacacaaaat tagaaaagta tttttcttac aaggatggca
      541 aaccagaagt acaaaaaatt attgtttact atgctaacaa acaatatttc tttaataatg
      601 aaacggaatt aggcgcattt ttcattaaac aactatatca acatggggat ttatttttca
      661 gtgatcgtaa tgtgtacaca gcacctattt tcaacttaac gcctgagtca atcccagtag
      721 tagctgtgct tcacagtaca cacattaaaa atattgatgc attagattca tcgccattta
      781 aaaatgtata taaagcgatg tttgaaaatt tatctcgata tcgtgcaatt atcgtttcta
      841 ctgagcagca aaaattagat gttgaaaaac gtattaacca tactattcca gttgttaata
      901 ttcctgtcgg ctatagtgaa acaattgata caccagtcca aacattagat cagcgcagtg
      961 ttaaattaat ttccgttgct cgttattcac ctgaaaaaca attacatcaa caaattgaat
     1021 taattaaacg cttagtatcg tatgtcccaa aaatagagct tcatatgtat ggttttggtt
     1081 ctgaaagtaa aaaattaaat gaattgatac aaaaatatgg attggaaaat catgtttact
     1141 tacgcggctt tttaagcaat ctagaccaag aatatagcga tgcctatttg agtttaataa
     1201 caagtaacat ggaaggtttc tctttagcgc tacttgaatc tttagcacat ggtgttccag
     1261 ttattagtta tgatattaaa tatggaccta acgaactgat tacttcagat ttcaatggtt
     1321 atttaattac taaaaatgat gaagatgccc tatttgataa agtaaaatat gtgattgatc
     1381 atcctgaagt acagcaacga ctgtcaaaag gtagtttggc aaaagcacaa caatatagta
     1441 aagcgtcgtt aattaaacaa tgggaccaat ttgttcgtct tatataaatg tactttacct
     1501 ttatttttac aaaaatagca ttttcctatg tcatttaact aaacatgtaa gttcgtatga
     1561 acgaggtttg ttaaatagat gattctagga aaatgctttt ttcttttgac ttagtttaaa
     1621 atattttgcc actttgtact gatagtagtt gcattgtact gttgtgcaga ttctatgcta
     1681 ttaattgaaa actgttgcaa tttttgagta ttatttagta attggtcgac cttttcaacc
     1741 atttgattga tgtcaccttg aggtactaaa tagccattaa atccatcttg aatcagttct
     1801 gatggaccat aatctacatc ataactgatc actggtgtac ctactgaaag cgactctaaa
     1861 attgctaagc caaaaccttc cattttactt gtcgataaca tcagttctgc tttagcaatc
     1921 tcttcattaa tatgcgtctt aaaaccatga aatttaacat gttccgataa atgataatct
     1981 tctacaagtt gtcgatattc tgacaaacca tttccatgtc cataaatatt caattgaata
     2041 ttgggatgtt ttgttactaa ttgcttgatt acttcaattt gatgtttaat ttgtttattt
     2101 tcaacgaggc gagcaattga tatgatatga tttttctcct tttgattgat gtcaaattga
     2161 tactttaaat ttgccacgta gccaaccgga atattgataa ctggtatttt attttcaata
     2221 tattgtgaaa tatcttggca ttgcttttct gttgatacaa caatcgcttt ataacgtgtt
     2281 aaattattaa acactgtttt ataaaaactt tttataccat taccggcacc ggataaatgt
     2341 gtactatgga gtacaacaat aactggaata ctttgattta atcccgctat aacatttcct
     2401 aattcatgag gacgatctaa tatgatttga tcattatttt tacataattg atggagaaaa
     2461 tattgaacta attcatcttc tgtatcaaaa aattgttgat gctggtcttc atttaagata
     2521 acctttgtga gcgtgttttt cccttttata tcgtcaaaat atttttggat gacgatttcc
     2581 ccattaggtg tatagtaatt ttcgagtaca atccgttgtc cttcacctaa aattcgagaa
     2641 caacttaaaa agcctcttcc atcatacaat tcgcgtttta cttttcttct tttatgatca
     2701 aaataattca cataatttaa ttgatgatac tgtttatcta aaaaatgagc atacattaca
     2761 aattgctctt catcatatat tctgacatca tttgaatttt ccacaaattt caatgtgtac
     2821 ctacatgact tttcccaata ctgtatccag ttaacttgct ttgtcttttt ataattgatt
     2881 gctttttgaa aatagtcata cattgtaaat acatcatttt caatctgatg ttgcttcgca
     2941 tatgtgtatg aataaggatt ccatttaaca tatacacatt ttgaagatat gccgtgttgt
     3001 ttgaacaact tcaatctatt tatttgcgct ttttctacac ctgtaatttt actttctaaa
     3061 attgttccta aaatgtaatt catattatcg cctcatataa gttttattcc gtatctttat
     3121 tgtttatttt atatgaaaaa tacatctatt gcatgtgtaa ttataaaaaa accaggccac
     3181 aaggacctgg gtcatattgt attatttgtt ttgttttttg cgacgaccga ataacaataa
     3241 tgaacctaat gctgcaaata atccaccaaa taacgttgcg ttatttgagc cgttattttc
     3301 a
//
LOCUS       DALAGF010000158         3300 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.163, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000158 DALAGF010000000
VERSION     DALAGF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.163"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            42..>3300
                     /locus_tag="O2692_002635"
     CDS             42..>3300
                     /locus_tag="O2692_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HCZ4300230.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKLIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDL
                     QENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNDETLVDNNSNSNNENNADII
                     LPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLS
                     LKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQ
                     YPDYVVNEKNSIGSAFTETVSHVGNKENPGYYKQTIYVNPSENSLTNAKLKVQAYHSS
                     YPNNIGQINKDVTDIKIYQVPKGYTLNKGYDVNTKELTDVTNQYLQKITYGDNNSAVI
                     DFGNADSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVTLLNEKGEVIGTTKTDENGKYCFDNLDSGKYKVIFEKPAGLTQT"
BASE COUNT         1387 a          463 c          571 g          879 t
ORIGIN      
        1 aattgtatta tttaaaaatt tgatgaatta ggagtaatct aatgctaaac agagaaaata
       61 aaacggcaat aacaaggaaa ggcatggtat ccaatcgatt aaataaattt tcgattagaa
      121 agtacacagt gggaacagca tcaattttag taggtacaac attaattttt ggtctgggga
      181 accaagaagc aaaggctgca gaaagtacta ataaagaatt gaacgaagcg acaacttcag
      241 caagtgataa tcaatcgagt gataaagttg atatgcagca actaaatcaa gaagacaata
      301 ctaaaaatga taatcaaaaa gaaatggtat catctcaagg taatgaaacg acttcaaatg
      361 ggaataaatt aatagaaaaa gaaagtgtac aatctaccac tggaaataaa gttgaagttt
      421 caactgccaa atcagatgag caagcttcac caaaatctac gaatgaagat ttaaacacta
      481 aacaaactat aagtaatcaa gaagcgttac aacctgattt gcaagagaat aaatcagtgg
      541 taaatgttca accaactaat gaggaaaaca aaaaggtaga tgccaaaact gaatcaacta
      601 cattaaatgt taaaagtgat gctatcaaga gtaatgatga aactcttgtt gataacaata
      661 gtaattcaaa taatgaaaat aatgcagata tcattttgcc aaaaagtaca gcacctaaac
      721 gtttgaatac aagaatgcgt atagcagcag tacagccatc atcaacagag gctaaaaatg
      781 ttaatgattt aatcacatca aatacaacat taactgtcgt tgatgcagat aaaaacaata
      841 aaatcgtacc agcccaagat tatttatcat taaaatcaca aattacagtt gatgacaaag
      901 ttaaatcagg tgattatttc acaattaaat actcagatac agtacaagta tatggattga
      961 atccggaaga tattaaaaat attggtgata ttaaagatcc aaataatggt gaaacaattg
     1021 cgactgcaaa acatgatact gcaaataatt taattacata tacatttaca gattatgttg
     1081 atcgatttaa ttctgtacaa atgggaatta attattcaat ttatatggat gctgatacaa
     1141 ttcctgttag taaaaacgat gttgagttta atgttacgat aggtaatact acaacaaaaa
     1201 caactgctaa cattcaatat ccagattatg ttgtaaatga gaaaaattca attggatcag
     1261 cgttcactga aacagtttca catgttggaa ataaagaaaa tccagggtac tataaacaaa
     1321 cgatttatgt aaatccatcg gaaaattctt taacaaatgc caaactaaaa gttcaagctt
     1381 accactcaag ttatcctaat aatatcgggc aaataaataa agatgtaaca gatataaaaa
     1441 tatatcaagt tcctaaaggt tatacattaa ataaaggata cgatgtgaat actaaagagc
     1501 ttacagatgt aacaaatcaa tacttgcaga aaattacata tggcgacaac aatagcgctg
     1561 ttattgattt tggaaatgca gattctgctt atgttgtaat ggttaataca aaattccaat
     1621 atacaaatag cgaaagccca acacttgttc aaatggctac tttatcttca acaggtaata
     1681 aatccgtttc tactggcaat gctttaggat ttactaataa ccaaagtggc ggagctggtc
     1741 aagaagtata taaaattggt aactacgtat gggaagatac taataaaaac ggtgttcaag
     1801 aattaggaga aaaaggcgtt ggcaatgtaa ctgtaactgt atttgataat aatacaaata
     1861 caaaagtagg agaagcagtt actaaagaag atgggtcata cttgattcca aacttaccta
     1921 atggagatta ccgtgtagaa ttttcaaact taccaaaagg ttatgaagta accccttcaa
     1981 aacaaggtaa taacgaagaa ttagattcaa acggcttatc ttcagttatt acagttaatg
     2041 gcaaagataa cttatctgca gacttaggta tttacaaacc taaatacaac ttaggtgact
     2101 atgtctggga agatacaaat aaaaatggta tccaagacca agatgaaaaa ggtatatctg
     2161 gcgtaacggt aacattaaaa gatgaaaacg gtaacgtgtt aaaaacagtt acaacagacg
     2221 ctgatggcaa atataaattt actgatttag ataatggtaa ttataaagtt gaatttacta
     2281 caccagaagg ctatacaccg actacagtaa catctggtag cgacattgaa aaagactcta
     2341 atggtttaac aacaacaggt gttattaatg gtgctgataa catgacatta gatagtggat
     2401 tctacaaaac accaaaatat aatttaggta attatgtatg ggaagataca aataaagatg
     2461 gtaagcagga ttcaactgaa aaaggtattt caggcgtaac agttacattg aaaaatgaaa
     2521 acggtgaagt tttacaaaca actaaaacag ataaagatgg taaatatcaa tttactggat
     2581 tagaaaatgg aacttataaa gttgaattcg aaacaccatc aggttacaca ccaacacaag
     2641 taggttcagg aactgatgaa ggtatagatt caaatggtac atcaacaaca ggtgtcatta
     2701 aagataaaga taacgatact attgactctg gtttctacaa accgacttac aacttaggtg
     2761 actatgtatg ggaagataca aataaaaacg gtgttcaaga taaagatgaa aagggcattt
     2821 caggtgtaac agttacgtta aaagatgaaa acgacaaagt tttaaaaaca gttacaacag
     2881 atgaaaatgg taaatatcaa ttcactgatt taaacaatgg aacttataaa gttgaattcg
     2941 agacaccatc aggttataca ccaacttcag taacttctgg aaatgatact gaaaaagatt
     3001 ctaatggttt aacaacaaca ggtgtcatta aagatgcaga taacatgaca ttagacagtg
     3061 gtttctataa aacaccaaaa tatagtttag gtgattatgt ttggtacgac agtaataaag
     3121 acggcaaaca agattcaact gaaaaaggta tcaaagatgt taaagttact ttattaaatg
     3181 aaaaaggcga agtaattgga acaactaaaa cagatgaaaa tggtaaatac tgctttgata
     3241 atttagatag cggtaaatac aaagttattt ttgaaaagcc tgctggctta acacaaacag
//
LOCUS       DALAGF010000159         3258 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.165, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000159 DALAGF010000000
VERSION     DALAGF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.165"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            110..649
                     /locus_tag="O2692_002636"
     CDS             110..649
                     /locus_tag="O2692_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300231.1"
                     /translation="MVAYASHLSFLLFSNKKVLKINAYTGTDMAKLDLNSLDDEHVKL
                     LINELKYPETHIDVNELKTIVASRINERQEIISFKLGIKYLLTIKRGNIEKDRFSISI
                     IFKDTYHTLVRIDINGGTHDNPDGTIAPKSHIHIYNDKYDKKDRFAYEINLKDFPDIY
                     NLYNVYMSFLEYNNIKDLE"
     gene            662..1449
                     /locus_tag="O2692_002637"
                     /pseudo
     CDS             662..1449
                     /locus_tag="O2692_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
     gene            1910..2118
                     /locus_tag="O2692_002638"
                     /pseudo
     CDS             1910..2118
                     /locus_tag="O2692_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2692..2780)
                     /locus_tag="O2692_002639"
     tRNA            complement(2692..2780)
                     /locus_tag="O2692_002639"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2743..2745),aa:Ser,seq:gga)
     gene            complement(2835..2906)
                     /locus_tag="O2692_002640"
     tRNA            complement(2835..2906)
                     /locus_tag="O2692_002640"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2871..2873),aa:Glu,seq:ttc)
     gene            complement(2908..2982)
                     /locus_tag="O2692_002641"
     tRNA            complement(2908..2982)
                     /locus_tag="O2692_002641"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2948..2950),aa:Asn,seq:gtt)
     gene            complement(2997..3070)
                     /locus_tag="O2692_002642"
     tRNA            complement(2997..3070)
                     /locus_tag="O2692_002642"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3036..3038),aa:Gly,seq:tcc)
     gene            complement(3086..3161)
                     /locus_tag="O2692_002643"
     tRNA            complement(3086..3161)
                     /locus_tag="O2692_002643"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3126..3128),aa:His,seq:gtg)
     gene            complement(3175..3247)
                     /locus_tag="O2692_002644"
     tRNA            complement(3175..3247)
                     /locus_tag="O2692_002644"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3212..3214),aa:Phe,seq:gaa)
BASE COUNT         1255 a          496 c          504 g         1003 t
ORIGIN      
        1 acttaatttt aaaaatcccc tatgaaaatg ttgatgagct acatctttaa atcttattat
       61 acattgtata aaattatatt gcgaggtagt aaattgatat tatacacttt tggttgcgta
      121 tgcatctcac ctatcttttt tgcttttttc aaataagaaa gtattaaaaa ttaatgccta
      181 tacaggaaca gacatggcta aacttgattt aaatagtctt gacgacgagc acgtaaaatt
      241 attaataaat gaattaaaat atccagaaac tcatatcgat gtaaatgaat taaaaacaat
      301 agttgctagt cgaataaatg aaaggcaaga aataataagt tttaagttag gaataaagta
      361 cttattaaca ataaaaagag ggaacataga aaaagatagg ttttcaattt caatcatttt
      421 caaagatacc tatcacaccc tagttagaat agatattaac ggtggtactc acgataatcc
      481 agatggaaca atcgctccga aaagtcatat tcacatatat aatgataagt atgataaaaa
      541 ggataggttt gcttatgaaa ttaacttgaa agatttcccc gacatctata acttgtataa
      601 tgtgtatatg tcgtttttag agtataataa tataaaagac cttgaataaa ggagggataa
      661 tatgactaca tttgatgcta aaaaattaaa aaaagaatat cttgattggt ataatcagac
      721 cttagagttt tctaatttat caaacaatgt agtaagaata gatactcctt ttaaagataa
      781 ttctttagat aatttaataa tttacgcttt atacgatcag tccagagaca tgattacact
      841 gacagatgac ggctatacta tatttgattt agaaaataat ggtatttctt taaataaatc
      901 aaaaaaacgt aaaaagattt ttgaagagca cctttcagct tacggtatta aatataacga
      961 taaaactcac gaaatttttg ttcaaactaa ctttaaaaat tttaataaat cgaaacataa
     1021 tttattacag tgccttatat ttgttaatga tatgtactta ctttctaatc ctaagtcaca
     1081 gaacatattt acagaagatg ttgcaaacaa attggatgaa cataacattt attacggaag
     1141 agatttacct attataggaa gcagtggtgt tgttcataat ttcgactttt ttattagcgc
     1201 taagaaaaat caaaaagaaa aatttatcaa tgctatttct aaccctaata attctatgat
     1261 tattaagtcg aaaataacgg atgctatgca agcaaaaaaa taaaaagaca caggcaaaat
     1321 gagtttattt ttattttaaa tgactcaaaa aaagaaataa atgaacataa taaaaatctt
     1381 cttcatgaaa actatattag tacaatagat tatagcgaat tagatgaaaa gataggttta
     1441 ttgatttaat atatatagac gtgataatat caatgtttat attaaatgaa acgaatttga
     1501 aaatttcgaa acagcttaag atagcaaatt gaatagcctt attgataatg cagaatcgtc
     1561 tacacttagt tgaacaaatt ctatgagaat agatattgtt aaactatttg ggtaggcgat
     1621 tttattttga caagagtcag aagattattt agttcatagt ttaagttaca aatggctaaa
     1681 ctttatgaaa atggtatgac tagaatcgaa attatactcg aatacgattt aacactctca
     1741 atcttctcga actgaataaa tactgggtca ttcaatcatc aagacaactt aataagtgat
     1801 gataaatgtt cataccattt tgcaaatata accagatact ctcaacaaaa tgtagagttc
     1861 aaaattaaaa atgtccctaa ttggcacatt gctgtatgat aataaattca acttagattt
     1921 caacaatata actaatgatg cttatattta ccgcgatatc aaaccaaaca gcccatccac
     1981 aaacaaatta gaatatatca tgttaataat agtaaatgat atatcttcca aagaccgcaa
     2041 actgtagcag aacatgagaa ccttgcgatt ttgaaatggt ctaataaatc aaatatcatt
     2101 gaaactttaa tcacataaat taacagtagt cacctgttcg cagttctata aattacaaat
     2161 gcaataaagg ctaaagtaaa ctaattactt tagcctttat taataattta gtcgaattta
     2221 ttaaaaatcg actaactcct ggttatgcta cgctttactt tataatccgc acgtagatgc
     2281 cgattgatta tattataata ttattatttt attttttcaa gcgtatatat aatatttgat
     2341 tttctttaag taacagctaa acattacatt tcaaaacgat taggtcatgc agatgtacat
     2401 acaacgctta aaacatattc acatttgatt aaagaatttg aagataatga aactaaattt
     2461 atagaaaaaa atctgaatga tttgttttgt gactaaactt caaaatcttg tgactaaaat
     2521 taggattatt catcaaaaat tagtcacatt gtggctaaaa aattttaaat ggatataaat
     2581 aaataagaag tgtaaaaatg aggataatag ctcaaatgca gatatatcaa gggattgagt
     2641 ccaaaataaa aatagcgctt aaaatgggta aaaccaattt aagcgccttt acggaaggta
     2701 agggattcga acccttgaga cgcttgtgac gcctacacac tttccaggcg tgctccttcg
     2761 gccagctcgg acaaccttcc tcaacaataa atatgtaata aaaaaacaga agcgatattt
     2821 cacttctgct tgtatgactc ctacgggact cgaacccgtg ttaccgccgt gaaagggcgg
     2881 tgtcttaacc gcttgaccaa ggagccatgg ctccacaggt aggactcgaa cctacgaccg
     2941 atcggttaac agccgatagc tctaccactg agctactgtg gaataataga aaataatgga
     3001 gcgggtgatg ggaatcgaac ccacaacatc agcttggaag gctgaggttt tgccattaaa
     3061 ctacacccgc taatgtaaac gattatgggg cggctgaagg ggatcgaacc ctcgaatgtc
     3121 ggaaccacaa tccgatgtgt taaccacttc accacaaccg ccatgaatta agaatggttc
     3181 aggacagagt cgaactgccg acacatggag cttcaatcca ttgctctacc aactgagcta
     3241 ctgaaccgta atggtaat
//
LOCUS       DALAGF010000160         3244 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.166, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000160 DALAGF010000000
VERSION     DALAGF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.166"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            113..1399
                     /gene="rseP"
                     /locus_tag="O2692_002645"
     CDS             113..1399
                     /gene="rseP"
                     /locus_tag="O2692_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCZ4300232.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTEKKLTK
                     VSSETKYVLGFQPASEHTLFKPIVFGFKSFLIGSTYIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            1419..3122
                     /locus_tag="O2692_002646"
     CDS             1419..3122
                     /locus_tag="O2692_002646"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCZ4300233.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKVEVVYEPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQSLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
BASE COUNT         1123 a          438 c          647 g         1036 t
ORIGIN      
        1 attagacgtt atattttgtg atttgtcagt atcatattat aatgacttat gttacgtata
       61 cagcaatcat ttttaaaata aaagaaattt ataaacaatc gaggtgtagc gagtgagcta
      121 tttagttaca ataattgcat ttattattgt ttttggtgta ctagtaactg ttcatgaata
      181 tggccatatg ttttttgcga aaagagcagg cattatgtgt ccagaatttg cgatcggtat
      241 ggggccaaaa atttttagtt ttagaaaaaa tgaaacactt tacactatta ggttattgcc
      301 tgttggtgga tatgttcgta tggcaggaga tggcttagaa gagccaccag tcgagcccgg
      361 tatgaacgtt aaaattaaac ttaatgaaga aaatgaaata acacatatca tattagatga
      421 tcatcataag tttcaacaaa ttgaagcgat cgaagttaaa aaatgtgatt ttaaggatga
      481 cttattcata gaaggtatca ctgcttatga taatgaaaga catcatttta aaattgctag
      541 aaagtctttc tttgttgaaa atggtagctt agttcaaatt gctccgagag acagacaatt
      601 tgcacataaa aagccatggc cgaaattttt aacattattt gcgggaccgt tatttaactt
      661 tatattagct ttagtcctat ttattggtct tgcatattat caaggcacgc ctacgtctac
      721 tgtagaacaa gtcgcagata agtatccagc tcaacaagca ggattacaaa aaggtgataa
      781 gatcgtccaa attggcaaat ataaaatatc tgaatttgat gatgttgata aggcgttaga
      841 taaagttaaa gataataaga cgactgttaa atttgaacgt gatggtaaaa caaagtcagt
      901 tgaattaaca cctaaaaaga ctgaaaaaaa actgactaaa gtaagttcag agacgaagta
      961 tgttctcgga ttccaaccag cgagtgaaca tacacttttt aaaccaattg tattcggatt
     1021 taaaagcttt ttaatcggta gtacttatat ttttacagct gtagtaggta tgttggctag
     1081 tatatttacg ggcggattct catttgatat gttaaatggt ccggttggta tttatcataa
     1141 cgtcgactca gttgttaaag cgggtatcat tagcttaatt ggttacactg cgttattaag
     1201 tgtaaactta ggtattatga atttaattcc tattcctgca ctagacggtg gtcgtatttt
     1261 atttgttata tatgaagcga ttttcagaaa accagttaat aaaaaagcgg aaacaacgat
     1321 tattgctatt ggtgccattt tcatggtcgt tataatgata ttagtaacgt ggaatgatat
     1381 tcgacgatat ttcttataat ttaggaggat aaataattat gaagcaatcc aaagttttta
     1441 taccaacgat gcgtgacgtg ccatcagaag cagaagcaca aagtcatcgt ttattattga
     1501 aatcgggttt gataaaacaa agtacaagtg ggatttatag ttatttaccg ctagcaacac
     1561 gtgtgttaaa taatattact gcaattgtgc gacaagaaat ggaacgtatc gattctgttg
     1621 aaattttaat gccagcgtta caacaagctg aattatggga agaatcagga cgttggggtg
     1681 catatggccc agaattaatg cgtttacaag atagacatgg aagacaattt gcattaggtc
     1741 caacacatga agaattagtt acatcaatag taagaaatga attgaaatca tacaaacaat
     1801 taccgatgac attattccaa attcaatcta aattccgtga tgaaaagaga ccacgttttg
     1861 gtttacttcg tgggcgtgaa tttattatga aagatgcata ttcattccat gctgacgagg
     1921 catcattaga tcaaacgtat caagatatgt atcaagcgta tagccgtatt tttgagagag
     1981 ttggcattaa cgcaagacca gtagttgcag attcaggtgc tataggcggt agccatacac
     2041 atgaatttat ggcattaagt gctatcggtg aggatacaat cgtttacagt aaagaaagtg
     2101 attatgctgc taacatcgaa aaagtagaag tcgtttacga accaaatcat aagcatacta
     2161 ctgtgcaacc tttagaaaaa attgaaacac caaatgttaa gactgcgcaa gaattggcag
     2221 acttcttagg tagaccagta gatgaaatcg ttaaaacgat gattttcaaa gttgatggcg
     2281 aatatattat ggttttagtg cgtggccatc atgaaattaa tgacattaaa ttaaaatctt
     2341 atttcggcac agataatatt gaattagcaa cacaagacga aattgttaat ttagttggtg
     2401 caaatcctgg ttcactaggt cctgtaattg ataaagaaat caaaatttat gcagataatt
     2461 ttgtgcaaga tttaaataat ttagttgtcg gtgctaacga agatggttat cacttaatta
     2521 atgtaaatgt aggtagagac ttcaacgttg atgaatatgg cgatttccgt tttattttag
     2581 aaggcgaaaa gttaagtgat ggttcaggcg ttgcacattt tgctgaaggt attgaagttg
     2641 gtcaagtatt caaattgggt actaagtatt cagaatcaat gaatgctaca ttcttagata
     2701 accaaggaaa agctcaatct ttaattatgg gttgttacgg aattggaatt tctagaacgc
     2761 taagtgcgat tgttgaacaa aatcacgatg ataatggaat tgtttggcct aaatcagtta
     2821 ctccgtttga tttacattta atttctatta atcctaagaa agatgatcaa cgagaactag
     2881 cagatgcact atatgctgaa tttaatacta aatttgatgt gttgtacgat gatcgtcagg
     2941 aacgtgcagg tgttaaattt aatgatgccg atttaattgg tttaccactg cgaattgttg
     3001 ttggtaaacg tgcatcggaa ggtattgtag aagttaaaga acgtttaaca ggtgatagcg
     3061 aagaagttca cattgatgac ttaatgactg tcattacaaa taaatatgat aacttaaaat
     3121 aattaagatc gaatgaatta taagagtagg aaaaagctga aagaaatctg atgcttatgt
     3181 cctgctctta ttatttttga tataatgatt attcgatgaa aaatgactga agacatagta
     3241 taat
//
LOCUS       DALAGF010000161         3218 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.167, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000161 DALAGF010000000
VERSION     DALAGF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.167"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            92..>3218
                     /locus_tag="O2692_002647"
     CDS             92..>3218
                     /locus_tag="O2692_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin"
                     /protein_id="HCZ4300234.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENNSTNPIKKETNTDSQP
                     EAKKESTSSSTQKQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNN
                     TNNDVTTKPSTSEPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPV
                     NVSKEELKNNPEKLKELVRNDSNTDHSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVA
                     SNNVNDLIKVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKN
                     VIPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSY
                     IDKKTVPNETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQTIEQ
                     QIYVNPLKKSATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNSDQQLPQS
                     NRIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQ
                     GTSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQ
                     GTDSKEKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPTGYLPT
                     KVNGTTDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDAN
                     EPGIKDVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTT
                     ADDKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKD
                     VTVTLQNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDA
                     DGGEVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSD"
BASE COUNT         1319 a          505 c          577 g          817 t
ORIGIN      
        1 ttatctaaaa atgtattacg atgggaatac aaatttttat atgtaaaaat attaattttt
       61 tgcaaatatt gatattttaa aggagattta tatgattaac agggataata aaaaggcaat
      121 aacaaaaaag ggtatgattt caaatcgctt aaacaaattt tcgattagaa agtatactgt
      181 aggaactgca tcgattttag taggtacgac attgattttt ggtctaggga accaagaagc
      241 taaagctgct gaaaacacta gtacagaaaa tgcaaaacaa gatgatgcaa cgactagtga
      301 taataaagaa gtagtgtcgg aaactgaaaa taattcgaca acagaaaata attcaacaaa
      361 tccaattaag aaagaaacaa atactgattc acaaccagaa gctaaaaaag aatcaacttc
      421 atcaagtact caaaaacagc aaaataacgt tacagctaca actgaaacta agcctcaaaa
      481 cattgaaaaa gaaaatgtta aaccttcaac tgataaaact gcgacagaag atacatctgt
      541 tattttagaa gagaagaaag caccaaataa tacaaataac gatgtaacta caaaaccatc
      601 tacaagtgaa ccatctacaa gtgaaattca aacaaaacca actacacctc aagaatctac
      661 aaatattgaa aattcacaac cgcaaccaac gccttcaaaa gtagacaatc aagttacaga
      721 tgcaactaat ccaaaagaac cagtaaatgt gtcaaaagaa gaacttaaaa ataatcctga
      781 gaaattaaaa gaattggtta gaaatgatag caatacagat cattcaacta aaccagttgc
      841 tacagctcca acaagtgttg caccaaaacg tgtaaacgca aaaatgcgct ttgcagttgc
      901 acaaccagca gcagttgctt caaacaatgt aaatgattta attaaagtga cgaagcaaac
      961 aatcaaagtt ggcgatggta aagataatgt ggcagcagcg catgacggta aagatattga
     1021 atatgataca gagtttacaa ttgacaataa agtcaaaaaa ggcgatacaa tgacgattaa
     1081 ttatgataag aatgtaattc cttcggattt aacagataaa aatgatccta tcgatattac
     1141 tgatccatca ggagaggtca ttgctaaagg aacatttgat aaagcaacta agcaaatcac
     1201 atatacattt acagactatg tagataaata tgaagatata aaatcacgct taactctata
     1261 ttcgtatatt gataaaaaaa cagttccaaa tgagacaagt ttgaatttaa catttgctac
     1321 agcaggtaaa gaaacaagcc aaaatgtcac tgttgattat caagatccaa tggtccatgg
     1381 tgattcaaac attcaatcta tctttacaaa attagatgaa gataagcaaa ctattgaaca
     1441 acaaatttat gttaacccat tgaaaaaatc agcaaccaac actaaagttg atatagctgg
     1501 tagtcaagta gatgattatg gaaatattaa actaggaaat ggtagcacca ttattgacca
     1561 aaatacagaa ataaaggttt ataaagttaa ctctgatcaa caattgcctc aaagtaatag
     1621 aatctatgat tttagtcaat acgaagatgt aacaagtcaa tttgataata aaaaatcatt
     1681 tagtaataat gtagcaacat tggattttgg tgatattaat tcagcctata ttatcaaagt
     1741 tgttagtaaa tatacaccta catcagatgg cgaactagat attgcccaag gtactagtat
     1801 gagaacaact gataaatatg gttattataa ttatgcagga tattcaaact tcatcgtaac
     1861 ttctaatgac actggcggtg gcgacggtac tgttaaacct gaagaaaagt tatacaaaat
     1921 tggtgactat gtatgggaag acgttgataa agacggtgtt caaggtacag attcaaaaga
     1981 aaaaccaatg gcaaacgttt tagttacatt aacttacccg gacggtacta caaaatcagt
     2041 aagaacagat gctaatggtc attatgaatt cggtggtttg aaagacggag aaacttatac
     2101 agttaaattc gaaacgccaa ctggatatct tccaacaaaa gtaaatggaa caactgatgg
     2161 tgaaaaagac tcaaatggta gttcggttac tgttaaaatt aatggtaaag atgatatgtc
     2221 tttagatact ggtttttaca aagaacctaa atacaactta ggtgactatg tatgggaaga
     2281 tactaataaa gatggtatcc aagatgcaaa tgagccagga atcaaagatg ttaaggttac
     2341 attaaaagat agtactggaa aagttattgg tacaactact actgatgcct cgggtaaata
     2401 taaatttaca gatttagata atggtaacta tacagtagaa tttgaaacac cagcaggtta
     2461 cacgccaacg gttaaaaata ctacagctga tgataaagat tctaatggtt taacaacaac
     2521 aggtgtcatt aaagatgcag ataatatgac attagacagt ggtttctata aaacaccaaa
     2581 atacagttta ggtgattatg tttggtacga cagtaataaa gacggcaaac aagattcaac
     2641 tgaaaaaggt atcaaagatg tgacagttac attgcaaaac gaaaaaggcg aagtaattgg
     2701 aacaactaaa acagatgaaa atggtaaata tcgtttcgat aatttagata gcggtaaata
     2761 caaagttatt tttgaaaagc ctgctggctt aacacaaaca gttacaaata caactgaaga
     2821 tgataaagat gcagatggtg gcgaagttga cgtaacaatt acggatcatg atgatttcac
     2881 acttgataac ggatacttcg aagaagatac atcagacagc gattcagact cagatagtga
     2941 ctcagacagc gactcagact cagacagcga ctcagactca gacagtgatt cagattcaga
     3001 cagcgactca gattcagata gcgactcaga ttcggacagc gattcagact cagatagcga
     3061 ctcagattca gatagcgatt cggactcaga tagcgactca gattcagata gtgattcaga
     3121 ctcagatagc gactcagatt cagacagcga ctcagattcg gatagcgact cagattcaga
     3181 cagcgactca gactcggata gtgattcaga ctcagata
//
LOCUS       DALAGF010000162         3214 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.168, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000162 DALAGF010000000
VERSION     DALAGF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.168"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            389..>3214
                     /gene="sdrC"
                     /locus_tag="O2692_002648"
     CDS             389..>3214
                     /gene="sdrC"
                     /locus_tag="O2692_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrC"
                     /protein_id="HCZ4300235.1"
                     /translation="MNNKKTATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNETTAPSENKTTKKVDSRQLKDNTQTATADQPKVTMSDSA
                     TVKETSSNMQSPQNATANQSTTKTSNVTTNDKSSTTYSNETDKSNLTQAKDVSTTPKT
                     TTIKPRTLNRMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHVNQTTGKTEFWATSSD
                     VLKLKANYTIDDSVKEGDIFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDST
                     TNTTTYTFTNYVDQYTNVRGSFEQVAFAKRKNATTDKTAYKMEVTLGNDTYSEEIIVD
                     YGNKKAQPLISSTNYINNEDLSRNMTAYVNQPKNTYTKQTFVTNLTGYKFNPNAKNFK
                     IYEVTDQNQFVDSFTPDTSKLKDVTDQFDVIYSNDNKTATVDLMKGQTSSNKQYIIQQ
                     VAYPDNSSTDNGKIDYTLDTDKTKYSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTN
                     KDGKQDANEKGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGY
                     TPTTANVGTDDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDNDSDSDSDSDSDAGKHTPAKPMSTVKDQHKTAKALPETG
                     SENNNSNNGTLFGGLFAALGSLLLFGRR"
BASE COUNT         1291 a          509 c          560 g          854 t
ORIGIN      
        1 aaaaaacaaa ataaataata tgattaactt aaccaggtcc atgtggcctg gttttttctg
       61 tttagaaatt cccagatata aaacaaaaaa tctttaagga atagatacaa tttaatttgt
      121 tacagaaatg taattgtcta tgaattagaa aagctaagat attaatttca agggcaaata
      181 atactatttt ttatgatgat taaataaaga ttacaaaact cataaaaact tttatttaaa
      241 cacaaatgtt aaataacgtt ttacgataaa gaaaaataat taaagtattg cactttattg
      301 aaatttatat tacgatagta atgcagaaat ttatatatgc aaaatattat atttatcaaa
      361 ttttgatatt ttaaaggagt attattaaat gaataataaa aagacagcaa caaatagaaa
      421 aggcatgata ccaaatcgat taaacaaatt ttcgataaga aagtattctg taggtactgc
      481 ttcaatttta gtagggacaa cattgatttt tgggttaagt ggtcatgaag ctaaagcggc
      541 agaacatacg aatggagaat taaatcaatc aaaaaatgaa acgacagccc caagtgagaa
      601 taaaacaact aaaaaagttg atagtcgtca actaaaagac aatacgcaaa ctgcaactgc
      661 agatcagcct aaagtgacaa tgagtgatag tgcaacagtt aaagaaacta gtagtaacat
      721 gcaatcacca caaaacgcta cagctaatca atctactaca aaaactagca atgtaacaac
      781 aaatgataaa tcatcaacta catatagtaa tgaaactgat aaaagtaatt taacacaagc
      841 aaaagatgtt tcaactacac ctaaaacaac gactattaaa ccaagaactt taaatcgcat
      901 ggcagtgaat actgttgcag ctccacaaca aggaacaaat gttaatgata aagtacattt
      961 ttcaaatatt gacattgcga ttgataaagg acatgttaat cagactactg gtaaaactga
     1021 attttgggca acttcaagtg atgttttaaa attaaaagca aattacacaa tcgatgattc
     1081 tgttaaagag ggcgatatat ttacttttaa atatggtcaa tatttccgtc caggatcagt
     1141 aagattacct tcacaaactc aaaatttata taatgcccaa ggtaatatta ttgcaaaagg
     1201 tatttatgat agtacaacaa acacaacaac atatactttt acgaactatg tagatcaata
     1261 tacaaatgtt agaggtagct ttgaacaagt tgcatttgcg aaacgtaaaa atgcaacaac
     1321 tgataaaaca gcttataaaa tggaagtaac tttaggtaat gatacatata gcgaagaaat
     1381 cattgtcgat tatggtaata aaaaagcaca accgcttatt tcaagtacaa actatattaa
     1441 caatgaagat ttatcgcgta atatgactgc atatgtaaat caacctaaaa atacatatac
     1501 taaacaaacg tttgttacta atttaactgg atataaattt aatccaaatg caaaaaactt
     1561 caaaatttac gaagtgacag atcaaaatca atttgtggat agtttcaccc ctgatacttc
     1621 aaaacttaaa gatgttactg atcaattcga tgttatttat agtaatgata ataaaacagc
     1681 tacagtcgat ttaatgaaag gccaaacaag cagcaataaa caatacatca ttcaacaagt
     1741 tgcttatcca gataatagtt caacagataa tggaaaaatt gattatactt tagacactga
     1801 caaaactaaa tatagttggt caaatagtta ttcaaatgtg aatggctcat caactgctaa
     1861 tggcgaccaa aagaaatata atctaggtga ctatgtatgg gaagatacaa ataaagatgg
     1921 taaacaagat gccaatgaaa aagggattaa aggtgtttat gtcattctta aagatagtaa
     1981 cggtaaagaa ttagatcgta cgacaacaga tgaaaatggt aaatatcagt tcactggttt
     2041 aagcaatgga acttatagtg tagagttttc aacaccagcc ggttatacac cgacaactgc
     2101 aaatgtaggt acagatgatg ctgtagattc tgatggacta actacaacag gtgtcattaa
     2161 agacgctgac aacatgacat tagatagtgg attctacaaa acaccaaaat atagtttagg
     2221 tgattatgtt tggtacgaca gtaataaaga tggtaaacaa gattcgactg aaaaaggaat
     2281 taaaggtgtt aaagttactt tgcaaaacga aaaaggcgaa gtaattggta caactgaaac
     2341 agatgaaaat ggtaaatacc gctttgataa tttagatagt ggtaaataca aagttatctt
     2401 tgaaaaacct gctggcttaa ctcaaacagg tacaaataca actgaagatg ataaagatgc
     2461 cgatggtggc gaagttgatg taacaattac ggatcatgat gatttcacac ttgataatgg
     2521 ctactacgaa gaagaaacat cagatagcga ctcagattct gacagcgatt cagactcaga
     2581 tagcgactca gattcagata gcgactcaga ttcagacagc gattcagaca gcgactcaga
     2641 ctcagatagc gattcagatt cagacagcga ctcagactca gacagcgatt cagactcgga
     2701 tagcgactca gactcagata gcgactcaga ttcggatagc gactcagact cagatagcga
     2761 ttcagattca gatagcgatt cggactcaga cagtgattca gattcagact cagatagcga
     2821 ctcagattct gacagcgatt cagactcaga cagcgactca gactcagaca gtgattcaga
     2881 ttcagacagc gactcagatt cagatagcga ctcagactca gatagcgact cagactcaga
     2941 tagcgactca gactcggata gcgattcaga ttcagacagc gactcagatt cagatagcga
     3001 ttcggactca gacaacgact cagattcaga tagcgattca gattcagatg caggtaaaca
     3061 tactccggct aaaccaatga gtacggttaa agatcagcat aaaacagcta aagcattacc
     3121 agaaacaggt agtgaaaata ataattcaaa taatggcaca ttattcggtg gattattcgc
     3181 ggcattagga tcattattgt tattcggtcg tcgt
//
LOCUS       DALAGF010000163         3177 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.169, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000163 DALAGF010000000
VERSION     DALAGF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.169"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(386..2995)
                     /gene="adhE"
                     /locus_tag="O2692_002649"
                     /gene_synonym="adhC"
     CDS             complement(386..2995)
                     /gene="adhE"
                     /locus_tag="O2692_002649"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCZ4300236.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
BASE COUNT          915 a          586 c          483 g         1193 t
ORIGIN      
        1 aatacttctt taatttttgt taattctaat gtactttcca ttatttacct cccttaaaaa
       61 ttttcattaa aattggtatt atatatatag tatttacata ttacatatcg tttgaacaat
      121 taattacttt taaaccgcat tattttcgcc tttttattta tatgaaatta aaaataatta
      181 tactctaaag tagtaatagt ttgtgaaatg agtatcaatc ttaatatttt ctttatttaa
      241 aaaaatgatt gtctagtttg tatctctctg aagatttggc aataaataaa agccgataac
      301 cgtataatga ttatcgactt aaagtttatg tggcattttt tacttttgta atttcaggtg
      361 agttagatga ttattatcag atagattatt gcttataatc atatgatgtt tgaatgatat
      421 ctttgatttc actgattagt gcttctttag gattagcagt tgtacattga tcttcaaatg
      481 cgagctctgc cattctatca attgactcat ttaattcttc ttcagacaca ccttgtgatt
      541 tcaaattcat ttcaattccg actgattgac ctaattcgta gacagcttta gctaatgatt
      601 ctacgagtgc ttctgtcgta ttacctttta atcctaagaa tttggcaata tctgcataat
      661 ctgtatctgc tctgaagaac tcatatttag ggaataatgc atgtttttgc gggtctttgg
      721 cattataacg gataatatgc ggtagtaata tcgcattcgc tctaccatgc ggaataccat
      781 attcgccacc aattttatgc gcaattgagt gtgcaatgcc taagaatgca tttgcaaatg
      841 ccataccagc caaagttgat gcgttatgca ttttctctct tgaaacttta tcaccctttt
      901 caacagatga ttttaaatat tcgaacgtca atttaatcgc ttgtagactc aaacctcttg
      961 tgtagtctga agccattaca gatacatatg attccattgc atgcgttagt acatccattc
     1021 ctgtatctgc tgtaacgctt tttggcacac tcatcacaaa ttgagggtca ataattgcaa
     1081 cgtcaggtgt taaagcaaaa tcagccaacg gatattttac atttgtttca ctatctgtga
     1141 taactgcaaa tggtgttact tctgaacctg tacctgatgt cgtagggata caaatgaacg
     1201 tcgcattttc aggcatgcct attttataag tacgtttacc gatgtctagg aacttttgtt
     1261 tagcaccgaa gaatgatgtc tcagggtgtt caaagaacat ccacattgct tttgcagcat
     1321 ccatcgctga accaccacca agtgcaatga ttgtatccgg ttggaaatca accatcattt
     1381 ccagaccttt atatactgta ttagttgatg ggttcggttc gacttcgcta aatattttaa
     1441 tttgaggctg ttccgttttt tgtcttaata cattctcaac tgtttttgta taaccgaatt
     1501 ctaccatacc agggtcacag acaatcatca ctttttcaat cttgtccatt gttgttagac
     1561 tcatgattgc attttcttca aaataaattt gagcaggcac cttgaaaatt tgagtattat
     1621 tacgtcgttt agcaatcgtt ttaatgttta ataaatctgt cgcactaaca ttatgtgaaa
     1681 ttgagtttct accgtaggaa ccacaaccta atgttaaaga cggaatcaat tcgttataca
     1741 tatcaccaat acctccaacc gctgatggtg tatttacaag tacacgacaa gctttcattc
     1801 ttagtccaaa atctttttgt aatgtttcat cttctgtatg gataacggct gtgtgtccta
     1861 atccaccaaa atgtagtgtg tcttcacaaa tttgaaatgc ttgttttgta gattgggctt
     1921 ttactaaggc taatactgga gataattttt cacgagataa cggatagtct gaacctacac
     1981 cgctaatttc ggctatgata agttttgtat tttcggggac aggtatacct gctaattcag
     2041 ctatttcaac tgcagattta ccgacaatat caggcttaat acctgttttt tgttcattca
     2101 taattgcatt ttctaagcgt tgtaattcat cttttttaac aaagtatgct tgatgtgctt
     2161 taaattcatt agtaacatct ttataaattt ctttatcaat gactacaact tgttcagaag
     2221 cacaaatcat accattatca aatgtttttg aaccaatgat atcatttact gcacgtttaa
     2281 tgtgtgctgt tttttcaatg taagacggca cgttacctgg tcccacacct aatgccggtt
     2341 tgccagttga atatgcagac ttaaccatgc ccgaaccacc tgttgctaga actaatgcaa
     2401 tacctttgtg attcattaat tgttttgttg cttcgataga aggcacttca atccactgaa
     2461 taatatcttt aggtgcacct gccttcattg ccgcttctaa tacaacttct gctgcacgct
     2521 tcgacgattc ttgtgcactt ggatggaatg caaaaatgat tggatttcct gtcttaattg
     2581 caatcatcgc tttaaaaata gttgtcgacg taggatttgt tgttggcgta acaccacaaa
     2641 taacaccaat tggttccgct acatacgtta atcctttttc tttatcttca ccaataatcc
     2701 ctactgtctt attgtctttt attgaattcc atatatattc agaagcgtat aaatttttaa
     2761 tcgctttatc ttcgtatata cctcttccag tttcttcatg tgctaatttt gctagcacca
     2821 tatgttgatc aacagctgct aagctcattt gatgaacaat atgatcaatt tcttcttgtg
     2881 actttttaga taatgcttct aatgcttttt tccctttgtc agctagagca tcaatcataa
     2941 ttgccacttc ttgttctttc gatccacgat tttctttttc aggtatagtt aacatataca
     3001 accactcctt tatactttgt gaattatttc acaaacatta tagtacatgt ctctcaggat
     3061 ataaagaaaa ttctatacaa aaaagtttaa tttcgaatat tatttgaaca aatatcaaat
     3121 tttaaaataa atgttttcat gaaatcattg ttatttcggt gtttttagaa tgatttt
//
LOCUS       DALAGF010000164         3154 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.170, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000164 DALAGF010000000
VERSION     DALAGF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.170"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(40..732)
                     /locus_tag="O2692_002650"
     CDS             complement(40..732)
                     /locus_tag="O2692_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HCZ4300237.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            1117..1914
                     /locus_tag="O2692_002651"
     CDS             1117..1914
                     /locus_tag="O2692_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCZ4300238.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            2047..2994
                     /locus_tag="O2692_002652"
     CDS             2047..2994
                     /locus_tag="O2692_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCZ4300239.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
BASE COUNT         1124 a          417 c          587 g         1026 t
ORIGIN      
        1 aagatatttt ctaattgaaa atcatcttac tgctgttttt taggaatcga tggggtttaa
       61 aaattgatga tagttatcaa tggatatgtc tctggtttga atcgtcttac cgaatgaata
      121 cgaaatatct ataatatttt caatttcgag ttcggcagtt ttgtcttgag caatgtcata
      181 aatgactgca tgccaatgtc catgtttaca caaaaggcca ataaatataa cgttttcagc
      241 tttaatatta tcatcaaccc tatgatctac taacatcaca tttcgttctg tacaataaat
      301 taaaatgtcg gaaaacagca ttgggagtgt catatgttgt gtgtcatcaa atttaatata
      361 aaagtccatg cgtttaagca attttcttaa gcgtgtttgt ggaagactta aacattgttt
      421 tataattgcg ttgatttcgc ttttatatgg taatgtcgag tacgactgac tttcattaag
      481 cactaaaaat aaggctgata attgtgtttc agataagttt aatgcaattt tagattgtgc
      541 ttgttgtatt tggtaaccac catttttccc ataatgcgca taaattttaa caccttgatt
      601 ttctaaatca tcaatatctc ttaaaattgt gcgtttggat acgttgcaat atttagctaa
      661 ttctaaagcg gtcatttttt tattttgttg aattgcagta attattaaat tttgcctttc
      721 agctttattc atgacagcca cctcctaaaa gatgatatat tacacaaagt ataacataag
      781 agtgacaatg aggacatttt caatatcttt taaaaaaaat cgtaaaaatt ttgtggaaaa
      841 cgcttacatt ttaaaaaaga ttgtgctatg atattacttg taacaggggg agcgattaaa
      901 caaaggggta gagctatata acagatagct tttaatcgtt caagttacat cacaacatct
      961 tcaatattta ttacttactt tcctttctat ttgtcggcta gcacgtgact agccgatttt
     1021 tttatgcata aaatgaagaa gtcttcagtt gtattgttga ttcttttaat atagggtaat
     1081 aataaaatga taatgatatg agaagtggtg gacgaaatga cagataaaac attacagcaa
     1141 atagataagt taatatgttc gtggttgaaa caaatagaca atgttattcc gcaattaatt
     1201 atggaaatga ctacagaaac taagcgtcat cgatttgatt tagttacaaa tgtagataaa
     1261 cagattcaac aacaattcca acaattttta gcaacacatt ttccggaaca ccaactatta
     1321 gcggaagaaa aaagtaatga aatgattacg aatgaaatta atcatttatg gattatggat
     1381 cccattgatg gaactgccaa tttagtaaaa cagcaagaag attattgtat tatattggcg
     1441 tatttttatg aaggtaaacc aatgttatcg tacgtatatg attatccgca taaaaagctt
     1501 tataaagcaa tacgaggaga aggtgctttt tgtaatggaa ttaagatgga agaaccacca
     1561 tcgttaaaat tggaagacgc tattatatcg tttaatgcac aagtgatgaa tctagatacg
     1621 gtgcaagatt tatttgacgc ctcatttagt tatcgtttag ttggtgcttg cggattagat
     1681 tctatgcgag tggcaaaagg tcaatttggg gcacatatta atacaaatcc taaaccttgg
     1741 gacattgctg cacagttttt atttgcagaa ctattgaatc taaaaatgac gactttagat
     1801 ggtaaggcaa ttgatcattt aaagggtgca ccttttatta taagtaataa agcatgtcat
     1861 gaaacggtac ttaaaatttt aaatgcaaat ggtggttatc aaaaatacag atagatattc
     1921 tctagagata aaaaatgata aaaaatagtt atacgtctac agaagtatag taaaaaaatt
     1981 attttactat ataataaata gagaaaacag acaaagtatg taaggaatag taaaggagtg
     2041 gtgggattga aacgttcgtc taaaagtaaa atgagcctac cgaaaaaaat atttttatgg
     2101 gtattcggta ttttagtaat cttagcgatt gtagctgttg tgtatgtagc tgctaaaatt
     2161 tttattactg gtaataagat acacaatccg ttagatcgca atcattcaga attaagagat
     2221 aaaaaagtga gtttaaacga tggtgatcca ttcacaatcg ctttatttgg tgtcgattca
     2281 gatgctgatc gtaagaaaaa gggtggcgga gagcgtagtg atagtattat gattttatct
     2341 atcaacccta aaacgaagaa aactgaaata gtaagtatac cgcgtgatac aagagcggaa
     2401 attgttggcc gtggtactac tgaaaaaatt gcacatgcct atgcatatgg tggaccaaat
     2461 atggctgtta aatcacttga aaaattaatg aatgtgccaa ttgatcatta tgcgacaatc
     2521 gatatggatg gtttgcataa tatgatagat agcatcggcg gtgttgatgt agtaagtaat
     2581 gatacattca ctgttgatgg tgttcgtttt acaaaaggac agcagacaca tgtaaatggt
     2641 gatcaagcat tgaagtttat tagaagtcgt aaagaagaag gtgctggtgg tgactttggt
     2701 cgacaacaac gtcagcaaat tgtgcttgaa gcgatggcca ataagattgc aagcccatca
     2761 tcgattacac attttaatag tctaatgaat gaaattcaaa ataatgttaa aacagattta
     2821 acattaggtg atttgaatac gattagaagc aattataaag atgctaatga cacgattaat
     2881 aaacatcaat tgagtggtca aggcggcatt caaagtgacg gattgtacta cttcattcct
     2941 agtgagcagt caaaagctga aagtactaag ttattaaaag ataatctaga gtagtaaact
     3001 aagggaaaat aagttatcaa aattgtacac aaattaatta gatagaatga tctgtaaata
     3061 gaataacgaa tagaactaat agcccgtaaa tagaaggtga taacactttg tatttatggg
     3121 ctattttatt taatgcctga tttgattttt ataa
//
LOCUS       DALAGF010000165         3125 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.171, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000165 DALAGF010000000
VERSION     DALAGF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.171"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(55..243)
                     /locus_tag="O2692_002653"
     CDS             complement(55..243)
                     /locus_tag="O2692_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300240.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILM
                     GINAIYLVVKVMNFKKNN"
     gene            complement(826..1821)
                     /locus_tag="O2692_002654"
     CDS             complement(826..1821)
                     /locus_tag="O2692_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCZ4300241.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADIL
                     EILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQK
                     VIEKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFM
                     RSTEVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVAN
                     VKAIKPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSY
                     GGLLKQKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     gene            2273..2854
                     /locus_tag="O2692_002655"
     CDS             2273..2854
                     /locus_tag="O2692_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300242.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIA
                     DKNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDI
                     ENKKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVA
                     KKLAKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
BASE COUNT         1139 a          519 c          379 g         1088 t
ORIGIN      
        1 aaaaaaacag tgtgaaaaca agaagaaact cgtttcaaaa tcccctcaaa tattctaatt
       61 attctttttg aaattcataa ccttaacaac caaataaata gcatttattc ccatcaaaat
      121 aacatacgga attaatatat ataaattttc caaatgagat ataaaaatta atccaagtaa
      181 aaagaaaaca tttaaaatag ttgctataac gtaaccttca tctaacactg tgtttttatt
      241 caaaataaac actccttttt taaaaaactt tatcacaaga cttactcaaa ttataaatac
      301 ctttattttg agttttaagg gggtatttta aaggtttagg gggtatcata taggttttta
      361 acttaaaaat atatacaagc tcttaaaacg caaatatgag ccaaataaat atatggtaat
      421 ttcacaaaca aaaatttcag caaaaccagt gactaatttt ttaacactgc ccatttacat
      481 gcaaattaaa aattagcata aaatccttta aaacttccac atgtttttct aaattcctgc
      541 agggcgtttt ttatcaaaaa tcgagaattt ttaatttttc tctaaaaact ccgtaagagc
      601 ataaggagaa tttgctatca ttcaaaaatg ataggtctgg caaagccaga aattccaggg
      661 gttttaaggt attttaaagt gatatcatac aaagctgtac tgtcttattt ttgtgacaca
      721 atatattgcg tttgtcttat ttttgtgaca ctacatacag catttgtcac aaaaataaga
      781 cagtactttt ttataaaaaa tcaacaaaaa agacattagt caacactaat gtcttaaata
      841 ataattttgc ttttcgaaat tccaaattgc ggtaattgaa tgtgcttttt cttcttcgtc
      901 tgttgtttta tcttcatcac ttgcattaat caaattacca tcttcgacat cgtctaagtt
      961 taaaatttta tgtttttgtt taagcaatcc accataactt aacatacgtt ttcgatacaa
     1021 acctttttct aaatcactta caatttcttg atttttttca tcatcatcag ttaaaaaatc
     1081 agatgattta accgaatatt ttgaggtctc tttgattgcc gattcaatgt ctttatcgcc
     1141 ttttctattc ggtttgatcg ctttaacatt agcaacaggt cgatagtcaa cttgtaatgc
     1201 tctttgccat aaattaaccc attcttcttg agttatataa ttctcttttt ttctaaaata
     1261 tgcattttca acacataaca aaacatgcat gtgctgatta taactaccgt catttttatt
     1321 aacggtaact tctgttgaac gcataaatcc aacaagattt tgtttaacct ttttatatct
     1381 actcaaccta tcaaatgctt tagttagatg cttcaaactt tgttctaaag tatctccatc
     1441 tatcgcattt tttgttgaaa gtgttaaaaa caaccaacgt gcttttggct tttccttaat
     1501 tactttttca atcacttttt gagcttgata actatttttc atagcacgtc tccaattaca
     1561 aaccggacat aattttgatt tacaaaacca tgtcttatgt aacttcaaat agccttcatc
     1621 agttggctta aattctaaaa tattaccaca ttgttttaca ttaaaagcct ttttgatttt
     1681 taaaatttct agtatatctg catagcttac attatctatt ttcttttctc tccatgggcg
     1741 ttgtttccca cttttcgtca tatcttttaa cgttttatta tcttgatttt cggttatact
     1801 tctagtagta ttatattgca tataaaaaga cccccagtat tatttaagat tcgacacctt
     1861 aataatataa ctggggtttt ttattgtcaa attttccttt cacgcacaaa aacacgttta
     1921 aacactgtaa tatcaacagt tacagctata tatataaaat cgagttattt ctatatagta
     1981 tcaagacaag aagaaactcg ttcacactcg tttcaaaaaa caacctattc cttcttcttt
     2041 ttatgaccct ctaaacctaa ataaattaat gtgaaaacca atgaataaat aaaactttct
     2101 aaaaagtcaa aaggattatc aaataaaatc gaattaacaa aattaaaaat taaaaataca
     2161 gataataaaa taatcaaaaa gtaaaaaaac ttattcatat taaactcctt tgtttttata
     2221 tgttaatatt atattgaatt catttaaata acaaaacaag ggggcattta caatgaatgc
     2281 aattcaaaac agtatatcac taagaaagat tttagtagta tctttaagta ttttaaccat
     2341 actttcaatg gtcttggatt ttaatttcaa agaagctcaa gcccaaaaca aaaataacat
     2401 tgcagataaa aacgttgaaa ctttaaacga aaaagaaatt gaaaaagaac taaaatacat
     2461 atatggcaaa attattattt tagataaaga cggaacagct aaagatgtta atctagaaaa
     2521 tgccaaatct cgatatggat atgttcctga aggattccaa aaactaaaaa acgacataga
     2581 gaataaaaag aaaactagtt caatatcacc tagagcagtt ggtgggaact acaaaaatag
     2641 cggtgactgt ttctattctg aagttttaaa ctcatatgga gaacttttaa ctggaaacat
     2701 aatcacagca gtttttgaag atgttaaagc caaaaactta aagggcgtag caaaaaaatt
     2761 agctaaaatt gggataaaag gaaacctagc tggaatcgca gctactatgg tttcgaagga
     2821 tgtacaatgt aatcttaaat acgggttact ttagatacta tttattccaa ttgctttatt
     2881 gacgttgagc ctcggaaccc ttaacaatcc caaaacttgt cgaatggtcg gcttaatagc
     2941 tcacgctatg ccgacattcg tctgcaagtt tagttaaggg ttcttctcaa catcaataaa
     3001 ttttctcggc ataaatgcgt cgtctatttt ttattatctt catgataatc atatttttct
     3061 tccatctcgt caaaagcatc atctttatag aaaaacaaaa agtaagcaac aacaccaata
     3121 ggaat
//
LOCUS       DALAGF010000166         3029 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.172, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000166 DALAGF010000000
VERSION     DALAGF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.172"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            45..1151
                     /gene="rpoD"
                     /locus_tag="O2692_002656"
     CDS             45..1151
                     /gene="rpoD"
                     /locus_tag="O2692_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCZ4300243.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            1282..1959
                     /locus_tag="O2692_002657"
     CDS             1282..1959
                     /locus_tag="O2692_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCZ4300244.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     ELIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            1962..>3029
                     /locus_tag="O2692_002658"
     CDS             1962..>3029
                     /locus_tag="O2692_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCZ4300245.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKALLMNWFNTEKINLDVEAS"
BASE COUNT         1138 a          389 c          586 g          916 t
ORIGIN      
        1 gaataaattt cgaatgttca tatttaaaat cgggaggccg tttcatgtct gataacacag
       61 ttaaaattaa aaaacaaaca attgatccga cattaacatt agaagatgtt aagaagcaat
      121 taattgaaaa aggtaaaaaa gagggtcatt taagtcatga agaaattgct gaaaaacttc
      181 agaattttga tatcgactct gatcaaatgg atgatttctt tgatcaatta aatgataatg
      241 atatttcact agttaatgaa aaagatagtt cagatactga cgagaaactg aatccaagtg
      301 atcttagtgc ccctccaggt gttaaaataa atgacccagt tcgtatgtac cttaaagaaa
      361 ttgggcgtgt taacttatta agtgcacaag aagaaatcga attagccaaa cgtattgaac
      421 aaggtgatga agtagcaaaa tcaagacttg cagaagcgaa cttacgttta gttgtaagta
      481 ttgctaaaag atacgtaggt cgtggtatgt tattccttga tttaatccaa gaaggtaata
      541 tgggtcttat taaagctgtt gaaaaatttg actttaacaa aggatttaag ttttcaacat
      601 atgcaacatg gtggattaga caagcaatca ctcgtgcaat tgctgaccaa gcacgtacga
      661 ttcgtatccc tgtgcatatg gtagaaacaa ttaataaatt aattcgtgtt caacgtcaat
      721 tattacagga cttaggtcga gatccagcac cagaagaaat tggtgaagaa atggatttac
      781 cagcagaaaa agttcgtgaa attttaaaaa ttgcgcaaga acctgtttca ttagaaacac
      841 caattggtga agaagatgat agtcatttag gagactttat tgaggatcag gaagcacaaa
      901 gtccttcaga tcatgctgct tatgaattat taaaagagca attagaagat gtgcttgata
      961 cattaactga tagagaagaa aatgtattac gattaagatt tggtcttgat gacggcagaa
     1021 caagaacact tgaagaagtt ggtaaagttt tcggtgttac acgtgaacgt attcgacaaa
     1081 ttgaagcaaa agcacttaga aaattaagac atccaagtcg tagtaaacgt ttgaaagact
     1141 ttatggatta atttatatca attttaatga ttgataccaa gacatgaata agcgcttatt
     1201 tatttttaga ttaagattta acttgagtga ggttggtaca ttgaataatg ttcaacctct
     1261 tttttcataa aggagaataa aatgatttcg ttaaataacc gattaacgac ggtaagtcga
     1321 tttttaaaac aaggtacaat cgcggatatt ggctcagatc atgcttattt gccaatctat
     1381 gcaattcaaa accatttatg cgaatgcggt attgctggag aagttattca aggccctttt
     1441 caagctgctg tgaaaaatgt agctgcaaac caattagtag atagaattga tgtacgttta
     1501 ggggatggct taagtgttat tcaacctgag gacgtaatcg ataatatcac aatttgtggt
     1561 atgggcggac cacttattgc taaaatttta aaagatgggc aagataagtt aagtcaacat
     1621 ccaaggttga ttttacaaag taatatccaa actgaaaatt taagacaaac attacaacaa
     1681 ttgaattatg aaataattga tgaaataata atggaagaaa aaggccatat ttatgaaatt
     1741 gttgtagcag aatattctac agaattaata gagctatcat ctgatgaatt gaaattcgga
     1801 ccaaaattgc tgaacaacaa aaatgaatat tttataaaaa aatggcaaag agagttagaa
     1861 gcactttatc atattaaatc gaaactcaat actgaacaac atcatcaacg tttagctcaa
     1921 attaatgacg aaattgctgt aattgaaagg gtgttatagt aatgaaaata gctgatttaa
     1981 tgacattgtt agatcatcat gtgccattta gtactgctga atcttgggat aatgtaggat
     2041 tgttaatagg tgatgaagat gttgaagtta ctggtgtttt aacagcatta gactgtacgt
     2101 tggaagtagt aaatgaagca atcgaaaaag gttataatac tattattagt catcatcctc
     2161 taatctttaa aggcgtaaca tcattaaaag ctaatggtta tggtttgatc attagaaaac
     2221 taattcaaca tgacattaat ttaatagcga tgcatacaaa tttagatgta aatccgtatg
     2281 gtgtcaatat gatgttggcg aaggcgatgg gtttgaagaa catttcaata ataaataatc
     2341 aacaagatgt atactataaa gttcaaacat atatacctaa ggataatgtt ggaccattta
     2401 aagataagct tagtgaaaat ggattagcgc aagaaggtaa ttatgaatat tgtttctttg
     2461 aaagtgaagg aagagggcaa ttcaaaccag ttggtgaagc taatccaaca ataggacaaa
     2521 ttgataaaat tgaatatgta gatgaagtta aaattgaatt tatgatagat gcatatcaaa
     2581 agtcaagggc tgagcaatta attaaacaat accatccata tgaaacaccg gtatttgatt
     2641 ttattgagat aaaacaaaca tccctttatg gacttggcgt tatggcagaa gtggataatc
     2701 aaatgacatt ggaagatttc gcagctgata ttaaatctaa attaaatatc ccaagtgtcc
     2761 gttttgttgg tgagtctaat cagaaaatta aacgtattgc aattattggt ggttcaggta
     2821 ttggatatga atatcaagct gtccaacaag gcgcagatgt ctttgttaca ggtgatatta
     2881 aacatcatga tgccttagat gctaaaattc atggtgtgaa tttaattgat attaatcatt
     2941 acagcgaata tgtgatgaaa gaaggtttaa aagcgttact aatgaattgg ttcaatacag
     3001 aaaaaataaa tttagatgtt gaagcatct
//
LOCUS       DALAGF010000167         2922 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.173, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000167 DALAGF010000000
VERSION     DALAGF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.173"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(98..745)
                     /locus_tag="O2692_002659"
     CDS             complement(98..745)
                     /locus_tag="O2692_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCZ4300246.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQRAGMQSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            complement(858..1406)
                     /gene="hxlB"
                     /locus_tag="O2692_002660"
     CDS             complement(858..1406)
                     /gene="hxlB"
                     /locus_tag="O2692_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HCZ4300247.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            complement(1408..2040)
                     /gene="hxlA"
                     /locus_tag="O2692_002661"
     CDS             complement(1408..2040)
                     /gene="hxlA"
                     /locus_tag="O2692_002661"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HCZ4300248.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIIDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            complement(2117..2875)
                     /gene="nagB"
                     /locus_tag="O2692_002662"
     CDS             complement(2117..2875)
                     /gene="nagB"
                     /locus_tag="O2692_002662"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCZ4300249.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNEDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
BASE COUNT          875 a          566 c          414 g         1067 t
ORIGIN      
        1 tattttaaat tcctaatcta aagcgaaata tttctaagta taatctaata tttatgaata
       61 tgcattttaa ccatggtatt ctctgtttta acaagtttta taatacggta ttaatttctg
      121 atggatcatt aataataaaa tccggatttg aatgaagtaa tgaccttgca tcatgtgcac
      181 cccaagtgac agctgcagat tgcataccag cacgttgtgc catctcaaca tcaaacgttg
      241 aatcaccaat atacaccgtt tgttggctat ttaaattgta gcgttgcaca attgtgtgta
      301 tgccttcagg atttggttta tatgcactta cttgatcgga tccaacagct tcggtaatca
      361 agtgatttaa tccaatagcc gataaatttc tttctaatac atcactcttc ttactagaca
      421 ccacgaaaag ttttttccct tggttataca aacttgtaat ggcttcagtt atacccgcaa
      481 attcataaat atagtccttt tcaatagatt gatatgtatg tctaaatgta tcgattaact
      541 ttgctaatgc tgcttcatct aatggtcggt ctgctaattt taaaaatgat tcttcaatag
      601 gtattcccat ataatgcgtt atttctttag atgatggttc cgttaagcca catgctttaa
      661 atgcactttg tgttgctact tcaccacatt ttttcgtgtc tgccaacgta ccatcaaaat
      721 caaaaatata attgtcaaac ttcaatatta acacccctat tcgtttatta ttgatattat
      781 actataattt attatttaaa taaaaaaata atcgataata tttctgccta ggcatcatat
      841 tatcgactat ctttatttta ttctaaatta gcatgatttt gttgcatcgt ttgttccgta
      901 acattcattt cagtcatcaa tcccattaca acactatcta aaaataattg agatgcttgt
      961 tcaaacaaac ttcctaatgg ttgtgccgag ccttgttcat catattttgt acctgcaggc
     1021 aaaacgatgt tcgtcccagc tagattgcct attgcagaat ctttatttgt agtaattaat
     1081 acgatgtcag cacctactga ttttgctttg tctgctaata atcttaaatg ttccgtggaa
     1141 cctgaaccag agataattac aaatacatca ttcgacttaa tcgcaggtgt cgttgattct
     1201 ccaacaacat gtgcctgttt gccgagctga tttaagcgca ttgcaaaact attcgccacg
     1261 aatcctgaac gtcctttgcc agctacaaat atatgttcag catgtagtat tttggatgca
     1321 aaagttgaaa actcatccgc ttcaacatgt gacaaagtca tctttaattc atctagaatt
     1381 aattgatagt cactaaattt agccatatta cttaccttcg attgcagcgc gacattgttt
     1441 tgcagcttct actggatcat ctgcatttgc gattccgcca ccaacaataa caagatcagg
     1501 actttcagcg acaatatctt taattgtatc tggtttaatt ccacctgcta ctgcaacttt
     1561 agaattttta ataacagatt taacggttct taaactttct aatggtgatt gcccttctgc
     1621 ttgtaaatca taaccagtgt gtactgcaat ataatcagca cccatttcat ctagttcttt
     1681 tgcacgtttt tctaaatctt gaacagcaat catatcaact agtaattgtt tattattttt
     1741 atgagcttct tcaatagctg ctttaattga tgcatcttct gcaacaccta gtattgtaat
     1801 tacatccgcg ccaaatttaa ttgcttggct aacttcataa tcagctgcat ctataatttt
     1861 catgtctgct aatactttta cattactaat gttgtctgcc atatgtttaa ctgctggtaa
     1921 accttcgttg taaatgattg gcgtaccgat ttctacgata tctacataat cttttacttt
     1981 atttgctaac tcagccgcgt cttctttgtt taataaatca attgctaatt gtaattccac
     2041 taaaaatcat ccttccaatg ctttttatct ttctattgga acctttcccg aggcatttct
     2101 taattaaaca ttgcaatcaa tccatttcat cgacatgaat tttcgcaaca tttttcgggc
     2161 aagcttcgtc gtctaaatat atctcaacat tcgggtgttt gtgaagtaat gtggctggaa
     2221 catcaacaga aatttcctga tttaataaat gtgtaatagc agcacgtttc ttttcaccaa
     2281 atgcgagtaa aatgatacgt ttggcttgaa gaatattagc aagtcccatc gaaatggctt
     2341 gctttggaac atcatcttcg tttttaaaat atcgactatt agccttaata gtactttcag
     2401 tcaaatcaac gatatgagta acgctatcaa acggcgtacc aggttcatta aatccaatat
     2461 gaccattttc accaatacct aaaatttgaa tatcacgttg accttgttgt tctaaaacgt
     2521 cattatattt tgacgcttcc gcattcatat catcggcatc ttcatttgga atatgaatgt
     2581 tctttctatt aaaataagga tattgtttga aaagcatgtc atccatataa tagtgataac
     2641 tttgcggatg tgatgcggtt aaacctacat attcgtctaa attaaacgtg gatacattgt
     2701 ctacgtttaa ctgattttta tttaataact taacaagttg ttcatacaaa tctgtcattg
     2761 taccaccagt tgctaaacct agtttacagt gctgattaaa tgccatctct ttatataact
     2821 cacatgcaac atagaatgat gcttgttttt tcgatcctaa gtttaatact ttcattgttt
     2881 acatgcctcc acaaatttaa tacattttaa aaacttatga at
//
LOCUS       DALAGF010000168         2858 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.174, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000168 DALAGF010000000
VERSION     DALAGF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.174"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            233..1168
                     /gene="lukE"
                     /locus_tag="O2692_002663"
     CDS             233..1168
                     /gene="lukE"
                     /locus_tag="O2692_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HCZ4300250.1"
                     /translation="MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKR
                     TEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRM
                     IWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFN
                     YSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGS
                     AREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRT
                     GIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
     gene            1170..2153
                     /gene="lukD"
                     /locus_tag="O2692_002664"
     CDS             1170..2153
                     /gene="lukD"
                     /locus_tag="O2692_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HCZ4300251.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            2395..2538
                     /locus_tag="O2692_002665"
     CDS             2395..2538
                     /locus_tag="O2692_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HCZ4300252.1"
                     /translation="MEKVLDLDVQVKGNNNTNDSAGDERITSHLFCSFGCEKTGSFNS
                     FCC"
BASE COUNT         1093 a          381 c          489 g          895 t
ORIGIN      
        1 aaagattgag tatcggtaat tttatcatat tgttcgaata taaagggaac tatttaaagg
       61 taatgaaggt tgcgccaaaa tgaccggttt tcagcagcat aaatgaataa attgtgtcat
      121 ttttaatatt ttatagcatt ttatttttta atgaacagtt aattttataa ataagtgaaa
      181 taatctagaa taaatttgta ttaaaaattt atatagaaag aaagtgaaac ttatgtttaa
      241 gaaaaaaatg ttagctgcaa ctttgtcagt aggactgatt gcacctttag catctccgat
      301 tcaagaatct agagcaaata ctaatattga aaatattggt gatggtgctg aagtaatcaa
      361 acgtacggag gatgtaagta gtaagaaatg gggcgttact caaaatgtcc aattcgactt
      421 tgtaaaagat aaaaaatata acaaagacgc tttaattgtt aaaatgcaag gttttattaa
      481 ttccagaact tcattttcag atgtgaaggg tagtggatat gaattaacta aacgaatgat
      541 ttggccattc caatataata taggactgac gactaaagat ccaaatgtta gcttaatcaa
      601 ttaccttcct aaaaacaaaa tagaaactac tgatgttggt caaacattag gatataacat
      661 tggaggtaat ttccagtcag caccatctat aggtggcaat ggctcattta attattctaa
      721 aacaattagt tatacccaaa agagttatgt cagtgaagta gacaagcaaa actcaaaatc
      781 tgttaaatgg ggtgttaaag caaacgaatt tgttacgcct gatggaaaaa aatctgcgca
      841 tgatagatat ttattcgtac aaagtccaaa tggtccaaca ggttcagcaa gagaatattt
      901 tgctcctgat aatcaattgc cacctttagt tcaaagtggc tttaatccat cgtttatcac
      961 tacactatca catgaaaaag gttcaagtga tacgagtgaa tttgaaattt catatggtag
     1021 aaacttagat attacatatg cgactttatt ccctagaact ggtatttacg cagaaagaaa
     1081 gcataatgca tttgtaaata gaaactttgt agttagatat gaagttaatt ggaaaacaca
     1141 cgaaattaaa gtgaaaggac ataattaata tgaaaatgaa aaaattagtc aaatcatcag
     1201 ttgcttcatc aattgcactg cttttgctat cgaatacagt tgatgcagct caacatatca
     1261 cacctgtaag cgagaaaaaa gtagatgaca aaatcacttt atacaaaaca acagcaacat
     1321 ctgataatga taaattgaat atttctcaaa ttttaacgtt taatttcatt aaggataaaa
     1381 gttatgacaa agatacgtta gtacttaagg cagccggaaa cattaattca ggttataaaa
     1441 agcctaatcc aaaagattac aattactcac agttttattg gggcggtaag tataatgttt
     1501 cggttagttc agaatcaaat gatgctgtaa atgttgttga ctatgcacct aaaaatcaaa
     1561 atgaagaatt ccaagttcaa caaacattag gttattctta tggcggagat attaatatat
     1621 ctaatggctt atcaggtgga ttaaatggat caaaatcatt ttcagaaacg ataaattata
     1681 aacaagaaag ttacagaact acgattgata gaaaaacaaa tcataaatca attggctggg
     1741 gtgttgaggc gcacaaaatt atgaataatg gttggggacc atatggtaga gatagttatg
     1801 acccaacata tggtaatgaa ctgtttttag gcggtagaca aagtagttca aatgctggtc
     1861 aaaatttctt gccaacacat caaatgcctt tattggcgcg tggtaacttt aacccagaat
     1921 ttataagcgt actttctcat aaacaaaatg atacaaaaaa atctaaaatc aaagtaactt
     1981 accaaagaga aatggataga tatactaatc aatggaatcg actacactgg gttggtaata
     2041 actacaaaaa tcaaaataca gtaacgttta catctactta tgaagttgac tggcaaaacc
     2101 atactgttaa attaatcggt acggattcta aagaaactaa tcctggagta taactgttag
     2161 aaatagagga agttaaaccc gaaatttaag agttaaacaa aaaatattca caacatatta
     2221 aaaaaagaag ttacattatt tgtttaaact aacaatagta ccataaaatt aatgacgatt
     2281 tacaaattaa taacaaataa ttattattaa attcattgat ttattgtgtg gtaatgatta
     2341 atatgacaat tgaagtgggt cttaacttca acttttaaat agggggtttt atctttggaa
     2401 aaagttcttg atttagacgt gcaagttaaa ggaaataaca acactaatga ttcagcgggt
     2461 gacgaaagaa taactagcca tcttttttgt agctttggtt gtgaaaagac gggtagtttt
     2521 aacagcttct gttgttaagg atacagcaaa tagagcctga aagggctcta tttgtctttt
     2581 taaatatatt aagtgctaaa aaacggaaac agtttattaa tcgccaggta tttttataat
     2641 tagccttcta tattatcagt tgagtgtttc aataaattct gactttggtg atggaaagtt
     2701 atacttttag ttgtaatata gttttcatga cttcctgtta aataagttag gattatgtaa
     2761 ttagcctcta tctttacata taagtaatga caagctttgg ttattatatt gaccctctta
     2821 gaacatgtca tttaagaaaa taatcaagtc attgaaat
//
LOCUS       DALAGF010000169         2808 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.175, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000169 DALAGF010000000
VERSION     DALAGF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.175"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..378
                     /gene="lpl4"
                     /locus_tag="O2692_002666"
     CDS             <1..378
                     /gene="lpl4"
                     /locus_tag="O2692_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl4"
                     /protein_id="HCZ4300253.1"
                     /translation="ILLDKVEDKKLKQKIENFKFFSQYANLKELKNYSNGDVSINENV
                     PSYDVKYKMSNKDENVKQLRSRYNIPTDKSPVLKMHIDGNLKGSSVGDRKLEIDFSKR
                     ENSHLSVIDSLDYQPAKTNKDDE"
     gene            397..1167
                     /gene="lpl5"
                     /locus_tag="O2692_002667"
     CDS             397..1167
                     /gene="lpl5"
                     /locus_tag="O2692_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HCZ4300254.1"
                     /translation="MKSIKRIGLCISLLILSIFVTSCDGDNKITGDSKETQIKKSFSK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKGNKSDELDDEGMVLNLDR
                     NTRTAKGYYFVKKFYEKDKLPDRKNYKVEMKNNKIILLDKVEDPNLKKRIENFKFFGQ
                     YANFKDLENYNNGDVSINWNVPSYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHI
                     DGDLKGSSVGYKRLEIDFSKEGRDISVIDYLSYKPAKK"
     gene            1199..1990
                     /gene="lpl6"
                     /locus_tag="O2692_002668"
     CDS             1199..1990
                     /gene="lpl6"
                     /locus_tag="O2692_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl6"
                     /protein_id="HCZ4300255.1"
                     /translation="MEYLKRLALLISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLEDFYGKEGYRDGEFKKDDKGTWLIRSEIVKQPKGKVMKTRGMQL
                     YINRNTETAKGFFVLKEISENNNRVNKDKEEKYEVKMVGNKIIPTEQINDEKIKKEIE
                     NFKFFVQYGNFKNFEKYNNGEFSYNPEAPIYSAKYQLHNDDYNVRQLRKRYDISTKET
                     PKLLLKGGGDLKNSSVGQNDIEFTFVERKGENIYFNDSVEFIPSK"
     gene            2060..>2808
                     /gene="lpl7"
                     /locus_tag="O2692_002669"
     CDS             2060..>2808
                     /gene="lpl7"
                     /locus_tag="O2692_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl7"
                     /protein_id="HCZ4300256.1"
                     /translation="MKCFQKLYIFILILIVLMAGCESNKITGDSKETQIKKSFAKTLD
                     VYPTKNLEDFYDKEGYRDGEFKKGDKGKWVIRSEMTTELKNENMVSKGMVIRLNRNSR
                     TCTGEYFVRIVKEDSEGKVYSDERKYPVKMENNKIITLKPIDDEKVKKEIEEFKFFVQ
                     YGNFKELENYKDGEVTYNPEAPIYSAQYQLKNSDYNVEQLRKRYNITTKKAPKLLLKG
                     SGNLKGSSVGYKNIEFTFVENKEENIYFTDS"
BASE COUNT         1199 a          286 c          535 g          788 t
ORIGIN      
        1 attttattag ataaagtaga agataaaaag cttaaacaaa aaatagaaaa ttttaaattt
       61 ttcagtcaat atgcaaatct taaagaattg aaaaactaca gcaatggtga tgtctcaatt
      121 aatgagaatg ttccaagtta tgacgtgaaa tataaaatga gtaataaaga tgaaaatgtt
      181 aagcaattaa gaagtcgtta taatattcct actgataaat caccggtatt aaaaatgcat
      241 attgatggta atttgaaagg aagttccgtt ggagatagga agttagaaat tgatttttcg
      301 aaacgtgaaa atagccattt atcagtaata gattctttgg attaccagcc agcgaaaact
      361 aataaagatg atgaataatg aggatggtgt gtaacaatga agtctataaa aaggattgga
      421 ttgtgcataa gtctgttgat tttaagcatt tttgtaacat cttgcgatgg tgataataag
      481 atcactggag attcaaaaga aacacaaatc aaaaagagct tttcgaaaac gttagatatg
      541 tatccaatta agaatctcga ggacttatat gataaagaag gataccgtga tggcgaattt
      601 aaaaagggcg acaaaggaat gtggactata tatacagatt ttgctaaagg caataaatca
      661 gacgaattgg atgatgaagg tatggtttta aatctggata gaaatactcg aacggctaag
      721 ggatattatt ttgttaagaa attttatgaa aaggataaat tacctgatag aaaaaattat
      781 aaagttgaaa tgaaaaataa taaaattatc ttattagaca aggtagaaga tccaaatcta
      841 aaaaagagaa tagaaaactt taaatttttc ggacaatatg caaattttaa ggatttggaa
      901 aattacaaca atggcgacgt gtcaataaat tggaatgttc caagttatga cgtggaatat
      961 aaaatgagca ataaagatga aaatgttaaa caattaagaa gtcgttataa cattcctact
     1021 gataaagctc caatgttaaa aatgcatatt gacggggact taaaaggtag ttctgttgga
     1081 tataaaaggt tagaaataga tttttcaaaa gaaggtaggg atatttcagt cattgattat
     1141 ttaagttata agccagcgaa aaaatagtac tgataattaa atattagggt gtgaaggaat
     1201 ggaatatcta aaaaggcttg cattgttaat aagtgttatt attttgacca tttttataat
     1261 gggttgtgat agtcaaagcg atactgcaga aaatccaaaa gaaggttcaa aagaagcaca
     1321 aattaaaaag agtttttcga aaacgttaga tatgtatcca attaagaatc tcgaggattt
     1381 ttatggcaaa gaaggatatc gagatggcga atttaaaaaa gatgataaag gtacttggct
     1441 aattagatcc gaaatagtta aacagccaaa gggcaaagtg atgaaaacaa gaggtatgca
     1501 attatatatt aatagaaata ccgaaacagc caaaggtttc tttgttttga aagaaataag
     1561 tgaaaataat aatcgtgtaa ataaagataa ggaggaaaaa tacgaagtga aaatggtagg
     1621 aaataaaatt attcctactg aacaaattaa tgacgagaaa ataaaaaaag aaattgaaaa
     1681 cttcaagttt tttgtgcaat atggaaactt taaaaatttc gaaaaataca acaatggtga
     1741 gttttcatat aatcctgaag caccaattta ttcggctaaa tatcaattac acaacgatga
     1801 ttacaatgta aggcaactac gtaaaagata tgacatttca acaaaagaaa caccgaagtt
     1861 acttttgaaa ggtggaggag atttaaaaaa ttcctcagtt ggtcaaaacg atattgaatt
     1921 tacttttgtt gaaagaaaag gtgagaatat ttattttaac gatagtgttg aattcatacc
     1981 aagtaagtaa agttaaatta aaatgtgtgg ttagatgaaa tgatcaaata tcaaaaaatg
     2041 tgcaaagaga tgtgacaaga tgaagtgttt tcagaaatta tacatattta tattaatttt
     2101 aatcgtatta atggcaggat gcgaaagtaa taagatcact ggagattcga aagaaacaca
     2161 gatcaaaaag agctttgcga aaacattaga tgtataccct acgaaaaatc tagaagattt
     2221 ttatgacaaa gaaggatatc gagatggcga atttaaaaag ggtgacaaag ggaagtgggt
     2281 tattagatct gaaatgacaa cagaactgaa aaatgaaaat atggtatcta aaggtatggt
     2341 catacgttta aatagaaata gtagaacatg cactggtgaa tattttgtca ggatagttaa
     2401 agaagacagt gagggcaagg tatatagtga tgaacgaaaa tatccagtga aaatggaaaa
     2461 taataaaatc attacattaa aaccaatcga tgatgaaaaa gtaaaaaaag aaattgaaga
     2521 atttaaattc ttcgtacaat acgggaattt caaagaattg gaaaactata aagacggaga
     2581 agtgacatat aacccagaag caccaatata ctctgcacaa tatcaattga aaaatagtga
     2641 ttataatgta gaacaactac gtaagcgata taatataacg acgaaaaaag cgcctaaatt
     2701 attattgaag ggttcaggta atttaaaagg ctcatcagtt ggatataaaa atattgaatt
     2761 tacctttgtt gaaaataagg aagaaaatat ttacttcaca gatagtat
//
LOCUS       DALAGF010000170         2715 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.176, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000170 DALAGF010000000
VERSION     DALAGF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.176"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            58..657
                     /locus_tag="O2692_002670"
     CDS             58..657
                     /locus_tag="O2692_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCZ4300257.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            1025..1750
                     /gene="sasD"
                     /locus_tag="O2692_002671"
     CDS             1025..1750
                     /gene="sasD"
                     /locus_tag="O2692_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HCZ4300258.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(1942..2697)
                     /locus_tag="O2692_002672"
     CDS             complement(1942..2697)
                     /locus_tag="O2692_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCZ4300259.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
BASE COUNT          976 a          436 c          470 g          833 t
ORIGIN      
        1 aaacacaccc gaaattaatt attataaaag tatattcata aaaggaggaa tatacttatg
       61 gcatttaaat taccaaattt accatatgca tatgatgcat tggaaccata tatagatcaa
      121 agaacaatgg agtttcatca cgacaaacat cacaatacgt acgtgacgaa attaaacgca
      181 acagttgaag gaacagagtt agagcatcaa tcactagcgg atatgattgc taacttagac
      241 aaggtaccgg aagcgatgag gatgtcagtc cgtaataatg gcggtggtca ttttaaccat
      301 tcattattct gggaaatact atcacctaat tctgaagaaa aaggtggcgt aatagatgac
      361 atcaaagcgc agtggggcac tttagatgaa tttaaaaatg aatttgcaaa taaagcaaca
      421 acattatttg gatcaggttg gacttggtta gttgttaatg atggcaaatt agaaattgtg
      481 acaacgccaa accaagataa tccattaaca gaaggcaaaa caccaatctt actatttgat
      541 gtttgggagc atgcctacta tctgaaatat caaaataaac gtccagacta tatgactgca
      601 ttttggaaca ttgttaactg gaaaaaagtt gatgaattat accaagcagc aaaataatat
      661 aacttaatat aattgaggtg gagcatctac aaggtgttcc acttttttgt acttaatatc
      721 tttacttatt tgtttttgtg taatgggata gcacgtaaaa gtggaaaaat aataagataa
      781 atttcaactc gaaatacctt attattgttg actataacga tgacctaaat attatataca
      841 ttagacaaca ctattaatta atcaacatat tgacattaaa taaattgaca aaataagtaa
      901 ttattgtgaa tctaaagtga aatttttata aaaaaatgta atgattcaaa aattttgttg
      961 catttctttt gtaatcgtat gataatgtaa atgtaatcaa attgtaatat aaggggacaa
     1021 gacaatgaaa aaattagcaa cagtaggttc tttaattgta acaagcactt tagtattctc
     1081 aagtatgcct tttcaaaatg cgcatgccga cacaacttca atgaatgtgt cgaataaaca
     1141 aagccaaaat gtacaaaatc atcgtcctta tggcggagta gtaccacaag gaatgacgca
     1201 agcacaatat actgaattag agaaagcttt accccaatta agcgctggca gtaatatgca
     1261 agactataat atgaaattgt atgatgcgac gcaaaatatt gctgataaat acaatgtgat
     1321 aattacaact aatgtagggg tatttaaacc acatgctgtt agagatatga atggccatgc
     1381 gttaccttta acaaaagatg gcaattttta tcaaacgaat gtagatgcaa atggtgttaa
     1441 tcatggtggt agtgaaatgg tgcaaaataa aacaggtcat atgagtcaac aaggccatat
     1501 gaatcagaac acacacatga accaacagcc acacatgcaa caaggtcata tgcaatcatc
     1561 aaaccatcaa atgatgagtc caaaagcaaa tatgcattca tcaaatcatc aaatgaacca
     1621 aagtaacaaa aaagttttac cagctgctgg tgaaagtatg acatcaagta ttcttactgc
     1681 aagtattgcc gcactactat tagtatctgg gttattctta gcatttagac gacgttcaac
     1741 aaataaataa acataatacg attaataata gaaaaatcgt gtgattatct gagcgagcct
     1801 aggacataaa tcaatgtcct aggctcgcta atgttatatt ggcagtagtt gactgaatga
     1861 aattgcgctt gtaacaagct tttccatttc ttaccaactc cctaaatagt catcaaaaaa
     1921 tttcttatat tttaataatt tttaataatc cgattgtctt atacgtgtca gtgttaattc
     1981 agatatttcc tgtggaatat accacttatt aatcataatt ggataaggtg tttgtgcgta
     2041 cagtgtttca ataatcagcc aacaatgtgt atcaccatca aacacgtgac tatgattttt
     2101 gaagtggggc gctttggtaa tagacatttt taaatctgat tgatatgcat tgctataaat
     2161 cgtttgctca acgaatgtct tcatgtcgtc ttcgttttgt gtattcactt taaatgtgtc
     2221 aatgacattt aacggtataa aggtaaagca aaatgcatca gcttgcttag aatgattgtc
     2281 ctttttttga taatagcgtt ccattgcaat gacggcagaa ggatggtttg caaacaaatg
     2341 atttgtatat tcactttcta aatcaacacg ataattaatt gatgacatag atacgcgagc
     2401 tagcaatatt tgatcaagtg gatgcttaaa ttgatccata cttgaagcgt gttgggcatt
     2461 tgtttgtgga ataacaaagt gtcccttccc tcttgtactc tctacgatgc catcttcggc
     2521 taacaatttt atagcttggc gcaaagtcat acgactgaca tcaaagcgcg cacaaagttc
     2581 cttttcagta ggtaatgcat ggccactcgg atattttcct atttgaattt ctttatataa
     2641 cgtattataa atcgttaaaa attttggttg tgtttgcgtc acgtagacaa cctccataaa
     2701 gttacttaat cactc
//
LOCUS       DALAGF010000171         2713 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.177, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000171 DALAGF010000000
VERSION     DALAGF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.177"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..513
                     /locus_tag="O2692_002673"
     CDS             <1..513
                     /locus_tag="O2692_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCZ4300260.1"
                     /translation="KKYIIPWALLGFALVMIYQMVVSIVLTQIYGGQQVSPNTEKLII
                     IARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGNRIVAFIIATTVSSLIFALA
                     HNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQNGFVVIFQLLNPEALKKATEQ
                     ANFIYHIFIP"
     gene            complement(538..1791)
                     /locus_tag="O2692_002674"
     CDS             complement(538..1791)
                     /locus_tag="O2692_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300261.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPKPDPDKPKPNPDPKPDPDNPKPNPDPKPDP
                     NKPNPDPKPDPDKPKPNPNPKPDPNKPNPNPSPDPDQPGDSNHSGGSKNGGTWNPNAS
                     DGSNQGQWQPNGNQGNSQNPTGNDFVSQRFLALANGAYKYNPYILNQINKLGKDYGEV
                     TDEDIYNIIRKQNFSGNAYLNGLQQQSNYFRFQYFNPLKSERYYRNLDEQVLALITGE
                     IGSMPDLKKPEDKPDSKQRSFEPHEKDDFTVVKKQEDNKKSASTAYSKSWLAIVCSMM
                     VVFSIMLFLFVKRNKKKNKNESQRR"
     gene            1988..2614
                     /locus_tag="O2692_002675"
     CDS             1988..2614
                     /locus_tag="O2692_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCZ4300262.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIAASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
BASE COUNT          821 a          413 c          476 g         1003 t
ORIGIN      
        1 aaaaaatata tcattccatg ggcattgctt ggatttgcat tggtaatgat ttatcaaatg
       61 gtagtgagca ttgtattaac gcaaatttat ggtggacaac aagtaagtcc taatacagaa
      121 aagctaatta ttattgctcg aaaaatacct atatttatct tctttgtatc tattattggt
      181 cctttattag aagaatatgt attcagaaaa gtaatctttg gagaattatt taatgcgatt
      241 aaaggtaatc gtatcgtggc atttattatt gctacaacag taagttcatt aatatttgca
      301 ttagcacata atgatttcaa atttattcca gtttattttg gtatgggtgt cattttttca
      361 ttagcatatg tttggacaaa acggcttgct gttccaatta ttatccatat gttacaaaac
      421 ggatttgtcg ttatattcca attacttaat cctgaggctt tgaaaaaagc cacggaacaa
      481 gcgaatttta tatatcacat ttttattcca taataaaaaa aacgaatcac acacggatta
      541 tcgtcgctgt gattcgtttt tatttttctt tttatttcgc tttacaaata agaatagcat
      601 gattgaaaat accaccatca tagaacatac aattgctagc caacttttac tatatgcagt
      661 tgacgcactt ttcttattat cttcttgttt tttaactact gtaaaatcgt ctttttcatg
      721 cggttcaaat gagcgttgtt ttgaatccgg cttatcttcg ggctttttca aatctggcat
      781 tgatccaatt tcaccagtaa ttaatgcgag tacttgttca tctaaattac gatagtacct
      841 ttctgatttc aatggattga aatattggaa tctaaagtaa ttcgattgct gttgtaatcc
      901 atttaaatat gcatttccgc tgaaattttg ttttcgaata atattataaa tgtcttcatc
      961 agtaacttct ccataatctt tgcccaactt attaatttga tttaaaatat acggattata
     1021 cttgtaagcc ccatttgcca aggctaaaaa tcgttgggat acaaaatcat taccagtagg
     1081 attttgtgag tttccttgat tcccatttgg ttgccattga ccttgattag atccatctga
     1141 agcatttggg ttccatgtcc ccccattttt cgagccacca gaatgattgg aatccccagg
     1201 ttgatcggga tctggtgacg ggttaggatt tggcttatta gggtctggtt ttggattcgg
     1261 atttggcttt ggtttatctg gatctggttt tggatccgga tttggcttat tagggtctgg
     1321 ttttggatct ggatttggtt tcgggttatc tggatctggt tttggatccg gatttggctt
     1381 tggtttatct gggtctggtt ttggatccgg ttttggtttc gggttatctg gatctggttt
     1441 aggattcgga cttgggtttt ggttatttgg gtctggtttt gaatctgtac ttgggttttg
     1501 gttgtttgag tctggcttca cattattcga atcaggatta gcatcacgac tattcgaacc
     1561 tgttccttca ttacccgttt gatttgattc gtgaggtgtc ccagtctcat tattattcat
     1621 ttcactattt actgatggtg aagttatatt atgattcaaa ttagtagttg gtttttctcc
     1681 atttaagtta tcctttgctt caactgtgtg cgttgcaagc cctaaactac tactacttaa
     1741 aattaaaata cttgttgaaa gtaacatttt cttaaaccaa tgatatgaca tttcacgaga
     1801 cacctccctt tttcatgtaa gtattaatct aagtataata aatatgcgtt gaaaatgcat
     1861 ttatatttgg aatttgaaaa tgaatattaa tattcttgaa attttcgtaa atattgcttt
     1921 acaagtacga gtataaattt tatacttagt ctattaagta ttctttacta aagaaggagc
     1981 aataattatg ggcttattta aaaaagataa aaaagcaatg acttttgaca atgcaatgga
     2041 agaaagacgt tcaatatata atttgaaaga ttcgatatca attagcgatg acgaattaga
     2101 atcaatgatt gcacatgcag ttaaacatgt tccttcatca tttaattctc aatcaactag
     2161 aattgttctc ttgttaaatg acaacaataa taaattttgg gataacacaa aagcaatttt
     2221 aaaagaagtc atgggagaga atcgtgattt cgagccaact gaacaaaaaa ttgataactt
     2281 caaacattca tacggtacaa ttttgtttta tgaagatcaa gatgttgtaa gtggtttaca
     2341 agaacaaatg ccaaactact atgataattt tgcaatatgg tcaactcaaa caaatgccat
     2401 gcatcaattt gcaatttgga ctgcattagc tactaaaggc atagctgctt cattacaaca
     2461 ctataatcca cttgttgatg agatgacttc aaatgaattt aatattccta aaagttggaa
     2521 acttattgct caaatgccat ttggtgatat tcgtgaagct gctggcgaaa aaacatttaa
     2581 tccagtggaa gatcgtttcg taataaaaaa ataatcaata tattgttagg aatgggacag
     2641 aaatgataaa gaatcattag ttattcatta tgtagtagtt cttacacatt agccgctgct
     2701 aatgtgcact taa
//
LOCUS       DALAGF010000172         2667 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.178, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000172 DALAGF010000000
VERSION     DALAGF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.178"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            183..890
                     /gene="deoD"
                     /locus_tag="O2692_002676"
     CDS             183..890
                     /gene="deoD"
                     /locus_tag="O2692_002676"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCZ4300263.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            897..2249
                     /gene="tet(38)"
                     /locus_tag="O2692_002677"
     CDS             897..2249
                     /gene="tet(38)"
                     /locus_tag="O2692_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCZ4300264.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVRLVLFVSLNRWTQSEK
                     "
     gene            2330..>2667
                     /gene="deoC"
                     /locus_tag="O2692_002678"
     CDS             2330..>2667
                     /gene="deoC"
                     /locus_tag="O2692_002678"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCZ4300265.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVV"
BASE COUNT          866 a          394 c          497 g          910 t
ORIGIN      
        1 ttataaaaat taaatataga taggctttga aaattagttt tatacaaggt tagtagctgt
       61 aactgtaaaa tgttcttaat attgtcaaaa tgtaatgctt gaaagcgctt ttaaaaaata
      121 ttattatata catggttaga caaatagaca aatcactata caaatattgg gaggaatatt
      181 ttatgaaatc aacaccacac attaaaccaa tgaatgacgt cgaaattgca gaaacggttc
      241 tattgccagg agatccgtta agagctaagt tcattgcaga aacttatttg gatgatgtgg
      301 aacagttcaa tacagtgcga aacatgtttg gttttaccgg aacatataaa ggtaaaaaag
      361 tttctgtcat gggttcaggt atgggtatgc catctattgg catttactct tatgaattaa
      421 ttcatacatt tggttgtaaa aaattaattc gcgttggctc ttgtggcgcg atgcaagaaa
      481 acattgattt atatgatgtg attattgcac aaggtgcctc tactgattca aattacgttc
      541 aacaatatca attaccaggt cattttgcgc caattgcttc ttatcaatta ttagaaaaag
      601 cagttgaaac agcacgtgac aaaggtgtac gtcatcatgt aggtaatgtg ttatcaagtg
      661 atattttcta taacgcggat acaacagcga gtgaacgttg gatgcgtatg ggtattttag
      721 gtgtagaaat ggaatcagct gcattataca tgaatgcaat ttacgctggt gtcgaagcat
      781 taggtgtgtt cacagtgagc gatcatttaa ttcatgaaac gtcaacaaca cctgaggaaa
      841 gggaacgtgc atttacagat atgattgaaa ttgcactgtc attggtgtag atgattatga
      901 atgttgaata ttctaaaata aagaaagcag tacctatttt attattctta tttgtattca
      961 gtttggttat agacaactca tttaaattga tttctgtagc cattgctgat gacttaaaca
     1021 tatctgtaac gacagtaagt tggcaagcga cattagccgg tttagtaatt ggtattggcg
     1081 ctgtagtata cgcttcatta tctgatgcca ttagtatacg cacactattt atttatggcg
     1141 tgatattaat cattatcgga tcaattattg gttacatttt ccaacatcaa ttcccattac
     1201 ttttagttgg acgtattatt caaactgccg gtttagctgc tgcagagaca ttatatgtga
     1261 tatatgttgc aaagtatctt tctaaagagg accagaagac ttaccttggc ttaagtacga
     1321 gcagttattc cttgtcatta gttatcggta cattatcagg tggatttatt tctacgtatt
     1381 tacactggac aaatatgttt ttaattgcat taatcgtagt atttacgttg ccattcctat
     1441 ttaaattatt accaaaagaa aataatacga ataaagctca tttagatttt gttggcttaa
     1501 ttctagtggc aactattgct acaacagtca tgctgtttat tacgaacttt aattggttat
     1561 atatgattgg tgccttaatt gcgattatcg tttttgcgct atatattaaa aatgcgcaac
     1621 gtccattagt aaataaatca tttttccaaa ataaacgtta tgcttcattt ttatttatag
     1681 tatttgtaat gtatgctatc caattgggtt atatttttac gttcccattc ataatggagc
     1741 aaatttatca tctgcaacta gacacaacat cactgttatt agtaccgggt tatatagtag
     1801 cagtcattgt tggtgcacta agtggtaaaa tcggcgaata tctgaattca aaacaagcga
     1861 ttatcacagc aattatttta atagcactga gcttgatttt acctgcattt gcagtaggta
     1921 atcacatttc aatcttcgtc atttctatga tattctttgc aggtagcttt gctttaatgt
     1981 atgcaccttt acttaacgaa gccattaaaa caatagatct taatatgaca ggtgtggcta
     2041 ttggttttta taatttaatt attaatgtgg cggtatctgt aggtattgcg attgctgcgg
     2101 ctctaatcga ttttaaagca ttaaatttcc caggcaatga tgcattaagt tcacatttcg
     2161 gtattatttt aattatttta ggtttaatga gtattgtcag attagtttta ttcgtcagct
     2221 taaatcgttg gacacaatct gaaaaataaa tagatataaa ttcgcgagat atattcgtat
     2281 ttatagtaaa attaaataaa gagattatat aacacgagga gtagtaagta tgaaatttga
     2341 gaaatatata gatcacactt tattgaagcc tgagtcaaca cgtacgcaaa tcgatcaaat
     2401 catcgatgaa gcgaaagcat acaattttaa atctgtatgt gtgaatccaa cacatgttaa
     2461 atatgcagca gagcgactag ctgattcaga ggtgctcgtt tgtacggtaa taggattccc
     2521 attaggtgcg tcgacaactg caacgaaagc atttgaaaca gaagatgcaa ttcaaaatgg
     2581 tgcagatgaa attgacatgg tcatcaacat cggcgcatta aaagatggac gttttgatga
     2641 tgtacaacaa gacattgaag cagtggt
//
LOCUS       DALAGF010000173         2659 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.179, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000173 DALAGF010000000
VERSION     DALAGF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.179"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(78..269)
                     /locus_tag="O2692_002679"
     CDS             complement(78..269)
                     /locus_tag="O2692_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4300266.1"
                     /translation="MPEQLSVRKWRLVRDLKQQEVADILGVNAKTVGHWEKDDTNLSN
                     VTVYALAKLYDIEVDQIKV"
     gene            430..759
                     /locus_tag="O2692_002680"
     CDS             430..759
                     /locus_tag="O2692_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4300267.1"
                     /translation="MENNKVRKILSENLQELMNDKNIDQRELAEAIGVSQPTVSNWIQ
                     QTKYPRIKRIQQLADYFNVPKSRITESKKDIHQETIAAHFDKEGLTEEEIEEVNRFIE
                     WVRNRDK"
     gene            772..1233
                     /locus_tag="O2692_002681"
     CDS             772..1233
                     /locus_tag="O2692_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCZ4300268.1"
                     /translation="MGLYEELCINNEKIKIEETDQLPNFQPGCYMNGKIYIRRNLSEV
                     RKAEVLYEQLAHHKLTYGNILDQTKWINRKFENYARRHGFTSAVPLHEIVEAHNYGVR
                     NLYELSEYLQLSESYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            1251..1685
                     /locus_tag="O2692_002682"
     CDS             1251..1685
                     /locus_tag="O2692_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300269.1"
                     /translation="MKKVIGLLLVSTLALTACGEKEKPKKEENKKSQTQNHKDSKPKT
                     QQEKMKKVEDKNPPNNSIQNNSNNQNQSQNNQLNNNSDPSNNTPANINENDSQNTNLN
                     DEYVVSPGWTKDEQAKAFEEYKKGKEEEARAGASAVPGANIN"
     gene            1714..2109
                     /locus_tag="O2692_002683"
     CDS             1714..2109
                     /locus_tag="O2692_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300270.1"
                     /translation="METNKTIDLMNYVEFPKRYTEAKGKLVAQPITTINSARRVENED
                     MTVCYILDQDDDVMDFIFDRDIITVYCPENGTATDEYFCEIIFNSDDTFTLKRLSNYV
                     TIKDRSYPMSKINDVNITGKVVRLFRDFK"
     gene            2199..2345
                     /locus_tag="O2692_002684"
     CDS             2199..2345
                     /locus_tag="O2692_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300271.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(2342..>2659)
                     /locus_tag="O2692_002685"
     CDS             complement(2342..>2659)
                     /locus_tag="O2692_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCZ4300272.1"
                     /translation="IIWLINITRKFMKLDCISLLDKRDKLFNNNGKPVFIVIKDFENR
                     IIEEGELKTYNSAGSDFDLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQIKLDLYLM
                     NEY"
BASE COUNT         1065 a          403 c          421 g          770 t
ORIGIN      
        1 ttcctccttt taatttgttt atgtccattt gttacattac ctaaaagtta taaaaagtta
       61 tacgaaggtc aaaaaattta gacctttatc tggtctacct caatatcata taactttgct
      121 aaagcgtaaa ctgtaacatt acttaaatta gtgtcatcct tttcccaatg accaactgtc
      181 tttgcattga cgcccaatat atctgctact tcttgctgtt tcaagtccct tacaagtctc
      241 cattttctta cggataattg ttccggcatg tcgtccacct ccctttcaca cgtataactt
      301 tatattactt tgtattactt gtcaacagat aaagttatat ttcttcataa aaagttataa
      361 aaagtattgt aaagtaatgt cggttatggt aagttaatat tacattaagt aatattaaag
      421 gagacaacta tggagaataa taaagtcaga aaaattttat ctgaaaacct tcaagaactt
      481 atgaatgata aaaatattga tcagagagaa cttgctgaag ctattggagt ttctcaacct
      541 acagtctcca attggattca acaaactaaa tatccacgaa ttaaaagaat tcaacaactt
      601 gcagattact tcaatgtacc gaaatcaaga attactgaat caaaaaaaga tatacatcaa
      661 gaaacaattg ctgctcattt tgataaggag ggattaactg aagaagagat tgaagaagta
      721 aatagattca ttgaatgggt tagaaataga gacaaataaa gggtgtttat attgggatta
      781 tacgaagaac tttgcataaa taacgaaaaa ataaagatag aagaaactga ccagcttcca
      841 aatttccaac ctggatgcta tatgaacgga aaaatttata taaggcgtaa tttatcagaa
      901 gtacgtaaag cagaagtgtt atatgagcaa cttgcccacc acaagttgac gtatggcaac
      961 attttagacc aaacaaaatg gattaataga aaatttgaaa attatgcacg tagacatggt
     1021 tttacttcag ctgtaccgct acatgaaatt gtagaagctc acaactacgg tgttcgtaat
     1081 ttgtacgagt tgtcagagta tctgcagttg agtgaatcat acatactaga agctatagaa
     1141 caatataaaa agatatatgg tattggaact cactatggcg agtattctat tacgtttgag
     1201 ccgttgagag tttttaaata taaggaaata taaacaaagg agagatacat atgaaaaaag
     1261 taatcggact gctactagta agtacattag ctttaacagc ttgtggtgaa aaagaaaaac
     1321 caaaaaaaga agaaaataaa aagtcacaaa cacaaaatca caaagatagc aaaccaaaaa
     1381 cgcaacaaga aaaaatgaaa aaagttgaag ataaaaatcc acctaataat agcatacaaa
     1441 ataattcaaa caatcaaaac caatcacaaa acaatcaact taataataat tcagatccat
     1501 ctaataatac tcctgcaaat ataaatgaaa acgattcaca aaatactaat ttaaatgatg
     1561 agtatgtcgt ttcgcctggc tggactaaag atgaacaggc taaagctttt gaagagtaca
     1621 aaaaaggaaa agaagaggaa gcaagagctg gtgctagcgc agtaccagga gccaatatta
     1681 actaataaaa caatataaga aagaagagct aatatggaaa caaataaaac aatcgattta
     1741 atgaattatg tggaatttcc aaaaagatac acagaggcaa aaggcaaatt agttgcacaa
     1801 ccaataacta ctataaatag cgcaagaaga gttgaaaatg aagatatgac tgtttgctac
     1861 attttagatc aggatgatga tgtaatggac tttatctttg acagagatat aattactgtt
     1921 tactgtcctg aaaacggaac tgcgactgat gaatattttt gtgaaattat atttaactca
     1981 gatgacacat ttaccctaaa gcgattatct aattacgtta ccattaaaga tagaagctac
     2041 ccaatgtcaa aaataaatga cgtaaacatt acgggcaaag tcgtcagatt atttagagat
     2101 tttaaataaa cttggcttta attacgatta aaagtaccta tatagcgtga cgagaaaaag
     2161 gattaaaaaa aatcaaaaaa cgcctactag tgtagacgtt gaatggtggt gagaatttta
     2221 tggtagataa aaacaaaaaa caagaaacta ctcgtagtaa cccattaaac aaaagttttg
     2281 aaaagtcagg cgccagcgaa aaattaaaaa gcactttatc agaaaaagct aagaaaaaag
     2341 attagtattc attcattaaa tataaatcca atttaatttg ttgtttaagg tctacaagcg
     2401 tatgtttaat atacaattca tcgtttgacg gtaaatcaga tactttgaaa tcttgtcgct
     2461 caacttctag taaatcgaaa tcgctaccag ctgaattata ggttttaagt tcaccctctt
     2521 caatgattct gttttcaaag tcttttataa ctataaatac tggtttaccg ttgttattaa
     2581 acaacttgtc tcttttgtct aataagctta tacaatccaa tttcataaac tttctggtta
     2641 tattaattaa ccagataat
//
LOCUS       DALAGF010000174         2591 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.180, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000174 DALAGF010000000
VERSION     DALAGF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.180"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(87..1223)
                     /locus_tag="O2692_002686"
     CDS             complement(87..1223)
                     /locus_tag="O2692_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCZ4300273.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(1255..1884)
                     /locus_tag="O2692_002687"
     CDS             complement(1255..1884)
                     /locus_tag="O2692_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCZ4300274.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(1903..2172)
                     /locus_tag="O2692_002688"
     CDS             complement(1903..2172)
                     /locus_tag="O2692_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCZ4300275.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            2335..>2591
                     /locus_tag="O2692_002689"
     CDS             2335..>2591
                     /locus_tag="O2692_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCZ4300276.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYS"
BASE COUNT          771 a          409 c          405 g         1006 t
ORIGIN      
        1 taatagaaat cagctttttt acattgccta agaactttaa ggaacttaat gtcccaagct
       61 cttttttgtt atatctaatt cgtaatttat gattgtttat tcggtccttt gatgttcact
      121 aaatgtgact ttaaatcttg ttctaattgt tgtaattctt tttcagctaa ttgtcgttct
      181 tcgcgaccgt gttgttgaat aattaatgtt tcttcaattg tctcaataat gttacgctgt
      241 gtacgtttca atgtatcaag atcaacaatg ccacgctcat tttctgttgc agtttcaatc
      301 gcattttgtt tcaacatttc agcatttgct gttaataaat cattagttgt atcagtgaca
      361 gctcgttgtg cagcaactgc attacgctgt ctcattaatg taagcgcaat ggccatttga
      421 tttttccata gtggaatact tgtcaaaatt gaactttgta tcttctcggc aagtgcttga
      481 ttaacatttt gaatcatacg aatttgtggc gcagtttgca aagctatttg tcttgaaagc
      541 tgtaagtcat atatgcgttt atctagtcta tctataaatt gctgcatatc tgcaacttgt
      601 tgaatatcca tttgattagt ggattgctgc gcttgctgtt gcaattgtgg tagcttttca
      661 ttttctaatt gcaacttttt ttgctgtgca gcaatgatat gcaatgataa gtcatcaaag
      721 tattgtttgt ttttatcata tagcgtatct aataattcaa tatctcttgt taaatgtgtt
      781 tgatgtttct gcagttgaat cgttatgcga tcgacttgag cactaactga ttgcattctt
      841 gaaaagattt cattgataga cgactttgct ctgctaaaaa ttctttttaa catagatggt
      901 ttatcagtat ttaactcatt tggattaact gactttagtt ttgacattag atctgacaaa
      961 gtatctccaa taggaccaac atctttactt tgtacttcat ccaacatttg atgtgaaaat
     1021 tgagacattt gtttctgtaa atcagaacca aacgctaata aaccttcatt gtctaaaggg
     1081 ttaatttgtt tactgattgt gtctacctgt ttttgttgtt caattgttaa cttttctttt
     1141 tgaatagtag tattcgataa ttctttatcg cttataaaat catctagtgg atggctttct
     1201 ttgaaacttt tattttcagt catcaatcat ctctccaata gttgctattt tacatcaatc
     1261 ttgatgatgt ttttgatgta acttatttaa ttccatttca atatctaaac gttcataatc
     1321 atcttcgttg agacgcttta aatcagcgat taatgttcgt ttgacctcat ccaaagtaat
     1381 tcgtgtttgt tctaactttt gctgttcatt aattgatttt ttgggcattt ttgctagacg
     1441 tgtatatgca tcaaccaaat ttaaagcatt atcaatatga gaataaaaaa agccttcaac
     1501 tttataaaat gatgcaggtc tctgtctaac tgtcgtataa atagaacgtg aaatttggta
     1561 tatatcatta atctgcctaa aatctttaat tgatcttata ttgacatacg tttttaaaat
     1621 acctctaagt ttttggtgtg tatgatttaa ctgatttcga atatagcgat agtcttttct
     1681 agtcaaacca atttcgttta aatatttgcg tgaagtgagt ttttgtatcg gtaggtatgt
     1741 cattaaaaag ccaacaatac caatagacat atcaattaaa aaagatacat caagtgcaat
     1801 catcccaaat atgcttgtta aaaacgctac aggaattccc actaacaccc caaatatatg
     1861 agaaatatta tatctcactg tcatcttcct ttatttagca ttttatattg atcgaaaatc
     1921 cgataatttt tgatttagtt ctaactcttc aagttgatgg cttgttacat ttgatgctgg
     1981 tgaggcacct tcaattacac cttgaataaa tctctctata tctgcgtcat ccccttgtgc
     2041 atatatctct acatagtcat ctacattttg aacagtaccg acaatgttat agttcattgc
     2101 aatgcgttgt gtaaaatatc taaatccgac gccttgaacg cgtccgaata cttgtaaatg
     2161 tatatgtctc atttttacca cctcataatg ttattatacg tagttttact taaaaaaact
     2221 aataattact atagttacta ctttgtttgt ttcaagtcgt caaacttgat tttcagagga
     2281 taaaggtata aaaataagta tagagttttt gaagtatgga aggggtcttt aataatgtgg
     2341 acagttacca aaattagagc cgattatgag ggatggtggt tattcagtga ctggccagaa
     2401 aacattgttg aaaaatatca atatcaagat tttgatgaca tgtttaagca ctatcaacaa
     2461 ttgattaatc aatgtaaagt tcagttcgat aactatgtca caggcaaata taatatttat
     2521 gcattttata ataattgtga catgaactat tgcgaagatt gtgaagagga tttacaaata
     2581 ttttatagtt t
//
LOCUS       DALAGF010000175         2461 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.181, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000175 DALAGF010000000
VERSION     DALAGF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.181"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..809
                     /locus_tag="O2692_002690"
     CDS             <1..809
                     /locus_tag="O2692_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCZ4300277.1"
                     /translation="GEEILKKTEYLTSFIQSYGEISLERAYYLVENYIDENCDDKVAE
                     IYKFEFQRKYDNGEFDFNHGYNFELNMLIDHYKRDVKDYDNARKYSNIYLYFGLRDFL
                     KKLMSNYSYYDSKGNIDLNEIQNDLNRFINSSASGMYERLIYNENLSNNIMFELFKKD
                     TQDYSDLEEQLIEKFINYVVSNVKKESRSNTLIELSKILENGYTIDKEEFKKEDDYLS
                     KYIFTDIDYLKKLESKINVAIDIRSGEIHLVLDDDSLDILIQNQKYGNEF"
     gene            852..1335
                     /locus_tag="O2692_002691"
                     /pseudo
     CDS             852..1335
                     /locus_tag="O2692_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837446.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1496..1600
                     /locus_tag="O2692_002692"
     CDS             1496..1600
                     /locus_tag="O2692_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300278.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            complement(2100..>2461)
                     /locus_tag="O2692_002693"
     CDS             complement(2100..>2461)
                     /locus_tag="O2692_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCZ4300279.1"
                     /translation="KTPVGYLISFNTQSLSQSVAENIIRVYTVMTIILFVVIHAIILM
                     GIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVETKTEKAQDSLND
                     NTTKTMTSDKPEDNQPK"
BASE COUNT          895 a          297 c          435 g          834 t
ORIGIN      
        1 aaggagaaga aattttaaag aaaacagagt acttaacctc ttttatacaa agttatggtg
       61 aaatttctct tgagcgtgct tattatttgg ttgaaaacta tatagatgaa aattgcgatg
      121 ataaagttgc agaaatatac aagtttgaat ttcaaagaaa atatgacaat ggcgagtttg
      181 attttaatca tggatataat ttcgaattga atatgttgat agatcactat aaaagagatg
      241 taaaagacta cgataatgcc agaaagtatt caaatattta tctttacttt ggtttgagag
      301 atttcttaaa aaaattaatg agtaattatt catattacga tagtaaaggg aatatagatt
      361 tgaacgaaat acaaaacgat ctgaatagat ttatcaactc tagcgcttct ggtatgtacg
      421 agcgattaat atataatgaa aatttatcca ataatattat gtttgaatta tttaaaaagg
      481 acacacaaga ttatagtgat ttggaagaac aattgattga aaagttcata aactatgtag
      541 tgtctaatgt aaaaaaagaa agtaggagta atactcttat agagttatca aaaattttag
      601 agaacggata tacaattgat aaagaagaat ttaaaaaaga agatgattat ctttctaagt
      661 acatatttac tgacatagat tatttgaaaa agttagaatc aaaaataaac gttgctattg
      721 atattcgaag tggagaaatt catttggtat tagatgatga tagccttgat atattaatac
      781 aaaatcaaaa atacggcaat gagttttgag tcataactaa atataatatg ttgaagagag
      841 gttttattta aatggctaaa attggttatg atcgtgtatc aacgaaagat taatatcgat
      901 acacttaaag aatttgggtg tgggtgtgta tatagtgaaa gaataagtgg tgtcaaacgt
      961 acagagcttg ttaagtgttt agattattta cgagagagcg acacattagt tgtctatcaa
     1021 cttgatcggt taggtagaac gacaaaacta ttaattgaat tatcacaatg attcgatgat
     1081 aacggaattg acttacaaat tagtaacatg aacatttcaa cgaaagacac aatgggcaaa
     1141 atgtttttta cgatgatgag tgcatttttc aggattagaa gttaatttac tatgtgagtg
     1201 tataaaatag acttagcagc aacaagagcg agaggccgaa aaagcgggcg cccctcttta
     1261 ccagagaata aaaaatgaga aattaaattt ttatatgatg aacaaacgat aacaggggaa
     1321 gaaatagcta gttagacaag ggtatgtcac tcaactgttt atcgagttga ttaagaaatg
     1381 aaaaaactta tactatgaat tactgtttaa aagtgtgcat gttataatat ttattgagca
     1441 agttggatag atggtggcta atctcttaat aaaggggtga tgcctatggt tatagttgtt
     1501 actcctagaa aggactagca tgtctgattt tgaaatgctt atggttgtat taacaatcat
     1561 tggtttagta ttgattagta ctcaagacca taaaaaataa ccttctattc gctttgaccg
     1621 gcatttttga aggctatttt taaataaaat ataaggtcac cgtcttttta acgggctcat
     1681 tagggtaaca tgtttccgag tgttgccctt tttgtgtttc aagagttaat gattttcatc
     1741 ttttgcttct acttactaca aatatatttt aacacatttt tcttatgaat tgtagttctg
     1801 aacataatca gaattaataa aaccaacttt ccatacagca gaaaatacaa ttaaaagtat
     1861 agaatgtaat cacgaattat ataaaaagta ttggagacct cttcatatat agataattca
     1921 cttagttatt ttagaaagaa gcccctaaca actaaagttg aaaaatagag gaacacagtt
     1981 ggatgctacg catcaactgc ataagagccc ctaacaacta aagtttaaaa gtaggagcgc
     2041 ctgcgctaag cgcatgcata agagccccta acaactaaag ttgtaagggg ctctaaaatt
     2101 tattttggtt gattgtcttc tggtttatct gaagtcattg tttttgttgt attatcattt
     2161 aatgaatctt gtgctttttc tgtttttgtt tcaacagatg ttgtcacttt atcattttct
     2221 acttttgtat tttttggttc agtgacaact tttttattct tgtcagctgt ttttacttta
     2281 tctttagtaa ttttttgacc acgttttaag taatattgaa cgatacccat taaaatgatt
     2341 gcatgaataa caacgaataa aataattgtc attactgtat aaactcttat gatattttct
     2401 gcaacacttt gagagagtga ttgcgtatta aatgaaatca agtaaccaac aggtgtttta
     2461 a
//
LOCUS       DALAGF010000176         2408 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.183, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000176 DALAGF010000000
VERSION     DALAGF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.183"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..328
                     /gene="lpl1"
                     /locus_tag="O2692_002694"
     CDS             <1..328
                     /gene="lpl1"
                     /locus_tag="O2692_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl1"
                     /protein_id="HCZ4300280.1"
                     /translation="QKIENFKFFGQYANLKELRKYNNGDVSINENVPSYDVEYKMSNK
                     DEIVKELRSRYNISTEKSPILKMHIDGDLKGSSVGYRKLEIDFSKRENSKLSVIEFLS
                     YKPAKK"
     gene            359..1159
                     /gene="lpl2"
                     /locus_tag="O2692_002695"
     CDS             359..1159
                     /gene="lpl2"
                     /locus_tag="O2692_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl2"
                     /protein_id="HCZ4300281.1"
                     /translation="MRYLKKLAWFISVIILGIFIIGCDSSSDTGEKAKEDSKEEQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWMIYTDFAKSNKPGVLDNEGMIL
                     NLDRNTRTAKGYYFVDTIYDNHENSYSKNYRVEMKNNKIILLDKVEDQKLKERIENFK
                     FFGQYADFKSLKSYNNGDVSINSNVPSYDAKFKMSNKDENVKQLRSRYNIPTEKAPIL
                     KMHIDGDLKGSSVGYKKLEIDFSKEENSELSVVDSLNFQPAKKNKDDE"
     gene            1178..1985
                     /locus_tag="O2692_002696"
                     /pseudo
     CDS             1178..1985
                     /locus_tag="O2692_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            2034..>2408
                     /gene="lpl4"
                     /locus_tag="O2692_002697"
     CDS             2034..>2408
                     /gene="lpl4"
                     /locus_tag="O2692_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl4"
                     /protein_id="HCZ4300282.1"
                     /translation="MGYLKRLVLYIVIMVMSVFIIGCDKSSDTAEKSKEDSKETQIKK
                     SFAKTLDMYSIKNLEELYDKEGYRDGEFEKGDKGMWTIYTDFAKSNKPGELSNEGMVL
                     YLDRNTRTAKGYYFVRTFYRKDK"
BASE COUNT         1017 a          245 c          478 g          668 t
ORIGIN      
        1 gcagaaaata gaaaacttta aatttttcgg tcaatatgca aatcttaaag aattgagaaa
       61 atataataat ggtgatgttt caattaatga aaatgttcct agttatgatg ttgaatacaa
      121 aatgagcaat aaagatgaaa tagttaagga attaagaagt cgttataata tttcgactga
      181 aaaatcacct atattaaaaa tgcatattga tggggacctg aaaggcagtt ctgtaggtta
      241 tagaaagtta gaaattgact tttcaaaacg tgaaaacagc aaattatcag tcattgaatt
      301 tttaagttat aaaccagcga aaaaatagta ttgataagga atattagggt gtgaaaaaat
      361 gcgatatcta aaaaagcttg catggttcat aagtgttatt attttgggca tttttataat
      421 aggttgtgat agttcaagcg atactgggga aaaagcaaaa gaagattcaa aggaagaaca
      481 aatcaaaaag agctttgcga aaacattaga tatgtatcca attaagaatc tcgaggactt
      541 atatgacaaa gaaggatatc gagatagcga atttaaaaaa ggtgacaaag ggatgtggat
      601 gatatataca gatttcgcca aaagtaataa accgggtgta ttagataatg aaggtatgat
      661 tttaaatttg gatagaaata cacgtacggc caagggatat tattttgtag atactatata
      721 tgacaatcat gaaaactctt atagtaaaaa ttatagagtt gagatgaaaa acaataaaat
      781 tattttatta gacaaggtgg aagatcaaaa acttaaagaa agaatagaaa actttaaatt
      841 tttcggacaa tatgccgatt tcaagagttt gaaaagttac aacaatggcg acgtttcaat
      901 taatagtaat gttccaagtt atgacgcgaa atttaaaatg agtaataaag atgaaaatgt
      961 taagcaatta agaagtcgtt ataacattcc tactgaaaaa gctccaatat taaaaatgca
     1021 tattgatggg gacttaaaag gcagttccgt tggatataaa aagttagaaa tagacttttc
     1081 aaaagaagaa aatagcgaat tatcagtagt cgattcatta aattttcagc ctgccaaaaa
     1141 aaataaagat gatgaatgat agggaggatg tgaaacaatg tggtcaataa aaagaattgg
     1201 aatgtacaca aatgttataa ttttgagcgt ttttataata ggttgtgata gttcaagcaa
     1261 taatgcagaa aatcaaagag aagactcaaa agaagaacaa atcaaaaaga gctttgcgaa
     1321 aacgttagat atgtacccta ttaagaatct cgaggattta tatgacaaag aaggatatcg
     1381 tgatggtgag tttgaaaaag gcgataaggg gacgtgggta ttgtattcag ctatggtatc
     1441 acaaccaaaa ggcgagagtt taaaatcacg aggaatgata ttaaagttag atagaaataa
     1501 gagaactgct aaaggaagtt atattattag agaattgaaa gaagataaaa atcatgatgt
     1561 tcaaaaaaat gaaaagaaat atccagtgaa attggtgaat aataggatag ttttgataaa
     1621 agatgttaaa gacaaaaagt taaaaaaatg aaatagagtc gtttgaatta ttttcacaat
     1681 atggaaactt taatcatttt gatcggaatg agattactaa tatttcatat aatcctaatg
     1741 ctcccaatta ctctgcagaa tataaaatga agaaaaatga cagaaacatt caacagttga
     1801 aaaagagatt taatctaaaa actagcaaga caccaaaatt attgtttaag ggatctggag
     1861 atataaaggg gtcttctgta ggatataagg aaatagaaat catatttagt agaagtaaag
     1921 aagaaagcat ttattatgtt gacagcgttg agttcgttcc aagtaacaaa ataacaaaaa
     1981 gttgatatta gtcaacaaaa gcaaatattg agaataaaga gagttgtgaa atgatgggtt
     2041 atttaaaaag acttgtattg tatatagtta ttatggttat gagtgttttt ataataggtt
     2101 gtgataaatc aagcgatact gcagaaaaat caaaagaaga ctcgaaagaa acacaaatca
     2161 aaaagagttt tgcgaaaacg ttagatatgt attcaattaa gaatctcgag gaactatatg
     2221 acaaagaagg ctatcgagat ggcgaatttg aaaaaggtga caaagggatg tggacgatat
     2281 atacagattt cgccaaaagt aataaaccgg gtgaactaag taatgaaggc atggtcttgt
     2341 acttagatag aaacacacgc acagctaagg ggtactactt tgtcaggacg ttttatcgca
     2401 aggataaa
//
LOCUS       DALAGF010000177         2376 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.184, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000177 DALAGF010000000
VERSION     DALAGF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.184"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            82..1829
                     /locus_tag="O2692_002698"
                     /pseudo
     CDS             82..1829
                     /locus_tag="O2692_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
     gene            complement(1923..>2376)
                     /locus_tag="O2692_002699"
     CDS             complement(1923..>2376)
                     /locus_tag="O2692_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCZ4300283.1"
                     /translation="MSKVDAHWELIEAIKNLRDEIAPNTLLTINDDIPDRKTGLELAE
                     KYGIDGIMIGRGIFHNPFTFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLR
                     RFFKIYVRGIRGASELRHQLMNTQSIAEVRALLDEFEAQMDEDVKIEL"
BASE COUNT          930 a          306 c          349 g          791 t
ORIGIN      
        1 ataatttaaa aactgacaat ttgcgagact cataaaatgt aataatggaa atagatgtaa
       61 aatataatta aggggtgtaa tatgaagatt aatatttata aatctattta taattttcag
      121 gaaacaaata caaatttttt agagaatcta gaatctttaa atgatgacaa ttatgaactg
      181 cttaatgata aagaacttgt tagtgattca aatgaattaa aattaattag taaagtttat
      241 atacgtaaaa aagacaaaaa actattagat tggcaattat taataaagaa tgtataccta
      301 gatactgaag aagatgacaa tttattttca gaatccggtc atcattttga tgcaatatta
      361 tttctcaaag aagatacaac attacaaaat aatgtatata ttataccttt tggacaagca
      421 tatcatgata taaataattt gattgattat gacttcggaa ttgattttgc agaaagagca
      481 atcaaaaatg aagacatagt taataaaaat gttaattttt ttcaacaaaa caggcttaaa
      541 gagattgtta attatagaag gaatagtgta gattacgtta gaccttcaga atcttatata
      601 tcagtccaag gacatccaca gaatcctcaa atttttggaa aaacaatgac ttgtggtaca
      661 agtatttcat tgcgtgtacc gaatagaaag cagcaattca tagataaaat tagtgtgata
      721 atcaaagaaa taaacgctat tattaatctt cctcaaaaaa ttagtgaatt tcctagaata
      781 gtaactttaa aagacttgaa taaaatagaa gtattagata ctttattgct aaaaaaacta
      841 tcgaattctt caactacaga aaatatatct atagatatat caagattttt agaactaagt
      901 aatatgatac tcttgttaga tgatatgctc gacgtcaata tatatatata aattctttta
      961 aaaataatac attagagaca tttgatgcga ctgatcctga agttgattac atcactgaaa
     1021 taggagatta cttattaaaa tatgacgtta attctataaa tgatgtcaga attgaagtga
     1081 tagataattt ggggcattca aataatatgc tactaaaaac gatactacat gcagaagttg
     1141 aaatggaaga tggaaagaaa tatttattac aaaatgggaa atggggttat tttaatagag
     1201 aattttttga ccttttgaat gatcatttga atgagataga aattaggtat aacacactaa
     1261 ctcccacagg tttagtattt aaagaaggag aagaaggata tataaaagaa atagtaggaa
     1321 gattgccaga agaatattta atgttacata aaaaattcat aaaaccaata aataagaatt
     1381 ttatagtaaa aggaaatgga atagagttgg cagacttata caatattaaa aacaaagagc
     1441 ttttcacgat taaaagagga attaatacat ctttatctct ttatagtcta gaacagaata
     1501 taatagcaat taacgcctta aaatatccag aatcatataa ttttgaagaa ttaatagaag
     1561 ctattcctga taactcggaa aatatattta atgatataca acggagtaca aattttagta
     1621 tagtatggat tttaccgata tcatctattg ataatatgcc cataaaagat atggttcata
     1681 ctagtaacgt aataaataaa aactttcaat taactaattt aggttcagta ttacttaaaa
     1741 ataaattagt agagtggtct ctctatttaa aagatcaaag aattaatcca attatttata
     1801 tggaaacgcc aactgaagat agaaattaaa cttttatttt aaatcaccct agttcatacc
     1861 taaaagactt tcacacaaac aaaggaggaa acttaaattc ctcctttccc ttattactca
     1921 tactataatt caattttaac gtcttcgtcc atttgggctt caaattcatc tagtagtgct
     1981 cgtacttctg caattgattg tgtgttcatc aattgatggc gaagttcgct agcgcctctt
     2041 atgccacgca catagatttt aaagaatcta cgcaaactct tgaattgtcg tatttcatct
     2101 ttctcatatt tgttaaacaa tgatagatgc aatctcaaca aatctaatag ttctttgctt
     2161 gtgtgttcgc gtggttcttt ttcaaaagtg aatggattgt ggaaaatgcc tctaccaatc
     2221 atgatgccat caataccata tttttctgca agttcaagtc ctgtttttct atcgggaata
     2281 tcatcgttaa ttgttaacaa tgtgtttggt gcaatttcgt cacgtaaatt tttaatagct
     2341 tcgattaatt cccaatgtgc atctacttta ctcatg
//
LOCUS       DALAGF010000178         2350 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.185, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000178 DALAGF010000000
VERSION     DALAGF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.185"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            147..563
                     /locus_tag="O2692_002700"
     CDS             147..563
                     /locus_tag="O2692_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCZ4300284.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     VRDLLQYKIDNYDNHIAKINASDNYDDNVERLWERK"
     gene            633..1481
                     /locus_tag="O2692_002701"
     CDS             633..1481
                     /locus_tag="O2692_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCZ4300285.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            1701..1826
                     /locus_tag="O2692_002702"
     CDS             1701..1826
                     /locus_tag="O2692_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300286.1"
                     /translation="MRYIHFSIITLITGIIMHITMYFVPFESRKMPLFMTIIFTI"
     gene            2088..2350
                     /locus_tag="O2692_002703"
                     /pseudo
     CDS             2088..2350
                     /locus_tag="O2692_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          939 a          289 c          406 g          716 t
ORIGIN      
        1 agtaattaaa aacgatgaaa tgtaaaatgt taaaaaagag acaaatttaa atttgagtaa
       61 taaaataaga agtattcaaa cgaagatgac aatatataaa gcaacattac tttagacgac
      121 caaacataac tgaaaggata gtcgcaatga aaactaaaga agtcgtagcg ctcatgaata
      181 tatctcaaga cactttaaga tattatgaaa aggttggtgt gattccacca gttaatcgag
      241 atgaaaatgg atatagaata tataatgata gtgatttaaa ttggatttat ttagtgaaaa
      301 atttgcgaaa tgcaggcgtc agtattgaat cgcttatcga attttgcagg ttagcgcagc
      361 ttcctaaaaa tgaaaatatt caagcacagc aaaagcaaat tttaaataag caactcgaag
      421 aattaaatga aaacttaaag acaattcatg atgtgagaga tttactacaa tataaaattg
      481 ataattatga taatcatatt gctaaaatca atgctagtga taattatgat gacaatgttg
      541 aacgtctttg ggagagaaag taaacctatg gcttgctata gagttcgctc catggtttat
      601 agtggacata caagataaag gaggttcatt ttatgaatca tattgaaata agtaaagatg
      661 taaagatacc agtattaggt ttcggagtat ttcaaattcc tcaagaacaa acagcagaag
      721 cggttaaaga agcaattaaa gcgggttata gacatattga tacagcacaa agttatttaa
      781 atgaaactga agtaggtcaa ggtattgaag cttcaggtat tgatagaagt gaactattca
      841 ttacgactaa agtatggatt gaaaatgtta actatgaaga tactatcaaa tcaatcgaac
      901 gatcattaca acgattaaat ttggactatc ttgatctagt gcttattcac caaccttaca
      961 atgacgtata tggctcatgg cgtgcattag aagagttaaa agaaaatggt aaaattaaag
     1021 caatcggtgt atctaacttc ggcgttgatc gaattgtaga tttaggtatt cacaatcaaa
     1081 ttcaaccaca agtgaaccaa atagaaatta atccattcca tcaacaagaa gaacaagttg
     1141 cagcacttca acaagaaaat gttgtggtag aagcttgggc accatttgca gaaggtaaaa
     1201 atcaattatt ccaaaatcaa ttgttgcagg ccattgcaga taaatacaac aaatcaattg
     1261 cacaagttat tttacgctgg ttagtagaac gtgacattgt tgtactcgca aaatcagtga
     1321 atccagaacg tatggctcaa aacttagata tatttgattt tgaacttaca gaagaagata
     1381 aacaacaaat tgcgacatta gaagaaagta atagccaatt tttctcacat gctgatccag
     1441 agatgattaa agcacttaca agcagagaat tagatgttta atcataatat aacaaagtaa
     1501 atcatttaca ttacactaac ctgaaacttg gatgttaaag tctatgtttc aggttatttt
     1561 ttactcttaa aaagtgaaag gagcaagcaa ataagttatt atatattttt atattatgta
     1621 ataacgtaac taaggttata attagataat taatatgtct tggaattaat tatttaataa
     1681 gttgtatagc aggtggcgat atgagatata tacacttttc tattattaca ttaattacag
     1741 gtatcattat gcatattaca atgtactttg taccatttga atctcggaaa atgccactat
     1801 ttatgacgat aatttttaca atctaaaagc agtaaagact cataatataa ccaaaaacat
     1861 taataaaagt ttgttaaatg ataaggggat gagtattggt gaatttactc aaaaggttta
     1921 agggaaaaat cctacctgga gagattcaaa aactaaatgg actatttcca aaaataaagg
     1981 gcaatcacat ggtggttctt attggaagtt aataaataac aaaggaaaaa gaatagcttc
     2041 tttaactaaa gaaggaaaaa tcttaaggga ataaggtgtt gtaaattatg tgctttgata
     2101 taaataattt ggatataaaa aagttgaatt ttagaaaggt aaaaaacgca attcatttaa
     2161 gtttaagtgg cgataaatac cagtttttgg aggacggtaa aactatcaat aatacttatt
     2221 tttagctgta tatgataatg caataaacat ttttgaagat ttgttttcgc cttctgattc
     2281 tataaatttg gttcatgtag tatatgttta tagtgaacct tataagaaaa caaatgttat
     2341 aaaaaaattt
//
LOCUS       DALAGF010000179         2286 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.186, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000179 DALAGF010000000
VERSION     DALAGF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.186"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            111..485
                     /locus_tag="O2692_002704"
     CDS             111..485
                     /locus_tag="O2692_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCZ4300287.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
     gene            complement(736..1524)
                     /locus_tag="O2692_002705"
     CDS             complement(736..1524)
                     /locus_tag="O2692_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCZ4300288.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLKFLKSVS"
     gene            complement(1693..>2286)
                     /locus_tag="O2692_002706"
     CDS             complement(1693..>2286)
                     /locus_tag="O2692_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300289.1"
                     /translation="KKDQDYIEYKRKSPAQIILMIQRNLFALQMNPVIFFIINFILIS
                     YLYDKQYVQFQAITGMSLFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIAIIS
                     LLLIILQAFNITWGVIPITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVD
                     AMIHNPQSAQIFSLIIWILLVVLVIYFTIRLSSRTRL"
BASE COUNT          851 a          349 c          294 g          792 t
ORIGIN      
        1 atttaatgca aaattatgac taacatatct ataataaata aagattagat atcaatatat
       61 tatcgggcaa atgtatcgag caagatgcat caaataggga ggttttaaac atggcaatta
      121 caaaaatcaa tgattgcttt gagttgttat caatggtcac ttatgctgac aaattaaaaa
      181 gtttaattaa aaaggaattt tcaattagct ttgaagaatt cgctgtattg acatacatca
      241 gcgaaaacaa agagaaagaa tactatctta aagatattat taatcattta aactacaaac
      301 aaccacaagt tgttaaagca gttaaaattt tatctcaaga agattacttc gataaaaaac
      361 gtaatgagca tgatgaaaga actgtattaa ttcttgttaa tgcacaacaa cgtaaaaaaa
      421 tcgaatcatt attgagtcga gtaaataaac gaatcactga agcaaacaac gaaattgaac
      481 tataattttg tttagcgcaa tttggtgaag tttgatagat gatacattct attaaacttc
      541 ctttttttat gctcttttta cctaattgtt aagaggtttt gcactaatgg cacttaatat
      601 aaggaagata gccgcataac gagcagtata tatcaaatac atttcaaaaa aagctgattt
      661 ctatcaaata atgaatagta atcagctttt tcacattgcc taaggacttt atgccctagg
      721 tttatatatc acatattatg atacgctttt taaaaatttt aaataataat tcataaatgc
      781 ttctggttct tcaatatgcg gtgcatgtcc tgaatgttcg aagatttttt tcgtaacatt
      841 taaaaaatgc gcttccaatt taaaatgatt cttatcttta atcaaaggat catgttcccc
      901 attaattaac aaagtaggta cacatgtacg tttttttaaa tacataggtt catctttatg
      961 caaaattaat gaacgaattg ccaccgcact atcatcctca gacttaatgc tatatattct
     1021 ttgtttttga taccatttca tcgtcttctc ttgatttcta aacaatttag aaaataaaag
     1081 aatatacgct tcagacttat taaaacccgc tatatcatct tgatattgta ttaacaattg
     1141 agtaaagctg tgtattaagt catctttctt cgatgcaatg gtagttaatg attttacacg
     1201 ataagcatat ttatctgtaa ataacttagc aactacccca cctaaatcat gtcctagaat
     1261 atgtgcctca tgaatattta acttctccat tagaattttt aaatcctcaa cgtgatcgtt
     1321 taaatcgtat gattcacttt tagaagactt gccatgacct cttaaatcgt aagtaagtac
     1381 tttatatgat gatgctagtt gatgttgcaa atcttcaaat ccagctaaat taccatccaa
     1441 tccatgaagt aatattatag gatccccttt accttcaatt tgataagtta attggatttg
     1501 aggattaatt gtgactttat tcattgtgac acctccctca ataaactata tacccaacct
     1561 tcattctata tacgcttcca ttttataaac aattttttta aattatttat aaattaccaa
     1621 aagttattcc aacaataaaa aaactattca tctcaattaa tacaattaat caagtgaata
     1681 gttttatcat atttataatc ttgtacgtga agataatcta attgtgaaat atataactag
     1741 aactactaat aatatccata taattaatga aaaaatctgg gctgattgtg gattatgaat
     1801 cattgcatcg acggtttttt gacaaaataa taacccgatt cccgaaaact caagtcgctt
     1861 ataaaatatg ccggcaatta ctaaaataat accaatgaaa aagaaaaatt gatgtccaaa
     1921 atttgtaatt ggtataacgc cccaagtaat attaaatgct tgtaatataa ttaataacaa
     1981 ggatatgata gcgataattg tacccataat catttctatt ttattactac gcaagtaatt
     2041 cttttgagac acccttaggt atacaacaat agtcattgga aaaatcacta aacaataaaa
     2101 tagactcatt ccagtaatag cttgaaactg aacatactgt ttatcatata aataggatat
     2161 gagaatgaag tttataataa agaaaataac tggattcatt tgtaaagcaa acaaattcct
     2221 ttgaatcatt aatatgattt gtgctggaga ttttctttta tattcaatat aatcttgatc
     2281 tttctt
//
LOCUS       DALAGF010000180         2271 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.187, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000180 DALAGF010000000
VERSION     DALAGF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.187"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..458
                     /locus_tag="O2692_002707"
     CDS             <1..458
                     /locus_tag="O2692_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCZ4300290.1"
                     /translation="MLDYSWIDDVKNYKFFNSKSNILFIIEGVLMYFDESVMTQLLDT
                     IIKKMGDHNLTFAIEFCSKTIANNTKRHQSVSKLSSQPVFKYGYNDLKKLNEILPNTI
                     RVIHEYNYFDYYKNRWGLFGYCRFIPYLKKRLNNKIVLMKYKVPKRQRH"
     gene            complement(830..2053)
                     /locus_tag="O2692_002708"
     CDS             complement(830..2053)
                     /locus_tag="O2692_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCZ4300291.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLTDLFDKYDIKS
                     EILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWSYPPFQLTEKDGKLYGRG
                     TTDMKGGLMALVVSLIELKEQNELPHGTIRLLATAGEEKEQEGAKLLADKGYLDDVDS
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSE
                     LKQQDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIQYLKEK"
BASE COUNT          755 a          352 c          339 g          825 t
ORIGIN      
        1 ctatgctaga ttacagttgg attgatgatg tcaaaaatta taaatttttt aatagtaagt
       61 caaatatatt gtttattatt gaaggtgtat tgatgtattt tgatgagagt gtaatgactc
      121 aattattgga cactattatc aaaaagatgg gagatcataa tttgacattt gcgattgaat
      181 tttgctcaaa aacaattgcg aataatacaa agagacatca atcggtatca aagttatcct
      241 cacaacctgt ttttaaatat ggttacaatg atttaaaaaa attgaatgaa attttaccaa
      301 atacaataag agtaatacat gaatataatt attttgacta ttataagaat cgatggggct
      361 tatttggata ttgtagattt ataccttatt taaaaaaaag gttaaacaat aaaatagtac
      421 ttatgaaata taaagttcca aagagacaga gacattaagt tcttagacaa tgtaaaaaag
      481 ctgatttcta taaattattt gatagaaatc agcttttttc aaatgtattt gataatttac
      541 agctcgttga gctgctgttt tctagggatt tatgtcccat tccttttacg agaaggtttt
      601 tacgagtttt atgtaactat tagaagctat ctttttatat gaccattagt caatatatgt
      661 atgacaaatc agcacaataa tttcagtttt acatcactta atcaatgaaa atgtgcttgt
      721 tgatatttga atatttacat tcaaacaata ttcatcgcta tttctatcaa cttaaaattc
      781 gcaataaaaa actgccagca cgcgaatagg gcagctgaca gcatcgttct tatttttctt
      841 ttaaatattg aatggaagcc tctttaaaaa tatcaatata tttcagatac atatcttttt
      901 caatatattc atcgatttga tgtgccatta atggattacc tggtccaaaa atggctaaat
      961 caacattgtc cttattatct cctaagaaac tagaggcatc tgttgcacct acaagcgctg
     1021 aaacaaatat ttcgtcttgt tctacataac tagaagctac atctttaatc gtagtaatta
     1081 atttgctatt tttatcgctt gttacaggtc ggtgattgct tggaatatcg agtgaaagct
     1141 tattgctatc cacatcatta atgatatttt ggaaaaacga ttctataaag tcgttatcat
     1201 actcaggaac tggtcttacg ttaaattcaa gtgaagcttc atctggtaca gagttaaatt
     1261 gtttgccgcc atttataatc gaacatacag ctgtaagacc agatgcataa tttgcatcct
     1321 cttcagaaat ttcttttcca atcaatgatt tgaacatagg cgcaacatct aattcatgtt
     1381 tagtatcttg ttgtttaagc tctgaatatt tttctttaaa tagattataa aattcaagca
     1441 gtgtatcaat tgcattgtca ccaataaatg gaactgagct atggacagct ttaccagttg
     1501 cagttacttt acatgacata gaccccttat gtgcataata aattccagat ccagttggtt
     1561 cagcaataat taagctatcg acatcgtcta aatagccttt atcagctaat aatttggcac
     1621 cttcttgttc tttctcttcg ccagcagtag ccagtaatct aatcgttcca tgaggcaatt
     1681 cattttgttc ttttaattcg attagagata cgaccaaagc cattaaaccg cctttcatat
     1741 ctgtagtgcc tcggccatat aatttgccat ctttttctgt cagttgaaaa gggggatatg
     1801 accaattatc ttgatttcct gcatcaacaa catccatatg accactcaat gcgagtatag
     1861 gtgagccgtt accgatttct gcaacgatat tggcgcggtg ttcattaact ttcaaaattt
     1921 cagatttaat atcgtacttg tcgaataaat ctgttaaata attacaaacg tctatttcat
     1981 tattattttc agtttgtagt tcaacaatat ctgctagtaa ttgaattttt tctttttcac
     2041 taaaagttgt cattaagctc acacctttca aagtagtata tatatttata taaacataat
     2101 ttttattctt aaacataatt taaaatgcag aaagtagtgt gttatttgat ttcgttctat
     2161 gaaattaact gtctagtatc aacgatctta ttaacgctga tatcggatat gcttaatttt
     2221 atagtaaatt gtatgagaaa gatgcaggat attatttagc taataattat a
//
LOCUS       DALAGF010000181         2266 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.188, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000181 DALAGF010000000
VERSION     DALAGF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.188"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            190..552
                     /locus_tag="O2692_002709"
     CDS             190..552
                     /locus_tag="O2692_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HCZ4300292.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQITYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            566..1231
                     /gene="bshB2"
                     /locus_tag="O2692_002710"
     CDS             566..1231
                     /gene="bshB2"
                     /locus_tag="O2692_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HCZ4300293.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLANYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            1244..2122
                     /locus_tag="O2692_002711"
     CDS             1244..2122
                     /locus_tag="O2692_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HCZ4300294.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
BASE COUNT          799 a          370 c          404 g          693 t
ORIGIN      
        1 taacactttt caaattccaa caaatttttg atttttaaaa gtaaaaaact aatgctgtat
       61 gattacgtaa tcctgagtaa ttacccttca tgaaaattaa tttataaata attgagcaat
      121 cgtatacaat tatcgaatag tttttagtaa aatagaaaga taaacacatt gatagtgtaa
      181 ggagtgaacg tgttggaacc aattaaagaa caagaagtta tagatttact tgcttctttt
      241 gaacataaac ctgtgtatct gcatgttgaa acgacaaatg gggcctatgc gaaccatttc
      301 gatcaacgtg tttttaatgc tggtactttt ttaagaaaca tacaaataac atatacgcat
      361 gcacaactaa aaggtggtaa taaagaacct tatcgcattg gtcttaaact atcaaatggt
      421 ggttgggttt atgtacaagg tctaacgcat tttgaggtca atgagcatga tgaattttta
      481 attgcaggtt ttaattacga aggtcaactc gctgcagcac ttcaaattag tgagcgacca
      541 tttaatttat agaggaggca tcaacatgac tgacgaaaga catgtattag taatatttcc
      601 acatcctgat gatgaaacct tctcatctgc aggcacatta gcaaattata tccaaaaagg
      661 catacctgta acatatgcat gtttaaccct aggacaaatg ggcagaaatt taggtaatcc
      721 cccatttgca acacgcgaat cattaccaag tattcgtgag cgtgaactag aagaagcttg
      781 taaagttatc ggtattactg atttaagaaa aatgggttta cgtgacaaaa ctgtcgaatt
      841 tgaaccctat gaacatatag atggtatgat taaatcttta atcgatgata caaatccatc
      901 attgattatt tcattttatc ctggatatgc agtacatcct gatcacgaag caacagcaga
      961 cgctgtaatt cgaacggttg aacgcatgcc taaagaagaa cgccctcgtt taactttagt
     1021 tgcgttcagt aatgatgcaa ccgaagctct tggagaacct gatattcaaa atgatattac
     1081 agattttaaa gaacttaaaa ttaaggcatt tgaagcacat gcttctcaaa caggtccatt
     1141 tttaaaacaa ttagcaagtc ctgaaatcga tggtaaacaa cacagctttt taacagtcga
     1201 accatattgg acatatcact ttaaatctta acggaggaaa aaaatgactg aatttgattt
     1261 atcgacacga gaaggtcgtt ggaaacactt tggttccgta gatcctattg aaggcacgaa
     1321 acctactact aagaacgaaa tgacagattt acaaagtaca cataaagatt ttttatttga
     1381 aatagaagaa gttggtatta aaaacttagt gtaccctgta cttgttgacc agtatcaaac
     1441 agctggcaca tttagttttt caacaagttt aacaaaagac gaaaaaggta ttaatatgag
     1501 ccgtataatc gaaagtgttg aaaagcacta tgataatggt attgaacttg aatttaatac
     1561 actttatcag gtgttacgca ctttacaaac gaatatgaaa caaaatgcgg caggcgttga
     1621 tgtttctggg aaatggttct ttgatcgcta tagtccaacc acaaatatta aagcagttgg
     1681 taatgccgat gtcacttatg gattagcaat tgacggtgat aaagttacac gtaaagaact
     1741 tactattgaa gcaactgtca caacgctatg cccttgttca aaagaaataa gtgagtattc
     1801 agcgcacaac caacgtggtg ttgtcactgt taaaacatat attaataaag atcaagacat
     1861 agttgatgac tataaaaata aaattttaga tgcaatggaa gctaatgcaa gttcaattct
     1921 ataccctatt ttaaaacgtc ctgatgaaaa acgtgtgaca gagcgtgcat atgaaaatcc
     1981 acgttttgtc gaagacttaa ttcgtttaat tgccgcggac ttagttgaat ttgattggtt
     2041 agacggtttc gatatcgaat gtcgtaatga agagtctatc catcaacatg atgctttcgc
     2101 taaattaaag tatcgcaaat aatatcatat gattaattca aacgcgtaac attagttgcg
     2161 cgtttttatt ataccttcta gagttgtata tctcttttat aaatcttctc atttgatgtg
     2221 cctgagtcat aaatcaatat cctaagctct acaatgatat attggt
//
LOCUS       DALAGF010000182         2254 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.189, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000182 DALAGF010000000
VERSION     DALAGF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.189"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            437..547
                     /locus_tag="O2692_002712"
     CDS             437..547
                     /locus_tag="O2692_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300295.1"
                     /translation="MKMTIKVRKTNKKEKIEFLIGTFIIILVILGFKIMK"
     gene            975..1160
                     /locus_tag="O2692_002713"
     CDS             975..1160
                     /locus_tag="O2692_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300296.1"
                     /translation="MSEYKKKIIELIESNLTGYEISKKTGVSQYVLSQLRQGKREVDN
                     LTLNTTEKLYEYANKVL"
     gene            1672..1776
                     /locus_tag="O2692_002714"
     CDS             1672..1776
                     /locus_tag="O2692_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300297.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKNKI"
     gene            1905..2156
                     /locus_tag="O2692_002715"
     CDS             1905..2156
                     /locus_tag="O2692_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300298.1"
                     /translation="MLTLIKLKEDEQVIIYEYIPEDDISNGKGSVTFNKKDAEVIDFS
                     LSEIENEEYFMLYRNKSFSVVRDFIEKQEFPENYKIAWY"
BASE COUNT          920 a          218 c          357 g          759 t
ORIGIN      
        1 ttatgcacat aatttttcat tgacaatttt ttatattaaa ttgtgtaatt ggcatatatg
       61 atgatttgtg ataattatat tgtaatatag catttggttt tatgattcta aaattaatag
      121 atttgaaaag tatactttat ttcatgaaag tttttggtac atttatttga aaatgttttt
      181 attacaaagt tacatggcta aatgtttctt aacaaaatta atttttcgag tgtaaagaat
      241 cgtgtggtaa acataattta tatagattat aaggaggtga aaatggtcga ggaagttaaa
      301 acgataaaat agtttgaatg ttttgatttc aattctggta ggactattga atttgcaatc
      361 taataagaat gtgaggtttg atttaatatg aagtcatatt ctttgaataa aatattactt
      421 tctcaccatt agtaaaatga agatgactat taaagtgcga aaaacaaata aaaaagagaa
      481 aatagaattt ttaataggga cgtttataat tattttagta atattagggt tcaaaattat
      541 gaaataaatg tatagtattt gagaagtcta tatgactccg gaaaaattaa attctaaaga
      601 tatctcaata tctcaaattt aatagactac tggagttagt agtttgaata ttgaaagaca
      661 ctaatctaat aaaaatacat aaaaggttaa actttagaag tggctgtaaa cttcaatgaa
      721 tactaaacat tgaagttttg taaaaggcgt aaggtataca agacaaatcg tttatctttg
      781 acaaagtaat tgatgatgaa aagtcgttaa tatgtagaaa caaaaattga aaatataaaa
      841 tttaactatt atgaagtgat gatagccatt aatgaataga aaaataataa aatcatttat
      901 agataaaaaa tacgttaagt tcacagaatt tcgaaaaaaa gcagaaaaca ttgtacagat
      961 atggagatga aaaaatgtca gaatacaaga aaaaaataat tgaattaatt gaaagtaatt
     1021 taacaggata tgaaatttct aaaaaaactg gagtttctca atacgtactt tcacaattaa
     1081 gacagggcaa acgcgaagta gataatctaa ccctgaatac aacagaaaaa ttatatgaat
     1141 atgccaataa agttttgtaa tttaactaat gtataaatta atcaagctat gtttatttga
     1201 tttaactatt aataaaaatc atatggtgaa tggatattat aataattaaa atacaaaaat
     1261 agtagattcc aatttgtgaa aaaggagtat gtcgtttcat ttagaattta ttgcacgaaa
     1321 ttagcatcga ataagggaaa ataagtgtta agttttaaat gataaaaaag attggaatgg
     1381 atcgtcttga aatgctccct tcaaagtttt cattttttca atgtcgactt cgaagggggc
     1441 attttcatta aattgttata gctttttata tttgtataat gaacatataa gtttaagaag
     1501 gtgcgagtga aggaaataaa aaagctcaaa tgtaccaaat tgttaatctt aataaatctc
     1561 tactttataa agattgaatg gacattcgag cgttaatcag tcaggaggga ctttccctcc
     1621 tacaatttaa taataatact tgcttcacca ctatacaagg agtgagttgt tatgttcaaa
     1681 gtgaattatt cgattttaag ttattatcca gaatataata tcgcagtaag ttggcaacgt
     1741 ttaagagaag gaaaaacaat aaaaaacaag atttaatact gctgcgtcat gaggcgcttg
     1801 aacattattt gatgaataag tataatttca actatgatta tgcacataaa attgtatcaa
     1861 aaaaatacga ttattcaatt tttataaaaa agaaggtgga ttaaatgctt acattaataa
     1921 aattgaaaga agatgaacag gttataatat atgaatatat acctgaagat gatataagta
     1981 acggtaaagg ttcagtaact tttaataaaa aagatgcaga ggttatagat ttctcattat
     2041 ctgaaataga aaatgaagaa tattttatgt tatatcgtaa taagtctttt tctgtagtaa
     2101 gagactttat cgagaagcaa gaatttcccg aaaattataa aatagcatgg tattaaagca
     2161 tcctttctac acagataaag agaaagcgat gctattttta tatgcttttt tcattattcg
     2221 ttgaaaaaga agagtcacaa aagttaacat attt
//
LOCUS       DALAGF010000183         2229 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.190, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000183 DALAGF010000000
VERSION     DALAGF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.190"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            17..583
                     /locus_tag="O2692_002716"
     CDS             17..583
                     /locus_tag="O2692_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCZ4300299.1"
                     /translation="MNTKFLGKTLVASALVLTTLGTGLHSSYLGLDTNKVVKTAKAEE
                     KMTDGQLWKKVKDSLHDSDIILSNEYETINVTYLLSNGYSSSVSAPGNDDGGHLTQSI
                     DFKGLKQIDLTKENVYDDFNKKLDAKNTWNSLTEKLKGLGLLQNGQKVSIYSSDSSSP
                     VSGKVGEGVTSGGENTLTKRFINKITID"
     gene            complement(1057..1755)
                     /locus_tag="O2692_002717"
     CDS             complement(1057..1755)
                     /locus_tag="O2692_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
                     /protein_id="HCZ4300300.1"
                     /translation="MIINELKSCKLKFSKQALTFVPIIVTILFILFINWYLNVNLWNG
                     RQISLFTASFNAITSLLISINVYQVINFEENIGHFNHILGKANRLNWLNASMIFTYTI
                     TAICILLASINLLWHSHDMKITLMFIGVSLFFNVIILLLLFIFSIFIKDVMAIVVGVL
                     MFIFNVYFGLEVLGDHSWFYLPITYATRYVYMFIEGSIPVTLTLAIYIIITLLLAVLL
                     FKGFNKWSGRTIKD"
     gene            complement(1752..>2229)
                     /locus_tag="O2692_002718"
     CDS             complement(1752..>2229)
                     /locus_tag="O2692_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HCZ4300301.1"
                     /translation="TKEIRAKIFILSIAFLVHCFLVFIIAYIGDLIINPHPVNAMLLL
                     VTILLMYVVSLPLIPLNFLLTRYFGVFVSILINLVLSVICVLFLTLKSLFWVLPWGIM
                     QRIPLITLGILPNGLVVNHNSKYFNDLNALYISIIVSIIIFAIVTFLNNKKSWRLK"
BASE COUNT          874 a          366 c          292 g          697 t
ORIGIN      
        1 aaaaggagca ataaaaatga atacaaaatt tttaggtaaa acattagtag caagtgcttt
       61 agtattaaca acattaggaa caggcttaca ttcttcatac ttaggattag atacaaataa
      121 agttgttaaa acggctaaag cagaagaaaa aatgacagat ggtcaattat ggaaaaaagt
      181 taaagattca ttacatgatt ctgatattat tttaagtaac gagtacgaaa caatcaacgt
      241 tacttattta ttaagcaatg gatattctag cagcgtttct gctcctggaa atgatgatgg
      301 aggccatcta acacaatcta tagactttaa aggattaaaa caaattgatt taactaagga
      361 aaatgtctac gatgacttta acaaaaagct agatgctaaa aatacatgga attctctaac
      421 cgagaagtta aaaggtcttg gtttactaca aaatggccaa aaagtatcta tttattcttc
      481 agatagctct tctccagtct cgggtaaagt gggagaagga gtaactagtg gtggcgaaaa
      541 tactctaact aaacgcttca tcaacaaaat cacaattgat taaaccttaa actcaaattt
      601 ccttaattca taaaacatta ataactaacc ctttcttaca aaccacctag cccataatat
      661 attaaaacgt catttttatt tcaattagcg gaaaataaaa aaaccattcc gcattaactt
      721 tggcaagttg gaatggttaa aaaaatattt tagaagccag cgtttggctt gcttagacga
      781 catgttcgcg catgtcgtct tatttttaca tttatataat aacacgcaat tcaataaaat
      841 gtctaatcaa taaacctcaa ttacattcaa attcaaaaac ttttcaccta atattcatcg
      901 cttaaataaa cattacgttt aattaaacag acattgtcaa tcgcgtctaa tacccctgct
      961 gtccgaaaac aaaattattt tgtattcata tttaactgtt ctctttcctc ccttttcaac
     1021 gcaactaaat ttcttttacc gttagagccc caatctttaa tctttaattg ttctaccgct
     1081 ccatttatta aatcctttaa agagtaaaac tgctaatagc aacgtgataa taatatagat
     1141 tgccaatgtt aatgtaactg gtatactccc ttcgataaac atataaacgt aacgtgtagc
     1201 atatgtgatt ggtaaataga accacgaatg atctccaagc acttctaatc caaaataaac
     1261 gttaaaaata aacattaaaa ctccgacaac aatagccatt acatctttaa tgaaaatact
     1321 aaaaataaaa agtagcagta atataattac attgaaaaac aatgatacgc ctataaacat
     1381 aagtgttatt ttcatatcat gtgaatgcca caataaatta attgatgcta atagaataca
     1441 tatggctgta atagtataag taaaaatcat tgatgcattt aaccaattta gcctattagc
     1501 ttttcctaaa atatgattaa agtgaccaat attttcttca aaattgataa cttgatagac
     1561 gtttatagaa attaatagcg atgtaattgc attaaaactc gctgtaaaca aacttatttg
     1621 tcgaccattc cataaattta cgtttaaata ccaatttata aataatataa acaatatggt
     1681 tacaataatg ggtacaaatg tgagcgcttg cttagaaaat ttcaacttac atgactttaa
     1741 ttcgttaatt atcattttaa tcgccaactt ttcttattat ttaaaaatgt tactatcgcg
     1801 aaaataatga tgctaacaat aatcgaaata tataaggcat tgagatcatt aaagtatttt
     1861 gaattatggt ttacaactaa accattaggt agtataccaa gcgtaataag cgggattctc
     1921 tgcattatcc cccacggcaa cacccaaaat aaactcttca atgttaaaaa caagacacaa
     1981 atgactgata atactaagtt tattaatatt gatacgaaca caccaaagta tcgagttaat
     2041 aaaaagttga gcggtatcaa tggtagagat actacataca tcaacaatat tgtcaccaat
     2101 aacaacatag cattaaccgg atgtggatta ataattaggt cacctatata agcaataata
     2161 aatactaaaa agcaatgtac caaaaatgct attgataaaa tgaaaatctt tgctcttatt
     2221 tcttttgta
//
LOCUS       DALAGF010000184         2171 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.191, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000184 DALAGF010000000
VERSION     DALAGF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.191"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..294)
                     /locus_tag="O2692_002719"
     CDS             complement(<1..294)
                     /locus_tag="O2692_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ4300302.1"
                     /translation="MLCETENINKNPKYRIIKYKDEYLMIDLVSTWLALFFPMINWLI
                     PKKYVKISEKDFETLNIVKTAKINSFWPVAGSTVLFGVMLRRYSHLFIVKYEYS"
     gene            complement(418..1047)
                     /locus_tag="O2692_002720"
     CDS             complement(418..1047)
                     /locus_tag="O2692_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCZ4300303.1"
                     /translation="MLLCDVRVIYKNPKYKVIQHNGEYLLVDLVSTWFVYFFPFINWF
                     IPKKYAIISEEEFENLNVVKPNKNNVFWSVIGSSVLFGVTLRKYIHVFDVQLDKLVVM
                     ILCALALICVIVFYFNLNRKLKLKVFDTNIEKNKRVILIPTFKLGCFLVFGYIFAGSF
                     SIFSLIALMTIEPQNIIIFIYWIMMTMLFFLLNMTSIGNEKVRVIMKNN"
     gene            complement(1500..2129)
                     /locus_tag="O2692_002721"
                     /pseudo
     CDS             complement(1500..2129)
                     /locus_tag="O2692_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
BASE COUNT          797 a          365 c          232 g          777 t
ORIGIN      
        1 actatattca tatttaacga taaataaatg ggaatacctt cttaacataa caccaaataa
       61 gaccgtactt cctgccactg gccaaaaaga attgatttta gctgtcttca caatgtttaa
      121 agtttcaaaa tctttttcgc tgattttgac gtactttttt ggaatcagcc aattaatcat
      181 tgggaaaaag agtgctaacc atgtacttac taaatcaatc atcaaatatt catctttgta
      241 tttgataatt ctatatttgg gattcttatt aatattttca gtttcacaaa gcaatgtctc
      301 cacttctttt caattggttt tatattgata cactttcata tacattagaa gtcatttaaa
      361 ttctactagc gtcttaaaag gattgtgtcg acaacattta gaatatttta aatgtaatca
      421 attatttttc attataacgc gaactttttc attacctatc gaagtcatat ttaacaaaaa
      481 gaaaagcatt gtcatcataa tccaataaat aaatattatt atattttgag gttcgattgt
      541 cataagggca attaatgaaa atattgaaaa acttccagcg aaaatatatc cgaaaactaa
      601 aaaacagcca agtttaaacg ttggtattaa tataactctc ttattttttt caatatttgt
      661 atcaaacact tttaacttaa gctttctatt taagttaaaa taaaaaacta taacacaaat
      721 taaagcgaga gcacacaata tcattacaac tagcttatct aattgaacat caaaaacatg
      781 tatgtatttc cttaaagtaa ctccaaacaa aaccgaactt cctataactg accagaaaac
      841 attattttta tttggtttaa caacatttaa attttcaaat tcttcttcgc taattatcgc
      901 gtactttttt ggaatgaacc aattaatgaa aggaaaaaag tacacgaacc aagtgcttac
      961 taaatcgact aataagtatt caccgttatg ttgaatgact ttgtatttcg gatttttata
     1021 aatgactctg acatcgcaaa gcaacattta ccactcccgt ttcattagtt ttaaatgatc
     1081 cccaatactt tttgtacctt caccaactat cgttccaaca ctatctacag ctttttctgt
     1141 ggtattttta aacagcgtct atttaatgtc ttatccaatt agttttatct cttaatccaa
     1201 atttattagt atgaaaagtc caatttttaa gaattgtcta aacagattcc gcaacaaaac
     1261 cagctaaaac cgtccatact attggattaa aaacgccaat gttataagag ctatcataca
     1321 aaataacacg ctctatattt catttttaaa ttaaaacagt tttttccttt atcaatcaca
     1381 atgggcattt tcacttggta tattcaaaca ttactatcta ttttcctgat ttaagatatg
     1441 attatatttt tttgaaccac cgtttaaata tgcgatacta ccaatttcga tcatttttac
     1501 tactcgttta ttcttaaaat aacatgaaca tttttattac ctatcgaagc catattcact
     1561 aaaaagaaaa acatcgtgat aataacccaa gttaaaaata ttattatatt ttgtgactct
     1621 gtcgtcaaaa gaatggttaa agtaaatatt gaagcgcttc ctaaaaaaaa gtacgcaaaa
     1681 actattaaca atccttgttt gaaagtcggt attaatacaa ctctattctt attaaccacg
     1741 ttggtattaa aaatttttaa tgttaatttt ttatttagat aaaaataaaa aattaacatc
     1801 cctattaaca tgataaaaaa tacagtgatt gctagttgtt tttcaaactg aacattaaag
     1861 aagttaccgt actttctcaa tataattcca aatagaactg aacttcctgc aaccggccaa
     1921 aaaacattat ttttaacagg ctcgactata tttaaccttt aaaattcatt ttcgctaatt
     1981 tttgcgtatt ttttgggcaa aaaccaatta atcataggga aaaaataagt aatccaagta
     2041 cttactaaat cgaccatgaa atattcatta ttatatctaa taactcgata tttaggattt
     2101 ttataaattt gtctagattc acaaagcact gtttaccacc cctaattaac aattgaaaac
     2161 taactaaatt t
//
LOCUS       DALAGF010000185         2153 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.192, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000185 DALAGF010000000
VERSION     DALAGF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.192"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            188..544
                     /gene="sarT"
                     /locus_tag="O2692_002722"
     CDS             188..544
                     /gene="sarT"
                     /locus_tag="O2692_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarT"
                     /protein_id="HCZ4300304.1"
                     /translation="MNDLKSKSNIKLMKRVLTTYELRKYLKKYFCLTLDNYLVLAYLD
                     VFKNDEGKYFMRDIISYIGIDQSRIVKSVKDLSKKGYLNKCRDPHDSRNVIIVVSVKQ
                     HNYIKNLLSEININET"
     gene            850..>2153
                     /gene="sasG"
                     /locus_tag="O2692_002723"
     CDS             850..>2153
                     /gene="sasG"
                     /locus_tag="O2692_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
                     /protein_id="HCZ4300305.1"
                     /translation="MRDKKGPVNKRVDFLSNKLNKYSIRKFTVGTASILIGSLMYLGT
                     QQEAEAAENNIENPTTLKDNVQSKEVKIEEVTNKDTAPQGVEAKSEVTSNKDTIEHEP
                     SVKAEDISKKEDTPKEVADVAEVQPKSSVTHNAETPKVRKARSVDEGSFDITRDSKNV
                     VESTPITIQGKEHFEGYGSVDIQKKPTDLGVSEVTRFNVGNESNGLIGALQLKNKIDF
                     SKDFNFKVRVANNHQSNTTGADGWGFLFSKGNAEEYLTNGGILGDKGLVNSGGFKIDT
                     GYIYTSSMDKTEKQAGQGYRGYGAFVKNDSSGNSQMVGENIDKSKTNFLNYADNSTNT
                     SDGKFHGQRLNDVILTYVASTGKMRAEYAGKTWETSITDLGLSKNQAYNFLITSSQRW
                     GLNQGINANGWMRTDLKGSEFTFTPEAPKTITELEKKLKRFHS"
BASE COUNT          871 a          250 c          390 g          642 t
ORIGIN      
        1 cacagaataa caaatgacaa attcatttat tatccaaaaa taaaaacaat tgaacaaaat
       61 caaaatatag tgttatgatt catttgtgtt tatagctagc accacaaact aaatatattt
      121 ggagttatta ctagttaaag acaaatatta tttgacgtga caattaaaag taagggatga
      181 agacttgatg aatgatttga aaagcaagag caatattaaa ttaatgaaaa gagtattaac
      241 tacatatgag ctgcgtaaat atttaaaaaa atatttttgt cttacattag ataattattt
      301 agtattagca tatttagatg tatttaaaaa tgatgaaggt aaatatttta tgagagacat
      361 tataagttat atcggtatag accaatctag aattgttaaa agcgtaaaag atttatcaaa
      421 aaagggttac ttgaataaat gtagagaccc acatgatagt agaaatgtaa tcattgttgt
      481 ttctgtaaaa caacacaatt atattaaaaa tcttttgagt gaaataaata ttaatgaaac
      541 ataaagtatg tttcgagatt ttaatatctt ggtgcagtta aatgtatttt ttaaatgcgt
      601 taaggtattt tttattttag aaattcaatg ctattgagtt gatgggtttt tccaacagat
      661 aagaatgaat tattcattga aaactaaaaa taataatttt atttgtagat tgttttaagt
      721 gcgtcaatat ataaaataat tttataaaga taagtgttat attccattgt gcaactccta
      781 ttgtatttgt aaaattacat gtaaattcac tgtaagcaaa gtggaaaata tggaaaaagg
      841 agtatgcaaa tgagagataa gaaaggaccg gtaaataaaa gagtagattt tctatcaaat
      901 aaattgaata aatattcaat aagaaaattt acagttggaa cagcatctat tttaattggc
      961 tcactaatgt atttgggaac tcaacaagag gcagaagcag ctgaaaacaa tattgagaat
     1021 ccaactacat taaaagataa tgtccaatca aaagaagtga agattgaaga agtaacaaac
     1081 aaagacactg caccacaggg tgtagaagct aaatctgaag taacttcaaa caaagacaca
     1141 atcgaacatg aaccatcagt aaaagctgaa gatatatcaa aaaaggagga tacaccaaaa
     1201 gaagtagctg atgttgctga agttcagccg aaatcgtcag tcactcataa cgcagagaca
     1261 cctaaggtta gaaaagctcg ttctgttgat gaaggctctt ttgatattac aagagattct
     1321 aaaaatgtag ttgaatctac cccaattaca attcaaggta aagaacattt tgaaggttac
     1381 ggaagtgttg atatacaaaa aaaaccaaca gatttagggg tatcagaggt aaccaggttt
     1441 aatgttggta atgaaagtaa tggtttgata ggagctttac aattaaaaaa taaaatagat
     1501 tttagtaagg atttcaattt taaagttaga gtggcaaata accatcaatc aaataccaca
     1561 ggtgctgatg gttgggggtt cttatttagt aaaggaaatg cagaagaata tttaactaat
     1621 ggtggaatcc ttggggataa aggtctggta aattcaggcg gatttaaaat tgatactgga
     1681 tacatttata caagttccat ggacaaaact gaaaagcaag ctggacaagg ttatagagga
     1741 tacggagctt ttgtgaaaaa tgacagttct ggtaattcac aaatggttgg agaaaatatt
     1801 gataaatcaa aaactaattt tttaaactat gcggacaatt caactaatac atcagatgga
     1861 aagtttcatg ggcaacgttt aaatgatgtc atcttaactt atgttgcttc aactggtaaa
     1921 atgagagcag aatatgctgg taaaacttgg gagacttcaa taacagattt aggtttatct
     1981 aaaaatcagg catataattt cttaattaca tctagtcaaa gatggggcct taatcaaggg
     2041 ataaatgcaa atggctggat gagaactgac ttgaaaggtt cagagtttac ttttacacca
     2101 gaagcgccaa aaacaataac agaattagaa aaaaagttga agagattcca ttc
//
LOCUS       DALAGF010000186         2153 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.193, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000186 DALAGF010000000
VERSION     DALAGF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.193"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            186..446
                     /locus_tag="O2692_002724"
     CDS             186..446
                     /locus_tag="O2692_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300306.1"
                     /translation="MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICG
                     VILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPIFYSFIG"
     gene            475..663
                     /locus_tag="O2692_002725"
     CDS             475..663
                     /locus_tag="O2692_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300307.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTSKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
     gene            851..1420
                     /locus_tag="O2692_002726"
     CDS             851..1420
                     /locus_tag="O2692_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300308.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
     gene            1508..1792
                     /locus_tag="O2692_002727"
     CDS             1508..1792
                     /locus_tag="O2692_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300309.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNKGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            1854..2072
                     /locus_tag="O2692_002728"
     CDS             1854..2072
                     /locus_tag="O2692_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300310.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
BASE COUNT          769 a          305 c          294 g          785 t
ORIGIN      
        1 tagtatgtaa atattctttt catattaata caatacaccc ctatatatgc tcacttgttt
       61 gaattaataa actttagcat tctgcaaata atattttaaa aataactata ctttttattt
      121 acctattgaa aaatagtgtt atcatattta ttaagaagct atttttatac tagtaggtgt
      181 cagtcatgaa taacattttg ttaaatgcta tcaatatagt tattactacc acttttgtta
      241 tctttaatat tttaatcaca tataataaag atttagatga tttatgttgg ctcctgcctg
      301 gtattatcat ttgtggtgtg atactcatcg tatcctttac cattgcaatg ataactaaaa
      361 actggttaag tgaaatatta ttttttataa atatcgtact cgttctctat tacatttatc
      421 ctatttttta tagttttata ggttaataat tacggtatat aaggagggtt tcacatgtct
      481 ttacattttg caattctgtt ttggctagca ttaattttct tagttgccgc tacgtttata
      541 ctcgtattaa tgaaaaaaac tagcaaagaa tctaaaaaag agtcctattt aagtttcact
      601 gtcattctct atatttttgg attcgctata ttaatataca catttatatt tggtgtgcta
      661 taaatgaaat tcattttata tccagtctga aacgtctcaa tgatgtttca gactgttttt
      721 tattcttaat agacatttca cactctcata ctttaatatt taagatatgt tttagtctta
      781 caagcttatt aaagattaca attctaattt aagactattt acatatattt cttcaacaag
      841 gagcttttaa gtgaactata ttaaacgaac gataatttta cttatattat tcgttgtcgt
      901 atccccaata aactcaccaa aaacaattgc agataataag tattcagaaa ttcaagatga
      961 caaattccaa ttacaacctg gtgatattat tgttacaaaa ggccctgtca tgtggggatt
     1021 ttttggtcat tgtagtatcg cgattgatga taaaacgatt ttacaaattg aggggcctgg
     1081 cgacaaacca actacacaat ccttcgaatc ttttaaatat aattatgcaa gtggcaaaaa
     1141 tgattggatg aaagtttatc gttgcagcta tcctggtgca ggtaaaaaag cggctgaatg
     1201 ggttaagaaa aactatgaga atacaaatca tcgttatcta gttacattaa atttgaacag
     1261 caaaaaattt acgtattgta ctaaaattat ctaccaagct tataaatttg gtgtaagtga
     1321 aaaatcagtt aaaagttatg gactacatat tatttcaccc tatgcaatta aagataattt
     1381 tatagatcca tacaaactaa ggcttgttaa agcttattaa cacagtttca tcaaatagtt
     1441 aaattagttt gtgttttgca ataaattggg tatagattac aaatgatatt tcaggaggcc
     1501 caatattttg aatgaactaa ctaaagaaca aaagtataca atagctaaat tttacaaatt
     1561 atatattgaa cgttcgaata aaggagaaac agaaacagtc gctaactttt ttggcgatgc
     1621 taaagatgca cgtgaaaact atttttgcga tcgtgattat caagacttct taactaactg
     1681 ccaaatttta attcaaaaca aatacttaac tggtgaagtt ttggacgata acatttacaa
     1741 tatttctatt ttaaacaaga catttattga attcgaacaa aattttggtt gaacatgaat
     1801 gtctcaacta taaactttct aaccctattt taaagcagga gtgaaaaaca tacatgagtg
     1861 aatggcatat taataatcaa tctacaaaac cttttttaat acaacaagcg gaaaagcaat
     1921 tgtctattgt taaaccgttg cctaatggta gcttccaaat actcacagaa ataaatttgg
     1981 agaatggcaa tattagtaac atcgatcatt ctttacttgt ttcagtagac cccaaagcat
     2041 tagaaataag tatatttgac gctgtaaatt aaacgcaata tttttattta attattttaa
     2101 tgtttcgcct tttaaataaa aacaagatta ctgaattatt atgagatttt aaa
//
LOCUS       DALAGF010000187         2111 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.194, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000187 DALAGF010000000
VERSION     DALAGF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.194"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            128..319
                     /locus_tag="O2692_002729"
     CDS             128..319
                     /locus_tag="O2692_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300311.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            complement(458..643)
                     /locus_tag="O2692_002730"
     CDS             complement(458..643)
                     /locus_tag="O2692_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300312.1"
                     /translation="MLYILITALNVILNKLKMKDSNIISFIYSRHDRILYVNLVGNRF
                     KNNISMTKATSNIGRGF"
     gene            complement(579..860)
                     /locus_tag="O2692_002731"
     CDS             complement(579..860)
                     /locus_tag="O2692_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCZ4300313.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            1149..1844
                     /locus_tag="O2692_002732"
     CDS             1149..1844
                     /locus_tag="O2692_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCZ4300314.1"
                     /translation="MTVLTDKVAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKEKLQ
                     NVANQLSQDSVKVVPTDVTNKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
BASE COUNT          737 a          314 c          382 g          678 t
ORIGIN      
        1 gtgaaataca tcacaaatcc ctttatttat ttggaaattc atgtaatatt agacttgtaa
       61 gaagttaata aatagagaga gacgagagag tttatataaa tactatataa acattggagt
      121 gatgattatg agaaaagaga ttgaagcgct tattttctca gacgtaagta gctatgatat
      181 ttacgtgaac actggtgtaa atcaaggatt aattggtgac atcaaagatg gttacctaac
      241 tattgattct atgccttaca ttgatgctga gcgtttgtat cactttgcta tggaacgtaa
      301 atcgttagtc actaactagt tcttattgcc aatgattact acccctagtc ggcggcaatt
      361 gaagtgtgat tgatgtaact tgccctcgtt ggtgagcaat tgagggcaga cccctttaat
      421 taagtaaacc ctaactcccc acaaatctgg aacgatacta aaagccacgt cctatattgg
      481 atgtggcttt agtcatactt atattatttt taaaacgatt acctacaaga tttacatata
      541 aaattctatc atgtctgcta tatataaacg aaatgatatt agaatccttc attttcaact
      601 tgtttaaaat tacgtttaat gccgttataa gaatatacaa cattatataa ttttacttgg
      661 gcgtcatgga cacttgagaa gggcattctt acatcgtcca tatttttaac ttcgtgtcga
      721 aatgcttttt cagctttttg tctttctgca agagacatac ttagccattc ttctttgcta
      781 aataaatctt cgaatcgaaa atcaaatgcg gttcttttac tttctgctct taatgctaaa
      841 acacgctcat gatatgtcac gttttattcc tccctctaat cattcaatcg aatgataaat
      901 cacagtatta actttattac taatataatg tttttacaat aaagaagtga cgatttttca
      961 acatttttaa aaataaaaaa tataagcaac gattaattgt atgtttcgaa tggcttgtaa
     1021 tgtaatttat ctatgaaaat gaagcgagtt attatgaccg ttatatgaat tgtgatacgc
     1081 tttttaaaag taatatgtat ttatgattta ccctgaaaaa ataaaaagaa tcaaaggagt
     1141 cgatcattat gacagtatta acagataaag tagcagtagt tacaggtgca ggtagtggta
     1201 ttggagaagc aattgcaaca ttactacatg aagaaggggc aaaagttgtc ttagcaggta
     1261 gaaataaaga aaaattacaa aacgtagcga atcaattgtc acaagatagt gtgaaggtag
     1321 tgccaacaga tgtaacgaat aaagaagaag tcgatgaatt gataaaaatt gcacaacaaa
     1381 cattcggtgg tttggatatt gttatcaata gtgcggggca aatgttgtcg tctaagatta
     1441 ctgattatca agtagatgag tgggatagta tgattgatgt gaatatcaaa ggcactttat
     1501 atacggcaca ggctgcatta ccaactatgt tagaacaatc aagtggccat cttattaaca
     1561 ttgcatctat ttctggcttt gaagtaacga aaagtagtac gatttatagt gcgacgaaag
     1621 cagcagttca cactattact caaggattag aaaaagagtt ggcaaagaca ggcgttaaag
     1681 taacaagcat ttctccagga atggtagata cagccataac tgccgcatac aatccaacag
     1741 atcgtaaaaa acttgaacca caagatattg cagaagcagt attatatgca ttaacacaac
     1801 caaagcacgt taacgtgaat gaaattacag tgcgtcctgt ataaaaaggt gtttgaggtc
     1861 tggggtatag gcatttaatg taatagctat cgattttgtt aatgttagta aggcaataat
     1921 tacatgaggg cataataggc aattattcct tgtacaattc tcagtaattg ctgattttaa
     1981 caatcaagtt aatgaaggcg acgatgttag tgttatggtt tataatctgt aatgttcata
     2041 attatagatt ataaaaattt tttcattttc caccacattt taggagttac tttaatgaac
     2101 aaaaaacatg t
//
LOCUS       DALAGF010000188         2058 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.195, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000188 DALAGF010000000
VERSION     DALAGF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.195"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            59..157
                     /locus_tag="O2692_002733"
                     /pseudo
     CDS             59..157
                     /locus_tag="O2692_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            250..1044
                     /locus_tag="O2692_002734"
                     /note="aph(3')-IIIa"
     CDS             250..1044
                     /locus_tag="O2692_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCZ4300315.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1320..1891
                     /locus_tag="O2692_002735"
                     /pseudo
     CDS             1320..1891
                     /locus_tag="O2692_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT          674 a          331 c          479 g          574 t
ORIGIN      
        1 cgaacactga catgatatat tagtgattag ctatattttt tactttgcaa cagaaccttg
       61 taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt tatacgccaa
      121 ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga
      181 gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg
      241 aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac
      301 cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa
      361 aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg
      421 gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg
      481 cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt
      541 tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag
      601 tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac
      661 agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa
      721 aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg
      781 gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt
      841 gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag
      901 tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat
      961 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat
     1021 attttactgg atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca
     1081 agctttttag acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt
     1141 ttatatcttt tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg
     1201 gtgaagaggt ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca
     1261 gctagcttaa gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt
     1321 tgaacaggat taacagagta acctctattc ttattcagct gcaatcaaaa aaataattcc
     1381 tgccaaagaa attgcacagc gatttaatat aagcttaagg acagtttaca gagatatccg
     1441 gacacttgaa gaagccggaa taccaattgg atctgaggcc ggaaaaggat attttcttgt
     1501 agagggcttc ctacttctgc cggtaatgtt tacagcggcg gaagtgggtg cattgattac
     1561 agcagggaaa tttttaaatt gccatggaga tgaatcattt ataaaggatt ttgattcagc
     1621 tatgtataaa atcaaatcta ttttgaagca tggtgaaaaa aactatgcac aggagttgga
     1681 gaacagtatt aacgtgtaca gcacatctgg acagaaaaat acattggcgg ataacgttat
     1741 tgcagcaatt cagactgcaa tctgcaataa aagggtaata tcaattcaat atcctgcatc
     1801 aggagggcaa gaaccggaaa gcagaatgat agagcctata tcactggggt tttatgaaca
     1861 gaactggtac ttaatcggtt ttgcaggcta agaaatgaat ataggaattt tcgtgtggat
     1921 cggattaaaa gcatttgcat agaagaagag ctctgtcaaa gccatgatgg ctctttggaa
     1981 caaatattaa aacaaatgct ggttctgttg caaagtaaaa aaatatagct aaccactaat
     2041 ttatcatgtc agtgttcg
//
LOCUS       DALAGF010000189         2029 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.196, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000189 DALAGF010000000
VERSION     DALAGF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.196"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..178
                     /locus_tag="O2692_002736"
     CDS             <1..178
                     /locus_tag="O2692_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HCZ4300316.1"
                     /translation="KLKVSFDYIDWKNTEFDQLGQENYYNYRKFGIIPEMEYEMEEVK
                     QIEQYIKEQEEAEQ"
     gene            189..684
                     /locus_tag="O2692_002737"
                     /pseudo
     CDS             189..684
                     /locus_tag="O2692_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
     gene            695..1186
                     /locus_tag="O2692_002738"
     CDS             695..1186
                     /locus_tag="O2692_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ4300317.1"
                     /translation="MTFEEKLSEMYSEIANKIISMIPVEWEKVYAMAYIDEECGEVFY
                     NYTEPSSDELFYYTSVIKKYNLLKSSFMDSVYELHDQFEELREVFIEEGLEPWTSCEF
                     DFTKEDKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPETEYEMEEVKEIEQYIKE
                     QEE"
     gene            1396..>2029
                     /locus_tag="O2692_002739"
     CDS             1396..>2029
                     /locus_tag="O2692_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCZ4300318.1"
                     /translation="MTTKENIDILRKPGAQALSLASLFMILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIIISLLQWIRFIDFEKESAQKYKKIYARFLVIINVLTTITAVFATC
                     NLYYFVAVQNHYDLFNYWLMGTISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTHF
                     GLLLTALSAFIYIERIIEYILVPNVVESKFVIMVSIIIIACTQFVAFQFIM"
BASE COUNT          796 a          209 c          367 g          657 t
ORIGIN      
        1 taaattaaaa gtttcatttg attatattga ttggaagaat acagaatttg atcaattagg
       61 ccaagaaaac tactataact atagaaaatt cggaattata ccagaaatgg aatatgaaat
      121 ggaagaggtt aaacaaatcg agcaatatat taaagagcaa gaagaagctg aacaatagag
      181 gcgataacat gattttcgaa gaaaaactaa atgaaatgta caacgagatt gcgaataaaa
      241 ttagtagcat gataccagta gaatgggaaa aggtatatac aatggcttat atagatgatg
      301 gaggaggtga agtattcttt aattatacta aacagcgatg aattgaatta ttacaccgat
      361 atacctaagg agtataacat ttctgtgcaa gtatttgatg atttatggat ggatttatat
      421 gatttgtttg aggaattaag aaatttattt aaagaagaag gactagaacc atggacatca
      481 tgcgaatttg attttacaag agaaggtaaa ttaaaagttt catttgatta tattgattgg
      541 ataaattcag aatttggtca agtaggtcga caaaattact ataagtatag aaaatttgga
      601 attttaccag aaacggaata tgaaattaat aaagttaaag aaatcgagca atatattaaa
      661 gagcaagatg aagctgaact ataggggcga taatatgact ttcgaagaaa aattaagtga
      721 aatgtatagc gagattgcga ataagattat tagcatgata ccggtagagt gggaaaaagt
      781 atatgcaatg gcatatatcg atgaagaatg tggagaagtg ttttacaatt atactgaacc
      841 aagcagtgat gaattatttt actatacaag tgtgataaag aagtataatt tattgaaatc
      901 aagctttatg gattcagtat atgagttgca tgatcaattt gaggaactaa gagaagtatt
      961 tatagaagaa ggtcttgaac catggacatc atgcgaattt gattttacaa aagaagataa
     1021 attaaaagtt tcatttgatt atattgattg gataaataca gagtttgatc aattaggccg
     1081 tgaaaattat tatatgtaca aaaaatttgg tgttttacca gaaacggaat atgaaatgga
     1141 ggaggttaaa gaaatcgagc aatatattaa agagcaagaa gaatagacaa tcttaatgta
     1201 agattaaaca ataaagcttt gtttagtctt tttagcgttt aagtaaaaaa caatagagac
     1261 cataaagttg tcatcaaata ataatataaa gataatttta ggcttttata cttttaatcg
     1321 aaaagaatct aagtacttat aactttaaat acttaatatg ctattacaaa atcaatgtat
     1381 aggagatagg taaatatgac tactaaggaa aatatagaca ttcttcgaaa gccaggtgca
     1441 caagccttaa gtttagcatc attatttatg atactttttt catgtctaac tttctttttt
     1501 ggtttagatt atgaaaggtt tccaaattat ttaaagataa cgacaattat agaattaata
     1561 attatcataa ttagtttact tcaatggatt agatttatag atttcgaaaa ggaaagcgca
     1621 cagaaatata agaaaatata tgcccgattt ttagttatta taaatgtgct aactactatc
     1681 accgcagtat ttgcaacatg taacctttat tattttgttg ctgtacaaaa tcattatgac
     1741 ctattcaatt attggttgat gggtacgatt tcaatcataa ttagttattt gttattagta
     1801 attggcggaa tgttcacgtt attaaaatta cctaaagtaa caaaacgttg gggtggtaaa
     1861 actaaaacac atttcggttt attattaacc gcgttgagcg catttatata tattgaaaga
     1921 attatcgaat atatattggt gcccaatgtc gtagaatcca agtttgtcat aatggtaagt
     1981 atcattatta ttgcttgtac acaatttgtg gcatttcaat ttattatgc
//
LOCUS       DALAGF010000190         1930 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.198, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000190 DALAGF010000000
VERSION     DALAGF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.198"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            32..>1930
                     /locus_tag="O2692_002740"
     CDS             32..>1930
                     /locus_tag="O2692_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4300319.1"
                     /translation="MAKSCLHILTNNEYATTRCQDGIVLFWPIDGEIELQKFRKSKII
                     EDDIYIINHLDVFSIKNNKKTIMLYLSSDWFAELGFTFFNYHYTAKLIKSSYNLKCLL
                     LKLTYRYLDNQPLNDADIRKLQDIIKIIAKEASMDKKIAQNQYRYAYYGDLRDELEYI
                     YQNVNQRLTLKSVADKLFVSKSNLSSQFHLLMGMGFKKYIDTLKIGKSIEILLTTDST
                     ISNISEHLGFSSSSTYSKMFKSYMDITPNEYRNLSKYNKCLMLKPEPLVGKMVQEVKE
                     IILNYIEHYKNHLTDVIHIDEDKFETPKLFQTVIQINTYTEMKLVFLEGIFKTLLNKN
                     SQVVFFIMPSILKSKNTMSEEEKFTIIKTIIESDLKIAFNINNIETTYFVEEAFMSVF
                     RQISPNELSNHNNYEVHFVFDLSLMEIRTIYRMILKLHNIMLNVKLGLNITCLFEKPS
                     VFKSLVSQIKRLKFDSLIIDNANLSSPYLMGESDELLLKNILHFKNLKQVINELDIEQ
                     EKLIFLNVENHKLLNNKERDLSNSAPLIYKTLSALYHNFDGFGLNIFDNHQAFNAMHL
                     YDKNGFKTTLGLILEKFIEYVSKPKYENSYYSIFDIENYYCLVIYDWRVIESETIMSN
                     FEDSQVYIN"
BASE COUNT          758 a          232 c          295 g          645 t
ORIGIN      
        1 gtggtactta aatatagaaa gaggtaaagt tatggcaaaa tcatgcttgc atatacttac
       61 taataatgaa tatgcgacaa cgcgttgcca agatggcata gtcttatttt ggccaattga
      121 cggggaaatc gaactacaaa aatttcgtaa aagtaaaata attgaagatg atatatatat
      181 tattaatcat ctggatgtat ttagtattaa gaataataaa aaaacgatca tgttgtattt
      241 gagtagcgat tggtttgcgg aattaggctt tactttcttt aattaccact atacagcaaa
      301 gttgattaaa tcatcctata atttgaaatg tctactatta aaattgacat atcgatacct
      361 tgataatcag cctcttaatg acgctgatat tagaaaatta caggatatta ttaaaatcat
      421 tgcaaaagaa gcaagtatgg ataaaaagat tgcacaaaat caatatcgat atgcgtatta
      481 tggtgatttg cgtgatgagc tcgaatatat ttatcaaaat gtaaatcaac gattgacatt
      541 aaaaagtgtc gctgataaat tatttgtctc aaagtcaaat ttgtcatcac aattccactt
      601 acttatgggc atgggtttta aaaaatatat tgatactttg aaaattggta aatcgattga
      661 aattctactt actactgata gtactattag caacataagt gaacatttag gttttagtag
      721 tagctccact tactctaaaa tgtttaaaag ttatatggat ataacaccga atgaatatcg
      781 taatttatca aaatataata aatgtttaat gctaaagcca gaaccactag taggcaaaat
      841 ggtgcaagaa gtaaaagaaa tcatattgaa ttatattgaa cattataaaa accacctaac
      901 tgatgttata catattgatg aagacaaatt tgaaacacct aaattgtttc aaacggttat
      961 tcaaataaat acttatacag aaatgaaatt agttttctta gaaggaatct ttaaaacctt
     1021 attgaataag aacagtcaag ttgtcttttt catcatgcca tcgattctaa aaagtaaaaa
     1081 taccatgtcc gaagaagaaa aattcacaat cattaaaaca ataattgaaa gtgatctaaa
     1141 gatagcattt aatataaata atattgaaac aacttatttt gttgaagaag cttttatgag
     1201 tgttttcaga caaatatctc caaacgaatt aagtaatcat aataattacg aagtgcattt
     1261 tgtttttgat ttatcattga tggaaattag aacaatttat cgaatgatat taaaattaca
     1321 taacatcatg ttgaatgtga aattaggatt gaacattacc tgtttatttg aaaaaccttc
     1381 agtttttaaa tcactagtat cacaaataaa gcgacttaaa ttcgattcgt taataataga
     1441 taatgcaaat ttaagtagcc cttatttgat gggggaaagt gatgagttac tattgaaaaa
     1501 tattttgcat tttaaaaatt taaaacaagt aattaatgaa ttggatattg aacaagaaaa
     1561 gctgattttt ctaaatgttg aaaatcataa actgcttaat aataaagaac gagatctaag
     1621 taatagtgct ccattaattt ataagacatt aagtgcgctg tatcacaact ttgatggctt
     1681 tggattaaac atttttgaca atcatcaagc atttaatgcg atgcatctat atgacaaaaa
     1741 tggatttaaa acaacactag gtcttatatt agaaaagttt atcgaatatg tctcgaaacc
     1801 aaaatacgaa aacagttatt attctatttt tgatatagag aattattatt gtcttgttat
     1861 ttatgattgg cgagtgatag agagcgagac aattatgagt aattttgagg atagtcaagt
     1921 ttatataaat
//
LOCUS       DALAGF010000191         1924 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.199, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000191 DALAGF010000000
VERSION     DALAGF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.199"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(548..1573)
                     /locus_tag="O2692_002741"
     CDS             complement(548..1573)
                     /locus_tag="O2692_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HCZ4300320.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
BASE COUNT          674 a          344 c          239 g          667 t
ORIGIN      
        1 agcatccttt ctacacagat aaagagaaag cggtgctatt ttttgcgctt ttataacaac
       61 taaggataca agacacataa cttcaacaat atcctcatct aaacctatcg atttcctaag
      121 ctcaatttta aacacaaagt ttatttgaaa tgatgcacac ctataaaaat cttaatcatc
      181 gaaaagtcac aaacatctcc atattaaaaa cttaaatagt gcattccatt taaactaaac
      241 atcgatattc ctaccaatat agacttagta attcgattgt taatacatta ctttcataaa
      301 ataactacaa ccatcataat acttctcaaa tgtcgtcaaa ttaaagtaaa tacagcacaa
      361 attaacattt ttaaatatat aaaataaaac aagatactca ataatctgta gtaccttgtt
      421 ttggaataat gtaattttat atcatcaacg gctacaaatg agcagtttca tatataaata
      481 aacacatcta ctcaactgat cttaattaat taataaatga tttaccagat gacatataac
      541 agcattatta gattaattct aatagtttat ttaaattttc ttcggttgtc gcccaactgg
      601 ttgcgaatct aacaacacga tgttgatcat cgtacttttc ccaaacagca aatttaactt
      661 tttgttctaa ctctgctatt ttctcgttac ttaaaataaa aaattgttga ttggttggag
      721 aatcaaagta aagacgatag cctttatttt taaacccgtc tttcatctta tttgccattt
      781 cgatagcatg tctgcttata ttaaaatata aattgtccgt aaataattct aaaaattgta
      841 tgcctgttaa ccgtcctttt gctaaaaggg caccgtgatg cttgattcga gtggtaaatt
      901 gtttcggttc attatttttc gtaaaaacaa tggcttcccc gcataatgca cctatcttcg
      961 taccacctat ataaaataca tcacaatatt tagcgatgtc tttaatagtc atatctgatt
     1021 ggtcactcat caatccatac cctaatcgtg caccatccat aaataatgga agctgatatt
     1081 gcttacatac tttgcataac tcttccaatt ctgatttaga gtataatgtg ccatattctg
     1141 taggatgaga aatatatacc attcctggga ataccatatg gtccttttta aaatcacttt
     1201 taaatgtctc catgtaagtt tcaacatctg aagcactaac ttttccttcc ttagagggta
     1261 tagtaattac tttatgtcca ctatattcaa ttgcaccgcc ctcatgcaca gcaacatgac
     1321 cagtgtctgc tgaaatgacc ccttcgtaac tttctaacat tgaattaata acaacctgat
     1381 tggtttgtgt tccacctact aaaaaacgaa ttgtagcatt tggacagtca attgtatctt
     1441 taatcttttc aattgcctga gctgtgaatt gatcaaagcc atatcccgaa gcttgtacaa
     1501 gatttgtatc tactaatcgt tttaatactt tttcatgagc accttctaaa taatcatttt
     1561 cgaatgaaat cactacattt ccccctaaaa ctaatatcaa cattttaata agataaacca
     1621 atttcaaaac tagttcgata tttaaaatgt attatgaatg gttaaagttt gtatcgcatt
     1681 atcgcgaagt tgaataaata tattatacat caaacaatac attatgaaaa ttaattaatt
     1741 ttttatcaca gcgacttttc atgctggaac acatgtatta atgattgaat tttactgtta
     1801 aaagttctat gatttaggaa atagagaaaa gaggggattt aaatggaaca aattaaactt
     1861 aaaactttta cagctgagac tttagagtta ttagaaaaaa acattaatgc ttttttaagt
     1921 tctg
//
LOCUS       DALAGF010000192         1900 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.200, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000192 DALAGF010000000
VERSION     DALAGF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.200"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            128..>1900
                     /gene="clfB"
                     /locus_tag="O2692_002742"
     CDS             128..>1900
                     /gene="clfB"
                     /locus_tag="O2692_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HCZ4300321.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTTKPMST
                     QTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDISKLSDSYYADP
                     NDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     VDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPSP
                     DPDPDSDSDSDSGSDSDSGSDSDSE"
BASE COUNT          747 a          317 c          345 g          491 t
ORIGIN      
        1 aaaattttaa gttatataaa taggaagaaa acaaatttta cgtaattttt ttcgaaaagc
       61 aattgatata attcttattt cattatacaa tttagactaa tctagaaatt gaaatggagt
      121 aatatttttg aaaaaaagaa ttgattattt gtcgaataag cagaataagt attcgattag
      181 acgttttaca gtaggtacca catcagtaat agtaggggca actatactat ttgggatagg
      241 caatcatcaa gcacaagctt cagaacaatc gaacgataca acgcaatctt cgaaaaataa
      301 tgcaagtgca gattccgaaa aaaacaatat gatagaaaca cctcaattaa atacaacggc
      361 taatgataca tctgatatta gtgcaaacac aaacagtgcg aatgtagata gcacaacaaa
      421 accaatgtct acacaaacga gcaataccac tacaacagag ccagcttcaa caaatgaaac
      481 acctcaaccg acggcaatta aaaatcaagc aactgctgca aaaatgcaag atcaaactgt
      541 tcctcaagaa gcaaattctc aagtagataa taaaacaacg aatgatgcta atagcatagc
      601 aacaaacagt gagcttaaaa attctcaaac attagattta ccacaatcat caccacaaac
      661 gatttccaat gcgcaaggaa ctagtaaacc aagtgttaga acgagagctg tacgtagttt
      721 agctgttgct gaaccggtag taaatgctgc tgatgctaaa ggtacaaatg taaatgataa
      781 agttacggca agtaatttca agttagaaaa gactacattt gaccctaatc aaagtggtaa
      841 cacatttatg gcggcaaatt ttacagtgac agataaagtg aaatcagggg attattttac
      901 agcgaagtta ccagatagtt taactggtaa tggagacgtg gattattcta attcaaataa
      961 tacgatgcca attgcagaca ttaaaagtac gaatggcgat gttgtagcta aagcaacata
     1021 tgatatcttg actaagacgt atacatttgt ctttacagat tatgtaaata ataaagaaaa
     1081 tattaacgga caattttcat tacctttatt tacagaccga gcaaaggcac ctaaatcagg
     1141 aacatatgat gcgaatatta atattgcgga tgaaatgttt aataataaaa ttacttataa
     1201 ctatagttcg ccaattgcag gaattgataa accaaatggc gcgaacattt cttctcaaat
     1261 tattggtgta gatacagctt caggtcaaaa cacatacaag caaacagtat ttgttaaccc
     1321 taagcaacga gttttaggta atacgtgggt gtatattaaa ggctaccaag ataaaatcga
     1381 agaaagtagc ggtaaagtaa gtgctacaga tacaaaactg agaatttttg aagtgaatga
     1441 tatatctaaa ttatcagata gctactatgc agatccaaat gactctaacc ttaaagaagt
     1501 aacagaccaa tttaaaaata gaatctatta tgagcatcca aatgtagcta gtattaaatt
     1561 tggtgatatt actaaaacat atgtagtatt agtagaaggg cattacgaca atacaggtaa
     1621 gaacttaaaa actcaggtta ttcaagaaaa tgttgatcct gtaacaaata gagactacag
     1681 tattttcggt tggaataatg agaatgttgt acgttatggt ggtggaagtg ctgatggtga
     1741 ttcagcagta aatccgaaag acccaactcc agggccgccg gttgacccag aaccaagtcc
     1801 agacccagaa ccggaaccaa gcccagaccc ggatccggat tcggattcag acagtgactc
     1861 aggctcagac agcgactcag gttcagatag cgactcagaa
//
LOCUS       DALAGF010000193         1820 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.201, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000193 DALAGF010000000
VERSION     DALAGF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.201"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            116..250
                     /locus_tag="O2692_002743"
     CDS             116..250
                     /locus_tag="O2692_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HCZ4300322.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
     gene            complement(847..1632)
                     /locus_tag="O2692_002744"
     CDS             complement(847..1632)
                     /locus_tag="O2692_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HCZ4300323.1"
                     /translation="MKVQIYQLPIVFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
BASE COUNT          654 a          287 c          270 g          609 t
ORIGIN      
        1 ataaaaaaaa ttacagttaa gaataaaaaa cgactagtta agaaaaattg gaaaataaat
       61 gcttttagca tgttttaata taactagatc acagagatgt gatggaaaat agttgatgag
      121 ttgtttaatt ttaagaattt ttatcttaat taaggaagga gtgatttcaa tggcacaaga
      181 tatcatttca acaatcggtg acttagtaaa atggattatc gacacagtga acaaattcac
      241 taaaaaataa gatgaataat taattacttt cattgtaaat ttgttatctt cgtatagtac
      301 taaaagtatg agttattaag ccatcccaac ttaataacca tgtaaaatta gcaagtgagt
      361 aacatttgct agtagagtta gtttccttgg actcagtgct atgtattttt cttaattatc
      421 attacagata attatttcta gcatgtaagc tatcgtaaac aacatcgatt tatcattatt
      481 tgataaataa aatttttttc ataattaata acatccccaa aaatagattg aaaaaataac
      541 tgtaaaacat tcccttaata ataagtatgg tcgtgagccc ctcccaagct cgcggccttt
      601 tttgtaatga agaagggatg agttaatcat cattatgaga cccgccgtta aaatatatga
      661 ataagtctaa tgttggaaaa ggtcaaaaaa ttaatcaatt taattaagaa aatcattcat
      721 ttgcaaaggg cgaaatgggt tcttactgag ttatctatta taaaaaaata aacatagact
      781 tatgaaaaat ctctcataaa tctatgttta gtcatgacat gtgttaaata ttatttcggg
      841 cgcttcttat ttatacaaat ctaatttaat acttttaaat acaggtatat tttcgcgttg
      901 ctgttctact tcatttaagt ttaaatctac agtcaaaata tctgcggatt catttaattc
      961 tccaactaaa tctccatttg ggtttataac tatcgaatga ccagcatatt ctgtgttacc
     1021 atcgaatcca gtgctattag ttccaatgac aaacatatta ttttcaattg cacgtgcctt
     1081 tagtaatgaa tgccaatgtt gaagacgtga cataggccat tgcgccacat aaaatgcaat
     1141 tttagcacca ctacgagcag gatatcttaa taattctgga aaacgtaaat cataacagat
     1201 aagttgggtc acataagtac cgtcagacaa ttgaaagggt tcagctacgt attcgccagc
     1261 ggttaaaaat tcatgctctc ttaacatagg aactaaatga actttgtcgt attcattaat
     1321 cagctggcca cttttattca cactaaaagc tgtattaaat atttgattgt ttctaatgtt
     1381 agaaactgac ccagctacga tatcgacttt atatttttca gctaaatgtt taataaatga
     1441 aaaactttgt cctagattat tatctgcttt ttcatttaaa tgctctaaat catagccatt
     1501 attccacatt tcaggtaaaa cgactacatc tacttcagca ttcatatttt tttcgaacca
     1561 ttgcgttatt tgagtttcat ttttagaact atctccaaaa acaatcggta attgataaat
     1621 ttggactttc ataacatcac atccttgata gatcttatat ataacttact aaaagttatg
     1681 ttgaaacgca aaaaacgagc acaagacata aaatcaaagt cctaggctct acaaagttat
     1741 attgacagta gttgatgggg ccccaacata gagaaattgg aacaccaatt tctacagaca
     1801 atgcaagttg gggtgggctc
//
LOCUS       DALAGF010000194         1789 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.202, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000194 DALAGF010000000
VERSION     DALAGF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.202"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..>1789
                     /locus_tag="O2692_002745"
     CDS             <1..>1789
                     /locus_tag="O2692_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCZ4300324.1"
                     /translation="FPQFKNNYNMIYSFINSLVWRKDGSISKHTKCYISDNVIPYDAF
                     RNNFLDVLLIKMPQKNHPLNIWALHKLLKQCNLGKRDFLWTQYISINNEKVFEIINWL
                     FSNYKKLDEETAEKYMIFLTWIFSATNNKLRDLGTKSLVKLFKTFPTKIIGLLKLFEN
                     NNDPYIVERLYASVLGATLRIDICEIHIEIANYIYEEIFDKEMVYPHILMRDYARQTI
                     EYISLSKDISNINLEKIRPPYKSNWYKKEYSNLNIDDYIKSLKNKLDSHLHFSIDKIK
                     NSMTTEYGRGTGAYGDFGRYVFGYAVRNWVKGFKSDQDLSNIALMRIFEMGYDAKLHG
                     EFDMWVNRYDNFNNSIERISKKYQWIAYYEILAKLVDKFPDVQYSGLWDDYIRDIDPT
                     LLLLEIDKESKILVPSPLPSHQSNEWVKNTKVFDETKLFLEIDIDNHRYICLSSKFNF
                     EKREKEIPFEDRDSCYFLAMGYFYNKEDSNEIIKGYENNYDRGINIPRAHSIYLYEYY
                     WSEAYKNYKEGYLTESDGKLCPAIYEYFWELDYSVKDKSISFYIPCKEIVDYFSLIQT
                     EEGVWKTKFGETICINSKLLEFDNECLLIK"
BASE COUNT          718 a          173 c          278 g          620 t
ORIGIN      
        1 tttccacagt ttaaaaacaa ttataacatg atatattctt ttataaatag tttagtatgg
       61 agaaaagatg gtagcataag taaacatact aaatgctata tatccgataa tgttattcct
      121 tatgatgcat ttagaaataa ttttttagat gtcttattaa taaaaatgcc tcaaaaaaac
      181 catccgttaa atatttgggc tttacataaa ttattgaaac aatgcaattt aggaaaaaga
      241 gattttttat ggactcaata tatatctata aataatgaaa aggtttttga aattattaat
      301 tggctattta gtaactataa gaaattagat gaagaaactg cggaaaaata tatgattttt
      361 ttaacgtgga tattttctgc aactaataat aaattaagag atctcggtac taaatcttta
      421 gtaaaattat tcaaaacatt tccaactaaa attattggtt tattaaaatt atttgaaaat
      481 aacaatgatc catatatagt cgaaagatta tatgcttctg ttttaggggc gacacttcga
      541 atcgacattt gtgaaataca tatagagatt gctaattata tttatgaaga aatatttgat
      601 aaagaaatgg tatatcctca tattttaatg agagattatg ctaggcaaac aattgaatat
      661 attagtttat caaaagatat atctaatata aatttagaaa aaattaggcc accttataaa
      721 agtaattggt ataaaaaaga atattcgaat ttaaatatag acgattacat aaaatcttta
      781 aaaaacaaat tagatagcca tttacatttt tctatcgata aaattaaaaa ctcaatgaca
      841 acggaatatg gaagaggaac tggggcatat ggcgattttg gcagatatgt atttggttat
      901 gctgtacgaa actgggtaaa gggttttaaa agtgatcaag acttaagtaa tatagcatta
      961 atgaggattt ttgaaatggg ttatgatgca aaattacatg gtgaatttga tatgtgggtt
     1021 aatcgttatg ataactttaa taattcaatt gaaagaatta gcaaaaaata tcaatggatt
     1081 gcttactatg aaattttagc taaacttgta gataaatttc ctgatgttca atattctgga
     1141 ctatgggatg attatattag agatattgat cctactttac tattacttga aattgataaa
     1201 gaatcaaaaa tactagtacc ctcaccactt ccttcgcatc aatcgaatga atgggtaaaa
     1261 aataccaaag tttttgatga aacaaaatta tttttagaaa tagatataga caatcataga
     1321 tatatatgtt taagctcaaa gtttaatttt gaaaagagag agaaagaaat accatttgaa
     1381 gatagagact catgttattt cttagcgatg ggatattttt ataataaaga agatagtaat
     1441 gaaattatca aaggttatga aaataattat gatagaggta ttaatattcc tcgagcacat
     1501 agtatatatt tatatgaata ttattggagc gaagcttata aaaattataa agagggatat
     1561 ttaactgaat cagatggtaa attatgtcca gctatttatg aatatttttg ggagttagat
     1621 tattctgtta aggacaaatc aataagtttt tatattccgt gtaaagaaat agtagattat
     1681 ttttcattaa tacaaacgga agaaggagta tggaaaacta aatttggtga aacaatatgt
     1741 attaattcta aattactcga atttgataat gaatgtttac ttattaaaa
//
LOCUS       DALAGF010000195         1699 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.203, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000195 DALAGF010000000
VERSION     DALAGF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.203"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(99..1580)
                     /gene="cls"
                     /locus_tag="O2692_002746"
     CDS             complement(99..1580)
                     /gene="cls"
                     /locus_tag="O2692_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCZ4300325.1"
                     /translation="MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
BASE COUNT          582 a          303 c          213 g          601 t
ORIGIN      
        1 acaaatgtca ttattagatt tacataatat atttatcgtt attttaattt tgggcaaaat
       61 aaaaagagcc tctataatcg agactcctta caaataaatt ataaaattgg cgaaactaat
      121 tttgctaacg attctttgaa tttaacagac agcggtctat tggcatatga ttctttggtt
      181 agttgttttg attttgtaat atcatgttca taagccaccc ttaaatcttt agcaagattt
      241 tcatcatata caaaggcatt tacttcaaaa tttaattcaa aacttctaaa gtccatattt
      301 gctgtgccca ctgatacgat ttcatcatca attaagcaca ttttagaatg tataaatcca
      361 ttttcatacg tataaatttt aacaccactt gataataagt cagaggcatt tgaaaatgtc
      421 gcccaatata ctaatggatg atctggctta catggaatca ttaaatgtac atctacacct
      481 gatttagcag caattttaat ggcatttata tatgaattat ccggaatgaa atatggtgat
      541 tgtaaatata cagatttctt tgcactcata atcatttttg tataaccgta ttcaatttga
      601 tgccagtcac tagccgggcc acttgcagct atttgaattg gtgaattgcc caatggtccg
      661 ttctttttag ggaaatactt aacatcatat tcaaattgtg gacggtgcgc ttgcgaattc
      721 cagtctaaaa taaatcgcaa ctgcagtgca tcaaccgcat ccccttgtat acgtaaatgc
      781 gtatctctcc aatatcctaa ttttcctaat cctagatatt catcaccaat gttaaatcct
      841 ccgacataac ctagttgacc atcgattacg atgatttttc tatgatttct attattcatt
      901 ctgaaattca ataacggtaa ttttgaagca aaaaatgctt caacttctcc acctaacgat
      961 ttaaaatgat caaaatttgc catcttaaca tttttagatc caacatcatc atataatatt
     1021 tttacttcta gaccttgttt caatttttct tctaaagcat gtaaaattct tttacctaaa
     1081 ccatctaaag cgaaagtata gtactctaaa tggatatatt cttttgcatt tttaatatct
     1141 tttaaaactt gatcatataa atcatttcca tcaatgaaat gatcaacttt attattttca
     1201 gttaaaaaac catcttgatc catcaaaagc atacgtacta aatcatgatg tttttgaact
     1261 tgtttgttat cagtaccata attaccttta tcaaagcttt ctatttgttg ttttaaaagt
     1321 ccatcgaaat ccgttaacac gttaccattg tttttattca atttgcgtgc cgaaacggtt
     1381 ctaccaaaaa acaagtaaag aataaaacca atcaatggta agacaaaaag tacaaatagc
     1441 catgcccaag ttgaactcgc tgtacgccta tttctttcta aaaagataat aataaaagcc
     1501 aatactaaat taatgatgaa tccaatggct aaaataatag taaataacgt tcctaaatcg
     1561 tttgaaaatg taaatcgcat agtcttttct cctataaaga aaggcacaaa aaaacatttt
     1621 gcacctttca cgtcatatta tttattcaca gataaagtta aaattgcatt gaattcttct
     1681 tcattatttg ggaatgttc
//
LOCUS       DALAGF010000196         1694 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.204, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000196 DALAGF010000000
VERSION     DALAGF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.204"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            117..296
                     /locus_tag="O2692_002747"
     CDS             117..296
                     /locus_tag="O2692_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300326.1"
                     /translation="MKFKTTKECKSNNIFKRSQEINNRESEKGCLWGISMLILLFLLI
                     LFGITACSSSIHFIN"
     gene            607..867
                     /locus_tag="O2692_002748"
     CDS             607..867
                     /locus_tag="O2692_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300327.1"
                     /translation="MCIVIYLIYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVT
                     LFILSLIIFLIFIKTKDVVHLTIFILNLLFSILYCLPILFYL"
     gene            complement(920..1270)
                     /gene="scn"
                     /locus_tag="O2692_002749"
     CDS             complement(920..1270)
                     /gene="scn"
                     /locus_tag="O2692_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HCZ4300328.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
BASE COUNT          584 a          223 c          216 g          671 t
ORIGIN      
        1 attccaattt tataataata ttgcttgaca tatcaaacta gatagtacta ttttgaatat
       61 attattataa tcaaaaattc attgtaaact tttagacaaa aggaagtaat aaaaacgtga
      121 aatttaaaac aactaaagaa tgtaaaagta ataatatctt taaaagaagt caagaaatta
      181 ataatagaga aagtgaaaag ggttgtttat ggggcattag catgttgatt ctacttttct
      241 tattgattct gtttggaata actgcttgtt catcaagcat tcactttatt aattagattt
      301 ttttacttgg aggtatcatg tgaagaacca tacaaatata attaatatct tattagttat
      361 agtcaactca ttaactcatt ttctaactct aaacacctca ttttttaata attcagcatc
      421 ggatttctgt tttatcatag gggctatatt tttcttaatc ggaatttttg ttgcaatata
      481 cggtatgaag cgagcaacat attggttaaa cttattgatt ttatttacca atatttttta
      541 ttttctacac ttctgtgtgt tacttttgtt aaaatatata ggatttaaat tatttattta
      601 tgaagggtgt gtattgttat ttatctaatt tatagtctaa ttgtctattt catctgcatt
      661 ataaattttt tcataatggc tgcaccattc ttaatgacta atgctgatta tgtatggacg
      721 cctatgacta cagttacgtt atttattttg agtttgatta ttttcttgat atttataaaa
      781 acaaaagatg tcgttcattt aacaattttc atattaaact tacttttttc aatactttat
      841 tgtttgccta tactgtttta tttatgaaca cttttatatt atctagaaaa ttaaaaccac
      901 ccgtaaaagg gtggtttttt taatatttac tttttagtgc ttcgtcaatt tcgttataaa
      961 tcttttgaag ttgatatttt gcttctgaca ttttcttaaa gtcttttgac ttaagagcat
     1021 acattgcttt ttgacctgaa atttttatag ttcgcttata ataagtgttt aaacttccag
     1081 tagctaattc attaacattt agttcatcta ataacgattt taattcatta gctaactttt
     1141 cgttttgata ttcattcgat gttggcaagc ttgtgctagc ttgtgcctca tttttatcta
     1201 gattagttac tagtggtgat gctaaaacga ttgctaaagt tcccgcaagt atagattttc
     1261 taattttcat tttttattct ccttagtaat tagtactaat ttattttaat gactgactaa
     1321 aaaaaagtac attaattatt cattaactat atagatcttc ttattaaatg aaaattaata
     1381 aattatcaaa ttgacatacc ttattgtatt tatagaaaaa caaaaaaagg taagcaccga
     1441 aatgcttacc tacttcccat aaacaatata acacatatac attgatttgg aaagcgcaaa
     1501 aataaattta atatactagc ccgaagggga gcaatacata aaaaatgaaa ggcgctcctt
     1561 gaaaacgccc aaggtaatat taacataaaa tggctactat tgcattatct aattttattt
     1621 aattaaaaca aatatatata gcacaaaaaa actagcccga aggctagctt atgaatagat
     1681 gaaaatttga acac
//
LOCUS       DALAGF010000197         1624 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.205, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000197 DALAGF010000000
VERSION     DALAGF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.205"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(366..566)
                     /locus_tag="O2692_002750"
     CDS             complement(366..566)
                     /locus_tag="O2692_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCZ4300329.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
     gene            complement(923..1609)
                     /locus_tag="O2692_002751"
     CDS             complement(923..1609)
                     /locus_tag="O2692_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCZ4300330.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFYFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
BASE COUNT          503 a          296 c          206 g          619 t
ORIGIN      
        1 aaagttgatt agttaaatta ttaattaagt agtaagatat tatgcgatgt ggtatcctat
       61 tattgttaaa ctttttgagt gtttaacatt acttcttgtt atcgccatat acagacttta
      121 attctcgtca ttattaaagt tctgtattgg cgctttttta tattcaaaat caaaagtcgg
      181 acagatgaaa agtaaaaaac ttttccatca gttcgattta ttttagaacc attatatatt
      241 gttaaatctc caactaaatt tattggacaa aaaaataacg gctagtgctt ttaccacacc
      301 aactgttatc gatttaaagc ataaaaaaac agccctatag taaggactgt ttgaataagt
      361 taaaattaca ttttaactac gtttgcagct tgctcgccac ggtcgccttc aacgatgtcg
      421 aattcaactt tttggccttc ttctaatgat ttgtatccat cttcagcgat tgctgagaag
      481 tgtacgaata cgtcgctacc atcttctctt tcgatgaaac caaaaccttt ttctgcatta
      541 aaccatttaa ctgtaccgtt attcatattg aatacctccg tcgtgctttt gcactaaata
      601 ttttgtaaca aattcataaa tgaaaaggag aatattctat ataaataact cacatttttc
      661 ttcacgctct ttattactta actttcatta tacactttta aatatagaag taaagcatta
      721 ttttattttc tgtgataata cacattgaac tatatagtaa cattccattt taatactaaa
      781 tttaaaattt ttattttcta atattaaata cacttacttt tgatactatt tactttttta
      841 attctgaatt gataataaat catatcgtga tctaaaaatt ataaaagtgc cactagcagc
      901 agtgacactt ttacaatgag cattattgac ctgaatcagc gttgtcttcg ctccaaatat
      961 ttaatttctc tttttttgct tgtgcttcac tttttcttaa aagttgttca tgtgtattgt
     1021 taggtttata aacataagca actttagcca agccttgacg aactaaagct tcgtttacca
     1081 tttttccatc agcataaata tacgctaagc cacgtccata tttatcagtt ctttgacctt
     1141 tgtcaaactc gacttcaatt ttctttgcat tttctaccat ttttttcgta aatgcacttg
     1201 cttcaggacc atatttctct acaccttttt taggatgctt tgtttcaggt gtatcaacca
     1261 ataatagtct gaatgtcatt ggttgacctt tgtacattaa tttaaccgta tcaccatcaa
     1321 tcgctttaat taatgtcgca ggttctttat gtaatttttt agttgaagtt gcactatata
     1381 ctgttggatc ttcagaacca cttctattta cgccattatc tgtttgtgat gcatttgctg
     1441 agctacttag acttgaaact acaactaaag ttaacactaa acaactagta gcgaaataga
     1501 aaaacctctt tgcgtattgc cctttcgaaa cattactgat agccatccct ataagtaata
     1561 ttgaaacgat tgccatacat atgccagcac ttaataagta ttctgtcata actaacacct
     1621 cttt
//
LOCUS       DALAGF010000198         1612 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.206, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000198 DALAGF010000000
VERSION     DALAGF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.206"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            119..421
                     /locus_tag="O2692_002752"
     CDS             119..421
                     /locus_tag="O2692_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300331.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVRTFGFI"
     gene            677..1042
                     /gene="crcB"
                     /locus_tag="O2692_002753"
     CDS             677..1042
                     /gene="crcB"
                     /locus_tag="O2692_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCZ4300332.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGDLS"
     gene            1039..1392
                     /locus_tag="O2692_002754"
     CDS             1039..1392
                     /locus_tag="O2692_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HCZ4300333.1"
                     /translation="MISIILVMIGGGFGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
BASE COUNT          513 a          249 c          230 g          620 t
ORIGIN      
        1 ttgcctatat tttttaaaaa tgactttagc aaacattgtg tctacaagtt aataaaacta
       61 ttgttttatg attgtttatg atacgatatg taaatgataa tcatccagga ggtctagcat
      121 gtctcgttca aaaaaatact tttacttatc tagcttaatg attattttaa gctttttctt
      181 taatacaaat aacgttttcc taagtggact ttttaattct tttattaaat taatactttt
      241 ctgcagtgtt attaactcaa ttgtactaat tttgtctata atttttgcag atcgttcaat
      301 taaatcacta aagcctgatg cagattggat tagaattgcg agtaaaagtt tgccttggat
      361 tattctaatt gttattttag tacatatctt ttcaattgtt cgtacattcg gttttattta
      421 aaaaagttaa tatgtcattg tagccttaat acaaaacaat acaatgtatc atgctataat
      481 gagtaaaaca atttgataac gttgttgcgt ataaaaaata ttagattttc gaaatcataa
      541 ctatgcatct aatcgctata gttatacaac aagatataac atataatgag gtttgataat
      601 gcatcgacaa tttttgtcgt cgcgttgcca aaacctcttt tttaaattca aactacttct
      661 tttcgaggtg aaccaaatgc aatatgtata tatttttatc ggtggtgctt taggcgcttt
      721 attacgttac ctcatttctt ttctgaatac tgacggaggt tttccaatcg gaacactgat
      781 agccaatttg actggtgcct ttgtaatggg attgctaaca gccttaacaa ttgcattttt
      841 ttcaaaccat ccgaccctaa aaaaagctat tacgactggt tttcttggtg ctttaacgac
      901 tttttcaaca tttcaattag aattaataca tatgtttgat catcaacaat ttataacttt
      961 actactatat gctgtaacaa gttatgtctt tggtattttg ttatgttacg tcggtataaa
     1021 actaggtggt gatttatcat gatatcaatc attttagtca tgattggcgg cggttttggc
     1081 gcaattgcta gaagtgccat tactgattat tttaatcata aatttacttc aaagttacct
     1141 atcgcaacat tgatagtaaa tctagttggt agttttttaa ttggattaac tataggctta
     1201 tcaatttcaa tctcatggtt ccctgcgttc tttgttaccg gttttttagg tggcttaaca
     1261 actttctcaa cgttagccaa agaacttaca ctaatgatga cgccaaaatt taatattaac
     1321 ctttttctca attattcact tttacaattc atcattggat ttatagcttg ttatattggc
     1381 tatcatattt aaaaataaaa tgcttcattc agcaaatagg taaattacga caccttcctg
     1441 aacgaagcat tttttaattt tcatgcaaat ttttaagcac catataatgc ctaccaaatt
     1501 tcaataatct ttgttgccgt ttaaataatg tgaatgtcaa taaattctcc aaactagtcg
     1561 aaaataaagg gagtgggaca taaatcccta aaaaaaagca gtaagataat tt
//
LOCUS       DALAGF010000199         1588 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.207, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000199 DALAGF010000000
VERSION     DALAGF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.207"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            131..1558
                     /locus_tag="O2692_002755"
     CDS             131..1558
                     /locus_tag="O2692_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCZ4300334.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
BASE COUNT          575 a          218 c          338 g          457 t
ORIGIN      
        1 tgcagcaaaa ttttaaagca ttttattttg aacatattaa aagggagcgt atcataatgg
       61 aatgtaatgt ttatatcgta tgcattacgg ataaataata tataaatcat tcttgaggag
      121 tgaaagaata atgagagact acacaaagca atacattaat ggcgaatggg tagaaagtaa
      181 tagtaatgaa acgatagaag ttataaatcc agcaaccgaa gaagtaatcg ggaaagttgc
      241 taaaggtaat aaagctgatg ttgataaagc cgtcgaggcg gcagacgatg tttatttaga
      301 gttccgtcat acatctgtga aagaaagaca agcgttatta gataaaattg taaaagaata
      361 tgaaaacaga aaagacgata ttgtacaagc tattacggat gaattaggtg ctcctttatc
      421 attatctgag cgtgtccatt atcaaatggg actaaaccat tttgttgcag cgagagacgc
      481 attagataac tacgaatttg aagaacgccg cggagatgat ttagttgtta aagaagcaat
      541 cggtgtatct ggattaatta caccgtggaa cttccctaca aaccaaacat cattaaaatt
      601 agcagcagca tttgcggctg gtagtccagt tgtacttaaa ccatctgaag aaacaccatt
      661 tgcagctgtt attttagctg agatttttga taaagtcggt gttcctaaag gtgtatttaa
      721 ccttgttaat ggtgatggtg ctggtgttgg gaatccttta tctgaacatc ctaaagtacg
      781 catgatgtca tttacaggat caggccctac tggttctaaa attatggaaa aagccgctaa
      841 agattttaaa aaggtatcat tagagcttgg tggcaaatca ccatatatcg tcctagatga
      901 cgtagatatt aaagaagcgg ctaaagcaac aacaggcaaa gttgttaata atactggtca
      961 agtatgtaca gctggtacac gtgttttagt gcctaacaaa attaaagatg cattcttagc
     1021 tgaattaaaa gaacaattta gccaagtgcg tgtcggtaat ccaagagaag atggtacaca
     1081 agtaggccct atcattagta aaaaacaatt tgatcaagta caaaattata ttaataaagg
     1141 tattgaagaa ggtgctgaat tattttatgg tggtcctggt aaaccagaag gacttgaaaa
     1201 aggatacttt gcacgtccga caatttttat taatgtagat aatcaaatga cgatagcaca
     1261 agaagaaatt tttgggccag taatgtcagt tatcacttat aacgatttag atgaagcgat
     1321 tcaaattgca aatgatacaa aatatggttt ggcaggatat gttattggta aggacaaaga
     1381 aacattgcat aaagtagctc gttctattga agcaggtaca gtagaaataa acgaagcagg
     1441 tagaaagcca gatttaccat ttggtggcta taaacaatct ggtttaggtc gtgaatgggg
     1501 cgattatggt attgaagagt tcttagaagt gaaatctata gctggatatt ttaaataaat
     1561 ttgatttttg aattaaaaat cgcaataa
//
LOCUS       DALAGF010000200         1545 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.209, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000200 DALAGF010000000
VERSION     DALAGF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.209"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            9..>1545
                     /locus_tag="O2692_002756"
     CDS             9..>1545
                     /locus_tag="O2692_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HCZ4300335.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAILAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWK"
BASE COUNT          455 a          238 c          311 g          541 t
ORIGIN      
        1 tgactgttat gtcagaagaa aaacatgtag ttgaacatga acaacaaaag aaagaaaaga
       61 caaaaaagca atacaagcca ttttggattg tcatgagttt tataatactt atagttgtac
      121 tattactccc ggcaccttca agtctgccga taatggctaa ggcagtacta gctattttag
      181 cttttgcagt tattatgtgg gtaacggaag ctgtatcata tccggtgtca gcaactttaa
      241 ttattggctt aatgatatta cttttaggat ttagccctgt tcaaaattta ggggagaagc
      301 taggtaatcc gaaaagtggc agtgctattt tagctggaag tgaccttcta ggaactaatc
      361 atgcattatc attagcgttt agtggatttg caacttcagc tgtagctctc gttgcagctg
      421 cattattttt ggctgctgct atgcaagaaa cgaatttgca taaaagacta gctcttttag
      481 tgttatcaat tgttggtaat aaaactagaa atatagttat tggagcaatt atcgtttcaa
      541 ttgtacttgc atttttcgtt ccttctgcaa cagctagagc aggggcagtt gtaccaatct
      601 tgctgggtat gattgcggca tttaaagttt ccaaagatag caagttagcg tctttattaa
      661 taattacttc agtacaagct gtgtcaattt ggaatattgg tatcaaaacg gcggcagcac
      721 aaaatatcgt agcgattaat tttataaacc atcaattagg atttgatgtt tcatggggcg
      781 agtggttctt atatgcagcg ccttggtcca tagttatgtc cgtagcttta tatttcatca
      841 tgattaaagt gatgcctcca gaaattaata caatagaagg tggtaaagat ttaataaaag
      901 aagaattgca taaacttggc cccgttagcc cacgtgaatg gcgtttaatt gttatatcga
      961 tgttattatt actgttttgg tcaactgaaa aagtattaca tccgattgac tctgcatcca
     1021 ttactattat tgctttaggt gttatgttaa tgccgaaaat tggtgtcatg acatggaaac
     1081 atgttgaaaa taaaatacca tggggaacaa ttatcgtgtt tggtgtaggt atttcactag
     1141 gtaacgttct tttgaaaaca ggtgcagctc aatggttaag tgatcaaact tttggtgttt
     1201 taggtttaaa acatttacct attatcgcga caattgcgct tatcacgctt tttaatatat
     1261 tgattcattt gggctttgcg agtgcaacaa gtttatcatc agcgttaata cctgttttta
     1321 tttcgctaac ctctacgtta cacttaggag accagtctat aggatttgtt ttaattcaac
     1381 aatttgttat tagttttggt ttcttattac ctgttagtgc acctcaaaat atgttggctt
     1441 atggcactgg tacttttacg gttaaagatt tcttgaaggc aggtatacca ttgacaattg
     1501 tagggtatat tctagtgata gtttttagca tgacttattg gaaat
//
LOCUS       DALAGF010000201         1490 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.210, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000201 DALAGF010000000
VERSION     DALAGF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.210"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            270..1229
                     /gene="hyl"
                     /locus_tag="O2692_002757"
     CDS             270..1229
                     /gene="hyl"
                     /locus_tag="O2692_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HCZ4300336.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWIDRSSERYKIDWEKEEMTN"
BASE COUNT          564 a          219 c          230 g          477 t
ORIGIN      
        1 agctatgtct tttccttgtt tcataaagaa tttattcaac tttgactaac cctcgaaatt
       61 gaaatgcttc ctttcaaatt ttaaataaaa gttaaaaaca tattcttaaa ataatcactc
      121 atcatcactc agtaatttat cagttgctac attaaatata ctaatataaa cgattataaa
      181 tatttgatat gtctcaactg caatattcta aattgacata ttgattattg tttcctcaaa
      241 tcgttttaaa aaatagaagg atgatgaaaa tgaaaacacg tatagtcagc tcagtaacaa
      301 caacactatt gctaggttcc atattaatga atcctgtcgc taatgccgca gattctgata
      361 ttaatattaa aaccggtact acagatattg gaagcaatac tacagtaaaa acaggtgatt
      421 tagtcactta tgataaagaa aatggcatgc acaaaaaagt attttatagt tttatcgatg
      481 ataaaaatca taataaaaaa ctgctagtta ttagaacgaa aggtaccatt gctggtcaat
      541 atagagttta tagcgaagaa ggtgctaaca aaagtggttt agcctggcct tcagccttta
      601 aggtacagtt gcaactacct gataatgaag tagctcaaat atctgattac tatccaagaa
      661 attcgattga tacaaaagag tatatgagta ctttaactta tggattcaac ggtaatgtta
      721 ctggtgatga tacaggaaaa attggcggcc ttattggtgc aaatgtttcg attggtcata
      781 cactgaaata tgttcaacct gatttcaaaa caattttaga gagcccaact gataaaaaag
      841 taggctggaa agtgatattt aacaatatgg tgaatcaaaa ttggggacca tatgatagag
      901 attcttggaa cccggtatat ggcaatcaac ttttcatgaa aactagaaat ggctctatga
      961 aagcagcaga taacttcctt gatcctaaca aagcaagttc tctattatct tcagggtttt
     1021 caccagactt cgctacagtt attactatgg atagaaaagc atccaaacaa caaacaaata
     1081 tagatgtaat atacgaacga gttcgtgatg actaccaatt gcactggact tcaacaaatt
     1141 ggaaaggtac caatactaaa gataaatgga tagatcgttc ttcagaaaga tataaaatcg
     1201 attgggaaaa agaagaaatg acaaattaat gtaaattatt tgttcatgta caaataaata
     1261 taatttataa ctttagccga taacttcaga aatgatgttt ttcggctaat ttttatactg
     1321 aatttagttt aattaactta tgatattttt aatttaaact atctacttga tttgctttcc
     1381 tgacttaaaa attagactaa acatgacact atatgaggta atttttaaaa taagaaattt
     1441 gaattccatg ttgtcaaacc ctttatataa aagttttgaa attgttcaaa
//
LOCUS       DALAGF010000202         1472 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.211, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000202 DALAGF010000000
VERSION     DALAGF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.211"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..220
                     /locus_tag="O2692_002758"
     CDS             <1..220
                     /locus_tag="O2692_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ4300337.1"
                     /translation="TLKSLCLSIVLYIYLGGGSFFTIYMLLTIEVQNIILFITLFVIF
                     LFFLFLNMCSLYDNKVHVLFKSNGIEKF"
     gene            257..892
                     /locus_tag="O2692_002759"
     CDS             257..892
                     /locus_tag="O2692_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCZ4300338.1"
                     /translation="MLCESKVINKNPKYRVIKYGDEYLMIDLVSTWLTLFLPMINWLI
                     PKKYVKISKKEFDDLNIVKPVKNKAFWPVAGSTILFGVTFRKYIPSLNIQLEKNMVIV
                     ICCAIFLGVLILFLFLNRKLRLEIYNNNSSKGKIILFPSLKNFCFTIFYYFLFGGLSI
                     MALSMLLTLNPQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVLSKTNTVEK"
     gene            858..1103
                     /locus_tag="O2692_002760"
     CDS             858..1103
                     /locus_tag="O2692_002760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300339.1"
                     /translation="MYYLKLTRWKNDGLAGFTAGSISAILVYWTNQKNEFGIKDKNDW
                     IGHKLDVGIDAVEKSAEKTVDGVENVMVKLQKVFLII"
     gene            1127..>1472
                     /locus_tag="O2692_002761"
     CDS             1127..>1472
                     /locus_tag="O2692_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ4300340.1"
                     /translation="MLLCESKIINKNPKYRIIKYNDEYLMVDIISTWISLFFPFINWF
                     IPKEYVKISREEFENLNIVKPAKKNVFWPVAGSSALLGVALRKYTHLLDIQLDKKLVI
                     AICCITFIGILIF"
BASE COUNT          525 a          158 c          234 g          555 t
ORIGIN      
        1 cactttaaag agtctttgtt tatcaatagt tttatatatt tatttaggtg gtggttcatt
       61 ctttactatt tatatgttat tgacgattga agttcaaaat ataatattat ttataacttt
      121 gtttgtaata tttttgtttt tcttattttt aaatatgtgc tcactatatg acaataaagt
      181 tcatgtatta tttaaatcaa atggaattga aaagttttaa taggaataat aaacaaagga
      241 atggtagtga taaatattgc tatgtgaatc taaagttatc aacaaaaatc ctaagtatag
      301 agttattaaa tatggtgatg aatatttaat gattgattta gtaagtacct ggttaacttt
      361 atttcttcct atgattaatt ggttaattcc aaaaaaatat gtcaaaatca gtaaaaaaga
      421 atttgacgat ttaaacattg tcaaacctgt taaaaataaa gctttttggc cagttgcagg
      481 tagtactatt ttgttcggag ttacgtttag aaaatatatt ccttcactta atattcaatt
      541 agagaaaaac atggtgattg taatatgttg tgcaatattt ctgggtgttt taatactttt
      601 tttatttctg aatcgtaagc taaggttgga aatttataat aataactcta gtaaagggaa
      661 aataatttta tttccttcat taaaaaactt ttgtttcaca atattttatt attttttatt
      721 tggcggtctt tcaataatgg ctctaagtat gttattaact ttaaatcctc aaaatataat
      781 aggctttatt ggttggttgg taatgactgc aggtttcttt ctgttaaaca tgtcatcgat
      841 tattgacaaa aaaatttatg tattatctaa aactaacacg gtggaaaaat gatggtttag
      901 ctggatttac tgcaggttct atttcggcaa tacttgtata ttggaccaat caaaaaaatg
      961 aatttggaat aaaagataaa aacgattgga taggacataa actagacgtt ggtatagatg
     1021 ctgtagaaaa atctgcagaa aaaacagtag atggtgttga aaatgtcatg gtgaagcttc
     1081 aaaaagtatt tctaatcata taagccctaa gaaatggagc tggtaaatgt tgctatgcga
     1141 atctaaaatc atcaataaaa acccaaaata tagaattatt aaatataatg atgaatactt
     1201 aatggtcgat ataataagca cttggattag tttatttttt ccttttatta attggttcat
     1261 cccaaaagaa tacgtcaaaa ttagtagaga agagtttgaa aacttaaata ttgttaaacc
     1321 tgctaaaaag aatgtttttt ggccagttgc aggtagctct gctttgctgg gagttgcatt
     1381 aagaaaatat acacatttac ttgacattca acttgataaa aaattagtta ttgccatatg
     1441 ttgcatcaca tttataggga ttttaatatt tt
//
LOCUS       DALAGF010000203         1466 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.212, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000203 DALAGF010000000
VERSION     DALAGF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.212"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(244..732)
                     /locus_tag="O2692_002762"
     CDS             complement(244..732)
                     /locus_tag="O2692_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HCZ4300341.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEVVF
                     NYTKPGSEDLNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKKEDLEPWTSCEF
                     DFTRKGNLKVSFDYIDWIKLGFGPSGKENYYMYKKFGILPDMEYEMEEIRAVEKYVKE
                     QE"
     gene            complement(743..1243)
                     /locus_tag="O2692_002763"
     CDS             complement(743..1243)
                     /locus_tag="O2692_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ4300342.1"
                     /translation="MTFEEKISKLYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPGSDDLNYYTDIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEGLEPWTSCEF
                     DFTSEGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEMEEIKEIDQYIKE
                     QDEAEI"
     gene            complement(1254..>1466)
                     /locus_tag="O2692_002764"
     CDS             complement(1254..>1466)
                     /locus_tag="O2692_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ4300343.1"
                     /translation="WTSCEFDFTRDGKLNVSFDYIDWINTEFDQLGRQNYYMYKKFGV
                     IPEMEYEMEEVKEIEQYIKEQEEAEQ"
BASE COUNT          448 a          294 c          138 g          586 t
ORIGIN      
        1 agttttaaaa aataagtcga tgtacatcaa ctttataaaa tacactacaa taaccttttc
       61 aattaatgac aattttaaat ttatatcgtg atttgttaat tagttgaact catttatcca
      121 tgatgtatgg ataccgataa gctaattgac tttttaagtt gcaaataatc ttatctttta
      181 ccttcttctt tgaatcaaat gtaaaacgag ggtgattttg cacagtcttt tataacatgt
      241 ctgctactct tgctctttaa catacttctc tactgctcga atttcttcca tttcatattc
      301 catatctggt aaaataccaa attttttgta catatagtag ttttcctttc ctgatgggcc
      361 aaaacctaat ttaatccaat ctatataatc aaatgatact tttaaatttc cctttcttgt
      421 aaagtcaaat tcacatgatg tccacggttc taaatcttct tttttaaaag tttctcttaa
      481 ctcatcaaac attcgataaa ctttaaacca tgaattctta aaaatatctt ttgagacatt
      541 gcaatcttta ggaatatctg aataataatt caaatcttca cttcctggtt tagtataatt
      601 aaaaacaacc tctccacctt catcatctac gtaggcaatt gtatatactt tttcccattc
      661 tactggtatc attccactga tttcatttgc aatttcattg tacatttgac ttagcttttc
      721 ttcgaaagtc atgttatctc ccctatattt cagcttcatc ttgctcttta atatattgat
      781 cgatttcttt aatttcttcc atttcatatt ccatttctgg taaaacccca aattttttat
      841 acatataata attttcacgg cctaattgat caaactctgt atttatccaa tctatataat
      901 caaatgaaac ttttaattta ccttcgcttg taaagtcaaa ttcacatgat gtccatggtt
      961 caagtccttc ttctttaaat aaatctctta attcctcaaa caaatcatat aaatccatcc
     1021 ataaatcatc aaatacttgc acagagatgt tatactcctt aggtatatcg gtgtaataat
     1081 tcaagtcatc actacctggt ttagtataat taaagaatac ttcacctcct ccatcatcta
     1141 tataagccat tgtatatact ttttcccact ctaccggtat catactgcta atctcattcg
     1201 caatctcatt atataatttg cttattttct cttcgaaagt catgttatca cctctattgt
     1261 tcagcttctt cttgctcttt aatatattgt tcgatttctt taacttcttc catttcatat
     1321 tccatttctg gtataacccc aaattttttg tacatataat aattttgacg gcccaattga
     1381 tcaaactctg tatttatcca atctatataa tcaaaagata cattcaattt gccatctctt
     1441 gtaaagtcaa attcgcatga tgtcca
//
LOCUS       DALAGF010000204         1432 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.213, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000204 DALAGF010000000
VERSION     DALAGF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.213"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            21..>1432
                     /locus_tag="O2692_002765"
     rRNA            21..>1432
                     /locus_tag="O2692_002765"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          428 a          270 c          413 g          321 t
ORIGIN      
        1 ataaaaatgg tggaaacata gattaagtta ttaagggcgc acggtggatg ccttggcact
       61 agaagccgat gaaggacgtt actaacgacg atatgctttg gggagctgta agtaagcttt
      121 gatccagaga tttccgaatg gggaaaccca gcatgagtta tgtcatgtta tcgatatgtg
      181 aatacatagc atatcagaag gcacacccgg agaactgaaa catcttagta cccggaggaa
      241 gaaaaagaaa attcgattcc cttagtagcg gcgagcgaaa cgggaagagc ccaaaccaac
      301 aagcttgctt gttggggttg taggacactc tatacggagt tacaaaggac gacattagac
      361 gaatcatctg gaaagatgaa tcaaagaagg taataatcct gtagtcgaaa atgttgtctc
      421 tcttgagtgg atcctgagta cgacggaaca cgtgaaattc cgtcggaatc tgggaggacc
      481 atctcctaag gctaaatact ctctagtgac cgatagtgaa ccagtaccgt gagggaaagg
      541 tgaaaagcac cccggaaggg gagtgaaata gaacctgaaa ccgtgtgctt acaagtagtc
      601 agagcccgtt aatgggtgat ggcgtgcctt ttgtagaatg aaccggcgag ttacgatttg
      661 atgcaaggtt aagcagtaaa tgtggagccg tagcgaaagc gagtctgaat agggcgttta
      721 gtatttggtc gtagacccga aaccaggtga tctacccttg gtcaggttga agttcaggta
      781 acactgaatg gaggaccgaa ccgacttacg ttgaaaagtg agcggatgaa ctgagggtag
      841 cggagaaatt ccaatcgaac ctggagatag ctggttctct ccgaaatagc tttagggcta
      901 gcctcaagtg atgattattg gaggtagagc actgtttgga cgaggggccc ctctcgggtt
      961 accgaattca gacaaactcc gaatgccaat taatttaact tgggagtcag aacatgggtg
     1021 ataaggtccg tgttcgaaag ggaaacagcc cagaccacca gctaaggtcc caaaatatat
     1081 gttaagtgga aaaggatgtg gcgttgccca gacaactagg atgttggctt agaagcagcc
     1141 atcatttaaa gagtgcgtaa tagctcacta gtcgagtgac actgcgccga aaatgtaccg
     1201 gggctaaaca tattaccgaa gctgtggatt gtcctttgga caatggtagg agagcgttct
     1261 aagggcgttg aagcatgatc gtaaggacat gtggagcgct tagaagtgag aatgccggtg
     1321 tgagtagcga aagacgggtg agaatcccgt ccaccgattg actaaggttt ccagaggaag
     1381 gctcgtccgc tctgggttag tcgggtccta agctgaggcc gacaggcgta gg
//
LOCUS       DALAGF010000205         1355 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.214, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000205 DALAGF010000000
VERSION     DALAGF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.214"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..1318
                     /locus_tag="O2692_002766"
     rRNA            <1..1318
                     /locus_tag="O2692_002766"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          302 c          399 g          296 t
ORIGIN      
        1 cactgccgag aaaagcctct agatagaaaa taggtgcccg taccgcaaac cgacacaggt
       61 agtcaagatg agaattctaa ggtgagcgag cgaactctcg ttaaggaact cggcaaaatg
      121 accccgtaac ttcgggagaa ggggtgctct ttagggttaa cgcccagaag agccgcagtg
      181 aataggccca agcgactgtt tatcaaaaac acaggtctct gctaaaccgt aaggtgatgt
      241 ataggggctg acgcctgccc ggtgctggaa ggttaagagg agtggttagc ttctgcgaag
      301 ctacgaatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa
      421 cgagagactc ggtgaaatca tagtacctgt gaagatgcag gttacccgcg acaggacgga
      481 aagaccccgt ggagctttac tgtagcctga tattgaaatt cggcacagct tgtacaggat
      541 aggtaggagc ctttgaaacg tgagcgctag cttacgtgga ggcgctggtg ggatactacc
      601 ctagctgtgt tggctttcta acccgcacca cttatcgtgg tgggagacag tgtcaggcgg
      661 gcagtttgac tggggcggtc gcctcctaaa aggtaacgga ggcgctcaaa ggttccctca
      721 gaatggttgg aaatcattca tagagtgtaa aggcataagg gagcttgact gcgagaccta
      781 caagtcgagc agggtcgaaa gacggactta gtgatccggt ggttccgcat ggaagggcca
      841 tcgctcaacg gataaaagct accccgggga taacaggctt atctccccca agagttcaca
      901 tcgacgggga ggtttggcac ctcgatgtcg gctcatcgca tcctggggct gtagtcggtc
      961 ccaagggttg ggctgttcgc ccattaaagc ggtacgcgag ctgggttcag aacgtcgtga
     1021 gacagttcgg tccctatccg tcgtgggcgt aggaaatttg agaggagctg tccttagtac
     1081 gagaggaccg ggatggacat acctctggtg taccagttgt cgtgccaacg gcatagctgg
     1141 gtagctatgt gtggacggga taagtgctga aagcatctaa gcatgaagcc cccctcaaga
     1201 tgagatttcc caacttcggt tataagatcc ctcaaagatg atgaggttaa taggttcgag
     1261 gtggaagcat ggtgacatgt ggagctgacg aatactaatc gatcgaagac ttaatcaaaa
     1321 taaatgtttt gcgaagcaaa atcactttta cttac
//
LOCUS       DALAGF010000206         1262 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.215, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000206 DALAGF010000000
VERSION     DALAGF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.215"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..351
                     /gene="lpl8"
                     /locus_tag="O2692_002767"
     CDS             <1..351
                     /gene="lpl8"
                     /locus_tag="O2692_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HCZ4300344.1"
                     /translation="NLKKRIENFKFFGQYANLKELKNYNNGDVSINENVPSYDAKFKM
                     SNKDENVKQLRSRYNIPTDKAPVLKMHIDGNLKGSSVGYKKLEIDFSKGGKSDLSVID
                     SLNFQPAKVDEDDE"
     gene            370..1155
                     /gene="lpl9"
                     /locus_tag="O2692_002768"
     CDS             370..1155
                     /gene="lpl9"
                     /locus_tag="O2692_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl9"
                     /protein_id="HCZ4300345.1"
                     /translation="MKSIKRIGLCISLLILIIFVTSCDGDNKIIGDSKEEQIKKSFAK
                     TLDIYPIKNLEDLYDKEGYRDGEFKKDDKGTWLIRSEMKIQLKGENLESRGAVLEINR
                     NTRTAKGHYIVREVVEDSDGMTHNHTKRYPVKMENNKMIPLKPIDDEKVKKEIEEFNF
                     FVQYGNFKELENYKEDEVSYNPEVPIYSAKYQLKNSDYNVEQLRKRYNIPTQKAPKLL
                     LKGSGNLKGSSVGYKNIEFTFIENKEENIYFTDSIYFNPSEDK"
BASE COUNT          537 a          139 c          231 g          355 t
ORIGIN      
        1 aatctaaaaa agagaataga aaactttaaa ttttttggac aatatgcaaa ccttaaagaa
       61 ttgaaaaact acaacaatgg tgatgtctca attaatgaga atgttccaag ttatgacgca
      121 aaatttaaaa tgagcaataa agatgaaaat gttaagcaat taagaagtcg ttataatatt
      181 cctactgata aagcaccggt attaaaaatg catattgatg gtaatttgaa aggaagttct
      241 gtgggttata aaaagttgga aattgacttt tcaaaaggtg gaaaaagcga tttgtcagta
      301 atagattctt tgaatttcca gccggcgaag gtagatgaag atgatgaatg atgaggatgg
      361 tgtgtaacaa tgaagtctat aaaaaggatt ggattgtgca ttagtttgtt gattttaatc
      421 atctttgtta catcttgtga tggtgataat aagatcattg gagattcaaa agaagaacaa
      481 atcaaaaaga gctttgcgaa aacgttagat atctacccta ttaagaatct cgaggattta
      541 tacgacaaag aaggatatcg agatggcgaa tttaaaaaag atgataaagg tacttggcta
      601 attagatctg aaatgaaaat ccaattaaaa ggagaaaatc tggaatctag aggagcagtt
      661 ttagaaatta acagaaatac tagaacggct aaagggcatt atattgttag agaagttgtt
      721 gaagatagcg atggaatgac acacaatcat acaaaaagat atcctgtgaa aatggaaaat
      781 aataaaatga ttccattaaa accaatcgat gacgaaaaag taaaaaaaga aatcgaagaa
      841 tttaacttct ttgtacaata cgggaatttc aaagaattgg aaaactataa agaggacgaa
      901 gtgtcatata acccagaagt accaatttac tctgcaaaat atcaattgaa aaacagtgat
      961 tacaatgttg aacaattaag gaagcgatat aatatcccga cgcaaaaagc gcccaaatta
     1021 ttattgaaag gctcaggtaa tttaaaaggt tcatcagtcg gatataaaaa tattgaattt
     1081 acctttattg aaaacaagga agaaaatatt tacttcacag atagtatcta ctttaatcca
     1141 agcgaggata aataaatata attactaata taaaaaataa cgttagaaca tttaataata
     1201 aagttaaaat gaattacatg tattaaattg aataagttag ttttgtttga taacataaaa
     1261 gt
//
LOCUS       DALAGF010000207         1235 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.216, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000207 DALAGF010000000
VERSION     DALAGF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.216"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            58..852
                     /locus_tag="O2692_002769"
     CDS             58..852
                     /locus_tag="O2692_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCZ4300346.1"
                     /translation="MIHSKKLTLGICLVLLIILIVGYVIMTKTNGRNAQIKDTFNQTL
                     KLYPTKNLDDFYDKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNT
                     RTTKGYYFISEMTDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLKKEIENFKFFV
                     QYGNFKDINDYKDGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDIPTKQAPKLLLK
                     GDGDLKGSSVGSRSLEFTFVENKEENIYFTDSVQYTPSEDTRYESN"
     gene            complement(856..978)
                     /locus_tag="O2692_002770"
     CDS             complement(856..978)
                     /locus_tag="O2692_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300347.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
BASE COUNT          504 a          162 c          217 g          352 t
ORIGIN      
        1 ctttagttgt aaatattaaa aactgagtca atattaaaaa tataaggtgg agaaatgatg
       61 attcattcaa agaaattaac gctcggtata tgcttggtat tactcattat attgattgta
      121 ggttatgtca ttatgacaaa aacaaatggt cgaaacgccc aaattaaaga cacatttaat
      181 caaacattaa aattatatcc aaccaaaaat ctcgatgatt tttacgataa agaaggcttt
      241 cgagatcaag aatttaaaaa gggtgataaa ggtacttgga tagttaattc tgaaatggta
      301 atcgagccaa aaggtaagga tatggaaacg agaggaatgg tgctctatat caatcgcaat
      361 actagaacca caaaagggta ttattttata agtgaaatga cagatgacag taacggcaga
      421 ccaaaggatg atgaaaaaag gtatccggta aaaatggaac ataataaaat cataccaacg
      481 aagccactac cgaatgacaa gttaaaaaaa gagattgaaa actttaagtt ctttgtacaa
      541 tatggcaact ttaaagatat taatgattat aaagatggtg atatttcata taatcctaat
      601 gtaccaagtt attcggcaaa atatcaattg aataatgatg attataatgt ccaacagtta
      661 agaaaaagat atgatattcc aaccaaacaa gcgccgaaac tattattgaa aggcgatgga
      721 gatttaaaag gttcatccgt aggttctaga agtcttgaat ttacctttgt cgaaaataaa
      781 gaagaaaaca tttattttac agattctgta caatatactc caagtgagga tacaaggtat
      841 gagtcaaact gaatatcaaa taaaacctgg taatataaca agtaactctg aagaaacaag
      901 ttcgatatct aaagtgagct gtgaaatata ggtagccatt tcaaaaaatt taaaggtgaa
      961 tttgataatg tagctcaagg agattgggtt aaaaaggcga agaatgaact ggatgatatt
     1021 agtaagaaat taaaaaatat tcaaagaacg gaagtttaat agcttatatg attcttggag
     1081 ctaagacagc atgcgttcat tcatgccatt attaatataa gcaccgcaac aaaaaagctt
     1141 ctaatgtgat acaggaacct catattccgt atcatgttag aagcttttaa tgtctaaaga
     1201 acatctacat tttatcatat tttctgactt attaa
//
LOCUS       DALAGF010000208         1216 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.217, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000208 DALAGF010000000
VERSION     DALAGF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.217"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            141..>1216
                     /locus_tag="O2692_002771"
     CDS             141..>1216
                     /locus_tag="O2692_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCZ4300348.1"
                     /translation="MGYRTLKSIFHEHNESKMKEEYIKRFNSLASFNTNINIIPMENG
                     KKVNDLEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQS
                     TNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDK
                     LVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEFIGEMLTFLKYFD
                     APQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLSDYYDNYTALTFSYVINRNKSK
                     YYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYK
                     QIDYEFLYLLSMDKLFGNKRNRLT"
BASE COUNT          482 a          128 c          199 g          407 t
ORIGIN      
        1 tgaaaaatct ttttctattg attgagaaaa tagttattta tatagttttt aaagaaaaaa
       61 agtgataata tttagctaat ctaatgaaaa ttgatttatt ggacaaaaaa tacattttaa
      121 atgagtagag gaggctgcaa atgggttaca gaactttaaa aagtattttt catgaacaca
      181 atgaaagtaa aatgaaggaa gagtatatta aaagatttaa ttctttagct tctttcaata
      241 ctaatattaa tatcatacct atggaaaatg gaaaaaaagt taatgatttg gaatatcctc
      301 tattctttat ggtgactaaa aatctatcaa aaaaacaaga attaatatca attaatagta
      361 gaaaaattga tagagcactt aattctttac catatgcagc tagagaacaa tattttaatg
      421 atttattaat cgatgaatta caaagtacta atgaaattga aaatgtattt agtactaaac
      481 aagagattgc acatgcgtta aataaccaag catcagaatt tcttaagttc agaggcctcg
      541 tggatcaata taaagagata gaacttaata aaaaaattaa agttgataat gtaagagaca
      601 ttagagcgat ttatgataaa ttagtttcaa atgaaattaa cgaacaagat aagttagatg
      661 gagagctatt tcgtaaaaat tttgtcggtg tgcatgatgg gtcaacgaat aaatatatac
      721 atgttgggtt acaacctgaa accaaaattg ttgaatttat aggtgaaatg ctaacatttt
      781 taaaatattt tgatgcgcct cagccgttca aaatcatggc tagtcattat ctgtttgaat
      841 atatacatcc tttttatgat ggcaatggta gagttggaag atttataatt gccaagctct
      901 tgagtgatta ttatgataat tatactgctt tgacattttc atatgttata aatagaaata
      961 aatcgaaata ttacaaggcg tttatgactg cttcaaatca tttgaattgt ggtgatttga
     1021 cggaatttat tgacacaatg cttgagctat tgatagcagg acaagaaaga atacttgatg
     1081 agttaatacc aaaaatggat gcgactgaaa aattaacgct ttatttaact agtcattata
     1141 aacagataga ttatgaattt ttgtatctcc tttcaatgga taaattgttt ggaaataaaa
     1201 gaaatagatt aacatt
//
LOCUS       DALAGF010000209         1185 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.218, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000209 DALAGF010000000
VERSION     DALAGF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.218"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            103..451
                     /locus_tag="O2692_002772"
                     /pseudo
     CDS             103..451
                     /locus_tag="O2692_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456552.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            462..656
                     /locus_tag="O2692_002773"
     CDS             462..656
                     /locus_tag="O2692_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300349.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
     gene            697..1047
                     /locus_tag="O2692_002774"
     CDS             697..1047
                     /locus_tag="O2692_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300350.1"
                     /translation="MLEMISNGKMTMKLNNVKQKRHILCTNEYNNKKNNSSLLPSYTI
                     IDSNESEKMTKKEFIDIPVLFDDEGNFRIKQVIDYKKIIGKSYVNGKYIETKLGKVHY
                     SKTGFHVVPYIKKE"
     gene            1054..>1185
                     /locus_tag="O2692_002775"
     CDS             1054..>1185
                     /locus_tag="O2692_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HCZ4300351.1"
                     /translation="MNLQSYIGKLVKLTLTNNKILIGKVIDFDDKVDNFDGYNSIEID
                     "
BASE COUNT          515 a          100 c          207 g          363 t
ORIGIN      
        1 ggcaattggc gggacaagtt ctttaaagag cgtgaaggta aatatcaagt agaagtaaaa
       61 gaagcataat tacaggaaaa agctaaaaac cagatgaaag aaatgattaa aaatggtaaa
      121 ataaaaatag aaataaattg cggaaaaata aaatagatat atgttaggtc atcacctata
      181 taatgaaaat aaaaaaagag ccattttaaa taataagaaa ttgcctagct atacaatact
      241 ttctatagat ttattgaatg aattgttaag agaaaaaatg tcaacaggca atctaatatt
      301 aagtgatgag cgatttgatt tgaaagagat tattaatttt aatcaaatta ttggaaaaat
      361 acatatcgaa aatgtgtata ttgaaaccag aaagggaaaa gtgcattatt cgaagacagg
      421 tgctcatata gtaccttata ttgataagta ggtgaaaagt attgagaatt gaagatgcat
      481 atcgtaaaga tgttattatc acgcttttga ataatgaaga atacgaaggg tttgtaactg
      541 actatgaaaa tgaattcgag agcgaaacag gaaatcttgt tgtagatata cagactgatt
      601 ttgctgttta ttcgtttgat gaaactgaga taaaaagtat tagattatta aaataattac
      661 aaaatagcat cctttctaca aaaagctaaa aaagaaatgc ttgaaatgat aagtaatggt
      721 aaaatgacga tgaaattgaa taatgttaag caaaagcgtc atattttatg cacaaatgaa
      781 tataataata agaaaaataa ttcatcttta ttaccaagtt atactataat agattctaat
      841 gaaagtgaaa agatgactaa aaaagaattt attgatattc cagttttgtt tgatgatgaa
      901 gggaatttta gaataaaaca agttatagat tataaaaaga taataggtaa atcttatgtg
      961 aatggtaaat atattgaaac gaaattaggt aaggttcatt attctaaaac tggttttcat
     1021 gtagttccgt atattaaaaa ggagtgatta aatatgaatt tacagtcata cataggcaaa
     1081 ttagtcaaat taacgcttac taataataaa atattaattg gaaaagtgat agactttgat
     1141 gataaagttg ataattttga tggttataat tcgatagaaa ttgat
//
LOCUS       DALAGF010000210         1159 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.219, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000210 DALAGF010000000
VERSION     DALAGF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.219"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            116..259
                     /locus_tag="O2692_002776"
     CDS             116..259
                     /locus_tag="O2692_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HCZ4300352.1"
                     /translation="MEKVLDLDVQVKANNNSNDSAGDERITSHSLCTPGCAKTGSFNS
                     FCC"
     gene            324..>1159
                     /locus_tag="O2692_002777"
     CDS             324..>1159
                     /locus_tag="O2692_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HCZ4300353.1"
                     /translation="MNTIYEPSSICMIRTPLLSVEFFNLFLNTEQIKYSDLQLNAQMK
                     ESILTTTFNLYCTLQEINFDGDNKKVRDAKESLLKYLIRMSTRPTPFGLLSGINLGHF
                     VNEPTRLKVGNSIQKYVKVDGEWLYKLVSYIESIDEYYQNLKVIWNSKAHIINDRIYL
                     NEQSAIYLNNNKDTSFSIKNSELLVFIKTTVTNNNITFSNLAEKINQEFEINDITKVK
                     VYIHNLVSKEIIYSTIRPPLSYSDNLNYILNKLSLHNDDFVKKIREIQKLILAYEKTE
                     IG"
BASE COUNT          455 a          137 c          175 g          392 t
ORIGIN      
        1 tttacaaaaa attcacaaaa tataattata taatttattg atttgttgtg tgaaaaggga
       61 taatatgaca attgaagtag gccttaactt caactaataa taggaggttt tatctatgga
      121 aaaagttctt gatttagacg tgcaagttaa agcaaacaat aactcaaatg attcagcagg
      181 tgacgaacgt attacaagtc atagtttatg tactcctggt tgtgctaaga ctggtagttt
      241 taatagcttc tgctgttaag attacagcaa atagagcctg aaagggctct atttgtctat
      301 tcaaatatgt gaggtgctta caaatgaaca ctatttacga accgtcaagt atttgtatga
      361 ttagaacacc tttattatca gttgaatttt tcaatttatt tttaaatact gaacaaatta
      421 aatatagcga cttacaatta aatgctcaga tgaaagaatc aatcctaaca acaacattta
      481 acttatattg tactttacaa gaaataaatt ttgatggcga taacaagaag gttagagatg
      541 ctaaagaaag tttattaaaa tatttaatta gaatgtcaac acgtccaact ccatttggat
      601 tattaagtgg tattaatttg gggcattttg taaatgaacc aacacgtctt aaagttggaa
      661 atagtattca aaaatatgtg aaagtcgatg gcgaatggtt gtataaactg gttagttata
      721 ttgagagtat tgatgaatat tatcaaaatt tgaaagttat ttggaatagt aaagcacata
      781 ttatcaatga tcgaatatat ttaaatgaac aaagtgctat ttatttaaac aacaataaag
      841 atacatcttt ttccattaaa aatagcgaat tactcgtatt catcaaaacc actgtgacga
      901 ataataatat tactttttca aatttagctg aaaagataaa tcaagaattt gaaattaatg
      961 atattactaa agtgaaagta tatatccata atcttgtctc aaaggaaatt atatattcaa
     1021 caatcagacc accgttatca tatagcgata atttaaatta tattttaaat aaactttctt
     1081 tacataatga tgattttgtg aaaaaaatta gagaaatcca gaaattgata ttagcttatg
     1141 aaaaaactga aattggatt
//
LOCUS       DALAGF010000211          996 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.220, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000211 DALAGF010000000
VERSION     DALAGF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.220"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(162..854)
                     /locus_tag="O2692_002778"
     CDS             complement(162..854)
                     /locus_tag="O2692_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCZ4300354.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFNFADVTKEKCIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKI"
BASE COUNT          305 a          196 c          139 g          356 t
ORIGIN      
        1 cctagtgtaa tatatgtgta attctaaaag attcttcttt aaaaatataa ataccacgac
       61 atattgcttt aacattttca tttataaagc gaaaaaatgc atcgctacta agttgaatgt
      121 ttagtaagga tgcatttaat tcactaaaat gattaaatta cttatatctt ttcatctgat
      181 tgattatcga aatttcttcc ttctaaacct gctaactctt ctttagaagc tgcaggtgct
      241 ttcatttcaa atatctcatt cactactgtg taatcgtaat atcctaatct ggcaataggt
      301 ttaatcgact taatgtccaa tttaccatta tcaagaataa ccttatcgtc aatatgaact
      361 tgggcaactc ttcctataac aatatctacg gtagatactg gatctccagt tggaatacga
      421 atcgtttgaa cgtactcaca ttcaaaatga actggcgatt cttttacacg atatcctgga
      481 gcttctatac atttttcctt tgttacatct gcaaaattaa attcatcctc ttctggtggc
      541 aatgctttcg atgataaatt aactgcttct cttaaatcat acgttgccat attccacaca
      601 aaccaacctg tctcttcagc atttttcact gtatctttac gttcgtgatc accaagaacg
      661 gattgatttg ctgcgaacat aaccataggc ggatcccaag ttaagttttg atactgacta
      721 taaggcgcta aattatcttt cccatctttc gatacagtag agatccaccc tattggacgt
      781 ggtactgtac tacttttaaa tgggtcgtgc ggtaaaccat gacttcttac accttgtttt
      841 ggcgaataat tcatactatc ttcacccctt ataagtaatt acatttaagg ttacgccctc
      901 tttacataag cgtctaatat aaataaacaa tttatttata agtagaaact atatatgacg
      961 tggttgctta taatttgcgt tcttgattcg aaaaat
//
LOCUS       DALAGF010000212          972 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.221, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000212 DALAGF010000000
VERSION     DALAGF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.221"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(201..305)
                     /locus_tag="O2692_002779"
     CDS             complement(201..305)
                     /locus_tag="O2692_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300355.1"
                     /translation="MLNIKAIKPSEKGIKVLSHQFKQVSKTQLNYFFN"
     gene            complement(468..602)
                     /locus_tag="O2692_002780"
     CDS             complement(468..602)
                     /locus_tag="O2692_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300356.1"
                     /translation="MTDKNGHGIAITGIILFVINALQINNDLPISISSKLKKYIHKPD
                     "
     gene            complement(696..953)
                     /locus_tag="O2692_002781"
     CDS             complement(696..953)
                     /locus_tag="O2692_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300357.1"
                     /translation="MEIIIEIYMYLRDGIKEHFILKKVRKKFKKLNEINSDIQNMYAE
                     NQGLLETDKTIIDKVLSTDVTNSHDINELYLFLKNYLDKTQ"
BASE COUNT          323 a          150 c           92 g          407 t
ORIGIN      
        1 tgaaattata ttattacttc tacaaatttc aaattgccgt aattgaacgt atatttcttc
       61 ttcaactatt atttcatctt tagcataatc tatatataaa attttatgct attatttaaa
      121 taattcgcta taacttaaca tacgttttcg atataaacct tgttctaaat ctcaataatt
      181 ttttgctgtt ttcatcgtca ttagttaaaa aaataattta actgagtttt tgagacctgt
      241 ttaaattgat gactcaaaac ctttatcccc ttttcactcg gtttaattgc tttaatattt
      301 aacacagtct cattgtaatt ttgctactaa gtttgaaata tttcgattcg aatgtgaaac
      361 atcatttatt attactttcg acagctagaa aaatattgtt aaaccaaaac tataattacc
      421 acttttatat agaactatat ataaatttta actttgagac agaactgcta gtcaggttta
      481 tgaatatatt tctttagttt acttgatata cttattggta aatcattatt tatttgaaga
      541 gcatttataa caaaaagtat aatcccagtt atagcgattc catgaccatt tttatcagtc
      601 aaagattcat caacaaaaac tttttgataa atttatatat ttgtataatt tattatggta
      661 gatatctaca cccgtatcta taacagctac agttatcatt gcgtcttatc caaatagttt
      721 ttaagaaata aatataattc attaatatca tggctatttg taacgtcagt gcttaagacc
      781 ttgtcaatta ttgttttatc tgtttctaag agcccctgat tttctgcata catattttgt
      841 atatcgctgt taatctcatt taatttttta aatttttttc taaccttttt caaaataaaa
      901 tgctctttaa ttccatctct taaatacata taaatctcta ttataatctc cataaatgtc
      961 atatctcctc tc
//
LOCUS       DALAGF010000213          971 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.222, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000213 DALAGF010000000
VERSION     DALAGF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.222"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            211..459
                     /locus_tag="O2692_002782"
     CDS             211..459
                     /locus_tag="O2692_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300358.1"
                     /translation="MLILKIKEINDKSVTYKYFPNNDENIKPGIIQMDIDSLEVINAE
                     KSSLEKNTRDNYFIHAIDRIYINTSKGLFPESELVAWG"
     gene            494..616
                     /locus_tag="O2692_002783"
     CDS             494..616
                     /locus_tag="O2692_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300359.1"
                     /translation="MKVIFYCPKRADDILKASMEYQSTDYKRRYISWKTMIAMK"
     gene            592..690
                     /locus_tag="O2692_002784"
                     /pseudo
     CDS             592..690
                     /locus_tag="O2692_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head morphogenesis protein"
BASE COUNT          400 a          109 c          149 g          313 t
ORIGIN      
        1 aaacaatttg atcctgactt ttatatggct caaagttggc aacgtttaag agaagggaaa
       61 aatattaaaa aaatggatat aataatgtag aaacatgaag ctcttgaaca ctatctaatg
      121 aataagtata atttacacta tatagaggca cataaactta cagaaataaa atataattat
      181 agtattttaa ttaattagag gtggaaatga ttgctaatat taaaaataaa agaaattaat
      241 gataaaagtg ttacatataa atattttcct aataatgatg aaaatattaa gcctggtatt
      301 attcaaatgg atatagatag tcttgaagtt atcaatgctg aaaaatctag tttagaaaaa
      361 aatacaagag ataattattt tattcatgcc atagacagaa tatacataaa tactagtaaa
      421 gggctatttc ctgaatctga actcgtagca tggggataaa gaaagtttac tttaaaaatt
      481 ttaaccttca aatgtgaagg ttatttttta ttgcccaaaa cgtgctgatg acattttaaa
      541 agcaagtatg gaatatcagt cgacagacta taaacggagg tatatctcat ggaaaacaat
      601 gatagcaatg aagtagctta tttgttaaaa gatggtaaat ttacaaaagt atatgtcaat
      661 caagatagtg tatcttttgt gccagggtaa caagcaaccg aattattatt taacagtaaa
      721 ccgaattcaa ttgttatgtt acataaccat cctggacagt caggattttc agaatatgat
      781 ttatttacat tttttaagca tccatcaata aaatcaatga caatcgttac taataaggga
      841 caagtaaaat ttattaccaa gtcaaatagg tttcatggta aaatagtgag taaattttgt
      901 gctaaatatt ttacgcatat taatatcatt aatgatagcc atattgaaaa attattgaaa
      961 aaactttata g
//
LOCUS       DALAGF010000214          969 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.223, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000214 DALAGF010000000
VERSION     DALAGF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.223"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..>969
                     /locus_tag="O2692_002785"
     CDS             <1..>969
                     /locus_tag="O2692_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCZ4300360.1"
                     /translation="KSKYAKNIYLNFKKIFSNLEQSVNNYLELEEEFEEKKKYLSEIY
                     ELIDEINIDPYIFLTEHNECNIYKISENEKLELQTYMSKIEKVLLKYQTYLKETCKEC
                     LFYPYLLVQGEAGIGKSHLLAHLSKKLRDENHIIYLFLGQFFTKNEDPWHQILNDLEV
                     TNSVDNFLRSISNKAKETKKRAFIIIDALNEGEGKRLWGNYFQSFINHIKKYSNIALI
                     FSIRTPFEDVILPKNAIQDNNIVVFQHEGFSKEENYNPIVSFCDFYGLELPKLPILNP
                     EFNNPLFLKLMCEYCVNKFKEFDQTISVAELFTNVLKTVNINLSKE"
BASE COUNT          396 a          107 c          142 g          324 t
ORIGIN      
        1 tgaaatcaaa atacgcaaag aatatttatt tgaatttcaa aaaaatattc tctaatttag
       61 aacaatcagt caataattat ttggaactgg aagaagagtt tgaagaaaag aaaaaatact
      121 taagtgaaat ttatgaattg atagacgaaa ttaatattga cccttatatt tttctgacag
      181 aacataatga atgtaatata tacaaaataa gtgagaatga aaaattagaa cttcaaactt
      241 atatgagtaa aatcgaaaaa gtattattaa aatatcaaac gtatttaaaa gaaacatgta
      301 aagaatgctt gttttatcct tatttgctag ttcaaggtga agctggcata ggaaaatcac
      361 accttttagc gcatctatct aaaaaactta gagatgaaaa tcatattatt tatctgtttt
      421 tgggacaatt ttttactaaa aatgaagatc catggcatca aatacttaat gatttagaag
      481 ttacaaattc tgttgataat tttttaaggt caataagtaa taaggccaaa gaaacaaaaa
      541 aaagagcttt tattattatt gatgcgctta atgaaggtga aggtaaaagg ttatggggaa
      601 attattttca aagctttatc aaccatataa agaagtattc taatatagct ttaatatttt
      661 ctattagaac cccttttgaa gatgttatat tacccaaaaa cgcaatacaa gataacaata
      721 ttgtagtatt tcagcatgaa ggttttagta aggaagaaaa ctataatcca attgtatctt
      781 tttgtgattt ttatggatta gagctaccta agttacctat attaaatcca gaattcaaca
      841 atccattatt tttaaaacta atgtgtgagt actgtgtaaa taaatttaag gaatttgacc
      901 aaactataag tgttgcagag ctatttacaa atgttcttaa gacagtaaat attaatttat
      961 ctaaggaag
//
LOCUS       DALAGF010000215          940 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.225, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000215 DALAGF010000000
VERSION     DALAGF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.225"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            224..724
                     /locus_tag="O2692_002786"
     CDS             224..724
                     /locus_tag="O2692_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ4300361.1"
                     /translation="MTFEEKLSEIYNKIANEISGMIPVEWDQVFTIAYVNDRGGEIVF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEDLEPWTSCEF
                     DFTRDGKLNVVFDYVDWMNSEFGPIAKENYYMYKKFGVLPETEYEINKVKEIDQYVKE
                     QDEAEL"
     gene            736..>940
                     /locus_tag="O2692_002787"
     CDS             736..>940
                     /locus_tag="O2692_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ4300362.1"
                     /translation="MTFEEKLSQMYNKIASEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKPGSDELNYYSDIPKDCNVSK"
BASE COUNT          362 a           96 c          188 g          294 t
ORIGIN      
        1 gctattattt acaaataata taatactgca aagactcgaa attatttaga attccagtta
       61 tcgtaacaat agttgcagtt attttagttc cattagtgta tttaggttta aaatgagtaa
      121 tattaatttg aagaagcatt aagcgacgta gaggtgttgt ttagtgcttt ttatttaacg
      181 gaaatgaaac caatatctat tttgaaaata taggggcgat aacatgactt tcgaagaaaa
      241 actaagtgaa atatacaaca agattgcgaa tgagattagt gggatgatac cagtagaatg
      301 ggaccaagta tttacgatag cttatgtaaa tgatagaggt ggagagatcg tttttaatta
      361 tactaaacca ggtagcgatg aattgaatta ttacacatat atccctagag agtataatgt
      421 ctctgaaaaa gtattttatg atttgtggac ggatttatat agattgttta agaagttaag
      481 aaacgcattt aaagaagaag atttagaacc atggacatca tgtgaatttg actttacaag
      541 agatggtaaa ttgaatgttg tatttgatta tgttgattgg atgaattcag aatttggtcc
      601 aatagcaaaa gaaaattatt atatgtacaa aaagtttggt gttttaccag aaacggaata
      661 tgaaattaat aaagttaaag aaatcgatca atatgttaaa gagcaagatg aagctgaact
      721 atagggggag ataacatgac tttcgaagag aaactaagtc aaatgtacaa taaaattgca
      781 agtgagatca gtggaatgat accagtagaa tgggagcaag tatttacaat agcttatgta
      841 actgatcaag ctggggaagt catttttaat tatactaaac caggtagtga tgaattaaat
      901 tattattcag acatacctaa agattgcaat gtctcaaaag
//
LOCUS       DALAGF010000216          900 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.226, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000216 DALAGF010000000
VERSION     DALAGF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.226"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..868
                     /locus_tag="O2692_002788"
     CDS             <1..868
                     /locus_tag="O2692_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4300363.1"
                     /translation="NKRCVLKNISFDIEQGKCIALIGKNGAGKSTLIDILIGNVNANS
                     GEIFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENPLPLEEIIELTKFDS
                     SQLNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEIREYFWSIIENLKED
                     NRTILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQSQITLSDEYIRKLKL
                     DKDDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKVSIVDSYFNNKKQRG
                     SNYDTKLLENRI"
BASE COUNT          369 a          114 c          128 g          289 t
ORIGIN      
        1 taataaaaga tgtgttctaa aaaatatttc gttcgatatt gaacaaggta aatgtatcgc
       61 tttaattgga aaaaatggtg ctggaaagtc aacgttaatt gatatattaa ttggtaatgt
      121 taatgctaat tctggtgaga tatttgataa agacaagtta ttacaaagtg aaaatcgcag
      181 tataatgttc caaaaaacga tgtttccaga tcaattaaaa gttattgaga ttatcaactt
      241 atatcaatca ttttacgaaa atccattacc attggaagaa ataatagaac tgacgaaatt
      301 tgattctagt caactgaacc aatttgtaaa taaactttct ggtggtcaac aacgattact
      361 cgattttgta ttatctttaa tcggacaacc acaattgatc ttattagatg aaccaacatc
      421 gactatggat atagaaatta gagaatattt ttggtcaatt attgaaaatt taaaagaaga
      481 taatcgaacg atactctata catcgcacta tattgaagaa gtcgaacgta tgtcagacaa
      541 aattattctc attgaaaatg gagaaataat acttaatgat tcaacgtcac atattagaac
      601 caatcagcaa tctcagatta cgttatccga tgaatatata agaaagttaa aactagataa
      661 agatgattta gttattcaaa aaaatcataa tggcactatc aaaattatta cttcaaatgt
      721 aaatgatacg attttatatc ttcaacaact tcatattaat ttggatgata ttgaaataca
      781 aaaagtctca attgttgatt catacttcaa caataaaaag caaaggggat ctaattatga
      841 tactaagtta cttgaaaatc gaatttaaag ttataatgcg taaaaaaaca acattaatat
//
LOCUS       DALAGF010000217          880 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.227, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000217 DALAGF010000000
VERSION     DALAGF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.227"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            55..168
                     /locus_tag="O2692_002789"
     CDS             55..168
                     /locus_tag="O2692_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300364.1"
                     /translation="MYFKNSSLNYIPTTFGGEPKETLFYKVSNTLCYVNNS"
     gene            complement(172..255)
                     /locus_tag="O2692_002790"
     tRNA            complement(172..255)
                     /locus_tag="O2692_002790"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(219..221),aa:Leu,seq:caa)
     gene            complement(294..368)
                     /locus_tag="O2692_002791"
     tRNA            complement(294..368)
                     /locus_tag="O2692_002791"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(333..335),aa:Gly,seq:tcc)
     gene            complement(472..546)
                     /locus_tag="O2692_002792"
     tRNA            complement(472..546)
                     /locus_tag="O2692_002792"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(511..513),aa:Gly,seq:tcc)
     gene            complement(554..627)
                     /locus_tag="O2692_002793"
     tRNA            complement(554..627)
                     /locus_tag="O2692_002793"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(593..595),aa:Cys,seq:gca)
     gene            complement(633..704)
                     /locus_tag="O2692_002794"
     tRNA            complement(633..704)
                     /locus_tag="O2692_002794"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(670..672),aa:Gln,seq:ttg)
     gene            complement(716..788)
                     /locus_tag="O2692_002795"
     tRNA            complement(716..788)
                     /locus_tag="O2692_002795"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(753..755),aa:His,seq:gtg)
     gene            complement(791..864)
                     /locus_tag="O2692_002796"
     tRNA            complement(791..864)
                     /locus_tag="O2692_002796"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(830..832),aa:Trp,seq:cca)
BASE COUNT          275 a          197 c          188 g          220 t
ORIGIN      
        1 aagacaacca agcccaataa tggactggcc gcctaataat aaaaactcta aaagttgtat
       61 tttaaaaata gttctttaaa ttatataccc accacatttg gtggagaacc aaaagaaacc
      121 ttgttttaca aggtttctaa tacgttatgt tatgtaaata acagttaatt ataccggtgg
      181 tcggggtcga accgacactc cacaagtgga acgggatttt gagtcccgcg cgtctgccaa
      241 ttccgccaca ccggcttaat ggtaaacaaa aaacttccct ttggaagcaa ttatggagcg
      301 gaagatagga tttacaccta tacctcgttc cgggaaggaa cgtgttctaa aagttgaact
      361 actcccgcaa atattaaatt atggagcgga agataggatt tacacctata cctcattcca
      421 ggaaggaatg tattctaaga gttgaaatac tcccgcatta ttattaaatt atggagcgga
      481 agataggatt tgcacctata cctcgttccg ggaaggaacg tgttctaaaa gttgaactac
      541 tcccgcataa acctggaggc ggcaaccgga tttgaaccgg tgataaaggt tttgcagacc
      601 tctgccttac cacttggcta tgccgccaat aactgggcta gctggattcg aaccaacgag
      661 tgacggagtc aaagtccgtt gccttaccgc ttggctatag cccattaata ataagggcgg
      721 ctgaagggga tcgaaccctc gaatgtcgga accacaatcc gatgtgttaa ccacttcacc
      781 acagccgcca tggcaggggc agtaggaatc gaacccacac caaaggtttt ggagacctct
      841 attctaccgt tgaactatgc ccctattaaa aataataaat
//
LOCUS       DALAGF010000218          849 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.228, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000218 DALAGF010000000
VERSION     DALAGF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.228"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(185..727)
                     /locus_tag="O2692_002797"
     CDS             complement(185..727)
                     /locus_tag="O2692_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HCZ4300365.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLRESGFFRHLKVMPYAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
BASE COUNT          269 a          175 c          108 g          297 t
ORIGIN      
        1 gggcccaccc caacttgcat tgtctgtaga aattggcgtt ccaatttctc tatgttgggg
       61 ccccgcagtc aactactgcc aatatagtgt tgtagtgtct aagacataaa agttcatctc
      121 agtcacaatt actttatagc ctatccatca tcttttctac atgaaatttt tcaagtgata
      181 tattttattt ctcaatatta tctaaaaaat actgttctac atctttccag ctatttacgc
      241 gttcaaatcg atcatcatta atattatgca ctgctgtaaa cataatcggt gtaccagtaa
      301 aaatttcaag ctgtctagga ttgtcatcta ttaaataatc agctttaacg atgttttttc
      361 taccacaaaa aacaaaatgc tgaggatcta aaaatggaaa gaactctagt aaccattcat
      421 atttatcact aaatgatgtt ggtacatcca ttgctgctgt agcaatatat acatcataat
      481 gttcagttaa ttttttcaca acttcttgtg catacggcat cactttaaga tgtctgaaga
      541 agcctgattc tctcaatact tctgtaatta atccatcatg ttcaggaata acatgtttaa
      601 gtttttgacc attcaaagct tccattttaa tacctaaatc cgctctaaaa ttgacagcat
      661 caatgatttc tcctaatgta tctgccaata cttcatccat atcaatcgcg attgatttac
      721 gggtcatttt aatcttctcc tcaaaatatc agtattgatt cagtatagca aaaaagcaat
      781 tgatacaaaa caatatcatc tattgttgct ttttacactg aatactctca cctcatcatt
      841 cacactgca
//
LOCUS       DALAGF010000219          841 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.229, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000219 DALAGF010000000
VERSION     DALAGF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.229"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            180..791
                     /locus_tag="O2692_002798"
     CDS             180..791
                     /locus_tag="O2692_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCZ4300366.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAIVFVIIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAIRNLWNRPLLLKYKNITDSSSGIVNKSE
                     YNSVESLRINIEIAKYKGIISLIVAIVLTVLMTLKSTPQITAETRDLSISFFILSLFI
                     IIIFAVWDLFIRVRKGAFAFVVIRPILFSCWLFILNMILSRLL"
BASE COUNT          291 a           88 c          140 g          322 t
ORIGIN      
        1 atttttgaat taaacactga agatgatgac taaacaattt tataaattct gtgaagtata
       61 cttatatata agataatcaa cttcaacgta tatcaccata ttattttata ctcaatgatg
      121 tgaatggatt taagtggatt gatattaaag taaaagttat ggaatattga ggtgtgaata
      181 tggagttctt attattaatt gtcgtagccg gactgtatta tattatatat ttaactgctg
      241 tgatgtattc tgaaaaaata gtagtattgc ctataatcat ctatgccatt gtgtttgtaa
      301 taattggtat cacttatatc tttataggcg acagctatga tcaattaaca aatttcaatg
      361 tgattttgta tatggggagt ttgttttatg catggatggc tattagaaat ctttggaaca
      421 gaccactatt actaaaatat aagaacatta cagatagttc aagtggaata gttaataaat
      481 ctgaatacaa ttcagttgaa agcttacgta taaatattga aatagctaag tataaaggga
      541 ttatttcttt gatagtagct atagtactaa cggtattaat gacattaaag tcaacacctc
      601 aaatcactgc agaaacacgt gacttaagta tctcattttt catactcagc ttatttatca
      661 ttattatatt tgcagtttgg gatttgttta ttagagttag aaaaggagcg tttgcttttg
      721 ttgtaataag gccaatatta tttagttgtt ggttatttat tttgaatatg attttatcaa
      781 gattattata attgtattac atataggtgc tggtaaattt tatttacgaa atggcaaaaa
      841 a
//
LOCUS       DALAGF010000220          826 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.230, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000220 DALAGF010000000
VERSION     DALAGF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.230"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            191..406
                     /locus_tag="O2692_002799"
     CDS             191..406
                     /locus_tag="O2692_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300367.1"
                     /translation="MNKKSEGLDIIIPRIFRRDHASVESLTENERRLRKEILGSIKKG
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            431..694
                     /locus_tag="O2692_002800"
     CDS             431..694
                     /locus_tag="O2692_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCZ4300368.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKYYRKDNLGVENLYIDYSPTGTLINISKLEEYLIRKHKKWY"
     gene            706..826
                     /locus_tag="O2692_002801"
                     /pseudo
     CDS             706..826
                     /locus_tag="O2692_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 family protein"
BASE COUNT          323 a          123 c          143 g          237 t
ORIGIN      
        1 aatgatgtgt agagttttgc tttcagcatc ttttatatgt tcttgtactt taataacttc
       61 accattaatt aaatgtaaat ctaaagaagt aacgatttct ttatctaaat attgagtgag
      121 caatctctta tgtatactta tcacctcctt aggttgataa caacattata cacgaaaggt
      181 ggaacaacaa atgaacaaaa aatcagaagg gttagacata atcataccaa ggattttcag
      241 aagagatcat gcgtcagtag aatctttaac agaaaatgaa cgtcgactaa gaaaggaaat
      301 attaggaagt attaaaaaag gtgattacag ctacttagaa ataaacaaag ttttctatgc
      361 attagataga gaacttcaat acagagcgaa taataacaaa ctttaacatt tatcgaaagg
      421 agtgatagag atgccaaaaa tcatagtacc accaacacca gaaaacacat atagaggcga
      481 agaaaaattt gtgaaaaagt tatacgcaac acctacacaa atccatcaat tgtttggagt
      541 atgtagaagt acagtataca actggttgaa atattaccgc aaagataatt taggtgtaga
      601 aaatttatac attgattatt caccaacagg cactctgatt aatatttcta aattggaaga
      661 gtatttgatc agaaagcata aaaaatggta ttaggaggat attaaatgag cgacacatat
      721 aaaagctaat taatagcagt actgtgcttc acagtcttag cgattgtgct tatgccattg
      781 ctgtacttca ctacagcatg gtcaattgcg ggattcgcaa gtatcg
//
LOCUS       DALAGF010000221          792 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.231, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000221 DALAGF010000000
VERSION     DALAGF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.231"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..147
                     /locus_tag="O2692_002802"
     CDS             <1..147
                     /locus_tag="O2692_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCZ4300369.1"
                     /translation="GLVEPFIVFYNQENIFYISNAVIDIISVSKDTLDYIKSEFNDHC
                     ASPY"
     gene            complement(207..707)
                     /locus_tag="O2692_002803"
                     /pseudo
     CDS             complement(207..707)
                     /locus_tag="O2692_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532440.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          254 a          133 c          133 g          272 t
ORIGIN      
        1 ggtttagtgg agccatttat tgtattctat aatcaagaaa atatattcta cattagcaat
       61 gctgttatag atatcatcag tgtatccaaa gatactcttg attatataaa gtcagaattc
      121 aatgatcact gtgcttcgcc atactgattt tatagcacag agaaaagccg tagtttcgaa
      181 gagaccaacg gcttttttat aaatctttag attgtcaaat taagctggac aagtttatca
      241 tcttcgatga aactcataaa tacttccaat ggtgttctat aacctaaaga ttttctagga
      301 cgatgattga ttttttgagc aacactgatg atatgatcgt ctgttactaa attgaaatct
      361 agttctttat ctaatccact gcgtctaagt attccgttag aatgttcatt taaacctcgc
      421 tgtgatggcg tgcctggatc agcaaaaaat atagatatat cgtgacagtt acataatgtt
      481 ttccagtttg agaactcctt accacaatca aaagtaattg atttaaatat atgagaaggt
      541 aaagatgaaa acattgagtg tagagcgttt tctatatcag ttgccttacg acctaagggt
      601 cgtaaggtga taatcatctt ggatactctt tctaccaaag taatgattgc actaccatga
      661 cgacgaccaa caatagtatc tccttcaaga tgtccaaact ccttatgttt tctttggtag
      721 gaaatacata ttgagtttag cacataaggc ttatttattt acctttttct tcacgacgtt
      781 ttaaataata gg
//
LOCUS       DALAGF010000222          791 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.232, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000222 DALAGF010000000
VERSION     DALAGF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.232"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(369..791)
                     /locus_tag="O2692_002804"
                     /pseudo
     CDS             complement(369..791)
                     /locus_tag="O2692_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          284 a          122 c           81 g          304 t
ORIGIN      
        1 taagataata gtacataaaa caaatcactc tattacatat taattttata atttatatac
       61 aaagaaaata tatcaatatt accattaaat tgcaacaatt ctacatatat ttcttcaaaa
      121 aagatacgca ttgaattttt attacatata actctcatga aaattgatta aactaaatta
      181 attagttagc cttaaattta acaaattaaa tcaaaccgaa tgaggctaaa ttggttattt
      241 ttcaagatat gatttgtata aatcaaaaca tttcttacaa atcaaatgtg tataaaatgt
      301 gatatacatt ttcaacctta aaaaagttct aaaaaaagat gagcatctac tgtctcatct
      361 ttcagttctt ttttacgggt ctgttttcta atttgagcac aatcttcgat ttctttatct
      421 aaatgactac caattaaatc tatttcttct attgttaaat cgctatctcc atcttctttt
      481 atctctggta ttattttttc ttcaactaag tcacgatata gtgtttttga attctcgttc
      541 aatttcgatt cgtgattttg aatactttct tccgcaccaa tgtatatcta ttggcactag
      601 cttctacttt tgtaccatca ataaaaattg aattatcatc aataagattt tgctttaaac
      661 attgactata gaactgaata aataaagatt caattaacgc atcagtatta ggattcactc
      721 taaaacgatt aatagtttta taagaaagtg tttgatcttg ggctaaccac atcattcgaa
      781 tactgtcatg a
//
LOCUS       DALAGF010000223          779 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.233, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000223 DALAGF010000000
VERSION     DALAGF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.233"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..402
                     /locus_tag="O2692_002805"
     CDS             <1..402
                     /locus_tag="O2692_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCZ4300370.1"
                     /translation="QNYGLIDNLTVNENLDIGLAYKKISKKEKEQIKIRYIEQFGLSN
                     SLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPTGALDPKTGQMIIQSLFGLVDEN
                     KVLILATHDMAIANQCDEIIDLEQYRKVASM"
     gene            complement(490..756)
                     /locus_tag="O2692_002806"
     CDS             complement(490..756)
                     /locus_tag="O2692_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300371.1"
                     /translation="MKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTASALWFFPG
                     AIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
BASE COUNT          328 a           98 c          131 g          222 t
ORIGIN      
        1 caaaattatg gattaatcga taatttgaca gtaaatgaaa atttagatat tggattagca
       61 tataaaaaaa taagtaagaa agaaaaagaa caaataaaga tacgttatat agaacagttt
      121 ggtctgtcaa acagtttaaa aagaaaagtt catacgctaa gtggaggtga acaacaacgt
      181 gtcgctttaa ttagaatgat gttaaaagat ccgattgtta tgttagctga tgaaccaacg
      241 ggtgcgttag atcctaaaac aggacagatg attattcaat cattatttgg tttggtcgat
      301 gaaaataaag tgctgatttt agcaacacat gatatggcta ttgcaaatca atgtgacgaa
      361 ataatagatt tagaacagta tagaaaagta gcatctatgt gatatccaaa agataaaatt
      421 aaaagccaat caatgggaaa tgttgacttc aacactccgt ttgattggct tttatactgt
      481 gttactaagt tatgaaagta acaacggcaa actgaaaaaa agcaatataa taatatttaa
      541 tacgattagt aataaatgta attgattttt cgttttaata tagcaaaaaa caatgactaa
      601 agataacaac aatatcatga ttgcacctgg gaagaaccat aatgctgaag ctgtgtacca
      661 aatcattggt aacgccataa taataaaaga tataagacaa gaaaggatac caataatatt
      721 ataagttcta gtcaaaatta cacctccaat tttcacttta aaatgacatg cgtaaaaca
//
LOCUS       DALAGF010000224          761 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.234, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000224 DALAGF010000000
VERSION     DALAGF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.234"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..208
                     /locus_tag="O2692_002807"
     CDS             <1..208
                     /locus_tag="O2692_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300372.1"
                     /translation="NIGQNSGNINHLISKELRDKYEWNPSLLSKIDNYLKPAIEIKEH
                     NFSDNSLNINVTFNALYIIKIGKK"
     gene            354..713
                     /gene="sarX"
                     /locus_tag="O2692_002808"
     CDS             354..713
                     /gene="sarX"
                     /locus_tag="O2692_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HCZ4300373.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEKERLSKVRSSKDERKIYIYLNNDD
                     ISKFNALFEDVEQFLNI"
BASE COUNT          313 a           77 c          117 g          254 t
ORIGIN      
        1 aaacattgga caaaatagtg gcaacattaa tcatttgatt tctaaagaat taagagataa
       61 atatgaatgg aaccctagtt tactatctaa aattgacaac taccttaaac cagcaataga
      121 gattaaagag cataatttta gtgataattc cttgaatatt aacgttactt ttaatgcgtt
      181 atacataatt aaaataggaa aaaaataaca ccttgatatg tattgcaaaa tttaatttgc
      241 atttgttggt aattttgaca acgtattaaa aacaaatgag aaagacaatg gctgaaaaat
      301 ttaagataat aatgacagaa gcattgtctt tatatatttg ggggtgcaac attttgaata
      361 ctgagaaatt agaaacattg cttggcttct ataaacaata taaagcatta tctgaatata
      421 ttgataaaaa atataagttg tcgctaaatg atttagcagt cttagattta acgatgaagc
      481 attgcaaaga tgaaaaagta cttatgcaat catttttaaa aactgcaatg gatgagctag
      541 atttaagtag gacaaaatta ttagtttcta taagaagact aattgaaaaa gaaagactta
      601 gtaaagttag atcatctaaa gatgagcgta aaatttatat ttatttaaat aatgatgata
      661 tatctaaatt taatgcttta tttgaagatg tagaacaatt tttaaatatt taattgaaat
      721 tgagtgtcga aagcatagaa tttgcttatc ggcacatttt t
//
LOCUS       DALAGF010000225          754 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.235, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000225 DALAGF010000000
VERSION     DALAGF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.235"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            6..81
                     /locus_tag="O2692_002809"
     tRNA            6..81
                     /locus_tag="O2692_002809"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:tgt)
     gene            88..160
                     /locus_tag="O2692_002810"
     tRNA            88..160
                     /locus_tag="O2692_002810"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            194..268
                     /locus_tag="O2692_002811"
     tRNA            194..268
                     /locus_tag="O2692_002811"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:226..228,aa:Gly,seq:gcc)
     gene            276..364
                     /locus_tag="O2692_002812"
     tRNA            276..364
                     /locus_tag="O2692_002812"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Leu,seq:taa)
     gene            370..446
                     /locus_tag="O2692_002813"
     tRNA            370..446
                     /locus_tag="O2692_002813"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:404..406,aa:Arg,seq:acg)
     gene            467..540
                     /locus_tag="O2692_002814"
     tRNA            467..540
                     /locus_tag="O2692_002814"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:501..503,aa:Pro,seq:tgg)
     gene            564..639
                     /locus_tag="O2692_002815"
     tRNA            564..639
                     /locus_tag="O2692_002815"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:597..599,aa:Ala,seq:tgc)
BASE COUNT          192 a          162 c          183 g          217 t
ORIGIN      
        1 atatagccgg cctagctcaa ttggtagagc aactgacttg taatcagtag gttgggggtt
       61 caagtcctct ggccggcacc atcttttgag ccattagctc agctggtaga gcatctgact
      121 tttaatcaga gggtcagagg ttcgaatcct ctatggctca ttacgattta atttttatat
      181 ttagcaaaat aatgcagaag tagttcagcg gtagaataca accttgccaa ggttggggtc
      241 gcgggttcga atcccgtctt ctgctccatt attttgccgg ggtggcggaa ctggcagacg
      301 cacaggactt aaaatcctgc ggtgagagat caccgtaccg gttcgattcc ggtcctcggc
      361 accattttag cgcccgtagc tcaattggat agagcgtttg actacggatc aagaggttat
      421 gggttcgact cctatcgggc gcgccatttt taaattaatt gaataacggg aagtagctca
      481 gcttggtaga gcacttggtt tgggaccaag gggtcgcagg ttcgaatcct gtcttcccga
      541 ttacttctta aattccattt tatgggggct tagctcagct gggagagcgc ctgctttgca
      601 cgcaggaggt cagcggttcg atcccgctag tctccaccat ttatttttta cacgatgaac
      661 attgaaaact gaatgacaat atgtcaacgt taattccaaa aaacgtaact ataagttaca
      721 aacattattt agtatttatg agctaatcaa acat
//
LOCUS       DALAGF010000226          745 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.236, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000226 DALAGF010000000
VERSION     DALAGF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.236"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..213
                     /locus_tag="O2692_002816"
     CDS             <1..213
                     /locus_tag="O2692_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ4300374.1"
                     /translation="IPTFKNICLSLFAYILFGGLSTMALSMLVTSSPQNIIEFLALIG
                     MTACFFLLNMSSVLDKKIHVILKTNK"
     gene            270..386
                     /locus_tag="O2692_002817"
     CDS             270..386
                     /locus_tag="O2692_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300375.1"
                     /translation="MILNRGAILEQGNKTAKVIKSELITNYIMLATLQIKNN"
     gene            409..>745
                     /locus_tag="O2692_002818"
     CDS             409..>745
                     /locus_tag="O2692_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCZ4300376.1"
                     /translation="MLCESKIINKNPKYRIIKYNDEYLMIDIISTWISLFFPFINWFI
                     PKRYVKISREEFENLNIVKPAKKNVFWPVAGISTLFAVTLRKYTHLLDTQLDRKLVIA
                     ICCITFIGIL"
BASE COUNT          278 a           94 c          109 g          264 t
ORIGIN      
        1 atacctacat ttaaaaatat ttgtttatcc ttatttgctt atatattatt tggtggattg
       61 tcaacaatgg ctctgagtat gttagtaact tcatcccctc aaaatataat agaatttctt
      121 gctttaattg gcatgactgc atgcttcttt ctactgaata tgtcatcggt tctagataaa
      181 aaaattcatg ttattttaaa aacaaataag tagtaaaatt gattaactta ggtagtatcg
      241 gatacttaaa tgttggttca taaaaagcaa tgattttaaa tcgaggagct atcttagaac
      301 agggaaataa aacagccaaa gttataaaaa gtgaattaat aactaattat attatgttag
      361 ccacgcttca aataaaaaat aattagaata aggtgggatt gataatcaat gctatgcgaa
      421 tctaaaatca tcaataaaaa ccccaaatat agaattatta aatataatga tgagtactta
      481 atgatcgata taataagcac ttggattagt ttattttttc cttttattaa ttggttcatc
      541 cccaaaagat acgtcaaaat tagtagagaa gagtttgaaa atttaaatat tgtgaaacct
      601 gctaaaaaga atgttttttg gccagttgca ggtatctcta ctttattcgc agttacatta
      661 agaaagtata cacatttact tgacactcaa cttgatagaa aattagttat tgctatatgt
      721 tgtatcacat ttatagggat tttaa
//
LOCUS       DALAGF010000227          740 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.237, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000227 DALAGF010000000
VERSION     DALAGF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.237"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            8..523
                     /locus_tag="O2692_002819"
     CDS             8..523
                     /locus_tag="O2692_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HCZ4300377.1"
                     /translation="MKIKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVIELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDQDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
BASE COUNT          290 a           85 c          125 g          240 t
ORIGIN      
        1 gagcaacatg aaaataaaaa ctgaatttaa agggaacaat ataccatatg aatacgcagc
       61 aggtgcagat gtgagtgatt ctattaacgg gaatccaatt aagtcatttc catttgaagt
      121 aattgaatta ccggaaggga ctaaatatct tgcttggtct ttaattgact atgatgcaat
      181 tcctgtatgt ggctttgctt ggattcattg gagtgtagct aatgtaagtg ttagtggcaa
      241 ttcaatttct ataaaagcag atttatcaag aacaaagggc gactatgtac aaggtaaaaa
      301 tagctttact agtgggttgt tggctgaaga tttttcagaa atagaaaatc actatgtagg
      361 acctacacca cctgatcaag atcatcaata tgaattaaca gtttatgcgt tagatcattc
      421 tttaaatttg aagaatgggt tctacttgaa tgaattttta aaagaagtaa atcaacataa
      481 aattgatcaa acaagtatta accttatagg aagaaaaatt taatactaaa tatctcatca
      541 atataaaatt gttcaattaa aagtacaaag aaacaaaggt tttaatttat atattaggta
      601 cggcgttcgc tataatgcaa agaagtaatt aaatttaaga aatgtaaact tagttattgt
      661 aatgtgaatt tatttgaaaa aatagaaagt attaacaatt atagctttta cattaattaa
      721 aatttatttt taaaaacaag
//
LOCUS       DALAGF010000228          729 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.238, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000228 DALAGF010000000
VERSION     DALAGF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.238"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            14..541
                     /gene="isaB"
                     /locus_tag="O2692_002820"
     CDS             14..541
                     /gene="isaB"
                     /locus_tag="O2692_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HCZ4300378.1"
                     /translation="MNKTSKVCVAATLALGTLIGVTVVENSAPTSKQAQAAITPYYTY
                     NGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEA
                     SQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
BASE COUNT          288 a           84 c          131 g          226 t
ORIGIN      
        1 aagagagata attatgaata aaaccagtaa agtttgtgta gcagcaacat tagcattggg
       61 cacactgatt ggagtaacag ttgttgaaaa ttcggcgcca acaagtaaac aggcacaggc
      121 agcaataacc ccatattata cttataatgg ttatattggt aataatgcta attttattct
      181 ggataagaat tttattaacg cgattaagta tgataatgtg aaattcaatg gtattaaatt
      241 agctaaaacg aatacgataa aaaaagtaga gaaatatgat caaactttta aaggtgtttc
      301 tgcaaaagga aacgaagcaa gtcaattgca atttgtagtt aaaaataata tttcattaaa
      361 agatatccaa aaagcttatg gcaaggactt gaaaaaagaa aatggtaaaa caaaggaagc
      421 tgatagcggt attttttact atcaaaatgc taaaaagaca ttaggcatct ggtttgtcgt
      481 tgatcataat agagttgtcg aagtaacagt tggacataca ccatacaaaa caagtaaata
      541 aaataatggc atattaaggc tagagtgtga ggagtgatac cgcattctag tcttttttat
      601 taaataataa cgattattgc gtcttacata gttgtttgaa attgataaaa atgtttattt
      661 gattatgatt atttaaatag ttatctcaca tatttcaagc attgattaag tatatagagc
      721 actattttg
//
LOCUS       DALAGF010000229          709 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.239, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000229 DALAGF010000000
VERSION     DALAGF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.239"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            36..254
                     /locus_tag="O2692_002821"
     CDS             36..254
                     /locus_tag="O2692_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300379.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLQIAIIVTIIAFVLNLLAYLGLR"
     gene            386..>709
                     /locus_tag="O2692_002822"
     CDS             386..>709
                     /locus_tag="O2692_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ4300380.1"
                     /translation="MTFEEKLSEMYSEIANKISSMIPVEWEKVYAMAYIDDGGGEVFY
                     YYTEPGSNELYYYTSVLNKYDISESEFMDSVYELYKQFQNLRNLFKEEGHEPWTSCEF
                     DFTREG"
BASE COUNT          276 a           67 c          148 g          218 t
ORIGIN      
        1 attgaaagac aaaataatga gtaggagtta atgagatgga aaaccaaaaa caaggcaatg
       61 gcttaaaaat tgcaacatgg gtatttattg tattaacagt agttacaccg ctatttggta
      121 ttggaagtat tgtttgtagt attaattata aaaaatacga tgctgaaaaa ggttcgaagt
      181 tattgcaaat tgcaattatc gtaacaataa ttgcttttgt tttaaattta ttagcatatt
      241 taggtttaag ataagtaaaa cgaatttgaa gaagcattaa gcgacatttg ggtgttgttt
      301 aatgcttttt atttaacgga aataagtgta gagaataaat taatagttta atcaagagat
      361 atttcgaaca cataggggcg ataacatgac tttcgaagaa aaattaagtg aaatgtatag
      421 cgagattgcg aataagatta gcagcatgat accggtagag tgggaaaagg tatatgcaat
      481 ggcatatata gatgacggag gaggagaagt gttctactat tacacagaac ctggaagtaa
      541 tgaattatac tactatacta gtgtattaaa taaatatgat atatcggaat cggaatttat
      601 ggattcagtg tatgagttgt ataaacaatt tcaaaattta aggaatttat ttaaagaaga
      661 aggacatgaa ccatggacat catgcgaatt tgactttaca agagaaggt
//
LOCUS       DALAGF010000230          708 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.240, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000230 DALAGF010000000
VERSION     DALAGF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.240"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            123..>708
                     /locus_tag="O2692_002823"
     CDS             123..>708
                     /locus_tag="O2692_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HCZ4300381.1"
                     /translation="MYKNYNLIQLTLPIETSVRIPQIDISRYVNEIVETIPDSEFDKF
                     RHHRGATSYHPKMMLKITLYAYTQSVFSGRRIEKLLHDSIRMMWLAQNQTPSYKTINR
                     IRVNPNTDALIESLFIQFHSQCLKQNLIDGTKVEANANRYTFVWKKSIQNHESKLNEN
                     SKTLYRDLVEEKIIPEIKEDGDSDLTIEEIDLIGS"
BASE COUNT          276 a          103 c          105 g          224 t
ORIGIN      
        1 aaaaaataag acactttgcc aaacttgcac ataaatgttt aattcaataa tttgaatttt
       61 ctgtgttggg tcccttcgta taatttaata aataccacta aactaaatta atgaagtgcc
      121 ttatgtataa aaattataac ttgatccaac ttacactacc aatagaaact tctgttagaa
      181 ttcctcaaat tgatatttcg cgatatgtta atgaaattgt tgaaacgata cctgatagcg
      241 aattcgataa attcagacat catcgtggcg caacatccta tcatccaaaa atgatgttaa
      301 aaatcacctt atatgcatat actcaatctg tattttctgg tcgtagaata gagaaattac
      361 ttcatgacag tattcgaatg atgtggttag ctcaaaatca aacaccttct tataaaacta
      421 ttaatcgtat tagagtgaat cctaatactg atgcgttaat tgaatcttta tttatccagt
      481 ttcatagtca atgtttaaag caaaatctta ttgatggtac aaaagtagaa gctaatgcca
      541 ataggtatac atttgtgtgg aagaaaagta ttcaaaatca cgaatcgaaa ttgaacgaaa
      601 attcaaaaac attatatcgt gacttagttg aagaaaaaat aataccagag ataaaagaag
      661 atggagatag cgatttaaca atagaagaaa tagatttaat tggtagtc
//
LOCUS       DALAGF010000231          702 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.241, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000231 DALAGF010000000
VERSION     DALAGF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.241"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            199..>702
                     /locus_tag="O2692_002824"
     CDS             199..>702
                     /locus_tag="O2692_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCZ4300382.1"
                     /translation="MTTKENIDTLRKPGAQALSLISLFLILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIVISLLQWIRFIDFEKESAQKYKKIYARFLVVINVLTTITVVFALC
                     NLYYFAAVQNHYDLFNYWLMGSISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTHF
                     GLLLTALS"
BASE COUNT          254 a           75 c          106 g          267 t
ORIGIN      
        1 atgtaagact aaacaataaa gctttgttta gtctttttag cgtttaagta aaaagcaata
       61 gataccgtaa agttgatgct catcaaataa taatataaag ataattttag gtttttaaac
      121 ttttaatcga aaagaatcta agtttatagc tttaaatacg taatatgcta tttaaaaaat
      181 aatgtatagg agatagatat gactactaaa gaaaatattg atactcttcg aaagccaggc
      241 gcacaagcct taagtttaat atcattgttt ttgatacttt tttcatgttt gactttcttt
      301 tttggtttag attatgaaag gtttccaaat tatttaaaga taacgacaat tatagaatta
      361 ataattattg taattagttt acttcaatgg attagattta tagatttcga aaaagaaagt
      421 gcacagaaat ataaaaaaat atatgctcga tttttagttg ttataaatgt gttaactact
      481 atcactgtag tatttgcact gtgtaatctt tattattttg cagctgtaca aaaccattat
      541 gatttattta attattggtt gatgggttcg atttcaatca taattagcta tttattatta
      601 gtaattggcg gaatgttcac gttattaaaa ttacctaaag taacaaaacg ttggggtggt
      661 aaaactaaaa cacatttcgg tttattatta actgcgttga gc
//
LOCUS       DALAGF010000232          673 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.242, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000232 DALAGF010000000
VERSION     DALAGF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.242"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..592)
                     /locus_tag="O2692_002825"
     CDS             complement(<1..592)
                     /locus_tag="O2692_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCZ4300383.1"
                     /translation="METLLCESKVINKNPKYRIIKYDSEYLMIDLASNWIVFFFPFIN
                     WLIPKTYVKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEEL
                     IVWSSCFIGFLEIIFFYCYLNKKLTLNIYNESKNNELKLRLLPSFKNICFTIFYYLFT
                     GFMSYGAFYLLVFENVQNLILYVSWLFMTMLFMFMNM"
BASE COUNT          263 a          103 c           74 g          233 t
ORIGIN      
        1 gcatattcat aaacataaat agcatagtca tgaaaagcca agaaacatat aagattaaat
       61 tttgcacatt ttcaaatacc aacaagtaaa atgccccata agacatgaaa ccagtaaata
      121 ggtaataaaa aattgtgaaa caaatatttt taaaggaggg taataatctt aatttaagtt
      181 cattattttt actttcatta taaatattta atgttaattt cttatttaga taacaataaa
      241 aaaatataat ctctaaaaac ccaatgaaac agctgctcca aacaattagt tcttctaatt
      301 gaaaatcaaa taaataagta tttcttctta cagtaccacc aagtaacaca atacccgcga
      361 atatggtcca tcctatcgat ttatttttaa ctggtttgac aatatttaat ttttcataat
      421 cattcttagt gattttgaca tatgttttcg gtatgagcca gttaataaat ggaaagaaga
      481 agacaatcca attacttgcc aaatcaatca ttaaatattc actatcgtat ttgattattc
      541 gatatttagg gtttttatta ataactttag attcgcaaag caatgtctcc acatcccttt
      601 aattttatgt gtaatacatt tttcgatact tcaaaagaca ttcaaatact atcaagttac
      661 tgtcatcaaa ggt
//
LOCUS       DALAGF010000233          625 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.243, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000233 DALAGF010000000
VERSION     DALAGF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.243"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..>625)
                     /locus_tag="O2692_002826"
     rRNA            complement(<1..>625)
                     /locus_tag="O2692_002826"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          130 a          191 c          129 g          175 t
ORIGIN      
        1 tcgtttacgg cgtggactac cagggtatct aatcctgttt gatccccacg ctttcgcaca
       61 tcagcgtcag ttacagacca gaaagtcgcc ttcgccactg gtgttcctcc atatctctgc
      121 gcatttcacc gctacacatg gaattccact ttcctcttct gcactcaagt tttccagttt
      181 ccaatgaccc tccacggttg agccgtgggc tttcacatca gacttaaaaa accgcctacg
      241 cgcgctttac gcccaataat tccggataac gcttgccacc tacgtattac cgcggctgct
      301 ggcacgtagt tagccgtggc tttctgatta ggtaccgtca agatgtgcac agttacttac
      361 acatatgttc ttccctaata acagagtttt acgatccgaa gaccttcatc actcacgcgg
      421 cgttgctccg tcaggctttc gcccattgcg gaagattccc tactgctgcc tcccgtagga
      481 gtctggaccg tgtctcagtt ccagtgtggc cgatcaccct ctcaggtcgg ctatgcatcg
      541 ttgccttggt aagccgttac cttaccaact agctaatgca gcgcggatcc atctataagt
      601 gacagcaaga ccgtctttca ctttt
//
LOCUS       DALAGF010000234          578 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.244, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000234 DALAGF010000000
VERSION     DALAGF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.244"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
BASE COUNT          241 a           87 c           69 g          181 t
ORIGIN      
        1 taaaaactaa agtaaaaatg ttggatgtga aagattgttg atgagaattt tgagatgtag
       61 ataaatatca aactagtcta taggtaaaaa tttataatta atttgaaaag aatgtctcaa
      121 ataatgtttg aattagcagg tgtcatacta agtaatgtta agtaaacatt ttatacttta
      181 tcagaacaaa aaagacatgc atcaacatgt cagtgaaact aatccatatt atcttaacaa
      241 cgaaattgaa tcgaacctga aaaacgtatt tacaaatcgc aactactcta tccaaggcaa
      301 aatcgcaaaa tctgatcccc gaaataacat atctcgaaaa aaattccata attcaatcat
      361 cttcaatatt taacaataca ttactagttc caaatatact catacataca tgtttcattg
      421 attgactgca tattgtcata aataataggg gaactaatcg ctaataatgt tgttaaacac
      481 aatacatagt aaagtgaaag attataaata catgaattgt tcaccattcc gccatatatt
      541 ttcaatcacc taatttccga ataaatatta caatttta
//
LOCUS       DALAGF010000235          554 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.245, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000235 DALAGF010000000
VERSION     DALAGF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.245"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..>554)
                     /locus_tag="O2692_002827"
     CDS             complement(<1..>554)
                     /locus_tag="O2692_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300384.1"
                     /translation="LLLYQNQYLILVVAQILLGLSYSMMAGVDTAIIKRNITNEKYVQ
                     NKSNSYMFLSLLISGIIGSYLYGINIKWPIIMTGIFSILTIIIIRCTLVENRELNLIG
                     ETKGKIKKFLPEEKFWILHYSFLRALILGFFIGFIPINIYNDLKLNNLQFISVLTCYT
                     VMGFVSSRYLTKYLNYKFVSEICLV"
BASE COUNT          211 a           84 c           50 g          209 t
ORIGIN      
        1 actaaacaaa tttctgacac aaacttataa ttcaagtatt tagttaaata acgtgaagat
       61 acaaaaccca taactgtgta acaagttaat actgaaataa attgtaaatt attcagtttt
      121 aaatcattat atatattaat tggaataaat cctataaaaa atcctaatat taacgctctt
      181 aaaaaagaat aatgcaatat ccaaaacttc tcttctggta gaaatttctt tatctttccc
      241 tttgtttctc ctattaaatt taattcccta ttttcaacta atgtgcatcg aataattata
      301 attgttagaa ttgaaaatat accagtcatt attataggcc atttaatatt tattccataa
      361 agataactac ctataatccc tgaaattaat aatgatagga acatatagct atttgactta
      421 ttttgtacgt atttctcatt tgttatattt cttttaatta ttgcggtatc aacacccgcc
      481 atcattgagt aacttaaccc taataatatt tgtgccacta ctaaaattaa atattgattt
      541 tgatataata acaa
//
LOCUS       DALAGF010000236          549 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.246, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000236 DALAGF010000000
VERSION     DALAGF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.246"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..78
                     /locus_tag="O2692_002828"
     CDS             <1..78
                     /locus_tag="O2692_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCZ4300385.1"
                     /translation="LKNKIFVFEQSIKKHNEMMYIELCD"
     gene            128..520
                     /locus_tag="O2692_002829"
     CDS             128..520
                     /locus_tag="O2692_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300386.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKENYNKYIQGQKQRVIFENGHFV"
BASE COUNT          224 a           41 c           97 g          187 t
ORIGIN      
        1 ttaaagaata aaatatttgt ttttgaacaa agtattaaaa aacataacga aatgatgtat
       61 attgaactat gtgattgaaa aaatttttaa ttaaggcttg ttaaaactta agagaggatg
      121 tttttaaatg caattcaaat taaaagaaga agagattatt agttttttag aattgaaata
      181 tccagaaaaa gagttcgaat atggtcgttt gttagttgga caacataaac gtgatgattt
      241 agatgtttat tactttggtg atacgttttt aatgtgcacg attatttcat tcaagacatt
      301 tgaaattaaa gaaacagtag aattatcata tgatgctgtt aatcgtattg tgttaaaaga
      361 tggatggtta tttagaaaaa tgagaataga aacaatgcaa aaagtgttaa aatacggtac
      421 atctaaatta atgttaactg attttcaaaa agagaattat aataaatata ttcaaggtca
      481 gaaacaacgc gtgatatttg aaaatggcca ttttgtctaa ttgatagtga atataattag
      541 agtaagagg
//
LOCUS       DALAGF010000237          541 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.247, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000237 DALAGF010000000
VERSION     DALAGF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.247"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
BASE COUNT          146 a          124 c           79 g          192 t
ORIGIN      
        1 ttcgatttaa aagatattag acataaaatc taaaaacagc agtaagatga tttatgatta
       61 aaaactatct tactgctgtt cactttttat aatacttctg aatgtcttca cttatacttc
      121 tagtcacaga tttaaataat caaaagtgca cattattaaa atatcaattt cacactcaat
      181 gcggctcatc gcattcattt cttgtctagc aacgttctac tctagcggaa cgtaagttag
      241 ctaccatcct cgctaagaac ctttcttgac ttgtgacaat cgcttgcttc tttcctctcc
      301 ttcggctctc gcttactcat ttagctctac taaactcgtt gcgctctttt ctcgtttcgt
      361 cagattcaaa cgttttcact tcgccaagcc atttttcttt gtgtttactt tttattttga
      421 cgttttagac ataaaaaaag agacctcacg gtctcaactt gcctggcaac gttctactct
      481 agcggaacgt aagttcgact accatcgacg ctaaggagct taacttctgt gttcggcatg
      541 g
//
LOCUS       DALAGF010000238          530 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.248, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000238 DALAGF010000000
VERSION     DALAGF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.248"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
BASE COUNT          139 a          115 c           80 g          196 t
ORIGIN      
        1 ttcaataaag tatataaaag agcagtaaga tcattttcaa ttagataccg tcttactgct
       61 cttctctatt tatacaatat tttgttttcg atgatatcat tttaaattta tataaacaca
      121 aaattaaagt tgtacatact tagacaatcg aaagtgtaca ttattaaatt atcatttcca
      181 gttctactct agcggaacgt aagttggcta ccatcctcgc taagaacctt tcttgacttg
      241 tgacaatcgc ttgcttcttt cctctccttc ggctctcact tactcattta gctctactaa
      301 actcgttgcg ttcttttctc gtttcgtcag attcaaacgt tttcacttcg ccaagccatt
      361 tttctttgtg tttgcttttt attttgacgt tttagacata aaaaaaagag accttgcggt
      421 ctcaatgcgg ctcatcgcat ccattttttg cctggcaacg ttctactcta gcggaacgta
      481 agttcgacta ccatcgacgc taaggagctt aacttctgtg ttcggcatgg
//
LOCUS       DALAGF010000239          528 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.249, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000239 DALAGF010000000
VERSION     DALAGF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.249"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            4..79
                     /locus_tag="O2692_002830"
     tRNA            4..79
                     /locus_tag="O2692_002830"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Lys,seq:ttt)
     gene            83..164
                     /locus_tag="O2692_002831"
     tRNA            83..164
                     /locus_tag="O2692_002831"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:117..119,aa:Leu,seq:tag)
     gene            168..242
                     /locus_tag="O2692_002832"
     tRNA            168..242
                     /locus_tag="O2692_002832"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:200..202,aa:Gly,seq:gcc)
     gene            253..341
                     /locus_tag="O2692_002833"
     tRNA            253..341
                     /locus_tag="O2692_002833"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:287..289,aa:Leu,seq:taa)
     gene            360..433
                     /locus_tag="O2692_002834"
     tRNA            360..433
                     /locus_tag="O2692_002834"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:394..396,aa:Arg,seq:acg)
     gene            443..516
                     /locus_tag="O2692_002835"
     tRNA            443..516
                     /locus_tag="O2692_002835"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:477..479,aa:Pro,seq:tgg)
BASE COUNT          118 a          122 c          150 g          138 t
ORIGIN      
        1 gaagagccat tagctcagtt ggtagagcat ctgactttta atcagagggt cagaggttcg
       61 aatcctctat ggctcaccat ttgcgggtgt ggcggaattg gcagacgcac tagacttagg
      121 atctagcgcc ttacggcgtg ggggttcgac tcccttcacc cgcatatgca gaagtagttc
      181 agcggtagaa tacaaccttg ccaaggttgg ggtcgcgggt tcgaatcccg tcttctgctc
      241 catttttata gtgccggggt ggcggaactg gcagacgcac aggacttaaa atcctgcggt
      301 gagtgatcac cgtaccggtt cgattccggt cctcggcacc attttcaata aaaacatatg
      361 cgcccgtagc tcaattggat agagcgtttg actacggatc aagaggttat gggttcgact
      421 cctatcgggc gcgttaatta tacgggaagt agctcagctt ggtagagcac ttggtttggg
      481 accaaggggt cgcaggttcg aatcctgtct tcccgatata ctgtaatt
//
LOCUS       DALAGF010000240          514 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.250, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000240 DALAGF010000000
VERSION     DALAGF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.250"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
BASE COUNT          207 a           50 c           76 g          181 t
ORIGIN      
        1 tctcattcaa taattaatat gatcgaaagt ttcatgaaaa tatattagtc gattttgaaa
       61 gaagcgtagt caatttatgt ctttctataa aaatttacta ttaaaaaaat ctctgcgtat
      121 ttttctataa tcagtttcgt atttatacga ttctggagtt caatattaat gtcttatatt
      181 taaaatgcca cacctatgca atagaatata tattcttttt gtgagtagaa aatttgttat
      241 attagaagcg aataatttta aaacatatta tgaaatgtaa gaagaaatat tatgaaacta
      301 ggaaaagtag attgttgaaa attccataca ataggagaag taaagtgaat aaattctggt
      361 attactaaag ctattgattg tcctaattgt aataaaaagt tggatagtgt agttgagggg
      421 agaaaaacta aaatgcaaaa atatattctc aatggatcat tataaatacc aattagatta
      481 aagtattgcg aacatttaca attagaaatc agta
//
LOCUS       DALAGF010000241          505 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.251, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000241 DALAGF010000000
VERSION     DALAGF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.251"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            114..>505
                     /gene="lpl8"
                     /locus_tag="O2692_002836"
     CDS             114..>505
                     /gene="lpl8"
                     /locus_tag="O2692_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HCZ4300387.1"
                     /translation="MGYIKRMALYMSVFLLIIFIVGCRNMKDEQKKEEQTNKTDSKEE
                     QIKKSFEKTLDMYPIKNLEELYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNE
                     GMVLYLDRNTRTAKGHYFVKTFYNKGKFP"
BASE COUNT          214 a           47 c          100 g          144 t
ORIGIN      
        1 aattcatata gtcagttttg tttgatgaca tgatagtaga aaaattatta ttttaatagg
       61 ttgtatgaac tatacaaata tttataaaaa ttaaaacaga gagatgtgaa atgatggggt
      121 atataaaaag aatggcttta tacatgagtg tatttctttt aatcattttt attgttggat
      181 gtcgaaatat gaaagatgaa cagaaaaaag aagaacaaac aaataaaaca gattcaaaag
      241 aagaacaaat caaaaagagt tttgagaaaa cattagatat gtatccaatt aagaatctcg
      301 aggagttata cgacaaagaa ggataccgag atggcgaatt taaaaagggt gataaaggga
      361 tgtggacgat atatacagat ttcgccaaaa gtaataaaca aggtggattg agtaatgaag
      421 gtatggtctt atacttagat agaaatacac ggactgcaaa gggacattat tttgttaaga
      481 cattctataa taagggcaaa ttccc
//
LOCUS       DALAGF010000242          482 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.252, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000242 DALAGF010000000
VERSION     DALAGF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.252"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..>482
                     /locus_tag="O2692_002837"
     CDS             <1..>482
                     /locus_tag="O2692_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCZ4300388.1"
                     /translation="MDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWAD
                     EEYREDLKAELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLATAIRKVETSTLGEES
                     ENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSGMEIDMLGDAYEFL
                     "
BASE COUNT          158 a           78 c          106 g          140 t
ORIGIN      
        1 atggatgcga gtgaattccg taattacatt ttaggcttga ttttctatcg cttcttatct
       61 gaaaaagccg aacaagaata tgcagatgcc ttgtcaggtg aagacatcac gtatcaagaa
      121 gcatgggcag atgaagaata tcgtgaagac ttaaaagcag aattaattga tcaagtcggt
      181 tacttcattg agccacaaga tttattcagt gcgatgattc gtgaaattga aacgcaagat
      241 ttcgatatag aacatctagc gacggcgatt cgcaaagttg aaacatctac attaggtgaa
      301 gaaagtgaaa atgactttat cgggctgttc agcgatatgg atttgagttc aacgcgacta
      361 ggtaacaatg tcaaagaacg tactgcttta atctctaaag tcatggttaa tcttgacgac
      421 ttaccattcg ttcacagtgg catggaaatt gatatgttag gtgatgcata tgaattccta
      481 at
//
LOCUS       DALAGF010000243          482 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.253, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000243 DALAGF010000000
VERSION     DALAGF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.253"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..>482
                     /locus_tag="O2692_002838"
     CDS             <1..>482
                     /locus_tag="O2692_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCZ4300389.1"
                     /translation="MDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWAD
                     EEYREDLKAELIDQVGYFIEPEDLFSAMIREIETQDFDIEHLATAIRKVETSTLGEES
                     ENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEIDMLGDAYEFL
                     "
BASE COUNT          159 a           80 c          106 g          137 t
ORIGIN      
        1 atggatgcga gtgaattccg taattacatt ttaggcttga ttttctatcg cttcttatct
       61 gaaaaagcgg aacaagaata tgcagatgcc ttgtcaggtg aagacatcac gtatcaagaa
      121 gcatgggcag acgaagaata ccgtgaagac ttaaaagcag aattaattga ccaagtcggt
      181 tacttcattg agccagaaga tttattcagt gcgatgattc gtgaaattga aacgcaagat
      241 ttcgatatcg aacacctggc gacggcaatt cgtaaagttg aaacatcaac attaggtgaa
      301 gaaagtgaaa atgactttat cggtctgttc agcgatatgg atttgagttc aacgcgacta
      361 ggtaacaatg tcaaagaacg tactgcttta atctctaaag tcatggttaa tcttgacgac
      421 ttaccattcg ttcacagtga catggaaatt gatatgttag gtgatgcata tgaattccta
      481 at
//
LOCUS       DALAGF010000244          473 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.254, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000244 DALAGF010000000
VERSION     DALAGF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.254"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..>473
                     /gene="fmhC"
                     /locus_tag="O2692_002839"
     CDS             <1..>473
                     /gene="fmhC"
                     /locus_tag="O2692_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HCZ4300390.1"
                     /translation="FILVDPYLIENLRDANGRIIKNYNNSVIVKMLGKIGYLHQGYTT
                     GYSNKSQIRWISVLDLKDKDENQLLKEMEYQTRRNIKKTIEIGVKVEDLSIEETNRFY
                     KLFQMAEEKHGFHFMNEDYFKRMQEIYKDKAMLKIACINLNEYQDKLKIQLLKIE"
BASE COUNT          208 a           37 c           81 g          147 t
ORIGIN      
        1 tttattcttg ttgatccata tttaatagag aatttaagag atgcaaatgg taggataata
       61 aagaattata ataattcagt gatagtaaag atgctaggga aaattgggta tctccatcaa
      121 ggttatacaa caggatattc aaataaaagt caaattaggt ggatttctgt attggattta
      181 aaagataaag atgagaatca acttttaaaa gaaatggaat accaaactag aagaaatata
      241 aaaaagacta ttgagattgg tgttaaggtt gaagatttat ctattgaaga aacaaatcga
      301 ttttataaat tgtttcaaat ggctgaagaa aaacatggtt ttcatttcat gaatgaagat
      361 tattttaaac gaatgcaaga aatatataaa gataaggcaa tgttaaagat agcttgtata
      421 aatcttaatg aatatcaaga taaattaaaa atacaattat tgaaaatcga aaa
//
LOCUS       DALAGF010000245          469 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.255, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000245 DALAGF010000000
VERSION     DALAGF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.255"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            53..303
                     /locus_tag="O2692_002840"
                     /pseudo
     CDS             53..303
                     /locus_tag="O2692_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187510.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
BASE COUNT          183 a           61 c           67 g          158 t
ORIGIN      
        1 agttaatcaa ttttgcatct attttgttgt aagctatata aaaggagtga taatgatggt
       61 gaaaaaaaca aaatccaatt cactaaaaaa agttgcaaca cttgcattag caaatttatt
      121 attagttggt gcacttactg acaatagtgc caaagccgaa tctaagaaag atgatactga
      181 tttgaagtta gttagtcata acgtttatat gttatcgacc gttttgtatc caaactggag
      241 acttttaaca taaaattact tatcattcaa aaagtaaaac agcataatat caaggtttat
      301 aactttatca ttatcaataa tacctcatat aaaataaaat tttagggact ttttagggac
      361 tttaaattta aaattacaag tttaatagaa acatcaaaat aatcacatgt ttgtgtggaa
      421 tgtacacccc aaaagctaga ctgaaaaatc tattttttga ggtgtattt
//
LOCUS       DALAGF010000246          447 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.256, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000246 DALAGF010000000
VERSION     DALAGF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.256"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            50..>447
                     /gene="lpl10"
                     /locus_tag="O2692_002841"
     CDS             50..>447
                     /gene="lpl10"
                     /locus_tag="O2692_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl10"
                     /protein_id="HCZ4300391.1"
                     /translation="MSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEEQIKKSFAKTL
                     DMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTILTGFSKSNKPGVLDDEGMVLYLNRNA
                     KKATGYYFVNKVYDDISKNHNEKKYRVELK"
BASE COUNT          196 a           40 c           92 g          119 t
ORIGIN      
        1 aaacagagag atgtgaaatg atggagtata taaaaaaatt gctttgtaca tgagtgtatt
       61 acttttaatc atttttattg ggggatgtgg aaatatgaaa gatgaacaga aaaaagagga
      121 acaaacgaat aaaacagatt caaaagaaga acaaatcaaa aagagttttg cgaaaacgtt
      181 agatatgtat ccaattaaga atctcgagga tttatacgac aaagaaggat atcgagatgg
      241 tgagtttaaa aaaggcgata aaggtacgtg gactatactt acaggttttt caaaaagtaa
      301 caaaccagga gtattagatg atgaaggcat ggtgttatat cttaatagaa atgccaaaaa
      361 ggcaacaggt tattattttg taaataaagt ttatgatgat attagcaaaa atcataatga
      421 gaaaaaatat cgtgttgaac taaaaaa
//
LOCUS       DALAGF010000247          437 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.257, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000247 DALAGF010000000
VERSION     DALAGF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.257"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            3..209
                     /locus_tag="O2692_002842"
     CDS             3..209
                     /locus_tag="O2692_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300392.1"
                     /translation="MNEIETIISEIEKLLTNNTPYSISKNSGVPRQTVTDLKVGKTKI
                     KEAKFKTIIKLYEYQRTLENKTEC"
BASE COUNT          188 a           50 c           62 g          137 t
ORIGIN      
        1 taatgaatga gattgaaact attataagtg aaatagaaaa gttattaact aacaatacac
       61 catatagtat ttcaaaaaac tcaggtgtac cacgtcaaac agttactgat ttaaaggtag
      121 gtaaaactaa aataaaagaa gctaaattta aaacgataat caagttatat gaatatcaaa
      181 gaacattaga aaataaaaca gaatgttaac aaaaatattt tgttaggtta ggtggacaag
      241 aaattaaatt tctaatgatt tcatggttgt ctgtccacgt tttgtccacc tcaataagat
      301 aatctcttag ttttagaaat tgtacatggt taaataatca aaaatgcata ttatgaaatt
      361 atcattgcca ttaaatcttg aatgatcttt aagcgatgat ataaaaggct gaacacagta
      421 aataagttaa ctttaat
//
LOCUS       DALAGF010000248          428 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.258, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000248 DALAGF010000000
VERSION     DALAGF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.258"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(88..321)
                     /locus_tag="O2692_002843"
     CDS             complement(88..321)
                     /locus_tag="O2692_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300393.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
BASE COUNT          146 a           71 c           43 g          168 t
ORIGIN      
        1 ataattagat tatctaattt tcatttgctt tacatgtaaa aggctatata tagtatgctc
       61 tttatgattc taaatgcttt ttaatattta atgctcatca acatttggat tttgaatatt
      121 caattcaaaa actttattag ctacgtcaat tgtaaaatca gaaccatagt tgacatgagc
      181 tacttttaat tttccatcta aataatagat tgcgattgca acatcgtaaa attcgtcaat
      241 gacaaataaa ctcttttcgt ttgttacaac ctcatgctct cctgagtata caacgttaat
      301 ttcccaatca ttaaaaacca tttgttaacc tccttgaaca tttaaattga ttcaacttaa
      361 gtttaactta ttcatacaac ttcgtacaat atctagatga acattaattg tatttctaga
      421 aatctttt
//
LOCUS       DALAGF010000249          401 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.259, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000249 DALAGF010000000
VERSION     DALAGF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.259"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..>401
                     /gene="tnpA"
                     /locus_tag="O2692_002844"
     CDS             <1..>401
                     /gene="tnpA"
                     /locus_tag="O2692_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCZ4300394.1"
                     /translation="KWNCKYHIVFAPKYRRQVIYGKIKKDIGIILRQLCERKGVEIIE
                     AEACKDHIHMLVSIPPKLGVSSFVGYLKGKSSLMIFDRHANLKYRYGNRKFWCKGFYV
                     DTVGRNKKVIENYIRNQLQEDIVADQISMEE"
BASE COUNT          164 a           36 c           82 g          119 t
ORIGIN      
        1 aaatggaatt gtaagtatca tattgtcttt gcacctaaat acagaagaca agtgatatat
       61 ggaaaaataa aaaaagatat agggattata ttgcgtcaat tatgtgaaag aaaaggtgta
      121 gagataattg aagcagaggc atgtaaagat catatccata tgttagttag tataccaccc
      181 aaacttgggg tatcatcatt tgttggctat ttaaaaggaa aaagtagttt aatgatattt
      241 gatagacatg ctaacttaaa gtatagatat ggaaatagaa agttttggtg taaaggattt
      301 tatgtggata cagtaggtag aaataaaaaa gtgattgaaa attatattcg taatcaatta
      361 caagaggata tcgttgcaga ccaaatctca atggaagaat a
//
LOCUS       DALAGF010000250          384 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.260, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000250 DALAGF010000000
VERSION     DALAGF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.260"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(86..301)
                     /locus_tag="O2692_002845"
     CDS             complement(86..301)
                     /locus_tag="O2692_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300395.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
BASE COUNT          121 a           58 c           45 g          160 t
ORIGIN      
        1 tgcttttcta tccaataaaa aagaagagaa gatgtaacac atcttctctt ctgcaatatt
       61 aattaggatt tatttctaag ttgagttatt ttaattgtaa atctgttttc tttaattctt
      121 ttataacttc tgcagtatca taacaatttg ttgcaattgt tgaatatctc tctgctaaac
      181 gatatgcatt aatgtaaagc tttaaacttt ctttagctat atcctctgca tcttcgaatt
      241 ttgatgggtt agacataacc actaattctg caaatttttc tggatcaata ttaatagaca
      301 tgtatttatt tacaactcct atttattttg atgtcttaat actaacatat tgaagttttc
      361 agacaaagta atgtctctct ataa
//
LOCUS       DALAGF010000251          376 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.261, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000251 DALAGF010000000
VERSION     DALAGF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.261"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..100
                     /locus_tag="O2692_002846"
     CDS             <1..100
                     /locus_tag="O2692_002846"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCZ4300396.1"
                     /translation="ENSGSNNATLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT          163 a           49 c           48 g          116 t
ORIGIN      
        1 tgaaaatagc ggctcaaata acgcaacgtt atttggcgga ttattcgcag cattaggatc
       61 attattgtta ttcggtcgtc gtaaaaaaca aaataaataa tacagcttat accaggtccg
      121 tgaggcctgg ttttttattt caaaatttat gaaatgtatc ttatgtttga gattaaatag
      181 tgcaattttt taaaaaatat aacataaatc aacataaatt taattcatct ttacattaaa
      241 agtacaaacg aataagaaaa gcaattaaac acagtatcaa accatttaaa acaattgata
      301 taaaaaacaa acgaataatt aaaaagtact agaattaatt tattcaaacc aaagtgtaaa
      361 ataacagtct tagata
//
LOCUS       DALAGF010000252          372 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.262, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000252 DALAGF010000000
VERSION     DALAGF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.262"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(24..>372)
                     /locus_tag="O2692_002847"
     rRNA            complement(24..>372)
                     /locus_tag="O2692_002847"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           86 a           95 c           88 g          103 t
ORIGIN      
        1 agatgttccg aatatatcct tagaaaggag gtgatccagc cgcaccttcc gatacggcta
       61 ccttgttacg acttcacccc aatcatttgt cccaccttcg acggctagct cctaaaaggt
      121 tactccaccg gcttcgggtg ttacaaactc tcgtggtgtg acgggcggtg tgtacaagac
      181 ccgggaacgt attcaccgta gcatgctgat ctacgattac tagcgattcc agcttcatgt
      241 agtcgagttg cagactacaa tccgaactga gaacaacttt atgggatttg cttgacctcg
      301 cggtttcgct gccctttgta ttgtccattg tagcacgtgt gtagcccaaa tcataagggg
      361 catgatgatt tg
//
LOCUS       DALAGF010000253          371 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.263, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000253 DALAGF010000000
VERSION     DALAGF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.263"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            <1..213
                     /locus_tag="O2692_002848"
     CDS             <1..213
                     /locus_tag="O2692_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ4300397.1"
                     /translation="WTSCEFDFTRDGKLNVSFDYIDWVNSEFGPMGREHYYMYKKFGI
                     WPEKEYAINWVEKIKDYVKEQDEAEL"
     gene            224..>371
                     /locus_tag="O2692_002849"
     CDS             224..>371
                     /locus_tag="O2692_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HCZ4300398.1"
                     /translation="MTFEGKLSQMYNEIANEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKP"
BASE COUNT          143 a           38 c           84 g          106 t
ORIGIN      
        1 tggacatcat gcgaatttga ctttacaaga gatggcaaat tgaatgtatc ttttgattat
       61 attgattggg tgaattcaga atttggacca atgggaagag aacattatta tatgtataaa
      121 aaatttggaa tttggcctga aaaagaatat gccataaatt gggttgaaaa aataaaagat
      181 tatgttaaag agcaagatga agctgaacta taggggagat aatatgactt tcgaaggaaa
      241 actaagtcaa atgtacaacg agattgcgaa tgagattagt gggatgatac cggtagaatg
      301 ggagcaagta tttacaatag cttatgtaac tgatcaagct ggagaagtca tctttaatta
      361 tactaaacca g
//
LOCUS       DALAGF010000254          358 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.264, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000254 DALAGF010000000
VERSION     DALAGF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.264"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
BASE COUNT          128 a           59 c           38 g          133 t
ORIGIN      
        1 tcacaaataa tctataattg agtttaaaat taactgatta ttaactattg tccgttaaac
       61 gaagatgatt acttcgtata ctgttataac tgaaatgttc tatatatcat ttcgtaatat
      121 cgcttacttc atctaaaaaa atttaatgct atgatttagt ctaattaaaa acatatatat
      181 atttactatg aactaaaatt tcactttaaa aataaaaaac catgatttcg aagtcttcat
      241 aaactgaagc ttctactctc atggcaattt atactatgat attcaattat aatgcctagt
      301 ctcatagtaa acggctacta cattcgcatg tacttcgttt tactgtccac gcatggtc
//
LOCUS       DALAGF010000255          347 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.265, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000255 DALAGF010000000
VERSION     DALAGF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.265"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            40..>347
                     /locus_tag="O2692_002850"
     CDS             40..>347
                     /locus_tag="O2692_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCZ4300399.1"
                     /translation="MNFEEKLSEMYNKIANEINEMIPVEWEKVYVIAYVDDGGGQVIF
                     YYTKPRNDELYYYSSIVEDYNVLEEIFDDLWMELYRSFKKLRNIFKEESLEPWTSCEF
                     "
BASE COUNT          141 a           30 c           74 g          102 t
ORIGIN      
        1 ttaaagagca agaagaagct gaacaataga ggcgataaca tgaatttcga agaaaaatta
       61 agtgaaatgt acaataaaat tgcaaatgag atcaatgaaa tgataccagt agagtgggaa
      121 aaggtatatg taattgcata tgtggacgat ggaggtgggc aagttatatt ttattatacc
      181 aagcccagaa atgatgagct gtattattat tcgagtatcg ttgaagatta taatgtttta
      241 gaagaaatct ttgatgattt atggatggaa ctttatagat catttaaaaa attaagaaat
      301 atatttaaag aagaaagtct tgaaccatgg acatcatgtg aatttga
//
LOCUS       DALAGF010000256          326 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.266, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000256 DALAGF010000000
VERSION     DALAGF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.266"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            19..231
                     /locus_tag="O2692_002851"
     CDS             19..231
                     /locus_tag="O2692_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300400.1"
                     /translation="MNQFKEIYNTIEKLLNDKSISNYRINQDTDVSYGGISELRSGKR
                     KVNNLTLETAEKLYNYQKQLEIMIED"
BASE COUNT          149 a           29 c           45 g          103 t
ORIGIN      
        1 ttcattttaa caaaaattat gaaccagttt aaagaaattt ataatacaat agaaaaatta
       61 ctaaatgata aatcaatatc taattataga attaatcaag acactgatgt ttcttatggt
      121 ggtataagtg aattaagaag cgggaaaaga aaagtgaata atttaacttt agaaacagcg
      181 gaaaaactct ataattacca aaaacaatta gaaataatga ttgaagatta aaataaggcc
      241 atatttttga aatatcatgg agtgagcctt ttaagtcatc taattttaaa gtatattaaa
      301 tatggataac ttaatgtgaa tataaa
//
LOCUS       DALAGF010000257          313 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.267, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000257 DALAGF010000000
VERSION     DALAGF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.267"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(82..>313)
                     /locus_tag="O2692_002852"
     CDS             complement(82..>313)
                     /locus_tag="O2692_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300401.1"
                     /translation="NMKRDFFEKWSFILNLILFCAFLFLVVLMFYKDVDLIYIFCTLL
                     ISLLPLLNIIKRIGWEINQSSMIYEVILAVID"
BASE COUNT          126 a           52 c           33 g          102 t
ORIGIN      
        1 ctgtagaatt tcttttcgaa attctctgtg ttggggcccc tgactagaat tgaaaaaagc
       61 ttattacaag cgcattttcg ttcagtcaat tactgccaat ataacttcgt agatcataga
      121 agattgattt atttcccagc ctattctttt tataatattt agcaaaggta ataatgatat
      181 taataatgta caaaaaatat aaatcaaatc gacatcctta taaaacatca gaaccactaa
      241 aaacaaaaaa gcacaaaata aaattaaatt taaaataaac gaccactttt caaaaaaatc
      301 tcttttcata ttt
//
LOCUS       DALAGF010000258          312 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.268, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000258 DALAGF010000000
VERSION     DALAGF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.268"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            56..160
                     /locus_tag="O2692_002853"
     CDS             56..160
                     /locus_tag="O2692_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300402.1"
                     /translation="MYFKNSSLNYIPTTFGGEPKKKHFPKMESASSEP"
     gene            complement(151..223)
                     /locus_tag="O2692_002854"
     tRNA            complement(151..223)
                     /locus_tag="O2692_002854"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(188..190),aa:Lys,seq:ttt)
     gene            complement(227..301)
                     /locus_tag="O2692_002855"
     tRNA            complement(227..301)
                     /locus_tag="O2692_002855"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(267..269),aa:Gln,seq:ttg)
BASE COUNT          107 a           67 c           63 g           75 t
ORIGIN      
        1 gaagacaacc aagcccaata atggactggc cgcctaataa taaaagctct aaaagttgta
       61 ttttaaaaat agttctttaa attatatacc caccacattt ggtggagaac ctaaaaaaaa
      121 gcacttccca aaaatggaaa gtgcaagtag tgagccatag aggattcgaa cctctgaccc
      181 tctgattaaa agtcagatgc tctaccaact gagctaatgg ctctaatggc tgggctagct
      241 ggattcgaac caacgagtga cggagtcaaa gtccgttgcc ttaccgcttg gctatagccc
      301 aatatataga tg
//
LOCUS       DALAGF010000259          301 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.269, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000259 DALAGF010000000
VERSION     DALAGF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.269"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
BASE COUNT          119 a           35 c           50 g           97 t
ORIGIN      
        1 gtggaataat taataataat tgtatttaaa tgcaatgctg ttgttctttc atacttacaa
       61 gcaagtcata agaaatgaga aattaaattc ttatatgatg aataaatgat gactgcagaa
      121 gaaattgcta aaaaaggagt ggagtgaggc attctactgt tcatattgtt aacaaatatc
      181 aaaatgatag gaacttgaag aatctaagac tgttaaaatc gagacaataa gaaactcgtt
      241 ccaaatttga atcctataat gaattcgtct catgaagaag ttatttactt ttcattagac
      301 t
//
LOCUS       DALAGF010000260          283 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.270, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000260 DALAGF010000000
VERSION     DALAGF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.270"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(1..181)
                     /locus_tag="O2692_002856"
                     /pseudo
     CDS             complement(1..181)
                     /locus_tag="O2692_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           85 a           43 c           42 g          113 t
ORIGIN      
        1 aattcttcat gattttctta ttggatttcg aatttggttt catgcattga tgtctcaaag
       61 aacatgatga acagtcatca cattcatata gtttgaagtc tcgtttaaaa ccatatctat
      121 cattacggta tgcatatctt ttaaaaccta ttcttttgtt attaggacat ataaattcat
      181 cgaattcgct atcagatatc gtttcaacaa tttcattaac atatcgcgta ttatcatttt
      241 gaggaattct aacagaagtt tctattggta gtgtaagttg ggt
//
LOCUS       DALAGF010000261          279 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.271, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000261 DALAGF010000000
VERSION     DALAGF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.271"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            22..>279
                     /locus_tag="O2692_002857"
     CDS             22..>279
                     /locus_tag="O2692_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCZ4300403.1"
                     /translation="MKHFAQLTLPIETAVRIPQNDISRYVNEIVKKIADSEFDEFRHH
                     RGATSYHLKMMLKITSYSYTQSEFSGRRIEKLLHNSIRMMWL"
BASE COUNT          107 a           48 c           38 g           86 t
ORIGIN      
        1 tactttgaaa tacttaaaaa aataaaacac tttgcccaac ttacactacc aatagaaact
       61 gctgttagaa ttcctcaaaa tgatatttcg cgatatgtta atgaaattgt taaaaagata
      121 gctgatagcg aattcgatga attcagacat catcgtggcg caacatccta tcatctaaaa
      181 atgatgttaa aaatcacctc atattcatat actcaatctg aattttctgg ccgtagaata
      241 gaaaaattac ttcataacag tattcgaatg atgtggtta
//
LOCUS       DALAGF010000262          277 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.272, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000262 DALAGF010000000
VERSION     DALAGF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.272"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            47..>277
                     /locus_tag="O2692_002858"
     CDS             47..>277
                     /locus_tag="O2692_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300404.1"
                     /translation="MEKGIFNYDNANVLKLDTNQLNENIKVIDDIFKNYEQIEPTIEV
                     ENGNTKLKLNGYFIASIISPLNLNKLNNLYVEE"
BASE COUNT          122 a           23 c           42 g           90 t
ORIGIN      
        1 gtataaaatt attttgtaaa acaattttgt tattaagagg tgcgctatgg aaaaggggat
       61 tttcaattac gataatgcga atgtattaaa attagatact aatcagttaa atgaaaacat
      121 aaaagtcatt gacgatatat ttaaaaatta tgagcagata gagccaacta tagaagtcga
      181 aaatggaaat acaaaattaa aattaaatgg atattttatt gcgtcaatta taagcccatt
      241 aaatttaaat aaacttaata atttatatgt tgaagaa
//
LOCUS       DALAGF010000263          275 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.273, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000263 DALAGF010000000
VERSION     DALAGF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.273"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            complement(<1..>275)
                     /locus_tag="O2692_002859"
     CDS             complement(<1..>275)
                     /locus_tag="O2692_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300405.1"
                     /translation="DFYKMKEEEFFLKSKLPLYLYSFNYVDEYTMKLLDRGRISILNT
                     KTNKDCIFDISKYIHENIIFRRDIFELNDIRDDDLKYKINGYNIEVY"
BASE COUNT           96 a           39 c           21 g          119 t
ORIGIN      
        1 agtatacctc aatattgtaa ccattaattt tatacttcaa atcatcatct cttatatcat
       61 ttaattcaaa tatatctctt ctgaatataa tattctcatg tatatacttt gaaatgtcaa
      121 atatacaatc tttatttgtt ttcgtattta atatagatat tcttcctcta tcaagtaatt
      181 tcattgtata ttcatctaca tagttgaagg aatataaata taaaggtagc ttagatttta
      241 agaaaaattc ttcttccttc attttataaa agtct
//
LOCUS       DALAGF010000264          274 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.denovo.274, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000264 DALAGF010000000
VERSION     DALAGF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.denovo.274"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
BASE COUNT          106 a           34 c           35 g           99 t
ORIGIN      
        1 aaaatgggta taaatagtag aagaagttaa cttggaagag ttaagctata acaaagaatc
       61 tctttagaca cacattgaat atcgaaacat ttaattgcgc taaatcgttt cattaaataa
      121 attaccttgt attgtcgatt aaattaaggt aaattataaa aaatgctgat atttttgact
      181 aaaccaaatg ctaacccaga aatacaatca ctgtgtctaa tgaataattt gttttataaa
      241 cacttttttg tttacttctc atttttaatt agtt
//
LOCUS       DALAGF010000265         5833 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.guided.135, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000265 DALAGF010000000
VERSION     DALAGF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.guided.135"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            458..1924
                     /gene="msr(A)"
                     /locus_tag="O2692_002860"
     CDS             458..1924
                     /gene="msr(A)"
                     /locus_tag="O2692_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCZ4300406.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
     gene            2023..2922
                     /locus_tag="O2692_002861"
     CDS             2023..2922
                     /locus_tag="O2692_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HCZ4300407.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            3408..4016
                     /locus_tag="O2692_002862"
     CDS             3408..4016
                     /locus_tag="O2692_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ4300408.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            4262..4600
                     /locus_tag="O2692_002863"
     CDS             4262..4600
                     /locus_tag="O2692_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300409.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            4605..4790
                     /locus_tag="O2692_002864"
     CDS             4605..4790
                     /locus_tag="O2692_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCZ4300410.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(5017..5760)
                     /locus_tag="O2692_002865"
     CDS             complement(5017..5760)
                     /locus_tag="O2692_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCZ4300411.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
BASE COUNT         2189 a          845 c          911 g         1888 t
ORIGIN      
        1 tgttaaaatt atattataaa ttcaactttg caacagaacc gtattttatg agtacactct
       61 aaattaagga gatgtataat caatctaaaa ttgtattttt atacttttgt tatataatct
      121 ttctacttag agatatattg taaaaaaacc aacaaaatgt tatattaaca ttactatttt
      181 atcgatgaag gaggattaaa atgacagctt ctatgagact caaataaacc tttttgaata
      241 caaaactaaa ttaattcaaa aaggcttgtc cgcaatacac actcatagga tgacttatgt
      301 cattttttat ccttcgatag gaataataaa cgctttcaaa tactaaaaca tcataataaa
      361 tttttaagtg tttgaaagtg taatgtgaat gagacgggag tagcctgtct catttttttg
      421 cgtacacgtc ctttaaatat acaaggagtg tataaatatg gaacaatata caattaaatt
      481 taaccaaatc aatcataaat tgacagattt acgatcactt aacatcggtc atctttacgc
      541 ttaccaattt gaaaaaatag cacttattgg gggtaatggt actggcaaaa ccacattact
      601 aaatatgatt gctcaaaaaa caaaaccaga atctggaaca gttgaaacgg ttggcgaaat
      661 tcaatatttt gaacagctta acatggatgt ggaaaatgat tttaacacgt tagacggtag
      721 tttaatgagt gaactccata tacctatgca tacaaccgac agtatgagtg gtggtgaaaa
      781 agcaaaatat aaattagcta atgtcatatc aaattatagt ccgatattac ttttagatga
      841 acctacaaat cacttggata aaattggtaa agattatctg aaaaatattt taaaatatta
      901 ctatggtact ttaattatag taagtcatga tagagcactt atagaccaaa ttgctgacac
      961 aatttgggat atacaagaag atggcacaat aagagtgttt aaaggtaatt acacacagta
     1021 tcaaaatcaa tatgaacaag aacagttaga acaacaacgt caatatgaac agtatataag
     1081 tgaaaaacaa agattgtccc aagccagtaa agctaaacga aatcaagcgc aacaaatggc
     1141 acaagcatca tcaaaacaaa aaaacaaaag tatagcacca gatcgtttaa gtgcatcaaa
     1201 acaaaaaggc acggttgaga aggctgctca aaaacaagct aagcatattg aaaaaagaat
     1261 ggaacatttg gaagaagttg aaaaaccaca aagttatcat gaattcaatt tcccacaaaa
     1321 taaaatttat gatatccata ataattatcc aatcattgca caaaatctaa cattggttaa
     1381 aggaagtcaa aaactgctaa cacaagtacg attccaaata ccatatggca aaaatatagc
     1441 gctcgtaggt gcaaatggtg taggtaagac aactttactt gaagctattt accaccaaat
     1501 agagggaatt gattgttctc ctaaagtgca aatggcatac tatcgtcaac ttgcttatga
     1561 agacatgcgt gacgtttcat tattgcaata tttaatggat gaaacggatt catcagaatc
     1621 attcagtaga gctattttaa ataacttggg tttaaatgaa gcacttgatc gttcttgtaa
     1681 tgttttgagt ggtggggaaa gaacgaaatt atcgttagca gtattatttt caacgaaagc
     1741 gaatatgtta attttggatg aaccaactaa ttttttagat attaaaacat tagaagcatt
     1801 agaaatgttt atgaataaat atcctggaat cattttgttt acatcacatg atacaaggtt
     1861 tgttaaacat gtatcagata aaaaatggga attaacagga caatctcttc atgatataac
     1921 ttaaaaacta tacactaatt tatattgaat attaggttca tttatatcaa tataaaaaat
     1981 acgttcacaa gaaaaatata atagaaataa ggatccaata aaatgactcg acataatgaa
     2041 attattaaat gtgcagaaaa atatcaatta cacatccaac ctcaaacaat ctcattgaat
     2101 gaatcgggac ttgattttca agttgcattt ggaaaagata aacatggagt agaatgggtt
     2161 ttgagactac caagaagacc tgacgtttat aaacgaacaa aacccgaaaa acaaacggta
     2221 gacttcttac agaagaatgt ttcatttgaa ataccgaagt ggaaagtaca tgcaaaagac
     2281 cttattgctt acccaaaact tacaggtaaa cccgcagcca caatagatcc agaaatacaa
     2341 aattatgtat gggaaattga acacaaacca ttaccagaaa actttattaa cacattagct
     2401 gaaacactcg tagatttaca caacatacca gaagaaaaca ttaacgttca gcatataaat
     2461 atcaaaacca tacaagaaat aaaaaatgac tttcaaagaa gaatgaataa agttaaagaa
     2521 acttatggcg tatcagatga attatggaac agatggaaac aatggttaga aaacgacgaa
     2581 ctatggcctc gacatgcgac catgatacat ggggacttac atccaggaca tataatggta
     2641 gataaccaag caaacgtcac aggtctcata gactggactg aagcaaccca ctccgaccca
     2701 tcaatggact ttataggaca ccatcgtgta ttcgacgacg aaggattaga gcaacttata
     2761 acagcatacg gtaaagctgg aggtgaaata tggccacgaa tgaaagagca tataatagaa
     2821 ctcaatgcag tattcccaat gtttatcgct gagtttgcta tggaatcagg agaatcggcg
     2881 tatgaaacga tggcattgaa agagttaggt atgaaagagt agaacttata ttaaaagagg
     2941 cccattttaa agacgaaggc aatcctgatg aaattttaaa actgcgttta attagtgatg
     3001 gtctttggtt ttctataatg tattaaatat ctggacatgg aacaaatgca acaaatggaa
     3061 agaatcgttt tcgaaatttc taactcctta aatcaggagg ataaataaac ggtcattttc
     3121 attgaaagca cttatataaa tttcatcaat tttaatacct accgtaaata catttcataa
     3181 gtattaaaca gatgattaaa atttttagtt aatttaaata tttatgatag agatgacctt
     3241 caaatgaacg gttctgttac aaagaaaaaa tctatattta tttttagtaa gttttaatta
     3301 tagatatgta cactttgagt acaccctaac catacaaaaa gaattgttga aattataaca
     3361 attaaaacgt atgattaggg tgtatattta ttatggagtg aagatgaatg attataggtt
     3421 atgcgagagt atcatcgata gatcaaaatt tagaaagaca attggataat ttaaagacgt
     3481 ttggtgtgga gaaaatcttt acagagaaac agtcgggaaa gtcagtggaa aatagacctg
     3541 tatttcaaga agcactcaat tttgtcagaa tgggagatcg attcgttgtg gaatctattg
     3601 atcgtttagg acgtaactat gatgaaatta ttgaaactgt gaattattta aaagaaaaag
     3661 acgttcagtt aatgattacc agcttaccta tgatgaatga agtaattggc aatccattac
     3721 tggataagtt tatgaaagat ttaatcattc aaatattagc aatggtttca gaacaagaga
     3781 gaaatgaaag taaacgtcga caagcgcaag ggattcaagt tgcgaaggaa aaaggcgtat
     3841 ataaagggcg ccctttactt tattcaccaa atgctaaaga cccacagaaa cgtgttattt
     3901 atcatcgcgt ggtggaaatg ttagaagaag gcaaagcgat tagtaagatt gcgaaagaag
     3961 ttaatatcac aagacaaaca gtttatagaa ttaaacatga taagggatta tcttgataaa
     4021 ttgattgtca atgcaaatga gcgtctctca cagtgtctca aattccataa catattattg
     4081 aattgagatt ttaataatta tacaagaaaa aagagtacga gttcgcagct ttctatatag
     4141 taattgtttt actaataagg tgttttaaaa tatattataa tgtaaaaagt atttgacatt
     4201 tgtatcaaaa atatttacac tcataaatgt caaatacttt ttacatttat gaggtgttat
     4261 aatgaattta attcctttta ttattatttt aattttttct tgtttatgga cggctataac
     4321 taacaaatat ttaccttcag aaacattaga taaaaaagga caacaagatc gatacgatga
     4381 aagacaacgt aaaatgttta ttgaaatact ggctaaaagt tttatttgga ttgtatattg
     4441 tatgttattt acactagtgc ttaaatttac aggtttgtca ccaagtattg aaggatcttg
     4501 gttttctcaa tatccagaaa ttttctttat aattgtagca ctatttctta tgctgtttaa
     4561 ttactatact acaaataaaa aatatacatc taaaggttaa attaatgaaa aatagaatta
     4621 gagaatatag aaaacaaaaa ggaatttctc aagaaacttt aagtaaagaa ttaaaagtgt
     4681 ctaggcaaac tattaatgca atagaaaaca ataaatatga tcctacattg acattagcat
     4741 ttaagttggc aagattgttt gatacaactg tggatgaatt attttcttaa ccattcattt
     4801 atcacaaaaa gagagtgcga attcgcactc tctttttgtg tagtagtact attaaatttt
     4861 gtctcaataa cgaacgttta taacgcaatc atgatgagag tattaataca gttaattatt
     4921 tgaaatactt tagtttacat aatcgatgat taccagaagt tctcactata taaaagacac
     4981 taaaatttta taacttttag tgcctaatca attattttag ctaccaaaca aacgactcca
     5041 aaaccccttt ttaggttccg tttcttcact cttttcttct atatcactat cattgtcatt
     5101 tagattagct ggaacgtctt ctttttttgc ttcttcacct tgtgtacttt cgtcgctttg
     5161 acttccagat atatccttat attgaatctc tgtttcttta gtttcttcct gatctctgtt
     5221 aatatttaca ggattagtag tatatgtttt ttcttgtgca tcaacgtttt gtctagtgtt
     5281 agtagatgta tcaaagggat aattaaggtg tttttctttt tctaattgat tttctaattt
     5341 ttgaatcttt ttattactct ctaatgccag aacttgctga ttttcaagca attttgatct
     5401 ttcttgcaac tcttttctgt aaaattcaat tgttgtttca tgacgacttt cttgtttttc
     5461 aatttgatta tttaaagtat caatctgatt tttaaggatt tctactattt gagcatcgct
     5521 tttcgattca aaattaatat tatccgaccc ttttttttgt ttgtcttcag tagtactttc
     5581 aaaaccatat ttctttttat tttcatttac tctttgaatg attttttcta tatcggtatc
     5641 ttctttaata aacgaagtgt tttcctgttt tattgtctct atattcaatc gcttaatatt
     5701 attaaaaatc gtttgcttac taacccctaa ttcttcactc aactttttca cacttttcat
     5761 atcatcaacc ctttataaaa tgtttataaa cccttgataa aatattataa gcttataatg
     5821 cttaataata aag
//
LOCUS       DALAGF010000266         4424 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.guided.149, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000266 DALAGF010000000
VERSION     DALAGF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.guided.149"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            111..1067
                     /locus_tag="O2692_002866"
     CDS             111..1067
                     /locus_tag="O2692_002866"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCZ4300412.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1267..1746
                     /locus_tag="O2692_002867"
     CDS             1267..1746
                     /locus_tag="O2692_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCZ4300413.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1761..2600
                     /gene="fakB2"
                     /locus_tag="O2692_002868"
     CDS             1761..2600
                     /gene="fakB2"
                     /locus_tag="O2692_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCZ4300414.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2686..3219
                     /gene="msrA"
                     /locus_tag="O2692_002869"
     CDS             2686..3219
                     /gene="msrA"
                     /locus_tag="O2692_002869"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCZ4300415.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            3212..3640
                     /gene="msrB"
                     /locus_tag="O2692_002870"
     CDS             3212..3640
                     /gene="msrB"
                     /locus_tag="O2692_002870"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCZ4300416.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3652..4152
                     /locus_tag="O2692_002871"
     CDS             3652..4152
                     /locus_tag="O2692_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCZ4300417.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            4152..4373
                     /locus_tag="O2692_002872"
     CDS             4152..4373
                     /locus_tag="O2692_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCZ4300418.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
BASE COUNT         1616 a          648 c          798 g         1362 t
ORIGIN      
        1 atattaatat catattccta aaatgagtga taacaaaatg ctacataaag ttaagttata
       61 tcaaactaaa tatacatact ataaataatg aaaatgaggt gttatcgcat atgttgaatt
      121 catttgatgc agcatatcac agtctttgtg aagaagtttt agaaatagga aatacacgaa
      181 atgatcgcac aaatacaggt acgatttcga aatttggtca tcaacttcgc tttgacttat
      241 ctaaaggatt tccactatta acgacaaaga aagtttcttt taaattagta gcaaccgaat
      301 tattatggtt cattaaagga gatacaaaca tccaatactt attaaaatat aataataata
      361 tatggaacga atgggctttt gaaaattata tcaaatcaga cgagtataaa ggtccagata
      421 tgacagattt cgggcatcgt gcattgagtg atcctgaatt taacgaacaa tataaagaac
      481 aaatgaaaca atttaagcaa cgtattcttg aagatgatac atttgcgaag caattcgggg
      541 atttaggaaa tgtttatggt aaacaatggc gagattgggt tgataaagat ggtaatcatt
      601 ttgatcaact taaaacagta attgaacaaa ttaagcataa tccagattca aggcgacaca
      661 tcgtatctgc atggaatcca acagaaattg atacaatggc acttccgcct tgtcatacca
      721 tgttccagtt ttatgtccaa gatggtaagt taagttgcca gttataccaa cgtagcgcag
      781 atatcttttt aggtgtgcca tttaatatcg caagctacgc tttattgaca caccttattg
      841 ccaaagaatg tggacttgaa gtgggtgaat ttgtgcatac atttggagat gcacatattt
      901 attcaaatca tattgatgcg attcaaacac aattagcacg tgaaagcttc aatcctccaa
      961 cattaaaaat taacagtgac aagtctattt tcgacataaa ttatgaagat ttggaaattg
     1021 ttgactatga atcacatcca gcaataaaag ctccaatagc agtgtagtca ttgcatagtt
     1081 agctaaccat atagacatca aaatgacatc atagtatttt caagtgcaaa aaagtacttt
     1141 tttgtgttaa acgttttcat aaattatgca aaatcattat ttctatcaca ctttatgata
     1201 aaaattgtgt taaattaaag ataacttagt aataaaaaat gaaatgatag aagaaggagg
     1261 ataattatga ctttatccat tctagttgca catgacttgc aacgagtaat tggttttgaa
     1321 aatcaattac cttggcacct accaaatgat ttgaagcatg ttaaaaaatt atcaacaggt
     1381 catactttag taatgggtcg taagacattt gaatcgattg gtaaaccact accgaatcgt
     1441 cgaaatgttg tacttacttc agatacaagt ttcaacgtag agggcgttga tgtaattcac
     1501 tctattgaag atatttacca actaccgggc catgttttca tatttggagg gcaaacatta
     1561 tttgaagaaa tgattgataa agtggacgac atgtatatta ctgttattga aggtaaattc
     1621 cgtggtgata cgttctttcc accttataca tttgaagact gggaagttgc ctcttcagtt
     1681 gaaggtaaac tagatgagaa aaatacaatt ccacatacct ttctacattt aattcgtaaa
     1741 aaataagggg gaaaacgacc atgacaaaac agattatagt aacagactca acatccgatt
     1801 tatctaaaga atacttagaa gcaaacaaca ttcatgtaat tcctttaagt ttaactattg
     1861 aaggagcttc atacgttgac caagtagata ttacatcaga agaatttatt aatcatattg
     1921 aaaatgatga agatgtaaag acaagtcagc cagccatagg tgaatttata tctgcttatg
     1981 aagaactagg aaaagatggc tctgaaatca taagtattca tctttcttca ggattaagtg
     2041 gtacatataa cactgcttac caagcaagtc aaatggtaga tgctaatgta actgttattg
     2101 attcaaaatc tatttctttt ggtttagggt atcaaataca acacctagta gagcttgtaa
     2161 aagaaggtgt ctcaacttct gaaatagtta aaaagttaaa tcatttaaga gaaaacatta
     2221 aattatttgt agttataggg caattgaatc aattaattaa aggtggcaga attagtaaaa
     2281 caaaaggttt gattggtaat cttatgaaaa ttaaaccaat tggtacacta gatgatggtc
     2341 gcttagagct tgtgcacaat gcgagaactc aaaattctag tatccaatac ttgaaaaagg
     2401 aaattgctga atttatagga gatcatgaaa tcaaatccat tggtgtcgca catgctaacg
     2461 tcattgaata tgttgataaa ttgaagaaag tttttaatga agcttttcat gtgaataatt
     2521 acgatataaa tgtaactaca ccagttattt ctgcacatac tggtcaaggt gcgattggcc
     2581 tcgtagtcct taagaagtaa atttaatctt ttcagtgtta attacttcca tttcaatcct
     2641 ttatagacta aatttataat tagatagata gaggaggtaa ttcatatgac aaaagaatat
     2701 gcaacattag caggaggatg tttctggtgc atggttaaac catttacatc atatccaggc
     2761 atcaagtcag tcgtatctgg ttatagtggc ggtcatgttg acaacccaac ttatgaacag
     2821 gtatgtacga atcaaaccgg ccatgtcgaa gcagtacaaa ttacgtttga tccagaggtt
     2881 acttcctttg aaaatatatt agacatatat ttcaaaacat ttgacccaac tgatgatcaa
     2941 gggcaatttt tcgatagagg cgaaagctat caaccagtca ttttctatca tgatgaacat
     3001 cagaaaaagg ctgctgagtt taaaaagcaa caattaaatg aacaaggtat tttcaagaaa
     3061 ccagtgatta cacctattaa accatataaa aatttctatc cagctgaaga ctaccatcaa
     3121 gattattaca aaaagaaccc ggtacattat taccaatatc aacgtggttc aggtagaaaa
     3181 gcgtttatag aatcacattg ggggaatcaa aatgcttaaa aaagataaaa gtgaactaac
     3241 agatatagaa tatattgtta cacaagaaaa cggcactgaa ccaccattta tgaatgaata
     3301 ttggaatcat tttgctaaag gaatttatgt agataaaatt tctggtaaac ctttatttac
     3361 atctgaagaa aagtttcatt ctgaatgtgg atggcctagc ttttccaaag cgcttgatga
     3421 cgatgaaatt atagaattag tcgacaaatc atttggtatg ttgagaactg aagtgcgttc
     3481 agaagaatca aatagtcatt taggacatgt ctttaatgat ggacctaaag aaagtggcgg
     3541 cttaagatac tgtatcaatt ccgctgcaat tcaatttatt ccatatgaaa agttagaaga
     3601 attgggttat ggcgatttaa tatcacattt tgataagtag gagtggaaag tatgtttaaa
     3661 aaattattcg gaaaaggcaa agaagttcag aaagatatcg caatttatgc accattaact
     3721 ggagaattcg tgaaaattga agatattcca gatcctgtat tcgcacaaaa aatgatgggc
     3781 gaaggttttg gtattaatcc aactgaagga gaagttgtgt ctccaattgc aggacgtgtt
     3841 gacaatgtct ttccaactaa gcatgcgatt gggttaaaag cagataacgg attagaatta
     3901 ttagttcata tcggtttaga cacagttcaa ttagacggtg aaggatttga agtgttagtg
     3961 tctagtggtg acgaagttaa cgtcggagat ccattagtaa gattcaacct tgaatatatt
     4021 aataataacg ctaaatctgt aatttcacca attataatta ctaacactga tcaagcagct
     4081 tcaattaata tttatgatga aaatgctgtg attaaaggtg aaacaaaagt gattgatgtg
     4141 acaatgaact aatgaaaaac tattcgtttt atcaatttgt catgacagtt cgtggtcgac
     4201 acgacgataa aggtcgtcta gcagaagaga tatttgacga tcttgctttc ccaaaacacg
     4261 atgatgattt taacatactg tctgattata ttgagacaca tggtgatttc acattgccaa
     4321 tgtctgtatt tgatgattta tatgaagaat atacggaatg gctaaaattt taatataatt
     4381 tttaataata atagttagaa ccagggtgat gcaattcgtt atcc
//
LOCUS       DALAGF010000267         3999 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.guided.151, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000267 DALAGF010000000
VERSION     DALAGF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.guided.151"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            1..420
                     /locus_tag="O2692_002873"
     CDS             1..420
                     /locus_tag="O2692_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCZ4300419.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(676..1611)
                     /locus_tag="O2692_002874"
     CDS             complement(676..1611)
                     /locus_tag="O2692_002874"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCZ4300420.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            1901..3160
                     /gene="sdpC"
                     /locus_tag="O2692_002875"
     CDS             1901..3160
                     /gene="sdpC"
                     /locus_tag="O2692_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HCZ4300421.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            complement(3226..3912)
                     /locus_tag="O2692_002876"
     CDS             complement(3226..3912)
                     /locus_tag="O2692_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HCZ4300422.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
BASE COUNT         1355 a          736 c          562 g         1346 t
ORIGIN      
        1 atgttaaaat ctattaatca tatatgcttt tcagtcagaa atttaaacga ttcaatacat
       61 ttttatagag atattttact tgggaaattg ctattgactg gtaaaaaaac tgcttatttt
      121 gagcttgcag gcctatggat tgctttaaat gaagaaaaag atataccacg taatgaaatt
      181 cacttttcat atacacatat agctttcact atagatgaca gcgaatttaa atattggcat
      241 cagaggttaa aagataataa cgtgaatatt ttagaaggaa gagttagaga tattagagat
      301 agacaatcaa tttactttac cgaccctgat ggtcataagc tagaattaca tactggcaca
      361 cttgagaaca gattaaatta ttataaagag gctaaaccac atatgacatt ttacaaataa
      421 ggtgtcatta taaaaaggcc tcttgaactc cgttaaaatt ttaattaatt attatataat
      481 aagagaactt ttcaaacaat acagttgtta tttttgctat ttcaacaaac ataaataagc
      541 agtaagatga ctacaactta agagtcttct tactgcaatt atttttcaaa tatatcaacg
      601 ttaatataac ttctattaag aaatactcac attctgccct gcaatgcaaa tctcgtcaca
      661 tataaatatt tttaattatt ttaaaaaatg atgcactaaa ttagcaacga gcttagcagt
      721 tctattgtca gcgtcatatg ttggattcat ctcagcaata ctaactgaag acaccttatc
      781 acttggaata atacgttttg ctaattcaag aacagtatgt ggatacaaac ctaacactgc
      841 cggcgcactt accccaggcg caaacgcact atcaatgaca tccatacaaa tcgtaaacat
      901 aatgacatca tgttcatgta caaaacgttc aatcatatct ttaattgttg gtgatacgtg
      961 actcaataat tcatctgcaa agacataatc aatctttttc tctttagcat aatcaaataa
     1021 actttgcgta ttaccacctt gagcaatacc aagcactaaa taatctgtgt tttcatcttc
     1081 ttctaaaatt tgtctaaagc tcgttccaga tgtagattgt tgttcagcac gtgtatcaaa
     1141 atgcgcatca atatttatca caccaataga ttgtgttgga tagactttac gtgttgctaa
     1201 atattgagca tacgcaatat catgtccacc acctaataaa aatgtttgtc tatgattagc
     1261 aattgacttc gctgcaagca tagcaaattc tttttgagta tcaattaatt cctcatgatc
     1321 atgataaaca tttccgtaat cgactaaagt ttcacattga ttcaaatccg gcaaacctgc
     1381 aaatgcttgt ttaatcgcat ctggtccttc ttttgcacca atgcgcccct tgtttaaagc
     1441 aacacctttg tcaacagcat agcctaatat accgacccct gatggcatac tactcttttc
     1501 cagcttagac aaatcttcaa atgttactgt ttgaaaatgt ctaaattttt tcgggtctgt
     1561 ttcactatct aaccttccag tccataaatt tggttcacct tgcttgtaca cagcatttcc
     1621 ccctcttatt tatgtggctt attaacaatt aaagtataac gtataggaaa ttttgaattc
     1681 aattcatagt taaatccgta tcttaaaaat acttatctac attactttta cccctatttt
     1741 ctatgtaata acgaatactt agctgattta tgttaataaa atacgtcaag actattacat
     1801 tttcattaat attgacatag acaatttatc tctcggcttg taatatgtat aattgttact
     1861 aaaagatatt ttgcttgtta cctaatggag gttacatata atgaagaaca ataaaatttc
     1921 tggttttcaa tgggcaatga cgattttcgt cttctttgtc attacaatgg cgttatccat
     1981 tatgctcaga gatttccagt ctataattgg tgtcaaacac tttatatttg aagttacaga
     2041 tctagcacca ttaattgctg caatcatttg tatactcgtt ttcaaatata aaaaggtcca
     2101 acttgcaggt ttaaaattct caatcagcct gaaagtaatt gaacgtctat tgctagcttt
     2161 aattttacct ttaattattc taattattgg tatgtacagc tttaatacat ttgcagatag
     2221 ctttatttta ttacaatcaa caggcttatc agtacctatt acacacattc tgattggaca
     2281 tattctgatg gcgttcgtag tagaattcgg attccgttca tacttacaaa atattgttga
     2341 aacgaaaatg aacacgttct ttgcaagtat tgttgttggt ttgatgtatt cagttttctc
     2401 agcaaataca acttatggta cagaatttgc tgcttataac ttcctttata cattctcatt
     2461 ctctatgatt cttggtgaat taattagagc gactaaagga cgtacaattt atattgcaac
     2521 gacattccat gcttcaatga cattcggact tattttcttg tttagcgaag aaatcggcga
     2581 tctattttca atcaaagtca tcgccatttc aacagcaatc gttgcagtag gatacattgg
     2641 tttaagctta attatccgag gtattgcata tttaacaaca agacgaaacc ttgaagaact
     2701 tgagcctaat aattatttag accatgtcaa tgacgatgaa gaaactaatc atactgaggc
     2761 tgaaaaatct tcttcaaata ttaaagatgc tgaaaaaaca ggtgtagcta ctgcatcaac
     2821 ggttggtgtt gctaaaaatg atactgaaaa tacagtggct gacgaaccaa gcattcatga
     2881 aggtactgaa aaaacagaac ctcaacatca cataggtaat caaactgaat ctaatcatga
     2941 tgaagatcat gacatcactt cggagtcagt agaatcagca gaatcagtta aacaagcacc
     3001 acaaagtgac gatttaacaa acgattcaaa tgaagatgaa atagagcaat cattaaaaga
     3061 acctgcgact tataaagaag acagacgttc atcagttgta attgatgcag aaaaacatat
     3121 cgaaaaaact gaagaacaat cttcagataa aaacaaataa taacaaagcg cttgctagta
     3181 cctcttaaaa agatgatgct agcaagcgct tttctatact atatattatt tttctaaaat
     3241 tttaacaccc tcttgagtgc ctacaataac ttgatctgcc atatctaaga agtatcctgt
     3301 ctcaaacaca cctgtcagat gaattaaata ctcatggaac ttatatgggt caattccttt
     3361 tggtaactta caatctaaaa tataattgcc attatcagtt ataaatgcga catcttcgtt
     3421 tacacgacgt tctactttta tatcagcgta tgattcaatt ttacgtaata tgtgatacca
     3481 gttaaattta tccacctcta ctggtaactt aaacgtctca cctaagtatt gaactatttt
     3541 cgtttcatcg acaaccacaa caaaacgcga tgccatttca tctataactt tctctctgaa
     3601 cagcgcacca ccgccacctt taattatatt taaagatgga tctacttcat cagcaccatc
     3661 aattgctaag tcgatatgat caacatcatt gatttcacat attttaatac ctaattcttt
     3721 tgctaaaaat gcaattttat tagaagtgca tacacctgta atattgtaac cacgttcttt
     3781 aattagttgc gccatttgag gtaagagtaa ttccattgta cttcctgtac caattcccag
     3841 cgtcatgtca ccattgattt gacttaaaac atcatttaat gtcattaact tgagtgcttt
     3901 gacatctttc atgaaggtag cctcccatat ttatgtaatc tattcaattc atattttaca
     3961 tgactcgtat aaattaacat acccttattt ctaacattt
//
LOCUS       DALAGF010000268         3817 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.guided.153, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000268 DALAGF010000000
VERSION     DALAGF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.guided.153"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            20..2029
                     /gene="mecA"
                     /locus_tag="O2692_002877"
     CDS             20..2029
                     /gene="mecA"
                     /locus_tag="O2692_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCZ4300423.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(2075..2503)
                     /locus_tag="O2692_002878"
     CDS             complement(2075..2503)
                     /locus_tag="O2692_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCZ4300424.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(2600..3343)
                     /locus_tag="O2692_002879"
     CDS             complement(2600..3343)
                     /locus_tag="O2692_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCZ4300425.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
BASE COUNT         1361 a          612 c          577 g         1267 t
ORIGIN      
        1 gtcttatata aggagtatat tgatgaaaaa gataaaaatt gttccactta ttttaatagt
       61 tgtagttgtc gggtttggta tatattttta tgcttcaaaa gataaagaaa ttaataatac
      121 tattgatgca attgaagata aaaatttcaa acaagtttat aaagatagca gttatatttc
      181 taaaagcgat aatggtgaag tagaaatgac tgaacgtccg ataaaaatat ataatagttt
      241 aggcgttaaa gatataaaca ttcaggatcg taaaataaaa aaagtatcta aaaataaaaa
      301 acgagtagat gctcaatata aaattaaaac aaactacggt aacattgatc gcaacgttca
      361 atttaatttt gttaaagaag atggtatgtg gaagttagat tgggatcata gcgtcattat
      421 tccaggaatg cagaaagacc aaagcataca tattgaaaat ttaaaatcag aacgtggtaa
      481 aattttagac cgaaacaatg tggaattggc caatacagga acagcatatg agataggcat
      541 cgttccaaag aatgtatcta aaaaagatta taaagcaatc gctaaagaac taagtatttc
      601 tgaagactat atcaaacaac aaatggatca aaattgggta caagatgata ccttcgttcc
      661 acttaaaacc gttaaaaaaa tggatgaata tttaagtgat ttcgcaaaaa aatttcatct
      721 tacaactaat gaaacagaaa gtcgtaacta tcctctagga aaagcgactt cacatctatt
      781 aggttatgtt ggtcccatta actctgaaga attaaaacaa aaagaatata aaggctataa
      841 agatgatgca gttattggta aaaagggact cgaaaaactt tacgataaaa agctccaaca
      901 tgaagatggc tatcgtgtca caatcgttga cgataatagc aatacaatcg cacatacatt
      961 aatagagaaa aagaaaaaag atggcaaaga tattcaacta actattgatg ctaaagttca
     1021 aaagagtatt tataacaaca tgaaaaatga ttatggctca ggtactgcta tccaccctca
     1081 aacaggtgaa ttattagcac ttgtaagcac accttcatat gacgtctatc catttatgta
     1141 tggcatgagt aacgaagaat ataataaatt aaccgaagat aaaaaagaac ctctgctcaa
     1201 caagttccag attacaactt caccaggttc aactcaaaaa atattaacag caatgattgg
     1261 gttaaataac aaaacattag acgataaaac aagttataaa atcgatggta aaggttggca
     1321 aaaagataaa tcttggggtg gttacaacgt tacaagatat gaagtggtaa atggtaatat
     1381 cgacttaaaa caagcaatag aatcatcaga taacattttc tttgctagag tagcactcga
     1441 attaggcagt aagaaatttg aaaaaggcat gaaaaaacta ggtgttggtg aagatatacc
     1501 aagtgattat ccattttata atgctcaaat ttcaaacaaa aatttagata atgaaatatt
     1561 attagctgat tcaggttacg gacaaggtga aatactgatt aacccagtac agatcctttc
     1621 aatctatagc gcattagaaa ataatggcaa tattaacgca cctcacttat taaaagacac
     1681 gaaaaacaaa gtttggaaga aaaatattat ttccaaagaa aatatcaatc tattaactga
     1741 tggtatgcaa caagtcgtaa ataaaacaca taaagaagat atttatagat cttatgcaaa
     1801 cttaattggc aaatccggta ctgcagaact caaaatgaaa caaggagaaa ctggcagaca
     1861 aattgggtgg tttatatcat atgataaaga taatccaaac atgatgatgg ctattaatgt
     1921 taaagatgta caagataaag gaatggctag ctacaatgcc aaaatctcag gtaaagtgta
     1981 tgatgagcta tatgagaacg gtaataaaaa atacgatata gatgaataac aaaacagtga
     2041 agcaatccgt aacgatggtt gcttcactgt tttattatga attattaata agtgctgtta
     2101 cttctccctt aaatacaatt tcttcatttt cattgtatgt tgaaagtgac actgtaacga
     2161 gtccattttc tttttttatg gatttcttat ttgtaatttc agcgataacg tacaatgtat
     2221 tacctgggta tacaggttta ataaatttaa cgttattcat ttgtgttcct gctacaactt
     2281 cttctccgta tttaccttct tctacccata atttaaatga tattgaaagt gtatgcatgc
     2341 cagatgcaat gataccttta aatctacttt gttctgcttt ttctttatct atatgcatat
     2401 attgaggatc aaaagttgtt gcaaattgga taatttcttc ttctgtaata tgaaggcttt
     2461 ttgttttgaa tgtttctcct actataaaat catcgtattt catatatgtc tctctttctt
     2521 attcaaatta attttttagt atgtaacatg ttaaaggtaa gtctaccgtc actgaaacgt
     2581 aagactcacc tctaactttc tattgagaca aatgcaccat tttatctgca ttgtctgtaa
     2641 agataccatc aactccccaa ttagcaagtt ggtttgcacg tgctggtttg tttacagtcc
     2701 atacgttcaa ttcataaccc gcttctttta ccatttttac ttttgcttta gtaagtttgg
     2761 catcttcagt gtttactatt ttagcattac agtaatctaa aagtgttctc cagtcttcac
     2821 gaaacgaagt tgtatggaat ataactgctc tgttatattg tggcatgatt tcttctgcaa
     2881 gtttaacaag cacaacatta aagcttgaaa tgagcacttc ttgattctga tttaagtttg
     2941 ttaattgttc ttccacttgc ttaaccatac ttttagaaag tgctagtcca ttcggtccag
     3001 taataccttt taattctaca tttaaattca tattatattc atttgctatt tttactacat
     3061 catcgaaagt tggcaaatgt tcatctttga atttttcacc aaaccaagat cctgcagaag
     3121 catctttaat ttcatcataa ttcaattcag ttatttcccc ggacatattt gtagtccgtt
     3181 ctaaataatc atcatgaatg ataatcagtt gttcatcttt tgtaattgca acatctaact
     3241 ccaaccagtt tataccttct acttctgaag cagctttaaa tgatgcaatt gtattttccg
     3301 gagctttact aggtaatcct ctatgtccat atacagttag catattacct ctccttgcat
     3361 ttttattttt ttaattaacg taactgtatt atcacattaa tcgcactttt atttccatta
     3421 aaaagagatg aatatcataa ataaagaagt cgatagattc gtattgatta tggagttaat
     3481 ctacgtctca tctcattttt aaaaaatcat ttatgtccca agctccattt tgtaatcaag
     3541 tctagttttt ctgtacccct tatctgcaat tttacttagg attgctttta acttacccct
     3601 tatcagcaat tttactgaga actgctttta acgcacctct tatctgcaat tttgcctaga
     3661 actgctttta acgtacctct tatctgcaat tttactgaga actgctttta acttacccct
     3721 tatcagcaat tttgcatgga attgctttta acgtacctct tatctgcaat tttacttaga
     3781 actgctttta acaaacctct tatctgcaat tttactt
//
LOCUS       DALAGF010000269         3522 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.guided.159, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000269 DALAGF010000000
VERSION     DALAGF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.guided.159"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            99..1856
                     /gene="blaR1"
                     /locus_tag="O2692_002880"
     CDS             99..1856
                     /gene="blaR1"
                     /locus_tag="O2692_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCZ4300426.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            1846..2226
                     /gene="blaI"
                     /locus_tag="O2692_002881"
     CDS             1846..2226
                     /gene="blaI"
                     /locus_tag="O2692_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCZ4300427.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            2490..3068
                     /locus_tag="O2692_002882"
     CDS             2490..3068
                     /locus_tag="O2692_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCZ4300428.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(3291..3476)
                     /locus_tag="O2692_002883"
     CDS             complement(3291..3476)
                     /locus_tag="O2692_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300429.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
BASE COUNT         1407 a          422 c          533 g         1160 t
ORIGIN      
        1 ctccgatatt acagttgtaa tttttataaa tcaataatat tacaattgta atattgatgt
       61 caattgttta ttataaatat ttgaaagaag gtgtcaaaat ggctaaatta ctaataatga
      121 gcgtagtaag cttttgtttt atatttctat tgttagtatt ttttaggtac atattaaaac
      181 gctattttaa ttatagttta aattataaag tttggtatct aactatgctt gctggattaa
      241 ttccttttat cccaattaaa ttctctttta taaaatttaa taatgtgaat aatcaagcgc
      301 ccacagttga aagtaagtca cacgatttga accataacat aaataccacc aaacctattc
      361 aagagttcac aacagatatc cataagttta attgggattc aattgataat atctgcacag
      421 ttatttggat agttttagtt attattttaa gttttaaatt tttgaaagcc ttattatatc
      481 ttaaatattt aaagaaacag tcactttatc taaacgaaaa tgaaaaaaat aaaatagata
      541 cgatactttt caaccatcaa tataaaaaaa atattgtgat tcgaaaagca gaggctattc
      601 aatctccaat aactttttgg tatgggaaat atattatttt gattcctagt tcatatttta
      661 aaagtgtaat tgataaaaga ctaaaatata taatactgca tgaatatgct catgctaaaa
      721 atagagatac tttacattta attatcttta atatcttctc tattattatg agttacaatc
      781 cattagtaca tattgtaaaa agaaagataa ttcatgacaa tgaagtagaa gccgatagat
      841 ttgtccttaa taatattaac aaaaatgaat ttaaaactta tgcggaatct attatggact
      901 ccgtattaaa aaccccattt tccaacaaaa atatattaag ccattcattt aatggtaaaa
      961 aatcattact caaaagtaga ttaattaata taaaagaagc cgatctgaaa aagcagtcaa
     1021 aattaattct aatttttatt tgtattttta ctttttttat catgataatt caaagccagt
     1081 ttttaatggg gcaatcctta actgattata attataaaaa gcccttacaa agcgattacc
     1141 aaattttaga tgaaagtaaa aattttggtt caaatagtgg atctttcgtt atgtacagca
     1201 tgaagaaaga caaatactat atttataatg aaaaagaaag tagaaaaaga tattctcctg
     1261 attcaactta caaaatttat ttagcactgt ttggacttga ccgtcatatt ataagtgata
     1321 aaaattcacg tatgtcatgg aatcataaac attatctttt cgagtcttgg aataaggaac
     1381 aagatttaaa tacagcaatg caaaattcag ttaattggta cttcgaacgt attagtaatc
     1441 aaataccaaa gaactatact gcggctcaac tcaagcaatt aaattatggt aatgaaaatt
     1501 tgggaagtta taaaagctat tggatggaag atagtttgaa aatatctaat cttgaacaag
     1561 taatagtttt taaaaatatg atggaacaaa ataaccattt tagtaaaaaa gcaaagaatc
     1621 aattatcttc ttcattattg attaagaaaa atgaaaagta tgaactgtat gggaaaacag
     1681 gtacaggtat agtaaacggg aagtataata atgggtggtt tgtaggttac gtaattacaa
     1741 atcatgataa gtattatttt gctacacatt tatcagatgg aaacccgtct gggaaaaatg
     1801 ctgaattaat aagtgaaaaa atattaaaag gaatgggtgt tttaaatgac caataagcaa
     1861 gttgaaatat ctatggctga atgggatgtt atgaatataa tatgggataa aaaatcagta
     1921 tcagctaatg aaattgtagt tgaaattcaa aaatataaag aagttagtga taaaacgatt
     1981 agaacattaa tcacaagact atataaaaaa gagattataa aacgatacaa atcagaaaat
     2041 atttattttt actcatcaaa tattaaagaa gacgatatta aaatgaaaac tgctaaaacc
     2101 tttcttaata aactgtatgg aggggatatg aaaagtttag tgctgaattt tgcgaaaaat
     2161 gaagaattaa ataacaaaga aattgaagaa ttgcgagaca ttttaaatga tattagtaaa
     2221 aagtaattag aggtatgttt caaacataat acatatgtaa accatacttg actcttatta
     2281 aattagtttt gttaatgaca tttaaaatat attattaaag atgagagtgt ccagaaaata
     2341 accattttct gtacactctt gttttttgtc taaataagta agtattacat agaattatgt
     2401 gttcaaaaac ccaacaaaaa atctatgttc acaaaagacc tcgtaatcaa gttatggtac
     2461 actaaaggtg tatataggag gtctgtattt tgaaaatagg ttatgcaaga gtttcaactg
     2521 gattacaaaa tttgaattta caggaggatc gattaaatac atatggttgt gaaaagatat
     2581 ttaatgacca tatgagtggt tcaaaaagta agcgacctgg tttagataaa gcaattgaat
     2641 ttgcgaggtc aggagatacg attgttgttt ggagattaga tagactagga cggaacatgg
     2701 aggatttaat cacgttagtt aatgaactta acgaacgagg tgtgagtttt catagtttag
     2761 aagaaaatat aacgatggat aaatcaagtt ctacaggaca attattattt catttattcg
     2821 cagcatttgc agagtttgaa cgtaatttaa ttttagagcg atcatcagcc ggtcgaattg
     2881 cagcacgtgc tagaggccga tatggaggta gacctgaaaa attaaataaa caagatttaa
     2941 acttacttaa gacactttat gataatggta cgccaattaa aacaattgca gagcagtggc
     3001 aagtttcacg tacaactatt tatcgttatc tcaataaatt agaggcaaaa gagaataaaa
     3061 acaagtagag caactaatct agcttactat aatgaatcta ctaaataatt ttatatatca
     3121 ttaacataca aagactttga ttcaatgtca ctcgatgtaa aagagacgtt ctgacagcgt
     3181 ctaaaatctc ataatatttt cctgttatga gattttaata ataacacaag aaaaaagcgt
     3241 acaaattcgt acgctttttt aatataatac ctgtttcact aatgaacgtt ttaagtatct
     3301 tcatcattaa ctgaaactac agtaataata ctcgtcaaca atattagtat agttaaaata
     3361 gaaagactta ggactggcat aaccgaacta aaattatatt tgaaatataa aagtgctaat
     3421 gaagagagaa atgtaaaaat aataaatacc aaagaccatt tttctaattt attcattggc
     3481 ttcacctctt tgaactttta tataatcata ggagatattt tt
//
LOCUS       DALAGF010000270         3300 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.guided.164, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000270 DALAGF010000000
VERSION     DALAGF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.guided.164"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            40..417
                     /locus_tag="O2692_002884"
                     /pseudo
     CDS             40..417
                     /locus_tag="O2692_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            414..1295
                     /locus_tag="O2692_002885"
                     /pseudo
     CDS             414..1295
                     /locus_tag="O2692_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1411..2340
                     /locus_tag="O2692_002886"
     CDS             1411..2340
                     /locus_tag="O2692_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCZ4300430.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2333..3070
                     /locus_tag="O2692_002887"
     CDS             2333..3070
                     /locus_tag="O2692_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCZ4300431.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3084..3287
                     /locus_tag="O2692_002888"
     CDS             3084..3287
                     /locus_tag="O2692_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCZ4300432.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLGGSVAKLNL"
BASE COUNT         1201 a          433 c          587 g         1079 t
ORIGIN      
        1 tattagtgat tagctatatt ttttactttg caacagaacc agccatttaa tatgactgtt
       61 acaattacta agattcaagc actattacgc cgcacgtatg atttcaccac tcaagttaat
      121 aatttaacag tagatgattg cgagttacta cttgatgcag caaagcttaa atttaatgaa
      181 gaaaccatag acctgacaca cactgaattg caaatcatgc atagcttatt ccagaacaag
      241 ggcaaattcg tcagtcgtaa tgcgttaatc gaaaaatgct gggaatcaga acacttcatt
      301 gatgacaata cgcttgctgt caacattaca cgtttacgca aaaagctaca aaaactagga
      361 cttacagatt ttatcgaaac gaagaaaaat gtcggatatc gtgtaggtga cgtatgaatt
      421 tcttgaaatc aatccaacgt gaaatttata ttgtcatcct tacttgtgct ttattctact
      481 ttatattatt catgtataat gtgccgatgc aagcatatat gattggtcta ggtattgtat
      541 tgtttgtttc aatgatttac tggggtatcc attacatgaa ctttaaaaaa gaataaacac
      601 tacaggaaca gaacgaagat ttaagaatcg aaaaccataa acttcgtaat gactttatta
      661 actaccaaag tgagttagag tcatattttt taatgtgggt gcatcaaatc aagacaccga
      721 ttacagcatc aaagttactg cttgaagatc ctgatgaaca cgtgatttct aacgtgcgta
      781 atgaagtctt acaaattgat aattacacga atcttgcact gaactatttg aaactcatga
      841 accaaaagac atacatggtc tttatggaag tgagtgtaga tgaattaatt cgaccactga
      901 ttaaacgtta tgcgatacag tttattcatc atcaaacaaa gttgcattat gagaaaagtg
      961 aagcggttat attaacagat gccaaatggg catcaattat gattgagcaa atattaaaca
     1021 atgcattaaa atatgcacgt ggcaaagatg tgtggattac gtttgacgaa aatacaaaga
     1081 aattatcaat ccgagataac ggtatcggca ttaatgcgag tgatttaccg aaaatctttg
     1141 ataaaggttt ctcgggctat aacggtagaa taagtgagaa atcaagcggt attggtttat
     1201 atattgtcag cacaatctca aagcgcttag gccataaagt aagtgctaca tctacattaa
     1261 acgatggttc aatattcacc attcaattta aataactcca atctttcatt tttgtaagat
     1321 tagcggctgt ttttgtaaga atagataaag gcagcctttt ctaatcgcgc gtaagataaa
     1381 ggtaacaaca aactaagaaa ggtgccagag gtgaaaaatt tgaataatat aattgaaata
     1441 aataacttaa caaagcaata tggaagtcag atagcagtta ataatttgag tataactgtt
     1501 gaaagtggac aaatttatgg ttttttagga agaaatggtg caggcaaaac aactactata
     1561 agaatgcttt taggcttaat taaacctacg aaaggtaata ttaaaatatt tgaaaaagag
     1621 ttcgaaagta ataaaactga aattttaaaa aatattggtg ctatggttga aacgccaggt
     1681 ttttatagta atttgacagc taaagagaac ttaactataa atgctaaaat attaggtata
     1741 caaaaagaaa atgcaattga agaagtgtta gaaatagtag gactagcttc tgaaaaagta
     1801 aaattgttta aaaattattc tttaggtatg aagcaacgtt tagggattgc gagagcttta
     1861 ttgcacaatc ccgaattatt gatacttgat gagccgacga atggattaga tcctattggc
     1921 ataaaagaaa ttagaaagtt gattaaggat ctggctcaat ctagaaatat aaccatttta
     1981 atttcaagtc atatattgtc agaaatagag cagttagtag acaaggtggg tattattcat
     2041 aacggagttt tgttagaaga aattatgtat gaagatttaa aaaaaaagaa cagacaattt
     2101 ataagtatat cagtatcaga tgaagaaaaa gttgcatgtt tgttggaaac taacttcgga
     2161 atttatgact ataaaattaa agaaaatggc gaatttaaaa tttattcaca tattgataga
     2221 caacaagaaa ttaatagatt attagtatta aacaatattg atgttttcca gatgagagtg
     2281 atagaagata atctagaaac ttatttcgaa gacttagtag gaggtggcac aattggataa
     2341 tctactccgt acagaattta ttaagttaaa aagatcatat atgtttctaa taagtatttt
     2401 aggtacatgc gtagcacctt ttatggtggt tgtagcttct tttattagta tgcggacgaa
     2461 agtaccgact cctaatattg tatttaaaca gttatttgaa gaagtcagtt tatatcatat
     2521 gctaattatt ggacctcctc tatatggggt tataatagcc tatttgtttg gaagagaata
     2581 tatagaagat actttaaaaa acttattaat tatacctata tctagagaaa gttttttagt
     2641 aagtaaaata atattattat tcttgtggat tcaaacacta tctgttattg catggttgct
     2701 aactttaatg ctaggtatgc tattgcattt tcaagggtta agccagagtt taataattga
     2761 ttcattcttt acatttgtta ttggcggagt gttattatgg atattatctc caccaataat
     2821 tattttgtcg atatatttta aaaattatgt tcccacaatt gttttaacca taataattac
     2881 tttgataaat ttaatgactg ctaattctga acatagagga ttatttcctt gggctgcagt
     2941 tggagacata tctagaggaa cattacctaa tacttatcca tttctattat ctttaattat
     3001 cataattgtt acatctatat tcagtgtaat tatattattc tcatacttta aaaaaactga
     3061 tataagttaa aaggaggaga aaaatgggtt ttatagagca gattaaagca tttattttag
     3121 gtgtagttga agggttgaca gaatttgctc cagtatcatc aacaggtcat atgattattg
     3181 ttgatgatat gtggttaaac actgctgacg atttaggaag tgcgtctgca actactttta
     3241 aggtggtaat acagttagga ggttctgttg caaagttgaa tttataatat aattttaaca
//
LOCUS       DALAGF010000271         2421 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.guided.182, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000271 DALAGF010000000
VERSION     DALAGF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.guided.182"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            214..921
                     /gene="deoD"
                     /locus_tag="O2692_002889"
     CDS             214..921
                     /gene="deoD"
                     /locus_tag="O2692_002889"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCZ4300433.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            928..2280
                     /gene="tet(38)"
                     /locus_tag="O2692_002890"
     CDS             928..2280
                     /gene="tet(38)"
                     /locus_tag="O2692_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCZ4300434.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVRLVLFVSLNRWTQSEK
                     "
BASE COUNT          785 a          351 c          435 g          850 t
ORIGIN      
        1 catacaataa tttttactca aattggaaaa attataaaaa ttaaatatag ataggctttg
       61 aaaattagtt ttatacaagg ttagtagctg taactgtaaa atgttcttaa tattgtcaaa
      121 atgtaatgct tgaaagcgct tttaaaaaat attattatat acatggttag acaaatagac
      181 aaatcactat acaaatattg ggaggaatat tttatgaaat caacaccaca cattaaacca
      241 atgaatgacg tcgaaattgc agaaacggtt ctattgccag gagatccgtt aagagctaag
      301 ttcattgcag aaacttattt ggatgatgtg gaacagttca atacagtgcg aaacatgttt
      361 ggttttaccg gaacatataa aggtaaaaaa gtttctgtca tgggttcagg tatgggtatg
      421 ccatctattg gcatttactc ttatgaatta attcatacat ttggttgtaa aaaattaatt
      481 cgcgttggct cttgtggcgc gatgcaagaa aacattgatt tatatgatgt gattattgca
      541 caaggtgcct ctactgattc aaattacgtt caacaatatc aattaccagg tcattttgcg
      601 ccaattgctt cttatcaatt attagaaaaa gcagttgaaa cagcacgtga caaaggtgta
      661 cgtcatcatg taggtaatgt gttatcaagt gatattttct ataacgcgga tacaacagcg
      721 agtgaacgtt ggatgcgtat gggtatttta ggtgtagaaa tggaatcagc tgcattatac
      781 atgaatgcaa tttacgctgg tgtcgaagca ttaggtgtgt tcacagtgag cgatcattta
      841 attcatgaaa cgtcaacaac acctgaggaa agggaacgtg catttacaga tatgattgaa
      901 attgcactgt cattggtgta gatgattatg aatgttgaat attctaaaat aaagaaagca
      961 gtacctattt tattattctt atttgtattc agtttggtta tagacaactc atttaaattg
     1021 atttctgtag ccattgctga tgacttaaac atatctgtaa cgacagtaag ttggcaagcg
     1081 acattagccg gtttagtaat tggtattggc gctgtagtat acgcttcatt atctgatgcc
     1141 attagtatac gcacactatt tatttatggc gtgatattaa tcattatcgg atcaattatt
     1201 ggttacattt tccaacatca attcccatta cttttagttg gacgtattat tcaaactgcc
     1261 ggtttagctg ctgcagagac attatatgtg atatatgttg caaagtatct ttctaaagag
     1321 gaccagaaga cttaccttgg cttaagtacg agcagttatt ccttgtcatt agttatcggt
     1381 acattatcag gtggatttat ttctacgtat ttacactgga caaatatgtt tttaattgca
     1441 ttaatcgtag tatttacgtt gccattccta tttaaattat taccaaaaga aaataatacg
     1501 aataaagctc atttagattt tgttggctta attctagtgg caactattgc tacaacagtc
     1561 atgctgttta ttacgaactt taattggtta tatatgattg gtgccttaat tgcgattatc
     1621 gtttttgcgc tatatattaa aaatgcgcaa cgtccattag taaataaatc atttttccaa
     1681 aataaacgtt atgcttcatt tttatttata gtatttgtaa tgtatgctat ccaattgggt
     1741 tatattttta cgttcccatt cataatggag caaatttatc atctgcaact agacacaaca
     1801 tcactgttat tagtaccggg ttatatagta gcagtcattg ttggtgcact aagtggtaaa
     1861 atcggcgaat atctgaattc aaaacaagcg attatcacag caattatttt aatagcactg
     1921 agcttgattt tacctgcatt tgcagtaggt aatcacattt caatcttcgt catttctatg
     1981 atattctttg caggtagctt tgctttaatg tatgcacctt tacttaacga agccattaaa
     2041 acaatagatc ttaatatgac aggtgtggct attggttttt ataatttaat tattaatgtg
     2101 gcggtatctg taggtattgc gattgctgcg gctctaatcg attttaaagc attaaatttc
     2161 ccaggcaatg atgcattaag ttcacatttc ggtattattt taattatttt aggtttaatg
     2221 agtattgtca gattagtttt attcgtcagc ttaaatcgtt ggacacaatc tgaaaaataa
     2281 atagatataa attcgcgaga tatattcgta tttatagtaa aattaaataa agagattata
     2341 taacacgagg agtagtaagt atgaaatttg agaaatatat agatcacact ttattgaagc
     2401 ctgagtcaac acgtacgcaa a
//
LOCUS       DALAGF010000272         2005 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.guided.197, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000272 DALAGF010000000
VERSION     DALAGF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.guided.197"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            233..1027
                     /locus_tag="O2692_002891"
                     /note="aph(3')-IIIa"
     CDS             233..1027
                     /locus_tag="O2692_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCZ4300435.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1303..1874
                     /locus_tag="O2692_002892"
                     /pseudo
     CDS             1303..1874
                     /locus_tag="O2692_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT          656 a          320 c          471 g          558 t
ORIGIN      
        1 tattagtgat tagctatatt ttttactttg caacagaacc ttgtaaattc tatcataatt
       61 gtggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aaagctgttt
      121 tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat
      181 tagcttcttg gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa
      241 aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac
      301 ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt
      361 aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat
      421 gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga
      481 tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga
      541 agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca
      601 ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt
      661 ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac
      721 tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga
      781 acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt
      841 aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt
      901 ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga
      961 cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt
     1021 gttttagtac ctagatttag atgtctaaaa agctttaact acaagctttt tagacatcta
     1081 atcttttctg aagtacatcc gcaactgtcc atactctgat gttttatatc ttttctaaaa
     1141 gttcgctaga tagagttcta tattagagat gtaaagagtt ttggtgaaga ggtttgtgag
     1201 tatggctttt ataaccaggg tgcaatgaga agcactaaca gcagctagct taagaacgca
     1261 taggatatac tttttatgga agtcacagag gaggaataaa atttgaacag gattaacaga
     1321 gtaacctcta ttcttattca gctgcaatca aaaaaataat tcctgccaaa gaaattgcac
     1381 agcgatttaa tataagctta aggacagttt acagagatat ccggacactt gaagaagccg
     1441 gaataccaat tggatctgag gccggaaaag gatattttct tgtagagggc ttcctacttc
     1501 tgccggtaat gtttacagcg gcggaagtgg gtgcattgat tacagcaggg aaatttttaa
     1561 attgccatgg agatgaatca tttataaagg attttgattc agctatgtat aaaatcaaat
     1621 ctattttgaa gcatggtgaa aaaaactatg cacaggagtt ggagaacagt attaacgtgt
     1681 acagcacatc tggacagaaa aatacattgg cggataacgt tattgcagca attcagactg
     1741 caatctgcaa taaaagggta atatcaattc aatatcctgc atcaggaggg caagaaccgg
     1801 aaagcagaat gatagagcct atatcactgg ggttttatga acagaactgg tacttaatcg
     1861 gttttgcagg ctaagaaatg aatataggaa ttttcgtgtg gatcggatta aaagcatttg
     1921 catagaagaa gagctctgtc aaagccatga tggctctttg gaacaaatat taaaacaaat
     1981 gctggttctg ttgcaaagta aaaaa
//
LOCUS       DALAGF010000273         1560 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.guided.208, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000273 DALAGF010000000
VERSION     DALAGF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.guided.208"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            98..1084
                     /locus_tag="O2692_002893"
     CDS             98..1084
                     /locus_tag="O2692_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCZ4300436.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            1056..1316
                     /locus_tag="O2692_002894"
     CDS             1056..1316
                     /locus_tag="O2692_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCZ4300437.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(1307..>1560)
                     /locus_tag="O2692_002895"
     CDS             complement(1307..>1560)
                     /locus_tag="O2692_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCZ4300438.1"
                     /translation="VFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAE
                     NNQKIYKKDNFYIISIEIVFFHLSKNFMSRTHYDLFFYC"
BASE COUNT          561 a          215 c          256 g          528 t
ORIGIN      
        1 atatactcct tatataagac tacatttgta gtatattaca aatgtagtat ttatgtcaaa
       61 ataatgttat aatttttgtg atatggaggt gtagaaggtg ttatcatctt ttttaatgtt
      121 aagtataatc agttcattgc tcacgatatg tgtaattttt ttagtgagaa tgctctatat
      181 aaaatatact caaaatatta tgtcacataa gatttggtta ttagtgctcg tctccacgtt
      241 aattccatta ataccatttt acaaaatatc gaattttaca ttttcaaaag atatgatgaa
      301 tcgaaatgta tctgacacga cttcttcggt tagtcatatg ttagatggtc aacaatcatc
      361 tgttacgaaa gacttagcaa ttaatgttaa tcagtttgag acctcaaata taacgtatat
      421 gattcttttg atatgggtat ttggtagttt gttgtgctta ttttatatga ttaaggcatt
      481 ccgacaaatt gatgttatta aaagttcgtc attggaatcg tcatatctta atgaacgact
      541 taaagtatgt caaagtaaga tgcagttcta caaaaagcat ataacaatta gttatagttc
      601 aaacattgat aatccgatgg tatttggttt agtgaaatcc caaattgtac taccaactgt
      661 cgtagtcgaa accatgaatg acaaagaaat tgaatatatt attctacatg aactatcaca
      721 tgtgaaaagt catgacttaa tattcaacca gctttatgtt gtttttaaaa tgatattctg
      781 gtttaatcct gcactatata taagtaaaac aatgatggac aatgactgtg aaaaagtatg
      841 tgatagaaac gttttaaaaa ttttgaatcg ccatgaacat atacgttatg gtgaatcgat
      901 attaaaatgc tctattttaa aatctcagca cataaataat gtggcagcac aatatttact
      961 aggttttaat tcaaatatta aagaacgtgt taagtatatt gcactttatg attcaatgcc
     1021 taaacctaat cgaaacaagc gtattgttgc gtatattgta tgtagtatat cgagcttcac
     1081 atgaaacagc taaagaagct ttgggcgata aagagttaag agccattgca catgagttaa
     1141 ctaaaacagt taaggataac atgagtgttg attggtctaa acgagacagt gctaaagcta
     1201 aaatgagagt tcaagttaga cgcctattaa agaaatatgg ctatccacca gatcttcaaa
     1261 aaatggctgt ggaacaagtt gtagagcaag cagaattaat ggcaagtcag caataaaaaa
     1321 ataaatcata atgagtccgg gacataaagt tcttggataa gtgaaaaaag acaatttcta
     1381 ttgaaataat atagaaattg tcttttttat aaattttttg attattttca gctcgttgag
     1441 ctactacttt tcttatatta agtgccatta atacaaaacc aagttctctt ttgactttat
     1501 tgagtcctcg gacagacatc cgagtgaaac ccaaaatagc cttcataaat ccaaaaacag
//
LOCUS       DALAGF010000274          967 bp    DNA     linear   BCT 14-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus strain ST8 isolate Sample 214 from
            subject 474 SAMN14154632-rid16264533.guided.224, whole genome
            shotgun sequence.
ACCESSION   DALAGF010000274 DALAGF010000000
VERSION     DALAGF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14154632
            Sequence Read Archive: SRR11139380
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001525642.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001525642.1
            Genome Coverage       :: 147x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2022 03:50:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,855
            Genes (coding)                    :: 2,787
            CDSs (with protein)               :: 2,787
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 36 of 68
            Pseudo Genes (incomplete)         :: 28 of 68
            Pseudo Genes (internal stop)      :: 12 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14154632-rid16264533.guided.224"
                     /strain="ST8"
                     /isolate="Sample 214 from subject 474"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2017"
                     /collected_by="Cook County Health"
     gene            47..892
                     /gene="blaZ"
                     /locus_tag="O2692_002896"
     CDS             47..892
                     /gene="blaZ"
                     /locus_tag="O2692_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCZ4300439.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
BASE COUNT          399 a          108 c          149 g          311 t
ORIGIN      
        1 attattgatt tataaaaatt acaactgtaa tatcggaggg tttattttga aaaagttaat
       61 atttttaatt gtaattgctt tagttttaag tgcatgtaat tcaaacagtt cacatgccaa
      121 agagttaaat gatttagaaa aaaaatataa tgctcatatt ggtgtttatg ctttaaatac
      181 taaaagtggt aaggaagtaa aatttaattc agataagaga tttgcctatg cttcgacttc
      241 aaaagcgata aatagtgcta ttttgttaga acaagtacct tataataagt taaataaaaa
      301 agtacatatt aacaaagatg atatagttgc ttattctcct attttagaaa aatatgtagg
      361 aaaagatatc actttaaaag aacttattga ggcttcaatg acatatagtg ataatacagc
      421 aaacaataaa attataaaag aaatcggtgg aatcaaaaaa gttaaacaac gtctaaaaga
      481 actaggagat aaagtaacaa atccagttag atatgagata gaattaaatt actattcacc
      541 aaagagcaaa aaagatactt caacgcctgc tgctttcggc aagactttaa ataaacttat
      601 cgcaaatgga aaattaagca aaaaaaataa aaatttctta cttgatttaa tgtttaataa
      661 taaaaacgga gacactttaa ttaaagatgg tgttccaaaa gactataagg ttgctgataa
      721 aagtggtcaa gcaataacat atgcttctag aaatgatgtt gcttttgttt atcctaaggg
      781 ccaatctgaa cctattgttt tagtcatttt tacgaataaa gacaataaaa gtgataagcc
      841 aaatgataag ttgataagtg aaaccgccaa gagtgtaatg aaggaatttt aatattctaa
      901 atgcataata aatactgata acatcttata gtttgtatta tattttgtat tatcgttgac
      961 atgtata
//
